LOCUS       DAUDYX010000001       110483 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.001, whole genome shotgun sequence.
ACCESSION   DAUDYX010000001 DAUDYX010000000
VERSION     DAUDYX010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 110483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 110483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..110483
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.001"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            77..718
                     /locus_tag="VHD02_000001"
     CDS             77..718
                     /locus_tag="VHD02_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEQ5496644.1"
                     /translation="MKDLLKQYQAKPLGEEKRYAVFLPLILVNDDWHVLYEVRSQHIS
                     QPGEVSFPGGQVENQETLQEAAIRETVEELTVDASQIQLWGEIDYLVQSSRTIHCFVG
                     QLVVDDWESIQPNEEVDKVFTVPLRQLLVTDPVYYHLEATPIETTDFPFDRIRNGKYY
                     QFSQEYRSIPFYENLEETIWGMTAQFTKCLTDILNDESIAKKNPLDIEKSKGR"
     gene            complement(765..1607)
                     /locus_tag="VHD02_000002"
     CDS             complement(765..1607)
                     /locus_tag="VHD02_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEQ5496645.1"
                     /translation="MVSFISRVFKGMIIALGFILPGVSGGVLAAILGIYERMISFLAH
                     MRDNFIENVLFFLPVGIGGILGIALFSFPVEFLLKHYQVIVLWGFAGAIVGTIPSLIK
                     ESTKQSQRDKVDWLWLVLTFVISGLGLYFLNDLIGTLPANFLTFILAGALIALGVLVP
                     GLSPSNLLLILGLYGPMLIGFKSLDLLGTFLPIAIGGVLAILAFSKSMDYALQHHHSK
                     VYHFIIGIVLSSTLLILIPNSSSPESISYSHAGILTWLMAVVLFALGIWLGLWMSQLE
                     EKYK"
     gene            2010..2054
                     /locus_tag="VHD02_000003"
                     /pseudo
     CDS             2010..2054
                     /locus_tag="VHD02_000003"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2061..2342
                     /locus_tag="VHD02_000004"
     CDS             2061..2342
                     /locus_tag="VHD02_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ5496646.1"
                     /translation="MLSKSLQVPLEQLIAEDIPKMTLIIKDEEIKQCDRNTHIMLFGT
                     LLIAISIYPIMSWLGWWGYGPVAILWLIIMRSALRIERFKKTYDVQTYR"
     gene            complement(2722..3324)
                     /locus_tag="VHD02_000005"
     CDS             complement(2722..3324)
                     /locus_tag="VHD02_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496647.1"
                     /translation="MTTKTYFMRSFNLILYLLLIAPICYVFGWLFNLVSVQLSTVTFV
                     NLDTVKNVTDTISSVCYGFALISLLLALLSGLKMIQRLNTDSLGNYCQSIYRTFLLRH
                     FLFQRERVQKVSNFEHQTVTTINPIHNGFNRAVRKCIVDIRKDAITVFLKVPRDQQGQ
                     KILKDTGVQLKEEIVIQHTDYYFSSPVRVRNQLWFIGQKR"
     gene            complement(3321..4073)
                     /locus_tag="VHD02_000006"
     CDS             complement(3321..4073)
                     /locus_tag="VHD02_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEQ5496648.1"
                     /translation="MAIPDLDKFSSNLQQGKIPVIKHFELNLDKGSHWAIAGNSGSGK
                     PYALTYFLSVLKPMSGLIIIDPKFDTPSQWARENKIAVIHPVENHSKSDFVSQVNEQL
                     NQCATLIQKRQAILYDNPNHQFTHLTIVIDEVLALSEGVNKNIKEAFFSLLSQIALLG
                     HATKIHLFLGSQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPTG
                     HGTGIIQVVDNEHSYQVLPLLCPTYYTKRGIL"
     gene            complement(4139..5062)
                     /locus_tag="VHD02_000007"
     CDS             complement(4139..5062)
                     /locus_tag="VHD02_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ5496649.1"
                     /translation="MKFFEENYSQEIPTRIKNLRKKHNITQSELGNAGQVSQVESGKR
                     PITSSMLVYLNALTASSYTYIVFGELDEFIENLFHYFFSSILYRDLEAVDENLYSFMS
                     DDLISIQSSCLSIAKTFANFNIQRKRFMISTETEMDTFHKKDDIDVWVGGKSYNPARS
                     FRNNPINELTVIDFEEMADILLLMLRDNLIRSFEINVCNTLFELDKNGEPTTFNLDKI
                     DSIINKWWSENVSTEIIPNLIKKLRENPLFNIGFMVNDILERMYKEDIPKSYLTSVPL
                     VISKKARSTLSIKSNGSQKIDKSKFAQILSL"
     gene            complement(5113..5451)
                     /gene="cadX"
                     /locus_tag="VHD02_000008"
     CDS             complement(5113..5451)
                     /gene="cadX"
                     /locus_tag="VHD02_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HEQ5496650.1"
                     /translation="MKKDSICQVDVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLAVEELCVCDIACLLNLSMASASHHLRKLANQNILDSRREGKIIYYFIKDE
                     EIRDFFNQLG"
     gene            complement(5463..6089)
                     /locus_tag="VHD02_000009"
     CDS             complement(5463..6089)
                     /locus_tag="VHD02_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEQ5496651.1"
                     /translation="MRYFMIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIY
                     LGQFLGSVSLIFLSLLFAFVLNYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKD
                     GLRKDNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVTLLTFLVMIYLLVFS
                     AQKLAQVPSVGETLEKYSRWFIAVVYLGLGMYILIENNSFDMLWAVLG"
     gene            complement(6370..6519)
                     /gene="rpmG"
                     /locus_tag="VHD02_000010"
     CDS             complement(6370..6519)
                     /gene="rpmG"
                     /locus_tag="VHD02_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEQ5496652.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(6535..6717)
                     /gene="rpmF"
                     /locus_tag="VHD02_000011"
     CDS             complement(6535..6717)
                     /gene="rpmF"
                     /locus_tag="VHD02_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEQ5496653.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            6937..8217
                     /locus_tag="VHD02_000012"
     CDS             6937..8217
                     /locus_tag="VHD02_000012"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEQ5496654.1"
                     /translation="MKLQKPKGTQDILPGDAAKWQYVESVARDTFSQYNYGEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PVKLYYIGSMFRYERPQAGRLREFHQIGVECFGAANPATDVETIAMAYHLLEKLGIKD
                     VTLHLNSLGSPESRSAYRQTLIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLA
                     VEKAPSILDYLDEESQAHFEAVKAMLEALDIPYVIDTNMVRGLDYYNHTIFEFITSVE
                     GSDLTICAGGRYDSLVGYFGGPETPGFGFGLGLERLLMIIEKQGLALPIETEMDVYLA
                     VLGDGANSKALELVQAIRRQGFTAERDYLGRKIKAQFKSADTFKAKLVMTLGESEVEA
                     GKAVIKNNRSRQEVEVSFEDMMTNFANISEQLLS"
     gene            8555..10303
                     /gene="aspS"
                     /locus_tag="VHD02_000013"
     CDS             8555..10303
                     /gene="aspS"
                     /locus_tag="VHD02_000013"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEQ5496655.1"
                     /translation="MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREG
                     VMQLVINPEEVSSDVMATAERLRSEYVIEVEGFVEARQQANDKLATGMVELKVSALTI
                     LNTAKTTPFEIKDDVEVSDDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEF
                     IDVETPMLTKSTPEGARDYLVPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSEQEIQDIVEGMIATVMKETKEIDVTLPFPRMSY
                     DVAMNSYGSDKPDTRFEMLLQDLTVTVKGIDFKVFSEAPAVKAIVVKGNADRYSRKDI
                     DKLTEFAKQFGAKGLAWVKVTDGQLAGPVAKFLTAIETELSSQLKLAENDLVLFVADT
                     LEVANNTLGALRNRIAKDLDMIDQSQFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     PESAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKEMQERMFKALGFTADEANAQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMTQAPSLV
                     SENQLEELSLQIESHD"
     gene            10296..11237
                     /locus_tag="VHD02_000014"
     CDS             10296..11237
                     /locus_tag="VHD02_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ5496656.1"
                     /translation="MIKKTTYKKKVKYVISRGAKKVGLLHALRSISREKYAEKISASL
                     LYGILSSIAVNFFFQPGHVYSSGATGLAQVFSALSHRLLGYDFPIAFAFYLINIPLLI
                     LAWYKIGHQFTIFTFITVSMSSFFIQIIPQVTLTTDPLINAIFGGLVMGMGIGTGLKS
                     RISSGGTDIVSLTLRKRTGKDVGSLSLMVNGAILAFAGILFGWQYALYSMVSIFVSSR
                     VTDAIFTKQKKMQATIVTSHPERVIHMIHKRLHRGVTSINDAEGTYKHEQKAVLITIL
                     TCEEYPEFKWLMLKTDPQAFVSVAENVRIIGRFVEDD"
     gene            11257..12135
                     /locus_tag="VHD02_000015"
     CDS             11257..12135
                     /locus_tag="VHD02_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ5496657.1"
                     /translation="MSIDQRLQLKKFCFIILGAAIYAFAFVYFYMANRIAANGLAGLT
                     LVGKALFEIDPSLIGYLINLPLVLLGARCFGKRAMVYTVTGILSLYFFVWLFQRFPLV
                     VDLDHDNLVVSLMAGIIGGLGGGLVFRNGGTIGGADIIAKLLEDKLGLQLNQALLGID
                     LFVMIVSLTYISLPQMMYALIASFMYGQIVRLVQQGGYSARGIFIVSEQAEKIARFIM
                     DELGRGVTYLTGEGAYSGRRKKVIYVALGRRDIRELKAFLTQVDPNAFITYFDVNEVN
                     SPEFLTIKSKYHKKRK"
     gene            12178..13050
                     /locus_tag="VHD02_000016"
     CDS             12178..13050
                     /locus_tag="VHD02_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ5496658.1"
                     /translation="MAHHDKLTKLLKLFLIALGVAIYTFGFVNFNMANALAEGGVAGI
                     TLILHAHFGINPAYSSLLFNLPLFILGAKIFGKRSLALTIYGTVLMSAFIWMWQKVPI
                     ELGLENDMMLVAVVAGLFSGIGSGIVFRYGATTGGTDIIGRIAEEKFGAKLGQTLLLV
                     DALVLTASLTYVDLKHMLYTLVASFVFSQMISVVQNGGYTIRGMIIITKHSEAAAQAI
                     LTEINRGVTYLKGQGAYSGNDYNIMYVTLNPTEVREVKRILAGLDPDAFISIIDVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(13077..13385)
                     /locus_tag="VHD02_000017"
     CDS             complement(13077..13385)
                     /locus_tag="VHD02_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEQ5496659.1"
                     /translation="MPSEKEILDALSKVYSEQVIQADDYFRQAIFELASQLEKEGMSS
                     LLATKIDSLINQYILTHQFDAPKSIFDLSRLVKTKASHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(13473..15164)
                     /locus_tag="VHD02_000018"
     CDS             complement(13473..15164)
                     /locus_tag="VHD02_000018"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEQ5496660.1"
                     /translation="MDTKTLIASEIAKVVPELEQDAIFNLLETPKNSDMGDLAFPAFS
                     LAKVLRKAPQMIASELAEQIDESQFEKVVAVGPYINFFLDKAKISSQVLEQVITAGSD
                     YAQQDEGQGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLAHIFAKMGYKPVKINHLGD
                     WGKQFGMLIVAYKKWGDEAAVQAHPIDELLKLYVRINAEAETDPTVDEEAREWFRKLE
                     DGDKEATELWQWFRDESLLEFNRLYDQLHVTFDSYNGEAFYNDKMDEVLDLLEAKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSVYVVGNE
                     QAAHFKQLKAVLKEMGYDWSDDMTHVAFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKEAVAHAVGVGAIKFYDLKTDRMNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKADFTPSATTTYSLADAESWEIIKLIQDFPRIIKRTSDNFEPSIMA
                     KFAINLAQSFNKYYAHTRILDDNSERDNRLALCYATATVLKEALRLLGVDAPNEM"
     gene            15455..15892
                     /gene="argR"
                     /locus_tag="VHD02_000019"
     CDS             15455..15892
                     /gene="argR"
                     /locus_tag="VHD02_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEQ5496661.1"
                     /translation="MNKMERQQQIKRIIQAEHIGTQEDIKNHLQKEGIVVTQATLSRD
                     LREIGLLKLRDEQGKLYYSLSEPVATPFSPEVRFYVLKVDRAGFMLVLHTNLGEADVL
                     ANLIDNDAIEDILGTIAGADTLLVICRDEEIAKRFEKDLAAGL"
     gene            16232..18787
                     /gene="mutS"
                     /locus_tag="VHD02_000020"
     CDS             16232..18787
                     /gene="mutS"
                     /locus_tag="VHD02_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEQ5496662.1"
                     /translation="MTKTNISPGMQQYLDIKKDYPDAFLLFRMGDFYELFYEDAVKAA
                     QLLEIGLTSRNKNAENPIPMAGVPHHSAQQYIDVLIELGYKVAVAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSAKPDSANNFLVAVDFDGCRYGLAYMDVSTGEFCVTDLADFTS
                     VRSEIQNLKAKEVLLGFDLSEEEQTILVKQMNLLLSYEETVYEDKSLIDGQLTTVELT
                     AAGKLLQYVHKTQMRELSHLQALVHYEIKDYLQMSYATKSSLDLVENARTNKKHGSLY
                     WLLDETKTAMGMRLLRSWIDRPLVSKEAILERQEIIQVFLNAFIERTDLSNSLKGVYD
                     IERLSSRVSFGKANPKDLLQLGYTLAQVPYIKAILESFDSPCVDKLVNDIDSLPELEY
                     LIRTAIDPDAPATISEGSIIRTGFDERLDHYRKVMREGTGWIADIEAKERQESGINNL
                     KIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGQMLEAREESS
                     SLEYDIFMCIRAQVETYINRLQKLAKTLATVDVLQSLAVVAETNHYIRPQFNDNHVIT
                     IQEGRHAVVEKVMGVQEYIPNSISFDQQTSIQLITGPNMSGKSTYMRQLALTVIMAQM
                     GSFVAADHVDLPLFDAIFTRIGAADDLISGQSTFMVEMMEANQAIKRASDNSLILFDE
                     LGRGTATYDGMALAQAIIEYIHDRVGAKTIFATHYHELTDLSTKLTSLVNVHVATLEK
                     DGDVTFLHKIAEGPADKSYGIHVAKIAGLPKSLLKRADEVLTRLETQSRSTEIMSVPP
                     QVESSSAVRQGQLSLFGDDEKAHEIRQALEAIDVMNMTPFQAMTTLYELKKLL"
     gene            18916..20898
                     /gene="mutL"
                     /locus_tag="VHD02_000021"
     CDS             18916..20898
                     /gene="mutL"
                     /locus_tag="VHD02_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEQ5496663.1"
                     /translation="MTNIIELPEVLANQIAAGEVVERPASVVKELVENAIDAKSSQIT
                     VEIEESGLKMIQVTDNGEGMSHEDLPLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     VASISKITIKTATKEVTHGSLLIATGGEIETLEAISTPTGTKIKVENLFYNTPARLKY
                     MKSLQAELAHIVDVVNRLSLAHPEVAFTLISDGRQLTQTSGTGDLRQAIAGIYGLNTT
                     KKMLVISNADLDFEVSGYVSLPELTRANRNYMTILVNGRYIKNFLLNRAILDGYGSKL
                     MVGRFPIVVIDIQIDPYLADVNVHPTKQEVRISKERELMALISTAISESLKEQDLIPD
                     ALENLAKSSTRHFSKPEQTQLPLQSRGLYYDPQKNDFFVKESAVSEKIPETDFYSGAV
                     DNSVKVEKAELLPHSEEVIGPSSVKHASRPQNTFTETDHPNLDLKNRQKLSQMLNRLE
                     NEEQSVFPELDYFGQMHGTYLFAQGKDGLFIIDQHAAQERVKYEYYRDKIGDVDSSLQ
                     QLLVPYLFEFSGSDFINLQEKMALLNEVGIFLEVYGHNTFILREHPIWMKEEEIASGV
                     YEMCDMLLLTNEVSIKTYRAELAIMMSCKRSIKANHSLDDYSARNLLLQLAQCQNPYN
                     CPHGRPVLINFSKADMEKMFRRIQENHTSLRELGKY"
     gene            20909..22129
                     /locus_tag="VHD02_000022"
     CDS             20909..22129
                     /locus_tag="VHD02_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ5496664.1"
                     /translation="MQEFLNLPKQIQLRQLVRFVTITLGSSIFPFMAMYYTTYFGTFW
                     TGLLMMITSLMGFVGTLYGGHLSDALGRKKVIMIGSVGTTLGWFLTILANLPNAAIPW
                     LTFAGILLVEIASSFYGPAYEAMLIDLTDESNRRFVYTINYWFINIAVMFGAGLSGLF
                     YDHHFLALLVALLLVNVLCFGVAYYYFDETRPETHAFDHGKGLLASFQNYRQVFQDRA
                     FVLFTLGAIFSGSIWMQMDNYVPVHLKLYFQPTAVLGFQVTSSKMLSLMVLTNTLLIV
                     LFMTVVNKLTEKWKLLPQLVVGSLLFTLGMLLAFTFTQFYAIWLSVVLLTFGEMINVP
                     ASQVLRADMMDHSQIGSYTGFVSMAQPLGAILASLLVSVSHFTGPLGVQCLFVVIALL
                     GIYFTVVSAKMKKV"
     gene            22131..22727
                     /gene="ruvA"
                     /locus_tag="VHD02_000023"
     CDS             22131..22727
                     /gene="ruvA"
                     /locus_tag="VHD02_000023"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEQ5496665.1"
                     /translation="MYDYIKGQLTKITAKYIVVETNGLGYIINVANPYSFTDSVNQLV
                     TIYLHQVIREDAHLLFGFHTEDEKDVFLKLISVSGIGPTTALAIVAVDDNEGLVNAID
                     NSDIKYLMKFPKIGKKTAQQMVLDLAGKFVEAPQETGNTKARSNKAGNTQLDEAIEAL
                     LALGYKATELKKIRAFFEGTSETAEQYIKSALKLLMKG"
     gene            22737..23297
                     /locus_tag="VHD02_000024"
     CDS             22737..23297
                     /locus_tag="VHD02_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEQ5496666.1"
                     /translation="MKRCSWVPKDNQLYCDYHDLEWGQPLDDDRDFFELLCLESYQSG
                     LSWLTVLKKRQAFRTVFHHYDIASVATFTSEEIADALENPSIIRHKLKLAATVNNAIA
                     VQKIQKEFGSFSTYLWNFVGGKPINNLVNQENLVPSQTELSIRLAKDLKKRGFKFLGP
                     TTVYSFMQASGLVNDHEEACVFNRIF"
     gene            23366..24637
                     /locus_tag="VHD02_000025"
     CDS             23366..24637
                     /locus_tag="VHD02_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEQ5496667.1"
                     /translation="MKAELIAVGTEILTGQIVNTNAQFLSEKMAELGIDVYFQTAVGD
                     NEERLLSVITTASQRSDLVILCGGLGPTKDDLTKQTLAKYLRKDLVYDEQACQKLDDF
                     FAKRKPSSRTPNNERQAQVIEGSIPLPNKTGLAVGGFITVDGISYVVLPGPPSELKSM
                     VNEELVPLLSKQYSTLYSKVLRFFGVGESQLVTVLSDFIENQTDPTIAPYAKTGEVTL
                     RLSTKTENQALADKKLGQLEAQLLSRKTLEGQPLADVFYGYGEDNSLARETFELLVKY
                     DKTITAAESLTAGLFQSTLASFPGASQVFNGGFVAYSMEEKAKMLGLPLEELKSHGVV
                     SAYTAEGMAEQARLLTGADIGVSLTGVAGPDMLEEQPAGTVFIGLATQNKVESIKVLI
                     SGRSRLDVRYIATLHAFNMVRKTLLKLENLL"
     gene            24726..25862
                     /gene="recA"
                     /locus_tag="VHD02_000026"
     CDS             24726..25862
                     /gene="recA"
                     /locus_tag="VHD02_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEQ5496668.1"
                     /translation="MAKKLKKNEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGTTQIKGTGDQKDSSIGKETKIKVVKNKVAPPFKVAE
                     VEIMYGEGISRTGELVKIASDLDIIQKAGAWFSYNGEKIGQGSENAKRYLAEHPELFD
                     EIDRKVRVKFGLLEESEEESAMAVASEETDDLALDLDNGIEIED"
     gene            26153..26551
                     /gene="spx"
                     /locus_tag="VHD02_000027"
     CDS             26153..26551
                     /gene="spx"
                     /locus_tag="VHD02_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEQ5496669.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHKLAYKEQNLGKEPLTKEEILA
                     ILSKTENGVESIVSSKNRYAKALDCDIEELSVSEVIDLIQDNPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNIENTEARLRAAL"
     gene            26664..26933
                     /locus_tag="VHD02_000028"
     CDS             26664..26933
                     /locus_tag="VHD02_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEQ5496670.1"
                     /translation="MGFTDETVRFKLDDGDKRQISETLTAVYHSLDEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDVK"
     gene            26930..27349
                     /gene="ruvX"
                     /locus_tag="VHD02_000029"
     CDS             26930..27349
                     /gene="ruvX"
                     /locus_tag="VHD02_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEQ5496671.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEEKAEFGFTR
                     LEELVKQYQVEQFVIGLPKNMNNTNGPRVDASITYGNHIEHLFGLPVHYQDERLTTVE
                     AERMLIEQADISRGKRKKVIDKLAAQLILQNYLNRNF"
     gene            27361..27666
                     /locus_tag="VHD02_000030"
     CDS             27361..27666
                     /locus_tag="VHD02_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEQ5496672.1"
                     /translation="MTHNHENDHQHEVITLVDEQGNETLFEILLTIDGREEFGKNYVL
                     LVPAGSEEDESGEIEIQAYSFTENEDGTEGDLQPIPEDSDAEWDMIEEVFNSFLDEN"
     gene            28079..29638
                     /locus_tag="VHD02_000031"
     CDS             28079..29638
                     /locus_tag="VHD02_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEQ5496673.1"
                     /translation="MIELNDEFIRKETIELANDGPRVHTTQYETKVPRLHKCYLLFFS
                     IIISSLTIAVPFLTDAANGLQSQNLYIGMMLTKGQLPYSDTFTTGGLFYFVIIALSYY
                     LGSTLWLVFVQVFCFYLSGLYLYKLINYMTGFQKVALTFSISYYLLSVSLGFGGLYPT
                     QLAMPFILISAWFLTKYFACLVKDEAFILFGFVGALAMLIDPSTLIFWSFACVTVFSY
                     NISQKHLARGFYQLLASIFGMILVFYTAGYFILNLQVLNPYLSQTMIYPFTFFKSGNL
                     SLLFGLAIQLFFALGLGLLTGMENVIRRFKNNSDRVVKWLFVMVILESILVAIFSQDY
                     RPYHLLPLLPFGLILTAIPVGYQYGIGLGQSSHRRRHGKNGVGRVMMIYLKRHFYLPI
                     LIVGTILICSTYCFISSIPLNQERDHIASYLEQKLNKTQSIYVWDDTSKIYLDSKAKS
                     VSQFSSPDINTQKVSHRKILEDELLENKAAYIVVNRYKNLPKIIQKVLSTNYKVDKQI
                     TTKSFIVYQKK"
     gene            29735..31933
                     /gene="nrdD"
                     /locus_tag="VHD02_000032"
     CDS             29735..31933
                     /gene="nrdD"
                     /locus_tag="VHD02_000032"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEQ5496674.1"
                     /translation="MVSLEEDKVTVQPDIKVIKRDGRLVNFDSTKIYSALLKASMKVT
                     RMSPLVEAKLEAISDRIIAEIIERFPTNIKIYEIQNIVEHKLLAANEYTIAKEYINYR
                     TQRDFARSQATDINFSIDKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAELNFKKHMADAKKWIVETKRESYAF
                     EKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSWFEREIQKAILTIRINGL
                     GSEHRTAIFPKLIFTVKRGLNLEPDSPNYDIKTLALECATKRMYPDMLSYDKIIDLTG
                     SFKSPMGCRSFLQGWKDENGQDVTSGRMNLGVVTLNLPRIAMESNGDMDKFWELFNER
                     MLISKDALIYRVERVTEAKPANAPILYQYGAFGKRLEKTGNVNDLFKNRRATVSLGYI
                     GLYEVASVFYGGQWEGNPDAKAFTLSIVKAMKQACENWSDEYGYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKSPTPFEKLDFEKDYPEAGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYNCQFEGDFTPTERGFTCPNCG
                     NNDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSTIKYPGL"
     gene            32115..32261
                     /locus_tag="VHD02_000033"
     CDS             32115..32261
                     /locus_tag="VHD02_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496675.1"
                     /translation="MGKYQLDYKGMQQVERFHEKHSKKKTDKKSRVQELKARFLEKSK
                     KQDN"
     gene            32290..33225
                     /locus_tag="VHD02_000034"
     CDS             32290..33225
                     /locus_tag="VHD02_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEQ5496676.1"
                     /translation="MLNIGIVGLGAISQKAYLPYMRQLSDITWHLSTRNAAVRQQVGQ
                     LFGHAILYSDVKELSKTNLDGVFIHAATKAHVDLASLFLRQGIPVFMDKPLADNYDSV
                     KYLYELAEAHHTFLMAGFNRRFAPCVKELSSLSTKRKVAAEKNDLNRPGDMTFKLFDF
                     FIHPLDTALFLTEGTLLKGHFQYHLEAGLLSQVMVTLMTESMTTTASMNLQSGSRREV
                     MEVQRAEETYHLENLDELSIYKGTEKRVLGFASWDTTLHKRGFETMIDAFLEAISTGV
                     NPVSPESSLLSHWICQQIADSQLSYGELTVELPKD"
     gene            33234..33743
                     /locus_tag="VHD02_000035"
     CDS             33234..33743
                     /locus_tag="VHD02_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ5496677.1"
                     /translation="MEIRRPTLKDKDAVLSMINEFLEQKSATDGLWHFNVNDFNYETW
                     LEDSLRQEMGLASQGVPAIQYVAFDEKNQALGFLNLRLRLNASLLEKGGHIGYSVRPS
                     QRGKGYAKEMLKQAVSCAISKNITTILVTCDETNVASRAVIVANGGVLEDSRGGTERY
                     WIEEQRGDK"
     gene            33743..34357
                     /gene="nrdG"
                     /locus_tag="VHD02_000036"
     CDS             33743..34357
                     /gene="nrdG"
                     /locus_tag="VHD02_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEQ5496678.1"
                     /translation="MAEKCWNNPKPKEWQAEELSQGRIIDYKAFNFVDGEGVRNSLYV
                     SGCLFHCKGCYNAATWSFKAGMPYTQELEEQIMTDLAQPYVQGLTLLGGEPFLNTGIL
                     IPLIKRIRCELPEKDIWSWTGYTWEEMMLETPDKLEMLSLIDILVDGRFDITKKNLML
                     QFRGSSNQRIIDVQKSLAAKEVIIWDKLNDGDQTFEQISREDLF"
     gene            complement(34531..35262)
                     /gene="yaaA"
                     /locus_tag="VHD02_000037"
     CDS             complement(34531..35262)
                     /gene="yaaA"
                     /locus_tag="VHD02_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEQ5496679.1"
                     /translation="MLTFLIPTAKEMVIPKESYPHLLPQPSQAILKAMAAMTTEDLAK
                     SYRIKEEAAKKEQQRWQDMASQQSLAYPAYQLFNGLMYRHIKRDKLTTQEQAYLTQQV
                     YITSSFYGIIPANHPIAEHRHDFHTRIKIEGQSLKSYWRPCYNQFAKEHPQVISLLSS
                     EFDDVFSKDCKQLWISPKFMAEKEGRFKTHSTISKKARGAFLTACMENNCQTVDSLKS
                     LVFAGFYYHPDLSTDHEFVYIKKEA"
     gene            complement(35397..36152)
                     /locus_tag="VHD02_000038"
     CDS             complement(35397..36152)
                     /locus_tag="VHD02_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEQ5496680.1"
                     /translation="MTYPYNSTISLGKVSLNVTDLAKMTTFYTSIIGLQVLSQDTTSR
                     QLTTDGKTVILELRQTPLPANKAYGLYHTAFLVPDRHSLGLHFLTRGISLEGAADHGY
                     SEAIYLSDPEGNGIEIYHDKAVEQWDIRDNGQIIGVTEPTDTKSILEQLTDIPKHFLL
                     AQDTRISHVHLSVKNALDSSLLYQKVFDLGDKMTIPSASWIASGNYHHHLAFNHWSAP
                     YLKKHQEGTPGLAFLTIHIETPLLFSATLKKAR"
     gene            36338..36676
                     /locus_tag="VHD02_000039"
     CDS             36338..36676
                     /locus_tag="VHD02_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ5496681.1"
                     /translation="MLKKRPACPVETTLSVIGNKWKLLILRDLLKGTLRFGQLKSSIG
                     SVSQKVLTAQLRAMEADGLVHRELYAEVPPRVEYSLTETGLSLAPVIEAMSDWDQTYQ
                     EKHADLFEEG"
     gene            complement(36955..37668)
                     /gene="treR"
                     /locus_tag="VHD02_000040"
     CDS             complement(36955..37668)
                     /gene="treR"
                     /locus_tag="VHD02_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEQ5496682.1"
                     /translation="MTKYERIYKDLETKINKDFYKEGDFLPTEIELSQQYQASRDTVR
                     KALSLLTKAGLILKKQGRGTQVIKHHQIMFPISELTSYQELVSYSNLDSKTNVIAIDK
                     LIVDETLSKLTGFSKNSLVWRVTRQRVVEGVASVLDIDYLSKTLVPIMTREIAEHSIY
                     QYLEKELHLAIDFALKEVTIDQITDRDKILLDLGSDQHVVSVKSKVYLSNNNQFQFTE
                     SRHKLEKFKFLDFSRRRPK"
     gene            37879..39903
                     /gene="treP"
                     /locus_tag="VHD02_000041"
     CDS             37879..39903
                     /gene="treP"
                     /locus_tag="VHD02_000041"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEQ5496683.1"
                     /translation="MGKFEQDAKSLLTAIGGKENIKAVTHCATRMRFVLNDNNKANVK
                     EIEKISVVKGTFTNAGQFQVIIGNDVPVFYNDFTAVSGIEGVSKEAAKSAAKSNQNAL
                     QRVMTMLAEIFTPIIPAIIVGGLILGFRNILESVPFEFLGQQVEKGKLVFDAAGDPVW
                     NTIVRVSPFWSGVNHFLWLPGEAIFHFLPVGITWSVTRKMGTTQILGIVLGICLVSPQ
                     LLNAYAVAGTPAAEIAKNWVWDFGFFTINRIGYQAQVIPALLAGLSLAYLEIFWRKRI
                     PEVVSMIFVPFLSLIPALILAHTVLGPIGWIIGKGISFVVLAGLTGPVKWLFGAIFGA
                     LYAPLVITGLHHMTNAIDTQLIADTATRTTGLWPMIALSNIAQGSAVFAYYLMNRHEE
                     REAEISLPAAISAYLGVTEPALFGVNVKYVYPFVAGMIGSGIAGLLSTTFDVQANSIG
                     VGGLPGFMAINVKYMIPFFICMAVAIVVPMFLTFFFRKSHIMTKTEDEAKLAETPVSE
                     APAATAPHKTMQGTVITLTSPLTGEVKALSEAVDPVFAQGVMGQGVLLQPTEGVLVAP
                     CDAEVSVLFPTKHAIGLVTAEGLELLMHIGMDTVNLDGQGFEALVKQGDQVKAGQTLI
                     QFDIAAISEAGYATEMPLVVTNQDTFTVTVEGSLPHQIKANDKLVVAVKK"
     gene            39970..41598
                     /gene="treC"
                     /locus_tag="VHD02_000042"
     CDS             39970..41598
                     /gene="treC"
                     /locus_tag="VHD02_000042"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEQ5496684.1"
                     /translation="MTIDKKKVVYQIYPKSYKDTTGNGVGDLRGIIDKLPYLQELGID
                     MIWLNPFYPSPQRDNGYDVSDYTAVNPDFGTMADFEDLVKAAKEHQIELMLDMVLNHC
                     STDHEWFQKALAGDPYYQDFFILRDQPTDWVSKFGGNAWAPFGDTGKYYLHLFDVTQA
                     DLNWRNPHVREELAKVVNFWRDKGVKGFRFDVINLIGKDEELVDCPVNDGKPAYTDRP
                     ITHTYLHDLNQASFGQDDSFMTVGEMSATTIDNCLLYTAPEREELSMAFNFHHLKVDY
                     ENGQKWTIMAFDFAALRDLFHAWGEGMSQGNGWNALFYNNHDQPRALNRFVDVTHFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDFDSMDNYVDVESLNAYSSLLVSGKSAE
                     EAFAIIKAKSRDNARTPMQWDASEHAGFTTGKPWLEVGKSYRDINVETEKEGRIFPFY
                     QRLIALRKELPIIAEGDYRAAFKDSQAVYAFERHLGDQCLLVLNHFYADEVELELPPR
                     YQHGQVLISNYEKVSICEKVILKPYQTLAILADN"
     gene            41806..43701
                     /gene="pepO"
                     /locus_tag="VHD02_000043"
     CDS             41806..43701
                     /gene="pepO"
                     /locus_tag="VHD02_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEQ5496685.1"
                     /translation="MTTYQDDFYQAVNGKWAETAVIPDDKPRTGGFSDLADEIEALML
                     DTTDAWLAGENIPDDAILANFVKFHRLVADYAKRDEVGVSPILPLIEEYQSLKSFSEF
                     VANIAKYELAGLPNEFPFSVAPDFMNAQLNVLWAEAPSILLPDTTYYEEGNEKAEELR
                     GIWRQSQEKLLPQFGFSTEEIKDLLDKVIELDKQLAKYVLSREEGSEYAKLYHPYAWA
                     DFTKLAPELPLDSIFEKILGQVPDKVIVPEERFWTEFAATYYSEANWDLLKANLIVDA
                     ANAYNAYLTDEIRVQSGTYARALSGTPQAMDKQKAAFYLAQGPFNQALGLWYAGQKFS
                     PEAKADVESKVARMIEVYKSRLETADWLAPATREKAITKLNVITPHIGYPEKLPETYA
                     KKVIDETLSLVENAQNLAKITIAHTWSKWNKPVDRSEWHMPAHLVNAYYDPQQNQIVF
                     PAAILQAPFYSLDQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNDWWTQEDYA
                     AFKERTDKIVAQFDGLESHGAKVNGKLTVSENVADLGGVACALEAAQSEEDFSARDFF
                     INFATIWRMKAREEYMQMLASIDVHAPGELRTNITLTNFDAFHETFDIKEGDAMWRAP
                     KDRVIIW"
     gene            complement(43868..44908)
                     /locus_tag="VHD02_000044"
     CDS             complement(43868..44908)
                     /locus_tag="VHD02_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEQ5496686.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDMDKAVELLREK
                     GMAKAAKKADRVAAEGLTGVYVHGNVAAVVEVNAETDFVAKNAQFVELVNATAKVIAE
                     GKPANNDEALALVMPSGETLAEAYVNATATIGEKISFRRFALIEKTDEQHFGAYQHNG
                     GRIGVISVVEGGDDALAKQVSMHIAAMKPTVLSYTELDAQFIKDELAQLNHAIELDNE
                     SRAMVDKPALPFLKYGSKAQLSDDVITAAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLDSVNAKAIAFARFEVGEGIEKKANDFES
                     EVAATMAAALNN"
     gene            complement(45042..45809)
                     /gene="rpsB"
                     /locus_tag="VHD02_000045"
     CDS             complement(45042..45809)
                     /gene="rpsB"
                     /locus_tag="VHD02_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEQ5496687.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVADEATRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFDVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDIIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDADVAFEADTQADSIEEIVEVVEGDNA"
     gene            46113..49187
                     /locus_tag="VHD02_000046"
     CDS             46113..49187
                     /locus_tag="VHD02_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ5496688.1"
                     /translation="MTKGIRFQLLGSPHIFLDDKEQFFAFAKANALLYYLVVNGSVSR
                     EVAASLLWENKNMQTAKKNLRNAIYQVNKVLQADVIICPNRNLLVLNKTLDIKTDINL
                     FLADPLAHLELYQGEFLQGFYLKSGEEFDLWVSKMRMQYEQVYLKACYQKIEEKLSLD
                     AIEDVEEHLKQLIERDEFEEKNYQLLMRLYQQGNCPGKVIETYYQLANVLDKELGIQP
                     SLQSQQIYQEVVAKDRNERKIKHFLRNSNHFLGRIDEIKQLENYFANCLACQEVGALL
                     LIGDTGIGKRTLARQVLANQTQTFQIVTAKCFREEAMDSLLPWRNILDGLGDLVIQNR
                     LLTTKAWKAALKRCFPVATIFQEDNNQPFIKDHTSLLVSFIVDILQHLAEIKALVILI
                     EDCHWMDEDSLTLLQRVMNQLVHYPIAFVLTKHLGTTPELGLCLNALMSQGRLESICL
                     EPFNRQESLAYINSQLGSQPVTAEEMEHLYQASQGNPFFLSEYTQALLRHEKFVPLTP
                     AIKAKLGLKLANLSSRDDALLNYLSCCRRPIPLNTLAQLMLLPLEEVIEMVDNLGHYY
                     ILVEESVGEEVLISFRQRIIQLYSYDRLSLSKRRLLHGQIAKRLEDLLPILTPSPHLL
                     DDIAYHYQESRQVIKALEYNLNYLDATLPFQHELFPIYSKSIGSLEKSDRDHQRLMEE
                     QFDKIRQSIADLELTYDNNRDFQQLLIRFSYLEGRYDIRTGRYQEGIKHIQKVIALAT
                     ELKQPSFLLEGYRQLIHYCIQVENKPEMRYYTSLSLEAAVAANHFEAIAISLRLNGLY
                     HLIIGELNEAERLLQQSIDFFKVTPGLQANYAIQIAAALDYLGEIAQIRYQFEKAVTY
                     QKQAIALTENKPAELSVSIFYIGLGISYFYLADFEQAEQILSLAKEALVNHSYPWKET
                     QLEIYLAMIQWKKGNYQPALTLLDYRETLMSRYRNPRDKGLVFYLMAVVKYQLIFQGA
                     TLSQQEKEMADHLLSESFEYYYEIASTNLNPYRDCHLVSELNDLRQQLSAKS"
     gene            complement(49218..50204)
                     /locus_tag="VHD02_000047"
     CDS             complement(49218..50204)
                     /locus_tag="VHD02_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HEQ5496689.1"
                     /translation="MLEDYYPSTTSYYHGGIDDDLYTAKWGMVMTFLDLNDSSLTPFE
                     GTHFALIGFKSDKGVYINNGRVGAVESPAAIRTQLAKFPWHLGNQVMVYDVGNIDGPN
                     RSLEQLQNSLSKAIKRMCDLNLKPIVLGGGHETAYGHYLGLRQSLSPSDDLAVINMDA
                     HFDLRPYDQTGPNSGTGFRQMFDDAVADKRLFKYFVLGIQEHNNNLFLFDFVAKSKGI
                     QFLTGQDIYQMGHQKVCRAIDRFLEGQERVYLTIDMDCFSVGAAPGVSAIQSLGVDPN
                     LAVLVLQHIAASRKLVGFDVVEVSPPHDIDNHTANLAATFIFYLVQIMAQHS"
     gene            complement(50390..51931)
                     /gene="hutH"
                     /locus_tag="VHD02_000048"
     CDS             complement(50390..51931)
                     /gene="hutH"
                     /locus_tag="VHD02_000048"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEQ5496690.1"
                     /translation="MTRVINLDGESLTIEDVIAIARQGVACRIDDSAIEAVNASRKIV
                     DDIVSEKRVVYGVTTGFGSLCNVSISPEDTVQLQENLIRTHASGFGDPLPEDAVRAIM
                     LIRINSLVKGYSGIRLSTIEKLLELLNKGVHPYIPEKGSLGASGDLAPLAHMVLPMLG
                     LGKAYYKGELLSGQEALDKAGIDKISLAAKEGLALINGTTVLTAIGALATYDAIQLLK
                     LSDLAGALSLEVHNGITSPFEENLHTIRPQSGQLATARNIRNLLEGSQNTTVATQSRV
                     QDPYTLRCIPQIHGASKDSIAYVKSKVDIEINSVTDNPIICKDGHVISGGNFHGEPMA
                     QPFDFLGIAISEIGNVSERRVERLVNSQLSKLPSFLVKYPGLNSGFMITQYACASLAS
                     ENKVLAHPASVDSIPSCENQEDFVSMGTTAARKAFEILKNSRRIVATEIMAACQALDL
                     KSENHELGKGTKVAYDLFRKEVNFIEHDKHIEIYDELNKASTVIEDPSFLEAVEQTVE
                     LSIQF"
     gene            complement(51942..53294)
                     /locus_tag="VHD02_000049"
     CDS             complement(51942..53294)
                     /locus_tag="VHD02_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEQ5496691.1"
                     /translation="MASNHMNEQEREQAKFSLSGATLYGINAVIGSGIFLLPRAIYKG
                     LGPASIAVMFGTAILTIMLAVCFAEVSGYFGKNGGAFQYSKRAFGDFIGFNVGFLGWT
                     VTIFAWAAMAAGFARMFIITFPAFEGWHIPLSIGLIILLSLMNIAGLKTSKIVTITAT
                     IAKLIPIVAFCVCTLFFIKNGLPNFTPFVQLEPGTNLLGAISNTAVYIFYGFIGFETL
                     SIVAGEMRDPEKNVPRALLGSISIVSVLYMLIIGGTIAMLGSQIMMTNAPVQDAFVKM
                     IGPAGAWMVSIGALISITGLNMGESIMVPRYGAAIADEGLLPAAIAKQNQNGAPLVAI
                     LVSGAIAIILLLTGSFENLAKLSVVFRFFQYIPTALAVMKLRKDDPDANVIFRVPFGP
                     IIPILAVIVSLVMIWGDNPMNFVYGAVGVIIASSVYYLMHGRNHQMDQMDQMPPKEQI
                     "
     gene            complement(53514..54110)
                     /locus_tag="VHD02_000050"
     CDS             complement(53514..54110)
                     /locus_tag="VHD02_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEQ5496692.1"
                     /translation="MTTVMVKTPADYTQTDWSGGETNQLFLYPEDGDYKKREFSYRLS
                     KSTVALRQTTFTLLDGYHRILMTLDKPITLSDLSHAKEISLTPFEPYAFEGDTRIVSR
                     GICQDINLMYDDQYQGQLLPVWSDTLFETPKADCQLLYALSKLTYSVTNQANQELEAN
                     HLLVIEQEASLSDLKVCPNQTSPAQTPVAVWAGLWYKT"
     gene            complement(54132..55805)
                     /locus_tag="VHD02_000051"
     CDS             complement(54132..55805)
                     /locus_tag="VHD02_000051"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEQ5496693.1"
                     /translation="MVLSDIEIANSVTMEPISKVADQLGIDKEALCLYGKYKAKIDAR
                     QLVALKNKPDGKLILVTAISPTPAGEGKTTTSVGLVDALSAIGKKAVIALREPSLGPV
                     FGVKGGAAGGGHAQVVPMEDINLHFTGDFHAIGVANNLLAALIDNHIHHGNSLGIDSR
                     RITWKRVVDMNDRQLRHIVDGLQGKVNGVPREDGYDITVASEIMAILCLSENISDLKA
                     HLEKIIIGYNFQGEPVTAKDLKAGGALAALLKDAIHPNLVQTLEHTPALIHGGPFANI
                     AHGCNSVLATKLALKYGDYAVTEAGFGADLGAEKFIDIKCRMSGLRPAAVVLVATIRA
                     LKMHGGVPKADLATENVQAVVDGLPNLDKHLANIQDVYGLPVVVAINKFPLDTDAELQ
                     AVYDACDKRGVDVVISDVWANGGAGGRELAEKVVALAEQDNQFRFVYNEDDSIETKLT
                     KIVTKVYGGKGIKLTPTAKRELAELERLGFGNYPICMAKTQYSFSDDAKKLGAPTDFI
                     VTISNLKVSAGAGFIVALTGAIMTMPGLPKVPASETIDIDEEGNITGLF"
     gene            complement(55823..56449)
                     /locus_tag="VHD02_000052"
     CDS             complement(55823..56449)
                     /locus_tag="VHD02_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEQ5496694.1"
                     /translation="MGLVDLSLTDFAKVLGSDAPAPGGGSAAALSGANGISLTKMVCE
                     LTLGKKKYADYQDIIEDLHTKSSRLQQDLLDAIDKDTEAFNLVSDVFDMPKETDEDKA
                     ARRTAMQKALKTAAQSPFEMMTLMVEALEITATAVGKSNTNAASDLGVAALNLKAGLQ
                     GAWLNVLINLSGIKDEDFVTDYRQKGQALLDKGCHLADDIYTKILDIV"
     gene            complement(56460..57359)
                     /gene="ftcD"
                     /locus_tag="VHD02_000053"
     CDS             complement(56460..57359)
                     /gene="ftcD"
                     /locus_tag="VHD02_000053"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEQ5496695.1"
                     /translation="MAKIVECIPNFSEGQNQAVIDGLVATAKSIPGVTLLDYSSDASH
                     NRSVFTLVGDDQSIQEAAFQLVKYASENIDMTKHHGEHPRMGATDVCPFVPIKDITTQ
                     ECVEISKQVAERINRELGIPIFLYEDSATRPERQNLAKVRKGQFEGMPEKLLEEDWAP
                     DYGDRKIHPTAGITAVGARMPLVAFNVNLDTDNIDIAHKIAKIIRGSGGGYKYCKAIG
                     VMLEDRHIAQVSMNMVNFEKCSLYRTFETIKFEARRYGVNVIGSEIIGLAPAKALIDV
                     AEYYLQVEDFDYHKQILENHLLG"
     gene            complement(57447..59477)
                     /locus_tag="VHD02_000054"
     CDS             complement(57447..59477)
                     /locus_tag="VHD02_000054"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEQ5496696.1"
                     /translation="MSFYSETDIAAAMTVKLDDVLPEKTVFKEGIRRSPDRGFRLTQA
                     QTEIALKNALRYVPTKFHEEVIPEFLEELKTRGRIYGYRWRPKDRIYGKPIDEYKGNC
                     TAAKAMQVMIDNNLSFEIALYPYELVTYGETGSVCANWMQYCLIKKYLEVMTDEQTLV
                     VESGHPVGLFKSKPEAPRVIITNGLLVGEYDNMKDWEIAEEMGVTNYGQMTAGGWMYI
                     GPQGIVHGTFNTLLNAGRLKLGVADDGDLTGKLFISSGLGGMSGAQGKAAEIAKSVAI
                     IAEVDQSRIETRHSQGWVSQIAESPEEALQLARKAIDAKESTSIAYHGNIVDLLEYVN
                     DKQIHVDLLSDQTSCHNVYDGGYCPVGISFDERTRLLAEDKDTFHQMVDDTLARHFEA
                     IKTLTENGTYFFDYGNAFMKSVYDSGITEISKNGRNDKDGFIWPSYVEDIMGPMLFDY
                     GYGPFRWVCLSGKHEDLVATDKAAMEAIDPDRRYQDRDNYNWIRDAEKNQLVVGTQAR
                     ILYQDCIGRVTIALKFNELVREGKIGPVMIGRDHHDVSGTDSPFRETSNIKDGSNVTC
                     DMAVQCYAGNAARGMSLVALHNGGGTGIGKAINGGFGLVLDGSERIDEIIKSAIAWDT
                     MGGVARRNWARNEHAIETAIEYNRLHAGTDHITIPYLADDDLVTSAVDQLFH"
     gene            59768..61033
                     /locus_tag="VHD02_000055"
     CDS             59768..61033
                     /locus_tag="VHD02_000055"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEQ5496697.1"
                     /translation="MVADVLLTHFNQLFCLNDPGHPLTGQEMKKATIVEDGYIAIKDG
                     LIVALGSGEPDAELVGPQTIMRSYKGKIATPGIIDCHTHLVYGGSREHEFAKKLAGVS
                     YLDILAQGGGILSTVRATRSASFDNLYQKSKRLLDYMLLHGVTTVEAKSGYGLDWETE
                     KRQLDVVAALEKDHPIDLVSTFMAAHAIPEEYKGNPKAYLDVIIKDMLPVVKEENLAE
                     FCDIFCEKNVFTADESRYLLSKAKEMGFKLRIHADEIASIGGVDVAAELSAVSAEHLM
                     MITNDDIAKLIGAGVIGNLLPATTFSLMEDTYAPARKMIDAGMAITLSTDSNPGSCPT
                     ANMQFVMQLGCFMLRLTPIEVLNAVTINAAYSVNRQERVGSLTVGKEADIAIFDAPNI
                     DYPFYFFATNLIHQVYKKGQLTVDRGRIL"
     gene            complement(61091..62623)
                     /gene="ahpF"
                     /locus_tag="VHD02_000056"
     CDS             complement(61091..62623)
                     /gene="ahpF"
                     /locus_tag="VHD02_000056"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEQ5496698.1"
                     /translation="MALSPDIKEQLAQYLTLLEADLVLQVSLGDNEQSQKVKDFVEEI
                     AAMSERISIENITLDRQPSFKVAKKGHDSGVVFAGLPLGHELTSFILALLQVSGRAPK
                     VDQDVIDRIKAIDRPLHFETYVSLTCHNCPDVVQALNIMSVLNDKISHTMVEGGMFQD
                     EVKAKGIMSVPTVFLDGEEFTSGRATIEQLLEQIAGPLSEEAFADKGLYDVLVIGGGP
                     AGNSAAIYAARKGLKTGLLAETFGGQVMETVGIENMIGTLYTEGPKLMAEVEAHTKSY
                     DVDIIKAQLATSIEKKENIEVTLANGAVLQAKTAILALGAKWRNINVPGEDEFRNKGV
                     TYCPHCDGPLFEGKDVAVIGGGNSGLEAALDLAGLAKHVYVLEFLPELKADKVLQDRA
                     ADTANMTIIKNVATKDIVGDDHVTGLNYTERDSGEDKHLDLEGVFVQIGLVPNTAWLK
                     DSGVNLTDRGEIIVDKHGSTNIPGIFAAGDCTDSAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(62644..63204)
                     /gene="ahpC"
                     /locus_tag="VHD02_000057"
     CDS             complement(62644..63204)
                     /gene="ahpC"
                     /locus_tag="VHD02_000057"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEQ5496699.1"
                     /translation="MSLIGKEIAEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLGVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQAFEVLGEDGLAQRGTFIVDPDGIIQMMEINADGIGRDASTLIDKIHAAQYVRKH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            63610..63680
                     /locus_tag="VHD02_000058"
     tRNA            63610..63680
                     /locus_tag="VHD02_000058"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63642..63644,aa:Cys,seq:gca)
     gene            64199..64402
                     /locus_tag="VHD02_000059"
     CDS             64199..64402
                     /locus_tag="VHD02_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEQ5496700.1"
                     /translation="MAQGTVKWFNAEKGFGFISTENGQDVFAHFSAIQTNGFKTLEEG
                     QKVAFDVEEGQRGPQAVNITKLA"
     gene            64598..65059
                     /locus_tag="VHD02_000060"
     CDS             64598..65059
                     /locus_tag="VHD02_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEQ5496701.1"
                     /translation="MPTKNTSDSIEEYIKELLAKSGIAEIKRSMLADSFQVVPSQINY
                     VIKTRFTESRGYEVESKRGGGGYIRIAKVHFSDKHHLIGNLMATIEDCISEQVFTDSI
                     QLLFDEHLLTEREGNIILAMASDDVLGTDGSTIRARMLYRLLQRIDRKGSN"
     gene            65059..67503
                     /locus_tag="VHD02_000061"
     CDS             65059..67503
                     /locus_tag="VHD02_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEQ5496702.1"
                     /translation="MIMYSTKMQDIFRQAQFQAARFDSHCLETWHVLLAMVAVDNSLA
                     NMILSEYDAQVAIEEYEAAAILAMGKTPKEQLSRVDFRPQSKTLTNLLAFAQAISQIT
                     RDQEVGSEHVLFAILLNPDIMASRLLEIAGYQIKDNGNGQPRLADLRKAIERHAGYSK
                     EMIKAIHELRKPKKTKTQGTFSDMMKPPSTAGELSDFTRDLTEMARQGLLESVIGRDQ
                     EVSRMIQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGAIPYELKEMRVLELDMM
                     SVVAGTRFRGDFEERMNQIIDDIEADGQIILFVDELHTIMGSGSGIDSTLDAANILKP
                     ALSRGTLHMVGATTQEEYQKHIEKDAALSRRFAKILIEEPNTEDAYQILMGLKLSYET
                     YHNVSISNEAVKTAVKMAHRYLTSKNLPDSAIDLLDEASAAVQNMVKKSAPETLTPID
                     QALINGDMKKVSRLLAKEAKGQMRKPTPVTEDDILATLSKLSGIPLEKLTQADSKKYL
                     NLEKELHKRVIGQDAAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKAL
                     AEVLFDDEAALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGELTQKVRNKPYSVLLF
                     DEVEKAHPDIFNVLLQVLDDGILTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFG
                     VKGIHQDHQAMEKRILEELRKTYRPEFINRIDEKVVFHSLTQDNMRDVVKIMVQPLIT
                     TLAEKGITLKIQPLALKHLSEVGYDEHMGARPLRRTLQTEIEDKLSELILSRELTSGH
                     TLKIGLSHGKLTFHIA"
     gene            67681..67971
                     /gene="groES"
                     /locus_tag="VHD02_000062"
     CDS             67681..67971
                     /gene="groES"
                     /locus_tag="VHD02_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEQ5496703.1"
                     /translation="MLKPLGDRVVVRFDDEKEQTVGGFVLAGTHKESTRKATVLAVSE
                     TGVRTITGDSVLPSVSVGQEVLVENGHDLEVTVDDEKVSIIRESDIIAIVTK"
     gene            68007..69638
                     /gene="groL"
                     /locus_tag="VHD02_000063"
     CDS             68007..69638
                     /gene="groL"
                     /locus_tag="VHD02_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880885.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEQ5496704.1"
                     /translation="MAKDIKFSADARAAMVRGVDMLADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVHEG
                     LKNVTAGANPIGIRRGIETATATAVEALKAIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVADLENPFI
                     LITDKKVSNIQDILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATMTALGQAAKITVDKDSTVIVEGS
                     GSSEAIANRIALIKSQLETTTSDFDREKLQERLAKLAGGVAVIKVGAPTETALKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALITVIEKVAALELEGDDATGRNIVLRALEEPVRQ
                     IALNAGYEGSVVIDKLKNSPAGTGFNAATGEWVDMIKTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPAAPAPAMPAGMDPGMMGGF"
     gene            69889..70155
                     /locus_tag="VHD02_000064"
     CDS             69889..70155
                     /locus_tag="VHD02_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ5496705.1"
                     /translation="MNHFGEILKTFRESKGLRLKDVAKAGISTSQLSRFEKGETDLTI
                     STFMLILDESNMPIDEFMYAVHDFHRDDLNELLSKSEAFRNNSR"
     gene            complement(70867..72366)
                     /locus_tag="VHD02_000065"
     CDS             complement(70867..72366)
                     /locus_tag="VHD02_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEQ5496706.1"
                     /translation="MINKKISLGVLSILTAFSLQSVSYACTGFIIGKDLTKDGSLLYG
                     RTEDLEPHHNKNFIVRLAKDNPAGEKWKDLSNGFEYPLPEHSYRYSAIPDVTPNKGVY
                     DEAGFNEFGVSMSATVSASANDAIQKIDPYVKNGLAESSMASVILPSVKIAREGVALI
                     AKIVTEKGAAEGNIVTLADKDGIWYMEILSGHQYVAIKFPDDKYAVFPNTFYLGHVDF
                     NDKENTIASEDVEKVAKKAKSYIEVDGKFHIAKSYNPPLNDANRSRSFSGIKSLDPDS
                     KVTYKDSNYELLQSTDKTFSLEDAMKLQRNRFEGLDLKPLDQMALDGKGKPKSKKAVK
                     GYAYPISNPNVMEAHIFQLKKDIPAELGGGVMWLSIGSPRNAPYLPYLGNISRTYEAY
                     QEKSTQYNDKSWYWTVSHINDLVAAHPKPFGTKVIDEMKGLEKTWIAEQDKSTKEISD
                     LVVSDPKAAQEKADKISLDRAEKTFKRLKAIEAKLVKEKPKNKKGLNRS"
     gene            72613..74253
                     /locus_tag="VHD02_000066"
     CDS             72613..74253
                     /locus_tag="VHD02_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496707.1"
                     /translation="MTKPFHHKKLKQITIIAATSLFLFLIGGAFYYSKNHCINAYLKA
                     RSAQSGPVFENIKAYLVWDDTNEQITNDEAMYTKFRRYSQKELRQKKQDLKAASQDSA
                     VQVKSVGRRFWIFPDYRIAIKPMDLTIKTNVPQADVLLNQKKVAVSDSEQFSVKLDRL
                     PTAEYTASIRGKHNGRNIKVNKSYDGDNPVLDLSVSFRTFLVTSNAKQGDLYFDDNHI
                     GTLKDGQLQVEDYPVTENAQAYMKTTFPDGELRSQKYALADVEEGATLEILVTDLLEE
                     DKAGELLVSAFDQLMHYLSTGQDSSNLRSVFEAGSSNAFYRGLKESIKAKFQTDTRKA
                     SRLNIPSILLTTMTQVGKTIYVLDFTATYEFLYDNSTDPEQHTSGHINQDLTGKVTVK
                     KVGQHYLISQSGSKNITVVKEDNQLKAPSVFPESILGTWTGQANGLSIHMSLASDGTI
                     TTKVEDQKGNRSKETRTAKISKVEDKGNGFYLYTPDPGSDISALAPEGGLGGANVKYA
                     YGFKISGKTASPVVWQAALTHEFDYTKPLSGVTLQKQP"
     gene            complement(74342..75343)
                     /locus_tag="VHD02_000067"
     CDS             complement(74342..75343)
                     /locus_tag="VHD02_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEQ5496708.1"
                     /translation="MTSVKVLKLSLLLLPPKWLSTLLKNTITGSMKTTNKPSKGFTVT
                     LKNPFEELTVKALLEEQLLIPRKIRHFLRTKKHVLINGHPVNWQSCVKYGDQVKLFFD
                     HEDYPEKIIVMGQAEKVTCLYEDEHIIIVNKPEGMKTHGNDPTELALLNHVSAYTGQT
                     CYVVHRLDKETSGAILFAKTPFILPILNRLLEKRDIHREYLALVHGSLDSPRVTYHHP
                     IGRHRHDRRKRVVDPINGKKAITEVTLVKNFHKTASLLNCQLQTGRTHQIRVHLAHQG
                     HVLFGDPLYSNGKKDCARLMLHAYQLRLKHPLTREDICVQAKSATFDAVLNAQKARK"
     gene            75919..76299
                     /locus_tag="VHD02_000068"
     CDS             75919..76299
                     /locus_tag="VHD02_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEQ5496709.1"
                     /translation="MKTIRRYDVNEDRGHTGLVEAGDFYYLNYCVGNVGQDIESQING
                     AFDEMERRLALVGLTLDAVVQMDCLFRDVWNIPVMEKMIKERFNGRYPARKSIQTEFA
                     HHGGPQGLLFQVDGVAYSKHISMT"
     gene            76338..78674
                     /locus_tag="VHD02_000069"
     CDS             76338..78674
                     /locus_tag="VHD02_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ5496710.1"
                     /translation="MRFLELLQKKFFPKAYQEKQFLMHQKTRLTPQHNQKQYSPNANH
                     LDSSATKNSEQDPAIALQRSRAYEGSPKSRPAWLQKLEAVLPSPQHPIRRFWRRYHIG
                     KLLMILIGTLVLLLGSYLFYLSKTAKVSDLQDALKATTVIYDHKGEYAGSLSGQKGSY
                     VELNAISDDLENAVIATEDRTFYSNSGINLKRFLLAVVTAGRFGGGSTITQQLAKNAY
                     LSQDQTIKRKAREFFLALELTKKYSKKDILTMYLNNSYFGNGVWGVEDASQKYFGTTA
                     ANLTLDEAATLAGMLKGPEIYNPYHSLKNATHRRDTVLGAMVDAKKITQTKAQQARAV
                     GLKNRLADTYVGKTDDYKYPSYFDAVISEAIATYGLSEKDIVNNGYKVYTELDQNYQT
                     GMQTTFNNDELFPVSAYDGSSAQAASVALDPKTGGVRGLIGRVNSSENPTFRSFNYAT
                     QAKRSPASTIKPLVVYAPAVASGWSIEKELPNTVQDFDGYQPHNYGNYESEDVPMYQA
                     LANSYNIPAVSTLNDIGIDKAFTYGKTFGLDMSSAKKELGVALGGSVTTNPLEMAQAY
                     AAFANNGVIHPAHLINRIENARGEVLKTFTDKAKRVVSQSVADKMTAMMLGTFSNGTA
                     VNANVYGYTLAGKTGTTETNFNPDLAGDQWVIGYTPDVVISQWVGFNQTDENHYLTDS
                     SAGTASAIFSTQASYILPYTKGSQFHVDNAYAQNGISAVYGVNETGNQSGVDTQSIID
                     GLRKSAQEASQSLSKAVDQSGLRDKAQSIWKEIVDYFR"
     gene            78723..78875
                     /gene="rpmG"
                     /locus_tag="VHD02_000070"
     CDS             78723..78875
                     /gene="rpmG"
                     /locus_tag="VHD02_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEQ5496711.1"
                     /translation="MAQKKASLACVECGSRNYSIGVSSTPKPTRLEVNKFCKYCKTYT
                     LHKETR"
     gene            78889..79065
                     /gene="secE"
                     /locus_tag="VHD02_000071"
     CDS             78889..79065
                     /gene="secE"
                     /locus_tag="VHD02_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEQ5496712.1"
                     /translation="MGFISGTFKVLKDTTWPNRKQRWKDFISVLEYTAFFTVIIYIFD
                     QLLAKSVLALINLF"
     gene            complement(79302..80075)
                     /locus_tag="VHD02_000072"
     CDS             complement(79302..80075)
                     /locus_tag="VHD02_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEQ5496713.1"
                     /translation="MTDRGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQQ
                     KVPEQMLTSDGLNTKIVGEEKTVDEVIEEVLKDLDFYEESGGGMTLSGGEIFAQFDFA
                     LALLKAAKAAGLHTAIETTAFAKHEQFVTLIDYVDFIYTDLKHYNQLRHQKVTGVRND
                     LIIKNIHYAFQAGKEIVLRIPVIPQFNDSLDDAKAFSELFNQLEIDQVQLLPFHQFGE
                     NKYKLLGREYEMAEVKAYHPEDLADYQAVFLNHNIHCYF"
     gene            80201..80947
                     /locus_tag="VHD02_000073"
     CDS             80201..80947
                     /locus_tag="VHD02_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ5496714.1"
                     /translation="MNRLERIIQLVSQKKKIDVNSLSEQLDVSKVTIRKDLDKLESKG
                     LLRREHGYAVLNSGDDLNVRLSYNYNIKRRIAEKAAELVQDNDTIMIESGSTCALLAE
                     VLCQTKRNIKVITNSCFIANYIRQYSSCQIILLGGYYQPNSEVTVGPLLKEMISLFHV
                     NRVFVGTDGFNKDLGFMGKDMMRSEGVRYMADAAEEVVILTDSSKFSKTSLVHQLSLA
                     DVNRVITDQALDKQTQELLSASGLVLDFVS"
     gene            80963..81943
                     /locus_tag="VHD02_000074"
     CDS             80963..81943
                     /locus_tag="VHD02_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEQ5496715.1"
                     /translation="MKEERRRLLAKVAYLHYVQGKSQTLISKEMNIYRTTVCRMLAKA
                     KEEGIVRIEIADYDADLFALEEYVRQQYGLEKLDLVPNQVEDTPMDTLTNVAKTAAEV
                     FRHVVKDGDKIGLSWGATLSCLMDELNPKAMKDVFIYPLAGGPSHINAKYHVNTLVYR
                     LARIFHGNSAFMNAMVIQEDKHLAKGILQSKYFNDILTSWDQLDLALVGIGGEPNSLE
                     QSQWRDLLTSSDHDQLKYEKAVGEVCCRFFDQAGQPVYTGLQDRTIGISLEQLRRVPK
                     TMAVATGKHKAKAILAALKAGFINYLVTDKETMLAVLALDEDIDLNNVLL"
     gene            82230..82550
                     /locus_tag="VHD02_000075"
     CDS             82230..82550
                     /locus_tag="VHD02_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEQ5496716.1"
                     /translation="MQVIVPDQIIMGLILNAGDAKQHIYQALKCAKEDDYATSEKEMA
                     LADDALLEAHNLQTQFLAQEASGNKSEITALFVHSQDHLMTTITEINLVKEIIDLRKE
                     LATK"
     gene            82578..82886
                     /locus_tag="VHD02_000076"
     CDS             82578..82886
                     /locus_tag="VHD02_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ5496717.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKVAAEKKGIDCQIEAYAQGKLAD
                     YAPLLDVALLGPQVAYTLDQSEAICKDNDIPIAVIPMADYGMLDGNKVLDLALSLVKE
                     "
     gene            82896..84200
                     /locus_tag="VHD02_000077"
     CDS             82896..84200
                     /locus_tag="VHD02_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEQ5496718.1"
                     /translation="MAKMNMQNIIMPIMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     AGQIPFQGINDAIAGVFGADWTEPFMQVYHGTFAIMGLISCFAIGYSYAKNSGVEPLP
                     SGVLSLSAFFILLRSSYVPAEGEPVGDAISKAWFGGQGIIGAIVIGLTVGAIYTAFIR
                     RHIVIKMPEQVPQAIAKQFEAMIPAFVIFTLSMLVYIIAKSVTGGGTFIEMIYDVIQV
                     PLQGLTGSLYGALGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKALMAAGELS
                     LDKGAHIVTQQFLDSFLILSGSGITFGLVVAMIFAAKSKQYKALGKVAAFPALFNVNE
                     PVVFGFPIVMNPVMFLPFILVPVLAALTVYGAIAIGFMQPFAGVTLPWSTPAIISGFM
                     VGGWQGAIVQILILIMSTLVYFPFFKIQDNMAYQNEQASEES"
     gene            84411..86828
                     /locus_tag="VHD02_000078"
     CDS             84411..86828
                     /locus_tag="VHD02_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEQ5496719.1"
                     /translation="MTETKSPYFGHLTDRMTHYREAVLDKKPYIDAERAILATEAYQK
                     HQNKPANLKRAYMLQTILENMTIYIEDESLIAGNQASSNKDAPIFPEYTLEFVLNELD
                     LFEKRDGDVFYITEETKQQLRDIAPFWENNNLRARCGVLLPEEVQVYMETGFFGMEGK
                     MNSGDAHLAVNYQKLLEHGLKGFEERARAAKAALDLTIPENIDKYHFYDSVFIVIDAV
                     KTYAKRYAKLARELAKTAKPERQAELLDIARICDKVPYEPAKTFAEAVQSVWFIQCIL
                     QIESNGHSLSYGRFDQYMYPYVKADLEAGRETEASIVERLTNLWIKTLTINKVRSQAH
                     TFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLRSVAQTKLPQPNLTVRYHKGLDNT
                     FMNECIEVMKLGFGMPAMNNDEIIIPSFIKKGVSEEDAYDYSAIGCVETAVPGKWGYR
                     CTGMSYINFPKILLITMNDGIDPASGKRFAKGHGHFKDMTSYEELKAAWDATLREITR
                     MSVIVENAIDLGLEREVPDILCSALTDDCIGRGKTLKEGGAVYDYISGLQVGIANLSD
                     SLAALKKLVFEEGQLTPEELWQALESDFAGERGEEIRQMLINDAPKYGNDDDYADSLV
                     VEAYDTYIDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGKGTLATPDGRHAGTPL
                     AEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQKVNPQTLAKEEDKLKLMALL
                     RTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQDDII
                     ERTEHTL"
     gene            86841..87509
                     /locus_tag="VHD02_000079"
     CDS             86841..87509
                     /locus_tag="VHD02_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEQ5496720.1"
                     /translation="MEYMLDTLDLEAIKKWHHILPLAGVTSNPSIAKKEGDIDFFERI
                     REVRAIIGDKASIHVQVIAQDYKGILKDAAEIRRQCGDSVYVKVPVTTEGLAAIKTLK
                     AEGYHITATAIYSTFQGLLAIEAGADYLAPYYNRMENLNIDPEAVIGQLAEAIDRECS
                     DSKLLAASFKNVAQVNKSFALGAQAITAGPDVFEAGFAMPSIQKAVDDFGKDWEAIHH
                     RKSI"
     gene            87566..88654
                     /locus_tag="VHD02_000080"
     CDS             87566..88654
                     /locus_tag="VHD02_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEQ5496721.1"
                     /translation="MKVFASPSRYIQGKNALFTNAKTLKQLGNSPILLCDDVVYGIVG
                     ERFESYLIDNGMTPVHVAFNGEASDNEISRVVAIAKENGNDVIIGLGGGKTIDSAKAI
                     ADLLAVPVIIAPTIASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVICQAP
                     KRLLASGIADGLATWVEARAVMQKNGDTMAGGNQTLAGVAIAKACEQTLFADGLKAMA
                     SCDRQVVTPALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALTGAIHHLTHGEKVA
                     YGTLTQLFLENRSREEIDRYIDFYQAIGMPTTLKEMHLDTATQEDFLKIGRQATMAGE
                     TIHQMPFVISPEDVAAALVAVDAYVTSR"
     gene            complement(88736..89245)
                     /locus_tag="VHD02_000081"
     CDS             complement(88736..89245)
                     /locus_tag="VHD02_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEQ5496722.1"
                     /translation="MSTDHHLREVSYQLLAERGGSLEAIAELVLFLQNDYIPNLTMAE
                     CLESVEAVLAKREVQNAIITGVELDKLAEANQLSEPLLSILKTDQGLYGIDEILALSI
                     VNLYGSIGFTNYGYLDKTKPGIVDKLNHKDGNSCHTFLDDIVSAIAAAAASRIAHNDP
                     AKSAITNQQ"
     gene            89609..90424
                     /gene="sdaB"
                     /locus_tag="VHD02_000082"
     CDS             89609..90424
                     /gene="sdaB"
                     /locus_tag="VHD02_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptodornase B"
                     /protein_id="HEQ5496723.1"
                     /translation="MNLLGSRRVFSKKCRLVKFSMVALVSATMAVTTVTLENTALARQ
                     TQVSNDVVLNDGASKYLNEALAWTFNDSPNYYKTLGTSQITPALFPKAGDILYSKLDE
                     LGRTRTARGTLTYANVEGSYGVRQSFGKNQNPAGWTGNPNHVKYKIEWLNGLSYVGDF
                     WNRSHLIADSLGGDALRVNAVTGTRTQNVGGRDQKGGMRYTEQRAQEWLEANRDGYLY
                     YEAAPIYNADELIPRAVVVSMQSSDNTINEKVLVYNTANGYTINYHNGTPTQK"
     gene            complement(90665..91507)
                     /gene="ropB"
                     /locus_tag="VHD02_000083"
                     /gene_synonym="rgg1"
     CDS             complement(90665..91507)
                     /gene="ropB"
                     /locus_tag="VHD02_000083"
                     /gene_synonym="rgg1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982409.1"
                     /note="RopB (Regulator of Protease B) is a paralog of the
                     quorum-sensing transcriptional regulators Rgg2 and Rgg3,
                     which bind short, hydrophobic peptides encoded by genes in
                     their respective vicinities. RopB binds the leaderless
                     short peptide SIP (SpeB-inducing peptide), with sequence
                     MWLLLLFL; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     RopB/Rgg1"
                     /protein_id="HEQ5496724.1"
                     /translation="MEIGETVEFIRHSKNISIKQVCGDYLTRQTYYRFIKNNLDISSK
                     KLLYILDNLNVNVDEFLFISNNFKQYKEFIDMDTAKHYFECRNIDGLNHILDSYKDSK
                     STKEKNLFALVKVLLATLTEEDCLTERTYLSNYLINIETWSHYETVLFNNCMFIFESC
                     FIEMVFSKVILNLDKYNTLRYYGNESIRMFVNMLILFIQRQEYDKASEILAKIEDYQL
                     NDDCLYERCCVSFFDGIIGLINGKEGAEQKCVQILEIFQLLNCKTIHHMFQTYLEAIK
                     HKLS"
     gene            92006..92176
                     /locus_tag="VHD02_000084"
     CDS             92006..92176
                     /locus_tag="VHD02_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496725.1"
                     /translation="MDNPKKDKGFSLGVVDPITILITESVINKMRTVPYHLKKLSSTT
                     YCLLADLAYQSA"
     gene            92448..93644
                     /gene="speB"
                     /locus_tag="VHD02_000085"
     CDS             92448..93644
                     /gene="speB"
                     /locus_tag="VHD02_000085"
                     /EC_number="3.4.22.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine proteinase exotoxin SpeB"
                     /protein_id="HEQ5496726.1"
                     /translation="MNKKKLGIRLLSLLALGGFVLANPVFADQNFARNEKEAKDSAIT
                     FIQKSAAIKAGARSAEDIKLDKVNLGGELSGSNMYVYNISTGGFVIVSGDKRSPEILG
                     YSTSGSFDANGKENIASFMESYVEQIKENKKLDTTYAGTAEIKQPVVKSLLDSKGIHY
                     NQGNPYNLLTPVIEKVKPGEQSFVGQHAATGCVATATAQIMKYHNYPNKGLKDYTYTL
                     SSNNPYFNHPKNLFAAISTRQYNWNNILPTYSGRESNVQKMAISELMADVGISVDMDY
                     GPSSGSAGSSRVQRALKENFGYNQSVHQINRSDFSKQDWEAQIDKELSQNQPVYYQGV
                     GKVGGHAFVIDGADGRNFYHVNWGWGGVSDGFFRLDALNPSALGTGGGAGGFNGYQSA
                     VVGIKP"
     gene            93646..93951
                     /locus_tag="VHD02_000086"
     CDS             93646..93951
                     /locus_tag="VHD02_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spi family protease inhibitor"
                     /protein_id="HEQ5496727.1"
                     /translation="MEMHFVRTEPEARRIAETFCAENTQTKTPMRVQQLSYPSDTDHS
                     GGELYIYALSPAGFIIVSGDTRAHTILGYSFDNNLDLNHDNVRSMVEAYQKQINSLD"
     gene            94431..95360
                     /gene="prsA"
                     /locus_tag="VHD02_000087"
     CDS             94431..95360
                     /gene="prsA"
                     /locus_tag="VHD02_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEQ5496728.1"
                     /translation="MKQMNKLITGVVTLATVVTLSACQSSHNNTKLVSMKGDTITVSD
                     FYNETKNTELAQKAMLSLVISRVFETQYANKVSDKEVEKAYKQTADQYGTSFKTVLAQ
                     SGLTPETYKKQIRLTKLVEYAVKEQAKNETISKKDYRQAYDAYTPTMTTEIMQFEKEE
                     DAKAALEAVKAEGADFAAIAKEKTIAADKKITYTFDSGETTLPAEVVRAASGLKEGNR
                     SEIITALDPATSKRTYHIIKVTKKATKKADWKAYQKRLKDIIVTGKLKDPDFQNKVIA
                     KALDKANVKIKDKAFANILAQFAKPNQKQPAQK"
     gene            complement(95631..95867)
                     /locus_tag="VHD02_000088"
     CDS             complement(95631..95867)
                     /locus_tag="VHD02_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496729.1"
                     /translation="MTHRFFLTPLSTLWRRLRPKRYSKKVLADEPTSYQPPTAHGPCD
                     DTHQLLEDKSLSDDYLITTSAMRRLWYWGYSQIG"
     gene            96290..99232
                     /locus_tag="VHD02_000089"
     CDS             96290..99232
                     /locus_tag="VHD02_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEQ5496730.1"
                     /translation="MTNCKYKLRKLSVGLVSVGTMLIAPTVLGQEVSNTEASASSTAS
                     VDATASGTTASGASSEATVATTNGGSQSTQVAAETTPQPQVQTSEQPAATSTSLASST
                     SSSEDKAPKAASTKSSSATVASSSNGSNQGAGTEVEPQMMDVEQYKVNKEKTELTVKD
                     DNQQLKIRKDVDELKNKDLFDVKREVKDNGDGTLDVTLKVTPKQIDEGADVMALLDVS
                     QKMTDADFNNAKEKIKKLVKTLTSKPTDSHPNHNARNSVRLMTFYREVSDPIDISGKT
                     DEELNKILDDVWQNAKHNWDWGVDLQGAIHKARGIFKQDQEKKSGKRQHIVLFSQGES
                     TFSYDIQKSEKERDNNLSRINEKITSSNPLLHWPPIFNHTHQNADMLNEIERIIKIGE
                     QLGIKGLSSIKGYLSTAAAGSGILGNFIGGGSLTEYLTLKEYNSDKLEESQFDYTKRV
                     GEGYYYYSFSKRVSHNDFPFKTLIEQAITQAIPTKGESKNWFEKFLDKFSLVESYRDT
                     QKKALLKILEYLFYKREYHYYNHNLSAIAEAKMAQQEGITFYSVDVTDSETTPKRVRR
                     QTAVRQEKAKKESKEERNEKFDKYLKKMSEGNTFLDSVEEKDKFKDTFTNVTVTETFE
                     DKVSAIANSWESSLDSHNNKSDRNHKGSVKHTQAQSSGWFANTQESLTWTISKDQLKK
                     AFEDGEPLTLTYKLKVDKDKFRETLKKQQERNRKKRAASPESENTVTEKIISNKISYK
                     INNGKDINNNKLEDVKMSYSKFKMPIPELDIEVVPIPEKPLVEPMTPLYPAIPNYPTP
                     QLPKDEDLEISGGHGPIVDIVEDTGTGVEGGSQNGVVSTQENKDPIVDITEDTQPGMS
                     GSNDATVVEEDTAPKRPDILVGGQSDPIDITEDTQPGMSGSNDATVIEEDTKPKRFFH
                     FDNEPQAPEKPKEQPSNSLPQAPVYKAAHHLPASGDKREASFTIVALTIIGAAGLLSK
                     KRRDTEEN"
     gene            99412..101379
                     /locus_tag="VHD02_000090"
     CDS             99412..101379
                     /locus_tag="VHD02_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEQ5496731.1"
                     /translation="MTKKFIKSAVILGLASITISMTQPVEAISNSLEYQAQYKRVKEE
                     LDALKEKGPKLGERSLYFKVVNRFLGWFGVVWDPLAIKETDHLLDLIGQVNYLVSNTE
                     FQAPEYQKAKNKLDLAVAGYDLYLAREKKRGDAPDYQVTQLTLGQLETIKTKIQEALK
                     TFAQEVKTIQQKKPDLFPHAYVLNAKSKDITDKAQPFLVNGYYDVFYKQFLFTNTNDG
                     YNYNKEADNLASYQKLIKELLGLKAEIDQLTKDDPKNDRLETLMWESKFTLSDSRRTS
                     RLSAYYTQEDYVKKVVLSEEAPNLRTAKEKVKTLKGILHDYYVDIKEPEKAKKYRVED
                     ETLPSQPEAPAKPEAPAPSPSPAPGQKPAEDERSSQATEPANPSKENSSTDASQGSHD
                     SENPATDSPSQPQASDQGNHQSQVPNAKPQTDKTDTPNAPVPPQNTPNAPAIPQDTPK
                     VPEAGGQSGPAGNAEEKAPDSGPKESGQSSSKESPSVGEDTTPNQPDVLVGGQREPID
                     ITEDTITDAPPTVSGHNASTQPQSVVEDTAPQRPDVLVGGQSEPIDITQDTQPGMSGS
                     NDATVINEDTKPKRVFHFDNKEPQASEKAAEQKLAPHDSHTTPQTSDDTAAPHLPASG
                     DKPEKVFALAALTMVAAAGLLSKKRRNTDKN"
     gene            101521..101817
                     /locus_tag="VHD02_000091"
     CDS             101521..101817
                     /locus_tag="VHD02_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEQ5496732.1"
                     /translation="MTKQDQLIVEKMEQTYEAFSPKLANLIEALDAFKEHYEEYATLR
                     NFYSSDEWFRLANQPWDDIPCGVLSEDLLFDMIGDHNQLLADILDLAPIMYKHM"
     gene            101965..102249
                     /locus_tag="VHD02_000092"
     CDS             101965..102249
                     /locus_tag="VHD02_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEQ5496733.1"
                     /translation="MKFKKVLVIPALALAATCFLTACGTKKDSKKEEVKEIKMSDIKD
                     DAVSKKTKVVDGEEVTEYTTKDGNVIQIPAGNEEGMESKDAGGSGAPAKN"
     gene            102673..103941
                     /locus_tag="VHD02_000093"
     CDS             102673..103941
                     /locus_tag="VHD02_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEQ5496734.1"
                     /translation="MFQLRKKMTRKQLALLSAGVLTCVVGGTYLIMNHQQQEIVSSVN
                     KVKALTIKEAMEQGKDISLTLAGEVTANNSSKVKIDSSKGEVKDVFVKKGDVVKVGQP
                     LFSYETSQRLTAQSSEFDVQTKANQLQVAKTNAALKWETYNRKVNEINTLKSRYNTAP
                     DESLLEQIRSAEDSVSQALSDAKTADSDVKTAQIELDKANATATMEKGKLEYDTVKSD
                     TAGTIVSLNTDLPNQSKSKKENETFMEIIDKSKMLVKGNISEFDRDKLKIGQKVEVID
                     RKDNSKKWTGKVTQVGNLKAEEKGQGQGQGGNDQQDNPNQAKFPYVIELDQSDKQPLI
                     GSHTYVNVLNNVPEAGKIVLKETFTMAENGKTYVWKVDKNKVKKQEVKTKPFSKGYIE
                     VTSGLTMQDKIAQPLPGMKDGMEVGSIVKP"
     gene            103928..104602
                     /locus_tag="VHD02_000094"
     CDS             103928..104602
                     /locus_tag="VHD02_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5496735.1"
                     /translation="MLNLKDIRKSYHLGTEEFAILKGIDLEVNEGDFLAIMGPSGSGK
                     STLMNIIGCLDKPGSGSYAIEGRDVSSLSDNELADLRNQKIGFVFQNFNLMPKLTACQ
                     NVELPLTYMNVPKKERRKRALEMLKLVGLEERSEFKPMELSGGQKQRVAIARALVTNP
                     SFILGDEPTGALDTKTSVQIMDLFKQFNDNGKTIIIITHEPEVAALCKKTVILRDGNI
                     EHSDIE"
     gene            104615..105832
                     /locus_tag="VHD02_000095"
     CDS             104615..105832
                     /locus_tag="VHD02_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ5496736.1"
                     /translation="MEDIIAALGSILSHKMRSILTMLGIIIGIGAIIAIFSIIEGNTE
                     NTKRQLIGGSNNTINIVFNKKSSIDPKFPDKSNAKKPDYLPFMAEEELSKIQQVKGVK
                     NALISYGIDDKVYHLGQKSSAKISAITKNVAEVRRMTFIKGSDFSDKDFIDQKQVIYL
                     EKSLYESLFPKDDGLGKFVEVMGNPFRVIGVFESKEQSGLTSGTEKIAYIPLHQWYNI
                     NGVVDATPEITIQTYRADDLKPVAKRVSDMLNQTIPKSDYMFGVMNLKEFERQLDNLN
                     KSNFVLLAGIASISLIVGGIGVMNIMLVSVTERTREIGIKKALGARRKLILKQFLIEA
                     VILTLLGGVIGVISGMVSGLIITRSLEYPYILSLFSVVLSLAFCCIIGIVFGLLPAIK
                     ASKLDPIEALRFE"
     gene            105926..106579
                     /locus_tag="VHD02_000096"
     CDS             105926..106579
                     /locus_tag="VHD02_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ5496737.1"
                     /translation="MFKILVVEDDDTISQVICEFLKANNYDPDCVFDGQAALDKWQTT
                     SYDLIILDIMLPSLSGLEVLKTIRKTSDVPIIMLTALDDEYTQLVSFNHLISDYVTKP
                     FSPLILIKRIENVLRVSTPDEKRQIGDLLVDETEHSVYWQGTLVKLTKKEYDIIDYLA
                     KRHQKIVTRDQLMDDIWGYSELDTRVLDNHIKNLRKKMTGIPLKTITGMGYLLGERE"
     gene            106576..107964
                     /locus_tag="VHD02_000097"
     CDS             106576..107964
                     /locus_tag="VHD02_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEQ5496738.1"
                     /translation="MRLIKKTFLVINGLIIVVVTSILLVLYFAMPIYYTKVKDKEVKR
                     EFDQTSKQIKGKTVTEIRDILTKKINKDNIWYSLVDSDNQLLYPSLQLLDGVSESKDS
                     QNVNIVTTFDNSYSNVKVMSQKVTLRDGKKMTLLGQSSLQPVTDASKVLLDLYPSLLI
                     FSVTVGSIVAYLYSRTSSRRILSMSQTAKKMVNLEPNLTCTIHGKDEIAMLASDINRL
                     YASLSTSIKSLQKEYEKASDSEREKSEFLRMTSHELKTPITSVIGMIDGMLYNVGDFA
                     DRDKYLRKCRDVLEGQAQLVQSILSLSKIETLASQNQELFSLKSSLEEEMEVFLVLSE
                     LKHLKVTINLEEQFVKANKVYLLKAIKNIIDNAFHYTKPGGQVMIQLKDNQLVIKNEA
                     ETLLTQQQMKQLFQPFYRPDYSRNRKDGGTGLGLFITHQILDQHHLAYRFVVLDQRWM
                     VFTIDFPSHHDD"
     gene            108066..109694
                     /locus_tag="VHD02_000098"
     CDS             108066..109694
                     /locus_tag="VHD02_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ5496739.1"
                     /translation="MKKRKLLAVTLLSTILLNSAVPLVVADTSLRNNPSSTDQPTTAD
                     TDTDDESETAKKDKKSKETASQHDTQKDHKPSHNHPTPPSNDTKQTDQASSEATDKPN
                     KDKNDTKQPDSSDQSTPSPKDQSSQKESQNKDGRPTPSPDQQKDQTPDKTPEKSADKT
                     PEKGPEKATEKTPEPNRDAPKPIQPPLAAAAPVFGLLRESDKDLSKLKPSSRSSAAYV
                     RHWTGDSAYTHNLLSRRYGITAEQLDGFLNSLGIHYDKERLNGKRLLEWEKLTGLDVR
                     AIVAIAMAESSLGTQGVAKEKGANMFGYGALDFNPNNAKKYSDEVAIRHMVEDTIIAN
                     KNQTFERQDLKAKKWSLGQLDTLIDGGVYFTDTSGSGQRRADIMTKLDQWIDDHGSTP
                     EIPEHLKITSGTQFSEVPVGYKRSQPQNVLTYKSETYSFGQCTWYAYNRVKELGYQVD
                     RYMGNGGDWQRKPGFVTTHKPKVGYVVSFAPGQAGADATYGHVAVVEQIKEDGSILIS
                     ESNVMGLGTISYRTFTAEQASLLTYVVGDKLPRP"
     gene            109772..110023
                     /locus_tag="VHD02_000099"
     CDS             109772..110023
                     /locus_tag="VHD02_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496740.1"
                     /translation="MIKKVTTPSQKTKKRVRNGYLLKVGTACLLLSILSYGIGLLGQP
                     SMENTFMGIASVAMLGSVCFFIIFALNRIFDALEDNLRD"
BASE COUNT        34308 a        20728 c        23329 g        32118 t
ORIGIN      
        1 agtaataagt tgaccaaaaa gccaggattg gttatgatga agataagatt aacagaaaga
       61 gaaattaggg aaggctatga aggatttatt aaaacagtat caggcaaaac cactaggaga
      121 ggagaagcgg tacgccgtat ttttacctct tattcttgtc aatgatgact ggcatgtctt
      181 atatgaagtc agaagtcagc atatttcaca accgggggaa gtttctttcc cagggggaca
      241 agtagaaaat caggagacac ttcaagaagc tgctatccga gaaacagttg aggaattgac
      301 tgttgatgcg tcccagatcc agctatgggg tgaaattgat tatttagtac agtcttctag
      361 aactattcat tgtttcgttg gtcaattagt cgttgatgac tgggaaagca tacagcctaa
      421 cgaagaagtt gacaaggtct ttacagtacc tttacgtcaa ctacttgtca ctgatcctgt
      481 ctattatcat ttagaagcca ctcctattga gacaacggat tttccttttg accgtattcg
      541 taatgggaaa tactaccaat ttagtcaaga gtaccgtagt attccgtttt acgaaaatct
      601 agaagaaacc atatggggaa tgactgccca gtttactaag tgtttaaccg atattctaaa
      661 tgatgaatca attgcaaaga aaaacccctt agatattgaa aaatctaagg ggagatagag
      721 cactagtttg ttggtgtcaa ctttttgtca acaacctgta aaggttattt gtatttttct
      781 tctaattgac tcatccaaag tccgagccaa ataccaagag caaataacac aacggccata
      841 agccaggtta agatacctgc atgactgtag gaaatggatt cgggactact actatttgga
      901 ataagaatta atagcgtgct ggataataca ataccaatga taaagtggta aacttttgaa
      961 tgatggtgct gtaaagcata atccatactt ttagaaaaag ccaagatggc taggactcct
     1021 ccgatagcaa taggtaagaa agtccctaat aaatccaaag atttgaatcc tattaacata
     1081 ggaccataga gtcctaagat aagtaagaga tttgacgggc ttagtccagg gaccagtact
     1141 cctaaagcaa ttaaggcacc tgctaaaata aaggttaaaa agttagctgg taaggtaccg
     1201 attaagtcat ttaagaagta aagacctagt cctgaaataa caaaggtaag gactagccat
     1261 agccagtcaa ctttgtctct ttgagactgc ttggttgact ccttaatcag actaggaata
     1321 gtgccgacaa tagcgccagc aaatccccat aagacgataa cttgatagtg cttaagcaaa
     1381 aactcaacgg ggaaagaaaa gagagcaata cctaaaatac caccaattcc tactggtaag
     1441 aaaaataaga cattttcaat aaagttatcc ctcatatgag ctaaaaagga aatcattcgc
     1501 tcatagattc ctaaaatggc tgcgagaaca ccaccggata caccaggtaa aataaaacct
     1561 agtgcaataa tcatgccttt aaaaactctt gaaataaatg aaaccattgt tgcctcctga
     1621 aaagaatgat tttattgtat cataaattta aagaattgct gacacaaagc tgcatatccc
     1681 tactaaaata attgttccta ataaataata cattcaattt tgaagatatt ttccttaaac
     1741 accatgtctt tgtgatcaag tcaagtttga ttgtcaaagt tgtaaataaa ttttacaaag
     1801 actgtaaacc ttacgataat tatcaaaaag ataaacgaaa aaaagcttag gtcaaagact
     1861 tttgatagta aacttattga agaaggatta tagtagctat taaaaaggag atttattatg
     1921 gactttaaaa accagttacc ttattattga aaaaaattac agatgtcaca agaagagctt
     1981 gctgaaatga tgtatgtttc aagaagacta tctcaaactg ggaaaacggt aaaagctatc
     2041 cagacctgca aaatcttttg atgttaagta agtccttaca ggttccttta gagcaactaa
     2101 tagcagaaga tatcccaaaa atgactttaa ttattaagga tgaggagatt aaacaatgtg
     2161 ataggaatac gcatattatg ttatttggga cactcctaat tgctatcagt atttatccta
     2221 taatgtcttg gttaggttgg tggggatatg gtccggttgc tatcttatgg ttgataatca
     2281 tgcgtagcgc cttaagaatt gagcgattta aaaagacata tgatgttcag acttaccgat
     2341 aacttgaagc tatagcaaag ggtgagagct tagatcagat tgataagatt aaagaaaagc
     2401 aaaatatcct tatcaaaaag catggattgt tattgcgtac acaggattga cagtgttaat
     2461 aaccctactc tctattttgt tttttcattt gtttttttaa acacgaaaaa agcctttgaa
     2521 acgttcattt cataggcttt tctcatctca attattttac ttctgtaaag gttaagtatt
     2581 ttctatttta gctgttataa accttattgt atcaatattt attgatagtt gcttaataaa
     2641 aggtagagtg aatggtagag tagttattgc gttagttcag caatcttttc aagataaata
     2701 aatacccaca gccccaagga attaacgctt ttgaccaata aaccacaatt ggttacgaac
     2761 tcgaacagga gaagagaaat aatagtctgt gtgttggatg acaatctctt ctttcagttg
     2821 tactcccgta tcctttagga ttttttgccc ctgttggtct ctaggaactt ttagaaatac
     2881 agtaatggca tcttttcgta tatctacgat acacttacgt acagctcggt taaacccgtt
     2941 atggattgga ttgatagtgg taacggtctg gtgttcaaag ttgcttacct tctgtactcg
     3001 ttcacgctgg aaaaggaagt gtcttaatag aaaggtgcga taaatagatt ggcagtagtt
     3061 cccaaggcta tctgtattca agcgttgtat catctttaat ccagataata aggctagaag
     3121 taaagaaata agagcgaatc cataacatac agaactaatg gtatcagtaa cgtttttaac
     3181 cgtatccaga ttgacaaatg tgacggtaga taattgaact gaaacaagat tgaacagcca
     3241 accgaacaca tagcagattg gagcaataag cagtaagtat aatatgagat tgaagcttct
     3301 catgaagtag gttttagtgg tcataggata cctcgcttag tatagtaggt tggacagagt
     3361 aagggtagaa cttgatagga gtgttcatta tcgacaactt gaatgatacc tgttccgtgt
     3421 cctgttggaa tgacaatccc ctctggatct aggtctggaa ataagaattg agtggttttc
     3481 tgattgatat ttccaatttg caataatacg tttagctgtt ccctcactga aatgggaata
     3541 gtgttatggt caaaacgctg gcttcccaag aatagatgga ttttagtagc atgtcccaac
     3601 aaggcaatct gtgatagtag ggagaaaaaa gcttctttga tattcttatt gactccctct
     3661 gatagagcga ggacttcatc aataacaata gttagatggg taaattgatg attgggatta
     3721 tcatacaaga tagcttgtcg tttctgaatg agagttgcac actggtttaa ttgttcattg
     3781 acttgtgaaa cgaaatcaga ttttgaatgg ttttctactg ggtggataac ggcaatttta
     3841 ttttctcttg cccattggct aggggtatcg aattttgggt caatgataat taggccggac
     3901 ataggtttca ggacactcaa gaagtaagtg agagcgtacg gtttcccaga acctgaattt
     3961 ccagcaatag cccagtgact ccccttatct aaattgagtt cgaagtgctt tatgacagga
     4021 attttcccct gttgtaagtt agatgaaaat ttatctaaat caggaatcgc caagcgttct
     4081 gaaatcccct ttctccaagg gaagaataat ccacgttgaa tgtgtaagtc atccgtctct
     4141 aaagggacaa aatctgtgca aattttgatt tatctatttt ttgacttccg tttgacttaa
     4201 tcgataaagt tgaccttgct tttttggaaa taactaatgg aacagaagta aggtaagact
     4261 ttggtatgtc ctctttatac attcgttcta atatatcatt taccataaat ccgatattaa
     4321 acaagggatt ttctctaagt ttttttatga ggttgggtat tatctctgtt gagacattct
     4381 cagaccacca cttattgatt attgagtcaa ttttatctag attaaatgtt gtcggttcac
     4441 catttttatc taattcgaac agtgtgttgc agacattaat ttcaaacgac ctaattagat
     4501 tatctctcaa catcaacaaa agaatatcag ccatttcttc aaaatcaata acagttaatt
     4561 catttattgg attattccta aaactcctag cggggttata actttttcca ccaacccata
     4621 cgtcaatgtc atctttcttg tgaaaagtat ccatttcagt ttcagtagaa atcataaatc
     4681 tttttctttg aatattgaaa tttgcaaatg tcttggcaat acttagacaa cttgattgga
     4741 ttgaaattaa atcatcactc atgaaagaat ataggttttc atctacagcc tctaaatctc
     4801 tatataaaat agaactgaaa aaataatgaa acagattctc aataaactca tctaactctc
     4861 caaaaacgat atacgtatag ctcgaagcgg ttagagcatt taaataaact aacatcgaac
     4921 tcgtaattgg tcgcttacca ctttcaactt gactaacttg acctgcattg cctagttcgc
     4981 tttgtgtaat attgtgtttt tttcttaaat ttttgatacg agtaggtatt tcttgtgaat
     5041 aattttcttc aaaaaatttc atatataatc cccctataat catggaaaag tagaaagtag
     5101 taaaaatagt tgttatccta gttgattaaa aaaatctctg atttcctcat cttttataaa
     5161 ataatatata attttcccct ctcttctaga gtccaagatg ttttgattgg ctagtttacg
     5221 aagatggtgg gaggcagatg ccatactgag atttaataaa caggctatat cgcagacaca
     5281 gagttcttcg acggcaagga gataaaagat gatatttatc tgtttattat cggtaaattt
     5341 tgataaaatg cgaagtgatt tttggacttt ttccttttca aggtagttcg ttgcggttgt
     5401 aacattttgt tgatttataa catccacttg acagatacta tcttttttca taattttttc
     5461 tcctagccta acacagccca tagcatgtca aagctgttgt tttcaatcag gatatacatc
     5521 cccaatccta aatagacaac ggcaataaac catctgctat atttttccaa agtttctcca
     5581 acagaaggga cttgtgctaa tttttgtgca gaaaaaacca agagataaat catgactaga
     5641 aaagtaagta aagtcactat caaattcgct aaatttaagg tggtaaaata tgggacaaag
     5701 acaccaatat tgtcagcgcc acaacttgca aaagtaatca tagcgactag aaaaatcagg
     5761 tttttattgt cttttcgcaa accatctttt gcaatagctt ctccatcaga atctcctaaa
     5821 agcaaaactt ttaggcctag gaaaattgga atcaaaccga gtaaacctaa aatctcttta
     5881 ctaggaatat aatttaagac aaatgcaaaa agcaaactta ggaatattag actaacagag
     5941 cctagaaatt gtcctaaata gatgttaatg atgtcttttc tgctttttct tttggcaaaa
     6001 aataacatta ggataataag taagtctacg gctgtcccag aatacaggat tattgaagta
     6061 acaacatttt gaatcataaa atacctcatt caaatatatt tttgaatgta ttctaacatt
     6121 aaactttgta gatgtcaact taaaatccac caaaatatag ataataagtt agtgtaccaa
     6181 atattaaaaa gccctgccat cgaaatgata gcagggctta acttcaatat ccagatgata
     6241 tatttatcta aaaaaggtag agtaaaaggt agagttttta ttgatttgta gtgcgatata
     6301 atgagtttca aaaaatcaaa aatcgcttaa aatcaatatt ttgacgtcta ttgacgtgta
     6361 ctgaaaccct tacttaacct cagtaaaaac aacgtgttta cgaagttttg gtgagtattt
     6421 tttcaattga agacggtctg gagtgttacg tttgtttttt gaagtaaggt acaagcgttc
     6481 accagattct ttatgttcaa gtgtaatatt tacgcgcatg gtatctccct tctattattt
     6541 agcttcgtta gctttagcga ttttacgtcc tttgtagtat cctttgagtg atacacggtg
     6601 tgaacgtgag taatctccag tagtttcgtc gaattgtaca gatggagctg tcaatttgta
     6661 gtgtgtacga cgtttgtttt tcttcgcttt tgaagtgtga cgtgcaggta ctgccatttt
     6721 cttattcttc ctttcgatgt gtaagttttg attttcatca acttcactat gataacatat
     6781 cttttttgta aagtaaagtt acttgacaaa actttttatt tttacaattt catggtgaaa
     6841 atactatcaa actaaagcaa atgatatatg cttgtttaaa atataagaaa aaatggtaga
     6901 atgtttaaag agaaaactaa tcgaagaggt aaacttatga aacttcaaaa accaaaaggg
     6961 acccaggata ttctgcctgg ggatgctgcg aaatggcagt atgtggaatc tgttgcgcgt
     7021 gatacgttta gccaatataa ttatggagaa attcgtactc ctatgtttga acactacgag
     7081 gtaatctcgc ggtctgtagg tgatacgact gatattgtga cgaaggaaat gtatgatttc
     7141 tatgataaag gagatcgtca cattaccttg cgaccagagg gtacagctcc agtggtgcgt
     7201 tcttatgttg aaaataaatt gtttgcgcca gaggtccaaa aacccgtcaa actttattat
     7261 atcggttcca tgtttcgcta cgaacgtcct caagctggtc gtttacgtga atttcaccag
     7321 attggggtag aatgtttcgg tgctgctaat ccagcaacag atgttgaaac cattgcgatg
     7381 gcctatcacc tccttgaaaa attgggtatt aaggatgtca cccttcattt aaatagtttg
     7441 gggagccctg agagtcgttc agcttatcgt caaaccttga ttgactactt aacaccaatg
     7501 cgtgatcaat tatctaaaga tagtcaacgt cgcttggatg aaaacccact gcgcgtgctt
     7561 gattctaaag aaaaagaaga taaactagcc gttgaaaagg cgccttctat tttggattac
     7621 ttagatgagg aaagtcaggc gcactttgag gcagtcaaag ctatgcttga agcacttgac
     7681 attccttatg tgattgacac caatatggtg cgtggccttg attactataa ccacactatt
     7741 tttgaattca tcacgtctgt ggagggttct gacttaacta tctgtgcggg tggacgttat
     7801 gatagtttgg taggttattt tggtggacca gaaacccctg gctttggttt tggtcttggt
     7861 ttagaacgtc tcttgatgat tattgaaaag cagggtcttg ccttaccaat tgagacagag
     7921 atggatgttt accttgctgt gctaggtgat ggtgctaata gcaaagcttt agaattggtt
     7981 caagccattc gccgtcaagg gtttacagct gaacgagatt accttggccg taagattaag
     8041 gctcagttta aatcagccga tacctttaaa gcaaaattgg taatgacttt aggagaaagt
     8101 gaagttgaag caggcaaagc tgtgattaag aataatcgta gtcgtcaaga agtggaagtc
     8161 agttttgaag atatgatgac aaactttgcg aacatctcag agcaattatt atcttaatga
     8221 ttttattgtc gtcataagct tgtgttgata tgattgttta gaagataatc actaaaacag
     8281 gtgtaacttc tctttttaga agggagttgc acctgtttgt tttctgtaag gggattgatt
     8341 agctttctta gtattctgtg aggacacctg atatgtcaaa atgctgacga ctcagtattc
     8401 ttgctttatc tttagaaaaa ctctttttaa atgggtgggg tttttaaggg ctttttgcta
     8461 taatagtagg gaatgaatta attgatttat atagtgaaag gaattggaaa cgagcaggtt
     8521 tcacaggatt ctgtttgttt tagggaattt atttatgaaa cgtagcatgt atgcagggcg
     8581 tgttcgcgaa gaacacattg gaacgacaat cacattgaaa ggatgggtca gtcgtcgtcg
     8641 tgacttgggt ggtctcatct ttattgattt acgtgaccgt gagggtgtga tgcaattagt
     8701 cattaaccct gaggaagttt ccagtgatgt gatggcaact gctgaacgcc tacgtagtga
     8761 atatgtgatt gaagtggaag gttttgttga agctcgacaa caagccaatg ataagttggc
     8821 gacaggaatg gttgagttaa aagtatctgc tttgaccatt ttaaacacag ctaaaacgac
     8881 gccttttgaa attaaagatg acgttgaagt aagtgatgac acgcgtttac gttaccgtta
     8941 cttagattta cgacgtccag aaatgttaga aaacttcaag ctacgtgcaa aagtgactca
     9001 ttctatccgt aattaccttg atgaccttga gtttattgat gtcgagacac ctatgttgac
     9061 aaaatcaact ccagaaggtg cgcgtgacta tctggtacca agtcgtgtta gccaagggca
     9121 tttctatgct ttgccacaaa gtccgcaaat taccaaacag ttattgatga atgctggctt
     9181 tgatcgctat tatcaaattg tgaaatgttt ccgcgatgaa gatctacgtg gtgaccgtca
     9241 acctgagttt acacaagttg acttggaaac gtcattcctt tctgaacaag agattcaaga
     9301 catcgttgaa ggcatgattg ctacagtgat gaaagagaca aaagaaatag acgtaaccct
     9361 accattccca cgtatgagct acgatgtagc aatgaatagc tacggctctg ataaaccaga
     9421 cacacgtttt gagatgcttt tacaagactt gacagtaact gtcaagggaa ttgacttcaa
     9481 ggttttctca gaagcaccag ctgtcaaggc gattgtggtg aagggaaatg cagatcgata
     9541 ctctcgtaaa gatattgaca aattaactga gtttgccaaa caatttggtg ctaaaggcct
     9601 tgcctgggtt aaggtgacag atggtcaatt ggcaggccca gtagccaagt tcttgacagc
     9661 tattgagaca gagttgtcaa gccaattaaa acttgctgag aatgatttag ttttatttgt
     9721 agcggatact cttgaggttg ctaataatac attaggtgcc cttcgtaatc gtattgccaa
     9781 ggaccttgac atgattgatc agtcgcagtt taatttcctt tgggtggttg attggccaat
     9841 gtttgagtgg tcagaagaag aaggacgtta tatgtcagct catcacccat tcactcttcc
     9901 aacgccagaa tctgctcatg agttagaggg tgatttggct aaggtacggg ctattgctta
     9961 cgacatcgtt ttaaatggtt acgagcttgg tggtggtagc ctgcgtatca atcaaaaaga
    10021 gatgcaagag cgcatgttca aggctcttgg ctttaccgct gatgaagcta acgcccaatt
    10081 tggtttctta ctagaagcga tggactacgg ctttcctcct catggtggct tggcaattgg
    10141 tcttgaccgt tttgtcatgt tgcttgctgg aaaagacaat attcgagaag ttattgcctt
    10201 ccctaaaaat aacaaagcct ctgatccaat gacgcaagca cctagtcttg tttctgaaaa
    10261 ccaattagaa gaactcagtc ttcaaataga aagtcatgat taagaaaaca acctataaga
    10321 aaaaagttaa atatgtcatt agtagagggg ccaagaaagt tggcctactc cacgctctaa
    10381 gaagtatttc aagagaaaaa tatgcagaga agatttcggc ttctctgctt tatggcattc
    10441 tctctagtat tgctgtgaat tttttcttcc agcctgggca tgtttattca agtggagcaa
    10501 ctggtctagc acaggttttt tcagctctta gtcatcgtct tttaggctat gattttccca
    10561 tcgcctttgc gttttatttg attaatattc ctttgcttat tttagcttgg tataaaattg
    10621 ggcatcaatt taccattttt acctttatca cagtcagcat gagttctttc tttattcaaa
    10681 tcatacctca agtgacgctg acgactgatc ctcttatcaa tgctattttt ggtggtttgg
    10741 ttatgggaat gggaattggt acaggtctca aatcacgtat ctctagtggg gggactgata
    10801 ttgtcagttt gacccttaga aaacgaacag gcaaggatgt gggcagtctc tcattgatgg
    10861 ttaatggtgc aattttagcc tttgcaggga ttttatttgg ctggcagtac gccctttatt
    10921 ctatggtctc tatctttgta tcaagtcgtg ttacggatgc cattttcacc aagcaaaaga
    10981 aaatgcaggc aactattgtt accagccatc cagagcgtgt gattcatatg atccataaac
    11041 gtctgcatcg cggggtgacc agtatcaacg acgcagaagg gacttacaag catgaacaaa
    11101 aagcagtttt gattaccatt ttgacatgtg aagaataccc agaattcaaa tggctgatgt
    11161 taaaaacaga cccacaagcc tttgtttcag tggctgagaa tgttagaatt atcggtcgtt
    11221 ttgtggaaga tgattaagaa aaacacagga ggtgtcatgt caattgacca acgtttacag
    11281 ttaaagaaat tttgctttat tattttagga gctgctatct acgcctttgc ttttgtttat
    11341 ttttacatgg ctaatcgcat tgcagccaat ggtttagctg gtctcaccct agttggtaag
    11401 gcactttttg agattgatcc ttctttgata ggctatttga ttaatttgcc tttggtcttg
    11461 ctgggagcta gatgttttgg gaaaagagcc atggtttaca cagtaacggg tattttatca
    11521 ctttactttt ttgtgtggct gtttcaacgc tttcctttgg tcgttgactt ggaccatgac
    11581 aatttagtcg tttccttgat ggctggaatt attggtggtc taggtggcgg tcttgtgttt
    11641 cgcaatggtg gaaccattgg tggagcagat attattgcta agttattaga agacaagtta
    11701 ggactacagc tgaatcaagc tcttttaggc attgatcttt ttgtcatgat tgtctctttg
    11761 acttatattt ccctgccgca aatgatgtat gctctgattg ctagctttat gtatggtcaa
    11821 attgttcgct tggtacaaca aggtggttat tcagcccgtg gcatttttat cgtttcagaa
    11881 caagcggaga aaattgcccg gttcatcatg gacgaattgg gaagaggagt tacttatcta
    11941 acaggtgaag gagcctactc tggtcgccgt aaaaaagtga tttatgtggc tttggggcga
    12001 agagatatta gggaattaaa agctttttta actcaggttg atcccaatgc ctttatcact
    12061 tattttgatg ttaatgaagt caatagccca gaatttttaa ctattaaaag caaataccat
    12121 aaaaagagaa aataaatgag cattagagtt gcctgcgaag aatagtgagg ataaaggatg
    12181 gcacatcacg ataaattgac taaactacta aaactgtttt tgattgccct aggtgtagcc
    12241 atttatacct tcggttttgt taattttaac atggctaatg ctttggctga aggtggtgtg
    12301 gcaggaataa ccttgatttt acacgcgcat tttgggatta atcctgctta ctcttctctc
    12361 ttatttaatc ttccactttt tattttaggg gcaaagattt ttgggaagcg ttctttggct
    12421 ctaaccatct atggaacagt tctgatgtcc gcttttatct ggatgtggca aaaagttcct
    12481 atcgaacttg gcttggaaaa tgacatgatg ttggtggctg tagtggcagg tctcttttca
    12541 gggattggga gtggcattgt ttttcgctat ggtgcaacca caggtggaac ggatattatt
    12601 ggtcgcattg cagaagaaaa atttggagct aagctaggtc aaactttgct tttggtggat
    12661 gctcttgtat tgacagcttc gttgacttat gtggatttaa aacacatgct ttatactttg
    12721 gtggcaagct ttgtttttag ccaaatgatt agtgtggttc aaaatggtgg ttacactatc
    12781 cgtggaatga ttattattac caagcattca gaggctgccg ctcaagctat cctaaccgaa
    12841 atcaatcgtg gagtgactta cttgaaaggt caaggagctt actctggtaa tgattacaat
    12901 atcatgtatg tgaccttgaa cccaactgaa gttcgggaag tcaaacgtat tttagctggt
    12961 ttggatccag atgcctttat ctccattatt gatgtggacg aagttattag ctctgatttt
    13021 aaaattcgcc gaagaaatta tgataaataa aaatacaggt caaacagcct gtatttttat
    13081 tttggtcccc cacttaagaa actaccaagc ataatagctg atatagcagt tcccttgtaa
    13141 tggctagctt ttgtttttac caagcgacta agatcaaata tgcttttagg agcatcaaat
    13201 tgatgtgtta agatgtattg attgataagt gagtcaattt ttgtagctag gagtgagctc
    13261 atcccctctt tttcaagttg actggcgagc tcgaagatcg cttgacgaaa ataatcatcg
    13321 gcttgtatta cttgttcgct ataaacttta ctaagagcat ctaatatttc tttttctgat
    13381 ggcatggcat tctcctgtaa catattgtgc tattattata atataaaaaa ctaagactgt
    13441 cataaatgtg acagccttag taatgttatt acttacattt catttggtgc gtcaacaccg
    13501 agtaagcgaa gcgcttcttt aagaacagta gcagttgcat agcagagagc taagcggtta
    13561 tcacgttctg agttgtcgtc aagaatgcga gtgtgagcat aatatttgtt aaagctttgg
    13621 gctaagttaa tagcaaattt agccataata gaaggctcaa agttatctga tgttcgctta
    13681 ataatgcgtg ggaaatcttg gataagtttg ataatttccc agctttcagc atcagctaaa
    13741 ctgtaagtgg tggttgcaga tggcgtaaag tcagctttac gtagaataga ttggatacga
    13801 gcatgagcat attgaacgta aggtccagtt tctccttcaa aggaaaccat ggcttcaagg
    13861 tcaaagtcat acccattcat acgatctgtt ttgagatcgt aaaacttgat ggcaccgaca
    13921 ccaacagcgt gtgcaacggc ttctttgtca gcaaggtttg gatttttcgc ttcaatttga
    13981 gaagctgcac ggttaatggc ttcagcaacc gtaggttcaa gaagaataac attaccttta
    14041 cgtgttgata atttagcacc gccttttgtg acaagtccaa aagcaacgtg tgtcatatca
    14101 tctgaccaat cgtaacccat ttcttttagc actgctttta attgtttgaa gtgagcagct
    14161 tgctcattac caacaacata aacggattta gcaaagtcat aagtgcgctt acggtaaagt
    14221 gcggcagcta ggtcacgggt aatgtaaaga gtagcgccat ctgatttttt gatcaaggca
    14281 gggtgttcaa tgccgtattt ttcaaggtta acaacttggg cacctttaga ttctacaaga
    14341 aggtttttag cttctaataa gtcaagaacc tcatccattt tgtcattata gaaggcctca
    14401 ccgttgtaac tatcgaaggt gacatgtaat tggtcataaa gtcggttgaa ctcaagtaaa
    14461 ctttcatcac ggaaccattg ccataattca gtagcttctt tgtcgccatc ttccaattta
    14521 cggaaccatt cacgagcttc ttcatcaacg gtgggatcag tttcagcttc agcattgata
    14581 cgaacgtaaa gttttaagag ttcatcaata ggatgtgcct gaacagcagc ttcatctccc
    14641 cattttttgt aggcaacaat gagcatacca aattgtttac cccagtcacc aaggtgattg
    14701 atttttacag gtttatagcc cattttggca aaaatgtgag cgagactatc accaataact
    14761 gttgagcgga ggtgaccgat tgaaaatggc ttagcgatgt ttgggcttga catgtcaata
    14821 gcaacatttc gcccttgacc ttcgtcttgc tgagcatagt ctgaacctgc tgtaatcact
    14881 tgttcaagga cctgtgagga aatcttagct ttatcaagga aaaagttgat gtaagggccg
    14941 acagctacga ctttttcaaa ttggctctca tcaatctgtt cagctaactc gctagcaatc
    15001 atttgagggg ctttgcgaag gacttttgct aaactaaagg ctggaaaggc aaggtctccc
    15061 atgtcagaat tttttggggt ttcaagcaag ttaaaaatag catcttgttc taattctgga
    15121 acaactttag ctatttcact tgcgattaga gttttagtat ccatacagcg tctcctaaaa
    15181 attgtttgtc accctattct aacatatttc tttaaaataa acgaggagag gattgataaa
    15241 ttatactgaa actttcaagg aaatgaattg cttgaggttg ttgtttttct gaaaatgtta
    15301 taaaaagaga aatgaggttt cttttcaaca gttttcaagt gaaattctag ttgaatttga
    15361 taaaaaattg ctgaaaatag ctgggcgaag atgaaataat acactttttt tgttataatt
    15421 tgagaggata attatacaat tggggtgata tatcatgaat aaaatggagc gtcaacagca
    15481 gattaagcga atcattcaag cagagcatat tggcacacaa gaagatatta aaaatcacct
    15541 tcaaaaagaa ggcattgtgg tcacccaagc aactctatct cgggacttaa gagaaattgg
    15601 ccttttgaaa ttgagagatg aacaaggcaa actctattat agtttgtctg aacccgttgc
    15661 aacaccattt agtcctgagg tgcgttttta tgttttgaaa gttgatcgag caggttttat
    15721 gctggttctc cataccaatt taggagaggc tgatgttcta gctaatttga ttgacaatga
    15781 tgccattgaa gatattctag gaaccattgc tggtgcggat accttgcttg tgatttgcag
    15841 agacgaagag attgctaaac gctttgaaaa agatttggct gcaggcctat gactagctat
    15901 caagagtctt tgacacaatt gattcttgct atccatgaat tctagtatcg ttgcttataa
    15961 agaagtgaag caatagctgt ctaagctcca tgattttaaa aagtaagctt atcagatgaa
    16021 acgctatcaa caagatgcct tgcttttcaa aaaaaaatag ataaagaaaa ggctgcgaca
    16081 gtatctgcaa gcagggcaaa agaactagaa gataggctca gtcatcagcc attattaatt
    16141 gatgattatc gagaaaagat gcaagatgca agtgatgtga ttcagtatat caccaaacgt
    16201 atagaagatc agttaaacaa ggagttaaca aatgacaaaa actaacattt ctcctggaat
    16261 gcaacagtat ctggacatca aaaaagatta tccagatgct tttttgcttt ttaggatggg
    16321 tgacttttat gaattatttt acgaggacgc tgtcaaagca gcacaactct tagaaattgg
    16381 tttgaccagt cgcaacaaga atgcggaaaa tccaattccc atggcaggcg tgccacatca
    16441 ttctgcccaa caatacattg atgtgttaat tgagttgggt tacaaggttg ctgtcgcaga
    16501 acaaatggaa gacccaaagc aagctgttgg ggtggtgaag cgtgaggtcg ttcaagtcat
    16561 aactcctgga acggttgtgg attcagctaa gccagatagc gccaataact ttttggtagc
    16621 tgttgacttt gatggttgcc gttatggatt ggcttatatg gatgtatcca caggtgaatt
    16681 ttgcgtgaca gatttggcgg actttacgag tgttcgtagc gaaatccaaa acctcaaggc
    16741 aaaagaagtc ttactaggtt ttgatttatc tgaagaagaa cagacgattt tggtcaagca
    16801 gatgaatttg ctgctttctt atgaagaaac ggtctatgaa gataaatctt taattgacgg
    16861 ccaattgaca acggtagaac tgacagcggc aggaaaactc ttgcaatacg ttcacaaaac
    16921 acaaatgcga gaactcagcc acttgcaagc cttggttcac tatgagatca aggattattt
    16981 gcagatgtcg tatgccacta agtcaagttt agatttggta gaaaatgcta ggactaataa
    17041 aaaacatgga agtctctatt ggctgttaga tgaaaccaag acagctatgg ggatgaggct
    17101 tttgcgctca tggattgatc gacctttggt ttctaaagaa gctattttag agcgtcaaga
    17161 aattattcaa gtttttctga atgcttttat tgagcgaacc gatttaagca atagtttaaa
    17221 aggtgtttac gacatcgaac gcttatctag tcgcgtgtct tttggcaagg caaatccgaa
    17281 agatttactt caattggggt ataccttagc ccaagtgcct tatatcaaag ctatcttaga
    17341 gtcttttgac agtccttgtg ttgacaaact tgtcaatgat attgacagtt tgcctgagtt
    17401 ggaatacttg attagaacag ccattgatcc agatgcacca gcaactatta gtgaaggcag
    17461 tattattcgt actggttttg atgagcgctt ggaccattat cgtaaagtga tgcgagaagg
    17521 aacaggttgg attgcggata ttgaggccaa agagcgtcaa gaaagtggca ttaataacct
    17581 aaaaattgat tacaataaaa aagatggtta ttattttcac gttacgaatt caaatcttag
    17641 cttagttccc gagcattttt tcagaaaggc aactttaaaa aattctgagc gttatggaac
    17701 agcagaattg gctaagattg aaggtcagat gttagaggct agggaagagt catctagttt
    17761 agaatacgat atttttatgt gtattcgagc tcaagttgaa acctatatta atcgtttaca
    17821 gaaactggct aaaactttgg caacggtgga tgttttgcaa agtttagcag tcgttgctga
    17881 aaccaatcat tatattcggc cgcagttcaa tgataatcat gtgattacaa ttcaagaagg
    17941 tcgtcacgcc gttgttgaaa aggttatggg agtgcaggaa tacattccca atagtatctc
    18001 ttttgaccaa cagaccagta ttcagctgat tacaggtcca aatatgagtg gtaagtcgac
    18061 ttatatgaga cagctggcct taacggttat catggcccag atgggttcat ttgtggctgc
    18121 tgatcatgtt gatttacctt tatttgatgc gatttttacg cgtattgggg ctgctgatga
    18181 tttgatttct gggcaatcaa cctttatggt ggagatgatg gaagcaaacc aagcaatcaa
    18241 acgcgcaagt gacaactctc ttattctatt tgatgaactg ggacgaggta cggcaactta
    18301 tgatggtatg gctttagccc aggcaattat tgaatatatc catgatagag ttggtgctaa
    18361 gaccatattt gcaacgcatt atcatgaatt gacagatttg tcaactaagt tgacaagtct
    18421 agtcaatgtt catgtagcaa cgcttgaaaa agatggcgat gttaccttcc ttcataagat
    18481 tgctgaggga ccggcggata aatcttacgg tattcatgtt gcaaaaatag caggactgcc
    18541 aaaatcccta ttaaagagag cagacgaagt tctgacccgt ttagaaacac agtcacgatc
    18601 tactgagata atgtcagtcc ctccacaagt tgagtcaagc agcgctgtta gacaggggca
    18661 attatccctt tttggtgatg atgagaaagc tcatgagatt aggcaagcac tggaagctat
    18721 tgatgtcatg aacatgaccc cgtttcaagc aatgacaact ctttacgaat tgaaaaagtt
    18781 gttatagtct ttcagctgaa aatgaaaaag atgctttcta tattgaaggc atctttttgt
    18841 tctgtcaaaa acggtccgag gccttcggct atttattaag tgtgttataa tagtccataa
    18901 gaatgcgagg aaattatgac aaacattatt gaattaccgg aagttctcgc caaccaaatt
    18961 gcagctggtg aagttgtaga aaggccagcg agtgttgtca aggaattggt tgagaatgct
    19021 attgatgcta aaagtagcca gattaccgtt gaaattgaag agtctggcct taagatgata
    19081 caggttacag acaacggtga gggaatgtct catgaagatt tacctttaag tctgcgtcgc
    19141 cacgctacta gtaaaattaa gagtcagagt gatttgttta gaattagaac acttggcttc
    19201 cgtggagagg ctttaccgtc tgttgcctct atcagtaaaa tcacgataaa aacagcaaca
    19261 aaagaagtca ctcacggttc tcttcttata gctactggtg gggaaattga gacacttgaa
    19321 gcgatctcaa ctcctactgg aaccaaaatt aaggttgaaa acctttttta caacacgcct
    19381 gctcgtctca aatatatgaa aagtttacag gcagaattag ctcacattgt agatgtggtc
    19441 aaccggttga gtttggcaca tccagaagtt gctttcacac tgattagtga tggtcgccaa
    19501 ttaactcaga catcaggaac tggcgattta cgccaagcaa ttgcagggat ttatggttta
    19561 aatactacca aaaaaatgct ggttatctct aatgctgatt tggattttga agtttccggc
    19621 tatgttagct tgccagagtt gacacgcgcc aaccgtaatt acatgacgat tttggttaat
    19681 gggcgttaca tcaagaattt cttgcttaat cgagcaattc ttgatggtta cggttctaag
    19741 ctcatggttg gacgtttccc aattgttgtg attgatattc agattgatcc ctatttggcc
    19801 gatgtcaatg ttcatcccac aaaacaagag gttcgtattt caaaagagcg tgaattaatg
    19861 gctttaatta gcacggctat ttcagagagt cttaaggaac aagatttgat tccagatgct
    19921 ctggaaaact tagccaaatc aagcacacga catttctcta aaccagagca aacacaactt
    19981 cctttacagt ctagggggct ttattatgat cctcaaaaga atgacttttt tgtcaaagag
    20041 tcggctgtct cggaaaaaat acctgaaact gatttttatt ccggcgctgt tgacaacagt
    20101 gtaaaggttg aaaaagcaga gctgttaccc cactcagaag aagttatagg accttcttcg
    20161 gttaaacacg caagtcgtcc ccagaatacc tttaccgaga ctgatcatcc taatcttgac
    20221 ttaaaaaaca gacaaaagtt atctcaaatg ttgaatcgct tggaaaatga agagcaatca
    20281 gtgtttcccg aattggatta ttttggtcag atgcacggaa cttatctctt tgctcaggga
    20341 aaagatggtt tatttataat tgaccaacac gctgcccagg agcgggttaa atacgaatat
    20401 tatcgtgata agattggcga cgttgacagt agtttacagc aattgttggt gccctatttg
    20461 tttgagtttt ctggttctga tttcattaac ttacaggaga aaatggcact cttaaatgaa
    20521 gtcggtatct tcttagaagt ttatgggcac aatactttca ttttgaggga gcatcctatc
    20581 tggatgaaag aagaagagat tgcatctggt gtctatgaaa tgtgtgacat gttgcttcta
    20641 accaatgagg tatctatcaa aacttaccga gcagagttag ctattatgat gagttgtaaa
    20701 cgatctatca aggcaaatca tagcttggat gattattcag caagaaatct gctactgcaa
    20761 ttggcccaat gtcaaaatcc ttataactgc cctcatggta gacccgtatt gattaatttt
    20821 agtaaggcag atatggaaaa aatgttccgt cgaattcaag aaaatcacac tagcctgcga
    20881 gagctaggaa aatattagag aggatgagat gcaagagttt ttaaaccttc ctaagcagat
    20941 tcagctgagg caactggtac gctttgtgac cattacctta ggcagtagta tctttccctt
    21001 tatggccatg tattatacga cttactttgg tacgttttgg acaggcctct taatgatgat
    21061 taccagtttg atgggatttg ttggaacttt atacggtggg catctgtcag atgctcttgg
    21121 tcgtaaaaaa gtcattatga ttgggtcagt aggaacaacg ctaggctggt ttctgactat
    21181 tttagctaat ttgcctaatg cagctattcc ttggttaacc tttgcgggta ttttattggt
    21241 agagattgct tctagttttt atggtcctgc ctatgaagct atgttgattg atttgactga
    21301 tgagagtaat cgtcgatttg tttacaccat caattattgg tttatcaata ttgccgtcat
    21361 gtttggtgca gggctatctg ggctttttta tgaccatcat tttttagcct tgttagtagc
    21421 cttattactc gttaatgtac tttgttttgg cgttgcttac tactattttg atgagactag
    21481 accagaaaca cacgcttttg atcatggtaa aggattactg gcgagttttc agaactaccg
    21541 tcaggtgttt caggatcgtg cctttgtctt gtttacctta ggtgccatct tttctggtag
    21601 tatctggatg cagatggata actatgtgcc agtccatttg aaactgtatt ttcagccaac
    21661 ggctgtgtta ggtttccaag taactagttc taaaatgtta tcattaatgg ttttaactaa
    21721 tacattgctg attgtccttt tcatgacagt agtaaataaa ttaacggaaa aatggaaact
    21781 attacctcag cttgtggttg gttctttact atttactcta gggatgctct tggcatttac
    21841 ctttacgcag ttctatgcta tttggctatc agttgttttg ttaacttttg gggaaatgat
    21901 aaatgttcct gctagtcaag tcctacgtgc tgatatgatg gatcattccc aaataggatc
    21961 ttatacaggt tttgtgtcaa tggcacaacc cctaggtgct attttggcta gtctactagt
    22021 atctgtcagc cattttacag gtcctttagg tgtgcaatgc ttatttgtag tcattgcttt
    22081 gctagggatt tattttacgg ttgtttctgc aaaaatgaaa aaggtgtagt atgtacgatt
    22141 atattaaagg tcaattgacc aaaattacgg caaaatacat tgtcgttgaa acaaatggac
    22201 taggctatat tatcaatgta gccaatcctt atagctttac agatagtgtc aaccaactgg
    22261 taactattta cctgcatcaa gtgattcgtg aggatgctca cttgttgttt gggtttcata
    22321 cggaagacga aaaagatgtt tttctgaaat taatttctgt atctggcatt ggccccacaa
    22381 cagctcttgc tattgtggca gttgatgata atgagggact tgtgaatgcc attgataaca
    22441 gtgacattaa gtacctaatg aaatttccta aaattggtaa aaaaacagcg cagcaaatgg
    22501 ttcttgactt agctggcaaa tttgtggagg ctccacaaga gactggtaat accaaagcgc
    22561 gcagcaataa agcaggcaat actcaactgg acgaagcgat tgaagccctc cttgccctcg
    22621 gttataaggc aacggaattg aagaaaattc gtgccttctt tgaaggaacc tctgagacgg
    22681 cagagcaata catcaaatca gcattgaaac tgttaatgaa agggtaggtg tttcacatga
    22741 aacgttgctc atgggttcct aaggataacc agctttactg tgattaccac gatttagaat
    22801 ggggtcaacc cctggatgat gatagagatt tttttgaatt gctgtgcctt gaaagttacc
    22861 agtcaggctt atcctggctg actgttttga aaaaacgcca agctttcaga actgtttttc
    22921 atcattacga cattgcatcg gttgcaacct ttacctcgga ggaaatagct gatgctttag
    22981 agaatccatc catcatccgc cacaagctaa agctagcagc aactgtcaac aacgctatcg
    23041 cagtacagaa aattcaaaaa gagtttggta gtttttcaac ttatctctgg aattttgttg
    23101 ggggaaagcc aattaacaac cttgtcaatc aagagaatct tgttccttct caaacagaat
    23161 tatctattcg cttagctaag gatttaaaga aaagaggatt taaattttta gggcctacaa
    23221 cggtttattc ttttatgcaa gcatcgggtc tggtaaatga ccatgaagag gcttgtgttt
    23281 tcaatagaat cttctaattc ttcgtgaatt ggaagatttt ttgataaaat aagatcagat
    23341 tacgaataga taagtaggtg gggaaatgaa agctgaactg attgcagtag gtaccgaaat
    23401 tttgactggt caaattgtga ataccaatgc tcaatttctg tcggaaaaaa tggcagagct
    23461 aggtattgat gtctattttc aaacggctgt tggggacaac gaggagcgtt tactttcagt
    23521 gattacaact gctagtcagc gtagtgactt ggtgatttta tgtggtggcc ttggtccaac
    23581 gaaagatgat ttaaccaaac aaactttagc aaagtacctt aggaaagact tggtttatga
    23641 tgagcaagct tgtcagaaac tagatgactt ttttgctaag cgcaagcctt catcacggac
    23701 accaaataat gagcgacagg cacaagtgat tgaagggtca atccctttgc caaataaaac
    23761 tggtcttgcg gttggtgggt tcatcacagt cgatggtatt agttatgttg tcttaccggg
    23821 tcctccaagt gaattgaagt cgatggtaaa tgaagaattg gtaccacttc tgtcaaaaca
    23881 atacagtaca ttgtattcaa aggtactacg cttttttggt gttggggaaa gtcagttggt
    23941 aacagttttg tcagatttta ttgagaatca aactgatcca actattgctc cgtatgctaa
    24001 gactggcgaa gtgactcttc gcttatcaac aaaaactgaa aaccaagctc tggcagataa
    24061 aaagttaggt cagctagaag cgcagctact atcccgaaaa actcttgaag gtcaaccctt
    24121 agctgatgtc ttttatggct atggggagga taattcctta gcgcgtgaga catttgagct
    24181 cttagtaaaa tatgataaga caattacagc agcagaaagt ctaaccgcgg gattatttca
    24241 gtcaactttg gcgagttttc caggagcttc tcaagtattc aatggaggct ttgtggctta
    24301 tagcatggaa gaaaaagcga aaatgctagg ccttccttta gaggagttga aatcgcatgg
    24361 cgttgttagt gcttatacag ccgaggggat ggcggagcaa gcaaggttat tgactggtgc
    24421 tgatattggg gtaagtttaa caggtgttgc cggaccagat atgttggagg aacagcctgc
    24481 aggtacagtt ttcattggcc ttgccactca aaataaggta gaatcaataa aggttttgat
    24541 tagcgggcga agtcgtttgg atgtgcgcta tatcgccact ttacatgcct ttaatatggt
    24601 ccgtaaaact ttattaaaac ttgagaattt gctataatgt tatttagtag ctatagtagg
    24661 atatggttta caattatgta gattaatcct gctgacgatg gtttgttaaa aaagaggaga
    24721 aagtattggc aaaaaaatta aaaaagaatg aagaaatcac taagaagttt ggtgacgaac
    24781 gtcgcaaagc ccttgatgat gctttgaaaa atattgaaaa agattttggt aagggcgcag
    24841 tgatgcgatt aggagaacgt gcagagcaaa aagttcaggt tatgagttca ggaagtctag
    24901 ctcttgatat tgcgcttgga gctggtggtt atcctaaagg acgtatcatc gaaatctatg
    24961 gtccagagtc ttccggtaaa acgactgtgg ctttacatgc tgtagcacaa gctcaaaaag
    25021 aaggtggaat cgcagccttt atcgatgccg agcatgcgct tgatccagct tatgctgctg
    25081 cgcttggggt taatattgat gaactcctct tgtctcaacc agattctgga gaacaaggac
    25141 ttgaaattgc aggtaaattg attgattctg gtgcggttga tctggttgtt gtcgattcag
    25201 tagcagcttt agtgccacgt gctgaaattg atggtgatat tggcgatagc catgtcggat
    25261 tgcaagcacg tatgatgagt caggccatgc gtaaattatc agcttctatt aataaaacaa
    25321 aaactatcgc tatctttatt aaccaattgc gtgaaaaagt tggtgtgatg tttggaaatc
    25381 ctgaaacaac accaggtggt cgagctttga aattctatgc ttctgttcgg ctggatgtgc
    25441 gtggaacaac tcaaattaaa ggaactggtg accaaaagga tagcagcatt ggtaaggaga
    25501 ccaaaatcaa ggttgttaaa aacaaggtcg ctccgccatt taaggtagca gaagttgaaa
    25561 tcatgtacgg ggaaggtatt tctcgtacag gggagcttgt gaaaattgct tctgatttgg
    25621 acattatcca aaaagcaggt gcttggttct cttataatgg tgagaagatt ggccaaggtt
    25681 ctgaaaatgc taagcgttat ttggccgaac atccagaatt gtttgatgaa atcgaccgta
    25741 aagtacgtgt taaatttggt ttgcttgaag aaagcgaaga agaatctgct atggcagtag
    25801 catcagaaga aaccgatgat cttgctttag atttagataa tggtattgaa attgaagatt
    25861 aaaaagagcc cttcagggct ttttaaaatg tatttttaaa ggttagtagg actactgttc
    25921 ataggtgtgt gggtgaatag aaaccatcat ttaaaaagat gtgagaaagc agaaattagg
    25981 ataactgata taaaattaaa ataactgata attcaaggga atacaggtga aagaccttat
    26041 tttaccaaga taatatcttt aaaattaaaa aaataggtcc aaagacctat atttatagat
    26101 aagaaactgt gctacaataa agctatcacg aaatagaaag tgagtcagca acatgattaa
    26161 aatttacacg atttcaagtt gtacgagctg caagaaagcc aaaacttggc taaatgccca
    26221 taagcttgct tataaagaac agaacttagg aaaagaaccg ctaactaaag aagaaatttt
    26281 agcaatttta tcaaaaaccg aaaatggagt ggagagcatt gtttcatcta aaaaccgtta
    26341 tgccaaagct ctcgattgcg atattgaaga attaagtgtt agtgaggtta ttgatttgat
    26401 tcaagataac ccgcgcatcc ttaaaagtcc cattttgatt gatgataaac gtctgcaagt
    26461 aggctacaaa gaagacgata ttcgtgcttt tttacctcgc tctattcgta atattgaaaa
    26521 tactgaagca cgattacgtg ctgcgctcta aaagctcttg tagagctttt tttaaatacc
    26581 ttcttcgctt gaaattcttg tttatttaat agaaataagc tataataaag catagcactt
    26641 aattttagaa agaaggtgta agtatgggat ttacagatga aacagtccgc tttaagttgg
    26701 atgatggcga caaacgacaa attagtgaaa cattaacagc agtctatcat tcacttgatg
    26761 aaaaaggtta taatccaatt aatcaaattg ttggttatgt cttgagtggg gacccagctt
    26821 atgttcctcg ttataatgat gctcgaaatc agattcgtaa atatgaacgt gatgaaattg
    26881 tagaagaact tgttcgctac tatttacaag gaaatggaat tgatgttaaa tgagaataat
    26941 gggacttgat gttggctcga aaacggttgg tgtagctatt agtgatcctc ttggctttac
    27001 agcccaagga cttgaaatta tcaagattga tgaggaaaaa gcagaatttg gctttacacg
    27061 tttagaagag cttgttaagc agtaccaagt tgaacagttt gttattggat tgccaaaaaa
    27121 tatgaataac accaatggtc cacgtgttga tgcaagtata acttatggaa accacataga
    27181 gcaccttttt ggacttccag tccattatca agatgaacga ttgacgactg ttgaggcaga
    27241 gcgtatgctt attgagcaag ctgacattag tcgtggtaag cgaaaaaaag tcattgataa
    27301 attagctgca caactcattt tacagaacta tttaaatcgg aatttttaag gagataaaaa
    27361 atgacacata atcatgaaaa tgaccaccaa cacgaagtta tcacacttgt ggatgaacaa
    27421 ggaaatgaaa ccttgtttga aattttatta acaattgatg gtcgtgaaga gtttggtaaa
    27481 aattatgttc tcttggttcc agcgggatct gaagaagatg agtctggtga aattgaaatc
    27541 caagcctatt catttactga aaatgaagat ggtactgaag gggatttaca accgattcct
    27601 gaggattcag atgctgaatg ggatatgatt gaagaggtat ttaatagttt tctagatgag
    27661 aactaaattg ttagtgacac tatgttaaaa agtagccgtg agggctattt ttttaatgtt
    27721 attttcgaat aaataagcag gaggtgataa tgataaaaga attaaatgat aaatttatta
    27781 ataattataa ggctaaacct ttgcatagat ttagagaatt attacagtat gtgaaaatat
    27841 atcagaaatt gactaaataa aagattctgt tatacttgtt ttataatatt ctttgatgat
    27901 aatatcattt ttcaacttac tttgtttatg cactgtcact ttgctatgtg caatacaaag
    27961 tcatgttatt taactcatac taaaaaataa tggataatag ctatgttaga ctagtgacat
    28021 gaagaaatat tgcttttatt gaatcgttag agaatatgat atttcaagga gaaaaatagt
    28081 gatcgagtta aacgatgaat tcatccgaaa agagacgata gaactggcca acgatggccc
    28141 acgagtacac accactcaat atgaaactaa agtacctcgg cttcataaat gctatctgct
    28201 tttctttagc attattatca gttctttaac aattgcagta ccttttttaa cagatgcagc
    28261 caatggtctt cagtcccaaa acttatatat aggaatgatg ttaacgaagg ggcaacttcc
    28321 ctatagtgat acttttacaa caggagggct cttttacttt gttatcattg cattaagtta
    28381 ttatttggga tcgacacttt ggctagtctt tgttcaggtg ttttgttttt acttatctgg
    28441 tttatatctt tataaactca ttaattatat gacaggcttt caaaaagtgg ctttaacttt
    28501 ttcaattagc tactatttgt tgtctgttag tcttggtttt gggggattgt atcctactca
    28561 actggctatg ccatttatat taatatcggc ttggttttta actaagtatt ttgcctgttt
    28621 agtgaaagat gaggcattta ttctttttgg ctttgtaggt gctcttgcaa tgctaattga
    28681 cccgagtacc cttatctttt ggtcttttgc ttgtgtgaca gttttttctt ataatataag
    28741 ccaaaagcat cttgcaagag gtttttatca actgctagct tcgatttttg gaatgatttt
    28801 agttttttac acagcaggat atttcatttt gaacttacaa gtgctaaatc cttatttatc
    28861 acaaacgatg atttatcctt ttactttttt taaatcagga aacttatcgt tgctttttgg
    28921 actggctatt cagttgttct tcgctttggg gcttggtctt ttgacgggga tggagaatgt
    28981 cattaggcga tttaaaaaca attctgatag ggtcgtcaag tggctatttg tcatggtcat
    29041 tctagaatct atacttgtgg ctatattttc acaagactat cgcccctatc atcttttacc
    29101 tcttttacct tttggattaa ttttgactgc tattcctgtt ggctatcagt atggtatagg
    29161 attaggtcag agtagtcatc gcagacgtca tggtaaaaat ggtgttggtc gagtaatgat
    29221 gatttatctt aagagacact tttatttgcc aattttaatt gtagggacaa tactaatctg
    29281 ttctacttat tgtttcatta gtagtattcc tcttaatcag gagcgtgatc atattgctag
    29341 ttatttagaa cagaaactaa ataaaactca atctatttat gtttgggatg atacttctaa
    29401 aatttatttg gacagtaaag ctaaatctgt ttctcaattt agttctcctg acatcaatac
    29461 gcaaaaagtg agtcatcgaa aaatattaga agatgaacta ttagaaaata aggctgctta
    29521 tatcgttgtt aaccgctata aaaacctgcc taaaatcatt caaaaagtat tatctactaa
    29581 ttacaaagta gataaacaga taacgacaaa aagttttatt gtttatcaga aaaagtaatt
    29641 taatatcaat atattgtgtt cgagcaaaaa acttgacaca atatattgat ttttttagtt
    29701 gtcgtgatat aatagttccg taaggagatt tgatatggta agtttagaag aagacaaggt
    29761 gactgttcaa cctgatatta aagtgattaa acgagatggt cgccttgtta attttgatag
    29821 tacaaaaatc tatagtgctt tattaaaagc aagcatgaaa gtaactcgga tgtcgccact
    29881 tgttgaggct aaattagagg ctatttctga tcgcattata gcagaaatta ttgagcgttt
    29941 tccaactaat atcaaaattt atgaaatcca aaatattgta gagcataagc ttcttgcagc
    30001 taatgaatat actattgcaa aagaatacat taattatcgt actcagcgtg actttgcacg
    30061 ttcacaagca acagatatca atttttctat tgataaatta attaataaag atcaaacagt
    30121 tgttaatgaa aatgctaaca aagatagcga tgtttttaat actcaacgag atttaactgc
    30181 tggaatcgta gggaaatcaa ttggtttaaa aatgttacct tcgcatgttg ctaatgctca
    30241 tcaaaaagga gatatccatt accatgattt ggattacagt ccttatacac cgatgacgaa
    30301 ctgctgttta attgacttta agggcatgtt agccaatggc tttaaaattg gtaatgctga
    30361 agtggaaagt cccaagtcta ttcaaactgc aacagctcag atctcacaga ttattgcgaa
    30421 tgtagcatca agtcagtacg gcggatgcac agctgatcgc attgacgagt ttttagcccc
    30481 atatgcggag cttaacttta aaaaacatat ggctgatgct aagaaatgga tcgttgagac
    30541 taagagagaa agctatgctt ttgaaaagac tcaaaaagat atttatgatg cgatgcagtc
    30601 tttggaatat gaaattaata cgctctttac gtctaatggt caaacaccat ttacttcttt
    30661 aggatttggt ttggggacgt cttggtttga acgtgagatt caaaaagcta ttttgaccat
    30721 tcggattaat ggtcttggta gtgaacatcg cacggctatt ttccctaaat taatttttac
    30781 ggttaaacgt ggcttgaatt tagaaccaga ttcaccaaac tatgatatta agactttggc
    30841 tttagaatgt gcgactaagc ggatgtaccc ggatatgtta tcttatgata aaattattga
    30901 tttgacagga tctttcaaat ctccaatggg atgccgctct ttccttcaag gctggaaaga
    30961 tgaaaatggg caagatgtga cctcaggccg tatgaatctt ggggttgtca ccctcaattt
    31021 acctcgcatt gccatggaat caaatggcga tatggataag ttttgggagc tgtttaatga
    31081 gaggatgcta attagtaagg atgctttaat ttatcgtgtc gaacgtgtca cagaagcaaa
    31141 accagcaaat gcccctattc tttatcaata tggtgctttt ggaaagcgtt tggagaagac
    31201 agggaatgta aatgatctct ttaagaatcg tcgtgcaaca gtctctcttg gctatattgg
    31261 cctttatgaa gtggcgtctg ttttttatgg tggtcaatgg gaaggtaatc cagatgctaa
    31321 agcttttacc ttgtcaattg tcaaggcaat gaaacaggcc tgtgagaatt ggtcagatga
    31381 atatggttat catttctctg tttattcgac tccatcagaa agtttgacag atcgcttttg
    31441 tcgtttagat acggaaaaat ttggcattgt gacagatatt acggataaag aatactatac
    31501 aaattctttt cactatgatg tgcgtaagag tccgacacct tttgaaaaat tagattttga
    31561 aaaagattat ccagaagcag gtgcttcagg tggttttatc cactactgtg agtatcctgt
    31621 tttgcaacaa aatccaaagg ccttggaagc ggtttgggac tatgcttatg atcgtgtggg
    31681 gtatttggga accaatacgc ctattgataa atgctataat tgccaatttg aaggtgattt
    31741 taccccaaca gaacgtggtt ttacttgccc aaactgtggc aataatgacc ctaaaacagt
    31801 tgatgtggtc aaacgtacat gtggttactt ggggaatcct caggcccgcc caatggttaa
    31861 cggtcgccat aaggaaatct ctgcgcgtgt aaaacatatg aatggttcta ctataaaata
    31921 cccaggcctg taaactcaag ctgggggaga gcccttttga tggaaatatg atatgcttaa
    31981 aaggttgctt catgcaagca atcttttatt gtctaatggt ttcttagcaa ttgtgagata
    32041 agaataacta atccaaaacg actaattagc tgttagcaat cggactataa gaagaaagag
    32101 ataaggaaga aataatggga aaatatcaat tagactataa aggaatgcag caggtagaac
    32161 gctttcatga gaagcattct aaaaaaaaga cggataaaaa atcccgcgtt caagaactta
    32221 aggctcggtt tttagagaag tcaaaaaaac aggataacta aagtatccaa taaagaaaga
    32281 aggttgagta tgcttaatat tgggattgtg ggactaggtg ccatttcaca aaaagcctat
    32341 ttaccatata tgagacagct gagtgacatt acttggcatc tatcaacgcg gaatgcagca
    32401 gtacgtcagc aggtcggtca gttatttggt cacgctattc tttacagtga tgtcaaggag
    32461 ttgtcaaaaa caaacttaga tggggtcttt attcatgctg cgacaaaagc tcatgttgat
    32521 ctagcaagct tatttctaag acaaggtatt ccagtattta tggataagcc attggcagat
    32581 aactacgact cagtaaagta tctatacgag ttggctgaag ctcatcacac ttttttaatg
    32641 gctggtttta ataggcgctt tgcaccatgt gtcaaggaat tgtcaagttt gtcaactaag
    32701 cgtaaagtgg cggctgaaaa aaatgacttg aatcggccag gagatatgac ttttaagctc
    32761 tttgatttct ttatccatcc tttagataca gccttattct tgacagaagg gaccttacta
    32821 aaagggcatt ttcaatatca tctggaagca ggcttactga gccaagttat ggtaaccttg
    32881 atgaccgaaa gcatgacgac tacagcttct atgaatttac aatcagggag tcgccgtgag
    32941 gtaatggaag ttcaacgggc tgaagaaact tatcatttag agaacttaga cgagttatct
    33001 atttataaag gtactgaaaa aagggtgctt ggctttgctt cttgggacac taccttgcac
    33061 aaaagaggtt ttgaaacaat gatcgatgcc tttttagaag ctattagcac aggcgtcaat
    33121 cctgtcagcc ctgagtctag tcttttaagc cactggattt gtcaacagat tgctgactct
    33181 cagctttctt atggagagtt aacggtagag ttgcctaagg attaggaatt gatatggaaa
    33241 taagacgacc gactttaaaa gataaagatg ctgtattgtc aatgattaac gaatttttag
    33301 agcagaaaag tgctacagat gggttatggc attttaacgt caatgacttc aactatgaaa
    33361 catggttgga agattccttg cgacaggaaa tgggtttggc cagtcaagga gtgcctgcta
    33421 ttcaatatgt ggcgtttgat gagaaaaatc aagctttggg atttttaaac cttaggttac
    33481 gtttaaatgc ctcgttactt gaaaaaggtg ggcatattgg ttactctgtc cgtcccagcc
    33541 agcgtgggaa aggttatgcc aaggagatgc ttaaacaagc tgtcagctgt gcgatttcca
    33601 agaatatcac gacaattttg gtaacttgtg atgagactaa tgttgcaagc cgagcagtta
    33661 ttgtggctaa cggcggtgtc ttggaagata gtcgaggtgg aacggaacgt tattggattg
    33721 aagaacaaag aggcgataag tgatggcaga aaagtgttgg aataatccta agccaaagga
    33781 atggcaggca gaggagttaa gtcaaggccg gattattgac tataaggctt ttaactttgt
    33841 tgatggtgaa ggggttcgta attcccttta tgtgtctggt tgtttattcc attgcaaggg
    33901 gtgttataat gctgcgactt ggtcttttaa ggcgggaatg ccttatacgc aagaacttga
    33961 agagcagata atgacggatt tggcacagcc ttatgttcag ggactgacac ttttaggtgg
    34021 agaacccttt ttgaatacgg gtatcttaat tcctctgatt aagcgtattc ggtgtgaatt
    34081 gccagaaaaa gatatttggt cttggacggg ttatacttgg gaagaaatga tgcttgaaac
    34141 accagacaaa ctggaaatgc tgtccttaat tgatattctg gtagatggtc gttttgatat
    34201 cacaaaaaag aacctcatgt tgcaatttag aggctcttct aaccagcgaa ttatcgatgt
    34261 tcaaaagtct ttggctgcta aagaagtgat tatctgggat aagttaaacg atggggacca
    34321 aacctttgaa caaattagtc gggaggattt gttttaagaa gctgacttat ccacatgatt
    34381 tttttgaaaa ggtcatgtga aaacaggtcc tttaagggtg aattggggag aaagtaattg
    34441 ttattccctg gttttcccca cattatcctc atagttatcc acaggttgtg tataagttgt
    34501 agacttgtga ataaagacta aaaaagaggc ctaggcttct tttttgatat agacaaattc
    34561 atgatctgtt gataggtctg gatggtagta aaaaccagca aagacaaggc ttttaaggct
    34621 gtcaacagtt tggcaattat tttccataca ggcagtgaga aatgcacctc gtgccttttt
    34681 tgaaatggtg gaatgggttt tgaatcggcc ctctttttct gccataaact tgggacttat
    34741 ccacaattgt ttacagtctt tggaaaagac atcgtcaaat tcactggata aaagggaaat
    34801 cacttgggga tgctctttgg caaactgatt ataacagggc cgccagtaag acttgaggct
    34861 ttgtccttca atttttattc tggtgtgaaa atcatggcgg tgctctgcga tagggtgatt
    34921 agcgggaatg ataccgtaaa aggatgaggt gatgtaaacc tgctgggtga ggtaagcctg
    34981 ttcttgggta gtgagcttat cgcgcttgat atggcggtac ataaggccat tgaacagttg
    35041 gtaggcaggg taagcgaggc tttgttgaga ggccatgtct tgccagcgtt gctgttcttt
    35101 tttagctgcc tcttctttga tacgatatga tttggccaag tcctctgtgg tcatagctgc
    35161 cattgccttt aaaatggcct ggcttggttg gggcagtagg tgtggataag attctttagg
    35221 aatcaccatt tcttttgcgg ttggaattaa aaaggttagc atagtgttat tgtagtaaag
    35281 ttacttgctt tctgtcaaga aaactagtct tgtccaagga tgacgtccac acggatacct
    35341 tcctcgtctt ctgtcatgaa agaagaagaa tcttcttgta aaatagctag gccatatcat
    35401 ctggcttttt tgagagtagc tgaaaaaagc aggggcgtct caatgtgaat ggttaagaag
    35461 gctagacctg gagtgccttc ttggtgtttt ttgaggtaag gggctgacca gtgattgaat
    35521 gcaaggtgat gatggtagtt ccctgaagca atccagctag cacttggaat ggtcatttta
    35581 tcgcctaaat caaaaacctt ttgatagaga agtgaagaat ccagagcatt tttgacactc
    35641 agatggacat gactaatgcg tgtgtcttga gctagtaaaa agtgcttggg gatatctgtc
    35701 aactgctcca atatgctttt ggtatccgtg ggctcagtca caccaataat ttgaccattg
    35761 tcgcggatat cccactgctc aacagctttg tcatgataga tttcaatccc attaccctca
    35821 ggatcgctga ggtagatagc ttcgctatag ccgtgatcag ctgctccttc taaagagata
    35881 cctctggtta agaaatgaag tcccaagcta tgacgatcag gaactaaaaa ggctgtgtga
    35941 taaagaccat aagccttatt tgctggtaaa ggtgtttgtc gtaactctaa gataacggtt
    36001 ttaccgtctg tagtcaattg acgagatgtc gtgtcttgtg agagaacctg taaaccgata
    36061 atacttgtgt aaaaggttgt catctttgct agatcggtaa cattgaggga aacctttcct
    36121 agagagatgg ttgagttgta agggtatgtc atcctattgt ctccaaaagt gattaagcct
    36181 atgataactt attagtttca aaaagaaaag taggcacaaa taagtggtat aggcactaaa
    36241 aagtaactca tttgtcagcc ctatcttgtt tctgttctct ctaatagtct ataatgggtt
    36301 ataagttaca aaaagatact aagaggagct gctgcctatg ctgaaaaaaa gaccggcttg
    36361 tcccgtagaa acaaccttat cggtaatcgg gaataaatgg aaattattaa tattaaggga
    36421 cttgctaaag gggaccttgc gttttgggca gttgaagtcc tcaataggtt cggtcagtca
    36481 aaaggtactt actgcccaat taagggctat ggaagcagac ggattggttc atcgggaatt
    36541 gtatgcagaa gtgccacctc gggtggagta ttccttaaca gaaactgggt tgagcttagc
    36601 tcctgttatt gaagcgatgt cggattggga ccaaacttac caagaaaaac atgccgattt
    36661 atttgaagaa ggctagagga aagagttgtc cgagcatact gagacttaga gaagctgaga
    36721 gtttttttaa aatgatatta tcaaattctt tacagtttga taaaattact ttttttctac
    36781 aaaaagactt gcgctcatct cacgaatatg ataatataat atcaagtctg tttgagaaaa
    36841 cagaagctca ggtcaagtaa gtgcacactt acaataacac agttgataat ggggaaactc
    36901 agagtcaaga aaaagaaaag caagccttgc attttgcaag gcttttttct gtctttattt
    36961 tggtctgcgc cgagaaaaat ctaaaaattt aaatttttcc agtttatggc gactctcggt
    37021 gaattggaat tggttattat tggaaaggta aactttggat ttgacggaaa caacatgttg
    37081 gtctgatcca aggtcaagta aaattttatc acgatcggtt atttggtcaa tggtaacctc
    37141 tttgagggca aagtcaatgg cgagatggag ttccttttcc aaatattgat aaatagaatg
    37201 ctctgcgatt tcccttgtca ttattggcac caaagtttta ctaaggtaat caatgtctaa
    37261 gacggaagcc acaccctcaa caacgcgttg gcgcgtaaca cgccagacca agctgttttt
    37321 gctaaaaccg gtcaattttg aaagtgtctc atccacaatc agtttatcaa tggcaatgac
    37381 gttggtctta gaatctagat ttgagtaaga gacgagttcc tgataactag ttagttctga
    37441 gatagggaac atgatctgat gatgtttaat cacttgggtg ccacgtcctt gttttttgag
    37501 gataagacct gcctttgtga gaagtgagag agccttgcgg acggtatctc ggctagcttg
    37561 gtattgttga ctcaattcta tttcagtagg taaaaaatcc ccttctttgt aaaagtcttt
    37621 gtttattttt gtttcgaggt ctttataaat acgttcatac tttgtcatag ggtaaattac
    37681 ctttccactt gtctattttt ttacagacaa caccttttat tttaccaaaa taatggcttt
    37741 tttggcacaa acaacttgca gacaaatttg caatcgatta caataaaggt gtcaatcata
    37801 ttttatctaa gaatttgaat atttcatttt gacgttgctt gttaaaagca actagaacaa
    37861 aggactaggg gatgtcacat gggaaaattt gaacaggatg ctaaaagtct tctaactgct
    37921 attggcggta aagaaaacat caaggctgtc acacactgtg caacgcgtat gcgttttgtt
    37981 ttgaatgata ataataaggc aaatgtcaaa gagattgaaa aaatctctgt agttaaaggg
    38041 acatttacca atgctgggca gtttcaggta atcattggta atgatgttcc agttttttat
    38101 aatgacttta cagctgtttc tggtattgaa ggggtgtcta aagaagctgc caaatcagca
    38161 gctaaaagta atcaaaatgc cttacaacgg gtgatgacca tgttggctga gattttcaca
    38221 cctattattc cggcgattat tgttgggggg cttattttag gtttccgtaa tattttggag
    38281 agtgtgcctt ttgaatttct tgggcagcag gtcgaaaaag ggaaattagt ttttgatgca
    38341 gctggggatc ctgtttggaa tacgattgtg agggtatctc ctttctggtc aggggttaac
    38401 catttcttgt ggttaccagg ggaagctatt ttccacttct taccagttgg gattacttgg
    38461 tctgtgacgc gtaagatggg aaccactcaa attttaggga ttgtccttgg tatctgtttg
    38521 gtgtcaccac aattattgaa tgcctatgcg gtagcaggaa cgcctgctgc tgagattgcc
    38581 aaaaactggg tttgggattt tggtttcttt accattaatc gtattgggta tcaggcacag
    38641 gttattccag cccttttagc tggtctgtcc cttgcttatc ttgaaatttt ctggcgtaaa
    38701 cggattccag aagtggtttc aatgattttt gtgccattcc tttctttgat tccagctttg
    38761 attttagcgc atacggtatt ggggccaatc ggttggatta ttggtaaagg gatttccttt
    38821 gttgtgttag ctggattgac tggtcctgtt aaatggctat tcggtgctat ctttggtgcc
    38881 ttgtatgctc cgctagttat tactggttta catcacatga caaatgccat tgatacccaa
    38941 ttaattgctg atactgcaac tcgtacaact ggtttgtggc caatgattgc tctttcaaat
    39001 atcgctcaag ggtcagctgt ttttgcttac tatttaatga atcgtcatga agaacgtgag
    39061 gctgaaatat cgcttcctgc agcaatttct gcttaccttg gggtaactga gcctgcctta
    39121 tttggggtta atgttaaata cgtttatccc tttgtagccg gaatgattgg ctcaggtatt
    39181 gcgggtctct tatcaacaac ctttgatgtt caggcaaatt ctattggtgt tggtggctta
    39241 ccaggtttca tggctatcaa tgtgaagtat atgattccat tcttcatctg tatggcagta
    39301 gccattgtgg taccgatgtt tttaaccttc tttttccgta aatcacatat catgaccaag
    39361 acagaagatg aggctaaact agctgagaca cccgtttcgg aggctcctgc ggcaactgct
    39421 ccacataaga ctatgcaagg aacagttatc actttaacca gccctttaac gggtgaagtt
    39481 aaagcgttgt ctgaagctgt tgatcctgtc tttgcacagg gagttatggg ccaaggtgtt
    39541 cttcttcaac cgacagaagg ggtgttagta gcgccttgtg atgctgaagt atcggtcttg
    39601 ttcccaacca aacacgctat tggtttggtg acggctgaag gtttggaatt attgatgcat
    39661 attggtatgg atacggttaa cttagatggt caaggatttg aagctttggt gaagcaaggt
    39721 gatcaggtta aggctggaca aacattgatt caatttgata tagcagctat ttctgaagct
    39781 ggatacgcca ctgaaatgcc tcttgtggtg actaatcaag atacttttac ggtaactgtt
    39841 gaaggtagtt taccgcatca gattaaggct aatgataagc tagtagtagc ggtgaaaaag
    39901 taggtttact aagagaaaac gttcaggaac tgagatcagt tcctgaaact attttaggga
    39961 ggaaaactca tgacaattga taaaaagaaa gtcgtctatc aaatttaccc aaaatcctat
    40021 aaggacacta ctggaaatgg tgtgggagac ttgcgaggga tcattgataa attgccttac
    40081 ttacaagaac taggaataga tatgatttgg ttgaaccctt tctaccctag tccacaacga
    40141 gacaatggct atgatgtttc agattatacg gcggtcaacc ctgattttgg gacgatggct
    40201 gattttgaag atttggtaaa agctgctaag gagcatcaga ttgagttaat gctggatatg
    40261 gttttgaatc actgttccac agaccacgag tggttccaaa aagctttagc aggagaccct
    40321 tattatcagg atttctttat cttgagagat cagccgactg attgggtttc caaatttggt
    40381 gggaatgctt gggcgccttt tggagataca ggcaaatact acttacactt gtttgatgtg
    40441 acacaggctg acttgaattg gcggaaccca catgttcgtg aggaattggc taaagtggtt
    40501 aatttttggc gagataaagg agtgaagggg ttccgttttg atgtgattaa cctgattggg
    40561 aaagatgaag agctggtgga ttgtccggtc aatgatggta agccagctta tacggatcgt
    40621 cctattactc acacttatct tcatgatctc aatcaagcca gttttggtca ggatgattcg
    40681 tttatgacag taggggaaat gtctgccacg actattgaca actgtctttt atacacggct
    40741 cccgaacggg aagagttgtc catggctttt aatttccacc atctaaaagt tgattatgag
    40801 aatggtcaga aatggactat tatggctttt gattttgcag cgctgcgaga cttattccat
    40861 gcctggggtg aaggcatgag tcaaggcaat gggtggaatg ccttgttcta caataaccat
    40921 gatcaaccac gtgccctgaa tcgttttgtt gatgtaacac atttccgaaa cgaaggtgca
    40981 acgatgttag cggcttccat ccatttgtca cgaggaacgc cttacattta tatgggtgag
    41041 gagattggca tgcttgatcc agattttgat agtatggata attatgtgga tgtggaaagt
    41101 ctcaatgctt attcaagctt actagtctca ggtaaaagtg cagaagaagc ctttgccatt
    41161 atcaaggcta agtcaaggga caatgccaga acaccaatgc aatgggatgc tagtgaacat
    41221 gctggcttta cgactggtaa gccttggtta gaggttggca aatcttatcg agacatcaat
    41281 gtcgaaacag aaaaagaggg acgtattttt cctttctacc aacgcttgat tgctttgcgg
    41341 aaggaactcc ctattattgc tgaaggggac tatcgggctg cttttaaaga tagtcaggct
    41401 gtctatgcct ttgaacgcca tttaggtgac cagtgtttgc ttgttctcaa tcatttctat
    41461 gctgatgagg tcgaactgga attacctcca cgttatcaac atggacaggt cttaatcagc
    41521 aactatgaga aagtttctat ttgtgaaaaa gtgatactga aaccttatca gacacttgct
    41581 atcttagctg ataactaact aaaagcctga ggttaggcct caggcttttg gtgattgtca
    41641 cgaaaaaaga agttttctca tatatttatt tttaaactct tattttttct aaataaaaac
    41701 aattgagaca agttatttta gctaaatttt ggtacaatgg aaaagattat aagtagagtt
    41761 taagataaag attctacaaa tttaaaatga tataaggaga tacgaatgac aacttatcaa
    41821 gatgattttt accaggctgt gaatggaaag tgggctgaaa cagcagttat tccagacgac
    41881 aagcctcgaa caggtggatt ttcagactta gcagatgaaa ttgaggcatt aatgctagac
    41941 actacagatg cttggctagc tggagaaaat atccctgatg atgcaatttt agcaaacttt
    42001 gttaaatttc accgtttggt ggctgactac gctaaacgtg atgaagttgg agtaagtcca
    42061 attctccctt taattgaaga atatcagtcc ttaaaatctt tttcagaatt tgttgctaat
    42121 atagcgaaat atgaattggc aggtttacca aatgaatttc cctttagtgt ggcaccagac
    42181 tttatgaatg ctcagctgaa tgtgctttgg gcagaagcgc caagcattct gttgcctgac
    42241 acgacttatt atgaagaagg taacgaaaag gccgaggaat tacgtggtat ttggcgtcag
    42301 tcgcaagaaa agcttttacc tcaatttggt ttttcaacag aagaaatcaa ggatcttttg
    42361 gataaggtaa ttgagctaga taagcaattg gccaagtatg tcttgtctcg tgaggaaggc
    42421 tcagaatatg ccaaattata ccatccttat gcttgggctg actttaccaa actagctcca
    42481 gagcttcctt tggatagcat ttttgaaaag attttaggcc aagtacctga caaggttatt
    42541 gtccctgaag aacgtttttg gacagaattt gcagccactt actactctga agctaactgg
    42601 gatttactca aagctaattt gattgtggat gcggcgaatg cttacaatgc ttacttgacc
    42661 gatgagattc gtgtacagtc tggtacctat gcgcgtgctt tatctggaac gcctcaagcc
    42721 atggataaac aaaaagcagc cttttatttg gctcaaggac cgtttaacca agcgcttggc
    42781 ttgtggtatg ctggtcaaaa attctcccca gaagctaagg ctgatgtgga aagcaaggtg
    42841 gcacgcatga ttgaggttta taagtctcgt ctagaaacgg ctgattggtt agcaccagcc
    42901 acacgtgaaa aagccattac taaattaaat gtcatcacgc cacatattgg ttatcccgaa
    42961 aaattaccag aaacctatgc caaaaaagtg atagatgaga ctttgtcctt ggttgaaaat
    43021 gctcaaaact tggcaaaaat cacgattgct cacacttgga gtaaatggaa caagccagtt
    43081 gatcgtagtg aatggcatat gccagcccac ttggtcaatg cttattatga tcctcagcaa
    43141 aatcagattg tcttccctgc ggcaatttta caggcaccgt tttattcttt agatcagagt
    43201 tcttcagcca attatggagg aattggtgca gtcattgcgc atgagatttc acatgccttt
    43261 gacaccaacg gtgcttcctt tgatgaacat ggtagcctaa atgattggtg gacacaagaa
    43321 gactatgctg cctttaaaga gcgtacagat aagattgtgg cgcagtttga cggcttagag
    43381 tcacatggtg ctaaggtcaa tggtaaattg acagtttcag aaaacgtcgc tgatctaggt
    43441 ggggtagctt gtgctttaga agcggcacaa tcagaagaag acttctcagc ccgtgacttc
    43501 tttattaact ttgcgacgat ttggcgtatg aaggcccgtg aggaatacat gcaaatgcta
    43561 gctagcattg atgttcatgc cccgggtgaa ctgcgaacaa atatcacctt aaccaacttt
    43621 gatgctttcc atgagacctt tgatattaaa gaaggcgatg ctatgtggag agctccgaaa
    43681 gaccgtgtga ttatttggta attaggtagt tgataaacct tcaaatgcct gtgattataa
    43741 aaatgactga ttctgtgaaa cacaggatca gttttttgct tccttattgg tgtccgtttt
    43801 gatacaacaa aaagacaacc ttcagggaaa gaaggttgcc ttttttagac ttatcagaaa
    43861 taagtgatta gttgttaagg gcagcagcca tagtagctgc aacttctgat tcaaagtcgt
    43921 tagctttttt ctcgatacct tcaccaactt caaaacgtgc aaaagcaatc gctttagcgt
    43981 ttactgagtc aaggtaagct tcaacagttt tgctgtcgtc catgatgtaa acttgtgcaa
    44041 gaagagtgta agcttggtca actttagtgt tgtcaagcat gaaacggtcc attttacctg
    44101 ggatgatttt gtcccagatc ttttctggtt tgccttcagc agcaagttca gctttgatgt
    44161 ctgcttcagc agcagtaatc acgtcgtctg ataattgagc ttttgaaccg tatttcaaga
    44221 atggaagtgc tggtttgtca accattgcac gcgattcgtt gtcaagttcg atagcgtggt
    44281 tcaattgagc aagttcatct ttgatgaatt gtgcatcaag ctcagtgtat gaaagaacag
    44341 ttggtttcat tgcagcgata tgcattgata cttgtttagc aagagcatcg tcgccgcctt
    44401 cgacaactga gataaccccg atacggccac cattatgttg gtaagcaccg aagtgttgct
    44461 catctgtttt ttcaatcaaa gcaaaacgac ggaatgagat tttttctccg atggtagcag
    44521 ttgcattaac gtaagcttca gcaagagttt cacctgaagg cataacaagt gcaagtgctt
    44581 catcgttgtt agctggtttg ccttcagcga taactttagc agttgcgttt accaattcaa
    44641 cgaattgagc atttttagca acaaagtctg tttcagcgtt aacttcaaca acagcagcaa
    44701 cgttaccatg aacgtaaacg cctgttaaac cttcagcggc aacacggtca gcttttttag
    44761 ctgctttagc cattcctttt tcacgaagaa gttcaacggc tttgtccatg tcaccatctg
    44821 tttcaacaag tgctttttta gcgtccataa cgccagcacc agatttttca cgtaattctt
    44881 ttacaagctt agctgtaatt tctgccatgt gtagtatcct ccaaatgata tttgttttgg
    44941 tttcaaaaaa cgagacagag cggtttgctt tctgccccgt ttggttttcg attgttaaag
    45001 acaaacaaag tttgccataa gagttaggaa ctctgtgcta attaagcgtt gtcgccttcg
    45061 acaacttcaa cgatttcttc gattgaatct gcttgagtgt cagcttcaaa agcaacgtct
    45121 gcatcttcac cttgacggcc ttcgataata gcatcagcta atttagcagt gattaattta
    45181 acggcgcgga tagcgtcatc gttagctgga atgatgatat cgatatcatc tggatcagcg
    45241 tttgtatcaa ccatcgctac gactgggata ccaagttttt tagcttcttt aacagcaatt
    45301 tgttctttat gtgggtcaac aacatacatc acgtctggga tacgaggcat atcttcgata
    45361 ccgcccaaga atttttcaag acgagcgcgt tgtttgttaa gaagtgcaac ttctttctta
    45421 ggaagaacgt caaaggttcc ttcttcttcc atacgtttga tttctttcaa acgagcgata
    45481 cgtttttgga tagttcccca gttggtaagc gttccaccca accaacggtg gttgatgaag
    45541 tattggcctg cacgcgttgc ttcgtcagca actgcttcag cagcttgttt tttagtacca
    45601 acgaacaaga tgacagcatc gtttgctgca gcatcacgaa caaattcgta agcttggtca
    45661 gctaatttta cagtttgttg aagatcgata acgtggatac cgttacgctc tgtgaagatg
    45721 tatttagcca tcttagggtt ccagcgacga gtttggtgac caaagtgaac accagcctca
    45781 agaagttgtt tcattgaaat tactgccatg agtagtttct cctttataaa atgttttttc
    45841 ctcttccaga cttcgacttg caggctaacc tagcggcaac aagcccacaa ttggtctaga
    45901 atgagtattt gccgtctaca cgaccctact atattatcaa aaaaagaggt taaaagcaag
    45961 gagaaatact atggattcct ttatttttga gatgactagc ttctttgttt attggtaagt
    46021 gttaagacaa gcttttgctc attttgggtg acacttgcca aatcgtttta tttaagatat
    46081 agtgaatttg actaattttt ggaggcgttt tcatgacgaa gggtattcga tttcaattgc
    46141 ttggcagtcc ccacattttt cttgatgaca aagaacaatt ttttgccttt gccaaagcaa
    46201 atgctctcct ttattactta gtggttaatg gatctgttag ccgcgaggtg gcggcgagtc
    46261 tactttggga aaataagaac atgcaaacag cgaaaaagaa tttacgaaac gctatctacc
    46321 aggtcaataa agtcttgcaa gctgatgtga ttatttgtcc caatcgcaac ctgctggttt
    46381 tgaataaaac acttgatatc aaaacagata ttaacctttt tctggctgat ccgctagctc
    46441 acttagaact ctaccaaggg gagtttttgc agggctttta ccttaaaagt ggtgaggaat
    46501 ttgatctttg ggtttcaaaa atgcgtatgc agtatgaaca ggtataccta aaagcgtgtt
    46561 accaaaaaat tgaagaaaag ctctctttag atgccattga agatgtcgag gaacatttaa
    46621 aacagcttat tgaacgtgat gaatttgaag agaaaaatta tcagctcttg atgaggcttt
    46681 atcagcaggg gaattgtcct ggtaaggtca tcgaaactta ttatcagttg gctaatgtgt
    46741 tggataagga gctagggatt cagccaagtt tgcaaagcca acagatttat caggaagtgg
    46801 tcgctaaaga tcgtaatgaa cgcaaaatca agcatttttt gcgcaacagt aatcattttt
    46861 tggggcggat tgatgagatt aagcagctgg aaaactattt tgccaactgt ttggcctgcc
    46921 aagaggtagg agctctgctc ttaattggtg atacagggat tgggaaacga accctagcca
    46981 gacaggtctt agccaatcaa acccaaacgt ttcaaattgt cacagctaaa tgttttcgtg
    47041 aagaggctat ggattcctta ttgccttggc gtaatatctt agatggcctg ggagatttgg
    47101 tgattcaaaa ccgcttattg actaccaaag cttggaaggc tgctcttaaa cgctgttttc
    47161 ctgtggcaac catttttcaa gaagataaca accaaccctt tatcaaggac cacaccagct
    47221 tgttggtttc ctttattgtt gatattttac aacatttggc agaaatcaag gcgctggtaa
    47281 tccttattga ggattgtcac tggatggatg aggatagttt gaccttgttg caacgggtca
    47341 tgaatcaatt ggtccactat ccgattgctt ttgtcttgac caagcatcta ggaacaactc
    47401 ctgagctcgg tctttgtttg aatgctttga tgagtcaggg gcgtttagaa agcatttgtt
    47461 tggagccttt taatcggcaa gaaagtttgg cttatattaa cagtcaactg ggtagtcagc
    47521 cagtgacggc agaagaaatg gagcacctct atcaagccag ccaaggcaac cctttctttt
    47581 tgtcagaata cactcaggcc ctactgcgtc acgaaaaatt tgtgccttta acgcctgcca
    47641 ttaaggctaa gttgggtctt aaactagcta atctaagtag tcgtgatgac gcattgttaa
    47701 actatttgtc ttgttgtcgg aggcctatcc ctctaaatac cctcgctcaa ttgatgttac
    47761 tgcctttaga agaagtgatc gagatggtgg ataatctggg gcactactat attttggtag
    47821 aagaaagcgt aggagaggaa gtgttgattt catttcgtca gcgaattatt caactctata
    47881 gttatgaccg tttatctttg tcaaaaaggc gtttattgca cggacaaatt gccaaacggt
    47941 tagaagattt actgcctatt ttgacaccaa gccctcactt attagatgac attgcttacc
    48001 attatcagga atcacggcag gtgattaagg ctttggaata caaccttaac tatctagatg
    48061 ctaccttgcc atttcagcat gagctgtttc ccatttattc aaagagtatt ggctccttgg
    48121 aaaagtcaga tcgtgaccat caacgcttaa tggaagagca gtttgataag attcggcaaa
    48181 gcattgcgga tttggaactg acctatgata ataaccgtga tttccagcag ttgcttattc
    48241 gtttttctta ccttgaaggc cgttatgata ttcgaacagg gaggtatcaa gaagggatta
    48301 aacatattca aaaggtcatc gctttggcaa ccgagctaaa acagccctcc ttcttgctag
    48361 aaggctatcg ccagttaatt cattactgta tccaagtgga aaacaagcca gaaatgcgtt
    48421 attacactag tctttcattg gaggcagcgg ttgctgctaa tcattttgag gccattgcga
    48481 ttagtttgcg gttaaacggt ctttatcatt tgattattgg ggagttaaat gaggcagagc
    48541 gtctgcttca acagtcgatt gacttcttta aggtgacgcc aggcttgcaa gctaactatg
    48601 cgattcagat tgctgctgcc ttagattatt tgggggagat tgcccagatt cgctatcagt
    48661 ttgaaaaggc cgttacttac caaaaacaag ccattgcctt gaccgaaaac aagccagctg
    48721 agttatcggt gagtattttt tatattggat tagggatttc ctacttttat ttagctgatt
    48781 ttgaacaagc tgagcaaatc ttgagtttgg ctaaggaagc tttggtcaat catagttatc
    48841 cttggaaaga aacgcaactt gaaatttacc tggccatgat tcaatggaaa aagggtaatt
    48901 atcagccagc cttgacccta cttgattaca gagaaacctt gatgtctagg tatcgcaatc
    48961 cgcgtgataa gggcttggtt ttctatttga tggctgtggt caagtaccaa ttgatatttc
    49021 aaggagctac cctaagtcag caggaaaagg aaatggcaga ccacctgttg tcagaatcct
    49081 ttgagtatta ttatgagatt gctagtacta acctcaaccc ttaccgagat tgtcatttgg
    49141 tgagtgagtt gaacgatttg cgtcaacaat tatctgctaa aagctaagga aaaccctctg
    49201 attcacatca gagggtttta gctatgttgt gccataatct gtacgaggta aaagataaag
    49261 gtggctgcta aattggcggt gtggttatca atatcgtgtg gtggtgaaac ctcaaccaca
    49321 tcaaagccaa ctaattttct actagccgca atgtgttgca aaactaagac ggcaagattt
    49381 gggtcaacac cgagggactg gatagcacta accccaggtg cagcaccaac tgagaaacaa
    49441 tccatgtcga tagtcaggta gaccctttct tgcccttcta agaaacggtc aatggctcgg
    49501 cagacctttt gatgccccat ttggtagatg tcttgaccag tcaaaaattg aatgcccttg
    49561 gattttgcca caaagtcaaa taggaaaagg ttgttattat gttcttgaat gcctaaaacg
    49621 aagtatttaa agaggcgttt gtcagcaacc gcatcgtcaa acatttgtcg aaagcctgtc
    49681 cctgaattag ggcctgtttg gtcgtaaggc cttaaatcaa agtgggcgtc catattgatg
    49741 acagctaggt catcacttgg ggataggctt tggcgtaggc ctaagtagtg gccgtaggcg
    49801 gtttcatggc cacctcctaa gacgatgggt ttgagattga ggtcacacat ccgtttgatg
    49861 gctttgctta aactgttttg cagttgttcc aaagagcgat tggggccgtc aatgttaccc
    49921 acatcgtaaa ccataacttg gtttcctaaa tgccaaggga atttggcgag ctgggttcgt
    49981 atggcagctg gactttcgac cgctccgaca cgaccattat tgatataaac ccccttatca
    50041 cttttaaagc caatcaaggc aaaatgtgtc ccttcaaatg gggttagtga gctgtcgtta
    50101 aggtcaagga aagtcatgac catgccccat ttggcagtat ataagtcatc gtcgatccca
    50161 ccatgataat agctagtggt ggatgggtag taatcttcta gcatgcgcgt catctccttt
    50221 aggattatga tagcaaaata aaacttacag tcaacacttt cttggccttt gtcactggtt
    50281 tttaggaata gcagagcaat aaaatcgtct gcttttggtc ccggttagtt agtctaaaga
    50341 ggtcatgacc tctgccacag cccctctagt taaagaagta ccttattcac taaaattgga
    50401 tactcaactc aactgtctgc tcaaccgctt cgaggaagct tgggtcttca atgactgttg
    50461 atgccttgtt tagttcatcg tagatttcga tgtgtttatc gtgttcgata aagttgactt
    50521 ccttgcggaa gagatcgtag gcaaccttag tccctttacc tagttcatga ttttcagatt
    50581 tgagatcaag ggcttggcag gctgccatga tttcagtagc cacgatgcgg cgagaatttt
    50641 taaggatttc aaaggccttg cgagcagcgg tggtgcccat gctgacaaag tcttcttggt
    50701 tctcacaaga tgggatagaa tccacactgg ctggatgtgc caaaaccttg ttttcagaag
    50761 caagagaggc acaggcgtac tgggtaatca taaagcctga attgagacct gggtatttga
    50821 caaggaagga tggcaatttg ctgagctggc tattgaccaa acgttccacg cggcgttcag
    50881 aaacatttcc aatttcagaa atggcgatgc ctaagaagtc aaatggctgt gccattggtt
    50941 ctccgtggaa gttacctcct gaaataacgt ggccatcttt acagataatt ggattatctg
    51001 tgacagaatt gatttcaatg tcaactttgg atttgacata ggcgatactg tctttgctag
    51061 ccccatgaat ttgagggatg cagcgcaagg tgtaagggtc ttgcacacga gattgagtag
    51121 ctacggttgt gttttggcta ccttctagga ggtttcggat attgcgggct gttgccaact
    51181 ggccgctttg agggcgaatg gtgtgaaggt tttcttcaaa tggactagta atcccgttat
    51241 gcacttcaag agatagggca ccagctaggt ctgacagttt caataattga atggcgtcat
    51301 aggttgcaag ggcaccgatt gcagtgagaa cagttgttcc gttgataagg gcgagtcctt
    51361 ctttagcggc taaagaaatc ttgtcaatac cagctttatc aagagcttct tgaccagaaa
    51421 gaagctctcc tttgtagtaa gctttcccta gaccaagcat aggaagcacc atgtgagcaa
    51481 gcggcgccaa atctcctgaa gcccctagag accctttttc aggaatgtaa ggatgaaccc
    51541 ctttattgag cagttccaac agtttttcaa tggtggatag acgaatacca gaataccctt
    51601 tgacgagaga gttgatacgg atcagcatga tagctctcac ggcatcttct ggcaatgggt
    51661 caccaaatcc actagcatgg gtacgaatca aattttcttg gagttgaact gtatcttctg
    51721 gtgagatgct tacgttacaa agggatccaa aaccggtggt caccccgtaa accacccgtt
    51781 tttcactgac aatgtcatca acaattttac gagaggcatt cacagcttca atagcactat
    51841 catcaatgcg acaggctacg ccttggcgag caatcgcaat cacgtcttcg atagtaaggc
    51901 tttcgccatc taaattaatc actcttgtca tagtttttct cctagatttg ttccttaggg
    51961 ggcatttggt ccatttggtc catttggtgg ttacgtccgt gcataaggta gtacacacta
    52021 cttgcaatga tgacaccaac tgcaccataa acaaagttca ttgggttgtc accccaaatc
    52081 ataactaaac taacgataac tgcaagaatt gggataatag ggccaaatgg tactcggaag
    52141 atcacattag catcgggatc gtccttccgt aatttcataa cggctagtgc tgttggaatg
    52201 tattggaaga agcggaaaac aacactgagt tttgctaagt tttcaaatga gccagtgagt
    52261 aggagtataa tagcaattgc tcctgaaacc aagatagcaa cgagtggcgc gccattttga
    52321 ttttgcttgg caatagcagc tggtaagagc ccttcatcag caatggcagc accgtaacgt
    52381 ggcaccatga tggattctcc catgttaaga cctgtaatgg aaattaaagc accgattgag
    52441 accatccaag cgccagcagg gcctatcatt ttaacaaagg catcttgaac aggtgcattg
    52501 gtcatcatga tttgagagcc aagcatggca atggttccac cgataatcag catatataag
    52561 acagagacga tactgattga tcctaaaaga gcacgcggta catttttttc agggtcacgc
    52621 atttccccag cgacaataga gagggtttca aaaccgataa agccatagaa gatataaaca
    52681 gcagtattgg agatagcccc taagaggtta gtccctggtt ccaactgaac aaatggggta
    52741 aagttaggaa gcccgttttt gatgaagaaa agggtacaga cacaaaaggc aacgattgga
    52801 attaacttag caatggtggc tgtgatggtg acaattttag aggttttcaa accagcgata
    52861 ttcatcaaag atagtaagat gattaaccca atactaagtg gaatgtgcca accttcaaag
    52921 gcagggaaag taatgataaa cattctggca aacccagctg ccatggctgc ccaagcgaag
    52981 atagtaacgg tccaaccaag gaagccaacg ttaaagccaa taaaatcacc aaaagctcgt
    53041 ttggaatatt ggaaggctcc gccgtttttc ccaaaataac cagagacttc tgcaaaacaa
    53101 actgccaaca tgatggtcaa aatagctgtt ccaaacataa cagcgatgga tgcagggcca
    53161 aggcccttgt aaatggccct aggtaagaga aaaataccag agccgataac ggcattgatc
    53221 ccgtaaagag tggcaccgct caagctaaac tttgcctgtt ctctttcttg ttcgttcatg
    53281 tgatttgacg ccataataat gctctccttt ttgtttgcaa attgcgttca aatataatgg
    53341 ataaggtcgc taggaaatga ggagatagtc ggtttaggag ggtccttctt gcacctaacg
    53401 atagtgatga gctattggtt agcgatgaga aataggtagc cttttgtgat gatgtgtagt
    53461 gatttcattg gagaagaaca ggcatgagta gtgtgatgcc tatctttatc ccactaagtc
    53521 ttataccata agcctgccca aacagctact ggagtttggg cggggcttgt ttgattgggg
    53581 caaactttta ggtctgaaag gctggcctcc tgctcgatta ccaacaagtg attggcttca
    53641 agctcttgat tcgcctgatt ggtcacagaa taagtcagtt tgcttagggc ataaagcagt
    53701 tgacagtccg ctttaggtgt ctcaaatagt gtgtcagacc agacgggtaa taattgccct
    53761 tggtattggt catcatacat taaattgatg tcttggcaga ttccacgact gacgattctg
    53821 gtatcgcctt caaacgcata gggttcaaaa ggagttaggg aaatctcttt agcatgactc
    53881 aaatcactca atgtgatggg cttgtctagg gtcattagga tcctgtgata gccatcaagt
    53941 aaggtaaagg ttgtctgtcg tagggccaca gttgactttg agaggcgata agagaattct
    54001 ctctttttgt aatctccatc ttcgggataa aggaacaatt gattggtttc accgccagac
    54061 cagtcagttt gagtgtaatc tgctggcgtt ttgaccatta cggttgtcat ctgttctctc
    54121 cttgtttcca attagaatag tcctgtgatg ttgccctcct cgtcaatatc aattgtttcg
    54181 ctggcaggta cttttggaag gccaggcatg gtcatgatag cacccgtcag agcaacgata
    54241 aatcctgctc ctgcggacac tttaaggttg ctaatagtca ctataaagtc agttggtgcg
    54301 ccaagttttt tggcatcgtc tgagaaggaa tactgggttt tggccatgca aattgggtag
    54361 ttgccaaagc cgaggcgttc taattctgcc agttcacgtt tggctgtggg ggtcaatttg
    54421 atacccttac caccgtaaac cttggtaacg attttagtca gtttggtttc aatgctatcg
    54481 tcttcattgt aaacaaagcg gaattgattg tcttgttctg ccaatgctac tactttttca
    54541 gccagttcac ggccaccagc tccgccattc gcccaaacgt cagaaatcac aacatcaacg
    54601 ccacgtttgt cgcaggcatc atagacagct tgtaattcgg catcggtatc aagcgggaat
    54661 ttattgatcg ccacgacaac tggaagacca tagacgtctt ggatattagc caaatgtttg
    54721 tcaaggttag gtaaaccatc cacaacggcc tgaacatttt cagtagccag gtctgctttt
    54781 ggcacacccc catgcatttt aagggcacga atagtggcta ctaggacaac ggctgctgga
    54841 cgaaggcctg acatgcggca tttgatgtca atgaattttt cagctccaag gtcagcacca
    54901 aatccagctt cggtaacggc ataatcgcca tatttcaagg ctagtttggt ggctaggaca
    54961 ctattacagc catgggcgat attagcaaat gggccaccgt gaatgagggc tggtgtgtgt
    55021 tccagggttt gcacgagatt aggatgaatc gcatccttga gcagtgctgc tagggcacca
    55081 ccagctttca agtctttagc tgttacaggt tcaccttgga agttgtaacc gatgataatt
    55141 ttttcaaggt gggctttgag gtcagaaata ttttcggata ggcacaggat tgccatgatt
    55201 tcagaggcga cggtaatgtc ataaccatct tcacgaggga ccccgttgac cttaccttgc
    55261 aagccgtcaa caatatgacg aagctggcgg tcattcatat ccaccacacg tttccaagtg
    55321 atacgtctag aatcaatgcc taggctattg ccatggtgaa tgtggttatc aatcaaggca
    55381 gctaataagt tgttggcgac accgatggca tgaaagtccc ctgtaaagtg aaggttgatg
    55441 tcttccattg ggacaacttg cgcgtggcca ccgccagcag ctcctccctt aaccccaaaa
    55501 acaggaccaa gagaaggttc acgaagggca ataactgctt tttttccaat ggcagaaaga
    55561 gcatctacca atccaacaga agtggtggtt ttgccttcac cagctggtgt tggtgagata
    55621 gcagtgacaa gaatgagttt gccatcaggt ttgtttttta aggcgaccag ttgacgggca
    55681 tcaatttttg ccttgtactt accatagaga cagagcgctt ccttgtcgat ccctaattga
    55741 tcggcaacct tactgatagg ttccatagtg acagagttgg ctatttcaat atctgataaa
    55801 accatagttg ctcctttact tgttaaacaa tatcgagaat tttcgtgtag atgtcatctg
    55861 ccaaatgaca gcccttgtct aataaggctt gtcctttttg gcgatagtcg gtaacaaagt
    55921 cttcatcttt gatgccagac agattaatca aaacattcaa ccaagcgcct tgtaaacctg
    55981 cttttaagtt taaagcagca actcctaagt cactagcagc atttgtatta gacttaccga
    56041 cagctgttgc agtgatctca agtgcttcga ccatgagggt catcatctca aaaggtgatt
    56101 gtgcagctgt tttcaaagcc ttttgcatgg cagttcgtct ggcagccttg tcttcgtcag
    56161 tctcttttgg catgtcgaaa acgtcagaaa ccaggttgaa ggcttcggtg tctttgtcaa
    56221 tggcatctaa taagtcctgt tgcaagcgac tacttttggt atgaaggtct tcaatgatat
    56281 cttgataatc agcatacttt ttcttaccaa gggtcaattc acagaccatc ttggtcaagg
    56341 aaatgccatt agcgccagag agagcagcag cagaaccacc acccggagca ggggcatctg
    56401 agccaaggac tttagcgaaa tctgttaagc ttaaatcaac taatcccata agacaccccc
    56461 tagcctagca aatggttttc aagaatctgt ttatggtagt caaagtcctc aacttgcaag
    56521 tagtattcag ccacatcgat taaggcttta gctggcgcta agcctatgat ttcagaacca
    56581 ataacattca caccgtagcg acgcgcttca aatttgatgg tttcaaaagt acggtaaaga
    56641 gaacattttt caaagttaac catgttcatg gaaacttggg caatatgacg gtcttctaac
    56701 atgacaccga tagccttaca atatttgtag ccaccacctg aaccgcggat aattttcgca
    56761 attttatgag caatgtcgat attgtcagta tccaaattaa cattgaaagc aaccaatggc
    56821 atacgagcac caacggcagt tatcccagca gttgggtgaa tcttacgatc tccgtaatca
    56881 ggtgcccaat cctcttccaa cagtttttct ggcatgcctt cgaattgtcc tttacggact
    56941 ttggccaggt tttgacgttc tgggcgtgtg gcagaatctt cataaaggaa gattgggatt
    57001 ccaagttcac ggttaatccg ttctgcgact tgtttggaaa tctcaacaca ttcttgtgta
    57061 gtgatgtctt tgatcggaac gaatgggcag acatcggttg ctcccatacg agggtgttca
    57121 ccatgatgtt tggtcatatc aatgttttca gaagcgtatt tcaccagttg gaaagctgct
    57181 tcttgaatag actggtcatc tccaaccaag gtaaacacgc tccggttgtg gctcgcatca
    57241 gaagagtagt cgagaagggt cactccagga atacttttgg ccgtcgctac caaaccatcg
    57301 ataacagcct gattttggcc ttctgagaag ttgggaatac attcaacaat tttcgccatg
    57361 ttaatctcct tttatgtcat tgagttactc attaagggaa taggttacct actttagata
    57421 acgcttacat ttttagctag cagttattag tgaaataatt gatctaccgc tgaagtcaca
    57481 aggtcatcat cagccaagta tgggatagtg atgtgatcag tacctgcatg gagacggttg
    57541 tattcaatag cggtttcaat agcgtgctca ttgcgagccc aattgcgacg agcaacacca
    57601 cccatggtgt cccaagcaat agcagattta ataatctcat caatgcgttc gctgccatct
    57661 agaacaagtc caaagccgcc gttaatggct ttaccgatac cagttccgcc accgttatga
    57721 agagccacta aactcatacc acgcgcagcg ttaccagcgt aacattgaac tgccatatca
    57781 caggtgacat tggaaccgtc tttaatgttt gaggtttcac ggaatggtga gtcagtacca
    57841 gacacgtcat ggtggtcacg gccaatcatg acaggtccaa tcttaccttc tcggaccaat
    57901 tcattaaatt taagggcgat agtcacacga ccaatacagt cttggtaaag gatacgggct
    57961 tgtgtgccga cgaccaattg atttttctca gcatcacgaa tccagttata gttatcacgg
    58021 tcttgatagc ggcggtcagg atcgatagct tccatggcag ctttgtcagt agccaccaag
    58081 tcttcgtgtt taccactcaa gcagacccaa cggaaaggtc catagccata gtcaaaaagc
    58141 ataggtccca tgatatcttc cacataggat ggccagataa agccgtcttt gtcgttacga
    58201 ccattcttag aaatttctgt aataccagag tcgtaaacag acttcataaa ggcattgccg
    58261 tagtcaaaga aataggtgcc attttcagtt aacgtcttaa tagcttcaaa atgccgtgcc
    58321 aaggtatcat caaccatttg gtggaaggta tccttgtctt ctgccaggag ccgggttctt
    58381 tcgtcaaagc taatacctac tgggcagtag ccaccgtcat aaacgttgtg acaagaggtt
    58441 tggtcagaca aaagatcaac atgaatctgc ttgtcattga cgtactctaa aaggtcaaca
    58501 atattaccat ggtaagcaat cgaagtggac tccttagcat ctatggcttt tcgggctaat
    58561 tgcagagctt cttcagggct ctcagcaatt tggctaaccc agccttgcga atgacgggtt
    58621 tcaatccgag attggtcaac ttcagcaata atggcaactg atttagcaat ttcagcagcc
    58681 ttaccttgtg ccccactcat gcctcctaaa ccagatgaga taaagagttt accggtcaaa
    58741 tcaccatcgt cagccactcc aagtttgaga cgaccagcat ttaagagagt attaaatgtt
    58801 ccatgaacaa tcccttgtgg gccaatatac atccaaccac ctgcggtcat ttgaccatag
    58861 ttggtgacac ccatctcttc ggcgatttcc cagtctttca tgttgtcgta ttcacccacc
    58921 aaaaggccat tagtaataat cacacgaggc gcttcaggtt tggatttgaa aagaccaact
    58981 ggatgaccag actcaaccac caaggtttgt tcatcagtca tgacctctag gtatttttta
    59041 atcaaacaat attgcatcca gttggcacaa acagaaccag tttctccata agtgactagt
    59101 tcgtaagggt acaaggcaat ttcaaaactc aagttattgt caatcatgac ctgcatggcc
    59161 ttggcagctg tacaatttcc cttgtactcg tcaatgggtt tgccgtaaat gcggtctttt
    59221 ggacgccatc tgtaaccata gatgcgtccc cttgttttga gttcttccaa aaattctgga
    59281 atgacttctt cgtggaactt cgtaggtaca tagcgtaggg cattttttaa ggcaatttcg
    59341 gtctgagcct gggtcaatcg aaaacctctg tctggtgatc ttcggatacc ttctttgaac
    59401 acagtctttt ccggtaaaac atcgtctaac ttaacagtca tagcggcagc tatgtcagtt
    59461 tcactgtaaa atgacataga aaactcctcc tcaactcctt tttattgtcg agattagtct
    59521 aacttagtct agtctataaa aagtcaacca cgctttgaaa tgcgaaaaaa gtctaaaaaa
    59581 aaatcaaaac ctcatttttt gactaaaatt tgacgcttaa tcagtacact tgaggtatga
    59641 cagataaagt cagtcagagg ttaattgttg ttaccaaagt tgcagagatc gcaactattg
    59701 agtgggtgag gggggctagt aatggctatt aatcagttga caaacgatgt taaaaaagga
    59761 ggtttttatg gttgctgatg tcttattaac acattttaat caactatttt gtctaaatga
    59821 tccaggtcat cctttaactg gtcaagaaat gaaaaaagcc actatcgttg aagatggcta
    59881 tatagccatc aaagacggcc tcattgtggc tcttggttct ggtgaaccag atgctgagct
    59941 tgtcggtcca cagactatta tgcgttctta taaaggtaaa attgcaacgc ctggtattat
    60001 tgactgtcat acccatttgg tctatggtgg cagtcgtgaa catgaatttg ccaagaaatt
    60061 agcaggggtg tcttacttgg atattttagc tcaaggtggt ggtattttaa gcacggtgag
    60121 ggccactcgg tcagccagct ttgacaacct ttatcaaaaa tccaagagat tgctagacta
    60181 catgttgctt catggagtga ctactgtcga agcgaaaagt ggctacggcc ttgattggga
    60241 aacagaaaaa cgccaactgg atgttgttgc tgctttagaa aaagaccatc ctattgattt
    60301 ggtgtctacg tttatggcag cccatgcgat tcctgaagaa tacaagggga atcctaaggc
    60361 ttatctcgat gtgattatca aggatatgct tcctgttgtt aaagaggaaa atctggcaga
    60421 attttgtgat attttctgtg aaaaaaatgt ttttacagct gatgagtccc gctatctgtt
    60481 aagcaaagcc aaagaaatgg gcttcaagct tcgtattcac gcagatgaaa ttgcgtctat
    60541 aggcggagtt gatgtcgcag cagagctcag cgcagttagt gcagagcatt tgatgatgat
    60601 aacaaatgat gatatcgcta agttgattgg tgctggtgtt attggtaatt tgcttccagc
    60661 caccaccttt agtttgatgg aagacactta tgcccctgct cgcaaaatga tcgatgctgg
    60721 catggccatc accctctcaa cagatagtaa tccaggcagt tgcccgactg ctaacatgca
    60781 gtttgtgatg caattaggtt gttttatgtt acgtttaaca ccgattgaag tcttaaatgc
    60841 tgttaccatt aacgcggcct actctgtcaa tcgtcaggag agagttggta gtttgacagt
    60901 tggtaaagaa gcagatattg ccatttttga tgccccaaat attgattacc cattttattt
    60961 ctttgccacc aacttgattc accaagtcta taaaaaagga caactcacgg ttgatcgagg
    61021 tcgtattctt taaaaaattt caaaaagaag ctttgagcgt tagctcaaaa cttcttttaa
    61081 atatgctatt ttattgacga atcaagtagt caaaggcacc gatggcagca gtagcaccag
    61141 accccatgga aataatgatt tgtttataag ctgaatcagt acagtcccca gcagcaaaga
    61201 tgccgggaat attggttgaa ccatgtttat ccacaatgat ttctccgcgg tcggttaggt
    61261 tgacaccact atctttgagc caagctgtgt ttggaacaag accaatttga acaaagaccc
    61321 cttcaaggtc aaggtgtttg tcttcaccgc tatcacgttc agtgtagttg agaccagtaa
    61381 catgatcgtc accaacaatg tctttagtag caacattttt gataatagtc atgttagcag
    61441 tatcagctgc gcggtcttga agcactttgt cagctttgag ctcaggcaag aattctaaca
    61501 cgtaaacgtg tttagcaaga ccagccaagt caagggccgc ttctaaaccg gagttaccac
    61561 caccgataac agcaacatct ttgccttcaa agagtgggcc atcacaatga ggacagtaag
    61621 tcaccccttt attacggaat tcatcttcac caggaacatt aatgttacgc catttggccc
    61681 caagagctaa gatagcagtt ttggcttgta aaaccgcacc attagctaga gtaacctcga
    61741 tgttttcttt tttctcaata gaagtagcta gttgtgcttt gataatatca acatcataag
    61801 acttagtatg ggcctccact tcggccatta attttggtcc ttcagtgtaa agggtaccaa
    61861 tcatgttttc gatgccgacc gtttccatga cctgaccacc aaaggtttca gcaagtaaac
    61921 ctgtttttag tcctttacga gctgcataga tagcagcgct attgcccgca gggccaccac
    61981 caataacaag gacatcatag aggcctttat cagcaaaggc ttcctcagaa agtgggccag
    62041 cgatttgttc taagagttgc tcaatggtag cacgaccaga agtgaattct tccccgtcta
    62101 aaaagacagt agggacagac ataatgcctt tggctttcac ttcatcttgg aacatgccgc
    62161 cttctaccat agtgtgtgaa atcttgtcgt tcaagacgga cataatgttt aaagcttgaa
    62221 caacatctgg gcagttatgg caggttaagc tgacataagt ttcaaaatgc aacggacggt
    62281 caatggcctt gatgcgatca ataacatctt gatccacttt tggagcacgt ccagatactt
    62341 gcaagagagc aagaataaag gacgtcaatt cgtggcctaa aggaagacca gcaaagacaa
    62401 ccccactatc gtgacctttt ttcgcaacct taaagcttgg ttgtcggtct aaagtgatgt
    62461 tttcaataga aatacgctca gacatagcag ctatttcttc cacaaagtct ttgacttttt
    62521 gagactgctc attgtcacca agggaaactt gcaaaacaag atcagcttct aacagggtga
    62581 ggtattgggc aagttgttct ttaatatcag gacttaatgc catagctttc ctcctaaaat
    62641 ttattaaatt ttaccaacta aatcaagact tggtgtcaat gtttcagcgc cttctttcca
    62701 tttagctgga caaacttcac ctggatgttt acggacgtat tgggcagcgt gaattttatc
    62761 aatcaaggtg ctagcgtcac gtccaatacc atcagcatta atttccatca tttggataat
    62821 accatctggg tcaacgataa atgttccacg ttgagcaagt ccgtcttcgc caagcacttc
    62881 aaaggcttgt gaaataaggt gtgaagggtc accaatcata gggtatgtga tagtgccaac
    62941 cacatctgaa tcatcatgcc aagctttatg aacaaaatga gtatcagtag agacagaata
    63001 aacttctaca ccaagagatt tcagtgtttc gtattgctct tgaaggtcac cgagttcagt
    63061 tgggcaaaca aatgaaaagt ctgctgggta gaaacaaaaa actgcccatt ttcctttaac
    63121 gtcttcattt gtaacagtga tgaattttcc atcgtgataa gcttgagctg aaaattcagc
    63181 aatttctttt ccaattagag acatatagtt gtcctccttt tttatagttt tatacttgta
    63241 ttataaaaga acaacaagat tatgtctact cagaaaccct tgattttact gggaaatcag
    63301 cgattgtttt ctgacaattc agttgtgaaa taaatcacat taattaaaat gattctaaat
    63361 aagctgaaga cgccgttaaa tcaaaggtta atttaattat aatctacaga aaatataatt
    63421 gttattaaag cgtttacaaa aaactttttt gattccgtta aggcaactta atattatggt
    63481 tacctaatca ctaatattgt aacctatttt ggcatgagat cagggaatgg ctgttatatt
    63541 aattggatat gtcttgtgca aatagtgctt gacggatttg tggaattagg gtaaaataat
    63601 ctttgttgtg gcggtatagc caagtggtaa ggcacggctc tgcaaaagct tgatcgtcgg
    63661 ttcaaatccg tctaccgcct tttatataca aaacaagcct tgattaactt cgtgaaaacg
    63721 ttgttaaatc aaggttttta ttttttgcga caggataaaa aaagagaaaa aaagaaataa
    63781 agtcggtcaa aagtcggtca aatttcccga agatgtcttt tatagcctga ttattaggta
    63841 tgaaaaatac tatggccaaa attggtaaac gattgcaaat gaaggtcaga gttcttaccc
    63901 ccttaaacga tatgcgttaa aagttagttg aactgccgtg cacctaacga tagacacggt
    63961 gatgtgagag cgagtagagt tgaatctcta ctcatttatc ttaccatagt cagctttata
    64021 aagtaaccac ccattacaat gcaactatat tcttaatgtt tttaagcatg ttattgacaa
    64081 gtgttataaa atgtgttact cttagtgagt aaattagttt ttgagttagt atttgtgttt
    64141 caacatcttt atttctctct atttttacaa atgattttta gaaaggaagt ggatgtatat
    64201 ggcacaaggt acagtaaaat ggtttaacgc tgaaaaaggt tttggtttta tttcaactga
    64261 aaacggtcaa gatgtctttg cacatttttc agcaatccaa actaatggtt ttaaaacgtt
    64321 agaagaagga caaaaagtgg catttgacgt cgaagaaggt caacgtggac ctcaagcagt
    64381 caatatcact aaattggcct aatctggtta aagtaactaa ggagaagcag atgttggaaa
    64441 caacaactgc ttttttgatt tctaccagtt acctttaatg attaagagtt gcgtttaagg
    64501 tcttgtattt gttataataa tagtcagttt tagtcaaaat gtattgacgt ggtgctacta
    64561 agtttttaga gtagcccgca agcaaaggag tgacagcatg ccaaccaaaa atacttcaga
    64621 cagcattgaa gagtatatta aagaattatt agctaagtca ggaatagcag aaattaagcg
    64681 gtccatgcta gcagattcgt ttcaagttgt cccaagtcag atcaactatg tcattaaaac
    64741 ccgttttacg gagagtcgag gctatgaagt agagagtaaa cgaggtggtg gaggctatat
    64801 tcgtattgcc aaggttcatt tttcagacaa acaccatctg attggcaatt taatggcaac
    64861 tattgaagac tgtatcagtg agcaagtttt cacggactcg atacaattgc tttttgacga
    64921 acacttattg acagagcgtg agggaaatat cattttggct atggcgtctg atgatgtttt
    64981 aggaactgat ggttcaacta ttcgtgcacg aatgctctat cgcttattgc aacgaattga
    65041 cagaaaggga agcaattaat gattatgtat tcaacgaaga tgcaagacat ttttagacag
    65101 gcgcagttcc aagctgctcg ctttgatagc cattgcctgg aaacttggca tgttttgtta
    65161 gctatggtag ctgtagataa ttctttagca aatatgattt taagtgaata tgatgcccaa
    65221 gtcgccatag aagaatatga agctgcagct attttagcca tgggcaaaac ccctaaggaa
    65281 cagttgtctc gtgtagactt cagacctcaa tctaaaacct tgactaactt gttagctttt
    65341 gcgcaggcta ttagccaaat cactagggat caagaagtcg gctctgagca tgtcttattt
    65401 gctattttat tgaatccaga tatcatggcg agtcgtttgt tagaaatagc aggctatcag
    65461 ataaaagata acggcaatgg gcagccgcga ttagctgact tgcgaaaagc aatagaacgt
    65521 catgcaggtt acagtaagga aatgattaag gctattcacg aactacgtaa gcctaaaaaa
    65581 acgaaaacac aagggacctt ttcagatatg atgaagccac caagtacagc tggtgagttg
    65641 agtgatttca caagagattt gactgaaatg gcaagacaag gtttgttaga atcggtgatt
    65701 ggacgtgacc aagaagtatc tcgtatgatt caggtactaa gtcgtaaaac gaaaaacaat
    65761 cctgtcttgg taggtgatgc aggtgttggt aaaactgcgc ttgcttatgg ccttgctcaa
    65821 cggattgcaa atggcgctat tccttatgaa cttaaggaga tgcgtgtcct agaattagac
    65881 atgatgagtg tggtagcagg aacccgtttt cgtggggatt ttgaagagcg catgaatcaa
    65941 atcattgatg atattgaagc tgatggtcag attattcttt ttgttgatga actacatact
    66001 attatgggat ctggcagtgg tattgacagt acacttgatg cggctaacat tttaaaacca
    66061 gcattatcgc gcggcactct tcatatggtt ggagcaacaa ctcaagaaga atatcaaaaa
    66121 catattgaaa aagatgcagc tctttcgcgt cgttttgcta aaatattaat tgaagaacct
    66181 aatacagaag atgcttatca gattttgatg ggcctaaaat tatcttatga gacctaccat
    66241 aatgtctcga tatcaaatga ggcagttaaa acagctgtaa aaatggcaca ccgttattta
    66301 accagtaaaa atctccctga ttcagctatc gatttattag atgaagctag tgctgctgtg
    66361 caaaacatgg tgaaaaaatc agcacctgag actttaacac caatagacca agctcttatc
    66421 aatggtgata tgaaaaaagt atctcgcctc ttagctaaag aagcaaaagg tcagatgaga
    66481 aaaccaacac cagtcacaga agatgatatt ttggcaacct tgagtaagtt atcgggaatt
    66541 ccacttgaaa aactgacgca agctgatagc aaaaaatacc tcaatttaga aaaagaactg
    66601 cataagcgtg tgattggtca ggatgctgct gttacggcta tttcaagagc cattcgtcgc
    66661 aatcagtcag gtattcgaac aggaaaacgt cctattggat catttatgtt tcttggccca
    66721 acaggagtag gtaagacaga actagcaaag gcccttgcag aagttctctt tgatgatgaa
    66781 gcagcgctta ttcgttttga tatgtctgag tacatggaaa aattcgcagc gtctaggctt
    66841 aatggagcac ctcctggtta tgtcggctat gatgaaggag gtgaactgac acagaaagtt
    66901 agaaataaac cttattcagt cttgcttttt gatgaagtgg aaaaagcaca tcctgatatt
    66961 tttaacgttc tccttcaagt attagatgat ggtatattga ctgatagtcg tgggcgtaag
    67021 gtcgattttt caaatactat tattatcatg accagcaatc ttggcgcaac agccctgcgt
    67081 gatgataaaa cggtcggttt tggggtcaaa ggcattcacc aagaccatca agctatggag
    67141 aaacgtatct tagaagaatt aagaaaaact taccgcccag aatttatcaa tcgtattgat
    67201 gaaaaagtgg tctttcatag tctgacccaa gataacatgc gcgatgtggt taaaatcatg
    67261 gtacagcccc tgattactac attggcagaa aaaggtatta cccttaaaat tcagcctttg
    67321 gccttgaaac atttgtccga ggtcggctat gatgaacata tgggggcaag accattacgt
    67381 cgaacgctgc aaactgagat agaagataag ctatcagagc ttatcctttc tcgagaattg
    67441 acaagtgggc atacgctaaa aattggatta tcacatggca aattaacgtt tcacatagct
    67501 taatttccat ataaaaggtg gatcatcctt gatgtcacca aaaaaagagg ttattcttac
    67561 aaaaataact tcttttttct tgactatttt tgaccaagtg ataaaataga atataaagtt
    67621 agcactcgat gaacatgagt gctaacttta ctaaagattc ttttattgga ggaaaatttc
    67681 atgttaaaac cattaggtga ccgtgtggtc gtaagatttg atgatgaaaa agagcagaca
    67741 gtcggtggtt ttgtccttgc aggcactcat aaagagtcaa cacgaaaagc gacagtcctc
    67801 gctgtcagtg aaacgggtgt tcgcactatt acaggcgata gcgtgctacc ttcagtttca
    67861 gtgggtcaag aggttttagt tgaaaatggg catgatttag aagtaaccgt agatgatgag
    67921 aaagtttcta tcatccgtga atctgacatc attgctattg tcacaaaata aaacgacaaa
    67981 tctctaaata gaaagaagga ttgaatatgg caaaagatat taaattttca gcagatgcgc
    68041 gtgctgccat ggtgcgcgga gttgatatgt tagcagatac cgtcaaagta acgcttggtc
    68101 ctaaaggacg caatgttgtt cttgaaaaag cttttggttc tcccttaatt actaatgacg
    68161 gggtaaccat tgctaaagag atcgaattag aagatcattt tgaaaacatg ggagcaaaat
    68221 tggtgtctga agtggcttct aaaaccaatg atattgctgg tgatgggacg actactgcaa
    68281 cagttttgac acaagccatt gttcatgaag gactaaaaaa tgtgacagca ggtgctaatc
    68341 caattggtat ccgtcgaggc attgaaacag caacagcaac agctgttgaa gccttgaaag
    68401 ccattgctca acctgtatct ggcaaggaag ctattgctca ggtcgctgca gtatcatcac
    68461 gctctgaaaa agttggagag tatatctcag aagctatgga gcgtgtgggc aacgatggtg
    68521 tgattaccat cgaagaatct cgaggtatgg aaacagaact tgaagtggtt gaaggcatgc
    68581 aatttgaccg tggttacctg tctcaataca tggtcacaga caatgaaaaa atggttgcag
    68641 accttgaaaa cccatttatc ttaatcacgg ataaaaaagt gtcaaacatc caagatattt
    68701 tgccactact tgaggaagtt cttaaaacca accgtccatt actcattatt gcagatgatg
    68761 tggatggtga agcccttcca acccttgtct tgaacaagat tcgtggtact ttcaatgtgg
    68821 ttgctgtcaa agcgccagga tttggtgatc gccgtaaagc tatgcttgaa gacattgcta
    68881 tcttgacagg tggtacagtg attacagagg atctaggact tgaattaaaa gatgctacaa
    68941 tgacagccct tggacaggct gctaagatta cagttgataa agatagcaca gtaattgttg
    69001 aaggttcagg aagttcagaa gctattgcta accgtattgc actgattaaa tcgcaattag
    69061 aaacaacaac ttctgacttt gaccgtgaaa aactacaaga acgtttggcg aaattagctg
    69121 gtggtgtagc tgttatcaaa gtaggagctc caacagagac agctttaaaa gaaatgaaac
    69181 ttcgcattga ggacgctcta aatgctacac gtgcagccgt tgaagaaggt atcgttgctg
    69241 gtggtggaac agcacttatt acggttattg aaaaagtagc agctcttgaa cttgagggcg
    69301 atgatgctac tggacgtaac attgtgcttc gtgctctaga agagcctgta cgtcaaattg
    69361 ctttaaatgc tgggtacgaa ggctccgtag ttattgacaa gttgaaaaac agccctgcag
    69421 gaacaggatt taatgctgca acaggtgagt gggttgatat gattaaaaca ggaatcattg
    69481 accctgtcaa agtaacacga tcagcgcttc aaaatgcagc ttctgtagct agtcttattt
    69541 tgacaacaga agcagttgtc gctaataaac ctgaaccagc tgcgccagcg ccagcaatgc
    69601 cagcaggtat ggatccagga atgatgggcg gcttctaagg tctctatagc attaaggatt
    69661 atgaaaaaga agttgagact aaaggtctgc ttcttactat gaaaaaagaa caaagattgg
    69721 atttcgactc atgatatccc atctttgttt ttttagttgt gatataaaat gctaatcttt
    69781 gtggttacag acttttaatg tattggaaaa tacattgttt ttctttgtca tgtgctatca
    69841 tggagtcaat gaaaagttat tcccatatat gggtgttgga ggtctgtgat gaatcatttt
    69901 ggagagattt taaaaacctt tagagaatca aagggattac gattaaaaga tgtcgcaaag
    69961 gctggtatat caacctctca gctgtcgcgt ttcgaaaaag gggagacgga cttaaccata
    70021 tcgacattta tgctgatttt ggatgaaagt aacatgccca ttgatgagtt tatgtatgct
    70081 gtccatgatt ttcatcgtga cgatttaaat gaactcttat caaaaagtga ggcatttcgt
    70141 aacaactcaa gatagtgatg gcttgaagca gttacttcat gctcaactag cataaactga
    70201 taagaaggag atgttcacgg atatcttttg gcatatccag catgtctaag tctgctatgg
    70261 catgctattt tgggagtccc tcacgcgact ctgtcagtag ctctccttca aaggattcac
    70321 atagaaagtc gtaatagaca tagcagtcag gcttgataaa gctagaaata ctctttaatt
    70381 gaaggtttaa agctgtcaga cctatctctt cttttagttc tcataaagcc gcttcaaaga
    70441 aagattcaca gggctcgact ttaccttctg gctggaccca tcctgggaag ttatcgtgtt
    70501 gtctatggag tcataaaatc atatcgttat gtttgacaca catattgccc cagtctctaa
    70561 ccgttgttat caccagattc accacctttt agtatagtat aagaaaaaag gggtaaaaga
    70621 ggtttcttgt tgcttttttg gcttaaggtt tctttaagtc ttatctagta tactaatgtc
    70681 attccttaat aggataagta tttggttgta gaagtatcgt cttaaactaa cgctatctca
    70741 acccgattcc catcttattc acccctccta taccgataag gtctcacaaa gtgtgagacc
    70801 ttattttttg acttcagctc tagttatgga gaaacttgtt gatacaagct tttttgggtt
    70861 atggtattat gatctattca agcctttctt gtttttgggt ttttctttaa ccaacttggc
    70921 ttcaatagct tttaagcgtt tgaaggtttt ttcagcgcga tctagtgaaa tcttatcagc
    70981 tttttcttga gcagctttgg ggtcactgac gactaagtca ctgatttctt tggtgctttt
    71041 gtcttgttcg gctatccagg ttttttccaa gcctttcatc tcatcaatca ctttagttcc
    71101 aaatggtttt ggatgagcag ccacgagatc attaatgtgt gacaccgtcc aataccatga
    71161 cttatcgtta tattgggtgc ttttttcttg ataggcttca taggttctac tgatattgcc
    71221 aaggtatggc aagtaagggg cgttacgagg actgccaata gatagccaca tgacaccacc
    71281 acccaattca gcagggatgt cttttttcag ttggaaaata tgagcttcca tgacattagg
    71341 atttgagatg ggataagcat aacctttaac agcttttttg gattttggtt tacccttacc
    71401 gtctaatgcc atctgatcaa gtggtttaag atccagcccc tcaaaacgat tacgttgcag
    71461 tttcatcgca tcttctagac tgaaggtctt atcagtgctt tggagcaact cataattgct
    71521 gtctttataa gttacttttg aatcagggtc aagtgattta atccctgaga aagagcgaga
    71581 gcggttggca tcgtttagtg gcggattgta agatttggca atatgaaatt taccatcaac
    71641 ttcgatataa gatttggctt ttttagcgac tttttccaca tcttctgagg caatcgtatt
    71701 ttccttatcg ttgaaatcga catgtccgag atagaaggta tttggaaaaa cagcgtattt
    71761 atcatctggg aatttgatgg caacgtattg gtgaccagag agaatttcca tgtaccagat
    71821 gccgtctttg tcagccaagg taacaatatt tccttcagca gctccttttt cagtgactat
    71881 tttagcaatc aatgcgacac cttcacgggc tattttgacg cttggtaaaa tgacactggc
    71941 catagatgat tccgctaagc catttttgac gtaaggatca attttttgga tggcatcatt
    72001 agctgaagct gatactgttg cagacataga gacgccaaac tcattaaaac cagcttcgtc
    72061 ataaacgcct ttgttaggtg ttacatcagg aatcgcacta tagcgataag aatgctcagg
    72121 caacgggtac tcaaaaccat tagagaggtc tttccatttt tcgccagcag gattgtcctt
    72181 tgctagacgt acaatgaaat ttttattatg atgtggctca agatcttctg ttcgaccata
    72241 aagaagagaa ccgtctttgg ttaaatcttt gccaataata aaaccagtac aagcataact
    72301 aacactttga agcgaaaagg ctgtcagaat acttaaaact ccaagtgata tttttttgtt
    72361 tatcatgcga atcctcctag taaacgataa cgctttcaca gacattataa aatgctcttt
    72421 ttaaaaattc aagtctttta tgaagtgttt tgaaattgtt agctatctaa atattatgag
    72481 aattctctct tgaattttgg cttaacggcc cctaaaaacg tataggcagt gaactatcta
    72541 ggtttcttcg aaaatccatt agccatttat gatataatag taaagaaaaa catagaaagt
    72601 tggggtctgt tgatgaccaa accatttcat cataagaaac taaaacaaat aactataata
    72661 gctgcaacta gccttttttt attcctgatc ggtggtgcct tttactactc taaaaatcat
    72721 tgtattaatg cctatcttaa agctcgatca gcccaatcag gaccagtttt tgagaatatc
    72781 aaagcatatc tagtctggga tgatactaat gagcagatca caaatgacga ggcgatgtat
    72841 actaagttta gaaggtatag tcaaaaagaa ttgaggcaaa aaaagcagga tttaaaagct
    72901 gctagtcaag atagtgccgt tcaagtaaag tctgttggtc gtcgtttttg gatttttcct
    72961 gattatcgga ttgccataaa acccatggat ttaacgataa aaacgaatgt gcctcaagca
    73021 gacgttcttt taaatcaaaa aaaagttgct gtttctgatt cagaacagtt ctcagtcaag
    73081 cttgatcggc taccaacggc agaatatacc gcaagtatca gaggcaaaca caacgggcga
    73141 aacattaaag tcaataaatc atatgatggt gataatcccg tgctagattt gagtgtgtct
    73201 tttagaactt ttttagtaac aagcaatgct aagcaaggag atctttactt cgatgataac
    73261 catattggca cattaaaaga tggtcaatta caagtagaag attaccctgt tacagaaaac
    73321 gcacaagctt atatgaaaac aacattccca gatggtgagt taagatcaca aaaatatgct
    73381 ctagctgatg ttgaagaagg agcaaccctg gagattttag ttacagatct tttagaagag
    73441 gacaaagcag gggagctatt agtatcagct tttgatcagc tgatgcacta cctcagtaca
    73501 ggtcaagact catctaactt acgtagcgtc tttgaggcag ggtctagcaa cgcattttat
    73561 aggggattga aagagtccat aaaagcgaaa tttcagacag atacaagaaa agccagccgt
    73621 ctcaatattc catctatcct tttgacaaca atgactcaag tgggcaaaac aatttacgtg
    73681 cttgatttca cagctaccta cgaatttttg tatgacaact caacagatcc tgagcagcat
    73741 acctctggac atattaatca agacttgact ggaaaagtga ctgtaaaaaa agttggacag
    73801 cattacctta tcagccagtc cggctccaaa aatattactg ttgttaaaga ggacaatcaa
    73861 ctcaaagcgc catctgtctt tcctgagtct attttgggaa cgtggacagg ccaagccaat
    73921 ggtttgagca tccatatgtc tctagcatca gatggaacaa ttacgactaa agttgaagat
    73981 caaaaaggca accgttctaa agaaactcgg acagctaaaa ttagtaaagt tgaagacaaa
    74041 ggcaatggtt tttatcttta tacgccagat cctggaagtg acataagcgc cttagctcca
    74101 gaaggaggat tggggggtgc aaatgtcaaa tatgcttatg gtttcaaaat atctggtaaa
    74161 acagcctctc cagtggtgtg gcaggcagca ttaacacatg aatttgatta taccaaacca
    74221 ctttcgggag taactttgca aaagcaacca taaaggatgc aaagccacaa agaacagtag
    74281 aaacaatgtt attactcaaa tacagtgcta tgaaaacaga taaactcaaa aggctaggag
    74341 attatttcct agccttttga gcgtttaaga ctgcgtcaaa tgtagctgat ttagcttgca
    74401 cgcaaatgtc ttctcgggtt agagggtgtt tcaaacgtaa ttgataagca tggagcatca
    74461 gtcgagcaca atcttttttt ccattagaat acaaaggatc accaaataaa acatgacctt
    74521 gatgggctaa atggactcga atttgatgag tccgtccagt ttgtaattga caattgagta
    74581 agcttgccgt tttgtggaag tttttgacta gagttacctc agtaatagct ttcttgccat
    74641 ttatcggatc gacaacacgt tttctgcggt catgtcgatg acgaccaatt ggatgatggt
    74701 aagttaccct aggactgtct aaggatccgt gaactagtgc caaatattct cgatggatgt
    74761 ctcttttttc taaaagacga ttaaggattg gtaagataaa aggtgtttta gcaaataaaa
    74821 tggccccact tgtttcctta tccagacgat gaaccacata gcaggtctgt cccgtgtaag
    74881 cagacacgtg gttgagcagc gccaactctg ttggatcatt gccgtgtgtt ttcatccctt
    74941 caggtttgtt aacaataatg atgtgctcat cttcataaag gcaggttact ttttcggcct
    75001 gccccatcac gataatcttt tcaggataat cttcgtgatc gaaaaaaagt ttgacctgat
    75061 caccgtattt aacacaactt tgccagttaa caggatgacc attaataaga acatgttttt
    75121 tagttctcag aaaatgccta attttacggg gaattaacaa ttgttcctct aataatgctt
    75181 tgaccgtcaa ttcttcaaag ggatttttca gtgtgacagt aaaccccttt gatggtttat
    75241 ttgttgtctt cattgagcca gttatggtat ttttcaacaa ggttgatagc catttgggtg
    75301 gcaagagcag cagagaaagc ttcagtacct tcacgctcgt cattagctac ctccaagcga
    75361 gtctcatcgt aaatggcttt tagactttct gcggataagg tttctttaaa agcttggaat
    75421 ttattcattg gttaaacccg tccttctatg tttaatgtga gctcctgatt aatgcaagga
    75481 attcaatgct attatagcat gtttctaatc aaaacgtgcc actcaaaaag gtaaaaacgt
    75541 tattttgcta atccatctaa taagaaagtg tcttaaataa gacctcttga tagagtttgt
    75601 aacaaaaatg atatgaaact aggtgctaac gcagaaatag aaaccaacta acgtgttgcg
    75661 aagttggaac agtcataaaa aatagcaaga taaaacttat caacacacag ttacctaaga
    75721 cgatgagtca aacaggtatg ttctttcaaa acaaccccat gaggttgaac gacgtatcgg
    75781 aaaaatacgg attagtttgg ccttatgata ggtatttctc tatcgtaaaa gaagtcaacc
    75841 aaaggttttt aatataaaat attaagaaaa attgttataa ttatgataaa aacataatcg
    75901 taaaatagag gaaaaacaat gaaaacaatt agacgttatg atgttaacga agatagaggg
    75961 catacaggcc ttgtagaagc aggagacttc tattatttaa actattgtgt cggcaatgtc
    76021 ggacaagaca ttgaaagtca gattaatgga gcctttgacg agatggagcg ccgattagca
    76081 ttggtggggc tcacattaga tgcagttgtt caaatggatt gtctatttcg agacgtttgg
    76141 aacatacctg tgatggaaaa aatgattaaa gagcgcttta atggcagata tcctgctcga
    76201 aaatcgattc aaacggaatt tgcacatcac ggtggaccac aaggactact ctttcaagtg
    76261 gatggtgtgg cttattcaaa acatatttcg atgacctaat caaggttact ttaagatcat
    76321 agtggtataa taacgttatg agatttctag aacttttaca aaagaaattt tttcctaaag
    76381 catatcagga aaaacaattc ttaatgcatc aaaaaacgcg tttaacgcca caacacaatc
    76441 aaaagcagta ttcgccaaat gccaatcatt tggactcatc agctaccaaa aactcagaac
    76501 aagaccctgc aatagctctg caacgcagta gagcctatga aggaagccct aaaagtcggc
    76561 ccgcttggtt gcaaaagctg gaagctgttt tgccgtctcc tcaacatcca attcggcgtt
    76621 tttggcgccg ctatcacatc ggaaaactgc taatgattct gattggaact cttgtcttac
    76681 tcttaggatc atacttgttt tacttatcaa aaacagctaa agtatctgat ttacaagatg
    76741 ccttgaaggc tacaactgtt atttatgatc acaaaggaga gtatgcaggc agtttatctg
    76801 gtcaaaaagg gagttatgtt gagctcaacg ctatttcaga tgatcttgag aatgctgtta
    76861 ttgccactga ggataggact ttttacagta atagcggtat taatcttaaa cgcttcttat
    76921 tggcggtagt tacggcgggc cgctttggag gtggctcaac gattacacag caactggcta
    76981 aaaatgctta tctctcacaa gatcagacaa ttaaacgaaa ggccagagag ttttttttgg
    77041 cgttagagtt gaccaaaaaa tacagtaaaa aagatattct tactatgtac cttaacaact
    77101 cctattttgg aaatggagtt tggggagttg aagatgccag tcaaaaatat tttggaacca
    77161 cagctgctaa cttaacattg gatgaagctg ccacattagc aggtatgctc aaaggacctg
    77221 aaatatataa cccttaccat tctctaaaaa atgctactca ccgtagagat actgttttag
    77281 gagcgatggt tgatgccaaa aagattaccc aaacaaaagc tcagcaagct agagcagtag
    77341 ggctaaaaaa tcgcttagct gatacttatg ttggtaagac agatgactac aaatacccat
    77401 cctactttga tgctgttatt agtgaagcaa tagcaactta tggtctttca gaaaaagaca
    77461 ttgttaataa tggatacaaa gtttacaccg agctagatca aaattaccaa actggcatgc
    77521 agacgacttt taacaacgat gaactatttc ctgtttcagc ttatgacggt agctctgctc
    77581 aagcagctag tgttgcttta gatcctaaaa caggaggtgt tagaggtctg attggtcgtg
    77641 tgaatagtag tgaaaatccg actttcagaa gttttaacta tgcgactcaa gcaaaacgta
    77701 gtcccgcatc aacaatcaaa ccactcgtgg tttacgcgcc agccgttgct tcaggatggt
    77761 caattgaaaa agaactacca aataccgttc aagatttcga tggctatcag ccacataatt
    77821 atggaaatta tgaatcagaa gatgttccta tgtatcaagc attagcaaac tcttataata
    77881 ttccagcagt ttctacattg aacgatatcg gaatcgataa agcctttacc tatggtaaaa
    77941 catttgggtt agatatgagc tctgccaaaa aagagttggg ggtagcttta ggtggcagcg
    78001 tgacaactaa tccattggag atggctcagg catatgctgc ctttgccaat aatggagtaa
    78061 tccatcctgc gcacttgatt aaccggattg aaaatgccag gggtgaagtg cttaaaacct
    78121 ttactgataa ggctaaacgt gttgtcagcc agtctgttgc agataagatg acagccatga
    78181 tgctaggtac cttttcaaat ggaacagcag tcaatgctaa cgtatatggc tatacactag
    78241 ctggtaaaac agggacgaca gaaaccaact tcaatcccga cttagcaggc gatcagtggg
    78301 ttattggtta tacgccagat gttgttatta gtcaatgggt aggatttaat cagaccgatg
    78361 aaaatcatta tctaacggat tcaagtgcag gcacggcttc agctattttt agcactcagg
    78421 catcttacat tttgccttat accaagggca gccaatttca tgtagataat gcttacgctc
    78481 aaaatggtat ttcagctgtt tatggagtca atgaaacagg taatcaatca ggagttgaca
    78541 ctcaatctat tattgatggt ttaagaaaat cagcacaaga agcttcgcaa tcactatcaa
    78601 aagcagtcga tcagtcaggg ttacgtgata aggcccaatc tatttggaaa gagattgttg
    78661 actattttag atagcttgca gtattttaga gatcgtgtta aaataatagt tacggaggcg
    78721 ttatggcaca gaaaaaagcg agtctagcgt gtgtcgaatg tggcagtcgt aactactcta
    78781 tcggagtgag tagcacgcca aaaccaacac gtctagaagt aaataaattt tgcaaatact
    78841 gcaaaacata taccttacac aaagaaacac gttaaaaagg agagcctgat gggattcatt
    78901 agcgggactt ttaaagtctt aaaagacaca acatggccaa atcgtaaaca acgttggaaa
    78961 gattttatct cagttcttga gtacacagct ttctttacgg tgattatcta catctttgac
    79021 cagctcttag caaagagtgt attagcactg attaatttat tctaggtcaa gattggtaga
    79081 aatgataagc ttgcttggaa aagcctaggc tgggagtatt cccagccttt tttgtagtat
    79141 caataatctg attagtaagc ttttttaaag cccatactgg tttgagcttt aaaaaagcat
    79201 aaaggtaaag gcaatgtagc aacgatctcc aaaccaagtg cattaaatta gcttgtaaca
    79261 gagtcaacgc ataaacagcc tggcctcact tgcttagcac attaaaaata gcaatgaata
    79321 ttatggttga ggaaaacagc ctgatagtct gctaaatctt cgggatgata agccttgacc
    79381 tcagccatct catactccct acctagaagt ttgtacttgt tttctccaaa ttgatggaaa
    79441 ggtaacagtt gcacttggtc aatctcaagc tgattaaaga gctctgagaa ggctttagca
    79501 tcgtctaggg aatcattaaa ttggggaata acagggatgc gcaagacgat ttcttttcca
    79561 gcttgaaagg cataatgaat atttttgata atcaagtcat tacgaacccc agtcactttt
    79621 tggtgtctga gctgattata atgtttcaaa tcggtataga taaagtccac gtagtcaata
    79681 agggtgacaa attgctcgtg tttggcaaag gcggtggttt caattgcggt gtgaagacct
    79741 gctgctttgg ctgcttttaa cagggctaag gcaaagtcaa actgagcaaa gatttccccc
    79801 cctgacaggg tcatgccccc acctgactct tcgtagaaat caaggtcttt aaggacttct
    79861 tcgatcacct cgtccacggt tttttcctct ccaactattt tagtattaag cccatcactt
    79921 gttaacatct gctcaggcac tttttgttgg gattcgggat tggcacacca aggacatctc
    79981 aaggggcaac cttttaaaaa aacggttgtc cgaataccag ggccgtcatg aatactaaaa
    80041 tgttggatgt tgaaaacaat accgcgatca gtcataagtg tgaattcctt tcgttgtaaa
    80101 accatcatac cagataaacg taaataaatc aattacgaat gaaagtaaat atcccttttc
    80161 ttttaagaaa ataactggta caatgaaagg gaggtgaatg atgaatcgat tagaacgtat
    80221 tattcaactg gtttcccaga agaaaaaaat agatgttaac agtttatcag agcaattaga
    80281 cgtttcgaaa gtaaccattc gaaaagacct tgataagctt gaaagtaagg gcttgttgcg
    80341 acgggaacat ggctatgcgg tgctaaacag tggcgatgac ctcaatgtac gcttgtccta
    80401 caattacaac ataaagcgac ggattgcaga aaaagcagct gaattggtac aagacaacga
    80461 taccattatg attgaatctg gctcaacctg tgcccttttg gcagaagtgc tttgccagac
    80521 caaacgaaat attaaagtca ttaccaattc gtgcttcatt gccaactaca ttcgtcaata
    80581 tagtagctgt caaatcattt tactgggcgg ttattaccag cccaattcag aggtgactgt
    80641 tggtccttta ttgaaagaaa tgatatccct ttttcatgtc aatcgtgtct ttgttggaac
    80701 agacggtttc aacaaagatc ttggctttat gggcaaggac atgatgcgct cagaaggggt
    80761 gcgttacatg gcagatgctg ctgaggaagt ggtgattctg accgattcca gcaagttttc
    80821 aaaaaccagt ctggtacacc agttgtcatt agctgatgtc aatcgcgtca ttacagacca
    80881 agcactagac aagcaaacac aagagttatt aagtgctagt ggtcttgttc ttgattttgt
    80941 ttcttagaaa ataggtggct agatgaaaga agaacgcaga cggctccttg ccaaggtggc
    81001 ttacttgcat tatgttcaag gcaagagcca aaccttgatt tcaaaggaaa tgaatatcta
    81061 ccgaacaacg gtttgtcgga tgttagcaaa agccaaggaa gaaggcattg ttcggattga
    81121 aattgccgac tatgatgctg atttatttgc tttagaagaa tacgtgcgac agcagtatgg
    81181 cttggaaaaa ctagaccttg tgcccaacca ggttgaagat accccaatgg ataccttgac
    81241 aaatgttgct aaaacagcag ctgaggtttt tagacatgtt gttaaagatg gcgataagat
    81301 tggtctgtcc tggggagcga ctctttcttg cttgatggat gagttaaatc caaaagcgat
    81361 gaaagatgtg tttatttatc ctttggcagg aggacctagc catattaatg ccaaatacca
    81421 tgtcaatacc ctggtttacc gcttggctcg catctttcat ggtaatagcg cttttatgaa
    81481 cgctatggtc attcaagaag acaaacactt ggctaaggga atcttacaat ctaaatactt
    81541 taacgacatc ttaacaagct gggaccagct agatcttgcc ttagtaggaa ttggtgggga
    81601 gcctaatagc cttgagcaga gtcaatggcg tgatttatta accagctcag atcatgacca
    81661 actcaaatat gaaaaagccg ttggtgaagt ttgttgccgt ttctttgatc aggcaggtca
    81721 gccagtttac actggcctac aggatcgaac tattggtatt tcgctagaac agttgaggcg
    81781 tgtccccaaa acaatggctg ttgcaacagg taaacacaag gctaaggcaa tattggcagc
    81841 cttaaaagca ggttttatca attatctagt gacggataaa gagaccatgc tagctgtttt
    81901 agcgttagat gaagatattg atcttaataa cgttcttctt taatagaaat tcaaagatac
    81961 tatgatatgt atagaggccc aagaccaaac gtcagggctt tttttgtttg atgtgttgat
    82021 gaaacagaga ttgctcaaat gtgcagttaa tgtattggca caaaagtgca ttaaccaaaa
    82081 tatagcacaa aatatattga ccctaaaaac aaaaatgata caataaactt accaacgaaa
    82141 tgtaaacgat tacaaacaca ataacatcac cttcgaaaaa aaggctcatt gccgttacat
    82201 cacataaaaa agaaaaaaag gagacaccaa tgcaagttat tgtaccagac caaatcatta
    82261 tgggcttaat tttaaatgca ggcgatgcca aacaacatat ctaccaagct ctgaaatgtg
    82321 ctaaagagga cgattatgcc actagtgaaa aagagatggc cttagcagat gatgccttat
    82381 tagaagccca caatttacag acccagtttc ttgcccaaga agctagtggt aataagtcag
    82441 agattacagc tctttttgtt cactcacagg atcacttgat gacgaccatt acagaaatta
    82501 atctcgtcaa ggagattatt gaccttcgta aagaattggc aaccaaataa tatttcattc
    82561 ataaaaagga gattgacatg attaaaattg gattgttttg tgcagcagga ttttcaaccg
    82621 gtatgttggt gaacaacatg aaagtagctg ctgaaaaaaa aggcattgat tgtcagattg
    82681 aagcctatgc tcaagggaaa ttagcggact atgcaccatt acttgatgtg gcacttttag
    82741 gcccacaggt tgcctatact ctggatcaat cagaagctat ttgtaaagac aatgatatcc
    82801 ccattgcagt tatcccaatg gctgattatg gaatgttaga cggcaacaag gtgcttgatt
    82861 tagcccttag tcttgttaaa gaataggagt caatcatggc aaaaatgaat atgcaaaaca
    82921 tcatcatgcc aatcatgaaa tttgtgaata tgcgcggtat catcgcctta aaagacggga
    82981 tgttagctat tttacctttg accgttgtgg gaagtctttt cctgattgct ggtcagattc
    83041 cttttcaagg gattaatgat gccattgccg gtgtgtttgg tgccgactgg acagaaccct
    83101 ttatgcaggt ttaccatgga acgtttgcta ttatgggctt gatttcctgc tttgcgattg
    83161 gttattctta tgctaaaaat tcaggtgttg aacctctccc ttcaggggtt ctatccttat
    83221 cagctttctt tattctattg agatcatctt atgttccagc agaaggcgaa cctgtcggtg
    83281 atgctattag caaggcttgg tttggtgggc aagggattat tggtgctatt gtgattggtt
    83341 taacggtagg tgctatttat acagcattta tccgtcgcca cattgtcatc aaaatgccag
    83401 agcaagtgcc acaagccatt gccaaacagt ttgaagctat gattccagcc tttgtgattt
    83461 ttaccttgtc aatgcttgtg tacattattg ccaagtcagt gacaggtggt ggcaccttta
    83521 ttgaaatgat ttatgatgtc attcaggtac cgctgcaagg gttaacgggt tctctttatg
    83581 gcgcactagg tattgccttc tttatttcct ttctttggtg gtttggcgtg catgggcaat
    83641 ctgtggtcaa tggaattgtc actgctcttc tcttatcaaa tctagatgcc aataaggcct
    83701 tgatggcagc aggtgagtta tccctagaca agggtgccca tattgtaacc caacaatttt
    83761 tagactcttt cttgattcta tcaggttctg gcattacctt tggtttagtg gtagctatga
    83821 tctttgcggc taaatccaaa caatacaagg ctttaggtaa ggttgcagcc ttcccagccc
    83881 tctttaatgt taatgaacca gttgtctttg gttttccaat tgtgatgaat ccagtcatgt
    83941 tcttgccatt tatcttggtt cctgtcttgg cagctctcac agtttatggc gccattgcta
    84001 ttggtttcat gcagcccttt gcaggagtga cccttccgtg gtcaacacca gccattatct
    84061 cagggttcat ggttggtggc tggcaaggag ctattgtgca aattcttata ctcatcatgt
    84121 caacgttggt gtacttccca ttctttaaaa tccaagataa tatggcttat caaaatgaac
    84181 aagctagtga agagtcatga tttagcgtcc acacaaattc ttgagaccat ctccagttag
    84241 aggtggtttt ttagaaaaag aagagcgact tatcaggcct atattgcaca atagtgcaaa
    84301 ataaagtgac tatctcttct tatggttaag gatacgcaaa agtcgcaata tatgataaaa
    84361 tagttacgaa agaaacattt ataatttcgt aagaaagaag gagaccccgc atgactgaaa
    84421 caaaaagccc ttattttgga catttaacag acagaatgac tcactatcgt gaagctgtgt
    84481 tagacaagaa accttatatt gatgctgagc gtgctatttt agccactgaa gcctatcaaa
    84541 aacaccaaaa taagccagca aacctcaaac gcgcttacat gcttcaaact attttagaaa
    84601 acatgaccat ctatattgag gatgaaagcc tcattgctgg taaccaagcc tcttcgaaca
    84661 aggatgcgcc gattttccca gaatacactc tggaatttgt cctcaacgaa cttgatcttt
    84721 ttgaaaaacg agatggggat gtcttctaca ttaccgaaga aaccaagcaa cagctccgag
    84781 acattgcccc attctgggaa aacaataacc tgcgtgctcg ctgcggtgtc ttgctaccag
    84841 aagaagtgca agtgtacatg gaaacaggtt tctttggcat ggaaggcaag atgaactctg
    84901 gggatgccca tttagcggtt aattaccaaa aactcttgga acacggctta aagggatttg
    84961 aagaacgggc acgagctgcc aaggctgcgc ttgatctgac cattcctgaa aacattgaca
    85021 aataccattt ttacgactct gtttttattg tcattgatgc agtcaaaact tatgctaaac
    85081 gctatgccaa gttggctaga gaactagcaa aaacggctaa accagaacgt caagcagaat
    85141 tgttagacat cgcacgcatt tgtgacaaag tgccttacga accagcgaaa acctttgcag
    85201 aggctgtaca atccgtctgg ttcattcagt gtatcttgca aattgaatca aacgggcatt
    85261 ccctatctta tggccgcttt gaccagtaca tgtatcctta tgtcaaagct gatttggagg
    85321 ctggccgtga aacagaagcc tcaatcgttg aacgtttgac caacctctgg attaagacct
    85381 tgaccattaa taaagtacgg agccaagccc acaccttctc atcagcaggt agtccacttt
    85441 accaaaacgt gacgatcggc ggtcaaacac gcgataaaaa agacgcggtc aacccactgt
    85501 cttaccttgt cctacggagt gtggctcaaa ccaaattgcc acaacctaac ttgactgttc
    85561 gttaccataa aggcctagac aataccttta tgaacgaatg tattgaggtc atgaaacttg
    85621 gttttggcat gccagccatg aacaacgatg aaatcatcat tccatccttt atcaaaaaag
    85681 gtgtctcaga agaagacgct tatgattatt cagccattgg ttgtgtggaa acagcagttc
    85741 ctgggaaatg gggctaccgt tgcacgggga tgagttacat caacttccct aaaatcttgc
    85801 tgattaccat gaatgacggg attgacccag catcaggcaa gcgctttgca aaaggacacg
    85861 gtcatttcaa agacatgacc tcttatgaag aattaaaagc agcatgggat gccaccctgc
    85921 gcgaaattac ccgcatgagc gtcattgttg aaaatgccat tgacctaggt cttgaacgtg
    85981 aagtaccaga tattctttgc tcagccttga cagatgattg tatcggtcgt ggcaagacct
    86041 taaaagaagg cggagctgtt tacgactata tctcaggcct tcaagttggt attgccaacc
    86101 tatcagactc cctagcagcg ctgaaaaaat tagtgtttga agaaggtcaa ttaactccag
    86161 aagaactctg gcaggccctt gaaagtgact ttgctggaga gcgcggagaa gagatccgac
    86221 aaatgctcat caatgacgca ccaaaatatg gtaatgatga tgactacgca gatagtttag
    86281 ttgtcgaagc ttacgacacc tacattgatg aaattgccaa atacccaaat acccggtatg
    86341 gccgtggccc aatcggaggt atccgctatt ctgggacatc atcaatctca gccaatgtcg
    86401 gacaaggaaa agggaccttg gcaacaccag acggtcgcca tgcagggaca ccactagcag
    86461 aaggctgttc accagaacac agcatggaca aaaaaggccc gacttcagta ttgaaatctg
    86521 ttgctaaatt accaacagat gagattgtcg gaggagtgct actcaaccaa aaagttaacc
    86581 cacaaaccct ggccaaagaa gaagacaaac tgaaattaat ggccttactt cgtactttct
    86641 ttaatcgcct acacggttat catatccagt acaatgtcgt gtcacgtgaa accttgattg
    86701 atgcccaaaa acatcctgaa aagcaccgtg acctcattgt ccgtgttgcc ggctactcag
    86761 ctttcttcaa tgtgctctca aaagcaaccc aagatgacat tattgaacgt acagaacaca
    86821 cactttagga gaaaataagt atggaataca tgttagacac tttagattta gaagcgatta
    86881 aaaaatggca tcacattttg cctctcgcag gtgtgacctc aaacccttct attgccaaaa
    86941 aagaaggtga cattgatttc tttgaacgga ttcgagaagt ccgtgctatt atcggtgaca
    87001 aggcttctat tcatgttcaa gtgatcgccc aagattacaa aggcatctta aaagatgctg
    87061 ctgaaatccg caggcaatgt ggagatagtg tgtatgtcaa agtacctgta accacagagg
    87121 gccttgcagc cattaaaacc ttgaaagctg aaggctatca tattaccgcg acagctattt
    87181 acagcacttt ccaagggtta ttagccattg aagctggcgc tgattatttg gctccttatt
    87241 ataaccgtat ggaaaatctt aatattgatc cagaggccgt gattgggcaa ttggcagaag
    87301 ctattgaccg tgagtgttct gacagtaaac tcttagcagc gagctttaaa aatgtggctc
    87361 aggtcaataa atcctttgct ctaggagcac aagccatcac tgctggccca gatgtgtttg
    87421 aagcaggatt tgccatgcca tctattcaaa aagcggttga cgattttggc aaggattggg
    87481 aagccattca tcaccgcaag agcatttaag aaatagcatc agtagtaatc acatcattag
    87541 gaatcacaga taaaggagtt caacgatgaa agtatttgca agtccatctc gttacattca
    87601 aggtaaaaat gccttgttta ccaatgctaa aaccctaaaa caactaggaa atagccccat
    87661 tttactgtgt gatgacgtgg tgtatggcat cgtcggagaa agatttgaaa gctatttgat
    87721 agataatggc atgactcctg ttcacgtagc ctttaatggc gaagcctcag ataatgaaat
    87781 cagccgcgtg gttgccattg ccaaggaaaa tggcaatgat gttattattg gacttggtgg
    87841 tggaaaaacc attgacagtg ctaaggctat cgctgactta cttgctgttc cggtgattat
    87901 tgccccaacc attgcttcaa cagatgcccc aacctcagcc ttatcagtta tttacaccga
    87961 tgaaggagcc tttgaaaaat acattttcta ttcaaaaaat ccagatcttg ttttggttga
    88021 cacacaagtg atctgtcagg caccaaaacg cttattggcg tctgggattg cagatgggtt
    88081 agcaacatgg gtagaggcgc gtgctgttat gcaaaaaaat ggagacacca tggcaggtgg
    88141 caatcaaacc ttggcaggag ttgccattgc caaagcctgt gagcagacct tgtttgcaga
    88201 tggcctaaaa gcgatggcta gctgtgacag acaagtcgtg accccggcct tggaaaatgt
    88261 cattgaagcc aatacacttc ttagtgggct tggttttgaa agtgcgggtc tggctgctgc
    88321 acatgccatt cacaatggct ttacagcgct gacaggtgct attcaccacc ttacccatgg
    88381 tgaaaaagtc gcctatggta cgttgaccca actcttcttg gaaaatcgtt cgcgtgaaga
    88441 aattgaccgt tacatcgact tttaccaagc cattggcatg ccaacaaccc tcaaagaaat
    88501 gcatttagac acggctactc aagaggattt cttaaaaatt ggccgtcaag caaccatggc
    88561 aggcgaaacc atccatcaaa tgccgtttgt tatcagtcca gaagatgtag cagcggcttt
    88621 ggttgctgtt gatgcctatg ttacgagtcg ataggctaac caaaaagaga tttcttattt
    88681 gccagataac ctcaaaaaaa caagtctagg gagtcacttc ctagacttgt tttgcttatt
    88741 gttggttggt aatagcactt ttagcggggt cattgtgggc aatacgactg gcagctgcag
    88801 cagcaatggc gctaacaata tcatctaaaa aggtatggca ggagttgcca tctttatggt
    88861 tgagtttgtc aacaattcct ggttttgtct tgtccagata cccataattg gtaaaaccga
    88921 tagacccata gaggttaaca atggataagg ccaggatttc atcaatccca tacagccctt
    88981 ggtcggtttt caagatacta aggagtggct cagacagctg gttggcttca gccaatttat
    89041 ctaattctac cccagtgata atagcgtttt gcacttctcg ttttgcaagc accgcctcaa
    89101 cgctctctaa acattctgcc atggtcaaat tgggaatata atcattctgc aaaaaaagca
    89161 ccagctctgc aatagcttct aaagaacccc ctcgctcagc cagtaattga taagacactt
    89221 ctcgtaggtg atggtcagtt gacatgtgag acctcctttt tttctttatt gtaacataaa
    89281 agatttcgag aaacctctac aacagctaaa gaggggatag ttctttttta agagatgttg
    89341 actactgaga tcccctactt ggtaaaagtc aaagaaacga tagtaaagta accatcttac
    89401 ctaggcctaa atacaatcac cacccctagg tgacacacag cacctatttc aaaaatgata
    89461 ttagtatggc aaaacaaaaa gacaacgcct tcttttttct ccttactatc tcctttaatt
    89521 ttcatatttt ttaaaaaaac tattgataaa ctagttaagt aagcgtatac tatggttagt
    89581 gagcgaaatt agaaaagagg acaagcatat gaatctactt ggatcaagac gggttttttc
    89641 taaaaaatgt cggttagtga aattttcaat ggtagctctt gtatcagcca caatggccgt
    89701 aacaacagtc acacttgaaa atactgcact ggcacgacaa acacaggtct caaatgatgt
    89761 tgttctaaat gatggcgcaa gcaagtacct aaacgaagca ttagcttgga cattcaatga
    89821 cagtcccaac tattacaaaa ccttaggtac tagtcagatt actccagcac tctttcctaa
    89881 agcaggagat attctctata gcaaattaga tgagttagga aggacgcgta ctgctagagg
    89941 tacattgact tatgccaatg ttgaaggtag ctacggtgtt agacaatctt tcggtaaaaa
    90001 tcaaaacccc gcaggatgga ctggaaaccc taatcatgtc aaatataaaa ttgaatggtt
    90061 aaatggtctg tcttatgtcg gagatttctg gaatagaagt catctcattg cagatagtct
    90121 cggtggagat gcacttagag tcaatgccgt tacagggaca cgtacccaaa atgtaggagg
    90181 tcgtgaccaa aaaggcggca tgcgctatac cgaacaaaga gctcaagaat ggttagaagc
    90241 aaatcgtgat ggctatcttt attatgaagc cgctccaatc tacaacgcag acgagttgat
    90301 tccaagagct gtcgtggtat caatgcaatc ttctgataat accatcaacg agaaagtatt
    90361 agtttacaac acagctaatg gctacaccat taattaccat aacggtacac ctactcaaaa
    90421 ataataccaa aaggctagac ctctgctcac taggcctagc tttttacatc aaaaaaagca
    90481 atgactatag aaagtaaaaa tactagaaaa agcaatgatt gccgtcattg ctttttatga
    90541 tttgtcaaaa agtaaaaagc aaattgaaaa aattgccctg gagctattga gataaactac
    90601 accatttatt aagataccaa atcaaaaagc taacaccata agagcgttag ctttttaaag
    90661 agcctcagga cagtttatgt ttaatggctt ctaggtaggt ttgaaacata tgatggatcg
    90721 ttttgcaatt aagtaattgg aagatctcca agatctgcac acacttttgc tcagccccct
    90781 ctttaccatt tatgaggcca ataatgccat caaaaaaaga cacacaacac cgttcatata
    90841 agcaatcatc atttagctga taatcttcaa tttttgccaa aatctcagaa gctttatcat
    90901 actcttgtcg ctgaataaac aaaatcaaca tattgacaaa catccgaatc gattcattcc
    90961 cataatacct tagggtattg tatttatcga ggttcaaaat aacttttgaa aacaccatct
    91021 caataaagca agactcaaaa ataaacatac aattattaaa aagcacagtc tcatagtgac
    91081 tccaagtttc aatattaata agatagtttg acaaataagt ccgctctgtc agacagtctt
    91141 cctcagtaag agttgctaat aacaccttga ccaaggcaaa aaggttcttt tcctttgttg
    91201 atttactatc tttataagaa tcaaggatat gatttaaacc atctatgttt cggcattcaa
    91261 aataatgttt tgccgtatcc atatcaataa attctttata ttgtttaaag ttattactaa
    91321 taaagagaaa ctcatcaaca ttgacattca agttgtctaa gatataaagt aactttttcg
    91381 aagaaatgtc aagattattt ttaataaaac gataataggt ttgcctagtg agataatcac
    91441 cacaaacttg tttaatcgaa atgttttttg aatgcctaat gaattcaacg gtttcaccaa
    91501 tttccatatg tcaagccttc ctagttgatg tcaaaaatac gttacgcatg tacctaatac
    91561 gtaacaagtt gaatgtttcg gatgatagtc gcttatgata ggtgcataag gtcaatagcc
    91621 agatgcgata gttttatcaa ctgtcatatg ttaaaccttt ctaatctaat agatgttaaa
    91681 aatacgttac gtgtgtacct aatacgtaac aaaataatgg gttagcaaaa taagcagcta
    91741 tgatatagcc ataaggttaa aaggaggcgc ctactatgtg gttattgtta ctatttttgt
    91801 agttcctttt gcaaaatggt agtagataaa aatgagaagt caataaaagc attgacaaac
    91861 aaaagaatca ttttgtttat attatcagta tttttaagtt tcttgtcaac tgaaatgagc
    91921 atctactagc cacaatagta actaaaaggc taattgtcag taaaaatgag ataaatcagc
    91981 caggaaaaag cctacgagtc aactaatgga caacccaaaa aaagacaaag gattttcact
    92041 tggtgttgtg gaccctatca cgatccttat cacagaatca gttattaaca aaatgagaac
    92101 ggtcccatat catctaaaaa aactgtcaag cacaacctat tgtttgctag ctgatcttgc
    92161 ctatcagtct gcctagcaga tacggcagaa gataagagat caaccaaaag atgataagtg
    92221 caacgactca tagcgtcttt tgaccaaaac caattaggct cacatgagat gagaataatt
    92281 gggttgggtt gtcagtgtca actaaccgtg ttattgtcta ttaccattca tggtatcagc
    92341 gacatcgtat gataaccata cgattcagct aagtaaggag gtgtgtccaa tgtaccgtta
    92401 aaagcaaatg cagtagatta acttattttg aaagaggtat aaaaaaaatg aataaaaaga
    92461 aattaggtat cagattatta agtcttttag cattaggtgg atttgttctt gctaacccag
    92521 tatttgccga tcaaaacttt gctcgtaacg aaaaagaagc aaaagatagc gctatcacat
    92581 ttatccaaaa atcagcagct atcaaagcag gtgcacgaag cgcagaagat attaagcttg
    92641 acaaagttaa cttaggtgga gaactttctg gctctaatat gtatgtttac aatatttcta
    92701 ctggaggatt tgttatcgtt tcaggagata aacgttctcc agaaattcta ggatactcta
    92761 ccagcggatc atttgacgct aacggtaaag aaaacattgc ttccttcatg gaaagttatg
    92821 tcgaacaaat caaagaaaac aaaaaattag acactactta tgctggtacc gctgagatta
    92881 aacaaccagt tgttaaatct ctccttgatt caaaaggcat tcattacaac caaggtaacc
    92941 cttacaacct attgacacct gttattgaaa aagtaaaacc aggtgaacaa tcttttgtag
    93001 gtcaacatgc agctacagga tgtgttgcta ctgcaactgc tcaaattatg aaatatcata
    93061 attaccctaa caaagggttg aaagactaca cttacacact aagctcaaat aacccatatt
    93121 tcaaccatcc taagaacttg tttgcagcta tctctactag acaatacaac tggaacaaca
    93181 tcctacctac ttatagcgga agagaatcta acgttcaaaa aatggcgatt tcagaattga
    93241 tggctgatgt tggtatttca gtagacatgg attatggtcc atctagtggt tctgcaggta
    93301 gctctcgtgt tcaaagagcc ttgaaagaaa actttggcta caaccaatct gttcaccaaa
    93361 ttaaccgtag cgactttagc aaacaagatt gggaagcaca aattgacaaa gaattatctc
    93421 aaaaccaacc agtatactac caaggtgtcg gtaaagtagg cggacatgcc tttgttatcg
    93481 atggtgctga cggacgtaac ttctaccatg ttaactgggg ttggggtgga gtctctgacg
    93541 gcttcttccg tcttgacgca ctaaaccctt cagctcttgg tactggtggc ggcgcaggcg
    93601 gcttcaacgg ttaccaaagt gctgttgtag gcatcaaacc ttagtatgga aatgcatttc
    93661 gttagaacag aacctgaggc acgccgcata gctgaaacct tttgtgccga aaacacacaa
    93721 acaaaaaccc ctatgcgcgt gcagcagtta agctatccat cagacacaga tcactcaggt
    93781 ggtgagctat acatctatgc tttgtctcct gctggattta tcatcgtatc aggagacacc
    93841 agagcgcaca ccattttagg ctattctttt gataataacc tggacctcaa ccatgataat
    93901 gtcagaagta tggtagaagc ttaccaaaaa caaatcaact ctttggattg atcggtcaaa
    93961 gtagcttttg ccccctcacc actcgtttaa gacgaccgtg tttctaaaat accactacct
    94021 tttaagtttc acgccggcct aagcaagtgt ttgtctcatg gagcagactg tttccatcga
    94081 aaagtcaccc attgataaag gaggtcatca acgcctacta atgatgactt ccctaagatg
    94141 ccaaggtaaa ggactgagtt ctgtagagat gggactcagt ccttttacga tttttgggag
    94201 ttattctagc cataccgtgg caagaaaaca acactcaaaa atagccaacg tcagtttctg
    94261 ttcatacatg actacagcca aacaccacaa cctaacctat cctaaacaca gtggctttct
    94321 aacgaacagt cacaacgagc agcaacacca aaaagcaaga ttaagctacg tttaagacta
    94381 atgatataag ataacagtaa ttattaatga gaaaagaggc tcaaaaggac atgaaacaaa
    94441 tgaataaact cattacagga gtggtaacgc tggcgacagt cgtgacctta tcagcttgtc
    94501 aatcatcaca caacaacacc aaactcgtct cgatgaaagg agacaccatc actgtcagtg
    94561 acttctacaa tgagaccaaa aacacagaac tcgcacaaaa agccatgtta agcttggtga
    94621 ttagccgtgt atttgagaca caatatgcca acaaagtctc tgacaaagag gttgaaaaag
    94681 cctataaaca aaccgcagac caatacggta cctcctttaa gacagtccta gcacaatcag
    94741 gcttaacgcc agaaacctat aaaaaacaaa ttcgcctcac aaaattagtc gaatatgccg
    94801 tcaaagaaca agccaaaaac gaaaccatct caaaaaaaga ctaccgtcag gcctatgacg
    94861 cttatacccc aaccatgacc acagaaatca tgcagtttga aaaagaagag gatgccaaag
    94921 cagcccttga agccgtcaaa gctgaagggg cagactttgc agctattgcc aaagaaaaaa
    94981 ccattgcagc cgataaaaaa ataacctaca cgtttgactc aggcgaaaca accctaccag
    95041 cagaagtagt tagagctgca tcaggcctca aagaagggaa cagatcagaa atcatcacag
    95101 cgcttgatcc agccacctca aaacgcacct accatatcat caaagtcacc aaaaaagcaa
    95161 ctaaaaaagc agactggaaa gcgtaccaaa aacgcttgaa agacatcatt gtgactggca
    95221 aattaaaaga ccctgacttc caaaacaaag tcatcgctaa agctcttgat aaagcaaatg
    95281 tcaaaatcaa agacaaagca tttgccaata tcttagccca gtttgcaaaa cctaaccaaa
    95341 aacaacctgc ccaaaaatag aaggttgcca gtttatttcc ctaaggttgc ttaaaaagac
    95401 agccctagcc atcactcccc ttttcaaaac accgataatg gaaacgtgtc ctatactgat
    95461 ggtctagggc aatgtcataa agctaaagac tctatccaaa taaggataga gtctttaatg
    95521 ttttaaaacc agtcagcaaa aaaattcacg ctaagcctag aatgaggctt agcgtgaatg
    95581 gtatcagtga tagtccgtta aactagctga ttgggagtca gaaactctcc ttacccaatc
    95641 tgactatacc cccagtacca gaggcgtctc atcgcagaag tagtaatcag ataatcgtct
    95701 gataagctct tgtcctcaag caactgatga gtatcgtcgc acgggccatg agctgtcggt
    95761 ggctgatagc tagtaggctc atcagccaaa acttttttgg agtaacgctt tggtctcaga
    95821 cggcgccaga gggttgagag aggggtgaga aaaaaacgat gggtcatctt ggcctccttg
    95881 tctaatgata gtcaccttaa cagacaaaag tgtcaataat gttattttaa ggagaaaaag
    95941 attaaaaatc aaggtcacaa actttccttt gttaaaaaaa cgcgacccta ataaagactc
    96001 taaatacccc tcgcatttga gcttctcctt gcgcagctat ctgcgcactc agttgcctcc
    96061 aagctaatga tagcgatacc attattgtcc tataggctat cgcggcaacc ccttttttcg
    96121 tctcaacgag tctgacacag ctgtcatgac agagaaaaga ctcttgtcta tctaagagat
    96181 cattaatttt tctctcacca aaaaaatgat tttgggaaca aaaaagtatg gtgtataata
    96241 agtttcggaa caattatgac ataataatga aagtaaggtt aacgaaacat tgacaaattg
    96301 taagtataaa cttagaaagt tatctgtagg gctcgtctcc gttggaacga tgctgatagc
    96361 cccgacagtt ttaggacagg aggttagtaa tactgaggct agtgctagta gtaccgctag
    96421 cgtagacgct acggcaagtg ggacgactgc cagcggagct agtagtgaag caaccgtagc
    96481 tactaccaat ggaggaagtc agtctactca agtagcagct gaaacgactc cacaacctca
    96541 agtacagact tcagaacaac cagcagctac atccacatca ttggcttctt ctactagtag
    96601 tagtgaagat aaagctccta aggcagcaag cactaaatca tcttcagcaa ctgtggctag
    96661 ctctagtaat ggtagcaatc aaggtgctgg tactgaagtt gaaccacaga tgatggacgt
    96721 ggaacagtat aaggttaata aggaaaaaac agagcttact gttaaagatg acaaccagca
    96781 gctcaagatc cgtaaagatg ttgatgaatt aaaaaataaa gatctttttg atgtcaaacg
    96841 tgaagtgaaa gataatggcg acggaacctt agatgtaacc ttaaaagtaa cacctaaaca
    96901 aattgacgaa ggtgccgatg ttatggccct tttagacgtc tctcaaaaga tgacagatgc
    96961 tgattttaac aacgctaagg aaaaaatcaa gaaattagtc aaaaccttaa cgagtaaacc
    97021 tactgatagt catccaaacc ataatgctcg caactctgtg cgcctaatga ccttttaccg
    97081 tgaggttagt gacccaattg atatatcagg aaagacggat gaggaactca ataagatatt
    97141 agacgatgtg tggcaaaatg ctaaacataa ttgggactgg ggggttgact tgcaaggagc
    97201 tatccataag gctcgaggga tttttaagca agaccaagaa aaaaaatcgg gtaaacgcca
    97261 gcatatcgtc ttgttctctc aaggtgagtc gacttttagt tatgatattc aaaagagtga
    97321 gaaagagaga gacaacaatt tatctcgtat aaatgaaaaa attacctctt ctaacccttt
    97381 gttacattgg ccacctattt ttaaccacac gcatcaaaat gcagatatgt taaatgaaat
    97441 cgaaaggatt ataaagatag gggagcaact aggtataaaa ggcctttcta gtataaaagg
    97501 gtatttaagt acggcagcag caggtagcgg gattctggga aattttatcg gtggaggaag
    97561 tcttacagaa tacttaactc ttaaagaata taactctgat aaattagaag aaagccaatt
    97621 tgattacacc aaacgtgtgg gcgagggata ctattattat agcttctcaa aaagagtaag
    97681 tcacaacgat ttccctttta aaactttaat agagcaggct atcacacaag cgattcctac
    97741 taagggtgaa agtaaaaatt ggttcgagaa atttttagat aaattttcat tggtggaaag
    97801 ttatcgagac actcaaaaaa aagcattatt aaagattctt gaatatcttt tttataagcg
    97861 tgaataccac tactataatc acaatctttc agcgatagct gaagccaaaa tggctcaaca
    97921 agaaggcatc accttctact ccgttgatgt tactgattca gaaacaactc ctaaaagagt
    97981 gaggcgacaa acagcagtac gtcaggaaaa agcgaaaaaa gaatccaaag aagagcgcaa
    98041 tgaaaagttt gataaatatc tgaaaaagat gtctgaaggt aatactttct tagatagtgt
    98101 tgaagagaaa gataagttca aagacacctt caccaatgta acagtgacag aaaccttcga
    98161 agataaggtt tccgctatag caaattcttg ggaatcttcg ctagatagtc ataataacaa
    98221 atcagacaga aatcataaag gaagtgtcaa acatacacaa gctcaaagca gcggatggtt
    98281 tgccaatacc caagaaagcc tcacttggac tatttccaaa gaccagctca aaaaagcctt
    98341 tgaagatgga gaaccgctaa ccctcaccta taagcttaaa gttgataaag ataaatttag
    98401 agaaactctt aaaaaacaac aagaacgtaa tagaaaaaaa cgagcagcat ctccggaaag
    98461 tgagaacact gtcacagaaa aaattatttc aaataagatt tcttacaaga ttaataatgg
    98521 taaggatata aataacaata agttggaaga tgttaaaatg tcttacagca agttcaaaat
    98581 gcctatacca gaacttgata tagaagttgt accaatacca gaaaaaccac tggtagaacc
    98641 aatgacgcct ctatatcctg caattcctaa ttaccctacc cctcaacttc caaaagatga
    98701 agatctggag attagtggag gtcatggacc gattgtcgat atcgtcgaag atactggtac
    98761 aggtgttgag ggcggctctc aaaacggcgt ggtttcaact caggaaaata aagatccaat
    98821 cgttgacatc accgaagata cccaaccagg catgtcaggc tcaaatgacg cgacagttgt
    98881 cgaggaagac acagcaccta aacgtccaga tatccttgtt ggcggtcaaa gtgatccaat
    98941 cgatatcacc gaagataccc aaccaggcat gtcaggctca aatgacgcta ctgttatcga
    99001 agaagatacg aaaccaaaac gcttcttcca ctttgataac gagccacaag caccagaaaa
    99061 acccaaagag caaccaagta acagcttacc acaagctcca gtctataagg cagctcatca
    99121 cttgcctgca tctggagaca aacgtgaagc atcctttaca attgttgctc taacaattat
    99181 tggagctgca ggtttgctca gcaaaaaacg tcgcgacacc gaagaaaact aaccctcgtt
    99241 agtctagcga ccctaagcct ttggctttaa aaatttctca ttatccccct caaacaccgg
    99301 tacctcactg agatggcgct taaaaaagcc gctagtctta aatcaggtat cccctacggt
    99361 tgagggggat taatgggtat cacaacaagt aaaaaggtat aatgatgaaa tatgactaaa
    99421 aaatttatta aatcagcagt gattctaggc ttagcaagca taaccatcag catgacgcaa
    99481 cccgttgaag ccatttcaaa tagtttagaa tatcaagcac aatataaacg tgtcaaagaa
    99541 gaactagacg cattaaaaga aaagggccca aaattagggg aacgttccct ttattttaaa
    99601 gtggtgaata gatttctagg ttggtttggc gtagtctggg acccactcgc aatcaaagaa
    99661 accgatcacc tgttggacct catcggacaa gtcaattact tggtgagtaa caccgagttt
    99721 caagcccctg agtatcaaaa ggctaaaaac aaactagact tagctgtagc aggctatgac
    99781 ctctacctag ctagagagaa aaaacgtggt gatgctcctg attaccaagt gacccagcta
    99841 acgctaggac agctcgaaac catcaaaacg aagatacaag aggccctgaa aacatttgca
    99901 caagaggtca aaacgatcca gcaaaaaaaa ccagacctat ttccccatgc ttatgtttta
    99961 aacgctaaga gcaaagatat cacagataaa gcgcaaccct ttttagtgaa tggttactac
   100021 gatgtctttt ataagcagtt tttattcact aatacaaatg acggctacaa ctataataaa
   100081 gaagcagaca atctggcttc gtatcaaaag ctgattaaag agctattagg cttaaaagct
   100141 gagatcgatc agttaacaaa agacgatcct aaaaacgatc gtttagagac gttaatgtgg
   100201 gaatcaaagt tcaccttatc tgattcacgg agaacttcac gccttagcgc ctactataca
   100261 caagaagatt atgtcaaaaa ggtggtccta tctgaagaag ctcccaactt gagaacagca
   100321 aaagaaaagg tcaagacctt aaaagggatc ttgcatgatt actatgttga cattaaagag
   100381 ccagaaaaag ctaaaaagta tcgtgtcgag gacgagacct tgccgtctca acctgaggcc
   100441 cctgctaaac ctgaagctcc tgctcctagt cctagtcctg ctcctggtca aaaaccagca
   100501 gaagatgaga gatcatcaca agctacggag ccagctaacc cgtcaaaaga gaattcctca
   100561 acggacgcat ctcagggatc tcacgatagt gaaaacccag cgacagacag tccttctcaa
   100621 cctcaggcat cagaccaagg caatcaccaa agccaagtgc ctaacgccaa gcctcaaacg
   100681 gacaagacgg acactcctaa cgccccagtc cctccacaga acactcctaa cgccccagcc
   100741 atcccacagg acaccccaaa agttcctgag gctggtggtc agagtggccc cgctgggaac
   100801 gctgaggaga aagctcctga ctctggacct aaagagtctg gtcaaagctc atctaaagag
   100861 tccccatccg ttggcgaaga cacgacccct aatcagccag acgtccttgt aggtggtcaa
   100921 cgtgagccaa ttgatatcac cgaagacaca atcactgacg ctcctccgac agtatctggg
   100981 cacaacgcat caacacagcc ccaatctgtg gtcgaagata cagcacctca gcgcccagat
   101041 gtccttgtag gtggtcaaag cgagccaatc gatatcaccc aagataccca accgggcatg
   101101 tcaggctcta atgacgctac cgttatcaat gaagatacaa aaccaaaacg cgtcttccac
   101161 tttgataaca aagagccaca agcatcagaa aaagcggcag agcaaaaact tgctcctcat
   101221 gatagccaca caacccctca aacttcagat gatacagcag cccctcactt gcctgcatct
   101281 ggagacaagc ctgaaaaagt gtttgcactt gctgccttga caatggtcgc agctgcaggg
   101341 ctactcagca aaaaacgtcg caacaccgac aaaaactagt cgctgttaga cacaacgcat
   101401 cactaaggcc agtgcatttg cactggcctt agttaggttt ttatcaaata gagggttttt
   101461 catgacaatt gctagcattt cggtgtaaaa taaaggtaat aatcagcaaa ggagcaaccc
   101521 atgacaaagc aagatcagtt aatcgttgaa aaaatggaac aaacctatga ggcctttagc
   101581 ccaaagctag caaatctcat agaagcccta gatgccttta aagagcatta tgaggaatat
   101641 gccacactta gaaattttta cagtagtgac gaatggtttc ggcttgcaaa ccaaccttgg
   101701 gatgatatcc cgtgcggtgt cctatctgaa gacctcctct ttgatatgat cggtgaccat
   101761 aaccaactac tagctgatat cttagacttg gcccctatca tgtacaagca tatgtaatca
   101821 tcgctaacgt cacagatact acgatgatag gacaaaaaaa gcttgaaaat ctcaaaaaaa
   101881 acagataaaa aacagataat cgttagaaaa acagtagatg cgtctgttaa actaaaggta
   101941 tcaaaacaaa aggagcattt catcatgaaa ttcaaaaaag tattagtcat tcctgcatta
   102001 gcattagcag caacatgctt tttaacagct tgtggcacaa agaaagattc taaaaaagaa
   102061 gaagttaaag aaatcaaaat gtctgacatc aaagacgacg cagttagcaa aaaaacaaaa
   102121 gtggttgatg gcgaagaagt tacagaatac accacaaaag atggaaacgt gattcagatt
   102181 cctgcaggaa acgaagaagg catggaatca aaagatgctg gtggatcagg cgctcctgct
   102241 aaaaactaag ggttacaaca aagagcataa ggtgactcgc aaacgttacg catgacatga
   102301 ggcagtctgc ctaagatggt aactcactta tcataatgcc taaatagcca aacacactaa
   102361 ctcgcaaatg tacaaaatca tatacccgtg cttatgtgag cgaacggtta tatgattttt
   102421 gttttccaaa aacaagaaaa gaacgctaaa atagccagtt tgtcgtgttt caagctacga
   102481 aaaaggaagt gctaaaatga aaaaaagatc cattatacct gtcatcttac tgttggtcat
   102541 cggattaatc gttggtgtta agcgtcatca tgactagtag gtcttgctag tgaccgttca
   102601 aaagggataa cgttggtatg gtataaccat aaaaatcgta taaaaagaaa aaatagctat
   102661 tgaggagaaa gtatgtttca gttaagaaaa aaaatgacgc gcaaacaatt agccttgttg
   102721 agtgctggag tgttgacctg tgtggttggt ggtacctact tgataatgaa tcatcaacaa
   102781 caagaaattg tctctagtgt caacaaagta aaagccttaa ccataaaaga ggccatggaa
   102841 caaggaaaag atatcagctt gaccttagct ggcgaagtaa ccgctaacaa cagcagcaaa
   102901 gtcaaaatcg actcaagtaa aggagaagtc aaagatgtct ttgtcaaaaa aggcgatgtt
   102961 gtcaaagtag gacaaccctt gtttagctat gaaacgtcac aacggttaac ggctcaaagt
   103021 tcagaatttg atgttcaaac caaagccaat caactccaag ttgctaaaac caatgcagca
   103081 ttgaagtggg aaacctacaa tcgcaaggtc aatgagatca ataccctaaa atctcgctac
   103141 aacactgcac cagatgagag cttactagag caaattcgca gcgcagaaga cagtgtatcc
   103201 caagcactaa gcgatgccaa aacagcagat agcgatgtca aaaccgctca aatcgaactc
   103261 gataaagcta atgctactgc cacaatggaa aaaggtaaac tagagtatga caccgttaag
   103321 tcggacaccg caggaaccat tgttagccta aatactgatt tgccaaatca atcaaaatcc
   103381 aaaaaagaaa atgaaacttt tatggaaatt atcgacaaat caaaaatgtt agtcaaaggt
   103441 aacatcagtg aatttgaccg tgacaagtta aaaatcggtc aaaaagtcga agtgattgac
   103501 cgcaaagaca actctaaaaa atggactgga aaagtaaccc aagttggcaa cctcaaagca
   103561 gaggaaaaag gccaaggtca aggccaaggt ggcaatgacc aacaagacaa tccaaaccaa
   103621 gcaaaattcc cttatgttat tgaacttgac caatcagaca agcagccact cattggctca
   103681 cacacctatg ttaatgtgct caacaatgtt ccagaagctg gcaagatcgt attgaaagaa
   103741 acctttacaa tggcagaaaa tggaaaaacc tatgtgtgga aagttgataa aaacaaggtc
   103801 aaaaaacaag aagtcaagac taagcccttc tcaaaaggtt atattgaggt gacaagtggc
   103861 ttgactatgc aagataagat tgctcagccg cttcctggca tgaaagatgg tatggaggta
   103921 ggaagtattg ttaaacctta aagatattcg aaaaagctat catcttggga ctgaagaatt
   103981 tgcgatttta aaaggaatcg atttagaagt taacgagggt gactttttag ccatcatggg
   104041 accatcaggt tcgggaaagt caacattgat gaatatcatt gggtgtttag ataagcctgg
   104101 ctctggctca tatgccattg aaggcagaga cgtgtcatcc ttatctgata atgaacttgc
   104161 tgatttgcgt aatcaaaaaa tcggctttgt ttttcaaaac tttaacctga tgcccaagct
   104221 aacagcttgt caaaatgtcg aattgccctt gacttatatg aatgttccta aaaaagagcg
   104281 tcgcaaacga gccctagaga tgttaaaact agtaggacta gaagaacgta gtgaatttaa
   104341 accgatggag ctatctggtg ggcaaaaaca gcgtgtagcg attgcaagag ctttagtcac
   104401 taatccgagt tttatccttg gtgatgagcc aacaggtgca ctagacacaa aaaccagcgt
   104461 ccaaatcatg gacctattta aacaattcaa tgataacggc aaaacgatta ttatcatcac
   104521 acacgagcct gaagtagctg ccttatgcaa aaagacggtg atcctaagag atggtaatat
   104581 agaacattcc gatatagagt aaagggggaa aataatggaa gatattattg cagctttagg
   104641 ctctatatta tcacacaaga tgcggtcaat tttgacaatg ctagggatta ttatcggtat
   104701 tggtgccatt atcgcaatct tttcaatcat cgaaggtaat accgaaaata ccaaaagaca
   104761 gttgattggt ggcagcaata acactattaa cattgtgttt aacaaaaaaa gcagtattga
   104821 tcctaaattt ccagataagt caaatgctaa aaaaccagac tatcttcctt ttatggctga
   104881 agaagagctt agcaaaatac agcaagtcaa aggcgtcaaa aacgccctga tctcatatgg
   104941 tattgatgac aaagtctacc atctaggcca aaaatcatct gcaaagattt ctgccatcac
   105001 aaaaaacgta gctgaagtga gacgcatgac atttattaaa ggcagtgact tttcagacaa
   105061 agactttatt gaccaaaaac aggtgattta ccttgaaaaa agcttgtatg agtcgctatt
   105121 tccaaaagac gatggtcttg gaaaatttgt cgaagtgatg ggaaatccct tcagggtcat
   105181 tggggtgttt gaatcaaaag aacaaagtgg cctcacaagt gggactgaga aaattgctta
   105241 tatcccgcta catcagtggt ataatattaa tggtgtagtg gatgcgacac ctgagatcac
   105301 gatccagacc tatcgcgcag atgacctcaa acctgtggca aagcgtgtca gtgatatgct
   105361 caatcaaacg atccctaaat cagattatat gtttggtgtc atgaacctta aagaatttga
   105421 acgccaacta gacaacctaa acaaatctaa ttttgtgtta ttagctggta ttgctagcat
   105481 ctctcttatt gtaggtggta ttggtgtgat gaacattatg ctagtatcag ttaccgaaag
   105541 aacgagagaa attgggatca aaaaagcgct tggcgctaga cgtaagctta ttttaaaaca
   105601 atttttaatt gaagcggtca ttctaacctt gctaggtggt gtgatagggg ttatttcagg
   105661 gatggtatca ggattgatca tcacaagatc actcgagtat ccctatatcc tatccttgtt
   105721 ctcagttgtc ttgagtttag cgttttgttg tattataggg attgtctttg gactattacc
   105781 agccattaaa gcgtctaagc tagacccaat tgaagcattg cgatttgaat aatcaaccaa
   105841 acaagcataa aagtcaaaaa ggatgaccct ttgatgtcat ccttagctgc ttgatacact
   105901 aaacgccaat ctagaaaggg aagttatgtt taaaatatta gtagttgaag atgatgatac
   105961 catcagtcaa gtgatttgtg agtttttaaa agcgaataac tatgatccag attgtgtatt
   106021 tgatggccaa gctgccttag acaagtggca gacaacgtct tatgatctga ttattttaga
   106081 catcatgctc ccttccttaa gtggcttaga agtgctaaaa acaattcgaa agacatcaga
   106141 tgtccctatt attatgctaa cggcattaga tgacgagtac acacagcttg taagcttcaa
   106201 ccatttgatt agtgattatg tgactaaacc attttcacca ctcattttaa tcaaacgtat
   106261 cgaaaatgta ttacgcgtct ctactccaga tgaaaagcgc caaatcggag acttacttgt
   106321 tgatgagacc gaacatagcg tctattggca aggaacattg gtcaaactaa ccaaaaaaga
   106381 atatgacatt attgattatt tagcaaaacg tcaccaaaag attgtcacca gagaccaatt
   106441 gatggatgac atatggggct attctgaact ggacacgcgt gtccttgaca accacatcaa
   106501 gaaccttcgt aaaaaaatga cgggtattcc cctcaagaca attacaggca tgggctattt
   106561 attgggagag cgtgagtgag actaatcaaa aaaacctttt tggtcatcaa tggcttaatt
   106621 attgtagtgg ttacctctat tttgttagtg ctttattttg caatgccaat ttattacacc
   106681 aaagtcaaag acaaagaagt taagcgtgaa tttgaccaaa caagcaagca aatcaaaggc
   106741 aagacggtaa ccgagattag agatatccta acaaaaaaaa tcaacaaaga taatatttgg
   106801 tacagcttag tagacagtga taaccaactg ctctatcctt ccttgcaact gttagacggc
   106861 gtcagtgaaa gcaaagacag tcaaaatgtt aatatcgtca ccacctttga caactcttat
   106921 tctaatgtta aggtgatgag tcaaaaagta accctacgcg atggaaaaaa aatgacattg
   106981 cttgggcaat cttcacttca acccgtaaca gatgctagca aagttctcct tgacctctac
   107041 ccgtcactat tgattttttc agtgactgta gggagtattg tcgcctactt atatagtagg
   107101 acttctagcc ggcgtatcct tagcatgtca caaaccgcca aaaaaatggt caacctagaa
   107161 ccaaacttaa cgtgtaccat tcacggcaaa gatgaaatag caatgttggc tagtgatatt
   107221 aaccgtttgt atgcgagtct ttcaacgagc atcaaatcct tgcaaaaaga gtatgaaaaa
   107281 gcttctgatt cagaaagaga gaaatctgag tttttacgca tgacatcgca cgagctaaag
   107341 acaccaatca ccagtgttat cggaatgatt gatggaatgc tctataatgt tggtgacttt
   107401 gcagatcgtg acaaatattt gcgaaaatgc cgagacgtcc ttgaagggca agcccaactg
   107461 gttcaatcca ttttatcttt atctaagatt gaaaccctag cttctcaaaa tcaagaactg
   107521 ttttcactaa aaagcagcct agaagaagaa atggaagtct ttctcgtctt atcagaacta
   107581 aaacacctca aagtgactat caatctcgaa gaacaattcg tcaaagccaa taaagtatac
   107641 ctactaaagg caattaaaaa tattattgac aatgcctttc actataccaa accaggcggc
   107701 caagtgatga ttcaactaaa agacaaccaa ctagtgatta aaaatgaagc agagacatta
   107761 ttgacacaac agcagatgaa acagttattc caaccgtttt atcgaccaga ttatagtcgt
   107821 aacaggaaag acggtggtac aggattgggg ctgtttatca cccaccagat tcttgatcag
   107881 catcatctgg cttatcgctt tgttgttctt gatcaaagat ggatggtatt tacgattgat
   107941 tttccatccc atcatgacga ttgatccaca ctagtaggac gacaaaagtc atcaatcact
   108001 aaccaagcct tgacctttac ccatcattat ttaggcaagt ataaaaaaga ggagagaaat
   108061 catatatgaa gaaaaggaaa ttgttagcag taacactatt aagtaccata ctcttaaaca
   108121 gtgcagtgcc attagttgtt gctgacacct ccttgcgtaa taacccatca tccactgatc
   108181 agcctactac agcagatact gatacggatg acgagagtga aacagcaaaa aaagacaaaa
   108241 aaagcaagga aacagcgtcg cagcacgaca cccaaaaaga ccataagcca tcacacaatc
   108301 acccaacccc cccttcaaat gatactaagc agaccgatca ggcatcatct gaagctactg
   108361 acaaaccaaa taaagacaaa aacgacacca agcaaccaga cagcagtgat caatccaccc
   108421 catctcccaa agaccagtcg tctcaaaaag agtcacaaaa caaagatggc cgacctaccc
   108481 catcacctga tcagcaaaaa gatcagacac ctgataaaac accagaaaaa tcagctgata
   108541 aaacccctga aaaaggacca gaaaaagcaa ctgaaaaaac tccagagcca aatcgtgacg
   108601 ctccaaaacc catccaacct cctttagcag cagcagctcc tgtctttgga cttttgagag
   108661 aaagtgacaa agacctgagc aagctaaaac caagcagccg ctcatcagcc gcttacgtga
   108721 gacactggac aggtgactct gcctacactc acaacctgtt gtcacgccgt tatgggatta
   108781 ctgctgaaca gctagatggt tttttgaaca gtctaggtat tcactatgat aaagaacgct
   108841 taaacggaaa gcgtttatta gaatgggaaa aactaacagg actagacgtt cgagctatcg
   108901 tagctattgc aatggcagaa agctcactag gtactcaggg agttgctaaa gaaaaagggg
   108961 ccaatatgtt tggttatggt gcccttgact tcaacccaaa caatgccaaa aaatacagcg
   109021 atgaggttgc tattcgtcac atggtagaag acaccatcat tgccaacaaa aaccaaacct
   109081 ttgaaagaca agacctcaaa gcaaaaaaat ggtcactagg ccagttggat accttgattg
   109141 atggtggggt ttactttaca gatacaagtg gcagtgggca aagacgagca gatatcatga
   109201 ccaaactaga ccaatggata gatgatcatg gaagcacacc tgagattcca gaacatctca
   109261 agataacttc cgggacacaa tttagcgaag tgcccgtagg ttataaaaga agtcagccac
   109321 aaaacgtttt gacctacaag tcagagacct acagctttgg ccaatgcact tggtacgcct
   109381 ataatcgtgt caaagagcta ggttatcaag tcgacaggta catgggtaac ggtggcgact
   109441 ggcagcgcaa gccaggcttt gtgaccaccc ataaacctaa agtgggctat gtcgtctcat
   109501 ttgcaccagg ccaagcagga gcagatgcaa cctatggtca cgtcgctgtc gtagagcaaa
   109561 tcaaagaaga tggttccatc ttgatttcag agtcaaatgt tatgggatta ggcaccattt
   109621 cctatcggac gttcacagct gagcaggcta gtttgttgac ctatgtcgta ggggacaaac
   109681 tcccaagacc ataaccccac aaccaagaag ctacctgcct gttgaccaat ctcaaaaaga
   109741 gatagcgtca acttagaatg aggttcatca gatgataaag aaagtgacca ccccgagtca
   109801 aaaaacaaaa aaacgtgttc gtaatggata cctcctaaaa gtaggtacag cctgtttact
   109861 cttaagtata ctcagttatg gcattggtct tttgggccag cctagcatgg aaaatacgtt
   109921 tatgggaata gcgagtgttg ccatgttagg aagtgtctgt ttctttatta tctttgctct
   109981 aaaccgtatc tttgacgcac tagaggacaa tttgcgagat tagagtaata ggtcaaataa
   110041 tcttccaata atttagagca caagctaagt tattggaagt ttttttgtct tttgagagcc
   110101 atgatgccat caaaaagcca aacactcctt atgatgtgag ggtcacatgg ctaaaattaa
   110161 aagcaaaata gaccccaaag ccctgttttc tcaaaacagc caacgaaaaa agacaagtta
   110221 accagttcat aaaaaacatc gtagaaacgt tttcaagagc taatgttgga gtaaattgac
   110281 tgaagtatga tagaattttt aatggttgta ccataacagt caaacttaaa aacggaaatc
   110341 agacaaaaac attaaattgc atgtaaaaaa tctaattaag ttagtgacaa aagataaatt
   110401 aggttcaaaa aatataaaaa aaatataaat taagacctct tttaaaacaa aaagagcata
   110461 aatggcatga aaaatgggta aaa
//
LOCUS       DAUDYX010000002        69712 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.002, whole genome shotgun sequence.
ACCESSION   DAUDYX010000002 DAUDYX010000000
VERSION     DAUDYX010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 69712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69712
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.002"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            60..1424
                     /gene="ftsA"
                     /locus_tag="VHD02_000100"
     CDS             60..1424
                     /gene="ftsA"
                     /locus_tag="VHD02_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEQ5496741.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFISGEMNVIGVSNVPSTGVKD
                     GIIIDIEAAATAIKTAVEQAEEKAGMTIEKVNVGLPANLLQIEPTQGMIPVPSESKEI
                     KDEDVDSVVKSALTKSITPEREVISLVPEEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPSTILHNLRKTVERAGIKVENIIISPLAMAKTILNEGEREFGATVIDMGGGQTTVAS
                     MRAQELQYTNIYAEGGEYITKDISKVLKTSLAIAEALKFNFGQAEISEASITETVKVD
                     VVGSEEPVEVTERYLSEIISARIRHILDRVKQDLERGRLLDLPGGIVLIGGGAIMPGV
                     VEIAQEIFGVTVKLHVPNQVGIRNPMFSNVISLVEYVGMMSEVDVLAQTAVSGEELLR
                     RKPIDFSGQESYLPDYDDSRRPESTIGYEQQASQTAYDSQVPSDPKQKISERVRGIFG
                     SMFD"
     gene            1448..2767
                     /gene="ftsZ"
                     /locus_tag="VHD02_000101"
     CDS             1448..2767
                     /gene="ftsZ"
                     /locus_tag="VHD02_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEQ5496742.1"
                     /translation="MAFSFDTASIQGAIIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEILTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVVTRPFGFEGNKRGNFAIEGIEELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITSPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERIVEAARKAIYSPLLETTIDGAQDVIVNVTGGLDMTLTEA
                     EEASEIVGQAAGQGVNIWLGTSIDDTMKDDIRVTVVATGVRQEKAEQVSGFRQPRTFT
                     QTNAQQVAGAQYASDQAKQSVQPGFDRRSNFDFDMGESREIPSAQKVISNHNQNQGSA
                     FGNWDLRRDNISRPTEGELDNHLNMSTFSANDDSDDELETPPFFKNR"
     gene            2770..3441
                     /locus_tag="VHD02_000102"
     CDS             2770..3441
                     /locus_tag="VHD02_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEQ5496743.1"
                     /translation="MDLLTNKKKIFETIRLSTEAANRTNDSVSVIAVTKYVDSTIAGQ
                     LIEAGIEHIAENRVDKFLEKYDALKCMPVKWHLIGTLQRRKVKEVINYVDYFHALDSV
                     RLALEINKRADHPVKCFLQVNISKEESKHGFNISEIDEAIEEIGKMEKIQLVGLMTMA
                     PANASKESIITIFRQANQLRKNLQLKKRKNMPFTELSMGMSNDYPIAIQEGSTFIRIG
                     RAFFH"
     gene            3453..4109
                     /locus_tag="VHD02_000103"
     CDS             3453..4109
                     /locus_tag="VHD02_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEQ5496744.1"
                     /translation="MAFKDTFNKMISYFDTDEVNEVEEDVAASTDNVIPRSQQSVRAS
                     SHPKQEPRNNHVQQDHQARSQEQTRSQMHPKHGTSERYYQQSQPKEGHEMVDRRKRMT
                     TSGIANRREQYQQSTCSDQTTIALKYPRKYEDAQEIVDLLIVNECVLIDFQFMLDAQA
                     RRCLDFIDGASKVLYGSLQKVGSSMYLLAPSNVSVNIEEMTIPHTTQDIGFDFDMKRR
                     "
     gene            4114..4368
                     /locus_tag="VHD02_000104"
     CDS             4114..4368
                     /locus_tag="VHD02_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEQ5496745.1"
                     /translation="MILILSILLRLIKVYTYLLIAYALMSWFPGAYDSKIGRLISGIV
                     EPILKPFRAFNLQFAGLDFTIFVVIISLNFLAQVLVRVFI"
     gene            4368..5159
                     /locus_tag="VHD02_000105"
     CDS             4368..5159
                     /locus_tag="VHD02_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEQ5496746.1"
                     /translation="MISHSKIYQHFHQEEYPFIDRMSDMINRVEDYYLLEVTEFLNPR
                     EVMILKSLIALTDLKMFVSTDYYPSEYGRVIIAPGYYDLEQSDFQIALVEISYQAKFN
                     QLTHSQILGTLINELGVKRNLFGDVFVEMGYAQLMIKRELLDYFLGTITKIAKTSVKL
                     REVNFDQLIRSIDNSQTLDILVSSFRLDGVVATILKKSRTQVIALIEANKIKVNYRLA
                     NKASDNLVIGDMVSIRGHGRFTLLADNGVTKHGKQKITLSKMIHK"
     gene            5169..5927
                     /locus_tag="VHD02_000106"
     CDS             5169..5927
                     /locus_tag="VHD02_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEQ5496747.1"
                     /translation="MALTTLEIKDKTFKTKFRGYCEEEVNEFLDIVVDDYEALVRKNR
                     DNEARIKDLEEKLSYFDEMKESLSQSVILAQETAEKVKATANAEATNLVSKATYDAQH
                     LLDESKAKANQMLRDATDEAKRVAIETEELKRQTRVFHQRLISSIESQLSLSNSPEWD
                     ELLQPTAIYLQNSDDAFKEVVKTVLNEDIPESDDSASFDATRQFTPEELEELQRRVDE
                     SNKELEAYQLDSQSDSTTEPEVNLSETQTFKLNI"
     gene            6200..9001
                     /locus_tag="VHD02_000107"
     CDS             6200..9001
                     /locus_tag="VHD02_000107"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEQ5496748.1"
                     /translation="MKLKETLNLGKTAFPMRAGLPNKEPQWQAAWEQAELYKKRQELN
                     AGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFQAPYVPGWDTHGLPIE
                     QVLAKQGIKRKEMDLAEYLEMCRQYALSQVDKQRDDFKRLGVSADWENPYVTLDPQFE
                     ADQIRVFGAMAEKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTNTYIVVWTTTPFTVTASRGLTVGPDMDYLVVKPAGSDRQYVVAEGLLDSLAGK
                     FGWESFETLASHKGADLEYIVTEHPWDTDVEELVILGDHVTLESGTGIVHTAPGFGED
                     DYNVGTKYKLEVAVTVDERGLMMENAGPDFHGQFYNKVTPIVIDKLGDLLLAQEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSDFRQDILDEIEKTTFHPSWGETRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFQENGSIIWWQKEAKDLLPEGF
                     THPGSPNGEFTKETDIMDVWFDSGSSWNGVMNARENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAILSQGFVLDGKGEKMSKSKGNIISPNDVAKQYGADILRLWVAS
                     VDTDNDIRVSMEILGQVSETYRKIRNTLRFLIANTSDFNPATDTVAYADLGAVDKYMT
                     IVFNQLVATITDAYERYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSLERRR
                     MQTVFYDILVKITKLLTPILPHTTEEIWSYLEYESEAFVQLAEMPVAETFSAQEDILE
                     AWSAFMTLRTQAQKALEEARNAKIIGKSLEAHLTIYASEEVKTLLTALDSDIALLLIV
                     SQLTIADLADAPADAVAFEGVAFMVEHAIGEVCERSRRIDPTTRMRSYNAFVCDHSAK
                     IIEENFPEAVAEGFEESGK"
     gene            complement(9265..9567)
                     /locus_tag="VHD02_000108"
     CDS             complement(9265..9567)
                     /locus_tag="VHD02_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEQ5496749.1"
                     /translation="MRLINTTSSHPELIRNQLKNTDAYLVEVYSAGNTDVIFTQAPKH
                     YELLISNKYRAIKEDELDIIREFFLKRKIDPKIVIPGQSKTLHTNNLIEISFQTSV"
     gene            complement(9618..10073)
                     /locus_tag="VHD02_000109"
     CDS             complement(9618..10073)
                     /locus_tag="VHD02_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEQ5496750.1"
                     /translation="MMIPTFGHKNAHKDYVTRYGVYAIIPNHEQTKIILVQAPNGSWF
                     LPGGEIEAGEGQLQALERELIEELGFSATIGSYYGQADEYFYSRHRDTHFYHPAYLYE
                     VTAFQAVSKPLEDFNNLGWFSPIEAIAKLKRESHQWGVKEWQKKHHSTN"
     gene            complement(10201..12483)
                     /locus_tag="VHD02_000110"
     CDS             complement(10201..12483)
                     /locus_tag="VHD02_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEQ5496751.1"
                     /translation="MLCQNCNLNESTIHLYTSVNGKQRQVDLCQNCYQIMKSDPANSI
                     LNGLTPGYRAQDRSTSPFFDDFFGDLNNFRAFGNLPNTPPTQAGQNGNGGGRYGGNYN
                     GQRPAQPQTPNQQAKGLLEEFGINVTDIARNGNIDPVIGRDEEITRVIEILNRRTKNN
                     PVLIGEPGVGKTAVVEGLAQKIIDGTVPQKLQGKQVIRLDVVSLVQGTGIRGQFEERM
                     QKLMEEIRNRKDVILFIDEIHEIVGAGSAGDGNMDAGNILKPALARGELQLVGATTLN
                     EYRIIEKDAALERRMQPVKVDEPSVEETITILKGIQPKYEDYHHVKYSPAAIEAAAHL
                     SNRYIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDKRLIEAENLKAQATRDEDYE
                     RAAYFRDQIAKYKEMQAQKVDEQDIPIITEKTIEAIIEQKTNIPVGDLKEKEQSQLVN
                     LANDLKAHVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLA
                     IELFGSADNMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEQVRRNPYSLILLD
                     EVEKAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGTGKSEASVGFGAAR
                     EGRTSSVLGELSNFFSPEFMNRFDGIIEFKALSKEHLLHIVDLMLEDVNERLGYNGIH
                     LDVTQKVKEKLVDLGYDPKMGARPLRRTIQDYIEDAITDYYLEHPTEKQLRALMTNSE
                     NITIKAVKEGDSFLNEESLD"
     gene            12781..13011
                     /locus_tag="VHD02_000111"
     CDS             12781..13011
                     /locus_tag="VHD02_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEQ5496752.1"
                     /translation="MESHLVRIINRLELMVTDGGNLKRNFERDGVVVAEVAFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            13138..13824
                     /locus_tag="VHD02_000112"
     CDS             13138..13824
                     /locus_tag="VHD02_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ5496753.1"
                     /translation="MDLSFLPKYWAYFNYGVLVTIMISVSVVFFGTLIGVLVTLIKRS
                     HVKPLTWVVNLYVWIFRGTPMVVQIMIAFAWMHFNNMPTIGFGVLDLDFSRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPKGQLEAAYSLGIRPQNAMRYVILPQAFKNILPALGNE
                     FITIIKDSALLQTIGVMELWNGAQSVVTATYSPISPLLVAAFYYLMVTTVMAQLLAVL
                     ERHMAQGGNH"
     gene            13824..14558
                     /locus_tag="VHD02_000113"
     CDS             13824..14558
                     /locus_tag="VHD02_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ5496754.1"
                     /translation="MTEAIISIKDLHKYYGHNEVLKGIDLDIMPGEVVVIIGPSGSGK
                     STLLRTMNLLEVPTKGQIRFEGIDITDKKNDIFSMREKMGMVFQQFNLFPNMTILENI
                     TLSPIKTKGMAKAEADKTALSLLDKVGLSEKAKAYPASLSGGQQQRIAIARGLAMDPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAKEVADRVIFMDGGVI
                     VEEGSPNQLFDLTKEERTKDFLSRVL"
     gene            14707..16116
                     /locus_tag="VHD02_000114"
     CDS             14707..16116
                     /locus_tag="VHD02_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEQ5496755.1"
                     /translation="MVSVMWFRRDLRIDDQKALAHAIASQSPTLCVFQFNKEQLNLLH
                     SRNQSSFITSVLAFRKLLKCEGIDLYLMYGDLMTCFEQLLTQLKDWTDVYFNYDESGY
                     GRLRDQKAAQFFKKNGIAVHTYQDHYLHGSQEIINQSGQPYKVFTPYYRIWQNYPKET
                     PIKVELSQGRWLNLETPDDVLRTVESFKDEKYQDVATFDEASKQLNRFIQDQLAAYHA
                     NRDFPAQLGTSRLSPFLRIGAIGIRTVYHAVRQAPNSLGQATFLKELAWRDFYNMVYV
                     AYPDQKTQPIQKAFSQIEWVNNPDWFQLWKEGKTGYPIVDAAMLQLQKTGWMHNRLRM
                     IVASFLTKDLLCDWRLGEQHFQQQLIDYDAASNIGGWQWAASTGTDAVPYFRVFNPVT
                     QSKRFDPKGEFIKAYLPQLEHVPEKYLHEPWKMPKYLQESVSCIIGTDYPQPIVDHAK
                     QREQAIAKYEWAKEKAKIE"
     gene            16294..17988
                     /locus_tag="VHD02_000115"
     CDS             16294..17988
                     /locus_tag="VHD02_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEQ5496756.1"
                     /translation="MTYNEVYQEWLHNNDLSDDIKADLAAIKDNEAEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAVKKGIAVSYDVRYQSRTFA
                     ELTCSIMAANGIKAYLYKGIRPTPMCSYAIRALGCISGVMITASHNPQAYNGYKAYWQ
                     EGSQILDDIADQIAQHMAALTQYQEIKQMPFEKALDSGLVTYIDESIEEAYKKEVLGL
                     TINDTDIDKSVRVVYTPLNGVGNLPVREVLRRRGFENVYVVPEQEMPDPDFTTVGYPN
                     PEVPKTFAYSEKLGKAVDADILIATDPDCDRVALEVKNAVGDYVFLNGNKIGALLSYY
                     IFSQRFDLGNLPANPVLVKSIVTGDLSRAIASHYGIETVETLTGFKNICGKANEYDVT
                     KQKNYLFGYEESIGFCYGTFVRDKDAVSASMMIVEMAAYYKKKGQNLLDVLQTIYATF
                     GYYNERQIALELEGIEGQKRIARIMEDFRQTPIASVAEMALDKTIDFIDGYQDFPKQN
                     CLKFYLDDGSWYALRPSGTEPKIKFYLYTIGQTQENSATKLDAIEAACRTKINQVN"
     gene            18196..19050
                     /locus_tag="VHD02_000116"
     CDS             18196..19050
                     /locus_tag="VHD02_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEQ5496757.1"
                     /translation="MTELIDGKALAQKMQQELAAKVNNLKQKKGIVPGLAVILVGDDP
                     ASQVYVRNKERAALTVGFKSETVRLSEFICQEELIAVIERYNADNTIHGILVQLPLPN
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPLMVPCTPSGIMELLREYNVNLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLEEVCRCADVLIVAIGQGHFITKQ
                     YIKEGAIVIDVGMNRDDNGKLIGDVAFDEVAEVAAKITPVPGGVGPMTIAMLLEQTYQ
                     SALRSTHK"
     gene            19203..20543
                     /locus_tag="VHD02_000117"
     CDS             19203..20543
                     /locus_tag="VHD02_000117"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEQ5496758.1"
                     /translation="MADYLTVTHLTKYLKLKFDRDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDESAVIQATMWAGVYKKLGFDLEEGMKINVIGRVQLYEPSGSYSIVIEKAEPDG
                     IGALALQFEQLKKKLTAEGYFEQKHKQPLPQFVSKIGVITSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGDGAAQEVVANIRRANQREDLDLLIVGRGGGSIEDLWAFNEEIVV
                     QAIFESQLPVISSVGHETDTTLADFVADRRAATPTAAAELATPITKTDLMSWIVERQN
                     RSYQACLRRIKQRQEWVDKLSQSVIFRQPERLYDAYLQKIDRLSMTLMNTMKDRLSSA
                     KENKVQLDHALANSQLQTKIERYQDRVATAKRLLMANMASQYDSQLARFEKAQDALLS
                     LDTSRIIARGYAMIEKNQALVASVSQITKGDQLTIKMRDGQLDVEVKDVKNENI"
     gene            20521..20736
                     /locus_tag="VHD02_000118"
     CDS             20521..20736
                     /locus_tag="VHD02_000118"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEQ5496759.1"
                     /translation="MSKTKTFEENLQDLETIVNKLENGDVPLEEAISEFQKGMLLSKE
                     LQKTLQAAEKTLVKVMQADGTEVDMDD"
     gene            20736..21608
                     /locus_tag="VHD02_000119"
     CDS             20736..21608
                     /locus_tag="VHD02_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEQ5496760.1"
                     /translation="MDKLARIDEAIRRYYKTTSNGVSEELIDAILYSVDSGGKRIRPL
                     ILLEMIEGFGVSLQNAHFDLAAALEMIHTGSLIHDDLPAMDNDDYRRGRLTNHKQFGE
                     ATAILAGDSLFLDPFGLIAQAELNSEVKVALIQELSLASGTFGMVGGQMLDMKGENQA
                     LSLPQLSLIHLNKTGKLLTFPFKAAALVTEQAITVRQQLEQAGMLIGHAFQIRDDILD
                     VTASFEDLGKTPKKDLFAEKATYPSLLGLEASCQLLTESLDQALTIFQTLESDVGFKP
                     QIITKLIEGLRLNV"
     gene            21601..22428
                     /locus_tag="VHD02_000120"
     CDS             21601..22428
                     /locus_tag="VHD02_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEQ5496761.1"
                     /translation="MSKERVDVLAYKQGLFETREQAKRGVMAGLVVSVINGQRYDKPG
                     DKIDDGTELKLKGEKLKYVSRGGLKLEKGLHVFGVSVANQIGIDIGASTGGFTDVMLQ
                     DGAKLVYAVDVGTNQLVWKLRQDPRVRSMEQYNFRYAQPEDFNEGQPVFASIDVSFIS
                     LSLILPALHNVLSDQGQVIALIKPQFEAGREQIGKKGIVKDKQIHEKVIQKVMDFASG
                     YGFTVKELDFSPIQGGHGNIEFLAHLVKSQTPETLAPHLIQEVVAKAHKEFEKHEKK"
     gene            22415..22885
                     /locus_tag="VHD02_000121"
     CDS             22415..22885
                     /locus_tag="VHD02_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEQ5496762.1"
                     /translation="MKKSERLELIKKMVLTHPIETQHDLLRLLAEHGLELTQATISRD
                     MNEIGIVKIPSGSGRYIYGLSQDSGKKIVQGPRSIKSTILAVSDKTKGLEQHLYLKVV
                     PGNSKLIKRYLLADFSKAIFSLIADDDSLLLIAKSPSEADMIRQEILLWMQGIT"
     gene            22906..24567
                     /gene="recN"
                     /locus_tag="VHD02_000122"
     CDS             22906..24567
                     /gene="recN"
                     /locus_tag="VHD02_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEQ5496763.1"
                     /translation="MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNM
                     MLGARASTEVIRRGANKAEIEGFFSVDATPELVACLESSGIAMEEELIIRRDIFANGR
                     SVSRINGQMVNLATLKQVGQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKEN
                     YQLIFDRYKSLRRQVIDKQKNEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDR
                     LMNHKQIADTLTNAYVMLDNDDFSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYY
                     ILEEVSKQLSDTIDQLDFDGGRLQEIEFRLDTLNSLTRKYGGNVNDVLDYYDNIIKEY
                     QLLTGDDLSSGDLEAELKSLEKQLVAAASKLSVSRHQLAEQLEAEIKAELKELYMEKA
                     DFKVHFTTSKFNRDGNESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIYKIGRHGQVLAISHLPQVIAIADYQYFISKES
                     KEESTVSKVRLLTPEERVEEIASMIAGTDMTQAALTQARELLAKH"
     gene            24739..25668
                     /locus_tag="VHD02_000123"
     CDS             24739..25668
                     /locus_tag="VHD02_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEQ5496764.1"
                     /translation="MTSKKACLSSIIVLASLTCGNDTVSANHLSATGDKFDDCSTLVE
                     KDVAPKDELEMLAWSSSQTTDDADRDYEDFLDDDSFNDDSFISQNETDKMFENLTDDR
                     LLDELDEEDTIEPEQNVIMPSDDELFDLTDAVETRLTVSSAPHLEAELPKPHLRSLSD
                     TALRSGEIRGHLDNKLDALSVTATKLALTMAQKFDLTTHVYSIGESFSEVLAAHYEDR
                     KAESAFSKKKRFHLPIATPDVVIEELRRLVSSIGSSKEDVSVPYNRKLGMAVAKRKIA
                     LPQTGEEFSYYPALLGLMILGLTPIMIPKKINN"
     gene            25884..26735
                     /locus_tag="VHD02_000124"
     CDS             25884..26735
                     /locus_tag="VHD02_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ5496765.1"
                     /translation="MEKYMGTIKIVTDSSITIEPELIKALDITVVPLSVMIDSKLYSD
                     NDLKEEGHFLSLMKASKSLPKTSQPPVGLFAETYENLVKKGVTDIVAIHLSPALSGTI
                     EASRQGAEIAEAPVTVLDSGFTDQAMKFQVVEAAKMAKAGASLNEILAAVQAIKSKTE
                     LYIGVSTLENLVKGGRIGRVTGVLSSLLNVKVVMALKNDELKTLVKGRGNKTFTKWLD
                     SYLAKNSHRPIAEIAISYAGEASLALTLKERIAAYYNHSISVLETGSIIQTHTGEGAF
                     AVMVRYE"
     gene            26728..27570
                     /locus_tag="VHD02_000125"
     CDS             26728..27570
                     /locus_tag="VHD02_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEQ5496766.1"
                     /translation="MNNRHLFSGIFFFVISLCLAFLLLNIIIPKSNSRLKKSDFLTKE
                     QVAIQYVAIGDSLTEGVGDLTHQGGFVPLLTNDLSEYFKANVNHQNYGVSGDTSQQIL
                     DRMIKQKQIQLSLKKADIMTLTVGGNDVMAVIRKNLADLQVSSFRKPARQYQKRLRQI
                     IELARKDNKDLPIFILGIYNPFYLNFPELTDMQKVIDDWNIKTKEVVGEYDRVYFVPI
                     NDLLYKGINGQEGIVHSSGDQTTIVNDALFTGDHFHPNNTGYQIMSNAVMEKIKKHEK
                     KIKP"
     gene            27548..28135
                     /locus_tag="VHD02_000126"
     CDS             27548..28135
                     /locus_tag="VHD02_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEQ5496767.1"
                     /translation="MKKKSNLNWWKWSFLCLLAFNTAFLMVIASRLIQVREPESELIA
                     KKPVKNIKIGTFVTTREQLNETVASYLKDYQTKKMSYKFYATSSSILFEGTYQLLGYE
                     VPLYIYFQPHRLENGAVQLQVISFSVGTLPLPEKDVLQYLKSSYKLPSFVKVMPNQSA
                     IVVNLQDIQNDAKVYLKAKKIDLFNDEISFNIYKK"
     gene            28234..28509
                     /locus_tag="VHD02_000127"
     CDS             28234..28509
                     /locus_tag="VHD02_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEQ5496768.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFSTIEAFLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
     gene            28854..30716
                     /gene="cadA"
                     /locus_tag="VHD02_000128"
     CDS             28854..30716
                     /gene="cadA"
                     /locus_tag="VHD02_000128"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEQ5496769.1"
                     /translation="MTIRQWMADHLHLMETLACLVLIIIGLAFLHSFPQVASAIFITA
                     FLIGGYASAKTGILDLVKNKHLSVDILMILAAIGAGIIGYWLEGALLIFIFSLSNTLE
                     EMAMEKSKDAISALMSLTPDTARQYQEDGHILEVETRSLSVGDRLQVRKGEAVPIDGQ
                     LLSPFGQFDESMVTGEPITVDKAEGQDLIGGTINQGQTIDMLVTIENDDTLFAKIINL
                     VESAQEKKSKTATFIESLEDGYVKFVLVLIPAFILFSHFVFSWTWLAAFYRGMILLTV
                     ASPCALIASSTPASLAAISRAARKGLIIKGGDIVDNMGDIKAVVMDKTGTLTQGKPSV
                     VNAHYLEDELLVNRLVKGAEAASTHPISKALLEYTEKLEPLTFDHLEEISGKGFQGFY
                     QGQEWRIGKKTFILEKVQDLSAFEETIQVEENQGKTLIFVSRDHQLIAYYALLDDIKI
                     ESKRAIKSLHAMGIKTVMLTGDQERTANYVAQKLGIDEVVANCMPQDKVAKLAELKTK
                     YGFVAMVGDGINDAPALAQADVSYAIGSGTDIAMESADSVIMDDLTRIPFSIQLSRKM
                     KTIIKQNIVFALSVITLLILANVFQVVNLPLGVVGHEGSTILVILNGLRLLSFK"
     gene            complement(31016..31951)
                     /locus_tag="VHD02_000129"
     CDS             complement(31016..31951)
                     /locus_tag="VHD02_000129"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEQ5496770.1"
                     /translation="MVSTATQISHFSFDNCLMNAAGVYCMTKEELMEVEKSQAASFVT
                     KTGTLEVRPGNPEPRYADTRLGSINSMGLPNNGFRYYLDFVSDLAKTGQHKPHFLSVV
                     GLSPTETETILKAIMASDYEGLVELNLSCPNVPGKPQIAYDFETTDQLLENIFTYYTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLSFVNCVNSIGNGLVIEDEQVLIKPKNGFGGI
                     GGDYIKPTALANVHAFYKRLKPSIHIIGTGGVKTGRDAFEHILCGASMVQIGTALHQE
                     GPAIFERVTKELKTIMVEKGYQSLDDFRGNLRYKD"
     gene            32190..32885
                     /locus_tag="VHD02_000130"
     CDS             32190..32885
                     /locus_tag="VHD02_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEQ5496771.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDLAFTSVLTRAIKTTNLALENAGQLWVPTEKSWRLNERHYGALTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPAMAKDDEYSAHKDRRYADLDPALIPDAENLKVTLERAM
                     PYWEEKIAPALLDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFELDE
                     KLNIVKEYYLGGE"
     gene            32993..33514
                     /locus_tag="VHD02_000131"
     CDS             32993..33514
                     /locus_tag="VHD02_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEQ5496772.1"
                     /translation="MKAEDRRQKIIECLNSEQKAVSATRLGKLLGVSRQVIVGDIALL
                     RAQQIDIISTPKGYIMSTALYSHQFCARIVCQHNVEETKKELEIILAHQGIITTVEVE
                     HPIYGMITAPLNIKTHSDVTNFMSKLSQSKAELLSSLTEGLHSHLISCPSQEAFLAIK
                     HDLELAGILYKGI"
     gene            33675..34217
                     /locus_tag="VHD02_000132"
     CDS             33675..34217
                     /locus_tag="VHD02_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496773.1"
                     /translation="MTKKPTQLIAYTSILVAFAILIPIIMPLKLIIGPASFTLASHVP
                     LFLAIFMSIPVAILVALGTTLGFLLAGLPLIIVLRALSHLLFAILAAWWLSRKPQLMT
                     SAVKCFSFAFFINVIHGLAEFLVVYILTATTATSMSYFWSMLGLIGLGSLIHGILDFY
                     LALVLWRFLAKNLKLPTINA"
     gene            34341..35120
                     /locus_tag="VHD02_000133"
     CDS             34341..35120
                     /locus_tag="VHD02_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEQ5496774.1"
                     /translation="MKTPEQILEATIHIGEHKVTKTFLAKSILGFIGGAMISLGYLLY
                     VRIAASGLETFGAFSSIVGACAFPIGLIIILMAGGELITGNMMAVSAALLAKKIKFSE
                     LAKNWLIITLFNVIGAVFVAFVFGHFLGLTSAGIFKEEVIEVAHAKIAASPLQALVSG
                     IGCNWFVGLALWLCYGANDAAGKFLGTWFPVMTFVALGFQHSVANAFVIPAAIFEGGA
                     TWLDFVTNFIFVYSGNIIGGAIFVSFLYFKVYYHPQKSKTQ"
     gene            35908..36504
                     /gene="recR"
                     /locus_tag="VHD02_000134"
     CDS             35908..36504
                     /gene="recR"
                     /locus_tag="VHD02_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEQ5496775.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSNEDVNDFA
                     KNLLAAKRELTYCSICGNLTDDDPCHICTDTSRDQTTILVVEDAKDVSAMEKIQEYHG
                     YYHVLHGLISPMNGVGPDDINLKSLITRLMDGKVSEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            36605..37651
                     /locus_tag="VHD02_000135"
     CDS             36605..37651
                     /locus_tag="VHD02_000135"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEQ5496776.1"
                     /translation="MSKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFIT
                     QMGQFIKTQQFSEKPSESERLMTNETIELTQKIKPSDIYEEGAVVFPVLHGPMGEDGS
                     IQGFLEVLRMPYIGTNVMSSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVE
                     TLARLTFPIFVKPANMGSSVGISKAQTKVELRKAIQLALTYDSRVLIEQGVVAREIEV
                     GLLGNDKVKSTLPGEVIKDVDFYDYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAF
                     KALGGCGLSRCDFFLTQDGQVYLNELNTMPGFTQWSMYPLLWENMGLAYPDLIEELVT
                     LAQEMFDQRESHLI"
     gene            37850..39241
                     /locus_tag="VHD02_000136"
     CDS             37850..39241
                     /locus_tag="VHD02_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEQ5496777.1"
                     /translation="MKLTLHEVAKIVDAQNNVSDLDDVPLHHIEFDSRKITKGDLFLP
                     LKGQRDGHEFIDLAFQNGAVATFSEKELPGTPHLLVEDCLKAFQKLAHYYIDKMRVDV
                     IAVTGSNGKTSTKDMIGAVLSTTYKTYKTQGNYNNEIGLPYTVLHMPDDTEKIVLEMG
                     QDHMGDIRLLSEIARPRIAVLTLVGEAHLEYFGSRDKIAQGKMQIVDGMNSDGILIAP
                     GDPIIDPYLPENQMVIRFGNQQEIDVTGIQEDKDSLTFTTNVLATPVSLPLPGKYNAT
                     NAMVAAYVGKLLAVTDEDIIAALQTVTLTGNRTEWKKAANGADILSDVYNANPTAMRL
                     ILETFANIAKNPGGKKIAVLADMKELGKDSVILHSQLIDSLTSGNIDQLVFYGDHIKE
                     LARLASQVYPAEQVHYFLKTEQEDQFEAMAQYVQNILNPFDQILLKGSHSMSLEKLVE
                     RLVTDKNCDSYQP"
     gene            39322..40017
                     /locus_tag="VHD02_000137"
     CDS             39322..40017
                     /locus_tag="VHD02_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEQ5496778.1"
                     /translation="MDFFAIDPIGLPHISLIFYLLSLLIALLLVFLTFQAYRLKSYRY
                     FFLFLQLSQVIGLYTWYVLRGFPLDEALPLYHCRIAMLAIFFLPDRNKFKQLCMVLGI
                     GGTFLALLSPDLYPFRLWHVANVSFYLGHYALLVNGLIYLLRFYDASQLRLLSVVRYL
                     ATVNFLLLLVSLATKGNYGFVMDIPVIHTRHLLLNFVIVTSGLTFMVKITEYFYLKFG
                     EAQQLAFAFSKEK"
     gene            40265..41809
                     /locus_tag="VHD02_000138"
     CDS             40265..41809
                     /locus_tag="VHD02_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEQ5496779.1"
                     /translation="MSLTEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLG
                     IASYPMNWPIGMGRAFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNDFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VVDPLAKDFSGFVFKIQANMDPKHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     PKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWISEDDLDQRMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     TLEEKM"
     gene            42116..43735
                     /locus_tag="VHD02_000139"
     CDS             42116..43735
                     /locus_tag="VHD02_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ5496780.1"
                     /translation="MKFTEFNLSQDIQSAVVTAGFEKASPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIRTNENIIQALVIAPTRELAVQSQEELFRFGREKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALILDHVETLILDEADEMLNMGF
                     LEDIEAIISRVPADRQTLLFSATMPAPIKQIGVKFMKDPEHVQIKNKELTNVNVDQYY
                     VRVKEQEKFDTMTRLMDVNQPELSIVFGRTKRRVDEITRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDQIDILVATDVAARGLDISGVTHVYNYDITQDPESYVHRIGRTGRAG
                     KSGESITFVSPNEMGYLSMIENLTKKQMKPLRPATAEEAFQAKKKVALKKIERDFADE
                     TIRSNFDKFKGDAVQLAAEFTPEELALYILSLTVQDPDSLPEVEIAREKPLPFKYVGG
                     GHGNKNGKGGRGRDNRNRGDRRGGYRGDRNRDERDGDRRRQKRDKRDGHDGSGNRDFK
                     RKSKRNSKDFFNKEKKSSAKNTGFVIRHKGE"
     gene            43862..45862
                     /locus_tag="VHD02_000140"
     CDS             43862..45862
                     /locus_tag="VHD02_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEQ5496781.1"
                     /translation="MSDSHLTAFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GVNQVSESFILGSISLIIWTLTLITTIKYVLIALKADNHHEGGIFSLFTLVRKMSPWL
                     IVPAMIGGATLLSDGALTPAVTVTSAIEGLKAVPGLSHIYQNQTNVIITTLVILIVLF
                     GIQRFGTGFIGKIFGPVMFIWFSFLGVSGFFNMLGHLEIFKAINPYYALHLLFSPENH
                     RGIFILGSIFLATTGAEALYSDLGHVGRGNIYVSWPFVKMCIVLSYCGQAAWILANKH
                     SGIELNPFFASVPSQLRVYLVSLATLAAIIASQALISGSFTLVSEAMRLKIFPLFRVT
                     YPGANLGQLYIPVINWILFAVTSCTVLAFRTSAHMEAAYGLAITITMLMTTILLKYYL
                     IKKGTRPILAHLVMAFFALVEFIFFLASAIKFMHGGYAVVILALAIVFVMFIWHAGTR
                     IVFKYVKSLNLNDYKEQIKQLRDDVCFDLYQTNVVYLSNRMQDHMIDRSILYSILDKR
                     PKRAQVYWFVNVQVTDEPYTAKYKVDMMGTDYMVRVNLYLGFRMPQTVPRYLRTIVQD
                     LMESGRLPKQEQEYTITPGRDVGDFRFVLIEERVSNARQLSNFERFIMQTKASIKHVT
                     ASPMRWFGLQYSEVTLEVVPLILSDVLKLPIKELVPVEDSEA"
     gene            complement(45886..46164)
                     /locus_tag="VHD02_000141"
     CDS             complement(45886..46164)
                     /locus_tag="VHD02_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEQ5496782.1"
                     /translation="MTTKKAYMYVLECVDKTLYTGYTTDLKKRLATHNAGKGAKYTRY
                     RLPVSLLYYEVFDSKEAAMSAEALFKKRKTRSQKLAYIATHQKEKKNH"
     gene            complement(46154..47008)
                     /locus_tag="VHD02_000142"
     CDS             complement(46154..47008)
                     /locus_tag="VHD02_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEQ5496783.1"
                     /translation="MKADFSKVKITLQTFTSSLFYVKITGMIKAILKEGERIDQLFSS
                     DVGIIQNKDVFSYSIDSVLLSRFPKMPSKGLIVDLCSGNGAVGLFASTRTKAAIIEVE
                     LQERLADMGQRSIQLNQLEDQVTMICDDLKNLLNHVPRSGVDLMLCNPPYFKSHESSK
                     KNVSEHYLLARHEVTTNLEEICQVARHALKSNGRLAMVHRPDRFLEIIDSLRANGLAP
                     KRVQFVYPKLGKSANMLLIEAIKDGSIEGMTILPPLVVHKENGEYTDHIFEIYFGAAS
                     KGKPNHDH"
     gene            47048..47788
                     /locus_tag="VHD02_000143"
     CDS             47048..47788
                     /locus_tag="VHD02_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEQ5496784.1"
                     /translation="MFYAYLRGLVVFLLWVVNGNAHYHHEEKMLDASENYILVAPHRT
                     FWDPVYMAFAARPKQFIFMAKKELFANRLFAWWIKMCGAFPIDRDKPSPDAIRYPVNM
                     LKKSNRSLLMFPSGSRHSQEVKGGVAVIAKLAKVKIMPAAYQGPMSVKGLLAGERVDM
                     TFGNPIDVSDIKRMNDEGIAEVANRIQAEFDRMDDELAPFQPGKARNPLTYLYRLPLG
                     LVLVVVLLLTMLFSYIASFVWNPDKHRH"
     gene            47988..48650
                     /locus_tag="VHD02_000144"
     CDS             47988..48650
                     /locus_tag="VHD02_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEQ5496785.1"
                     /translation="MLDELLIKGKSFLRERYFLPYLISLLLGLFLILSFSFLFWGNRQ
                     SKAAVPALREISPVKQQVSEEKKEIQEDSSILVDLKGAVQKEGVYKLTASSRVRDVIE
                     LAGGLTSEADKHAINFAEKLTDEQVVYVPKQGEEISVLPRSLVSGKKETASKDQSKVH
                     INKASLEELQHIPGIGAKRAQDIIDMRDKLGGFKALEDLRQVSGIGEKTLEKLKDDIF
                     LD"
     gene            48631..50874
                     /locus_tag="VHD02_000145"
     CDS             48631..50874
                     /locus_tag="VHD02_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEQ5496786.1"
                     /translation="MTFSWTKLVPLSKIQFAFLILVLFYQIHSPSWLTFLLSLSLICL
                     LVKRLSKKEFLGVFAILSFCALFLLYQKQQLIQKLEIQPVQITSVALVPDSIRINGDQ
                     LAVLGRHGKHSYQLFYRLKSQAEAQLFKKEHRWLVMHAKVTLEKAEEVRNFKGFNYQT
                     FLAYQGIYRIGKVEQIEQLEVISPESICDYLSSLRRRAIVHCQQHFPRPMSHYLTGLL
                     FGYLDKSFGEMTDYYSQLGIIHLFALSGMQVGFFLTCFRRVLLLLAVPLEWIKWIELP
                     FACFYAALTGYSISVIRSLVQSQLRHLGIKGLDNLACTFLLVFLWDAHFLMTVGGVLT
                     FSYAFLLTVVTVEELSGAKRQLVQVLTISLGILPFLLFYFSSFNPMSMVLTGLLSYLF
                     DLFILPLLCLVFCLSPLVTVSICNHLFILLEKVIQFLGNTFNSSLVFGSPTSWHLLIL
                     VISFAIFYDYRQVRQRVITCGLVIALTLLSVKYPLTNEVTFIDIGQGDSILVREWTGK
                     NLLIDVGGRPFFSSKEHWRRGHHVANAQKTLIPYLKSRGIHTIDQLLVTHADTDHMGD
                     IEVVAKAIRIKEILTSQGSLSHPSFVRRLRRLKCHVRVLAAGDQLPIMGSVLQVLYPW
                     QLGDGKNNDSLVLYGRLLNRTFLFTGDLEKEGENEIIKRYPQLRVDYLKAGHHGSNTS
                     SSAAFLDHIQPKVAFISAGKNNRYQHPHRETLARLEDRQITYYRTDTQGAIRLTGWTS
                     WHLETVR"
     gene            50945..51985
                     /locus_tag="VHD02_000146"
     CDS             50945..51985
                     /locus_tag="VHD02_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEQ5496787.1"
                     /translation="MIAIEKIEKLSKENLGLITLVTGDDIGQYSQLKSRLMEQIAFDK
                     DDLAYSYFDMSEAAYQDAEMDLVSLPFFAEQKVVIFDHLLDITTNKKSFLKEKDLKAF
                     EAYLENPLETTRLIIFAPGKLDSKRRLVKLLKRDALVLEANPLKEAELRTYFQKYSHQ
                     LGLGFESGAFEQLLLKSNDDFSQIMKNMAFLKAYKKTGNISLTDIEQAIPKSLQDNIF
                     DLTRLVLGGKVDVARDLIHDLRLSGEDDIKLIAIMLGQFRLYLQLAILAKEGKSEQQL
                     VTSLSEILDRRVNPYQVKYALKDSRTLSLAFLTGAVKTLIETDYQIKTGLYEKSYLVD
                     IALLKIMTHSQK"
     gene            52082..52687
                     /locus_tag="VHD02_000147"
     CDS             52082..52687
                     /locus_tag="VHD02_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEQ5496788.1"
                     /translation="MAIILPELPYAYDALEPQFDAETMTLHHDKHHATYVANTNAALE
                     KHPEIGENLEELLADVTKIPEDIRQALINNGGGHLNHALFWELLSPEKQDVTPDVAQA
                     IDDAFGSFDAFKEQFTAAATGRFGSGWAWLVVNKEGQLEITSTANQDTPISEGKKPIL
                     ALDVWEHAYYLNYRNVRPNYIKAFFEIINWKKVSELYQAAK"
     gene            52854..53080
                     /locus_tag="VHD02_000148"
                     /pseudo
     CDS             52854..53080
                     /locus_tag="VHD02_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922433.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            53065..53313
                     /locus_tag="VHD02_000149"
     CDS             53065..53313
                     /locus_tag="VHD02_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496789.1"
                     /translation="MDADLAQMDALGLYYDDNHLSLVNTVKVYLKEFGIGESQIAFSY
                     KDLNSGRTAAMNDHQPMIAGSTYKLPLNMLVVDKANYP"
     gene            53310..53900
                     /locus_tag="VHD02_000150"
     CDS             53310..53900
                     /locus_tag="VHD02_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEQ5496790.1"
                     /translation="MTERFDITETDYEYDQEHHAYVAQFNGAMSIPDMQEYSLVYSEN
                     TPAYALAERLGGMNKAYQLFDRYGKVSGAITTIDRNGNKITTAYYLQGLDYLWQHQDK
                     YKDILYYIGESFPDLYYKTYLPHVKVYQKPGYVREALNVGAIVCEESPYLITLYSSGL
                     GGATQASEEVNGLGYVQLVQLTYVINEWHRVNHNMI"
     gene            complement(54014..55234)
                     /locus_tag="VHD02_000151"
     CDS             complement(54014..55234)
                     /locus_tag="VHD02_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEQ5496791.1"
                     /translation="MKLLGKWLFFLMFLTFCVSFWQKDYQRIAFEKEHKLYQNLVAPF
                     YQNYPKLRELQLSEAIKLRHDLLDYVRKLDKDGWSYKAIQKGYLEQLAVRGNSYHFEQ
                     LYSRIRLIGSPDFQKLWQQEEMAQTPQEAQKRLQLLLTYLKMPDELTGQSKQTQQVLA
                     QLSPKLSPTDPFWDQLSTLIQAYYVNLEHIPYSRFNRKIHQLRYLISTQQTEWVRSHY
                     GNKGKTDADALAKYLATLEDDDYALYESSRYHNKVASILDANGNHQAVYTDNIPQANY
                     KILIHFHSEFILSESGQFLVALDPDNLTRNSIVNGSSFNYGNQNDDLHRLLDIDPILL
                     FDPAFIEKAINSPDATFLVPDLEQQRDKHNPIYSRNGKSSKQLTRAAVKKFKKLLHHY
                     QETRGDNKTSKTQH"
     gene            complement(55241..56269)
                     /gene="queA"
                     /locus_tag="VHD02_000152"
     CDS             complement(55241..56269)
                     /gene="queA"
                     /locus_tag="VHD02_000152"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEQ5496792.1"
                     /translation="MNTNDFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFD
                     HIIDQLNPGDALVMNNTRVLPARLYGEKPDTHGHVELLLLKNTQGDQWEVLAKPAKRL
                     KVGSQVNFGDGRLKATIIDELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     AERYQTVYAKENGSAAAPTAGLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHDMHSEFYSLSEEAAQTLRDVKQAGGRVVAVGTTSIRTLETIGGKFQGDIQADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLEAYRHAVDEKYRFFS
                     FGDAMFVN"
     gene            56471..57175
                     /gene="nagB"
                     /locus_tag="VHD02_000153"
     CDS             56471..57175
                     /gene="nagB"
                     /locus_tag="VHD02_000153"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEQ5496793.1"
                     /translation="MKIIRVQDQIEGGKIAFTLLKDSLAKGAKTLGLATGSSPISFYQ
                     EMVKSPLDFSDLTSINLDEYVGLSVESDQSYDYFMRQNLFNAKPFKKNYLPNGLATDI
                     EAEAKRYDQIIAGHPIDFQVLGIGRNGHIGFNEPGTSFEEETHVVDLQESTIKANSRF
                     FTSIEDVPKQAISMGIASIMKSKMIVLLAFGQEKADAIKGMVFGPITEDLPASILQKH
                     DHVIVIVDEAAASQLD"
     gene            57294..58010
                     /locus_tag="VHD02_000154"
     CDS             57294..58010
                     /locus_tag="VHD02_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEQ5496794.1"
                     /translation="MRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPG
                     LQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNYLTVIDQLAEEDKSPDLYPVGRL
                     DRDTEGLVLLTDNGPLGFRMLHPIHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQ
                     CQPAQLTILKADTDQSQASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLA
                     SGEYRYLTASERQLIKTYLD"
     gene            58305..59267
                     /locus_tag="VHD02_000155"
     CDS             58305..59267
                     /locus_tag="VHD02_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEQ5496795.1"
                     /translation="MTKILTALDGKNQLISLVTQPISTKPPFCCPACKSPVRLRQGTI
                     RRPHFAHVQLAHCQFQAENESEEHLTLKAKLYTSLVRTEAVCIEKYLPELQQIADLWV
                     NDKLALEIQCSPLPVERLKKRTKAYQEKGYPVRWLLGRKLWLNTHLTALQKQFLYFSS
                     SLGFHLWELDAAANLLRLKYLIHEDLFGKVSYLTKTISLDHNIMEMFRLPYQQEILYS
                     YQKKMTVNLSKRIQRALLARHPKWLRRQEKAYLSGYNLLMLTTDAFYPQWRPVQSSSG
                     FCQIEGNLRPYYESFKVYYKKEKDKKVQTLFSPKYYVKMDSNRK"
     gene            59280..61085
                     /gene="pepF"
                     /locus_tag="VHD02_000156"
     CDS             59280..61085
                     /gene="pepF"
                     /locus_tag="VHD02_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEQ5496796.1"
                     /translation="MTDNRSHLEEKYTWDLSTIFATDKDWEAEVSDLATEIDAAKDFS
                     GHLLDSSANLLTITDTYLELARRVEKVYVYAHMKNDQDTTVAKYQEYQAKASGLYAKF
                     SEVFSFYDPEVMALSQEDYQAFLAETPDLKRYHHFFDKLFQTRDHVLSQAEEELLAGA
                     QEIFNGAEETFSILDNADIVFPVVKNDKGEDVELTHGNFISLMESKDRSVRQAAYEAM
                     YSTYEQFQHTYAKTLQTNVKVQNYKARVHKYDSARQAAMAANFIPEAVYDTLLETVNK
                     HLPLLHRYLKLRQEVLGLDDLKMYDVYTPLSETDLAIGYDEALEKAEKVLAVFGKDYA
                     DRVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRETQPYVYGDYSIFLAEIASTTNENIMTEALLNEVQDEKERFAILNHYLDGFR
                     GTVFRQTQFAEFEHAIHQADQKGEVLTSEYLNQLYADLNEKYYGLSKKDNHFIQYEWA
                     RIPHFYYNYYVYQYATGFAAASYLADKIVHGTQDDIDHYLAYLKSGNSDYPLEVIAKA
                     GVDMTKGDYLEAAFKVFDERLTELEVLVSKGIHKS"
     gene            61461..62657
                     /locus_tag="VHD02_000157"
     CDS             61461..62657
                     /locus_tag="VHD02_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEQ5496797.1"
                     /translation="MEKTKRYIIATAGILLHLMLGSTYAWSVYRNPILQETGWDQAPV
                     AFAFSLAIFCLGLSAAFMGNLVEQYGPRLTGTVSAILYASGNMLTGLAIDRKEIWLLY
                     IGYGVIGGLGLGAGYITPISTIIKWFPDKRGMATGFAIMGFGFASLLTSPIAQWLIET
                     EGLVATFYLLGLIYLIVMLFASQLIIKPTAAEIAILDKKRLQNNSYLIEGMTAKEALK
                     TKSFYCLWVILFINITCGLGLISVVAPMAQDLTGMSPEMSAIVVGAMGIFNGFGRLVW
                     ASLSDYIGRRVTVILLFLVSIIMTISLIFAHSSLIFMISIATLMTCYGAGFSLIPPYL
                     SDLFGAKELATLHGYILTAWAIAALTGPMLLSITVEWTHNYLLTLCVFIVLYILGLMV
                     VLRLKK"
     gene            62723..63430
                     /locus_tag="VHD02_000158"
     CDS             62723..63430
                     /locus_tag="VHD02_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEQ5496798.1"
                     /translation="MVKSYSKTANHNMRRPVVKEELVHYMRTRQKQTTGFLAELEQFA
                     RQENIPIIQPEVVAYFRFLLQSLQPKHILEIGTAIGFSALLMAENAPDATIVTIDRNR
                     EMIDFAKANFAKYDSRQQIRLLEGDAADILSTLEGNFDFVFMDSAKSKYIVFLPEILR
                     LLKVGGVVILDDVFQGGDITKPIEDIRRGQRTIYRGLQSLFDATLTHPNLTTSLVPLS
                     DGLLMIRKNQADIVLPD"
     gene            63493..64548
                     /gene="prsA"
                     /locus_tag="VHD02_000159"
     CDS             63493..64548
                     /gene="prsA"
                     /locus_tag="VHD02_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEQ5496799.1"
                     /translation="MKNSNKLIASVVTLASVMALAACQSTNDNTKVISMKGDTISVSD
                     FYNETKNTEVSQKAMLNLVISRVFEAQYGDKVSKKEVEKAYHKTAEQYGASFSAALAQ
                     SSLTPETFKRQIRSSKLVEYAVKEAAKKELTTQEYKKAYESYTPTMAVEMITLDNEET
                     AKSVLEELKAEGADFTAIAKEKTTTPEKKVTYKFDSGATNVPTDVVKAASSLNEGGIS
                     DVISVLDPTSYQKKFYIVKVTKKAEKKSDWQEYKKRLKAIIIAEKSKDMNFQNKVIAN
                     ALDKANVKIKDKAFANILAQYANLGQKTKAASESSTTSESSKAAEENPSESEQTQTSS
                     AEEPTETEAQTQEPAAQ"
     gene            64935..67553
                     /gene="alaS"
                     /locus_tag="VHD02_000160"
     CDS             64935..67553
                     /gene="alaS"
                     /locus_tag="VHD02_000160"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEQ5496800.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPDWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYNPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKHDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDAAAKVQALS
                     DSLRDLQKENAELKEKAAAAAAGDVFKDVQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAETV"
     gene            67913..68128
                     /locus_tag="VHD02_000161"
     CDS             67913..68128
                     /locus_tag="VHD02_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ5496801.1"
                     /translation="MEVILKNRLKELRARDGINQTEMAKLAGVSRQTISLIERNEYIP
                     SVIIAMKIAKVFQEPVEEVFRLVEVEE"
     gene            68125..68886
                     /locus_tag="VHD02_000162"
     CDS             68125..68886
                     /locus_tag="VHD02_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEQ5496802.1"
                     /translation="MKKKKNSFLRLLKMLLLSSLAGGIIGGMVGAFLGYHGERLDQLT
                     FLKDDVINLIILLNRLVVVTGLTLSFVFLTQLKKETAVYNTIEEDDYSENGYRQLNKK
                     HAYTMLLIAVASILSMCNVLLGLTLTNDSQHAMLAIPLLDILLLLMVIPFQALAMKRY
                     NAIQGTDVPYFPNLKELKHNIMALDEAELQAYHKTSFESVLSLNGVIIPSLYVILFFV
                     YLFTGQVELTAILVLVLIQLYLLVKSATMTRQFYR"
     gene            68905..69600
                     /locus_tag="VHD02_000163"
     CDS             68905..69600
                     /locus_tag="VHD02_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEQ5496803.1"
                     /translation="MKGFINYLKIAVLIILAMVFNVLPMILLQKQHDIPMVLNWGIGI
                     FYLVIVGSVLIVLWRLYQAKQDTPIKQQKMRLVDWGYLALFWLIIRVIAIAGTLVNQL
                     WSGQQVSANDAAIHTLARLIKGGFPLYTALFVLVIAFIAPIMEELVFRGFPMIDLFKG
                     KSLKVAGLVTSLVFALPHATNSVEFIMYSCMGIFLFVAYQRRGNLKDAILLHIFNNLI
                     EVILLMSIGLGVI"
BASE COUNT        21591 a        12004 c        14617 g        21500 t
ORIGIN      
        1 tctgtttttt agcagttttt agaatagcaa aagtttggaa agtaagtgag gtcaagtgaa
       61 tggctagaaa tggctttttt actggtttgg acattggaac aagctcgata aaagttttag
      121 tagcagaatt tatttctggt gagatgaacg tcattggtgt tagtaatgtt ccaagtaccg
      181 gcgtaaaaga tggcataata atcgatatag aggcagctgc gactgccatc aaaactgcgg
      241 tagaacaagc agaagaaaaa gcagggatga caattgaaaa ggttaatgtt gggctaccgg
      301 caaaccttct tcaaattgaa ccaacacaag gaatgattcc tgtcccaagt gagtctaaag
      361 agataaaaga tgaggatgtt gatagcgttg ttaaatcggc tttaacaaaa agtatcacac
      421 cagaacgaga ggttatctct ttagttccag aagagttcat tgtggatggc tttcagggca
      481 ttcgagatcc acgtggtatg atggggatta gattagagat gcgcgggctt atttatactg
      541 gaccaagcac cattttacat aatctgcgta aaacggtaga aagagcaggc attaaagttg
      601 aaaacatcat tatttctccg ttagctatgg ctaaaaccat tttaaacgaa ggtgagcgcg
      661 agtttggagc tactgtaatt gatatgggag gtggacagac aactgtcgct tctatgcgag
      721 cacaagaatt gcagtatacc aatatatatg ctgaaggcgg cgaatacatt actaaagata
      781 tatcaaaagt attaaaaacg tctttggcta ttgcagaagc acttaagttt aattttggtc
      841 aagcggagat atcagaagct agtataactg aaacagtaaa agttgatgtg gtaggtagtg
      901 aagagcctgt tgaggtaact gaacgttatt tatctgaaat tatttcagcg cgtattcgtc
      961 atattttaga tcgtgtgaag caagatttgg aaagaggtcg tttactagac ttaccaggag
     1021 gcattgtttt gattggtggc ggtgcaatca tgcctggagt ggtagaaatt gcacaagaaa
     1081 tctttggagt aactgtaaag ctccatgttc caaatcaagt cggtattaga aatccaatgt
     1141 tttcaaacgt tatcagtttg gtagaatatg ttggtatgat gtctgaagta gacgttttag
     1201 cacaaactgc agtttcagga gaagaacttt tgcgacgcaa gcctattgat ttcagtggtc
     1261 aagaatctta tttaccagat tatgatgatt caagaagacc agaatcgacc attggctatg
     1321 aacaacaagc gtcacaaaca gcatatgatt cacaagttcc gagtgatcct aaacaaaaaa
     1381 tttcagaacg tgttcgtggc atatttggga gtatgtttga ttaaaagtaa taaagtgagg
     1441 aaataaaatg gcattttcat ttgatactgc atcaattcaa ggtgcaatta taaaagtaat
     1501 tggagtcggc ggaggtggcg gaaatgccat taatcgtatg attgatgaag gtgttgctgg
     1561 tgttgagttc atcgcagcaa atacagacat tcaggcatta agctcatcaa aagctgaaac
     1621 ggttattcaa ctaggcccta aattaactcg tggacttggt gctggaggac aacctgaagt
     1681 aggacgtaaa gctgctgaag aaagcgaaga aattttaaca gaagctctta caggagcgga
     1741 catggtattt attactgccg gtatgggtgg tggctctggg acaggggctg caccggttat
     1801 tgctcgtatc gctaaaagtt tgggagcctt gacagtagct gttgttactc gcccgtttgg
     1861 ttttgaaggt aacaaacgtg gtaattttgc tattgaaggt atcgaagaac tacgtgaaca
     1921 agttgatact ttgttaatta tttcaaataa taaccttctt gagattgttg ataaaaagac
     1981 gcctttatta gaagcactta gtgaagctga taatgtttta cgtcagggag ttcaagggat
     2041 aaccgactta attactagtc ctggccttat caatctcgat tttgccgacg tgaaaacagt
     2101 tatggcaaat aaagggaatg ccttgatggg gattgggatt ggttctggag aagagcgcat
     2161 tgttgaggcg gcgcgtaagg caatctattc acccctatta gaaacgacta ttgatggtgc
     2221 acaagacgtt attgtaaacg ttacaggagg tctcgacatg acacttacag aagctgaaga
     2281 agcctctgaa attgttgggc aagctgctgg tcaaggcgtt aacatttggt taggaacatc
     2341 tattgatgat actatgaaag atgacatccg tgtgactgtt gtagcaactg gagtgcgcca
     2401 agaaaaagcc gagcaagttt caggttttcg tcagcctagg acttttaccc aaaccaacgc
     2461 gcagcaagta gcgggtgcac aatatgcatc agatcaagca aaacagtcgg ttcaaccagg
     2521 gtttgatcgt cgctcaaatt ttgattttga catgggggag tctcgcgaga taccaagtgc
     2581 acaaaaggta atttctaatc ataatcaaaa tcaaggttca gcttttggaa attgggattt
     2641 gagacgtgat aatatttctc gtccaacaga aggtgaattg gataaccatc ttaatatgtc
     2701 aacgttctca gctaacgatg acagtgatga tgaattagaa acgcctccat tctttaaaaa
     2761 ccgttaataa tggatttact gacaaataaa aagaagattt ttgagactat ccgcttatct
     2821 acagaggcag caaataggac taatgatagt gtttcagtta ttgctgtaac aaaatatgtg
     2881 gatagtacaa ttgcaggtca gcttatcgaa gcaggaattg agcacattgc cgaaaaccgt
     2941 gttgataaat ttcttgaaaa gtatgatgcg ttaaagtgta tgccagtaaa gtggcatttg
     3001 atcggtacct tacaacgtcg taaggttaag gaagttatca attatgttga ttattttcac
     3061 gctctagatt ctgtgagatt agctttggaa atcaacaaga gagctgacca tcctgtgaag
     3121 tgttttctac aagttaatat ttctaaagaa gagagtaaac atggttttaa catttctgag
     3181 attgatgaag cgattgaaga aataggtaag atggagaaga tacagttagt tggtttaatg
     3241 actatggcac cagcaaatgc cagtaaagaa agtattataa ctatttttcg acaagcaaat
     3301 caattaagaa aaaacttgca gttaaaaaaa agaaagaata tgccttttac agaattgagc
     3361 atgggcatga gtaacgatta tccaattgct attcaagaag gctcaacttt tattcggatt
     3421 ggtagagctt tctttcacta atggagaata agatggcttt taaagataca tttaacaaga
     3481 tgatttctta ttttgacacg gatgaggtta acgaagttga agaagatgtt gcagcatcaa
     3541 ctgataacgt tattccaaga tcacaacaat cagtcagagc aagtagtcat ccaaaacaag
     3601 aacctagaaa caatcacgta caacaagatc atcaagcgag atcccaagaa cagacaaggt
     3661 cacaaatgca tccaaaacat ggtacttctg aacgctatta tcagcagtct cagccaaaag
     3721 aaggccatga aatggttgac agaagaaaac ggatgaccac ttctggtatt gcaaatcgcc
     3781 gtgagcagta tcaacaatca acttgttcag atcaaacaac tattgcctta aagtatcctc
     3841 gtaaatatga ggatgctcaa gaaattgtgg atcttttaat agttaatgaa tgcgttttga
     3901 ttgattttca gtttatgcta gatgctcagg ctagacggtg tttagatttt attgatggtg
     3961 ctagtaaagt gctctatggt agcttacaaa aggtaggctc ttcaatgtac ttactggctc
     4021 cgtcaaatgt atccgtcaat atagaagaaa tgactatccc acatactaca caagatattg
     4081 gctttgattt tgatatgaaa aggcggtaaa taaatgatat taatactatc tattcttctt
     4141 cgtctgatca aagtctacac ttatttattg attgcttacg cactaatgtc atggtttcct
     4201 ggggcatatg attcaaaaat tgggcgtttg attagtggta tcgttgaacc aattttaaaa
     4261 ccttttagag catttaattt acaatttgcc ggtcttgact tcactatttt tgtcgtcatt
     4321 attagtttga attttttagc tcaagttttg gtccgtgtgt ttatttaatg attagtcata
     4381 gtaagattta tcagcatttt caccaagaag aatatccttt tattgataga atgtctgata
     4441 tgattaatag agttgaagat tactatcttt tagaagttac tgagttttta aatcctagag
     4501 aagtgatgat tttaaaaagt ttgattgctt taacagatct aaaaatgttc gtatcaacag
     4561 attactatcc aagcgaatat ggtcgtgtta ttattgcacc tggttactat gacttagaac
     4621 aaagtgattt tcaaatagct ttagtagaga taagttatca ggcaaagttt aatcagttga
     4681 cacatagtca aattttagga actttaatta atgaattagg agtaaagcga aatttatttg
     4741 gagatgtttt tgttgaaatg ggatatgccc agctcatgat taagcgggag ttattggatt
     4801 attttttagg aacaattact aaaatagcta aaactagtgt gaaattaaga gaagttaact
     4861 ttgatcagtt aattaggtct attgataaca gccagaccct ggatattcta gtttctagtt
     4921 ttcgattaga tggtgtagtt gctactatct taaaaaaatc tcgaacgcaa gttatagcat
     4981 taattgaagc aaataagatt aaggtaaact atcgactcgc taataaagct tcagataatc
     5041 tagtcatagg ggatatggtg agtatcagag gtcacgggcg ttttactctt ttagcagata
     5101 atggagtgac caaacatggc aaacaaaaaa taacactaag taaaatgata cataaataag
     5161 gagatacaat ggcacttaca acgctagaaa ttaaagataa aacttttaag acaaaattcc
     5221 gaggatactg tgaagaagaa gtcaatgaat tccttgatat tgttgttgat gattacgaag
     5281 ctcttgtacg taaaaatcgt gataacgaag caagaattaa agatcttgaa gaaaaattat
     5341 cttactttga tgaaatgaaa gagtcactta gccagtctgt aatcttagct caagaaacag
     5401 ctgaaaaagt gaaagcaacg gctaatgcgg aagcaacaaa cttggttagt aaagcgactt
     5461 atgatgctca gcatttatta gatgaatcta aagctaaagc caatcaaatg ttgcgtgatg
     5521 caactgatga ggctaagcga gttgcgattg aaacagaaga attaaagcgt caaacacgtg
     5581 tgttccacca acgtttaatc tcatctattg agtcacaatt aagcttatca aattcacctg
     5641 aatgggatga actgttacaa ccgactgcaa tctatcttca aaattctgac gatgctttta
     5701 aggaagtcgt gaagaccgtt cttaatgaag acattcctga atctgatgat agtgcctctt
     5761 ttgatgcaac acgtcagttt actccagaag agttagaaga attgcaacgt cgtgttgatg
     5821 aaagcaataa ggagttagag gcttaccaac ttgactctca atctgattct acgactgagc
     5881 cagaggtaaa tctcagtgaa acacaaacgt ttaaattaaa tatctaaata aagaaaaaca
     5941 agatgatttg cttataatca cagcaagcgg gagatgatga gagtctcgta ttccctaagt
     6001 attatcatta tcattttcta aaattttcgc tgaactacag taagcgagga ggtttgttca
     6061 ccttacgagc aaagagggag cggtaagtct tatgatatat tatcgctctg aactaaggtg
     6121 gtaccacgag ctttcgtcct ttttgagacg aggctttttt cgttatagtc gcaagtttgt
     6181 ttaaaaggag tttgaaataa tgaaattaaa agagacactt aatctaggaa aaacagcctt
     6241 tccaatgcgc gcaggtcttc ctaataaaga gccacaatgg caagcagctt gggaacaagc
     6301 agagctttac aaaaaacgtc aagaattgaa tgcaggtaag cctgcctttc atcttcatga
     6361 tggacctcca tacgctaacg gcaatattca cgttgggcat gcccttaata aaatttcaaa
     6421 agatatcatt gtgcgttcta agtccatgtc tggctttcaa gcgccttatg tacctggttg
     6481 ggacacacac ggccttccaa tcgaacaagt cttggctaag caaggaatca aacgtaagga
     6541 gatggattta gcagaatacc ttgagatgtg tcgtcaatac gctttaagtc aggttgataa
     6601 acagcgagat gatttcaaac ggttaggtgt ttcggcagac tgggaaaatc cttatgtcac
     6661 tttggaccca cagtttgaag ctgatcaaat tcgtgttttt ggagcgatgg ctgaaaaagg
     6721 ttacatctat cgtggtgcta aacctgttta ttggtcatgg tcttcagaat cggccttggc
     6781 agaagctgaa attgaatacc atgacattga ttcaacctct ttatactacg ccaataaagt
     6841 taaagatggc aaaggcatcc ttgatactaa tacgtatatc gttgtttgga caacaacacc
     6901 atttacggtt acagcatcac gtggtttgac tgttgggcct gatatggatt atcttgtggt
     6961 gaaacctgct ggttcagacc gtcaatatgt tgtggcagaa ggtcttttgg atagccttgc
     7021 tggaaaattt ggctgggaat cttttgaaac tttagccagc cataaaggag ctgatttaga
     7081 gtacattgtg acagaacacc catgggatac tgacgtagaa gaattggtta tccttggtga
     7141 ccatgttacc cttgagtcag ggacagggat tgtccacaca gcaccaggtt ttggtgagga
     7201 tgactacaat gttgggacaa aatacaaact ggaagttgct gtgactgttg atgaacgtgg
     7261 cctaatgatg gaaaatgcag gtccagattt ccacggacaa ttttataaca aggtaacgcc
     7321 gattgtcatt gataaacttg gtgatctttt attggcacaa gaagtgatca atcactctta
     7381 cccatttgac tggcggacga aaaaaccaat catctggcgt gctgtaccgc aatggtttgc
     7441 ttctgtttct gatttccgtc aagatatttt ggatgaaatt gaaaaaacaa ccttccatcc
     7501 atcatggggt gaaacacgcc tttacaatat gattcgtgat cgtggcgact gggttatttc
     7561 tcgtcaacgt gcttggggag ttccgcttcc tatcttctat gccgaagatg gcacagctat
     7621 tatgactaaa gaagtgacag atcatgtggc tgatttgttc caagaaaatg gatcaatcat
     7681 ttggtggcaa aaagaagcta aagacctatt accagagggc ttcactcatc cagggtcacc
     7741 aaatggcgag tttacaaaag aaactgatat catggacgtt tggtttgact ctggttcttc
     7801 ttggaatgga gtcatgaatg ctagagaaaa tctttcctat ccagcggatc tctacttaga
     7861 aggctctgac cagtatcgtg gttggttcaa ttcatcctta attacatctg tggctgttaa
     7921 tgggcatgct ccatataagg ctattttatc gcaaggcttt gttcttgatg gtaagggcga
     7981 aaaaatgtct aaatcaaaag gcaatatcat ttctccaaat gatgtggcta aacaatatgg
     8041 tgcagatatt ctccgtctct gggtagcttc tgtggatacc gataatgata ttcgtgtttc
     8101 tatggagatc cttggtcaag tctctgaaac ttaccgtaaa atccgtaaca cccttcgctt
     8161 cttgattgct aatacatctg atttcaaccc tgctacggat acagtagctt atgcagactt
     8221 aggagctgtt gataagtaca tgacaattgt ctttaatcag ttggtagcaa cgattactga
     8281 cgcttatgag cgttatgact ttatggctat ttacaaagca gtggtgaact ttgttacggt
     8341 tgatttatca gccttctatc ttgattttgc taaagacgtt gtctatattg aagctgccaa
     8401 tagcttagaa cgtcgtcgca tgcagacagt cttctatgat atcttggtta agattaccaa
     8461 gttattgacg ccaatcttgc ctcacaccac tgaagaaatc tggtcttatc tagaatatga
     8521 gtcagaagca tttgttcaat tggcagaaat gcctgtggca gaaactttct cagctcaaga
     8581 ggatatttta gaagcttggt ctgctttcat gactttgcgt acccaagctc aaaaagcttt
     8641 ggaagaagcg cgaaatgcta agattatcgg taaatcattg gaagcccatt tgaccatcta
     8701 cgctagtgaa gaagtgaaaa ccttattgac tgctttagac agcgatattg ctttgctttt
     8761 gatcgtgtct caattaacca ttgctgactt ggcagatgcg cctgcggatg cagtggcatt
     8821 tgaaggtgtt gcctttatgg tagaacatgc cataggtgaa gtttgtgagc gttcacgccg
     8881 catcgaccca actactcgca tgcgttctta caatgcattt gtctgtgatc acagcgctaa
     8941 aatcattgaa gaaaatttcc cagaagctgt ggctgaaggg tttgaagaga gtggcaaata
     9001 agtcttatca agacttgaag tgacactgag taagaagaca accatcgggt cttaggacct
     9061 gatggttttt tagtagagct gattacgtcc attagccagc tttaacgaga gacaaagtag
     9121 gagaaattta gactactttt tttatttctt ttaagaaaat tgctggcttc atataaggac
     9181 tattaagata gagttcacct ttcgctaata aaaaacttag ctcttttaga agggctaagt
     9241 tttgtctgaa tagctggctg ctgtttatac agaagtttga aatgaaattt caataaggtt
     9301 attcgtgtga agtgtttttg attgtccagg aataacaatt ttaggatcaa ttttacgttt
     9361 taaaaaaaat tcgcgaatga tatcaagttc atcctccttg atggcacggt atttatttga
     9421 aataagtagt tcgtaatgtt ttggtgcttg ggtaaaaatg acatctgtat ttcctgctga
     9481 gtatacttcg actaaatagg catcggtgtt ttttagctga tttctgatga gttctgggtg
     9541 actgctagtg gtattgatga gtctcataaa tgtcctcctt agtattcctg tacctattat
     9601 agcactaatt taaagcctta gttagtagaa tgatgctttt tttgccattc cttgactccc
     9661 cattgatggc tctcccgttt taatttagca attgcctcta taggagaaaa ccaacctaag
     9721 ttattaaaat cttctaaggg cttagaaaca gcttgaaatg cagttacttc ataaagatag
     9781 gcaggatggt agaagtgggt atcacgatgg cgagagtaga aatattcatc agcctgaccg
     9841 taatatgaac caatagtagc agaaaaacca agttcttcaa tcagttcacg ctctagggcc
     9901 tgaagctgac cttcacctgc ttcaatttca ccaccaggta aaaaccagga accattagga
     9961 gcttgaacaa gaataatttt tgtttgttcg tgattgggaa tgatagcata gacaccgtaa
    10021 cgtgtgacat agtctttatg tgcattttta tgtccaaatg ttggaatcat cattttattt
    10081 acctttcctt tttcttcatt atactaaatt tttgctaatt attcttatca atttgtttag
    10141 tttaataaaa aattgataga aaaagagaga gtctccttat agaaactctc ttagctaacg
    10201 ttaatctaga gactcttcgt taagaaaaga atctccctct ttaacagctt taatcgtgat
    10261 gttttcactg tttgtcatca gtgcacgtaa ctgtttttca gttgggtgtt ctaaataata
    10321 gtctgtaatg gcatcttcga tataatcttg gatagtacga cggagcggcc gagcgcccat
    10381 tttaggatca tagcctaaat ctactaattt ttctttgacc ttttgtgtca catcaagatg
    10441 aatcccatta tagcctaagc gctcattaac atcttccagc attaaatcaa cgatatgcag
    10501 caaatgttct ttagataaag ctttgaattc aataatacca tcgaaacgat tcataaactc
    10561 tgggctgaag aagttgctta attcaccaag aacagaactt gtccgtcctt ctctagcagc
    10621 accaaatccg acagaagctt cgcttttacc tgttccggca ttagaggtca tgataataat
    10681 ggtgtccttg aagctgactg ttcgcccttg accgtctgtt aagcgaccgt catcaagaac
    10741 ttgtaagaac atgtgcatga cgtcaggatg ggctttttcc acctcatcta agagaataag
    10801 ggaataagga ttgcgacgaa cttgttcggt tagctgtcca gcttcttcat agccgacata
    10861 acctggagga gccccgacta atttagcaac ggcatgtttt tccatgtatt cggacatatc
    10921 aaagcgaatc atattgtcag cagagccaaa gagttcaata gctagttgct tagacaactc
    10981 tgttttacca acacctgttg gcccaacgaa taggaaagaa ccaataggac gatttggagt
    11041 tcccaatccc acacggttcc ggcgaatggc cttggcaatt ttgtcaacgg catcatcttg
    11101 gccaatcacg tgagctttca aatcattagc caaattaacg agttgagatt gttctttttc
    11161 tttcaagtcg ccaactggaa tattggtttt ttgttcaatg atggcttcaa tggttttttc
    11221 agtaatgata ggaatatctt gctcatcgac tttttgagcc tgcatttcct tgtatttcgc
    11281 aatttgatcg cggaaataag ctgcgcgttc atagtcttcg tctcgagtag cttgcgcctt
    11341 gagattctca gcttcaataa gacgtttgtc aatttcttta ggatcaacaa agttgagggt
    11401 tagattcatt ttggaaccag cttcgtccag aaggtcaata gccttatcag gaagaaaacg
    11461 gtcttgaata tagcggttag ataaatgagc ggcagcttca atagcggctg ggctatattt
    11521 gacatgatga tagtcttcgt atttcggttg gatacctttt aaaatcgtga tggtttcctc
    11581 aacagaaggt tcatcaactt taacgggttg catacgtcgc tccaaggcag catctttttc
    11641 aataatacgg tattcattta aggtagtagc accaacgagt tgcaactcac cacgggccaa
    11701 ggctggtttt aaaatattac cagcatccat attgccgtct cctgcagaac cagcaccgac
    11761 aatctcatga atttcatcaa taaagagaat cacatccttg cgattgcgaa tttcttccat
    11821 taatttttgc atgcgctctt caaactgacc acggatacct gttccctgaa cgaggctaac
    11881 cacatcaaga cgaatcactt gcttgccttg gagtttttga ggaacagtac catcaataat
    11941 tttttgagcc aaaccttcta caacagcagt tttaccaact ccaggctcac caattagcac
    12001 aggattattt ttagtacggc ggttgaggat ctcgataacg cgtgtaatct cttcgtcacg
    12061 accaataaca ggatcaatat taccatttct tgcaatatct gtgacattaa tcccaaactc
    12121 ttctaacaag ccctttgctt gctgatttgg tgtttgtggc tgagcaggtc gttgtccgtt
    12181 gtagttacca ccatagcgtc ctccgccatt tccattttgc cctgcctgag taggtggggt
    12241 atttggaaga ttaccaaaag ctctaaaatt attcaaatca ccaaaaaagt catcaaagaa
    12301 aggacttgtg gatctatctt gtgctctata tcctggggtt aggccattta aaatagaatt
    12361 ggcaggatcg gatttcatga tttgataaca attttgacag agatcaacct gtctttgttt
    12421 tccattaaca cttgtataaa gatgaatagt agattcgttt aaattacaat tttgacacag
    12481 cataatatac ctccgagggt ttatctaaaa gtcaaagtca tttagtcaaa aatggtcaaa
    12541 ctttatattc atattatagc accacggctt gggaaatcaa gtaaaaagtt tatcgcttta
    12601 gcgttagctt ttagagagta acatgacaga gtaactttat taggtttcca atgagcaaat
    12661 gtttaaaatc attgaaaaga gtaaaaaaag taaaatatca ataaatatat tactttcctt
    12721 gttagtttcc cttgttttgt gttaaaataa gagggataaa aaacgaatag gagaaacgaa
    12781 atggaatcac atttggtgag aatcatcaat cgtttggagt tgatggtgac agatggcggt
    12841 aatctcaagc gaaattttga acgcgatggt gttgttgttg cagaagttgc cttttcaaat
    12901 gatcctgaaa atggacctgt tttcacattg cgtgatgttg aggcgcgtga gtcatattcc
    12961 tttgatagca ttgacttgat tgcaatggaa atctacgatt tattatatta agcttgacat
    13021 agtaaagaga cttgcctgag gcaggttttt ttattgtcaa aaagtattgt attaatattc
    13081 atcagatggt ataattatct ttattgtaat gaacctagga cttatgagga gatataaatg
    13141 gatttgtcat ttttgcccaa atactgggcc tactttaact acggtgtact tgtcaccatt
    13201 atgatttcag tcagcgttgt cttttttgga acccttattg gtgtcttggt aaccctgatt
    13261 aagcgtagtc atgtgaagcc gttgacctgg gtcgttaatc tttacgtttg gatctttcgg
    13321 ggaacaccta tggtggttca aatcatgatt gcctttgctt ggatgcattt taacaatatg
    13381 cctactattg gttttggggt tttagacttg gacttttcaa gattacttcc tggaattatt
    13441 atcatttcat tgaatagcgg tgcttatatt tcagaaattg ttagagcagg tattgaggct
    13501 gtaccaaaag ggcaattaga agcagcttat tcactaggta ttcgtcctca aaatgccatg
    13561 cgttatgtga ttttgcctca ggcctttaaa aatattttgc cagccttagg aaatgaattt
    13621 attaccatta ttaaggatag tgctctttta caaaccattg gagtgatgga actttggaat
    13681 ggtgcccaat cggtggtaac ggctacttat tctccaattt cccctttact ggtggctgct
    13741 ttttactact taatggtcac aacagtgatg gcacagttat tggcagtctt agaacgtcac
    13801 atggcgcaag gaggtaatca ttgatgactg aggctattat ttcaattaaa gacttgcaca
    13861 agtattatgg gcacaatgaa gtgcttaagg gaattgattt agacattatg ccaggagagg
    13921 ttgtggtgat tattggccct tctggttctg gaaaatcgac cttacttcga actatgaatc
    13981 ttttggaagt accaactaag ggacagatta ggtttgaggg gattgatatt accgataaga
    14041 agaatgatat tttcagcatg cgtgaaaaaa tgggaatggt tttccagcag tttaacctct
    14101 ttcccaatat gactatttta gaaaatatca ctttatcgcc aatcaaaact aagggaatgg
    14161 ctaaagcaga ggctgacaaa acagccttga gcttgttgga caaagttgga ttatcagaaa
    14221 aagccaaggc ttatcctgct agtctttctg gtgggcaaca gcagcggatt gcgattgcgc
    14281 gtggactggc tatggatcca gatgttttac tctttgatga accaacttca gctctagacc
    14341 cagaaatggt gggtgaggtc ttggctgtca tgcaagattt ggctaaatct gggatgacta
    14401 tggttattgt gactcatgaa atgggctttg ctaaagaagt cgcagaccgt gtgattttta
    14461 tggatggcgg cgttattgtg gaagaaggaa gtcctaacca actttttgat ctgactaaag
    14521 aagaacgtac aaaagatttt ttgagtagag tattataaat ctgttctatg aattataaaa
    14581 ggaagtccta aatgatttag gacttccttt tataatttgc taaggcattt tataggagat
    14641 tacttttctt tttgttaaaa taaaaagaaa tagttgcatt atttgcatag aagaaaggga
    14701 tctgttatgg tttcagtgat gtggtttcgt agagatttaa ggattgatga tcaaaaagct
    14761 ttagctcatg ctattgctag tcaatcacca accttatgtg tgtttcaatt taacaaggag
    14821 caattaaatc tgctacatag tcggaatcaa tctagtttta ttactagcgt tttggctttt
    14881 agaaagttat taaaatgtga aggtattgac ttatatctaa tgtatggaga cttaatgacc
    14941 tgttttgagc aattattgac acaattaaaa gactggacag atgtgtattt taactatgat
    15001 gaatctggat atggtcgttt acgtgatcaa aaagcagcac aatttttcaa aaaaaatggg
    15061 attgccgttc atacctacca agatcactat ttacatggta gtcaagagat cattaaccaa
    15121 tcaggtcagc cttataaagt ttttacgccc tactatagga tttggcaaaa ttatccgaaa
    15181 gagactccta ttaaagtaga actatcgcaa ggcaggtggt taaatcttga gacaccagat
    15241 gatgttttaa ggactgttga gagctttaaa gatgaaaaat atcaggatgt tgcaactttt
    15301 gatgaagcgt caaaacaact aaatcgattt attcaagacc aattagcagc ttatcacgct
    15361 aatcgagatt ttccagcgca gctagggacg agtcggttgt ctcctttttt aagaatagga
    15421 gctattggca ttcgcacggt atatcacgct gtgcgtcaag caccaaatag tctagggcaa
    15481 gctacttttt taaaagaatt agcttggaga gacttttaca atatggtcta tgtggcttat
    15541 cctgatcaga aaactcagcc tatccaaaaa gccttttctc aaatcgaatg ggttaataat
    15601 cctgactggt ttcagttgtg gaaagaaggt aaaacgggtt accctattgt cgatgctgca
    15661 atgcttcagt tacaaaagac aggatggatg cataatcgtt tacgaatgat agtagcttct
    15721 tttttaacta aagatctttt atgtgattgg cgtttaggag agcagcattt tcaacaacaa
    15781 ctaattgatt atgatgctgc cagtaatatc ggaggatggc agtgggctgc ttccactgga
    15841 acagatgctg ttccttattt tagagtattt aatcctgtta ctcaaagtaa gcgctttgat
    15901 cccaaaggcg agtttattaa agcgtatttg cctcaactag aacacgttcc agagaagtat
    15961 ctccacgaac cgtggaagat gccaaaatac cttcaagaga gtgtgtcctg tattattggc
    16021 acagactatc ctcaaccaat tgtcgatcat gctaaacaac gtgaacaagc tattgcaaag
    16081 tatgagtggg caaaagaaaa agcaaaaata gaatgactgt tctcattata agagtttaaa
    16141 caaaaagtta tcatgaatta agattattta atttaaaaaa caagttgact ttatttagat
    16201 tatttgatac tattcagttg tctcccttag agaaagcgat ttcttgtttt ggagctgatt
    16261 tttaccattt ggtcttagaa taggatgagt aatatgactt acaacgaggt atatcaagaa
    16321 tggttgcaca ataatgatct tagtgatgat attaaagcag atttagcagc cataaaagac
    16381 aatgaggctg agattcaaga tcgtttttac aaaacacttg aatttggaac agcagggcta
    16441 agaggaaaac ttggagcagg caccaatcgc atgaatacct atatggttgg taaggcagca
    16501 caagctttag ctaatactat tattgatcat ggacctgaag cagttaaaaa gggcattgcg
    16561 gttagttatg atgttcgcta tcaatctaga acatttgcag aattaacatg ctctattatg
    16621 gcagctaacg gtattaaggc gtacctttat aaagggattc ggccaacacc aatgtgttcg
    16681 tacgctattc gtgctttggg atgtatctct ggtgttatga ttacggctag tcacaatccc
    16741 caagcttaca atggttataa agcttattgg caagaaggtt ctcaaatttt ggatgacatt
    16801 gcggatcaaa ttgcacaaca catggctgca ctaactcagt atcaggaaat caaacaaatg
    16861 ccttttgaaa aggctctgga cagtggactt gttacttata ttgatgagag tattgaagaa
    16921 gcatataaaa aagaagtgct tggtttaacg attaatgata ctgacattga taagtcagtc
    16981 cgagtagtct atactccttt aaatggtgtt gggaatctac cagtgcgtga ggttttaaga
    17041 cgccgcggtt ttgaaaatgt ttatgtggta cctgagcagg aaatgcccga tcctgatttt
    17101 acaacagttg gctatcctaa tcccgaagtc cctaaaactt ttgcatattc agaaaaatta
    17161 ggtaaggcag ttgatgctga tattttgatt gcaactgatc cagattgtga ccgtgtggct
    17221 ttagaagtta aaaacgcagt cggtgactat gtttttctca atggcaataa aatcggagcg
    17281 cttttatctt actatatctt ttcacaacga tttgacttag gcaatttacc ggctaatcct
    17341 gttttagtga aatccattgt aacaggagac ttgtcacggg ctattgctag ccattatggt
    17401 attgaaaccg ttgaaacatt gactggtttt aagaatattt gtggcaaggc aaacgaatat
    17461 gatgtgacga aacagaaaaa ctatctgttt ggttatgaag aaagtattgg tttttgctat
    17521 ggcacttttg ttcgtgacaa agacgctgtt agcgcctcta tgatgattgt agaaatggca
    17581 gcttactaca agaaaaaggg gcagaattta ctagatgttt tgcagactat ttatgcgaca
    17641 tttgggtatt ataatgaacg tcaaattgcc ttggagcttg aggggataga aggacaaaaa
    17701 cgcattgcga ggataatgga agatttcaga cagacaccaa tagctagtgt tgcagagatg
    17761 gcgttagaca agacaattga ttttattgat ggctatcaag attttccaaa gcaaaactgt
    17821 ctcaaatttt atttggatga tggttcttgg tatgcactta gaccatcagg aacagagcct
    17881 aaaataaaat tttatttata tactattggc caaacacaag aaaatagtgc aacaaaatta
    17941 gatgctattg aagcagcgtg tcgcacaaaa ataaatcagg ttaattaaac atcaaaaatg
    18001 gataacactt ttacactatt tttccatata aacaactaca aaaggaggtg agacttttag
    18061 gcaccttctt tttcttgtag gtgtcactaa tcaatttaat cgactcaaac caatactaaa
    18121 aaataagtaa gagtataaag aacaggcatc tatttttttg ttataataga aaaatgaaat
    18181 taaagggaga tgtcaatgac agaactaatt gatggtaaag ccttagctca aaagatgcaa
    18241 caagagttag cagctaaagt caacaatcta aaacaaaaaa aaggaattgt accaggctta
    18301 gccgttattc ttgtaggtga tgatcctgct agtcaggtgt atgtccgtaa taaagagcgt
    18361 gcagctctta ctgtaggttt taaaagtgag accgttagat tatcagaatt catttgtcaa
    18421 gaagagctta ttgcagtaat cgaacgttac aatgcagata acactattca tggtatttta
    18481 gtgcagctac ccctgccaaa tcatattaat gataaaaaaa ttattctcgc cattgatccc
    18541 aaaaaagatg tggatggttt tcacccgatg aatacaggtc acctttggtc aggacgtccc
    18601 ttgatggttc cttgtactcc atcagggatt atggaattgc ttcgagaata taatgttaac
    18661 cttgaaggta aacatgccgt cattattggc agatcgaata tcgttggaaa accaatggca
    18721 cagctcttac tggacaaaaa tgcaacagtc acgttgacac attcaagaac acgtcaatta
    18781 gaagaagtat gtcgctgtgc agatgtgttg attgtggcaa tcggacaagg tcatttcata
    18841 acaaaacaat atataaaaga gggtgcgata gtgattgatg tgggaatgaa ccgtgatgat
    18901 aatggcaagc taattggaga tgtggccttt gatgaggtgg cagaagttgc agcgaaaatc
    18961 acccctgtac caggaggtgt cggtcctatg acgattgcta tgttgctaga gcaaacttat
    19021 caatctgctc tccgtagtac tcataaatag ttatcattag acaattcaac ataagagaat
    19081 ataagaagtc agcaatggct tccttttttg tttttctgct cactatttat ccagaaaaat
    19141 ggtataattg cattagtatg caaagggact atgtcaggtc caactaagtg aaaggtggct
    19201 tgatggcaga ttatttaacc gtcactcatt tgacgaaata tttaaaatta aaatttgacc
    19261 gtgaccctta tttggaacgg gtttatctga ctggtcaagt gtccaatttt cgaaaacgac
    19321 caactcatca atatttttcc ttaaaggatg aaagtgctgt gattcaagcc accatgtggg
    19381 caggagtcta taaaaaacta ggattcgacc tagaagaagg catgaaaatt aatgtaattg
    19441 ggcgagttca actttatgaa cctagtggct cttactctat cgtgattgaa aaggcagagc
    19501 cagatggtat aggtgccttg gctttgcagt ttgaacaatt aaagaaaaaa ttaacggcag
    19561 aaggttattt tgagcaaaaa cacaagcagc ctttgccaca gtttgtatca aaaattgggg
    19621 tcattacaag tcctagtggt gctgtgattc gagatattat cacaaccgtt tcacggcgtt
    19681 ttccaggggt tgagatttta ttattcccaa ctaaagtaca aggtgatgga gccgcccaag
    19741 aagtagtggc taatattcga agagccaatc aaagagagga tttggacttg ctcattgtcg
    19801 gccgtggagg tggctcgata gaagaccttt gggccttcaa cgaggaaata gtggttcagg
    19861 ctatctttga atcgcaactt ccagtgattt cgagtgtggg tcatgaaaca gacacgactt
    19921 tagcggattt tgtggcagat cgcagagcag ccacaccaac agcagcagca gagttagcaa
    19981 cgcctattac aaaaacagat cttatgtctt ggatagtaga gcggcaaaat cgttcctatc
    20041 aggcctgctt gaggcggatt aagcaacggc aagagtgggt ggataaactc tcgcaatctg
    20101 ttatttttag gcaaccagaa cgattatatg atgcctacct gcaaaaaatt gatcgcctta
    20161 gcatgaccct gatgaatacg atgaaagatc gtctgagttc cgccaaagaa aacaaggttc
    20221 agttggacca tgccttggcc aatagtcaat tacagacaaa aatcgagcga taccaagacc
    20281 gtgttgctac cgctaagcgt ttactgatgg ctaatatggc tagtcaatat gatagtcagc
    20341 tggctcgctt tgaaaaagca caggatgctt tgctatcact ggataccagc cgaattatcg
    20401 cccgtggtta tgcaatgatt gaaaaaaatc aagcactggt agcctctgta agtcagataa
    20461 caaagggtga tcagttaacc attaagatgc gtgatggaca attagatgta gaggtaaaag
    20521 atgtcaaaaa cgaaaacatt tgaagaaaat ttacaagatt tagagactat tgtgaacaaa
    20581 cttgaaaatg gggatgtccc tttggaagaa gctatttcag aatttcaaaa aggaatgctt
    20641 ctctcaaaag aacttcaaaa gaccttacaa gcggcagaaa aaacactcgt caaagtgatg
    20701 caagctgatg gcacagaagt agatatggat gattaatgga caaattagct agaattgacg
    20761 aagccattcg tcgctactat aagacgacta gtaacggtgt atctgaagag ttgattgatg
    20821 ctatcttgta ttctgttgac agtggtggca agcgcattcg accacttatc ttattagaga
    20881 tgattgaggg atttggggta tcgttacaga atgctcattt tgatttggct gcagcccttg
    20941 aaatgattca tacaggaagt ctgattcacg atgatttgcc agccatggat aatgatgatt
    21001 accgacgtgg tagactgacc aatcacaaac aatttggaga agccacggcc attttggcgg
    21061 gagatagctt atttttagac ccctttgggt taatagctca agctgaatta aatagcgagg
    21121 ttaaagtagc cttaatccaa gaactttccc tagcttcagg aacttttggc atggttggtg
    21181 gtcagatgct agatatgaaa ggggaaaatc aagctttgag tctccctcag ttgtcactga
    21241 ttcacctcaa taaaaccgga aaattattaa cttttccttt taaagcagca gctcttgtaa
    21301 cagaacaggc tattactgtt cgtcaacaac tagagcaggc gggaatgctc atcgggcacg
    21361 cctttcagat tagggatgat attttagacg tgacagctag ctttgaagat cttggaaaaa
    21421 cgcctaaaaa ggatttattt gcagaaaaag ctacttaccc aagtttattg gggttagaag
    21481 cctcctgcca actgttgaca gagagtttag atcaggcttt gacgattttt cagacactcg
    21541 aaagcgatgt aggctttaag cctcaaataa ttacaaaact gatagaaggg ttacgactta
    21601 atgtctaaag aaagagtaga tgtgttagcc tataagcaag gattatttga gaccagagag
    21661 caagctaagc gtggtgttat ggctggctta gtggtctctg tgattaatgg ccaacgctat
    21721 gacaagccag gtgacaaaat tgacgatggg actgagttaa aacttaaagg tgaaaaactc
    21781 aaatacgtca gtcgtggtgg attaaaactg gaaaaggggc tgcacgtttt tggtgtatca
    21841 gttgctaatc aaattgggat tgatattggc gcttcaacag gtggctttac cgatgttatg
    21901 ttacaagatg gggccaaact agtctatgcc gtcgacgttg ggactaacca gttggtatgg
    21961 aaactcagac aagatccacg agtaagaagt atggaacagt ataactttcg ttatgctcag
    22021 ccagaggact ttaatgaggg acagcctgta tttgccagta ttgatgtgtc ctttatttct
    22081 ctcagcttga ttttgccagc cctgcataac gttttgtcag atcagggaca ggttattgct
    22141 ctcattaaac cgcaatttga agctgggcgc gagcagattg gtaaaaaagg catcgtcaaa
    22201 gacaaacaga ttcatgaaaa agtgattcaa aaggtcatgg attttgcctc aggttacgga
    22261 tttacagtta aggaacttga tttttcccct attcagggtg gtcatggaaa tatcgaattt
    22321 ttagctcatc ttgttaagtc acagacacct gaaacgttag ccccacatct gattcaggaa
    22381 gttgttgcca aggcacataa ggagtttgag aaacatgaaa aaaagtgaac gacttgaatt
    22441 aatcaaaaaa atggttttga cgcatccaat tgagacgcaa catgacctct taagattatt
    22501 ggcagagcat gggttagaat taacccaggc tactatttca agagacatga atgaaattgg
    22561 tatagtaaag attccttctg gcagtggacg ttacatctat ggtctttctc aagatagtgg
    22621 aaaaaagatc gttcaaggac ctagatcgat aaagagcact attttagctg tatctgacaa
    22681 aacaaaaggt ttagaacaac acctctattt aaaagtcgta cctggcaata gtaaattaat
    22741 caaacgttat ttattagcag atttttcaaa agctattttt agtcttattg ctgatgatga
    22801 tagtttattg ttaattgcaa agtctccttc agaagcagat atgatacgcc aagaaatttt
    22861 gctttggatg cagggtataa cctagaaaaa ataggggaga aagtgatgtt attagaaatt
    22921 tccattaaaa attttgctat tattgatgaa atttccctaa attttgaaaa tggtatgacg
    22981 gttttgacgg gtgaaactgg agcaggaaag tctattatca ttgatgctat gaatatgatg
    23041 cttggtgcgc gtgctagtac agaagtgatt cgtcgtggag ctaataaagc agaaattgag
    23101 gggttctttt cggtagatgc taccccagaa ctggttgcgt gtctggaatc atcaggtatt
    23161 gctatggaag aagaactgat tattcgccgg gatatctttg ccaatggcag aagcgtgagc
    23221 cgcattaatg gtcagatggt taacctagcc accttaaaac aggtcggaca gtttttggta
    23281 gatattcatg ggcaacatga ccaagaagaa ttaatgcgac cacagctcca ccagcaaatt
    23341 ttggacgctt ttggtgataa ggcttttgag caattgaaag agaactatca gcttattttt
    23401 gatcgttata aaagcttgcg tcgccaggtt attgacaaac aaaaaaatga aaaagaacat
    23461 aaagatcgta tcgacatgtt ggcttttcag atagcggaga ttgaagctgc tgccttgagt
    23521 cgaggtgagg acgaccggtt aaatcaagaa cgtgatcgct taatgaacca caaacaaatt
    23581 gctgataccc tgaccaatgc ctacgtcatg ctagataatg acgatttttc aagtctatcc
    23641 aatattcgct ctagcatgaa tgacttacta tcaattgagc agtttgattc agagtacaaa
    23701 gggatgtcga cttcgatttc tgaagcctat tatattcttg aagaagtgag caagcaatta
    23761 tcagatacca ttgaccaact ggattttgat ggtgggcgat tacaggaaat tgaatttcgc
    23821 ttggatacac tcaatagctt aacccgtaaa tacggtggta atgtgaatga tgtgcttgac
    23881 tactacgata atattatcaa agaataccag ctattaacag gagatgactt gtcttcaggg
    23941 gatctagagg cagaattgaa aagcttggaa aaacaattgg ttgctgctgc ttcgaagtta
    24001 agtgtctctc gccatcaatt ggcggagcaa ttagaagctg agattaaagc tgaacttaag
    24061 gaactttata tggaaaaagc agatttcaaa gttcacttta ccacatcaaa attcaatcgt
    24121 gatggtaatg aaagcttgga attttacatc tctactaatc caggcgaagg attcaagccc
    24181 cttgtcaagg tggcttctgg aggagagtta tcacgcctta tgttagccat caaggctgct
    24241 atttctagaa aagaagataa gacgagtatt gtttttgatg aagtagatac aggtgtttct
    24301 ggtcgcgttg cgcaagccat tgcccaaaaa atttataaaa ttgggcgaca tgggcaagtt
    24361 ctcgctattt cacacttacc tcaagttatt gctattgctg attatcagta ttttatttct
    24421 aaagaaagta aagaagagtc caccgtttct aaggtaaggt tattaactcc tgaagaacgt
    24481 gtggaagaaa ttgctagcat gattgcagga acagatatga cacaagctgc tcttacgcag
    24541 gctcgtgaac tacttgccaa acattaagat tatgtttggt atttgatatt ttcataatta
    24601 gatgacattg attttttagg tgctaaaata atcattgtgt cagaccattt ttcctctgtt
    24661 aatctggctt ggtttgcctt gacaaacctc tgagaaatag tagaatagat aagagaattt
    24721 taagctgagg ggatagaaat gactagtaaa aaagcgtgtt tatcaagcat cattgtgtta
    24781 gcaagtttaa cgtgtggaaa tgatactgtt agtgccaatc atctctcagc aactggagat
    24841 aagtttgatg attgctcaac acttgttgaa aaagatgtgg cccctaaaga tgaacttgag
    24901 atgttagcat ggtcctcgtc tcaaacaact gatgatgctg acagagacta tgaagatttt
    24961 ctcgatgatg attcttttaa tgatgattct tttatttctc aaaatgaaac tgataagatg
    25021 tttgagaatt taactgatga taggttatta gatgaattag atgaagaaga tacaattgag
    25081 ccagagcaaa atgtaataat gcctagtgac gatgagctat ttgatttaac tgatgctgtt
    25141 gagacacgcc ttactgtttc tagtgctccc catttagagg ctgaattgcc gaaaccacat
    25201 ttgaggagcc tatcagatac agcactgcgg tctggtgaaa ttagaggaca tttagataac
    25261 aaactggacg ctttgtctgt aacagctaca aagttagcat taacgatggc tcaaaaattt
    25321 gatttgacaa cgcatgtcta ttctataggt gaaagcttta gtgaagtatt agctgctcat
    25381 tatgaagaca gaaaagcaga atcagctttt tctaagaaaa agagatttca ccttcctatt
    25441 gctactccag atgttgttat agaggagtta aggcgcctgg tctcttctat tggaagttca
    25501 aaagaagatg tttcagttcc ttataatcgg aagctaggta tggcagttgc aaaaagaaaa
    25561 atagccctgc cacaaacggg agaggagttc tcttattatc cagctttact tggtttaatg
    25621 atattaggat taacgccgat tatgatacca aagaagataa ataattaggt aataagttat
    25681 tgtagttgta aaagacgggg caaatgttca agcgtgaaaa ggttgacttt tgcctcgttt
    25741 tgtatctcgt tattgctaag gcattttctt cgagcagcta gtgctcgcca tacctatcta
    25801 ccgagtgact attcaatagc actaccagtt ttgctttatt tagcaaggag tgttagaata
    25861 aagaggtgag aaactagttt agaatggaaa aatacatggg aactataaaa attgttacag
    25921 attcatcaat aactattgaa ccagaattaa ttaaagcttt agatattact gtagtgcctt
    25981 tatctgtcat gattgatagc aaattatatt ctgataatga tttaaaagaa gaaggtcatt
    26041 ttttgagtct tatgaaggct agcaaatccc ttcccaaaac cagtcagcca ccggttggtc
    26101 tctttgctga aacttacgaa aatttggtca aaaaaggagt aactgacatt gttgctattc
    26161 acctctcacc agctttatca ggtactattg aagcttcccg tcagggggct gaaatcgctg
    26221 aggcacctgt tacagtttta gattcaggat ttactgatca agccatgaaa tttcaggtcg
    26281 ttgaggcagc aaagatggca aaagcgggtg ctagtttgaa tgagattttg gctgctgttc
    26341 aagctatcaa atcaaaaaca gagctctaca ttggtgtatc taccttagaa aatttagtaa
    26401 aaggaggtcg tattggtcgt gtgacgggtg ttttgagctc gctacttaat gtcaaagttg
    26461 ttatggcact taaaaatgat gagttaaaaa cgcttgtcaa aggtcgaggt aataaaacat
    26521 ttacgaaatg gttagatagt tacctcgcta aaaatagtca tcgcccgatt gcagagatag
    26581 ctatttctta tgctggtgaa gctagcctgg cattaacctt aaaagaaaga atcgcagctt
    26641 attacaacca ctctatttcg gttttagaaa caggctctat catccaaaca catacaggag
    26701 aaggagcttt tgcggttatg gttcgttatg aataatcgtc atttatttag tgggatattt
    26761 ttctttgtta ttagtttatg tctggccttt ttattgctaa atattattat ccctaagtca
    26821 aattcacgtt tgaaaaagag tgattttctg acaaaagaac aagtagctat ccaatatgtt
    26881 gctataggag attcattgac agaaggagta ggtgatctaa ctcatcaagg tggttttgtt
    26941 cctttgttaa cgaatgatct cagtgaatat tttaaggcta atgttaatca tcaaaattac
    27001 ggcgtatctg gtgataccag tcaacagatt cttgatagga tgataaaaca aaagcagata
    27061 cagttatctt taaaaaaagc agatataatg acgttaaccg ttggtggtaa tgatgttatg
    27121 gcagttattc ggaagaattt agcggatttg caagtttcta gttttagaaa gccagctcgt
    27181 cagtatcaaa aacgattaag acagattatc gagttagcca gaaaagataa taaagatctt
    27241 cctattttta ttttaggcat ctataatccg ttttatttga attttccaga actaactgat
    27301 atgcaaaaag tgattgatga ctggaatatc aaaactaagg aggttgttgg agaatacgat
    27361 cgtgtgtact ttgtgccaat aaatgacctc ttgtataaag ggataaatgg acaagaagga
    27421 attgtccatt cttcaggaga tcaaactaca attgtcaatg atgccttgtt tactggggac
    27481 cattttcacc caaataatac tggctatcaa atcatgtcaa atgcagtaat ggagaaaatt
    27541 aaaaagcatg aaaaaaaaat caaaccttaa ttggtggaag tggagcttct tatgtctgct
    27601 tgcttttaat accgcctttt tgatggttat tgcaagtcgt ctcattcaag taagagaacc
    27661 tgagtcagaa ctgattgcta aaaaaccagt taaaaatatc aaaattggta catttgtcac
    27721 aaccagagaa cagcttaacg aaactgtagc aagttatctt aaagattatc agactaaaaa
    27781 aatgtcttat aaattttatg cgacatcttc ttctattttg tttgaaggaa cttatcaatt
    27841 attagggtat gaagtccctt tatatatcta ttttcaacct catcgtctag aaaatggagc
    27901 tgtccaacta caggtgatct ctttttcagt gggaactctg ccactcccag aaaaagatgt
    27961 tttgcagtat ttgaaatcaa gctacaaatt gccaagcttt gttaaagtga tgccaaatca
    28021 atcagctatc gttgttaatt tacaagatat ccaaaatgat gctaaggttt atttaaaggc
    28081 caagaaaatt gatttattta atgatgaaat cagttttaac atctataaga aataagctca
    28141 aataacgtta aatacttgac ctaatcgctt tttttagata tgataatatc tgttaaagca
    28201 ttaagcttac taattatttg gaggatttgt taaatggcta acaaacaaga tttaatcgca
    28261 aaggttgcag aagcaactga attgactaaa aaagattcag cagcagcagt tgatgccgtg
    28321 ttttctacaa tcgaagcttt ccttgctgaa ggtgaaaaag tacaattgat cggttttggt
    28381 aacttcgaag tacgcgaacg tgcagctcgt aaaggtcgta acccacaaac tggtgcagaa
    28441 attgaaattg cagcttcaaa agttccagcc ttcaaagctg gtaaagctct taaagacgct
    28501 gttaaataat tcgtctagaa aaatcttgtt gctatcgatg tttattgata gcgacaaggt
    28561 tcttttttta tatttggggc ataaagttag tagttttctc ttcagttaag ctaagttcta
    28621 atgcatgcac tctataatgg tcagtcatcc ttctattagt atatcatttc cgcaggtagg
    28681 gggagttttt ttcatatttg caaatagttg aagttaggat ttttgaaagc aaaaggtaag
    28741 tttaaaaaaa gtttatttag aatgtttcta aattacttga cttatttatt tagaattatt
    28801 ataattaata tatcttaaag attgaataac ttttaattgc gagggaacta aaaatgacaa
    28861 tcagacaatg gatggcagac catcttcatt taatggagac tttagcgtgt ttggtattaa
    28921 taataattgg cctagccttt ttacactcat ttccacaagt ggcttccgct attttcatta
    28981 cagccttctt gattggagga tatgcgtctg caaaaacggg tatattggat ttggtgaaga
    29041 acaaacactt gtcagtggat attttgatga ttttggcagc tatcggtgct ggaattatcg
    29101 gctattggct ggagggtgct ctgcttattt ttatcttttc gttgtccaat acgcttgaag
    29161 aaatggccat ggaaaaaagt aaggatgcta tttcagcctt gatgtccttg acgccagata
    29221 cagctcgtca ataccaagaa gatggccata ttttagaagt tgagaccaga tccttgagtg
    29281 ttggtgaccg tttacaagtt cgtaaaggcg aagctgttcc aattgacggg cagttgctta
    29341 gtccttttgg tcaatttgat gaatctatgg tcactggtga gcctatcact gttgataagg
    29401 cagaaggcca ggatctgatt gggggaacca ttaaccaagg acaaaccata gatatgttgg
    29461 ttactattga aaatgacgat actctctttg ccaagattat taatctggtg gaatctgccc
    29521 aagaaaagaa aagcaaaacc gccaccttta tcgaaagctt agaagatggt tatgtcaaat
    29581 ttgtgctcgt ccttattcct gcctttatcc tctttagcca ctttgtgttt tcttggactt
    29641 ggttggctgc tttttaccga gggatgattc tcttaacagt agcttcacca tgtgccctaa
    29701 ttgccagttc cacacctgct agcttggctg ctatttctcg tgcagccaga aagggattga
    29761 ttatcaaagg aggggatatt gtcgataaca tgggagatat taaggctgtt gtcatggata
    29821 aaacgggaac gctcacccaa ggaaaacctt ctgttgtgaa tgctcattat ttggaagatg
    29881 agttgctcgt gaatagactg gtaaaaggag cagaggctgc tagtacccat cctatttcta
    29941 aagcccttct tgaatacact gaaaaattgg agccactgac ctttgaccac ttagaagaaa
    30001 tttctgggaa aggttttcag ggcttctatc aagggcaaga atggcgaatt ggcaagaaaa
    30061 ccttcatttt ggaaaaggtt caagacctat cagcttttga agaaactatt caagtggaag
    30121 aaaatcaagg gaaaacccta atctttgttt cacgtgacca tcaattgata gcttactatg
    30181 ccctcttgga tgatatcaaa atagaatcaa aacgtgctat taagtctctt catgccatgg
    30241 gaatcaaaac agtcatgtta acaggtgacc aagaacgaac cgccaattat gtggcacaaa
    30301 aacttggtat tgatgaagtg gtagccaact gtatgcctca agataaggtg gctaagttag
    30361 cagaattaaa gaccaaatat ggttttgtgg ccatggtagg agatggtatt aatgatgctc
    30421 ctgcccttgc tcaagcagat gtttcttatg ctattggatc aggaacagat attgcaatgg
    30481 aaagtgcaga cagtgtgatt atggatgact tgactcgtat tccattttcg attcaacttt
    30541 cccgcaaaat gaagaccatt atcaaacaaa atattgtttt tgccttatct gtgattacct
    30601 tattgatttt agccaatgtt tttcaggtag ttaacttgcc gcttggtgtt gttggacacg
    30661 aaggctcaac gattttagtg attttaaatg gcttgcgttt actttctttt aaataattta
    30721 gaaacttgct tgaaaacttg aaagtgttta taataatagt aagacttatg aaagagaaaa
    30781 ccgccgtatg tttactatta tgatcattgg tttagctgta ttttcaataa ttaaaatcat
    30841 tctatttttc tatttttatc agcaatttga gcgcaagcaa aaaagaaaag ttgaatcaaa
    30901 caaccagcaa agacagtccc aagaaagtga cgctgtccct tcaaaaaaat tcccatttta
    30961 aaaagcttgg aaagtgtaca aagtgcacat ttccaagctt ttttgactat taaggttaat
    31021 ctttgtaacg taagttccca cgaaaatcgt ctaagctttg gtagcctttt tctaccataa
    31081 tcgtttttag ttccttagtg acccgttcaa agatagctgg cccttcttga tgaagggctg
    31141 tcccaatttg gaccatgctg gcgccacaaa gaatatgttc aaaggcatcg cgtcctgttt
    31201 taactccccc tgtaccaata atgtggatag aaggttttaa gcgtttgtaa aacgcatgga
    31261 cattggctag agcagtaggt ttgatgtaat ctccacctat accaccaaag ccatttttag
    31321 gcttgatgag gacttgttca tcctcaatga ccagcccatt tccgatagaa ttgacacagt
    31381 taacaaagga aagggggtat ttattgaaaa tcgcagccgc ttgatcaaaa tgcacaatgt
    31441 caaaataagg aggtaatttg ataccaagag gtttagtgta ataggtaaag atgttctcta
    31501 gaagttgatc agtggtttca aaatcataag caatctgtgg ttttcctgga acgttagggc
    31561 aagaaaggtt tagttccact agaccctcat aatctgaagc catgatggct tttaaaatgg
    31621 tttcggtttc agtaggagat agcccgacaa cggagagaaa gtgaggttta tgttgcccgg
    31681 ttttggcaag atcactgaca aagtctaagt agtagcgaaa accattatta gggagtccca
    31741 ttgaattgat agaaccaaga cgagtatctg cgtagcgagg ttctggatta cctggacgaa
    31801 cttcaagcgt ccctgttttg gtgacaaagg aagcggcttg agacttttca acctccataa
    31861 gctcttcttt tgtcatgcag taaacaccag cagcattcat caaacagtta tcaaaagaga
    31921 aatgactaat ttgtgtagca gtagaaacca ttatttcctc catattttag acaaattttt
    31981 tctattctac cactaggcat gaataatagc aagctatttt tacaaaaatg taaatatatt
    32041 actgttattg acgaacattt gaaattttca tgatagtcac attatgattt tcaataaagt
    32101 cagaatagac tagctttttt gctatatttt ttgtaaaata ggaattgtaa aaaggtagca
    32161 acctttaaat taaaaggaga tattaactca tggtaaaatt ggttttcgct cgccacggtg
    32221 agtcagaatg gaacaaagct aacctcttca ctggttgggc agatgttgat ctttcagaaa
    32281 aagggacaca acaagcgatt gatgcaggta aattgatcaa agaagcaggt attgaatttg
    32341 accttgcttt cacttcagta ttgacacgtg ctatcaaaac aactaacctt gcccttgaaa
    32401 atgcaggtca attgtgggtt ccaactgaaa aatcatggcg cttgaacgag cgtcactatg
    32461 gtgctttgac tggcaaaaac aaagctgaag ctgcagaaca atttggtgat gaacaagttc
    32521 atatctggcg tcgttcatac gacgtgttgc cgccagctat ggctaaagat gatgagtatt
    32581 cagcacacaa agaccgtcgt tatgctgatc ttgacccagc ccttattcca gatgctgaaa
    32641 acttaaaagt aactcttgaa cgcgcaatgc cttactggga agaaaaaatt gctccagctc
    32701 ttcttgacgg taaaaacgtc tttgttggtg cacatggtaa ctcaatccgc gctcttgtga
    32761 aacacattaa aggtctttca gatgacgaaa tcatggatgt ggaaattcca aacttcccac
    32821 cacttgtttt tgaattagat gaaaaactta acattgttaa agaatactac cttggtggtg
    32881 aataacttaa aaaccagact ttggtctggt tttttgggtt ctatatccta agaagaaaga
    32941 aaaccctgct taaaaatatg atatgataga aataaaaaag gatagataga ttatgaaagc
    33001 agaagatcgt cgtcaaaaaa ttattgagtg cttaaatagt gaacaaaaag ctgtatctgc
    33061 tactcgttta ggtaagttat taggagtgag tcgccaagta attgttggag atatagctct
    33121 tcttcgagct cagcagattg atattatttc gactccaaaa ggttatatta tgtcaacagc
    33181 gctttatagt caccaatttt gtgcacgtat tgtgtgccaa cataatgtag aagaaactaa
    33241 aaaagaatta gaaattattt tagctcatca agggattatt acgacggtag aggtagaaca
    33301 tcctatttac ggtatgataa cagccccttt aaatattaag acacactctg atgtaactaa
    33361 tttcatgtcc aaactcagtc aatctaaagc agagttattg tcttcgttga cagaaggact
    33421 acatagtcac ttaatttcgt gtcctagtca agaagccttt ttagctatca agcatgattt
    33481 agaattagca ggtatcttat acaaaggaat ataactacag tatattatgc cattgatggt
    33541 tgttatgaat accatgctaa aatattctat tgattattta tcagcaacaa ggtatcgttt
    33601 aaaaatacca aaagatacag gtgtcttgat agttattggt ttttaaggta tactattttt
    33661 gaaaaggaga taaaatgact aaaaaaccaa cacaactaat agcttatacc tctattttgg
    33721 tcgcatttgc gattttgatt ccgataatca tgcctcttaa gcttattatt ggaccagctt
    33781 cctttactct ggcgagccat gttcctttgt ttttggctat ttttatgtcg attccagtag
    33841 caattttggt cgctcttggt accacattag gttttttatt agctggactt cctttaatta
    33901 ttgttttaag agcactctct catttattgt ttgctatttt ggcagcgtgg tggttaagta
    33961 gaaaaccaca actgatgaca tcggcagtaa aatgttttag ttttgcgttt ttcattaatg
    34021 tcattcatgg cttggctgaa tttttagttg tctatatttt aacagcaacc acagcaactt
    34081 ctatgtctta tttttggtct atgctaggct tgattggctt aggttcgctt attcatggca
    34141 tattggattt ctatcttgct ttagttctat ggcgtttttt agcaaaaaac ttaaaacttc
    34201 ccaccataaa tgcctaagac taaaatagag atagttatat gataatgacg agccctttct
    34261 tgttgaaagg gcttgcattt tttaggaaaa ttgatatact aaagtgtgtg tttgtgaata
    34321 cagtaacaaa aggagatatc atgaaaacac cagaacagat tttagaagca actattcata
    34381 ttggggaaca taaggttacc aaaacttttt tggctaaatc tatcttaggc tttattggtg
    34441 gagcgatgat tagccttggt tatttactat acgtgagaat tgccgccagt ggtttagaaa
    34501 cttttggtgc tttttccagt atcgtaggag cttgtgcttt tccgattggt ctaattatta
    34561 ttttaatggc gggtggagaa ttaattacag ggaacatgat ggctgtttct gcagctttgt
    34621 tggccaaaaa aattaagttt agcgagcttg ccaagaattg gcttatcatt accttgttta
    34681 atgtgattgg agctgttttt gtagcttttg tgtttggtca ttttctaggc ttgacatcag
    34741 ctggtatttt taaagaagaa gtgattgagg tggcccatgc taaaatagca gcgagccctt
    34801 tgcaggcact agtatcagga attggttgta actggtttgt tggtttggcc ttgtggctat
    34861 gctatggggc aaatgatgct gctggtaaat tccttggtac ttggtttcct gtcatgacct
    34921 ttgtagccct tggttttcag catagtgtag caaatgcttt tgtcattcct gctgctattt
    34981 ttgaaggagg agcaacatgg ttggattttg tcactaattt tatttttgtc tatagtggta
    35041 atattatcgg tggagccatt tttgttagtt ttttatattt taaagtttac tatcatccgc
    35101 aaaagagtaa aactcaatga gaaaatattt ttttagactg agggcgtcct attctcagtc
    35161 ttctttttat aatttatggt aaaataagaa gcgaagttta aaaaatttag ctaaatatgg
    35221 ttagtttatt ccctaaaaaa ggagtattcc caaaaaatcc gtaaaaagaa tttgctatgg
    35281 gagtagttcc caaaataatg cagatcatta gttgtctttc aaaaatagcc ttgatagggt
    35341 aaaaagtgga cagtgatgca gcaacagatt catcaggcgt acaaactgtc ttatggtttg
    35401 tgcttagaga tttcttaata atgcttttgg taatacactt ttagtgaaga aagtgtagca
    35461 tgaaagctta aagataacca tgagtttgct ttatgcacta ttgttgagaa aaatagcata
    35521 gtcaattgag taaacatcat ttctgcatgg aatgaaacaa ttcctataaa cttatatcat
    35581 aaggatggtt tgcggtttct aaaaaagtaa catgaatcaa aaaacaaaag ccaaatatcc
    35641 caaggccaag gtaacaagtc gagtaaaaaa gtttttactt ggctaatcca aacggttctg
    35701 tcacgattca cgctaaaatt ggaactgctg agacttgtat cacagacaat tcagatcaga
    35761 ccttagtaac ttacaactta aatgtagtag cttataatac aaataacgaa gataatattg
    35821 cggttgctgt acatattacg aatatatcgg caaagatatg agggcgcttt atgtggcgat
    35881 atatggaaaa caataaagga gaacttagtg ctttatccaa cacccattgc aaagttaatt
    35941 gacagttact ctaaacttcc aggaattggt atcaagacgg cgacgagatt agccttttat
    36001 actattggaa tgtcaaatga agatgtcaat gattttgcta aaaacttatt agcagctaaa
    36061 agagaactga cctattgttc gatttgtgga aaccttaccg atgacgatcc ttgtcacatt
    36121 tgcacagaca cgagccgtga tcagacgacc attctggtag tagaagatgc taaagatgtt
    36181 tctgccatgg aaaaaatcca agagtatcat ggctattatc atgtgcttca cggcttgatt
    36241 tcgcctatga atggtgtggg gccagatgac atcaacctta aaagtttaat tacccgttta
    36301 atggatggta aggtgagcga agttatcgta gctaccaatg ccacagcaga tggggaagca
    36361 acgtccatgt atatttcacg tgtcttgaaa ccagccggga ttaaggtaac tcgtttggca
    36421 agaggtctcg cagtaggttc agatattgag tatgctgatg aagtaacatt attgagagct
    36481 attgagaatc gtactgaact ttaatgatca tgttggtgcc tttactcgca aagagaggtt
    36541 ttgaaaaggc tacatctttt tgttataata aaaaaacatt caattcagat aagaaaggtt
    36601 agctatgtcc aaacaaacgt tagttctatt atacggaggc cgctcagcag aacgtgaagt
    36661 ctcagtgctt tcagcagaga gtgtgatgcg agcagtcaac tatgataagt tcttagtaaa
    36721 aacttatttt attactcaga tgggccaatt tatcaaaact cagcaatttt cagaaaagcc
    36781 atcggaatca gaacgcttga tgacaaatga gacgatagag ctaactcaaa aaataaaacc
    36841 aagtgatatc tatgaagaag gtgcagttgt atttcctgta ttacatggac ctatgggaga
    36901 agatggctct atccaaggtt tcttagaagt gcttagaatg ccatatatag ggactaatgt
    36961 catgtcttct agcatagcta tggataaaat tactactaaa cgtgtcttag aatcaattgg
    37021 aattccacaa gttgcctata ctgtttatat tgacggacaa gatttggagg cttgtttagt
    37081 tgaaacttta gcaagactta cttttcctat cttcgtaaaa ccagctaata tgggttcttc
    37141 tgtggggatt tccaaagccc aaacaaaagt agaattaaga aaagcgattc agttagctct
    37201 gacctatgac agtcgtgtct tgattgaaca aggcgtggtt gccagagaaa ttgaagttgg
    37261 tctccttgga aatgataagg ttaaatcaac tctaccagga gaagtaatca aggacgttga
    37321 tttttacgac taccaagcaa aatatgttga caataagatt acgatggcca ttccagcaga
    37381 tgtagaccag agtattgtga cagaaatgcg ttcttatgct gaagtagcct ttaaagcctt
    37441 aggtggttgt gggttgtctc gttgtgactt tttcttgacc caagacggac aggtttacct
    37501 taacgaatta aacaccatgc caggatttac ccaatggtct atgtatccac tcttatggga
    37561 aaatatggga ttggcttatc cagatttgat tgaagagctg gtgacactgg cacaagaaat
    37621 gtttgaccag cgtgagagcc acctgattta aacctatgag atgaataaat attgacttgg
    37681 atggaacggc aaacatcgtc aagatgtgac gttcgtcctt tttttgttgc tcttttagga
    37741 agaatagtag agccattaag ggagtttgac attttagaaa tttttctaat gttttgtgct
    37801 ataatagata ggacatcgta aatactagtg ttcttagaaa gaaaacaaaa tgaaattaac
    37861 cttacacgaa gtggctaaaa tagttgacgc ccaaaataat gtgtctgact tagacgacgt
    37921 tcctctgcat cacattgaat ttgatagccg aaagattaca aaaggagacc tctttttacc
    37981 cttaaaaggt caaagggatg gtcatgagtt tattgattta gcctttcaaa atggagctgt
    38041 cgctactttt tcagaaaaag aattgccggg gacacctcac cttcttgttg aagactgcct
    38101 aaaagctttt caaaagctag cccattatta tattgataag atgcgcgtgg atgttattgc
    38161 tgtgacaggc tcaaatggta aaacttccac caaagatatg ataggagctg tcctatcaac
    38221 cacttacaaa acttacaaaa cgcaaggaaa ttacaataat gagattggtt tgccttacac
    38281 cgttctccac atgcctgacg atacggaaaa aattgttctt gaaatgggac aagatcacat
    38341 gggagatatt cgtcttctgt ctgaaattgc aaggcctcgt attgcagttt taaccttagt
    38401 cggcgaagct catttagaat attttggaag ccgagacaaa atagcccaag gcaaaatgca
    38461 gattgttgac ggcatgaatt cagatgggat tttaattgct ccgggagatc cgattattga
    38521 cccatatctt ccagaaaatc agatggttat ccgttttgga aatcagcagg aaattgacgt
    38581 tactggtatt caagaagaca aagatagctt aacgtttaca acaaatgtgt tagcaacacc
    38641 tgtgtcccta cctctaccag gaaaatacaa cgcgaccaat gctatggtag cagcctatgt
    38701 aggaaaacta ttggcagtaa cagacgagga tattatagct gctttacaga ctgtgacgtt
    38761 gacaggtaat cgtacggagt ggaaaaaagc agccaatggt gctgatattt tatcagatgt
    38821 ctacaatgcc aacccaacag ccatgcgtct gattttagaa acatttgcta acatagcaaa
    38881 gaaccccgga ggaaaaaaga ttgcagtgct agcagatatg aaagaacttg gaaaggattc
    38941 agtgatactt cattctcagt taattgacag cttaacatca ggtaatattg atcagcttgt
    39001 tttttatggg gatcatatta aagaattagc acggttggca agtcaggttt atcctgctga
    39061 gcaagtacat tattttttaa aaactgaaca agaagaccag tttgaagcca tggcacaata
    39121 tgttcaaaac attcttaatc cctttgatca aattctttta aaaggcagcc actcgatgtc
    39181 gctagaaaag ttagtggaac gattagtgac tgacaagaat tgtgacagct atcagccttg
    39241 atttttataa aactttcaga gaaaggcttg ttctctcatg aagattaagg tataatgaaa
    39301 aaaatataga aagggacgat tatggatttt tttgcaattg accccatcgg actgcctcat
    39361 atctcactta tcttttactt gttgtcactc ttgatagcgc ttttgcttgt ttttttgact
    39421 tttcaagcat ataggttaaa atcttatcgt tattttttct tgtttttaca gttatctcag
    39481 gtgattggct tgtacacatg gtacgtgtta agggggtttc ctttagatga agctttgccg
    39541 ttgtatcatt gtcgcatcgc tatgttagct atcttttttc tacctgatcg taacaagttt
    39601 aagcaattat gtatggtttt ggggataggt ggaacattcc ttgcactttt atcgccagat
    39661 ctttatccgt ttagattatg gcatgtagca aatgtctcgt tttatcttgg tcattatgct
    39721 ttgttagtga atggtttgat ttacctcttg cgtttttacg atgccagtca gttaagactg
    39781 ctatcagtag tacgctattt agcaactgtt aactttcttc ttctgttggt cagtttagcc
    39841 acgaaaggaa attatggttt tgtgatggat attccagtga ttcatacgcg tcatttgcta
    39901 cttaattttg tgatcgttac aagtggcttg acttttatgg ttaaaataac cgaatatttt
    39961 taccttaaat ttggtgaagc tcaacagctg gcatttgctt tctctaaaga aaagtaggtc
    40021 gttgaaaaag cacttaaaat cagctataat aagaagagtc tgggatgatt tccaggctcg
    40081 atttttttaa aaaagacact cattgatagc ccaagataag ttataatacc agttttgaag
    40141 aactttagag gctaatcgta tatggagaaa cccaatgtag cccaactctt ttgataaaca
    40201 aataatacct aacgaaaata actcagagaa aagctctgcc attttactat acaaggaaaa
    40261 aagaatgtca cttacagaag aaattaaaaa acgccgtact tttgccatca tctctcaccc
    40321 ggatgctggt aagacgacta ttactgagca actattatat tttggtggtg aaattagaga
    40381 agcagggaca gtaaaaggga aaaaatcagg tacgtttgca aagtccgact ggatggatat
    40441 tgaaaagcaa cgtggtatct ctgttacttc atctgttatg caattcgatt acgcgggtaa
    40501 gcgtgttaat attttggata cacctggaca tgaggatttc tcggaagata cataccgtac
    40561 cttaatggct gtggatgcgg ctgtcatggt tgtagattcg gctaaaggta ttgaggcgca
    40621 aacgaaaaaa ctttttgaag ttgttaagca tcgtaatatt ccggttttta cgtttattaa
    40681 taaattggac cgtgatggac gtgagccttt agaactcttg gaagaattag aagaagtttt
    40741 agggattgct tcttatccga tgaactggcc gattggtatg ggacgcgcct ttgagggctt
    40801 atatgattta cataataaac gtttagaact ctataaggga gacgagcgtt ttgcaagtat
    40861 tgaagacggt gatcagctgt ttgccaataa tcccttttat gaacaagtca aagaagatat
    40921 tgaacttctt caagaagcag gtaatgactt ttcagaacaa gctattcttg atggtgacct
    40981 aactccagtc ttttttggct ctgcccttac gaactttgga gttcagactt ttcttgatac
    41041 tttcttggag tttgccccag aacctcatgg tcacaagact acagaaggta atgttgttga
    41101 ccccttagct aaagattttt caggttttgt ctttaaaatt caagctaaca tggaccctaa
    41161 acaccgtgac cgtattgctt ttgtgcgtat tgtttctggt gaatttgagc gcggtatggg
    41221 agttaatttg acacgcactg gcaaaggcgc taagctctcc aatgttactc aatttatggc
    41281 agaatcacgg gaaaatgtga cgaatgctgt tgctggggat attatcgggg tttatgatac
    41341 aggaacttat caagttgggg ataccttaac agttgggaaa aataaatttg aatttgaacc
    41401 cttaccaact tttaccccag aaatcttcat gaaggtttct ccgaaaaatg ttatgaagca
    41461 gaaatccttc cacaaaggta ttgaacaatt agtacaagaa ggagcaattc agctttataa
    41521 gaattatcag acaggcgaat acatgctggg tgctgtgggt caactgcagt ttgaagtctt
    41581 taaacatcgt atggaaggtg aatacaacgc tgaagttgtg atgacaccaa tgggtaaaaa
    41641 gactgttcgc tggatttcag aagatgattt ggatcaacgc atgtcctcaa gccgaaacat
    41701 tttagctaag gatcgttttg accaaccagt tttcctattt gaaaatgatt ttgcccttcg
    41761 ctggtttgca gacaaatacc cagatgtcac tttagaagag aaaatgtaac cgaaacatga
    41821 ttttcaacag tctaacgttt tggagatcaa tttactgagt aactttttaa aagaaatgag
    41881 tggctagtta tctcttttgc agatttaaga ttctatcaaa aaacgatatc ttcacaactg
    41941 taagcatatt tatgatttag cggataatcg tgaaagcgtg ttattgctta tattagcctt
    42001 atttcctatc tttttgattc taattgtgtt acactagtta ggttgttaat caaacgacga
    42061 aaattaaaag gtttataagg cagcttcgca ctgccttttt agaaaagaga aacaattgaa
    42121 atttacagaa tttaacttat cacaagacat ccaatcagct gttgttaccg ctggttttga
    42181 gaaagcatct cctatccagg aaatgacgat tcctctcgca ctagaaggga aggatgttat
    42241 tggtcaagca caaacaggaa ctggtaaaac agcagcgttt ggtctaccaa ccttgaataa
    42301 gattcgtact aacgaaaata tcatccaagc attggtcatt gcaccaacgc gtgaattggc
    42361 tgtacaaagt caggaagaat tgttccgttt tggacgtgaa aaaggggtta aggttcgctc
    42421 tgtttacggt ggatcaagta ttgaaaaaca aatcaaagcc cttaaatcag gtgcccacat
    42481 tgtcgtggga acaccgggtc gcttgttaga tttgattaag cgcaaggctc ttattttaga
    42541 tcatgtggaa accttaatcc ttgatgaagc agatgaaatg cttaacatgg gattcttgga
    42601 agacattgaa gctattatta gtcgtgtccc agcagaccgt caaaccttgc tcttctccgc
    42661 aacgatgccg gcacctatta aacaaatcgg ggttaaattc atgaaagacc ctgaacatgt
    42721 tcaaattaag aacaaagaat taacaaacgt caatgttgat caatactatg ttcgtgtgaa
    42781 agaacaagaa aaatttgata ctatgacccg tctgatggat gttaaccagc cagaattatc
    42841 catcgttttt gggcggacta agcgccgagt tgatgaaatt acacgtggct tgaaacttcg
    42901 tggtttccgt gccgaaggaa tccatggtga tcttgaccaa aataaacgct tacgtgttat
    42961 ccgtgatttc aaaaacgacc aaattgatat tcttgttgcc actgatgtag cagcgcgtgg
    43021 ccttgatatt tcaggagtta cccacgttta taactacgat attacccaag atccagaaag
    43081 ttatgttcac cgtattggtc gtacaggtcg tgccggtaaa tctggtgagt ctatcacttt
    43141 tgtctcacca aatgagatgg gctacctctc aatgattgag aacttaacga aaaaacagat
    43201 gaaacctctt cgtccagcaa ctgctgaaga ggcctttcaa gccaagaaaa aagtagccct
    43261 caagaaaatt gaacgcgact ttgcggatga gactattcgt tcaaactttg ataaattcaa
    43321 aggcgatgct gtccaattgg ctgccgaatt cactcctgaa gaattagctt tatatatttt
    43381 gagcttaacg gttcaagatc cagatagctt accggaagtt gaaattgcac gtgaaaaacc
    43441 attaccattc aaatatgtgg gtggtggtca cggcaataaa aatggcaaag gtggtcgtgg
    43501 acgtgacaac cgtaaccgtg gtgatcgccg tggcggctat cgtggtgacc gtaacagaga
    43561 tgagcgtgat ggtgaccgtc gtcgtcaaaa acgtgataag cgtgatggtc acgatggcag
    43621 tggcaaccgc gatttcaaac gtaaatcaaa acgtaatagt aaagactttt tcaataaaga
    43681 gaaaaaatca agtgctaaaa atactggttt tgtgattcgt cacaaaggtg aataagctta
    43741 aagagagttg atgagagtca gctcttttta attttctgaa aaagttgctt ttataatggg
    43801 atctgttata atggtttcag ttatttcatt ataattttta aaaaattagg aggagtataa
    43861 gatgtctgat agccatctta ctgcctttga caaagcttca aaagcaggtt ttattattgc
    43921 tttaggtatt gtttatggag atattggaac aagccccctt tatacgatgc agtctctagt
    43981 tgaaaatcaa ggaggggtta atcaggtttc tgaatccttt attttaggat caatctcttt
    44041 aattatttgg acactgacct tgatcacaac aatcaagtac gttttgattg ctctaaaagc
    44101 tgataaccac catgaaggtg ggattttttc tttattcacc ttggtacgta aaatgtcacc
    44161 gtggctcatt gttccggcga tgattggagg tgcaacactt ttgtctgatg gggctcttac
    44221 acctgcggta acagttactt cagctattga gggcttgaaa gccgttcctg gtctgagtca
    44281 catctaccaa aatcaaacca atgtcattat cacaactcta gtgattttaa tagtcttgtt
    44341 tggtattcag cggtttggaa caggttttat tggtaaaatt tttggaccag ttatgtttat
    44401 ctggtttagt tttctaggtg ttagtggttt ttttaatatg ttggggcatc tggaaatttt
    44461 caaggctatc aatccttact atgctctgca tttgctgttt agtcctgaaa accatcgtgg
    44521 gatttttatc ttggggtcca tttttcttgc gacaacagga gctgaagccc tttattcgga
    44581 tttaggacat gttggtcgag gaaatattta tgtgagctgg ccctttgtta agatgtgtat
    44641 tgtgctgtct tactgtggcc aggctgcgtg gattttagcg aataaacact caggtattga
    44701 actaaaccct ttctttgcta gtgtgccatc acaattaagg gtttaccttg ttagtttggc
    44761 aaccttggca gctattattg cctcacaggc tcttatttca ggctccttta ccttggtttc
    44821 agaagccatg cgtttgaaaa ttttcccttt gtttcgtgtg acttacccag gggcaaattt
    44881 gggacaattg tatattcctg tcattaactg gattttattt gcagtgacct cttgtactgt
    44941 tttggctttt agaacatcag cccacatgga ggcagcttat ggtcttgcta ttaccatcac
    45001 catgttgatg acaacaatcc tcttaaagta ttacttaatc aagaaaggaa cgagacccat
    45061 cttggctcat ttagtgatgg ctttctttgc ccttgtagaa ttcattttct tcttggcttc
    45121 agctatcaaa tttatgcacg gtggctatgc ggttgttatc ttggcacttg caattgtctt
    45181 tgtcatgttt atctggcatg cgggcacacg gattgttttc aagtatgtga aatctcttaa
    45241 tttgaatgat tacaaggagc agattaaaca acttcgtgac gacgtttgtt ttgacctcta
    45301 ccaaacaaat gttgtctacc tatccaatcg tatgcaggat catatgattg accggtctat
    45361 tttatactcc attttagaca agcggccaaa acgcgctcaa gtttattggt ttgtaaatgt
    45421 ccaggtaact gatgaacctt atacagctaa gtacaaggta gatatgatgg gaacggatta
    45481 tatggttcga gtcaaccttt atcttggttt tagaatgcca cagactgtgc cacgctattt
    45541 gcgcacgatt gttcaggatt tgatggaaag tggccgttta cccaaacaag aacaggaata
    45601 taccatcaca cctggtcgtg atgttggtga ttttcgcttt gttttaatag aagaacgggt
    45661 ctccaatgca cgtcaattga gtaactttga acgctttatt atgcagacta aggcaagcat
    45721 taaacatgtt accgctagtc caatgcgttg gtttgggctt caatattcag aagtaaccct
    45781 tgaagtggtt cccttgattc tatcagatgt cttgaagtta ccaatcaaag aattggtgcc
    45841 tgtggaagac agtgaagcat aaaaaaatca ttgagatgat aagtctcaat gatttttctt
    45901 ttctttttga tgagtagcaa tataggccaa tttctggctt cttgtttttc gttttttaaa
    45961 gagtgcttca gcgctcatcg cagcctcttt gctatcaaac acttcgtaat aaagtaaaga
    46021 aacaggtaac cggtagcgag tatatttagc cccctttcct gcgttatgtg tagctaagcg
    46081 ttttttcaaa tcagttgtat aaccagtgta gagggtttta tcaacacatt ctaacacata
    46141 catataagct tttttagtgg tcatgattag gttttccttt ggaagcagca ccaaaataaa
    46201 tctcaaaaat atgatcagtg tattctccgt tttccttgtg gacaactaaa ggaggtaaaa
    46261 tagtcatccc ttcaatagaa ccgtctttga ttgcttcaat aagtaacata ttagctgatt
    46321 tacctaattt aggatagaca aattgtaccc ttttaggggc aagaccatta gctctaaggc
    46381 tatctataat ttccaagaat cgatctggtc gatgaaccat tgccagacga ccatttgatt
    46441 taagggcatg acgggctacc tgacaaatct cttctaaatt agtggtcact tcatgacgag
    46501 ccagtaaata atgttcagag acgttcttct tagaactttc gtgactttta aagtaagggg
    46561 gattgcagag catcaaatca actcctgaac gtggcacatg gtttaggagg ttttttaaat
    46621 catcacaaat catggttact tggtcttcca gttgattgag ctggatagag cgttgtccca
    46681 tatcagcaag ccgttcttgt aattcgacct caatgatggc tgccttggtt ctagtgctag
    46741 caaataagcc aaccgcccca tttcctgagc acaaatccac aattaaccct ttggaaggca
    46801 tcttggggaa gcgggataag aggacgctat caatagagta actaaagaca tccttgtttt
    46861 gaataatgcc aacatcactt gaaaagagtt gatcaatacg ttcgccttct tttaagattg
    46921 cttttatcat acctgttatt ttaacataaa ataggcttga ggtgaatgtt tgaagagtga
    46981 ttttgacttt cgaaaagtca gctttcatga tacaataagt aagattattc tattaaagga
    47041 gaagcctgtg ttttacgctt acttacgcgg tttagtagtc tttttattat gggttgtcaa
    47101 tggcaatgcc cactaccatc atgaagaaaa gatgttagat gcttctgaga attatattct
    47161 ggtagcaccc catcgaacct tttgggatcc agtttacatg gcatttgcag cacgtcctaa
    47221 gcagtttatt tttatggcta aaaaagagtt gtttgcaaat cgtctctttg cttggtggat
    47281 taaaatgtgt ggtgcttttc ctattgatcg tgacaaaccg agtccagatg ctattcgtta
    47341 ccctgttaat atgctcaaaa agagcaatcg ttctcttctt atgtttccaa gtggaagtag
    47401 gcattcgcaa gaggtcaaag gaggggtcgc tgtgattgct aaattggcta aggtcaagat
    47461 tatgccagca gcctatcagg ggcctatgtc ggtcaaggga ttactagcgg gagaacgggt
    47521 tgacatgact tttgggaatc ccattgacgt ctcagatatt aaacgcatga atgatgaagg
    47581 gattgctgag gtggcaaacc gtattcaggc agagtttgat cggatggatg acgagttggc
    47641 acctttccaa ccaggaaaag caaggaaccc tttaacttat ctctaccgtt taccattagg
    47701 attggttttg gtggtggtat tattattaac catgttattt tcctatattg ctagttttgt
    47761 ttggaatcca gataaacatc gccattaaga acatgagggc taacatgtgc ctgaaataag
    47821 aagtgattga gattagtctt ctattgaaag aagccgctaa tgcttaatca cttttttcat
    47881 ttctaaaaaa gatattcgat gataccctta actcttttaa gcacaaaaaa tgacaatact
    47941 actatacttt tggatatcat ttacgaataa aaagatgagg taaaaaaatg ttagacgaat
    48001 tattaattaa aggaaaatcg tttttgagag agcggtattt tctgccatac ctaattagtc
    48061 ttttattagg actttttcta atactaagct tcagtttttt gttctgggga aataggcaga
    48121 gcaaggcagc cgttcctgct ttgagagaaa tatcgcctgt gaaacagcag gttagtgaag
    48181 aaaaaaagga aattcaagaa gatagcagta ttttagtgga tttaaaaggc gccgtacaaa
    48241 aagagggagt ttataagcta actgctagca gtcgtgtgcg agatgtcatt gaactagctg
    48301 gaggcctaac ttcagaagct gataaacatg ctattaattt tgcagaaaaa ctaactgatg
    48361 agcaagttgt ttatgtacct aaacaaggag aagagatttc tgtgctacca agatcattag
    48421 tttctggtaa aaaagaaact gccagcaagg atcagtcaaa ggttcatatt aataaggcca
    48481 gcttagaaga actacaacat atcccaggta ttggtgctaa aagagctcag gatattattg
    48541 atatgagaga taagttaggt ggttttaaag cgttagaaga tctccgtcag gtttcgggga
    48601 ttggcgaaaa aacgctcgag aaattaaaag atgacatttt cttggactaa gctagtgccc
    48661 ctgtcaaaga ttcaatttgc ttttttaatc ttggttctct tttatcaaat ccatagccct
    48721 agctggttaa cgtttctcct tagccttagt ttgatttgct tacttgtcaa gcgtttgtct
    48781 aaaaaagaat ttctcggtgt ctttgccatc cttagtttct gtgctttgtt tttgctgtat
    48841 cagaaacagc agttgattca aaaactggaa attcagccag tacaaataac ctctgtagca
    48901 ttagttcctg atagtattcg gattaatggt gatcaattag ctgttcttgg acgtcacggg
    48961 aaacattctt atcagttatt ttaccgtttg aaaagccaag cagaagccca attgttcaag
    49021 aaagagcacc gttggttagt gatgcacgca aaagtgacat tggaaaaagc tgaggaagtt
    49081 cgaaatttta aaggctttaa ttatcagact tttttggctt atcaaggaat ttacaggata
    49141 gggaaggtag agcaaataga acagcttgaa gtgatttcac ccgagtcaat atgtgactat
    49201 ttgtcctctt taagacgacg agccattgtt cattgccagc agcattttcc aagacctatg
    49261 tctcactacc tgacgggcct cctttttggt tatttagata agtcgtttgg tgagatgaca
    49321 gattattata gtcagttggg tatcattcat ctctttgctt tatcagggat gcaggttggc
    49381 ttctttctta cttgttttag aagggtgttg ctattactcg cagttccttt ggaatggata
    49441 aaatggatag agcttccttt tgcttgcttt tatgccgcgt tgacaggtta tagtatttct
    49501 gttattcgaa gcctcgtgca gtcgcagttg cgtcatttag gaataaaagg attagacaat
    49561 ctggcttgta cttttttact tgtcttttta tgggatgctc atttcttaat gactgttggt
    49621 ggcgtattaa cgtttagcta tgcttttttg ttaacagtgg ttactgttga agaattatca
    49681 ggagctaaaa gacagttggt acaagtcttg accatttccc taggaatatt accatttttg
    49741 ctcttttatt tttctagttt taaccccatg tcaatggtct taacagggct cttgtcttat
    49801 ctctttgacc tttttatttt gccccttctt tgtctggttt tttgtctatc ccctttggtg
    49861 actgtctcaa tatgtaacca tctttttatt ttattagaaa aagtcattca atttctcgga
    49921 aatactttca atagttccct tgtttttgga agcccgacca gttggcactt gcttatcttg
    49981 gtgattagct tcgctatttt ttatgattat cggcaggtgc gacaacgagt tatcacttgc
    50041 ggtttggtta tagcgttgac gcttttgtct gtcaaatatc ctttgaccaa tgaagtgacc
    50101 tttatcgaca ttggccaggg agatagtatt ttggtgaggg agtggacagg gaaaaatctc
    50161 ttaatagatg taggtgggcg ccctttcttt tcttcaaaag agcactggcg acgaggacat
    50221 cacgtagcta atgctcagaa aaccttaatt ccttacttaa aaagtagagg tattcacaca
    50281 attgaccaac tattagtaac tcacgcagat actgatcata tgggggatat cgaagtggtt
    50341 gccaaagcta ttcgaataaa ggagattttg actagtcaag gcagtttaag ccatccaagc
    50401 tttgttaggc ggctaaggcg tttaaaatgt cacgttaggg ttttagcagc tggagaccag
    50461 ttgcctatta tgggaagtgt cttacaggta ctctaccctt ggcagttagg ggacgggaaa
    50521 aataacgatt ctttggtact ttatggcagg ctcttaaatc gaacttttct tttcacaggt
    50581 gacttggaaa aagaggggga aaacgagatc ataaaacgtt atcctcaact aagagtagat
    50641 tatttaaaag caggtcacca tggatcaaat acctcttcta gtgcagcttt tttagaccat
    50701 attcagccta aagtggcctt tatttctgct ggaaaaaata accgttatca gcatcctcac
    50761 agagaaacct tagcccgttt ggaagacaga caaattactt attaccgaac ggatacgcaa
    50821 ggagctattc gtttgactgg ttggacaagc tggcatcttg aaacggtccg ttagttaaaa
    50881 ttccagcctg acgaagaaac aagcagtcgc tttcctttta ggagaaagat gatataataa
    50941 aagcatgatt gcgatagaaa agattgaaaa actgagtaaa gaaaatttgg gtcttataac
    51001 ccttgtcaca ggagatgaca ttggtcagta tagccagttg aaatcccgct taatggagca
    51061 gattgctttt gataaggatg atttggccta ttcttacttt gatatgtctg aggccgctta
    51121 tcaggatgca gaaatggatc tagtgagcct acccttcttt gctgagcaga aggtggtcat
    51181 ttttgaccat ttgttagata tcacgaccaa taaaaaaagt ttcttaaaag aaaaagacct
    51241 aaaggccttt gaagcctatt tagaaaatcc cttagagact actcgactaa ttatctttgc
    51301 tccaggtaaa ttggatagta agagacggct tgttaagctt ttgaaacgtg atgcccttgt
    51361 tttagaagcc aaccctctga aagaagcaga gctaagaact tattttcaaa aatacagtca
    51421 tcaactgggt ttgggatttg agagtggcgc ttttgaacaa ctgcttttaa aatcaaacga
    51481 tgattttagt caaatcatga aaaacatggc ttttttaaaa gcctataaaa aaacgggaaa
    51541 tattagccta actgatattg agcaagccat tcctaaaagt ttacaagata atatttttga
    51601 cttaaccaga cttgtcctag gaggtaaagt tgatgtggct agagatttga ttcatgattt
    51661 acgcttatca ggggaagatg atattaaatt aattgctatt atgttggggc aatttcgctt
    51721 atatttacag cttgccattc ttgctaaaga aggaaaatca gaacagcagc ttgtaaccag
    51781 tttgtcagag atacttgatc ggcgggttaa tccttaccag gtcaagtatg cgttaaagga
    51841 ttctaggacc ttatctcttg cctttctaac aggagcggtg aaaaccttga ttgagacaga
    51901 ttaccagata aaaacaggac tttatgagaa gagttatcta gttgatattg ctctcttaaa
    51961 aatcatgact cactctcaaa aataggcgtt ggaccgcttt ccaagagata atcaaattag
    52021 ttgaaagctc ttattttttg cgataagata gacgtatcat aaaggaaaag aggacaggaa
    52081 aatggctatt attttaccag aacttccata cgcgtatgat gctcttgaac cacagtttga
    52141 tgcggagaca atgactcttc atcatgataa acaccatgcc acttatgtcg ctaataccaa
    52201 tgcggcttta gaaaaacacc cagagatcgg agaaaatctg gaggaactat tggcagatgt
    52261 cactaagatt ccagaagata ttcgtcaagc cttaattaat aatggtggcg gacacctaaa
    52321 ccatgcgctt ttctgggaac tattatcacc tgaaaaacaa gacgtcactc ctgatgttgc
    52381 acaagccatt gatgatgctt ttgggtcatt tgacgccttt aaagaacaat ttacagcagc
    52441 tgctactgga cgttttggtt caggttgggc atggttagtt gttaataaag aaggacaact
    52501 cgaaatcaca tcaaccgcta accaagatac tccaatttca gaaggcaaaa aaccaattct
    52561 agcgcttgat gtgtgggagc atgcttacta cttgaattat cgtaatgtac gtccaaatta
    52621 tatcaaagct ttctttgaaa tcattaattg gaaaaaagta tcagaacttt accaagccgc
    52681 taaataagaa aagggtttcc aatctcaaaa agagattggt tttttaagtg gttttgtgaa
    52741 taacagtgtg gggacatcat tttttagaaa agataaaaat agtttgtctt tttaatgtaa
    52801 ttccttgatt tttagctaaa tttagctaga atagaatgga taggagattt taggtgttaa
    52861 gaaagaagaa aactaccatt gtagggatta ttttaggaat gatgatagtg accttacttg
    52921 tcggctgttt gactggtaaa aagaaaggct ttcctcctaa agcaggtgag cggcagcaaa
    52981 cggctgactt aaaaatgaaa aaagattaga aaaactgttc aaaaaccgtt acctgaaaaa
    53041 tctaaagagg ctaagcaagc tgttatggat gcagatttag cgcaaatgga tgccttaggg
    53101 ctctattacg atgacaacca tttgagtttg gtcaatacgg tcaaggtgta tttaaaggaa
    53161 tttggcattg gtgaatctca aattgccttt tcctacaaag acctcaattc aggcaggact
    53221 gctgccatga atgaccatca gccaatgatt gcaggaagca cctataagct gccattaaac
    53281 atgctggtgg tagataaggc aaattatcca tgacagagcg ttttgacatt acagaaactg
    53341 attatgaata tgatcaggaa catcatgcct atgttgctca attcaatggt gccatgtcta
    53401 ttccggatat gcaagaatat tccttggtgt actcagaaaa cacgccagct tatgctctag
    53461 cagaacgatt gggtggcatg aataaggctt accaattatt tgaccgctac gggaaggtat
    53521 ccggggctat tacgaccatt gatcgtaatg gcaataaaat caccaccgct tactatttgc
    53581 aaggtttgga ttacttgtgg cagcatcagg ataaatataa ggatatcttg tactacattg
    53641 gtgaatcttt ccctgatttg tattacaaga cttatttgcc tcatgtaaag gtttatcaaa
    53701 aaccgggtta tgtcagagaa gccttaaatg taggtgccat tgtttgcgaa gagtctcctt
    53761 atttgattac tctttattca tctggtttag gtggagcaac acaagcatca gaggaagtca
    53821 atggccttgg ttatgtgcag cttgttcagc taacatatgt aattaatgag tggcatagag
    53881 tcaatcacaa catgatctaa tactcagttg acattagtct gatagtagac atacttactg
    53941 atgaaagtca ctaaaataag tttttgctca ttaaaaaagc ttggcctgtt tcttcaggtc
    54001 aagctttttg agattaatgc tgtgtttttg aggtcttatt gtcacctctt gtttcttgat
    54061 aatggtgaag caattttttg aattttttaa cggcagcgcg tgttagctgc ttagatgatt
    54121 tgccatttct gctgtaaatg ggattgtgct tatccctttg ctgttctaaa tcgggaacta
    54181 gaaaagttgc atcaggacta ttaattgctt tttcaataaa agccggatca aaaagcagaa
    54241 tagggtcaat atcaagcaga cggtgcaagt catcgttttg gttgccataa ttgaaagaag
    54301 aaccattgac aatactattt cgcgttaggt tatcaggatc tagtgctact aaaaattgtc
    54361 cagattctga caaaataaac tctgagtgaa aatgaattaa aatcttatag ttggcttgag
    54421 gaatattgtc tgtatagact gcctgatgat tgccattagc atctaaaata ctagcaacct
    54481 tattatggta tctagaagat tcataaagag cataatcgtc atcttctaga gtagccaaat
    54541 atttggctaa ggcatccgca tcggttttcc ctttattccc ataatgtgag cgaacccact
    54601 ccgtttgctg ggttgatatg aggtaacgga gttgatgaat tttccgatta aaacggctat
    54661 aaggaatgtg ttcgagatta acataataag cctgaatcag ggtagataat tggtcccaaa
    54721 aaggatctgt tggcgatagc tttggactta attgtgccaa tacttgctgt gtttgttttg
    54781 attgtcctgt caactcgtct ggcatcttta aatacgtcaa gagcagttgt aaccgttttt
    54841 gagcttcttg aggcgtctgt gccatctctt cttgttgcca aagcttttga aagtcaggtg
    54901 atcctatcag gcgaatgcga ctataaagtt gttcgaaatg ataagagttt cctctaacag
    54961 cgagttgttc aaggtatcct ttttggatgg ctttatagga ccatccgtcc ttatcgagct
    55021 ttcgaacata atcaagcaag tcgtggcgta atttaatagc ttctgaaagc tggagttctc
    55081 gtaatttagg atagttttgg taaaaaggtg ccactaaatt ttgataaagt ttgtgttctt
    55141 tttcaaaggc aatccgctga tagtctttct gccaaaaact gacgcaaaaa gtcagaaaca
    55201 tcaagaagaa tagccatttt ccaagtaatt tcataggttt ttagttgaca aacatggcat
    55261 cgccaaagct aaagaaacga tacttttcgt caaccgcgtg tctgtaagct tcaaggacaa
    55321 agtcacgccc tgcaaaggct gatactagca tcacaagggt tgattttgga agatggaaat
    55381 tagttgaaaa agcatcaact accttgaatt ggtagcctgg tttaataaaa atattggtcc
    55441 aacctgaatc agcttgaatg tccccttgga acttacctcc gatggtttcc aacgtacgaa
    55501 tagaggtggt tcctacagcg acaacacgac cgccagcttg tttgacgtct cttagggttt
    55561 gcgctgcttc ttctgagagg gaataaaatt ccgaatgcat atcatgctca tcgaggttat
    55621 ccacggaaac aggtctaaaa gtcccaagcc ctacatgtag ggtcaaataa accaaatgta
    55681 cccctttggc ttcgattttt ttgagcaagt ctgttgtgaa gtggagacca gcagttgggg
    55741 cggccgcaga gccattttct ttagcataaa cggtttggta tcgctcagca tcctctaatt
    55801 tttcgtgaat ataaggaggc aatggcattt cgccgaggct ctctaaaact tctaagaaaa
    55861 tcccatcata gctgaattca acaattcgtc caccatgttc taattcgtcg ataatggtgg
    55921 ctttgagacg cccatctcca aaattgactt ggctaccaac cttgagccgt ttggctggct
    55981 tggctaaaac ttcccattga tctccttgtg tatttttaag caaaagcaat tccacatgac
    56041 catgagtatc gggtttttct ccataaagtc tggcaggcaa gacacgggtg ttattcatga
    56101 cgagggcatc ccctggattt aattgatcaa tgatatggtc aaaatggcta tcaaccatag
    56161 ttttttgacg gtgatcaata atcaatagtt ttgaactgtc tcttttttcg agtggggttt
    56221 gggcaatcaa ttcttctggt aactcaaaat caaaatcgtt agtattcatt gggactcctt
    56281 taaagccact agtaaatctc ttcctagtaa ctctttcatg taagataaat cttttctatt
    56341 atatcacttg tcgtctaacc cgtcatcttt aatctttcat cagaaagtaa aggataggct
    56401 tgacaaaaat tggtatatac catataataa aaacacgaaa ggtctatacc aattagaaaa
    56461 ggagaatgac atgaaaatta ttcgggtcca agatcagatt gaaggtggga aaatcgcttt
    56521 taccttattg aaagatagct tagctaaggg agctaagacg ttaggattag caacaggaag
    56581 ttcaccaatt agtttttacc aagaaatggt caaaagtcct ttggattttt cggacttaac
    56641 aagtattaac ttggatgaat atgtcggtct ttcagtagag agcgatcaga gttatgatta
    56701 ttttatgcgt caaaaccttt ttaatgcgaa gccttttaaa aagaattact tgcccaatgg
    56761 tttagcaact gatatcgaag ctgaagctaa acgatatgac caaatcattg cagggcatcc
    56821 gattgatttt caagtgcttg ggattgggcg taatgggcac attggtttta acgaacctgg
    56881 gacctctttt gaagaagaaa cgcatgtagt ggatcttcag gaatcaacta tcaaagccaa
    56941 tagtcgtttc tttacaagta ttgaggatgt gccaaaacaa gctatctcta tgggaattgc
    57001 ctccatcatg aagtccaaaa tgattgttct tcttgccttt ggacaagaaa aagctgatgc
    57061 gatcaaggga atggtttttg gaccaatcac agaagacctc ccagcaagta ttctccaaaa
    57121 acatgatcat gttatcgtca ttgtggatga agctgctgcg tcacaattag attaacctaa
    57181 ccagtcgtta aaccttgtaa gaacaaaaat gaggagatag tcaacctcat ttaagaagaa
    57241 gtcttgtaac ttctatttct tttgcaaggg taaatttatg ttacactact aatatgcgtt
    57301 tagataaatt attagaaggt acaaaagttg gctctagaag tcaagtgaag aaactgatta
    57361 aggctcaagg ggtttgggtt gatcacatgc ctgccagaaa tggtcgacaa aatgttgatc
    57421 ctggcttgca actgattgag gtgactggac agcgagtgac gcatcctaaa cattcctaca
    57481 ttatcctcaa caagccatca ggggtggtct cagctaagaa agatactaac tatttaaccg
    57541 ttattgatca gcttgctgaa gaagataaaa gtccagactt gtatcctgtt ggcagattgg
    57601 atagagatac cgaagggttg gttttactca ctgataatgg tcccctaggg tttcggatgt
    57661 tgcaccctat tcatcatgtt tctaaaacgt atcttgtaac cgttaatggc ttgttagcag
    57721 aagatgcctc agattttttt gcagctggta tttgtttccc aacaggtgaa caatgtcaac
    57781 cagctcaatt gactattctt aaagccgaca cagaccagtc acaggctagt ttgaccattt
    57841 cagaaggaaa atttcaccaa gtgaaaaaaa tgtttttgac ctatggtttg aaagtgacct
    57901 ctctgaaacg gacgcacttt gcaggattgg aactcggtca tttagccagc ggagaatatc
    57961 gttatctaac agcgtcagaa aggcaactca ttaaaacgta tctagactga gcaaaacatg
    58021 ctttcaaaaa gaagctattc ttgcttaatc aataaagtgt ccattgacta ttatagtcat
    58081 gatgagtccg acttttgcta tgtttagtca tcttgttgtt acaaaatcag gtaagcaaaa
    58141 aaattcaatt cccactgtct ccttaacctg aagcgttata aaaacccttg tgttaaactc
    58201 tcataaattg gaaatgggag tgttttttga tggctaaaaa aactaaagct acttaaagtt
    58261 tggtacttag ttttcgaata ataaggtgga agatctaata ggaggtgaca aaaatattaa
    58321 ccgctttaga tggtaagaat cagctcattt cgctagtaac tcagcctatt tcaacgaaac
    58381 cgcctttttg ttgcccagct tgcaaatctc ctgttcgctt gcgtcagggg acaattagac
    58441 gacctcattt tgcccatgta caactagctc attgtcaatt ccaagcagaa aatgaatctg
    58501 aggaacattt gacgttgaag gcaaaacttt acaccagttt agtccgaaca gaagctgtct
    58561 gtattgaaaa atatcttcct gagctgcaac agatagcaga cctttgggtg aatgataagt
    58621 tagccttaga aattcagtgc agtcccttgc cagttgagcg tctaaagaaa cgcaccaaag
    58681 cctatcaaga aaaggggtat ccggtacgat ggttactggg aagaaaattg tggcttaaca
    58741 ctcacttaac tgctttgcaa aaacagtttc tttacttttc atctagtttg ggctttcatt
    58801 tgtgggagct ggatgcagct gcaaatctgc tacgtctcaa atatttaatc catgaagact
    58861 tatttggtaa ggtaagttac ctaaccaaaa ccatttcttt agaccacaat attatggaaa
    58921 tgtttcgatt accctatcag caagaaatcc tttattctta tcagaaaaaa atgactgtga
    58981 acctatctaa aagaatacaa agagctcttt tagctagaca cccgaaatgg ctacgacgac
    59041 aagaaaaggc ttacttatca ggctataatt tacttatgtt aacaactgat gctttttatc
    59101 ctcaatggag accagttcaa tcttccagcg gtttttgtca aattgaagga aatcttcgcc
    59161 cttattatga aagttttaaa gtctattata aaaaagaaaa ggataagaag gtgcaaacgc
    59221 ttttttcacc aaaatattat gttaaaatgg atagtaatag aaaatgagaa ggagatgtta
    59281 tgactgataa tcgcagccat ttagaagaaa aatacacttg ggatttgagc actatttttg
    59341 caacagataa ggattgggaa gctgaagtca gtgacttagc tactgaaatt gacgcggcta
    59401 aggatttttc aggtcattta ctagattcta gcgctaatct gttgacaatt acagatactt
    59461 atttagagtt agctcgtcgt gttgagaaag tctatgttta tgctcacatg aaaaatgacc
    59521 aagacactac tgttgccaag tatcaagaat accaagctaa ggcttcaggc ttgtatgcaa
    59581 agtttagcga agtgttttca ttctatgacc cagaagttat ggcattgagt caggaagatt
    59641 accaagcttt tcttgcggaa acacctgatt taaaaaggta tcatcatttc tttgacaaat
    59701 tgttccagac acgtgaccat gtgctcagcc aggctgaaga ggaattatta gcaggagccc
    59761 aagaaatttt caacggcgct gaagaaacct ttagtattct tgataacgct gatattgtct
    59821 ttccagtagt taaaaatgat aagggggaag atgttgaatt aacccatggt aacttcatta
    59881 gcctaatgga atctaaggac cgtagcgttc gccaagctgc ttatgaagcc atgtatagta
    59941 cttatgaaca gttccaacat acttacgcta aaaccttgca aaccaatgtg aaagtgcaaa
    60001 actataaggc gcgtgttcat aaatacgatt cagcacgtca agctgccatg gctgctaact
    60061 ttattccaga agctgtttac gacactttat tagaaacggt taacaaacat ttaccactct
    60121 tacaccgtta tttgaaattg cgtcaagagg ttcttggttt agacgatttg aagatgtatg
    60181 atgtttacac accattatct gaaacagatc tagccattgg ttatgatgag gctttggaaa
    60241 aggctgagaa ggttttagct gtttttggta aagattacgc agatcgtgtg catcgtgctt
    60301 ttaccgaacg ttggattgat gttcatgtca ataaagggaa gcgttctgga gcttattcgg
    60361 gtggttccta tgataccaat gcctttatgc tattaaactg gcaagatacc ttagacaatc
    60421 tatacacttt agttcatgaa actgggcaca gtttgcattc tactttcact cgtgaaacac
    60481 agccttatgt gtatggagat tacagtattt tcttggcaga aattgcatcc acaacgaatg
    60541 aaaatattat gacagaagct ctcttaaatg aggttcaaga tgagaaagaa cgctttgcca
    60601 ttttaaacca ttacctcgat ggtttccgtg gaacagtttt ccgtcaaacc caatttgctg
    60661 agtttgaaca tgccattcat caagctgatc aaaaaggaga agtgttaaca agtgaatacc
    60721 ttaaccagct atacgctgat ttaaatgaaa aatattatgg tttaagtaaa aaagataatc
    60781 attttattca gtatgaatgg gcacgtattc ctcatttcta ttacaattat tatgtttacc
    60841 aatatgcaac aggttttgca gctgcaagct atttagcgga taagattgtg catggcacac
    60901 aagacgatat cgatcattac ttggcctacc tcaagtcagg aaattctgat tacccacttg
    60961 aagtgattgc taaagcaggc gttgatatga caaaaggtga ttatttggaa gccgccttta
    61021 aagtctttga cgaacgtcta acagaacttg aagtactcgt gtccaaaggc atacacaaaa
    61081 gctaaaatca caaaagtcaa ggacaaaaag agtttaggat tgatagaaat ggagattcac
    61141 agttggtgtg aatctccatt tctatcggtc attttctttt tatattttag tggattatca
    61201 gtcctgaaaa gcaggcttca ttgaccttag tttgacggtt tcttttttgt cttttttatt
    61261 gtgtggtcac tcacttaaat aaaactaata gttgagtaag gcgcttcaga ttaacgctat
    61321 tctattgctt tagagataac gttcgcataa aacatatttt tattatattt gacattttat
    61381 aaaagagata atagaattaa aatactaaca tttatgtttt ttaaaaaata aacgttcgta
    61441 taataaaaaa ggagtttttc atggaaaaaa caaaacgtta tatcattgca acagcaggga
    61501 tacttctcca cttaatgttg ggatcgacct atgcttggag tgtttatcgt aatccaattt
    61561 tacaagagac cggttgggac caagctcctg tcgcttttgc cttttcttta gctatttttt
    61621 gtttaggttt atctgctgct tttatgggaa atttagttga acaatatggt cctcgtctaa
    61681 ccggaacggt gtcagctatt ctttatgctt ctggaaatat gctgactggt ttagcgattg
    61741 atcgtaaaga aatttggctt ttatatattg gttatggtgt tattggtggc ttaggacttg
    61801 gagcgggata tatcacaccg atatcaacta ttataaaatg gtttccagat aaaagaggca
    61861 tggcaacagg gtttgctatt atggggtttg gttttgcgtc tttattaaca agtccgattg
    61921 cccagtggct aattgaaaca gaaggtttgg tagcaacatt ttacctttta gggctgattt
    61981 atctaatagt tatgcttttt gcttctcaat tgataataaa accaactgct gctgagatag
    62041 ctatactaga taaaaaaaga ttacaaaata acagttattt aatcgaagga atgaccgcta
    62101 aagaagcact taaaacaaaa tcattttatt gtctttgggt aattttattt atcaatatca
    62161 catgtggctt aggacttatc tcagttgttg cgccaatggc acaagatcta actggtatga
    62221 gtccagaaat gtcagcaatt gtggttggtg ctatggggat ttttaacggt ttcggtcgtt
    62281 tagtgtgggc tagtttatca gactatattg gacgccgtgt gacagtaata cttttatttt
    62341 tggtaagcat aattatgact attagtttaa tatttgcaca tagttcccta attttcatga
    62401 taagcattgc cacccttatg acttgttatg gtgcgggttt ttctctcatt ccgccttatt
    62461 taagtgatct ttttggggca aaggaactgg ctacattaca tgggtatata ttgacagctt
    62521 gggcaatagc tgcattaact ggacctatgt tattatctat cactgttgaa tggactcata
    62581 attatcttct taccttatgt gtttttattg tcttatacat tttaggatta atggtagtat
    62641 taaggttgaa gaaatgatgt cctataaaaa atttaaaagt aaaccctagc aaagcctagg
    62701 gtttttttat ataattaggt ttatggttaa atcatacagt aaaacagcaa atcataatat
    62761 gcgtcgtcct gttgtcaaag aagagcttgt tcattatatg cgaacacggc agaagcaaac
    62821 cacaggtttt ttagcagagt tagagcaatt cgcaagacaa gaaaacattc ctattattca
    62881 gcctgaggtc gtggcttatt tcaggttttt attgcaaagc ttacagccaa aacacatttt
    62941 ggagattggc acagccattg gtttttctgc gcttttaatg gctgaaaatg caccagatgc
    63001 gacgattgtg actattgatc gtaatcgaga gatgatcgat tttgctaaag caaattttgc
    63061 taagtacgac agtcgccaac agatccgcct gttagaagga gatgcagcag atatattatc
    63121 tacacttgaa ggaaattttg actttgtgtt tatggattcc gccaaatcca aatatattgt
    63181 ctttctacca gagattttaa gactgctaaa agtcggtggt gtagtcattt tggatgatgt
    63241 ctttcaagga ggtgacatta caaaaccgat cgaagacatt cgacgaggtc agcgtactat
    63301 ttatcgtggc ttacagtctt tgtttgatgc caccttaact catccaaacc taacaactag
    63361 cctggtccct cttagtgatg gattattaat gattcgtaaa aatcaagcag atattgtatt
    63421 accagattaa gtaacattga taaatttatg ttataataaa ttagttaaag ataaaaagga
    63481 gctcagaagt aaatgaaaaa ctcaaataaa ctcattgcta gtgttgtgac attggcctca
    63541 gtgatggctt tagcagcttg tcaatcaact aatgacaata ctaaggttat ttcgatgaaa
    63601 ggtgatacaa ttagcgttag tgatttttac aatgaaacaa aaaacacaga agtatcgcaa
    63661 aaagcgatgc taaatctggt aattagtcgt gtttttgaag ctcaatatgg tgataaggtt
    63721 tcaaaaaaag aagttgaaaa ggcgtatcat aaaacagctg aacagtatgg cgcttcattc
    63781 tctgctgctt tggcacaatc aagcttgaca cctgagactt ttaagcgtca gatccgctct
    63841 tcaaaattag tagaatatgc ggttaaagaa gcagctaaaa aagaattgac aacacaagaa
    63901 tataagaaag catatgaatc ttatactcca acaatggcag tcgaaatgat tactttagat
    63961 aatgaagaga cagctaaatc agtcttagag gaactaaaag ccgaaggcgc agactttaca
    64021 gctattgcta aagaaaaaac aacaacacct gagaaaaaag tgacctataa atttgattca
    64081 ggcgcgacaa atgtaccgac tgatgtcgta aaagcggctt caagtttgaa tgagggtggc
    64141 atatcagacg ttatctcggt tttagatcca acttcttatc aaaagaagtt ttacattgtt
    64201 aaggtgacta aaaaagcaga aaaaaaatca gattggcaag aatataagaa acgtttgaaa
    64261 gctatcatta tagctgaaaa atcaaaagat atgaatttcc aaaacaaggt tattgcaaat
    64321 gcattggata aagctaatgt aaaaattaaa gacaaagctt ttgctaatat tttggcgcaa
    64381 tatgcaaatc ttggtcaaaa aactaaagct gcaagtgaaa gttcaacaac cagcgaatca
    64441 tcaaaagctg cagaagagaa cccatcagaa tcagagcaaa cacagacatc atcagctgaa
    64501 gaaccaactg agactgaggc tcagacgcaa gagccagctg cacaataagt aggattttat
    64561 tgactaatca gttaaaaaaa cttataataa tttagttaag aattgtttat caaggattag
    64621 atacagaaaa gtgacggttg ctgcgagtca ttgataatta gataaacatg ctacttaact
    64681 ttacaacaat tgatatcgta aattaagaat gacaagttca ttgaatagag gtggtaccgc
    64741 ggttttcgcc ctctgtgaga tggacttgtt ttgtattgag gactatttga aatcaacgaa
    64801 aggaatagag tagactaggc tgccaggctt atagctgatc tcctagcaag aaagacgagc
    64861 tgtgtcaact ggtgtgatta cattactagc ttcagcataa cactgttttt cttactcttt
    64921 gtatctaaat ttttatgaaa gaattatcgt ctgcacaaat ccgccaaatg tggttggatt
    64981 tctggaaatc taaaggacat tgcgttgagc cttcagctaa cttggttcct gtgaatgacc
    65041 caacgcttct ttggatcaac tcaggtgttg caaccttgaa aaaatacttt gatggttcag
    65101 tgattccaga aaatccacgt attaccaatg cacaaaaatc aattcgtact aatgatattg
    65161 aaaatgttgg taaaacagca cgtcaccata ctatgtttga aatgcttggt aacttctcaa
    65221 ttggagacta tttccgtgat gaagctattg agtggggatt tgaactcttg acaagtccag
    65281 actggtttga tttccctaaa gataagctct acatgactta ttacccagat gacaaggatt
    65341 catataaccg ttggattgct tgtggcgttg aaccaagtca cttggtgccg atcgaggata
    65401 acttctggga aatcggtgct ggtccttcag gtccagatac ggagattttc ttcgatcgtg
    65461 gtgaagattt cgatccagaa aatatcggac ttcgcctctt ggctgaagat atcgaaaacg
    65521 atcgttacat cgaaatctgg aacatcgttc tctcacaatt caatgctgac ccagccgtac
    65581 cacgttcaga atacaaagaa ttaccaaaca aaaacattga tacaggtgct ggtcttgaac
    65641 gtcttgcagc tgttatgcaa ggggcaaaaa caaactttga aactgacctc ttcatgccaa
    65701 tcatccgtga agtagagaag ttgtcaggta aaacttataa tccagatggc gacaacatga
    65761 gtttcaaggt tatcgctgac cacatccgtg cgctttcatt tgctatcggt gatggtgcgc
    65821 ttcctggaaa tgaaggtcgt ggttacgttc ttcgtcgtct tctccgtcgt gctgttatgc
    65881 atggtcgtcg tcttggcatc aacgaaactt tcctttacaa attggttccg actgttggac
    65941 aaatcatgga aagctactac ccagaagtgc ttgaaaaaca tgattttatc gagaaaatcg
    66001 ttaaacgtga ggaagaaaca tttgctcgta ctatcgatgc aggtagcggt cacttagatt
    66061 cattgcttgc gcagcttaag gctgaaggta aggatactct tgaaggtaaa gatatcttca
    66121 aactttatga tacttatgga ttcccggttg aattgacaga ggaattggca gaagatgcag
    66181 gctacaagat tgatcacgaa ggctttaagt cagccatgaa agaacaacaa gaccgtgcgc
    66241 gtgcagctgt tgttaagggt ggttcaatgg ggatgcaaaa tgaaacccta gctggtattg
    66301 ttgaagaatc acgattcgaa tacgacacat atagtcttga atcaagtctt tcagtcatca
    66361 tcgctgataa tgaacgtacc gaagctgttt cagaaggtca agctcttctt gtctttgctc
    66421 aaacaccatt ctatgctgaa atgggtggac aggttgctga cacaggtaga atcaaaaatg
    66481 ataagggtga cacagttgct gaggttgttg atgttcaaaa agcaccaaat ggtcaacctc
    66541 tacacactgt aaacgttttg gcatcacttt cagttggaac aaactacaca cttgaaatca
    66601 acaaagagcg tcgtttggct gttgagaaaa accacacagc aactcacttg ctccatgcag
    66661 cccttcacaa tgttatcggt gaacacgcaa ctcaggctgg ttcattgaac gaagaagaat
    66721 tcttgcgctt tgattttact cactttgaag cagtaagcaa tgaggaactt cgtcacattg
    66781 aacaagaagt taatgagcaa atttggaacg ctcttacgat cacaacgact gaaactgacg
    66841 ttgaaaccgc aaaagagatg ggagcaatgg cactttttgg tgagaaatat ggtaaagtgg
    66901 ttcgtgtggt tcaaattggt aattattctg ttgaactttg tggtggaact cacttaaata
    66961 attcttcaga aatcggtctc ttcaagattg tcaaagaaga aggtattggt tcaggcactc
    67021 gtcgtattat tgcagttact ggtagacaag cttttgaagc ttatcgtaac caagaggatg
    67081 ccctaaaaga gatcgctgct actgtaaaag ctccgcaatt gaaagatgca gcagctaaag
    67141 tacaagctct tagcgactca cttcgtgatc ttcaaaaaga aaatgcagag cttaaagaaa
    67201 aagcagcagc tgcagcagct ggtgatgtct ttaaagatgt tcaagaagct aagggcgtgc
    67261 gcttcattgc tagtcaagtt gatgttgcag atgcaggggc acttcgtaca tttgctgata
    67321 actggaaaca aaaagactac tctgatgtgc ttgttctcgt agcagctatt ggtgagaagg
    67381 ttaatgtcct tgttgcaagc aaaactaaag atgtccacgc tggtaacatg atcaaagaat
    67441 tggcaccaat tgtagcaggt cgtggtggag gtaaaccaga catggctatg gcaggtggta
    67501 gcgatgcaag taaaattgca gagctgctag cagcagttgc tgaaacagtg taaatccata
    67561 aaaaccaagt tatttagaac ttggtttttt atatttgaaa tgactaccat cataaccttt
    67621 ttgactatta tcgttgtcaa aaagaaaaag atatgttaca attatagtaa caaatagaca
    67681 tgaggaggtg ttagagaaaa tgacacatgg tatcaaactt aagaggcaga tttgcttatg
    67741 ggcaagtatc ctgactcttt tcattttaag ttatcatctt atcagggtaa tctggtttgg
    67801 agtacccttg agttatctgg ttttgggctt tacaggacta gccttgctgt tgaatacttt
    67861 ttcttatttt agaaagtaag ttataagtta aagaagaatg aaggagatgg caatggaggt
    67921 tattcttaaa aatcggctca aagaattacg agccagagat ggaattaatc aaacggaaat
    67981 ggctaaactg gcaggagtat cgaggcagac catcagtctc attgagcgta acgagtacat
    68041 cccatcagtt ataattgcca tgaaaattgc aaaggtattt caggaaccag ttgaagaggt
    68101 gtttcgtcta gtggaggtcg aggaatgaaa aagaagaaga atagtttttt acgcttgtta
    68161 aaaatgttat tgctatcaag tttggcggga gggattatag gtggcatggt tggagccttt
    68221 ttaggttacc atggagagag attagatcag ctcacgttcc taaaagatga tgtgattaac
    68281 ctgattatct tattaaatcg tctagtagtt gtcacaggcc tcacactttc ctttgttttt
    68341 ttaacgcaac tgaaaaaaga gacagctgtt tataacacaa ttgaagagga tgattattct
    68401 gaaaatggtt accgtcagct gaataaaaag catgcttata ccatgttatt gattgccgta
    68461 gcttctattt tatccatgtg caatgtgcta cttggtctga ccttaactaa tgattcacag
    68521 catgccatgc tggccattcc gcttctggat atccttttat tattaatggt cattcctttt
    68581 caagctttag ccatgaaacg ttacaatgcc attcagggaa ccgatgttcc ttattttcca
    68641 aatttgaaag aattaaaaca taatatcatg gctctagatg aggcagagtt acaagcctat
    68701 cataagacat cgtttgaaag tgtgttgtca ttaaatggag tcatcatccc ttccttgtat
    68761 gtgattttat tttttgtcta tctattcact ggtcaagtgg agttaacagc catcttagtt
    68821 ttggtgttaa ttcagctcta tttactcgtt aagagtgcaa caatgaccag acaattttac
    68881 cgttagccta aaaggagact gtttatgaaa ggattcatta attatttaaa aatagcagtg
    68941 ctcattattc tggctatggt tttcaatgtc cttccgatga ttttattgca aaagcaacac
    69001 gatattccta tggtgcttaa ttggggaatt ggtattttct acttggttat tgttggaagt
    69061 gtccttattg tattatggcg tctttatcaa gctaagcaag acacccctat taaacagcaa
    69121 aaaatgagat tggttgactg gggttattta gcattatttt ggttaatcat ccgtgtgata
    69181 gctattgcag gtacccttgt taaccagcta tggtctggtc aacaagtgag tgctaatgat
    69241 gctgcaatac ataccttagc tagacttatc aaaggtggtt tcccgcttta tactgcccta
    69301 tttgtacttg tgatagcttt tatcgctcct attatggaag aactagtctt tagaggattt
    69361 cctatgattg atctcttcaa aggaaaatca cttaaggtgg caggtttagt gacctctctt
    69421 gtttttgctt taccacatgc caccaatagt gttgaattta tcatgtacag ctgtatgggc
    69481 atttttctct ttgttgccta tcaaagacga ggaaacttaa aagacgctat cttgttacat
    69541 atttttaata acttgattga agtcattttg ttaatgtcaa taggcttagg agtcatataa
    69601 taaaaagctt cgaatggatg aactgcaccc caaaagttgg acaaaaaaat ctaacatttg
    69661 ggttggaatt gtcaagaaaa aatagacata aagttaagct aatgagtaac ta
//
LOCUS       DAUDYX010000003        64487 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.003, whole genome shotgun sequence.
ACCESSION   DAUDYX010000003 DAUDYX010000000
VERSION     DAUDYX010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 64487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64487
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.003"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..90
                     /locus_tag="VHD02_000164"
     CDS             <1..90
                     /locus_tag="VHD02_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEQ5496804.1"
                     /translation="FDYVLPHEKVKNGEVVTDEKVEEVLMELE"
     gene            335..871
                     /gene="rimP"
                     /locus_tag="VHD02_000165"
     CDS             335..871
                     /gene="rimP"
                     /locus_tag="VHD02_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEQ5496805.1"
                     /translation="MDSQGPIILEKSIKIEEVIKIANTSIIDIVTKTVTPEIKAPYEL
                     VDVEYDKMGSDYILSILVDKEGGITVEDTSDLTNIISPLLDTIDPDPFPNQYMLEVSS
                     PGLERPLKTADSLKAAVGSYINVSLYQAIDKVKVFQGDLLAFDGETLTIDYLDKTRHK
                     IVNIPYQAVAKVRMAVKL"
     gene            1046..2203
                     /gene="nusA"
                     /locus_tag="VHD02_000166"
     CDS             1046..2203
                     /gene="nusA"
                     /locus_tag="VHD02_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEQ5496806.1"
                     /translation="MSKEMLEAFRILEEEKHIDKADIIDAVTESLKSAYKRRYGQSES
                     CVIEFNEKTADFQVFTVREVVEEVFDSRLEISLKDALAISSAYELGDKIRFEESVNEF
                     GRVAAQSAKQTIMEKMRRQMREVMFNEYKEHEGEIMTGTVERFDQRFIYVNLGSLEAQ
                     LSHQDQIPGETFKSHDRIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDG
                     TVEIMSVSREAGDRTKVAVRSHNPNVDAIGTIVGRGGSNIKKVISKFHPKRVDAKTGL
                     EIPVEENIDVIQWVDDPAEFIYNAIAPAEVDMVLFDDEDLKRATVVVPDSKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEYDRLEAEKEAATAVEEPVVDDVMTAEEV"
     gene            2219..2515
                     /locus_tag="VHD02_000167"
     CDS             2219..2515
                     /locus_tag="VHD02_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEQ5496807.1"
                     /translation="MSKVKKIPLRKSLVSGEIIAKRDLLRIVKTKDGQVFIDPTGKQN
                     GRGAYIKLDNQEALMAKKKQVFNRSFSMDIPESFYDDLIAYVDHKIKRRELGLD"
     gene            2508..2810
                     /locus_tag="VHD02_000168"
     CDS             2508..2810
                     /locus_tag="VHD02_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEQ5496808.1"
                     /translation="MTNLERLSSLIGLAQRAGKVISGEELVVKAIQHQQVILVFLAND
                     AGPNVTKKVTDKSNYYNVEVSTVLNALELSAALGKPRKVAAIADAGFSKKMRTLME"
     gene            2830..5691
                     /gene="infB"
                     /locus_tag="VHD02_000169"
     CDS             2830..5691
                     /gene="infB"
                     /locus_tag="VHD02_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEQ5496809.1"
                     /translation="MSKKRLHEIAKEIGKSSKEVVEHAKYLGLDVKSHASSVEEADAK
                     KIISSFSKASKPDVTASQTVKPKEVAQPSVTVVKETGSEHVEKTQVSKPKSRNFKAER
                     EARAKEQAARKQANGSSHRSQERRGGYRQPNNHQTNEQGDKRITHRSQGDTNDKRIER
                     KASNVSPRHDNHQLVGDRNRSFAKENHKNGRFTNQKKQGRQEPQSKSPKIDFKARAAA
                     LKAEQNAEYSRQSETRFRAQQEAKRLAELARQEAKEAALKAQAEEMSHREAALKSIEE
                     AETKLKSSNISAKSTADNRRKKQARPEKNRELTHHSQEGQKKNKKSWNSQNQVRNQKN
                     SNWNKNKKTKKGKNVKNTNTAPKPVTERKFHELPKEFEYTEGMTVAEIAKRIKREPAE
                     IVKKLFMMGVMATQNQSLDGDTIELLMVDYGIEAKAKVEVDDADIERFFEDENYLNPE
                     NIVERAPVVTIMGHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIEEAGKKITFL
                     DTPGHAAFTSMRARGASVTDITILIVAADDGVMPQTIEAINHSKAAGVPIIVAINKID
                     KPGANPERVIAELAEYGIISTAWGGECEFVEISAKFNKNIDELLETVLLVAEVEELKA
                     DPTVRAIGTVIEARLDKGKGAIATLLVQQGTLHVQDPIVVGNTFGRVRAMVNDLGRRV
                     KSAEPSTPVSITGLNETPMAGDHFAVYADEKAARAAGEERSKRALLKQRQNTQRVSLD
                     NLFDTLKAGEIKTVNVIIKADVQGSVEALAASLVKIEVEGVRVNVVHSAVGAINESDV
                     TLAEASNAVIIGFNVRPTPQARQQADTDDVEIRLHSIIYKVIEEVEEAMKGKLDPVYQ
                     EKILGEAIIRETFKVSKVGTIGGFMVINGKVTRDSSVRVIRDSVVIFDGKLASLKHYK
                     DDVKEVGNAQEGGLMIENFNDLKVDDTIEAYIMEEIVRK"
     gene            5897..6247
                     /gene="rbfA"
                     /locus_tag="VHD02_000170"
     CDS             5897..6247
                     /gene="rbfA"
                     /locus_tag="VHD02_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEQ5496810.1"
                     /translation="MANHRIDRVGMEIKREVNDILQKKVRDPRVQGVTITEVQMQGDL
                     SLAKVYYTIMSDLASDNQKAQTGLEKATGTIKRELGKQLTMYKIPDLVFEKDNSIAYG
                     NKIDQLLRELDNKS"
     gene            complement(6378..7367)
                     /locus_tag="VHD02_000171"
     CDS             complement(6378..7367)
                     /locus_tag="VHD02_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEQ5496811.1"
                     /translation="MKHPIRKTLVTLGLLLTLCLPTPVAASSRSWKSWFIEQYFWLKR
                     DKSYYSKQDDPSFQRYLDACREQSDKPYQLDTNLVNGPLVQENLYGMQVYSWNDNGKP
                     DQKTIIYLAGGSYLNNPTTYHINMLKTLSTSLDAKIVLPIYPKAPRYTYNYTMPKLVN
                     LYQHYYHKNQNIFLMGDSAGGGLALGLAHALHNESVPQPKQLVLLSPWLDVTMSHPEI
                     PNYEDADPILSSWGLKRVGELWAYSADNTNHIYVSPKNGPITYLPPITLFTGTREIFY
                     PDIRDYAAKLKAANHNITFITQEGMNHVYPIYPIEEAKTAQYQIIDAINKTPQ"
     gene            7539..7973
                     /locus_tag="VHD02_000172"
     CDS             7539..7973
                     /locus_tag="VHD02_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEQ5496812.1"
                     /translation="MQQISAAEWEVMRVVWASGDIKSSDIITILRKKYQWSDSTIKTL
                     IGRLVKKNLLTSYREGRAYIYQALLDETLVQKEALATVLDGICQRQHTRLLLERLYDL
                     PMTLEEIEAFQELLEVKKENAVLEVPCNCLPGQCHCQKKETY"
     gene            7973..10204
                     /locus_tag="VHD02_000173"
     CDS             7973..10204
                     /locus_tag="VHD02_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEQ5496813.1"
                     /translation="MAKEIFLVEGMSCASCALTIEKTVNQLPEVEQAVVNLATEKLTV
                     TYQDDDLETAKVIQAVKAAGYGAQVFDEHAKHMQTGRRDEDLKVIWSQVLWSALFTIP
                     ILYLAMGHMVGLWLPSFLQPDRYPFVYSFSQLLLTIPVLVLNRHYYHNGFKALFKGHP
                     NMDSLVALATSFAFAYSLYGVVEISLGQAHFVHSLYFESVVVILTLIGLGKYFESRSK
                     GRTSQAIQKLLSLKATDVRVWRNSQWQLLPLEEVSYDDLVLVQPGEKVSIDGIIVEGH
                     SSLDESFLTGESLPVEKGEKDNVFAGSLNGPGALTIKPDKLGNDTLLAQIIQLVENAQ
                     ENKAPIAAIADRVSGVFVPVVLVLALLTGVFWLVFMQESLRFSLTTAIAVLVIACPCA
                     LGLATPTAIMVGTGRAAENGILFKGGDILEQAHQVDTVVFDKTGTVTEGKPSLQKLLI
                     FSGDDHLILQEAASLEAYSQHPLGEAIVRAAQEAGYDSLPVEQFESLTGLGVTGQLEG
                     RRLAIGNATLMAILGIDLSCVQSVCAKASGKGQTLVYYAKEGQLRALFSIADAVKEDS
                     QATVEALQQLGIHTIMLTGDHDATAKAIASQVGITDVISQVLPDQKAGVIADLRSQGK
                     KVAMVGDGINDAPALAVADIGIAMGSGTDIAIESADVILMRSDMLDLVKVMSLSRATM
                     RIVKENLFWAFIYNVLMIPVAMGLLHLFGGPLLNPMLAGFAMSFSSVSVVLNALRLKG
                     KTI"
     gene            10218..10421
                     /locus_tag="VHD02_000174"
     CDS             10218..10421
                     /locus_tag="VHD02_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEQ5496814.1"
                     /translation="MEKHYQVTGMTCDGCVRTVTEKLSAVPGVQSVQVNLEKGQAKVT
                     GRPLTFLLKRALKDTKFELHQSS"
     gene            complement(10621..11391)
                     /locus_tag="VHD02_000175"
     CDS             complement(10621..11391)
                     /locus_tag="VHD02_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ5496815.1"
                     /translation="MLKRERLLKITEIVNKQGIVTVNDIIQTLNVSDMTVRRDLDELE
                     KAGKLIRIHGGAQSITMPNKKERSNIEKQTVQTKEKWELASYATQLVNDGETIFIGPG
                     TTLECFAEQLKNRQIRIVTNSLPVFNILQDSETIDLILIGGEYRSITGAFVGSLASQN
                     ISSLKFAKAFISCNGIYKNDIATYSETEGEIQKLAFNNSIEKYLLVDNQKFNAYDFFV
                     FYHLDNIDAVVTDSQITEEVIERYSQFTQLLVAHENKE"
     gene            11628..12101
                     /locus_tag="VHD02_000176"
     CDS             11628..12101
                     /locus_tag="VHD02_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ5496816.1"
                     /translation="MFPNILFTEARTQPELFDLVASHLEKVGYVTQEYHQALVEREVV
                     FPTGLKVDLKDGSDILYAAIPHTETKYCLVDQVVYVRNSQALTFKHMINPEEDCLVTD
                     FFFIINSQNEGQTTILSNLITFFITKGNLSYLASLKDDKQAISNYLIEKGVFQHD"
     gene            12094..12399
                     /locus_tag="VHD02_000177"
     CDS             12094..12399
                     /locus_tag="VHD02_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ5496817.1"
                     /translation="MIKILAACGAGVNSSHQIKDAIESQMTDRGYDVHCDAVMVKDIT
                     EELVSRYDIFTPIAKTDLGFDLPIPIVEAGPILYRIPIMSEPVFAELERVIKENHLQ"
     gene            12425..13876
                     /locus_tag="VHD02_000178"
     CDS             12425..13876
                     /locus_tag="VHD02_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEQ5496818.1"
                     /translation="MMQPFLDIINKILGFPIQLGSGVVMLIVMTGLAMIFGVKFTKAL
                     EGGIKLAIALTGIGAIIGILTGAFSESLQAFVKNTGISLNIIDVGWAPLATITWGSPY
                     TLYFLLVMLVVNIVMIVMKKTDTLDVDIFDIWHLSITGLLIMWYAARNHLPVFVSLLI
                     ATVAVILVGVLKIINSDLMKPTFDDLLGTGSQSPMTSTHMNYMMNPIIMVLDKIFDKV
                     FPGLDKYDFDAAKLNKKIGFWGSKFFIGMALGFVIGIMGDPHFTVESIKNWFGLGFTA
                     GACLELFSLIGSWFIAAVEPLSQGITNFANARMHGRRFNIGLDWPFIAGRAEIWACAN
                     ILAPIMLIEAVLLSKVGNGILPLAGIIAMGMTPALLVVTRGRLIRMIIFGSLLLPLFL
                     LSGTMIAPFATELAKKVGAFPAGASAGSLITHSTLEGPMEKIFGYVIGQATTGQIASI
                     ITLIIFVAIYLGLFAWYANQMKARNAEYAKTMT"
     gene            14134..14559
                     /gene="lacA"
                     /locus_tag="VHD02_000179"
     CDS             14134..14559
                     /gene="lacA"
                     /locus_tag="VHD02_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEQ5496819.1"
                     /translation="MAIILGADAHGNALKELIKSFLQEESYDIIDVTDINSDFIDNTL
                     AVAKAVNEADGRLGIMVDAYGAGPFMVATKLKGMVAAEVSDERSAYMTRGHNNARMIT
                     MGAEIVGPELAKNIAKGFVTGHYDGGRHQIRVDMLNKMA"
     gene            14574..14990
                     /locus_tag="VHD02_000180"
     CDS             14574..14990
                     /locus_tag="VHD02_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEQ5496820.1"
                     /translation="MKIAIGCDHIVTNEKMAVSDFLKSKGYDVIDCGTGVGINNAVNK
                     VPGIRSALVRDMTTALYAKEELNANVIGFGGKITGELLMCDIIDAFIKAEYKETEENK
                     KLIAKIAHLETHHASQEDPDFFTEFLERWDRGEYHD"
     gene            15000..15442
                     /locus_tag="VHD02_000181"
                     /pseudo
     CDS             15000..15442
                     /locus_tag="VHD02_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061423.1"
                     /note="catalyzes the formation of tagatose
                     1,6-bisphosphate from tagatose 6-phosphate and ATP;
                     frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
     gene            15489..16466
                     /gene="lacD"
                     /locus_tag="VHD02_000182"
     CDS             15489..16466
                     /gene="lacD"
                     /locus_tag="VHD02_000182"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEQ5496821.1"
                     /translation="MTITANKRHYLEKVSHQGIISALAFDQRGALKQMMAAHQEGEAT
                     VTQIETLKVLVSEELTPYASSILLDPEYGLLATKVRANQTGLLLAYEKTGYDATTTSR
                     LPDCLVEWSVKRLKAAGADAIKFLLYYDVDGDEQINLQKQAYIERIGSECTAEDIPFF
                     LELLSYDERISDNNSAAYAKLKPHKVNGAMSVFSDKRFGVDVLKVEVPVNMAYVEGFT
                     EGEVHYSQAEAIKAFQDQEAASHLPYIYLSAGVSAKLFQETLYFAAAAGAQFSGVLCG
                     RATWAGSVPVYITKGEDEARKWLCTEGFQNIDELNRVLEETASPWTEKI"
     gene            16663..17280
                     /locus_tag="VHD02_000183"
     CDS             16663..17280
                     /locus_tag="VHD02_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEQ5496822.1"
                     /translation="MTIDMWEILNIIGTIAFALSGAIVAMEEEFDILGIFILGFVTAF
                     GGGAIRNTLIGLPIEALWGQKPEFTCAFFAMVLIMLFPKLIARGWVRAAVLTDAIGLA
                     AFSVQGALHAVRLNQPLSAVIVAAVLTGAGGGVVRDILAGRKPSVLRSEIYAGWSILA
                     AIVLHFKLADSTIECYALVVLLTTLRMIGNRKKWNLPKIKWKEER"
     gene            17282..18106
                     /locus_tag="VHD02_000184"
     CDS             17282..18106
                     /locus_tag="VHD02_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ5496823.1"
                     /translation="MIQLIAIDLDGTLLNQDKQIPKENITAIQEAAQSGLKIVLCTGR
                     PQSGTRPYFDQLGLTQEEFLIINNGCSTYSSPDWQLRHSKMLKVSDIELLEELSQSFP
                     DIYLTLTEENDYLVLEEEVPDLVQEDGDLVFTIVKPVSLAELSDTPRLIFQAMYLGEK
                     AALDAFERAVRNQLSQSFHVVRSQDNILEILPQGVSKASALKELVEDLGLTADQVMAI
                     GDAPNDIEMLTYAGLGVAMENASAAIKPLADKVTLTNDMAGVAQAIRQFALAAQKD"
     gene            complement(18142..18699)
                     /locus_tag="VHD02_000185"
     CDS             complement(18142..18699)
                     /locus_tag="VHD02_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ5496824.1"
                     /translation="MTDKALSKRSLQNLTQFNKETRRIARESMEIALLNLLEIKPLGD
                     ITISELVTKAGVSRNAFYRNYTSKEAIIEQLLVGVIRRIFRGLKQFDIKTQAYQAWLY
                     LFTEAKKEAQLLKMIFKHHLHHLLTRLVTKRLKAYQKLKDKKQSHYKRLFWSNAIVSV
                     LTNWISDDMIVPAEEMAAMGLPLLT"
     gene            18992..19834
                     /locus_tag="VHD02_000186"
     CDS             18992..19834
                     /locus_tag="VHD02_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ5496825.1"
                     /translation="MTWKIVTDSGCDLRSLTRQSKELRFERVPLTLQIGTEIFRDDDG
                     LDIDNMMTTMYQSSKATTSSCPSPEAFLQAYRGADNVIVMTITGTLSGSHNSARLAKN
                     ELLEENPNVNIHLIDSLSAGGEMDLLVLELERLINLGLSFEEVVKQITAYQQKTRLIF
                     VLAKVDNLVKNGRLSKLVGKVIGLLNIRMVGKASNKGTLELLQKARGQKKAVSALIEE
                     IQKEGYVGGKVYIAHAQNPKICEQISEKIKSLYPDAVIQTGRTSGLCSFYAEDGGLLM
                     GYEI"
     gene            complement(19963..20685)
                     /locus_tag="VHD02_000187"
     CDS             complement(19963..20685)
                     /locus_tag="VHD02_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496826.1"
                     /translation="MATMKQYHKGDSMTKSQKEALYWMLSVLTITLIGGSCLIFGSYP
                     QTQDKVAKHSKSAASLLKKAVKAVNDADRLATAAAIQEAQKAVDKLAESSKKKTLQEQ
                     LNVAKAKQEQEDAATQAVKAAEETLNQNLKDIAQKAVNDLSNKGKKAALQSRLDAILP
                     AKPIIDEFPRQSGEITDNSYWTPFPGDVSDTYDNSQSPTLDPSSESSASDVTPQPSHP
                     DPIPPQTSSEPSDSGDKQSSKE"
     gene            20887..22518
                     /locus_tag="VHD02_000188"
     CDS             20887..22518
                     /locus_tag="VHD02_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEQ5496827.1"
                     /translation="MSVNWQDIAFHFFGGLGLFLFSIKYMGDGLQQAAGDKLRYYIDK
                     YTSNPFFGILVGIAMSALIQSSSGVTVITVGLVSAGLLNLRQAIGIVMGANIGTTITS
                     FLIGFKLGDYALPMIFIGAACLFFTSNKKLNNFGRIIFGVGGIFFSLNLMGDAMDPLK
                     SVSAFQNYLATLGDKPFQGVFIGTALTMLIQSSAAIIGILQGLFSGGLLTLQGAIPIL
                     LGSNIGTCITAVLAAIGSNIAAKRVAAAHVLFNLIGTIIFMIILVPFTSLMLWLQSKL
                     SLTPEMTIAFSHGSFNITNTILLIPFISLLAMIVTRLIPGEDEVVKYEALYLDRLLIT
                     QAPSIALGNAHKELVHLASYAIQAFEASYSYIMTADDKFGEKVKRYERAVDTIDEELT
                     TYLVDISNEALSPSENEVLAGILDSSRDLERIGDHSESLGILIEGIISKQIGFSISAR
                     QELTEMYQLTHCLTLDAIRAIVDSDTDLAQTIVTRHKEIEEKERRLRKTHIKRLNCGE
                     CTAQAGINFIDIISHYTRITDHALNLAEKVLSHQL"
     gene            22636..23784
                     /gene="nagA"
                     /locus_tag="VHD02_000189"
     CDS             22636..23784
                     /gene="nagA"
                     /locus_tag="VHD02_000189"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEQ5496828.1"
                     /translation="MTCYLKADCFYYPTEVKPAGYLSLHDGVFGEWTEIVPADAQIID
                     YTGYQIAPGLVDTHIHGYAGADVMDNSAQGIHQMSEGLLATGVTSFLPTTLTSTFEQL
                     EKVSGTIASVADQVKGAKIQGIYFEGPYFTEKYKGAQNPSYMKTPRLEEFDAWQKAAK
                     GLIKKIALAPERDGVKEFVSAVTKQGVTVALGHSNGTYQEAKEAVQAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVSPIACDILMQQKGHDHVAMITDCMRA
                     GGSPDGDYLLGEFSVVVANGTARLKESGNLAGSILKLKDGLRNVVAWGIATPAEAIHM
                     ATYVPAVSVGIDDVCGQIKAGHAADFIVLDKDLTLVATYLNGQKAFDA"
     gene            23906..24745
                     /locus_tag="VHD02_000190"
     CDS             23906..24745
                     /locus_tag="VHD02_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEQ5496829.1"
                     /translation="MVTTVKMTSGYEIPVLGFGTYQAADGEEAYQSTLAAIKAGYRHI
                     DTAAIYKNEESVGRAIKDSGVLREDLFITTKLWNDAHSYEGAKDALAASLDRLGLDYV
                     DLYLIHWPNPKALRNTWKEANAQAWQYMEEAVEAGLIKSIGVSNFMVHHLEALQETAK
                     ITPAINQIRLAPGCYQKEVVDYCKANEILLEAWSPLGQGEIFDNETMQQLANKYDKTV
                     AQAALAWSLAEGFIPLPKSVHDERIKENMAIFDVSLTPEDKKTIRYLSGMSAIPNPDT
                     TSF"
     gene            24877..25410
                     /locus_tag="VHD02_000191"
     CDS             24877..25410
                     /locus_tag="VHD02_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEQ5496830.1"
                     /translation="MTIFLVMLAMFTIRLVFLKKSITNEKAILAQGGQEFGAQNTKFL
                     TLLHIMIYVFAVIEALLKQIKFDGISFLGLLLMLLSVAVLYEVTRILGDIWTVKLMLA
                     KDHKYVDHWLFKTIKHPNYFLNIAPELVGIALLCHAKITAMLLFPCYIVVIYLRIREE
                     NKLLAEVIIPNGTRTKP"
     gene            25782..26699
                     /gene="glyQ"
                     /locus_tag="VHD02_000192"
     CDS             25782..26699
                     /gene="glyQ"
                     /locus_tag="VHD02_000192"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEQ5496831.1"
                     /translation="MSKKLTFQEIILTLQQYWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLASLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFLQPEYEHSKYSFEISDQDMLLENFE
                     KFEKEASRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGLPLLDEATRAILLAEDDE"
     gene            27077..29116
                     /locus_tag="VHD02_000193"
     CDS             27077..29116
                     /locus_tag="VHD02_000193"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEQ5496832.1"
                     /translation="MSKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENRLSFEDI
                     QTFSTPRRLAVRVSGLADQQTDLTEDFKGPAKKIALDADGNFSKAAQGFVRGKGLTTD
                     AIEFREVKGEEYVYVTKHEAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPVH
                     TLTVLLNDEALELDFLDIHSGRVSRGHRFLGTETTITSADSYEADLRSQCVIVDAKER
                     QEMIVEQIKTLEVEQGVQVDIDEDLLNEVLNLVEFPTAFMGNFEAKYLDVPEEVLVTS
                     MKNHQRYFVVRDQAGHLMPNFVSVRNGNDQAIENVIKGNEKVLVARLEDGEFFWREDQ
                     KLQIADLVAKLTNVTFHEKIGSLAEHMDRTRVIAASLAKEANLSAEEVTAVDRAAQIY
                     KFDLLTGMVGEFDELQGIMGEKYALLAGEDAAVATAIREHYLPDAAGGALPETKVGVV
                     LALADKLDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILDHFGWRIPMDKLVDSLYD
                     LSFDSLTYTNKADVMNFIRARVDKMMGKAAPKDIREAILASSTFVVPEMLAVAEALVK
                     ASHTENYKPAVESLSRAFNLAEKADASVQVDPSLFENEQENTLFAAIQGLTLAGSAAQ
                     QLEQVFALSPVINDFFDNTMVMAEDQALKNNRVAILSDLVSKAKTIAAFNQLNTK"
     gene            29270..29527
                     /locus_tag="VHD02_000194"
     CDS             29270..29527
                     /locus_tag="VHD02_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEQ5496833.1"
                     /translation="MDPKKIARINELAKKKKTVGLTGPEKVEQAKLREEYIEGYRRSV
                     RHHIEGIKLVDEEGNDVTPEKLRQVQREKGLHGRSLDDPKS"
     gene            29654..30046
                     /locus_tag="VHD02_000195"
     CDS             29654..30046
                     /locus_tag="VHD02_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEQ5496834.1"
                     /translation="MCLSYNRRNQRKKVVLMTIKKFSLLAIASLSLLSLAACDMDDKD
                     DHMDNQPKTSQTSKKVKLSEDKAKSIALKDASVTEADAQMLSVTQDNEDGKAVYEIEF
                     QNKDQEYSYTIDANSGDIVEKSSEPIND"
     gene            30406..31932
                     /gene="glpK"
                     /locus_tag="VHD02_000196"
     CDS             30406..31932
                     /gene="glpK"
                     /locus_tag="VHD02_000196"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEQ5496835.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNQKGEKVSSSQKEFPQIFPHAG
                     WVEHNANQIWNSVQSVIAGAFIESSIKPSQIEAIGITNQRETTVVWDKKTGVPIYNAI
                     VWQSRQTAPIAEQLKQDGHTKMIHEKTGLVIDAYFSATKIRWILDHVPGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAVHVTDYSNAARTMLYNIKDLTWDDEILELLNIPKDMLPEV
                     KSNSEIYGKTAAFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GDEMQLSSNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLKMIETSPESEQFA
                     LASTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRDV
                     IDTMQVDSGIDIQQLRVDGGAAMNNMLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     AVGYWEDMDALKELNATGQLFKASMNESRKEKLYKGWKRAVKATQVFTQEEDADDDAK
                     "
     gene            31948..33786
                     /gene="glpO"
                     /locus_tag="VHD02_000197"
     CDS             31948..33786
                     /gene="glpO"
                     /locus_tag="VHD02_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEQ5496836.1"
                     /translation="MEFSKETRRLALQKMQERDLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAQGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVSNTPAANKVLTKEEVLKREPDLKQEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGALVASHVKAEDFLLDDNGKIIGVKARDLLS
                     DQEIIIKAKLVINTTGPWSDEIRQFSHKGQPIHQMRPTKGVHLVVDRQKLPVSQPVYV
                     DTGLNDGRMVFVLPREEKTYFGTTDTDYTGDLEHPQVTQEDVDYLLGVVNNRFPNANV
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKVSDDSFNHLVDTVKAYINHEDSREAVE
                     KAIKQVETSTSEKELDPSAVSRGSSFDRDENGLFTLAGGKITDYRKMAEGALTGIIQI
                     LKEEFGKSFKLINSKTYPVSGGEINPANVDSEIEAYAQLGTLSGLSMDDARYLANLYG
                     SNAPKVFALTRQLTAAEGLSLAETLSLHYAMDYEMALKPTDYFLRRTNHLLFMRDSLD
                     ALIDPVINEMAKHFEWSDQERVAQEDDLRRVIADNDLSALKGQQEG"
     gene            33788..34489
                     /locus_tag="VHD02_000198"
     CDS             33788..34489
                     /locus_tag="VHD02_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEQ5496837.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKTHASGWIVIATGWG
                     IAVAVAVFISGKVAPAHLNPAVSLAFAMSGTIAWSTAIAYSLAQLLGAMVGSTLVFLQ
                     FRPHYLAAESQADILGTFATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLG
                     TLCVGTLVIGIGLSLGGTTGYAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGP
                     IIGAALAVLLFQVMS"
     gene            34598..35944
                     /locus_tag="VHD02_000199"
     CDS             34598..35944
                     /locus_tag="VHD02_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEQ5496838.1"
                     /translation="MTLTTKTIHVIGASFAGLAFVDKYKDLNPDSQIILIDKESCPNY
                     IPNGINQLFRGDIQDLSDAMWGRACLAAQIESNHRFIQAEVLAIEAPSNTLLLKDSQG
                     RVFEEGYETLVCAMGASPQSHYIETSQTNKVLVTKYYEESQASLKLIEASQEVLVIGA
                     GLIGLDLAYSLSLQGKRVKLIEAAERPDFYQTDAELIAPVMAEMSTHHVTFINNKRVT
                     AIHEIEGKVVAHTEQGDTFQGDLAILAINFRPNTHLLQGQVACALDKTILVNENLQTS
                     QANIYAIGDMVSLHFGILGMDYYTPLINQAMKTGQALALHLAGYPIPPLQTVKVLGSS
                     HFGYYRASVGVTEEEAELYMDTCSYLYQNGDSKNLFWLKLIARKTDGILIGAQLLSKT
                     NALVIANQLGQALALKVTDGDLAFQDFLFLQGHSDLAYHLHEACLKLFEKRLRHED"
     gene            35934..37433
                     /locus_tag="VHD02_000200"
     CDS             35934..37433
                     /locus_tag="VHD02_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ5496839.1"
                     /translation="MKIEDLMDKERKAQYRLLVTLYHAKETLRLKDLMRLSNLSKVTL
                     LKYIDNLNHLCREQGLACQLLLEKDSLSLKENGQFHWEDLVALLLKESVAYQILTYMY
                     CHEHFNITNLSVELMVSEATLNRQLAHLNQLLSEFDLALSQGRQLGSELQWRYFYLEL
                     FRHTLTRQGIDALVNQLDASHLATLIERLIGQSLSAEALEQLLIWLAISQARMSFQKG
                     YNDHFLRDSDFMTSNIFFKRLESMLLHYLRRYALEFDAFEAKSLFVFLHAYPLLPIAS
                     MKYSLGFGGPIADHISEALWLLKKAHVIIHQTKEEIIYGLGIFFSKAYFFKGAILSQP
                     TNSQYLYQLVGEDKRALLRVIINHLVLQMGQETDFSQQLSDDILALLVFSIERHHEPL
                     LVGLALGQNKVEAAIAELAIRRHLGHRRDFQLMPYDHQKVYDCLITYQTVYLPRQDLP
                     YYRLKQYSSPYELTALEAFLKDLFQQKNTREEELSLSPAAKSSFAHKTV"
     gene            37559..38203
                     /gene="fsa"
                     /locus_tag="VHD02_000201"
     CDS             37559..38203
                     /gene="fsa"
                     /locus_tag="VHD02_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEQ5496840.1"
                     /translation="MKFFLDTANVAAIKAINELGVVDGVTTNPTIISREGRDFETVIK
                     EICDIVDGPISAEVTGLTADAMVEEARSIAKWHDNVVVKIPMTTEGLKATNILSKEGI
                     KTNVTLIFTVSQGLMAMKAGATYISPFIGRLEDIGTDAYQLISDLREIIDLYDFQAEI
                     IAASIRTTAHVEAVAKLGAHIATIPDPLFAKMTQHPLTTNGLKTFMEDWASFKK"
     gene            38421..40406
                     /gene="tkt"
                     /locus_tag="VHD02_000202"
     CDS             38421..40406
                     /gene="tkt"
                     /locus_tag="VHD02_000202"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEQ5496841.1"
                     /translation="MTFDAIDQLAVNTVRTLSMDAIQAANSGHPGLPMGAAPMAYVLW
                     NHFMNINPKTSRNWSNRDRFILSAGHGSAMLYSLLHLAGYDLSVEDLKNFRQWASKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDIVDHYTFALNGDG
                     DLMEGVSQEAASMAGHLKLGKLVLLYDSNDISLDGPTSMAFTEDVKGRFEAYGWQHIL
                     VKDGNDLEEIAAAIEAAKAETEKPTIIEVKTIIGFGAEKQGTSAVHGAPLGAEGIAFA
                     KKAYQWTHQDFEVPAEVTERFAQGLQARGEKAEQAWNDLFAVYEAEYPELAAEYQKAF
                     TNEAAQVELEAHELGSSMASRVSSQQAIQQISEQVASFWGGSADLSASNNTMVKAETD
                     FQPGHYEGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNFNVIRPADGNETNAAWKRAIAET
                     DRPTMLVLTRQNLPVLEGTKELAEDGLNKGAYILSEAKGDLEGILIATGSEVKLAMDT
                     QEALEAEGIHVRVVSMPSQNIFDEQSAEYKESILPAAVTKRLAIEAGSSFGWAKYVGL
                     AGKTLTIDTWGASAPGNRIFEEYGFTVANATELYKSL"
     gene            40599..40865
                     /locus_tag="VHD02_000203"
     CDS             40599..40865
                     /locus_tag="VHD02_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEQ5496842.1"
                     /translation="MEKKRQRLYDVIRQAYDYPENRENVALSQLLLAASNRLIKHSNP
                     LLVAYQLNQDVDNYLLDNDILLPKSLCRFKQSLEKYILEEASSV"
     gene            40899..41633
                     /locus_tag="VHD02_000204"
     CDS             40899..41633
                     /locus_tag="VHD02_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5496843.1"
                     /translation="MIEFKHVSKLYGDKEALSDLNVTINDGEIFGLIGHNGAGKTTTI
                     SILTSIIEASYGEVFVDGQLLTENREAIKKQIAYVPDSPDIFLNLTPNEYWQFLAKIY
                     GVSDEDREERLAQLTTLFELKEEVNQTIDSFSHGMRQKVIVIGALVSNPNIWILDEPL
                     TGLDPQASFDLKEMMKAHAASGHTVLFSTHVLSVAEQLCDRIGILKKGKLIFVGTIDE
                     LKEHHPDKDLESIYLELAGRKAQEEG"
     gene            41638..43266
                     /locus_tag="VHD02_000205"
     CDS             41638..43266
                     /locus_tag="VHD02_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ5496844.1"
                     /translation="MNWSTIWELIKINILYSNPQSLANLKKRQENHPKENFKAYKSMM
                     RQQALMIAMFLVIYLFMFIGVDFSHYPGLFSFDVAMFFIMSTLTAFSSLYTIFYESND
                     LKLYIHLPVTSEELYIAKIVSSLGMGAVFLMPLISLLLIAYWQLLGNPLSILVAIVLF
                     LVLLVSSMVLAIYINAWVGKIIVRSRKRKLISTIMMFVSTFGAFVLIFAINISNNKRT
                     MTDGVFTDYPTIPYFKGFYDVIQAPFSTAALLNFWLPLLLILAMVYGIVTKVMPTYYR
                     EAFYISNENKAKQTKKPVNRPHQNQSLTQLLRKHHLLTLQNATLLTQTYLMPLMYVML
                     FIGPSLSRGTGFFKHISPDYFGVALLFGVSLGVMCATPTSFIGVGISLEKDNFTFIKS
                     LPITLKKFLLDKFCLLVGLQLIVPMVIYLVFGLFVLHLHPLLTIAFCLGYALSLIVQG
                     ELMYRRDYRLLDLKWQDMTQLFTRGNGQWLTMGLIFGNLIVTGVVGFGAVIIANIIQQ
                     PLLISILLSCLTLMVLGIAHLWIQKTFWKSLEKL"
     gene            43330..44151
                     /gene="proB"
                     /locus_tag="VHD02_000206"
     CDS             43330..44151
                     /gene="proB"
                     /locus_tag="VHD02_000206"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEQ5496845.1"
                     /translation="MMKRQFEDVTRIVIKIGTSSLVLPTGKINLEKIDQLAFVISSLM
                     NKGKEVILVSSGAMGFGLDILKMEKRPTNLAKQQAVSSVGQVAMMSLYSQIFAHYQTN
                     VSQILLTRDVVVFPESLANVTNAFESLISLGIVPIVNENDAVSVDEMDHATKFGDNDR
                     LSAVVAGITKADLLIMLSDIDGLFDKNPTIYEDAQLRSHVAVITQEIIASAGGAGSKF
                     GTGGMLSKIQSAQMVFENKGQMVLMNGANPRDILRVLEGQPLGTWFKQIEEVTHD"
     gene            44144..45394
                     /locus_tag="VHD02_000207"
     CDS             44144..45394
                     /locus_tag="VHD02_000207"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEQ5496846.1"
                     /translation="MTDMRRLGQRAKQASLLIAPLSTQIKNRFLSTLAKALVDDTQTL
                     LAANQKDLANAKEHGISDIMMDRLRLTSERIKAIAQGVQQVADLADPIGQVIKGYTNL
                     DGLKILQKRVPLGVIAMIFESRPNVSVDAFSLAFKTNNAIILRGGKDALHSNKALVKL
                     IRQSLEKSGITPDAVQLVEDPSHAVAEELMQATDYVDVLIPRGGAKLIQTVKEKAKVP
                     VIETGVGNVHIYVDAQADLDMATNIVINAKTKRPSVCNAAEGLVIHEAVAARFIPMLE
                     KAINQVQPVEWRADDKALPLFEQAVPAKAEDFETEFLDYIMSVKVVSSLEEAISWINQ
                     HTSHHSEAIITRDIKAAETFQDLVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKMHA
                     RGPMGLEALTSTKFYINGDGHIRE"
     gene            45414..45635
                     /locus_tag="VHD02_000208"
     CDS             45414..45635
                     /locus_tag="VHD02_000208"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496847.1"
                     /translation="MTNEREKEEAKVALEELLHQIQVIHLELKIVRNWGYFDLFGDGM
                     LVSWIKRRKMSKVNQLLIDLQVTLQNCRR"
     gene            45678..45842
                     /locus_tag="VHD02_000209"
     CDS             45678..45842
                     /locus_tag="VHD02_000209"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496848.1"
                     /translation="MSWDKIFDIWLDNLLTDCCVIKEAKNFLQVVEILEAAAKSVLAK
                     LADSEQKEQD"
     gene            45866..46879
                     /gene="rsmH"
                     /locus_tag="VHD02_000210"
     CDS             45866..46879
                     /gene="rsmH"
                     /locus_tag="VHD02_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEQ5496849.1"
                     /translation="MKSFSLTFSFLNLLKYGTIKVMTKEFHHVTVLLHETVDMLDIKP
                     DGIYVDATLGGSGHSAYLLSKLGEEGHLYCFDQDQKAIDNAQVTLKSYIDKGQVTFIK
                     DNFRHLKARLTALGVDEIDGILYDLGVSSPQLDERERGFSYKQDAPLDMRMDRQSLLT
                     AYEVVNTYPFNDLVKIFFKYGEDKFSKQIARKIEQARAIKPIETTTELAELIKAAKPA
                     KELKKKGHPAKQIFQAIRIEVNDELGAADESIQDAMELLALDGRISVITFHSLEDRLT
                     KQLFKEASTVDVPKGLPLIPEDMKPKFELVSRKPILPSHSELTANKRAHSAKLRVAKK
                     IRK"
     gene            46884..47207
                     /gene="ftsL"
                     /locus_tag="VHD02_000211"
     CDS             46884..47207
                     /gene="ftsL"
                     /locus_tag="VHD02_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEQ5496850.1"
                     /translation="MTNEKRTQAVTNALQKRIKTFSRIEKAFYTAIIVTAITMAVSII
                     YLQSRKLQLQQEITSLNSHISDQKLELNNAKQEVNELSRRDRIIDIAGKAGLSNRNNN
                     IKKVE"
     gene            47211..49466
                     /locus_tag="VHD02_000212"
     CDS             47211..49466
                     /locus_tag="VHD02_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ5496851.1"
                     /translation="MKKWQKYVLDYVVRDRRTPVENRVRVGQNMMLLTIFIFFIFIIN
                     FMIIIGTDQKFGVSLSEGAKKVYQETVTIQAKRGTIYDRNGTAIAVDSTTYSIYAILD
                     KSFVSASDEKLYVQPSQYETVADILKKHLGMKKTDVIKQLKRKGLFQVSFGPSGSGIS
                     YSTMSTIQKAMEDAKIKGIAFTTSPGRMYPNGTFASEFIGLASLTEDKKTGVKSLVGK
                     TGLEASFDKILSGQDGVITYQKDRNGTTLLGTGKTVKKAIDGKDIYTTLSEPIQTFLE
                     TQMDVFQAKSNGQLASATLVNAKTGEILATTQRPTYNADTLKGLENTNYKWYSALHQG
                     NFEPGSTMKVMTLAAAIDDKVFNPNETFSNANGLTIADATIQDWSINEGISTGQYMNY
                     AQGFAFSSNVGMTKLEQKMGNAKWMNYLTKFRFGFPTRFGLKDEDAGIFPSDNIVTQA
                     MSAFGQGISVTQIQMLRAFTAISNNGEMLEPQFISQIYDPNTASFRTANKEIVGKPVS
                     KKAASETRQYMIGVGTDPEFGTLYSKTFGPIIKVGDLPVAVKSGTAQIGSEDGSGYQD
                     GGLTNYVYSVVAMVPADKPDFLMYVTMTKPQHFGPLFWQDVVNPVLEEAYLMQDTLTK
                     PVVSDANRQTTYKLPNFVGKNPGETSSELRRNLVQPVVLGTGSKIKKVSHQPGQTLTE
                     NQQVLILSDRFVEVPDMYGWTKSNVKTFAKWTGIDISFKGTDSGRVMKQSVDVGKSLK
                     KIKKMTITLGD"
     gene            49468..50478
                     /locus_tag="VHD02_000213"
     CDS             49468..50478
                     /locus_tag="VHD02_000213"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEQ5496852.1"
                     /translation="MFLTLIAAIISFMVSAFTMPYFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLLVATAVSLLVSLFSIKNTQSLALISGILSIVVIYGIIGFLDDFL
                     KIFKQINEGLTAKQKLALQLVGGLMFYFLHVSPSGISSINVFGYQLPLGIFYLFFVLF
                     WVVGFSNAVNLTDGIDGLASISVVISLVTYGVIAYVQSQFDVLLLIGAMIGALLGFFC
                     FNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLIIGIVYVLETSSVMLQVSYFKY
                     TKKKYGEGRRIFRMTPFHHHLELGGLSGKGKKWSEWQVDAFLWGVGSLASLLVLAILY
                     VF"
     gene            50636..51979
                     /locus_tag="VHD02_000214"
     CDS             50636..51979
                     /locus_tag="VHD02_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ5496853.1"
                     /translation="MSFKDYHFKQYVQQALEEIGFVNPTEVQKRLIPIVNSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDEAKAEVQVVITAPSRELATQIFDACKQIAKHFQEEIRL
                     ANYVGGTDKLRQIEKLKGSQPHIVIGTPGRIYDLVKSGDLAIHKATTFVVDEADMTMD
                     MGFLDTVDKIAASLPKSVQILVFSATIPQKLQPFLKKYLTNPVIEQIKTKTVIADTID
                     NWLVSTKGRDKNGQLLEILKTMQPYMAMLFVNTKERADDLHAFLTANGLKVAKIHGGI
                     PPRERKRIMNQVKKLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGMAGTAITLYQPSDDSDIKELEKMGIAFTPKVLKNGEFQDTYDRDRRQNREKAYQK
                     LDTEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKERRAENRAKGRAERKAKKQHF"
     gene            52174..52977
                     /locus_tag="VHD02_000215"
     CDS             52174..52977
                     /locus_tag="VHD02_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ5496854.1"
                     /translation="MTSVFLTSGWAFYDYLISPIPHGKLFSWHAVFDAIPNIIQRLPI
                     TLGLTLSGATFGLVLALIFALVKINKVKLLYPIQAIFVSFLRGTPILVQLMLTYYGIP
                     LFLKFLNQKYGFDWNVNAIPASIFAITAFAFNEAAYASETIRAAILSVDTGEIEAAKS
                     LGMTSVQVYRRVIIPNATVVAIPTLINGLIGLTKGTSLAFNAGIVEMFAQAQILGGSD
                     YRYFERYISVALVYWSISILMEQVGRLIENKMAIKAPEQARNEKLGELR"
     gene            52977..53720
                     /locus_tag="VHD02_000216"
     CDS             52977..53720
                     /locus_tag="VHD02_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ5496855.1"
                     /translation="MITIRNLSKTFSGQKVLDSLALDIEKGQVIALVGASGAGKSTFL
                     RSLNYLEKPDSGSISIGDFTVDFETITTEQVLILRRKLAMVFQQFNLFERRTALENVK
                     EGLKVVKKLSDQEATKLAQAELAKVGLADRKHHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSITDAAKSGQTMVLVSHDMNFVYQVADRVLFLDQGKIL
                     EQGTPEEVFRHPQKERTKEFFASYSKTYI"
     gene            53824..54048
                     /locus_tag="VHD02_000217"
     CDS             53824..54048
                     /locus_tag="VHD02_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEQ5496856.1"
                     /translation="MLVEIFSLYVKGLILSSLTMILICLIWTLWRAKTKRDKTLIERQ
                     AFLYEMLMVAILTIPILSFAFMSILVVIKS"
     gene            54112..55029
                     /gene="trxB"
                     /locus_tag="VHD02_000218"
     CDS             54112..55029
                     /gene="trxB"
                     /locus_tag="VHD02_000218"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEQ5496857.1"
                     /translation="MYDTLIIGSGPAGMTAALYAARSNLSVAIIEQGAPGGQMNNTFD
                     IENYPGYDHISGPELAMKMYEPLEKFNVENIYGIVQKIENFGDYKCVLTEDASYEAKT
                     VIIATGAKYRVLGVPGEEYYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAIYLT
                     QFAKKVTVVHRRDQLRAQKILQDRAFANDKVDFIWDSVVKEIQGNDIKVSNVLIENVK
                     TGQVTDHAFGGVFIYVGMNPVTGMVKDLEITDQEGWIITDDHMRTSIPGIFAIGDVRQ
                     KDLRQITTAVGDGAIAGQGVYHYLESFPS"
     gene            55198..56577
                     /locus_tag="VHD02_000219"
     CDS             55198..56577
                     /locus_tag="VHD02_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEQ5496858.1"
                     /translation="MSIKEQTDNNELENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SIALTGPSIIFVYMITGAFMFMMMRAIGEMLYYDPDQHTFINFISKYIGPGWGYFSGL
                     SYWISLIFIGMAEITAVGAYVQFWFPSWPAWLIQLVFLVLLSSINLIAVRVFGETEFW
                     FAMIKILAILALIATAIFMVLTGFETHTGHASLSNIFDHFSMFPNGKLKFFMAFQMVF
                     FAYQAIEFVGITTSETANPRKVLPKAIQEIPTRIVIFYVGALVSIMAIVPWHQLPVDE
                     SPFVMVFKLIGIKWAAALINFVVLTSAASALNSTLYSTGRHLYQIANETPNALTNRLK
                     INTLSRQGVPSRAIIASAVVVGISALINILPGVADAFSLITASSSGVYIAIYALTMIA
                     HWKYRQSKDFMADGYLMPKYKVTTPLTLAFFAFVFISLFLQESTYIGAIGATIWIIVF
                     GIYSNVKFK"
     gene            56748..58202
                     /locus_tag="VHD02_000220"
     CDS             56748..58202
                     /locus_tag="VHD02_000220"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEQ5496859.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFEQGIHNRHAVFEVYFRKEPFN
                     NGYAVFAGLQRMVEYLEQFQFSETDLAYLEELGYPGNFLTYLKELRLELTIRSAKEGD
                     LVFANEPIVQVEGPLGQCQLVETALLNIVNFQTLIATKAARIRSVIEDEPLLEFGTRR
                     AQELDAAIWGTRAAMIGGADATSNVRAGKRFDIPVSGTHAHALVQAYGNDYDAFMAYA
                     KTHKDCVFLVDTYDTLKVGVPTAIRVAKEMGDKINFLGVRLDSGDLAYLSKTVRQQLD
                     DAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     QEDGSMRDTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITFTDINVNELTEIEM
                     FHPTYTYIKKTVRNFDAIPLLVDIFVKGELVYQLPTLSEIKTYAKKEFDKLWDEYKRV
                     LNPQDYPVDLARDVWQNKMTLIDNIRKDTHGKSE"
     gene            58204..59028
                     /gene="nadE"
                     /locus_tag="VHD02_000221"
     CDS             58204..59028
                     /gene="nadE"
                     /locus_tag="VHD02_000221"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEQ5496860.1"
                     /translation="MTLQEEIIRQLGVKASIDPQEEIRKTVDFLKAYLRKHSFLKTYV
                     LGISGGQDSTLAGKLAQMAIAELREETSDKAYQFIAVRLPYGVQADEADAQKALAFIA
                     PDQTLTINIKAAVDGQVEALQAAGVEISDFNKGNIKARQRMISQYAIAGQMAGAVIGT
                     DHAAENITGFFTKFGDGGADILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQK
                     PGLADEVALGVTYQDIDDYLEGKLISKAAQATIEKWWHKGQHKRHLPITIFDDFWK"
     gene            59213..60550
                     /gene="pepC"
                     /locus_tag="VHD02_000222"
     CDS             59213..60550
                     /gene="pepC"
                     /locus_tag="VHD02_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEQ5496861.1"
                     /translation="MSALTETFTEQLFAHYEANAKFSAIENAVTHNGLLKSLETRQSE
                     VDNDFVFSIDLTKDKVSNQKASGRCWMFAALNTFRHKLITEFKLENFELSQAHTFFWD
                     KYEKANWFMEQVIATADQELTSRKVKFLLDVPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESISSSNSRELNQYLNKLLRQDAQILRDLIASGAKADQVEDRKAELLQEIFNFLAMTL
                     GLPPRHFDFAYRDKDDHYHVEKGLTPQAFYDKFVGLKLSDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSREVRYLNLDMKRFKELAIKQMQAGESVWFGSDVGQVSDRQKGILATN
                     TYDFEASMDINLSQDKAGRLDYSESLMTHAMVLTGVDLDETGKPLKWKVENSWGEKVG
                     DKGYFVASDAWMDEYTYQIVVRKEFLTADELAAYEKEPQVLAPWDPMGALAQC"
     gene            complement(61014..63179)
                     /locus_tag="VHD02_000223"
     CDS             complement(61014..63179)
                     /locus_tag="VHD02_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ5496862.1"
                     /translation="MITIKNPKILKWLKYVLSAILSLIILVIIIGGLLFTFYISSAPK
                     LSEAQLKSTNSSLVYDGNNNLIADLGSEKRENVTADSIPINLVNAITSIEDKRFFNHR
                     GVDLYRIFGAAFHNLTSQTTQGGSTLDQQLIKLAYFSTNESDQTLKRKAQEVWLALQM
                     ERKYTKQEILTFYINKVYMGNGNYGMLTAAKSYYGKDLKDLSYAQLALLAGIPQAPSQ
                     YDPYLHPEAAQNRRNVVLQQMYMEKHLTKAEYETAIATPVAEGLQSLQQRSTYPKYMD
                     NYLKQVIEEVKKETNKDIFTAGLKVYTNIIPDAQQTLYNIYHSGDYVYYPDQDFQVAS
                     TIVDVTNGHVIAQLGGRNQDENVSFGTNQAVLTDRDWGSTMKPITAYAPAIESGVYTS
                     TAQSTNDSVYYWPGTTTQLFNWDLRYNGWMTIQAAIMLSRNVPAVRALEAAGLDYARS
                     FLSSLGINYPEMHYSNAISSNNSSSDKKYGASSEKMAAAYAAFANGGIYHKPRYVNKV
                     EFSDGTSKTFDEKGKRAMKETTAYMMTDMLKTVLTYGTGTAAAIPGVAQAGKTGTSNY
                     TDEELAKIGEKYGLYPDYVGTLAPDENFVGFTKRYAMAVWTGYKNRLTPVYGSSLEIA
                     SDVYRSMMTYLTNGYSEDWTMPNGLYRSGGFLYLSGTYASNTDYTNSVYNNLYSNNTT
                     TASSQTTSDDTSSSNDISNSTNTDNNSNHPSTDDKKTTH"
     gene            complement(63166..63774)
                     /gene="recU"
                     /locus_tag="VHD02_000224"
     CDS             complement(63166..63774)
                     /gene="recU"
                     /locus_tag="VHD02_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEQ5496863.1"
                     /translation="MVNYPHNLIRQKVSSVQKQTKQNKVDFANRGMSFEAAINATNDY
                     YLSRQIAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYCGVYKGHYVDFEAK
                     ETRQKTAMPMKNFHLHQIEHMACVLHQKGICFVLLHFSTLKETYYLPAQALISFYQID
                     NGSKSMPIDYIRKNGFKVAFGAFPQVPYLNIIEQNFLGGDYN"
     gene            63855..64370
                     /locus_tag="VHD02_000225"
     CDS             63855..64370
                     /locus_tag="VHD02_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEQ5496864.1"
                     /translation="MTAILITGYRSFEIGIFDHKDPRVSIIKQAIRKDLIGYLENGVD
                     WFIFTGNLGFEQWALEVANELKEEYPLQIATIFLFETHGDRWNEKNQKVLSQFRAVDF
                     VKYYFPNYEQPTQFSQYYQFLLEKTEGAYVFYDTENETNLKYFLKKAKDMPHYQLLLL
                     TFDRLNDMSQS"
BASE COUNT        19606 a        11525 c        14012 g        19344 t
ORIGIN      
        1 tttgattatg tgttgccaca tgagaaagta aagaatggag aagttgtgac agatgaaaaa
       61 gtggaagaag tgttgatgga attggagtaa cattacccgt caatcacccg tttattttta
      121 ttttttcatc tttttctatc ctttctatat tttaaaaata ccaacgttta agtgtcttgc
      181 aaaggatgaa aacggtgaga tagctaccag caatcccctc ctctccttgt gagtaaaaca
      241 aagtgcatat ccttgcattc ctcttaaatt atggtataat aggcttaaat aatacagaaa
      301 ggtggtggga tatgtctcgc cacttttgta ttaagtggac tcccaaggac caattatctt
      361 agagaagagc ataaaaatag aggaggtaat caaaatagca aatacatcta ttatcgatat
      421 cgtaaccaag acagtaacac ctgagattaa ggcaccttat gaattagtag acgttgaata
      481 tgacaaaatg ggtagtgact acatccttag tattttagtc gataaggaag gtggaatcac
      541 agtggaagat acatctgact tgacgaatat tattagtccg ctgttagata cgattgatcc
      601 agatcctttt ccaaaccaat atatgctaga agtctctagt ccaggtttgg agcgtccgct
      661 taagacagct gacagtctaa aagcagcagt tggctcttat atcaatgtga gtctttatca
      721 agctattgat aaggtgaaag tttttcaagg agacttatta gcctttgatg gtgaaacgtt
      781 gaccattgat tatcttgata aaacacgtca taagatcgtg aatataccat atcaagcagt
      841 tgctaaggtg agaatggctg tcaaattata acttttattt caaagcacta aactttttta
      901 gaaacactta agcaaatttg aaacaacacc tatagagttt atacaagcta aacaaaagga
      961 attaagggag cctttagggg gctattgatc gtattaattt aagaaaggat ttttgccaag
     1021 ctttacaagc aaggtagata acattatgag caaagaaatg ctagaagcct tccgtatttt
     1081 ggaagaagaa aaacacattg ataaagcaga tattattgac gcagtgacag aatcattaaa
     1141 gtctgcctat aaacgtcgct atggtcaatc agaatcatgt gttattgaat ttaatgaaaa
     1201 aacggctgat tttcaagtat tcacggttcg tgaggttgtt gaagaagttt ttgacagccg
     1261 gcttgagatt agtttgaaag atgctcttgc tatcagttct gcctatgaat tgggtgataa
     1321 gattcgcttt gaagaatctg tcaatgaatt tggacgtgtc gcagctcaat cagctaaaca
     1381 gactattatg gaaaagatgc gccgccaaat gcgtgaagtg atgttcaatg aatacaaaga
     1441 gcatgaaggt gaaattatga caggaacagt tgaacggttt gatcaacgtt ttatttatgt
     1501 caatcttggt tccttagaag cacaattgtc tcaccaagac cagattccag gtgaaacttt
     1561 caaatcacat gaccgtattg acgtttatgt ctacaaagtt gaaaataatc ctaaaggagt
     1621 taacgtcttt gttagtcgta gccatccaga attcatcaaa cgtattatgg agcaagaaat
     1681 tcctgaagtc tttgatggta ctgttgaaat catgagtgtt tctcgtgaag caggtgaccg
     1741 caccaaggtt gctgtgcgca gtcataatcc taacgttgat gccatcggaa caattgttgg
     1801 acgtggtgga agtaatatta aaaaggtcat cagcaagttc catccaaaac gtgtagatgc
     1861 taagacgggg cttgaaattc cagttgaaga aaatattgac gtgattcaat gggtagatga
     1921 tccagctgag ttcatctaca atgccattgc tccggctgaa gtagacatgg tactttttga
     1981 cgatgaagat cttaaacgtg ctacggtagt agttcctgat agcaaattat cattagctat
     2041 cgggcgtcgc ggccaaaatg ttcgtttagc ggcacatttg actggctacc gtattgatat
     2101 caaatcagct agcgaatatg atcgtcttga ggcggaaaaa gaagcagcaa cagctgtgga
     2161 agagccagtt gttgacgatg ttatgactgc cgaggaagta taagaaaaga ggtgtttgat
     2221 gtctaaagtc aaaaaaatac ctttacgaaa atcacttgtt tctggtgaaa ttattgctaa
     2281 acgagatctt cttaggattg taaaaactaa ggatggtcaa gttttcattg atccaactgg
     2341 taaacaaaat ggtcgtggtg cttatatcaa attagacaat caagaggcct taatggccaa
     2401 gaaaaaacaa gttttcaaca ggagtttctc aatggacatt ccagaaagtt tttacgatga
     2461 cttgattgct tatgttgatc acaaaatcaa aagaagagag ttaggtcttg actaatttag
     2521 aaagattatc tagtcttatt ggtctagctc aacgggctgg aaaagtgatt tcaggtgaag
     2581 aactagtagt aaaagctatt caacaccagc aggtaatatt agtattttta gccaatgatg
     2641 ctggtcctaa tgtgacaaaa aaagtgacag ataaaagtaa ttattataat gtagaagtct
     2701 ccacagtgtt gaatgcactg gaattaagtg ctgcactagg taaaccaagg aaggttgctg
     2761 ccattgcaga tgctggattt tcaaagaaaa tgaggactct tatggaatag atgaatagga
     2821 ggacacaaat tgtcaaagaa aagattacat gaaattgcca aggaaattgg taaaagtagt
     2881 aaagaggtgg ttgaacacgc taaatactta ggattagatg tgaaaagcca tgcatcaagt
     2941 gttgaagaag cggatgcgaa aaagattatt tcaagttttt caaaggcttc taaacctgat
     3001 gtgacagctt ctcaaacagt caaacccaaa gaagttgctc aaccctctgt aacagtggtc
     3061 aaagaaacag gttctgagca tgtagagaaa acacaggtct ctaagccaaa gagtcgtaat
     3121 tttaaagctg aaagagaggc tcgtgcaaag gaacaggctg ctcgaaaaca agcaaatggc
     3181 tcttctcatc gttctcaaga acgtcgagga ggttatcgcc agcccaataa tcatcaaact
     3241 aacgaacaag gagataagag gattactcat cgttctcaag gtgatacaaa cgataagcgt
     3301 attgagcgaa aagcatcgaa tgtatcacct agacatgata atcatcagct agttggtgat
     3361 cgtaaccgtt cctttgctaa ggagaatcac aagaatggtc gattcacaaa ccaaaaaaag
     3421 caaggacgac aagagcccca atcgaaaagt cctaaaattg atttcaaagc tcgtgctgct
     3481 gccttaaaag ctgaacaaaa tgctgaatac tcacgccaaa gtgaaacacg ttttcgtgca
     3541 cagcaagaag ctaagcgttt agctgagttg gcaagacaag aagctaaaga agcggctcta
     3601 aaagcacaag ctgaggagat gagccatcgt gaagcagcac tgaaatctat tgaagaagca
     3661 gaaactaaat taaagtcatc aaatattagt gctaaatcta ctgctgataa tcgccgtaaa
     3721 aaacaagcac gtcctgaaaa gaatcgtgag ttaactcatc attcacaaga aggacaaaag
     3781 aaaaataaga agagttggaa tagtcaaaac caagtgagaa atcaaaaaaa tagtaactgg
     3841 aataaaaata aaaagaccaa aaaaggcaaa aatgttaaga ataccaacac cgctccaaaa
     3901 ccagtaaccg agcgtaaatt ccatgaattg ccaaaagaat ttgaatatac tgaaggcatg
     3961 acagttgccg aaatcgcaaa acgtatcaaa cgcgaacccg ctgaaattgt taaaaagctg
     4021 tttatgatgg gtgttatggc aactcaaaac cagtctttag atggggatac tatcgaatta
     4081 ttgatggtag attatggtat tgaggctaag gctaaggtag aagtagatga tgctgatatc
     4141 gagcgcttct ttgaggatga aaattacctc aatcctgaaa atattgtaga acgcgcacct
     4201 gttgtaacta tcatgggaca cgtagaccac ggtaagacaa ctttgcttga tactttgcgt
     4261 aactcacgtg tggcaacagg agaagctgga ggaattactc agcatattgg tgcttaccag
     4321 attgaagaag ctggcaagaa aattaccttc ttggatactc ccggacacgc ggcctttaca
     4381 agcatgcgcg cacgtggagc ttccgttacc gatatcacca ttttgattgt tgcagctgat
     4441 gatggtgtta tgccacaaac gattgaagcg atcaatcact caaaagctgc tggtgttcca
     4501 attattgttg ctatcaacaa aattgataaa ccaggtgcta atccagaacg tgttatcgca
     4561 gaattggcag aatatggtat catctcaact gcttggggcg gagagtgcga atttgtagag
     4621 atatctgcta aatttaataa aaatattgat gaactattag aaacagtcct tttggttgct
     4681 gaagtggaag aattgaaagc tgatccgacg gttcgtgcta ttggtactgt aattgaagca
     4741 cgcttagata aaggtaaagg tgctatcgct acgcttcttg tccaacaagg aactcttcat
     4801 gttcaagacc caatcgttgt tggtaatacc tttggtcgtg tccgtgccat ggtaaatgac
     4861 cttgggcgtc gtgttaaatc tgcggagcca tcaacaccag tttccattac tggtttgaac
     4921 gaaacaccaa tggctggtga tcactttgct gtttacgctg atgaaaaagc tgcgcgtgca
     4981 gctggtgagg agcgatcaaa acgtgccctc ctcaaacaac gtcaaaatac acagcgtgtt
     5041 agccttgata atctctttga tacccttaaa gcaggagaaa tcaagactgt taatgtcatc
     5101 atcaaagcag acgttcaagg ttctgttgaa gcccttgcag catctcttgt aaagattgaa
     5161 gtggaaggtg tccgtgtcaa tgtcgttcac tctgctgtag gtgctatcaa tgagtctgat
     5221 gtgacccttg ctgaagcatc aaatgctgtt attatcggtt tcaacgttcg ccctacccca
     5281 caagctcgtc aacaagcaga tacggatgat gtggaaattc gtcttcatag tattatctat
     5341 aaagtcattg aagaagtaga agaagccatg aaagggaaac ttgaccctgt ttaccaagaa
     5401 aaaattcttg gtgaagccat tattcgtgaa accttcaaag tttctaaagt gggaacaatt
     5461 ggtggcttta tggtaatcaa cggtaaggtg acacgtgatt cgagtgttcg tgttatccgt
     5521 gatagtgtcg ttatttttga tggtaaactg gccagcttga aacattacaa agatgacgtt
     5581 aaagaagtcg gtaatgctca agaaggtggg ttgatgattg aaaactttaa cgatcttaaa
     5641 gttgacgata ctattgaagc ctatattatg gaagaaattg tgagaaaata ggggctaaga
     5701 aggtctgaga tgtaacgttt ttcctctgtc ggaaataatg actttttcta ccagatgagc
     5761 tacttagggc attccttcca gtctcgtcat ggaattctca gattcccttt aattgtcgtt
     5821 atcaaaacag gtagcaatgt cataagctga gctttcagcc tagcttttgt tttaaaaatg
     5881 tggaaaggaa atggctatgg ctaatcatcg tattgaccgc gttgggatgg aaatcaagcg
     5941 cgaagtaaat gatattcttc agaaaaaagt gcgtgatcca cgcgttcagg gagtaacgat
     6001 tacagaagtg caaatgcagg gtgacttgtc acttgctaaa gtttattaca ctattatgag
     6061 tgacttggca tctgataatc aaaaagcaca aacagggctt gaaaaagcga ctggaacgat
     6121 taaacgtgaa cttggcaaac aattgaccat gtacaaaatt ccagatttgg tgtttgaaaa
     6181 agacaactcc atcgcctatg gtaataaaat tgatcaactc ttacgagaac ttgacaacaa
     6241 atcctaagta ttgcggtgcg gatgttatga cagaaagagc cttatcatta tgatagggct
     6301 ctttttaatt gttattttgt ggtgcaaact acaaaaaaaa caccagatta actggtggca
     6361 ggtaggaatt attcccctca ttgaggagtt ttgttgatgg catcaatgat ttggtattga
     6421 gctgtttttg cctcttcgat aggataaatt gggtaaacgt ggttcatgcc ttcttgggta
     6481 atgaaggtaa tattgtggtt agcggctttc agttttgcgg cataatcgcg aatatcagga
     6541 taaaagatct ctcgtgtccc agtaaataaa gtaattggtg gtagataagt gatcggacca
     6601 tttttaggac tgacataaat atggttggta ttatcagcag agtaggccca caattctccc
     6661 actcgtttta atccccaaga agatagaata ggatcagcat cttcgtagtt tggaatctct
     6721 gggtgtgaca tagtaacatc taaccaaggt gataaaagaa cgagctgctt tggttgaggg
     6781 actgattcat tgtgtagggc atgagctaga cctaaggcta aacctccacc tgctgaatct
     6841 cccataagga aaatattctg atttttatgg taatagtgtt ggtaaagatt gaccaatttt
     6901 ggcatagtat agttataggt ataacgtggg gctttaggat aaatgggtaa aacaatttta
     6961 gcgtcaagac ttgtggataa tgtctttaac atattaatat ggtatgtcgt tggattgtta
     7021 agataagaac caccggcaag ataaataatt gttttttgat caggttttcc attatcattc
     7081 caagagtaaa cctgcatgcc ataaagattt tcttgaacaa ggggaccatt gactaaatta
     7141 gtatctagtt gataaggttt atcagattgt tcacgacagg catcaaggta tctttggaag
     7201 cttgggtcat cttgtttaga atagtaactc ttgtcacgtt ttaaccagaa atattgctcg
     7261 ataaaccagc tcttccaaga acgagaacta gctgcaactg gagtcggcag gcataaggtc
     7321 agtaatagac ctaaggtgac aagtgttttt cgaataggat gtttcaaggg tatctcctta
     7381 atgtaaatgt tatttttatt gcaataatat tatagtaaaa acgattcatt tgtcaatata
     7441 aggatagaaa aatgagaaaa gttatttatt tacaaatgta gttaaattcg ttataattaa
     7501 tttacaaacg taaatatatt gtgaatgaag gtgacgttat gcaacagata tcagctgccg
     7561 aatgggaagt catgcgtgtg gtgtgggcca gtggtgatat taaaagtagc gatattatta
     7621 ctattttaag gaaaaaatat caatggtcag attccactat taagaccctc attggtaggc
     7681 tagttaaaaa aaatctttta acatcttatc gggaaggtag ggcctatatt tatcaagctc
     7741 ttttggatga gactttggta caaaaagaag cactagcgac tgttttagac ggtatttgtc
     7801 agcggcaaca cacgcgtcta cttcttgaac ggctttatga cttaccgatg accttagagg
     7861 aaattgaggc attccaagag ctactagaag tcaaaaaaga aaatgcagtt cttgaggtgc
     7921 cgtgtaattg cctgcctggg cagtgccact gtcagaaaaa ggagacgtat taatggctaa
     7981 agagatattt ttagtggagg gaatgagttg tgcctcttgt gctctaacaa ttgaaaaaac
     8041 ggtcaatcaa ttgcctgaag tagaacaggc tgttgtgaat ttagcgaccg agaaactcac
     8101 ggttacttat caagatgacg atttagaaac ggccaaagtt attcaagcgg taaaagctgc
     8161 tggttatggt gcacaagtct ttgatgaaca tgcaaaacac atgcaaacag gtcgtcgtga
     8221 cgaagaccta aaggttattt ggtctcaggt cttgtggtct gccctattca ccattcctat
     8281 attataccta gctatgggtc acatggtggg cttatggctt ccttcatttc tgcaaccaga
     8341 tcgctatcct ttcgtttaca gctttagtca gctactcttg acgattccag tcttagtatt
     8401 aaatcgtcat tattaccata atgggttcaa agctttgttc aagggacatc ctaatatgga
     8461 ctccttggta gctttagcaa ccagctttgc ttttgcttat agtctttatg gagtggttga
     8521 aatcagtttg ggccaggcgc actttgtgca ctccctttat tttgaatcgg tggtagtgat
     8581 tttgacactc atcggtctag gaaagtattt tgaaagtcgt tctaagggcc gtacttctca
     8641 agctattcaa aagctcctat ccttgaaggc tactgatgtg agggtatgga gaaacagcca
     8701 atggcaattg ttaccattag aagaagttag ctatgatgac cttgtcctgg ttcaaccagg
     8761 tgaaaaagtt tctattgacg gtattatcgt ggaaggacat agcagtttgg atgaatcctt
     8821 tttaactggg gaaagtttgc ctgttgaaaa gggagaaaaa gataatgtct ttgcgggcag
     8881 cttgaatggg ccaggcgccc tcacgattaa accagataag cttgggaatg ataccttgtt
     8941 agcacaaatt atccaactag ttgagaatgc tcaagaaaat aaagcaccta ttgcagctat
     9001 tgctgataga gtctcaggtg tctttgttcc ggtggttttg gtattagctc tgttaacagg
     9061 ggtattttgg ttggtattca tgcaggaaag tcttcgtttt agtttgacaa cggccatcgc
     9121 tgttctagtc attgcttgtc cttgtgcgct tggtttagca actccgacag cgattatggt
     9181 tggaacaggg cgtgcagctg aaaatggtat tctttttaaa ggtggtgaca tcttagaaca
     9241 agctcatcag gtagatacgg ttgtttttga taaaacgggg acggttacgg aggggaaacc
     9301 aagtcttcaa aagctcctaa ttttttcggg agatgatcac cttatcttgc aagaagctgc
     9361 ttccttggag gcctattctc aacacccctt aggggaggct attgttcgtg ctgctcagga
     9421 agctggttac gatagtctac cagtggaaca gtttgagtct ttaaccggcc taggagtcac
     9481 tggtcagctt gaagggcgtc gtttggcgat tggaaatgct actttaatgg caattcttgg
     9541 tatcgattta tcttgtgttc agtcagtttg tgctaaggct tcaggaaaag gccaaaccct
     9601 tgtatattat gctaaagagg ggcagctacg agctcttttc agtattgctg acgctgtcaa
     9661 agaagatagt caagctaccg tcgaagcttt gcagcaactg gggattcaca cgatcatgct
     9721 aacaggtgac cacgatgcta ccgctaaggc tattgcctct caagtgggga ttacagatgt
     9781 gattagtcaa gtcttacctg atcaaaaagc aggcgtgata gcagacctcc gctctcaagg
     9841 gaaaaaagtt gctatggtag gtgatggtat taatgatgct ccggcattgg ccgtagcaga
     9901 catcgggata gctatggggt cggggacaga tattgcgatt gaatcggcgg atgtgattct
     9961 gatgaggtct gatatgctgg atttggtgaa agtcatgtca ttgagtcgag cgactatgag
    10021 gattgtcaag gaaaatttat tttgggcatt catatacaat gtcctcatga ttccagtggc
    10081 catgggctta cttcatcttt ttggaggccc attattgaac ccgatgttag caggttttgc
    10141 catgagtttt agttcagtat cagttgtttt gaatgcattg cgtttaaaag gaaaaaccat
    10201 ttaaaggaga atacaaaatg gaaaaacatt atcaggttac aggaatgaca tgtgacggtt
    10261 gcgtaagaac ggtcactgaa aaattatcag ctgtccccgg tgtgcaatcg gtccaggtaa
    10321 atttggaaaa aggacaggct aaggtgacag gacgtccttt aacgttccta ctcaagcgtg
    10381 ctttgaagga taccaaattt gaattacatc aatcatctta ggttattttg gtagccttac
    10441 ttcatctcaa aagaggttcc agtttaaact gggcctcttt tttatggtcg ctaataaaga
    10501 ctctgacgtt agcaggtgtg ttccgagcaa aataaaaaca cctctctgga agcaaatcac
    10561 ttttgccaga aggtgccgag ggacgatgcg ctgacaggct gtccattctt gatgaaacgt
    10621 ttactccttg ttttcgtggg ctactaataa ttgggtgaac tgactataac gttcaatgac
    10681 ttcctcagtt atttgagaat ccgtaacaac tgcatcaatg ttatcgagat ggtagaaaac
    10741 gaaaaagtca taggcattga atttttgatt gtctaccaag aggtattttt cgatagagtt
    10801 gttgaaggct agtttttgaa tctctccttc agtttcgcta taagttgcaa tatcgttttt
    10861 ataaatgcca ttacagctaa taaaagcctt ggcaaatttt aaggatgaga tattttgact
    10921 agcaagggac cctacaaaag ctcctgtaat agatcggtat tcaccaccga taagaatcag
    10981 gtcaatggtt tcgctgtcct gaaggatgtt aaagacaggg aggctattgg tcacaatgcg
    11041 aatctgacga ttttttaatt gctcagcgaa acattctaaa gttgttcctg gaccaataaa
    11101 gattgtttca ccatcattga ctagttgtgt cgcatatgaa gctagttccc atttttcctt
    11161 tgtttgaacg gtttgttttt caatatttga ccgctctttt ttattaggca tggtaatact
    11221 ttgtgcacct ccatggatgc ggatcaattt gccggctttt tccagttcat ctaagtctct
    11281 acgaactgtc atatcagaaa cgtttaaagt ctgaataatg tcattcacag taacaatacc
    11341 ttgcttattg actatttcgg taatttttag aagtctttct ctttttaaca tctaaggggc
    11401 tcctttctat agtttattta cttatgttta tcatagcata tagaccttca tatttcaata
    11461 aaacaaaata aacaaaacaa aaacaaacat atagaacaaa aaatattgcg taaaaatgtt
    11521 gaattatgtt gaaggttata ctataatgat tttgtaaacg gttaccaatt tgttgatgat
    11581 gagatgacag ttgttaaaaa ttgatactta tagaaaggcg gtagggagtg tttcctaata
    11641 ttctatttac tgaagcaagg acgcagccag agctttttga tcttgttgct agccacttgg
    11701 aaaaggttgg gtatgtgacg caagagtacc atcaggcttt agtggagcgt gaggtggtat
    11761 ttccgacagg actcaaagtg gacctcaaag atgggtcaga tattttgtat gcggctattc
    11821 cgcatacaga aaccaagtat tgcttggttg atcaagtggt ctatgttaga aatagtcaag
    11881 cccttacctt taagcacatg attaatccag aggaagattg tttggtaacg gatttcttct
    11941 ttattatcaa tagtcaaaac gagggtcaaa cgacgattct atccaatctc attaccttct
    12001 ttattacgaa aggcaatctg agctatttag ccagtctaaa agatgataaa caagctattt
    12061 caaactattt aatagaaaaa ggagtgtttc agcatgatta aaattctagc agcctgcggt
    12121 gcaggtgtta actcaagcca tcaaattaaa gatgctatcg aaagccaaat gactgatcgt
    12181 ggctatgatg tgcattgtga tgcggtgatg gtcaaagaca tcacagaaga attggtgtct
    12241 cgttatgata tttttacgcc gattgcaaaa actgacttgg gatttgacct gccgattccc
    12301 atagtagaag caggaccgat tctctatcgt atcccaatca tgagtgagcc tgtttttgct
    12361 gaattagagc gcgtgattaa agaaaaccat ttgcagtgag ttaataaaaa cataaggaga
    12421 caagatgatg caaccttttt tagacattat taacaagatt ctgggatttc ctattcagtt
    12481 gggatcaggt gtggtcatgc tgattgtcat gacaggttta gccatgattt tcggggttaa
    12541 attcacaaag gcactggaag gcgggattaa gttagctatc gctcttacag gtattggagc
    12601 cattattggg attttgacgg gggctttttc agagtcttta caggcctttg ttaaaaatac
    12661 agggatcagt ttaaacatta ttgatgtcgg ttgggcaccg cttgcaacaa ttacctgggg
    12721 ttccccttac accctttatt tcttgctggt gatgctggtg gttaatattg tcatgattgt
    12781 catgaaaaaa acggacaccc ttgacgtcga tatttttgat atttggcatt tatctatcac
    12841 aggtctattg attatgtggt atgcggctcg caaccattta ccagtctttg tgtctctttt
    12901 gattgctact gtggcagtta ttcttgttgg ggtattgaaa atcattaact ctgatttgat
    12961 gaaaccgacc tttgatgacc ttcttgggac agggtctcaa tcaccaatga cttcaactca
    13021 catgaactac atgatgaatc caatcattat ggtcttagat aagatttttg acaaggtctt
    13081 tccggggcta gataaatatg actttgatgc tgctaaattg aacaaaaaaa ttggcttctg
    13141 gggctctaag ttctttatcg gaatggctct aggttttgtg attgggatta tgggagatcc
    13201 tcatttcact gttgaatcta tcaaaaattg gtttggtcta ggctttaccg cgggtgcctg
    13261 tttggaactt ttctctttga ttggttcttg gtttatcgca gctgttgagc ctttgtcaca
    13321 agggattact aattttgcca atgctagaat gcatggacgt cgtttcaata tcggtttgga
    13381 ctggcctttc attgctggtc gcgctgaaat ctgggcctgt gccaatattc ttgccccaat
    13441 catgttgatt gaagctgttc ttctttcaaa agttggcaat gggattttac cattggcggg
    13501 aattattgct atggggatga caccagctct tcttgtggtc acacgtggcc gtttgattcg
    13561 catgattatt tttggctccc tcttattgcc gttattcctc ttgtcaggaa ccatgattgc
    13621 cccattcgca actgaattgg ctaaaaaagt aggcgctttc ccagcaggtg caagtgctgg
    13681 ttccttgatt acgcattcga ctctcgaagg accaatggaa aaaatctttg gttatgtgat
    13741 tggtcaagcg acaacaggtc agattgcctc aatcattacg ttaatcatct ttgtggccat
    13801 ttacctaggt ctctttgctt ggtatgctaa ccaaatgaag gcacgtaacg ctgagtatgc
    13861 gaaaacaatg acataaaatc aggttaggtg actaactaaa aaactattgg gaatagcaga
    13921 taaccgctta aggtattcct gctttatccc cctagagaga aaagagtatt cctcatgtaa
    13981 ggcaaggcgc tctgtgggta cccgcaagcg cttttcttga aagaaataat ggaagacaac
    14041 taagactgct ggcaataatc ctgacctaag gacaggtaat cctgttacct ttttgtagat
    14101 tggtaagcag tttggattag aaaaggagaa acgatggcta ttattttagg tgcagatgca
    14161 cacggaaatg cattaaaaga attaatcaaa agttttcttc aagaagaaag ctatgacatt
    14221 attgacgtga ctgatattaa cagtgatttt attgataaca cattagcggt ggctaaagcc
    14281 gttaatgaag cagacggtcg tttaggcatt atggtggatg cttacggagc aggtcccttt
    14341 atggtagcca caaaactaaa aggaatggtt gctgctgaag tttcagatga acgctctgct
    14401 tacatgactc gcggtcacaa caatgcccgt atgattacca tgggagcaga aattgtaggt
    14461 ccagaattag cgaagaatat cgccaaaggt tttgtgactg gccactacga tggtggtcgt
    14521 catcaaattc gtgttgacat gctcaataaa atggcttaaa ggaggataaa gagatgaaaa
    14581 ttgcgattgg atgtgaccat attgtcacca acgaaaaaat ggctgtttct gattttttga
    14641 aatctaaagg ctatgacgtt attgactgtg gcactggagt tggcattaac aatgcggtaa
    14701 ataaggtacc aggtattcgt tcagcccttg ttcgagatat gacaacagcg ctttatgcca
    14761 aggaagaatt gaatgccaat gtgattggct ttggtggtaa aatcacgggt gaacttctta
    14821 tgtgtgatat tatagatgct tttatcaagg cagaatacaa agaaactgaa gagaataaga
    14881 agctaattgc taaaatagcc cacttggaaa ctcatcatgc cagccaagaa gatcctgatt
    14941 tctttacaga attcttagaa agatgggatc gtggcgagta tcacgattag gaagaagaga
    15001 tgagtttaac agttatctta aatccttctg tcgctatttc ctatccttta gattgtctgg
    15061 ctatggacac gggacagaac ggcaaaaaca gctggcgata aggagctaaa tgtgaccaga
    15121 atgttagcaa aagctggtca gttatggcaa taggatttag tggtggtaaa ctgggtgact
    15181 ttatcattca tcagttacaa gaacagggca tttctaacca atttttcaaa atcaaagggg
    15241 aaacgagaaa ttgtattgct gttttacatg aaggcatgca gacaaaaatt ttggaagctg
    15301 gtccctacag tgatgtggac gaagccgaag tctctttgag ccacatgagt actattgcta
    15361 agccgtttga tgtcttaatt tctcaggcag tctgcctaag agactagcta ctcactatta
    15421 tcaagatttg atgactatgg caacttttaa acaattatat cagcaacttc aagtaagaga
    15481 ggtatcacat gacaatcaca gcaaataaaa gacattactt agaaaaagtg agccatcaag
    15541 gaatcatatc agctttagct tttgatcaac gaggtgcact gaagcaaatg atggcagcac
    15601 atcaagaagg agaagcgaca gttacacaga tagaaacatt gaaagttctc gtttctgagg
    15661 aattgacgcc ttatgcttca tctattttat tagatccaga atatgggttg ctggctacta
    15721 aggtaagagc taatcagaca ggcttactgt tagcttacga aaaaacaggt tatgatgcta
    15781 caaccactag ccgtttaccc gattgcttag tggaatggtc ggtcaaacgg ttgaaagcag
    15841 caggtgcaga tgctattaaa tttttgctct attatgatgt tgatggagat gaacaaatca
    15901 atttacaaaa acaagcttat attgaacgca ttggttcaga atgtacagct gaagacatcc
    15961 ctttttttct tgaacttttg agttatgatg agagaatatc agataacaac agtgcggcat
    16021 acgcaaagct taagccacat aaggttaacg gtgctatgtc agtattttcg gataagcgtt
    16081 ttggcgttga tgtgctaaaa gtagaggttc ctgttaatat ggcttatgta gaaggtttta
    16141 ctgaagggga agtccattac agtcaagctg aggctattaa agcttttcaa gaccaagaag
    16201 ctgccagcca cctcccttat atctatttga gtgcaggcgt atctgccaaa ctttttcaag
    16261 agacattata ctttgcagca gcagcaggtg ctcaatttag cggtgttttg tgtgggcgtg
    16321 ccacgtgggc aggatccgtt ccagtttata ttactaaagg agaggacgaa gcaagaaagt
    16381 ggctatgcac tgaaggcttc caaaatattg acgagttaaa cagggtactc gaagagacag
    16441 ctagcccttg gacggaaaaa atctaaaaca accttatcct atcaagcagc tctcattgat
    16501 gtgataagat aagtatgggg ataattgtgt gaatcttttg tagcaaggat gaaaatgggt
    16561 tttatccttg cttttttatc cttacatttt atctttttct acttattttt taaaggaaaa
    16621 cgtggtaaac taaaagaaga ctaaaagaca atagggtagc ttatgacaat tgatatgtgg
    16681 gaaattttaa atatcatcgg gaccattgcc tttgctcttt ctggtgctat tgttgccatg
    16741 gaagaagaat ttgatatttt agggattttt attttaggtt tcgtgacagc ctttggaggc
    16801 ggagccattc gaaacaccct aattggttta cctatagagg ccttatgggg gcaaaagcca
    16861 gaattcactt gcgctttctt tgctatggtt ttgattatgc tttttcctaa gctaatagct
    16921 agaggttggg tacgggcagc tgttttgact gatgccattg gtctggcagc ttttagtgtc
    16981 caaggagcct tgcatgctgt ccgtcttaac cagcctttaa gtgctgtcat tgtagcagct
    17041 gtgttgactg gtgcaggcgg tggtgttgtc agagatattc tggcagggcg aaaacctagt
    17101 gttttgcgta gtgaaatcta tgctgggtgg tctattcttg cagctattgt tttgcatttt
    17161 aaactagcag attcaacgat tgaatgttat gccttggttg ttttactcac gactttacgc
    17221 atgattggca accgtaaaaa atggaacctc cctaaaatca aatggaaaga ggaaagatag
    17281 gatgattcaa ctaattgcaa ttgatttgga cggcacctta ttaaaccagg acaaacagat
    17341 tccaaaagag aatatcacag ccatccaaga agcagctcag tcaggcttaa aaattgtctt
    17401 atgtacaggc cggcctcagt cagggacaag accctatttt gaccaattag gtttgacaca
    17461 agaggaattc ctcattatca ataatggctg tagtacttat agtagtcctg actggcaatt
    17521 acgccattca aaaatgttga aggtttcaga tattgaattg ctggaagagc taagccaaag
    17581 ttttccagat atttacctga ctttaactga ggaaaacgat tatttggtgc ttgaagaaga
    17641 agtgccagac cttgtgcagg aagatggaga tttggtattt accattgtca agcctgtctc
    17701 cttagcagag ctgtcggaca ctcctcgcct gatttttcaa gccatgtact taggagaaaa
    17761 agctgcgcta gatgcctttg aacgtgcggt gagaaatcag cttagtcaga gtttccatgt
    17821 tgttcgtagc caagataata ttttagaaat tctccctcag ggagtcagca aggcatcggc
    17881 tctcaaagaa ttagtagaag atttgggttt aactgctgac caagtgatgg ctattgggga
    17941 tgctcccaat gacattgaga tgttaactta tgctggttta ggagttgcta tggaaaatgc
    18001 ttcagcagct atcaagccat tagctgacaa agtcactttg actaatgata tggcgggtgt
    18061 ggcccaggct atccgtcagt ttgcacttgc agctcaaaaa gactaactat taacgtaaaa
    18121 aggttcccaa aaggaacctt tttacgttaa tagaggcaag cccatagcag ccatttcttc
    18181 ggcaggaacg atcatgtcat ctgaaatcca gtttgtcaag acagaaacaa tagcattgct
    18241 ccaaaaaagg cgcttatagt gcgattgttt tttgtctttt aatttttgat aagctttcag
    18301 acgtttagtg actaatcgtg ttagaagatg gtgcaggtga tgtttgaaaa tcattttaag
    18361 aagctgagct tcttttttag cttcagtaaa aagatagagc caggcttgat aagcctgagt
    18421 tttaatatca aattgtttta gtccacgaaa aatacgacgg ataacaccta ctaacagttg
    18481 ttctataatg gcttctttag aggtataatt acgataaaaa gcgttacgag atactcctgc
    18541 tttcgtcacc aattctgaaa tcgtaatatc acctaaaggt tttatttcta gaagatttag
    18601 taaggcgatt tccatagatt cacgtgctat gcgtcgtgtt tctttattaa attgtgttaa
    18661 gttttgaagt gatctttttg agagtgcttt atcagtcatc acaaatacct ttcaagtgtt
    18721 acatctgatg agttatcagt agcaaaaacc gacttaataa tgatataatt ttatgggaaa
    18781 agtcttagtt tgtcaactaa agttagttat caggagcctc attacctcct tgtatagctc
    18841 tattggatga ctttactaac tattgaaaag gctagtaata aaacatagga tttaatgact
    18901 agttattacc attagcctag acatcttagc gaaggcttaa taatctggtc tttgttaccg
    18961 aacacaaatt agagaaaaag aaggataaaa aatgacttgg aaaattgtaa cagattcagg
    19021 atgtgatctt agaagtttga ctagacaatc caaagagttg cgctttgaac gtgtgccttt
    19081 gaccctacag attggcacag aaatttttag agatgatgat ggattagata tcgataatat
    19141 gatgacaact atgtaccagt cgtctaaagc aacgacatcc agctgtccaa gcccagaagc
    19201 ctttttgcag gcctatagag gtgcagataa tgttatcgtg atgacaatta cagggacctt
    19261 atctggcagt cataatagtg cacgactggc taaaaatgaa cttcttgaag aaaatcctaa
    19321 tgtcaatatt cacctaatcg attctttatc agcaggtggc gagatggatt tgctcgtact
    19381 ggagcttgag cggttgatca atcttggatt atcctttgaa gaagttgtca aacagatcac
    19441 agcttatcag caaaaaacac gtttgatttt tgtgcttgcc aaggttgata atttggttaa
    19501 aaatggtcga ctgagtaaat tagtaggtaa agtaattggc ttactcaata ttagaatggt
    19561 tgggaaagct agcaataaag gaacgcttga acttcttcaa aaagcgcgtg gtcaaaaaaa
    19621 agcagtttct gctttgatag aagaaattca aaaagagggt tatgtaggcg gaaaagttta
    19681 cattgctcat gctcaaaatc caaaaatttg tgagcagatc agtgaaaaaa ttaaatcttt
    19741 atatcctgat gctgtgattc aaactggaag gacctcaggt ttgtgtagct tttatgctga
    19801 agacggagga cttttaatgg gctacgaaat ttaatgctat ctaacaaaac aaaaagctag
    19861 actcttttga tttgagctag cttttttaag atatcaggaa gactaatcac taagatagcc
    19921 gtctaaaacg tttattgatt gattttacga aaaatttttg tattactctt ttgatgattg
    19981 cttgtctcct gagtcggaag gttctgaaga ggtctgaggt ggaattggat caggatgact
    20041 aggttgtggc gtaacatctg aagctgaaga ttctgagctt gggtctaaag taggtgactg
    20101 ggaattatca taagtatctg agacgtctcc tgggaaaggt gtccagtaag agttatctgt
    20161 tatttctcca ctttgtcttg gaaattcatc aataataggt ttcgctggta aaatagcgtc
    20221 taaccgagat tgtaaagcgg cttttttacc tttgttactt aagtcgttaa ctgctttttg
    20281 ggcgatatct ttaagatttt gattgagagt ctcctcagca gctttaactg cttgagtagc
    20341 tgcatcttct tgctcttgct ttgctttggc aacgtttagc tgttcttgta gcgttttttt
    20401 cttagaagat tctgctaatt tatcaacagc tttctgggct tcttggatag cagctgcagt
    20461 ggctaaacga tcagcgtcat taactgcttt aactgctttt tttagtaaac tagcagcact
    20521 tttagaatgt ttagctacct tatcttgcgt ttgagggtag gatccaaaaa taaggcagga
    20581 gccacctatt agagtgatag ttagaacact caacatccaa taaagcgcct ctttttgtga
    20641 tttagtcatg gaatctcctt tatgatattg tttcatcgta gccatattat acctctaatt
    20701 aaacgaaatg aaaagatttg tgcaaaaaca aatgatgtaa aatatcggaa aagcctaaca
    20761 ccttatcaaa ccaataatat gcaaatgttg acatcggtat acacagaaac gcttaaaaaa
    20821 accttgtgat aaagaccgta atacattaaa atagaataga ctaaaaaatt gagcgaggac
    20881 aatattgtgt cagtaaattg gcaagacatt gcatttcatt tctttggagg cttaggtctc
    20941 tttcttttca gtatcaagta tatgggagat ggtctgcaac aagcagcagg agataaacta
    21001 aggtattaca ttgataaata caccagtaat cccttttttg gtatcttagt cggaattgcc
    21061 atgtctgcct taattcagtc cagttcaggc gtaactgtga ttacggttgg tttggtttct
    21121 gcgggtttgc taaatttgcg acaagccatt ggaattgtta tgggggctaa tattggaacg
    21181 actatcactt cgtttttaat tggttttaaa ctaggagatt acgccttgcc catgattttt
    21241 attggtgcag cctgtctttt ctttacctct aataaaaagc tcaacaattt tgggcgtatc
    21301 atttttggtg tcggaggaat tttcttctcg ctgaacctaa tgggagatgc catggatcct
    21361 ttgaaatctg tgtcagcctt tcaaaattat ttagcgacat taggtgataa acccttccaa
    21421 ggtgtcttta tcggaacggc tttgaccatg ttgattcagt catcggcagc cattatcggg
    21481 attttacagg gtctgttttc aggcggtctt ttgaccttac agggggctat tcctattttg
    21541 ttaggctcaa atataggaac ctgcattaca gctgtcttag cagctatagg ttcaaatatt
    21601 gctgctaaac gtgtggcagc agctcatgtg ttatttaatt tgatcggtac aattattttt
    21661 atgattatcc tagtcccatt cacatcattg atgttatggc tacaatcaaa gttgagttta
    21721 acacctgaga tgactatcgc tttttctcac ggctctttta atattactaa taccatctta
    21781 ttaattcctt tcattagtct actagctatg attgtgacta gactcattcc aggagaagat
    21841 gaagtggtca aatacgaagc cctttattta gatcgcttac ttattaccca agccccttct
    21901 atcgcactag gaaatgccca caaggaattg gttcatttag cctcttatgc gattcaagct
    21961 tttgaagctt cttacagtta tattatgaca gctgacgata aatttgggga aaaggtcaag
    22021 cgttatgaaa gagcagttga taccatcgat gaggagttaa ccacttattt ggttgatatt
    22081 tctaacgaag ccctcagccc aagtgaaaat gaagtcttgg ctggtattct tgactcttca
    22141 cgtgatttgg aaagaattgg agatcattct gagtctttag gcattctaat tgaaggcatc
    22201 atatctaaac aaattggttt ttcaatatct gctcgtcaag aattgacaga gatgtatcaa
    22261 ttgacgcatt gtttaacact ggatgccatt agagctatcg ttgatagtga tacagacttg
    22321 gcgcaaacta ttgtgactcg ccataaagaa attgaagaaa aagagcgacg attacgtaaa
    22381 acccacatta agcgcttgaa ttgtggagaa tgtacggcac aagcaggtat taattttatt
    22441 gatattattt cccactatac ccgcatcaca gatcatgcct tgaatttagc agagaaagta
    22501 ctttcacacc agctttaaaa aaagagaaaa cgtgaatgat catccacgtt ttcttttttg
    22561 aatccaaaaa gtggtatata ccatttacaa agtaaagaat aagtcgtata ataggggaaa
    22621 aagggaggaa aaaagatgac atgctatctt aaagcagatt gtttttatta cccaacagag
    22681 gtcaaaccgg ctggctacct aagtcttcat gatggtgttt ttggggagtg gacagaaatt
    22741 gttccggcag atgcgcaaat cattgactac accggttatc aaattgcacc aggtcttgtg
    22801 gatacgcata tccatggcta tgcgggagct gatgtcatgg ataattctgc ccaagggatt
    22861 catcaaatga gtgaaggact attagcaaca ggagtaacgt cttttttacc gacgacctta
    22921 acttctacct ttgagcagtt agaaaaagta tcagggacta ttgcttcagt tgctgatcag
    22981 gtaaaaggtg ctaaaataca aggtatttac tttgaaggtc cttattttac cgaaaaatat
    23041 aagggtgctc aaaacccaag ttatatgaaa accccacgct tggaggaatt tgatgcttgg
    23101 caaaaagccg ctaaagggct catcaaaaaa attgcccttg ctcctgagcg tgatggtgtc
    23161 aaagaatttg tgtccgcagt gacaaaacaa ggcgtaacag tggccttagg gcattcaaac
    23221 ggtacttatc aagaggctaa agaagctgtt caagctggtg ctagtgtttg ggttcatgct
    23281 tataatggca tgcgaggatt gacccaccgt gaaccaggaa tggtgggagc ggtttataat
    23341 cttccaaata cttacgcaga gttgatttgt gatggccacc atgtctcacc aattgcttgt
    23401 gatattttga tgcagcaaaa agggcatgat catgtagcca tgattacgga ctgtatgcga
    23461 gcaggtggtt caccagatgg tgattacctc ttgggggaat tctctgtggt tgtggcaaat
    23521 ggcactgctc gtcttaaaga gagtggaaac ttggcaggat ctattttgaa attaaaagac
    23581 ggccttagaa atgtagtggc ctggggaatc gcaactccgg cagaagccat tcatatggca
    23641 acctatgtgc cagctgtttc tgtcggtatt gacgacgtat gtggtcaaat caaagctggg
    23701 catgcggctg actttattgt tcttgataag gatttaacac ttgttgcaac ctatttaaat
    23761 gggcaaaaag cttttgatgc ctaagagata caaaaggatt ggggtagcta tgtctggcag
    23821 gccaattctt tttgtatttt ctttctttat gatagaatag attacagtaa tcgcttacag
    23881 agattgataa aaggaggttt ttgtgatggt aacaacagta aaaatgacaa gtggctatga
    23941 gattcccgtt ttaggatttg gaacttacca agcagccgat ggtgaggaag cttaccaatc
    24001 taccttagca gccatcaagg ctggctatcg tcatattgac actgcagcta tttataaaaa
    24061 tgaagaaagt gtgggtcgtg ccatcaagga ttctggggtt ctacgagaag acttatttat
    24121 tacaactaaa ctctggaatg acgcccatag ttatgaaggg gcaaaagacg ctttagctgc
    24181 ttctcttgac cgtcttggtc tggattatgt ggatctctat cttatccatt ggccaaaccc
    24241 aaaagctctg cgtaacactt ggaaagaggc aaatgctcaa gcatggcaat atatggaaga
    24301 ggcggttgaa gcagggctca ttaaaagcat tggcgtcagc aattttatgg tgcatcatct
    24361 agaagctctt caagagacag ctaaaataac tccagcaatt aatcagatcc gattagcccc
    24421 aggttgctat caaaaggaag tggttgatta ttgtaaggca aatgagattt tattggaagc
    24481 ttggagtcct ctaggacaag gggagatttt tgataatgag actatgcagc agttagctaa
    24541 taaatatgac aaaacagtgg cacaagctgc cttagcttgg tcgctggcag aaggttttat
    24601 tcccttgcca aaatcggtgc atgatgagcg catcaaggaa aatatggcta tttttgatgt
    24661 gagcttgaca ccagaagata aaaaaacaat tcgctatctc tcaggaatgt cagcgattcc
    24721 taaccctgat actacaagtt tttaagaaac tacaaagatt tagccataat tcgctaaatc
    24781 ttttttgaaa acggtttgac aaaggtttat catttagcta taatctttat tagtgttttg
    24841 tgaaagaaac tattattgaa aataaggagc cgtattgtga ccatcttttt agtgatgtta
    24901 gccatgttta ctattcgttt agtatttttg aagaaatcga taacaaatga aaaagccata
    24961 ttagcccaag gaggacagga atttggtgct cagaacacga aatttttgac gttacttcac
    25021 attatgattt atgtgtttgc ggtgattgaa gcgctgctta aacaaattaa gtttgatggc
    25081 atcagttttt taggtctttt gttgatgtta ttatcagtag ctgttttata tgaagtgaca
    25141 cgtatcttag gtgacatttg gactgtaaaa ttgatgttag caaaagacca caaatatgtg
    25201 gatcactggt tgttcaaaac gattaaacat cctaactact ttttaaatat cgcccctgag
    25261 ctggtgggca tagccttact ttgccatgct aaaattacgg ctatgcttct tttcccatgc
    25321 tacattgtag taatatattt acgtattcga gaagaaaata agctattagc ggaagtgatt
    25381 attccaaacg gtactcgaac aaaaccttaa gctcatgatg gacaaactaa tggctttatg
    25441 ctaaactaga ctctaggaaa aaggatgcaa gtatcttatc taaagcgagt tcggggtagt
    25501 tagagccgaa tggtaggact gcagattggc gcttccgttt gggcagtgtg attaagtata
    25561 tttgtcaata ttgcccaaaa agatactata taaatgaagt aataaattag ggtggaaccg
    25621 cgttttgacg cccctaggtc ttatgattta ggagcatcaa aatgcggttt tttggtgcct
    25681 agacctgtaa tacatgctag taacatcgtc aggtatttta ctgatgacaa aaaaacgatt
    25741 gtcaatgata taccatttta ctactatcaa aggagtacga aatgtctaaa aaacttacct
    25801 ttcaagaaat catcttgacc ttacaacaat attggaatga tcagggttgt atgctcatgc
    25861 aagcctatga caatgaaaaa ggagctggga ccatgagtcc ttacacgttc ttacgtgcta
    25921 taggtcctga gccatggaat gcggcttatg tagagccatc acgtcgtcct gcagatggcc
    25981 gttacggaga aaacccaaat cgtctttacc aacaccacca atttcaagtg gtcatgaaac
    26041 catcaccatc aaatattcaa gaactttacc ttgcttcttt ggaaaaattg ggcatcaatc
    26101 ctttagaaca tgatattcgt tttgttgaag ataactggga aaacccatca acaggatcag
    26161 cagggcttgg ttgggaagtt tggcttgatg gaatggaaat cacacaattt acgtatttcc
    26221 aacaagttgg tggccttgcg acatctccag tgacagcaga agtaacttat ggtcttgagc
    26281 gcttagcttc ctatattcaa gaagtggatt cggtctatga tattgagtgg gcaccaggtg
    26341 ttaaatatgg tgaaatcttc ttacaacctg agtatgagca ttcaaaatat tccttcgaaa
    26401 tatcagacca agacatgctg cttgaaaact ttgaaaagtt tgaaaaagaa gcctcgcgtg
    26461 ccctagaaga aggtttggtt cacccagctt atgattacgt gctcaaatgt tctcatacgt
    26521 ttaacctgct ggatgcccgt ggcgctgttt ccgtcacaga acgcgcaggc tacattgctc
    26581 gtatccgtaa tttggcacgt gtcgttgcta agacctttgt tgcagaacgc aagaagcttg
    26641 gattgccact gttggatgaa gcaacacgcg caatcttgct agctgaagat gacgaataag
    26701 tcgatttaaa gatgatctag ttgcgagctt tgtaatctta agagcgagca agaagtgggc
    26761 gaccgaaggt cgccgagtca aggttactcc cgctgtagtg acaattcggt aaggttactt
    26821 atcgaacgag ggagttggcg agactttagg ctggctaact aggaatggaa cacttttgtt
    26881 ttctgaaatt atccactgga taatttcact ttgcgtcccc cctacctaag ggaacaatca
    26941 aaaagatact ttttagagaa aggaactgaa catgggctac ggactatgtc aaaaagacca
    27001 agacttccta ggtgcaagca cctgcgtcgc tttgctattt tgatggtgtc cgcttaacgc
    27061 ccttggtatc ttaattatga gtaaaaatct actaatcgaa cttggtctgg aagaacttcc
    27121 agcctacgtg gtcacaccaa gtgaaaaaca actgggagag cgcttggcga ctttcttaac
    27181 agaaaatcgt ctctcttttg aagatataca aaccttttca actccacgcc gtttggcagt
    27241 gcgtgtgtct ggattagctg atcagcaaac ggatttgact gaggacttta aagggcctgc
    27301 taagaaaatt gcccttgatg ctgatgggaa tttctcaaaa gctgctcaag gatttgttcg
    27361 tggcaaaggc ttgaccacag atgctatcga attccgcgaa gtcaaaggag aagaatacgt
    27421 ttatgtgacc aaacacgaag ctggaaaacc tgctaaggaa gtgcttctag gcgttactga
    27481 agtgttgtca gcaatgacct tcccagtcag catgcactgg gctaacaata gttttgaata
    27541 tattcgtcct gttcacacct tgacagttct gttgaatgat gaggccttgg aacttgactt
    27601 cttggatatt cattctggac gcgtcagccg tggtcaccgt tttttaggta ctgaaaccac
    27661 tatcacaagt gctgattctt atgaggctga tttgcgcagc caatgcgtca ttgttgacgc
    27721 taaagagcgt caagagatga ttgtggagca aatcaagacg cttgaagtgg agcagggtgt
    27781 gcaagttgac attgacgaag acttgcttaa tgaagttttg aacttggtcg agtttccaac
    27841 agctttcatg ggaaattttg aggctaaata ccttgatgtc ccagaagaag tattggtaac
    27901 atcaatgaaa aaccaccaac gttattttgt ggtacgtgat caggctggtc acttgatgcc
    27961 aaactttgta tcagttcgta atgggaatga tcaagccatt gaaaatgtta ttaaagggaa
    28021 cgaaaaagtt ttggtagctc gcttagaaga tggtgaattc ttctggcgtg aggatcagaa
    28081 attgcaaatt gctgatttag ttgccaaatt gaccaatgtg accttccatg aaaaaatcgg
    28141 ctctcttgct gaacacatgg atcgtacgcg tgtcattgcc gcatcacttg ctaaggaagc
    28201 caacctatct gctgaggaag tcacagctgt tgaccgtgct gcccaaattt acaaatttga
    28261 ccttttaaca ggtatggttg gtgaatttga tgagctgcaa gggattatgg gtgaaaaata
    28321 tgctcttcta gctggtgaag atgctgctgt ggcaacagca atccgtgaac actacttacc
    28381 tgatgctgca ggaggtgcgc tacctgaaac caaagttggc gttgtcctgg ccttggcaga
    28441 taaattggat actctcttgt cattcttttc tgttggattg attccatctg gctctaatga
    28501 tccttatgct ctacgtcgtg caacgcaggg tatcgttcgt attcttgatc attttggatg
    28561 gcgcatccca atggataagc tggtagatag cttatatgac ttgtcctttg atagtttgac
    28621 ctataccaat aaggcggatg tgatgaactt tatccgtgct cgtgtggata agatgatggg
    28681 caaagccgca ccaaaagaca ttcgggaagc tattctagca agttcaacct ttgtggttcc
    28741 agaaatgtta gcggtagctg aagctcttgt caaagccagc catacggaaa attacaaacc
    28801 agcagttgaa tctttgtcac gtgcttttaa tttggcagaa aaagcggacg cttctgttca
    28861 ggtggaccct agcctctttg aaaatgagca agaaaacact ttgtttgcag ctattcaagg
    28921 cttaactcta gcaggcagtg ctgcccagca gttagaacag gtctttgccc ttagcccagt
    28981 gattaatgat ttctttgata atacaatggt tatggcagaa gatcaagccc ttaagaacaa
    29041 ccgtgtagct attttatcag atttagtaag caaagctaag accattgctg cttttaacca
    29101 actaaacacc aagtaaatag aaaggaaaga gccgttgggg acttagctag aaaagtgagt
    29161 tcagtacccg agtggaaaga acaaggcctt taaacattgc ttttcacagt gagagtcaag
    29221 tcagtttttt aagtcttaca cttttttccc aggctcttag tggcttaaca tggatcctaa
    29281 aaaaattgct cgtatcaatg agctggctaa aaagaaaaaa acggtaggct tgactggacc
    29341 tgaaaaagtg gaacaggcaa aactccgtga agaatacatt gaaggctatc gtcgctctgt
    29401 cagacaccat atcgaaggca tcaaattggt ggacgaagaa ggaaacgacg tgacccctga
    29461 aaaattaaga caggtgcaac gtgaaaaagg cttgcacggc cgttcattag atgacccaaa
    29521 atcataagac aaaaagcagc tctgaactgc tttttgttta tcgtgatgac tgttagcttt
    29581 tattttaaat ggtatgatac ttcaaagtga ccacacgttt ttatattgta tagtcaaaat
    29641 attgggaaaa accatgtgtt tgagttataa tagaagaaat caaagaaaga aggtagtact
    29701 tatgacaatt aaaaaattct ctctactagc catagccagc ctctctttac tcagtcttgc
    29761 agcctgtgat atggatgata aagacgatca tatggataat caaccaaaga caagtcaaac
    29821 tagcaagaaa gttaaacttt cagaagacaa agccaaatca attgcgttaa aagatgcttc
    29881 tgttacggaa gctgatgctc aaatgctatc agttacacaa gataatgaag atggcaaagc
    29941 cgtgtatgaa atcgagtttc aaaacaaaga tcaagaatac agctatacca ttgacgccaa
    30001 ctcaggggat attgtggaaa aatcttctga accaatcaat gactaatcaa gaactgtaat
    30061 gaataagaca gccttaactc ttgtgtccaa ttgtctttgc ctcttaggcg cggctgtttg
    30121 ataccgtgtt gcctttgagg cttagttctc tttatgaccc aatgatgact ctggattagc
    30181 agagtgattg ttgggtcttt tgattttctt tgataagaaa gattacctcg ttagaaatga
    30241 ggagacgtgg ttgtcatcag tggatttttc aagatcatct agtgggataa gctacctatc
    30301 ttatcagctt ttttttagct ttaaaaattc ttgataatat atttctgata aaattagcta
    30361 taatagaatc aaataggaaa acgcttactt aaaggagggt aatgaatgtc gcaagaaaag
    30421 tatatcatgg ctattgatca gggaacaaca agttcacgag cgattatttt caatcaaaaa
    30481 ggggaaaagg ttagcagtag ccaaaaagaa ttccctcaga ttttcccaca cgctggttgg
    30541 gtagagcaca atgctaatca gatttggaat tcggttcagt ctgttattgc gggtgctttt
    30601 attgagtcca gcatcaaacc aagccaaatt gaagcaatcg gaattaccaa ccaacgtgag
    30661 accacagtgg tttgggacaa aaaaacgggt gtacctattt ataatgctat tgtgtggcaa
    30721 tcacgacaga cggcaccaat tgctgagcaa ctcaaacaag atggccatac aaaaatgatc
    30781 catgaaaaga caggccttgt tattgatgcc tacttttctg caaccaagat ccgttggatt
    30841 ttagatcatg ttcctggtgc tcaagaacga gctgaaaaag gcgagttact ttttggaacc
    30901 attgatactt ggctggtttg gaaattgaca gatggtgctg ttcatgtgac ggattattcc
    30961 aatgcggctc gtaccatgct ctataatatt aaagacttaa catgggatga tgagatttta
    31021 gagcttttaa atattccaaa agatatgctt ccagaggtta aatccaattc tgaaatttat
    31081 ggcaaaacag ctgcctttca tttttatggg ggcgaagtgc ctatttcagg tatggcgggt
    31141 gaccaacagg cagctttgtt tggccaacta gcttttgagc ctggtatggt gaaaaacact
    31201 tatggcacag gttctttcat tatcatgaat actggtgatg agatgcagtt atcgagtaat
    31261 aatcttttga ccaccattgg ttacgggatt aatgggaagg ttcactatgc cttggaaggt
    31321 tccatcttta ttgcaggctc tgccattcaa tggttgcgag atggtcttaa aatgattgag
    31381 acttcaccag aatctgaaca gttcgctcta gcatcaacta gtgacgatga agtttatgtt
    31441 gtgcctgctt ttaccggttt aggagctcct tactgggatt ctaatgcgcg tggatctgtc
    31501 tttggcttga cacgtggtac cagcaaagaa gactttgtca aagcaacctt acaatctatc
    31561 gcctatcaag tgcgagatgt tattgatacc atgcaggtgg attcaggtat tgacattcaa
    31621 cagctccgtg tggatggcgg tgcagccatg aataatatgc tcatgcaatt tcaggctgat
    31681 attttaggaa ttgatattgc ccgtgctaaa aatttagaaa caacagctct aggagcagct
    31741 ttcttagcag ggcttgctgt ggggtactgg gaagacatgg atgctttgaa agagttaaat
    31801 gccacaggcc agctctttaa ggctagcatg aatgaatcgc gcaaagaaaa attatacaag
    31861 ggttggaagc gcgctgttaa agcaactcaa gttttcaccc aagaagaaga tgcagatgac
    31921 gatgcaaaat aactagagga ggatggaatg gaattttcaa aagagacaag acgcttagct
    31981 ctccaaaaaa tgcaagaaag agatttagac ctactgatta ttgggggagg tattacgggt
    32041 gctggtgtgg cgcttcaggc ggcagctagt ggcctagata cgggtctgat tgagatgcaa
    32101 gattttgcgc aaggaacctc tagccgttca accaaattgg ttcacggggg gcttcgttac
    32161 ttgaaacaat ttgatgtgga ggtagtttca gatacggtgt cagagcgggc tgtggtgcaa
    32221 caaattgcac cccacattcc aaaaccagac cctatgttat tacctgttta tgacgaacct
    32281 ggcagcacct ttagcatgtt ccgtttgaag gtcgctatgg atttgtatga tcttttagca
    32341 ggcgtgtcca atacgccagc ggccaacaag gtgttaacca aagaagaagt cttaaaacga
    32401 gaaccagact taaaacaaga aggcttgctt ggtggtgggg tttaccttga tttccgcaat
    32461 aatgacgcaa ggcttgttat tgaaaatatc aaacgagcta atcgtgatgg ggctcttgtt
    32521 gctagtcatg tgaaagcaga agatttcttg ctagatgaca atggtaagat tattggtgtg
    32581 aaggcgcgtg atctgctgtc agatcaagaa atcattatca aggctaaatt agtcatcaac
    32641 accacaggtc catggagtga tgagattcgt caattctctc ataagggaca accgattcat
    32701 caaatgcgcc ctacaaaagg ggtgcatctg gtagtggacc gtcaaaaatt accagtgtct
    32761 caacctgtct atgttgacac agggttaaat gatggtcgta tggtctttgt cttgccacgt
    32821 gaagaaaaaa cttattttgg aacaacggac acggactaca ctggagactt ggagcaccca
    32881 caagttactc aagaagatgt ggattatttg ttaggcgttg tcaataaccg ctttccaaat
    32941 gccaacgtga ccattgatga tattgaaagc agttgggctg gtcttcgccc cttgttatca
    33001 ggtaatagtg cttctgacta caatggtggc aacagcggta aagtcagtga tgatagtttt
    33061 aatcacttgg ttgatactgt caaagcctat attaaccacg aagatagccg agaagctgtt
    33121 gaaaaagcta ttaagcaggt tgaaaccagc acatctgaaa aagaattgga tccgtctgca
    33181 gtgtcacgag gttcaagttt tgaccgtgat gagaatggac tctttacctt ggcaggtggt
    33241 aagattaccg actatcgcaa aatggctgaa ggagcattga cagggattat tcaaatcctc
    33301 aaagaagagt ttggcaaatc cttcaagctt atcaattcaa aaacctatcc tgtttcagga
    33361 ggtgaaatca atccagccaa tgtagattca gaaatagaag cttatgctca attaggaact
    33421 cttagtggtc tttcgatgga tgatgctagg tatttggcaa acctttatgg ttctaatgcg
    33481 ccaaaagtct ttgccttaac tcgtcaatta acagcagctg aagggttaag tttagctgaa
    33541 accttgtcct tacattatgc gatggattat gaaatggctc ttaaaccgac agattatttc
    33601 ttgagaagaa caaatcacct cttatttatg cgagatagcc tagatgcttt gattgaccca
    33661 gtgattaatg aaatggctaa acattttgaa tggtctgatc aggaaagagt ggcacaagaa
    33721 gacgatcttc gtcgcgtgat tgcagacaat gatttgagtg ccctaaaagg ccagcaggag
    33781 ggttaatatg gatatctttg gtgaattttt gggtacagcc ttattggttt tactaggaaa
    33841 tggtgtggta gcaggggttg tcttacccaa aacgaaaacc catgcttctg gctggatcgt
    33901 gattgcgact ggctggggaa ttgcagttgc tgtggcagtt tttatcagtg gcaaagttgc
    33961 ccctgcccac ctcaatccag ctgttagcct tgcttttgcc atgagtggaa ccattgcctg
    34021 gtcaacagcc attgcctata gccttgccca actattggga gctatggttg gttcaactct
    34081 ggtattcctt cagttcaggc cacattatct ggctgctgag agtcaggctg atattttagg
    34141 gacatttgcg acaggtcctg ctattcgaga taccagctca aacctattga gtgaaatctt
    34201 tgggaccttt gtcttgatgc ttggtatttt ggcatttggc ttatatgata tgccagcagg
    34261 actaggaacc ctctgtgtag gtaccttggt tattgggatt gggttatcct taggaggaac
    34321 gacaggttac gccattaacc cagcgcgtga tttaggacct cgtctagttc atgctattct
    34381 accactcaac aacaagggag attctgattg gtcttatgct tggatacctg ttgtaggacc
    34441 aattattgga gcggccttag cagttttgct tttccaagtc atgtcctaac catcactaaa
    34501 ggggttggct atcataggcc agctcctttg ctttttcttt ataggattag gtaaaacgct
    34561 ttcatatctt aaaaacaact gctataataa tactattatg acattaacga caaagaccat
    34621 acatgttatc ggggcatcct ttgctggtct agcctttgtt gataaatata aggacctcaa
    34681 tccagattcc caaatcattc tcattgataa ggaaagctgt cccaattata ttccaaatgg
    34741 tatcaaccaa ttattcagag gagacattca agacctgtct gatgctatgt ggggaagggc
    34801 ctgcctagca gcacaaatag aatctaacca ccggtttatt caagcagaag tgttagctat
    34861 cgaagctccc tcaaacacct tgcttctcaa agacagtcaa ggacgagttt ttgaagaagg
    34921 ttatgaaacg cttgtttgtg ccatgggtgc tagcccccag tctcattata ttgagacgtc
    34981 acagaccaac aaagttttgg taaccaaata ttatgaagaa agccaagcta gtttaaaact
    35041 gattgaggct agccaagagg tcttggttat tggtgccggc ttgattggct tagaccttgc
    35101 ctacagtttg tccttacaag gcaaacgggt caaactgatt gaagcagccg aacgtccaga
    35161 tttctatcag actgacgcag aactgatagc ccctgtaatg gcagaaatgt ccactcacca
    35221 tgtcaccttt attaataata agcgtgttac ggctatccat gagatagagg gaaaagttgt
    35281 cgctcatacc gagcaagggg acaccttcca aggtgatttg gctattttag ccattaactt
    35341 taggccaaat acacaccttt tgcaaggaca agtggcttgt gctctggata agaccatttt
    35401 ggtcaatgaa aacttacaga ccagccaggc taatatctat gccattggag acatggtttc
    35461 attgcatttt ggtatattgg gaatggatta ctatacacct ttgattaacc aagcaatgaa
    35521 gacaggtcag gctctggctc tgcatttagc tggatatcct attcctccat tacagacggt
    35581 caaggtatta ggaagttctc actttggtta ttatcgcgcg agtgttggtg tgacagaaga
    35641 agaagcagag ctttatatgg atacgtgtag ctatttgtac caaaatgggg attctaaaaa
    35701 cctcttttgg ctgaaattaa tagctagaaa aacggatggg attttaatag gcgctcagct
    35761 cctatccaaa accaatgctc ttgtcatcgc caatcaatta ggtcaggctc tggctctgaa
    35821 agttacagac ggtgacttag catttcaaga ttttctcttt ttacaaggtc acagtgattt
    35881 ggcttatcat cttcatgaag cttgtctcaa attatttgaa aagaggctcc gccatgaaga
    35941 ttgaagactt aatggataaa gaacgaaaag ctcaatatcg tctcttggtg accctttatc
    36001 atgctaaaga aacgttaagg ctaaaagacc taatgaggct atcaaacttg tctaaagtga
    36061 ccctattaaa atacattgac aacctaaatc atttgtgtcg agaacaaggt ttagcctgtc
    36121 aattactttt agaaaaagac agtctgagct taaaagaaaa tggccagttc cattgggagg
    36181 atttggtagc tctgcttctc aaagaatcgg ttgcctatca gatattgacc tacatgtact
    36241 gccatgaaca ctttaacatc accaacttat ccgttgaact gatggttagt gaagctactc
    36301 taaacagaca actagctcac ctcaaccagc ttttatcaga atttgatctg gcgctctcgc
    36361 aaggcagaca actgggaagt gagcttcagt ggcgttattt ctatttggaa ttgttccgtc
    36421 ataccttgac cagacaaggc atagacgcct tggtcaatca gttagacgct tcgcatttgg
    36481 ctaccttaat tgagcggctg attggccagt cattatcggc agaagcctta gaacaattat
    36541 tgatttggtt ggcgatttcg caagcgcgaa tgtcctttca aaaaggctac aatgatcatt
    36601 ttttaagaga tagtgacttt atgacatcta atattttctt taagcgctta gaaagcatgc
    36661 ttttacatta cttaagacgc tatgcccttg aatttgatgc ttttgaagct aagagcctct
    36721 ttgtcttttt gcatgcttat cctctacttc ctattgccag catgaagtat agtttgggtt
    36781 ttggaggtcc tattgctgat cacatttcag aagccttatg gcttttaaag aaggctcatg
    36841 tgattatcca tcagacaaag gaggaaatca tttatggctt ggggatattt ttttccaaag
    36901 cttacttttt taaaggagct atccttagcc aaccaaccaa tagccaatac ctttatcaat
    36961 tagtaggcga agataaaaga gcgcttttaa gggttatcat aaaccattta gtgctgcaaa
    37021 tgggtcagga aacggatttc agtcagcagt tgagtgacga cattttagca ttgttggttt
    37081 tttccattga gcgccaccat gagcctcttt tagtagggct tgccttgggg caaaataagg
    37141 tggaggcagc cattgcggaa ttggccatta gacgtcattt ggggcatcgt agggattttc
    37201 aattaatgcc ctatgatcat caaaaagttt atgactgtct gattacctat cagactgtct
    37261 atttaccgag acaagattta ccctattacc ggttaaaaca gtatagctcc ccctatgaac
    37321 tcactgcctt agaagctttt ctcaaagacc ttttccagca aaaaaataca cgagaagagg
    37381 aactctcact cagcccagca gctaagtcta gttttgcaca caaaactgtg tgatacttgg
    37441 tcttttctaa agactgttac acttttataa taataaagta ggatagtcta aaggtagatg
    37501 atttgaaaaa gcttatcaaa tagagcttaa caaaatcgaa aatttttgga ggtaagaaat
    37561 gaaatttttc ctagatacag ctaatgtagc cgctattaaa gcgattaatg aacttggcgt
    37621 tgtagatggt gtcaccacca atccaaccat tatctcacgt gaaggacgtg attttgaaac
    37681 agttatcaaa gaaatctgtg acatcgtcga tggcccaatc agtgctgaag tgacagggtt
    37741 aacagcagat gctatggtag aagaagctag aagcattgct aagtggcatg acaatgtcgt
    37801 cgttaaaatt ccaatgacca cagaagggtt aaaagcaaca aacattcttt caaaagaagg
    37861 gattaaaact aatgtgacct tgattttcac agtaagccaa ggtttaatgg caatgaaagc
    37921 aggcgcaact tacattagcc catttatcgg tcgtttggaa gatattggca cagatgctta
    37981 tcaattaatc agcgaccttc gtgaaatcat tgacttgtac gatttccaag cagaaatcat
    38041 cgcagcaagt attcgtacaa cagcccatgt ggaagctgtt gctaaattag gcgcacacat
    38101 tgcgacgatt ccagacccat tatttgctaa gatgacgcaa catcccttaa cgacaaatgg
    38161 attgaaaaca tttatggaag attgggcttc ctttaaaaaa taaggctaaa gagtctgtgg
    38221 ccactgtgtc aacaggctct cttatattca tcgtcaaaaa gaattcacca atgatgtcca
    38281 aactagtttt tttctggcaa aatagagaaa aagaatttag ggactttgga ggattttctg
    38341 aaaaaagcgt ctatttttgt gatacaatag acaatagaaa acgtttaatt ttagttgtaa
    38401 taaacagaga ggttttactt atgacatttg atgcaattga ccagttggca gtaaacactg
    38461 tccgcaccct atcaatggat gccattcaag cggcaaattc tggacaccca ggacttccaa
    38521 tgggggcagc acctatggcc tatgttcttt ggaatcactt catgaacatc aatcccaaaa
    38581 caagccgtaa ttggtcaaac agagaccgtt ttatcctatc agcaggtcat ggaagtgcca
    38641 tgctttatag cttgttgcat ttagcaggtt atgatttatc tgtagaagat ttaaagaact
    38701 tccgtcaatg ggcttctaaa acaccaggtc acccagaagt gaaccacaca gacggtgtcg
    38761 aagcaaccac aggacctctt ggtcaaggga tcgcaaatgc cgttggtatg gccatggcag
    38821 aagctcacct agcagctaaa tttaacaaac caggctttga catcgttgat cactacacat
    38881 ttgctttgaa tggtgacggt gaccttatgg aaggggtcag ccaagaagca gcaagtatgg
    38941 caggacattt aaaacttggg aaattggtct tgctatatga ttcaaacgac atctctcttg
    39001 atggtccaac ctctatggct ttcacagaag atgtgaaagg acgtttcgaa gcttacggtt
    39061 ggcaacatat ccttgtgaaa gatggaaatg atttagaaga aattgcagct gctattgaag
    39121 cagctaaagc tgaaactgag aaaccaacca tcatcgaagt caaaaccatt attggttttg
    39181 gtgccgaaaa acaaggaact tcagctgttc acggcgctcc tcttggagca gaaggcattg
    39241 cttttgccaa aaaagcttac caatggactc atcaagattt tgaagtgcct gctgaagtaa
    39301 cagagcgctt tgctcaaggc cttcaagcgc gtggtgaaaa agcagaacaa gcctggaatg
    39361 acttgtttgc agtttatgaa gcagaatatc cagaattagc agcagaatac caaaaagcct
    39421 ttactaacga agctgctcaa gtagaacttg aagcgcacga acttggtagt tcaatggcga
    39481 gtcgtgtatc cagtcaacaa gccatccaac aaatctcaga acaagtagcc tctttctggg
    39541 gtggatcagc agatctttca gcatctaaca acacaatggt taaggctgaa acagatttcc
    39601 aaccaggtca ctacgaaggc cgtaacatct ggtttggcgt tcgtgaattt gccatggctg
    39661 cagccatgaa cggtattgcc cttcacggcg ggacacgcgt ttacggcgga accttttttg
    39721 tcttctcaaa ctatcttctt ccagctgttc gtatggcagc gctccaaaac ttgccaacag
    39781 tttatgtcat gacacatgat tctatcgctg ttggtgaaga tgggccaacg cacgaaccaa
    39841 ttgaacaatt ggcaagtgtt cgctcaatgc ctaactttaa cgttattcgt ccagcagacg
    39901 gtaacgaaac aaatgctgct tggaaacgtg ccattgctga aacagatcgc ccaaccatgc
    39961 ttgtattgac gcgtcaaaac cttccagtgc ttgagggcac aaaagaattg gcagaagatg
    40021 gtcttaacaa aggagcttac atcttgtcag aagccaaagg cgaccttgaa ggtatcttga
    40081 ttgcaacagg ttcagaagtg aaactggcaa tggatactca agaagctctt gaagcagaag
    40141 gcattcatgt gcgtgtcgta tctatgccat cacaaaatat ctttgacgaa cagtcagcag
    40201 agtataaaga aagcatccta ccagcagctg tgactaaacg tctcgctatc gaagcaggtt
    40261 caagctttgg ctgggcaaaa tatgttggcc tagcaggaaa aacattgacc attgacactt
    40321 ggggtgcttc agctccagga aatcgtatct tcgaagaata tggtttcact gtggcaaatg
    40381 caactgagtt atacaaatca ctctaggtta cacatcccaa acagagtaaa tcaaatgatt
    40441 tactctgttt ttttaaaata aaataaccag ttctttcttt tcctcaaact gcctaataca
    40501 cttcttttga taagaagtca ttgacaaaac gattcataaa aattataatt taattgttat
    40561 tgtattagcg taaaagtaca aaaaataaag gagtataaat ggaaaagaaa cgacagcgct
    40621 tatacgatgt gattagacag gcttatgatt atccagaaaa ccgtgaaaat gtggccttgt
    40681 cgcagctact tttagcagct tctaatagat taattaaaca cagtaaccca cttcttgttg
    40741 cctaccaact aaaccaagat gtggataatt acttacttga taatgacatt ttacttccaa
    40801 aatcgctttg ccgttttaag cagtcattgg aaaagtatat tttagaggaa gcctcatcag
    40861 tctagggaga aaaagagaag aaaaggaaaa aggataccat gatagaattt aaacatgttt
    40921 caaagttgta tggcgacaaa gaagctctca gcgatttaaa cgtcaccatc aatgacggtg
    40981 aaatttttgg ccttattgga cataatggtg caggtaaaac cacgaccatc agcattttga
    41041 cttctattat cgaagcgagt tatggcgaag tatttgtgga tggtcaactt ctgacagaaa
    41101 atagagaagc tatcaaaaaa caaattgctt atgtgccaga ttctcctgat attttcttga
    41161 atctaactcc aaatgagtat tggcagtttt tagctaaaat ttatggggtg tcagatgagg
    41221 accgagaaga acggttagca cagctgacaa cactttttga actgaaggaa gaagttaatc
    41281 aaaccattga tagtttctca catgggatgc gccaaaaagt gattgtgatt ggtgctcttg
    41341 tatcaaatcc taacatctgg attttggatg aaccattaac aggtttggat cctcaggctt
    41401 cttttgacct taaggaaatg atgaaagctc atgcagcttc tggacatacg gtcttatttt
    41461 cgactcacgt gctatcagtc gctgagcagt tatgtgatcg tatcgggatt ctaaaaaaag
    41521 ggaaattgat ttttgttgga acgattgatg agttgaaaga acaccatcct gacaaagact
    41581 tggagagcat ctaccttgaa ctcgcaggtc gtaaggcgca agaagagggg tgatgggatg
    41641 aattggtcta ctatttggga acttatcaag ataaacattc tttattccaa tccacaaagt
    41701 ttagctaacc tcaaaaagcg tcaggaaaac cacccgaaag aaaacttcaa agcctataaa
    41761 agcatgatga ggcaacaggc cttaatgatt gccatgtttt tggtgattta cctttttatg
    41821 tttattggtg tcgatttcag tcattatcca ggacttttct cctttgacgt tgctatgttt
    41881 tttatcatgt caaccttgac agcctttagc tctctttaca ccatttttta tgaaagtaat
    41941 gacttaaaac tttatattca cttaccagta acgtcagaag aactctatat tgcaaaaatt
    42001 gtctcgtcgc tagggatggg tgctgtcttt ttgatgcctc ttatctctct tctcttaatt
    42061 gcctattggc aactattggg aaatcctttg tctattttag tagctattgt gctcttttta
    42121 gtgttactag tgagttcgat ggtattagct atttatatca atgcttgggt gggcaaaatc
    42181 attgtaagga gtcgcaagcg gaaactcatt tcaactatca tgatgtttgt ctcaactttt
    42241 ggagctttcg tcttaatttt tgcgattaat attagtaaca acaagcgtac gatgacggat
    42301 ggtgtattta ctgattaccc aactattccg tatttcaaag gattctatga tgttattcag
    42361 gcaccgtttt ctactgcggc cctccttaac ttttggttgc cattgctact gattttagct
    42421 atggtatatg gtattgtcac aaaagtgatg ccaacttatt atcgtgaggc tttttatatt
    42481 agtaacgaga acaaagccaa gcaaactaaa aaaccagtta atcgtcctca tcagaatcaa
    42541 tcactgacgc agttgttgcg aaaacatcat cttttaacgt tacaaaatgc aactttactg
    42601 acacaaacct atctcatgcc cctgatgtat gtgatgcttt ttatcggtcc aagtttgtca
    42661 cgtggcacag gtttctttaa gcatatttcc ccagattact ttggggtggc cttattattt
    42721 ggggtcagtt tgggtgtcat gtgtgcaaca ccaaccagct ttattggagt aggtatttca
    42781 cttgaaaagg ataactttac ctttattaaa agcttaccaa taacgttaaa aaaattcttg
    42841 ctggataaat tttgcttgct cgtcggtctg caactgattg tgcctatggt gatttatctt
    42901 gtgtttggtc tctttgtgtt gcacctgcat cctcttctaa cgattgcttt ttgtcttggc
    42961 tacgccctgt ctctgattgt gcaaggggaa ttgatgtatc gtcgtgacta ccggcttctg
    43021 gatttaaaat ggcaagacat gacacaactc tttaccagag gaaatgggca gtggctaaca
    43081 atgggcctta tctttggtaa tttaatagtc acaggtgtag taggttttgg agctgttatc
    43141 attgccaata ttatccaaca acctctgttg attagtattc tattgagttg tctgacacta
    43201 atggttttag ggattgctca cttatggatt caaaaaacct tctggaaaag cttagagaag
    43261 ctttaacgaa gagagatcat tattcggaaa aacagtcctc tggggctgtt tttatgctat
    43321 aatttgggta tgatgaaacg acaatttgaa gatgtaacac gtatcgtaat taaaattggc
    43381 actagttcat tagtgctacc aacaggtaaa atcaatttgg aaaaaatcga ccaactggct
    43441 tttgtgattt caagtttgat gaataagggc aaggaagtta ttttagtatc atcgggagcg
    43501 atgggatttg gactagatat tttgaaaatg gaaaaacgtc cgaccaatct tgctaaacaa
    43561 caggcagttt ccagtgttgg tcaggtggcc atgatgagtc tttattcaca aatttttgca
    43621 cactaccaaa ccaacgtgtc acaaatttta ttgacgagag atgtggtggt tttcccagaa
    43681 agtctagcca atgttaccaa tgcttttgaa agtctgatta gtttgggcat tgtgccgatt
    43741 gttaatgaaa acgatgcggt tagtgtggat gaaatggatc atgcgactaa atttggagat
    43801 aatgaccgtt tatctgctgt cgttgctggt attacaaaag ctgatttatt aatcatgttg
    43861 tctgatattg atggcctttt tgataaaaac cctactattt atgaggatgc gcagttacgc
    43921 agtcatgttg cagtcattac gcaagaaatc attgcgtcag caggtggtgc gggcagtaaa
    43981 tttggcacag gtgggatgct tagcaaaatt cagtctgctc agatggtatt tgaaaacaaa
    44041 ggacagatgg tcttgatgaa tggagctaat cccagagata ttttaagagt gcttgaaggg
    44101 caaccactag gcacctggtt caagcagata gaagaggtaa cacatgactg atatgagaag
    44161 gttagggcaa agggcaaaac aagctagtct tttaatagca cctttgtcga cgcaaattaa
    44221 aaatcgcttt ttaagcacct tagcaaaggc attagttgac gatacacaga ctctcttagc
    44281 cgctaaccaa aaagatttag cgaatgctaa agaacatggt atttctgaca tcatgatgga
    44341 tcgcttgcgc ttaacttcag aacggattaa agcgatagca cagggagttc aacaggttgc
    44401 tgacttagct gatccaatcg gacaagtcat taagggctat actaacctcg atggccttaa
    44461 aatcctccaa aagagagttc ctctaggggt gattgcgatg atttttgaga gtcgcccgaa
    44521 tgtttcagtg gatgctttta gtctggcgtt caaaaccaat aatgccatca tcctaagggg
    44581 aggaaaagat gcccttcact caaataaagc tttggtgaag ttgatccgac aaagtctgga
    44641 aaaatcaggg atcaccccag atgctgtcca gctagttgag gatccctcac atgctgttgc
    44701 tgaagaattg atgcaggcaa cggactatgt ggatgtgttg attccacgtg gtggtgctaa
    44761 attaattcag accgttaagg aaaaagcaaa agtgcctgtc atcgaaacag gtgtggggaa
    44821 tgtgcatatc tatgtggatg ctcaagctga cttagatatg gctactaata ttgttatcaa
    44881 cgctaaaaca aagcgcccaa gtgtttgcaa tgcagctgag ggattggtaa ttcacgaagc
    44941 tgtagcagca aggtttattc ctatgcttga aaaagcgatc aatcaggttc agccagtaga
    45001 atggcgggca gatgacaagg ctttgccgtt atttgagcag gcagtgcctg caaaagcaga
    45061 ggattttgag accgaatttt tagattatat catgtctgtt aaggttgttt cctctttgga
    45121 agaagctatt tcttggataa atcagcatac tagtcaccat tccgaagcta tcatcacaag
    45181 ggatattaag gcagcagaga cctttcaaga tttagtggat gctgctgctg tttatgtcaa
    45241 tgcttcaaca cgatttaccg atggttttgt ttttgggctt ggtgctgaaa ttgggatttc
    45301 tactcaaaaa atgcacgccc gtgggccaat gggcttagaa gccttgacca gcactaaatt
    45361 ttatattaat ggggatggac atatcagaga ataagctact agaaggtccc ttaatgacta
    45421 atgaaagaga aaaagaagaa gcaaaggttg ctttagaaga acttttacat cagattcagg
    45481 tgattcattt ggaattgaaa attgtccgaa attggggcta ttttgatctt tttggagacg
    45541 gtatgcttgt cagctggatc aaacgtcgaa aaatgagcaa ggtcaaccaa ttattaatag
    45601 acttacaagt tactcttcaa aactgccgaa gatagctggc agacgtcgat aggctattat
    45661 tagctgactt accgaatatg agttgggata aaatctttga tatttggtta gacaacctgc
    45721 tgacagattg ttgtgtgatt aaagaagcta aaaatttttt acaagtagtt gaaattctag
    45781 aggcagcggc taagtcagtg cttgctaagt tggctgatag cgaacaaaaa gaacaagatt
    45841 agagaagagg aagattccca cgagaatgaa atccttttct cttacttttt catttctaaa
    45901 ccttttgaag tatggtacaa ttaaagttat gacaaaagaa tttcatcacg tgaccgtact
    45961 ccttcacgaa acagtggaca tgcttgacat aaagcctgat gggatttatg ttgatgcgac
    46021 gctaggtggc tcaggccact cagcttattt gttgtccaaa cttggtgaag aagggcacct
    46081 ctattgtttt gaccaagacc aaaaggctat tgacaatgca caagttaccc tcaaatctta
    46141 tattgacaaa ggacaggtaa cttttattaa agataatttt agacacctca aagcacgttt
    46201 aacagcgctt ggagttgatg aaattgatgg tatcttatac gaccttggtg tttccagccc
    46261 gcaattggat gaaagagaac gagggttttc ttataaacaa gatgctccat tggatatgcg
    46321 catggatcgt cagtcgctct taacagctta cgaagtggtg aatacctatc cattcaatga
    46381 tttggttaag attttcttca aatatggtga agataaattc tccaagcaga ttgctcgaaa
    46441 aattgaacaa gcaagagcta ttaagcctat tgagacaaca acagagttgg cagaattgat
    46501 taaggcagca aagccagcta aagagttgaa gaaaaaaggc caccctgcta aacagatttt
    46561 tcaagctatt cgcattgaag tcaatgatga attgggagcg gccgatgaat ctattcagga
    46621 cgctatggaa ttattagccc ttgatggtcg tatctcagtc attaccttcc attctctgga
    46681 agatcgccta accaagcagt tgtttaaaga agctagtacg gtggatgtgc caaaagggct
    46741 tcctctaatt cctgaagata tgaaacctaa gtttgaactt gtttcacgta agccgatctt
    46801 acctagtcat tcagagttaa cagctaataa aagggcacac tcagccaagc tacgtgttgc
    46861 caaaaaaatt cggaaataag cctatgacaa atgaaaaaag aactcaggca gttacaaatg
    46921 ccttgcaaaa acgaataaaa actttctcta gaattgagaa ggctttttat actgctatca
    46981 ttgtaacagc gattacgatg gcagtaagta ttatttacct gcaaagtcgt aagttgcagc
    47041 tacaacaaga aatcactagt ttaaatagtc atatttcgga tcaaaaacta gagcttaata
    47101 atgccaagca agaagtaaat gagttatctc gccgtgatcg tattatagat attgctggta
    47161 aagcaggctt aagcaaccgt aataataata tcaagaaagt cgagtaatct atgaaaaaat
    47221 ggcaaaaata tgttttagat tatgttgtgc gcgataggag aactccagtc gaaaatcgcg
    47281 ttcgagttgg acaaaatatg atgctcttaa ctatctttat tttctttatt ttcattatta
    47341 atttcatgat tattattgga acagatcaaa agtttggagt tagtttgtca gaaggggcga
    47401 agaaggttta tcaagaaacc gttacgatcc aagctaagcg tgggaccatt tatgatcgaa
    47461 atggtacagc tattgcagtg gattctacga cttatagcat atacgcaatt ttggataaat
    47521 catttgtctc ggcttcagat gaaaagttat atgtacaacc ttctcagtat gaaacagtag
    47581 ctgatatttt aaaaaagcat ctgggaatga aaaaaacaga tgtaattaaa cagcttaagc
    47641 gtaaaggact tttccaagtc tcgtttggac cgtcagggtc tggcatttca tatagtacta
    47701 tgtctactat tcaaaaggct atggaagatg ccaaaatcaa gggaattgct ttcacaacta
    47761 gtcctggtcg tatgtatcca aacgggacat ttgcttcaga atttataggc ttggcatctc
    47821 taacagaaga taaaaagaca ggtgttaaga gtttagttgg aaaaacaggt ctagaagctt
    47881 cttttgataa aattttatca ggtcaagatg gtgttattac ttatcaaaaa gatcgaaatg
    47941 ggaccacact cttgggtaca ggtaagactg tcaagaaagc tattgatggc aaagatattt
    48001 acacaacgct atctgagcct atccagacct tcttagaaac ccagatggat gtttttcaag
    48061 ccaaatcaaa tggtcagttg gccagtgcaa cacttgttaa tgctaaaact ggtgaaattt
    48121 tggcaacaac acaacgcccc acttataatg ctgatactct gaaaggactt gaaaatacga
    48181 actacaaatg gtacagtgca cttcatcaag gaaattttga gccaggctca accatgaaag
    48241 tgatgactct ggcagcggct attgatgata aagttttcaa cccaaacgaa acctttagca
    48301 atgctaatgg tttaacaatt gcagatgcta ctattcaaga ctggtctatt aatgaaggca
    48361 tttccacagg acagtacatg aattatgcac aaggatttgc cttctcaagt aacgttggga
    48421 tgactaaact tgaacaaaaa atgggtaatg caaaatggat gaattatttg acgaagttcc
    48481 gctttggttt tcctactcgt tttggtttaa aagatgaaga cgcaggtata tttccttctg
    48541 ataatatcgt gactcaagct atgagcgctt ttggtcaagg gatttctgta acccagattc
    48601 aaatgcttag agcctttact gctatttcta ataatggtga gatgttagag ccacaattta
    48661 tcagtcaaat ttatgatcct aacacagcaa gttttagaac ggcaaataaa gaaattgttg
    48721 gaaaacctgt atcaaaaaaa gccgctagtg aaacaagaca atacatgatt ggtgtaggaa
    48781 cagaccctga gtttggaaca ctctattcaa aaacatttgg accaattatt aaagtgggtg
    48841 atttacctgt tgctgttaaa tcaggaacag cacaaattgg ttcagaagat ggaagtggtt
    48901 atcaagatgg tggattgact aactatgtct attcggttgt ggcaatggtg ccagctgata
    48961 aaccagactt tttgatgtat gttactatga ctaaaccaca acattttggt cccctttttt
    49021 ggcaagatgt ggttaaccca gtattggaag aagcatactt aatgcaagat acactaacta
    49081 agccagtagt atcagatgct aatcgtcaaa caacttataa attaccaaac tttgtaggaa
    49141 agaatcctgg tgagacatca agcgagttgc gtcgaaatct tgtccagcca gttgtccttg
    49201 gtactggcag caagatcaaa aaagtatcgc atcagcccgg tcaaacgtta acagaaaacc
    49261 aacaagttct catattatca gaccgttttg tggaggtacc agacatgtat ggctggacaa
    49321 aatccaatgt taaaaccttt gctaaatgga ctggaataga catcagcttt aaaggaacag
    49381 attctggtcg tgttatgaaa caaagtgttg atgttggtaa gtccttgaaa aaaataaaaa
    49441 aaatgaccat tactttagga gattaacatg tttttaaccc ttattgcagc aatcatatcc
    49501 tttatggtat ctgctttcac aatgccttat tttatcaaat tctatcaatt gaaaaaaatt
    49561 ggtgggcaac aaatgcatga agatgtcaag caacatttag ctaaagctgg aacaccgact
    49621 atggggggaa ctgtttttct tttagtagct acggcagtat cgttattggt tagtctattt
    49681 tcgatcaaaa acactcaaag tttagctttg atttcaggca ttttatcaat tgttgtcatt
    49741 tacgggatta tcggattttt ggatgatttt ttaaaaattt ttaaacaaat caatgaagga
    49801 ttgacagcca aacaaaagtt agccttgcag ttggttggag gattgatgtt ctatttttta
    49861 catgttagtc caagtggcat ttcctctatt aatgtgtttg gttatcagct gccgctggga
    49921 attttctacc tattctttgt tttgttttgg gtggttggtt tttcaaatgc cgttaatttg
    49981 acagatggta tcgatggctt agcttctatc tcagtggtga ttagtctagt gacttacggc
    50041 gttattgcct acgttcagag tcaatttgat gttttgttac tgattggagc aatgattgga
    50101 gccttgcttg gcttcttctg ctttaatcac aaacctgcta aagtatttat gggagatgta
    50161 ggtagtttag cccttggagc tatgttggct gccatttcta ttgcgcttcg tcaagaatgg
    50221 actctcctga ttattgggat cgtttatgtt cttgaaacaa gttctgtgat gttgcaagtg
    50281 tcctatttca agtacaccaa gaaaaaatat ggagaaggtc gtcgtatttt tagaatgacg
    50341 cctttccacc accatttgga attgggaggc ttgtctggta aaggcaagaa gtggtcagaa
    50401 tggcaggtag atgctttttt gtggggagta ggaagtctag ctagtttact agttttagct
    50461 atcttatacg tgttctaagg gggattatca agaatagtca ccaatagcat tttgattaac
    50521 atcatcaata agagagtgga gatgacccac aaatgctgat tttgtgagtg ttgtcccact
    50581 ccttttgctt gtctctaaca ataatgatat aatatggaag aaaacgaggt aaaacatgtc
    50641 atttaaagat tatcatttta agcaatacgt tcagcaagcg ctggaagaaa ttggctttgt
    50701 caatccaaca gaggtccaga agcggctgat tcctattgtt aactccggtc gtgacttagt
    50761 tggggaatca aaaacaggtt cagggaaaac ccacaccttt ttgttgccca tttttgaaaa
    50821 acttgatgaa gctaaagcag aagtgcaagt cgttattaca gcacctagcc gtgaattagc
    50881 aactcaaatt tttgatgctt gtaaacagat tgctaagcac ttccaagaag agattcgttt
    50941 ggccaactat gttggaggca cagacaaact ccgtcagatt gaaaaactaa aaggcagcca
    51001 gccgcatatt gtcatcggaa ccccaggtcg tatttatgac ttggtcaagt ctggtgattt
    51061 agcgattcat aaagcaacga cctttgttgt ggatgaggcc gatatgacca tggacatggg
    51121 ctttttggat acggtagata agattgcagc ttcgcttcca aaatctgttc agattttggt
    51181 gttctcagca actattccac agaaattaca accctttttg aaaaaatact tgaccaatcc
    51241 tgttattgaa caaatcaaga caaagaccgt tatcgcagat accattgata actggcttgt
    51301 gtcgacaaaa gggcgtgata agaatggtca actgttagag attctcaaaa ccatgcagcc
    51361 ttatatggct atgctttttg tcaataccaa ggaacgcgca gatgatttgc atgccttttt
    51421 aacagctaat ggcttaaaag tggctaagat tcatgggggc attcctccaa gagaacgtaa
    51481 gcgcatcatg aaccaagtca aaaaactgga ctttgaatac attgttgcca cagacttagc
    51541 agcgcgtggg attgacattg aaggggtcag ccatgtgatc aatgacgcca tcccacaaga
    51601 cttgtctttc tttgtccatc gtgtaggccg aacaggtcga aatggtatgg caggaactgc
    51661 cattacactt taccagccaa gtgacgactc agatatcaaa gagctggaaa aaatggggat
    51721 tgcctttaca ccgaaggtgc tcaaaaatgg agaatttcaa gatacctatg accgtgatcg
    51781 ccgtcaaaac cgtgaaaaag cctatcaaaa attagacaca gaaatgattg gtttggtcaa
    51841 aaagaaaaag aaaaaagtca aaccaggtta caagaaaaaa attcagtggg cggttgatga
    51901 aaaacgtcgc aaagaacgtc gtgctgaaaa tcgtgctaag ggccgcgctg agcgtaaggc
    51961 caaaaaacaa catttttaag aacaaagagc aagcaagtga tcaattggca tctaatcttg
    52021 ctttttttac acctgctact tgtattgttt accatttgtt aacacaaaaa gtgatcatag
    52081 cttttgctta ttgctataat acggatttac tattttacat gtgggtctaa gagtgttaga
    52141 atattaagac aaacaaatac aaaggggaaa tacatgacat cagtgttttt aaccagtggt
    52201 tgggcttttt atgattacct catctcaccg attccacatg gaaaactttt tagttggcat
    52261 gctgtttttg atgccattcc aaatattatc caacggcttc caattacgct tggtttgaca
    52321 ctatcaggag caacctttgg cttggttttg gctttgattt ttgcccttgt caaaattaat
    52381 aaagtgaagc tactataccc tattcaagct atttttgtga gtttcttgcg aggaactcct
    52441 attttggtac agttgatgtt gacctactac ggtattcccc tctttctaaa atttctcaat
    52501 cagaagtatg gctttgattg gaatgttaat gcaattccgg cttctatttt tgccattaca
    52561 gcctttgctt ttaatgaagc agcctacgct agtgaaacta ttcgggcagc cattttatca
    52621 gtagatacag gtgaaattga agcagcaaaa agtctaggca tgacttctgt gcaggtttac
    52681 cgtcgtgtca ttattcctaa tgcaactgtt gtggctattc caaccttgat taatgggtta
    52741 attggcttaa ctaaaggaac atcgcttgcc tttaatgcag ggattgtcga aatgtttgcc
    52801 caggctcaaa ttctaggcgg atcagattac cgttattttg aacgttatat ctctgttgct
    52861 cttgtctatt ggtctatcag tattttgatg gagcaagtag gtcgcttgat tgaaaacaag
    52921 atggccatca aagcaccaga acaagctaga aatgaaaagt taggagaatt gcgttgatga
    52981 ttacaattag aaatttgagc aagacattct cagggcaaaa agttttagat agtttggctc
    53041 ttgatattga aaaaggtcaa gtgattgctt tagttggtgc ttcaggagca gggaaatcga
    53101 ccttccttcg tagcctaaac tatttggaaa aaccagattc aggatccatt agcattggtg
    53161 actttacggt tgattttgag accattacca cagagcaagt ccttatttta cgtcggaaac
    53221 ttgccatggt ttttcaacaa tttaatcttt ttgagcgtcg tacagccctt gagaatgtca
    53281 aagaaggtct taaggtggtc aaaaaactgt ctgaccaaga agcgactaag ctggcgcaag
    53341 cagaattggc taaggttggt ttagcagatc gaaaacatca ttacccacgt catttatcag
    53401 ggggacaaaa acaacgggta gctttagcaa gagctctggc tatgaaacca gatgttttat
    53461 tactagatga gccgacctcg gctcttgacc cggaattagt tggcgaagtg gaaaagtcta
    53521 ttactgatgc tgctaaatcc ggacagacca tggttttagt gagtcatgac atgaattttg
    53581 tttaccaagt ggcagataga gtactctttt tagatcaagg gaaaatcttg gaacaaggaa
    53641 cacctgaaga agtctttaga catcctcaaa aagaacgaac caaagaattt tttgcaagct
    53701 attcaaaaac atacatttaa tgatattctt ttgttataat aaaaaggtct aggtattcct
    53761 gggcttttct tgttatgttt tttagaaaaa cttatgggaa agaaataatg actaggtgga
    53821 aatatgttag tagaaatctt tagcctttat gtaaaaggtc tcattctctc aagccttact
    53881 atgattttaa tttgtttaat atggacttta tggcgagcaa aaaccaaaag agataaaaca
    53941 ttgattgaaa gacaagcttt tctttatgag atgctaatgg ttgctatttt aaccatccca
    54001 attttatcgt ttgcttttat gagtattttg gttgtcataa aatcctaatc attgcaagaa
    54061 gggattatca ttgctaaaat attggcaaga gtagattaaa ggagaaaaat catgtacgat
    54121 acattaatta ttggttcagg accagcagga atgactgccg ctttatacgc tgctagaagt
    54181 aatttatctg ttgccattat tgaacaaggg gcacctggtg gtcagatgaa caacactttt
    54241 gatatagaga attacccggg atatgatcat atatctggac cagaattagc tatgaaaatg
    54301 tacgaacctt tagagaagtt taacgttgaa aacatttatg gtattgttca aaaaatagaa
    54361 aattttggtg actataagtg tgtgttgact gaagatgcta gttatgaagc taaaacggtt
    54421 attattgcaa cgggagctaa ataccgtgta ctaggtgttc caggtgaaga atactatact
    54481 agtcgaggag tttcttattg tgcagtatgt gatggtgcgt ttttccgtga ccaggatctt
    54541 ttggtcgttg gtggtggtga ctcagcagtt gaagaagcta tttatcttac tcagtttgct
    54601 aaaaaggtaa ccgttgttca tcgccgtgat caactaagag ctcaaaaaat actgcaagat
    54661 cgtgcttttg ccaatgataa ggtcgacttt atctgggatt cagtcgtcaa agaaattcaa
    54721 ggcaatgata ttaaggtctc caatgtccta atcgaaaatg tgaaaacagg ccaagtcact
    54781 gaccacgctt ttggaggtgt gtttatctat gttggtatga atcctgtgac aggtatggta
    54841 aaggatctag aaattaccga tcaagaaggt tggattatca cagatgatca tatgaggaca
    54901 agtattcctg gtatttttgc gattggagat gttcgtcaaa aagaccttcg ccaaatcacg
    54961 acagctgttg gagatggtgc aattgcaggg caaggtgttt atcactacct tgaaagtttc
    55021 ccttcataag gttaaaactc cttcattttt ccttgtcttt cccttaaaaa agctaaattg
    55081 cataagacct ttaagaaata taaagaacag ttttccttaa tatctttcct agataaatgt
    55141 tgaaaatttt ctgaaaaaag gatataataa atagtatttt agtgaatgga gtttagaatg
    55201 tctataaaag aacaaacaga caataacgaa ctagaaaatg gaatggtaag gggcttagaa
    55261 aatcgtcatg tgcagttaat tgcgattgca ggaacgattg gaacaggtct ctttttaggg
    55321 gcaggacgct ctattgccct aacaggacca tctattattt ttgtgtatat gattacaggt
    55381 gccttcatgt ttatgatgat gcgtgccatc ggtgaaatgc tctattatga tccagatcaa
    55441 cacaccttta tcaattttat ttccaaatac attggtccag gctggggtta tttttcgggt
    55501 ctatcctatt ggatttccct tatttttatt ggaatggcag aaatcacagc agtaggtgct
    55561 tatgtgcaat tttggttccc aagctggcca gcctggttga ttcagttggt tttcttagtc
    55621 ttacttagtt cgatcaactt aattgctgtg cgcgtttttg gggaaacaga gttttggttt
    55681 gccatgatta agattttagc tattttagct ttgattgcaa cagctatttt catggtattg
    55741 acaggttttg aaactcacac aggccatgct agcctttcca atatttttga ccatttttcc
    55801 atgttcccaa atgggaaact aaagttcttt atggccttcc aaatggtttt ctttgcttat
    55861 caagctatcg aatttgtggg aattaccact tctgagacgg ctaacccaag aaaagttcta
    55921 ccaaaggcta ttcaagaaat tccaacccgt attgtgatct tttatgtggg agccttggtc
    55981 tctattatgg caattgtgcc atggcatcag ttaccagttg atgaatctcc ttttgtgatg
    56041 gtgttcaaat tgattggtat taaatgggca gcagccttga ttaactttgt ggtcttgaca
    56101 tcagcagcgt cagcgcttaa ctcaaccctt tattcaaccg gtcgtcatct ctatcagatt
    56161 gctaatgaga ctccaaacgc tttgaccaat cgcttaaaga ttaatacttt atctcgacaa
    56221 ggggtgccaa gtcgtgccat tattgcttca gcggttgtgg ttggtatctc agctttgatt
    56281 aatatcctac caggagttgc agatgcgttc tcactcatta cggcctcctc atcaggtgta
    56341 tatattgcta tctacgcctt aaccatgatt gctcactgga agtaccgtca atctaaggac
    56401 tttatggcag atggttattt gatgccaaaa tataaagtga caacgccttt gacccttgct
    56461 ttctttgctt ttgtctttat ctctcttttc ttacaagaat ccacctatat cggcgccatt
    56521 ggagcaacga tttggattat tgtttttggg atttatagca atgtcaaatt taaataataa
    56581 ttagcgatat agaactgaat ccattaactc tttcttattt gaggatgtca tgatggatca
    56641 gttctttttt tactttagcg ataactcaag ttatttctaa accctagcca aagagtcctt
    56701 tttaaaatat gttataatag taacaataat tcaaaggagt cccctttatg tataaagatg
    56761 atagtttaac tctccatact gatttgtatc agattaatat gatgcaagtt tattttgagc
    56821 agggcattca taatcgtcat gcggtgtttg aagtctattt ccgcaaggaa ccttttaaca
    56881 atggttatgc tgtttttgcc ggtttacagc gcatggtgga gtatctagag caatttcaat
    56941 tttcagaaac agatttggct taccttgaag aactgggtta tccaggaaac tttttgacct
    57001 accttaaaga gttacgatta gaattgacca tccgttcagc taaagaagga gatttggttt
    57061 ttgctaatga gccgattgtg caagtagaag gccctctagg tcagtgtcag ttggtcgaaa
    57121 cagctctttt aaacattgtt aacttccaga ccttgattgc tacaaaggct gctcgtattc
    57181 gttctgtgat tgaggatgaa ccccttttgg aatttggtac ccgtcgtgca caggaactag
    57241 atgctgcgat ttggggaacc cgcgcagcga tgattggtgg agcagatgca acaagtaatg
    57301 tgagagcagg taaacgcttt gatatccctg tttcaggtac gcatgctcat gctttagtgc
    57361 aagcctatgg taatgattat gatgctttca tggcttatgc taagacccat aaagattgtg
    57421 tatttcttgt tgacacctat gacacactta aagtaggtgt gccaacagct atccgagtag
    57481 ccaaagaaat gggtgacaag attaattttc taggtgttcg attggactct ggtgacttgg
    57541 cttacctctc aaaaacagtc cgtcagcagt tagacgacgc tggttttacc gaggctaaga
    57601 tctatgcttc taatgacctc gatgaaaata ccatcctcaa cttaaaaatg caaaaggcca
    57661 aaattgatgt ttggggtgtt gggaccaaac tgatcacagc ctatgatcag cctgccttag
    57721 gtgctgttta taagattgtc tctattgagc aagaagatgg tagcatgcgt gatacgatca
    57781 aattatcaaa taatgcagaa aaagtatcca caccaggtaa aaaacaagtt tggcgcatca
    57841 ccagtcgtga aaaaggcaaa tcagaaggcg actatatcac gtttactgat attaatgtta
    57901 atgaattaac tgagattgag atgttccatc cgacctatac ctatattaag aaaaccgtca
    57961 gaaattttga tgcaattcct ctcttagttg atatttttgt caagggagaa ttagtttacc
    58021 agctaccgac cttatctgaa atcaagactt atgctaaaaa agaatttgac aagctttggg
    58081 atgaatacaa gcgtgtctta aatcctcaag actacccagt agacttggca cgagatgtgt
    58141 ggcaaaataa gatgaccttg attgataaca ttcgtaaaga tactcatgga aagagtgaat
    58201 gacatgacat tacaagaaga gatcattcga caattaggcg tcaaggctag cattgatcca
    58261 caagaagaaa ttcgaaaaac cgttgatttt ttaaaagctt atttgcgaaa gcactcattt
    58321 ttaaaaactt atgtcttagg catttctgga ggtcaggatt ctaccctcgc aggaaagcta
    58381 gcccagatgg ccattgcaga gttacgggag gaaacaagtg ataaagctta ccaatttata
    58441 gctgttcgct tgccttacgg ggttcaggct gatgaagcag atgcgcaaaa agccttggct
    58501 tttattgcac cagatcagac ccttaccatt aatatcaagg cagctgtgga cggtcaagta
    58561 gaggctctac aagcagcagg cgttgagatt tctgatttta acaaaggtaa tattaaagca
    58621 cgtcagcgaa tgataagtca atatgccatt gctggccaaa tggctggtgc tgttattgga
    58681 acggatcatg cagcagaaaa catcactggt ttctttacta aatttggtga tggtggagca
    58741 gatattcttc ccctctttag gcttaacaaa cgtcaaggaa aggctctttt gaaagtttta
    58801 ggagcagatg ctgctttgta tgaaaaagtt ccaactgctg atttagaaga tcaaaaacca
    58861 ggcttggctg atgaggttgc cctaggggtg acctatcaag acatcgacga ctatttggaa
    58921 gggaagctga tttccaaagc agctcaagca actattgaaa aatggtggca taaaggacaa
    58981 cacaagcgtc acctgccaat caccattttt gatgattttt ggaaataaga aatgatgttc
    59041 aaagagtaaa aaaagaaaac aaaaaaagtt aggttatcct aacttttttc ttgaagaaaa
    59101 tcaaataagg tctataatag aagtaatcct ttagaataga atggctggat aataaccatc
    59161 ttgccaatat tatttcaggt gaagcttatc ttaatatttt aggaggacaa taatgtcagc
    59221 attaacagag acatttacag aacaattatt tgctcattac gaagccaatg caaaattttc
    59281 agctattgag aatgccgtta cgcataatgg tttgctaaaa tcattagaaa cccgtcaaag
    59341 tgaagtggac aatgattttg tgttttctat tgatttaact aaagataagg tatctaacca
    59401 aaaggcatct ggtcgctgct ggatgtttgc tgccttgaac actttccgac acaaattaat
    59461 cacagagttt aaattagaaa attttgaact gtcacaagcc catactttct tttgggataa
    59521 gtatgaaaaa gctaactggt ttatggagca ggtcattgct acagctgacc aagaattaac
    59581 cagtcgtaaa gttaagtttc tcttggatgt tccacaacaa gatggtggcc agtgggacat
    59641 ggtggtttcc ttgtttgaga agtatggagt ggtgccaaaa tctgtttacc cagaatcaat
    59701 ctcatcaagt aacagtcgtg aattaaacca gtatttgaat aaattattgc gccaagatgc
    59761 tcaaattctt cgtgacctga ttgcatcagg tgctaaggca gaccaagttg aggatcgaaa
    59821 agcagagctt cttcaggaaa tttttaactt cttggccatg acccttggtt taccacctcg
    59881 tcattttgat tttgcctacc gtgataaaga tgaccactat catgttgaga aaggtttgac
    59941 accgcaagcg ttttacgaca aatttgttgg cttaaagctt tctgattatg tgtctgttat
    60001 taatgcacca actgctgata agccttatgg caaatcatac actgttgaga tgctaggaaa
    60061 tgtggtcggt agtcgtgagg ttcgctacct caaccttgat atgaaacgtt tcaaagaact
    60121 agctatcaag cagatgcagg ctggtgagtc cgtttggttt ggctctgatg tgggtcaagt
    60181 ttcagatcgt caaaaaggga ttttggcaac taatacttac gattttgaag ctagtatgga
    60241 tatcaatctt agtcaagata aggctggacg tttggattat agtgaaagtt tgatgacaca
    60301 tgctatggtt ttaacaggtg ttgacttaga tgaaacagga aaaccgttaa aatggaaggt
    60361 tgaaaattca tggggtgaga aggttggtga caaaggttac tttgttgctt cagatgcttg
    60421 gatggatgaa tatacctatc aaattgttgt ccgtaaagaa ttcttaacag ctgacgagtt
    60481 agcggcttat gaaaaagagc cacaagtact agctccttgg gatccaatgg gtgctttagc
    60541 acaatgctaa ttcctttctt tgcgactgta tgaaatctaa aggctggaca aaacgaacag
    60601 caaacggatt ggactccgca taagaaagtg aacatgaaaa aaattattat gctaagaaaa
    60661 tgacatatga aaaaacacgt cttcggtata tggaaacgtg ttttttcatg tttactctct
    60721 tatggcatta gttgctattt ataacaattt taaataagtc tattttgttt taggttaacg
    60781 tttgaaaaat cagttgctaa ttcccttaac gttgtacctt ttctttaata cgattaaaga
    60841 attctgtttt ttgttttata ttgtatgtgg taagtcattt gtgtcatata aaaacgagtc
    60901 ctcacagaac gtggactcgc tttttattta gattatcact taaggtagat attaaaagta
    60961 actaaatgga tatgtaataa aaggtaggta cagacaataa gtagagttag agattaatga
    61021 gttgtctttt tatcatcggt agatggatga ttgctattgt tgtctgtatt ggttgaatta
    61081 cttatatcat tgctactact agtatcatct gaagtcgttt ggctagaagc tgttgtcgtg
    61141 ttattgctgt aaagattgtt gtaaaccgaa ttagtatagt cggtgttgct cgcataggtt
    61201 ccgcttaagt agaggaatcc accactgcga taaagaccat ttggcatcgt ccaatcttca
    61261 ctgtaaccat ttgttaagta agtcatcatg ctacgataaa cgtcagatgc aatctctaga
    61321 cttgatccgt atactggggt caagcggttt ttgtaacctg tccaaacagc catggcgtag
    61381 cgcttagtaa agccaacaaa gttttcgtct ggcgctaatg taccaacata atctggataa
    61441 aggccgtatt tttcaccaat tttagctagt tcctcatcag tgtagttaga agtccctgtt
    61501 ttaccagctt gcgcaacacc aggaatggca gcagcagtac ctgtaccata agtgagaaca
    61561 gtttttaaca tatctgtcat catataggcc gtggtttctt tcatggcacg ttttcctttt
    61621 tcatcaaaag ttttacttgt accatcacta aattccactt tattgacata ccttggttta
    61681 tgataaatac caccatttgc aaaagcagcg tatgcagcgg ccattttttc actacttgca
    61741 ccatattttt tatctgagct actgttatta cttgagatag cgtttgagta gtgcatttcg
    61801 ggatagttaa tacctaaact gcttaagaaa gatcgagcat agtcaagtcc tgcggcttcc
    61861 agtgctcgga ctgctgggac atttcgcgat agcataatag cagcttggat tgtcatccat
    61921 ccgttatatc taaggtccca gttaaacaat tgggtagtgg ttccaggcca ataatagact
    61981 gagtcattag tcgactgagc agtagaagta taaacaccag attcaatagc aggagcatag
    62041 gctgtgattg gcttcatggt agaaccccag tcacgatcag ttaaaacagc ttggttagtc
    62101 ccaaatgaaa cattttcatc ttgattacgt ccgccaagct gagcaataac atgaccattt
    62161 gtcacatcaa caatcgttga agcaacttgg aaatcttggt ctggatagta aacataatca
    62221 ccagaatgat aaatattata aagagtctgc tgcgcatcgg ggataatatt ggtataaact
    62281 tttaaaccag cggtaaaaat atctttattc gtttcttttt tgacttcttc aataacttgt
    62341 tttagataat tatccatata ttttggataa gttgagcgct gttggagtga ttgtagacct
    62401 tcagcgacgg gagttgcgat ggcagtttca tattctgctt tcgtcagatg tttttccatg
    62461 tacatctgtt gcaacacgac gttacggcga ttttgagcag cttcaggatg aaggtaagga
    62521 tcatattgac taggagcttg agggattcca gccaataggg ctagttgggc ataagataaa
    62581 tccttaagat ccttgccata ataagactta gcggctgtca gcataccata gttgccatta
    62641 cccatatata ctttgttgat gtaaaaagtc aggatttctt gtttagtata ttttcgctcc
    62701 atttgaagag caagccaaac ttcttgagcc ttacgtttta aggtttgatc agattcatta
    62761 gtagaaaaat aggctagttt aatgagttgc tgatcaagcg ttgacccccc ttgagtggtc
    62821 tgactcgtta gattatgaaa ggcagcacca aaaatacgat aaagatctac tccacgatgg
    62881 ttaaagaaac gtttatcttc aattgaggta atagcattaa ctagattaat agggatacta
    62941 tcagctgtta cattttcacg cttttcagaa cccaaatcag caatcagatt gttattaccg
    63001 tcataaacca agctagagtt tgttgatttt aactgggctt ctgacagttt cggagcactg
    63061 ctaatgtaga aggtaaacaa aagaccacca ataataataa caaggataat aaggctaaga
    63121 attgcactta atacatactt tagccactta aggatttttg gatttttaat tgtaatcacc
    63181 gcctaaaaaa ttttgttcaa ttatgtttaa ataagggact tgtggaaagg ctccaaatgc
    63241 taccttaaag ccattttttc tgatataatc aatgggcatc gatttactgc cattatctat
    63301 ctgataaaat gaaatcaagg cttgagcagg taggtagtaa gtctccttca gtgtggaaaa
    63361 gtgaagaagg acaaagcaga ttcctttttg atgtaaaacg caagccatat gttcaatctg
    63421 atgtaaatgg aaatttttca tgggcatagc tgttttttgc ctggtttctt ttgcttcaaa
    63481 atcaacataa tgtcctttat aaacgccgca atagtcagtc gttgaagctt gtctaaagta
    63541 cgcttcaacg atttttgccc gactgcgttt agggtaatcc actttgacaa tttggacagg
    63601 agtaggtttt ttgtgaatga cagctatttg acgagataaa taataatcat ttgtcgcgtt
    63661 gatagcagct tcaaaggaca tacctctatt ggcgaagtcc accttatttt gctttgtttg
    63721 cttttgaacg gaacttactt tttgtcgaat aagattatga ggataattaa ccattgttct
    63781 cctaatatta tctagttaat gctaaaaatt taagcacttt tattataaca tattttttga
    63841 aaggataaca cctaatgact gcgattctaa ttacaggtta ccgaagcttt gaaataggga
    63901 tttttgacca taaagaccca cgagtttcaa ttattaaaca ggctattcgt aaagatttaa
    63961 taggttattt ggaaaatggt gtggattggt ttatctttac agggaacttg ggttttgaac
    64021 aatgggcttt ggaagtagct aatgagctaa aagaggagta tcccttgcag atagctacta
    64081 tattcttgtt tgaaacgcat ggagataggt ggaatgaaaa aaatcaaaaa gttttaagtc
    64141 agtttagggc tgttgacttt gtaaaatatt actttccaaa ttacgaacaa ccgacgcaat
    64201 ttagtcaata ttatcagttt ttattagaaa agacagaagg ggcttatgtt ttttatgaca
    64261 cagaaaacga gaccaactta aaatattttc tcaagaaagc aaaagatatg ccgcattatc
    64321 agttgctatt gctaaccttt gatcggctaa atgatatgtc acaaagctag cattgtattt
    64381 ttaggggtat tggtatttaa atacttgctt tttgaactat ttttctgtat cattaataat
    64441 aatactgaaa tataattttg gtaaaaagat tgaccgttcg gagagta
//
LOCUS       DAUDYX010000004        62058 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.004, whole genome shotgun sequence.
ACCESSION   DAUDYX010000004 DAUDYX010000000
VERSION     DAUDYX010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 62058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62058
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.004"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            69..860
                     /locus_tag="VHD02_000226"
     CDS             69..860
                     /locus_tag="VHD02_000226"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEQ5496865.1"
                     /translation="MQFKHIIFDVVDDLAILTLNRPEVSNGFNIPICQEILVALAEVK
                     RDTSVRFLLIKAVGKVFSVGGDLVEMQEAVAKDNVQSLVKIAELVQEISFAIKHLPKP
                     VILCADGAVAGAAFNIALAVDFCIASTQTKFIQAFVNVGLAPDAGGLFLLTRAVGLNR
                     ATHLVMTGEGITADKGVDYGFVYRTAESDKLDKVCLQLLKRLRRGSSNSYAGMKSLVW
                     QSFFTGWEDYAKAELAIQEELAFKEDFKEGVIAFGERRRPNFQGK"
     gene            934..1368
                     /locus_tag="VHD02_000227"
     CDS             934..1368
                     /locus_tag="VHD02_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEQ5496866.1"
                     /translation="MEYDKINHYLVDIFNRILVIEEMSLKTSQFNDVSLKEMHTIEII
                     GKYDQVTPSDIARELMVTLGTVTTSLNKLEAKGYIARTRSRSDRRVVYLSLTKRGRLL
                     DRLHAKFHKNMVGHVIADMSDEEMQALVRGLGNLHQFLEDLV"
     gene            1369..2343
                     /locus_tag="VHD02_000228"
     CDS             1369..2343
                     /locus_tag="VHD02_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEQ5496867.1"
                     /translation="MIFSKISQVAHYVPQQLVTNNDLASIMDTSHEWIFSRTGIAERH
                     ISRDEMTSDLAIQVADQLLTQSGLKADAIDFIIVATISPDATMPSTAAKVQAAIAATS
                     AFAFDMTAACSGFVFALAMADKLIASGAYQNGMVIGAETLSKLVNWQDRATAVLFGDG
                     AGGVLLEASKDKHVLAETLHTDGARCQSLISGETSLSSPYSIGKKAIATIQMDGRAIF
                     DFAIRDVSKSILTLMAQSDITKDDIDYCLLHQANRRILDKIARKIDVPREKFLENMMR
                     YGNTSAASIPILLSEAVQKGQIRLDGTQKILLSGFGGGLTWGSLIVKI"
     gene            2404..2628
                     /locus_tag="VHD02_000229"
     CDS             2404..2628
                     /locus_tag="VHD02_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEQ5496868.1"
                     /translation="MAVFEKVQEIIVEELGKETEEVTLETTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKSK"
     gene            2830..3801
                     /gene="fabK"
                     /locus_tag="VHD02_000230"
     CDS             2830..3801
                     /gene="fabK"
                     /locus_tag="VHD02_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEQ5496869.1"
                     /translation="MKTRITELLNIDYPIFQGGMAWVADGDLAGAVSNAGGLGIIGGG
                     NAPKEVVKANIDRVKAITDRPFGVNIMLLSPFADDIVDLVIEEGVKVVTTGAGNPGKY
                     MERLHQAGIIVVPVVPSVALAKRMEKLGVDAVIAEGMEAGGHIGKLTTMSLVRQVVEA
                     VSIPVIAAGGIADGHGAAAAFMLGAEAVQIGTRFVVAKESNAHQNFKDKILAAKDIDT
                     VISAQVVGHPVRSIKNKLTSAYAKAEKAFLIGQKTATDIEEMGAGSLRHAVIEGDVVN
                     GSVMAGQIAGLVRKEESCETILKDIYYGAARVIQNEAKRWQSVSIEK"
     gene            3826..4764
                     /gene="fabD"
                     /locus_tag="VHD02_000231"
     CDS             3826..4764
                     /gene="fabD"
                     /locus_tag="VHD02_000231"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEQ5496870.1"
                     /translation="MTKTAFLFAGQGAQKLGMARDFYDNFAIVRKTFDQASQVLGYDL
                     RRLIDSDELKLNQTSYTQPAILTSSIAIYRVLGLHHVKPDMVAGLSLGEYSALVASGA
                     LSFEDTLSLVAKRGRLMEEAAPQGSGKMVAVMNTDVQVIEEVCQIAAKHEVVAPANYN
                     TPSQIVIGGQTDAVNVAVELLKERGVKRLIPLNVSGPFHTALLEPASRLLAKELERYN
                     FSDFKIPLVGNTEANIMEKDRIPELLARQVMEPVRFYDSVATLVESGITQFIEVGPGK
                     VLTGFVKKIDKNLLCTSVENMVSLRLFLDREDIHGN"
     gene            4754..5488
                     /gene="fabG"
                     /locus_tag="VHD02_000232"
     CDS             4754..5488
                     /gene="fabG"
                     /locus_tag="VHD02_000232"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEQ5496871.1"
                     /translation="MEIKGKNIFITGSTRGIGLAMAHQFASLGANIVLNGRSAISEEL
                     VASFTDYGVTVVTISGDVSEASEAKRMVNEAIESLGSIDVLVNNAGITNDKLMLKMTE
                     EDFERVLKINLTGAFNMTQSVLKPMIKARQGAIINVSSVVGLTGNIGQANYAASKAGM
                     IGFTKSVAREVAARNICVNAIAPGFIESDMTGVLPEKMQEQILSQIPIKRIGKAQEVA
                     HLASFLVEQDYITGQVIAIDGGMTMQ"
     gene            5503..6735
                     /gene="fabF"
                     /locus_tag="VHD02_000233"
     CDS             5503..6735
                     /gene="fabF"
                     /locus_tag="VHD02_000233"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEQ5496872.1"
                     /translation="MTFKRVVVTGYGLTSPIGHDPETFWDNLKAGQIGIGPITKFDTT
                     DYAVKNAAEIQDFPFDKYFVKKDLNRFDRYSLYALYAAKEAINHADLNIEMIDSDRFG
                     VIVASGIGGIAEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMSLKAQGVCKSI
                     NTACASSNDAIGDAFRAIKFGTQDVMIVGGSEAAITKFAIAGFQSLTALSTTEDPSRS
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAQERGATILAEIVGYGNTCDAYHMTSPNPEG
                     LGARKAIHLALQEAGIEASAINYVNAHGTSTPANEKGESQAIVAVLGKDVPVSSTKSF
                     TGHLLGAAGAIEAIATIEAMRHNYVPMTAGTQALSEDIEANVIFGEGKETAINYAISN
                     TFGFGGHNAVLAFKCWEE"
     gene            6737..7237
                     /locus_tag="VHD02_000234"
     CDS             6737..7237
                     /locus_tag="VHD02_000234"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEQ5496873.1"
                     /translation="MNIQEIKDLMAQFDTSSLREFLFKTNEGELIFSKNEQHLNASTS
                     NQEHAVPVPQVQLVPNPTASEASSPASVKDVPVEEQPQAESFVAEGDIVESPLVGVAY
                     LAASPDKPPFVAVGDTVKKGQTLVIIEAMKVMNEVPAPCDGVITEILVSNEDVIEFGQ
                     GLVRIK"
     gene            7234..7656
                     /gene="fabZ"
                     /locus_tag="VHD02_000235"
     CDS             7234..7656
                     /gene="fabZ"
                     /locus_tag="VHD02_000235"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEQ5496874.1"
                     /translation="MMDIREIQAALPHRYPMLLVDRVLEVSDDHIVAIKNVTINEPFF
                     NGHFPHYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFIKRRGTIAVVEARAEVDGKLAASGTLTFACGQ"
     gene            7688..9052
                     /locus_tag="VHD02_000236"
     CDS             7688..9052
                     /locus_tag="VHD02_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEQ5496875.1"
                     /translation="MFKKILIANRGEIAVRIIRAARELGISTVAVYSEADKEALHTIL
                     ADEAICIGPARSKESYLNMNSVLSAAIVTGAQAIHPGFGFLSENSKFATMCEEMNIKF
                     IGPSASVMDKMGDKINARSEMIKAGVPVIPGSDGEVYNAQEALAIANKIGYPVMLKAS
                     AGGGGKGIRKVETEADLEAAFNAASQEALGAFGNGAMYLEKVIYPARHIEVQILGDAY
                     GNIIHLGERDCSLQRHNQKVLEESPSIAIGNTLRHEMGQAAVRAAKAVAYENAGTIEF
                     LLDEDSEKFYFMEMNTRIQVEHPVTEFVTGVDIVKEQIKIAAGQPLAINQEDITITGH
                     AIECRINAENTAFNFAPSPGKITDLYMPSGGVGLRVDSAVYNGYAIPPYYDSMIAKII
                     VHGSNRFDALMKMQRALVELEIEGIITNTDFQLDLISDKRVIAGDYDTSFLMETFLPH
                     YNKD"
     gene            9061..9927
                     /locus_tag="VHD02_000237"
     CDS             9061..9927
                     /locus_tag="VHD02_000237"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEQ5496876.1"
                     /translation="MALFRKKDKYIRITPNNSLKGSVSHNVPEVPDELFAKCPACKHM
                     IYKKDLGLAKICPTCSYNFRISAQERLTLTVDEGSFQELFTSIETKDPLRFPGYQEKL
                     QKAKETTGLHEAVLTGKAMVKEQKIALAIMDSHFIMASMGTVVGEKITRLFELAIEEN
                     LPVVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNAGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQSLVGFAGRRVIETTVRENLPDDFQKAEFLQDHGFVDAIVKRTELRDK
                     IAHLVAFHGGGQ"
     gene            9924..10694
                     /locus_tag="VHD02_000238"
     CDS             9924..10694
                     /locus_tag="VHD02_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEQ5496877.1"
                     /translation="MTDVSRVLKEARDQGRLTTLDYANLIFDDFMELHGDRHFSDDGA
                     IVGGLAYLAGQPVTVIGIQKGKNLQDNLARNFGQPNPEGYRKALRLMKQAEKFGRPVV
                     TFINTAGAYPGVGAEERGQGEAIAKNLMEMSDLKVPIIAIIIGEGGSGGALALAVADQ
                     VWMLENTMYAVLSPEGFASILWKDGSRATEAAELMKITAGELYKMGIVDRIIPEHGYF
                     SSEIVDIIKANLIEQITSLQAKPLDQLLDERYQRFRKY"
     gene            complement(10916..12193)
                     /gene="serS"
                     /locus_tag="VHD02_000239"
     CDS             complement(10916..12193)
                     /gene="serS"
                     /locus_tag="VHD02_000239"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEQ5496878.1"
                     /translation="MLDLKRIRTDFDTVAAKLKNRGVSEDTLTHLKELDEKRRTLLVQ
                     SEELKAERNIASAAIAQAKRQKEDATQQIADMQKVSADIKTIDNQLVAIDQQVTDIIT
                     VLPNTPHDSVPVGADEEDNVEIRRWGTPRDFNFEVKAHWDLGEDLDILDWERGAKVTG
                     ARFLFYKNLGARLERALYNFMLDEHIKEGYQEIITPYMVNHDSMFGTGQYPKFKEDTF
                     ELADTNFVLIPTAEVPLTNYYRGEILDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLGLPYRVISLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGGETVISPK"
     gene            complement(12584..12943)
                     /locus_tag="VHD02_000240"
     CDS             complement(12584..12943)
                     /locus_tag="VHD02_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEQ5496879.1"
                     /translation="MAQSLNTSVEYKTKAVSYLGMGGKVGHILLGDKALEFYNDKNVN
                     DYIQIPWTAINHIGANVSRKKVSRHFEIFTDQGKFLFASGDSGKILKITRQHIGNEKV
                     ITLPTLMQTFINKFFRH"
     gene            complement(13057..13968)
                     /locus_tag="VHD02_000241"
     CDS             complement(13057..13968)
                     /locus_tag="VHD02_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEQ5496880.1"
                     /translation="MTEQIKLTKSDRQRVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTSPEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEERANGTPIDDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASTGNIVGPLLFFFGWNLIRMAFLWYTQE
                     FGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVSINFTIDLPGKQLSDGA
                     YVVFPDGAVKGAELKTILANAIGGMSLDKVQAQTLQGQLDSLIPGLAGLLLTFLCMWL
                     LKKKVSPIAIIIGLFAFGILAHLAGIM"
     gene            complement(13985..14794)
                     /locus_tag="VHD02_000242"
     CDS             complement(13985..14794)
                     /locus_tag="VHD02_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEQ5496881.1"
                     /translation="MSDISIISAILVVIIAFFAGLEGILDQFQMHQPLVACTLIGLVT
                     GHLEAGVILGGTLQMLALGWANIGAAVAPDAALASVAAAIIMVKSGDFTQKGITFAYS
                     TAIPLAVAGLFLTMIVRTLSTALVHAGDKAAAEGNFAGIERFHFIALLLQGLRIAVPA
                     ALLVAVPTSAVQSVLNAMPNWLNEGMQIGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAAISQLTLIAMGVIGVAIAFIYLNLSKKGGNGGNAAGSADPIGDILEDY"
     gene            complement(14883..15875)
                     /gene="manX"
                     /locus_tag="VHD02_000243"
     CDS             complement(14883..15875)
                     /gene="manX"
                     /locus_tag="VHD02_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEQ5496882.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIQQFDADDEILVLADLWSGSPFNQASRVAGENPERKMAIITGLNLPMLIQ
                     AYTERLMDAGAGVEQVAANIIKESKDGIKALPEDLNPVEETAATEKVVNALQGAIPAG
                     TVIGDGKLKINLARVDTRLLHGQVATAWTPASKADRIIVASDEVAQDDLRKQLIKQAA
                     PGGVKANVVPISKLIEASKDPRFGNTHALILFQTPQDALRAVEGGVEINELNVGSMAH
                     STGKTMVNNVLSMDKEDVATFEKLRDLGVTFDVRKVPNDSKKNLFELIQKANIK"
     gene            complement(16227..17039)
                     /locus_tag="VHD02_000244"
     CDS             complement(16227..17039)
                     /locus_tag="VHD02_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ5496883.1"
                     /translation="MTKKLIAIDLDGTLLHHDNTISTYTQKTIKAVQDKGHHVIISTG
                     RPYRMALDYYLQLNLKTPIITFNGALTHMPEQKWAYEHNVTLDKGYLLRLLKYQDDFQ
                     MDFIASEYRKNVYITMTNPESIDPQLFGVDEITQDMALEITKITRNPNALLMQTHHED
                     KYALAKNMRAFFKDEIEIDSWGGPLNILEISSKNVNKAYALNYLLGIYNMDKKDLIAF
                     GDEHNDTEMLAFAGTSYAMKNASPVLLPYADQQLNFSNEEDGVAKKLEELFL"
     gene            17302..18762
                     /locus_tag="VHD02_000245"
     CDS             17302..18762
                     /locus_tag="VHD02_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEQ5496884.1"
                     /translation="MEKFFKLSENGTTVSTEIMAGLTTFFAMSYILFVNPSILGAAGM
                     PSNAVFLATIIAAAISTLIMGLFANVPYALAPGMGLNAFFTYTVVFALGFSWQEALAM
                     VFICGLFNIFITVTKFRKSIIKAIPVSLQHAIGGGIGVFVAYLGFKNANIITFSISAE
                     NIVMVNGVEPAKASAKTFADGLLFVDANGGVVPTISSFTDSSVLLAIFGLLLTTALVI
                     RNFRGAILIGIVATTLVGIPLGIVDVSNLNFGISHIGEAWTELGTTFLAAFDGLSSLF
                     SDSSRLPLVFMTIFAFSLSDTFDTIGTFIGTGRRTGIFSQDDENALENSIGFSSKMDR
                     ALFADAIGTSIGALVGTSNTTTYVESAAGIAEGGRTGLTAVSTAVCFLLSILLLPLVG
                     IVPAAATAPALIVVGVMMVSSFLDVNWSKFADALPAFFAAFFMALCYSISYGIAAAFI
                     FYCLVKVVEGKTKDIHPIIWGATFLFIVNFIILTIL"
     gene            18916..19377
                     /gene="tsaE"
                     /locus_tag="VHD02_000246"
     CDS             18916..19377
                     /gene="tsaE"
                     /locus_tag="VHD02_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEQ5496885.1"
                     /translation="MFYSENEYTLKAYGETLGTYLSIGDVIVLSGDLGAGKTTLAKGI
                     AKGMGISQMIKSPTYTIVREYEGRLPLYHLDIYRVGDDPDSIDLDDFLFGNGVTVIEW
                     GELLGEGLLQDYLQITITKRDKGRQLDLLAHGERSRQLLEIMSHDSAKSRY"
     gene            19352..19876
                     /locus_tag="VHD02_000247"
     CDS             19352..19876
                     /locus_tag="VHD02_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ5496886.1"
                     /translation="MIQQSLVIEEGVPQDAKAVLSLVTAAAKETDFITGVETILEATP
                     QELSDFLSRSQTSFIDFCLLARLDEKVVGLLNLSGEVLSQGQVEADVFMLVAKTYRGY
                     GIGQLLLEIALDWAEENPYIESLKLDVQVRNIKAIYLYKKYGFRIESMRKNDIKSKNG
                     DDLDIYHMRKSVSQ"
     gene            19885..21165
                     /locus_tag="VHD02_000248"
     CDS             19885..21165
                     /locus_tag="VHD02_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEQ5496887.1"
                     /translation="MKIGKKIVLMFTAIVLTTVLALGVYLTSAYTFSTGELSKTFKDF
                     STSSKKSDAIKQTRAFSILLMGVDTGSSERASKWEGNSDSMILVTVNPKTKKTTMTSL
                     ERDTLTTLSGPKNNEMNGVEAKLNAAYAAGGAQMAIMTVQDLLNITIDNYVQINMQGL
                     IDLVNAVGGITVTNEFDFPISIAENEPEYQATVAPGTHKINGEQALVYARMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALDSISSYRKILSAVSSNMQTNIEISSRTIPSLLGYRD
                     ALRTIKTYQLKGEDATLSDGGSYQIVTSNHLLEIQNRIRTELGLHKVSQLKTNATVYE
                     NLYGSTKSQTVNNNYDSSGQAPSYSDSHSSYANYSSGVDTGSSYQSASTDQDSTASSH
                     TPATPSSSSDALAADESSSSGSGSLVPPAINPQT"
     gene            complement(21276..21632)
                     /locus_tag="VHD02_000249"
     CDS             complement(21276..21632)
                     /locus_tag="VHD02_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEQ5496888.1"
                     /translation="MKVKTVIAVGLLSFTAYKAYQKRCTIKELLSISRQAKDAAQLDL
                     DNIKANLDLIHSQGKVIQNISQDLAHKWRYFNQETQAHLTEIQNRMAKYQEDSELISS
                     ESPTCQTVILTNNHSR"
     gene            complement(21629..22048)
                     /locus_tag="VHD02_000250"
     CDS             complement(21629..22048)
                     /locus_tag="VHD02_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEQ5496889.1"
                     /translation="MENCIFCSIIQGDIPSSKVYEDEQVLAFLDISQTTKGHTLVIPK
                     QHVRNLLEMTAETASHLFARIPKITRAIQSATGATAMNIINNNEALAGQTVFHAHVHL
                     VPRYNEEDGISIQYTTHEPDFPVLEKLARQINQEVSS"
     gene            22120..22845
                     /locus_tag="VHD02_000251"
     CDS             22120..22845
                     /locus_tag="VHD02_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5496890.1"
                     /translation="MLNIKNLTGGYHNIPVLNDVSFSVDNGELVGLIGLNGAGKSTTI
                     NEIIGFLKPYQGSISIDGLTLAENAVAYRQKIGFIPETPSLYEELTLSEHINTVAMAY
                     DIDLEVAQKRAQPFLEMFRLTDKLEWFPVNFSKGMKQKVMIICAFVIDPSLFILDEPF
                     LGLDPLAISDLIQTLEVEKAKGKSILMSTHVLDSAERMCDRFVILHHGQVRAQGTLAD
                     LQEAFGDRSASLNDIYLALTKED"
     gene            22848..23882
                     /locus_tag="VHD02_000252"
     CDS             22848..23882
                     /locus_tag="VHD02_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ5496891.1"
                     /translation="MKALFLKRRQNFQKQQNKYLRYVLNDHFVLVLMFLLGFAMVQYG
                     QLLNHFPTNHLPIQVCLGILIPLLLSMGSIATYLEEADQHFLLPKEEEVISYIKQAER
                     LSFLLWGTLQTAVLLFLYPIFRRLGLSLFIFIILVLILLALKRVVLSRKTRYFLRGNR
                     LDWAKAVAFESNRKQSILKFYSLFTTVKGISTKVKERTYLNPLLKLVKQTPSNLWLSL
                     YARAFLRSSDYLGLFLRLMLLSSLSVFFIHNLYLSVSLALIFNYLVVFQLLSLYYHYD
                     YHYMTSLYPENSRSKKKNVLSFLRGLSFLMLIVNMLCCSSAPKALILIVGMVFIACIY
                     LPYKLKKIID"
     gene            23946..24737
                     /locus_tag="VHD02_000253"
     CDS             23946..24737
                     /locus_tag="VHD02_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEQ5496892.1"
                     /translation="MTTTEQELTLTPLRGKSGKAYKGTYPNGECVFIKLNTTPILPAL
                     AKEQIAPQLLWAKRMGNGDMMSAQEWLNGRTLTKEDMNSKQIIHILLRLHKSKKLVNQ
                     LLQLNYKIENPYDLLVDFEQNAPLQIQQNSYLQAIVKELKRSLPEFKSEVATIVHGDI
                     KHSNWVITTSGMIFLVDWDSVRLTDRMYDVAYLLSHYIPRSRWSEWLSYYGYKNNDKV
                     MQKIIWYGQFSHLTQILKCFDKRDMEHVNQEIYALRKFREIFRKK"
     gene            24737..25372
                     /gene="trmB"
                     /locus_tag="VHD02_000254"
     CDS             24737..25372
                     /gene="trmB"
                     /locus_tag="VHD02_000254"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEQ5496893.1"
                     /translation="MRVRKRKGAEEHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIE
                     VGSGKGGFITGMALKNPDINYIGIDIQLSVLSYALDKVLASEVPNVKLLRVDGSSLTN
                     YFEDGEVDMMYLNFSDPWPKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGLFE
                     YSLASFSQYGMTLRQIWLDLHASNYEGNVMTEYEEKFSNKGQVIYRVEANF"
     gene            25437..25523
                     /locus_tag="VHD02_000255"
     tRNA            25437..25523
                     /locus_tag="VHD02_000255"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25471..25473,aa:Ser,seq:gga)
     gene            complement(25570..26649)
                     /locus_tag="VHD02_000256"
     CDS             complement(25570..26649)
                     /locus_tag="VHD02_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEQ5496894.1"
                     /translation="MAFYRKLGSGWEYRITYRDSQGKKREKSKRGFKTKTLAKVAAQQ
                     AEIDLNTLTADLLDITVLDYNRRWADIYKRPHITAKTWQTYTKNFKHIEHYFGTRKLK
                     SITHTFYQQVLNDFGAKVAQQTLDKFHYQIKGACKMAIRDGIIRDNFADGAIVRSQKP
                     AKEESEKFMEESEYLAFIKVAKSKVKYPSYLTTYIIAVTGLRFAEVQGLTWKDIDFEN
                     GYIDINKTFDYSISQNFGPTKNEQSIRKVPIDKNSLEILRNFKSDYYQDNKLGRVCFG
                     ASNNATNKVIKRVTGRNLTNHSLRHTYASYLIAQGVDLISVSKLLGHENLNITLKVYA
                     HQIESLKEKNDHQVKNIFQNLKFDG"
     gene            complement(26766..27317)
                     /locus_tag="VHD02_000257"
     CDS             complement(26766..27317)
                     /locus_tag="VHD02_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015986700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496895.1"
                     /translation="MDIAKLKEFAITAAGKAKDGAIKANELRKKASQESKIILPPALN
                     LELGAQTAIRKTIDGKYYIGFYSETPELYEFVSLQFDGSRIIEKTVTKGKTTQKGRSS
                     SVLGGAAIGKVLAPGVGTIIGGMAGGARKKKGTINTTATTTSEEIPGKATVALRNIQT
                     GEVKTLKTKLTQAQYVSVNNFFK"
     gene            complement(27328..27711)
                     /locus_tag="VHD02_000258"
     CDS             complement(27328..27711)
                     /locus_tag="VHD02_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEQ5496896.1"
                     /translation="MMTPETVCQEKGIDLVYFDGRGTNTPGMFNKKHNVIAIDTYLDG
                     IYKHKVIYHELGHREHTASYYKLNKEKAELQADRCMIHHLLKEELSYWDNMEDFNYIQ
                     FMEKYELTSIADEVMIKEELEFLIS"
     gene            complement(27725..28126)
                     /locus_tag="VHD02_000259"
     CDS             complement(27725..28126)
                     /locus_tag="VHD02_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ5496897.1"
                     /translation="MLFYRYFLSSHRKADKSMFSTFERIKELAKSRGISLGLLEEKLG
                     LSRNSIYTIKNKKPSAERLQLIADYFHVSTDYLLGRTDNPKIAQDGHASVAIDLKKDA
                     EETFFFDGHELNDEDIDLITSILETRIKNRK"
     gene            28401..28556
                     /locus_tag="VHD02_000260"
     CDS             28401..28556
                     /locus_tag="VHD02_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496898.1"
                     /translation="MDEKNPLEQFEEFCKNNSFEYVKGWVDGMQSVLNILSMELSKKN
                     GSTSEVD"
     gene            complement(28537..28950)
                     /locus_tag="VHD02_000261"
     CDS             complement(28537..28950)
                     /locus_tag="VHD02_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496899.1"
                     /translation="MEKFNFWLTIATAVLPAAISAIISFLASRYKNRSDIESLEKKFN
                     HEMTTIEKNHSNDLERLEKQYQHDFEAMEKNHFRELEKMKEAHTLELENIQKQSEAEV
                     NNTVAQGLMTMLGSAMTPALSKYLEENDVFNQPPK"
     gene            28998..29213
                     /locus_tag="VHD02_000262"
     CDS             28998..29213
                     /locus_tag="VHD02_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ5496900.1"
                     /translation="MSQQHKKWSALVEQRLKEKGWTRVDLALAAGLRSSGTITDLLTK
                     GKGSVDLKVRVSKILSIREPWELFENE"
     gene            29275..29550
                     /locus_tag="VHD02_000263"
     CDS             29275..29550
                     /locus_tag="VHD02_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015986704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEQ5496901.1"
                     /translation="MDSRLLQMLDEFEAGLIDRKIKVMKMINNETEIYPLELNKKQVS
                     KMFGVDPKTFDARFNSHKDFPRIETGGREKYPRDLAIEWYHRNWSIT"
     gene            29547..29717
                     /locus_tag="VHD02_000264"
     CDS             29547..29717
                     /locus_tag="VHD02_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015986705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496902.1"
                     /translation="MNKLELFLLATTVILAVIARVQHEVIKKHNSPENKRRIFREVAL
                     ENSKKWSAERYV"
     gene            29710..29913
                     /locus_tag="VHD02_000265"
     CDS             29710..29913
                     /locus_tag="VHD02_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496903.1"
                     /translation="MSKWELQKQLLANATDFESTVEALFELNELGEISDEELLLNLRS
                     SYRKYSEEKLCIIDKLIKLGGAE"
     gene            29910..30296
                     /locus_tag="VHD02_000266"
     CDS             29910..30296
                     /locus_tag="VHD02_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496904.1"
                     /translation="MMTTFEKIIKNLNRIERELVKPEAERRDSLPILSAQLSAIKQDL
                     KLMLWIELPALNNVEKYEALYQGNDFKFSETFLERQATRKAFWRRLAKETFKEDQDKR
                     NFVKLAEKEFKGATLSWQEFLWGKKK"
     gene            30293..30445
                     /locus_tag="VHD02_000267"
     CDS             30293..30445
                     /locus_tag="VHD02_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496905.1"
                     /translation="MNISLKFIDETLAGLNDILRQGGLSCSQSQALADAVFILTALKQ
                     VIEERK"
     gene            30442..30645
                     /locus_tag="VHD02_000268"
     CDS             30442..30645
                     /locus_tag="VHD02_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496906.1"
                     /translation="MNYLEYALAYLERELEIIDDEVIEVELPGGDWEFVPNPYYEEGL
                     HDSPHYRSQVAKDILDIKGLLGR"
     gene            30733..31032
                     /locus_tag="VHD02_000269"
     CDS             30733..31032
                     /locus_tag="VHD02_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496907.1"
                     /translation="MIEVTFKAESMAGVFDAMREALDMPKTVTKDVVEEKPAPKKKEE
                     SVTLTLADIKKMAKAKVEEGKSKDIKEVLKEMNVAKVGELEEYQFAEFVEKLEAL"
     gene            31032..32186
                     /locus_tag="VHD02_000270"
     CDS             31032..32186
                     /locus_tag="VHD02_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015986706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HEQ5496908.1"
                     /translation="MPVENHALLSASSAHRWLYCPMLPRLEADYPSRDTVYTQEGTSA
                     HELSEIKLMYKSGKITKRKFNTLTKAFKENSDFYNEEMEEMTELYTDIVMEHLNAYED
                     AEIELEKRIDFSDWVPGGFGTSDVVILADGVIEIIDLKYGKGMPVSANQNPQMGLYAL
                     GAYASYDMVYDFDRIKMTIIQPRLDSVSSVDIYVEELLYWADNVVLPMAAQADAGIGD
                     WNLSEKVLQWSPVAAKLVPRAQENWELIDKYDYQEPVYLSDEAVAEILDKASAIKKWV
                     ESVEAYALKEALSGKEVPGYKIVEGRSNRVLTNKEAAATILEKNGFEDIYKPKELLSM
                     GALEKMVGKKRFEDMMAFILDKPQGKPVLVKNSDKRPALNSLEQAIKEFE"
     gene            32190..32588
                     /locus_tag="VHD02_000271"
     CDS             32190..32588
                     /locus_tag="VHD02_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496909.1"
                     /translation="MKIYKVKVKFRQTCHKKFKGKKYSYFSFEELRVGDLVVVETVYG
                     PSVAKVTEVVDANELFTATSYVISKVDTSLLAGKKELMATALTVKANIDAETAEFAAK
                     YKDAYYLGLFDQYKNQNPELAELLTQLKEL"
     gene            32599..33156
                     /locus_tag="VHD02_000272"
     CDS             32599..33156
                     /locus_tag="VHD02_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HEQ5496910.1"
                     /translation="MTPSTTKVVTGKVRLSYAALLEPKAFEGQEAKYSTVILIPKTDK
                     VTIKKIKDAQKAAYEAAKDNKLKGVKWERVKTTLRDGDEEMDTEEHPEYAGHMFMSVS
                     SKTRPQIIDRYKNPVDSADEVYSGVYARVSLNAYAYNTAGNKGISCGLNNVQIVAKGD
                     YLGGRSSADADFDEWNEEEDEDDIL"
     gene            33199..35121
                     /locus_tag="VHD02_000273"
     CDS             33199..35121
                     /locus_tag="VHD02_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEQ5496911.1"
                     /translation="MRHLNIDIETYSSNDIKNGVYKYADAEDFEILLFAYSIDGGEVE
                     CLDLTRQSLPEDIKDMLFDDKVRKHAFNAQFERVCLSRYLGLPYYLDPAQWQCTMVLA
                     QELGLPASLEKCALYLKLAQEKDAAGKNLIKYFSVPCKPTKANGGRTRNLPEHDPGKW
                     QMFINYCMQDVVVEMAIAEKLESVPVHDREWDYYVCDQRINDRGVALDKELVASALYC
                     KDVKMESLSGELKALTGLDNPNSRAQLLPWLKEHGYSANGLTKADVEQELKKAEGELK
                     RVLELKLQTAMSSLKKYEAMERAMCSDGRVHGLLQFYGASRTGRWAGRVVQVQNLARN
                     YIKDLDDAREYVKKRDIDAVEILYDSLNDTLKQLVRTALVAKDGYTFYVSDFSAIEAR
                     VIAWFAGEQWRLDVFSTHGKIYEASASQMFGIPIEEIDKELRQKGKISELALGYQGGP
                     GALKQMGALNMGVKEEELQGLVDDWRRANKKIVQFWKDVQRAAIKAIKSRAPIKLGKI
                     RFRYRKGFLFITLPSGRNLAYARAKVEPGDYGDKIIYEGQGDRAYFTAQETYGGKLVE
                     NIVQATARDILAEALLRIEAAGYDVVFHVHDEAIIEGSGLIIEEVNDLMAQAPEWAEG
                     LPLNSEGYVTKYYMKD"
     gene            35126..37510
                     /locus_tag="VHD02_000274"
     CDS             35126..37510
                     /locus_tag="VHD02_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEQ5496912.1"
                     /translation="MKQEKLIVKSSPLQELHIATGSSRTAKTWKNITLTWQELVERLE
                     KPTVTQETFAEYQKMSRAEKGQAKDVGGFVGGWLKQGKRKNENVQSRSLVALDADSPS
                     KDFLDRLDLLADYAFVLYSTHSHSKKAAKYRLIIPTDRLMMPDEYEPVARYLANQLGM
                     SNFDDTTYQSVRLMFWPSHSRDADFTFKYNDEAFLSVDEVLDTYPDWHDSSFWPESPT
                     HAVKRQREAKKQGDPLSKKGLIGAFCRNYDIRQAIATFLSEVYEEGTTPDRYTYTDGS
                     TANGLVIYDDVFAYSHHGTDPVGDTLVNAYDLVRIHKFGEQDSEAKDNTPTNKLPSSK
                     AMNAFVSDLPEIKDHLMAEALGDFDEELPVEDDRSWLEIDERGEPEVNSYLLATQIIK
                     EVPIYWDGLEFLRYDAKKGIWLPNAEEYLKSYISTKKLGKITKIRHISETVVAIKAQA
                     FSSEVFTESDLNKIVLANGVYDLRDNNFKTKFDPELHARSSHPVVYDPEATCETFEGF
                     LRETVGAENIDFIFEWFGYNFYREYTIQKMLFIYGSGGTGKSTLINILREMIGADNYS
                     AVTLQYLMQERFAKIGLYRKTANFDTDAKPQYLADGAALKMLTGEDTIHADRKNKEPI
                     NFYNYAKLSFAMNELPPMRDFSGGLKRRMMILEMDKVLTQEVKAKYPLDKIMSEVPGI
                     FNRAMEGLRKALSKRDFSISASMRSSVEKWEKGNDVVAMFLEDECELGEDFKVPVRDV
                     YPAYKFYCQDSGYKPLAKNAFNHRLRELNYENKNVKSDGKQAKNWVGFRLKSEF"
     gene            37897..38172
                     /locus_tag="VHD02_000275"
     CDS             37897..38172
                     /locus_tag="VHD02_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEQ5496913.1"
                     /translation="MRTEKDIENYLKKKTKGLCLKFTSPGTIGVPDRIVVMNTGTFFV
                     EVKAPGKKPRPSQVAMHKKIKEAGQHVWVVDSYESVDMALKEMENWV"
     gene            38169..39491
                     /locus_tag="VHD02_000276"
     CDS             38169..39491
                     /locus_tag="VHD02_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEQ5496914.1"
                     /translation="MRLHEYQEYAKTWIVEHPYCGLLLDMGLGKTLTTLSAIDEIQNI
                     FSEDHKILIVAPKKVAEETWPTEIEKWHFDFTYSKVLGSEGKRIEALETEADIYLINR
                     ENVTWLVEYYKTKWPFTFVVIDELSSFKSSKSKRFRALRKVRPKVQRLVGLTGTPAPN
                     SLIDLWQQIYLMDRGDRLETSQTRFKDKYFVPDKRNGPIIYSWALRDGAEAEIYNKIE
                     DICVSMKAKDYLKLPPRTNNVVSVKLSNMKAYKQLEADLVLEFKDKEISAANSAVLAN
                     KLLQMANGAIYDDDKSTVAIHDDKLDALEDIVEESQGQPILVFYQYQHDFERLKKRFP
                     QAEELTSVDKWNSGKIPILLCHPQSAGHGLNLQKGGHIIVWFGLTWSLEYYQQANARL
                     DRQGQTEPVIVHHIVAENTVDEKVLRILQGKEKNQNALLEAVKAQLGV"
     gene            39492..39659
                     /locus_tag="VHD02_000277"
     CDS             39492..39659
                     /locus_tag="VHD02_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496915.1"
                     /translation="MKKEYVVRIYTGREKNFEAKPQFEEKTFTRKADMLKFWNSCEAT
                     VKEKYTREWKK"
     gene            39656..39922
                     /locus_tag="VHD02_000278"
     CDS             39656..39922
                     /locus_tag="VHD02_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496916.1"
                     /translation="MTEELIKPLLEEIKRKDDRLYMQSNVIAVLMTAVIMLLMISIAL
                     QNHYEPQITGLRTQLSRTQKQLKRASEDRARQTKRIAELTGNGG"
     gene            39931..40782
                     /locus_tag="VHD02_000279"
     CDS             39931..40782
                     /locus_tag="VHD02_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEQ5496917.1"
                     /translation="MEIKCGNCLELLAELTDGLIDMVITDPPYNISVKNNFATMGRTG
                     IDFGDWDKGFDLLSWIDIASQKLTKNGGMIIFNDWKNLGDIARHCEKNGLVVKDIFRW
                     VKDNPMPRNRDRRYITDSEYGVWVVRKKSKWVFNRKSEKYDRPEYRYPVVAGAEKTQH
                     PTQKPVALMKDIITRHTTKGAVVLDPFMGSGSTGVACLLTGRDFIGYELNKDYFNIAN
                     KRIAELTGNGGVGMNIIHRMENGIDAVGDLTTYAVPCCPECKNETYSQKHCPFCGENL
                     MEVANEN"
     gene            40772..40963
                     /locus_tag="VHD02_000280"
     CDS             40772..40963
                     /locus_tag="VHD02_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496918.1"
                     /translation="MKIEFFLKQGPTKSVSNYGEICIDEVIGAMKNNDVIALNGKNNA
                     VIIPKQNISHVIIHKEVTG"
     gene            40960..41376
                     /locus_tag="VHD02_000281"
     CDS             40960..41376
                     /locus_tag="VHD02_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF722 domain-containing protein"
                     /protein_id="HEQ5496919.1"
                     /translation="MSRLSNAQLKAFDEWLFDYRFIDNKIALRKLELQTDMNTEVDKN
                     IGGGRANFVSKVTEDVVARWDSDRELKGLSNFKEAVIATLNLLDEELTDIFNLRWGVG
                     SSNTWEEIAYTRHLSRKSIYRKRERILEIFSKKIGM"
     gene            41466..41918
                     /locus_tag="VHD02_000282"
     CDS             41466..41918
                     /locus_tag="VHD02_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEQ5496920.1"
                     /translation="MLGGDGKLSKLTLKQKRFADEYIISANATAAAIKAGYSKKTARS
                     IGQENLTKPDIKAYIDERLEKLESEKIATQEEVLQYLTSIMRGDQQEKTLISVGEFGQ
                     KIVDIDVGAKDRIKAAELLGKRYRLFTDKVEMDVSSDVTINVGEWDDD"
     gene            41908..43185
                     /locus_tag="VHD02_000283"
     CDS             41908..43185
                     /locus_tag="VHD02_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEQ5496921.1"
                     /translation="MTIKQRPKINIVIKYPSKVFNKHIYDKLYNYSNFTEVHYGGASS
                     GKSHGVFQKIILKALNPKFKHPRKILVLRKVGATVRDSVFADIMSNLSYFGILDKCKI
                     NMSAFRITLPNGSEFIFKGMDNPEKIKSIKGISDVVMEEASEFTLDDYTQLTLRLRDK
                     KHLEKQIYLMFNPVSKVNWVYKAFFVKTPKNTVVYQTTYKDNRFLDDVTRENIEELAD
                     RNEAYYKIYALGQFATLDKLIFPKYDKQILNKDKLSHLSSFFGLDYGFINDPSAFLHV
                     KIDDANKKLYVIEEYVRKNLTNDKIANAIKDLGYAKEEIRGDSAEKKSNQELRNLGIP
                     RMIDVTKGPGTVMQGIQYLLQYDWVVDERCVKTIEELENYTWKKDKKTNEYINEPVDS
                     YNHCIDAIRYAVQDRIYQSADRSKRMKNAKYYF"
     gene            43201..44733
                     /locus_tag="VHD02_000284"
     CDS             43201..44733
                     /locus_tag="VHD02_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEQ5496922.1"
                     /translation="MEEKQFLAGTRFNENANKQFIMLQEDFEAIDYESKLWIDQLKNY
                     ISRFKAEQLERLKELKRYYLGDNNIKYRPAKTDKYAADNRIASDFAKYITVFEQGYML
                     GVPVEYKNENKDLQAAIDLMSVRNNEDYHNVKIKTDLSIYGRAYELLTVEEIDDKKTE
                     VKLYQLPAEQTFVIYDDTYQRNSLMAVHFYNIDYGSGKRKQIIKVYTSDTIYTYEDYN
                     LETKGTCLKEHEEHFFKGVPVNEYANNEERTGAYESVLDNIDAYDLSQSELANFQQDS
                     VNALLVIAGNAYTGADENDYLDDGRLNPNGRLAISIGFKKAQVLILDDNPNPNGVKPQ
                     AYFLKKEYDTAGSEAYKNRLVADILRFTFTPDTQDMKFSGVQSGESMKYKLMASDNYR
                     EKQERLFKKGLMRRLRLAANIWAIKGNEATTYSLVNDTSIVFTPNLPQNDNEIVTAAQ
                     NLYGIVSDQTIFEILNTVTGVDAEAELERLKEEADKKQSLPEPRLVGDASGQEEPTAE
                     KP"
     gene            44693..46138
                     /locus_tag="VHD02_000285"
     CDS             44693..46138
                     /locus_tag="VHD02_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEQ5496923.1"
                     /translation="MLVVKKNQQQKSLNYWQKRQEDILSYLDRADLDVFSELQKLYNE
                     QAFELQKELFDFYTKYSEENKMTYQDVVKKLRHEDLSDYVANANKYRKQAEKDPELLK
                     RLNEQYVSARATRMDALNLELVYRAGILKGVLDSAFENHLKKAASYAYKKAMGGRSGT
                     INGPVLEELVRTPFDGYNYSEQLWGNTDNLVKNLQKKLKQGFVRGEHPRAIARDLAKQ
                     FNVANHRAETLIRTDGTMVINNATARRYLDAGLKYYRDLVRRDDRTTEICRKIAKENK
                     RKLLSELKPGINAAPYHFNCRTTLIPDEDELSIEVEPIDDKSTKYFKDVTSDWIDGNG
                     HKPQLSLLNEYVKNGTPYKVDGHNVVLDHSNYEYRVANWLSKKTGLQVDMVPRVNSPE
                     HINTPDYLVDRVPFDLKEITGSGKNVIDGNLRKAKKQATNIIFDITKTPLSFEEIMGQ
                     LEQIYMIDRRGLDISIIKNKDEVLAVLEKEG"
     gene            46169..46357
                     /locus_tag="VHD02_000286"
     CDS             46169..46357
                     /locus_tag="VHD02_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496924.1"
                     /translation="MGVRPSSFFISIIHHPIKTIKEENMFIWQMVLVALGVLVLILIV
                     GIAAIAVKSIIAELKKVG"
     gene            46360..46626
                     /locus_tag="VHD02_000287"
     CDS             46360..46626
                     /locus_tag="VHD02_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496925.1"
                     /translation="MNKRIKKKRKLETVVVMLVAENAMQAEAIKNQNKEIMELKSIVQ
                     RNALATNEEFASVKAATLDNQAAITNIAIDVNYIKKNYKRKWGK"
     gene            46782..47351
                     /locus_tag="VHD02_000288"
     CDS             46782..47351
                     /locus_tag="VHD02_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEQ5496926.1"
                     /translation="MAEENVTTETTEQVDTQKEAVEHPKQEHERTFTRADISKMMAAE
                     RTKWESEQSEAIEKARTEGERLAKLSKDERAKEEEQKRLDAIAEREKAVAEREMRIET
                     HSLLVEKGLPLDFIDIVLTTTAEEVKANIDNLQTIFDKAVEKRVNDRLTQKPPRTGNG
                     SVGMTKADIMAIEDDDERMRLIAENRNLF"
     gene            47364..48251
                     /locus_tag="VHD02_000289"
     CDS             47364..48251
                     /locus_tag="VHD02_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEQ5496927.1"
                     /translation="MAEKNLNTMADLGDIKSIDFVNKFSKNINDLLKLLGVTRRETLT
                     NDLKIQTYKWEVTLDQTDPGEGETIPLSKVTRTKDKDYTVKWFKKRRATTAESIARHG
                     AARAITEADKRIMRELQNGIKDAFFTFLKTKPTKVKGVGLQKALSASWAKLATFNEFE
                     GSPLVSFVSPLDVANYLGDTKVGADASNVFGMTLLKNFLGMQNVIVMPSVPEGKIYST
                     AVENLVFASLNVKGGDLGGLFADFTDETGLIAAARNRQLSNLTYESVFFGANVLFAEI
                     PEGVVEATIEAAAVPGIGG"
     gene            48263..48619
                     /locus_tag="VHD02_000290"
     CDS             48263..48619
                     /locus_tag="VHD02_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEQ5496928.1"
                     /translation="MGDKQLIDDIKLFIGISKDDGAQDELITLAISESKERVLAKLNE
                     YSETEITKIPDRLRFIVRDVAIKRFNRINSEGTVEDSEEGKTFKWDSYLKEYESTLRS
                     AAIGKVYSGKGVARFI"
     gene            48630..48908
                     /locus_tag="VHD02_000291"
     CDS             48630..48908
                     /locus_tag="VHD02_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496929.1"
                     /translation="MIYKDRVILVYVDEQDDFLDKRTVEKPSGKIPCMENTFTKSEQM
                     GLFGKYDLNAFKLHLQGHYDGFSKIIYKGKPRLIKGLAHHKNSTVIYV"
     gene            48905..49249
                     /locus_tag="VHD02_000292"
     CDS             48905..49249
                     /locus_tag="VHD02_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEQ5496930.1"
                     /translation="MSLIYRMRGLDRFLRSVERKQKSVRIAVDKELSKSAARIERQAK
                     ILAPVDTGWLRAQIYNEQQRLLHYRVVSPALYSIYLELGTRKMEAQPFLDPALRKEWP
                     VLMANLKKMFKR"
     gene            49253..49612
                     /locus_tag="VHD02_000293"
     CDS             49253..49612
                     /locus_tag="VHD02_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496931.1"
                     /translation="MDYSPETLYLKKVKNRLGALGIPIYFKLPKSDVLEPFIVVGTNI
                     SDLSKTAQTGAVIDDFSLNIDAFLPGDSRLDAEEIKARLLRLLGRNNQIKAQILVDNS
                     IGREVYRVAINITETLF"
     gene            49624..50223
                     /locus_tag="VHD02_000294"
     CDS             49624..50223
                     /locus_tag="VHD02_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEQ5496932.1"
                     /translation="MANTKEGTTIEITTGKPIVGKKIFYFIQSVDAKKGSKALLPAYR
                     TDGTTTMGGEYIDEQTQQGRVIEKATDEHSIDLTTYFVPTDPSVAVIEEAKKTGKSIK
                     IWEVIADESVKEQIQISESAGPKKDVYPAKFGYAKIDEIERGTGISDLVEMSYTANIV
                     GALQDGKFPLTKEEIEMLENVYGYQNPGDTTGDYDNITK"
     gene            50277..50732
                     /locus_tag="VHD02_000295"
     CDS             50277..50732
                     /locus_tag="VHD02_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496933.1"
                     /translation="MEFTAAKRNIDIKFDFKTMFKINNKLGTINPETGERNADGVGAL
                     FFNILERNESAIVDLVRLSAGSGKKALTEDEILDAIAEAVDEEGTTEGLFAEIEKEMV
                     DSGFFRAKILKYIENMEKSARYLKAKDDTDATQIQIIEDMIGRMSNAVS"
     gene            50807..51040
                     /locus_tag="VHD02_000296"
     CDS             50807..51040
                     /locus_tag="VHD02_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496934.1"
                     /translation="MEGLEYKQVAERENLSELSLKLRYTLNAKKVDVGKLKYDKHRLT
                     IKRSYQKASRSQADSDSSIVERIQMLNNHFQNR"
     gene            51055..55437
                     /locus_tag="VHD02_000297"
     CDS             51055..55437
                     /locus_tag="VHD02_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEQ5496935.1"
                     /translation="MPGTFDGSIFADIGANTKDYEQAMARIVSTTQNAFRKAQDTAVN
                     SSNKMVQIIGQIMAQLANNGESLGKRLGSAYATGLKLSIGEIQRIAASIGEKIPEPIK
                     NGFQKIIIPVASVIDKIRGNLTGFINQTKMQLQNLVDIGKIRSAFAKAATGVDILTQR
                     ASSKLNNLSAIFETVAGKLPRPFGTAFRKIASSVSTLNSSIQSVGGKISNSLGRQVLN
                     PALQSWNNFFSSVSAKASAFANKVSTSLFGRLTSSLANLSSKIGSSLSNGFSRMSSSA
                     ATSLNGISQKFANTSSAGERLKSTVMSIVQAFSLMAVVQKAMHAITGAIDGAVSRVDT
                     MNRFPKTMALFGYSAEQSKASIDKLSKGIEGLPTPLDSAVKSAQQLAITTGSLDKGTS
                     LALAFNNAMIGYGATTEGAEQALRQFNQSLGSGKIQAEEFNSVSEAAPGLMSKMAEAF
                     GFGKNGVQDLKSALSDGKITAQEFADKMIELNDAQGGFAEMAQSSAGGIRTAWKNVHT
                     AVVKGVAGMISAFDEAAKANGMKTIAETLLSLKPAITSVFDTINSLIPNAVAAFARLK
                     QSINIDFSPLGASVKEVFALINTVLGDFAHTGELSGQAFDNLKAKITSLAPKVIALWA
                     VMNPASAIATIMPLLSLFGKVGLALGSLGASVGVFGGIISSGIASASGVVGAFAATLS
                     GLPGVFATAAGRGLSVLGTMTSAMSSLVSLALAAIGPAAILGLVVAGLGLINSQFGAQ
                     IDQLLNTAVTKGPSIIQGLVKGITSKIPALIASGTQLIAKFANAITVLLPVIIQAGVQ
                     LITSLVQGIGQNATSLISSAIKIIGSFVSSIASALPQLISVGMELLLNVVNGIVQNIP
                     LIIQQAQQIIGSFGNSLQANLPSIISHGIAILVNLVQGITQMLPTVLQIATQVITSFV
                     SGIVQFLPQLLQGGIQIIISLVQGIIQNLPQIVQSAVQIIQSLVSGLTQALPQIIAAG
                     IQLVVQLAVALIKGLPQIISAGIQLITGLGKAMLEAIPNALSGVWEGIKSGFSSMWDQ
                     ITGKSSTSTAKVSADATAMALNVGTQTTAMANQANTDTTSMLNSISQNTELANINATS
                     NATQMANNVNAQTGTMSLQGLNDSMSLASGISANMSVANINATTQAQEMASGVNGATS
                     SMNLDAINHTLSLASGVGANMGTASTSATSQAQAMSSGVSNSLASMQSNSTKAASGLS
                     NSVTSEMSSAATSATSSANRLSSAVESGFNKAKTSATTSMNGIANAVKTGFSSINSTA
                     KQSMANLVSSVTSGMSRATAVSNNACQKILSIFRALAGQMSSAGAYAGQGFANGLASS
                     AGTIYAIANSIAANVAATIRRALDIHSPSRVTKTLGAFTGEGFALGMAEWIGEINSLG
                     KAYATAVTDQNWGVNSTVSTSAKVNNSGINTSLDNLSEEVRQSQLSEPIFEVHNEIVG
                     DKIYTAVKEKESREQSKDSYFVFA"
     gene            55449..56291
                     /locus_tag="VHD02_000298"
     CDS             55449..56291
                     /locus_tag="VHD02_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ5496936.1"
                     /translation="MDLLIEKESQATRLSDFGIYNIAIEDSAPLLSVSHRAVKGRSGY
                     IYDGATFTTKTLKVKGRVTVSDVEGVLDKQDELNALLVVDEPFYVTKMYPENSDLFNF
                     ELPGESTGDLQLIGSPHKPWKYRFKVILDDTIAYEFIGKTSQGLKYNLSFTLRTAELP
                     FGETKPKDITLSGGSFAYGGTAKASQLEWPFIIELTPSGGQTNFYIEIDGRRFEFKQN
                     SQLQNSDKLLLTGIATTLNGNYVNAKTNYEYFIFNPNPNKRITYKTDFLGTIRILNFV
                     ELYK"
     gene            56301..58283
                     /locus_tag="VHD02_000299"
     CDS             56301..58283
                     /locus_tag="VHD02_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ5496937.1"
                     /translation="MITFLDHRDIEYGAISVIRHTNAVNGERSVSGEIYTNSDVLNNI
                     DRGWRLRFEDEYYVVIYAKPVDVGQKTQVSFDAIHQFFWDFSKSSIYEGLGDGSHTID
                     TYLETVFKGSGYRYKLEVGVNAFRKQSFNYKSRLDLFNEIIKATGLEFSVSGKVVRLL
                     KNIGTDLSTVVRKNFNMNDLTIEKNIDSFITYQKGFGAWTDPEDHSKGRLEAEYESPL
                     AKEYGRLEGAPLTDERFTVADNLKEALKSNVENSYKISVKIDMEDLMRAGYRCERPVA
                     GDYIMAINETLGFQERIRIVSFTSYYDATGALVKHEVTCNDIGSVKKQSVGSLSINSR
                     INQIDADIASAIEVATQALVSADGKNTVYGGTEMPKDEPKGTLKKGDILFLKVGDTTR
                     MYFWNGAEWEEPEVVNDPERWREDLEKQISEAIEKAKKAQEEINRRTDKELEEFRETL
                     KNLALPEEAIKKITDAIKVDDIPSIKQSFDDLKNKVSETSETSRLNAEIIGTDGKTRY
                     NKNLLVGNPNRVKKIDEDFIEVEANDGGFKRGETYTISFSQTCEPLNKVAITLIQANN
                     KGLKLVLTPTKAKMEPQTFEVTKDKQSIEVYPLSYKGVLTGDWYKSKQIDLTASEGQI
                     LALDMAYKAVVDAKGATITAKQSSNPKIILDGRRDR"
     gene            58280..59335
                     /locus_tag="VHD02_000300"
     CDS             58280..59335
                     /locus_tag="VHD02_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronoglucosaminidase"
                     /protein_id="HEQ5496938.1"
                     /translation="MTLEERIPIKVLFDRKDAAEWQKLNPVVDDGELVVELDTHRLKV
                     GDGKLNYNDLPYYEGPQGESITKVQLSENGDLSVWIGDKETKLGNIKGQKGDKGTSIA
                     DITKVGETLTITLSDDTQKTFSIPNGQKGDRGKGVESARVDEEGNLFVKFEEESEKLV
                     GNIKGQKGDKGDSLKFEDLTSDQIAQIKAKDVDLSAYATKAELKEIDVSKQLADYLSK
                     AEANSTYAKASHKHSLSDITDLNLDRYATKIELQNKASTYHRHRTSDIDGIDEYLKQT
                     DLPQDIVKQSDIRDVVRNAQLSGYVRLADIQYQLNNIGKLKDVATGQYLSVRVVDKGK
                     VPYNTSGLIVFERSGGK"
     gene            59335..59970
                     /locus_tag="VHD02_000301"
     CDS             59335..59970
                     /locus_tag="VHD02_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496939.1"
                     /translation="MAVQEIPDTYFYRLDSQSIDGTLLQKASLVVDQFYIKIDDYYSF
                     YTDQDVDVYKIISSTQADVYKANKYNYQSLSKPDTSVQKPSAAMGLYQSITIDGLPIA
                     RVICNNQVIWSQSAEPQEVYNITAYVDANVSKLNDYAVIDYNQDFATYGVVWHRVDKV
                     AVEVDGKIVAKLQKPVRTVAGGGAMLSIETWTYPFDGIDKYKNPTFKISQH"
     gene            59981..61888
                     /locus_tag="VHD02_000302"
     CDS             59981..61888
                     /locus_tag="VHD02_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp58-like family protein"
                     /protein_id="HEQ5496940.1"
                     /translation="MSRDPTLLIDESNLTIGSDGRAYYTFKADGDTKSVKLANNKCIG
                     TTRFNQLMIERGGKPTNYVAPVVVEGSGESTGLFKNLEGALSQLKELNLELTDTVNSQ
                     LWAKIKLTTNGMLREYHRDNITTEIVESANGIATRISEDTDKKLALINETVSGIRRDY
                     QDADRQLSSTYQAGINGLKATMANDKNDLKAVIQATAQGLSQKYDDEIQQLSAKITTT
                     SSGTTEAYESKLADLRAEFTRSHQGMRTVLESRISGLQSTQQSTAYQISQEIKNREGA
                     VSRVQQDLASYQRRLQDTEKNYSSLTQTVRGLQSTVSAPNRGLESRVTQLAGLIEQKV
                     TRGEMESYIRGAGDSIMLAIKGKLPQSKMSASEIVSAINLNGYGVRISGERIALDGNT
                     TVNGAFGAKLGEFIKLKADQIIGGTIDANKINVINLNASSIVGLDANFIKARIEHTIT
                     SLLEGKVIRARNGAMMIDLNNSTIDFNSDASINFNSNNNALVRKSGTHTAFVHFGNAT
                     PKNFTRSALYASIGITSSGDGINSASSGRFCGARFFRTASGYEHTASVDQAEIYGDTI
                     IFADDFGINRGFKMTPTGVNTLVDINKMYYAIVALARCWKHLQNVGWDTTHPNFTSAI
                     MNEHSNYMTGI"
     gene            61897..62058
                     /locus_tag="VHD02_000303"
     CDS             61897..62058
                     /locus_tag="VHD02_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496941.1"
                     /translation="MQEKLLGKIINDLTLKVANLTLENAQLKAQHEIELEELNAQLDE
                     ATAPKEEGK"
BASE COUNT        19665 a        10874 c        13667 g        17852 t
ORIGIN      
        1 tgattatcaa atgattttaa agagaaataa tttcttagtc aaataaatta tcagaaaaga
       61 ggattgttat gcagtttaaa cacatcatct ttgatgttgt agatgatctt gcgatattga
      121 ctttaaaccg tccagaggtt tcaaacggtt ttaatattcc aatttgtcaa gagattctgg
      181 tagcattagc agaagttaaa cgcgatacat ctgtgcgttt tttactaatt aaagccgttg
      241 gaaaagtgtt ttcagttggt ggtgacctag ttgaaatgca agaagctgtt gcaaaagata
      301 atgtgcagtc gctagtcaag attgctgaac tggtacaaga aatttctttt gcaatcaagc
      361 atttgccaaa accagtcatc ctttgcgccg atggagctgt agctggagcg gcttttaata
      421 tagctttagc tgttgacttt tgtatcgcta gtactcaaac aaaatttatc caagcttttg
      481 taaatgttgg actagcaccg gatgccggtg gtttatttct attgactcgt gcagtaggac
      541 ttaatcgtgc aactcatctt gtgatgacag gtgaaggaat tactgctgat aagggtgtcg
      601 attatgggtt tgtctaccgt acggcagaat ctgataaact agacaaagtt tgtcttcaac
      661 ttttaaagcg attgagacgt ggctcttcaa attcttatgc tggaatgaaa tcattggtct
      721 ggcaaagctt tttcacagga tgggaagatt atgctaaggc agagcttgct attcaagagg
      781 agctagcttt caaggaagat tttaaagaag gtgtcatagc ttttggagaa aggcgccgac
      841 caaattttca aggtaagtag actcatgctt gcaaagtgct tttaactctg atataatttg
      901 actgtcaaag ttttattgtt aggaggtatt tgcttggaat atgacaaaat caatcactac
      961 ttggttgata tcttcaaccg tattttggta attgaagaaa tgagtttaaa aaccagtcaa
     1021 tttaatgatg tctctttaaa agagatgcac actattgaaa ttatcggcaa atatgatcag
     1081 gtgactccaa gtgatatcgc cagagaatta atggtaacgt tagggacagt aacgactagc
     1141 ttaaataaat tagaagcaaa aggttatatc gcaagaacgc gatcacgctc agataggcga
     1201 gttgtttacc tgtcattaac aaaaaggggt cgacttttgg accgcctcca tgcaaagttc
     1261 cataaaaata tggttggaca tgtcattgct gatatgagtg atgaagagat gcaggcattg
     1321 gtgcgtggac tagggaatct acatcaattt ttggaggatt tggtttaaat gattttttct
     1381 aaaatcagtc aggtagccca ctatgttcca cagcagttag tgactaataa tgatttagct
     1441 agcataatgg acactagtca tgagtggatc ttttctcgaa caggaatagc agaacgccat
     1501 atttctagag atgaaatgac tagtgaccta gctattcagg ttgctgatca gcttttaact
     1561 cagtctgggt taaaagctga tgctattgat tttattattg ttgcaactat cagccctgat
     1621 gccactatgc catcaacagc tgcgaaagtt caagcagcta ttgcggctac aagtgctttt
     1681 gcttttgata tgactgctgc ttgtagtggc tttgtctttg ccttggctat ggcagataag
     1741 cttattgcat caggtgccta ccaaaacgga atggtaatag gtgcagagac tttatctaag
     1801 ttagtaaact ggcaggatcg agcgacagcc gttctttttg gagacggagc tggtggcgtg
     1861 ctactggaag ccagtaaaga taaacatgtt ttagcggaaa cattacacac agatggtgcg
     1921 agatgtcaga gtttaatttc aggagaaact agtctttcat ctccgtactc aattggcaaa
     1981 aaagccatag cgactattca gatggatgga cgtgccatat ttgattttgc gattagggat
     2041 gtgtcaaaaa gcatcctcac actaatggcg caatcagata ttacaaaaga tgacatagac
     2101 tattgtttat tgcatcaagc taatcgtcgt atacttgata aaatagcacg caagattgat
     2161 gttcctcggg aaaaattcct tgaaaacatg atgcgttatg gtaatactag tgctgctagt
     2221 atcccaatcc tcttgtcaga ggctgttcaa aagggacaga ttaggttaga tggtacccaa
     2281 aaaatcttac tttccggttt tggtggaggt ttgacatggg gaagtctaat tgttaaaatc
     2341 tagttattca tgtgcctagc acatttataa aaacaaattt atatttttta aggagaaata
     2401 aacatggcag tatttgaaaa agtacaagaa attattgttg aagaacttgg taaggagaca
     2461 gaagaagtaa cactagaaac tacatttgat gatttagatg cagattcact tgatgttttc
     2521 caagttattt cagaaattga agatgctttt gatattcaaa ttgaaacaga agaaggatta
     2581 aatactgttg gtgaccttgt tgcttacgtt gaggaaaagt caaaataagg ataaaagtga
     2641 caagtaacat cagaagtcta tgaaaagctt actgctgtta tgttcatgtt catcactaaa
     2701 aaaaacaata agtattaatc aaaccattag ggtgggatta gactcttttt aggatctagc
     2761 tcgctctttc ttcttactat aatagtttga ttatcaaatt attctcctta ttttttatag
     2821 aaagttatta tgaaaacacg tattacagaa ttacttaata ttgattaccc catttttcaa
     2881 ggaggaatgg cttgggtcgc ggatggtgat ttagcaggtg cagtttctaa tgctggtggt
     2941 ttaggcatta taggtggtgg caatgctccc aaagaagtcg ttaaagctaa tattgatcgt
     3001 gtcaaagcta ttactgatag accttttggg gttaatatca tgcttttatc tccttttgct
     3061 gatgatatcg ttgatttggt cattgaagaa ggtgttaaag tagtaacaac aggcgcagga
     3121 aatccaggaa agtatatgga aagactgcac caggcgggta taatcgttgt tcctgttgtc
     3181 ccaagcgttg cgctagccaa acgtatggaa aagcttgggg tagatgcagt gattgctgag
     3241 ggtatggaag ctggaggaca tattggcaag ttaacgacta tgtctttagt aagacaagtt
     3301 gttgaagcgg tttcgattcc tgtcattgcg gcaggtggta tagctgatgg tcatggtgca
     3361 gcagcagcat ttatgttagg agcagaggct gttcaaattg gaactcgctt tgttgttgct
     3421 aaagaatcca atgcccacca aaattttaaa gataaaatct tagcagcaaa agatattgat
     3481 acggtgattt ctgcgcaggt tgtgggccac cctgtccgtt ctattaaaaa taaattgacc
     3541 tcagcttacg ctaaagcaga aaaagcattt ttaattggtc aaaaaacagc tactgatatt
     3601 gaagaaatgg gagcaggatc gcttcgacat gctgttattg aaggcgatgt agtcaatgga
     3661 tctgttatgg ctggccaaat tgcagggctt gtgagaaaag aagaaagctg tgaaacgatt
     3721 ttaaaagata tttattatgg tgcagctcgt gttattcaaa atgaagctaa gcgctggcaa
     3781 tctgtttcaa tagaaaagta gataaacaca tgaaggataa tgataatgac aaagacagcc
     3841 tttttatttg ccggtcaagg tgcccaaaaa ttagggatgg caagggattt ttatgataac
     3901 tttgctattg taagaaaaac ctttgatcaa gctagtcaag tattgggata cgatttgcgc
     3961 cgtttgattg atagtgacga gttaaaactt aatcaaacta gctatacgca accagctatt
     4021 ttgacatcat caattgctat ttaccgtgtt ttgggcttac atcacgttaa accggatatg
     4081 gtagctggtc tttccttagg agagtactca gctttggtag catcaggggc actctctttt
     4141 gaagataccc tatccttagt agctaagaga gggcgcttga tggaggaagc agcaccacaa
     4201 ggatctggaa agatggttgc cgttatgaat acagacgtgc aagtcatcga agaagtctgt
     4261 caaatagctg ctaaacatga agtggttgct ccagcaaact ataatactcc tagtcaaatt
     4321 gtaattggtg gtcagacaga tgctgtgaac gtggcagttg aacttttaaa ggaaagggga
     4381 gttaagcgtt taatcccttt aaacgtgtca ggtcctttcc acactgcttt gttagaacca
     4441 gctagccgct tgttggctaa agagttggaa agatacaact tcagtgactt caagattcct
     4501 ttggttggta ataccgaagc taatattatg gaaaaagacc gtatcccaga actattagcc
     4561 cgtcaagtca tggagcctgt tcgtttttat gacagtgttg cgactttagt agaaagtggc
     4621 ataacacaat tcattgaggt aggaccaggt aaggttttga caggttttgt gaagaaaatt
     4681 gataaaaatt tactatgtac tagtgttgaa aacatggtaa gcttacgttt atttttagat
     4741 agagaggaca ttcatggaaa ttaaaggaaa aaatatattt atcacaggct caacgagagg
     4801 tatcggttta gcaatggctc atcaattcgc cagtttagga gctaatattg tcttaaatgg
     4861 tcgctctgct atctctgagg agcttgttgc tagttttact gactacggcg ttacagttgt
     4921 taccatctct ggggatgttt cagaagcatc agaagctaaa cgtatggtca atgaagctat
     4981 cgaaagttta ggttcgattg atgttttagt taacaatgct ggcattacta atgataaatt
     5041 gatgcttaaa atgacggaag aggattttga gcgcgtcttg aaaatcaatt tgaccggtgc
     5101 ttttaacatg actcaatcag tgttaaaacc aatgatcaag gcccgccaag gagcaattat
     5161 taatgtctct agtgtggttg gtttaacagg caatattggg caagcaaact atgccgcttc
     5221 taaggcagga atgattggtt ttaccaagtc tgttgcccgt gaagttgctg ctcgtaacat
     5281 ttgcgtgaat gctattgctc caggatttat cgagtccgac atgacaggtg tccttcctga
     5341 aaaaatgcaa gagcaaatct taagtcaaat cccaataaaa cgtattggaa aagctcaaga
     5401 agttgctcac ttagcaagct tcttggtgga acaagattat attacgggac aagttatcgc
     5461 tattgatggc ggtatgacaa tgcaataagg agacgtagtg aaatgacatt taaaagagta
     5521 gtggtaacag gttatggtct aacatctcca atcgggcatg atcctgaaac gttttgggac
     5581 aatctgaaag ctggccaaat tggtattggt cctattacta aatttgacac gactgattat
     5641 gcggtcaaga atgctgctga aattcaggat tttccatttg acaaatactt tgtgaaaaaa
     5701 gatttgaacc gtttcgatag atattctctt tatgctcttt acgcagcgaa agaggctatt
     5761 aatcatgctg atttaaatat tgagatgatt gattccgatc gatttggcgt tattgttgct
     5821 tcaggtattg gtggaattgc ggagattgaa gagcaagtga ttcgcttaca tgaaaaagga
     5881 ccaaaacgtg tgaagccaat gacgttgcca aaagcgctac caaatatggc tgcaggaaat
     5941 gtcgccatgt ctttaaaagc tcaaggagta tgtaaatcaa ttaatactgc ctgcgcttct
     6001 tcaaatgatg cgattgggga tgcctttcgt gccattaaat ttgggactca agatgtgatg
     6061 atagttggtg ggtcagaggc ggccataacc aagtttgcca ttgctggctt ccaaagttta
     6121 acagctctct caacaactga agatccaagc agaagctcta ttccatttga taaagaccgc
     6181 aatggtttta tcatgggaga aggatcagga atgcttgttt tagaaagcct agaacatgcg
     6241 caagaacgcg gggcgacaat cttagcagaa attgtgggtt atggaaatac ctgcgatgcc
     6301 taccatatga catctccaaa tcctgaggga ttaggagctc gtaaagccat ccacttagcc
     6361 ttacaagaag ctggtattga agccagtgct attaattatg tcaatgcgca cggaacttca
     6421 accccagcta atgaaaaagg agaaagtcaa gcaattgtag ccgttcttgg taaggatgtt
     6481 ccggtgtctt caacaaaatc gtttacgggt caccttcttg gagcagcagg cgctattgaa
     6541 gctattgcca ctatcgaggc tatgcgtcat aactatgttc cgatgacggc tgggacacaa
     6601 gctttatcag aggatatcga agcaaatgtc atctttggag aaggaaaaga aacagctatc
     6661 aactatgcca tttcaaatac ttttggattt ggtggtcata atgctgtatt agctttcaaa
     6721 tgttgggagg agtaggttga atattcaaga aatcaaagat ttgatggctc agtttgatac
     6781 ctcaagccta cgtgaatttt tattcaaaac aaatgaaggt gagctaatct ttagtaaaaa
     6841 cgaacaacac ctcaatgcat cgacatcaaa tcaagaacat gctgtgcctg taccacaggt
     6901 acaacttgtt ccgaatccaa ctgcttcgga agcgtccagt ccagcatcag ttaaggatgt
     6961 tcctgtagag gagcaaccac aagcagaatc ttttgtagca gaaggtgata ttgttgaaag
     7021 tcctttagtc ggtgtagcct atcttgcagc tagccctgac aagccaccat ttgtggcagt
     7081 cggagatact gtaaaaaaag gacaaaccct tgtgattatc gaagccatga aggtgatgaa
     7141 cgaagtgcca gcgccatgtg atggtgtgat tacagaaatt ttggtatcaa atgaagatgt
     7201 cattgaattt ggacaaggat tggtgcggat caaatgatgg atattagaga aattcaagcg
     7261 gctttgcctc atcgctaccc aatgttattg gttgatcgtg tgttagaagt gtcagacgac
     7321 catatcgttg ccattaaaaa tgtgactatt aatgagcctt tctttaacgg gcattttcct
     7381 cactatcctg tgatgccagg tgttctgatt atggaagctt tggctcaaac agctggtgtc
     7441 ttagaattgt caaaagaaga aaataaaggc aagttggtct tttacgctgg aatggataaa
     7501 gtgaagttca aaaaacaagt cgtccctggt gatcaacttg tgatgacagc aacctttatc
     7561 aagcgtcgcg gtaccattgc tgtcgttgaa gccagggcag aagttgatgg caagctagca
     7621 gcaagtggaa cattaacctt tgcctgtggg caatgagaat gattgttctg cggaggaaga
     7681 aacccttatg tttaaaaaaa tcttaattgc taatcgtggt gaaattgcag tgcgtatcat
     7741 tcgtgctgca cgagaattag ggatttccac agttgcagtt tattcggaag cggataagga
     7801 agctctgcac acgatattgg cggatgaggc tatctgtatt ggtcctgccc gttccaagga
     7861 atcctatctt aatatgaatt ccgtcttgtc agcagctatt gtgacaggag cccaagcgat
     7921 tcacccaggg tttggctttt taagtgaaaa ctcaaagttt gcgaccatgt gtgaagagat
     7981 gaatattaaa tttatcggtc catccgcatc ggttatggat aaaatgggtg ataaaatcaa
     8041 tgctcgttct gaaatgatta aggcaggagt gcccgttatt ccagggtcag atggtgaagt
     8101 ttataacgct caagaagcct tagctattgc caataagatt ggttatcctg tcatgctaaa
     8161 agcatcggca ggtggtggag gtaaagggat tcgtaaagtg gagacagaag cagatttgga
     8221 ggcggctttc aatgctgcca gtcaagaagc tttgggagct ttcggaaatg gcgccatgta
     8281 tttggaaaaa gtaatttacc cagctagaca tattgaggtg caaattttag gagacgctta
     8341 cggtaacatc attcacttgg gagaacggga ttgctcgtta cagcgtcata atcaaaaagt
     8401 cttggaagaa agtccatcca ttgctattgg caataccctc agacacgaaa tggggcaagc
     8461 agcggttaga gcagctaaag ctgtagccta tgaaaatgcg ggaaccattg agtttttgtt
     8521 ggacgaagac agcgagaaat tctatttcat ggaaatgaat acccgtatcc aagttgagca
     8581 tcctgtcacc gaatttgtga caggagtgga tattgtcaaa gagcaaatca aaattgcagc
     8641 aggtcagcct ttggcgatta atcaagaaga catcactatc acgggacatg ctattgaatg
     8701 tcgtatcaat gctgaaaata ctgcttttaa ctttgcaccg agtccaggaa agattacaga
     8761 cttgtatatg ccaagtggtg gtgtaggact acgagttgat tcagcagttt acaatggcta
     8821 tgccattcct ccttattatg acagcatgat tgctaaaatc atcgttcatg gaagcaatcg
     8881 atttgacgct ttgatgaaaa tgcaacgtgc cttagttgaa ttggaaattg aaggcattat
     8941 caccaataca gacttccagc tcgatttgat ttcagataag cgagtgattg caggagatta
     9001 tgatacctct ttcttgatgg aaacgttcct gccacattac aataaagatt aggagaaaat
     9061 atggctttat ttcgtaaaaa agataaatac atccgtatta ctcccaataa ttccctcaaa
     9121 ggatctgtca gccataatgt cccagaagtt ccagatgaac tctttgcgaa atgcccagct
     9181 tgtaagcaca tgatttataa gaaagatttg ggattggcaa agatttgccc aacctgttct
     9241 tacaatttta ggatttctgc ccaagagcgg ctgaccttaa cagttgatga aggctctttc
     9301 caggaattat ttacaagtat tgaaaccaag gatccgttac gttttccagg ttatcaggaa
     9361 aaattacaaa aagccaagga aacaactggt ctgcatgaag ctgttttgac agggaaggct
     9421 atggttaagg agcaaaagat tgctcttgct attatggatt ctcactttat tatggctagt
     9481 atgggaactg ttgttgggga aaaaattacc cgattgtttg aattggctat cgaagaaaac
     9541 ctgccggtgg tgatttttac ggcttcagga ggcgcccgta tgcaagaggg gattatgagc
     9601 cttatgcaaa tggccaaggt ctctgcagct gtgaaaagac attctaatgc aggcctcttt
     9661 tacctcacta ttttgacaga tccaactact ggtggggtaa cagctagttt tgccatggaa
     9721 ggggatatta ttttagcaga acctcaatcc ttggttggat ttgctggacg ccgtgttatt
     9781 gaaaccacag tacgtgagaa tttgccggat gattttcaaa aagcagaatt tttacaagac
     9841 catggttttg tggatgctat tgtgaaacga acagagttgc gagacaagat tgcccactta
     9901 gtagcatttc atggaggtgg ccaatgacag atgtatcaag agttttaaaa gaagcgcgtg
     9961 atcaagggcg tttaacaact ttggattacg ccaaccttat tttcgatgac tttatggaac
    10021 tgcatggcga tcgccatttt tcagatgatg gtgccattgt aggtggccta gcttatttgg
    10081 cgggacaacc tgttacggtc attggtattc aaaaaggtaa gaatttacag gataatttgg
    10141 caaggaattt tggccagccc aatccagaag gttatcgtaa agctttgcgc cttatgaaac
    10201 aggcagaaaa atttggacga cccgttgtta cgtttatcaa tactgcagga gcctatccag
    10261 gtgtcggtgc ggaagaacga ggacagggtg aggccattgc taaaaatttg atggaaatga
    10321 gtgatctcaa ggttcccatt atcgccatca ttattggtga aggaggctct ggtggtgcat
    10381 tagccttagc ggttgccgat caggtctgga tgcttgaaaa tactatgtat gcggttctta
    10441 gcccagaagg ctttgcttct attttatgga aggatggttc aagggcgacc gaggccgctg
    10501 aattgatgaa aatcacagcg ggtgaactct acaaaatggg aatagtagac cgtattattc
    10561 cagaacatgg ttatttttca agtgaaatcg ttgatatcat caaagctaac ctcatcgaac
    10621 aaataaccag tttgcaagct aagccattag accaattatt agatgagcgc taccaacgct
    10681 ttcgtaaata ttaacaacat ccttttggag tctccctacg tgaagggagg cctttccaaa
    10741 agggttttta actagtcttg taaataagga aatgggtata ctaataagac ttttgataaa
    10801 ctagtgtgaa gaaaagtgtt gttaaagggg caatgtagtg agtttcaagt gatgtggtgg
    10861 ctctttaaaa tagtaaaccc cagaaatcac tttaatgatc tctggggtga atttattatt
    10921 taggtgaaat gacggtttcg ccacccatgt aagggcggag tacttcaggg atggtaacag
    10981 aaccatcttc attttggtag ttttctaaaa tggctgctac ggtacgaccg acagcaagtc
    11041 ctgacccatt aagggtgtgg agaagtttga ccttaccgtc agcctcatca cggtaacgga
    11101 tttgagcacg tcgtgcttgg aaatcttcag tatttgaaca tgatgaaatt tcacggtagg
    11161 tgttttgtgc aggaatccag acttcaaggt cataggtttt agcagctgag aagcccatgt
    11221 cgccagtaca aagggaaatg acgcgatatg gcaaaccaag tttttgaaga atattttcag
    11281 cattagcagt cattttttct agttcttggt aagattcttc tggtttagca aatttaacca
    11341 tttcaacttt gtggaattgg tgtaggcgga taaggccacg tgtgtcacga cccgcagaac
    11401 ccgcttcaga acgaaaagat gggctcattg cagtaaagta aattggcaat tctttgccgt
    11461 ctaggatttc cccacggtaa taattggtca aaggcacttc agcagttgga atcaggacaa
    11521 agttggtatc tgctaattca aaggtgtcct ctttgaactt tggatattgt cctgtaccaa
    11581 acatggaatc gtggttaacc atgtaaggtg tgatgatttc ttgataacct tctttgatat
    11641 gttcatccag catgaagtta taaagagcac gctccaaacg agcaccaaga tttttataga
    11701 ataagaaacg agcaccagtt acctttgccc cacgttccca gtctaaaata tcaagatctt
    11761 cgcctagatc ccaatgagcc ttaacttcga agttaaagtc acgaggcgtt ccccaacggc
    11821 gaatttcgac attgtcttct tcgtcagctc caacagggac agaatcgtga ggagtattag
    11881 gtaaaacagt gatgatgtca gtaacttgct ggtcgatagc tactagttga ttgtcaatag
    11941 ttttgatatc tgcagagact ttttgcatat cagcaatttg ttgagtggca tcttcttttt
    12001 gacgtttggc ttgagcgatg gcagcagaag caatattacg ctctgctttt aattcttctg
    12061 attgcactaa tagagtacga cgtttttcat caagttcttt aagatgggtc agggtgtctt
    12121 cagaaacacc acggtttttt aatttggcag cgacagtgtc aaaatcagta cggatacgtt
    12181 taagatctag catacagaag ctcctttaag atagttatta gtagctgtca gtgaaacaag
    12241 gctaagtaag acagttggat cttgtgattc tctgacgata gaaaaagcac agcttatgaa
    12301 gatgctggag cgctttggcg cggttccatc cagcttcaca atctgtgcac ttaatctcta
    12361 ttttattgat gacacggtag aatttcatac cattttccct atctgttcac agcatccaca
    12421 gactctctga atgaaaaaag gtattactta tccgttagga ctattataac aaaaaacaag
    12481 aaaaagtaaa ctcaaatgag ggaatctttt ccactaaata gcaatttaga gctactattc
    12541 tttgttttct tgaaatgata aatcggcact gcgcattatt tttttaatgt cgaaaaaatt
    12601 tgttaataaa ggtttgcatc aaggttggta atgtaatcac tttctcatta ccaatatgtt
    12661 gacgagtgat ttttaagatt tttccagagt ctccagaagc aaataaaaac ttgccttggt
    12721 ccgtaaaaat ttcaaagtgg cggctaactt tttttcttga aacattagcc ccgatgtgat
    12781 tgatagctgt ccaaggaatt tgaatataat cattcacatt tttatcgttg taaaattcaa
    12841 gggccttgtc accgagtaaa atatgaccaa ctttgcctcc cattccaagg taggagacag
    12901 cttttgtctt gtattcgaca ctagtgttta aagattgagc cattttagtt cctttcattg
    12961 cgtagttgta gttattatac catattttaa gtgggatttt cctgacataa agcataaaaa
    13021 agaagcctga gttcatctca gacttccatt ggttgattac ataatacctg caaggtgagc
    13081 aaggatacca aatgcaaaca agccgatgat gattgcgatt ggagaaactt ttttcttcaa
    13141 taaccacatg caaaggaatg taaggagtaa tccagctaaa cctgggataa gagagtccaa
    13201 ttgtccttga agggtttgag cttggacttt atcaaggctc ataccgccaa tagcgttggc
    13261 aaggatagtt ttcaactcag cacctttaac agcgccatct gggaagacca cataggcacc
    13321 atctgacaat tgttttccag gaaggtcgat agtaaagttg atagataccc aacgttgaac
    13381 aagcactgca aggataaaca ttccaaggat tgaagcacct ttagtaatgt cttggaggat
    13441 accgcctgac atgtctttag taatttcaga accagctttg taaccaaatt cttgagtata
    13501 ccataagaag gccatacgga taaggttcca tccaaagaag aacagaagtg gaccaacgat
    13561 gttacctgtt gaagccaaag aagcaccaag tgccccaagg ataggacgaa ccgtaaacca
    13621 gaatactggg tcaccgatac cagcaagagg tcccatcata ccgattttaa ccccttggat
    13681 agccttgtca tcaattggcg taccatttgc gcgttcttct tcaagggcaa gtgttacacc
    13741 aatgattgga gctgcaacgt atgggtgagt gttaaagaat tccatgtggc gctcaagagc
    13801 agctgcacgg tcttcaggag acgtatacaa ttttttaaga gcagggataa gtgcataagc
    13861 ccagcccatg ttttgcatac gttcgtagtt ccaagaacct tgtaagaatt gtgaacgcca
    13921 ccaaacacgt tgacggtctg atttagttaa tttaatttgt tcagtcattt gttcttaccc
    13981 ctttctagta gtcttctaag atgtcgccga ttggatcagc tgaacctgct gcattaccac
    14041 cgttaccacc ttttttagaa agattaaggt agatgaaggc aattgctaca ccgataacac
    14101 ccatagcgat aagggttaat tggctaatag ctgcaagagc aaaacctagg gcgaagaatg
    14161 gccatacttc acgagtagcc atcatgttga taaccatagc ataacctaca gcaacaacca
    14221 tcgcaccacc gatttgcata ccttcgttca accagtttgg catagcattc aaaacagatt
    14281 gtacggctga agtaggaaca gcaacaagaa gagctgctgg gacagcgata cgcaatcctt
    14341 gaagaagaag ggcaataaag tggaagcgtt caatacctgc gaaattacct tcagcggcag
    14401 ctttgtcacc agcgtgtaca agggcagttg acaaagtacg aacgatcatt gtaaggaaaa
    14461 gaccagcaac agcaagtggg atagctgttg agtaagcaaa ggtaattcct ttttgagtaa
    14521 agtcgccact tttgaccatg atgatagcag ctgcaacaga ggcaagggca gcatcagggg
    14581 caacggcagc accgatgttt gcccaaccaa gagcaagcat ttgaagtgta ccaccaagga
    14641 tgacaccagc ttcaaggtgt ccagttacaa gtccaatcaa agtacaggca acaagtggtt
    14701 ggtgcatttg aaattggtcg aggatacctt caagaccagc gaagaaggca atgataacga
    14761 ccaaaattgc agaaataatc gaaatatctg acatgttgta atcctttcaa agtatagtaa
    14821 acgaggactt tttgctaata agttagctag tcatcgtttt aaaaccttat agcgtttgaa
    14881 acttatttaa tgttagcttt ttggataagc tcaaataagt ttttcttaga atcgttagga
    14941 actttacgga cgtcgaatgt aacacctagg tcgcgtaatt tttcaaaagt agcaacgtct
    15001 tctttgtcca ttgataaaac gttgttaacc attgttttac cagttgagtg agccattgaa
    15061 ccaacgttca attcgttgat ttcaacacca ccttcaaccg cgcgaagggc atcttgaggg
    15121 gtttggaaaa gaataagtgc gtgtgtgtta ccaaaacgag gatctttaga agcttcaatc
    15181 aatttgctaa ttggaacaac gttagctttg acaccaccag gtgcagcctg tttgatcaat
    15241 tgtttgcgaa ggtcatcttg agcaacctca tcagaagcaa caatgatacg gtcagcctta
    15301 gaagctggag tccatgctgt tgcaacttga ccgtgtaaaa gacgagtgtc aacacgagca
    15361 agattgatct ttaatttacc atctccgatg acagttccag caggaatagc tccttgaagt
    15421 gcgttaacaa ctttttcagt tgcagcagtt tcttcaactg ggttaaggtc ttcaggaagg
    15481 gctttgatac cgtccttaga ttccttaatg atgtttgcag caacttgttc aacacctgca
    15541 ccagcatcca taagacgttc agtgtaagct tgaataagca taggcaagtt gagaccagtg
    15601 atgatcgcca ttttgcgctc aggattttca cctgccacac gactagcttg gttaaatgga
    15661 gaaccactcc aaaggtcagc tagcacaagg atttcatcat cagcatcaaa ttgttggata
    15721 gcattgttga agtgtccata caaatcatct ggtccttcgt ttggcatgaa agtaacaact
    15781 tgaactttct cttgttcacc aaaaatcata gaaccagatt gatgaatacc ttcagcaaat
    15841 ttaccatggc tggcaataat aataccgata cccattcttg ttcttcctcc tttaaaatat
    15901 ttttcgttga aatgaagaag aataatctcc ttccaaaact aaattttggc aattcttttt
    15961 cacttaaacg aaattcaaga acgtttctat tttaaaacgt tttcactaaa aaatcaagcc
    16021 ctttcggaaa aatagtacaa aacatgaaaa aattttcaga atttaggatt aaataggcag
    16081 attattgata ttaaaggtgt tttcaagctg tttaaaacaa aaatatgctg aaaaatgttg
    16141 aaattattaa aatgattttc attttttgat agctgttgac agaagaagaa ccaccatttt
    16201 ctgaaaaata gtggtttcgt ttgctactac aagaaaagtt cttccaattt tttggcaaca
    16261 ccgtcttctt cgttggaaaa attaagctgc tgatcagcat aaggcagtag cacagggcta
    16321 gcatttttca tagcataact agttccagca aaagctagca tctcagtgtc attgtgttca
    16381 tcaccaaagg cgattaaatc ctttttatcc atattgtaaa ttccaaggag atagttcaga
    16441 gcgtaagctt tattgacatt cttagacgaa atttcaagaa tgttgagagg gcctccccaa
    16501 gaatcaattt caatttcatc tttgaagaaa gcacgcatgt ttttagctaa ggcatacttg
    16561 tcttcgtgat gcgtttgcat caataaggcg tttggatttc gagtgatttt ggtaatctct
    16621 aatgccatgt cctgagtaat ttcatcaaca ccaaagagtt gcgggtcgat actttctgga
    16681 ttggtcatgg tgatgtagac attttttcga tactcactgg caataaaatc catctggaaa
    16741 tcatcctgat attttagtaa ccttagtagg tagcctttgt ccaaagtgac attatgttca
    16801 taggcccatt tttgttcagg catgtgggtc aaggccccat taaaagtaat gattggggtc
    16861 tttaaattga gctgcagata gtagtccagt gccatgcggt aaggacggcc tgttgaaata
    16921 atgacgtggt gacccttgtc ttggacagct ttgatggttt tttgagtata ggtagagatg
    16981 gtgttgtcat gatgtaataa ggttccatcc aaatcaatgg caatcaattt tttagtcata
    17041 attaaattat agcaaatttt gctccaagat aaagagaaaa gataagttaa gcgtattcat
    17101 tagtaaaaaa gataggataa ctgttttttt aatgaataaa tgaaggtata gacgttgaaa
    17161 agtgaacaaa aaatggtaac atagtgttat tgttcatttt aatacgaaca ataacaataa
    17221 taatgaggtg tcaaaaagac ttatctagtg ataagcgctt tttgatttta cctactattt
    17281 aattaaggag actccatttc aatggaaaag ttttttaagt taagcgaaaa tgggacaact
    17341 gtctcaactg agattatggc tggtttaacg accttttttg ccatgtccta tattttgttt
    17401 gttaacccaa gtattttagg tgcagcgggg atgccctcta atgctgtctt tttggctacg
    17461 attatcgcag cagccatatc aaccttaatt atgggattat ttgccaatgt gccttatgcg
    17521 ctggcaccag gaatgggact taacgctttt ttcacttata cagttgtttt tgctttaggc
    17581 ttttcatggc aagaagcctt ggcaatggtt ttcatttgtg gattattcaa tatttttatt
    17641 accgtaacca agtttcgtaa aagtatcatc aaggcgattc cagttagttt acagcatgct
    17701 attggtgggg gaattggtgt ctttgtagct tatttaggat ttaaaaacgc aaatatcatt
    17761 actttttcta tctctgctga aaatatagta atggtaaatg gtgttgaacc ggctaaagca
    17821 tcggctaaaa catttgcaga tggtctatta tttgtagacg ccaatggtgg agttgtacct
    17881 acgatttcta gttttacgga ttctagtgta ttacttgcta tttttggttt acttttaacg
    17941 acagctcttg tgattcgaaa ttttagaggt gctattttaa ttggtattgt cgcaacaact
    18001 cttgtaggta ttcctttagg aatagtggat gtgtccaacc tcaattttgg gatcagccat
    18061 attggtgaag cttggactga attaggtaca actttccttg cagctttcga tggtttgagt
    18121 tctcttttta gcgattcaag tcgtttaccg ctagttttca tgactatttt tgcttttagt
    18181 ctatcagata cttttgacac aattggtacc tttatcggaa ctggtcgtcg aacaggtatt
    18241 ttctctcaag acgatgagaa tgctttggaa aatagtatag gctttagttc aaaaatggat
    18301 cgtgcgcttt ttgcagatgc tatcggtact tctattgggg ctttggttgg aacttcaaat
    18361 acgactacct atgttgaatc agcagcagga attgctgaag gtggacgtac tggactaaca
    18421 gcagtctcca ccgcagtatg cttcttatta tcaatattgc tattaccgct tgtaggtatt
    18481 gtcccagctg ctgctacggc tccagcttta attgttgtgg gtgtcatgat ggtgtcttct
    18541 tttcttgatg ttaattggag taaatttgca gatgctcttc cagctttttt tgccgctttc
    18601 tttatggcgc tgtgttactc tatttcctat ggtattgccg ctgcctttat tttctattgt
    18661 ctagtaaaag ttgttgaggg aaaaacaaaa gatattcacc ctattatttg gggagcaacc
    18721 ttcttgttca ttgtaaattt catcatatta actatcttat aaccaaataa tttacttatt
    18781 ttgctactaa ctgccgcttc tttaattgat tttaaccttt aatataaaga agcgttttta
    18841 ctttacgaat accttattgg agtcatcttc taggtagtgc ttggaagtat gttttttttc
    18901 tgatataatg ttgctatgtt ttatagtgaa aatgaatata ctcttaaagc ttacggagaa
    18961 accctaggta cttatttgtc tattggagat gtgattgtat tgtctggcga cttaggtgcc
    19021 ggtaaaacaa cattggcaaa aggtattgct aaagggatgg ggattagtca aatgatcaaa
    19081 agcccaactt atactattgt tagagaatat gaaggacgtc tacctcttta tcacttagat
    19141 atttaccgtg ttggtgatga tccagactct attgatttag atgattttct ttttggtaat
    19201 ggtgttaccg ttattgagtg gggagaatta ttaggagagg gattattaca agactatttg
    19261 caaattacca ttactaaacg agacaaaggt cgtcaactag acttgttagc gcatggtgag
    19321 cgctctcgtc agcttctgga gataatgtca catgattcag caaagtctcg ttattgaaga
    19381 aggagtacct caagatgcaa aagctgtact ttccttagtg acggcagctg ccaaagaaac
    19441 agattttata acaggcgtag agactatatt agaggctact cctcaagaat tgtcagactt
    19501 tttatcaaga agtcaaacct cttttattga tttttgcctg ctagctagat tagatgaaaa
    19561 agtagtaggg cttttaaatc tttctgggga agtattatcc caaggccaag ttgaagcaga
    19621 tgtatttatg ttagttgcta agacttaccg tggttatggt atcggtcaat tattgttaga
    19681 gattgctctt gattgggctg aagaaaaccc ttatatcgaa agccttaagt tggacgttca
    19741 agtaagaaat attaaggcta tttacctgta taaaaagtat ggttttcgta ttgaaagtat
    19801 gcgaaaaaac gacatcaaat caaaaaatgg agatgactta gatatttatc atatgcgtaa
    19861 atctgtaagt cagtaagtga atcgatgaaa attggaaaaa aaatagtttt aatgttcaca
    19921 gctattgtgt taacaactgt cttggcatta ggtgtctatc taactagtgc ttataccttc
    19981 tcaacaggag aattatcaaa gacctttaaa gatttttcga catcttcaaa gaaaagtgat
    20041 gccattaaac aaacaagagc tttttctatc ttgttgatgg gtgttgatac aggctcttca
    20101 gagcgtgcct ccaagtggga aggaaacagt gattcgatga ttttggttac ggttaatcca
    20161 aagaccaaga aaacaactat gactagttta gaacgagata ccttaaccac gttatctgga
    20221 cccaaaaata atgaaatgaa tggtgttgaa gctaagctta atgctgctta tgcagcaggt
    20281 ggcgctcaga tggctattat gaccgtgcaa gatcttttga atatcaccat tgataactat
    20341 gttcaaatta atatgcaagg ccttattgat cttgtgaatg cagttggagg gattacagtt
    20401 acaaatgagt ttgattttcc tatctcgatt gctgaaaacg aacctgaata tcaagctact
    20461 gttgcgcctg gaacacacaa aattaacggt gaacaagctt tggtttatgc tcgtatgcgt
    20521 tatgatgatc ctgagggaga ttatggtcga caaaagcgtc aacgtgaagt cattcaaaag
    20581 gtattgaaaa aaatccttgc tcttgatagc attagctctt atcggaagat tttatctgct
    20641 gtaagtagta atatgcaaac gaatatcgaa atctcttctc gcactatccc tagtctatta
    20701 ggatatcgtg acgcacttag aactattaaa acttatcaac taaaaggaga agatgccact
    20761 ttatcagatg gtggatcata ccaaattgtc acctctaatc atttgttaga aatccaaaat
    20821 cgtatccgaa cagaattagg acttcataag gttagtcaat taaaaacaaa tgctactgtt
    20881 tatgaaaatt tgtatgggtc aactaagtct cagacagtaa acaacaacta tgactcttca
    20941 ggccaggctc catcttattc tgatagtcat agctcttacg ctaattattc aagcggagta
    21001 gatactggct catcttatca gagtgctagt acagaccagg actctactgc ttcaagccat
    21061 acgccagcta cgccgtcttc ttcatcagat gctttagcag ctgatgaatc tagctcatca
    21121 gggtctggat cattagttcc tcctgctatc aaccctcaga cctaacgttc tgagggtttt
    21181 tatgtttaag gtatccgatt ttgaatataa aaaagacagg tatcaaagat gatagctgtc
    21241 tgtgatttca aataaagtat atgagggaga taatactatc tggaatggtt gttagttaaa
    21301 atgacggttt ggcaagtagg tgattcacta gaaattagct cactatcttc ttgatatttg
    21361 gccatacgat tttggatttc agtgaggtgt gcttgtgtct cttggttaaa gtagcgccat
    21421 ttatgggcca agtcctgact aatattttga attacttttc cttgactgtg aatgaggtca
    21481 aggtttgctt taatgttatc taaatcaagt tgtgcagcat cctttgcttg tcgactgatg
    21541 gataacagct cttttatggt acaacgtttt tgatatgctt tgtaagcggt aaaagataat
    21601 aatccgacgg ctatcacggt tttaactttc atgaactaac ctcctgattg atttgtcggg
    21661 cgagcttttc caagacagga aagtcaggct catgagtggt gtattgaatt gagataccgt
    21721 cttcttcatt ataacgagga acgagatgaa catgagcatg gaaaacagtt tgtcccgcta
    21781 aggcttcgtt attgttaatg atattcatgg cagtagcgcc agtagctgac tggatagcac
    21841 gagtaatttt aggaatacga gcaaagagat gactagctgt ctcagcagtc atctctagta
    21901 agttacgaac atgttgctta ggaatgacta gagtatgccc ttttgtagtt tgtgagatat
    21961 ctaaaaaggc gaggacctgc tcatcttcgt agactttcga agaggggata tccccttgaa
    22021 tgatgctgca aaaaatacaa ttttccattt tatatcacct tctctacttg aatttgttat
    22081 aatatattat aagtatatca aaaactagaa agaaattata tgttaaacat taaaaatcta
    22141 acaggaggat atcacaatat ccctgtttta aacgatgtct cattttctgt agacaatgga
    22201 gagttagtcg gtttgatcgg gctaaatggt gctggaaagt caacaactat caacgaaatt
    22261 attggctttt tgaagcctta tcaaggaagt atttctattg atggtttaac tctagcagaa
    22321 aatgcagttg cctaccgtca aaaaataggt tttatcccag aaactcctag tctctatgaa
    22381 gaattgacct tatcagagca tatcaacacc gtagctatgg cttatgatat cgacttagaa
    22441 gtggcacaaa aacgagcaca acctttcttg gaaatgtttc gtttaactga caaacttgaa
    22501 tggtttcctg tcaatttttc taaagggatg aaacaaaaag tcatgattat ttgtgccttt
    22561 gtcattgatc caagtctatt tattcttgat gaaccattct tagggttaga ccctttagct
    22621 atttcagact taatccaaac gctagaagtc gaaaaagcaa aaggaaaatc tattttgatg
    22681 agtacacatg ttttggactc tgctgaaaga atgtgtgatc gttttgtgat tttgcatcat
    22741 ggacaagttc gtgcccaagg aactcttgct gacttgcaag aagcttttgg tgaccgttca
    22801 gcgagcttaa atgatatcta tcttgcatta acaaaagagg attaagtatg aaagcattat
    22861 ttctgaaacg ccgtcaaaac tttcaaaaac aacaaaacaa ataccttcgt tacgtcttaa
    22921 atgatcactt tgttttggtg ttgatgtttt tattaggctt tgctatggtg cagtatggtc
    22981 agctactaaa ccattttcca acgaatcatc tacctattca ggtttgtctt gggatattga
    23041 tcccattatt gctcagtatg ggatctatag caacgtatct agaagaggca gatcagcatt
    23101 tcttacttcc aaaagaagaa gaagtgattt cttatatcaa acaagctgag cgactctctt
    23161 ttttgctttg gggaactttg cagactgctg tattgctctt tttatatcct atttttagac
    23221 gtctaggttt gtctttattt atttttatta tcttagtttt gatattattg gcactaaaaa
    23281 gagtagtgct tagtcgaaag acaagatatt ttttgagagg aaatcgttta gattgggcaa
    23341 aagctgtcgc atttgaaagc aatcgcaagc aaagtatttt aaagttttat tctcttttta
    23401 caacagtcaa aggaatttct acaaaagtga aagaaagaac ttatctaaat cctcttttaa
    23461 aattagttaa gcaaactcct tcaaaccttt ggctgtccct ttatgctaga gcctttttac
    23521 gtagttcaga ttatttgggc ttatttttac gactaatgct attaagctca ttatctgtat
    23581 tttttattca taatctttat ttgtcagtaa gtttagcact catttttaac tatttagttg
    23641 tattccaatt actttcactt tattatcact acgattacca ttatatgact agtctttatc
    23701 ctgaaaatag tcggagcaag aagaaaaatg tgttaagttt tttacgaggt ttaagctttt
    23761 taatgttaat cgtcaatatg ctgtgttgct catcagcgcc aaaagcactc attttgattg
    23821 taggtatggt tttcatagct tgtatatatc taccttataa gttaaagaag atcattgact
    23881 aagtagccta aaacaagtaa aataggggta aacgactata aaagatagaa aaggagggcg
    23941 ataaggtgac aacgacggaa caagaactta ccttgactcc cttacgtggg aaaagtggca
    24001 aagcttataa aggcacttat ccaaatgggg aatgtgtctt tataaaatta aatacgaccc
    24061 ctattctacc tgccttagca aaagaacaga ttgcgccaca gttactttgg gccaaacgca
    24121 tgggcaatgg tgatatgatg agtgcccaag aatggcttaa cggccgtaca ttgaccaaag
    24181 aagatatgaa cagtaagcaa atcattcata ttctattgcg ccttcacaaa tctaaaaaat
    24241 tagtcaatca actgcttcag ctcaattata agattgaaaa cccatacgat ttattggttg
    24301 attttgagca aaatgcaccc ttgcaaattc agcaaaattc atatttacaa gctatcgtta
    24361 aagaattaaa acggagctta ccagagttca aatcagaagt agcaacgatt gtgcatggag
    24421 atattaaaca tagcaattgg gtgattacta ctagtggtat gattttttta gtagattggg
    24481 attctgttcg tctgactgat cggatgtatg atgttgctta cctgttgagc cactatattc
    24541 cacggtctcg ttggtcagaa tggctgtctt attatggcta taaaaataat gacaaggtta
    24601 tgcaaaaaat tatttggtat ggtcaatttt ctcacctgac acaaattctc aagtgttttg
    24661 acaagcgtga catggagcat gtgaatcagg agatttatgc cctcagaaaa tttagagaaa
    24721 tatttagaaa gaaataatgc gagttagaaa acgaaaaggt gctgaggagc acttagctaa
    24781 taacccacac tatgtgatat taaatccaga agatgccaag ggccgttggc atgacgtttt
    24841 tggaaatgac cgtcctattc atatcgaagt tggttcagga aagggcggat ttatcacagg
    24901 gatggccttg aaaaatcctg atattaacta cattggcatt gatattcaat tatctgtcct
    24961 aagctacgcc ttggataagg tattagcttc agaagtaccc aatgtcaaac ttctgcgagt
    25021 ggatggatca agtttaacca actattttga agatggtgag gtggatatga tgtatctcaa
    25081 cttttcagat ccttggccaa aaaccaagca tgaaaaacgt cgtttgactt acaaggattt
    25141 cttagacact tacaagcgta tcttgccaga acacggtgaa attcatttta aaaccgataa
    25201 tcggggcttg tttgagtaca gtttagctag tttttctcaa tatggcatga ccttgagaca
    25261 gatctggctt gacttacacg ctagtaacta tgaaggaaat gtcatgacag aatatgaaga
    25321 gaagttctct aataaaggtc aagtcattta ccgagttgag gcaaatttct agtagtattt
    25381 tgtttcaaaa tatgataaca taaaggagtt gatacagtat taacttcttt agtaatggag
    25441 aggtcgcata gtggcttagt gcgcacgctt ggaaagcgtg tagtccttaa cggggctcgg
    25501 gggttcgaat cccctcctct ccttttatcc tcttgatata tcaagggttt cggggtcttc
    25561 gcccgtcttt cacccgtcaa atttgagatt ttgaaaaatg tttttgacct gatggtcatt
    25621 tttttctttt aaactttcta tctgatgagc gtaaactttg agtgtgatgt tgagattttc
    25681 gtgccccaac agcttggata ctgatattaa atcaacgccc tgcgctatca aataagaagc
    25741 gtaagtatgc cttagcgagt gattggttag atttctgcca gtcactcttt ttataacttt
    25801 gttcgttgca ttatttgacg ctccaaagca tacacgaccg agtttattat cctgataata
    25861 gtcgctctta aaatttctta gtatctcaag gctattttta tcaatcggta ccttcctgat
    25921 ggactgctcg tttttggtcg gaccgaaatt ttgagaaatt gaataatcaa aagttttatt
    25981 aatatcgata tagccattct caaaatcaat gtctttccaa gtcaaccctt gaacctctgc
    26041 aaatcgtaaa ccggtgacgg ctatgatata cgttgtcagg tatgatgggt acttgacttt
    26101 gcttttggca accttgataa aggcgaggta ttctgattct tccatgaatt tctctgactc
    26161 ttctttagcg ggtttttgcg atctaacaat ggccccatcg gcaaaattat ccctaataat
    26221 gccatcacga atggccattt tacaagcacc cttgatttga taatgaaatt tatccagagt
    26281 ttgttgggct acttttgcgc caaagtcatt aaggacttgc tgataaaagg tgtgtgtgat
    26341 actcttgagc ttccgagtcc caaaataatg ttcgatgtgc ttaaaatttt tggtgtatgt
    26401 ctgccatgtc tttgcggtaa tgtgaggcct tttatatatg tctgcccaac gtctgttgta
    26461 gtctaaaaca gtaatatcta aaaggtctgc ggtcagcgta tttaaatcaa tttctgcttg
    26521 ctgagcagcg acttttgcaa gtgtttttgt tttaaatcca cgttttgatt tttctctttt
    26581 tttaccttga ctgtcacgat aggtgatgcg atactcccac ccagaaccta attttcgata
    26641 aaatgccatt gttttcacct catttttctg ttaaaatggg tacaagaaaa cgaccttttt
    26701 aatggttgtt tcttatacgg ttttgcctca cgctctcctc gccaaatttg agcgtggggc
    26761 tttttttatt taaagaaatt attaacactt acatactgag cctgcgtaag cttagttttt
    26821 aaggttttaa cttctccggt ttgtatattt cttaaggcta ccgttgcttt tccaggtatt
    26881 tcttcagaag tggttgtggc agtagtgtta atggttcctt ttttctttct agccccacca
    26941 gccattccgc cgataatggt tccaacgcct ggtgccaaaa ctttacctat tgctgcccct
    27001 cctagaacac tactagaacg ccctttttga gtagttttcc cttttgttac tgttttttca
    27061 ataattcgtg acccatcaaa ttgaaggcta acaaactcat aaagctcagg agtttcagaa
    27121 taaaaaccga tataatattt tccatctatg gtttttcgta tagccgtttg tgctcctagt
    27181 tctaaattta gggcaggggg taggattatt ttgctctctt gtgaggcttt ttttcttagt
    27241 tcgttagctt taattgcacc gtctttagct ttccctgcag ctgttatggc aaattctttt
    27301 aacttagcta tatccatgct tgtctcctta actaattaaa aactctaatt cttctttgat
    27361 catcacttcg tcagcgattg aggtcagttc atacttttcc atgaattgga tgtagttgaa
    27421 atcctccata ttatcccaat aggatagctc ttcttttaag agatggtgta tcatacatct
    27481 atctgcttgt agctctgctt tttctttgtt tagtttgtaa taactcgcag tatgttctct
    27541 atgtcctagt tcatgataga tgactttgtg tttatatata ccgtcaagat aggtgtcaat
    27601 cgcaatgacg ttgtgttttt tattaaacat tccaggggtg tttgtacccc taccgtcaaa
    27661 gtacactaaa tcgattcctt tttcctgaca gactgtttct ggtgtcatca tagagcagtc
    27721 ctctctattt tctatttttg atgcgcgttt ccaatataga tgtgataagg tctatatcct
    27781 cgtcgttgag ttcgtgaccg tcgaagaaga aagtctcttc tgcatctttt tttagatcaa
    27841 ttgcgaccga agcgtgccca tcttgcgcaa tcttaggatt atcagttcga cctaataggt
    27901 aatcggttga tacgtgaaag tagtcagcga ttagttgtag tctttctgct gaaggctttt
    27961 tatttttaat tgtatagatt gaatttctac tcaatcccaa tttttcctca agcaatccca
    28021 aggaaatacc tcttgatttt gcaagttctt taattctttc gaatgttgaa aacattgatt
    28081 tgtcagcctt tctatgagat gacaaaaaat atctataaaa aagcatgtaa actacttgac
    28141 tgattacaaa ctttagtcta gaatataatt tgtaaagcga atataaaagc gaaacaaaaa
    28201 acgaaaatta aaactaaaaa aatataactt tggcgagttt ataagtagtt taataatgtt
    28261 tttgttggtg tttttactat accttgattt tagaatatag tttgtaaaat gtcaatacta
    28321 aagttgaaaa acttcgcgat tattttcgct ttacttttaa taaaaaaact cccgattggg
    28381 agtagaaagg agagagtagt atggatgaaa aaaatccact cgaacagttt gaagagtttt
    28441 gcaagaataa ttcatttgag tatgttaaag gctgggttga tggcatgcaa tcagttttaa
    28501 atattctctc aatggaatta tctaaaaaaa atggatctac ttcggaggtt gattgaatac
    28561 atcgttttct tctaaatatt tagagagcgc tggtgtcatt gctgagccta acatggtcat
    28621 tagtccttga gcaacagtat tgtttacttc tgcttctgac tgcttttgaa tattttctaa
    28681 ctctaaagtg tgagcttctt tcattttctc aagttctcga aaatgatttt tttccatagc
    28741 ttcaaaatca tgttgatact gtttttcaag cctttctagg tcattactat gatttttttc
    28801 gatagtagtc atctcatgat tgaacttttt ttctaacgat tcaatatcag atctgttctt
    28861 atatcttgaa gctaagaagc taataatagc agaaattgca gctggcaaaa cagctgttgc
    28921 aatagttaac cagaaattaa atttttccat aataaacctc aacattttta tttatattat
    28981 atcagaaagg agagcagatg agtcaacaac acaaaaaatg gtcagctcta gttgaacagc
    29041 gattaaaaga aaaaggttgg acaagagtcg acttggcact tgcggctggt ttgcggagtt
    29101 caggaacgat tactgactta cttactaaag gaaaaggaag cgttgacctt aaggttaggg
    29161 tttcaaaaat tcttagtatc cgtgagccgt gggagttgtt cgaaaatgaa tagtacacaa
    29221 aaaagccact gctggaacag tgacttagct aaaacatatt aaaggaattt tatcatggat
    29281 agcagattat tacaaatgct agatgagttt gaagcgggcc ttattgatcg caaaatcaaa
    29341 gtcatgaaaa tgattaacaa tgagacggaa atttatccgc tagaactcaa taaaaagcaa
    29401 gtcagtaaga tgtttggggt tgaccctaaa acatttgacg caagatttaa cagccacaaa
    29461 gatttcccgc gtattgaaac ggggggtcgg gaaaaatacc cacgagattt agccatcgag
    29521 tggtatcaca gaaattggag tataacatga acaaattaga actattttta ttagcaacaa
    29581 ccgtcatctt agcggtcatt gctagggtgc aacacgaagt catcaaaaaa cacaattcgc
    29641 cagagaataa acgcagaatt tttagggaag tggctttaga aaacagcaag aaatggagtg
    29701 cagaacgtta tgtctaaatg ggaattacaa aagcaactgt tggctaatgc gacagatttt
    29761 gagagtacag tagaagctct gtttgagtta aatgaattgg gtgaaatctc ggacgaggag
    29821 ctcttgttaa atttgagaag tagctacagg aaatactcag aggaaaaact ttgtattatt
    29881 gacaaactca ttaagcttgg aggcgcagaa tgatgacaac ttttgaaaag attatcaaga
    29941 accttaatcg gattgagagg gaattagtta agcccgaagc tgaaagacga gatagcttac
    30001 ctatcctatc tgctcagttg agtgctatca agcaagattt aaaattgatg ctttggattg
    30061 aacttccggc gttaaataac gtagaaaaat atgaagctct ttatcaagga aatgatttta
    30121 aattcagtga aactttctta gagcgtcaag cgacgagaaa agccttttgg agacgattag
    30181 ctaaagaaac tttcaaagaa gaccaggata agaggaactt tgttaagttg gctgagaaag
    30241 aatttaaagg tgcaactttg tcatggcaag aatttttgtg gggtaaaaag aaatgaacat
    30301 atcactaaaa tttattgatg aaaccctagc tggtttaaat gatattttac gccagggggg
    30361 gctctcttgt tcccaaagcc aagccctggc ggacgcagtt tttatattaa cagctttaaa
    30421 acaagtaatt gaggagagaa aatgaactat ttagaatatg cactagctta tcttgaacgt
    30481 gagctagaaa ttattgacga cgaagttatc gaagttgagt tgccgggcgg agattgggag
    30541 tttgtgccta acccctacta cgaggaaggt ttacatgata gcccacacta tcgtagtcag
    30601 gttgctaaag acattcttga tattaaagga cttttgggga ggtgaggcta ttgattgcat
    30661 caaaaaagac ggcttaggaa accgtcaaca attatacaca attaaattat aacacagaaa
    30721 gagagaaaca caatgattga agtaactttt aaagcagaga gcatggcagg tgttttcgat
    30781 gctatgcgtg aagctctgga tatgccgaag acagtaacca aggatgttgt tgaagaaaaa
    30841 ccagctccta aaaagaaaga agagtctgta actttgacat tagctgatat taagaaaatg
    30901 gctaaagcca aggttgaaga agggaagtca aaggacatca aggaagtctt gaaagagatg
    30961 aacgtcgcaa aagttggtga acttgaagaa taccagtttg ctgagtttgt tgagaagttg
    31021 gaggccctct aatgccagta gaaaatcacg cactactgtc tgcttctagt gctcacagat
    31081 ggctttattg ccctatgctg ccgagattag aagcagacta ccctagccgc gataccgtat
    31141 acactcaaga gggcacaagc gcccatgagc tttctgaaat caaactgatg tacaagtctg
    31201 gtaaaatcac caagcgtaaa tttaacacgt taaccaaggc ttttaaggaa aactcagact
    31261 tctacaacga agagatggaa gagatgacgg aactttacac agatattgtt atggagcacc
    31321 taaatgctta tgaggatgcc gaaattgaac ttgaaaaacg gattgatttt agcgactggg
    31381 tgcctggcgg ttttgggact tcggatgtcg tcattttggc ggatggggtc attgaaatta
    31441 ttgatcttaa gtatggcaag ggtatgcctg tgtctgctaa ccaaaacccg cagatggggt
    31501 tgtacgctct gggagcttac gcttcctacg atatggttta tgactttgac cgtatcaaga
    31561 tgacaattat tcagccgcgt ttagattcgg ttagttctgt tgacatttac gtagaggagc
    31621 ttctgtattg ggcggataat gttgtcttgc ctatggccgc tcaagcagac gcaggtattg
    31681 gcgactggaa cctaagtgaa aaagtattgc agtggtctcc tgtcgcagct aaattggtgc
    31741 caagagcgca agaaaactgg gaattaattg ataaatatga ctatcaggag cctgtctatt
    31801 tatctgatga agccgtcgca gagatccttg acaaagcctc agctatcaag aagtgggttg
    31861 agtcagttga agcctatgcc ctgaaagaag cactctcagg aaaagaagtt ccaggctata
    31921 agattgtcga aggtagaagt aatcgtgtgc ttactaataa ggaggcagct gcgacaatac
    31981 ttgagaaaaa cggctttgaa gatatctaca aaccaaaaga gctactgtcc atgggggctc
    32041 tagagaagat ggtcggtaag aagcgttttg aggacatgat ggcctttatc cttgataaac
    32101 cacaaggtaa acctgtcctt gtcaaaaata gcgacaagcg acccgcgtta aatagcttag
    32161 aacaagcaat aaaggagttt gaatagaata tgaagattta taaagtaaaa gttaagttta
    32221 gacaaacatg ccataaaaaa ttcaagggta agaaatacag ctactttagt tttgaagagt
    32281 tgcgggtagg tgaccttgtg gttgtcgaaa ctgtttatgg ccctagcgta gccaaggtaa
    32341 cagaagttgt tgatgccaat gagcttttta cagctaccag ctatgttatt tccaaagtgg
    32401 atacttcact tttagcgggc aagaaagagt taatggctac cgccttgact gtaaaagcaa
    32461 atattgatgc ggaaacggct gagtttgcag cgaaatataa agatgcttat tatttaggcc
    32521 tgtttgatca gtacaaaaac caaaatccag aattagcaga gttattaaca caattaaaag
    32581 aattatagga gaagaattat gacaccaagc acaactaaag tagtaaccgg aaaagtacgc
    32641 ctaagctatg cagctttact agagcctaaa gcctttgaag gtcaagaggc taagtattca
    32701 acagttatct taattccaaa aacagacaaa gtcacaatca agaaaattaa agacgcgcag
    32761 aaagctgctt atgaagctgc caaggacaat aaactcaaag gggttaaatg ggagcgcgtt
    32821 aagacaacgc ttcgtgacgg tgacgaagag atggatactg aagagcaccc agagtacgct
    32881 ggacacatgt tcatgtcagt gtcaagcaaa actaggccgc agattatcga ccgttacaaa
    32941 aaccccgttg actccgcaga cgaagtttac tcaggagtat acgcacgagt atcattaaat
    33001 gcctatgcct acaatactgc gggtaacaag ggtatctctt gtggtctgaa taacgttcag
    33061 attgttgcta aaggagacta ccttggcggt cgttcgtcag ctgatgcaga ctttgacgag
    33121 tggaacgaag aagaggacga agacgacatt ttatagtaga gggcctcttt agaggctcct
    33181 catttttaaa ggagaggtat gagacattta aatattgata ttgaaaccta tagttcgaat
    33241 gacatcaaaa atggggttta caagtacgct gacgctgaag attttgagat tttacttttc
    33301 gcttactcta tagatggcgg agaagtagag tgccttgatt tgacaaggca gtctctacct
    33361 gaagacatca aagatatgtt atttgatgat aaagtccgaa agcacgcctt taatgcccaa
    33421 ttcgaaagag tttgtctcag tcgttacctc ggcctacctt actatttaga tcccgcacaa
    33481 tggcagtgta cgatggtact tgcacaagag ctgggtctgc ctgcaagcct ggaaaaatgt
    33541 gcgctgtatt taaaattagc gcaagaaaaa gacgcagcag gtaaaaatct cattaaatat
    33601 ttttcggtac cctgcaagcc tacaaaagcc aacgggggaa gaacgaggaa cctgcccgag
    33661 catgatcctg gaaaatggca aatgtttatt aactactgta tgcaagacgt tgttgttgag
    33721 atggcaattg ccgaaaaact agagtcagtt cctgtgcacg accgtgagtg ggattactac
    33781 gtctgcgacc agagaatcaa cgacagaggc gtggcacttg ataaagagtt agttgcttcg
    33841 gctttgtatt gtaaagatgt taagatggaa agtttgtctg gtgaactaaa ggctctaaca
    33901 ggacttgata atcctaatag cagggcgcag ctgctaccgt ggctaaaaga acacggttat
    33961 tcggctaatg ggctgactaa agctgatgtt gaacaggaac ttaagaaggc cgaaggagag
    34021 cttaagagag ttttagaact taaactacaa accgcgatgt caagtctaaa aaaatatgaa
    34081 gctatggaaa gagctatgtg ctcagacgga cgagttcatg ggctacttca gttttacggg
    34141 gctagccgga caggaagatg ggcgggcaga gttgtccaag tacagaactt agctaggaat
    34201 tatataaagg atctagatga tgctagagag tatgttaaaa agcgtgatat tgatgctgtg
    34261 gagattttat acgatagcct taacgacact ttaaagcagc tcgtgcgaac ggcactcgtg
    34321 gctaaagatg gctatacctt ctatgtctct gacttctcgg cgattgaggc tagggtgatt
    34381 gcatggtttg ctggagagca gtggaggctt gacgtgtttt ctacgcacgg gaaaatctac
    34441 gaggcgtctg ccagtcagat gttcggaatt ccaattgagg agattgacaa ggaactacgc
    34501 caaaagggca aaatttcaga gttagcactt ggctatcaag gaggtcctgg agcgcttaag
    34561 cagatggggg ctctaaatat gggagtcaag gaagaggagc ttcaagggtt agttgatgac
    34621 tggcgcaggg ccaataagaa aatcgtccaa ttttggaaag acgtacaaag agccgccatc
    34681 aaagccatca aatcgagagc accgataaaa cttggaaaaa tacgatttag ataccggaaa
    34741 ggtttcctct ttataacatt gcctagcggt aggaacttag cttatgcaag agccaaggtt
    34801 gagccaggcg actatggaga caaaatcatc tatgagggcc aaggagatag ggcctatttc
    34861 acagcgcaag agacgtacgg cggtaagctt gtcgaaaata tcgttcaggc gacggccagg
    34921 gatattctag ctgaagcgct tctgaggatt gaagctgcag gctatgatgt tgttttccac
    34981 gttcatgatg aggctattat cgaaggttca ggcctgataa tcgaagaggt taatgatttg
    35041 atggctcagg ctcctgaatg ggcggagggt cttcctttaa atagcgaagg ctatgtaaca
    35101 aagtattata tgaaggatta gatagatgaa gcaagaaaaa ctaatagtaa agtcttctcc
    35161 cctgcaagag cttcatatcg caacaggtag ttcgcgaaca gctaagacat ggaaaaatat
    35221 cacgctaact tggcaggagc tggttgagag gttagagaaa cctacagtca cccaagagac
    35281 gtttgcggag taccagaaga tgtctcgagc agaaaaaggg caagcaaaag acgtaggggg
    35341 ctttgtcggt ggatggctaa agcaaggtaa acggaaaaat gaaaacgttc aaagtaggtc
    35401 cttggttgcg cttgacgcag atagcccaag taaagatttc ttagataggc tagacctgct
    35461 tgcagattat gcattcgtac tctacagtac tcacagccac tcaaaaaaag ctgctaagta
    35521 ccgtcttatt atccctactg accgtttaat gatgcctgat gaatatgagc cagtcgctag
    35581 atatttggct aaccaactag gcatgtcaaa ctttgatgac acgacttatc aaagcgtacg
    35641 cttgatgttc tggccgagcc actcaagaga tgccgacttt acgtttaaat ataacgacga
    35701 ggcttttctg agtgttgatg aggtgcttga cacatacccg gactggcatg actcaagctt
    35761 ctggccagaa agcccgacgc acgctgttaa aagacagcgt gaagctaaaa aacaaggtga
    35821 cccgcttagt aaaaaaggac ttattggagc cttttgtcgt aactatgaca ttagacaggc
    35881 cattgcaaca ttcttatctg aggtttatga agaaggaacg actcctgata ggtacaccta
    35941 cactgatggc tcaaccgcaa acggcttagt tatctatgat gatgtcttcg cttatagcca
    36001 tcacgggaca gatcccgtgg gggatacgct tgtaaatgcc tatgaccttg ttcgtatcca
    36061 taaattcgga gagcaagata gcgaggctaa agataatact cctactaata agttgccgtc
    36121 aagtaaagcg atgaatgctt ttgtctctga cttacccgaa attaaagacc atttaatggc
    36181 ggaggcttta ggcgatttcg atgaagagtt accagtcgaa gatgacagaa gctggcttga
    36241 aattgatgag aggggcgaac cggaggtcaa tagttatttg ctagcaacgc agattattaa
    36301 ggaggttccg atttattggg atggcttaga atttttacgc tacgacgcta aaaaaggcat
    36361 ctggttgcca aacgctgagg aatatttgaa gagttatatc tcaactaaga aactcggtaa
    36421 aattactaag attaggcaca ttagcgaaac cgtcgtagcg attaaagcac aggctttttc
    36481 aagtgaagtg tttaccgaga gtgatcttaa caagatagtg ctagcgaatg gagtctatga
    36541 cctgagggat aacaacttta agactaagtt tgatccagaa ttgcatgcca ggtcaagcca
    36601 tcccgttgtc tatgaccccg aggcgacctg tgaaaccttt gagggttttc ttagagagac
    36661 cgtcggagct gaaaatatag atttcatctt tgagtggttt ggctataact tttatcgtga
    36721 atacactatt caaaaaatgc tattcatcta tggtagcggg ggcactggca aatcaacact
    36781 aattaatatt ttacgtgaaa tgataggcgc tgataattat tcagccgtga cactgcagta
    36841 cctgatgcaa gaacgctttg caaaaatcgg cttatatcgt aagacagcta acttcgatac
    36901 cgatgctaaa cctcaatact tagcagatgg cgcagcactt aaaatgttga cgggggagga
    36961 tactattcac gcagaccgta agaataaaga gccgattaac ttttacaatt atgctaagct
    37021 gtcttttgcc atgaatgagc tcccacctat gcgagatttc agcggaggac ttaaacgccg
    37081 catgatgatc ctcgagatgg ataaggtttt aacgcaggaa gttaaggcga aatacccgct
    37141 agataagatt atgagcgagg tgcctggtat ctttaacaga gcgatggagg ggcttagaaa
    37201 ggccttaagt aagagagatt tcagtattag tgccagcatg aggtcaagtg tcgagaaatg
    37261 ggaaaaaggc aacgatgtcg tagctatgtt ccttgaagac gagtgtgaac ttggcgaaga
    37321 cttcaaagtt cctgttaggg atgtctaccc agcttataaa ttctattgtc aggattcagg
    37381 ctacaaacct ttggcaaaaa atgcatttaa ccatcggcta agagagctaa attatgaaaa
    37441 taaaaacgtt aaatctgatg gaaagcaagc caaaaattgg gtcggcttta ggttaaaaag
    37501 tgagttttag gggttacttt tttggaatta gagtttggta accctagaaa aaagttacca
    37561 agttaccggt tttttcaaac tttaaaaata aaaaatttaa aaataatttt taaaaaacca
    37621 aaaccgaatt ggaaaaatcg gtaacttagt aactcgaccc ttagaaatgg gttcgaaaag
    37681 taaccccggt agctttttgg tagccctaag aggtctttca tagcaatagt ttagcccctg
    37741 tggttaccaa tctaccactt ttcttctatt aacttaaata taaatataaa aaataaatat
    37801 atataaaata aggcatatat aaaacacata tataaaaaag agaaagtaag ttgaaaaaaa
    37861 cggtaaccct gacctttatt gaccaaagga ggaaggatga ggactgaaaa agatattgaa
    37921 aattatttga aaaagaaaac aaaagggctg tgtttaaaat ttactagtcc agggacgata
    37981 ggagtgcctg acagaattgt tgtcatgaat acgggaacct tttttgtaga ggtcaaagcg
    38041 cctggtaaaa aaccaagacc cagccaagtt gcaatgcaca aaaaaataaa agaggctggg
    38101 cagcatgttt gggttgttga ctcctacgaa tcagtggaca tggccttaaa agaaatggag
    38161 aactgggtgt gagactgcac gagtatcagg aatacgctaa gacatggata gtagagcacc
    38221 cttattgcgg ccttttactt gacatgggcc ttggtaaaac gctgacaaca ctatcggcga
    38281 tagatgagat tcaaaatatt ttttccgagg atcataagat tttaatcgta gcccctaaaa
    38341 aagtggcgga agaaacgtgg cctacggaga ttgagaaatg gcattttgat ttcacctact
    38401 ctaaagtttt ggggagcgag ggaaaacgaa ttgaggcgtt agaaacagaa gctgatatct
    38461 atttgattaa tcgtgagaat gttacctggc ttgttgaata ctacaagact aaatggccgt
    38521 ttacctttgt ggttattgat gagctgtcaa gctttaagtc tagtaagtca aaacggttta
    38581 gggctttgcg aaaagttaga ccgaaagtcc aacgccttgt aggactaaca ggaaccccag
    38641 cgcctaacag tttgattgat ttgtggcagc agatttatct gatggacaga ggcgacaggc
    38701 ttgagactag ccagactcga tttaaagaca agtattttgt tcctgataag cgtaatggtc
    38761 caatcattta cagttgggcg cttagggatg gcgcagaagc agaaatctat aacaagattg
    38821 aggacatctg tgtcagcatg aaagctaaag actatctcaa gttaccgccg cgaaccaaca
    38881 acgttgtatc agttaagtta tctaatatga aagcttacaa acagcttgaa gctgatttgg
    38941 tgttggagtt taaggataag gaaatatctg cggctaactc tgcggtcttg gccaataaat
    39001 tacttcaaat ggctaatggt gctatctatg atgatgataa atcaacagtt gctatacacg
    39061 atgacaaact tgacgcgctt gaggacatag tagaagaaag ccaaggccag ccaatcttag
    39121 ttttttatca gtatcaacat gattttgaaa gacttaagaa acgattccct caggctgaag
    39181 agttgacgtc agttgacaag tggaattccg ggaaaatacc aattcttctg tgtcaccctc
    39241 aatcagctgg gcatgggctt aatctacaaa aaggcgggca tattattgtt tggtttgggc
    39301 taacgtggag tcttgaatat tatcagcaag ctaatgccag attagataga caagggcaga
    39361 cagaacccgt tattgtgcac cacattgttg cagaaaatac agttgatgaa aaagtactta
    39421 ggattttaca aggcaaagaa aaaaatcaga acgccttact cgaagcagtt aaggcgcagt
    39481 taggagtcta gatgaaaaaa gaatatgtcg ttagaatcta cacgggtaga gaaaagaatt
    39541 ttgaggcaaa acctcagttt gaagagaaaa cctttacgcg aaaagcggac atgttgaagt
    39601 tttggaattc ttgcgaagca acagttaaag aaaaatatac tagggagtgg aaaaaatgac
    39661 agaagaacta ataaaaccgc tgctagaaga gataaagcgg aaagatgata gattgtatat
    39721 gcagtctaat gtaattgccg tcttaatgac agcagttatt atgttactga tgataagtat
    39781 agccttacaa aaccactatg agccgcaaat tacaggatta cggacacagc taagcaggac
    39841 acaaaagcag cttaaacgtg cgagtgagga tagagctaga cagacaaaac ggattgcgga
    39901 attgacaggg aatggggggt aagaaaacac atggaaataa aatgcggaaa ttgcttagaa
    39961 ttactagcgg agttaactga tggattgatc gacatggtga taacggaccc accatacaac
    40021 atatcagtaa aaaataactt tgctaccatg gggcgaacag gtatagattt tggtgattgg
    40081 gataaaggtt ttgatttact gagttggatt gacattgcaa gtcaaaaatt aactaaaaac
    40141 ggtggcatga taatcttcaa cgattggaaa aatttaggag atattgcaag acattgtgaa
    40201 aaaaatgggt tagtagtaaa ggatattttt aggtgggtaa aagataaccc tatgcccaga
    40261 aacagagata gaaggtatat tacagatagc gagtacgggg tatgggtcgt ccgaaaaaaa
    40321 tccaagtggg tatttaatcg taaatctgaa aaatacgata ggccagaata cagatatcca
    40381 gttgtggcag gagcagaaaa aacacagcat ccgacacaaa agccagtagc tctgatgaag
    40441 gacatcatta cgcgacacac aacaaaaggg gctgttgtcc tagatccttt tatgggcagc
    40501 ggatcaacag gagtagcttg tctactgacg gggagagact ttattggata tgagctaaat
    40561 aaagattact ttaatatcgc aaacaaacgg attgcagaat tgacaggaaa tgggggggta
    40621 ggtatgaata taatccatag aatggaaaac gggatagatg ctgtaggtga tttaacaact
    40681 tatgcagtac catgttgtcc agagtgtaaa aatgaaacat atagtcagaa acactgtcct
    40741 ttttgcggtg aaaatcttat ggaggtagca aatgaaaatt gaattttttt taaagcaagg
    40801 tcctactaag agcgtttcta attatggtga aatttgtatt gatgaagtaa ttggcgcaat
    40861 gaaaaataac gatgtcattg ctctaaatgg aaaaaacaac gcagttatta tacccaaaca
    40921 aaatatttct cacgtaatta ttcataaaga ggtaacggga tgagtagatt atctaatgca
    40981 cagctaaagg cttttgatga atggttgttt gattatcgtt ttattgataa caagattgca
    41041 cttcgaaaac ttgagctaca aactgatatg aatacagaag tagataaaaa tatcggtgga
    41101 ggaagagcaa attttgtttc aaaagttact gaggatgtgg ttgcaaggtg ggattcagat
    41161 agggaactaa agggtctatc caatttcaaa gaagcagtta ttgcaacgct aaatcttttg
    41221 gatgaagagc taactgatat ttttaatctt aggtggggtg ttggttcaag taacacttgg
    41281 gaagaaattg cctatacaag acatctgtca cgtaagtcta tttaccgcaa aagagagcgg
    41341 attctagaaa tattctccaa aaaaatagga atgtgacaca aaaatgtatg gaattgtcat
    41401 ctttttgatg ataaattgat agtatgagtt taaaggtaag gcaaatagtg tcttgccttt
    41461 ttgttatgct tggaggtgat ggaaaattga gtaaattaac cctaaaacag aagcgttttg
    41521 cagatgagta catcatctca gctaacgcga cagcagctgc tattaaagca gggtatagta
    41581 aaaagacagc aaggtcaata ggtcaagaaa acttgaccaa acctgacatt aaagcttata
    41641 tagacgagcg gcttgaaaaa cttgaatctg aaaagattgc tacgcaagaa gaagtcctac
    41701 aatatctcac ttcaattatg cgaggagacc aacaagaaaa gacgctcatt agcgttggag
    41761 agtttggcca gaagatagtt gacatcgatg ttggtgcaaa agatagaatc aaggcagctg
    41821 agctactagg taagcggtac agattattca ctgacaaggt tgaaatggat gtcagctcag
    41881 atgtaaccat taacgtaggt gagtgggatg acgattaaac agcgccctaa aattaatatc
    41941 gtaatcaagt atcctagcaa agtttttaac aaacatatct acgacaaact ttacaactat
    42001 agcaacttca cggaagttca ttatggtggg gcgtctagcg gaaagtctca cggtgttttt
    42061 caaaagataa ttttaaaagc acttaaccct aaattcaaac atcccagaaa gatattagtt
    42121 ctcagaaaag tcggtgcaac cgttagagac tctgtatttg ctgatatcat gtctaatttg
    42181 tcgtatttcg gcatcttgga taaatgtaag ataaatatgt cggcttttag aataacgctc
    42241 ccgaatggct cggaattcat ttttaagggt atggataacc ctgagaagat taagtcaatt
    42301 aaggggatat ctgatgtcgt catggaagaa gctagtgagt ttacgcttga tgattatacg
    42361 cagttgactt tgcgtttaag agataagaaa catctagaga agcaaatata cttgatgttt
    42421 aaccctgtgt caaaagtaaa ctgggtttat aaagccttct tcgttaagac acctaagaat
    42481 acagttgtct atcaaacaac ttataaagac aaccgcttct tagatgatgt cacgagagaa
    42541 aatattgagg aactagccga taggaatgaa gcttactaca aaatatatgc tcttggccag
    42601 tttgctacac ttgataaact aatttttccc aaatatgaca agcaaatatt aaacaaagac
    42661 aagttatcac acttgtcttc tttttttggt ttggactatg ggtttatcaa tgacccttcg
    42721 gcatttttgc atgttaaaat cgatgacgca aacaagaagt tatacgtcat agaggaatat
    42781 gtcagaaaga atttgaccaa tgacaaaata gcaaatgcta taaaggacct cggctatgcc
    42841 aaagaagaaa tcagaggaga ttcggctgaa aagaaatcta accaagagct gaggaattta
    42901 ggtattccta gaatgattga tgttacaaaa gggcctggaa ccgttatgca aggaattcag
    42961 tacctgcttc agtatgattg ggttgttgat gaaaggtgtg tcaagactat tgaagaactc
    43021 gaaaattaca cttggaagaa agacaagaaa actaatgagt atatcaacga accagttgac
    43081 agttataacc actgcattga cgccataaga tatgccgtac aagacagaat ataccagtcg
    43141 gcggatagaa gtaagcgcat gaaaaatgct aaatattatt tttaggaatg aggtgagact
    43201 ttggaagaaa aacaattttt agcaggaact cgttttaatg agaatgctaa taagcaattt
    43261 attatgctgc aagaagactt tgaagcaatt gactatgaat caaaactttg gatagatcaa
    43321 cttaagaatt acatttcaag gtttaaagca gaacaattag agcgtttgaa agagttaaag
    43381 cgatactatc ttggggataa taacatcaag tatagacctg ctaaaacaga taaatacgca
    43441 gcggataatc gtattgcaag tgattttgct aaatatatta cggtatttga gcaaggttat
    43501 atgcttggtg ttcccgttga gtacaaaaat gaaaataaag acctccaggc ggctattgac
    43561 cttatgtcgg tcagaaacaa tgaggactac cacaacgtca aaataaaaac agatttatca
    43621 atttatggaa gagcctacga gctgttaact gttgaggaaa tagatgacaa aaaaaccgaa
    43681 gtaaaactct accagctccc agccgagcaa acttttgtta tctatgatga tacataccag
    43741 cgaaattcgt tgatggccgt tcatttttac aacatagatt atggctcagg taaacgcaaa
    43801 cagattatca aagtatatac ttccgacaca atttacacct acgaagacta taatctcgaa
    43861 acaaaaggta cgtgcttaaa agagcatgaa gaacattttt ttaaaggtgt accagtcaat
    43921 gaatatgcta ataatgagga gcgtacaggg gcttatgagt ctgtacttga taatattgat
    43981 gcctatgatt tatctcagtc cgagcttgct aattttcaac aagattcagt caatgcgttg
    44041 cttgtaatag ctggtaatgc ttacacaggg gctgatgaga atgactactt agatgacgga
    44101 cgattaaatc ctaatggtcg tttagcgatc tcaattgggt ttaaaaaagc ccaagtgtta
    44161 attttagacg ataatcctaa tccaaatggc gttaaaccac aagcgtactt tctcaaaaaa
    44221 gagtatgata ccgccggtag cgaagcctac aaaaatagac tagttgcaga cattttgagg
    44281 tttactttta cgccagatac acaagatatg aaattttcag gagttcaatc tggtgaatca
    44341 atgaagtata agcttatggc ttctgacaac tatcgcgaga agcaagagag gttgttcaaa
    44401 aaaggcttaa tgcggcgctt acgcttagca gctaatatct gggccatcaa agggaatgaa
    44461 gcaactacat atagccttgt aaatgatacg agtatagttt tcacacctaa tcttcctcaa
    44521 aacgataatg aaattgttac agctgcacaa aatctttatg gcattgttag cgatcagact
    44581 atctttgaaa tcttaaacac tgtcacagga gtagacgctg aagcagagct tgaacgtttg
    44641 aaagaagaag ctgataaaaa gcaatcttta ccggagccta gattggtagg tgatgctagt
    44701 ggtcaagaag aaccaacagc agaaaagcct taattactgg caaaagcgtc aagaagatat
    44761 tctaagttat ttagacagag ctgatttaga tgtcttttca gaactgcaga agttatataa
    44821 tgaacaggct tttgagttac aaaaggaatt gtttgatttt tatactaagt attctgaaga
    44881 gaacaaaatg acttatcagg atgtcgttaa aaagctacgt catgaagatt tatcagatta
    44941 cgtagctaat gctaacaagt atcgcaaaca agctgagaaa gaccctgagc tgctaaaacg
    45001 acttaatgag cagtatgtat cagcaagagc tacaaggatg gatgcattaa atcttgaact
    45061 tgtttatcgc gcagggatac taaaaggtgt acttgattca gcatttgaaa atcatttaaa
    45121 aaaagctgcc tcatatgctt ataaaaaagc aatgggtgga cggtcaggga caatcaatgg
    45181 tccagtttta gaagaactgg ttagaacccc gtttgatggt tataactatt cagaacagct
    45241 gtggggcaat actgacaatc ttgtcaagaa tctccaaaag aaattaaaac aaggttttgt
    45301 tcgtggcgag catcctaggg cgatagccag agatttagct aagcaattta atgttgccaa
    45361 tcacagggct gaaacactaa tcagaactga tggaactatg gtgattaata atgctaccgc
    45421 taggcgctac ttggatgcag gtcttaagta ttatcgtgat ttagttaggc gtgatgacag
    45481 gacaactgag atatgtcgta aaatcgctaa agaaaacaaa agaaagctat tatctgagct
    45541 aaagcctgga attaatgcag caccttatca tttcaactgc agaacaaccc ttattcctga
    45601 tgaagatgag ctaagcattg aagtagaacc aattgatgat aaaagcacta agtattttaa
    45661 ggatgtcacc tcagattgga tagatggaaa tgggcataaa ccacaactgt cactactaaa
    45721 tgagtatgta aaaaatggca ctccttacaa agtagatggc cataatgttg ttcttgatca
    45781 ctcgaattat gaatatagag ttgctaattg gttatctaaa aagacaggat tgcaagttga
    45841 catggtgcca agagttaatt cccctgaaca tatcaatact cctgattatt tagttgatag
    45901 agttcctttt gatctaaaag aaatcacagg ttcgggtaaa aacgtcattg atggcaatct
    45961 acgaaaagca aaaaagcagg caactaatat catttttgat ataacaaaaa cccctttatc
    46021 atttgaagag ataatggggc agttagaaca aatttatatg atagatcgta ggggtcttga
    46081 tatatcaatt atcaaaaata aagatgaggt attagctgta ttagaaaaag aaggatgatg
    46141 ccccaccgcc tccacagtaa actgcttcgt gggcgttaga ccatcatcct tctttatctc
    46201 aattatacat caccctatta aaactatcaa ggaggaaaat atgtttattt ggcaaatggt
    46261 attagtggca ctaggtgtat tggtgctcat attgattgtt ggcattgcag caatagcggt
    46321 taagtcaatt attgcagaat tgaaaaaggt aggataaaca tgaataagcg cattaagaaa
    46381 aaacgtaaat tggaaacagt ggttgtgatg cttgttgcag agaatgccat gcaagcagaa
    46441 gcaattaaaa atcaaaacaa agaaatcatg gagttgaaat caattgttca gcggaacgct
    46501 ctggcaacaa acgaagagtt tgcgtctgtt aaagctgcta ctttagataa tcaagcagct
    46561 attacaaaca ttgcaattga tgttaactac attaagaaaa attataagcg taagtgggga
    46621 aaataaattt taaacgaatt cgaccccttt agaaatcaaa gtcgtagcaa tacggctttt
    46681 attgtgtcca aactttgttg atgacactaa aagctacact gtttcgtcgc aggacgtaaa
    46741 gctagactat cggttggtgg cgtaaccact aggagaaagt tatggcagaa gagaatgtaa
    46801 caacagaaac aactgagcaa gtcgacactc aaaaagaagc tgttgaacat cctaagcagg
    46861 aacatgagcg aacttttact cgcgcagaca tctcaaaaat gatggctgcc gaacgcacta
    46921 aatgggaatc tgaacaatca gaagctattg aaaaagctcg tacagagggt gaacgtttgg
    46981 ccaagttgtc aaaagatgag cgcgctaaag aggaggaaca aaaacgtcta gatgctatcg
    47041 cagagcgtga aaaagcagta gcagagcgcg agatgcgcat tgaaacgcat tccctgcttg
    47101 tggagaaagg attgccattg gattttattg atattgtttt aaccactact gcagaagagg
    47161 ttaaggccaa tattgataat ttacaaacta tttttgataa agctgtcgaa aaacgtgtta
    47221 acgatcgttt aacccagaag ccaccacgaa ctggaaacgg ctcggtcggt atgactaagg
    47281 ctgacatcat ggcaatagag gatgatgacg aacgtatgcg tttaattgct gagaatcgta
    47341 acttatttta agagaggaat attatggctg aaaaaaattt aaacactatg gcggacttag
    47401 gagatattaa atcaattgat tttgttaaca agttttccaa aaatattaat gatttactaa
    47461 aattgctagg ggttactcgt cgtgaaacat taactaacga tctaaaaatt cagacctata
    47521 agtgggaagt gactttagat caaactgatc ctggagaagg ggaaaccatc cctctgtcta
    47581 aggttactcg aactaaagat aaagactata cagtgaagtg gttcaagaaa cgtcgtgcaa
    47641 ctacagctga gtcaattgct cgccatggcg cagctcgtgc tattactgaa gcagataagc
    47701 gcattatgcg tgagcttcag aatggaatta aggatgcatt ctttacattc ctcaaaacaa
    47761 aaccaacaaa agttaaaggc gttggccttc aaaaagcgct gtctgcatca tgggctaagc
    47821 tagctacttt taatgagttt gagggttccc cgcttgtttc ttttgtctct cctttagacg
    47881 tagccaacta tcttggagat actaaagtag gtgcggatgc ttctaatgtt tttggaatga
    47941 cattgcttaa gaactttttg ggtatgcaaa acgtgattgt tatgccatct gtgccagagg
    48001 gtaaaatcta ctcaacggct gtagagaatc tagtttttgc atctttaaat gttaaaggcg
    48061 gagacttggg tggtttgttt gctgatttta ctgatgagac aggtttaatt gctgcagctc
    48121 gtaatcgtca gctttctaat cttacctatg aatctgtttt ctttggggca aatgtgctgt
    48181 ttgctgaaat tcctgaaggg gttgtagagg caacaatcga agctgctgct gtacccggca
    48241 ttggtggtta aaggctattt atatgggcga taaacaactt attgacgata tcaaactctt
    48301 tataggtatt tccaaggatg atggtgcgca agatgagctc atcacccttg ctatatctga
    48361 aagtaaagag cgtgtgctag ctaaacttaa tgaatactca gaaactgaaa tcaccaaaat
    48421 tcctgataga ttgaggttta ttgtccgtga tgttgctatt aaacggttta ataggattaa
    48481 ttcagaagga accgttgaag atagcgaaga aggaaagact tttaagtggg acagttacct
    48541 taaagagtat gaatcaacac tcagaagcgc tgctattggg aaggtatatt caggcaaagg
    48601 ggtagcaaga tttatttagg agatacacaa tgatctataa agatagagta atcttagtgt
    48661 atgtcgatga gcaggacgat tttctagata aaagaactgt tgaaaaacct agcgggaaaa
    48721 tcccctgcat ggaaaatact ttcacaaaat ctgaacagat ggggctattt ggtaaatatg
    48781 atttgaatgc ttttaagttg cacttgcaag gccattacga cggctttagc aagattatct
    48841 acaagggaaa accgaggttg ataaagggac tagcacacca taaaaatagc acggttattt
    48901 atgtatgagt cttatttatc ggatgagagg cctagatagg tttttacgca gcgttgagcg
    48961 caagcagaag tcagtacgaa tcgctgtaga taaagagctt agtaaatcag ctgctaggat
    49021 tgagagacag gctaaaatac tagccccggt tgatactgga tggctgagag ctcaaatcta
    49081 caatgagcaa caacgactct tacactatag agtggtttct cctgctttat attctattta
    49141 tcttgaatta ggtactcgta aaatggaagc tcaaccgttt ttagaccctg ccctgagaaa
    49201 agaatggcct gtgctaatgg ctaatctcaa aaaaatgttt aagaggtgat gcatggatta
    49261 ctcaccagaa acactatatt taaaaaaggt aaaaaataga ttgggagctt taggcatacc
    49321 aatctatttt aaattgccta aatcagacgt tttagagcct tttattgttg taggtacaaa
    49381 tatatcagac ttgtcaaaaa cagctcaaac tggagcagtt attgacgatt ttagcctgaa
    49441 tatcgatgct ttcttacctg gcgatagtcg tttggatgca gaagagatta aagctcgcct
    49501 gcttagacta cttgggcgga ataaccaaat aaaggctcag attttggtag ataattcaat
    49561 aggacgagaa gtctatagag ttgctatcaa cattacagaa acactatttt aaaggagacc
    49621 taaatggcaa acactaaaga aggaacaact attgaaatta caacaggtaa accgattgta
    49681 gggaaaaaga ttttttattt catccaatca gttgatgcta aaaaaggtag caaggctttg
    49741 ctgccagcct atcgtaccga tggtactaca accatgggcg gtgaatacat tgatgagcaa
    49801 acccaacaag gccgcgttat tgagaaggct acagatgaac actctattga tttgacaacg
    49861 tactttgtac cgacagatcc atctgttgct gtcatcgagg aagctaagaa aacaggtaaa
    49921 tctatcaaaa tttgggaagt cattgccgac gaaagtgtca aagaacagat ccaaatttct
    49981 gaatcggctg gacccaagaa agatgtttac cctgctaagt ttgggtacgc caagattgac
    50041 gaaatcgaac gcggtactgg tatttcagac ttagtagaaa tgtcctacac cgctaacatt
    50101 gtaggggcct tacaagatgg taaattccct ctcacgaaag aagaaattga aatgctagaa
    50161 aacgtatacg gctatcaaaa tccgggtgat actactggcg actacgataa tatcactaag
    50221 tagtacacag gggcggcaac tgctgcccca ttttgattta aaaaggagat aaaataatgg
    50281 aatttacagc agcgaaacga aatatcgata ttaaatttga ttttaaaact atgtttaaga
    50341 tcaataataa actaggaaca attaacccag aaacaggaga gcgcaatgca gatggtgttg
    50401 gtgctttgtt tttcaatatc ttagagcgta atgaaagcgc cattgttgac cttgtgcgtt
    50461 tatctgcggg aagcggaaaa aaagcgctaa ctgaagatga aattctagat gcaattgcag
    50521 aagctgttga tgaagaagga acaacagaag ggttgtttgc tgaaatagag aaagaaatgg
    50581 ttgattctgg ttttttcaga gcgaagattt tgaaatatat cgaaaacatg gagaaatcag
    50641 ctcgttatct caaagcgaag gacgacacgg acgcaactca aatccaaatt atcgaagaca
    50701 tgattggaag aatgagcaac gcagtatctt agtaaattgc gcacggttag ggctcacaga
    50761 cattgatact atctataaat gtactaagtg ggagctagag gctattatgg aggggcttga
    50821 gtataagcag gttgctgagc gcgagaacct ctctgaattg tctctaaaac ttagatatac
    50881 attaaatgct aaaaaggttg atgtaggtaa gctcaaatac gataagcata ggctaactat
    50941 caaaagatcg tatcaaaaag ctagccgtag ccaagccgat agtgatagca gtattgttga
    51001 gagaatacaa atgcttaata atcattttca aaatagatag ataaggagga gtagatgcca
    51061 ggaacgtttg atggttctat tttcgctgat attggtgcca acacaaagga ctatgagcag
    51121 gccatggctc gtattgttag tacgactcag aatgctttca gaaaagccca agatacagcg
    51181 gttaacagta gtaataaaat ggttcaaatc attggacaaa tcatggccca attagcgaac
    51241 aacggcgaat cgctcggaaa gagacttggt tccgcctacg ctactggttt aaaactgagt
    51301 attggtgaaa ttcaacgcat agcagcttcc attggcgaaa aaattcccga acccataaag
    51361 aatggttttc aaaaaatcat tattccagtt gcaagtgtta ttgacaaaat acggggtaac
    51421 ttaaccggtt tcattaatca aacaaaaatg caacttcaga atcttgtgga tattggtaag
    51481 ataagatctg catttgccaa ggctgcgact ggagttgata tcttaactca gagggcaagt
    51541 agtaagctta ataacttatc tgcaatattc gaaacggtcg caggcaagct tccgcgacca
    51601 ttcggaacag cgtttagaaa aattgcctca tccgtttcta ccctgaatag ctctatacag
    51661 tctgttggag ggaagatttc taattcactt ggtcggcaag tcttgaaccc agccttacag
    51721 tcatggaata atttttttag ttctgtatca gctaaggcgt ctgctttcgc aaacaaagtg
    51781 agtactagtt tgtttggtcg gctgacctca agtttagcta atttatcaag taaaataggg
    51841 agtagtctta gcaatggttt tagcagaatg tctagttctg cggctacttc cttgaatgga
    51901 atcagccaga aattcgccaa cacctcttct gctggagaga gactcaaaag tacagtgatg
    51961 agcatcgtgc aggcctttag tttaatggct gttgttcaaa aagctatgca cgctattact
    52021 ggggcaatag acggggcggt tagtcgcgtt gataccatga accgctttcc gaaaacgatg
    52081 gcgctatttg ggtattctgc tgaacaatct aaggcatcaa ttgataagct atcaaaagga
    52141 attgaaggtc tgccaactcc tctagacagt gctgtaaaaa gcgctcaaca gctcgccata
    52201 accacaggaa gtttagataa agggactagt ttagccctcg ccttcaataa cgcaatgatc
    52261 ggttacgggg caacaactga aggggctgag caagcactca ggcagtttaa ccagtcgttg
    52321 gggtctggaa aaattcaagc tgaagagttt aactctgtat cagaagctgc gccaggttta
    52381 atgtctaaaa tggcggaagc cttcggtttt ggtaaaaatg gcgtacaaga tttaaaatca
    52441 gccttatctg atggcaaaat cactgcacaa gagttcgcag ataaaatgat tgagcttaac
    52501 gatgcccaag gcggatttgc agagatggcc caatcatcgg ctggtggaat ccgaactgcg
    52561 tggaagaacg tacataccgc cgttgtaaaa ggcgtagcag gtatgatttc agcctttgat
    52621 gaagcggcta aagctaatgg tatgaagacc attgctgaaa cacttcttag cctgaagccc
    52681 gcaataacca gcgtttttga tacgattaat tctcttattc caaatgcagt tgcagcattc
    52741 gctaggctaa aacagtctat caatattgac tttagcccat taggcgccag cgttaaagag
    52801 gtgtttgctc ttattaatac tgttcttgga gattttgcac atactggtga attatcaggg
    52861 caggcttttg ataatctaaa agcaaaaatc acttctttag cccctaaggt cattgccctt
    52921 tgggcagtga tgaaccccgc tagcgctata gcaacgataa tgcctttgct ttctctattt
    52981 ggaaaagttg gtctagcttt aggaagctta ggggcttctg tcggggtgtt tggcggcata
    53041 atttctagtg ggatagctag tgccagcggc gttgttggtg cctttgcggc aactctaagc
    53101 ggattacctg gcgtttttgc taccgcagca gggcgtgggc tatctgtgct tggaactatg
    53161 acaagcgcaa tgtccagtct tgtgagttta gcactggcgg ccataggacc ggccgctatc
    53221 ctaggacttg tggtggcagg acttggtttg attaatagcc agtttggcgc acaaatcgac
    53281 cagctactta acactgctgt aacaaaaggc cctagcatta ttcaaggcct tgtaaaaggg
    53341 attacttcta aaataccagc tttgatagct agtggtactc aattgattgc taaatttgca
    53401 aatgctatca cagtgttatt accggttata attcaagctg gcgttcagct aattaccagt
    53461 ctcgtccaag gtattggaca aaatgcaacg agtttaatta gttcagcaat aaaaatcatt
    53521 ggtagttttg ttagctcaat tgcgagtgcc ttgccgcagc ttatttctgt tggaatggag
    53581 ctattattaa atgttgtcaa cggcatagtt caaaatatac ctcttattat ccagcaagca
    53641 cagcaaatca ttggtagttt tgggaacagt ttacaggcta accttcctag tattatcagc
    53701 cacggtatag ctattttggt gaatcttgta cagggaatta ctcaaatgtt gccaactgtt
    53761 ctgcagatag ctactcaagt tattacgagt tttgtatctg gaattgtaca gtttttaccg
    53821 cagttacttc aaggtggcat tcaaatcatt attagccttg tgcaggggat tattcaaaat
    53881 ctaccccaga ttgtacaatc tgctgtacag attatacagt ctttagtttc tggactgacg
    53941 caagctctgc ctcagattat cgcagcgggc attcagcttg ttgtacaact agccgtagcc
    54001 ttgataaagg gacttccaca gattatttct gcgggtattc aattaattac gggactcggt
    54061 aaagccatgt tagaggctat cccaaatgct ctttcaggag tttgggaagg tattaagagc
    54121 ggattcagtt cgatgtggga tcaaatcaca ggtaagagtt ccacaagtac agctaaagtc
    54181 tctgcggacg ccacggccat ggccttgaat gttggtactc aaacaacagc tatggctaat
    54241 caagccaata cagatacgac atctatgctt aatagcatta gtcaaaatac agagcttgct
    54301 aatataaacg ctacatcaaa tgctacacag atggctaata atgttaatgc tcagacagga
    54361 acgatgagtc ttcaggggtt gaatgattca atgtctcttg cgagtggcat tagcgctaat
    54421 atgtctgtgg ctaatattaa cgctactact caggctcaag aaatggcttc aggggttaat
    54481 ggtgcgactt cttcaatgaa tctagacgcc attaatcata cgctcagctt agctagtggt
    54541 gttggtgcaa acatgggaac cgctagcaca agtgcaactt ctcaggcgca agctatgagc
    54601 tcaggagtga gtaacagtct ggcatcaatg cagtcaaact caactaaagc agcctctggt
    54661 ctgtctaata gcgtgacaag tgaaatgtct tctgcagcaa cctctgcaac ctcaagcgcc
    54721 aataggttgt cctcggcggt tgagtctggc ttcaataagg cgaagacttc agcaacaacc
    54781 tcaatgaatg ggatagccaa tgcggttaaa acaggattta gtagcattaa cagtactgca
    54841 aagcaatcta tggcaaacct tgtaagttca gtgacttctg ggatgtctcg tgcaaccgcg
    54901 gtgtctaata atgcttgtca gaagatcctc tcaatattca gggctttggc agggcagatg
    54961 tcttctgcag gagcttatgc aggtcaaggt tttgctaatg gtctagctag tagcgcaggc
    55021 acaatctacg caatagctaa cagcattgcc gctaacgtag cagcaacaat tagacgcgct
    55081 ctggatattc actcgccatc tcgtgtcact aaaacactag gagcatttac tggagagggg
    55141 tttgccctcg gtatggcaga gtggataggt gagatcaata gtctaggtaa agcatacgca
    55201 acagcagtta ctgatcaaaa ctggggggta aatagtactg tttcaacatc tgctaaagtt
    55261 aataatagtg gaatcaatac ctctcttgat aatcttagcg aagaggttag gcagtctcaa
    55321 ttgtcagagc ctatctttga agtgcataat gagattgttg gagataaaat ttacacggct
    55381 gttaaagaaa aagaatcaag agagcaatct aaagattctt attttgtttt tgcctagaag
    55441 ggagtatttt ggatttatta atagaaaaag agagtcaggc aactagattg tctgactttg
    55501 gtatttataa tattgctatt gaagatagtg ctcctctact atctgtatca caccgagcag
    55561 tcaaaggaag gagtggttac atctacgatg gggctacttt tacgactaaa acgcttaagg
    55621 tcaaaggaag agtgacggta agcgatgtag aaggagtttt ggacaaacag gacgaactaa
    55681 acgctctgct tgttgttgac gaacctttct acgtgactaa gatgtaccct gaaaactcag
    55741 atctatttaa ctttgagctt ccaggggaaa gcacaggaga cctgcaactt attggctctc
    55801 ctcataaacc ttggaaatat agatttaaag tcatcctaga cgacactatc gcttatgaat
    55861 ttataggaaa aaccagtcaa ggactaaaat ataatctttc cttcacacta agaactgctg
    55921 aattaccttt cggtgagaca aaaccaaagg acataactct atcagggggc agttttgcct
    55981 atggaggaac cgctaaagca agtcagttag agtggccttt tattatagag cttactccct
    56041 ctggaggtca gactaatttt tatattgaaa ttgatggcag gcgctttgag tttaagcaga
    56101 atagccagct acaaaatagt gataaattac ttttgacagg catagctacc acactaaacg
    56161 gaaattatgt caatgctaaa acgaactatg agtattttat cttcaatcct aatcctaaca
    56221 agagaattac ttacaaaacg gattttcttg gcacgattag gattttaaat tttgtagagt
    56281 tgtataaata ggagggtcta atgattactt ttttggatca tcgtgacatt gagtatgggg
    56341 ccattagtgt cattaggcat actaatgctg ttaatggtga gcgctcagtg agcggggaaa
    56401 tttatacaaa cagtgatgtc ctcaataata tcgatagagg gtggcgactt agatttgaag
    56461 atgagtatta cgttgttatt tacgcaaagc ctgttgatgt tggtcaaaaa acgcaagttt
    56521 cgtttgatgc catacaccaa tttttttggg acttcagcaa gagtagtatc tatgagggtc
    56581 tcggagatgg gtctcatacc attgatacct atctagagac agtatttaaa ggtagtgggt
    56641 accgctataa gctagaagta ggtgtgaacg catttagaaa acaatctttt aattataagt
    56701 ctaggttaga tttattcaat gagattatta aagcgaccgg acttgagttt tcagtcagcg
    56761 ggaaagttgt tcggctatta aaaaacatag gtactgatct gtcaacagtg gtacgaaaaa
    56821 atttcaacat gaatgatctt accattgaaa aaaatattga tagctttatt acctatcaga
    56881 aaggctttgg tgcatggacc gacccagagg accactctaa ggggagactt gaggcggaat
    56941 acgaaagccc tcttgccaag gagtacggtc gacttgaagg ggcgcctctg acggatgagc
    57001 gcttcacggt ggcagataat ctaaaagaag ctttaaaaag taatgttgaa aattcttata
    57061 agatatcagt aaaaattgat atggaagatt taatgagagc tggttataga tgtgagaggc
    57121 cggttgctgg tgactacatt atggcgataa atgagacttt aggttttcaa gagcgtatac
    57181 ggatagtttc ttttacaagc tactacgacg caactggagc tttagtaaaa catgaagtca
    57241 cttgtaatga tatcggatct gtgaaaaaac aaagcgtagg aagtctgtct ataaacagta
    57301 gaatcaatca gatagatgca gatattgctt ctgcaattga agtagcaaca caagcactag
    57361 tatctgctga tgggaaaaat actgtttacg gtggcacaga aatgcccaaa gatgaaccaa
    57421 aaggaacctt aaaaaaagga gacatattat ttctaaaagt tggtgatact actagaatgt
    57481 acttttggaa tggtgctgag tgggaggaac ccgaggttgt gaacgaccca gaacgatggc
    57541 gtgaagacct cgaaaaacaa atctctgagg caatcgaaaa agccaaaaaa gcgcaagaag
    57601 aaatcaaccg gcgcactgac aaagagcttg aagagtttag agaaacactc aaaaacctag
    57661 cgttaccaga agaagccatt aaaaaaatca cagatgctat caaggttgat gacatcccat
    57721 cgattaaaca aagctttgac gacctcaaaa acaaagtcag tgaaacgagc gagacatccc
    57781 gtctaaacgc cgaaatcata ggcacagacg gtaagacccg ctacaacaaa aatttgctgg
    57841 tcggcaaccc taatcgtgtt aaaaaaattg atgaggattt tattgaggta gaagccaatg
    57901 atggtggttt caagcgtggc gagacctata cgattagctt tagccagaca tgtgagccac
    57961 taaataaagt ggcaattacg ctgatacagg ctaataataa aggtctcaag ctggtgctga
    58021 caccgacaaa ggctaagatg gagccacaga cgtttgaggt cactaaggat aaacagtcta
    58081 tagaggtcta tcctttgagc tataaaggtg ttttaacagg tgattggtat aaatctaagc
    58141 aaatagattt aaccgcgtca gaggggcaaa ttttagctct ggatatggct tataaagctg
    58201 tggttgatgc taaaggtgca acaatcacag ctaaacaatc aagcaatcca aaaattattt
    58261 tagacggaag gagggacaga tgacgctaga agaaagaata ccaattaaag tcttatttga
    58321 ccgcaaggat gccgccgaat ggcaaaaatt aaatcctgtt gttgatgatg gtgaactagt
    58381 tgttgagcta gacactcaca ggctaaaggt cggagatggc aaattgaatt acaacgactt
    58441 gccttactac gaaggtcctc aaggagaatc tatcactaaa gtacagctat ccgaaaatgg
    58501 tgacttgtct gtgtggattg gcgacaaaga gactaagctt ggcaacatca aaggtcaaaa
    58561 aggtgataaa gggacaagta ttgccgacat cactaaagtg ggtgagacgc ttactatcac
    58621 actatcagat gacactcaaa aaacctttag tatccctaat ggccaaaaag gggatagagg
    58681 aaaaggagta gagagtgctc gagttgatga agaggggaac ctttttgtca agtttgaaga
    58741 agaatctgaa aagttagtag gcaacatcaa aggtcaaaaa ggtgataagg gtgattcctt
    58801 aaagtttgaa gaccttactt ctgaccaaat cgcccaaatt aaagctaagg acgtagacct
    58861 atctgcctac gctacaaagg cagaacttaa agagattgat gtgagtaagc agcttgcaga
    58921 ctatctgtct aaagcagagg caaacagcac ttatgctaaa gcaagtcaca agcactcgct
    58981 aagtgatatt actgatctaa acctagaccg gtatgcgact aaaatagagc tacaaaacaa
    59041 agcaagtaca tatcaccggc acagaacatc tgatattgat ggtatagatg aataccttaa
    59101 acaaactgat ctgccacaag acattgttaa gcaatcagat attagggacg ttgtccgaaa
    59161 tgcgcagcta agcggctatg ttagattagc tgatatccag tatcagttaa acaacatcgg
    59221 aaagctaaaa gatgtggcaa caggtcagta cctatctgtc cgtgtggttg ataaaggtaa
    59281 agtgccatat aacacaagcg gattaattgt ttttgaacgc tctggaggta agtaatggca
    59341 gtgcaagaga taccagacac gtatttttac cgcttagaca gtcaaagcat tgatggtact
    59401 ttattacaaa aagccagttt ggttgtcgac caattttata ttaagataga tgattactat
    59461 agcttttaca cagatcaaga cgttgatgta tataaaatta tatctagcac acaggctgat
    59521 gtgtataaag ctaataagta taactatcag tcattatcaa aaccagatac tagcgtccaa
    59581 aagccttctg ccgcgatggg tttgtaccaa tcgattacta ttgatggact gccaatagct
    59641 cgcgttattt gtaataatca ggtcatttgg tctcaatcag cagagcctca ggaagtttac
    59701 aacattacag cgtatgtaga cgctaacgta tctaaattaa atgattatgc tgtcattgat
    59761 tacaatcagg attttgcgac ttatggggtt gtttggcatc gagttgataa agtcgctgtt
    59821 gaggttgacg gcaaaatcgt tgctaagcta caaaaaccag ttcggacagt tgccggcgga
    59881 ggcgcaatgc tgtctatcga gacgtggact tatccgtttg atgggataga caagtataaa
    59941 aacccaacat ttaaaatttc gcaacattag gaggtaacaa ttgagtagag atccaacact
    60001 tttaatagac gagtcaaatt taacgattgg ctctgacgga cgtgcttatt atacatttaa
    60061 agctgatggt gacacaaaaa gcgttaaatt agctaataac aaatgtatcg gtacgactcg
    60121 ctttaaccag ctcatgattg agcgaggggg taagccaact aactacgtgg cgccagtggt
    60181 tgttgagggc agcggtgagt caaccggact ttttaaaaac ttggaaggag cgctcagtca
    60241 gttaaaagag cttaatttag agctgacaga taccgttaat tctcagcttt gggcaaaaat
    60301 caaactcaca acaaacggca tgttgcgtga gtatcatcgt gataacatca caacagagat
    60361 tgtcgaaagt gcaaatggta tagcaacacg gattagtgag gatactgata aaaaacttgc
    60421 gcttattaat gagacagtct caggtattag acgtgactat caagacgctg atagacagct
    60481 atcatcaact tatcaagctg gcattaacgg cctaaaggcc acaatggcca atgataaaaa
    60541 cgacctaaaa gctgtgatac aagcaaccgc tcaaggtttg tcgcaaaagt atgatgatga
    60601 gatacagcag ttatctgcta agataaccac gacatctagc ggcaccacag aggcctacga
    60661 gagtaagctt gcggacttac gagctgagtt tactcgtagc catcaaggca tgcggacagt
    60721 gctagagtca agaatcagtg ggttgcaatc aacgcaacaa tcaactgcct accaaatctc
    60781 acaagagatt aaaaatcgtg agggtgctgt tagtcgtgtg caacaggacc tagctagcta
    60841 tcagcggcga ttgcaggata ctgagaagaa ttacagtagc ttaacccaga cagttagagg
    60901 gttacaatca actgtcagcg cccctaaccg agggttagaa tcccgtgtca cacaactagc
    60961 tggcttgatt gaacaaaaag tcactcgtgg tgaaatggag agttatattc gtggggcagg
    61021 cgacagtatt atgcttgcga ttaagggcaa actcccgcaa agcaaaatgt ccgcaagtga
    61081 gattgtctca gccattaatt taaacggcta cggtgtccgc atctctggcg agcgtatcgc
    61141 cttagacggc aataccacgg ttaatggagc gtttggcgca aaacttggtg agtttatcaa
    61201 gctaaaagcc gaccagatta tcggtgggac aatcgatgca aacaaaatca atgtgattaa
    61261 tctcaacgct agcagcattg ttggtttaga tgctaacttt attaaagcaa ggattgagca
    61321 tacgattaca agtttgcttg agggtaaagt catcagagca agaaatggtg ctatgatgat
    61381 tgatttaaac aactctacca tcgattttaa tagcgatgca tccatcaatt tcaacagcaa
    61441 caataatgcg cttgttcgaa aatcaggtac ccacactgct tttgtgcatt ttggtaatgc
    61501 gacaccaaaa aactttacaa ggtcggctct ttatgcgtca atcggaatca cttcatctgg
    61561 tgatggtatc aatagcgcgt catctggtcg tttttgtgga gcaaggtttt ttcggacggc
    61621 tagcggatat gaacatacag catcagttga tcaggcagaa atttatggag ataccatcat
    61681 ttttgcggat gactttggta taaaccgtgg atttaaaatg acaccaacgg gtgtaaacac
    61741 acttgtggat atcaataaaa tgtattatgc gattgtagct ttggcaaggt gctggaaaca
    61801 tctacaaaat gttggctggg atactacaca cccgaatttt actagcgcta tcatgaacga
    61861 gcatagcaat tatatgactg gaatttagga gaaacaatgc aagaaaaatt attaggaaaa
    61921 attattaacg atttaacact taaagtcgct aatttaacgc tggaaaatgc tcaattaaaa
    61981 gcacagcatg aaatcgaatt ggaagaatta aacgcacaat tggatgaagc aacagcaccg
    62041 aaggaagaag gtaaataa
//
LOCUS       DAUDYX010000005        55147 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.005, whole genome shotgun sequence.
ACCESSION   DAUDYX010000005 DAUDYX010000000
VERSION     DAUDYX010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 55147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55147
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.005"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            1..1125
                     /locus_tag="VHD02_000304"
     CDS             1..1125
                     /locus_tag="VHD02_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEQ5496942.1"
                     /translation="MTAGVQLLDEKDFSNIKEKNQLVQLVTKSNRPIGTAYISKQNKG
                     IGWYLGPEKIDLSISYFVSLFSVAKAKRQDFAQSDETNAYRLFNQEGDGFGGVTIDLY
                     KDFAVFSWYNAFVYDKKEMIMEAFQQVFPEVKGAYEKCRFKGPDTETAHLYGELAQET
                     FSILENGIAYQVFLNEGLMTGIFLDQHDVRRALVDGLAMGKSLLNLFSYTAAFSVAAA
                     MGGAIETTSVDLAKRSRELSLAHFEHNQLNLASHHFVVMDVFEYFKYAKRKKLIFDVI
                     VIDPPSFARNKKQTFSVSRDYHKLITEALDILSPKGTIIASTNAANMTVSQFKKQIIK
                     GFGSRRPESMTLQQLPSDFTINKADERSNYLKVFTIKVRE"
     gene            1122..1808
                     /locus_tag="VHD02_000305"
     CDS             1122..1808
                     /locus_tag="VHD02_000305"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEQ5496943.1"
                     /translation="MRIVAPVMPRHFDEAQAIDISKYEDVNLIEWRADFLPKDEIVAV
                     APAIFEKFAGKEIIFTLRTVQEGGNITLSSQEYVDIIKEINAIYNPDYIDFEYFTHKS
                     VFQEMLDFPNLILSYHNFEETPENLMEAFSEMTKLAPRVVKIAVMPQSEQDVLDLMNY
                     TRGFKTLNPEQEFATISMGKLGRLSRFAGDVIGSSWTYVSLDHVSGPGQVTLNDMKRI
                     IEVLEMDISN"
     gene            1902..3068
                     /gene="aroC"
                     /locus_tag="VHD02_000306"
     CDS             1902..3068
                     /gene="aroC"
                     /locus_tag="VHD02_000306"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEQ5496944.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLTLHPADIDHELQRRQGGYG
                     RGARMSIETDRVQISSGVRHGKTTGAPITLTVINKDHQKWLDVMAVGDIEETLKLKRR
                     VKHPRPGHADLVGGIKYHFNDLRDALERSSARETTMRVAVGAVAKRILAELGIDMLHH
                     ILIFGGITITIPSKLSFRELQERALHSELSIVNPKQEEEIKTYIDKIKKEGDTIGGII
                     ETIVQGVPAGLGSYVQWDKKLDAKLAQAVLSINAFKGVEFGVGFDMGFQKGSQVMDEI
                     TWTPTQGYGRQTNHLGGFEGGMTTGQPLVVKGVMKPIPTLYKPLMSVDIDSHEPYKAT
                     VERSDPTALPAAGVIMENVVATVLAKEILETFSSTTMSELQKAFSDYRAYVKQF"
     gene            3129..3470
                     /locus_tag="VHD02_000307"
     CDS             3129..3470
                     /locus_tag="VHD02_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEQ5496945.1"
                     /translation="MSQEIYDYANQLERAVRALPEYQKVLEVKEAIQADASASELFDE
                     FVAMQEKIQGMMQSGQMPTAEEQTSIQELSQKIEANDQLKAYFEAQQALSVYMSDIER
                     IVFAPLKDLVK"
     gene            3691..5043
                     /gene="gorA"
                     /locus_tag="VHD02_000308"
     CDS             3691..5043
                     /gene="gorA"
                     /locus_tag="VHD02_000308"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEQ5496946.1"
                     /translation="MVIPYDYIVIGGGSAGIASANRAAMHGAKVLLAEGKKIGGTCVN
                     LGCVPKKVMWYGAQVADILGTYAKDYGFDFKEKAFDFKQLKANRQAYIDRIHASYERG
                     FEQNGVDRIYDYAVFKDAHTVEIAGQLYTAPHILIATGGHPVFPDIEGAQYGISSDGF
                     FALDEVPKRTAVVGAGYIAVELAGVLHALGSKTDLFIRHDRPLRSFDKAIVDVLVEEM
                     AVGGPCLHPHSEVAKVVKNTDESLTLYLKDGQEVEVDQLIWAIGRKPNLEGFGLDKTG
                     VTLNDKGYIETDAYENTSVKGIYAVGDVNGKLALTPVAVAAGRRLSERLFNGKIDEKL
                     DYQNVATVIFSHPVIGSVGLSEEAAVKQYGQEAVKTYQSRFTSMFTAITNHRQPCLMK
                     LVTVGDTEKIVGLHGIGYGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSEEFVTMR
                     "
     gene            complement(5131..6399)
                     /locus_tag="VHD02_000309"
     CDS             complement(5131..6399)
                     /locus_tag="VHD02_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEQ5496947.1"
                     /translation="MTTYDSISWIHTFKANGRKTDLARMAWLLEALGRPQDKFPAIHV
                     VGTNGKGSVTAYLQHILSTSGYQVGTFTSPFIISFHDRICLNGQPISDLELAQCVSVI
                     RPILTKMSLETNWDRPTEFELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHAL
                     AVVCPSISFDHQERLGYSLAEIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGS
                     RLYQLGKDFLLNPSEKTFSFQHDNLNLTKLRLKLLGQHQKANACLAIMTAQLLRKSFP
                     KISPKTIQKGIEATTWPGRSEFIQPNLLLDGAHNPDSIAKLKSLLQEEFPKRPIHILF
                     AGLKRKPLADLLAQLDTFEVSVTTFDFPEANLLEDYPDKYPQVKDYRDWLQGSVTSDE
                     LFVVTGSLYFISEVRQYCLSNANIFKNIFP"
     gene            complement(6429..6869)
                     /locus_tag="VHD02_000310"
     CDS             complement(6429..6869)
                     /locus_tag="VHD02_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496948.1"
                     /translation="MSSRYSRQQKPRKSGGVPTEHIKTGFTAFQKSIALIGSILSIIV
                     ATITISRALQPVPDKTSNDTPKESSNTIVKIIEKESSQSNQNHTGTSETTDDNKTTLP
                     SSSTAPSHNETTPTAPETPSTTEGTQLPNATGAEDANTGLPTKP"
     gene            7104..8246
                     /locus_tag="VHD02_000311"
     CDS             7104..8246
                     /locus_tag="VHD02_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ5496949.1"
                     /translation="MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRI
                     LEASRKQIAGLLGVKSEEIFFTSGGTESANWAIKGIAFEKTAFGKHIIISAIEHPAVS
                     ESVKWLLTQGFEVSYAPVTTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPIRAI
                     SNLLANQPTITFHVDAVQAIGKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGK
                     RLNSLLSGGGQEQELRSTTENVAGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKALS
                     AYPDVTVFSAQEGFAPNILTFGIRGVRGEVIVHAFEKYEIYISTTSACSSKAGKPAGS
                     LVAMGIPVKTAQTAVRISLDDDNDMGQVEQFLTIFKQIYDKTQKVR"
     gene            8258..9472
                     /gene="thiI"
                     /locus_tag="VHD02_000312"
     CDS             8258..9472
                     /gene="thiI"
                     /locus_tag="VHD02_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEQ5496950.1"
                     /translation="MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAIAVR
                     SDRDRTHVSLNGTDYQPIVEALKLVFGVQALSPVYKLEKSVPLLVTAVQDIMTSLYRD
                     GLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAY
                     ISYEEIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPG
                     ALAKAQDLTRRLTRFGGNIQFIEVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIR
                     EQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMDKLEIIEMAQAIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKLGNAEKYEERFDIDGLVQRAVSGIVVTEITPEIV
                     NDEVENLIDALL"
     gene            9510..10802
                     /locus_tag="VHD02_000313"
     CDS             9510..10802
                     /locus_tag="VHD02_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEQ5496951.1"
                     /translation="MILLKSLYKGFFAAMKEENFEARCYNREKGGCVLIKTMQYKKTM
                     ATVVALIVALLLFGLIYDLLGVQKNKLAAQKSAQPKVKTARVVANGDILIHDILYMSA
                     RKADDTYDFTPYFEYVKDWISGADLAIGDYEGTISPDYPLAGYPLFNAPEEIAGALKN
                     TGYDVVDLAHNHILDSQLDGALNTKKVFHQLGIDSIGIYDKDRSKESFLIKNVNGIKI
                     AILGYSYGYNGMEATLSQEDYEKHMSDLDEAKIKKELQLAEKKADVTIVMPQMGTEYA
                     LEPTAEQKELYHKMIDWGADVVLGGHPHVIEPSETVIKGRQKKFIIYSMGNFISNQRL
                     ETVDDIWTERGLLMDLTFEKKDNKTKIKTVEAHPTMVLAKGKGIVGKEGFELYNYRTM
                     VLEDFIKGGKYHDKIDEETKAKVALAYQEINDLVNLKW"
     misc_feature    10907..10981
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            11016..11330
                     /gene="rplU"
                     /locus_tag="VHD02_000314"
     CDS             11016..11330
                     /gene="rplU"
                     /locus_tag="VHD02_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEQ5496952.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKIDAEAGAEVTFNEVVLVG
                     GDKTVVGTPVVEGATVVGTVEKQGKQKKVVTFKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            11342..11668
                     /locus_tag="VHD02_000315"
     CDS             11342..11668
                     /locus_tag="VHD02_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEQ5496953.1"
                     /translation="MIKAIFTRQKNGQLSSVTLTGHAGSGKHGFDIVCASVSTLAINF
                     VNSLEVLADCQALVDLNDVEGGYMAITIPPHDNKEEVQLLFESFLLGMTSLAKDSSKF
                     VNTQVI"
     gene            11696..11989
                     /gene="rpmA"
                     /locus_tag="VHD02_000316"
     CDS             11696..11989
                     /gene="rpmA"
                     /locus_tag="VHD02_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEQ5496954.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPVAK"
     gene            12337..13251
                     /locus_tag="VHD02_000317"
     CDS             12337..13251
                     /locus_tag="VHD02_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ5496955.1"
                     /translation="MNIQQLRYVVAIANNGTFREAASKLFVSQPSLSVSIKDLEAELG
                     FQIFNRTTSGTVLTSQGLVFYEKALEVVKSFDSFEKTFSQADLDQNEFSIASQHYDFL
                     PPLITAFSQQYDGHRVFRIFESTTIQILDEVAQGNSEIGIIYLNVDNQKGLFQRMDKL
                     GLEYVSLIPFTTHIYLSKTHPLANREALYLNDIQGLPAVRFTQERDEYLYYSENFVDT
                     SECPRIYNVSDRATLNGILERTNAFATGSGFLDHRSVNGIKVIPLADHIDNQMIYVKR
                     KDKNLSVAGATFVTILKDYFDKRRKALT"
     gene            13248..13706
                     /locus_tag="VHD02_000318"
     CDS             13248..13706
                     /locus_tag="VHD02_000318"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEQ5496956.1"
                     /translation="MKKRLFVLSLILLVALDQLSKFWIVSHIALGEVKPFIPGIVSLT
                     YLQNNGAAFSILQDQQWFFVVITVLVIGYAIYYLATHPHLNIWKQLALLLIISGGIGN
                     FIDRLRLAYVIDMVHLDFVDFAIFNVADSYLTVGVILLVICLWKEEDYGN"
     gene            13696..14586
                     /locus_tag="VHD02_000319"
     CDS             13696..14586
                     /locus_tag="VHD02_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEQ5496957.1"
                     /translation="MEINVITSGQRLDKALADLSPLSRGQANDQIKQGLVLVNGQQKK
                     AKYTVQAGDVICFELPKEEVLEYQAQNIPLDIIYEDDALAIINKPQGMVVHPSAGHPS
                     GTMVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKTDAAHQALAEELKAKKS
                     LRKYLAIVHGNLPNDRGMIEAPIGRSEKDRKKQAVTAKGKEAVTRFTVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDPLYGPRKTLSGHGQFLHAKTLGLTHPMTGKEM
                     IFTVEAPEIFQKVLKLLRKK"
     gene            14982..15503
                     /gene="pyrR"
                     /locus_tag="VHD02_000320"
     CDS             14982..15503
                     /gene="pyrR"
                     /locus_tag="VHD02_000320"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEQ5496958.1"
                     /translation="MKTKEIVDDVTMKRAITRITYEIIERNKQLDNVVLAGIKTRGVF
                     LARRIQERLHQLEGLDLPIGELDIKPFRDDMRVEEDTTLMSVDITGKDVILIDDVLYT
                     GRTIRAAIDNLVSLGRPARVSLAVLVDRGHRELPIRADYVGKNIPTSSVEEIVVEVVE
                     VDGRDRVSIIDPT"
     gene            15519..16778
                     /locus_tag="VHD02_000321"
     CDS             15519..16778
                     /locus_tag="VHD02_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEQ5496959.1"
                     /translation="MKDVIYDVEEVPKAGMLVGLSFQHLFAMFGATVLVPILVGIDPS
                     VALLSSGLGTLAHLSVTKFKIPAYMGSSFAYIAAMQLLMKTNGIGAVAQGAMTGGLVY
                     LIVALIVKAIGNDWIDNILPPIVVGPIVMVIGLSLASTAVNDVMLKNGNYNLTYLVIG
                     LVTLLSVIFFNIYGKGIVAIVPLLLGLLVGYVVALLVGVLTGQEIVDFTNVAQAKWFS
                     IPSVEIPFLTYGVKFYPSAILTMAPIAFVTMTEHFGHIMVLNSLTKRDYFKDPGLEKT
                     LTGDGFAQIIAGFLGAPPVTSYGENIGVMALNKIFSVYVIAGAAVIAALLSFIGKVSA
                     LIQSIPTPVIGGISVALFGVIASSGLKILIESKVDMDNKKNLLIASVILVSGIGGLML
                     QVNGLQISGVAFSTLLGIILYQVLPEK"
     gene            16839..17774
                     /locus_tag="VHD02_000322"
     CDS             16839..17774
                     /locus_tag="VHD02_000322"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEQ5496960.1"
                     /translation="MSVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVISDH
                     QKDLVANLFFENSTRTHKSFEVAEKKLGLTVLDFNADASAVNKGESLYDTVLTMSALG
                     TDICVIRHPEDDYYKELVESPTITASIVNGGDGSGQHPSQCLLDLLTIYEEFGHFEGL
                     KIAIAGDLTHSRVAKSNMQILKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELD
                     VLMLLRVQHERHDGHQSFSKEGYHQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADS
                     LVEAPKARIVSQMANGVFVRMAIIEAILNGRNKNS"
     gene            17818..18900
                     /gene="carA"
                     /locus_tag="VHD02_000323"
     CDS             17818..18900
                     /gene="carA"
                     /locus_tag="VHD02_000323"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEQ5496961.1"
                     /translation="MTKRLLILEDGTIFEGEPFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLIGNYGINRDDYESISPTCKGVVVSEVSRLASNWRKQMTLDAFLK
                     IKGIPGISGIDTRALTKIIRQHGTMKATMADDGDSIQHLKDQLRATVLPTNTIEQVST
                     KTAYPAPGIGKNIVLVDFGLKHSILREFSKRQCNITVVPFNITAEEVLQLNPDGLMLS
                     NGPGNPEDLPEALDMIRGVQGKIPIFGICMGHQLFSLANGAKTCKMTFGHRGFNHAVR
                     EIATGRIDFTSQNHGYAVERSSLPDTLMVTHEDINDKTVEGVKHRDFPAFSVQFHPDA
                     APGPHDASYLFDEFLEMIDSWRCTSK"
     gene            19146..22322
                     /gene="carB"
                     /locus_tag="VHD02_000324"
     CDS             19146..22322
                     /gene="carB"
                     /locus_tag="VHD02_000324"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEQ5496962.1"
                     /translation="MPKRKDIQKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYKV
                     ILVNSNPATIMTDKEIADKVYIEPLTLEFVNRIIRKERPDAILPTLGGQTGLNMAMAL
                     SKAGILDDLEIELLGTKLSAIDQAEDRDLFKQLMQELDQPIPESTIVKTVDEAVTFAR
                     DIGYPVIVRPAFTLGGTGGGICSSEEELCEITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPTQTLSDIENQMLRDASLKIIRALK
                     IEGGCNVQLALDPYSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     INPITGTTYAMFEPALDYVVAKIPRFPFDKFEHGERQLGTQMKATGEVMAIGRNLEES
                     LLKACRSLEIGVCHNEMTSLSNISDEELVTKVIKAQDDRLFYLSEAIRRGYSIEELES
                     LTKIDLFFLDKLLHIVEIEQELQMHVDHLESLKKAKRYGFSDQKIAEIWQKDESDIRA
                     MRHSHSLYPVYKMVDTCAAEFDAKTPYFYSTYELENESVQSNKESILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQKAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAQALSEAGVTILGTQVEDLDRAEDRDLFEKALKELGIPQP
                     QGQTATNEEEALEAAKKIGFPVLVRPSYVLGGRAMEIVENKEDLREYIRTAVKASPEH
                     PILVDSYIFGKECEVDAISDGKSVLIPGIMEHIERAGVHSGDSMAVYPPQQLSKQIQE
                     TIAEYTKRLAIGLNCIGMMNVQFVIKNEQVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKLILGQTLKDLGYEDGLYPQSQLVHIKAPVFSFTKLAQVDSLLGPEMKSTGEVMGSD
                     TSLEKALYKAFEANNSHLSEFGQIVFTIADDSKAEALSLARRFKAIGYQIMATQGTAA
                     YFAEQGLSACLVGKIGDAANDIPTLVRHGHVQAIVNTVGIKRTADKDGQMIRSSAIEQ
                     GVPLFTALDTAKAMLTVLESRCFNIEAI"
     gene            22528..23799
                     /locus_tag="VHD02_000325"
     CDS             22528..23799
                     /locus_tag="VHD02_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEQ5496963.1"
                     /translation="MSKRGKIKITTKTKLITASVITLVLIITGVVLWKQQQNTLTADI
                     AKEPYSTVSVTEGSIASSTLLSGTVKALSEEYIYFDANKGNDATVTVKVGDQVTQGQQ
                     LVQYNTTTAQSAYDTAVRSLNKIGRQINHLKTYGVPAVSTETNKDEATGEETTTTVQP
                     SAQQNANYKQQLQDLNDAYADAQAEVNKAQIALNDTVVISSVSGTVVEVNNDIDPSSK
                     NSQTLVHVATEGQLQVKGTLTEYDLANVKVGQSVKIKSKVYSNQEWTGKISYVSNYPT
                     ESNAGSTTPAGSTGAGSSTGAAYDYKIDIISPLNQLKQGFTVSVEVVNEAKQALVPLT
                     AVIKKDKKHYVWTYDDATGKAKKVEVTLGNADAQQQEIHKGVAVGDIVIANPDKNIKP
                     DKKLEGVISIGTNTKPEKDSQSKNKKSGVDK"
     gene            23799..24509
                     /locus_tag="VHD02_000326"
     CDS             23799..24509
                     /locus_tag="VHD02_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5496964.1"
                     /translation="MSHEKKQLMQLSNIVKSYQNGDQVLKVLKGINLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDRPTSGDYTLHNTKIEILNDRELAKVRNDEIGFVFQQFFLL
                     AKLTALQNVELPLIYAGVNVSKRREQAKQFLEKVGLGRRIKHLPSELSGGQKQRVAIA
                     RALVNDPSIILADEPTGALDTKTGQQIMELLTELNKEGKTIIMVTHEPEIADFATRKI
                     IIRDGDITTDTTASVVID"
     gene            24521..25741
                     /locus_tag="VHD02_000327"
     CDS             24521..25741
                     /locus_tag="VHD02_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ5496965.1"
                     /translation="MENWKFALSSIWGHKMRSILTMLGIIIGVAAVVIIMGLGNAMKN
                     SVTSTFSSKQKDIQLYFQEKGEEEDLYAGLHTHENNHEVKPEWLEQIVKDIDGIDSYY
                     FTNSATSTISYEKKKVDNASIIGVSKDYFNIKNYDIVAGRTLTDNDYSNFSRIILLDT
                     VLADDLFGEGNYKSALNKVVSLSDKDYLVIGVYKTDQTPVSFDGLSGGAVMANTQVAS
                     EFGTKEIGSIYIHVNDIQNSMNLGNQAADMLTNISHIKDGQYAVPDNSKIVEEINSQF
                     SIMTTVIGSIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRLKILSQFLIES
                     VVLTVLGGLIGLLLAQLSVGALGNAMTLKGACISLDVALIAVLFSASIGVFFGMLPAN
                     KASKLDPIEALRYE"
     gene            25995..27728
                     /locus_tag="VHD02_000328"
     CDS             25995..27728
                     /locus_tag="VHD02_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HEQ5496966.1"
                     /translation="MTFLSDLISLMTKIRLSWVIKAGIFQLLFVTIANIVLSEFFYFI
                     LDVTGQYHLDKDNVVTFLKNPIALALLGAYLFLLAAFIHLEFFALYRIIADQEISFYL
                     FRKQFSYYLRGLWKTFSGYQLLLFLLYILLTIPVLHIGLSSVITQKLYLPEFIVGELS
                     KITSTKYLLYGSLILVFYLNLRLVYFLPLIAINHRTVAQAWRESWQKTKKKHVLLWMK
                     LFAINGLTLVVLSLAISMILIFVDMFNPKGNNIIVQLGALTFTWELIFFTTIFFKLCS
                     AMILKEAIEPQKQYDEPRRSNKAYVVIFIVVTVGFAYQSLERLTFFDTSHSKTVIAHR
                     GLVSAGVENSLEALEGAKKAGSDYVELDLILTKDNHFVVSHDNRLKRLAGVNKTIRNL
                     TLKEVEHLTSHQGHFSGRFVSFDTFYQKAKKLNMPLLIELKPIGTEPGNYVDLFLETY
                     HRLGISKDNKVMSLDLEVIEAIKKKNPSITTGYIIPIQFGFFGDEFVDFYVIEDFSYR
                     SYLSSQAFWNNKEIYVWTINDPKRIEHYLLKPIQGIITDQPALTNQLIKDLKQDNSYF
                     SRLVRIISSLY"
     gene            27855..28127
                     /gene="rpsP"
                     /locus_tag="VHD02_000329"
     CDS             27855..28127
                     /gene="rpsP"
                     /locus_tag="VHD02_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEQ5496967.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQITIKEDRVLEWLSKGAQPSDTVRNILSKAGVMAKFHDQKFSK"
     gene            28137..28376
                     /locus_tag="VHD02_000330"
     CDS             28137..28376
                     /locus_tag="VHD02_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEQ5496968.1"
                     /translation="MDTIENLIIAIVKPLISQPDNLTIKIEDTPDFLEYHLDLDAQDI
                     GRVIGKKGRTITAIRSIVYSVPTLGKKVRLVIDEK"
     gene            29308..32334
                     /locus_tag="VHD02_000331"
     CDS             29308..32334
                     /locus_tag="VHD02_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HEQ5496969.1"
                     /translation="MKKHLKTVALTLTTVSVVTHNQEVFSLVKEPILKQTQASSSISG
                     ADYAESSGKSKLKINETSGPVDDTVTDLFSDKRTTPEKIKDNLAKGPREQELKAVTEN
                     TESEKQITSGSQLEQSKESLSLNKTVPSTSNWEICDFITKGNTLVGLSKSGVEKLSQT
                     DHLVLPSQAADGTQLIQVASFAFTPDKKTAIAEYTSRAGENGEISQLDVDGKEIINEG
                     EVFNSYLLKKVTIPTGYKHIGQDAFVDNKNIAEVNLPESLETISDYAFAHLALKQIDL
                     PDNLKAIGELAFFDNQITGKLSLPRQLMRLAERAFKSNHIKTIEFRGNSLKVIGEASF
                     QDNDLSQLMLPDGLEKIESEAFTGNPGDDHYNNRVVLWTKSGKNPYGLATENTYVNPD
                     KSLWQESPEIDYTKWLEEDFTYQKNSVTGFSNKGLQKVKRNKNLEIPKQHNGVTITEI
                     GDNAFRNVDFQNKTLRKYDLEEVKLPSTIRKIGAFAFQSNNLKSFEASDDLEEIKEGA
                     FMNNRIETLELKDKLVTIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGANNLIFMGS
                     KVKTLGEMAFLSNRLEHLDLSEQKQLTEIPVQAFSDNALKEVLLPASLKTIREEAFKK
                     NHLKQLEVASALSHIAFNALDDNDGDEQFDNKVVVKTHHNSYALADGEHFIVDPDKLS
                     STMVDLEKILKLIEGLDYSTLRQTTQTQFRDMTTAGKALLSKSNLRQGEKQKFLQEAQ
                     FFLGRVDLDKAIAKAEKALVTKKATKNGQLLERSINKAVLAYNNSAIKKANVKRLEKE
                     LDLLTGLVEGKGPLAQATMVQGVYLLNTPLPLPEYYIGLNVYFDKSGKLIYALDMSDT
                     IGKGQKDAYGNPILNVDEDNEGYHALAVATLADYEGLDIKTILNSKLSQLTSIRQVPT
                     AAYHRAGIFQAIQNAAAEAEQLLPKPGTHSEKSSSSESANSKDRALQSNPKTNRGRHS
                     AILPRTGSKGSFVYGILGYTSVALLSLITAIKKKKY"
     gene            32354..32755
                     /locus_tag="VHD02_000332"
     CDS             32354..32755
                     /locus_tag="VHD02_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEQ5496970.1"
                     /translation="MITQEMKDLINNQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGAYYEAAKKWAQGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEINSESN"
     gene            complement(32949..33824)
                     /locus_tag="VHD02_000333"
     CDS             complement(32949..33824)
                     /locus_tag="VHD02_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEQ5496971.1"
                     /translation="MPVSKKVTIIFILNLSFSLIEFIFGTLFFSGAILADAVHDFGDA
                     IAIGISAILERKAVKKESSNFSLGYKRFSLLGALTTNLILISGSLLVMIETIPKLWHP
                     TIVNYDGMFVLAIFAIIINGFASFIIHSNQTKNEEILSLHFLEDILGWLAIIILSLIL
                     KWKPWYILDPLLSIAIASFILSKALPKLVATANIFLDGVPDSIDYCALHHELSQLPHI
                     VSVNQLNVWSMDGIDHRATIHCCLKESTTEKHCKKSIRLICQRYNINSVTVEIDTSLN
                     EHQHHCSSLSGIEVN"
     gene            33960..34481
                     /locus_tag="VHD02_000334"
     CDS             33960..34481
                     /locus_tag="VHD02_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ5496972.1"
                     /translation="MTKDRQIKKTKTAIYSAFIALLQKKEYSKITVRDMITLANVGRS
                     TFYAHYESKEMLLKELCEELFHHLFRQKRNVTFEDYLVHILKHFEQNKDSIATLLLSN
                     DPYFLLRFKNELEHDVYPNLRCKYIDKTTIPEVFLKQFVLSSFIETLKWWLHQRQRMS
                     ANELLKYYLEFIK"
     gene            34696..35214
                     /gene="rimM"
                     /locus_tag="VHD02_000335"
     CDS             34696..35214
                     /gene="rimM"
                     /locus_tag="VHD02_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEQ5496973.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVSDFAEERFKKGSQLALFDDK
                     DRFVQEVTIVSHRKQKHFDIIKFKDMYHINAIEKYKGYTLKVSKDNQGDLQEGEFYYH
                     QIIGMAVYEKDVLIGHVKEILQPGANDVWVVKRQGKRDLLLPYIPPVVLSVDVPNKRV
                     DVELMEGLDDED"
     gene            35204..35923
                     /gene="trmD"
                     /locus_tag="VHD02_000336"
     CDS             35204..35923
                     /gene="trmD"
                     /locus_tag="VHD02_000336"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEQ5496974.1"
                     /translation="MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDTIEQIEAKKPRIILLDPAGKPFTQAYAEELALEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDFVLTGGELAAMTIVDATVRLIPQVLGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMTVPDVLMSGHHERIRLWRLEESLRKTYLRRPDLL
                     ERYDFSEEERKLLDKIKEALG"
     gene            35935..36927
                     /locus_tag="VHD02_000337"
     CDS             35935..36927
                     /locus_tag="VHD02_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEQ5496975.1"
                     /translation="MKDKAYDITIIGGGPIGLFAAFYAGLRGVTVKIIESLSELGGQP
                     AILYPEKMIYDIPAYPSLTGVELTENLIKQLSRFEDRTTICLKEEVLTFDKVKGGFSI
                     RTNKAEHFSKAIIIACGNGAFAPRTLGLESEGNFADHNLFYNVHQLDQFAGQKVVICG
                     GGDSAVDWALALEDIAESVTVVHRRDAFRAHEHSVELLKTSTVNLLTPYVPKALKGIG
                     NLAEKLVIQKVKEDEVLELELDSLIVSFGFSTSNKNLKNWNLDYKRSSITVSPLFQTS
                     QEGIFAIGDAAAYNGKVDLIATGFGEAPTAVNQAINYIYPDRDNRVVHSTSLID"
     gene            37105..38163
                     /locus_tag="VHD02_000338"
     CDS             37105..38163
                     /locus_tag="VHD02_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEQ5496976.1"
                     /translation="MTTTDKETFSSFMNKVLAGTAIAIVVALIPNAILATFLKPLLPN
                     MAAAEFLHIVQVFQFFTPIMAGFLIGQQFKFNPMQQLAVGGAAYIGSGAWAYTEVIQK
                     GVATGTFQLRGIGDLINMMITASLAVLAVKYFGNRFGSLTIILLPITIGTGVGYIGWK
                     FLPYVSYVTTLIGQGINSFTTLQPILMSILIAVAFSLIIVSPISTVAIGLAIGLNGMA
                     AGAASMGIASTAAVLVWATLKVNKSGVPIAIALGAMKMMMPNFLKHPIMAIPMVFTAA
                     ISSLTVPLFNLVGTPASSGFGLVGAVGPIASLAGGNSILIIILAWIIVPFAVAFAAHK
                     VSKDILKLYKEDIFVFEG"
     gene            38176..39099
                     /locus_tag="VHD02_000339"
     CDS             38176..39099
                     /locus_tag="VHD02_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEQ5496977.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNNDVILLDNWEDHINAIKENGL
                     IVTGDVEETVKLPIMKPTEATQEADLIILFTKAMQLPQMLQDIKGIIGKETKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLEGPGRAHLQGVGALNLQSMDPNNQDAGHQV
                     ADLLNKANLNATYDENVVPNIWRKACVNGTMNSTCALLDCTIGELFASEDGLKMVKEI
                     IHEFVIVGQAEGVELNEEEITQYVMDTSVKAAHHYPSMHQDLVQNHRLTEIDFINGAV
                     NTKGEKLGINTPYCRMITELVHAKEAVLNIQ"
     gene            39355..40068
                     /locus_tag="VHD02_000340"
     CDS             39355..40068
                     /locus_tag="VHD02_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ5496978.1"
                     /translation="MAKITEENYVSLEDLMQLLNSSESTIRRDLGELEQEGRLHRVHG
                     GAELFHSLQEELSNQEKSVKNSHIKKAIAQRASQLIYDNDVIFIDAGTTTEFLLPFLQ
                     AKNLTVVTNSIHHAARLVELSIETIIVGGYVKQTTDASIGNVALEQIRQMNFDKAFLG
                     MNGVDDSYLTTPDMEEAVIKKAVLSNAKLAYILVDGTKIGQVSFVKVAPINDVTIITL
                     GGSASILKQIKEKAKVIEL"
     gene            40065..40976
                     /gene="pfkB"
                     /locus_tag="VHD02_000341"
     CDS             40065..40976
                     /gene="pfkB"
                     /locus_tag="VHD02_000341"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEQ5496979.1"
                     /translation="MIYTVTLNPSIDFIVRIDQINLGSVNRMASDDKFAGGKGINVSR
                     ILQRLDIASTATGFLGGFTGRFIEESLSAEGVKTDFVKGDQDTRINVKIKSQEETELN
                     GQGPIISREQLEELKTKLSQLTAEDTVVFAGSAPANLGNAVYKELLPLVRQSGAQVVC
                     DFEGQTLIDALAYNPLLVKPNNHELEAIFGTILTSLDDVETYARRLLEMGAQNVIISM
                     AGDGALLVTKEATYFAKPIKGEVKNSVGAGDSMVAGFTGEFVKSQNPIEALKWGVACG
                     TATAFSDDLATIAFIKETYHKVEVEKR"
     gene            40973..42919
                     /locus_tag="VHD02_000342"
     CDS             40973..42919
                     /locus_tag="VHD02_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ5496980.1"
                     /translation="MKIQDLLRKDIMILDLQAISKEVAIDEMITKLVEKDIVHDFDVF
                     KKSIMTREEQTSTGLGDGIAMPHSKNIVVDKPAVLFAKSNKGVDYKALDGQPTDLFFM
                     IAAPQGANDTHLAALAELSQYLLKDGFADKLRAAATPEAVIAVFDEASTAKEEVVAPT
                     SGQDFIVAVTACPTGIAHTYMAEEALKKQAAEMGVAIKVETNGASGVANRLTAEDIQR
                     AKGVIVAADKAVEMDRFDGKQFIARPVADGIKKSQELISLILNNEGHTYHAKNGKSET
                     AVSSEKTSLGGAFYKHLMGGVSQMLPFVIGGGIMIALAFLLDNMLGVPKDQLGNLGSY
                     HEVAAIFMKIGGAAFSFMLPVLAGYIAYSIAEKPGLVAGFVAGTIASNGLAFGKVPFA
                     AGGEVSLGLTGVPSGFLGALVGGFLAGGVILALRKLLAGLPRSLEGVKSILLYPLLGV
                     LVTGFLMLFVNIPMAAINTALNDFLQGLSGSSAVLMGLLVGGMMAVDMGGPVNKAAYV
                     FGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFTKEERESGLTNIVMG
                     LSFITEGAIPFGAADPARAIPSFIAGSALTGALVGLAGIKLMAPHGGIFVIALTSNPI
                     LYLVFVVIGALVSGILFGALRKKA"
     gene            43012..43611
                     /locus_tag="VHD02_000343"
     CDS             43012..43611
                     /locus_tag="VHD02_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEQ5496981.1"
                     /translation="MKVKKRRRRARSSVNRLVLGLVLLNLIVSMWTLKLGNQRLAPYA
                     DHETLTFVRKISHAAQSVAQKKQLYSSVMMAQAILESNNGKSQLSQKPYYNFFGIKGS
                     YKERSVIFPTLEDDGQGNLYQIDAAFRSYGSLTACFLDYARVLNDPLYDKTHKKFWSH
                     YQDATATLTGTYATDTTYHTKLNELIEWYQLTNFDGLMK"
     gene            43763..44470
                     /locus_tag="VHD02_000344"
     CDS             43763..44470
                     /locus_tag="VHD02_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEQ5496982.1"
                     /translation="MTKKKGKLVLISLFVLAACLGAYSAMRQSHKTSNVSAETIASSS
                     TRHFIDEIGPTASTIGQERDLYASVMIAQAILESSNGKSSLSQAPYYNFFGIKGAYNG
                     SSVTMSTWEDDGNGNTYTIDQAFRAYPSIADSLNDYADLLSSSTYIGARKSNTLSYQD
                     ATAALTGLYATDTSYNLKLNNIIATYGLTAYDVANSSAQETGLATSGYVWNEYRRNYT
                     DAETLAVDEAWAKRMTY"
     gene            complement(44527..44838)
                     /locus_tag="VHD02_000345"
                     /pseudo
     CDS             complement(44527..44838)
                     /locus_tag="VHD02_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(45042..45407)
                     /locus_tag="VHD02_000346"
     CDS             complement(45042..45407)
                     /locus_tag="VHD02_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5496983.1"
                     /translation="MPSTDDGILRTNTIAGNFFIDGQQQKTLINPIKIDKDIVTIQEF
                     DFKIRKFLMESKEIHLTKSPYIRGSLEIHSKNRKHEKINLYDAKPNSTRSDVLKKYKD
                     NKTINMKDFSHFDIYLWTK"
     gene            complement(45427..46452)
                     /locus_tag="VHD02_000347"
     CDS             complement(45427..46452)
                     /locus_tag="VHD02_000347"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922160.1"
                     /note="This family includes IgM or IgG-cleaving cysteine
                     proteases; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IdeS/Mac family cysteine endopeptidase"
                     /protein_id="HEQ5496984.1"
                     /translation="MRKRCYSTSAAVLAAVTLFALSVDRGVIADSFSANQEIRYSEVT
                     PYHVTSVWTKGVTPPAKFTQGEDVFHAPYVANQGWYDITKTFNGKDDLLCGAATAGNM
                     LHWWFDQNKEKIEAYLKKHPDKQKIMFGDQELLDVRKVINTKGDQTNSELFNYFRDKA
                     FPGLSARRIGVMPDLVLDMFINGYYLNVYKTQTTDVNRTYQEKDRRGGIFDAVFTRGD
                     QSKLLTSRHDFKEKNLKEISDLIKKELTEGKALGLSHTYANVRINHVINLWGADFDSN
                     GNLKAIYVTDSDSNASIGMKKYFVGVNSAGKVAISAKEIKEDNIGAQVLGLFTLSTGQ
                     DSWNQTN"
     gene            46952..47202
                     /locus_tag="VHD02_000348"
                     /pseudo
     CDS             46952..47202
                     /locus_tag="VHD02_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051153.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            47356..47736
                     /locus_tag="VHD02_000349"
     CDS             47356..47736
                     /locus_tag="VHD02_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEQ5496985.1"
                     /translation="MQLVREKEFVNQYHYDARNLEWEKENGTPETNFEVTFQLIDKDE
                     QQKETVIVSVLQFVIVKEEFVISGVISQMVRILDRLVDKPSEFTQEEVESLAAPLLDM
                     VKRLTYEVTEIALDRPGIHLEFKN"
     gene            47736..48584
                     /locus_tag="VHD02_000350"
     CDS             47736..48584
                     /locus_tag="VHD02_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ5496986.1"
                     /translation="MKLAVITDSTATLPTDLKQDKAIFSLDIPVIIDDETYFEGRNLS
                     IDDFYQKMADSQNLPKTSQPSLSELDNLLGLLSSKGYTHVIGLFLAGGISGFWQNIQF
                     LAEEHPEIEMAFPDSKITSAPLGSMVKNVLDWSRQGMTFQAILNKLQEQIDGTTAFIM
                     VDDLNHLVKGGRLSNGSALLGNLLSIKPILRFDEEGKIVVYEKVRTEKKAMKRLVEIL
                     NDLIADGQYNVSIIHSKAQDKADYLKRLLQDSGYQYDIEEVHFGAVIATHLGEGAIAF
                     GVTPRL"
     gene            48709..49917
                     /locus_tag="VHD02_000351"
     CDS             48709..49917
                     /locus_tag="VHD02_000351"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEQ5496987.1"
                     /translation="MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIH
                     DVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENGGEYEITTFRTEDVYVDYRRPSQ
                     VSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLEQKRLRAVGKAEERFEEDA
                     LRIMRGFRFAASLDFDIEAATFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGIS
                     AMIACQAYDYLPGLKQQEAGLNHLIVSLKDNFTFSDHHQAWAYVMISLAIEDPKSFLK
                     AWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKEMASLVEGLRKAQSLSVDMDH
                     IEALDQALAIHNKYDIVLNGSHLIKDFGMKPGPQLGLMLEKVELAIVEGRLDNDFTTI
                     EAFVREELAT"
     gene            49914..51791
                     /locus_tag="VHD02_000352"
     CDS             49914..51791
                     /locus_tag="VHD02_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEQ5496988.1"
                     /translation="MSHFLVEKLTKTVGDKTVFQDISFIIHDFDRIGIIGVNGTGKTT
                     LLDVLSGRLGFDGDHSPFSKANDYKIAYLTQDPEFNDASSVLDTVLSSDVKAIQLIRQ
                     YELLMANYTEDKQETLESLMSEMDRLDAWSIESDVKTVLSKLGITDLEQKVGDLSGGM
                     RRRVQLAQVLLGAADLLLLDEPTNHLDIDTIAWLTTYLKTAKKTVLFITHDRYFLDHV
                     ATRIFELDKAGLTEYQGNYQDYVRLKAEQDERDATNLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFTDLKKEVHQDSSADKLEMAFETSRIGKKVIHFEDLSFAYGDRQLIKD
                     FNLIIQNKDRIGIVGDNGVGKSTLLNIINGDLKPTSGKLDIGDTIRVGYFSQQLKDMD
                     ETKRVINYLQEVADEVKTSVGTTSISELLEQFLFPRSSHGTLIAKLSGGEKKRLYLLK
                     LLIEKPNVLLLDEPTNDLDIATLTVLENFLANFAGPVITVSHDRYFLDKVATKILAFE
                     EGDIRIFYGNYSDYLDEKAFEKETVEADLAKAIVTEEMPLPPKEEKKRMSYLEKQEWA
                     QIEDKIATIEANIEEIENQMLTVVSDYGQLAQLQKELDQRNNDLLLAYERFEYLSGLD
                     I"
     gene            complement(51889..51966)
                     /locus_tag="VHD02_000353"
                     /pseudo
     CDS             complement(51889..51966)
                     /locus_tag="VHD02_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            52681..53091
                     /locus_tag="VHD02_000354"
     CDS             52681..53091
                     /locus_tag="VHD02_000354"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEQ5496989.1"
                     /translation="MIREIITDHFLLQQKAQVAKKEDLWIGQDLQDTLAFYRQECLGM
                     AANMIGEQKRIVIVSMGFIDLVMFNPVMVSKKGIYQTKESCLSLSGYRKTQRYDKITV
                     EYLDHNWRPKRLNLTGLTAQICQHELDHLEGILI"
     gene            complement(53173..55146)
                     /locus_tag="VHD02_000355"
     CDS             complement(53173..55146)
                     /locus_tag="VHD02_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEQ5496990.1"
                     /translation="MLTSFTLLVTDVQADQVDVQFLGVNDFHGALDNTGTAYTPSGKI
                     PNAGTAAQLGAYMDDAEIDFKQANQDGTSIRVQAGDMVGASPANSALLQDEPTVKVFN
                     KMKFEYGTLGNHEFDEGLDEFNRIMTGQAPDPESTINDITKQYEHEASHQTIVIANVI
                     DKKTNDIPYGWKPYAIKDIAINDKIVKIGFIGVVTTEIPNLVLKQNYEHYQFLDVAET
                     IAKYAKELQEQHVHAIVVLAHIPATSKDGVVDHEMATVMEKVNQIYPEHSIDIIFAGH
                     NHQYTNGTIGKTRIVQALSQGKAYADVRGTLDTDTNDFIKTPSANVVAVAPGIKTENS
                     DIKAIINHANDIVKTVTERKIGTATNSSTISKTENIDKESPVGNLVTTAQLTIAKKTF
                     PTVDFAMTNNGGIRSDLVVKNDRTITWGAAQAVQPFGNILQVIQMTGQHIYDVLNQQY
                     DENQTYFLQMSGLTYTYTDNDPKNSDTPFKIVKVYKDNGEEINLTTTYTVVVNDFLYG
                     GGDGFSAFKKAKLVGAINTDTEAFITYITNLQASGKTVNATIKGVKNYVTSNLERSTK
                     INSAGKHSIISKVFRNRDGNIVSSEVISDLLTSTENTNNSFGKKETTTNKNTISNSTL
                     PITGDNYKMSPIMTILALISLGGLNAFIKKRKS"
BASE COUNT        17480 a         9069 c        11408 g        17190 t
ORIGIN      
        1 ctgacagcag gggtacaatt attagatgaa aaagattttt ctaatataaa ggaaaaaaat
       61 cagttggttc aactggtgac caagtccaat cgtcccattg gaacagcata tatctctaag
      121 caaaataaag gaattggttg gtatctagga ccagaaaaaa tcgacttgtc tatctcttat
      181 tttgttagtc tattttcagt tgctaaggcc aaaagacaag attttgctca atcggacgag
      241 accaatgctt atcgcctttt taatcaagaa ggagacggtt ttggtggagt aacaattgat
      301 ctttataaag attttgcagt cttttcatgg tacaatgcct ttgtctatga caaaaaagaa
      361 atgattatgg aggcttttca acaggttttt cctgaagtca aaggagctta tgaaaagtgt
      421 cgctttaaag gtccagatac tgaaactgct catctgtatg gtgagctggc acaagaaacc
      481 ttttcgattt tagaaaatgg catagcctat caagtcttct tgaatgaggg cttaatgaca
      541 ggtatttttc tagaccagca tgatgttaga agagcactag tagatggttt agctatgggc
      601 aagtcccttc ttaacctatt ttcctatacg gctgcctttt ctgtagcagc tgctatgggt
      661 ggtgctatcg agaccacttc tgtggacttg gctaaacgat cgagagaatt atcactagct
      721 cattttgagc ataaccagtt aaacttagcc tcacatcatt ttgtggtgat ggatgttttt
      781 gaatacttca aatatgccaa acggaaaaaa ttaatctttg atgtgattgt gattgaccca
      841 ccaagctttg ctagaaataa aaaacaaact ttctctgtga gcagggacta ccacaaatta
      901 atcacagagg ccttggacat attgagtcct aagggaacta ttatagcttc aacaaatgct
      961 gctaatatga ctgttagcca gttcaaaaag caaattatca aaggatttgg aagtcgtcgt
     1021 cctgagtcta tgactctcca gcagcttccg agtgacttta ctatcaataa agcagatgaa
     1081 agaagtaact atttaaaggt atttactata aaggtacgag aatgagaatt gtagcaccag
     1141 tcatgccaag acattttgac gaggctcaag ccattgatat ttctaaatat gaagatgtta
     1201 atttaattga atggcgagca gactttcttc ccaaggatga gattgtcgca gtagcaccag
     1261 ctatttttga aaaatttgcc ggaaaagaaa ttatctttac ccttcggacc gttcaagaag
     1321 gggggaatat taccctttca agtcaggagt atgttgatat tatcaaagaa atcaatgcta
     1381 tttataatcc agactatatt gactttgagt attttacgca caagtcagta tttcaagaaa
     1441 tgcttgattt tccaaatctt attttgtctt atcacaactt tgaagagact cctgaaaatt
     1501 taatggaagc tttttcagaa atgaccaagt tggcaccgcg tgtggttaaa attgctgtga
     1561 tgccccagag tgaacaagat gttctagatt tgatgaacta taccagaggc tttaagacgc
     1621 tcaatcctga gcaagaattt gcaactatat ctatggggaa attaggaaga ttgtctcgtt
     1681 ttgcgggaga tgtcattggc tcttcctgga catacgtttc attagatcat gttagcggtc
     1741 caggtcaagt gactctcaat gatatgaaac gtattattga ggtcttagaa atggatatct
     1801 ctaactagtg aatggtctaa aaatattatt tctacaatag ttaagaaatg atgtttttag
     1861 attaatgttt ttaaaataag tggaaccaaa aaggagaaaa attgcgttat ttaacagcag
     1921 gagaatctca tgggccatcc ttaacagcta ttattgaagg tataccagca ggattaactc
     1981 ttcatccagc ggatattgat cacgaattac agcgacgtca aggaggttat ggccgtggtg
     2041 ccagaatgtc tattgagact gatcgggttc aaatatcctc aggagttcgt catggcaaaa
     2101 caacaggggc ccccattacc ttaacggtaa ttaataagga ccaccaaaaa tggctagatg
     2161 tcatggctgt aggagacatt gaagagactc tcaagttaaa gcgtcgtgtt aagcatccta
     2221 gaccaggaca tgcggatttg gtaggaggta ttaaatatca ttttaacgat ttaagagatg
     2281 ctttagagcg ttcctcggct agagaaacaa cgatgagagt ggctgtaggc gctgttgcta
     2341 aacgtatctt agctgaactt ggaattgata tgttacatca tatcttaata tttggtggta
     2401 taacgatcac aataccttct aagctctctt ttagagagtt gcaagaaagg gctctccatt
     2461 cagaattatc tattgttaat ccaaagcaag aagaagaaat taaaacgtat attgataaga
     2521 taaaaaaaga aggggatact attggtggta ttatcgaaac gatagttcaa ggggtgccag
     2581 caggtcttgg atcttatgtc caatgggata aaaaattaga tgccaagctg gctcaagcag
     2641 ttctttccat taatgctttt aaaggggtgg aatttggagt tgggtttgat atgggattcc
     2701 aaaagggctc tcaggtaatg gatgaaatca cctggacacc tacccaaggc tatggtcgtc
     2761 aaacaaacca cttaggtggc tttgaaggtg gcatgaccac aggacaacct cttgttgtta
     2821 aaggggttat gaagcccatt cctactttgt ataaaccact catgtcggta gatattgata
     2881 gtcatgaacc ttataaagca acggtggaac gctctgatcc gacggcctta ccagctgcag
     2941 gggttatcat ggaaaatgtg gtggcgacgg tgcttgccaa agagatttta gagacttttt
     3001 cttctactac catgtctgaa ttgcaaaaag ctttttcgga ctatcgtgcc tacgtcaagc
     3061 aattttagat agcgagatac tggcttttat ggtatcatac aggtattaat gaacaaaagg
     3121 agactgtcat gtcacaagaa atttatgact atgctaatca acttgaaaga gcagttcgtg
     3181 ctctgccaga ataccaaaaa gtgttagaag taaaagaggc cattcaagca gacgctagtg
     3241 ctagcgaact gtttgatgag tttgtcgcta tgcaagaaaa aatccaaggg atgatgcaat
     3301 caggccaaat gccaacggct gaagaacaaa caagcattca agaacttagc caaaagattg
     3361 aagctaatga ccagctaaaa gcttattttg aagcgcagca ggccttatct gtttacatga
     3421 gtgatattga gcgtattgta tttgctccgt tgaaagattt agtcaagtaa gccaattaag
     3481 ataggaaaaa aacagattgc tggcagctcg tcaggatctg ttttttagtg tttattttct
     3541 ttgtatcaga aagttgagcc tctttcttat ttgtgatata gtcaaataaa gtgaccaaaa
     3601 tagcaggcca gtagtggctt taatgaaggc aagttgtctg ctaactgact atgctaaaac
     3661 atcagtatta gaaagtaaaa ggtgataaaa atggttattc cttatgatta tattgttatt
     3721 ggtggaggga gtgcaggtat tgcttctgcg aacagggcag ccatgcatgg ggccaaagta
     3781 ttgttagctg aaggtaaaaa gattggggga acctgtgtta acctaggctg cgttcctaaa
     3841 aaagtcatgt ggtatggcgc tcaagtagca gatattttag ggacttacgc taaggattat
     3901 ggatttgact ttaaagagaa agcatttgat tttaaacaat tgaaagcaaa tcgtcaagct
     3961 tatattgatc ggattcatgc ttcttatgag cgaggctttg agcagaatgg tgttgaccgt
     4021 atttatgatt atgctgtttt caaagatgct catacggtag aaattgcagg gcagctttat
     4081 accgcaccgc atattttgat tgccacagga ggacatcctg tttttccaga tattgagggg
     4141 gcacaatacg gtatcagctc ggacggcttt tttgccttag atgaggtacc aaaacgtacg
     4201 gctgtggttg gggcaggcta tattgctgtg gaacttgccg gtgttttgca tgctttgggg
     4261 tcgaagacgg atttgtttat ccgtcacgat cgacctttaa gaagttttga caaagccatt
     4321 gtggatgttt tagttgagga aatggcagtt ggtggtcctt gcttacaccc tcattcagaa
     4381 gtggcaaagg ttgtcaaaaa tactgatgag tccttgaccc tttacctcaa agatggccaa
     4441 gaagttgagg ttgaccagct catttgggct atcggtcgca agcctaatct agaaggtttt
     4501 ggtcttgaca aaactggagt gacgttaaat gataaaggtt atattgagac agatgcctat
     4561 gaaaacacgt ctgtcaaagg tatttatgcc gtgggagatg ttaatgggaa actggcttta
     4621 actccagttg ctgtggcagc tggacgtcgc ctatcagagc gtcttttcaa tggcaaaata
     4681 gacgagaaat tggactacca aaacgttgct actgtgattt ttagtcaccc tgttattggc
     4741 tctgttggct tgtcagaaga agcagcagtc aaacagtatg ggcaagaagc agttaaaaca
     4801 tatcaatcac gttttacgtc tatgtttact gctatcacta accatcgcca accttgtctg
     4861 atgaagttgg taacagtcgg agataccgaa aaaatagttg gtcttcacgg aatcggctat
     4921 ggggttgatg aaatgattca gggctttgca gtagccatca aaatgggagc aaccaaggcg
     4981 gattttgata acaccgttgc tattcaccca actggctcag aagaatttgt caccatgcgc
     5041 taaaaaatgt agcctaaggc aataaaaaat gatgttaaca gtttttgagg aaagtcttct
     5101 aaactgctag catcgttttt tgttaggtga ttaggggaat atgtttttaa agatatttgc
     5161 gttgctcaga caatattgcc ttacctcgga gataaaataa agtgaccctg taacaacaaa
     5221 gagttcatca gatgttacgg aaccttgtaa ccagtctctg taatctttta cctgtgggta
     5281 tttatcagga taatcctcta aaagatttgc ttcagggaaa tcaaaagtcg tgacagatac
     5341 ttcaaaagta tctagttgag ccagcaagtc ggctaaaggc ttacgtttga gacctgcaaa
     5401 gagaatatga ataggacgtt tgggaaattc ttcttgcagt agagacttta atttagcaat
     5461 ggaatctgga ttgtgagcgc cgtctaatag aagattaggt tgaataaatt cggaccggcc
     5521 aggccaagtt gttgcctcaa ttcctttttg aatcgttttt ggagatattt taggaaagga
     5581 ttttctaagt aattgagctg tcataatggc taaacaggca ttcgcttttt gatgttgacc
     5641 taatagtttg agacggagtt tggttaaatt caagttatcg tgttgaaagg aaaacgtctt
     5701 ttctgaagga ttgagcaaaa aatcttttcc taattgatat aagcgtgaac cggctttttg
     5761 ggtaacttgt tgaaagactt gactagcctc ctgctcaagt tgaccgataa taacgggttc
     5821 tttagctttg ataacttctg ctttttggtg agcaatttct gctaggctat aacctaaacg
     5881 ttcttggtga tcaaaactaa tagaagggca tacgacggcc agagcatgaa aaacattggt
     5941 tgcgtcggtt ttcccaccaa tccctgcctc aataatggca atatcaacag gtttaagatt
     6001 ggcaaagtag caaaacatta tgagcgtcac taactcaaac tcggtaggtc tatcccaatt
     6061 tgtttcaagg gacattttag tgagaatggg tctaatcacg ctcacacatt gcgccaattc
     6121 taggtctgaa atgggctggc cattaaggca aatccgatcg tgaaaagaga taataaaagg
     6181 agatgtaaag gttccgacct gataaccaga agttgacagg atatgctgta agtaggctgt
     6241 cacagagccc ttgccgttag ttccaacaac gtgaatagca gggaatttgt cttgtggccg
     6301 gcctagtgcc tcaagtaacc aagccattct ggccaaatcg gtcttgcgac cattagcctt
     6361 aaaggtatga atccaggaaa tgctatcata ggtagtcatt actatttctt gtccttctaa
     6421 caatgctttt aaggtttcgt aggaagacca gtattagcat cttcagctcc agttgcatta
     6481 ggaagttgcg tcccttcagt ggttgatggt gtctcaggag ctgtaggagt agtctcgttg
     6541 tgagaaggtg cggtagaaga tgacggaaga gttgtcttat tatcatctgt ggtttctgaa
     6601 gtacctgtat ggttttgatt actctgagaa gactcttttt caataatctt gacaatggta
     6661 ttactcgatt ctttgggtgt atcattagag gtcttatctg gtacaggctg cagtgctctt
     6721 gaaatggtaa tggttgccac gatgatactc aaaatactac caataagtgc aatggatttt
     6781 tggaaagctg taaaacctgt tttaatatgc tcggtaggga cacctcctga tttacgaggt
     6841 ttttgttgac gggaataacg tgatgacata tttttctcct ttaaaatcat cgtaatgcca
     6901 agattaagga ggagtaaaca gaagtcctag ctcctcttta acacttcatc tatcagtata
     6961 atctagaaaa aaagtgaagt caacggttta ccttatgtct aggacaagga agtcctttgg
     7021 gcttattgta atacgaatat tcttgatttc attttagcta ttttgtgata gaataaagta
     7081 ttgagtaaaa atgaaggtga cacatgattt attttgataa tgcagccaca accatacctt
     7141 atggagaggc tcttaaaact taccaagaag tagcaacaaa aatatatggc aatccctcaa
     7201 gtctgcacca attagggaca aatgcgtctc gcattttaga agcttcccgt aaacagattg
     7261 ctggtttgtt gggtgttaag tcagaagaaa tcttttttac gtctggtggg actgaaagtg
     7321 ctaattgggc tattaaggga attgcttttg aaaaaactgc ttttggcaaa catattatta
     7381 tctcagccat tgaacaccct gccgtgagtg aaagtgtcaa atggcttctc acacaaggat
     7441 tcgaagtttc ttatgctcca gtgacaacac agggagttgt tgatgtgaat gctttagctg
     7501 agctgattag accagacacc atcttaatct cgattatggc tgttaataat gaaatgggag
     7561 ctatccaacc gattagagcc atttcaaacc tattggctaa ccaaccaacg atcacctttc
     7621 acgttgatgc tgttcaagcc attggtaaaa taccactctg tgattatatg actaatcgtg
     7681 tggatttggc ttctttttca gggcacaaat ttcatagtgt tcgtggtgtt ggcttccttt
     7741 ataaaaaagc aggcaagcga ctcaattctc tcttgtcagg cggcggccaa gagcaggagt
     7801 tacgttcaac cactgaaaat gttgcaggaa ttgctagcat ggccaaagca ttaagaatag
     7861 tcactgaaaa acaagtgagc gtcttaccta aactaacagc gatgcgagat gtgatttaca
     7921 aagctttgtc cgcttaccca gatgtgacag tattttcagc tcaagaaggc tttgcgccca
     7981 atattttgac ctttgggatt agaggggttc gaggagaagt tattgtccac gcctttgaaa
     8041 aatatgagat ttatatttcg accacaagtg catgttcgtc caaagcaggt aagccagcag
     8101 gaagcctagt ggctatgggg attcctgtaa aaactgccca gacagccgtt cgtattagtc
     8161 tagacgatga caacgatatg ggacaagtag agcagttttt gaccatattc aaacagattt
     8221 atgataaaac acagaaagtt aggtaagatt aggtaagatg gactattcag aaattatggt
     8281 tcgccatggt gaactgtcaa ctaaagggaa gaatcgcatg cgattcatta acaaacttaa
     8341 aaacaatatt caagacgtac tggctccttt cccagccatt gctgtccgtt cagatcgtga
     8401 taggacccat gtgtctctaa atggcacaga ttatcagcct attgtagaag ctttaaagct
     8461 ggtgtttggt gttcaagctc tatctcctgt ttataaactt gaaaaaagtg tccctctttt
     8521 ggtaacagct gttcaagata tcatgacttc tctttatcgt gatggactga cgtttaagat
     8581 tgccactaag cgtagtgatc acgcgtttga attggatagt cgtgagctta acagtcttct
     8641 tggtggagct gtttttgagg ttttaccaaa tatccaagcc cagatgaaac atcccgatgt
     8701 gactctcaaa gtcgaaattc gtgatgaagc agcttacatt tcttatgaag agattaaggg
     8761 agcaggcggg ttaccagtag gaacgtcagg gaaagggatg ctgatgttat cgggcggtat
     8821 tgattcacca gtagctgggt atttagccct taaacgtggc ctcgatattg aagttgttca
     8881 ttttgctagc cctccgtaca ccagtccagg tgccttggct aaagcccaag accttactcg
     8941 tcggttaact cgttttggtg gaaatatcca atttatcgag gttcctttta cagaaattca
     9001 agaagaaatt aaaaataagg caccagaggc ttaccttatg acattaaccc gccgctttat
     9061 gatgcgcatc acagatgcca ttcgtgagca acgtaagggt cttgttattg ttaacggtga
     9121 gagcctaggt caggttgcta gtcaaaccct tgaaagcatg caggccatta atgcggtaac
     9181 atcaacccca attattagac cagtcgtgac aatggataaa cttgaaatta tcgagatggc
     9241 tcaggcgatt gatacttttg acatttctat tcaaccgttc gaagattgct gtactatttt
     9301 tgcaccagac cgtcctaaaa ccaaccctaa actaggtaat gctgaaaaat atgaagaacg
     9361 ctttgatatt gatggtcttg tccagcgtgc tgtgtcagga attgttgtca cagaaatcac
     9421 acctgaaatc gtcaatgatg aggttgaaaa tctcattgat gctttacttt aaaagaaaag
     9481 atcaagtgta aatcttttag tagtcagtaa tgatactatt gaaaagcctc tataagggct
     9541 tttttgctgc aatgaaggag gaaaattttg aggcaagatg ttataataga gaaaaaggag
     9601 ggtgtgtctt gattaaaact atgcaataca aaaaaacgat ggcaacagtt gttgctctta
     9661 tcgtcgcact tttgctattt ggcttgattt atgatttact tggggttcaa aaaaataagc
     9721 tagcagctca aaaatctgct cagccaaagg tcaaaacagc gcgtgttgta gctaatggcg
     9781 acattttaat tcatgatatt ctctacatga gtgctagaaa agctgacgat acctatgatt
     9841 tcactcctta ttttgaatat gttaaggact ggattagcgg tgctgactta gctattggag
     9901 attatgaagg gaccattagc ccagattatc ccttagcagg ctatccctta ttcaatgctc
     9961 cagaagagat tgcaggtgct ctgaaaaata ctggctatga tgtagtcgat ttagcccata
    10021 atcatatctt agattcccaa ttagacggtg cccttaacac taaaaaggtt tttcatcaat
    10081 taggtataga cagtattggc atctatgaca aggatcgttc aaaagaatcc ttcttaatta
    10141 aaaatgtcaa tggtatcaaa attgcaattt taggttattc ttatggttat aacggcatgg
    10201 aggccacact tagccaagaa gactatgaga aacacatgtc tgatttagat gaagctaaga
    10261 taaaaaaaga acttcagcta gctgaaaaaa aggctgacgt gaccattgtc atgccacaaa
    10321 tgggaacaga atatgcctta gaaccgacag cagagcaaaa agaactttac cacaaaatga
    10381 ttgattgggg agctgatgtc gtcctaggag gccatccgca tgttattgaa ccttcagaga
    10441 cagttataaa aggtaggcaa aagaaattca ttatttactc tatgggaaat tttatttcaa
    10501 atcagcggct tgaaaccgta gatgatatct ggactgagcg tggcctattg atggacctta
    10561 cttttgagaa aaaagataac aaaacaaaaa ttaaaacagt tgaggcccat ccaaccatgg
    10621 ttttagccaa aggtaaaggc attgtgggta aagaaggttt tgaactatac aattatcgaa
    10681 caatggtttt agaagatttt atcaaaggtg gaaaatacca cgacaagatt gatgaggaaa
    10741 ccaaagcaaa agtagcactt gcctatcagg aaattaatga tttagttaac ctgaaatggt
    10801 aatgtttggt ggtttagcat ctaacgataa aaaatagaga ttaaacttaa tttttccttg
    10861 ataaaaagca gttatggtgc taaaatagta acgttgacta aatgcacatc ctgtgcaacc
    10921 gcacgaaaat cgttgtcaag tagaccaagt ctcacgattc caggcgagtc ttcacagagg
    10981 gaaacctcaa ctaaaaattt tataggaggt gcatcatgag cacatacgca atcatcaaaa
    11041 ctggtggaaa acaagttaaa gttgaagtag gtcaagcaat ctacgttgag aaaattgacg
    11101 ctgaagctgg cgcagaagtc acttttaacg aagttgttct tgtaggtggt gacaaaactg
    11161 tagttggtac tccagttgtt gaaggagcta ctgtcgttgg aactgttgaa aaacaaggaa
    11221 aacaaaagaa agttgtaact ttcaaataca aacctaaaaa aggtagccac cgtaaacaag
    11281 gtcatcgtca accttacact aaagttgtca tcaacgcaat caacgcttaa tttactagct
    11341 tatgattaaa gcaattttta ctcgccaaaa aaatggtcaa ctgagcagtg ttacactgac
    11401 aggtcatgct ggtagtggta aacatggctt tgatattgtt tgtgcctctg ttagtactct
    11461 agccattaac tttgttaatt ctcttgaggt gttagcagat tgccaagcat tagtagatct
    11521 taatgatgtt gaaggtgggt atatggctat tacaatacca ccacatgaca acaaggagga
    11581 agttcaatta ttatttgaat cttttcttct aggaatgact agtttggcga aggattcttc
    11641 gaaatttgtc aacactcagg tcatctaaat ttaatttaag agaggaaaca acattatgtt
    11701 aaaaatgaat cttgctaact tgcaactttt cgcccacaaa aaaggtggag gttctacatc
    11761 aaacggacgt gactcacaag ctaaacgcct tggtgctaaa gctgctgacg gtcaaactgt
    11821 ttcaggtgga tcaatccttt accgtcaacg tggaactcat atctacccag gtgtaaacgt
    11881 aggccgtggt ggagatgaca cactttttgc taaagttgaa ggtgttgtac gtttcgaacg
    11941 taaaggacgc gataaaaaac aagtttctgt ttacccagta gctaaataat tgtttaacat
    12001 cgcattatat aaggctttcc aggtgtctgg aaagccgatt tttttatttt gctactcatt
    12061 tgctacccat attctaaaag ccgatgtagg aagagagctt gtcagcagtt ttatcttttg
    12121 aggatttagt aacatgagcg tagatattca tggtagtttg gatagtttca cgaccaagtg
    12181 tattggattt tcttctatcc tactaaagat tgtagttgct tatttagagc aatagttcta
    12241 attgtaaaaa gtcagcataa taaaacaata tcataagatg ttgttataac gggctttatg
    12301 gtataattaa ggatactcat tttaggaagg aaagcaatga atattcaaca attacgctat
    12361 gtggtagcca tcgctaataa tggaactttt cgagaagctg cctctaaatt gtttgtcagt
    12421 cagcctagct tatcagtttc tatcaaagat ttagaggcgg aattagggtt tcaaattttt
    12481 aatcggacta cttcaggaac agtcttgaca agtcaaggac tagtttttta tgagaaagct
    12541 ttagaagtag tcaaatcttt tgattctttt gaaaaaacct tttctcaagc tgatctggat
    12601 caaaatgaat tttctattgc tagccaacat tatgactttt tgccacctct tattacagct
    12661 ttttcgcaac aatacgatgg tcatcgtgtc tttcgtatct ttgaatcaac aactattcaa
    12721 attttagatg aagttgctca aggaaacagt gaaattggta ttatttactt gaatgtagac
    12781 aatcaaaaag gtctctttca gcggatggat aagctgggct tagaatatgt ttctctaatt
    12841 ccttttacta ctcatattta tctttcaaaa acgcatccat tggctaatcg cgaagcgctt
    12901 tatttgaacg atattcaagg attacctgcg gtaaggttta cccaagaaag ggacgagtat
    12961 ttgtattatt ctgaaaattt tgtggatact agtgaatgcc ctcgtattta taatgtgtca
    13021 gatagggcaa ccttgaacgg tattttagag agaaccaatg cctttgcgac aggatcaggt
    13081 tttctcgatc atcggagtgt taatgggatt aaagtgatac ctctagctga tcatattgac
    13141 aatcaaatga tttatgtcaa acgaaaagat aaaaaccttt ctgttgctgg ggccactttt
    13201 gtgactattt taaaagacta ttttgataaa agaaggaagg ccttaacatg aaaaaacgat
    13261 tgtttgtgct tagcttgatc ctccttgtag ctttggatca acttagtaaa ttttggattg
    13321 tttctcatat agcgcttgga gaagtgaaac cctttatccc aggtatcgtc agcttgactt
    13381 acttgcaaaa caatggggct gccttttcca tattgcagga ccagcaatgg ttctttgttg
    13441 tcataacggt tttagttatc ggttatgcta tttattacct tgctactcat ccccatttaa
    13501 atatctggaa gcaattagct ctcttgctta ttatttctgg tggaatcggg aattttattg
    13561 atcgtttgcg tttagcttac gtgattgata tggttcattt agactttgtg gattttgcca
    13621 tttttaatgt ggcagattca taccttaccg ttggtgtcat attgttagtg atatgtttat
    13681 ggaaagaaga ggattatgga aattaatgtg ataacatcgg ggcaacgctt ggataaggca
    13741 ctagctgatt taagtcccct gtcacgtggt caagccaatg accaaattaa gcaaggcctt
    13801 gttttagtta atggtcagca aaaaaaggct aaatatacgg ttcaagcagg agatgtgatt
    13861 tgttttgaac tccctaaaga agaagtctta gagtaccaag ctcagaatat tcccttggac
    13921 attatctatg aagatgatgc tctcgccatt attaacaagc cacagggaat ggtggttcac
    13981 ccttcggcag gacacccttc aggaacaatg gttaatgcct tgatgtatca tatcaaggat
    14041 ttgtcctcaa tcaatggtgt ggttcgtcca ggaattgttc atcggattga taaggacacc
    14101 tcaggtctct taatggtagc taaaacagat gcagctcatc aagccttggc agaagaatta
    14161 aaggctaaaa aatctctcag aaaatacctc gccattgtcc atggaaacct accgaatgat
    14221 cgcgggatga ttgaagctcc tataggtcgt agcgaaaagg accgtaaaaa acaggctgtt
    14281 acagcaaaag gcaaagaagc agtgacacgt tttacagtgc tagaacgttt tggcgattac
    14341 agtctagtgg aattacaact ggaaactggt cgaacgcacc aaattcgtgt tcatatggcc
    14401 tatattggtc atcctgttgc tggggaccct ttatatggac cacgtaagac actatcaggt
    14461 catggccaat ttcttcatgc aaaaacattg ggattaacgc atcctatgac aggaaaagaa
    14521 atgatattta ctgttgaagc tcccgaaatt tttcaaaagg tgttaaagct gctaagaaaa
    14581 aaataaacag cgaggagtag ccattatttt tagtcttata tctttgtgca aagtttttaa
    14641 cattataaaa aatactaaaa agcatttacg ataagccagg aaatatgaga agtcttttat
    14701 gttattggga tatgcaacat atctgttaga gatctttatt taaactcctt aaagaaaaga
    14761 gatattccaa atagatgcta ggtattgtct tttttacttg tttttctttt ctttttttgc
    14821 tatactgatt gaaataaaat attcctttaa aacagtcctg tgaggctgtc aaggagacgg
    14881 attaaaaaca tgcaccatga tacttaattg tgctatattt tgctgaatct ccttgtttcc
    14941 aaggagatct ttttatttga atttttagag gaggtttcaa catgaaaact aaggaaattg
    15001 ttgatgacgt gaccatgaaa cgcgccatta cccgtattac ttatgaaatt attgaacgta
    15061 ataaacaatt agacaatgtg gttttggcag gcatcaaaac gagaggtgtt tttctagctc
    15121 gtcgcattca agaaagactg catcaattag aagggttgga tttaccaatt ggtgagttag
    15181 acatcaagcc ttttagagat gacatgagag ttgaggaaga cacaactctc atgtcagtag
    15241 atattacagg caaggatgtg attttgattg atgatgtgct ttatactggt cgtaccatcc
    15301 gcgcagccat cgataactta gtgagtttag gtcgaccagc ccgtgttagt ctagctgttt
    15361 tggtggatcg tggccatcgg gaattaccga ttcgcgcgga ttacgttggg aaaaatattc
    15421 caacaagcag tgtagaagaa attgttgttg aggttgtcga agtcgatggc cgtgatcgtg
    15481 ttagcattat tgacccaaca taagaaagag gttttactgt gaaagatgtc atttatgatg
    15541 tggaagaagt gccaaaggca ggtatgttag ttggtttgtc ctttcagcac ttatttgcta
    15601 tgtttggtgc gactgttctg gttcccattc tagttggaat tgacccatca gttgctttgt
    15661 tatctagtgg tttaggaacc cttgcccact tatcagtgac caaatttaaa attccagcct
    15721 acatgggctc aagttttgct tatatagcag ctatgcagtt actgatgaag acaaatggta
    15781 ttggtgctgt agctcaaggg gctatgacag gaggtctcgt ttacctgatt gtagctttga
    15841 ttgttaaagc tattggcaat gattggattg ataacatctt accaccgatt gtggttggtc
    15901 cgattgttat ggtcattggt ctaagcctag cttctacagc tgttaatgat gtcatgctaa
    15961 aaaatgggaa ctataatctg acttatcttg ttattgggtt agtcacctta ttatcagtta
    16021 tttttttcaa tatttatggt aagggaattg ttgctattgt accgctttta ttagggctat
    16081 tagttggtta tgttgtagcc cttttagtgg gtgttctcac cggtcaagaa attgttgatt
    16141 ttaccaatgt ggctcaggcc aaatggttta gtatcccatc agtggaaatt cctttcttaa
    16201 cctacggggt taaattctac ccaagtgcca ttttaactat ggcacccatt gcctttgtta
    16261 caatgacaga acattttgga cacattatgg tcttaaatag tttgacaaaa agagattatt
    16321 tcaaggatcc aggacttgaa aagactttaa ctggtgatgg ttttgcgcaa atcattgccg
    16381 gcttcttagg ggcacctccg gtcacttctt atggtgaaaa tatcggtgta atggctttga
    16441 ataaaatatt ctcggtttat gttattgcag gtgccgcagt gattgccgct ctcctcagtt
    16501 ttatcggtaa ggtatcagcc ttaattcaat ctattccaac accagttatt ggaggtatat
    16561 cagttgcctt gtttggtgta atagcttcta gcggtttgaa aatcttgatt gaatccaaag
    16621 ttgatatgga taataagaaa aacttattga ttgccagtgt tattttggtc tctgggattg
    16681 gaggattgat gcttcaagta aatggtcttc aaatttcagg tgtcgctttt tcaacacttc
    16741 ttggcatcat cttatatcag gtacttcctg aaaaatagga agaattgttg ttagaccact
    16801 tacatagtca tcagtcgtta gtgataaagg ggataatcat gtcagttgta aataatcgcg
    16861 ttgcacttac caatttagtt tcaatggaag cattaaccac cgaagaagtt ttaggtctta
    16921 tcaaccgtgg ttcagaatat aaggctggta aggttgttat ttcggatcat caaaaggatc
    16981 ttgttgccaa tttattcttt gaaaactcca cccgaaccca taagtctttt gaagtggctg
    17041 agaagaaact cggcttgaca gttcttgatt tcaatgctga tgcaagtgcg gttaacaagg
    17101 gagaaagttt gtatgatact gtgttaacca tgagtgcact tggaacagac atctgtgtga
    17161 ttcgtcaccc agaagatgat tattacaagg aattagtaga aagtccaact attacagctt
    17221 ccattgtcaa tggaggagat ggttctggac aacacccgag tcaatgttta cttgacttat
    17281 taaccatcta tgaagaattt ggacactttg aaggcttaaa gattgcgatc gctggtgact
    17341 taactcactc tagggtggca aagtccaaca tgcaaatcct caaaagatta ggagcagagc
    17401 tttatttcta tggacctgaa gaatggtatt cagaagcctt taatgcttac ggcacttata
    17461 ttgctattga ccagattatt aaggaattgg atgttcttat gttgctgcgt gtacagcacg
    17521 aacgtcatga cggccatcag agtttttcaa aagaaggtta ccatcaagcg tttggattga
    17581 cacaggaacg ttaccaacaa ttaaaagatt ctgctattat tatgcatcca gctccagtta
    17641 atcgggatgt ggaaattgca gatagtttgg tggaagctcc taaagcgaga attgtatctc
    17701 agatggctaa tggtgtcttt gttcgcatgg ctattattga agccattttg aatggtagaa
    17761 ataaaaactc atgatgaaag attaaagcat gtttagaagg taggaaaagg taggaaaatg
    17821 acaaaacgat tacttatttt agaagacggc actatttttg aaggagaacc ctttggtgct
    17881 gatattgatg tgactggtga aattgtcttt aatacaggaa tgacaggcta ccaagaatcc
    17941 atcactgacc aatcctacaa tgggcaaatt ttaaccttta cctatccttt gattggaaat
    18001 tatgggatta atcgagatga ctatgagtcc atttcaccca cctgtaaagg ggttgttgtg
    18061 tctgaagtca gtcggttggc aagtaattgg cgcaagcaaa tgacgctaga tgcgttcttg
    18121 aaaataaaag ggattccagg gatttcggga attgatacga gagctttgac caaaattatt
    18181 cggcaacacg gtaccatgaa agctacgatg gcagatgatg gagatagtat ccagcattta
    18241 aaagatcaac tgcgtgcaac agtcttaccg actaacacca ttgaacaagt gtcaactaaa
    18301 acagcttatc ctgcaccagg gatcgggaaa aatattgtat tggttgattt tggtttaaaa
    18361 cattctattt taagagaatt ttctaaaaga caatgtaata tcaccgttgt ccctttcaac
    18421 atcactgcag aagaagtgct ccaactaaat ccggatggct tgatgctatc taatggccca
    18481 ggaaacccag aagatttgcc agaggcttta gacatgattc gtggggtaca aggtaagatt
    18541 cctatttttg gtatttgtat gggacatcaa ctctttagtc tagcaaatgg tgccaagact
    18601 tgtaaaatga cctttggtca tcgtggcttt aaccatgccg ttagagaaat tgcaacggga
    18661 agaattgatt ttaccagtca aaaccatggt tatgccgttg aacgtagtag tctacctgat
    18721 actttaatgg ttacgcatga agacattaac gataagacgg tcgaaggagt gaaacataga
    18781 gattttccag ctttttcagt ccaatttcac cctgatgcgg ctccaggacc tcatgatgcc
    18841 agctacttat ttgacgaatt tttggaaatg attgattctt ggagatgtac gagtaaatag
    18901 taaatcagtt gcaatttcac gactatatca cctcttgtta ctctatttat taacatttac
    18961 agttgattta gtaccctatc aaaaatatta tttaatgttg ctctgtgagg caacaaatag
    19021 agaaaggaag gcaaggagaa cacaagtaaa taatttagta aaaagttagg ttttcaaaga
    19081 gataaagctc ttattgagaa gtgctgactt taccgcttct tgtggtattc ttggtatcat
    19141 taattatgcc aaaacgaaaa gatattcaaa aaatcatggt tatcgggtct ggcccgatta
    19201 ttattggaca agctgctgaa tttgattatg cagggactca ggcctgtttg gctctaaaag
    19261 aagaaggcta taaggtcata cttgttaatt caaatcctgc aacgattatg acagataaag
    19321 aaatcgctga taaggtatat attgagcctt tgactcttga atttgttaat cgtatcattc
    19381 gaaaagaaag gccagatgcc atactgccaa ccttaggtgg tcaaacgggc ctcaatatgg
    19441 caatggcatt atctaaagct ggtattcttg atgatttaga gattgaattg ttaggtacta
    19501 agttatctgc gattgatcag gcagaagaca gagacctttt caaacaatta atgcaagaac
    19561 tagaccaacc cattccagaa tctacaattg ttaaaacagt tgatgaagct gttacatttg
    19621 ctagagatat cggttatcca gtcattgtgc gcccagcttt tactcttggt ggaactggtg
    19681 gaggaatatg tagtagtgaa gaagaacttt gtgaaattac tgaaaacggc ctaaagttat
    19741 caccggtcac acagtgtttg attgaacgct caattgctgg cttcaaagaa attgaatacg
    19801 aagtaatgcg agatagtgct gataacgcgc tggttgtttg taatatggaa aactttgatc
    19861 ccgtagggat tcacacagga gattctattg tatttgcacc aacacagacc ctttctgata
    19921 ttgaaaatca aatgttacgt gatgctagtt taaaaataat tcgagctctg aaaattgaag
    19981 gtggctgtaa cgtccagtta gcactagatc cttatagttt caaatattat gttattgaag
    20041 taaatcctag agtgtctcgt tcctctgccc ttgcttcaaa agcaacaggt tatcccattg
    20101 ccaaattggc tgcaaagatt gctgttggcc taacacttga tgaaatgatt aaccctatca
    20161 caggcactac ttacgctatg tttgaacctg cccttgatta cgttgttgct aaaattccgc
    20221 gttttccgtt tgataaattt gaacatggtg aacgccaact gggaactcaa atgaaagcga
    20281 caggtgaagt catggctatt ggccgtaatt tggaagagtc cttactaaaa gcctgtcgtt
    20341 ctttagaaat tggtgtttgc cataatgaga tgacaagttt aagtaacatt agcgatgaag
    20401 agttagtgac taaagtgatt aaagctcaag atgatcgact tttttacttg tcagaagcaa
    20461 tacgacgagg ttatagtatc gaagaacttg agagcttgac caagattgat ttatttttct
    20521 tggataaatt acttcatatt gttgaaatag agcaagaatt acaaatgcat gttgaccatt
    20581 tggaaagcct caaaaaagca aagcgttatg gattttcaga tcaaaaaatt gcagaaattt
    20641 ggcaaaagga tgaaagtgat atcagagcga tgagacattc tcatagcctg tatcctgtct
    20701 ataaaatggt agatacttgt gcagctgaat ttgatgctaa aaccccttat ttttattcaa
    20761 catatgagtt ggaaaatgaa tctgttcagt cgaataaaga atcaatttta gttttaggtt
    20821 caggccctat tcgtattggt caaggggttg aatttgacta cgcaacggtt cattctgtta
    20881 aagccatcca aaaagctggc tatgaagcca ttatcatgaa ttcaaatccg gagactgtat
    20941 ctacagattt ttcggtatca gataaattat attttgaacc gttaaccttt gaagacgtca
    21001 tgaatgtgat agatttagag cagcctaagg gagtaatagt tcagtttgga ggacaaactg
    21061 ctattaactt agcacaagca ctttctgagg cgggtgttac aatattagga acacaagtag
    21121 aggatttgga tagggctgaa gacagagatt tatttgaaaa ggcgttgaaa gagcttggaa
    21181 tcccacaacc acaaggtcaa acggcaacta atgaagaaga agctttagag gcagctaaaa
    21241 aaattggttt tccagtctta gttagacctt cttacgtact tggtggccgg gctatggaaa
    21301 ttgttgaaaa caaagaagat cttagagaat atataagaac agcggttaaa gcaagtcctg
    21361 aacatcctat cttagtggac tcttatattt tcggtaagga gtgtgaagtg gatgccattt
    21421 cagatggaaa atcagtctta attcctggta ttatggagca catcgaaaga gcaggtgttc
    21481 attcaggaga ttcaatggca gtttatccgc cacagcaatt atctaagcaa attcaagaaa
    21541 caatagctga gtatactaaa cgtttagcca ttgggttaaa ttgtattggt atgatgaatg
    21601 ttcaatttgt tattaaaaat gaacaagttt atgttattga agtgaatcca cgcgctagtc
    21661 gtaccgtacc tttcttatca aaagtaacag gtattccgat ggctcagatt gctactaagt
    21721 tgattttagg tcaaacatta aaggatctag gatatgaaga tggtctttat ccccaaagtc
    21781 aactggtgca tatcaaagcg cctgtgtttt catttacaaa gttggcacag gttgatagtc
    21841 ttttaggtcc tgaaatgaaa tcaactggag aagtaatggg ttcggatact agtcttgaaa
    21901 aagcacttta taaagcgttt gaagccaata attctcattt atcagaattt ggtcaaattg
    21961 tctttactat agcagatgat agtaaggcag aagctctaag tctagcaaga cgatttaagg
    22021 cgattggcta tcaaattatg gctactcaag gtacagcagc ttactttgct gagcaaggct
    22081 taagcgcgtg tttagttgga aaaattggtg atgctgccaa tgacatcccg acactcgtgc
    22141 gacatggtca cgttcaggca attgttaata cagttggaat taaacgcaca gctgataaag
    22201 acggacagat gattagaagt tcagctattg aacaaggagt tcctttattt acagctttag
    22261 atactgcaaa agctatgtta acggttttag aaagcagatg ctttaacatc gaagcgatat
    22321 aaaaattaaa aaatcatttt taagaaaact taaaaatgat ttttttagat tataatgaca
    22381 aaagtttcag taagctatta atttgtcata gcatataagt cattatcttg tggcttgttt
    22441 tcgactaagg gctaagaatt tcttgacaac aatataaaaa ggtcatatac ttgtcttata
    22501 caaatagtac aaaaggggag gatagaaatg tctaaaagag gaaaaattaa aataacaacg
    22561 aaaacaaagc ttattacagc tagtgttata acgctagtat taattataac tggagtagtc
    22621 ttgtggaaac aacaacaaaa tacgctaaca gctgatatcg ctaaagaacc ttactctact
    22681 gttagtgtaa ctgaagggag tattgcttct tcgactttac tatcaggtac tgtaaaggct
    22741 ttatcagagg aatatattta ttttgatgct aataaaggaa atgatgcaac tgttacagtt
    22801 aaagtaggtg atcaggtaac gcagggccag caattagttc aatataatac aacaacagct
    22861 cagtcagctt atgatactgc tgttaggagt cttaacaaga ttggccgaca aattaatcat
    22921 cttaaaacat acggagttcc tgctgttagt acagaaacta ataaagatga agctaccggt
    22981 gaagagacga caacaacagt tcaaccatca gctcagcaaa atgctaatta taaacagcag
    23041 ctgcaagatt taaatgatgc ttatgcagac gcacaagcag aagtaaataa agcgcagata
    23101 gcgttaaatg atacagtagt tatcagtagt gtctctggaa ctgttgtgga agtaaataat
    23161 gatattgatc cttcttcaaa gaacagtcaa acacttgttc acgtagcaac cgaaggacag
    23221 cttcaggtga aaggaacatt gacagagtat gatttagcaa acgttaaggt tggtcaatct
    23281 gtaaaaatta agtctaaagt ttattctaat caagaatgga ctggaaaaat atcatatgtt
    23341 tcaaactatc caactgagtc taatgcaggt tcaacaacgc cagcaggtag cactggagcg
    23401 ggaagttcta caggagctgc ctatgattac aagattgata ttataagtcc tctcaaccag
    23461 cttaaacaag gtttcactgt ttctgttgag gttgttaatg aagctaaaca ggccttagtt
    23521 cctttaacgg ctgttattaa gaaagataaa aaacactatg tttggactta tgatgatgct
    23581 actggcaaag ccaaaaaagt agaggtgaca cttggaaacg cagatgcaca acaacaagaa
    23641 attcataaag gagtagctgt tggtgacatt gttattgcca atccagataa aaatatcaaa
    23701 ccggataaaa aactagaagg ggttatttca ataggtacca acacaaaacc ggaaaaagat
    23761 tctcaatcaa agaataaaaa atcaggggtg gataaatagt gtcgcatgaa aaaaaacaat
    23821 taatgcaatt atcaaatatt gtgaagagct atcaaaatgg ggatcaagtt cttaaagttt
    23881 tgaaaggtat taatttaaca gtatatgagg gtgaattttt agctattatg ggtccgtctg
    23941 gctcgggaaa atcaacacta atgaatatta ttggtttatt agatcggcca acatccggag
    24001 attacacatt acataatact aaaatagaaa tccttaatga tcgtgaatta gcaaaagtac
    24061 gaaacgatga aatcggcttt gtctttcaac aattctttct gttagctaaa ttaactgcat
    24121 tacaaaatgt tgagttgcct cttatttatg ctggtgtaaa cgtctcaaaa agacgtgaac
    24181 aggctaagca atttcttgaa aaagtaggac ttggacgacg tatcaaacat ctaccgtcag
    24241 aactgtcagg aggtcaaaag caaagggtgg ctatcgcacg agctttagta aatgacccat
    24301 ctattatttt agcggatgaa ccaacaggtg cactagatac taaaacaggt cagcaaatta
    24361 tggaactctt gacagagtta aacaaagaag gtaaaacaat tattatggtt actcatgaac
    24421 cagaaattgc tgattttgca actcggaaaa ttattattcg tgatggtgat atcacaacgg
    24481 atactacagc tagtgttgtt attgactagg aggaaaaata atggaaaatt ggaaattcgc
    24541 tcttagctca atttgggggc ataagatgcg ctcaatcctg acaatgttgg gaattatcat
    24601 cggtgtagca gcagttgtga ttattatggg gttgggaaat gctatgaaaa atagtgtaac
    24661 aagcacattt tctagcaaac aaaaagatat tcaactctat tttcaagaaa aaggagaaga
    24721 agaagacctg tatgccggat tgcacacgca tgaaaataat cacgaagtga agccagagtg
    24781 gttagaacaa attgttaaag atattgatgg tattgattct tattacttta ccaattctgc
    24841 tacttctact atttcgtatg aaaaaaagaa agtagacaat gcttcgatca taggggtaag
    24901 taaagattat tttaatataa aaaactatga tatcgtagct ggtcgtacac taacagataa
    24961 tgattattct aatttttcgc gtatcattct tttagataca gtattagccg atgacctatt
    25021 tggcgaaggt aattataagt cagctttaaa taaagtggta agtttatctg ataaagatta
    25081 tttagttatt ggtgtttata aaactgatca gacgccagtt tcttttgatg gattatcagg
    25141 cggcgctgtc atggcaaata ctcaggtagc ttcagagttt ggcaccaaag aaataggatc
    25201 tatctacatt catgtgaacg atattcaaaa cagtatgaac ttaggaaatc aagcagctga
    25261 tatgctaacc aacatatcgc atattaaaga tggacagtac gctgttcctg ataatagcaa
    25321 aatcgttgaa gaaatcaata gtcagttcag tatcatgaca acagtcatag gttcaattgc
    25381 agcgatctct cttttagtgg gaggaattgg tgttatgaat attatgttgg tatctgtcac
    25441 agagcgaact agggaaattg gtttacgtaa agcacttggt gcaacacgcc tgaaaatttt
    25501 atcacaattt ttgattgaat ccgttgttct aacagttctt ggaggcttga taggattatt
    25561 attagctcaa ttaagtgtag gggctttagg aaatgcgatg acattaaaag gagcttgtat
    25621 ttctcttgat gtggccttaa tagcagtttt attttctgcc tcaataggtg tcttttttgg
    25681 aatgttgcca gctaataaag ctagtaagtt agatccaatt gaggcattac gttatgaata
    25741 gaatagttcg tatcaattaa tctatagacg ctgactagtt taacgtctga ttatatctag
    25801 tttttgtatt agcacagtag ttatatcatt gaagtgttag tgcatttttt gaggtgataa
    25861 tatctagcaa agctatcatt ggtatgatga tgtggatagc gtataagtga tacatctctt
    25921 atttctagct gaacttcggt tcggctagga agttgtcagg caatgagttg tttaaaataa
    25981 aggagattag ttcaatgaca tttttatctg atttgatatc attaatgaca aaaatcagat
    26041 tatcttgggt aataaaggcg ggtatttttc aattattatt tgtaacgatt gctaatattg
    26101 tcttatcaga atttttctat tttatattag acgttactgg tcaatatcat ttagataaag
    26161 acaatgttgt gactttttta aaaaatccta tagcacttgc tttattaggt gcctatttat
    26221 ttttattagc tgcttttatt caccttgagt tttttgctct atatcgaatt attgcggatc
    26281 aagaaattag tttctatctt tttagaaaac agttttctta ttacctaagg gggctttgga
    26341 aaacattttc tggttaccaa ttattacttt ttttgcttta tatcctactg actattccag
    26401 tcttacatat tggtttatct tctgtgatta ctcaaaagct ttatcttcca gaatttattg
    26461 ttggggaatt atcaaagata actagcacaa agtacttgct ttatggcagt cttattcttg
    26521 tgttttacct taacctaaga ttagtatatt ttttaccatt gatagcaatc aaccatcgta
    26581 cggttgctca agcatggaga gagagttggc aaaagactaa aaagaaacat gtattgttat
    26641 ggatgaaact ttttgcaatc aatggtctta cgcttgtagt cttatcgcta gctatttcca
    26701 tgattcttat ttttgttgat atgtttaatc ctaaggggaa taatattatt gttcagctgg
    26761 gagctttgac ctttacatgg gagctcattt tttttactac tatttttttt aaactctgtt
    26821 cagcaatgat tttaaaagag gcaattgaac cacaaaagca atatgatgag ccaagaagaa
    26881 gtaataaggc atatgttgta atctttatcg tggtgacagt aggttttgct tatcaatctc
    26941 ttgaacgttt aacttttttt gacacatctc actctaagac agttatcgcg catagaggac
    27001 ttgtatcagc aggtgtagaa aattctctgg aagcccttga aggtgctaag aaagcaggaa
    27061 gtgattatgt agaactggat ctaatcttga ctaaggataa tcactttgtg gtgtctcatg
    27121 ataatcgatt gaagcgttta gctggagtaa ataagacgat tcgcaactta accttaaaag
    27181 aagttgaaca tctaacgagt catcaaggac atttttcagg gcgttttgtt tcttttgaca
    27241 ctttttatca aaaggctaag aagttgaata tgccattact tattgaactc aagccaattg
    27301 gtacagaacc tggaaattat gtcgatttgt ttttagaaac ttatcatcga cttggtataa
    27361 gcaaagataa taaagtcatg tctttagatt tagaagtaat agaagctatc aagaaaaaaa
    27421 atccatcaat tacgactggt tatatcatac caattcaatt tggctttttt ggagatgaat
    27481 ttgttgattt ctatgtcatt gaagactttt cttatcggtc ttatttgtcg tcccaagctt
    27541 tttggaataa taaagaaatt tacgtttgga ctattaatga tcccaagcgc atagagcatt
    27601 atctcctaaa gcctattcag ggaattatta cagatcaacc agctttaact aatcaattga
    27661 ttaaagactt aaaacaagat aattcttatt ttagtcgatt agtcagaatt attagtagtc
    27721 tttattaaag gtttaaagaa agtgtcaaga aaaaaacttg acactttctt taaactgagt
    27781 tatactgttt aacataggaa tttagcgtag ctgtatttct tatacattac taaaagaaaa
    27841 gagactattt aaaaatggca gtaaaaatcc gtttaactcg tatgggttct aagaaaaaac
    27901 ctttctaccg tattaatgtt gcagattcac gtgcaccacg tgatggtcgt tttatcgaaa
    27961 cagttggaac atacaaccca ttagtggctg aaaatcaaat tactattaaa gaggatcgtg
    28021 ttcttgagtg gttatctaaa ggagcgcagc cttctgatac cgttcgtaac atcttgtcta
    28081 aagctggtgt tatggctaag ttccacgacc aaaaattctc taaataataa cagcctatgg
    28141 ataccattga aaatcttatt atcgctattg tgaaaccttt gatttcacaa ccagataatc
    28201 ttaccattaa aattgaagat actcctgact ttttagagta tcatcttgac ttggatgctc
    28261 aagacatcgg tcgtgttatc ggcaaaaaag gtcgaactat tactgcgata agatcgattg
    28321 tctactcggt tccaacacta ggaaaaaaag ttcgcttagt tattgatgag aaataatcac
    28381 taacttgaga tggattgaaa agcttgttga ctttaagtca acaagctttt tgaatagcaa
    28441 aaatcttaat atttctgaaa tgaaaagatt agatttttct gtctttaact aaaaagaagt
    28501 taaagaatgg cttagctgat tgcctttata agagattata ctggtttttg atagctgctg
    28561 tcatgtaaaa tagctacaat tcatcttacg agattgacct tagcattgcc agtgcagtct
    28621 ttattttcca acctgctgtt tgttgtatgt ttatagtttc ctaaaaaatg ttctctcagt
    28681 tttttggggg acattcctaa aattaagtat acttaatttt aggaatgttt atattcttta
    28741 cattctgatg aaaatacaaa ttatcctaac atgatgtatt gaaaggagaa atagtttatg
    28801 ttagtatact gattaagtga agctgtaatg aggcttatac gatcaaaaaa gttagagtaa
    28861 ctttataaaa cagtggtgtt gaagcaagtt tagcttcttc tcagaaatag attatctata
    28921 aaaaatccca aaagtagttt gtttccagtt aatgaacttc ttttgggaag gcttttttgc
    28981 tgatcgccac ttcacttgta tatcgggaac attcacgcta agatgtataa gttgaatcgt
    29041 ttgtctatgg cttgatgaaa attaatcaaa agataatgtt caactctata attagtacaa
    29101 gcaaaatgag ataacttatt cattagtaag cataagatat aacaagagaa attaccttaa
    29161 aacaggaagt actagagtat ttaaaataag tgatagactt ggatattatt aggtcgacta
    29221 gtgttctttt caataattta atatcgttat tattagtatc agccaatcaa atgtgaataa
    29281 gttggctttt agaaaggttt taccacaatg aagaaacatc ttaaaacagt tgccttgacc
    29341 ctcactacag tatcggtagt cacccacaat caggaagttt ttagtttagt caaagagccg
    29401 attcttaaac aaactcaagc ttcttcatcg atttctggcg ctgactacgc agaaagtagc
    29461 ggtaaaagca agttaaagat taatgaaact tctggccctg ttgatgatac agtcactgac
    29521 ttattttcgg ataaacgtac tactcctgaa aaaataaaag ataatcttgc taaaggtccg
    29581 agagaacaag agttaaaggc agtaacagag aatacagaat cagaaaagca gatcacttct
    29641 ggatctcaac tagaacaatc aaaagagtct ctttctttaa ataaaacagt gccatcaacg
    29701 tctaattggg agatttgtga ttttattact aaggggaata cccttgttgg tctttcaaaa
    29761 tcaggtgttg aaaagttatc tcaaactgat catctcgtat tgcctagtca agcagcagat
    29821 ggaactcaat tgatacaagt agctagtttt gcttttactc cagataaaaa gacggcaatt
    29881 gcagaatata ccagtagggc tggagaaaat ggggaaataa gccaactaga tgtggatgga
    29941 aaagaaatta ttaacgaagg tgaggttttt aattcttatc tactaaagaa ggtaacaatc
    30001 ccaactggtt ataaacatat tggtcaagat gcttttgtgg acaataagaa tattgctgag
    30061 gttaatcttc ctgaaagcct cgagactatt tctgactatg cttttgctca cctagctttg
    30121 aaacagatcg atttgccaga taatttaaaa gcgattggag aattagcttt ttttgataat
    30181 caaattacag gtaaactttc tttgccacgt cagttaatgc gattagcaga acgtgctttt
    30241 aaatcaaacc atatcaaaac aattgagttt agaggaaata gtctaaaagt gataggggaa
    30301 gctagttttc aagataatga tctgagtcaa ctaatgctac ctgacggtct tgaaaaaata
    30361 gaatcagaag cttttacagg aaatccagga gatgatcact acaataaccg tgttgttttg
    30421 tggacaaaat ctggaaaaaa tccttatggt cttgctactg aaaataccta tgttaatcct
    30481 gataagtcac tatggcagga aagtcctgag attgattata ctaaatggtt agaggaagat
    30541 tttacctatc aaaaaaatag tgttacaggt ttttcaaata aaggcttaca aaaagtaaaa
    30601 cgtaataaaa acttagaaat tccaaaacag cacaatggtg ttactattac tgaaattggt
    30661 gataatgctt ttcgcaatgt tgattttcaa aataaaactt tacgtaaata tgatttggaa
    30721 gaagtaaagc ttccctcaac tattcggaaa ataggtgctt ttgcttttca atctaataac
    30781 ttgaaatctt ttgaagcaag tgacgattta gaagagatta aagagggagc ctttatgaat
    30841 aatcgtattg aaaccttgga attaaaagat aaattagtta ctattggtga tgcggctttc
    30901 catattaatc atatttatgc cattgttctt ccagaatctg tacaagaaat agggcgttca
    30961 gcatttcggc aaaatggtgc aaataatctt atttttatgg gaagtaaggt taagacctta
    31021 ggtgagatgg catttttatc aaatagactt gaacatctgg atctttctga gcaaaaacag
    31081 ttaacagaga ttcctgttca agccttttca gataatgcct tgaaagaagt attattacca
    31141 gcatcactga aaacgattcg agaagaagcc ttcaaaaaga atcatttaaa acaactggaa
    31201 gtggcatctg ccttgtccca tattgctttt aatgctttag atgataatga tggtgatgaa
    31261 caatttgaca ataaagtggt tgttaaaacg catcataatt cctacgcact agcagatggt
    31321 gagcatttta tcgttgatcc agataagtta tcttctacaa tggtagacct cgaaaagatt
    31381 ttaaaactaa tcgaaggttt agattattct acattacgtc agactactca aactcagttt
    31441 agagacatga ctactgcagg taaagcgttg ttgtcaaaat ctaacctccg acaaggagaa
    31501 aaacaaaaat tccttcaaga agcacaattt ttccttggcc gcgttgattt ggataaagcc
    31561 atagctaaag ctgagaaggc tttagtgacc aagaaggcaa caaagaatgg tcagttgctt
    31621 gaaagaagta ttaacaaagc ggtattagct tataataata gcgctattaa aaaagctaat
    31681 gttaagcgct tggaaaaaga gttagacttg ctaacaggat tagttgaggg aaaaggacca
    31741 ttagcgcaag ctacaatggt acaaggagtt tatttattaa atacgccttt gccattgcca
    31801 gagtattata tcggattgaa cgtttatttt gacaagtctg gaaaattgat ttatgcactt
    31861 gatatgagtg atactattgg caagggacaa aaagacgctt atggtaatcc tatattaaat
    31921 gttgacgagg ataatgaagg ttatcatgcc ttggcagttg ccactttagc tgattatgag
    31981 gggctcgaca tcaaaacaat tttaaatagt aagcttagtc aattaacatc tattcgtcag
    32041 gtaccgactg cagcctatca tagagccggt attttccaag ctatccaaaa tgcagcggca
    32101 gaagcagaac agttattgcc taaaccaggt acgcactctg agaaatcaag ctcaagtgaa
    32161 tctgctaact ctaaagatag agcattgcaa tcaaacccaa aaacgaatag aggacgacac
    32221 tctgcaatat tgcctaggac agggtcaaaa ggcagctttg tctatggaat cttaggttac
    32281 actagcgttg ctttactgtc actaataact gctataaaaa agaaaaaata ttaaattgat
    32341 ttaaggagaa aaaatgataa cacaagaaat gaaagatctt attaataacc aattagcaat
    32401 ggttgcgacg gttgatgcca aaggtcaacc caatattggt ccaaaacgtt ctatgcgtct
    32461 ctgggatgat aaaacattta tttataatga aaatacagat ggtcaaactc gtattaacat
    32521 tgaagataat gggaaaattg aaattgcctt tgttgatcgt gagcgccttt taggttacag
    32581 gtttgtagga actgctgaaa ttcaaacaga aggtgcttat tacgaagcag cgaaaaaatg
    32641 ggcacaagga cgcatggggg taccaaaagc agttggtatt attcatgtcg agcgtatttt
    32701 taatttacaa tctggtgcta atgcaggtaa agaaataaac agtgaaagta actaaaatta
    32761 actgtaacag tatttaggac ccttatttta aacgctaaaa aacaagcata ctctttttta
    32821 cagcatagtg actatgttgt aaaaaggggt atgcttatta ggtgctctta atatttctag
    32881 ataactgcac ctaataagtt ttcaatagac ttatagcaca ttagttgact tcaattagtt
    32941 gacttcaatt agttgacttc aataccagaa agagaagaac agtgatgttg gtgttcgttt
    33001 aaagaagtat cgatttccac agtgactgag tttatattgt acctttgaca aatcagtcta
    33061 atagattttt tgcaatgttt ttcagtggta gattctttca agcagcaatg tatagttgct
    33121 ctatggtcaa taccatccat cgaccaaaca ttaagctgat taacagacac tatatgagga
    33181 agttggctga gttcatggtg caaagcgcaa tagtctatcg aatcaggaac accatctaaa
    33241 aaaatgttag cggttgctac taactttgga agagctttag ataatataaa agaagcaatg
    33301 gcaattgata ataaaggatc aagaatgtac caaggtttcc attttaagat cagtgacaat
    33361 atgataatgg ctaaccaccc aaggatatct tctaaaaagt gaaggcttaa tatttcttca
    33421 ttttttgtct ggttagagtg aatgatgaag ctagcaaatc cattgattat aattgcaaaa
    33481 atagctaaaa cgaacatacc gtcataatta acaatagtag gatgccataa ttttggtatc
    33541 gtttcaatca ttactaataa tgacccgcta ataagtatta gatttgtcgt tagtgctcct
    33601 aaaaggctaa atcgcttata gcctagtgaa aaatttgaac tctctttttt aacagccttt
    33661 ctttctaaga tagctgagat accaatagca atggcatctc caaaatcgtg gacagcatct
    33721 gctaaaatag cacccgaaaa gaataatgtc ccaaaaataa attcaatgag ggaaaaacta
    33781 agatttaata taaaaataat ggtgactttt ttacttactg gcataatttt ctccttttgt
    33841 tatactggta atatgtatag tagaattctt tattctatta tctaaaagtt aaaatacaat
    33901 tactataacc aaaaatgagc acctgttcaa tagtgaacaa catttagaaa gtgtgtgata
    33961 tgacaaaaga cagacaaatc aaaaaaacaa aaacagctat ctatagtgct tttattgctt
    34021 tattacaaaa aaaagaatat tcgaaaataa ctgttcgaga tatgattaca cttgctaatg
    34081 tagggagatc aaccttttat gcacattatg aaagcaaaga aatgttacta aaagaactct
    34141 gtgaagaact ttttcatcat ttgtttaggc aaaaaagaaa tgtgactttt gaggactatt
    34201 tggtacatat tttaaagcat tttgaacaaa ataaagacag cattgcgacg ttgttacttt
    34261 ctaatgatcc ctatttttta ttaagattta aaaacgaact agaacatgat gtttacccta
    34321 acttgcgctg caagtatatc gacaaaacga ctattccaga agtattccta aaacaatttg
    34381 ttctatctag ttttatagaa accttaaaat ggtggctcca tcaaaggcaa aggatgtctg
    34441 caaatgaact attaaagtat tatttagagt tcataaaata atgactagtg agtcatcttg
    34501 ccaaattcaa ttttttaata cagacggatt ttaacaattt agcagagaaa gacaaaactg
    34561 taagtgcaaa atagactata acgtgataaa ataataggta atttggttct gaaaaagaag
    34621 tgtctatcaa actttagaat caagagaggt cgctaaagtg acgtgagata aagagttaac
    34681 gatttaggag ctaccatgga atattttaat gttggaaaaa ttgtcaacac gcaaggcctg
    34741 caaggagaga tgcgcgtgtt gtctgtcagt gattttgctg aggaacgctt taaaaaaggc
    34801 tcacaattag ctttatttga tgataaagat cggtttgttc aagaagtgac aattgttagt
    34861 caccgtaaac aaaagcattt tgatatcatc aaatttaaag acatgtatca tattaatgct
    34921 attgaaaagt acaagggata cactttaaag gtctcaaaag acaatcaagg ggatttacaa
    34981 gaaggagaat tttattacca tcagattatt ggcatggctg tttatgaaaa ggatgtcctt
    35041 attggacatg ttaaggaaat cttgcagcca ggtgctaatg atgtttgggt agtgaaacgc
    35101 caaggaaaac gagacttgct cttaccatat attcccccag tagttcttag cgtagatgtt
    35161 cctaacaagc gcgtagatgt tgaattgatg gaaggcttag acgatgaaga ttgatatttt
    35221 aacccttttt cctgaaatgt ttgcccccct agaacattcc attgtaggca aagcaaaaga
    35281 aaaaggatta cttgacattc actaccataa ctttcgagat tatgctgaaa aagctcgtca
    35341 tgtggatgac gaaccctatg gtgggggcca aggcatgtta ttgagagcgc agcccatttt
    35401 tgacaccatt gaacagattg aagccaaaaa accaaggatt attcttttag atccagcagg
    35461 taaacccttt actcaggctt atgctgaaga attagctctt gaggaagagt taatttttat
    35521 ttgtggtcat tatgaaggtt atgatgagcg gattaagacc ttggtgacgg atgaaatttc
    35581 attaggtgac tttgtattaa caggaggtga gttggcggcc atgactatag tggatgcgac
    35641 cgtcaggtta attccacagg tacttgggaa agaaagtagc catcaggacg attctttttc
    35701 atcaggactt ttagaatacc ctcaatacac aagaccttat gattatcgtg gtatgacagt
    35761 tccagatgtt ctaatgagtg gtcatcacga acgtattcgc ttgtggcgtc tagaagaaag
    35821 tctcagaaaa acctatttga gaaggccaga tttgttagag cggtacgatt tttccgagga
    35881 agagcgcaag ctattggata agattaaaga agcattgggt taaggagaag attagtgaaa
    35941 gataaagcat atgatattac gattattggt ggtggtccaa taggtttatt tgcagccttt
    36001 tatgcaggtc ttaggggcgt aacagtaaag attattgaga gtttgtcaga acttggtgga
    36061 cagcctgcca ttctttatcc tgagaaaatg atttatgata ttccagctta tccatcttta
    36121 acaggtgttg agttgacaga aaatttaata aaacaattaa gccgttttga agatcgtaca
    36181 accatttgtt taaaagaaga agtattaacc tttgacaagg ttaaaggtgg tttttcgatt
    36241 aggactaata aggcagagca tttttcaaaa gccattatca ttgcttgtgg aaatggcgct
    36301 tttgcaccaa gaactttagg gttagaaagt gagggaaact ttgctgatca taaccttttt
    36361 tataatgttc accagttgga ccagtttgcg ggacaaaaag ttgtgatttg tggtggtgga
    36421 gattcagcag ttgattgggc tttggcttta gaagatattg ctgagagtgt gacggttgtg
    36481 caccgtcgtg atgctttcag agcacatgaa cacagtgtgg aattattgaa aacatcaacg
    36541 gtaaacctct taacacctta tgtgccaaaa gcattgaaag gaattggaaa cctggctgaa
    36601 aaattagtta ttcaaaaagt gaaagaagat gaagtgttag aacttgaatt ggacagtctt
    36661 attgttagtt ttggtttttc aacttctaat aagaatttga aaaattggaa tctcgactat
    36721 aagcgttcaa gtattactgt ttcaccactt tttcaaacaa gtcaagaagg tatttttgcc
    36781 attggtgatg cagccgctta taatgggaaa gtggatttga ttgctactgg ttttggcgaa
    36841 gcaccaacag cagttaatca ggccattaac tatatttatc cagatcgaga taaccgtgtg
    36901 gtgcattcaa cctctttaat tgactaaggg agtcggaagt ttcaagagca gtagcaacag
    36961 ttaatattca gtttgtcagg agaggacaaa tggtttttac ctaagtcttc gtgacaagtt
    37021 ttatactttg tgataaaata gtcattgtga aaaaagtgtg atggttttca cagattaaca
    37081 cattacaatg aaaaggggtc tatcatgaca acaacagata aagagacatt tagttctttt
    37141 atgaataagg tcttggctgg tacagcgatt gctatcgttg tggcactcat tccaaatgct
    37201 attttagcga ctttcttaaa accgcttttg ccaaacatgg cagctgctga atttttacac
    37261 attgtgcaag tcttccaatt ctttacgccg attatggctg gtttcttgat tgggcaacaa
    37321 tttaagttta atcctatgca acagttggct gttggtggag ctgcttatat cggttctggg
    37381 gcttgggctt atacagaagt cattcaaaaa ggtgtggcga caggaacatt ccaacttaga
    37441 ggtattgggg acttaatcaa tatgatgatt acagctagcc ttgcggtttt agctgtgaaa
    37501 tattttggga atagatttgg ctctttaacc attatcttgt tacctatcac tataggaact
    37561 ggcgtaggtt atattggttg gaaatttttg ccttatgtgt cttacgtgac aaccctgatc
    37621 ggacaaggga ttaattcttt tacaacctta caacctattt taatgtctat tttaattgca
    37681 gttgctttct cgcttatcat tgttagtccg atttcaactg ttgcaatcgg tttagctatt
    37741 gggcttaatg gaatggctgc gggagcagct tctatgggaa ttgcatcgac agcagcagtc
    37801 ttagtgtggg cgacgcttaa agttaataaa tctggtgtac caattgctat cgcacttggg
    37861 gctatgaaaa tgatgatgcc aaacttcttg aaacatccta tcatggctat tccgatggtg
    37921 ttcactgcag caattagctc attaacagta ccattgttta atcttgttgg aacaccagct
    37981 tcatctggtt ttggcttagt tggtgcagta ggtccgattg cttctttagc aggtggtaac
    38041 tcaatactta ttattatcct tgcttggatc attgttccgt ttgcggttgc ttttgcggcg
    38101 cataaggttt ctaaagatat tctaaaactt tataaagaag acattttcgt ctttgaaggc
    38161 taataggagg aaaatatgtt agtttatatt gctggctcag gagccatggg gtgccgtttt
    38221 ggttaccaaa tttcaaaaac aaataacgat gtgattttgt tagataactg ggaagatcat
    38281 atcaatgcca ttaaagaaaa cgggttgatt gtaacaggtg atgtggaaga aacagttaag
    38341 cttcctatta tgaaaccgac tgaagccact caagaagccg atttgattat cttgtttaca
    38401 aaagcaatgc agttgccaca aatgcttcaa gacattaaag gcattattgg aaaagaaacc
    38461 aaagtgcttt gccttttaaa tggtcttgga cacgaagacg ttattcgtca gtacattcca
    38521 gagcacaaca ttttgatggg ggtcactgtc tggactgctg gtctagaagg accaggtcgt
    38581 gctcatcttc aaggagttgg tgctcttaac ttacaaagta tggatccaaa caatcaagat
    38641 gcgggacatc aagtagctga cttgctaaat aaagctaact taaatgcgac ttatgatgag
    38701 aatgttgtgc caaacatctg gcgaaaagct tgcgtgaatg gtaccatgaa ttcgacttgt
    38761 gctcttttag attgtacgat tggcgaatta tttgccagtg aagatggtct caaaatggta
    38821 aaagaaatca tccatgagtt tgttatcgtt ggtcaagcag aaggtgtcga actaaacgaa
    38881 gaagaaatca cacaatatgt aatggataca tctgttaaag ctgctcacca ttacccatca
    38941 atgcaccaag accttgtgca aaatcaccga ttgactgaaa ttgattttat caatggtgca
    39001 gtcaatacca aaggtgaaaa gcttggtatc aatactccat actgccgtat gattacagaa
    39061 ctagttcacg ctaaagaagc agtgttgaac atccaataaa cgctatcaaa tcgttatact
    39121 caatattatg gctaaagctt taatattgag ttttttgtta tgctgatagt atttttctta
    39181 ggcgtcatta tttgtgagcc tagattatga gttaataaaa gaaataataa ttggtagaat
    39241 ttgatagatt ttgaaaggtt atgcttgact tttagttata aaatagtata ataattgtaa
    39301 acgatttcag ttaggggtgg ttctatctta aaatcaaaac gaaaacaatt aatcatggca
    39361 aagatcactg aagaaaacta tgtttcttta gaggatttaa tgcaattact caactcctct
    39421 gagtctacta tccgtagaga tctaggagag ttggagcaag aaggacggct acatcgtgtt
    39481 catggaggtg ctgaactttt tcactcttta caagaggaac tttctaacca agaaaagtct
    39541 gtcaaaaaca gtcatattaa aaaagctatt gcgcaaagag cctctcagct tatctatgac
    39601 aacgatgtga tctttattga tgctgggaca acgactgaat ttttattacc ttttttacaa
    39661 gctaaaaatt taactgtagt tactaattct attcatcatg ctgctagatt agttgaactc
    39721 tctattgaga cgattattgt aggtggttat gtcaaacaaa cgaccgatgc cagtattggt
    39781 aatgtagctt tggaacagat taggcagatg aattttgaca aggcatttct tggaatgaat
    39841 ggtgttgatg attcttacct gacgactcct gatatggaag aagcagttat taaaaaagcg
    39901 gttctgtcaa atgcaaaatt agcttatatt ttagtagatg gcactaaaat tggtcaggta
    39961 tcgtttgtaa aagtagctcc tattaatgat gtgactatta ttacattagg aggatcagca
    40021 agtattctta aacaaataaa ggaaaaggca aaggtgattg aattatgatt tataccgtga
    40081 ccttaaaccc ttctattgac tttattgtca gaattgacca aataaatctt gggtcagtga
    40141 atcgcatggc gagtgatgat aagtttgctg gaggtaaagg cattaatgtg agtcgtatct
    40201 tgcaacgttt agacattgct agcacggcga cagggttctt gggtggtttt acaggacgtt
    40261 ttatcgaaga aagtttgagt gcagaaggcg ttaaaactga ttttgtaaaa ggtgatcaag
    40321 atactcgaat caatgttaaa atcaagtcac aagaagagac cgaattaaat ggtcaaggac
    40381 ctatcattag tcgggaacaa ctagaggagt tgaaaactaa gctttcccag ctaacagcag
    40441 aggatacagt tgtctttgca ggttcagcgc cagctaatct tggcaatgct gtttataagg
    40501 aattacttcc tttagttagg caaagcggtg ctcaggtggt ttgtgatttt gaaggacaga
    40561 ctttaataga tgcgcttgca tataatcctc tgttggttaa acctaataat catgaactag
    40621 aagctatttt tggtacgatt ctgactagtc ttgatgatgt tgaaacctat gcccgtcgtc
    40681 ttttagagat gggtgctcaa aatgtcatca tttcaatggc tggtgatggt gctttgctgg
    40741 ttactaaaga agcgacttat tttgctaaac cgattaaggg agaagttaaa aattctgtcg
    40801 gtgctggtga ttcgatggtt gctggcttta caggcgaatt tgtgaagagt caaaatccaa
    40861 tagaagcctt aaaatggggc gtggcttgtg gtacagcgac ggctttctct gatgacttgg
    40921 cgacaattgc atttattaag gaaacatatc ataaagttga ggtagaaaaa cgatgaaaat
    40981 tcaagattta ttgagaaaag atattatgat tctcgattta caggctattt ctaaagaagt
    41041 tgccattgac gagatgatta ctaaattagt tgaaaaagat attgtacatg attttgatgt
    41101 ctttaaaaag agtatcatga cacgtgaaga acaaacatca acagggcttg gtgatgggat
    41161 tgccatgcct cattctaaaa atattgtagt agataaacca gcggtcttgt ttgcgaaatc
    41221 aaataaaggt gtggattata aggctttaga tggtcaacca acggatctct tctttatgat
    41281 tgctgcgcct caaggagcaa atgatactca cttggctgcg ctagcagaat tatcacaata
    41341 ccttttgaaa gacggctttg ctgataaact tcgtgctgca gcgacaccag aagcagtcat
    41401 tgctgttttt gatgaagcgt caacagctaa agaagaagtg gttgctccaa caagtggtca
    41461 agactttatc gttgctgtta cagcttgtcc gacaggaatt gctcacactt atatggcaga
    41521 agaggctttg aaaaaacaag ctgcagaaat gggagtagct attaaagttg aaacaaacgg
    41581 tgcttctggt gtggctaatc gtttaaccgc tgaagatatt cagagagcta aaggagttat
    41641 tgttgcagct gataaagcgg ttgaaatgga ccgttttgat ggtaaacaat tcattgctcg
    41701 ccctgttgca gatggtatca agaagagtca agaattgatt tctttgattt tgaataatga
    41761 aggacacact taccacgcta agaacggaaa gtctgaaaca gcagtatcat ctgagaaaac
    41821 aagcttaggc ggtgctttct ataaacactt gatgggtggt gtctctcaaa tgttgccatt
    41881 cgttattggt ggcgggatta tgattgctct agctttcttg ttggataaca tgcttggtgt
    41941 gccaaaagac caactaggca atcttggatc ttatcatgaa gtagctgcta ttttcatgaa
    42001 aattggtggt gcagccttct ccttcatgtt accagtatta gcgggttaca ttgcatactc
    42061 tatcgctgaa aaaccaggtt tggttgctgg ttttgtggca ggtaccattg cctcaaatgg
    42121 tcttgctttt ggtaaggtac catttgcagc aggaggagaa gtgagcttgg gcttaacggg
    42181 cgtgccatca ggtttccttg gggcgcttgt gggtggtttc cttgccggtg gtgttattct
    42241 tgcccttcgt aaattgttgg caggcctacc acgttctcta gaaggggtta aatctatcct
    42301 cctttaccca ttacttggcg tccttgtcac tggtttcttg atgctttttg tcaatattcc
    42361 aatggcagct attaacacag cccttaatga tttcttacaa ggtctttcag gaagctctgc
    42421 tgtccttatg ggacttcttg ttggtggaat gatggctgtt gacatgggtg gtcctgttaa
    42481 taaggcagct tatgtttttg gtacaggaac tttagcagct actgtcgcaa atggcggttc
    42541 agtcgttatg gctgctgtta tggcaggtgg tatggtacct cctctagctg tttttgtagc
    42601 cactctcttg tttaaagata aatttaccaa agaagaacgt gaatcaggtt tgaccaacat
    42661 tgtcatggga ctttctttca tcaccgaagg agcaattccg tttggtgcag ctgacccagc
    42721 acgtgcgatt cctagcttta tcgcaggttc tgccttgaca ggagctcttg ttggtttggc
    42781 tggtattaaa ttaatggcgc cacatggtgg tatcttcgta atcgctttaa caagcaatcc
    42841 aatcttatat cttgtctttg ttgtgattgg cgcccttgta tcaggtatct tatttggcgc
    42901 tcttcgcaaa aaagcctaaa aggtaaagaa cttttttctt actaaaacta gaatctgtca
    42961 aggttctagt tttttgctat actagagtta aaggataaga aacgaggacc tttgaaggtg
    43021 aaaaaaagga gaagaagggc tagatcatca gtaaatcgac ttgtcttagg gttagtatta
    43081 cttaatctga ttgttagtat gtggacacta aaattgggaa accagcgatt agctccgtat
    43141 gcggatcacg aaactctgac atttgtcagg aagattagtc atgctgctca atcagtcgct
    43201 caaaagaaac agttatacag ttcggtgatg atggctcagg ccattttaga atccaataat
    43261 ggcaagtcac aactaagtca aaaaccttat tataattttt tcgggattaa ggggagctat
    43321 aaagaacggt cagtcatttt tccaacttta gaagacgatg gacaagggaa tctttatcaa
    43381 attgatgctg cttttcgttc ctatgggagc ctgacagctt gttttttaga ttacgcaaga
    43441 gttttgaatg atccacttta tgacaaaacc cacaaaaagt tttggtctca ttatcaagat
    43501 gctactgcaa ccttaacagg cacttacgcc actgatacaa cttaccatac caaattaaat
    43561 gaattgattg aatggtatca actcactaat tttgatggtc taatgaaata gttatccaca
    43621 attttaaaaa aaatcagaag aaatatgtcg atttcaagac aaaagttatc aatctcatta
    43681 aattatctgt ggaaaagtta atttaaacgt tcgtgtgcta taatagaaac atagaaaatg
    43741 tataaaaaag gatatgtgaa taatgacaaa aaagaaaggt aagcttgtac ttatcagtct
    43801 ttttgttcta gcagcttgtt taggagctta tagtgcaatg agacaatctc ataaaacttc
    43861 aaacgtgtca gctgagacta tcgccagttc ttcaacccga cattttattg atgaaattgg
    43921 tccaactgct agtactattg gtcaagaacg tgatctttat gcgtctgtta tgatagcaca
    43981 agcgatttta gaatcaagta atggtaaatc aagtctaagt caagcacctt attataattt
    44041 tttcggtatt aagggtgcct ataacggttc ttctgtgacg atgtcaactt gggaagatga
    44101 tggtaatggc aatacctata cgattgatca agcctttaga gcttatccaa gtattgcaga
    44161 ctcccttaat gactatgcag acttgctaag ttctagcact tatataggtg ctagaaaatc
    44221 aaacacgcta tcgtatcaag atgctacagc agctctaact ggcttatacg ctacagacac
    44281 cagttataac ttaaaactaa ataatattat tgcaacctat ggtttgacgg cttatgatgt
    44341 tgctaatagc tcagctcaag aaactggtct agcaacttcg ggttatgttt ggaatgagta
    44401 tcgtcgtaat tacactgatg cggagaccct agcagttgat gaggcttggg ctaaacgcat
    44461 gacttattaa tgtaacacta acaacaatct gcttgcactg atcccaaaaa agctggatac
    44521 tctattttaa gtaaaggatt tagttctgta ttggacagga ctaaatcctt ttagttttgc
    44581 tttgatatgt ttatcgttgt aataatcaat gtcatttgta atcgcttgtt ctaagtcttc
    44641 tagtgattta tagttattct cataaccata gaacatttct gactttaaga tgccaaagaa
    44701 agattccatc atgctattgt taggactgtt tcttttacgt gacatagacg gacgaattcc
    44761 tttagagttt aggaaatgat gataatagtt gtgttgatat tgccaacctt gatcactcta
    44821 tcatatagtt ccatcatagc atttatttgg agatgtcttt ttccattatt ggtttaatgt
    44881 gatttaagca aggttattag ctaaacttaa tgccagacaa aaacacccca attattagat
    44941 tttttgtcta acttttgggg cgcaattcaa tctgagcagt tttagatttg gtatcaatgt
    45001 tattataggt caattgatca tatttatcca aagcttttag cttatttagt ccaaagataa
    45061 atatcaaaat ggctgaaatc tttcatgttt atagtcttat tgtctttata tttttttaag
    45121 acatcagatc tggtgctatt gggttttgcg tcatataaat tgattttttc atgtttccta
    45181 tttttactgt gaatttctaa actccccctt atataaggag acttagtcaa atgaatttct
    45241 tttgattcca tcaaaaattt tcttatttta aaatcgaact cttgaatcgt aacaatatcc
    45301 ttatcgattt ttataggatt tattaatgtt ttttgctgtt gcccatctat aaaaaaattc
    45361 cctgcaatag tattagtcct tagaatacca tcatctgttg aaggtattct ttcttattga
    45421 tacgctttaa ttggtctgat tccaactatc ttgccctgtt gaaagtgtaa ataaccctag
    45481 tacttgagca ccaatattat cttcttttat ttctttagca gaaatagcta cttttccagc
    45541 ggaattaaca ccaacaaagt atttcttcat accaatagat gcattactat cagagtctgt
    45601 tacataaata gctttaaggt tcccgttaga atcaaagtca gctccccaca ggtttataac
    45661 atggttgatg cgtacgttag cgtaggtgtg tgataggcct agagccttgc cttcggttaa
    45721 ctctttctta atgagatcac tgatttcttt gagatttttt tctttaaaat catgacgact
    45781 tgtcaatagc ttactttgat cacctcttgt aaatacggcg tcaaaaatac cacctcggcg
    45841 atctttctct tgataggttc tattgacatc agtagtctgt gtcttataaa catttaagta
    45901 ataaccattg ataaacatat ctaaaacaag atcaggcata actccaattc ggcgtgctga
    45961 caaaccgggg aaagctttat ctcggaaata attaaaaagc tcgctatttg tttggtcacc
    46021 tttggtatta ataacttttc ttacatccaa taattcttga tcaccaaaca tgattttttg
    46081 tttatctggg tgttttttta gatatgcttc aattttttct ttgttttgat cgaaccacca
    46141 gtgaagcata ttccctgctg tggcagcccc gcaaagaaga tcgtcttttc cattgaatgt
    46201 tttggtaata tcataccatc cttggttagc aacataagga gcgtgaaaaa catcttcgcc
    46261 ttgagtgaat tttgctggag gagtaactcc tttggtccaa acggaagtaa cgtgataagg
    46321 tgttacttcc gaatatctaa tctcttgatt agcagaaaaa ctatctgcta taacaccacg
    46381 atctaccgat agagcaaata aagtcactgc tgccaatact gcagctgaag ttgaatagca
    46441 tctttttctc ataagaatac ctttttctat ataattttat gataaagaaa ttatataata
    46501 tagacatata ttattgcaag tttttaaaat aatatttatt ttttaaaata atttttaata
    46561 aaattaaaca agcgattgac aatggagata tcacaagaga tacagtagtg gttgtgctta
    46621 aaaatggacg gttttttgat gatatgttgc tgcatgagga agtgagcaat aggagagctg
    46681 tgatagaaga aagaggggaa gaagcgttga tgaaattaga caaataaaaa aagacctcat
    46741 ccagaagagg tcgtgatata actggggacg ataggttcaa ttgataaaaa atcttgttag
    46801 cttacgatct atgttgtttt gaatgccttt aactcaacat cgttaatgga gtttgagata
    46861 aaaaaataaa tccaatagtc tggtactttc ctattttttg tctgttaaaa cgtttttatt
    46921 ttataatgga taaaagaaga agggcggagc gatgattaag ccctatgggt ttaagtatgg
    46981 gttagttgga gaagttttgc ggcggattga acgcctagga tttacgtttg agcgactaga
    47041 gctaagacaa gctagttcga aatacttggc taagcatgat gaggccttgg tgataaacct
    47101 ttttatcctg gacttaaaac ttacctgacc agtggttctg ttttaattga ggtgcagtac
    47161 aagaccacta acgggtgttt ttttgatata atgtcagtat aaggacgact aaatataaat
    47221 aggtgaaaaa atagatatct gttcgagaga tagcactgtt taaaacgtta tgcgaaaata
    47281 gtagtttagg ggctacttta ataattttca aatctatgct ataatggatc atattgatac
    47341 caaggagaat aaaaaatgca attagttcgt gaaaaagaat ttgtcaacca gtaccattat
    47401 gatgctagaa atttagagtg ggaaaaagaa aacggaacac cagagaccaa ttttgaagtg
    47461 acttttcagt tgattgataa agatgagcag caaaaagaaa cagtaattgt atctgtattg
    47521 caatttgtta ttgtcaaaga agaatttgtt attagtgggg tcatctcaca aatggttcgt
    47581 attttagatc gtttagtgga taaaccaagc gaatttacgc aagaggaagt tgaatcctta
    47641 gctgctcctt tgctagacat ggttaagcga ttgacttatg aagtcacaga aattgccttg
    47701 gatcgtccag gtattcattt ggagtttaaa aattaatgaa attagcagta attactgaca
    47761 gtacagcaac actaccaaca gatctgaaac aagataaagc tatttttagc cttgatatac
    47821 cagttatcat tgacgatgag acctattttg aaggtcgaaa tttgagtata gatgattttt
    47881 atcaaaagat ggctgactcc caaaatcttc ccaagacaag tcagccaagt ttatctgaac
    47941 tagataatct tttaggactt ttgtcttcta agggttatac acatgttatt ggcttgttct
    48001 tagctggagg aatctcaggc ttttggcaaa atattcaatt tttagctgaa gaacatcctg
    48061 agattgaaat ggcatttcca gacagtaaga tcacatctgc tcctttaggt agtatggtaa
    48121 aaaatgtgct agattggtca cgtcaaggaa tgacatttca agctatttta aacaagctgc
    48181 aagaacagat tgatgggacg acagccttta ttatggttga tgaccttaat catctggtta
    48241 aaggcggacg cctgtcaaat gggtctgctc ttcttggaaa tcttttgagt attaaaccta
    48301 ttttacgctt tgatgaagag ggaaaaatcg ttgtttatga aaaagtgaga acagaaaaga
    48361 aagctatgaa acgcttggtt gagattttaa atgacttgat agcagacggt cagtataatg
    48421 tgtctattat tcactcaaaa gcacaagaca aagctgatta tttgaaacga ctcttacaag
    48481 acagtggtta tcaatatgac attgaagagg ttcattttgg agcggtgatt gctactcatt
    48541 taggagaagg agccattgca tttggcgtaa caccgaggct ttaattgtcg attagaaaaa
    48601 atagttaatt gcaagaatca ctaaaacaat aacaaaatgt gatagaaccc aagtagccta
    48661 atcacacttt agattgggct agttggatga taggttagaa aacaacgtat gaaattaatg
    48721 acaatgcctt ctgagtttca gaaggcttta cccatattga ctaaaataaa agaagctggt
    48781 tatgaagcct actttgtggg aggaagcgtc agagatgttt tactcgagcg tcccattcat
    48841 gatgtagata ttgcaactag ttcctatcct gaagagacta aagcgatttt taacagaact
    48901 gtcgatgttg gcattgaaca tggtacagtg ctcgtgcttg aaaatggcgg tgagtatgaa
    48961 atcacaacct ttaggacaga agatgtctat gttgactatc gccgtcctag tcaggtttct
    49021 tttgtgcgct ctttagagga agacttaaaa cgaagagact ttaccgtcaa tgccttggcc
    49081 ttggatgaaa atggtcaagt tattgataaa tttagaggac tcattgattt ggagcaaaaa
    49141 cggttacgag cagtgggtaa agcagaggaa cgttttgaag aagatgcact acgcattatg
    49201 agaggctttc gatttgcggc gagtctggat tttgacattg aagctgcaac ttttgaagct
    49261 atgaggagcc attcacctct attggaaaag atttctgtgg aacgttcttt tactgaattt
    49321 gataaattat tgatggcacc gcactggcga aaagggattt ctgccatgat tgcttgtcag
    49381 gcttatgact atttaccagg cttgaagcag caggaagcag gcttgaatca tctgatagtt
    49441 tctttgaaag ataactttac ctttagtgat caccaccaag cttgggctta tgtgatgatt
    49501 agtttagcca ttgaggatcc taaaagcttc ctaaaagcat ggaagacttc aaatgatttt
    49561 cagcgttatg tcacaaagtt gattgctctt tatagaattc gtcaggaaag atcttttgaa
    49621 aaattggata tttaccagta cggcaaggaa atggcaagtc tagtggaagg cctccgaaaa
    49681 gctcaaagct tatctgttga catggaccac attgaagctt tagatcaagc gctagccatt
    49741 cataacaaat acgatatcgt ccttaatggt tctcacctga ttaaggattt tgggatgaaa
    49801 ccaggtcctc agctagggct tatgcttgag aaagttgaat tagcgattgt agagggacgc
    49861 ctagataatg atttcactac aattgaagca tttgttagag aggaattagc aacatgagcc
    49921 attttttggt ggagaaattg acaaaaacgg tgggagataa aacagtcttt caggatattt
    49981 cctttattat tcatgacttt gaccgtattg ggattattgg ggttaatgga acaggaaaaa
    50041 caactctttt agatgtcttg tcgggaaggc ttggttttga cggagaccat tcaccatttt
    50101 ctaaagccaa tgattacaaa atcgcttact taacacaaga tcctgaattt aacgatgcat
    50161 cctctgtttt agatacggta ttgtcatctg atgttaaggc gattcagctg attcgtcagt
    50221 atgaactatt gatggcgaat tatactgaag ataaacaaga gaccttagag tccctcatgt
    50281 ctgaaatgga tcgcttagat gcttggtcta ttgaaagtga tgtcaaaaca gtcctatcaa
    50341 aattaggcat cacggatttg gaacaaaaag tgggagacct atcaggtggt atgagaagac
    50401 gtgtccagtt agctcaagta ttgctaggag ctgctgactt actcttgttg gatgagccga
    50461 ccaaccatct cgatattgac accatcgctt ggttaacaac ctatctcaag acagctaaaa
    50521 aaaccgtcct ttttattacc catgaccgtt atttccttga tcatgtggct acccgtattt
    50581 ttgaattgga taaagcggga ctgacagaat atcaaggcaa ttaccaagac tatgtgcgct
    50641 taaaagcaga acaagacgaa cgagatgcta ctaatttaca caagaagaag caactctata
    50701 aacaagaatt agcttggatg aggacgcagc cacaggcgcg tgcaaccaaa cagcaggcta
    50761 gaatcaatcg tttcactgac ttgaaaaaag aagttcacca agacagttct gcagataaat
    50821 tggagatggc tttcgaaacg agccgaattg ggaagaaggt gattcacttt gaagaccttt
    50881 cttttgctta tggcgaccga cagttgatta aggattttaa cttgattatt caaaataaag
    50941 atcgtattgg aattgttgga gataacggcg tcggaaaatc taccctgtta aatatcatca
    51001 acggtgatct caaaccaact tctgggaagc ttgatattgg agatactatt cgtgtaggtt
    51061 atttctctca gcagctcaaa gatatggatg agaccaaacg ggtcattaat taccttcaag
    51121 aagtggctga tgaggttaaa acaagcgtgg gaacaacgag tatcagtgag cttttagagc
    51181 aatttctttt tccgagatca agtcatggca cgctgattgc taaattatct ggtggggaga
    51241 aaaaacggct ctacttgcta aaactgctca ttgaaaagcc aaatgttctt ctccttgatg
    51301 agccaacaaa tgatttagac attgccacct tgacggtctt agaaaacttt ttagctaact
    51361 ttgcaggacc agtcattacg gttagtcacg atcgttattt cctagacaaa gtagccacta
    51421 agattttagc ctttgaagaa ggcgatattc gtatttttta tgggaattac agtgattacc
    51481 ttgatgaaaa agcttttgaa aaagaaacag ttgaggctga cttagctaaa gcaatagtca
    51541 ctgaagaaat gccacttcct ccgaaagagg agaaaaagcg catgtcctac cttgaaaaac
    51601 aagaatgggc tcagatagaa gataaaatag caactattga ggctaatatt gaagaaattg
    51661 aaaaccaaat gttaactgta gttagtgatt atggccagtt agctcaatta caaaaggaac
    51721 tcgatcagcg caataatgat ttattattgg cgtatgaacg gtttgagtat ttgagtggac
    51781 tagacatata agtatactat ggtaatgtta tgtttataat aaacaagtga taatagaaaa
    51841 gaagtgctct tgcactgagc cccaaaagtt ggctactcta tttgaagtaa aggatttagt
    51901 tctgtattgt agaggactaa gttcttttcg ttttattttg atacgtttat tgttgcaata
    51961 atcgatgata atagtaggta gaacgagtca atttagccgt tcttaaaaga aaatctaatc
    52021 agaactcgtc tttgtccatt ctttataatg gttttttgtc ttcttctttg cagagatgct
    52081 tctttcaaac ggagttccct cagtttttaa gcgctaaatg tctagcttat cttcatagct
    52141 taatgtcata caaaaacacc ccaaacgtta gattttttat ctaacgtttg gggtgtagtt
    52201 tacattatgg cgcttctttt ctattattac gtgaggaaaa atcaagtaaa aaagttaagt
    52261 caactgaaaa acttgacaga aatattcaga aaatttataa tactaactat taacaacata
    52321 tcagctatta aagcatttca aacagcaggt gttttgtttg gacctatcaa ggctgtaaat
    52381 gcaagagaaa gcagtatctg gtttgaaaat ctcacaaagc aataactatc tctcttagac
    52441 atttgaagaa gcagacagta aattcgataa tattgtgatg agaacttagc aacaatctgc
    52501 tgatgcaggt caatttttat ttggtactca tattgcagaa tttcataaag ttgcccaaga
    52561 tatcttgaaa taaggaatca cctgatgttt taaacagatg atactaagtc tatcatatgg
    52621 tgataatagt ccattgaaca tgtagtttat gaattaaaaa gaaggtttaa ggagataaga
    52681 atgattagag aaatcataac agatcatttt ttattacagc aaaaagcgca agtcgccaag
    52741 aaagaagatc tatggattgg ccaagattta caagatacct tagcctttta tagacaggaa
    52801 tgtcttggga tggctgccaa tatgattggt gaacaaaagc gcattgttat tgtgagtatg
    52861 ggatttattg atttggtgat gtttaatcct gtgatggttt ctaaaaaggg aatttaccaa
    52921 actaaagaat cttgtttgtc attatctggc tatcgcaaaa cacagcggta tgataagatt
    52981 actgttgagt atttggacca taattggcga ccaaagcgtt taaacttaac aggcctaact
    53041 gctcaaatat gccagcacga attagatcat ctagaaggta ttttaattta acaaaaaacc
    53101 tagagtagga cactttaggt ttttttggcg aaaatgcctg attatataag gatgatcaat
    53161 tactagtaaa cgctaggatt ttcttttttt aatgaaagcg tttagcccac ctaagcttat
    53221 caaggcaagg attgtcataa taggagacat tttataattg tcccctgtta ttggaagagt
    53281 ggaattagag atagtatttt tgtttgttgt tgtctctttt ttgccaaagc tgttattagt
    53341 gttttcagta gaagtcaaaa ggtctgaaat gacttcacta gacactatat tgccatcacg
    53401 atttctaaaa accttactaa tgatactgtg tttgccagca ctattaattt ttgttgatct
    53461 ttcaaggttt gaagttacat aatttttaac ccctttgata gtagcattaa cagttttacc
    53521 tgatgcttgt aaatttgtga tatatgtgat gaaagcttca gtatctgtgt taatagctcc
    53581 gactaattta gcttttttaa atgctgaaaa gccatcaccg ccaccataaa gaaagtcgtt
    53641 gacaacaacg gtgtaagtag ttgttaagtt aatttcttca ccattgtctt tataaacctt
    53701 aactatcttg aagggggtat cagagttctt aggatcatta tctgtataag tgtatgttaa
    53761 acctgacatt tgaagaaaat aggtctggtt ttcatcgtat tgctgattta ggacatcgta
    53821 aatgtgttga ccagtcattt gaatgacttg aaggatatta ccaaatggtt gtacagcctg
    53881 tgcagctccc caggtgatgg tccggtcatt tttgacaact aggtcacttc gaataccacc
    53941 attattggtc atagcaaagt caacagttgg aaaagttttc ttagcaatag taagctgagc
    54001 cgttgttact aagttaccga caggggattc tttatcaata ttttctgttt tagaaatagt
    54061 tgaagaatta gttgcagttc cgatttttcg ttcagtaact gttttaacaa tatcattagc
    54121 atgatttatt atagctttga tatctgaatt ttctgttttg atacctggtg ctacagcaac
    54181 aacatttgct gatggagttt taataaaatc attggtatca gtatctagcg taccacggac
    54241 atctgcataa gcttttcctt gagagagggc ttgaacgata cgtgttttac cgatagttcc
    54301 attagtgtat tgatgattat gtcctgcaaa aataatatca atgctatgtt cgggatagat
    54361 ttggttcact ttttccataa ccgtagccat ttcatgatca acaacaccat ctttacttgt
    54421 tgcaggaata tgagctaaaa ccacaatagc atgaacatgt tgttcttgca gttctttagc
    54481 atatttggca atggtttcag ctacatctaa aaattgatag tgttcatagt tttgctttaa
    54541 aacgagattt ggaatctctg tagtcacaac accaatgaag ccaatcttaa cgattttgtc
    54601 attaatggct atgtctttta tagcataagg tttccaacca taggggatat cattagtttt
    54661 tttatcaatg acattagcaa tgacgatggt ttgatgcgaa gcttcgtgct catattgttt
    54721 ggtgatatca ttaattgttg attcaggatc aggcgcttga cctgtcatga tacggttaaa
    54781 ttcatctagt ccttcgtcaa attcatgatt accaagagtg ccatattcaa atttcatttt
    54841 gttaaagact tttacagtag gctcatcttg taaaagtgca gagttagcag gactggctcc
    54901 gaccatatct ccagcttgaa cacgtatact tgttccgtct tgatttgctt gcttgaagtc
    54961 tatctcagcg tcatccatat aagcacctaa ttgagcagcc gtcccagcat ttggtatttt
    55021 accacttggt gtgtaagctg ttccggtatt atcaagagcg ccgtgaaaat cattgacgcc
    55081 aaggaattgc acatcaactt gatctgcttg aacatctgta actaatagag taaaactagt
    55141 caggata
//
LOCUS       DAUDYX010000006        49694 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.006, whole genome shotgun sequence.
ACCESSION   DAUDYX010000006 DAUDYX010000000
VERSION     DAUDYX010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49694
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.006"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..1270
                     /gene="rocA"
                     /locus_tag="VHD02_000356"
     CDS             <1..1270
                     /gene="rocA"
                     /locus_tag="VHD02_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator RocA"
                     /protein_id="HEQ5496991.1"
                     /translation="SAIPLRLKNIFYLSLSMVLFQVVFWAFFPDHFILDVVMLAQFLF
                     FALIALYYGKSIKAKFLMFYAFFPLVSISLVKRFIVFFVMPLFGMPYSVVKHNTLLIY
                     SITCFSIFLIYRCIQVFHFDFSTWRQYFQSHRASKLLVFTNSSMALYYLCVQGIDVMS
                     PSLSGLATTTARSIIVLFYFILFLTLLIHLERYVKQNSIEAIVQQKEYRELINYSQHL
                     GLLYQDIQELRQLLTTVSSRLKIGIEQNDISIVRLTYEGILNAEKNNAKDDRLDLTCL
                     DKLQVEAIRHIVLAKLIEAKNKKLKVEVSIPNCIATFFLEVVDFTKLLSFLLDNAIEM
                     SLETKQPCLSIAFLDQNHKLVIVIQSSTKQGQDDSQSVFAIPALKKRDDWQFDLRNVT
                     TILNRYDYLTISSQIHDGILTQLIEIAKPD"
     gene            complement(1371..4076)
                     /locus_tag="VHD02_000357"
     CDS             complement(1371..4076)
                     /locus_tag="VHD02_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEQ5496992.1"
                     /translation="MATKKVHIISHSHWDREWYMAYEQHHMRLINLIDDLLEVFQTNP
                     NFHSFHLDGQTIILDDYLKVRPEREPEIRQAIASGKLRIGPFYILQDDFLTSSESNVR
                     NMLIGKEDCDKWGASVPLGYFPDTFGNMGQTPQLMLKAGLQAAAFGRGIRPTGFNNQV
                     DTSEKYSSQFSEISWQGPDDSRILGLLFANWYSNGNEIPTTEAEARLFWDKKLADAER
                     FASTKHLLMMNGCDHQPVQLDVTKAIALANQLYPDYEFVHSCFEDYLADLADDLPENL
                     STVQGEITSQETDGWYTLANTASARIYLKQANTRVSRQLENITEPLAAMAYEVTSTYP
                     HDQLRYAWKTLMQNHPHDSICGCSVDSVHREMMTRFEKAYEVGHYLAKEAAKQIADAI
                     DTRDFPMDSQPFVLFNTSGHSKTSVAELSLTWKTYHFGQRFPKEVYQEAQEYLAGLSQ
                     SFQIIDTSGQVSPEAEILGTSIAFDYDLPERSFREPYFAIKVRLRLPITLPAMSWKTL
                     ALKLGNETTPSETDSLYDDSNQCLENGFLKVMIQTDGRLTITDKQSGLIYQDLLRFED
                     CGDIGNEYISRQPTHDQPFYADQGVTHISVRSNTIQVAELEIQQTFTIPVSADDRLQA
                     EMEAVIDITERQARRSQEKAELTLTTLIRMEKNNPRLQFTTRFDNQMTNHRLRVLFPT
                     HLKTDHHLADSIFETVKRPNHPDATFWKNPSNPQHQECFVSLFDGENGVTIGNYGLNE
                     YEILPDTNTIAITLLRSVGEMGDWGYFPTPEAQCLGKHSLSYSFESITKQTQFASYWR
                     AQEGQVPVITTQTNQHEGTLAAEYSYLTGTNDQVALTAFKRRLADNALITRSYNLSND
                     KTCDFSLSLPNYNAKVTNLLEKDSKQSTPSQLGKAEILTLAWKKQ"
     gene            complement(4091..5377)
                     /locus_tag="VHD02_000358"
     CDS             complement(4091..5377)
                     /locus_tag="VHD02_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEQ5496993.1"
                     /translation="MTRPIPTSVSQFMAKVESLCGDQHPDWALNFKTSFTNTLETTLK
                     TYEDGTSFLLTGDIPAMWLRDSTAQIKPYLFLAKEDEEIRKIIAGLVKRQFRYICIDP
                     YANAFNEEANEKGHQTDHTQMNPWIWERKYEIDSLCYPIQLAYLLYRETGSTDQFNDD
                     FHRGVELILDLWTVEQDHAQSPYLFERDTWRKEDTLTHAGKGSPVAPTGMTWSGFRPS
                     DDACQYGYLIPSNMFAVVVLSYLEDLYNNLFHNEPIATRAKQLKEAIQSGIADHALVQ
                     NSKGETIYAYEVDGLGQFSIMDDANIPSLLAAPYLGFCAKDDPIYLATRRTIFSQENP
                     YYYQGNAAAGIGSSHTPENYIWHIALALQGLTALDQDSKKEMLDLLVATDAGTHLMHE
                     GFDVNDPYQYTREWFSWANMMFCELLLDYLGFSIAP"
     gene            5510..6550
                     /locus_tag="VHD02_000359"
     CDS             5510..6550
                     /locus_tag="VHD02_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ5496994.1"
                     /translation="MTKLLYKKIYHFIKEQIASGRLQIGDRLPTEKELSEQFSVSRIT
                     SKRALVELEQEGLITRSRGKGSFVAKKQVTSPEANKDLLLILPFASDYELGDYAKGIM
                     TSIAETGYRLMVQLASTVRLDTLSDYAGIIYYPEDVNHSIDFLFYCDHHHIPLVLLDK
                     SLDLFQFPAVVADNKGGAYQLTQHLIEQGCDQIWFVATGSFGEVSSVRDRYLGYLAAM
                     AESSLKSHYFPKTETETTDAYLDRLVKTLSETTATKRGLVVENDWLAIQLIQKITQVG
                     LSIPDQVAIVGFDNSQASRLLSPKLSTAAQDLYQMGQEAAKVLLQIIHEPDQAVVSCQ
                     LPVQVFIRESSH"
     gene            6601..8295
                     /locus_tag="VHD02_000360"
     CDS             6601..8295
                     /locus_tag="VHD02_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase"
                     /protein_id="HEQ5496995.1"
                     /translation="MTIYHALKDYQEVMTRGDYLVFDTPLTCRFIGHFFRFENQKALL
                     AELATSKYFQWIEEGQAEVTMKHFFNRQLAKDAFTLKVSEDKEIIIESQNLRGFRYAQ
                     EALLKVMTFKGDKLYLPIVSVKHSPSFPMRGIIEGFYGTPWTREERIDCLRFIGNKRM
                     NTYMYAPKDDDYQRKLWRDLYPEDWVTYFKELLAVAKEEGIDFWYMISPGLDFDYTKE
                     ADYQLLYQKLQQLLALGVCHFGLLLDDIDYQIVDAVERRFKKTAYAQAHLATEVHHFL
                     NQQHAAPELVICPTEYDNHHDSIYLQELSERIPKEVAFFWTGPSTLASQISQADIETM
                     AAVYQRPIIIWDNIPVNDYQKDPERLFLTPFANRSPFLCQPDYQVKGIVSNPMISWEL
                     SKLTLTDMSHYLWDANRYQPSHSWLETLTDYTEDTELALALQAFAWHNGNRHLHRDLP
                     FEVEEALLAKDVSTLSAWVAELVECVNTLRKLDKPAFQQAIAPWFERVAKDQVFWQAM
                     LNQEPQLETLYADLQEDKHRIGSDIPSRYYRIYYQQQDKLTANQGQVTQARPEDYA"
     gene            8320..9762
                     /locus_tag="VHD02_000361"
     CDS             8320..9762
                     /locus_tag="VHD02_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEQ5496996.1"
                     /translation="MLAFPKEFWWGGATSGPQSEGRFAKQHRNLFDYWYEEEPDLFYD
                     YVGPDTASDAYHQIESDLTLLASLGHNSYRTSIQWTRLIDDFEQATINPDGLAYYNRV
                     IDACLANGIRPVINLHHFDLPIALYQAYGGWESKHVVDLFVAFSKVCFQEFGDRVKDW
                     FVHNEPMVVVEGSYLMQFHYPAIVDGKKAVQVAYNLALATAKVIQAYRQGPAELSDGR
                     IGTILNLTPAYPASQSEADMAAAHFAELWNNDLFMEAAVHGKFPEELVAVLKKDGVLW
                     QSTPEELALIAENRVDYLGLNFYHPKRVKAPDVIPVISPSWSPEWYYDPYLMPGRRMN
                     VDKGWEIYPEAVYDIAIKMRDHYDNIPWFLSENGVGISGEERYRDETGQIQDDYRIQF
                     LKEHLTYLHKGIETGSNCFGYHVWTPIDGWSWLNAYKNRYGLVENNIHTQVRRPKASA
                     YWFKKVATHNRLISLEVMEEFGGSASHLSD"
     gene            complement(10373..11251)
                     /locus_tag="VHD02_000362"
     CDS             complement(10373..11251)
                     /locus_tag="VHD02_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEQ5496997.1"
                     /translation="MSLLCIDIGGTSLKFALCHNGQLSQQSSFPTPSSLEKFYQLLDQ
                     EVARYSAYHFSGIAVSAPGAVNKEKGIIEGASAIPYIHHFKIQEVLEERLHYPISIEN
                     DANCAALAEATLGAGKGASSLAMLVLGTGVGGSLVIDGKIYHGAHLFGGEFGFMIMND
                     RYQTFSQLGTVVNMAKRYSAIVNNGKDYTGKAVLALAEQGDHLAIKERQVFLQSLAIG
                     IFNIQHAFDPQLILIGGGVSQADFLLSAIEAELDKLYQAVEISDLRPQLAICHFKNEA
                     NLLGAAIDFYQEHKEA"
     gene            11447..12397
                     /locus_tag="VHD02_000363"
     CDS             11447..12397
                     /locus_tag="VHD02_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004219264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ5496998.1"
                     /translation="MTRQTNKKTSFWKNVIKYRALLLMVLPGFIWFIFFFYIPVLANV
                     VAFKDFHYSAGGFMESLKESPWVGLANFKYLFASKDAWLITRNTIAYNVIFLLFNVFF
                     AIAFAIIMSELRNKRTVKVYHTMSLLPYFLSWVVIEYFVSAFLNTDKGFINQLLTGSG
                     ADPIKWYSNPTWWPLILLFMSVWKGLGYNSIIYYASVKGISDTYYEAAMVDGASKWQQ
                     IRNITIPQLLPMMSILLIINIGNIFKSDFGLFYVIPKNSGPLYDVTSVLDTYVYNALT
                     ATGDIGMASAASLYQSVVGTSILLVTNAIVRRMDPDAALF"
     gene            12407..13333
                     /locus_tag="VHD02_000364"
     CDS             12407..13333
                     /locus_tag="VHD02_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEQ5496999.1"
                     /translation="MEKKKKVEKVNVRTFDRKTNAIFNILIGLFAISCIIPFIFVIII
                     SFTDESYLINHGYSFFPDVWSTKAYQYIFQGAMSHRIMRSFGISVFITVVGTFINTTM
                     TSTYAYAISRPYFPYRRFFTVYALITMLFAPGMVANYLVVSNLLHLKDTVWALILPMA
                     LGPFGILVMRTFFKKTVPDSIIESARMDGASEWMIFMKIVLPLAVPGIATISLFSALT
                     YWNDWFNALLYVQSENLYPMQYLLMKIQSNLQALAQNAGMSAQMADSLASLPKESVRM
                     AIVVIATLPIALTYPFFQKYFVGGLTIGGVKE"
     gene            13414..14859
                     /locus_tag="VHD02_000365"
     CDS             13414..14859
                     /locus_tag="VHD02_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ5497000.1"
                     /translation="MKKWQKIVCVTGTVLAASSLAACESKSASKDSDVKLLMYQVGDK
                     PDNFDELMTIANKRIKEKTGATVDLQYIGWGDWDDKMSTIIASGENYDIAFANNYVVN
                     AQKGAFADLTTLMPKYAKKTYKNLDPAYIKGNTIDGKLYAFPVDANVYAQQMLSFNKE
                     LVDKYGLDISNIKSYADAENVLKQFHEKEPNTAAFAIGQVFSMSGDYDYPLTKNQPFA
                     VKIDEGKPTIINQYEDESFKNNLRLMHKWYKEGLIPTDAATNTEGYPLEGNTWFMREE
                     TQGPMDYGDTILTNAAGKDIVSRPLTKPLKTTSQAQMANFVVSSVSKNKEKAVEVLSL
                     LNSDPELLNGLVYGVEGKAWEKIGDKKIKLLDGYQPKMHMGAWNTGNNKILYTQESVT
                     DDMIAKRDQSIKDAKESPILGFTVDTKVIKTELSNISNVMNRYKASINTGTVDPDEAL
                     PKLLADLKGAGWDKVQKEVQKQLDDFVAKDK"
     gene            15165..15770
                     /locus_tag="VHD02_000366"
     CDS             15165..15770
                     /locus_tag="VHD02_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEQ5497001.1"
                     /translation="MNGIEKTCFLIWKMIQLTLIFHLLSLAGLIVFGVGPAWQTIVTL
                     FLKTSQEENHYSLKSAVQLWKSYFKEANLRFGLFALTFLFLTFNLHWAVQFPSLFWFT
                     VSFLIVIAMVWLTMTYLYMVFYAVSYEISLWNNMKLAFISVFLSFKSFLLMLSVLLGI
                     ILVTWQYKGLYLFLTFGALVFCLDFVTKANRRLVDGVIDEC"
     gene            15760..17484
                     /locus_tag="VHD02_000367"
     CDS             15760..17484
                     /locus_tag="VHD02_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ5497002.1"
                     /translation="MSVKTLGRYVTNHFKQGFLINRLMKLYSLLIVSFFTLAAIGLSS
                     YSISSTYKRVDAEAQMRLEETLGRLQSQNDITLRVLDQLVGRSEDYHNLYQYMTLTPN
                     QYFSNVFEDWEKGKDTVLFSEEVRRLFDLYPDVTSMTLLLEDSSAYLYADRVIKTGRL
                     LYSKPEKVTGNVLVRSIRNPESGDVTGRLYLTFDKPPTLVETQQDHYLATFAFDYYGR
                     KLFHQGGRRFPHLEAEVKKAIKADRAADLSNLSKAYRMQYNRSGDLLAYVAVRKSYLL
                     AEAVRTVFVHGLVSLLLAWLLLQLLFRVFRNYIQQVSEITDTVEMVAAGDLSLTIDNS
                     HMELELYHISEAINQMLASIKAYIDEVYVLEVEQRDAQMRALQSQINPHFLYNTLEYI
                     RMYALSCQQEELADVIYAFASLLRNNISQDKMTTLKEELAFCEKYIYLYQMRYPDSFA
                     YHVKIDESIADLAIPKFVIQPLVENYFVHGIDYSRNDNALSIKALDETDHLLIQVLDN
                     GRGISQERLADMEKRLQEHQTTGNSSIGLQNVYLRLFHHFRDRVSWSMAKEPNGGFII
                     QIRIRKDA"
     gene            17484..18968
                     /locus_tag="VHD02_000368"
     CDS             17484..18968
                     /locus_tag="VHD02_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ5497003.1"
                     /translation="MYKVLLVDDEYMILQGLTMIIDWQALGFEVVQTARSGKEALAYL
                     TQYPVDVMISDVTMPGMTGLDLIEAAKTYHPQLQTLILSGYQEFSYVQKAMELETKGY
                     LLKPVDKAELQAKMKQFKDCLDAQQAESIRQEAYHDSLLTLWLTDELSEKEFQQLSQG
                     LPAAALIGFTVLYLDCQEWQAAIDAYFEQEGQPFYLKKEEDKKVYLAVLLGKTDQAKA
                     FTYDLQKRFSRNMHQLILGETVDDWENVYESYNQVLKSLFYNCEVSPIRAKDKSKIEL
                     PESRLQFFAFNKALMIGDEPTILSKLEAIFDEMKTLNFSPEDVKHVSFLLFSDIYRQF
                     PILDKMTYLSMVKTIHDSQSIDRILRELKKVLDVTNQNNSPEKRYSDLVSETIDCIRK
                     EYHQELTLKAIADRLHVNGVYLGQCFKNETERSFTQYLNHVRIQKAQQLLLYTNQSIN
                     EIAYETGYNTNHYFIKMFKKLNGLSPKEFRDRYKDNYQAIKGDQ"
     gene            18898..22404
                     /locus_tag="VHD02_000369"
     CDS             18898..22404
                     /locus_tag="VHD02_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEQ5497004.1"
                     /translation="MVYRQKSSEIVTRTITKPSRATSNIKQEIDMTPDSKEQTVTGYQ
                     YHYIDQEGRKQPFNQGWRFLMADVACAQAPSFDDSNWQVIHLPHDFSLTQPYTRNGEA
                     ESAYKLGGVGWYRHYLVLDEVLAGCHVAITFEGSYMETEIYVNGQFIGKHLNGYQEFT
                     YDISDVVTFGAENLLAVRVENKVPSSRWYSGSGLYREVSLSVLPQLHFVADQVAMTLA
                     DTAVQEKGQQKVDLRFALNQPIQTCHYQLSLCLWEQSHCSKDKKLLYQETEVPLADLA
                     FQRQYGLTLSLEELQLWSPDNPHLYDLELTLYYQGQVIDCFCLETGFRQLTFTANQGL
                     FVNGRAVKLKGVCLHHDQGGLGACAYEDALARQLVLLKDMGANTIRSTHNPSSPKLRQ
                     LANRLGFFVIEEAFDTWTYAKNGNVNDFSNYFHQTIGTENANYLQRVRSPETSWAQYS
                     IEAMVWSAKNDPSVLMWSIGNELMEGFSADVSHYPELTRQMCQWIAAIDTSRPITFGD
                     NKLKEADFCWHEEVSQMATLLSQLDHPQGLIGLNYANGKDYDKLHEEHSDWLLYGSET
                     VSAITSRAYYKETKKVLDSGYHLTSYDHAKVDWGAFASQAWYDTITRDFVAGECVWTG
                     FDYLGEPTPWNKIDSGVVGLWPSPKNAYFGILDTAGFPKDSYYFYQSQWVQGQTTLHL
                     LPVWQKDQLCFDEQGLVEVVVYSNAASVQLMFEDEQGNLTDYGRKAFHTYSTPTGHTY
                     QLYQGADAAKNPHENLYLTWRVPYQKGLLRAVAYDISGKSIPKTSGRSQVRTYGSVAK
                     LSWKAFEAPIDAPWELLYLDLSLLDSQGELVSHAQDWLKVQVEGPARLLALDNGNPTD
                     HTPYQEPLRQAYGGKLLAILALTGEAGHIKVTAKASQQLSSVFQTQVACRAPKEHLVT
                     LPLEKYQFKKSRLTSQQEIGQSCLKTTQFLSVLENKDTQQKAGKASTPHILITSYQHY
                     LSLPLQVCSQAFDFKKGDDIFLPQWLQVKDAQGSFVKKAFPMTWCLKEKIFAKPILVT
                     GYVSCFGQSIPVEARLNLAQASKRRHQDLSLVAKQSLATSQKRLTYCYQYDTVQAIGA
                     ISLQNHTGTSLSVSVAIGYGSQSSIKQETLMLADSHLSQLEEVIFFDDILTVLYLAVI
                     LEVIEGPDLTEDSIQIGLWDLA"
     gene            22542..23420
                     /locus_tag="VHD02_000370"
     CDS             22542..23420
                     /locus_tag="VHD02_000370"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEQ5497005.1"
                     /translation="MSERLSGHTLLVSLLATPIRHSLSPKMHNEAYAKLGLDYAYLAF
                     EVGTEQLADAVQGIRALGIRGSNVSMPNKEAILPLLDDLSPAAELVGAVNTVVNKDGK
                     GHLVGHITDGIGALRALADEGVSVKNKIITLAGVGGAGKAIAVQLAFDGAKEVRLFNR
                     QATRLSSVQKLVTKLNQLTRTKVTLQDLEDQTAFKEAIRESHLFIDATSVGMKPLENL
                     SLITDPELIRPDLVVFDIVYSPAETKLLAFARQHGAQKVINGLGMVLYQGAEAFKLIT
                     GQDMPVDAIKPLLGDE"
     gene            23655..24410
                     /locus_tag="VHD02_000371"
     CDS             23655..24410
                     /locus_tag="VHD02_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEQ5497006.1"
                     /translation="MVTKEVGHDFLARLGKKRLRPGGVEATNWLIEQGQFSQDCHVLE
                     VACNRCTTAIELAKTFGCQIDAVDLNPKVVKEAQERVAVEGLTRHITVSQANALSLPF
                     PDNHFDIVINEAMLTMLNAQAKEKALNEYWRVLKPGGRLLTHDVAYEDPKTRQVLDEL
                     RQTIHVNVAPLSLTGWQALYQEHGFSQVTYHHGKMSLMTPRGMIKDEGLMNTLNIMRR
                     GLKKENRQQFLKMRRFFTRTGKNLCYIAVKSVK"
     gene            24463..24801
                     /locus_tag="VHD02_000372"
     CDS             24463..24801
                     /locus_tag="VHD02_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002265614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEQ5497007.1"
                     /translation="MSYINNIEHAKVLDLTQEVMIEQDQMLSRTLVQRQDLGITVFSL
                     DKGQEIGRHSSPGDAMVTILSGLAEITIDQETYRVAEGQTIVMPAGIPHALYAVEAFQ
                     MLLVVVKPEV"
     gene            24803..25108
                     /locus_tag="VHD02_000373"
     CDS             24803..25108
                     /locus_tag="VHD02_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEQ5497008.1"
                     /translation="MIENLSQLVQYHDHQVASRSLSRLLGLSQSIVLYAMAQGESISQ
                     ETSPKDKVILVLEGQLIFDLEDQKQVLTQESLIAIPAQKVHHLEAKTDCKLLQVLLD"
     gene            complement(25544..26617)
                     /locus_tag="VHD02_000374"
     CDS             complement(25544..26617)
                     /locus_tag="VHD02_000374"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEQ5497009.1"
                     /translation="MPQTLHVHSRVKDYDILFTDHVLKTLADCLGERKQRKLLFITDQ
                     TVYHLYQTLFEEFAQQYNAFVHVCPPGGQTKSLERVSAIYDQLIAENFSKKDMIVTIG
                     GGVVGDLGGFVAATYYRGIPYIQIPTTLLSQVDSSIGGKVGVHFKSLTNMIGSIYPPE
                     AIIISTTFLETLPQREFSCGISEMLKIGFIHDRPLFQQLRDFQKETDKQGLERLIYQS
                     ISNKKQIVEQDEFENGLRMSLNFGHTLGHAIESLCHHDFYHHGEAIAIGMVVDAKLAV
                     SKGLLPKEDLDSLLQVFERYQLPTTLERADVSATSLFDVFKTDKKNSEQHIIFILPTE
                     TGFTTLAINKDDHQFVEKLDSLL"
     gene            complement(26673..27425)
                     /locus_tag="VHD02_000375"
     CDS             complement(26673..27425)
                     /locus_tag="VHD02_000375"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEQ5497010.1"
                     /translation="MIDIMSDFQNKTCSKNNFIVGPCSIESYDHIRLAASSAKKLGYN
                     YFRGGAYKPRTSAASFQGLGLQGIRYLHEVCQEFGLLSVSEIMSERQLEEAYDYLDVI
                     QVGARNMQNFEFLKTLSHIDKPILFKRGLMATIEEYLGALSYLQDTGKSNIILCERGV
                     RGYDVETRNMLDIMAVPIIQQKTDLPIIVDVSHSTGRRDLLLPAAKIAKAVGANGIMM
                     EVHPDPDHALSDAAQQIDYKQLEQLGQELWQD"
     gene            complement(27665..27735)
                     /locus_tag="VHD02_000376"
     tRNA            complement(27665..27735)
                     /locus_tag="VHD02_000376"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27701..27703),aa:Thr,seq:ggt)
     gene            27902..28156
                     /locus_tag="VHD02_000377"
     CDS             27902..28156
                     /locus_tag="VHD02_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEQ5497011.1"
                     /translation="MSYEKEFLKDFEDWVKTQIQVNQLAMATSQEVAQEDGDERAKDA
                     FIRYESKLDAYEFLLGKFDNYKNGKAFHDIPDELFGARHY"
     gene            28177..28572
                     /locus_tag="VHD02_000378"
     CDS             28177..28572
                     /locus_tag="VHD02_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEQ5497012.1"
                     /translation="MAKQKNRKKALKLEMKRNGLLKKAGQVFDKAVESVETAVDKTIS
                     AGKNLVEKGSQTVENLTASKERLTLDEFAGQPVVAGIRSDLVETLYAEGIHSAQAFKE
                     WTEKDLLALKGIGPATVKKLVENGASFKK"
     gene            28857..29176
                     /locus_tag="VHD02_000379"
                     /pseudo
     CDS             28857..29176
                     /locus_tag="VHD02_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264372.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            29173..29736
                     /locus_tag="VHD02_000380"
     CDS             29173..29736
                     /locus_tag="VHD02_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ5497013.1"
                     /translation="MNLIIIGAQASGKMTIGQEVARQTGMTLFHNHDSIDFVLRFMPW
                     SQESTALIERIRFAFFETFAKTGQDMIFTIVIDFNDPNDVAMLEKIQAVFQSYDQEVL
                     FVELKTDIEERLKRNRTENRLKHKPLKRNIEWSEQDIQSTMAYAVFNPEEPPKTLTHY
                     QKINNTQLTAAETAQLIIQKMTHIKEN"
     gene            29738..32386
                     /locus_tag="VHD02_000381"
     CDS             29738..32386
                     /locus_tag="VHD02_000381"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEQ5497014.1"
                     /translation="MTELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIPP
                     PNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQGI
                     SRYDLGRDKFLDKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVFV
                     DLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALQVATT
                     RPETMFGDVAVAVNPEDPRYKDLIGKNVILPIVNKLIPIVGDEHADPEFGTGVVKITP
                     AHDPNDFEVGQRHNLPQVNVMNDDGTMNELAGDFAGMDRFEARQATVAKLEELGALVN
                     IEKRVHSVGHSERSGAVVEPRLSTQWFVKMDELAKQAMDNQETDDRVDFYPPRFNDTF
                     LQWMENVHDWVISRQLWWGHQIPAWYNAEGEIYVGEEAPEGDGWTQDEDVLDTWFSSA
                     LWPFSTMGWPDTDVEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVLI
                     HGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADSLRWFLSNGSAPGQDVRFSYEKMDAS
                     WNFINKIWNISRYILMNNEGLTLEEAESNVAKVAASEAGNVTDQWILHNLNETIAKVT
                     ENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEAEKVITRSVLLYTLDKIL
                     RLLHPIMPFVTEEIYAQYAQGSIVTVDYPVVRPAFENEAAHKGVESLKDLIRAVRNAR
                     AEVNVAPSKPITILVKTADSELEDFFTSNVNYIKRFTNPEKLEISSAIAAPELAMTSI
                     ITGAEIYLPLADLLNVEEELVRLDKELAKWQKELDMVGKKLGNERFVANAKPEVVQKE
                     KDKQADYQAKYDATQERIAEMKKIKS"
     gene            32540..34948
                     /locus_tag="VHD02_000382"
     CDS             32540..34948
                     /locus_tag="VHD02_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HEQ5497015.1"
                     /translation="MRMILAHYDCKKDKKQSLDEHLWHVACSSRQEASIIGQGDVLFL
                     IGLYHDLGKADRTFQDKLLNNPNRHVDHSYAGAKYLCSIIGPHLKNRGVDKNERMTFN
                     EMVGYVISAHHGMYDLCYYFDDAEYYGFNKFKNRINRDLDGYHYHEDIKGYALKLEKK
                     LCDYGYKDLRELIDKAFDNYQQAMSSLNWQDKSEWDYYQSCMVRLYLSLLKNADILDT
                     VNAYGLKISPMDKTERSFLKHSYLAAIEQKYASFGQPNNQLNTIRTEIAERVKERGKR
                     DSKGIYRLDLPTGAGKTNLSMRYAFHQLVHHDKSRFFYITPFLSVLEQNASEIRKVTG
                     DLGVLEHHSNVVKQANEDDDDKDSLLSAYLSDSWDSQVVLTSMVQFFQTLFKTKSANL
                     RRFSSLINSVVILDEVQSLPIEVTTLFNLTMNFLNKVMDTTIVLCTATQPAYDSSEID
                     HRICYGGNLGELAEIVELTIEEKQIFSRTELRKFDDSDQKVHLTDVINLILGEENSVL
                     AIFNTKKTVHNCYTMLKDITDRPVYQLSTNMCAQHRLDLIAKIKTELQNNIPIICIST
                     QLIEAGVDVDFHRVIRSYSGIDSIVQAAGRCNREGKRDKGQVTLVNLTNEEENISRLT
                     EIKAKKEATESILHKIGSPIDISTLNRDFFEYYYANNQGLMDYPLEDNLSIYDYLSLN
                     IYQTANKKFKGKLKQAFKTAGAKMNLINNDMIGILVPYGEAEKKLAYLEELGMSHFLS
                     AEDYQTIKSLLKELQPFTVNVRENDPLFETTKSYLNGQILVLTSEYYDTERGVKYDSA
                     SFYF"
     gene            35081..35809
                     /gene="cas5c"
                     /locus_tag="VHD02_000383"
     CDS             35081..35809
                     /gene="cas5c"
                     /locus_tag="VHD02_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HEQ5497016.1"
                     /translation="MYRSRDFYVRVSGQRALFTNPATKGGSERSSYSVPTRQALNGIV
                     DAIYYKPTFTNIVTEVKVINQIQTELQGVRALLHDYSADLSYVSYLSDVVYLIKFHFV
                     WNEDRKDLNSDRLPAKHEAIMERSIRKGGRRDVFLGTRECLGLLDDISQEEYETTVSY
                     YNGVNIDLGIMFHSFAYPKDKKTPLKSYFTKTVMKNGVITFKAQSECDIVNTLSSYAF
                     KAPEEIKSVNDECMEYDAMEKGEN"
     gene            35809..37704
                     /gene="cas8c"
                     /locus_tag="VHD02_000384"
     CDS             35809..37704
                     /gene="cas8c"
                     /locus_tag="VHD02_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HEQ5497017.1"
                     /translation="MDFFTSLLKTYEKAELADLVDHQKRSNEPVLLPIYHTSLKSNGK
                     NIISVKLDKDGQFHKAEFMADKQMIIFPVTADSVARSGSHPAPHPLVDKFAYYSAEMG
                     QSQYDSFHKQLNNWIDYCEEGDVKRFLTIVQQFILKPEFLTLILDSLIGPDYQNNQLK
                     VTFCDATGKEKLIDLSACFLEFSIDQFQGFKNESVSTFKTLHQSYISFVEANREKLGI
                     CNISGREEQLTDKHRGLMGNAKIISVSNKREAYKGRFREREDVFSVGYETSEKIHLML
                     KYLLENKNTSTWLGSSQYLINWFSDDLTNDSRLDIVSPIFDDGLEEDDDDDTPPVITL
                     ATEDNKRIGKSFIKGQKLFANDATYYVAILNKTSNGRIALKYFRQLQASQLLTNLNKW
                     QETYSWESRSKFGKSRLRTPTFHDILNVSYGVDRDRFLELDNDNFKSDQIQKLVASLI
                     DGKPMPQSIVKKLGNNVKERHRYRKHWYQVEQVCLAILHKQNGEEFSPMLDHTNQNRS
                     YLFGRLLAIFELIETLRYGLDGNNNDRITNAERYWTAYTGQPTKLMMLLENKIKPYEE
                     PLKLNRRGSWMKLEKEKEEILELLNPLLETETMGKPLDYRFIFGYYAEKNYYYTKQNT
                     EVTESEE"
     gene            37709..38557
                     /gene="cas7c"
                     /locus_tag="VHD02_000385"
     CDS             37709..38557
                     /gene="cas7c"
                     /locus_tag="VHD02_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HEQ5497018.1"
                     /translation="MLEHKIDFMVTLEVKEANANGDPLNGNMPRTDAKGYGVMSDVSI
                     KRKIRNRLQDMGKSIFVQANERIEDDFRSLEKRFSQHFTAKTPDKEIEEKANALWFDV
                     RAFGQVFTYLKKSIGVRGPVSISMAKSLEPIVISSLQITRSTNGMEAKNNSGRSSDTM
                     GTKHFVDYGVYVLKGSINAYFAEKTVFSQEDAEAIKEVLVSLFENDASSARPEGSMRV
                     CEVFWFTHSSKLGNVSSARVFDLLEYNQSIEEKSTYDAYQIHLNQEKLAKYEAKGLTL
                     EILEGL"
     gene            38559..39233
                     /gene="cas4"
                     /locus_tag="VHD02_000386"
     CDS             38559..39233
                     /gene="cas4"
                     /locus_tag="VHD02_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HEQ5497019.1"
                     /translation="MVYAEDDYLMLSGIQHFQFCKRQWALIHIEQQWLDNEATAHGQV
                     LHTKADNPYIKEKRKELLVSRAMPISSAELGLSGIMDVVEFYKDDQGVSLRGKRGKWL
                     PKVVEYKRGKPKKDTRDIVQLVAQTMCLEETLDCDINEGCLYYHSVNQRVIVPMTSAL
                     RQEVKELAAEMHEVYQSQMLPKAAYFKNCQLCSLVDICKPRLSKKTRSVSRYINEAMT
                     SEEMDL"
     gene            39230..40255
                     /gene="cas1c"
                     /locus_tag="VHD02_000387"
     CDS             39230..40255
                     /gene="cas1c"
                     /locus_tag="VHD02_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HEQ5497020.1"
                     /translation="MKKLLNTLYLTQEDFYVTKEGDNIVIKQEGKVLKRFPFRIIDGI
                     VCFSYLGVSPALVKLCTENQINLSFHTPQGRFCGRYIGSTNGNVLLRREHYRLSDREE
                     SLEYAKRFILAKISNSRKYLLRFKRDHRQQIDTKLFEAVNDELIWALEMVQAADNKDS
                     LRGIEGQAANQYFRIFNDLVLTDKKTFYFQGRSKRPPLDCVNALLSFGYSLLTFECQS
                     ALEAVGLDSYVGFFHTDRPGRASLALDLVEEFRSYIVDRFVFSLINKGQLQKKHFEVK
                     ENGSILLTENGRAIFIDLWQKRKHTEVEHPFTKEKVKLMLLPYVQAQLLAKAIRGDLE
                     SYPPFMV"
     gene            40266..40559
                     /gene="cas2"
                     /locus_tag="VHD02_000388"
     CDS             40266..40559
                     /gene="cas2"
                     /locus_tag="VHD02_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEQ5497021.1"
                     /translation="MMVLVTYDVNTETPAGRKRLRHVAKLCVDYGQRVQNSVFECSVT
                     PAEFVDIKHRLTQIIDEKTDSIRFYLLGKNWQRRVETLGRSDSYDPDKGVLLL"
     repeat_region   40708..41140
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=40708..40739
                     /rpt_unit_seq="gtctcacccttcatgggtgagtggattgaaat"
     gene            41300..42010
                     /gene="ccdA2"
                     /locus_tag="VHD02_000389"
     CDS             41300..42010
                     /gene="ccdA2"
                     /locus_tag="VHD02_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEQ5497022.1"
                     /translation="MILSLVLMVSVFGAGLLSFFSPCIFPVLPVYLGILLDADDSKTI
                     TIFGKKLYWYGIVKTLAFIFGLSTIFVILGYGAGFLGNILYAVWFRYLLGALVIILGI
                     HQMGLITIKSLQFQKSLTFHNNKNRNGLFNAFILGLTFSFGWTPCVGPVLSSVLALVA
                     SGGNGAWQGGVLMIIYTLGLGIPFLLISFASGIVLKQFNKLKPHILLLKKVGGVLIIV
                     MGILLMTGTLNNLAQLFG"
     gene            42023..42646
                     /locus_tag="VHD02_000390"
     CDS             42023..42646
                     /locus_tag="VHD02_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEQ5497023.1"
                     /translation="MKKGLLVTTGLACLGLLTACSTQDNMAKKEITQDKMSMAAKKKD
                     KMSTSKDKSMMADKSSDKKMTNDGPMAPDFELKGIDGKTYRLSEFKGKKVYLKFWASW
                     CSICLSTLADTEDLAKMSDKDYVVLTVVSPGHQGEKSEADFKKWFQGTDYKDLPVLLD
                     PDGKLLEAYGVRSYPTEVFIGSDGVLAKKHIGYAKKSDIKKTLKGIH"
     gene            42689..43795
                     /gene="msrB"
                     /locus_tag="VHD02_000391"
     CDS             42689..43795
                     /gene="msrB"
                     /locus_tag="VHD02_000391"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEQ5497024.1"
                     /translation="MEHKWKLWLFIVGVVLLIGGASVLIQKGQAKAFSQGKPQLPAKK
                     VKSTKVDPNAPVDQKMIYLAGGCFWGVEEYFSQVDGVLDAVSGYANGRGDTTNYQLIH
                     QTGHAETVEVAYDANRISLKELLLHFFRIIDPTSLNKQGNDRGSQYRTGTYYTDKADL
                     AIIDEVFKEKAKDYKKKIVVEKAPLKHFIKAEDYHQDYLKKNPNGYCHIDINQATYPV
                     IDESKYPKPSATEIKAKLSADEYRVTQKNETEKAFSNRYWDSFDAGIYVDVVTGEPLF
                     SSKDKFESGCGWPSFSRPISPDVIRYKEDKSFNMTRTEVRSRSGDSHLGHVFTDGPKD
                     QGGLRYCINSLSITFIPKADMEAKGYGYLLSSVE"
     gene            43883..44623
                     /locus_tag="VHD02_000392"
     CDS             43883..44623
                     /locus_tag="VHD02_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEQ5497025.1"
                     /translation="MYSLLIVEDEYLVRQGIRSLVDFSQFKIDRVNEAENGQLAWDLF
                     QKEPYDIVLTDINMPKLNGIQLAELIKQESPQTHLVFLTGYDDFNYALSALKLGADDY
                     LLKPFSKADVEDMLGKLRKKLELSKKTETIQELVEQPQKEVSAIAMAIHERLADSDLT
                     LKSLAQQLGFSPNYLSVLIKKDLGMPFQDYLVQERLKKAKLLLLTSNLKIYEIAEQVG
                     FEDMNYFSQRFKQLVGVTPSQYKKGGQA"
     gene            44620..46353
                     /locus_tag="VHD02_000393"
     CDS             44620..46353
                     /locus_tag="VHD02_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ5497026.1"
                     /translation="MKRYPLLVQLISYVFVIVIALITTLGLLYYQTSSRNIRQLIERD
                     TRQSIRQSSQFIDAYIKPLKETTSVLAKNTEIQAFASQIHQENDKQVLQLMKMVLATN
                     SDLQAAVLVTKDGRTVPTNSQLTMKTSSDMMAEPWYKAAIDRQAMPVLTPARQLSLSS
                     KKEWVVSVTQEVVDAAGHNLGVLRLDIAYPTIKASLDQLQLGRQGFAFIVNDKHEFVY
                     HPKKSVYSSSKEMAAMKPYLAIQNGYTKDKTSFVYQKLIPNSQWTLVGVASLDQLHRV
                     QRQIFWSFLGTGLLACLICGFATVLALRRWIRPIQQLQQVILAIQKGDRQLRAQETGS
                     PELTDLAQQFNALLDQIDSLMVAVADKEKAIGQYRLQALASQINPHFLYNTLDTIIWM
                     AEFNDSKRVVEVTKSLAKYFRLALNQGNEYIRLADELDHVSQYLFIQKQRYGDKLSYE
                     VQGLDVYADFVIPKLILQPLVENAIYHGIKEVDRKGMIKVTVSDTAQHLVLTVWDNGK
                     GIEDSSLTNSQSLLARGGVGLKNVDQRLKLHYGEGYHMTIHSQSDQFTEIQLTLPKMH
                     ELMADDTQENE"
     gene            46426..47505
                     /locus_tag="VHD02_000394"
     CDS             46426..47505
                     /locus_tag="VHD02_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEQ5497027.1"
                     /translation="MSLASDYFSRQTPIVEKLMAYGFEKRNNGYFYNERFMEEEFEAQ
                     LRIDEAGNIWDRVIDCDLEEDYLPLQQAAWQGTYTGQVRAAYLELLERLSVACFEVTP
                     FQSMQANRLAKHITKEWSDPMDYPFEKHPDLATYRVGGKWYAMIFSLLADKLDQIPER
                     LVGQTCEVMTVKVNPKDLPQLLQQEGIYPAYHMSKKNWISIILDDKVTDDKLWTLVTQ
                     SRQLVNPNGLSNPNGPGYWVIPANLKYYDIDAEFAANDVILWTQKAGIAVGDYVLIYM
                     TAPVKSIRYACQVLETDIPNEGYRKNPNIDKLMRLRKCQQYKDGLLSFDLMKKHGVAA
                     VRGPRRLSPQLIAFLKEKEYLKENN"
     gene            47528..48244
                     /locus_tag="VHD02_000395"
     CDS             47528..48244
                     /locus_tag="VHD02_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEQ5497028.1"
                     /translation="MVEFVADQSFWELFPDAKLGVVLLKDVQNQAESPEDVKQLLADS
                     HELVKAYLTADNFSDNQVVQVYRKAYQHFKTKKGARSSIEALLKRVSNGQSIPSINPL
                     VDIYNAASLRFGLPVGAEDSDSFIGDLRLTTTDGGDDFYLIGDADNNPTLPNELCYKD
                     DIGAVCRCLNWRDGERTMVTEHTKNAFLIIEALDQEGQNRLQEALDFIEGSAKMYLHA
                     ITSVHVLDKDNPHVPLFTEN"
     gene            complement(48410..48883)
                     /locus_tag="VHD02_000396"
     CDS             complement(48410..48883)
                     /locus_tag="VHD02_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEQ5497029.1"
                     /translation="MNKKETRHQLIRSLISETTIHTQQELQERLQKNGITITQATLSR
                     DMKELNLVKVTSGNDTHYEALAISQTRWEHRLRFYMEDALVMLKIVQHQIILKTLPGL
                     AQSFGSILDAMQIPEIVATVCGDDTCLIVCEDNEQAKACYETLSHYTPPFFFSNK"
     gene            49025..>49694
                     /locus_tag="VHD02_000397"
     CDS             49025..>49694
                     /locus_tag="VHD02_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEQ5497030.1"
                     /translation="MIRREDYQYLRKLNDFRYFSIEQFDKIVGQMEFRKAKKDHILFF
                     EGDKRDKLFLVTSGYFKVEQSDQSGTFMYTDFIRHGTIFPYGGLFTDDYYHFSVVAMT
                     DVTYFYFPVDLFEEYSLENRLQMKHLYSKMSKLLELHELRVRNLITSSASSRVIQSLA
                     ILLVEMGKDSDTLPFQLTTTDIAQISGTTRETVSHVLRDLKKQELITIKGKYLTYLDK
                     DYFLQ"
BASE COUNT        14925 a         9237 c        10831 g        14701 t
ORIGIN      
        1 aagtgcaatt ccgcttcgcc tgaaaaatat tttttattta tcacttagta tggttttgtt
       61 tcaagtagtt ttttgggctt tctttccaga ccatttcatc cttgatgttg tgatgttggc
      121 tcagtttctt ttctttgcgc ttatagccct atactatggt aaatcaataa aagctaagtt
      181 tttaatgttt tatgcctttt ttccactagt gtctattagt ttggttaagc gatttattgt
      241 attttttgtt atgccattgt ttggtatgcc ttattcagtt gttaaacata atacactact
      301 gatttacagc attacttgct ttagtatttt tttgatttat cgctgcattc aagtttttca
      361 tttcgatttt tcaacctggc gccaatattt tcaatcacat agagctagta aactattagt
      421 gtttactaac tcttcgatgg ctctatatta cttgtgtgtc caagggatag atgtgatgtc
      481 gccttcttta tcaggacttg ctacaacgac tgctcgttca atcatagtgc tcttttattt
      541 cattttgttt cttactttat taattcattt agagcgttat gtaaaacaaa actctattga
      601 ggcaattgtg caacaaaaag aatatcgtga gctgattaat tatagtcagc accttggatt
      661 gctgtatcaa gatattcaag agcttaggca gctattaact accgtgtcca gtcgtcttaa
      721 gattggaatc gaacaaaatg atatctctat tgtgagactt acttatgaag gtatcttgaa
      781 tgctgaaaag aataatgcta aagatgacag acttgattta acttgtttag ataaattaca
      841 agttgaagca atcagacata ttgttttagc taaattaatt gaggcaaaaa ataagaagct
      901 taaggttgag gtatcgatcc ctaattgtat tgcaacgttt tttctagaag tagtagattt
      961 cactaagcta ttgtcatttt tgttagataa tgctatagaa atgagtttgg agacaaagca
     1021 accttgctta tcaatagcat ttctggatca aaaccataaa cttgtcatag tcattcaaag
     1081 cagtactaaa caaggacaag atgatagtca aagcgtgttt gctataccgg ctttgaaaaa
     1141 aagagatgac tggcaatttg acttaaggaa tgtcacgacc attttaaatc gttatgacta
     1201 tctcacaatt agctcgcaga ttcatgatgg cattttaacc cagttaatag aaatagctaa
     1261 gcctgactga tgttaaaggt atgaataatc actcaaaatc aacttaagag tctgagacaa
     1321 tagtcccaga ctctttttga atatgttaat ctattactaa tgataatagt ttattgtttc
     1381 ttccaagcta gagttaaaat ttccgctttg ccaagttggc tgggtgtgct ttgcttgctg
     1441 tctttttcta acaaattagt gaccttggca ttgtagtttg gcaggcttag gctaaagtca
     1501 caagttttat cgtttgagag attatagctg cgcgtgataa gggcattgtc tgctaagcga
     1561 cgtttgaaag ctgtgagggc aacttggtcg tttgtacccg tcaaatagct atattctgcg
     1621 gctaatgttc cctcgtgttg gtttgtttgt gtggtaataa cagggacttg gccttcttga
     1681 gctcgccagt agctggcaaa ttgtgtttgc ttagtgatgc tttcaaaact ataagaaagg
     1741 ctgtgcttgc cgaggcattg ggcttcagga gtagggaaat acccccagtc gcccatttcg
     1801 ccaacagaac gtaagagagt gatggcaatg gtgttggtat ctggtaagat ctcatattcg
     1861 ttgaggccat agttaccaat agtgactcca ttttcaccat caaagagact cacaaagcat
     1921 tcttggtgct gtgggttact tggattcttc caaaaggtgg catctggatg atttggacgt
     1981 ttgacagttt caaaaatact gtcagctaga tgatggtctg ttttaaggtg cgttgggaat
     2041 aggacgcgca agcgatgatt tgtcatttgg ttatcaaaac gtgtggtaaa ttggaggcga
     2101 ggattgtttt tctccatacg gataagggtt gttaaggtta gctcagcctt ttcttgtgaa
     2161 cgtcttgctt ggcgttctgt gatgtcaatg acagcttcca tctcagcctg taagcggtca
     2221 tcggcggaga caggaatggt aaaggtttgc tggatttcaa gttcagcaac ttgaatagtg
     2281 ttgctgcgaa cagaaatatg agttacgcct tggtccgcat agaaaggttg gtcatgagtt
     2341 ggttggcgag aaatatattc atttccaata tcgccacaat cttcaaaccg caacaggtct
     2401 tgatagatta gtccagattg tttatcggtg atggttagac ggccatcggt ttgtatcata
     2461 acttttagaa acccattttc aaggcactga ttactgtcat cgtagaggga atcggtttct
     2521 gaaggagttg tttcatttcc tagctttaat gctaaggttt tccaagacat ggctgggaga
     2581 gttattggta gccgtaatct cactttgatg gcgaaataag gttcgcggaa ggatctctcg
     2641 ggcaaatcgt agtcaaaagc gatgcttgtg cctaaaattt ctgcttcggg actcacttgt
     2701 ccactagtgt caataatttg gaaagattgg gagagtcctg ccaaatattc ttgagcttct
     2761 tggtaaacct ctttaggaaa gcgttgacca aaatgatatg ttttccaggt caggctgagc
     2821 tcagcaacac ttgttttgga atgaccgctg gtattaaata agacgaaggg ttggctatcc
     2881 attggaaaat ccctggtatc aatggcgtca gcaatttgct tagcagcttc ttttgctaaa
     2941 tagtgtccga cttcataggc tttttcaaag cgcgtcatca tttcccgatg aacgctatca
     3001 acactacaac cacagataga atcatgaggg tgattttgca tgagggtttt ccaagcgtaa
     3061 cgcagttggt cgtgagggta ggtacttgtc acctcataag ccattgctgc taagggttcg
     3121 gtgatgtttt cgagttggcg agagactctg gtattagcct gtttgaggta aatacgagca
     3181 gaagccgtgt tagctagggt ataccagcca tcggtttctt gactggtaat ctctccttgg
     3241 acggttgaaa ggttctctgg taaatcatct gcgagatcag ccaagtaatc ttcaaagcag
     3301 gaatgcacaa attcgtagtc aggatagagt tggttggcta aggcgattgc cttggtgaca
     3361 tcaagttgta cgggttgatg atcacacccg ttcatcatca gaaggtgctt ggttgaggcg
     3421 aagcgttcgg cgtcagcaag ttttttatcc caaaaaagac gcgcctcagc ttctgttgtc
     3481 gggatctcat tgccattgct gtaccagttg gcgaagagga gtccaagaat acgactgtca
     3541 tctggtcctt gccaactgat ttcagagaat tgggagctgt atttttcact ggtatctact
     3601 tgattgttaa atccagttgg acgaatgcca cgaccaaagg cagcagcttg taggccggct
     3661 tttaacatca gctgtggtgt ttgtcccata tttccaaagg tatctggaaa ataaccaagt
     3721 ggcacactag cgccccattt gtcacaatcc tccttgccaa tgagcatatt gcgcacattg
     3781 gactcgctgc tggtcaaaaa atcgtcctgt aagatataga aaggtccgat acggagtttt
     3841 cccgaagcaa tggcttgtct aatctcaggt tctcgttcgg ggcgtacttt taaataatca
     3901 tctaggataa tggtttgacc atccaaatga aaactatgaa aattaggatt tgtttgaaaa
     3961 acttctaaca ggtcatctat taagttaatc agacgcatgt ggtgttgctc gtaagccatg
     4021 taccactcgc gatcccagtg actgtgtgaa ataatatgta cttttttggt tgccatagtg
     4081 gcctcacttt ctaaggagca atgctaaagc caagatagtc aagaagtagt tcacagaaca
     4141 tcatgttagc ccaagagaac cactcacggg tgtattgata aggatcattg acatcgaaac
     4201 cctcgtgcat taagtgggta ccagcatctg ttgccacaag caaatcaagc atctcttttt
     4261 tactgtcttg gtcaagagcg gtcaatcctt gtaaggcaag ggcgatatgc cagatataat
     4321 tttctggtgt atgggaagaa ccgatgcctg ctgctgcatt tccctgataa taataagggt
     4381 tttcttggct gaaaatggtg cggcgcgttg ccagatagat agggtcatct ttagcacaaa
     4441 aaccaaggta aggtgcagcg aggaggcttg gaatattagc atcatccatg atggaaaact
     4501 gtcccagacc atccacttca taagcgtaaa tggtttctcc cttgctgttt tgaacaaggg
     4561 catggtctgc aatacctgat tgaatagctt cttttagctg cttggcacga gtagctattg
     4621 gttcattgtg aaaaaggttg ttatataaat cttctagata ggaaagcaca accactgcaa
     4681 acatatttga aggaatgagg taaccatatt gacaagcatc atcacttggt ctaaagcctg
     4741 accaggtcat tcctgttggt gcgacaggac ttccttttcc agcatgtgtt aaggtatctt
     4801 ctttgcgcca agtatcgcgt tcaaacaagt aaggagactg ggcgtggtct tgctcaactg
     4861 tccataggtc aagaatgagt tctaccccac gatgaaaatc gtcattaaac tgatcggtgc
     4921 ttcctgtttc acgatagagc agataagcca gttgaatagg gtagcaaagg gaatcaatct
     4981 catatttgcg ttcccaaatc caagggttca tttgggtatg atctgtttgg tgtccttttt
     5041 cgttagcttc ttcgttgaag gcattggcat agggatcgat acaaatgtag cggaattggc
     5101 gtttgaccag accggcaata atctttctaa tttcttcatc ttccttggcg aggaagaggt
     5161 aaggttttat ttgcgcagtg gaatcacgta accacatggc aggaatatcg ccagttaaaa
     5221 gaaagctagt gccatcttcg taggttttta gggttgtctc taaggtattg gtaaagctgg
     5281 ttttgaaatt aagagcccaa tcagggtgtt ggtctccaca aaggctttcc accttggcca
     5341 taaattggct gacgctagtt gggattggtc ttgtcatgtc atatcctcca aaatgatata
     5401 tcatttatct tcggtttcat tataaatgat aagttaaact ttgtctataa gtttttgaaa
     5461 aggtttacaa ctcttttttg acatgttata ctgataacag gagtaaagaa tgacaaaatt
     5521 attatacaaa aagatatatc atttcattaa agagcagata gcatcaggtc gtttgcaaat
     5581 tggtgaccgc ctacctactg aaaaagaatt gtcagaacag ttctcggtta gccgtattac
     5641 ttctaagcga gcgcttgtag aactggagca agaaggcttg attacgagaa gtcgtgggaa
     5701 aggaagcttc gtagctaaaa agcaagtgac gtcccctgaa gcgaataagg atttattgtt
     5761 gattcttcct tttgcctcag attatgaatt gggggattat gctaagggaa ttatgacctc
     5821 cattgctgaa acagggtatc ggttgatggt gcaactggcc agtacggtaa ggttagacac
     5881 cctgtcagac tatgctggga ttatttatta tcctgaagat gtcaatcaca gtattgattt
     5941 cctgttttac tgcgaccatc atcacattcc gctagtcttg ttggataaat ctctggacct
     6001 ttttcagttt ccggctgttg tggcggataa taaaggcggt gcctatcaat tgactcagca
     6061 cttgattgag caaggctgcg accagatttg gtttgtggca acaggctctt ttggagaagt
     6121 atcatccgtt agagaccgtt atttgggtta cttagcggca atggcagaat cgtctctaaa
     6181 atcccattat tttcctaaga cagagactga aacaacagat gcttatttgg accgtttggt
     6241 gaaaacctta tcggagacga cagccactaa aagggggctt gtcgtggaaa acgattggtt
     6301 ggctattcag ctgattcaaa aaatcacaca ggtaggctta tctattcctg atcaagtcgc
     6361 tattgtaggc tttgataata gtcaagcctc ccgtttgttg agtcccaaat taagcacagc
     6421 agctcaagat ctttaccaaa tggggcaaga agcagctaaa gtcttgttgc agattattca
     6481 tgagccagac caggcagtgg tgtcttgcca gttacctgtt caagtattta tcagagaaag
     6541 tagtcactaa aaaagatgtt actagcatga gctggttagc tcaggtaaag gagaccagaa
     6601 atgaccattt atcacgcctt aaaggattat caagaagtca tgacaagagg agactatctt
     6661 gtctttgaca cgccattgac ttgccgattt attggccatt ttttccgttt tgaaaatcaa
     6721 aaagccctgc tggcagaatt ggcaacgtca aagtattttc agtggattga agaaggccaa
     6781 gcagaggtaa cgatgaaaca cttttttaac cgtcagctgg ctaaggatgc ctttaccctg
     6841 aaggtttcag aagataagga aatcattatt gaaagccaaa accttagagg ttttcgttac
     6901 gcccaagaag ctttgcttaa agtcatgacc ttcaaagggg ataaacttta tttgcccatt
     6961 gtttctgtca agcacagccc gtcatttcct atgcggggaa taattgaagg cttttatgga
     7021 acaccgtgga cacgagagga acgaatcgat tgcctacgtt ttattggaaa taaaaggatg
     7081 aatacttaca tgtatgctcc gaaagatgat gattaccagc gaaaactgtg gcgcgatctt
     7141 tatcctgagg attgggtgac ttatttcaaa gagctacttg cagtagccaa ggaagaaggg
     7201 attgattttt ggtatatgat tagtccggga cttgattttg attataccaa ggaagctgac
     7261 tatcagttgc tctatcaaaa attgcaacaa cttctagcgt taggtgtctg tcattttggg
     7321 cttttgctgg atgatattga ctaccaaatt gtagatgctg ttgagagacg attcaaaaag
     7381 actgcctatg cccaggccca cttggcaact gaggttcatc actttttaaa ccaacaacat
     7441 gcggcgccag aattggtgat ctgtccgaca gaatacgaca accaccatga ttctatctat
     7501 ttgcaagagc tgtcagaaag gattcctaaa gaagtcgcct ttttctggac aggcccaagc
     7561 acgctagcga gtcaaatatc gcaagcagat attgaaacta tggcagcagt atatcaacgg
     7621 cctatcatta tttgggacaa tattcctgtt aatgattatc aaaaagatcc agaacgtctc
     7681 tttttaaccc cttttgctaa tcgctcgcca tttttatgcc agccagacta tcaggtcaag
     7741 gggattgttt ctaaccctat gattagttgg gaattatcta agctgacctt gacagatatg
     7801 agccattatc tgtgggatgc taaccgttat cagcctagtc attcttggct agaaacctta
     7861 acagactata cagaagatac tgaattagcc ctagctttac aggcatttgc ttggcataat
     7921 ggcaatcgtc atctgcacag ggaccttcct tttgaagtgg aagaagctct cctggctaag
     7981 gatgtatcaa cgttatcagc ttgggtagct gaattggtcg aatgtgtcaa tacgttgaga
     8041 aagctagata agccagcgtt tcaacaagct attgctcctt ggtttgaacg ggtggcaaaa
     8101 gatcaggtgt tttggcaggc tatgttaaat caggagcctc agttggaaac actctatgct
     8161 gacttacaag aagataaaca ccgcattgga agtgacattc caagtcgtta ctatcgcatc
     8221 tactatcaac agcaagataa actaacagct aatcaaggtc aagttaccca agcaagacct
     8281 gaagattatg cttagtcata cgagctagaa aggaatgata tgttagcatt tccaaaagag
     8341 ttttggtggg gaggagccac ttcaggccct caaagtgagg ggcgttttgc taagcagcac
     8401 cgcaatctct ttgattattg gtatgaagag gagcctgact tgttttacga ttacgtggga
     8461 cctgatacgg cttcggatgc ctatcatcag attgaatctg atttgacctt gttagcttca
     8521 ctaggtcaca attcctaccg cacctccatt cagtggacac gtttgattga tgattttgag
     8581 caagcgacga ttaatcctga tggccttgct tattataatc gagtgattga tgcttgcttg
     8641 gccaatggta tccgtcctgt tattaatttg catcattttg acctgccaat tgccctctat
     8701 caagcctatg gtggctggga atcaaagcat gttgtggact tatttgtggc ctttagtaag
     8761 gtttgttttc aagaattcgg tgatcgggtt aaggattggt ttgtccacaa tgaacctatg
     8821 gtcgttgtgg aaggctctta tttaatgcaa tttcattacc ctgctatcgt ggatgggaaa
     8881 aaagcagtcc aggtcgccta taacctagcc ctagccacag ccaaggttat tcaagcttac
     8941 aggcaaggtc cagcagaact tagtgatggg cgtattggaa ccattctcaa tctgacgcca
     9001 gcctacccag ctagtcaatc agaagcggac atggctgctg cgcattttgc agaactttgg
     9061 aacaatgact tgtttatgga agctgctgtt catgggaaat tcccagaaga attggttgcg
     9121 gtattgaaaa aagatggggt tttgtggcaa tcaacgccag aagaattggc tcttattgct
     9181 gagaatagag tggattattt gggattgaat ttttaccacc ctaagcgcgt gaaggctccg
     9241 gatgtcattc cagtgattag cccatcttgg agtcctgaat ggtattatga tccctacctg
     9301 atgcctggcc gccgcatgaa cgtggataag gggtgggaga tctatcctga ggctgtctat
     9361 gatattgcca tcaagatgcg agaccattac gacaatattc cttggttctt atctgaaaat
     9421 ggtgtaggga tttcaggaga agagcgttac cgtgatgaaa ctggtcagat tcaagatgat
     9481 tatcgcatcc aatttttgaa agaacatttg acctacctcc acaaaggaat agaaacaggc
     9541 tcgaattgtt ttggatacca tgtatggacg ccaattgatg gctggtcttg gctcaatgcc
     9601 tacaaaaacc gctatggcct tgtggaaaat aatatccata cccaagtgcg acgacctaaa
     9661 gcttcagctt attggtttaa aaaagtagct actcataacc gcttgatttc attagaagta
     9721 atggaggagt ttggaggctc tgcttctcac ctcagtgatt gaacgtgtga ccgtctcctt
     9781 tgagacaagt gaataatgat gttaccaatg attaaacaag ggcctttttc ctcatgttaa
     9841 gaggatcgag gctcttgtat ttctttggaa agtgttggta tttgccgaac tagatataag
     9901 cgttctgtct ggtttggcat catctatcca ccttgggtaa tttggctctt caattcttta
     9961 gagtatcttg gctaaggatg cttgtttttc atcttggtat tttaggattt tctttcatat
    10021 ttttggacca gataaaagcc cctcatggtt tctagtgact gtttgttatg tcactgtcca
    10081 cgataagggg actatatgag tgagttatta agtgaggctt tgcttattgg cgataactag
    10141 catagcttct aatagctcga gcattaactg atgaccaatg cctttaacag aagcgtcagc
    10201 aacaacgaca tcaacaaagg gaccaggcat caggctgcgt tcatcagcag agagtcattt
    10261 aggcagaaag ccagtatcgt tgtagctaat cagcaatcct tttaggatgt ctttgtagat
    10321 gtcagaggcg attagggaca ataggggata agagggattt agcggtccat ggttaggcct
    10381 ccttgtgttc ttggtaaaaa tcaatggcag ctccaagaag attggcttcg tttttaaagt
    10441 gacaaatagc taattggggt ctaaggtcgg agatttcgac ggcttggtag agtttgtcta
    10501 gttcagcttc aatcgctgac agtagaaagt cagcttggga aacaccaccc ccgattaaaa
    10561 tcagttgagg atcaaaggcg tgttgaatgt taaaaatacc gatggctaaa ctttggagaa
    10621 agacctgcct ttctttgatt gctaggtggt ctccttgttc tgcgagggca aggacagctt
    10681 tgccagtata atcttttccg ttattgacga ttgcactata gcgcttggcc atatttacca
    10741 ctgtccctaa ctgactaaag gtttggtagc ggtcgttcat gatcatgaaa ccaaattcgc
    10801 ccccaaataa gtgggcacca tggtaaatct tgccatcaat gaccaagctt cctccaactc
    10861 cagtaccaag aacgagcatg gctaggctac tagcgccttt tcctgcccct aaggtagctt
    10921 cagctaaggc ggcacaattg gcatcatttt cgatagaaat agggtaatgt agccgttctt
    10981 ctaagacctc ttgtatttta aaatggtgaa tgtagggaat agcgctggca ccttcgataa
    11041 tacctttttc cttgttaacc gctccaggag cactaacggc aatacctgag aaatggtaag
    11101 cagagtaacg agcaacttct tggtcgagta attggtaaaa cttttccaga ctagagggag
    11161 tgggaaagct gctttgctgg cttaattggc cattgtgaca gagggcaaat ttaagggaag
    11221 tgcctcctat atcaatacat aacaagctca tgaggtgttc tcctttatta tagtagaata
    11281 gacgatcatg tctcatctta gtatgacaga tttaaacaac aatgaaaagg ctttctaagt
    11341 gattctatac tttgtgttat catacctata atttgttgct ttcaaataat gaaagcgctt
    11401 gatatactgt aactataacg tgattaacac aaagggaggt tgggaaatga caagacaaac
    11461 caacaaaaag acatcctttt ggaaaaatgt gattaaatac cgggccttat tattaatggt
    11521 attaccaggt ttcatctggt tcatcttttt cttttatatt ccagttttgg ccaatgtggt
    11581 tgcttttaaa gattttcatt attcagcagg gggctttatg gaaagtttga aagagagccc
    11641 ttgggttggt ttggctaact tcaaatacct ctttgcttcc aaagatgctt ggttgattac
    11701 cagaaatacc atcgcttaca atgtgatttt cttactcttt aatgttttct ttgcgattgc
    11761 ttttgccatt atcatgagtg agttacgaaa taagcgaacg gttaaggtct atcacaccat
    11821 gtccttattg ccttatttct tatcatgggt ggttattgaa tactttgttt ctgccttttt
    11881 aaatacggat aaaggtttca ttaatcagct cctgactgga agtggagcag atccaatcaa
    11941 gtggtattcc aatcccactt ggtggccctt gattttactc tttatgagtg tctggaaagg
    12001 gctaggctac aatagcatta tctactatgc ttcagtcaaa gggatttctg atacttatta
    12061 tgaagctgct atggtggatg gtgcaagcaa atggcaacaa attaggaaca tcaccattcc
    12121 acaattattg ccaatgatgt ctatcctatt gatcattaat attggtaata tctttaaatc
    12181 tgatttcgga cttttctacg ttattcccaa aaattcaggc ccactttatg acgtgaccag
    12241 tgtgttagat acctatgtct acaatgcctt gactgctaca ggagatattg ggatggcatc
    12301 ggcagctagc ctttatcagt cagtagttgg gaccagcatc ttgttagtca ccaatgcgat
    12361 tgttcgtcgc atggaccctg atgcagcctt gttttaggag gaaaacatgg agaaaaagaa
    12421 aaaagtcgaa aaggttaatg ttagaacctt tgatcgaaaa accaatgcta tctttaatat
    12481 tctgattggt ctgtttgcca tctcttgtat cattcctttt atcttcgtga ttattatttc
    12541 cttcactgat gaaagttact tgattaatca tgggtatagt ttttttccag atgtctggtc
    12601 aactaaggct taccagtata tttttcaagg agccatgtcc catagaatca tgaggtcatt
    12661 tgggatatcc gtgtttatta cggtggtggg aacctttatt aacacgacca tgacatcaac
    12721 ctatgcttat gcgatttcaa gaccttattt cccatacaga cgttttttta ctgtttatgc
    12781 acttatcacc atgctctttg caccagggat ggttgctaat tacttggtgg tcagcaatct
    12841 ccttcatttg aaggatacgg tttgggcttt gattttacca atggctctgg gcccatttgg
    12901 catcttggtc atgagaacgt tctttaaaaa gacagttcca gatagcatta ttgagtcggc
    12961 tcgtatggat ggggctagtg aatggatgat ttttatgaaa attgttctcc ctttggctgt
    13021 tccagggatt gctaccatca gtttattttc tgccttaact tattggaatg attggtttaa
    13081 cgccttgctt tatgtgcaaa gtgaaaacct ctacccaatg cagtacttac tgatgaaaat
    13141 tcagagcaac ctacaggcct tggcacaaaa tgctggcatg agtgcacaaa tggcagatag
    13201 cttagcatcg ctgccaaaag aatcagttcg tatggctatc gtggttattg caaccttgcc
    13261 gattgccttg acttacccat tcttccaaaa gtactttgtc ggtggtttga ccattggtgg
    13321 ggtgaaggaa taggacttaa ggaactggtc acaagaccag attttatcta atcattagta
    13381 acaaaagaaa acatttttta ggaggcttca cttatgaaaa agtggcaaaa aatcgtgtgt
    13441 gtgactggaa ctgtgcttgc agcgtctagt ttagcagcct gcgaaagcaa gtcagcatca
    13501 aaggatagtg atgtcaaatt attgatgtac caagttggtg acaaacctga taacttcgat
    13561 gaattgatga caattgctaa caagcgcatc aaagaaaaaa caggtgcaac ggttgacctt
    13621 caatacatcg gttgggggga ctgggatgat aaaatgagca ccatcattgc ctctggtgaa
    13681 aactacgaca ttgcttttgc caataattat gtggtcaatg cacaaaaagg tgcttttgct
    13741 gatttgacaa cgttaatgcc aaaatacgct aagaaaacgt ataaaaactt agacccagcc
    13801 tatattaaag gaaatactat tgacggtaaa ctctatgcct tcccagtaga tgccaacgtt
    13861 tatgcccaac agatgctttc tttcaataaa gaactagtgg acaaatatgg ccttgacatt
    13921 tcaaacatta agtcctatgc agatgctgaa aatgtcttga aacaattcca cgaaaaagaa
    13981 ccaaatacag cagcttttgc tattggtcaa gtctttagta tgtcaggtga ctatgactac
    14041 ccattaacca aaaaccaacc ctttgctgtg aaaattgatg aaggtaagcc aaccatcatt
    14101 aatcagtatg aagatgagtc ctttaaaaac aatctccgct tgatgcacaa atggtataaa
    14161 gaaggtttga ttccaacaga tgcagcgacc aatacagaag gttatcccct tgaaggaaac
    14221 acttggttta tgcgtgaaga aacccaaggt cctatggact atggcgatac tatcttgacc
    14281 aatgctgcag gaaaagacat cgtgtctcgt ccattgacta aaccgctaaa aaccacatca
    14341 caagcacaaa tggcaaactt tgtggtatca agcgtatcta aaaacaaaga aaaagcagtt
    14401 gaagtccttt ctcttcttaa tagcgatccc gaattgttaa atgggcttgt ttatggtgtc
    14461 gaaggcaaag cttgggaaaa aattggcgac aagaaaatca aactgctcga tggctatcaa
    14521 ccaaaaatgc acatgggtgc ttggaatact ggtaacaata aaatccttta cactcaagaa
    14581 tctgttactg acgacatgat tgctaaacgt gaccaatcaa tcaaggatgc taaagaatct
    14641 ccaatccttg gtttcactgt tgacacaaaa gttatcaaaa cggaattgtc aaatatttca
    14701 aatgtcatga accgatataa agcaagtatc aatactggta cagtggatcc tgacgaagcc
    14761 cttccaaaac ttcttgcaga cctcaaaggt gctggttggg acaaggtgca aaaagaagtt
    14821 caaaaacaat tggacgactt tgtagctaaa gacaagtaag gcattcattt tcttgattat
    14881 aagttgaggg ctgggaaatc tcagctcttt tcttctgatg agtggaaaaa atggatcatc
    14941 aaataagtgg aagttataaa gggagaaaaa ttaatgtaaa taatgttact actcttagta
    15001 aagcggtttc caaaagccta taaagtgtgc tatattagaa gagtctaaac tattaatcag
    15061 agtaaaacta ataccagcat cttattgggg gtgctaaaac tagtctcgta gctgctggtc
    15121 cctgaaaccg ctataagtgt taacaagaaa aagaggacta tcatatgaat ggcattgaaa
    15181 aaacctgctt tttaatttgg aaaatgatcc aattgaccct catttttcat ctcttgtcct
    15241 tagctggact gattgtattt ggggtggggc cagcctggca aaccattgtc accctatttt
    15301 taaaaacatc tcaagaagaa aaccactatt ctttgaaaag tgctgttcaa ctttggaaat
    15361 cttatttcaa agaagctaat ctaagatttg gtctttttgc cctgactttc ctttttttaa
    15421 cctttaattt gcattgggca gtacagtttc catccttgtt ttggtttacc gtgtctttct
    15481 tgattgtaat agccatggtt tggctaacca tgacctatct ttacatggtt ttttatgcag
    15541 tgagctatga gattagcctt tggaataata tgaaattagc ctttatcagc gtctttttaa
    15601 gtttcaaatc ctttttactc atgctgagcg ttttgctggg aattatctta gtgacttggc
    15661 aatacaaagg tctttatctc tttttaacct tcggagctct agtcttttgc ttggactttg
    15721 tcactaaggc taatcgtcga ctagtagatg gagtgattga tgagtgttaa aacattggga
    15781 aggtatgtga ctaaccactt taagcagggt tttttgatta atcgactgat gaaattatat
    15841 agcctgctaa ttgtaagttt tttcacttta gcagctattg gcttgagcag ttattctatt
    15901 agtagtactt ataaaagggt ggatgctgag gcgcagatgc gactggagga gactctaggt
    15961 agattgcagt cacaaaacga tattacccta cgggttttag accagttggt tggccgttca
    16021 gaagattacc ataacctcta tcaatatatg acgttaaccc caaaccaata tttttcaaac
    16081 gtttttgaag actgggaaaa aggaaaagac acggtcctat tttctgaaga ggtgaggcgt
    16141 ctttttgact tgtaccctga tgtgacttct atgaccctac tgctagagga cagtagtgct
    16201 tatctgtacg ccgatagggt cattaagacg ggtcgtttgc tttatagcaa gcctgaaaaa
    16261 gtaaccggaa atgttttagt tcgctccatt cgaaatccag aatcaggaga tgtgacaggt
    16321 cgtctttacc tgacctttga caaaccaccg actttggtag agacacagca ggatcactat
    16381 ttagctactt ttgcttttga ttactatggc cgcaaactct tccatcaagg aggtcggaga
    16441 tttcctcatt tagaggctga ggttaaaaag gctatcaagg cagatcgggc agctgatctt
    16501 agtaatctca gcaaggctta tcggatgcag tacaatcgct cgggagacct cttagcttat
    16561 gtggctgtca gaaaatccta tttattagcg gaagctgtca ggacagtttt tgtgcatggc
    16621 cttgtttctc tactcttagc ttggctgtta ttgcagttgc tctttagggt tttccgaaat
    16681 tatatccagc aggtttctga aattacggat actgttgaaa tggttgcagc aggcgatttg
    16741 tctttaacca tcgacaacag ccacatggaa ttggaactgt atcatatctc agaagccatt
    16801 aatcagatgt tggccagcat taaagcttat attgacgaag tttatgtgtt agaggtagag
    16861 caacgagatg cccaaatgag agctctgcag tctcaaatca accctcattt tttatacaac
    16921 acgttagagt atattcggat gtacgccctt agttgtcaac aagaagaatt agcagatgtc
    16981 atttatgcct ttgcgagtct gcttcgcaac aatattagcc aagataagat gaccacctta
    17041 aaagaagaac tggctttttg tgaaaaatac atttacctct accaaatgcg gtatccagat
    17101 agcttcgctt atcatgtaaa gattgatgag agtattgctg acttagccat tcctaaattt
    17161 gtcattcaac ctctcgttga aaattatttt gtacatggga ttgattatag tcgcaatgac
    17221 aatgcactaa gcatcaaggc attagatgag acggatcatc tcttgattca ggtgcttgat
    17281 aatggacgtg gtattagtca agagcgctta gcagatatgg aaaaaaggct tcaagagcac
    17341 caaacaacag gcaatagctc tattggtttg caaaatgttt acctccgtct tttccatcat
    17401 tttcgagaca gggtttcttg gtccatggct aaggagccaa atggtggctt tattattcaa
    17461 attaggatta gaaaggatgc ttgatgtata aggtactatt agttgacgat gaatacatga
    17521 ttttacaagg gctcaccatg attattgatt ggcaggcact tggatttgaa gtggttcaaa
    17581 cagcaagatc aggcaaggaa gccttagcct atttgactca gtacccagtg gatgtcatga
    17641 tttcagatgt taccatgcct ggaatgacag gactggattt aatagaagca gctaaaacct
    17701 atcatcctca gctacaaacc ttgattttat ctggttatca agaattctcc tatgtgcaaa
    17761 aggctatgga attagaaacc aaggggtatt tgctaaaacc cgtggataag gcagaattac
    17821 aggctaaaat gaaacaattt aaagactgct tggatgccca gcaggcagaa tcaatccggc
    17881 aagaagccta tcatgacagc cttttgaccc tttggcttac agatgaattg agtgagaaag
    17941 aatttcagca gttaagtcaa ggcttgccag cggctgcttt aattggtttt acagtgctgt
    18001 atttggactg tcaagagtgg caagcagcca tcgatgctta ttttgaacaa gaaggtcagc
    18061 ctttttacct caaaaaagaa gaagataaga aggtttatct ggctgtccta cttggtaaaa
    18121 ctgaccaagc caaggcattt acctatgacc tacaaaaacg attctcaaga aacatgcatc
    18181 aacttatttt gggagaaaca gttgatgact gggaaaatgt ttatgaaagc tacaatcagg
    18241 tgcttaagag cctcttttac aattgtgaag tgtcaccaat tagagcaaaa gataagtcca
    18301 aaattgaact accagagtcc cgtttgcagt tctttgcttt taacaaggcg ttgatgattg
    18361 gggacgaacc tacaatcttg tctaaattag aagctatttt tgacgagatg aagaccttga
    18421 acttttcacc agaggatgtg aagcatgttt cgttcttgtt gttttctgac atttaccgtc
    18481 aatttcccat tttagataaa atgacttatc tgtccatggt aaaaactatt catgacagcc
    18541 aatccattga tcgcattttg agggaactaa aaaaagtgtt ggatgtcacc aatcagaaca
    18601 attcccctga aaaacgctat tcagatttag ttagtgaaac cattgattgc attcgcaagg
    18661 aataccatca agaattaacc cttaaagcta tcgctgatag gttacatgtt aatggggttt
    18721 atttagggca atgctttaaa aatgaaaccg agcgtagttt tacccagtac ttaaatcatg
    18781 ttaggattca aaaagcgcaa cagttattgc tttacaccaa ccaatccatc aatgaaattg
    18841 cttatgaaac aggttacaat accaatcatt attttatcaa gatgtttaaa aagttgaatg
    18901 gtttatcgcc aaaagagttc agagatcgtt acaaggacaa ttaccaagcc atcaagggcg
    18961 accagtaaca ttaaacagga gattgatatg acaccagata gcaaggagca gacagtgact
    19021 ggctaccaat accactatat tgatcaagaa ggaagaaagc agccctttaa tcaaggctgg
    19081 cgttttctta tggcagatgt ggcgtgtgct caggcccctt cttttgatga ttcgaactgg
    19141 caggtgattc atttaccaca tgatttcagt cttacgcagc cttacacaag aaatggtgaa
    19201 gctgaaagtg cttataaatt aggtggtgtc ggttggtatc ggcattatct cgtgttagat
    19261 gaggttttag ctggttgtca tgttgccatt acttttgagg gttcctatat ggaaacggaa
    19321 atttatgtta atgggcaatt cattggcaaa cacttaaatg gctatcagga atttacctat
    19381 gatatctctg atgtagtgac ttttggagct gaaaatcttc tggctgtccg ggttgaaaac
    19441 aaggttccca gttctcgttg gtattcaggc agtggtcttt atcgtgaagt gagtttgtct
    19501 gttcttcccc agcttcattt tgtggcagac caggtagcaa tgaccttggc tgatactgct
    19561 gtgcaggaga aagggcaaca gaaagttgat ttacgctttg cccttaatca gcctattcag
    19621 acttgccact accaattgag cctctgttta tgggaacaat cacattgctc aaaagacaaa
    19681 aaactccttt atcaagaaac agaagtgcct ctagctgatt tggctttcca aagacagtat
    19741 ggtcttacac tgtctttgga agaactccaa ttgtggtcac cagataatcc tcacctctac
    19801 gatttagaac tcactcttta ctatcaaggt caggtcattg attgcttttg ccttgaaact
    19861 ggttttcgtc aactgacttt tacggccaat caggggcttt ttgtgaatgg cagagctgtc
    19921 aaattaaagg gagtttgttt gcatcatgac caaggaggcc taggagcttg tgcttacgaa
    19981 gatgccctag ccagacaatt agtcctgttg aaagacatgg gggctaatac cattcgtagc
    20041 acccataacc caagtagtcc caaacttagg caactagcca atcgtctcgg cttttttgtg
    20101 attgaagaag cctttgacac ctggacctat gccaaaaatg gaaacgttaa tgacttttca
    20161 aactatttcc atcagactat cggaactgaa aatgcaaact atcttcaaag agtgaggtca
    20221 cctgaaacga gctgggccca gtatagtatt gaggccatgg tctggtctgc caagaatgac
    20281 ccgtcagtgt tgatgtggtc gattggtaat gaattgatgg aaggtttttc agccgatgtc
    20341 agtcattacc cagagctgac aagacaaatg tgccaatgga ttgctgctat tgacacctca
    20401 cgtcccatta cctttgggga caataaatta aaagaagctg acttttgctg gcatgaggaa
    20461 gtgtcgcaaa tggcaactct tcttagtcaa ttagaccatc cccagggact cattgggtta
    20521 aactatgcta atggaaaaga ctatgataag cttcatgagg agcactctga ttggctgttg
    20581 tatggttctg aaacagtttc agccattacc agcagggctt attataaaga gacaaaaaaa
    20641 gttcttgact ccggttatca cctaacctct tatgatcatg ctaaggtcga ttggggtgct
    20701 ttcgctagcc aggcttggta tgataccatt acccgtgatt ttgttgctgg cgaatgcgtt
    20761 tggacaggct ttgattattt aggagaacct acaccctgga acaagatcga cagcggtgtt
    20821 gtaggccttt ggccttctcc taaaaatgcc tactttggca ttttagacac ggcaggcttt
    20881 ccaaaagata gctattattt ttaccaaagt cagtgggtgc aggggcagac caccttgcac
    20941 ctgttaccag tctggcagaa ggaccagctt tgtttcgatg agcagggatt ggtggaggtt
    21001 gtggtttata gcaatgctgc cagtgtgcaa ctgatgtttg aagatgagca gggcaactta
    21061 acagattatg gaaggaaggc atttcatact tatagcacgc caacgggaca cacttatcag
    21121 ctttaccaag gagctgatgc tgctaagaat cctcacgaga atctttacct gacttggcga
    21181 gtgccctatc aaaaagggtt gctaagggca gtggcctacg acatttctgg taaaagcatt
    21241 ccaaaaacca gtggacgttc tcaggttaga acctatggtt cagtggcaaa gctatcttgg
    21301 aaagcctttg aagcacctat tgacgctcct tgggaattgc tttatctgga tttatcactt
    21361 ttggatagcc aaggtgaatt ggtcagccat gctcaagatt ggctaaaggt tcaagtagaa
    21421 ggaccagccc gtttgttggc tcttgataat gggaatccga ctgaccacac cccttatcag
    21481 gagcctctac gccaggcata tggcggaaaa ttattagcca ttttagccct cactggagag
    21541 gcgggacaca ttaaggtcac ggcaaaagcc tcgcagcaac ttagcagtgt ttttcagaca
    21601 caagtagcct gccgtgcccc caaagaacac ttagtgacac tacctttaga gaaatatcag
    21661 tttaaaaaga gccgactgac aagtcaacaa gaaattggtc aatcttgtct aaagaccaca
    21721 caattccttt ctgttttgga gaacaaagac acccagcaaa aagcaggtaa ggctagcaca
    21781 cctcacattc ttatcactag ttaccaacac tatttatctc tgcccttgca ggtttgttct
    21841 caggcatttg attttaaaaa aggcgatgat atcttcttgc ctcagtggct ccaagtcaaa
    21901 gatgcccaag gttcttttgt gaaaaaagct tttccaatga catggtgcct aaaagagaaa
    21961 atctttgcca agcctattct tgtcacgggc tatgtttctt gttttgggca atcaataccg
    22021 gttgaggcaa ggcttaacct agcacaagct agcaaaagac ggcaccagga tctcagttta
    22081 gtcgccaagc aatccctggc cacaagtcaa aaaaggctaa cttattgcta ccaatatgat
    22141 acggtgcaag ccataggagc cattagcttg caaaatcaca caggcacatc tcttagtgtt
    22201 tctgtggcaa tcggttatgg tagtcagagc tctattaagc aagaaacctt gatgctagct
    22261 gatagccact tgagccagtt agaagaggtt attttcttcg atgatatttt gactgttttg
    22321 tatctggcag tgatccttga agtcattgaa ggacctgacc taactgaaga cagtattcaa
    22381 attggccttt gggatttagc atgaagcatg agtaaaagaa gacttctttg gcaccaggtc
    22441 tgatgaattc tgtaaaataa ggtataataa aaaacaaata ggcgtccttt attacgtgaa
    22501 aggcgccttt acaaggttga aatggaaagt gagatttcta aatgtcagaa cgtttatcag
    22561 gccacaccct gttggtctct ttgcttgcta cccctattcg tcatagtttg tctcccaaaa
    22621 tgcacaatga agcctatgct aaactggggc tggattatgc ctaccttgct tttgaagtag
    22681 ggacagagca attagcagat gccgtgcaag ggattcgtgc cttaggaatt cgtggctcca
    22741 atgtgtctat gccaaataaa gaagctattt tacccttatt ggatgacttg tccccagctg
    22801 ctgagttggt cggtgcagtc aatacggttg tcaataagga tggtaaagga catctcgtgg
    22861 ggcatattac agacggcata ggagcgctca gagccttggc tgacgaagga gtgtcggtaa
    22921 agaacaaaat cattacccta gcaggagttg gtggggctgg gaaagctatc gctgtccagc
    22981 tcgcttttga cggcgccaaa gaggttcgtt tgtttaaccg acaagctaca cgtctaagta
    23041 gcgttcaaaa gcttgtgaca aaactgaacc agctaacaag gactaaagta acccttcaag
    23101 acttggaaga tcagactgcc tttaaggagg ccatcaggga atctcatcta tttattgatg
    23161 ctacaagtgt aggaatgaag cctttagaaa atctaagttt gattaccgac cccgagctca
    23221 ttcgcccaga cttggtggtc tttgacatcg tgtacagccc agcagaaacc aagttactag
    23281 cctttgcccg tcaacatggc gcccaaaaag tcattaatgg gctagggatg gtgctttatc
    23341 aaggagcaga agcttttaaa ttaatcactg gtcaagacat gccagttgac gctattaaac
    23401 ctcttttagg tgatgagtaa cactttttaa gtaagcttaa ttatgatata ttaataagag
    23461 atggagcagt catcatgggc tgctaaaatt ccttttgcga gagctgtttt taagagcaaa
    23521 aagcttattt gaagagcctt atccctaacg tgatacaagt catagttcct ttgaggtctg
    23581 ccagataacc taccgttttt ttagaaaaga agctcttata aaaggaaaag ttcaatgaat
    23641 aaaggagaat agcaatggta acaaaggaag ttgggcatga ctttttagca cgtctaggaa
    23701 aaaaacgctt aagacctggc ggtgttgagg cgacaaactg gttgattgaa caaggacaat
    23761 tctctcagga ttgtcacgtt ttagaggttg cctgcaatcg ctgcacgaca gcaatagagt
    23821 tagccaaaac cttcggttgt cagattgatg ccgttgactt aaaccccaag gttgtcaaag
    23881 aagcacaaga aagggttgct gttgaggggt taacccgtca cattacagta tcccaagcca
    23941 atgctttatc actaccattt ccagataacc attttgatat tgtgattaat gaagccatgt
    24001 taaccatgtt gaacgcccaa gccaaggaga aggcactcaa tgaatattgg cgcgtgttaa
    24061 aaccaggcgg gcgacttttg acacatgacg tagcttatga agatccaaaa acaagacaag
    24121 tacttgacga gttacgtcaa acgattcatg tcaatgttgc tcccttgtca ctaacaggtt
    24181 ggcaagcctt atatcaagaa cacggttttt ctcaagtgac ttatcaccat gggaaaatgt
    24241 ctttgatgac acctcgtgga atgattaagg atgaaggtct tatgaacacc ctgaacatta
    24301 tgagacgagg ccttaagaaa gagaacagac aacagttttt aaagatgcgt cgttttttta
    24361 ccagaacagg caaaaatctc tgctatattg ctgtaaaaag tgtaaaataa accttagacc
    24421 aacctgtcca tacaactaaa cagaccatag aggagttcga agatgtctta tatcaataat
    24481 attgagcacg cgaaggttct tgatttgacg caagaagtaa tgattgagca agatcaaatg
    24541 ttaagtagaa cactagtcca aaggcaagac ttaggaataa cagttttttc cttagataag
    24601 ggccaagaaa ttgggcgaca ctcatcacca ggagatgcca tggttactat cttaagtggc
    24661 ttagcagaaa tcaccattga tcaagagacc taccgggttg ctgaaggtca aaccattgtg
    24721 atgccagctg ggattcctca tgccttatat gcagtagaag cctttcaaat gcttttagta
    24781 gtagtaaaac cggaggttta acatgattga aaatctctct caactggtgc aatatcatga
    24841 tcatcaggtg gctagccgat ccttgagccg tctactggga ttatctcaat ctattgtttt
    24901 atatgccatg gcccaaggag aaagcattag tcaagagact agtccaaaag ataaagtaat
    24961 acttgtttta gaaggtcagc tcattttcga tttagaggat caaaaacagg tcctaactca
    25021 agaaagtctc atagccatac ctgcccaaaa agtccatcat ttagaagcta aaaccgattg
    25081 taaactatta caagtgcttt tagattaagg gcttattgtc aactgtattg ggtggcctat
    25141 ggaaaagcaa aagagaagat gacgctcatc ttctcttttt acgtattcaa agcgatgaca
    25201 atacgaattt taaggcttgg ctttctcatc tccagattat gccctttagc ttggtttagt
    25261 ttagaattca ctaaaaaaac cttaatgagt ttcctatctc cttataggtt tctcattaag
    25321 gctttttaga cgaaataatt gaacgacagg caaaagaaag atctttttac gagtgatacc
    25381 gttctcctat agtttcataa atcctctttt tgatgttgct gttagttatt cttagctaaa
    25441 agttatattg ttatcaagac ttgtcttgta gtaacatgtt ttaggagttc caggataccg
    25501 ttattctctt agcaagctta actagctaga gtggagtcac tatttataat aaactgtcga
    25561 gcttctcgac aaattggtga tcgtctttgt taatggctag ggtggtaaaa ccggtttctg
    25621 tgggtaatat aaagataata tgttgctcag aattcttctt atctgtttta aagacgtcaa
    25681 agagggatgt cgcagagaca tcagctcttt caagtgttgt tggtaactgg taacgttcaa
    25741 aaacttgaag gaggctatcc aagtcctctt ttggcaagag tcctttggaa acagccagtt
    25801 tagcatcaac caccataccg atagcaatgg cttcaccgtg atggtaaaaa tcatggtggc
    25861 agagcgattc aatcgcgtgg cccaaggtat gcccaaaatt taaagacatg cgcaaaccat
    25921 tttcaaattc atcttgttca acaatctgtt ttttattgga aatggattgg taaatcagac
    25981 gttcaagtcc ctgcttatcg gtttcttttt gaaaatcacg taactgctga aaaagcggtc
    26041 tatcatgaat gaagccgatt ttgagcatct cactgatgcc gcaggagaat tcccgttgcg
    26101 gtaaggtttc caaaaaggtg gttgaaataa tgatagcttc tggagggtaa atgctgccaa
    26161 tcatattggt tagactttta aaatggactc caactttgcc cccgatactg ctatccacct
    26221 gactaagtaa agtcgttggg atttgaatgt agggaatacc acggtaatag gttgcagcga
    26281 caaaaccacc cagatcccca acaacaccac caccaatagt aacaatcata tcttttttgg
    26341 aaaaattttc agcgatgagc tgatcgtaga tagcactgac acgttccaaa gatttggttt
    26401 gacctcccgg ggggcagaca tggacaaaag cgttgtattg ctgagcaaat tcttcaaaga
    26461 gagtctgata caagtggtag accgtttgat cggtgataaa caagagttta cgctgcttgc
    26521 gctcaccaag gcaatctgct aaggttttta aaacatggtc ggtaaacagg atatcataat
    26581 ccttgacccg agaatggaca tgaagtgttt gtggcataag cactcctttc atcagtacta
    26641 ggtaacagag ataacgcagt gatatttaga gcttaatctt gccaaagttc ctgccccaac
    26701 tgttctaatt gcttataatc aatttgctgg gcagcatctg acagcgcatg gtcgggatca
    26761 ggatgaacct ccatcatgat gccattggca ccgacagcct tcgctatctt agcagcaggt
    26821 aagagtaaat ctcgtctgcc tgtcgaatga gacacgtcca caataatcgg taaatctgtc
    26881 ttttgttgaa taatagggac tgccataata tcaagcatat ttctagtctc cacatcgtag
    26941 ccacgaacgc cacgttcgca taagataatg ttagattttc cagtgtcttg taagtaggaa
    27001 agggcgccca aatattcctc gatagtagcc atcaagcctc gtttaaacaa aataggctta
    27061 tcgatgtgtg acaaggtttt taaaaattca aaattttgca tgttgcgggc accaacctga
    27121 atgacatcga gatagtcata ggcttcttct aattggcgct cggacatgat ttcactgacc
    27181 gatagtaagc caaattcctg acaaacctca tgcaagtaac gaattccttg caggccaagg
    27241 ccttgaaaag aagcagcaga tgttcttggt ttataggcgc ctcctctaaa atagttgtaa
    27301 ccaagttttt tggcacttga agctgctaat ctaatgtgat cataagattc aatggaacaa
    27361 ggtccaacaa taaaattatt cttagaacaa gtcttatttt gaaagtcaga cataatgtca
    27421 atcattcgtt tgattccttt actaattctg tatggcgtgc tctatgggat aacgtcttat
    27481 ttaggaatac aaaatctcgt tgttatccat gaagcaccct tagcctgtga aatgatacca
    27541 taaacaagat gaaatagcaa gtaaacccta gattgtctat gttcttagtc atttttatta
    27601 aaaaaggctt actagctata aaaaatactg ccctaaatct tattaggaca gtatttttta
    27661 tagatgccac caccgagaat cgaactcgga acgaagcatt accattgctt tattatacca
    27721 tttaactaca gcggctacct ttttataata ctataagtta ttaattgatg caagcttttc
    27781 ttaaaaatat ttccttaatt gtttatcatc aaaatgagag taccagttat taataacatg
    27841 gcacaagagg cctgccttat ggtataatgg taaagatgat ttcagaaaga ggaagctagg
    27901 catgtcttat gaaaaagaat ttttaaaaga ttttgaagac tgggttaaaa ctcagattca
    27961 agtcaatcag ctagccatgg caaccagtca agaagttgcc caagaagatg gcgatgaaag
    28021 ggctaaagat gcctttattc gttatgaaag taagttagac gcctacgaat ttttactagg
    28081 taaatttgac aattataaaa atggtaaagc ctttcacgat attccagatg agttatttgg
    28141 agcaaggcat tattaaggac aaggagacac gaaatagtgg caaaacaaaa aaatcgtaaa
    28201 aaagcattga agttagaaat gaagcgtaat ggcttgttaa aaaaagcagg tcaagttttt
    28261 gataaggcag ttgaaagtgt tgaaacagct gtggataaaa ccatttctgc tggtaaaaat
    28321 cttgtggaaa aaggcagcca aacggttgaa aacctgacag caagcaagga aagacttact
    28381 cttgatgagt ttgcagggca gccagttgta gctggtatcc gctctgacct tgtcgaaacc
    28441 ctatatgcag aaggcatcca ttcagcgcaa gcttttaagg agtggacaga aaaagacctc
    28501 cttgctctta agggcattgg cccagcaacg gttaaaaaat tggtcgaaaa tggcgcaagc
    28561 tttaaaaaat agtcatcttc tcgttgctta atggctataa atttgttaga atattagcaa
    28621 tcagaggtgc attgagttga tctgttacag agagcggtgg tgttgagaaa tccgcacaga
    28681 tgtcaaggct agtagctgag gatcagaaat cagatatgaa aagtagcaga cccattttat
    28741 ggtttgaaac agtggtggta ccgcggaaat cgtaacacga aattcgtccc tgtctaggtt
    28801 atctaggcag ggattatttg gtttcatttg aaagaagatg atggcttatt gttaaaatgt
    28861 tgttagaagt atataataga aaaactttgc ccaaagtgga aacagaacgg ctgctattac
    28921 gtcaacgaaa aggggacaag gcattgttcc agaagcggct tcagctctga ttgaaattgg
    28981 ttttacctta cttgatttgc ataaaattga gctgggttgc tacgactaca acaagcaaag
    29041 tcaggcagtg gctcgcaaat taggctttac cttagaagcc aatgcccgtg accgtaagga
    29101 cgttcaaggt cgccgctgtg gcgacatgcg ttttgggtta ctgagaagtg agtgggagga
    29161 gcagaaacaa aaatgaacct tatcatcatt ggtgctcaag cgtcgggcaa gatgaccatt
    29221 gggcaagaag ttgccaggca gacaggcatg actttgtttc ataatcacga ttctattgac
    29281 tttgttttac ggtttatgcc ttggtctcaa gagagcactg ccttgattga acgtatccgt
    29341 tttgcttttt ttgaaacctt tgctaagaca ggtcaagaca tgatttttac tattgtaatt
    29401 gacttcaatg atccaaatga tgtagcaatg cttgaaaaga ttcaggctgt ttttcagtct
    29461 tatgaccaag aggttctctt tgtcgaatta aaaacagata tagaggaacg tcttaaacgc
    29521 aatcgcacag aaaatcgtct caaacataaa cctcttaaac gcaacatcga gtggtcagaa
    29581 caagatattc aatccacgat ggcatatgct gtttttaatc cagaagaacc acccaaaacg
    29641 ctcacgcatt accaaaaaat caataatacc cagctcaccg ctgctgaaac agcgcagctt
    29701 attatccaaa aaatgactca tatcaaggag aactaagatg acagaactat cacctaaata
    29761 caatcctgcc gaggttgagg caggtcgcta tcaaaaatgg cttgatgcag atgttttcaa
    29821 gccatcagga gaccaaaaag caaaaccgta ttctatcgtg attccaccgc caaatgtgac
    29881 tggtaagttg cacttaggac acgcctggga cacaacgctt caagatatta tcattcgtca
    29941 aaaacgcatg cagggctttg atacgctttg gttgccaggg atggaccatg cagggattgc
    30001 gacacaggct aaagtagagg agcgtctgcg cgagcagggg atttctcgtt acgaccttgg
    30061 tcgtgacaaa ttcctagaca aggtctggga gtggaaagac gaatatgcca ctacgattaa
    30121 agagcagtgg ggcaaaatgg ggctttctgt ggactacagc cgtgagcgct tcacccttga
    30181 tgagggcttg tcaaaagcag ttcgcaaggt ttttgttgac ctctacaaaa aaggctggat
    30241 ttaccgtggt gaattcatca tcaactggga tcctgctgcc agaactgccc tttctgacat
    30301 cgaggttatc cacaaggatg ttgagggagc tttctatcac atgaactata tgttggaaga
    30361 tggctcacgc gcccttcagg tggcaacaac gcgaccagaa accatgtttg gagacgttgc
    30421 cgttgccgta aatccagaag atcctcgtta caaagatttg attggcaaaa atgttatttt
    30481 accaatcgtg aataaattga ttccaatcgt gggtgacgag catgccgatc ctgaatttgg
    30541 gactggagtt gtgaaaatca cgcctgccca tgatcctaac gacttcgagg ttggtcagcg
    30601 tcataactta ccacaagtca atgtcatgaa cgatgacggt accatgaatg agttagctgg
    30661 cgactttgca ggcatggacc gttttgaagc tcgtcaagct accgttgcta aacttgaaga
    30721 acttggcgcc ctagtaaaca tcgaaaaacg tgttcactca gtaggtcact cagaacgttc
    30781 aggtgcagtc gttgagcctc gtctatccac ccaatggttt gtaaaaatgg atgagcttgc
    30841 taaacaagct atggacaacc aagaaacaga tgaccgagta gatttctacc caccacgctt
    30901 taatgatacc ttccttcaat ggatggaaaa tgtccatgac tgggtaatct cgcgtcagct
    30961 ttggtggggg caccaaattc ctgcttggta caatgcagaa ggtgagattt acgttggcga
    31021 agaagcacca gaaggtgatg gctggactca agatgaagat gttcttgaca cttggttctc
    31081 atctgcttta tggccgttct caactatggg gtggcctgac acagatgtag aagatttcaa
    31141 acgttatttc ccaacctcaa ccttagtaac aggttatgac atcattttct tctgggtgtc
    31201 acgtatgatt ttccaatcgc ttgaatttac cgggcgtcag ccattccaaa atgtcctgat
    31261 tcacggtctt attcgtgacg aagaaggacg caaaatgtct aaatcattgg ggaacgggat
    31321 tgatcctatg gatgtcattg agaagtacgg tgccgatagc ttacgttggt tcctctcaaa
    31381 cggctctgcc ccaggacaag acgtgcgttt ctcatacgaa aaaatggacg cctcttggaa
    31441 tttcattaat aaaatttgga acatttctcg ctatatcctc atgaataacg aagggttgac
    31501 cttggaagaa gctgaaagta atgtggctaa ggtagctgcc tcagaagctg gaaatgtgac
    31561 tgaccagtgg attctccaca atctcaatga aacaatcgct aaagttacgg aaaactttga
    31621 caagtttgag tttggtgtgg caggacacat tctttacaac tttatttggg aagaatttgc
    31681 caactggtat gtggaattga ccaaggaagt gctatatagc gacaacgaag ctgagaaagt
    31741 tatcacgcgc tctgtccttc tttatacttt ggataagatt ttacgtctcc ttcacccaat
    31801 catgccattt gtaacagaag aaatttacgc tcaatatgcc caaggttcta ttgtgacggt
    31861 ggactatcct gtggttcgcc cagcttttga aaatgaagct gcgcataaag gtgtggaaag
    31921 tctgaaagac ctgattcgtg cagttcgtaa tgcgcgcgct gaagttaacg tggcaccatc
    31981 aaaaccaatc accattttgg tgaagacagc agatagtgag ttggaagact tcttcaccag
    32041 caacgtgaac tacatcaaac gtttcaccaa tcctgaaaaa ttagaaatca gctcagccat
    32101 tgctgcgcca gagctggcta tgaccagcat catcacaggt gctgaaatct acttgccact
    32161 agcagatttg cttaacgtgg aagaagaact cgttcgccta gataaagaac tagctaaatg
    32221 gcaaaaagaa ttggacatgg tcggcaaaaa actcggaaac gaacgctttg tagccaacgc
    32281 caaaccagaa gtcgtccaaa aagaaaaaga caaacaagct gactaccaag ctaaatacga
    32341 cgcaacccaa gaacggattg cagagatgaa gaaaattaaa tcatagtgtt tagaacgcag
    32401 ttgaaaaact gtgttctttt cttactaata gagatattag attgaagttt aattatgaga
    32461 aaaacgcttt ctttatttaa agagttatgt tacaatacta ttacaatcat cgtacaaata
    32521 gtgttatttt gaggggcata tgagaatgat tttagcacac tatgactgta aaaaagataa
    32581 aaagcaatct ttagatgagc atttatggca tgtggcctgt tctagtcgac aggaagcatc
    32641 tataattggt caaggagatg tgcttttttt aattggtctt taccacgacc tgggcaaagc
    32701 tgatcgaacc tttcaagata aattattaaa taatccaaat cggcatgttg atcactctta
    32761 tgcaggggca aaatacttat gttctattat tgggcctcat ctaaaaaacc gaggggttga
    32821 taaaaatgag agaatgacat tcaacgaaat ggtggggtat gtcatctctg ctcatcatgg
    32881 gatgtatgat ttatgctact attttgacga tgctgaatat tatggcttta ataagtttaa
    32941 aaatcgtatc aatagagact tagatggtta tcactatcat gaagatatta aagggtacgc
    33001 tctaaaatta gaaaaaaaat tatgtgatta tggctacaaa gatttaaggg agcttattga
    33061 taaagctttt gataattacc aacaagccat gtcttcctta aactggcaag ataagagtga
    33121 gtgggattat tatcagtctt gtatggtgag actttacttg tcactcttaa aaaacgctga
    33181 tattttggac acagtaaatg cctatggcct taagataagt cctatggata aaacagagcg
    33241 atcctttcta aaacactcct atttagcggc cattgaacaa aaatatgcta gctttggaca
    33301 gccaaacaat cagttgaaca ctattcggac agaaatcgct gagcgtgtta aagaaagagg
    33361 taaacgagat tccaagggga tttatcgctt agatttaccg acaggagctg gcaagactaa
    33421 tcttagtatg cgttatgcgt ttcaccaatt agttcatcac gacaaatcaa ggttttttta
    33481 cataacgccc tttctttcgg ttcttgaaca aaatgcttcc gaaattagaa aagttacagg
    33541 tgaccttggc gtcctagaac accattccaa tgtggtgaaa caggctaatg aagatgatga
    33601 tgacaaggac agtttattgt cagcttatct tagtgatagc tgggacagtc aagtagtctt
    33661 gacttctatg gttcaatttt tccaaacact tttcaaaaca aaatcagcta atctgagacg
    33721 tttttcaagt ttgattaata gtgttgtgat tctagatgaa gttcaatccc tgcctattga
    33781 agtcaccact ttgtttaatt taacgatgaa ttttttaaat aaagttatgg atacaaccat
    33841 cgttctttgc acagcaacac aacctgctta tgattcttca gagattgacc atcgtatctg
    33901 ttatggaggg aacttgggag aattagctga aatcgttgag ttaacgattg aagaaaaaca
    33961 gattttttca aggacagagc ttagaaaatt tgatgatagt gatcagaaag ttcacttgac
    34021 tgatgttatt aatcttattc taggtgagga aaactcagtt cttgctattt ttaatacgaa
    34081 aaaaacggtt cataactgct atactatgct aaaagacatt actgatagac cggtctatca
    34141 gctttcgaca aatatgtgtg cgcagcatag acttgacttg attgctaaga tcaaaacgga
    34201 gttacaaaat aatatcccta ttatttgtat tagcacgcaa ttaattgaag caggtgtaga
    34261 tgttgatttt catcgcgtca ttcgttccta ctcagggatt gattctattg ttcaggctgc
    34321 tggacggtgt aaccgagaag gcaaacgaga taaagggcaa gtcactcttg tcaatctgac
    34381 caatgaagag gaaaatattt ctaggctgac agaaataaaa gctaaaaaag aagccacaga
    34441 atctattctt cataagattg ggtctccaat tgatatctca actttaaacc gtgacttttt
    34501 tgagtattat tatgccaata atcagggact gatggattat cctttggaag acaacctatc
    34561 aatctacgac tatttaagcc ttaatattta tcagacggca aataaaaagt tcaaaggtaa
    34621 gttaaaacaa gcttttaaaa cagcaggagc caaaatgaac ctcatcaata atgatatgat
    34681 aggaattctc gtaccttatg gcgaagctga gaaaaaattg gcttatttag aagaattggg
    34741 tatgtctcac tttttatcag cagaagatta tcaaacgata aaatcattac taaaagagtt
    34801 acaacctttt acggttaatg tccgcgagaa cgatcctctc tttgagacaa caaaatctta
    34861 tctaaatggt cagattctgg ttttgacgtc ggagtattat gacacggaaa gaggagttaa
    34921 atacgattca gctagctttt acttctaact caaaacgaaa gcagattaac aaaaggttgt
    34981 tagaggacct tgttaacctg ccaatcatca ttaataatta ttatcaattt agactattta
    35041 ataaaattag attacaaaaa aacagaagga ggaaagtagc ttgtacagat ctagagactt
    35101 ctacgtgaga gtaagtggtc agcgagctct ttttacaaat ccagccacaa aagggggatc
    35161 ggaacgctca tcctattcgg ttccgactag acaggcactg aatggtatcg ttgatgccat
    35221 ctattataag ccgaccttta ctaatatcgt cacagaggtt aaggttatta accagattca
    35281 aaccgaatta cagggtgtca gggctctgtt acatgattat agtgcagatt taagttatgt
    35341 atcctatttg agtgatgttg tttatctgat caagtttcat tttgtttgga atgaagatag
    35401 aaaagatttg aactcagata gacttccagc taaacatgaa gccattatgg agcgttctat
    35461 tcgtaaaggg ggacgtcgag atgtgttttt gggtacaaga gaatgtttag ggcttttaga
    35521 tgatatcagc caagaagagt atgagactac tgtgtcgtat tataatggtg tcaatatcga
    35581 cttgggaatc atgttccatt cctttgccta tccgaaggac aaaaagacac cattaaaatc
    35641 atactttaca aagactgtga tgaaaaatgg agtcattacg tttaaagcac agtctgaatg
    35701 cgatattgtt aacacgcttt ctagttatgc ttttaaagca ccagaggaga taaaatcggt
    35761 taacgatgaa tgcatggagt atgatgccat ggagaaagga gaaaactgat ggattttttt
    35821 acttctctct tgaagactta tgaaaaagca gagctagcag acttggttga tcaccaaaaa
    35881 agaagtaatg agccggtttt actgccgatt tatcatacga gtttaaagtc aaatggcaaa
    35941 aatatcattt cagtgaaact tgacaaagat ggccagtttc acaaggcaga atttatggca
    36001 gataagcaaa tgattatttt tcctgtaacg gctgattctg ttgctaggtc aggtagtcat
    36061 cctgcaccgc atcccctagt cgataaattt gcttattata gtgctgaaat ggggcagtct
    36121 cagtatgatt cttttcataa gcaacttaat aactggattg attattgtga ggagggtgac
    36181 gtcaagaggt ttttaaccat tgttcagcaa tttattttga aaccagaatt tctgacactg
    36241 attctcgatt ctttaattgg tcctgattat caaaataatc aattaaaagt cacattttgt
    36301 gatgccactg gaaaagaaaa attaattgat ttatcagctt gctttttaga attttcaatt
    36361 gatcagttcc agggctttaa aaatgaatcg gtttcgacat ttaaaacttt acaccaatcc
    36421 tatatttctt ttgttgaagc caatcgtgaa aagcttggta tttgtaatat tagtggacga
    36481 gaggaacagc ttaccgataa gcatagaggt ttgatgggga atgctaaaat catctctgtt
    36541 agtaataaaa gagaagctta taaaggacgt tttagagaac gtgaagatgt ttttagtgtt
    36601 ggctatgaaa cttccgaaaa gattcattta atgctcaagt accttttaga aaataaaaat
    36661 accagtactt ggttagggtc ttctcaatat ttaatcaact ggtttagtga tgatttaaca
    36721 aatgatagtc ggttggatat cgtatcacca atctttgatg acggacttga agaagatgat
    36781 gatgacgata cgcctcctgt tataacatta gcaactgaag acaataaaag aattggtaaa
    36841 tcattcatca agggacaaaa attatttgct aatgatgcca cttactacgt tgctattttg
    36901 aataaaacca gcaatgggcg gattgcttta aaatattttc gtcagcttca agcgtcccaa
    36961 ttactcacca atcttaacaa gtggcaggaa acatacagtt gggagtcgcg atctaagttt
    37021 ggaaaaagtc gcttaagaac ccctactttt catgacatcc ttaatgtgtc ctacggggtt
    37081 gatagggatc gcttccttga attagataat gataacttca aaagtgatca aattcaaaag
    37141 ttagtggcaa gtttgattga tggtaaaccg atgccacagt ccattgtcaa aaagttaggt
    37201 aacaatgtta aagaacgaca tcgttaccgt aagcactggt atcaagttga gcaggtctgc
    37261 ttagcaattt tacacaaaca aaatggggag gaattttcac cgatgctaga tcataccaat
    37321 caaaatcgtt cctatctttt tggacgatta ttagcaattt ttgaattaat cgagaccttg
    37381 cgttatggct tggatggaaa caataacgac cgtattacca atgctgaacg ttattggaca
    37441 gcctatactg gacagccaac aaaattgatg atgttattgg aaaataaaat taagccttac
    37501 gaagaaccat tgaaattaaa tcgtcgtggc agttggatga aattagaaaa agaaaaagaa
    37561 gagattttag aactgttaaa tcctctgtta gaaacagaaa caatgggaaa acccttagat
    37621 taccgcttta tttttgggta ttatgctgag aaaaactatt actatacaaa acaaaacacg
    37681 gaagtaacag aaagtgagga gtaaaaagat gttggaacac aaaattgatt ttatggtaac
    37741 tcttgaagtg aaagaagcaa atgcaaatgg tgatccctta aatggaaaca tgcctcgtac
    37801 agatgccaaa ggatatggtg tgatgagtga tgtctccatt aaacgtaaga ttcgtaatcg
    37861 tttgcaagat atggggaagt ctatttttgt gcaagctaat gagcgtattg aagatgattt
    37921 tcgttcactg gaaaaacgct tttcgcaaca ttttacagct aagacacctg acaaagaaat
    37981 tgaagaaaaa gcaaatgcat tatggtttga tgttcgtgct tttggacaag tttttactta
    38041 tctgaaaaaa tcaattgggg tgcgtggacc agtttccatc agtatggcta agtccttgga
    38101 gccaattgtc atttccagcc ttcaaattac gcgtagtacc aatggtatgg aagctaagaa
    38161 taatagtggc cgctcttctg atacgatggg gacaaaacat tttgtagatt atggtgtgta
    38221 tgtacttaaa ggttctatca atgcttattt tgctgaaaag actgtttttt ctcaggaaga
    38281 tgctgaggct attaaagaag ttttggttag cttgtttgaa aatgatgcgt cgtctgcacg
    38341 tccggaaggc tctatgcgag tttgtgaagt cttttggttt acgcattcaa gcaaattggg
    38401 aaatgtttca agtgcgcgtg tctttgactt gttagagtat aatcaatcaa tagaagaaaa
    38461 aagcacttat gacgcttatc agattcatct aaatcaagaa aaattggcta aatatgaagc
    38521 gaaagggtta acgcttgaaa tcctagaagg actctagtat ggtctatgcc gaagatgatt
    38581 atttaatgct atcaggtatt cagcatttcc aattttgtaa acgtcaatgg gcgttgatcc
    38641 atattgagca acaatggctt gataatgaag cgacagcgca tggacaggtt ttacatacta
    38701 aagcagataa cccttacatt aaagaaaaac gaaaagagct tttggtctca cgtgctatgc
    38761 ccatttcttc tgcagaactt ggactttcag gaattatgga tgttgtggaa ttttataaag
    38821 atgatcaagg tgtgtctttg aggggaaaac gtgggaaatg gttaccaaaa gttgtggaat
    38881 acaagcgcgg aaaacctaaa aaagatacca gagatattgt ccagttggtg gctcagacca
    38941 tgtgtttaga agaaacgcta gactgcgaca ttaacgaagg ttgtctttat taccatagtg
    39001 tcaatcaaag agtgattgtt cctatgacat cagctttgcg tcaagaagtg aaggaattag
    39061 ccgcagagat gcatgaggtt tatcagagtc aaatgctacc taaagcagct tattttaaaa
    39121 actgtcagct ttgttcttta gtcgatattt gtaagcccag gttgagtaaa aaaacaagga
    39181 gtgtgtcgcg ttacatcaat gaggctatga ccagtgagga gatggaccta tgaagaagtt
    39241 gctaaatacc ttgtatttga cgcaagaaga tttttatgtc actaaagagg gcgataacat
    39301 tgttatcaag caagaaggta aggttctcaa acggtttccg tttcggatta ttgacggtat
    39361 tgtctgtttt tcttatttgg gtgtgtcgcc tgctttggtg aagttatgta cggagaatca
    39421 gattaattta tcgtttcata caccacaagg gcgtttttgt ggtcgctata ttggttcaac
    39481 caatgggaat gtgttgttgc gtagagaaca ttatcgttta tctgatcgtg aggaatcttt
    39541 ggaatacgca aagcggttta ttttggctaa aatttccaat tcaaggaaat acttgctacg
    39601 ctttaaacga gatcatcgtc aacagattga taccaagctt tttgaggctg ttaatgacga
    39661 attgatatgg gctttagaga tggttcaggc agcagataat aaagactctt taagagggat
    39721 tgaaggccaa gctgctaatc agtattttcg catatttaat gacctggtgt tgacggacaa
    39781 aaaaacgttt tacttccaag gtcggagtaa acgaccaccc ttagattgtg ttaatgccct
    39841 cttgtctttt ggttacagtt tactgacctt tgaatgtcaa tctgccttgg aagctgtcgg
    39901 attagacagt tacgttggtt tctttcacac ggatcgtcct gggcgtgcta gtttagcgct
    39961 tgatttagtt gaagagttcc gctcatatat tgtagatcgt tttgtctttt cattaattaa
    40021 taaaggacaa cttcagaaaa aacactttga ggttaaagaa aatggtagta ttttattgac
    40081 ggaaaatggc agagctattt ttattgattt gtggcagaag cgtaagcata ctgaggtaga
    40141 acatcctttt acaaaagaga aagtaaaact tatgttatta ccctatgtac aagcgcagct
    40201 tttagctaag gccatacgag gagatttaga aagctatcca ccttttatgg tttaggagat
    40261 gttatatgat ggttttagtc acttatgatg taaatacgga aacacctgct ggtagaaaaa
    40321 gattgcgtca tgttgccaaa ctctgtgtgg actatgggca acgtgttcaa aattctgttt
    40381 ttgaatgttc tgtgacaccc gcagaatttg tggatataaa gcaccgctta acacaaatca
    40441 ttgatgagaa aactgatagt attcgctttt atttattggg gaaaaattgg cagaggcgtg
    40501 tggaaacact tggtcgctca gacagctatg acccagataa aggtgtctta ttattgtaaa
    40561 aatctcttgt gcgaagctag ctttcacaga aaaaccttgc ttgctcgcgc aaaaataact
    40621 taaaaaagaa gcgaaatgga gataaaaagg cttaaaacac ctctatgtgt gccatccgtt
    40681 tctctataaa ctgtgtcatt tggcgcagtc tcacccttca tgggtgagtg gattgaaata
    40741 tcgattttgc agataaaagg aaacatagag ttcgtctcac ccttcatggg tgagtggatt
    40801 gaaataattt catctcctgc atcttgatca gttagggtta cgtctcaccc ttcatgggtg
    40861 agtggattga aatatgaact aggacatatt gaacatgatt ctggccaatg tctcaccctt
    40921 catgggtgag tggattgaaa tagattcggc tagatattct aaaaaatcga taaagcgtct
    40981 cacccttcat gggtgagtgg attgaaattc caacttttgt aaaagtagaa tttgctacgt
    41041 ttggtctcac ccttcatggg tgagtggatt gaaatcatta tatgaacaat gcctttgcgg
    41101 aattagttgt ctcacccttc atgggtgagt ggattgaaag atgaacccga cctcattaaa
    41161 agagaggtcg ggtttttaca tgcctttagt caaccattta tctgatagcg tatcaaattc
    41221 aagtttttgc atgttctagt ctatggtttg cgccactagc tttcggtaaa ctaagggtaa
    41281 cttaagagag gagttattta tgatactatc acttgtatta atggtatcgg tttttggggc
    41341 aggcttgtta tcatttttct ctccgtgtat ctttccggtc cttcctgtct atttaggcat
    41401 tttactggat gcagatgatt caaagacgat tactatattt ggtaaaaaac tctattggta
    41461 tggcattgtc aaaactttag cctttatttt tggtctatct actatttttg tgattttagg
    41521 ttacggggct ggttttttag gaaatatcct ttatgcggta tggtttcggt acttactggg
    41581 agccttggtc attatcttgg ggattcatca gatgggcctc attaccatta agagcttaca
    41641 atttcaaaaa tcactgactt tccataataa caagaatcgc aacggtttgt tcaatgcatt
    41701 tatccttggt ttaaccttta gctttggttg gacgccttgt gtgggacctg ttttgagttc
    41761 tgtcctagca ttggtggctt cagggggaaa tggtgcctgg caaggtggcg ttttgatgat
    41821 tatttatact cttggattgg gcattccttt cctactcatc tcttttgcgt caggcattgt
    41881 tttgaaacag tttaacaagc tcaaacccca catactttta ctgaaaaaag taggaggcgt
    41941 tctgattata gtcatgggaa tcttgcttat gacaggaacc ttaaataact tagcacaact
    42001 ttttggataa aggagaaaca caatgaaaaa aggactatta gtaacaactg gtttggcttg
    42061 tctcgggcta ctaactgctt gctcaaccca agacaatatg gctaaaaagg aaataactca
    42121 ggacaagatg agcatggcag ctaaaaagaa agataagatg tcaacatcaa aggacaagtc
    42181 catgatggca gataaatcat ctgataagaa aatgaccaat gatggtccta tggcgcctga
    42241 tttcgaactc aaaggcatcg atggtaagac ctatcgctta tcagagttca aaggtaaaaa
    42301 agtttatttg aaattttggg cctcttggtg ttcaatctgt ctatcaacct tggcagatac
    42361 cgaagacctg gctaaaatgt cagataaaga ctatgtggtc ctaacagttg tctcaccagg
    42421 tcatcaaggg gaaaaatcag aagcagactt taaaaaatgg ttccaaggaa cagactataa
    42481 ggacttacca gtcttgttag atccggatgg caagctattg gaagcttacg gcgtcagatc
    42541 ttacccaaca gaagttttca ttggaagtga tggtgttctt gctaaaaaac acattggtta
    42601 tgccaaaaaa tcagacatca aaaagaccct taaaggtata cattaggaag gaccagctga
    42661 taggcattag aaaaaaggag ttgagtttat ggaacacaaa tggaaattat ggctatttat
    42721 cgttggagtg gtcttgttaa taggcggagc ttctgttctc attcagaaag gtcaagccaa
    42781 ggcttttagt caaggcaagc ctcaactacc agctaaaaaa gtcaaatcca ctaaagtaga
    42841 tcccaatgcc cctgttgatc aaaaaatgat ttacctagct ggtggctgtt tctggggcgt
    42901 tgaagaatat ttttcacaag tggatggcgt cttagatgcg gtgtcaggat atgccaatgg
    42961 tagaggcgat accactaatt accagttaat tcatcagact ggtcatgcag aaaccgttga
    43021 agtggcctat gatgcgaacc gtattagctt gaaagaatta ttactgcatt ttttccgaat
    43081 tattgaccca accagtctta ataagcaagg aaatgatcgc ggtagtcaat accgaactgg
    43141 gacttactat actgacaaag cagatttagc tattattgat gaggtcttca aagaaaaagc
    43201 taaggattac aagaaaaaaa tagtggttga aaaagcacca ttgaaacact ttattaaggc
    43261 agaagactac caccaagatt acctcaagaa aaatccaaat ggctactgcc atattgatat
    43321 taaccaagcg acttatcctg tgattgatga aagcaagtac ccaaaaccaa gtgccactga
    43381 gatcaaagca aaactatctg cagacgaata ccgagtgacg caaaaaaacg aaactgaaaa
    43441 agccttttca aaccgctact gggattcttt tgatgcagga atttacgtag acgttgtgac
    43501 aggagaaccg cttttctctt caaaagacaa atttgaatca ggttgtggtt ggccaagttt
    43561 tagccgcccc atcagcccag atgttattcg ttacaaggaa gataagagtt tcaatatgac
    43621 gagaacagag gtgcgcagcc gttcagggga ttctcatctg gggcatgtct ttactgatgg
    43681 acctaaagac caaggtggcc ttcgttactg catcaatagc ttgtccatta cctttattcc
    43741 aaaagctgat atggaagcta agggctatgg ttatttatta tcatctgtag aatagggtta
    43801 ggcctaaaaa tctgatataa tagaagaaag actaatatga gagggccatg caaggccctt
    43861 tataaagata aggagacacc gtgtgtactc attgttaatt gtagaagacg aataccttgt
    43921 gcgccaaggt attcgttctt tggttgattt tagccagttc aagattgatc gggtcaacga
    43981 agcagaaaat ggccagttgg cttgggactt gtttcagaaa gagccttatg atattgtttt
    44041 gacggatatc aatatgccca aattaaatgg gattcaacta gcagaactca ttaaacagga
    44101 atccccccaa actcatctgg tatttttgac gggctacgat gattttaact atgccttatc
    44161 tgctttgaaa ttaggggcag atgattactt gctcaaaccc ttttccaagg cagatgtaga
    44221 agacatgtta ggaaagctcc ggaaaaaatt agaactttcc aagaaaacag aaaccattca
    44281 ggaattggtt gagcagcctc aaaaagaagt atcagcaata gcaatggcta ttcatgagcg
    44341 gttggcagat tctgatttga ccctaaaaag cttggctcag cagcttggtt ttagccctaa
    44401 ttaccttagc gtcttgatta aaaaagattt agggatgccc tttcaagatt atctggtaca
    44461 agaacggttg aaaaaagcca agcttctctt gttaaccagt aatcttaaaa tctatgaaat
    44521 tgcagagcag gtcggctttg aggacatgaa ttatttttct cagcgcttta agcaactggt
    44581 aggtgttacc ccaagtcagt ataaaaaagg aggtcaggca tgaagcgtta tcccctactg
    44641 gtccagttga tttcgtatgt ctttgtgatc gtaattgccc ttattaccac actgggctta
    44701 ctctattacc agaccagttc tcgtaatatc aggcagttaa ttgaacgtga tactcggcag
    44761 agcattcggc aaagttccca atttattgac gcttacatca agcctcttaa agaaacaact
    44821 tcggtgctgg cgaaaaatac ggaaattcaa gcctttgcta gtcaaattca tcaagaaaat
    44881 gacaagcagg tccttcagct catgaagatg gttcttgcga ccaattccga tttacaagca
    44941 gctgttctgg tgactaagga tggtcgaacg gtgcctacca attctcagtt gaccatgaaa
    45001 acctccagtg acatgatggc agaaccctgg tataaagcag ccatagaccg tcaggccatg
    45061 ccagtcttga ccccagctcg gcaattatcc ctttcttcta aaaaagaatg ggtagtttct
    45121 gtgacccaag aggtagtgga tgctgctggt cataatttag gcgtgctaag acttgatatt
    45181 gcatacccga ccattaaagc gtctttagat cagcttcagc taggccgcca aggctttgcc
    45241 tttattgtga atgataagca tgaatttgtt taccatccca aaaagagtgt ttacagttct
    45301 tctaaggaga tggctgcgat gaagccttat ttagcgattc agaatggtta cactaaggac
    45361 aagacatctt ttgtttacca aaaactcatt cctaacagtc aatggacttt agtgggagtg
    45421 gcgtcactgg atcagttgca ccgggtgcag cgccaaattt tttggtcctt tcttggaaca
    45481 gggcttctag cctgtctgat ttgtggcttt gcaactgtct tagccttacg cagatggatt
    45541 cgtcccattc aacaattgca gcaagttatt cttgctattc aaaaaggaga tcgtcagtta
    45601 cgtgcccaag aaacgggttc tccagaattg acagaccttg cccaacagtt taatgcgctc
    45661 ttagatcaaa ttgatagctt gatggttgcc gttgcggata aggaaaaggc gattgggcag
    45721 tataggttac aagccttggc tagtcagatt aacccgcatt ttctctataa caccttggac
    45781 actattattt ggatggcaga atttaatgac agcaagcgcg tggtagaagt gaccaagtct
    45841 ctagctaagt attttcgttt ggcccttaat caggggaacg aatacattcg tttggcagat
    45901 gaactggatc acgttagcca atacctcttt attcaaaaac agcgctatgg agacaagcta
    45961 agttatgaag tgcaaggctt agatgtctac gcagactttg ttattcctaa gcttatctta
    46021 cagcccttag tagaaaatgc tatctaccat ggcatcaaag aagtcgatcg caaaggcatg
    46081 atcaaggtta cggtatctga tacagctcag catctggtgt tgactgtttg ggataatggt
    46141 aaaggcattg aagactcttc actgaccaat agtcagagct tgttggctag gggaggtgtg
    46201 ggccttaaaa atgttgacca gcggttaaaa cttcactatg gtgaaggcta ccacatgacc
    46261 attcatagcc agtcagacca gttcactgaa atacaattaa cccttcctaa aatgcatgaa
    46321 ttaatggcag acgacacaca ggaaaatgag taaggttaca ggaagtgcat gtcagccatt
    46381 tccctaagtt agggtattgg agataagaac cttagaaagg atatcatgag tttagcatct
    46441 gactatttta gccgacagac ccccatagtt gaaaagctaa tggcttacgg ttttgaaaaa
    46501 cgaaataatg gttacttcta taacgaacgg tttatggaag aagaatttga ggcgcagctt
    46561 aggattgatg aggctggcaa tatctgggat cgggttattg attgtgactt ggaggaggat
    46621 tatttgcctt tacaacaagc agcttggcaa ggaacctata ctgggcaggt tagagcggcc
    46681 tatttggaac ttttggagcg cttatcagta gcctgttttg aagtcactcc ttttcagtca
    46741 atgcaagcta atagacttgc taagcatatc accaaagaat ggtctgaccc aatggactat
    46801 ccttttgaaa agcacccaga ccttgcaacc tatcgggtag ggggcaaatg gtatgctatg
    46861 attttttctc tcttggctga taaattagac cagataccag agagattagt aggacagaca
    46921 tgtgaagtga tgactgtcaa agttaatccc aaggatttac ctcagttact gcagcaagaa
    46981 ggcatttacc cagcgtatca catgtcaaag aaaaactgga ttagtattat tttggatgat
    47041 aaggtaacag atgataagct ttggacgtta gtgactcaaa gtcgtcaact agtcaaccca
    47101 aacggtcttt ctaatcctaa tggtcctggt tattgggtca ttccagctaa tttgaaatac
    47161 tatgatattg atgcagaatt tgcagccaat gatgtgattt tatggaccca aaaagctggt
    47221 attgccgtgg gagactacgt gctgatttat atgacagccc cagtcaaatc cattcgctat
    47281 gcctgccaag ttctagaaac tgatatacct aacgagggtt atcgtaaaaa tcctaacatt
    47341 gacaaactca tgcgtctccg aaaatgccaa cagtacaagg atggtctctt gtcttttgac
    47401 cttatgaaaa aacatggcgt agctgcagtg agagggccaa gacgattaag ccctcaatta
    47461 attgcatttt taaaggaaaa agagtatctt aaagaaaaca actaagggga ctaaaggaga
    47521 ataagagatg gttgaatttg tagcagatca aagtttttgg gaactttttc cagatgccaa
    47581 actaggtgtc gtcttattaa aggatgttca gaatcaagca gagtcaccag aagatgtcaa
    47641 acagctctta gcagatagcc atgagttggt aaaagcttat ttaacagcag acaattttag
    47701 tgataatcag gtggttcaag tctaccgcaa ggcctatcaa cactttaaaa ccaaaaaagg
    47761 agctcgctct agcattgaag cccttctcaa gcgggtgtca aatggtcaaa gcattccgtc
    47821 tattaaccca ctagtggata tttataatgc ggctagtcta cgttttggtt tacctgttgg
    47881 agccgaagat agtgacagtt ttatcggaga tttgcgtctg acgacaacag atggtggtga
    47941 cgacttttac ttgattgggg atgctgacaa caacccaacc ttgccaaatg agctgtgtta
    48001 taaagatgat attggagcgg tttgtcgttg ccttaactgg cgagatggcg aacgcaccat
    48061 ggtgacagaa cacaccaaaa atgcatttct gattatcgaa gctcttgatc aagaaggtca
    48121 aaatagattg caagaagctc ttgacttcat tgaaggcagc gctaaaatgt accttcatgc
    48181 aatcacttct gtgcatgtgt tggataaaga caatccacac gtgccacttt ttacagaaaa
    48241 ctagattagt aggatgagct gtgtaaaata gaaggttaac aaaaaagcga gtaaacgaat
    48301 gtcctgtcat ttaggttttc gtttactcgc tttttgatga gattttactg ctgtcctagc
    48361 aaaaggtgag gatgaggtcg tatatcacat agaggttttc tgttaagcct tatttgttac
    48421 taaagaagaa aggtggcgtg taatgactaa gggtttcgta acaagccttg gcttgctcat
    48481 tgtcttcaca gacaatcaag caagtatcat caccacagac cgttgctaca atttctggaa
    48541 tttgcatggc atctaaaatg gagccaaagg attgggctag tccaggtaac gtttttaaaa
    48601 taatttgatg ttgaactatc tttaacatga ccaaagcgtc ttccatgtaa aaacgaagac
    48661 gatgttccca gcgggtttgt gaaatggcta acgcttcata gtgtgtgtcg tttccagaag
    48721 ttactttgac caggttcaat tccttcatgt cacgtgatag ggtagcctga gtgatcgtga
    48781 tgccattttt ttgcaaccgt tcttgtaatt cttgctgtgt atgtatagtt gtctcagaga
    48841 tgagggaacg tatgagttgg tgacgcgtct ctttcttatt cattgttata atcttccttt
    48901 aacgtaattg ataacaatcc gagtatagca aaaagaggag aaggtgtaaa gtttttgatg
    48961 aaccgcttac aaaatggctt ttttatggta aaataaagat gatgtttaga aaaaggagat
    49021 ttgtgtgatt cgaagagaag attatcaata ccttagaaaa ctaaacgatt ttcgctactt
    49081 ttctattgaa caatttgaca aaattgtagg gcagatggaa ttccgaaaag ctaaaaagga
    49141 ccatatcctt ttttttgagg gagataaaag agataagctt tttctagtga cctctggtta
    49201 tttcaaggtg gagcagtccg atcaatcagg aaccttcatg tatacggatt tcatcagaca
    49261 cggcaccatt tttccttacg gtggcctctt tacagatgat tattaccatt tttcagtggt
    49321 agccatgaca gacgtgactt atttttactt tccagtcgat ttatttgaag agtattcgct
    49381 tgaaaatcgt ctacaaatga aacacttata tagtaagatg tctaagctgc tcgagttaca
    49441 tgagttgagg gtccgaaact tgattacatc aagtgctagt tctcgtgtga ttcaatctct
    49501 ggcgattttg ctggttgaaa tgggtaaaga cagtgatacc ttaccttttc aattaacaac
    49561 aacagatatt gctcaaataa gtgggacgac aagagaaaca gttagccatg tgcttaggga
    49621 tttgaagaag caagaattga ttaccataaa aggtaagtat ttgacttatc tagataaaga
    49681 ctatttttta caat
//
LOCUS       DAUDYX010000007        46591 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.007, whole genome shotgun sequence.
ACCESSION   DAUDYX010000007 DAUDYX010000000
VERSION     DAUDYX010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46591
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.007"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            229..1461
                     /locus_tag="VHD02_000398"
     CDS             229..1461
                     /locus_tag="VHD02_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEQ5497031.1"
                     /translation="MIKRLISLVVIALFFAASTVSGEEYSVTAKHAIAVDLESGKVLY
                     EKDAKEVVPVASVSKLLTTYLVYKEVSKGKLNWDSPVTISNYPYELTTNYTISNVPLD
                     KRKYTVKELLSALVVNNANSPAIALAEKIGGTEPKFVDKMKKQLRQWGISDAKVVNST
                     GLTNHFLGANTYPNTEPDDENCFCATDLAIIARHLLLEFPEVLKLSSKSSTIFAGQTI
                     YSYNYMLKGMPCYREGVDGLFVGYSKKAGASFVATSVENQMRVITVVLNADQSHEDDL
                     AIFKTTNQLLQYLLINFQKVQLIENNKPVKTLSVLDSPEKTVKLVAQNSLFFIKPIHT
                     KTKNTVHITKKSSTMIAPLSKGQVLGRATLQDKHLIGQGYLDTPPSINLILQKNISKS
                     FFLKVWWNRFVRYVNTSL"
     gene            1629..2810
                     /locus_tag="VHD02_000399"
     CDS             1629..2810
                     /locus_tag="VHD02_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEQ5497032.1"
                     /translation="MTKRIILTIFTFICFSMMPLVHAEDVMDITRQAGYTVSEVNRPK
                     SSIVVDANSSDILWQDNIDIPRDPASMSKMFTLYILFEELAKGKITMDTTITATPTDQ
                     AIANIYEISNNNIVAGVAYPIRDLITMTAVPSSNAATVMIANYLSNNDASAFIDRINA
                     TAKQLGMTNTHFSNASGAAAQAFQGYYNPTKYDLSASNITTARDLSKLLYTFLRKYPE
                     IISFTNKSVVHTMVGTPYEEEFHTYNHSLPDNQFGMKGVDGLKTGSSPSAAFNAMITA
                     KRGKTRLITIVMGVGDWSDQNGEFYRHPFVNALTEKGFKDSKTLSKKARQKLEKLVPQ
                     TKKETSPKQQHFKATKKQSYLERVEDFMNHNHTFLLICLAIFIITILLLSLVVFAMGR
                     Q"
     gene            complement(2962..4380)
                     /gene="sufB"
                     /locus_tag="VHD02_000400"
     CDS             complement(2962..4380)
                     /gene="sufB"
                     /locus_tag="VHD02_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEQ5497033.1"
                     /translation="MSDINEKVEPKPIDLGDYQFGFHDDVEPIYSTGKGLSEAVVREL
                     SAAKNEPEWMLEFRLKSLETFNKMPMQTWGADLSDINFDDIIYYQKASDKPARSWDDV
                     PEKIKETFDRIGIPEAERAYLAGASAQYESEVVYHNMKGEFEKLGIIFTDTDSALKEY
                     PDLFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRARALTDATVEWIDGNLGAKTTMKYPSVYLDGPGARGTMLSIAFANAGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKSGGKVDYRGQVTFNKQSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(4373..4852)
                     /locus_tag="VHD02_000401"
     CDS             complement(4373..4852)
                     /locus_tag="VHD02_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEQ5497034.1"
                     /translation="MALSKLNHLYMAVVADHSKRPHHHGQLDGVEAVQLNNPTCGDVI
                     SLTVKFDEDKIEDIAFAGNGCTISTASSSMMTDAVIGKSKEEALALADIFSEMVQGQE
                     NPAQKELGEAELLAGVAKFPQRIKCSTLAWNALKEAIKRSANAQHLTDQNVKEGKNV"
     gene            complement(4839..6065)
                     /locus_tag="VHD02_000402"
     CDS             complement(4839..6065)
                     /locus_tag="VHD02_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ5497035.1"
                     /translation="MLDAKDIKQDFQILNQQVNDEPLVYLDNAATTQKPALVLEALQS
                     YYQEDNANVHRGVHTLAERATHKYEASRQQVADFIHAKSSKEVLFTRGTTTSLNWVAR
                     FAEQVLTPEDEVLISIMEHHANIIPWQQACQKTGARLVYVYLKDGQLDMDDLANKLTT
                     KTRFVSLVHVSNVLGCINPIKEIAKLAHAKGAYLVVDGAQSVPHLAIDVQDLDCDFFA
                     FSAHKMLGPTGLGVLYGKEELLNQVEPLEFGGEMINFVYEQEATWKELPWKFEAGTPH
                     IAGAIGLSAAISYLQRLGMADIHAHEAELIAYVLPKLEAIEGLTIYGPSQPSARSGLI
                     SFNLDDLHPHDLATALDYEGVAVRAGHHCAQPLLSYLGVPATVRASFYIYNTKADCDR
                     LVEAILKAKEFFNGTL"
     gene            complement(6096..7358)
                     /gene="sufD"
                     /locus_tag="VHD02_000403"
     CDS             complement(6096..7358)
                     /gene="sufD"
                     /locus_tag="VHD02_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEQ5497036.1"
                     /translation="MTKEKLVAFSQAHAEPAWLQERRLAALEAIPELELPTIERVKFH
                     RWNLGDGTLTENESLASVPDFIAIGDNPKLVQVGTQTVLEQLPMALIDKGVVFSDFYT
                     ALEEIPEVIEAHFGQALAFDEDKLAAYHTAYFNSAAVLYVPDHLEITTPIEAIFLQDS
                     DSDVPFNKHVLVIAGKESKFTYLERFESIGNATQKISANISVEVIAQAGSQIKFSAID
                     RLGPSVTTYISRRGRLEKDANIDWALAVMNEGNVIADFDSDLIGQGSQADLKVVAASS
                     GRQVQGIDTRVTNYGQRTVGHILQHGVILERGTLTFNGIGHILKGAKGADAQQESRVL
                     MLSDQARADANPILLIDENEVTAGHAASIGQVDPEDMYYLMSRGLNQETAERLVIRGF
                     LGAVIAEIPIPSVHQEIIKVLDEKLLNR"
     gene            complement(7453..8223)
                     /gene="sufC"
                     /locus_tag="VHD02_000404"
     CDS             complement(7453..8223)
                     /gene="sufC"
                     /locus_tag="VHD02_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEQ5497037.1"
                     /translation="MSILEINNLHVSIEGKEILKGVNLTLKTGEVAAIMGPNGTGKST
                     LSAAIMGNPNYEVTQGQILLDGVNILDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAMNAGKADEDKISVRDFITKLDEKMALLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGKDFGAMIITHYQRLLNYITPDLVHV
                     MMDGRIVLSGDAALATRLEKEGYAGIAQDLGIEYKEES"
     gene            complement(8345..9514)
                     /locus_tag="VHD02_000405"
     CDS             complement(8345..9514)
                     /locus_tag="VHD02_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEQ5497038.1"
                     /translation="MFSFTIDYVLVLIGALLMSLFLTPLVRFLAFRVGAVDNPNARRV
                     NKVPMPTSGGLAIFMSFLVASLGLIPIASKGAMFFGQTYFSYILPVVIGATVITLTGF
                     LDDLYELSPKLKMFGILIGAVIVWAFTDFKFDSFKIPFGGPLLVFGPFLTLFLTVLWI
                     VSITNAINLIDGLDGLVSGVSIISLVTMAIVSYFFLPQKDFFLTLTILVLISAIAGFF
                     PYNYHPAMIYLGDTGALFIGFMIGVLSLQGLKNSTAVAVVTPVIILGVPIMDTIVAII
                     RRSLSGQKFYEPDKMHLHHRLLSMGFTHRGAVLVVYGITMLFSLISLLLNVSSRIGGV
                     LLMLGLLFGLEVFIEGLEIWGEKRTPLFNLLKFIGNSDYRQAMLLKWKEKKDLKH"
     gene            complement(9521..10282)
                     /gene="mecA"
                     /locus_tag="VHD02_000406"
     CDS             complement(9521..10282)
                     /gene="mecA"
                     /locus_tag="VHD02_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEQ5497039.1"
                     /translation="MEMKQISETTLKITISMDDLEERGMELKDFLIPQEKTEEFFYSV
                     MDELDLPDNFKDSGMLSFRVTPRKDRLDVFVTKSEINKDINLEDLAEFGDMSQMTPED
                     FFKSLEQSMREKGDVKAHEKLEKIEEIMEDVVEATLANQSEAADPSTNHESEPLDYVH
                     YVLDFSTITEAVAFAKTIDFSIEASELYKGSNCYHMTILLDVQQQPSYFANVMYARLI
                     EHANPGSKTRAYLQEHGLQLMLDGAVEQLQKIELG"
     gene            complement(10428..11267)
                     /locus_tag="VHD02_000407"
     CDS             complement(10428..11267)
                     /locus_tag="VHD02_000407"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEQ5497040.1"
                     /translation="MLIIELLKAIFFGIIEGITEWLPVSSTGHLILVQEFIRLNQDKA
                     FIEMFNIVIQLGAIIAVMLIYFERLNPFQPGKTAREVQLTWQLWLKVVIACIPSILIA
                     VPLDNWFEAHFYFMVPIAIALIVYGIAFIWIEKQNAQQEPAVTDLARMSYKTAFFIGC
                     FQVLSIVPGTSRSGATILGAIILGTSRTVAADFTFFLAIPTMFGYSGLKAVKFFLDGH
                     HLDFAQVLILLVASLTAFVVSLLAIRFLTHYVKKHDFTIFGKYRIVLGSLLLIYSFFK
                     FVF"
     gene            complement(11333..13258)
                     /locus_tag="VHD02_000408"
     CDS             complement(11333..13258)
                     /locus_tag="VHD02_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEQ5497041.1"
                     /translation="MKKILMTLVLCFSLLGIRIKAADVDYSITNYEGQLLLSKENTAR
                     FEQKVTYQFDTSYNGQYISLGRTGHLPAGFAIDQKPKVEVYQNGQQVPVSQEFSDLGD
                     GYRLKLYNAGQAGDKVDVKVIWQLHHLLTAYQDVAELNWTPISDWDKTLEKVSLTVTT
                     PTDIQDSNLWAHRGYYQKKPQVLKEGNSRYQINAKNVSGQLELHAYWDKKALLGKEPV
                     DVSTSKKNKIVALETKISRRRTLLQLLFGKVIPLVEVGFLLSQLIQFTRLKKQFNRYH
                     LANHTDHSYEVPEDLSPLVLTQAIYGQSFAYLSPTASESQKLLIPKGVTFEALVQATL
                     LDLIDQKVLLLTKEEGKAYLEISQLDRVTDEEAAFLDMAFGNKVTLPVDQLFSQYHYD
                     ADTIKQLKKTYKGKKLEQEVRQSSEQVIKAMKKASATITNNVLETIKKLNLPDTYRQM
                     TPAEKRKSHSVQGLGCLLVVLNSSLLIYLTMKGSGLALIYLALMVLMMCLGFYISLKL
                     DQYKKLGIETPEGGVRLHQWQSFKNMIRDIDKFEDVAIEGLVVWNRVLVYATLFGYAK
                     KVERYLKVHRIALPEVYQAVRPGELSMVMYATTPTFVSSLSSATTSSNFSVSSGGGIS
                     GGGGFSGGGGGGGGGAF"
     gene            13456..15024
                     /locus_tag="VHD02_000409"
     CDS             13456..15024
                     /locus_tag="VHD02_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEQ5497042.1"
                     /translation="MKKLILSCLVALALLFGGMSRAQANQYLRVGMEAAYAPFNWTQD
                     DASNGAVPIEGTSQYANGYDVQVAKKVAKAMNKELLVVKTSWTGLIPALTSGKIDMIA
                     AGMSPTKERRNEISFSNSYYTSQPVLVVTANGKYAEAKSLKDFSGAKVTAQQGVWHVN
                     LLTQLKGAKLQTPMGDFSQMRQALTSGVIDAYISERPEAMTAEAADSRLKMITLKKGF
                     AVAESDAAIAVGMKKNDDRMATVNQVLEGFSQTDRMALMDDMVTKQPVEKKAEDAKAS
                     FLGQMWAIFKGNWKQFLRGTGMTLLISMVGTITGLFIGLLIGIFRTAPKAKHKVAALG
                     QKLFGWLLTIYIEIFRGTPMIVQSMVIYYGTAQAFGISIDRTLAAIFIVSINTGAYMS
                     EIVRGGIFAVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFVLTFTVTRILRHIERRFDADTYT
                     TGANQMQIAEVSNV"
     gene            15017..15757
                     /locus_tag="VHD02_000410"
     CDS             15017..15757
                     /locus_tag="VHD02_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ5497043.1"
                     /translation="MSNSIIEIKNLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STLLRSINLLEEPSAGQILFHGEDVLAEHYNLTHYREKLGMVFQSFNLFENLNVLENA
                     IVAQTTVLKRDRAQAEQIAKENLNAVGMTEQYWQAKPKQLSGGQKQRVAIARALSVNP
                     EAMLFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFARDVSDRIIFMDKGL
                     IAEEGSPQQIFENPTQDRTKEFLQRFLK"
     gene            15899..16015
                     /locus_tag="VHD02_000411"
     CDS             15899..16015
                     /locus_tag="VHD02_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEQ5497044.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKRYGKK"
     gene            complement(16241..17251)
                     /gene="gap"
                     /locus_tag="VHD02_000412"
     CDS             complement(16241..17251)
                     /gene="gap"
                     /locus_tag="VHD02_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEQ5497045.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNIEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGNFIKVSAERDPENIDWATDGVEIVLEATGFFAK
                     KEAAEKHLHANGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALHDAFGIQKGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVVTLDKNVSVDEINAAMKAASNDSFGYTED
                     PIVSSDIVGVSYGSLFDATQTKVMEVDGSQLVKVVSWYDNEMSYTAQLVRTLEYFAKI
                     AK"
     gene            complement(17599..19677)
                     /gene="fusA"
                     /locus_tag="VHD02_000413"
     CDS             complement(17599..19677)
                     /gene="fusA"
                     /locus_tag="VHD02_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEQ5497046.1"
                     /translation="MAREFSLAKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQTLHDRLQANAH
                     PIQLPIGAEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPEEYLEQAQEYREKLIEAV
                     AETDEDLMMKYLEGEEITNDELIAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVIA
                     YLPSPLDIPAIKGVNPDTDAEEERPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVMNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     AKVILESIEVPEPVIQLMVEPKSKADQDKMGVALQKLAEEDPTFRVETNVETGETVIA
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLIESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGE"
     gene            complement(20044..20514)
                     /gene="rpsG"
                     /locus_tag="VHD02_000414"
     CDS             complement(20044..20514)
                     /gene="rpsG"
                     /locus_tag="VHD02_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEQ5497047.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFSAIKEATGNDALEVFETAMDNIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            complement(20535..20948)
                     /gene="rpsL"
                     /locus_tag="VHD02_000415"
     CDS             complement(20535..20948)
                     /gene="rpsL"
                     /locus_tag="VHD02_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEQ5497048.1"
                     /translation="MPTINQLVRKPRKSKIEKSDSPALNIGYNSHKKVQTKMAAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            complement(21156..23777)
                     /locus_tag="VHD02_000416"
     CDS             complement(21156..23777)
                     /locus_tag="VHD02_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC10/PgrA surface exclusion domain-containing
                     protein"
                     /protein_id="HEQ5497049.1"
                     /translation="MDLEQTKPNQVKQKIALTSTIALLSASVGVSHQVKADDRASGET
                     KASNTHDDSLPKPETIQEAKATIDAVEKTLSQQKAELTELATALTKTTAEINHLKEQQ
                     DNEQKALTSAQEIYTNTLASSEETLLAQGAEHQRELTATETELHNAQADQHSKETALS
                     EQKASISAETTRAQDLVEQVKTSEQNIAKLNAMISNPDAITKAAQTANDNTKALSSEL
                     EKAKADLENQKAKVKKQLTEELAAQKAALAEKEAELSRLKSSAPSTQDSIVGNNTMKA
                     PQGYPLEELKKLEASGYIGSASYNNYYKEHADQIIAKASPGNQLNQYQDIPADRNRFV
                     DPDNLTPEVQNELAQFAAHMINSVRRQLGLPPVTVTAGSQEFARLLSTSYKKTHGNTR
                     PSFVYGQPGVSGHYGVGPHDKTIIEDSAGASGLIRNDDNMYENIGAFNDVHTVNGIKR
                     GIYDSIKYMLFTDHLHGNTYGHAINFLRVDKHNPNAPVYLGFSTSNVGSLNEHFVMFP
                     ESNIANHQRFNKTPIKAVGSTKDYAQRVGTVSDTIAAIKGKVSSLENRLSAIHQEADI
                     MAAQAKVSQLQGKLASTLKQSDSLNLQVRQLNDTKGSLRTELLAAKAKQAQLEATRDQ
                     SLAKLASLKAALHQTEALAEQAAARVTALVAKKAHLQYLKDFKLNPNRLQVIRERIDN
                     TKQDLAKTTSSLLNAQEALAALQAKQSSLEATIATTEHQLTLLKTLANEKEYRHLDED
                     IATVPDLQVAPSLTGVKPLSYSKVETTPLVQEMVKETKQLLEASARLAAENTSLVAEA
                     LVGQTSEMVASNAIVSKITSSITQPSSKTSYGSGSSTTSNLISDVDESTQRALKAGVV
                     MLAAVGLTGFRFRKESR"
     gene            complement(23768..24607)
                     /gene="purR"
                     /locus_tag="VHD02_000417"
     CDS             complement(23768..24607)
                     /gene="purR"
                     /locus_tag="VHD02_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEQ5497050.1"
                     /translation="MKLRRSERMVVISNYLINNPYKLTSLNTFATKYEAAKSSISEDI
                     AIIKKAFEEANIGDIDTLTGASGGVIFTPSISETEARTIVEDLCQRLSENDRILPGGY
                     IYLSDLLSTPKILQNIGRIIANAFKGEKIDAVMTVATKGVPLANAVANILSVPFVIVR
                     RDLKITEGSTVSVNYASASSDRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTITGMISL
                     LTEFDSTLVGVAVFAENAQSEREQMTFKSLLKVSEIDVKNNNVVVEVGNIFDKWKKER
                     EWT"
     gene            complement(24874..25812)
                     /locus_tag="VHD02_000418"
     CDS             complement(24874..25812)
                     /locus_tag="VHD02_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEQ5497051.1"
                     /translation="MKINQMKKDQLFEGFYLIKSAEVRKTRAGKDFISLTFQDDTGEI
                     SGNLWDAQPYNVEEFTAGKVVFMKGRREVYNGTPQVNQITLRNVRPGEPNDPKDFKEK
                     APVSVEEVRDYLEQMLFKIENATWQRIVRALYRKYDKEFYTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPDLNKSLLFAGIMLHDLAKVIELTGPDNTEYTVRGNLIGHIS
                     LINEEITKVISELQIDDTKEEVIVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALSRVSEGEMTNRIFAMDNRSFYKPNY"
     gene            complement(25802..27073)
                     /locus_tag="VHD02_000419"
     CDS             complement(25802..27073)
                     /locus_tag="VHD02_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEQ5497052.1"
                     /translation="MDLILFLLVLVLLGLGAYLLFKVNGLQHQLAQTLEGNADNLSDQ
                     MTYQLDTANKQQLLELTQLMNRQQADLYQQLTDIRDVLHRSLSDSRDRSDKRLEKINQ
                     QVNQSLKNMQESNEKRLEEMRQIVEEKLEETLKNRLHASFDSVSKQLESVNKGLGEMR
                     SVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTSSQYEREFVTVSGSSERVEYAI
                     KLPGNGQGGYIYLPIDSKFPLEDYYRLEDAYEVGDKLAIEASRKALLAAIKRFAKDIH
                     KKYLNPPETTNFGVMFLPTEGLYSEVVRNAAFFDSLRREENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNADDISKILGNVKLEFDKFGGLLAKAQKQMNTANNTLDQLISTRTNAI
                     VRALNTVETYQDQATKSLLNMPLLEEENNEN"
     gene            complement(27075..27707)
                     /locus_tag="VHD02_000420"
     CDS             complement(27075..27707)
                     /locus_tag="VHD02_000420"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEQ5497053.1"
                     /translation="MTKVALFSGGDLSYFTREFDYFVGIDSGSLFLLENGLPLNMAVG
                     DFDSVSQEAFTDIKEKAELFITAHPEKNDTDTELALKEVFARFPEAEVTIFGAFGGRM
                     DHLLSNIFLPSDPGIAPFMAQIALRDQQNMITYRPAGQHLIHQEEGMTYVAFMAEGEA
                     DLTITGAKFELTQDNFFKKKIYSSNAFIHQPITVSLPSGYLIIIQSKDWS"
     gene            complement(27700..28362)
                     /locus_tag="VHD02_000421"
     CDS             complement(27700..28362)
                     /locus_tag="VHD02_000421"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEQ5497054.1"
                     /translation="MSTLKIAPSILAADYANFASELARIEETDAEYVHIDIMDGQFVP
                     NISFGADVVASMRKHSKLVFDCHLMVVDPERYVEAFAQAGADIMTIHTESTRHIHGTL
                     QKIKAAGMKAGVVINPGTPATALEPLLDLVDQVLIMTVNPGFGGQAFIPECLEKVATV
                     AKWRDEKGLSFDIEVDGGVDNKTIRACYEAGANVFVAGSYLFKASDLVSQVQTLRTAL
                     HD"
     gene            complement(28372..29244)
                     /gene="rsgA"
                     /locus_tag="VHD02_000422"
     CDS             complement(28372..29244)
                     /gene="rsgA"
                     /locus_tag="VHD02_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEQ5497055.1"
                     /translation="MQGKIIKSLAGFYYVESEGQVYQTRARGNFRKRGETPYVGDIVD
                     FSAEDNSEGYILAIHPRKNSLVRPPIVNIDQAVVIMSAKEPEFNSNLLDRFLILLEHK
                     AIHPVVYISKMDLLDSPEEIKAIGRQYQAIGYDFVTSLEELLPLLADKITVFMGQTGV
                     GKSTLLNRIAPELALETGEISDSLGRGRHTTRAVSFYNTHGGKIADTPGFSSLDYDIA
                     NAEDLNEAFPELRRLSHECKFRSCTHTHEPKCAVKAALETGELWPVRYEHYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            complement(29668..30540)
                     /gene="rsmA"
                     /locus_tag="VHD02_000423"
     CDS             complement(29668..30540)
                     /gene="rsmA"
                     /locus_tag="VHD02_000423"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEQ5497056.1"
                     /translation="MRIADYSVTKAVLDRHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DQNVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLVPILADTLRDFDNVQVVNQDIL
                     KADLQTQIKQFKNPDLPIKVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRI
                     SAEPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRDQPLIEVKD
                     EDFFFRVSRLSFVHRRKTLWNNLTSHFGKSEDIKAKLEKGLALADIKPSIRGEALSIQ
                     DFGKLADALKEVGL"
     gene            complement(30654..31244)
                     /gene="rnmV"
                     /locus_tag="VHD02_000424"
     CDS             complement(30654..31244)
                     /gene="rnmV"
                     /locus_tag="VHD02_000424"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEQ5497057.1"
                     /translation="MQRGYLSLTEKINIQEVLVVEGKDDTANLRRFYEVDTYETRGSA
                     ITEEDLERINRLNDLRGVIVLTDPDYNGERIRKLIMAAVPTARHAFLNRNEAVPSSKS
                     KGRSLGVEHANFEDLQKALAHVTQQYDDESYFDIRQTDLIRLGLLMASDSRKRREYLG
                     EKLRIGYANGKQLLKRLELFGITLAEVEEVMETYEE"
     gene            complement(31216..32007)
                     /locus_tag="VHD02_000425"
     CDS             complement(31216..32007)
                     /locus_tag="VHD02_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEQ5497058.1"
                     /translation="MTNKVEKLTIFDTHTHLNVAEFQGHETEELTLAQEMGVAYHNVV
                     GFDQATISGALALADQYPNVYATIGWHPTEAGSYSEAVEESIVSQLSHPKVIALGEIG
                     LDYYWMEDPKEVQIEVFKRQMQLAKDHDLPFVVHTRDALEDTYEVIKAAGVGPRGGIM
                     HSYSGSLEMAERFIELGMMISFSGVVTFKKALDIQEAAQHLPLDKILVETDAPYLTPV
                     PKRGKQNHTAYTRYVVDKIAELRGMTVEEVAKETTANAKRVFKLD"
     gene            32292..33122
                     /locus_tag="VHD02_000426"
     CDS             32292..33122
                     /locus_tag="VHD02_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEQ5497059.1"
                     /translation="MPKNHRLITKIEASLEHMTSLEKGIAHFFITTDLTPQELTASEI
                     VKRLHISQAALTRFAKKCGFTGYRAFAFDYLHSLQESQETFQSIHLELTKRVLMDYDA
                     LINKTYELVNEEKLLNLAKLIDASERVYFFGKGSSGLVAREMKLRFMRLGLICDAYSD
                     TDGFTWANSLVNENCLVFGFSLSGKTNSVITALHQASQRGAKTVLLTTDNQTEFDDSL
                     DIIPVSSTHQLHYGNRVSPQFPLLIMMDIIYAYVLAIDKPHKEKIFKNTIIDKENNDL
                     "
     gene            complement(33233..34171)
                     /locus_tag="VHD02_000427"
     CDS             complement(33233..34171)
                     /locus_tag="VHD02_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEQ5497060.1"
                     /translation="MKHYLAIDIGGTAIKYGLISETGDLLEKEEMATEAYKGGPSILE
                     KVKGLVKTYQDQMDLAGVAISSAGMVNPDKGEIFYAGPQIPNYAGTQFKKEIEETFGL
                     PCEVENDVNCAGLAEAISGSAKDYPVALCLTIGTGIGGCLLFNSQVFHGSSHSACEVG
                     YLHLSDGQFQDLASTTALVQEVALAYGDDISQWDGRRIFEQAKAGDAICIAAISKQVD
                     YLGQGIANICYVVNPNVVVLGGGIMAQKDYLADKLKTALDSYLVSSLAKKTQLKFASH
                     GNNAGILGAYYHFKQKNERSCSFITPEKIENNLASS"
     gene            complement(34193..35107)
                     /locus_tag="VHD02_000428"
     CDS             complement(34193..35107)
                     /locus_tag="VHD02_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEQ5497061.1"
                     /translation="MTDLTKYQGIIPAFYACYDDQGAISPERVRALTQYYIDKGVQGL
                     YINGSSGECIYQSVFDRQLVLENVMAVAKGKLTIINHVACNNTKDSIELAAHSERLGV
                     DAIAAIPPIYFRLPEYAVADYWNAISSAAPHTDFIIYNIPQLAGVALTPSLYKTMLAN
                     KRVIGVKNSSMPVQDIQTFCAIGGDDHIVFNGPDEQFLGGRLMGAAAGIGGTYGAMPE
                     LFLRLNQLIVDKDLEKAKTLQYTINEIIGVLVSAHGNMYGVIKEVLRINEGLDIGSVR
                     SPLAELVEEDRVICQRAAALINQAKETF"
     gene            complement(35119..35781)
                     /locus_tag="VHD02_000429"
     CDS             complement(35119..35781)
                     /locus_tag="VHD02_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEQ5497062.1"
                     /translation="MTSKKQGLLHSLFKLDSKWMRASAALFDLLVFNLLFVLSCLPLL
                     TIGVAKMALYASLLDWREGQVSQLFTTYSSYFKHYFKRGMRLGLIELLIMSICVLDLF
                     LIRNQSGLVFQGFKVLCIAVLFLVVILFLYAYPQAVRKELSLSTLFKRSFLLAGLFFP
                     WSFAFLALICLTIFSLQLSLLTLFGGVSLLAIIGISSLTYLYLIIMESLLRRFPLNND
                     IE"
     gene            complement(35938..36768)
                     /locus_tag="VHD02_000430"
     CDS             complement(35938..36768)
                     /locus_tag="VHD02_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEQ5497063.1"
                     /translation="MTKKKLTASDVLTTVILCILTILFIFPFYWIMTGAFKAQADTIM
                     IPPQWWPKAPTIENFKALVVQNPALKWLWNSVFISVATMFLVCGTSSLAGYALAKKRF
                     YGQWILFSIFIAAMALPKQVVLVPLVRIVNFIGIHDTLAAVILPLVGWPFGVFLMKQF
                     SENIPTELLESAKIDGCGEIRTFFNVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRENLTISLGVATMQAEMATNYGLIMAGAAMAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            complement(36781..37668)
                     /locus_tag="VHD02_000431"
     CDS             complement(36781..37668)
                     /locus_tag="VHD02_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ5497064.1"
                     /translation="MNVNKLKMRETLISYAFLAPVLVFFVIFVLIPMIMGFVTSFFNY
                     SMTDFTFVGFANYARMFQDPIFMKSLMNTLIIVIGSVPVVVFFSLFVAAKTYDKNVVA
                     RSFYRAVFFLPVVTGSVAVTVVWKWIYDPMSGILNYVLKYAHVIEQNISWLGDKHWAL
                     LAIIAILLTTSVGQPIILYIAAMGNIDNSLMEAARVDGATEFQVFWNIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIAIISFAQFKILGNDVEY"
     gene            complement(37772..39091)
                     /locus_tag="VHD02_000432"
     CDS             complement(37772..39091)
                     /locus_tag="VHD02_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ5497065.1"
                     /translation="MNMKKLASLVMLGASVLGLAACGGKSQKEAGASKSDTAKTEITW
                     WAFPVFTQEKAEDGVGTYEKKLIAAFEKANPEIKVKLETIDFTSGPEKITTAIEAGTA
                     PDVLFDAPGRIIQYGKNGKLADLNDLFTKEFTKDVNNDKLIQASKAGDTAYMYPISSA
                     PFYMALNKKMLKDAGVLDLVKEGWTTDDFEKVLKALKDKGYNPGSFFANGQGGDQGPR
                     AFFANLYSSHITDDKVTKYTTDDANSIKAMTKISNWIKDGLMMNGSQYDGSADIQNFA
                     NGQTSFTILWAPAQPGIQAKLLEASKVDYLEIPFPSDDGKPELEYLVNGFAVFNNKDE
                     QKVAASKTFIQFIADDKEWGPKNVVRTGAFPVRTSYGDLYKDKRMEKIAEWTKFYSPY
                     YNTIDGFAEMRTLWFPMVQAISNGDEKPEDALKAFTEKANKTIKKTQ"
     gene            complement(39140..39844)
                     /locus_tag="VHD02_000433"
     CDS             complement(39140..39844)
                     /locus_tag="VHD02_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEQ5497066.1"
                     /translation="MPDKPTKEKLMEQLKGGIIVSCQALPGEPLYSETGGIMPLLAKA
                     AQEAGAVGIRANSVRDIKEIQAITDLPIIGIIKKDYPPQEPFITATMAEVDQLAALNI
                     AVIAMDCTKRDRHDGLDIASFIRQVKEKYPNQLLMADISTFDEGLVAHQAGIDFVGTT
                     LSGYTPYSRQEAGPDVALIEALCKAGIAVIAEGKIHSPEEAKKINDLGVAGIVVGGAI
                     TRPKEIAERFIEALKS"
     gene            complement(40118..40252)
                     /gene="rpmH"
                     /locus_tag="VHD02_000434"
     CDS             complement(40118..40252)
                     /gene="rpmH"
                     /locus_tag="VHD02_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEQ5497067.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLAARRRKGRKVLSA
                     "
     gene            complement(40566..41480)
                     /locus_tag="VHD02_000435"
     CDS             complement(40566..41480)
                     /locus_tag="VHD02_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEQ5497068.1"
                     /translation="MVLFTGKTVEEAIETGLQELGLSRLKAHIKVISKEKKGFLGFGK
                     KPAQVDIEGISDKTVYKADKKATRGVPEDINQKNAPAVNSADVEPEEIKATQKLEAED
                     TKVVPLMSEDSPAQTPSNLAETVTERKVQQPSIPVEESEVPQDPGNDGFSKDIEKAAQ
                     EVSDYVTKIIYEMDIEATVETSNNRRQINLQIETPEAGRVIGYHGKVLKSLQLLAQNF
                     LHDRYSKNFSVSLNVHDYVEHRTETLIDFTQKVAKRVLESGQDYTMDPMSNSERKIVH
                     KTVSSIEGVDSYSEGNDPNRYVVVSLQR"
     gene            complement(41492..42319)
                     /locus_tag="VHD02_000436"
     CDS             complement(41492..42319)
                     /locus_tag="VHD02_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEQ5497069.1"
                     /translation="MRKVLRVKKNIKIARIVPLVLLLVACGRGEVTAQSSSGWDQLVY
                     LFARAIQWLSFDGSIGVGIILFTLTIRLMLMPLFNMQIKSSQKMQDIQPELRELQKKY
                     AGKDTQTRMKLAEESQALYKKYGVNPYASLLPLLIQMPVMIALFQALTRVSFLKTGTF
                     LWVELAQHDHLYLLPVLAAVFTFLSTWLTNLAAKEKNVMMTVMIYVMPLMIFFMGFNL
                     ASGVVLYWTVSNAFQVVQLLLLNNPFKIIAERQRLANEEKERRLRERRARKKAMKRK"
     gene            complement(42285..42644)
                     /gene="rnpA"
                     /locus_tag="VHD02_000437"
     CDS             complement(42285..42644)
                     /gene="rnpA"
                     /locus_tag="VHD02_000437"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEQ5497070.1"
                     /translation="MKKTYRVKCEKDFQAIFKDGKSTANRKFVIYHLNRGQDHFRVGI
                     SVGKKIGNAVTRNAVKRKIRHVIMALGYQLKSEDFVVIARKGVESLEYQELQQNLHHV
                     LKLAQLLEKGFESEEKH"
     gene            complement(43184..43924)
                     /locus_tag="VHD02_000438"
     CDS             complement(43184..43924)
                     /locus_tag="VHD02_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ5497071.1"
                     /translation="MMNIFILEDDFIQQTRIESIVVGILKETRIPCNQLEVFSTPQKL
                     FESIQERGDHQLYFLDIEIGEYTRCGLELAAAIRQKDPNAVIVFVTTHSEFAPISFKY
                     KVSALDFIDKAGGQKQFKEQIEECIRYTYDMMSSRESKDMFLFETPQTRLKLPYKDIL
                     YFATATTPHKVCLWTQTERLEFYGNLSEIQAVAPKLFLCHRSYLVNLDKVVRIDKSKQ
                     LLYFENGDSCMVSRLKMKCLFERWEALR"
     gene            complement(43928..45211)
                     /locus_tag="VHD02_000439"
     CDS             complement(43928..45211)
                     /locus_tag="VHD02_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ5497072.1"
                     /translation="MMVIYSMALFYATIFINSWVIFAKVSAIKLSWKRVSVIGISFVI
                     ANMIFDKVILIDQLFFIIVSLLSAPKKKLFEHMFNGFFTILIVELLFRVIGSFFLPAV
                     LGFSIGQINNNLKLLELCYLFVLPIFYLFSYIFSIDLSLIRFISEDKMKKWVFWMNTA
                     MFSYYFFAHFLVTVQSGFLALYFQYRSILVFIYLAIFIWVIVKLDRFAKDQLSQKLTQ
                     AQNERIAYLENYNQSIEQLYREIRTVKHDSENILISLKDSIDSGDIDLITRVYDTVIQ
                     QSATSMMRTNYEISSLDNIKEAVIRSIMNSKLLEAQYLGIELYIEIPDVIDHLPIKLI
                     DLIVLFTGLVDNAIETAKGSRRPFLSIAYFKQDNKQLFIIENSTKTNRVDIAKRFDAQ
                     QQNSAHFLTVLDSYPQITLSTKSDHYRLRQLLEMR"
     gene            complement(45208..46554)
                     /locus_tag="VHD02_000440"
     CDS             complement(45208..46554)
                     /locus_tag="VHD02_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ5497073.1"
                     /translation="MANSEYMFLLLSIIVYYMTKIYIFSFLSDITLPVWKQLTILALA
                     LFFNQFPYLSPLLIDPLLFLVVLRQETKQLFSLKALFLAVAPSVLVDLLSRFMGTIVI
                     PYLFLSSGIYLGHIIFDLLAYLLIFPSFAIINYMIGKDYKMICQSGYSKRSHNFYQTL
                     LIFVLVYYVDIFVILGFTDPFLHFHHSLFVPTPYKLLFLMFILLLVYLLSYFNHSSKE
                     YLKNELRREQQAYMTNLETYGKHLEKLYRDVRAFQSDYLSRIERLGQAIKSESITQIQ
                     DIYAQTVHEANDYWDDKHYNISKLRKINISSIKSLLSAKIISAEKSGIDLNVEVPDNI
                     KETYIPELDLLLLMSIFCDNAIEAALEAQQPHMSIAYFLLDDYQMFVVTNTTKKKVDI
                     NKIFEEGYSSKGSERGIGLSNAQRILKKYPYLSLRTKSFDKEFSQTLTMPKEEVDR"
BASE COUNT        14098 a         9870 c         8448 g        14175 t
ORIGIN      
        1 aaatgaaaaa ttatctgaat atttcgtaga attttcttta ttttatacat ttatctatat
       61 gaaagcgtta cactcctttt ttaatgaggt ttcaattagt ttaataaaga ttgtcattct
      121 ctcatggtag cgttttatta catcactact cttgtgaggt aagtcattac cttagacaaa
      181 aatatggtat aattaaaaaa ataatacttg agtaaggata atctataaat gatcaaacga
      241 ttaatttccc tagtggtcat cgccttattt tttgcagcaa gcactgttag cggtgaagag
      301 tattcggtaa ctgctaagca tgcgattgcc gttgaccttg aaagtggcaa agttttatac
      361 gaaaaagatg ctaaagaggt tgtcccagtc gcctcagtca gtaagctctt gacaacctat
      421 ctggtttaca aagaagtttc taagggcaag ctaaattggg atagtcctgt aactatttct
      481 aactaccctt atgaactcac tacaaactat actattagta acgttcctct tgataagaga
      541 aaatataccg ttaaagaact tttaagtgcg ttagttgtta ataacgccaa tagccccgct
      601 attgctttag ctgaaaaaat aggcggaacc gaacccaaat ttgttgacaa aatgaaaaaa
      661 caattaagac aatggggcat ttccgatgca aaggtcgtca attcaactgg cttaactaac
      721 cattttttag gagctaatac ttatcctaat acagaaccag atgatgaaaa ttgtttttgc
      781 gccactgatt tagctattat tgccaggcat ctcttattag aatttccaga agtactgaaa
      841 ttatctagca aatcctccac tatttttgct ggacaaacca tttacagtta taattacatg
      901 cttaaaggca tgccttgtta tcgagaaggc gtggatggtc tttttgttgg ttattctaaa
      961 aaagccggtg cttcttttgt agctactagt gtcgaaaatc aaatgagggt tattacagta
     1021 gttttaaatg ctgatcaaag ccacgaggat gatttagcta tatttaaaac aaccaatcaa
     1081 ttgttgcagt accttttaat taattttcaa aaagtccagt taattgaaaa taataaacca
     1141 gtaaaaacgt tatctgtctt agacagtcct gaaaaaactg tcaaacttgt agcccaaaat
     1201 agtttatttt ttatcaaacc aatacataca aagaccaaaa ataccgtcca tattactaag
     1261 aaatcatcca caatgatcgc acctctatca aagggacaag tcttaggtag agcaaccctt
     1321 caagataaac atcttattgg acaaggttat ctggatactc ctccttctat caatcttatc
     1381 cttcaaaaaa acatttctaa aagtttcttt ttaaaggtct ggtggaaccg ttttgtgagg
     1441 tatgtcaata cctctttata gaataacaca atgctttttg tatgacattg actgacagag
     1501 tacttctgca gtctttttca tttttattta agcaaactag gctaatccta acaatttttc
     1561 aaaaaaactt cacattgtat tagcaaagtg atatactgaa aatgcattac atttaaagga
     1621 gtcggaaatt gaccaaaaga attatactaa ctatttttac ctttatttgc ttttcgatga
     1681 tgcctcttgt ccacgccgaa gatgtcatgg atattacaag gcaagcaggc tacacagtta
     1741 gtgaggttaa tcgaccaaaa tcttctattg tcgttgatgc aaatagttca gatattcttt
     1801 ggcaagataa tattgatatt cctagggatc ctgctagtat gtctaagatg tttacgcttt
     1861 acattttatt tgaagagctc gctaagggaa aaattacgat ggacactact attacggcaa
     1921 ctccaaccga tcaagctatc gctaacattt atgagattag caataataat atcgtcgctg
     1981 gagtagctta tcctattcgt gatcttatta ctatgacagc tgtgccttca tcaaatgcag
     2041 caactgttat gattgccaat tatttatcaa acaatgatgc ttctgcgttt atcgatcgta
     2101 ttaatgccac agccaaacaa ttaggcatga ctaacaccca tttttcaaac gctagtggtg
     2161 cagcagcaca agcttttcaa ggttattata atcctactaa atatgattta tctgcttcaa
     2221 atattacgac tgctcgagat ttgtccaagt tactctatac cttcttaaga aaataccctg
     2281 aaattatctc ctttacaaat aaatctgttg tgcacactat ggtaggaacg ccatatgaag
     2341 aagaatttca cacctataac cactctctcc cagataatca atttggtatg aaaggagttg
     2401 atggattaaa aacaggttct agtcccagcg ctgcttttaa cgccatgata actgccaaaa
     2461 gaggcaaaac tcgactcatt actattgtca tgggggtcgg agattggtca gatcaaaacg
     2521 gagagtttta tagacatcct ttcgttaatg ccttgactga gaaagggttt aaagatagta
     2581 agaccttatc caaaaaagca cgccaaaaac tcgaaaaatt agtgccacaa accaaaaaag
     2641 aaacatcacc taaacagcag cacttcaaag ccactaaaaa gcaatcgtac ttggaaagag
     2701 tcgaagattt tatgaatcac aaccatactt tcttgcttat ttgcttagct atctttataa
     2761 ttactatcct tttactaagc ctcgttgtct ttgcaatggg acgccaataa tgaattatca
     2821 aaaacctctg tcaaagcaca gaggtttttg atatgttatc ttacgtgttt aaggcaacta
     2881 aaaactaacc acttggggtt agtactacaa ctcataaaat tctatctcaa agatctttca
     2941 gatggataag agaaaatgca attaaccgac agaaccttcc atctcatagg aaatcaatcg
     3001 attgagttcg accgcgtatt ccattggcaa ttctttggta aaaggctcca caaatcccat
     3061 gacaatcatc tctgtggctt cgctttctga taagccacgg ctcatgagat agtagagttg
     3121 ttcttcagaa atcttagaca ctttggcttc atgttctaac gccacctgtg aattatgaat
     3181 ttcgttaaat gggatcgtat ctgatttaga aatatcgtcc atcaaaatag tatcacattc
     3241 aatatgggaa acggattttt tagattgctt gttaaaggtc acttggccac gataatctac
     3301 tttaccacca gacttggcaa ttgattttga cacaattgat gatgatgtat gtggagcatt
     3361 gtgaatcatt ttagcccctg tatcttggtg ttggcctgcg ttagcaaagg caatagacag
     3421 catggtgcca cgcgcacctg gcccatcaag ataaacagaa gggtacttca tggtggtttt
     3481 agctcctaga ttgccatcaa tccattccac tgttgcatcc gtaagggcac gtgcacgttt
     3541 tgtcactaga ttatacacat tgtctgacca gttttgaatg gtggtataac gcatgtatgc
     3601 accgtcaagc gcaaagattt caacgatagc agcatgtaag ctgttacttg aataagtggg
     3661 agctgtacat ccttcaacat aatgaacact tgctccttca tcaacaataa tcaatgtccg
     3721 ctcaaactga ccagtatttt cattgttaat gcggaaataa gtttgcaaag ggatatctac
     3781 cttgacccct ttaggaacat aaataaaggt accaccagac caaactgctg aattgagggc
     3841 tgctaatttg ttgtctgttg gcggaaccag tttggcaaag tattgtttga acaaatcagg
     3901 atattctttg agggcggaat cggtatctgt aaagataatc cctagttttt caaattcacc
     3961 cttcatgtta tggtaaacca cttctgactc atactgagct gatgctccag caagataagc
     4021 acgttctgct tctggaattc caatacgatc aaaggtttct tttatttttt ctggaacatc
     4081 atcccaagaa cgagctggct tatcagatgc tttttgatag taaatgatat catcaaagtt
     4141 aatatctgat aagtctgctc cccaggtttg catcggcatt ttattaaagg tttccaagga
     4201 ttttaaacga aactccaaca tccactcagg ttcatttttg gcagctgata gttcgcgaac
     4261 cactgcctca ctcaatcctt ttcctgtaga ataaatgggc tctacatcat cgtgaaatcc
     4321 aaattggtag tcccctaagt caattggctt tggctctact ttctcattta tatcagacat
     4381 ttttcccttc ctttacattt tggtccgtga ggtgctgagc attggcactt cgtttaatag
     4441 cttccttgag agcattccaa gctagggtag agcatttgat acgctgtgga aattttgcaa
     4501 ctcctgccaa caattctgct tcacctagct ctttttgagc aggattttcc tgtccttgta
     4561 ccatctctga aaaaatatca gctaacgcga gtgcttcttc tttactttta ccaataacag
     4621 catctgtcat catgcttgat gaagctgtgg aaatggtaca gccgttgcct gcaaaagcaa
     4681 tatcttcaat tttatcttcg tcaaacttaa cggtcaaaga aatcacatca ccacaagtcg
     4741 gattattcag ttgaacagcc tctacgccat ctagttgccc atgatgatgt ggacgtttcg
     4801 aatggtccgc taccacagcc atgtatagat ggttcagttt agagagtgcc attgaaaaac
     4861 tcctttgctt ttagaattgc ttcgactaaa cggtcacaat ctgccttggt gttatagata
     4921 taaaaacttg ctctaacagt tgctggtaca cctaaataac taagaagagg ttgggcgcag
     4981 tggtgccctg ctcttactgc aacaccttca tagtccaagg ctgttgccaa gtcatgagga
     5041 tgcaaatcat ccagattaaa agaaatcaga ccagatcttg cactaggctg gcttggtcca
     5101 tatatggtaa gcccctcaat agcttctaat ttcggcaaaa cataggctat tagttctgct
     5161 tcatgcgcat gtatatcagc catgcctagt ctctgaaggt aagaaatggc tgcgcttagc
     5221 ccaatagcac cagctatgtg aggtgttcct gcttcaaact tccagggcaa ttctttccaa
     5281 gtggcctctt gttcgtaaac aaaattaatc atttctccgc caaattcaag aggctccact
     5341 tgattcaaaa gctcttcttt gccgtaaaga acacccaaac ctgttggccc caacatctta
     5401 tgagctgaaa aagcaaagaa atcacaatcc aagtcttgaa catcaatagc caaatgtgga
     5461 accgactggg caccgtcaac aacaaggtag gctcctttag catgtgccag cttggcaatt
     5521 tctttaatgg gattgatgca accaagaaca ttggagacat gtactaggct aacaaaacgt
     5581 gtttttgtcg tcaatttgtt tgccaaatcg tccatgtcaa gttggccatc ttttaaataa
     5641 acatagacta accttgctcc tgttttttga caggcttgtt gccaaggaat gatattggca
     5701 tgatgctcca taatcgaaat caacacctca tcttctggcg tcaagacctg ctctgcaaac
     5761 cgagcaaccc aattcaaact ggttgttgtt cctctggtaa agagcacttc cttgcttgat
     5821 ttagcatgaa taaagtcagc aacctgctgg cgactggcct catatttgtg cgttgcacgt
     5881 tcagccaagg tatgaactcc tcggtggaca ttagcattat cttcttgata ataggattgc
     5941 aaagcctcaa gaacaagcgc cggtttttgt gtggtggcgg cattatccaa ataaacaagg
     6001 ggttcatcat tgacttgttg atttaagatt tgaaagtctt gtttgatgtc ttttgcgtct
     6061 agcaatacta acctctttct ttttggcagt aggtcttaac gattaagcaa tttctcatct
     6121 aaaaccttaa taatctcttg gtggactgat ggaataggaa tttcagcgat aaccgctcct
     6181 aggaatcctc taataaccaa tcgttctgct gtttcttgat ttagtcctcg actcatcaag
     6241 taatacatat cttcagggtc aacctgaccg atagaagctg catgacctgc tgttacttca
     6301 ttttcatcaa ttaagaggat tggattggca tcggctcttg cttggtcaga aagcatcaaa
     6361 acacggcttt cttgttgagc atcagctccc ttagcgcctt ttagaatatg accaatcccg
     6421 ttaaacgtta aggtgccacg ttccaaaatc acaccatgct gtaaaatatg accaaccgta
     6481 cgttgaccat agttggtcac gcgcgtgtca ataccttgta cctgacgacc acttgaggct
     6541 gcaacaactt tcaaatcagc ttgtgagccc tgaccaatca aatcactgtc aaaatcagca
     6601 atgacattgc cttcattcat cacagctaag gcccaatcaa tgttggcatc cttctctaaa
     6661 cgtcctcgac ggctaatata ggttgtcact gaaggaccta agcggtcgat agccgagaat
     6721 ttaatctggc tgcctgcttg agcaatcact tctacactga tattagcgct gatcttttga
     6781 gtggcattgc caatagattc aaaacgctct aaataggtga acttactttc ttttcctgca
     6841 atcactagaa catgcttgtt aaaaggaacg tcactgtcac tatcttgtaa gaaaatagct
     6901 tcaataggag ttgtgatttc caagtgatca ggaacgtaga gcacggctgc gctattaaaa
     6961 taagcagtgt ggtaggcagc tagtttgtct tcatcaaaag ctaatgcctg accaaaatga
     7021 gcttcaatta cttctgggat ttcctcaagc gccgtataaa aatcactgaa aacaactccc
     7081 ttgtcaatta acgccattgg taactgttct aagactgttt gcgtgcctac ctgaacaagc
     7141 tttgggttat ctccaatagc tataaaatct ggaacactag ctagactttc attttctgtt
     7201 aaggtaccat ctcctagatt ccaacggtga aatttaaccc tttcgatggt tggtaactct
     7261 aattctggta tggcttctaa tgccgctaaa cgccgctctt gcagccaagc aggctcagcg
     7321 tgggcttgcg aaaaagccac tagtttttct tttgtcatct gtcaatcctt tgtctctggt
     7381 ttttaaattg gctgggtata tagaacatca cctagctttt tgtagaaagc taaagtaatt
     7441 gtctccaaaa acttaacttt cttccttata ctcgattccc agatcctgtg caatacctgc
     7501 gtagccttct ttttcaagac gagtagccaa ggcagcatca ccagataaga caatacgacc
     7561 atccatcata acatgaacta ggtctggggt aatgtaattc aacaaacgtt gatagtgggt
     7621 gataatcata gctccaaagt ctttcccacg catctcatta acccctttag agactacttt
     7681 tagggcatca atatcaagac cagaatcaat ttcatctaac aaagcaaatt taggctctaa
     7741 catgagcaat tgaagaattt cattgcgttt tttctcccca ccagaaaaac cttcattgag
     7801 gtaacgctct gccatctctt ctttcatacc taacaaagcc attttctcat caagtttggt
     7861 gataaaatct cgcacggaaa tcttatcctc atctgcctta ccggcattca ttgctgcacg
     7921 cataaattca gcgttggtga tccctggaat ttctgaaggg tattgcatgg cgagaaaaag
     7981 tcccagtctc gcacgctcat caacttctag atcgagaata ttgaccccgt caagcaaaat
     8041 ttgcccttgg gtcacttcgt agttaggatt tcccataata gctgctgaaa gggtagactt
     8101 tcctgtccca tttggcccca taatggcagc tacttcccca gttttaagag ttaggttaac
     8161 ccctttcaaa atctctttac cttctattga aacatgtaaa ttattaatct caagaattga
     8221 catgatacac cccttatttt ctctgtcatc tataccatct caagaaaaga cagtgtcatt
     8281 tgcactgctt tttttataat ggtcactgca tctattatag caaaaaaagc cttactcggc
     8341 ttttttagtg tttcaaatcc ttcttttctt tccatttcag aagcattgct tgacgataat
     8401 cactattacc aataaacttt aacaagttaa acaaaggcgt tcgcttttca ccccagatct
     8461 ccaatccttc aataaaaact tctaaaccaa ataaaagtcc aagcatcaag aggacgccac
     8521 caattcgact agaaacattt aaaagtaaag aaataaggga aaaaagcatg gtaataccat
     8581 aaactactaa gacagctcct cgatgagtaa agcccattga taagagtcga tgatgcaggt
     8641 gcatcttatc cggctcgtag aatttttgac ctgataaact acgtcgaata atagccacga
     8701 tggtatccat aatgggtaca ccaagaataa taacaggtgt caccacagcc acagccgttg
     8761 aatttttcaa tccttgaaga gacaaaaccc ctatcataaa gccaataaag agggctcctg
     8821 tgtcacccaa ataaatcata gctggatggt aattataagg aaaaaatcct gcaatagcag
     8881 aaatcaagac caaaatggtt aacgtcaaaa agaaatcctt ttgaggtaaa aagaaataag
     8941 atacaatagc catggtcact aaactaataa tagagacccc actaaccaaa ccatccaaac
     9001 cgtcaatcaa gttaatagca ttagtgatgg aaacaatcca caggactgtt aagaataagg
     9061 ttaagaaagg accaaaaact aacaacggcc ctccaaaagg aattttgaag ctatcaaatt
     9121 taaagtcggt aaaagcccag acaatcactg caccaattag aataccaaac atctttaatt
     9181 taggacttaa ctcatacaaa tcatctaaaa aaccagtaag ggtaattact gtagcaccaa
     9241 tcacgacagg taaaatataa ctgaagtagg tctgaccaaa aaacatggcg cctttagaag
     9301 caatcggaat aagtcccaaa cttgctacta aaaaagacat aaaaatagct aatccaccac
     9361 tggttggcat cggaacctta ttgacccgtc tggcattggg attgtctact gcacccacac
     9421 gaaaggctaa aaagcgaaca agaggggtta agaataagga cattaacaaa gcccctatca
     9481 gaacaaggac ataatctatt gtgaatgaaa acatactatt ttatcccaac tcaatctttt
     9541 gtaattgctc aacagcacca tcaagcatca attgtaagcc atgctcttgt aaataagctc
     9601 ttgtttttga tccagggttt gcatgctcaa tcaatctcgc atacatgaca ttcgcaaaat
     9661 aagatggttg ttgctgaaca tctaataaaa tagtcatatg gtagcagttg ctccccttat
     9721 ataactctga agcttctatc gaaaaatcaa tcgttttagc aaaagccact gcctctgtaa
     9781 tcgttgaaaa atccaataca taatggacat agtctaaagg ttctgactca tgattagttg
     9841 atggatcagc cgcctcagac tgattagcta gagtggcttc aacaacatct tccattatct
     9901 cttcaatttt ttccaatttt tcatgagctt tgacatctcc cttttcacgc atggattgct
     9961 ccaagctctt gaaaaaatct tccggagtca tttgggacat atcaccaaac tctgctaaat
    10021 cttcaagatt aatatcttta tttatctcag atttggtaac aaaaacatct agacggtcct
    10081 tgcgtggagt cactcgaaaa ctaagcatac cactatcttt aaaattatct ggaagatcga
    10141 gttcgtccat cacagaatag aaaaactctt ctgttttttc ttgaggaatc aagaaatctt
    10201 tcaactccat tcctctttct tctaaatcgt ccatactaat tgttattttt agcgttgtct
    10261 cgctaatttg tttcatttcc attctcaata cctcgcacgc gtagtcattt tattatacta
    10321 gaaatgctgt aatttcgcaa tttatgacta actcctcttc tacaaaagga aatttaaaaa
    10381 gcgacagtcc ccaagatctg tcgcaacatc ctaaacgata tgtaagttta aaaaacaaat
    10441 ttaaagaagc tataaataag taaaaggctt cctaacacaa tacggtattt tccaaaaatg
    10501 gtgaaatcgt gtttcttaac ataatgtgtc aagaacctaa tcgccaataa actaaccaca
    10561 aaagcggtca agctagccac taaaagaata aggacttgtg caaaatcaag gtgatggcca
    10621 tcaagaaaaa atttcacagc ctttaatcca ctatatccaa acatggtagg aatggccaaa
    10681 aagaaggtga aatcagcggc cactgttcga ctagttccta aaataatagc tcctaagatg
    10741 gtagcccctg atcgactagt accaggcaca atacttaaga cttgaaaaca accaatgaag
    10801 aaagctgttt tgtaagacat tctagctaag tcagtgacgg caggttcttg ttgagcattt
    10861 tgtttttcaa tccagataaa ggcaatcccg tagacgatta aagcgatggc aatcggaacc
    10921 ataaaataaa aatgtgcttc aaaccaatta tcaagaggaa ccgctattaa aatagaagga
    10981 atacacgcaa tcaccacttt tagccataat tgccaggtaa gctgtacttc tcttgctgtt
    11041 ttcccaggtt gaaagggatt gagacgttca aaataaatca acataacagc gataatcgcc
    11101 cctagttgaa taacgatatt aaacatttct ataaaagcct tatcctgatt tagtcggata
    11161 aattcctgca ctaaaatgag gtgaccagtg cttgaaactg gtaaccattc tgtgattccc
    11221 tcaatgatgc caaagaaaat ggcttttagc aattcgatta ttaacatgtt ttctccttat
    11281 taaccactct tattgtgatt ttctaaactg tgattaatgc caagcagctt ttttaaaagg
    11341 caccgccgcc gcctcctccg ccaccgccag agaagccacc accaccacta atcccgcctc
    11401 cggaagagac tgagaaattt gaggaagttg tagctgaaga caagctagac acaaaggttg
    11461 gtgtcgtagc atacattacc attgataatt cacctggtcg aacagcttgg taaacctcag
    11521 gtaaggcaat acgatggact ttcaaatagc gttccacttt cttagcgtaa ccaaataagg
    11581 tggcataaac caaaacacgg ttccaaacaa ccaaaccttc aatagcaaca tcctcaaatt
    11641 tgtcaatgtc tcgtatcatg tttttaaagc tctgccattg atgtaaccga acacccccct
    11701 caggtgtttc aatacccaat ttcttgtatt gatctaactt caggctaatg taaaagccaa
    11761 ggcacatcat taacaccatt aaagcaaggt aaatgagggc taacccactt cctttcatag
    11821 tcagatagat caataaacta ctattcagaa caactaacaa gcagcctaac ccctggacac
    11881 tatgactttt tcgtttctct gccggggtca tttgacgata agtatctggc aagtttaact
    11941 ttttgatagt ttctaggaca ttgtttgtaa tagttgccga cgcttttttc atcgccttaa
    12001 tcacctgctc agacgattga cggacttctt gctcgagctt tttacctttg tacgttttct
    12061 ttaattgttt aatggtatca gcatcataat ggtattgact aaacaattga tccaccggca
    12121 atgtcacctt gtttccaaaa gccatatcta agaaggcagc ttcctcatct gtcacacgat
    12181 ctaactgact gatttcaaga taagctttcc cttcttcttt cgttaataaa agcacctttt
    12241 ggtcgatcag atccaacaag gtagcttgaa caagggcctc aaaggtcact cctttaggga
    12301 tcaataattt ctggctttct gatgctgtag gggataaata ggcaaaactc tgtccataaa
    12361 tcgcttgtgt caacacaaga ggcgaaagat cttcagggac ttcgtaactg tgatctgtat
    12421 ggttagccaa atgataacga ttgaattgtt tctttaatct tgtaaactgt attaactgcg
    12481 aaagaagaaa acctacctct accagaggaa tgactttgcc aaatagcagc tgtaacaagg
    12541 tccttcgtct ggaaatctta gtttctaacg ctacaatttt attcttttta ctcgttgaaa
    12601 catcaactgg ctcttttccc aataatgctt ttttatccca ataagcatgc aattccaatt
    12661 gccctgagac attcttggca ttaatctgat agcgactgtt accttctttt aacacttgag
    12721 gtttcttttg gtaatatcct ctgtgagccc atagattaga atcctgaata tcagttgggg
    12781 tagtgacggt caaactcacc ttttctaatg ttttgtccca atcactaatc ggtgtccaat
    12841 tcagctctgc cacgtcttga taagctgtta aaaggtggtg cagttgccag ataaccttga
    12901 cgtcaacctt atcaccagct tggccagcat tataaagttt taaacgatag ccatctccaa
    12961 ggtcactaaa ctcttgacta acaggtacct gttgaccatt ctgatatacc tcaactttag
    13021 gcttttggtc aatagcaaag cccgctggca aatgacctgt ccggcccagc gaaatatact
    13081 ggccgttata agaagtgtca aattgatagg ttactttttg ctcaaaccga gcagtatttt
    13141 cttttgataa cagtaactga ccttcatagt tggtgatgct atagtctaca tcagctgctt
    13201 ttattcgtat ccctaaaaga ctgaagcaga gcacaagcgt catcaaaatt tttttcatgg
    13261 caacatcctc tttctgccct cattttacca tattttaagg agatgacaaa acctaaacca
    13321 agtccttcat cttattttta ctcattattt tgaatatttt tccatatttc aacctattca
    13381 ctgataatat attgaaatag gatgcaaatt gctgtaaaat aggtctgaat actatttttg
    13441 aaacaggaga cctatatgaa aaagctaatc cttagctgtt tggtcgcctt ggcccttctg
    13501 tttggaggaa tgagccgggc tcaagcaaac caatatttaa gagtcggaat ggaagcagcc
    13561 tatgctcctt ttaactggac tcaagatgac gcttcaaacg gggctgtccc tattgaggga
    13621 actagccaat acgccaacgg ttacgacgtc caagtcgcta aaaaagtcgc taaagctatg
    13681 aacaaagaac tgttagtcgt taagacctct tggaccggtt taattccagc attaacttct
    13741 ggaaaaatcg atatgatcgc tgctggtatg agtcctacca aagagcgtag aaacgaaatt
    13801 agcttctcaa acagctacta cactagccaa cctgttctag tcgtaactgc taatggcaaa
    13861 tatgctgagg caaaaagcct caaggatttt tctggagcta aagtaactgc ccagcaaggc
    13921 gtttggcatg tcaatctctt aactcaacta aaaggtgcta agttacaaac accaatgggg
    13981 gatttctctc aaatgcgaca agcccttact tcgggtgtta tcgatgccta tatttctgaa
    14041 cggcctgaag ccatgactgc tgaagctgct gatagccgtt tgaaaatgat cactcttaaa
    14101 aaagggtttg ctgttgctga atcagatgct gctatcgctg tcggaatgaa aaaaaatgac
    14161 gatcgtatgg caactgtcaa ccaagtgctt gaaggatttt ctcaaacgga tcgtatggcc
    14221 ctgatggatg atatggttac caaacaaccc gtggaaaaga aagccgaaga tgctaaagca
    14281 tcatttctag gccaaatgtg ggctattttt aaaggtaact ggaagcaatt cttacgtgga
    14341 actggaatga cccttctgat ttccatggtc ggaaccatta caggtctctt tattggatta
    14401 ttaatcggta ttttccgtac agctcctaaa gctaagcata aagtagccgc cttgggacaa
    14461 aaactctttg gttggttact cactatttat atcgaaatct tccgtgggac acctatgatt
    14521 gttcaatcta tggttatcta ctacggaaca gcgcaagcct ttggtatttc gatcgaccgt
    14581 accctagcgg ctatttttat cgtatctatc aatacgggtg cctatatgag tgaaattgtt
    14641 cgcggtggta ttttcgctgt cgacaaaggt caattcgaag cagcaactgc tcttggcttt
    14701 actcacggac aaaccatgcg taaaatcgtg ctaccacaag ttgttcgcaa cattttacca
    14761 gcaacaggta atgagtttgt catcaatatc aaagatactt ctgttttgaa tgttatctct
    14821 gttgtggaac tttacttctc aggtaatacc gtagccacac aaacctacca atatttccaa
    14881 acctttacga ttatcgccat tatctacttt gttcttacct tcacggtgac acgtattctt
    14941 cgtcatattg aacgccgttt cgatgccgat acttacacca ctggagcaaa ccaaatgcag
    15001 attgcggagg tctcaaatgt ctaactcaat tattgaaatc aaaaacctca aaaaatctta
    15061 cggcagcaat gaagtactca aagatatctc cctttctgtt aacaagggag aagtgatttc
    15121 cattatcggt tcttctggtt ctggaaaatc aaccctcctg cgctcgatca atcttttgga
    15181 agagccaagt gccggtcaaa tcctctttca tggtgaagat gtccttgctg aacactacaa
    15241 cttgactcac taccgtgaaa aactaggcat ggttttccaa tcctttaact tgtttgaaaa
    15301 cctaaatgta ttggaaaatg ctatcgtcgc tcaaaccacc gtcctcaaaa gagaccgtgc
    15361 acaagccgaa caaatcgcta aagaaaacct caatgcggtt ggtatgacag aacaatactg
    15421 gcaagccaaa ccaaaacaat tatcaggggg gcaaaaacaa cgggttgcca ttgccagagc
    15481 cttatctgtc aatccagaag ccatgctctt tgatgaacca acatcagctc ttgatcctga
    15541 aatggttggt gaggtcctta aaaccatgca agacttggca aaatcagggt tgaccatgat
    15601 tattgttacc catgaaatgg aatttgctcg tgatgtttct gaccgtatca tcttcatgga
    15661 taagggattg attgctgaag aaggaagtcc tcaacaaatt ttcgagaatc ctacacaaga
    15721 tcgtaccaaa gaatttttac aacgcttctt aaaataagat aaaggatcca actatttcta
    15781 taccagttgg gtcttttttc aattcaaaaa gaatttgtca taaaatactt taccagttaa
    15841 ctattttatg ctatacttat ttttgttaac gttaataatt tatatttagg aggataatat
    15901 ggcagaaaaa actgacttat cgtcagctta cagaagactg aaaagtccca atattaaaac
    15961 cagaaaacgt gctctgaaga ttattcacga gtacaaacgt tacggtaaaa aataagattt
    16021 tactttttat ctctccaaat ttaagtcaga caaaaaaagg ctaagacacc ccttccatct
    16081 tagccttttt gtatacagtt tattaaggga actattttaa aatatctttt atgctagagt
    16141 ctagaatagt tcaaaacgtt aaaatcatac atttaaggta gtgtttaaag tatacgaaaa
    16201 agaactcagc ataaaccaag ctctctttcg ttataactaa ttatttagca atttttgcga
    16261 agtactcaag agtacgtaca agttgagcag tgtaagacat ttcgttgtca taccatgata
    16321 caactttaac caattgtgat ccgtcaactt ccattacttt agtttgagtt gcgtcaaaca
    16381 atgaaccgta tgatacgcct acgatatctg aagaaacgat tggatcttca gtgtaaccga
    16441 agctatcgtt tgaagcagct ttcatagcag cgttgatttc gtcaacagaa acgtttttgt
    16501 caagagttac aaccaactca gttactgatc cagttggaac aggaacacgt tgtgcagcac
    16561 cgtcaagttt accgttaagt tctgggataa caagaccgat agctttagca gcaccagttg
    16621 agttaggaac gatatttgca gcaccagcgc gtgcacgacg aaggtcacca ccacggtgtg
    16681 gtccgtcaag gatcatttgg tcaccagtgt aagcgtggat tgtagtcata agaccttttt
    16741 gaataccgaa tgcatcgtga agagctttag ccataggagc taaacagttt gtagtacatg
    16801 aagcacctga gataactgtt tcagtaccgt caagaatgtc gtggttagtg ttgaaaacaa
    16861 ctgttttaac atcgtttcca ccaggagctg tgataacaac ttttttagca ccgttagcat
    16921 gtaagtgttt ttcagctgct tcttttttag caaagaaacc agttgcttca agaacgattt
    16981 caaccccatc agttgcccag tcgatgtttt ctggatcacg ttcagcagaa actttaatga
    17041 agtttccgtt tacttcaaat ccaccttctt taacttcaac tgttccatca aaacgacctt
    17101 gagttgtatc gtatttcaac aagtgtgcaa gcatatttgg atctgtaagg tcgttgatac
    17161 gagttacttc aacaccttcg atgttttgaa tacggcggaa tgcaagacgt ccgatacgac
    17221 cgaaaccgtt aataccaact ttaactacca ttagtgattt cctccttatg aaaatcaaaa
    17281 gaatattatt tagagggaat gctccctacc tattgtgaaa agattaactt acagtcatct
    17341 gcaacaacct ttcaacagaa ctattatata actatttcag aaaaaatgca aataaattag
    17401 ctattttcat aaagggcagc ttctttcttc tagccttcaa aacccaccaa atataggaaa
    17461 gggctttctt ttttctatgt tctattctgt aatttatctt tttattggga aagatttcca
    17521 aaacttcaaa ctgtactata acgttctgtt acactaaaaa agaggttagg atagttatcc
    17581 tcaacctctt agtggtggtt attcaccttt gtttttctta atgatttctt cttgaactga
    17641 cttaggaaca tcttcatagt ggtcaaatac catcatgaat gtaccacgtc cttgagtagc
    17701 agaacgaaga actgttgcat aaccgaacat ttcagcaagt ggaacataag cacgaacgat
    17761 ttggctgtta ccatgtgctt ccataccatc aacgcgtcca cgacgagctg tcacgtgtcc
    17821 cataacgtct ccaagattgt cttctggagc tgtaatcgtt acaagcatca tcggctcaag
    17881 gatagctggt tgtgctgatt tagctgcttc tttaagagca agagatgctg cgatcttgaa
    17941 ggcagtttca gatgagtcga catcgtggta tgaaccatcg taaagtttag ctttaacgtc
    18001 aaccattggg taaccagcaa gaacaccgtt tgccatagat tcgatcaacc ctttttcaac
    18061 agctgggata aattcacgag gaaccacacc accaacgata gcattttcga attcgaaacc
    18121 tttaccttct tcatttggtg taaattcaat ccaaacatca ccgaattgac ctttaccacc
    18181 agattggcgt ttgaagaatc cacgagcttg tgtagaagca cggaatgttt cacggtaaga
    18241 tacttgagga gcaccaacgt tagcttcaac tttgaactca cgtttcatac ggtcaacaag
    18301 gacatcaagg tgaagttcac ccataccagc gataactgtt tcaccagttt caacgtttgt
    18361 ttcaacacgg aatgttggat cttcttcagc aagtttttga agggcaacac ccatcttgtc
    18421 ttggtctgct ttagattttg gctcaaccat caactggata actggttctg gaacttcgat
    18481 tgactcaaga ataacttttg ctttttcatc agtcaatgag tcaccagttg ttgtgtcttt
    18541 caaaccaaca gcggcagcga tgtcaccagc gtaaactgtt tcaatttctt gacggctgtt
    18601 tgcgtgcatt tgaaggatac gtccgatacg ctcacgttta ccttttgatg tgttcataac
    18661 gtaagaaccg ctattaagaa cacctgagta aacacggaag aatgtcaaac gacctacgaa
    18721 tgggtcagtc atgatcttga aggcaagagc tgcaaatggc tcttcatcag atgctggacg
    18781 ttcttcttca gcatctgtat ccgggttaac acctttgatt gcagggatgt caagtgggct
    18841 tggaaggtat gcgataaccg catcaagcat caattgaaca cctttgtttt tgaaggcaga
    18901 accacaaagt actgggaaga attcaacgtt gattgttgct ttacggatac cagcaatcaa
    18961 ttcgtcgtta gtgatttctt caccttcaag gtatttcatc atcaagtctt catcagtttc
    19021 agcaactgct tcgattaatt tttcacggta ttcttgagct tgttctaagt attcttctgg
    19081 aatatcttct tcaaggatat ctgtaccaag gtcgttggta tagatttctg ctttcatctt
    19141 gatcaagtca atgataccac ggaaatcatc ttctgcaccg attggcaatt ggattgggtg
    19201 tgcatttgct tgaagacggt catgaagggt ttgtactgag taaaggaagt cagcaccgat
    19261 tttatccatt ttgttggcga aaacgatacg tggaacaccg tattcagttg cttgacgcca
    19321 aactgtttca gtttgaggct caacacctga ttgtgagtca agaacggtta cagcaccatc
    19381 aagaacacgg agagaacgtt gtacttcgat ggtgaagtcc acgtgtcctg gtgtgtcgat
    19441 gatgttaacg cggtgaccat cccattgagc tgttgtagcg gcagaagtaa tagtgatacc
    19501 acgttcttgc tcttgctcca tccagtccat ttgtgatgcg ccttcgtgtg tttcaccgat
    19561 tttatgaatt ttaccagtgt agtaaaggat acgctctgtt gtagttgttt taccagcatc
    19621 gacgtgagcc atgataccga tattacgagt ttttgcaagt gaaaattcgc gagccatgtg
    19681 tttctcctat ttttttagtt tactctttca ttatagcact atttagcaaa aacggatagg
    19741 caggacctac ccgttatcta aatatttgtt ggtaaagata atatctattg cctgtataag
    19801 gcttggtatc ttattcattg aactcaggct aaacgctgtg tgaaaaagat aaactttcct
    19861 agaaacaaag gtttcttcgt caaatttcct attttcactt tgcgttctat acgccttcgt
    19921 atcttattaa ttgagttcaa gctacaaatg tctgccacaa agataaaatt acctagaaac
    19981 tgaagtttct gcgtcaattt ccctattttg gctagcattt gtttgacgct aaaatatctt
    20041 atattaccaa cggaagtgag caaaggcacg gttagcttca gccattttgt gagtgtcttc
    20101 acgtttctta actgatgcac ctgtgttgtt tgcagcatcc atgatttctt tagcaagacg
    20161 atctttcata gtgtgttcac cacgagctcg tgatgcgttt accaaccaac gaagtccaag
    20221 tgttgtacga cgttctggac gaacttcaac tgggacttgg tagttagaac caccgacacg
    20281 gcgtgcgcgt acttcaagta caggcatgat gttgtccata gctgtttcaa atacttcaag
    20341 agcgtcattt cctgttgctt ctttgatagc gctgaaagca tcgtaaacga tagtagcagc
    20401 tgtaccacgt ttaccgtcaa gcataacacg gttgataaga cgtgttacga tttttgagtt
    20461 atataatgga tctggtaata cttcgcgttt aggcgcttga tttttacgac tcattttctc
    20521 ttatccccct tctattatcc ttttggacgt ttcgcaccgt atttagaacg gccttgttta
    20581 cggtcagcaa cacctgcagt atcaagtgcg ccacgaacga tatggtaacg tacccctgga
    20641 aggtctttta cacgtccgcc acgaataaga acaacactgt gctcttgcaa gttgtgtccg
    20701 atacctggga tgtaagctgt aacttcaata aggttgctca aacgtacacg agcgaattta
    20761 cgaagggctg agttaggttt tttaggtgtc attgttccaa cacgagtagc aacgccacgt
    20821 ttttgtgggg cagccatttt agtttgaact tttttgtgac tgttgtaacc aatgttcaaa
    20881 gctggtgaat ctgatttttc aattttagat ttacgtggtt tacgtaccaa ctggttaatt
    20941 gtaggcatct acattctcct gtgttttttt atttttggtt gatatagcac ttggtgacag
    21001 cccatatccg gtgcactttt tgcaacaatt gtcagcacgt ctctgtacac ttttgagaga
    21061 ccaaaagtaa aaagtaccgt ctaatatagt aacacagacg gtacttcatg tcaaatagaa
    21121 tttttccttt gatgcaattg cttatgacga atgcatcacc tagattcctt acggaaccta
    21181 aatcctgtga ggccgacagc tgccaacatg acgactcctg ctttaagagc tctttgagta
    21241 ctttcatcaa catcagaaat gagattgctc gttgtagaag atcctgagcc ataagatgtc
    21301 ttagatgagg gctgagtaat cgaagatgtg attttagaca caatggcatt acttgctacc
    21361 atttcagagg tttggccaac aagcgcttct gctacaagac ttgtattttc agcagctaat
    21421 cttgctgaag cttctaatag ttgtttcgtt tctttaacca tttcttgaac aagcggagta
    21481 gtctctacct tactatatga tagcggtttt acgcccgtaa gagatggagc tacttgcaaa
    21541 tcaggcacag tagctatatc ttcgtctaag tggcgatatt ccttttcatt agctaaggtt
    21601 ttaagcaaag tcaactggtg ttctgtggta gcaatagtag cttctagact gctttgttta
    21661 gcttgtaagg ctgctaaagc ttcttgtgca tttaacaaag atgaggtagt tttagccaaa
    21721 tcttgcttag tattatcaat gcgctcacgt atcacttgaa ggcggttagg attcaattta
    21781 aagtccttta agtattgcaa atgagctttt ttagccacca gtgctgtcac tctggctgcg
    21841 gcttgctctg ctaaggcttc tgtctggtgc agtgcggctt tcaacgatgc tagcttagct
    21901 aatgattgat cacgagtagc ttcgagttgt gcttgttttg ctttagctgc tagtaattct
    21961 gttctcaaag aacctttagt atcatttaat tgtctcactt ggaggtttaa gctgtctgac
    22021 tgcttaagtg tgcttgctaa tttaccttga agttgactta ctttagcttg ggctgccata
    22081 atatcagctt cttgatgaat agccgacaaa cgattttcta atgagcttac ttttcctttg
    22141 atcgctgcaa tagtatcaga tacagtgcct actctttggg cataatcttt tgtacttcca
    22201 acggctttta taggggtctt attaaagcgt tgatggttag caatgttaga ctctggaaac
    22261 attacaaagt gttcattcaa agatcctaca ttgctggttg aaaatccaag gtaaacaggc
    22321 gcattagggt tatgtttatc tacacgtaaa aagttaatag catggccgta tgtatttccg
    22381 tgtaaatgat ctgtaaagag catatacttg atactgtcat aaataccacg tttaatacca
    22441 ttcacagtat gcacatcgtt aaaagcaccg atattctcgt acatgttatc atcatttcga
    22501 atgagccctg acgctccggc agagtcttca ataatagttt tatcatgagg cccaacacca
    22561 taatgccctg atacccctgg ctgtccgtag acaaatgatg gtcttgtatt accatgagtt
    22621 ttcttatagc tggtactaag taatcttgca aattcttgtg atcctgctgt aacagtaact
    22681 ggtggtagac ctaattgtct tcttacacta ttaatcatgt gagctgcaaa ctgcgctagc
    22741 tcattttgca cttctggtgt caaattatcg ggatcaacaa agcgattacg atctgctgga
    22801 atatcttggt attgatttaa ttgattacct ggactagctt tggcaataat ttgatctgca
    22861 tgctctttgt aataattatt gtaactagct gatccaatat aaccactagc ttctaatttt
    22921 ttaagttctt caagaggata gccttgcggt gctttcatgg tattattacc cacaatgcta
    22981 tcttgagtag acggagctga ggatttaaga cgactaagtt ctgcctcttt ttctgctaga
    23041 gcagctttct gagctgccaa ctcttcagtc aattgctttt taactttagc tttttgattt
    23101 tctaagtcag ctttagcctt ctccaattct gagcttaatg cttttgtatt atcattagcc
    23161 gtttgagctg ctttagtgat agcatcagga ttgctaatca tagcattgag cttagcaata
    23221 ttttgttcag acgttttgac ttgttccact aaatcttgag ctcgagtagt ttctgctgaa
    23281 atgctagctt tttgttctga caatgcagtc tcttttgaat gttgatctgc ttgagcatta
    23341 tgaagctctg tttcagtagc tgttaactct ctttgatgtt cggctccttg ggctaatagc
    23401 gtctcctcac tacttgcaag agtattagtg taaatttctt gtgcagaggt taaagctttt
    23461 tgttcattat cttgctgctc ttttaagtgg ttgatttcag cagtagtttt tgtcagagcg
    23521 gtagcaagct ctgtcagttc tgctttttgt tgactgagag ttttttcaac tgcatcaata
    23581 gttgcctttg cctcttgaat tgtttctggt tttggtaaac tatcgtcgtg agtattactc
    23641 gccttcgttt ctcctgaggc tctatcatct gctttgactt ggtgagatac gcctacactg
    23701 gcactcaata aagcaattgt tgaggttaaa gcaattttct gcttaacttg gtttggcttc
    23761 gtttgttcta agtccattct ctctcctttt tccatttatc gaaaatatta ccaacttcta
    23821 caacaacatt gttgtttttg acatcaatct ctgaaacttt cagcaatgat ttgaaggtca
    23881 tttgctcacg ctctgattga gcgttttctg caaacacagc aacaccaact aaagtgctgt
    23941 caaattctgt caagagactg atcattcctg taatcgtccc cccgcccttc aagaaatcat
    24001 ctacaatcaa tacacgacta ttgggtttca aactacgctt tgacagaaac atcttttcaa
    24061 tacgatcact agaagcactc gcataattga cagacactgt cgatccttct gttattttta
    24121 aatcacgtct aacaatgaca aaaggaacac ttaaaatatt agccactgca tttgctaaag
    24181 gaactccttt ggttgcaact gtcatcacag cgtcaatctt ttctccttta aaggcattgg
    24241 caataatacg accaatattt tgtaaaattt taggtgtact cagcaagtct gacaaataga
    24301 tatagcctcc aggtaagatg cgatcattct ctgataacct ttgacacagg tcttcaacaa
    24361 tggtacgcgc ttctgtttct gaaatactgg gggtaaaaat tacgcctcca cttgctcctg
    24421 taagtgtatc gatatcacca atgttagctt cttcaaaagc tttcttgata atagcgatat
    24481 cttctgagat agaagatttt gcggcctcat atttagttgc aaaggtgttt aaactagtta
    24541 atttatacgg attgttaatc aaataatttg aaatgacaac catacgttca cttcgtctta
    24601 atttcatagt ttccccattt ttatgtactt tttgctatta taccataaag ttaataaaaa
    24661 acgaacattc gattaggaaa atattggttt ttttgttctt attaatatca tgcaagaatt
    24721 caatctaaga taaactaatc aacattccta ctgaaaggac attctactta ataaccataa
    24781 caagagccaa aaacacttat gaaaccatca taagtgttaa gcacatcaaa aatcatcaca
    24841 aaagcaaagg aactattact tgagaaacac tgatcaatag tttggcttgt aaaaagaccg
    24901 attatccatg gcaaaaatac gattggtcat ctcgccctcg ctaacacggc taagagctgt
    24961 tgtcatcatc atcatattag catcaatatt gtcaatcata tgaataattt cagcttccat
    25021 gatgcgtgga cgtaccggac taccgtattc taattgcccg tgatggctta aaatcacgtg
    25081 cctaaggaca atgacttctt ctttggtatc atcgatttgt agttctgaaa ttacttttgt
    25141 aatttcttcg ttaatgaggg aaatatgccc aatcaagtta ccacgaacgg tgtattctgt
    25201 attatctggt cctgttaatt ctatgacttt agctaagtca tgtaacataa tacctgcaaa
    25261 cagtagactc ttatttaagt caggatagat atctccaatg ctatctgcta gtctgaccat
    25321 cgtagcagta tggtaggcga gacctgactc aaaagcatgg tggtttgttt tagctgcagg
    25381 ataagtataa aattctttat catacttacg ataaagagcc cttacaattc gttgccacgt
    25441 tgcattttca attttaaaga gcatctgttc caaatagtca cgtacttctt caacactgac
    25501 aggtgccttt tctttgaagt cctttggatc attgggctcc cctgggcgaa catttcgcaa
    25561 ggtgatttga ttaacctgag gggtaccatt atagacctcg cgtcgccctt tcataaacac
    25621 gactttacca gcagtaaatt cttccacatt ataaggttgg gcatcccaca gatttcctga
    25681 aatctcgcca gtgtcatcct gaaaggtaag gctaataaaa tctttaccag ctcttgtctt
    25741 acgaacctct gccgacttaa tgaggtagaa cccttcaaaa agctggtctt ttttcatttg
    25801 attaattttc attattttcc tcttctaata agggcatgtt caagagagat tttgttgctt
    25861 ggtcttgata agtttcaacg gtattcaagg ctcgaacaat ggcatttgtc cttgttgaaa
    25921 tgagctgatc cagcgtatta ttagctgtat tcatttgttt ttgagccttg gcaagcaggc
    25981 cgccaaattt atcgaattct aacttgacat tgcctaaaat tttactgatg tcatcagcat
    26041 ttttttggat attaagggtc ttgaaaccaa cagataagga attgagcaaa gcagacaggg
    26101 tcgaaggacc tgcaaccaca atattttctt cccgacgaag gctatcaaag aaagccgcat
    26161 ttctgaccac ttctgaataa agaccttctg ttggtaagaa cataactccg aaattggtcg
    26221 tctctggggg gttcaagtac tttttatgaa tgtctttggc aaagcgtttg atagctgcta
    26281 gaagtgcttt tcggctagcc tcgatggcca gtttatcacc aacttcgtaa gcatcttcta
    26341 atcggtaata atcttcaaga gggaattttg agtcaatcgg taggtaaata tagccgcctt
    26401 gaccatttcc tgggagctta atcgcatatt ctacgcgttc actagaacca ctaaccgtta
    26461 caaattctct ttcgtactgg cttgatgtca tgatatcctc aatgatttgg cctagttgaa
    26521 gttcgcctaa aatgcctcgt gttttggtat tggacaaaac cttatttaaa gtacccacat
    26581 cttgagccac gctacgcatt tctcccaagc ctttattgac actttctagt tgcttggata
    26641 cagaatcgaa agaggcgtgc agacgatttt ttaaggtttc ttccaatttt tcttcaacga
    26701 tctggcgcat ttcctccaaa cgtttttcgt tagattcttg catatttttg agcgattggt
    26761 tgacctgctg gttaattttt tctaagcgtt tgtcagaccg gtccctacta tcagacaaac
    26821 tacggtgcaa gacgtcacga atatctgtta attgttggta aaggtctgct tgttgtcggt
    26881 tcatcagctg tgttagctct aacaattgtt gcttgttagc tgtatccaac tggtaggtca
    26941 tttggtcaga caaattatcc gcgttgcctt ctagggtttg ggcaagctga tgttgaaggc
    27001 cgttgacttt gaacaacaga taagccccta aacctaagag aaccaagacc aaaaggaaca
    27061 agataaggtc catactaact ccaatcttta ctttgaataa taatcaagta gccacttggc
    27121 aaactaactg taataggttg atggataaag gcattactgg aatagatttt ctttttgaaa
    27181 aaattatctt gcgtcaactc aaatttagca ccagtaatgg tcaagtcagc ttctccttct
    27241 gccataaatg ccacataggt catcccttct tcttgatgga taaggtgctg acctgccggg
    27301 cggtaggtaa tcatattttg ttggtcacgc aaggcgattt gtgccataaa gggagcaata
    27361 ccagggtcgc taggcaagaa aatattggat aagaggtggt ccattcgacc cccaaaagcc
    27421 ccaaaaatgg tcacttccgc ctcagggaaa cgtgcaaaga cctctttaag agctaattca
    27481 gtatccgtat catttttttc tggatgagca gtaataaaaa gctctgcctt ctcttttata
    27541 tccgtaaatg cttcttggga aaccgaatca aaatcaccaa cggccatatt caaaggaagc
    27601 ccattttcca ataaaaacag gctcccacta tcaataccga caaaataatc aaactcacgg
    27661 gtgaaataag aaagatctcc cccagaaaag agggctactt tagtcatgta gggccgtcct
    27721 tagagtctgc acttggctca ctaggtcact ggccttgaaa aggtaagaac ctgccacaaa
    27781 gacattggcc cctgcttcat aacaagcacg aatggtctta ttgtctactc cgccatcaac
    27841 ctcaatgtca aaggacaatc ctttttcatc tcgccatttg gctacggtag ccactttttc
    27901 taaacattct gggataaagg cctgaccccc aaaacctggg ttgactgtca taatgaggac
    27961 ttggtcaact aagtctagca aaggttctag ggctgttgca ggagttcctg gattaatcac
    28021 tacccctgct ttcataccag ctgccttgat tttttgaagg gttccatgaa tatgacgagt
    28081 actttctgtg tgaatggtca tgatatcagc accagcttgt gcaaaagcct caacatagcg
    28141 ttcaggatca accaccatta gatggcaatc aaagaccaat ttactgtgct ttctcatact
    28201 tgccacaaca tctgcaccaa aactgatgtt aggaacaaat tgaccatcca taatgtcaat
    28261 gtgaacgtat tcggcatctg tttcttcaat acgagctagc tcagaagcaa aattagcgta
    28321 atcagctgct agaatagatg gggcaatttt tagagttgac atggagtaga cctactttct
    28381 tttgataact tttttatatg tttcacgacg gttttcaatt tcactgagaa attgcaggta
    28441 atgctcataa cgaactggcc acaactctcc cgtttcaagc gctgccttga cagcacactt
    28501 gggttcgtgg gtatgggtac aggatctaaa cttacactca tggctcagcc ggcgcagctc
    28561 tggaaaagcc tcgttcaaat cttcagcatt agcaatatca tagtctaatg atgagaaacc
    28621 tggcgtatcc gcgatttttc cgccatgggt attgtaaaaa ctcacagctc gggtggtgtg
    28681 gcgaccacgc cctagactat ctgagatttc tcctgtttca agagccaatt cgggggcgat
    28741 tcggttgaga agggttgatt tcccaacacc tgtttgtccc ataaagacgg taattttatc
    28801 agctagcaaa ggcaacaatt cctcaagact ggtcacaaaa tcgtaaccga ttgcctgata
    28861 ctgtcgacca atagccttaa tctcctcggg actatctaat aagtccatct tagagatata
    28921 aaccactgga tgaatggctt tgtgttcaag caagatgaga aagcggtcta gaagattgct
    28981 gttaaattct ggttctttag cagacataat aaccactgct tggtcaatat tgacgatagg
    29041 aggtcttacc aaactgtttt tacgaggatg aatcgctaaa atataacctt cagagttgtc
    29101 ctctgctgag aaatccacga tatctccaac ataaggtgtc tcaccgcgct tcctaaaatt
    29161 accacgcgct cgcgtctgat atacctgtcc ttctgactcc acgtaataaa aaccagccag
    29221 agatttaatg attttacctt gcaactgtgc tcctttgccc tactataata actctattat
    29281 atcagaaaac cgctcccaaa tcagcatttg actctaagtt tcactcgcct taaaaaactg
    29341 tcaagattct ctagaccact ggctatcatt taggtcactg ctttttaaaa atagaatatt
    29401 ttatcaacta aaaatcagat cactgatgat tttttgaggt aggaaacgat gtgaatttta
    29461 agaagacctt tgctatttac gaaaaagagt ctaaccgtac cgctatagtc acgctcttgt
    29521 taattgcccc ttttttccgg ggtattaaaa aactagctct gcactgcgat accttaacgg
    29581 ccagcttcaa taagaaaaat ccccctgaac tgaacatttc tgttttgtct acaagggaat
    29641 ttttttattg tcaccggggg aaagctttta taatccaact tcttttaaag catcagctaa
    29701 tttgccaaaa tcttgaatcg acaaggcctc accacgaata gaaggcttga tatctgctag
    29761 tgccagacct ttttcaagtt tagccttgat gtcttcagac ttcccaaaat ggctagtcaa
    29821 gttattccac agggtcttgc gacgatggac aaaactaagg cgagacactc tgaaaaagaa
    29881 atcttcgtcc ttaacctcaa tcaaaggctg gtcgcgacgc accattttaa ggatagcact
    29941 atcgacattt ggtgcaggta caaagacagt gcgcggcaca atgaaagcaa ccttagcagt
    30001 catgtaatac tgcaccgcaa tggacaagga accataagct ttagtgtttg gctcagccga
    30061 gatacgatct gccacttcac gctgcatcat gaccacaaac tcttggaatg gaattttgct
    30121 ctcaatcaag tgcatgagaa taggcgttgt gatgtagtaa ggtagattag ccaccacctt
    30181 aatgggcaaa tcaggatttt taaactgctt gatttgggtc tgcaaatcag cttttaaaat
    30241 atcttgattg accacttgca cattatcaaa atcgcgcaag gtatctgcca aaatcggcac
    30301 caagcggtca tcaatctcaa aagccatcac ttcagcagca ttttctgcca aaaattccgt
    30361 caaagcccca attccaggac caatctcgat aacattgaca ttctgatcaa tctcagccgt
    30421 gtcaacgatt ttctgtaaaa tattggtatc ggtcaaaaag ttctgaccaa aagatttctt
    30481 aaaggtaaat ccgtgacgat caaggacagc cttggtcacg ctataatctg caattctcat
    30541 atattctctt ctttttatga tcactttctt cttggcattt gaaccactag cctaaacttt
    30601 tttgccaaga tacgccattc agttaatggg gctgttttat tttattgtac tctttactcc
    30661 tcatatgtct ccatcacttc ttccacttct gctagggtaa tgccaaataa ttcgaggcgc
    30721 ttgagcagtt gtttgccgtt ggcatagccg atgcggagct tttcgcccaa gtattccctg
    30781 cgtttacggc tatctgatgc cattaaaagc cctagacgaa tcaggtctgt ttgacgaatg
    30841 tcaaaataag actcatcatc atattgctga gtgacatgag caagtgcctt ttggaggtcc
    30901 tcaaaattgg catgttcaac ccctaaggaa cggcccttac tcttagaact cggaaccgcc
    30961 tcgtttcggt ttaaaaaggc gtgacgagct gttggcacag ctgccataat gagcttacga
    31021 atgcgctcac cattataatc tgggtctgtc aaaacaatga caccacgcaa gtcatttagg
    31081 cggttaatcc gttctaaatc ttcttcagtg atagcagagc ctctggtctc ataggtatcc
    31141 acctcgtaaa aacggcgaag gttagccgtg tcgtccttgc cttctacaac aagaacctct
    31201 tgaatattaa ttttttcagt caagcttaaa taccctcttt gcattagcag tcgtttcttt
    31261 tgcaacttct tccacggtca ttccccgtag ctctgcgatt ttatcaacca cataacgggt
    31321 ataagctgta tgattttgtt tgccacgttt tggaactggt gtaagataag gggcatctgt
    31381 ttccaccaaa atcttatcca aaggcaagtg ctgcgctgcc tcttgaatat caagggcctt
    31441 tttgaaagtg accacacctg aaaaagaaat catcatgcca agttcaatga aacgttcagc
    31501 catctctaat gatcctgagt aagaatgcat gatgcctcca cgagggccaa caccagctgc
    31561 tttgatgacc tcataggtat cttccaaggc atcgcgggtg tgcaccacaa aaggtaaatc
    31621 atggtctttt gctaattgca tttggcgctt aaaaacctct atttgcacct ctttggggtc
    31681 ctccatccaa taatagtcta agccaatttc acctaaggca atgactttgg gatgtgataa
    31741 ttgagagacg atagactcct caactgcttc agaataagag cctgcttctg tcgggtgcca
    31801 tccaatagta gcatacacgt taggatactg gtctgcgaga gcaagtgctc cactaatagt
    31861 ggcctggtca aagccaacca cattatggta ggccacgccc atttcttgag ccaaagtcaa
    31921 ttcttcagtt tcgtgtcctt gaaattctgc tacattcagg tgcgtatggg tatcaaaaat
    31981 agttagtttt tctactttat ttgtcatgat gttattataa cataaagatc ttaaccacat
    32041 cagaaaactt ggtgagaact tctgccgttt aggttgatcg taaaaccctt tgctagctga
    32101 cacttgtcta ttttctactt ttttgacaaa tgacctttaa tagtcggctc ctacataagc
    32161 cctcaaaaat actacaacct actcaaataa agtgggttga acaaatctgt atcacaaaaa
    32221 gggtcgctac cttctgttag cgatctatgc tatactagta gatagttaaa ctgtaaggag
    32281 tgtgctttgc tatgcctaaa aaccaccgcc taattactaa aatcgaagca tcattggaac
    32341 atatgaccag tttagaaaaa gggattgctc atttttttat tacaaccgat ctgacacctc
    32401 aagagttgac cgcatccgag atagttaagc gactacacat ctctcaagcc gccttgactc
    32461 gatttgcaaa aaagtgtggt tttactggtt atcgtgcttt tgcctttgat taccttcaca
    32521 gtcttcaaga atcacaagag actttccaat ctattcactt agaattgaca aagcgcgtgc
    32581 tgatggacta cgatgctctt attaataaaa cctatgagct cgttaatgag gaaaaattac
    32641 tgaacttagc caagctgatc gatgcttctg agcgagtcta tttctttggc aaaggatctt
    32701 ctggactagt agcaagagaa atgaagctac gctttatgcg acttggttta atctgtgatg
    32761 cttattcaga cactgatggt tttacttggg caaatagcct cgtcaatgaa aattgccttg
    32821 tttttggttt ttctttatct ggcaaaacaa actctgtcat cacagcactt catcaagcta
    32881 gtcagcgagg ggccaaaact gtcctcttaa caacagataa tcaaaccgaa tttgacgact
    32941 ccttagatat tattcccgta tcatcaaccc accaactaca ttatgggaat cgtgtgtcac
    33001 cacaatttcc tcttcttatt atgatggaca ttatttatgc ttacgtttta gcgattgaca
    33061 aaccgcacaa agaaaaaatc ttcaaaaaca ctattattga caaagaaaat aacgaccttt
    33121 agactacaat agcaaaaagg gcgttgtttt ctttttaaag ggtcgtcatt cacctccccc
    33181 agtatactta aacaagaact cttcacgtca tatagactca cagccactaa gattagctac
    33241 tcgctaaatt attttcgatt ttctcaggag ttataaatga acaggatcgc tcattctttt
    33301 gtttaaaatg gtagtaagct cctaatatac cagcattatt tccatgacta gcaaacttca
    33361 gctgagtttt cttggcaaga ctagaaacca agtagctatc aagagctgtc tttaatttat
    33421 cagctaaata atctttttgc gccatgatac cgccaccaag taccaccaca tttgggttaa
    33481 caacgtagca gatattagca ataccttgtc ctaagtaatc cacctgctta ctaatggctg
    33541 ctatacaaat cgcatcaccc gctttagcct gctcaaaaat acgacgacca tcccattggc
    33601 taatgtcatc cccataagct aaggccacct cttgaacaag agctgtggta gatgccagat
    33661 cctgaaactg accatctgac aaatgaaggt aaccaacttc gcaagctgaa tgactactac
    33721 catgaaaaac ttgggaatta aacaataaac agccaccgat tcccgtccca attgtcaagc
    33781 aaagagccac aggataatcc ttggcactgc cagaaatagc ttcggctaga ccagcacagt
    33841 taacatcgtt ttccacttca cacggtaacc cgaaagtctc ttcaatctct tttttaaact
    33901 gagttccagc atagttagga atctgagggc cggcataaaa gatttctccc ttatcaggat
    33961 taaccattcc tgcagaagaa atggccactc cagccagatc catttggtct tgataggtct
    34021 ttaccaagcc cttgaccttt tccaaaatag agggaccacc tttataagcc tcagtggcca
    34081 tttcctcttt ttccaacagg tctcctgttt cagaaatcaa gccatacttg atagcagtac
    34141 caccaatatc aattgctaaa taatgcttca tttgttatcc cctcgcatct tattaaaagg
    34201 tttccttagc ctgattaatc agagcagcag cccgttggca aataacccta tcttcctcga
    34261 ccaactcagc taaaggtgat cgaacagaac caatatcaag accctcatta atacgaagta
    34321 cttccttaat aaccccatac atattgccgt gagcagaaac aaggacaccg ataatctcat
    34381 taatagtata ttgaagggtc ttggcctttt ctaaatcctt gtcaactata agttgattca
    34441 gccttaaaaa taattctggc atagccccgt aggtgccacc aatgcctgca gcagcaccca
    34501 tcaagcgccc tcctagaaat tgttcatctg ggccattaaa aacaatatga tcgtctccgc
    34561 caatcgcaca gaaggtttga atatcctgca ctggcatgga tgaattttta acgccaatca
    34621 cgcgcttatt agccaacata gtcttataaa ggcttggggt caaggcaacc cctgccagtt
    34681 gaggaatatt atagatgata aaatctgtgt gaggagcagc cgaagaaatc gcattccaat
    34741 aatcagcaac tgcatattct ggtaaccgga aataaatagg cggaatagct gcaatggcat
    34801 ccacgcccaa acgttctgaa tgagctgcca attcaatact atctttagtg ttgttgcagg
    34861 caacgtgatt aataatcgtc agcttgccct tagcgactgc catgacattt tctaacacca
    34921 attgacggtc aaaaacgctc tggtaaatac actctccaga agagccatta atgtaaagcc
    34981 cctgaacccc tttgtcaata tagtactggg tcaaggcacg cacacgctca gggctaatag
    35041 ctccttggtc atcgtaacaa gcataaaagg caggaataat gccttggtat ttcgtaagat
    35101 ctgtcatagt aatgtccttt attcaatgtc attattaagt gggaatcgcc taagaaggga
    35161 ttccatgata atcaagtaga ggtaagttag gctactgatg ccgatgatag ctagcaaaga
    35221 cacgcctcca aataacgtta acagagacaa ttgtaggcta aatatcgtga gacaaataag
    35281 agccaagaaa gcaaaactcc atggaaaaaa gagtccggct agtaaaaagc ttcgcttaaa
    35341 cagcgtagat agggaaagct cttttctgac ggcctgagga taagcgtaca aaaagagtat
    35401 aactaccaga aataaaacag caatgcaaag tactttaaac ccttgaaaaa ctaggcctga
    35461 ttggtttcga attaagaaaa gatctaagac acaaatactc attatcaaaa gctcaataag
    35521 acctaggcgc atccctcttt taaaataatg tttaaaataa gagctataag tagtaaaaag
    35581 ctgactaact tgaccttctc gccaatccaa caaactcgca tacaaagcca ttttagcaac
    35641 cccaatcgtt agaaggggaa ggcaggacaa gacaaataag agattaaaca cgagcaaatc
    35701 aaacagtgct gcgctagccc tcatccattt actatcaagc ttgaagagag agtgtaaaag
    35761 tccctgtttt ttcgatgtca aatcacaact tcctttctag aaatacgctt acaaacaaaa
    35821 gctaattccc ctcttaaacg tttaacacat gacttgcttc ccttttgact tatctatcta
    35881 tgcacctaaa ttaagaggca acctatagat taagcttagt gacacgcttt atttagtcta
    35941 acctttcaca gcacccatag taatgccttg ggtaaatgat ttttggaaaa caagaaagac
    36001 cgttacaata ggcactgcag ccatggcagc ccctgccata atcaagccat aattagtagc
    36061 catttcagct tgcatagtgg caaccccaag tgaaatcgtt aaattttccc gagaggttaa
    36121 catcaccaac tgcataaaat aatcgttcca agtattgata aaggtaaaaa tcgctagcgc
    36181 tgcaaagcct ggtttgacaa tagggaaagc cacattgaaa aaggtacgaa tttcgccaca
    36241 tccatcaatc ttagcggatt ccaataactc ggttggaata ttttctgaga actgtttcat
    36301 aaggaaaaca ccaaatggcc agcccacaag aggcaaaata acagccgcca aagtatcatg
    36361 gattccaata aaattaacaa tccgcacaag aggcactaga acaacctgtt ttggcaaggc
    36421 catagcagca ataaaaatcg aaaacaaaat ccattgtcca taaaaccgct ttttggccaa
    36481 ggcatagcca gccaaagagg aagtgccaca aaccaagaac atggtcgcca ccgaaataaa
    36541 gacactattc cacaaccatt ttaaggctgg attttgcact accaaggcct taaaattttc
    36601 aatagtaggt gcctttggcc accattgcgg tggaatcata atggtatcgg cttgagcctt
    36661 aaatgctcct gtcataatcc aataaaatgg gaaaataaac aaaatggtca agatgcatag
    36721 aataacagta gtcagaacat ccgatgcggt tagtttcttt ttcgtcatct taatatcctc
    36781 ctaatattcc acatcattac ctaaaatttt aaattgagca aagctgatga ttgcaatcat
    36841 gactgccaag aacacaccca ttgtgttggc atagccgtat tcagagagct taaaggcttt
    36901 ttcatacaaa tagtacatga gggtactagt tgaataattt ggccctccag acgtcaagag
    36961 ttgaatcaaa gcaaaacatt ggaaggaatt aatggttgtt atgacagcaa tatataaggt
    37021 tgttggcagg agacttggcc acttgatatt ccaaaacacc tgaaattctg tggcgccgtc
    37081 tacacgcgca gcttccatca aagaattatc aatatttccc atagcagcaa tatatagaat
    37141 aataggctgc ccaacagatg tggttaataa aatagcaata atagctaaca aagcccagtg
    37201 cttatctccc agccaactaa tgttttgttc aatcacatga gcatatttta agacataatt
    37261 caaaatccct gacatcggat catagatcca tttccaaacc accgtcaccg caacacttcc
    37321 ggtaacaaca ggcagaaaga aaactgcccg gtaaaacgaa cgcgccacta catttttgtc
    37381 gtaggtttta gccgccacaa agagggaaaa gaaaactaca acaggtaccg aaccaataac
    37441 aataatcaag gtgttcataa gagacttcat gaaaatggga tcttgaaaca tcctagcata
    37501 attagcaaaa ccaacaaagg taaaatccgt catggaataa ttgaaaaaac tcgtcacaaa
    37561 gcccataatc atcggtatca agacaaaaat cacaaagaaa accaagaccg gagctaagaa
    37621 agcgtatgag atgagcgtct ctctcatttt taatttattg acgttcactt gaaatacctc
    37681 tttttctatt aatctctcct aaaaaaggaa aggctccttt ccagtgacca tttgacatgt
    37741 gccgtgaaaa gaagccactt gttctgaaca cttattgtgt ttttttgatt gtcttgtttg
    37801 ctttttcagt gaaggctttc aaagcatctt ctggcttttc atcaccatta gagatggctt
    37861 gaaccattgg gaaccaaaga gttctcattt cagcaaaccc atcaatcgtg ttgtagtatg
    37921 gtgagtagaa ttttgtccat tcagcaattt tttccattcg tttgtctttg taaagatccc
    37981 cataagaagt tcttacaggg aaagcacctg tacgaacaac atttttaggt ccccattctt
    38041 tatcatcagc gataaattgg ataaatgtct tagaggcagc gactttttgt tcatctttgt
    38101 tattaaagac cgcaaaacca tttacgaggt attctagttc tggtttgcca tcatctgatg
    38161 ggaatgggat ttcaaggtaa tccactttac tagcttctaa caatttagct tggatgccgg
    38221 gttgtgcagg cgcccaaaga atggtaaagg aagtttggcc attcgcaaag ttttgaatat
    38281 cagctgaacc gtcgtattga gagccattca tcattaaacc atctttaatc caattggaaa
    38341 ttttagtcat ggctttaata gagttagcat catctgtggt gtatttggtt actttgtcat
    38401 cggtaatatg actactataa aggttagcaa agaaggcacg tggtccttgg tcaccacctt
    38461 gaccattagc aaagaaagaa cctgggttat atcctttgtc cttcaatgct ttcaagactt
    38521 tttcaaaatc atctgttgtc cacccctctt tcacaaggtc aaggacgcca gcatctttga
    38581 gcattttttt attcaacgcc atgtagaatg gagcagagct gataggatac atgtaagctg
    38641 tatctcctgc ttttgaggct tgaatcaatt tgtcattgtt aacatctttg gtaaactctt
    38701 ttgtgaacaa gtcattcaag tcagccaatt tcccattttt accatattga atgatacgac
    38761 ctggagcatc aaagaggaca tcaggagctg ttccagcttc aatagcagtt gtgattttct
    38821 caggaccaga tgtaaaatca atggtttcca attttacctt gatttctgga ttagctttct
    38881 caaaggcagc aatcaatttc ttctcatagg ttcccacacc atcttcagct ttttcttgcg
    38941 tgaagaccgg gaaagcccac catgtgattt ctgtcttagc agtatcagat ttacttgcac
    39001 cagcctcttt ctggctcttt ccgccacaag ctgctagtcc taaaacagat gcaccaagca
    39061 tcactaatga agctaacttc ttcatgttca tcgtaaaatc tccttttttt gatgtgtgtt
    39121 tctagtttga caaccactat taggatttga gcgcttcgat aaaacgctct gcaatttcct
    39181 taggccttgt aatggcccct ccaacaacaa tacctgccac acctaagtca ttaattttct
    39241 tggcttcttc tggggaatga atctttcctt cggcaatgac agcaatgcct gctttgcaaa
    39301 gagcttctat caatgccaca tcaggacctg cttcctgacg gctatacggg gtgtagcctg
    39361 acaaggtggt tccaacaaaa tcaatgccag cctgatgggc cactagtcct tcatcaaagg
    39421 tactaatatc tgccatcaaa agctgattgg ggtacttttc cttaacttgt cgaataaagc
    39481 tagcaatatc caagccatca tgtcggtcac gcttggtaca atccatagca atcactgcaa
    39541 tatttaaggc agctaattga tccacttcgg ccatcgttgc cgtaataaaa ggctcttgcg
    39601 gcgggtaatc ttttttgatg ataccgataa ttggtaggtc tgttatggct tgaatttctt
    39661 taatgtctct aaccgaatta gctctgatgc cgacagcacc agcttcttga gcagcttttg
    39721 ccagcagtgg cataataccc ccagtctctg aatagagagg ctcacctggc aaagcctgac
    39781 aagaaacgat aatcccaccc tttaactgct ccatgagctt ttctttggtt ggtttatcag
    39841 gcatagtttc tactccttgt gtttcttagt tttaataaaa ataacgttca caaagttatt
    39901 ataacaaaaa ataaagcgct ttcaatagca atgagacttt tagaatctac tttcattttt
    39961 tatattattt tctattattt ggaatgctct ttcatacaac aaataaaaag gtaaggagtt
    40021 gacaccatac ttatacattg caaatttccg attagaaaac gttaaaaaac caagagcctc
    40081 gagcactctt ggtttacttt agttgtattt tttgcaatta agctgataat actttacgtc
    40141 ctttacgacg acgtgcagca agcacgcgac gtccgttttt agttgacata cggtgacgga
    40201 atccgtgttt acgttgacga cggattttac ttggttgata agtacgtttc acagtgaata
    40261 cctcctcata gattttgtat tcgtttagcc ggctatttgt gatatcagtt actaaacata
    40321 ctataatatt ctatcttaaa gagggtctac tgtcaagatt tattttcgtt tttcaacagt
    40381 ttctctctca tttaagaggc cgctcatttc ataaagtgtc tcttctttga cactgttcct
    40441 ttgccttacc gttgaaacct tatcaatagc aacaataatc aatgagatca gtaaaacaaa
    40501 cggttaagct ggccatacaa aaaatcttcg atagctaaac taactaccaa agattctttt
    40561 aggatctaac gttgaagact gactaccaca tagcggttag ggtcattgcc ttcagaataa
    40621 ctatcaaccc cttcaatgga tgaaacggtt ttatgaacga ttttacgctc actgttactc
    40681 atgggatcca tggtgtaatc ttggccagat tccaagacgc gtttggcaac tttttgcgta
    40741 aagtcaatca aggtttctgt tcgatgttcc acataatcat ggacattcaa ggatactgaa
    40801 aaattttttg aatagcggtc gtgcaagaag ttttgcgcca acaactgcaa ggatttcaaa
    40861 accttaccat ggtaaccaat cacacgtccc gcctctggcg tttcaatctg caaattgatt
    40921 tggcgacgat tgttgctggt ttcaacggtg gcttcgatat ccatttcata gatgatttta
    40981 gtcacataat cggacacttc ctgagcagct ttttcaatat ctttgctgaa accatcatta
    41041 ccaggatctt ggggcacttc tgattcctcg actgggattg acggttgttg tacttttctt
    41101 tcagtgactg tctcagcaag attagaaggg gtttgcgctg gactatcttc agacatgaga
    41161 ggaaccactt tggtatcttc tgcttctaac ttttgagtgg cttttatttc ttcaggttct
    41221 acatcagcag aattgaccgc gggagcattt ttttgattaa tatcctctgg aacacctcga
    41281 gtggcttttt tatcagcctt ataaaccgtc ttgtcgctga taccttcgat atcaacttgg
    41341 gctggctttt taccgaaccc gaggaaacct tttttctctt ttgaaatgac tttgatatgc
    41401 gctttaaggc gtgataaccc taactcttga agtcctgttt cgatagcttc ttcgactgtt
    41461 tttcctgtaa ataataccat aacctaaact cctattttct cttcattgct tttttacgag
    41521 cacgacgttc acgaagacgg cgttcttttt cttcattggc aagacgctgt ctttcggcaa
    41581 taatcttaaa aggattgttt aataacagta gttgtaccac ttgaaaggca tttgagaccg
    41641 tccaatagag cactactcca ctagccaagt taaagcccat gaaaaagatc attagcggca
    41701 tcacataaat cataacagtc atcatgacat ttttttcttt agccgctaga ttggtcaacc
    41761 aagtggacaa gaaagtgaag acagctgcca aaactggtaa aaggtacaaa tgatcatgtt
    41821 gtgccaattc cacccacaag aaagtccctg tttttaagaa agatacccgt gtcaaggctt
    41881 ggaataaggc aatcataact ggcatctgaa ttaagagggg taagagactg gcataagggt
    41941 taaccccata ttttttgtaa agggcttgac tttcttcagc caacttcatc cgcgtttgtg
    42001 tgtctttacc agcgtatttc ttttgcaatt ctctaagctc aggttggatg tcttgcatct
    42061 tctggcttga tttgatttgc atattaaaca aaggcatgag catgagacgg atagtaaggg
    42121 taaaaagaat aatgccaaca ccaattgaac catcaaagga aagccattga attgctctgg
    42181 caaataagta aaccaactgg tcccaaccac tagatgattg tgccgttacc tcaccacgac
    42241 cacaggccac taataataga actaagggga ctattcttgc aattttaatg tttttcttca
    42301 ctctcaaaac ctttctcaag caattgtgct aactttaaaa catgatgtaa attttgttga
    42361 agctcttgat actccaaaga ctcgacaccc ttacgggcaa tcaccacgaa atcctctgac
    42421 ttcagctgat accctaatgc catgataaca tgacgtatct ttcgtttgac tgcatttctg
    42481 gtgactgcat ttcctatttt tttaccgaca gaaataccca cacggaaatg gtcttggcct
    42541 ctatttaaat gataaatgac aaattttcga tttgctgtac tttttccatc cttaaatatg
    42601 gcttggaaat ctttctcaca cttgacacga taggtcttct tcaaaattta actccataat
    42661 ctaaattatt accattatac cacatcccct aaaaaattaa aaagccctaa ccctttcaga
    42721 ttaagcctct ttctgtacta tttctagttt ttgtgcttca actgcctctt gtcactccat
    42781 cttttcgtag tatagttctt ctttcgacaa aggaataacc ttcagaaaac aaccataaaa
    42841 agtcagaatc cacaagacgt cagctactta tccctgtatt tacctatcaa aaagcccggc
    42901 aagccgggct ttaatactga ttttacaatc agctaatgtg atatccaaac aaagacaact
    42961 gacatcgtca ttctgtctgc cgagccatca tatcaaaaat actatttgtt tatggagaga
    43021 gagaaaacaa tattttgatt gaattgggga ttgattgctc gataaactca tgacttcgta
    43081 aatctttatt tacattaatt attatagcga aaaatacttc aataagagca tcatttcctg
    43141 aaatgttgct ttttcgtcct aaaatgttat tgctctctta actttagcga agagcctccc
    43201 accgttcaaa cagacatttc atttttaatc gtgataccat acaagaatca ccattttcga
    43261 aataaaggag ctgcttactc ttatcaattc gaacaacctt atctagattc acaaggtagg
    43321 atctatggca caaaaaaagt ttcggtgcta ctgcttgaat ctcagataga ttaccataaa
    43381 actctaatct ttctgtctga gtccataaac aaactttgtg aggagttgtg gctgtagcaa
    43441 agtataaaat atccttataa ggcaatttta atcttgtctg tggagtctca aataaaaaca
    43501 tgtcctttga ctcacgacta gacatcatat cgtaagtata tcggatacat tcttcgattt
    43561 gttctttaaa ctgtttttga ccacctgctt tatcaataaa atctagagca gataccttat
    43621 atttaaaaga aataggagca aattctgaat gagtggtgac aaacacaata acggcattag
    43681 gatctttttg tcgaatagct gcagctaatt caagcccaca acgagtatat tcaccaattt
    43741 caatatctaa aaaatacagc tgatggtctc ctctttcttg gatactctca aataattttt
    43801 gaggagtcga gaacacttca agctgattac atggtattct agtctctttt aagataccta
    43861 ctacgataga ttctatgcgt gtctgctgga taaaatcgtc ctcaagaata aaaatgttca
    43921 tcatacttta tctcatttct aacaattgtc ttaatcgata atggtcactt ttagtcgaaa
    43981 gcgttatttg ggggtaacta tctaaaacag tcaaaaagtg ggctgagttc tgctgctgag
    44041 catcaaagcg tttggctata tccacacgat tggtttttgt gctattttca ataataaata
    44101 attgcttgtt atcttgttta aagtatgcaa tagataaaaa aggacgtcta ctgcctttgg
    44161 ctgtctcaat agcattgtcc actagtccag taaaaagtac aattaaatcg attaacttaa
    44221 taggtaaatg atcgataaca tctggaatct caatataaag ttcaataccc aaatactgag
    44281 cttcaagcaa tttggaattc atgatgctgc gaatcacagc ctctttgata ttgtctaaag
    44341 aagatatttc ataatttgtg cgcatcatag aagttgccga ttgctgaata accgtgtcat
    44401 aaactctagt aatcagatct atatcaccac tatcgatact atcttttaaa ctaattaaaa
    44461 tattttcaga atcatgttta actgttctga tttctcggta aagttgctca atcgattgat
    44521 tgtaattttc aagatacgca atacgttcat tttgtgcttg tgttaatttt tgtgacagct
    44581 gatctttagc aaatcgatct aatttcacaa taacccaaat aaaaatagct aaataaataa
    44641 atactaaaat agaccggtac tggaaatata gtgcaagaaa tccgctttgc acagttacca
    44701 aaaaatgggc gaaaaagtag taagaaaaca tggcagtatt catccaaaat acccactttt
    44761 tcatcttatc ttcactgata aaccttatta agcttaagtc aatactgaaa atgtagctaa
    44821 acagataaaa tattggtaat acaaaaagat aacataactc taaaagctta aggttattat
    44881 taatttgacc aatgctaaat cctaagacag ctggtaaaaa gaaagatccg atgacgcgaa
    44941 acagtaattc gacaatcaag atagtaaaga aaccattaaa catatgctca aataatttct
    45001 ttttcggagc acttaacaaa ctaacaataa taaaaaacag ttggtcaatc aaaatgactt
    45061 tgtcaaatat catattagca attacaaaac taatgcctat aacgctaacc cgtttccagc
    45121 ttagtttaat agcagaaacc tttgcaaaaa tgacccagct gttaataaag atagtagcat
    45181 agaataaagc catactgtat ataaccatca tctatccacc tcctcctttg gcatggtcaa
    45241 ggtttggcta aattccttat caaaactttt agtccgcaag gataaataag gatacttttt
    45301 gagaatacgc tgcgcattag aaaggccgat accacgttct gaaccttttg aagaatagcc
    45361 ttcttcaaag atcttgttaa tgtctacctt ttttttagtc gtatttgtca caacaaacat
    45421 ctgatagtca tctaacaaaa agtaagcaat tgacatatga ggttgctgtg cttcaagagc
    45481 cgcctcaatg gcgttatcac aaaaaataga catcaacaat agtaagtcca actcaggaat
    45541 gtaagtctct tttatattat ctggcacttc cacattcaaa tcaatacctg atttttccgc
    45601 actgataatt ttagctgaca ataagctctt aatcgatgag atattaattt tcctaagctt
    45661 cgagatatta tagtgtttat catcccagta gtcgttagct tcatgaaccg tttgggcata
    45721 aatatcttga atctgagtaa tggattcact cttaatggct tgacctaaac gctctatacg
    45781 gctcaggtaa tcactttgaa aagctcttac atctcgatag agtttctcaa gatgtttccc
    45841 ataagtctct aagttagtca tataagcttg ttgttctctt ctcaactcat ttttaaggta
    45901 ttctttagaa ctatgattaa aataagatag aagataaact aaaagaagta taaacatcaa
    45961 aaacaataac ttataaggtg tgggaacaaa aagagaatga tgaaaatgta aaaaggggtc
    46021 cgtaaatcct aaaatgacaa aaatgtccac ataataaacc aaaacaaaaa tcagcaatgt
    46081 ttgatagaaa ttatgcgatc gtttcgaata tccggattgg catatcatct tgtaatcttt
    46141 tccaatcata taattaataa tagcaaaact tggaaaaatc agcagataag caagcaagtc
    46201 aaagataata tgacccagat aaataccact cgacaaaaaa agatagggga taacgattgt
    46261 acccataaat cgagatagca aatccaccaa aacactaggt gcaacagcca aaaaaagagc
    46321 tttcaaagaa aataattgct tggtttcttg ccttaaaaca actaaaaata atagaggatc
    46381 aattaacaat ggcgacaaat acgggaactg attgaaaaat agggctaaag ccaaaatcgt
    46441 tagttgtttc caaactggta atgtgatatc agatagaaag gaaaaaatat aaatttttgt
    46501 catataatag acaataattg ataataataa aaacatatat tcgctattag ccattttttt
    46561 ctcctttcca actttattat tttagcatat t
//
LOCUS       DAUDYX010000008        44028 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.008, whole genome shotgun sequence.
ACCESSION   DAUDYX010000008 DAUDYX010000000
VERSION     DAUDYX010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44028
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.008"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            30..563
                     /locus_tag="VHD02_000441"
     CDS             30..563
                     /locus_tag="VHD02_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEQ5497074.1"
                     /translation="MLAISEIRKHPDGLSFDRLCDVKSMLLERDQQIIDIKAVKAVGN
                     VRYDKGLYLLDYQLSYEVILPSSRSMVPVCLSEVQHIQELFIEATDLADKKELVEDNL
                     VLVLDKDAINLEESIVDNILLAIPLQVLTEEEKKSKELPAGQNWAVLTEEDYQCLKEE
                     KQKENNPFASLQGLFDE"
     gene            830..1516
                     /gene="covR"
                     /locus_tag="VHD02_000442"
                     /gene_synonym="csrR"
     CDS             830..1516
                     /gene="covR"
                     /locus_tag="VHD02_000442"
                     /gene_synonym="csrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CovR"
                     /protein_id="HEQ5497075.1"
                     /translation="MTKKILIIEDEKNLARFVSLELQHEGYEVIVEVNGREGLETALE
                     KEFDLILLDLMLPEMDGFEVTRRLQTEKTTYIMMMTARDSIMDVVAGLDRGADDYIVK
                     PFAIEELLARIRAIFRRQDIESEKKVPSQGIYRDLVLNPQNRSVNRGDDEISLTKREY
                     DLLNILMTNMNRVMTREELLSNVWKYDEAVETNVVDVYIRYLRGKIDIPGKESYIQTV
                     RGMGYVIREK"
     gene            1522..3024
                     /gene="covS"
                     /locus_tag="VHD02_000443"
                     /gene_synonym="csrS"
     CDS             1522..3024
                     /gene="covS"
                     /locus_tag="VHD02_000443"
                     /gene_synonym="csrS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CovS"
                     /protein_id="HEQ5497076.1"
                     /translation="MENQKQKQKKYKNSLPKRLSNIFFVLFFCIFSAFTLIAYSSTNY
                     FLLKKEKQSVFQAVNIVRVRLSEVDSNFTLENLAEVLYKNDKTHLRIDDRKGSRVIRS
                     ERDITNTLDANQDIYVYNIDKQMIFTTDNEESSPGLHGPIGRVYHDHIEDQYRGFSMT
                     QKVYSNRTGKFVGYVQVFHDLGNYYVIRARLLFWLLVVELFGTSLAYLIILITTRRFL
                     KPLHNLHEVMRNISENPNNLNLRSDISSGDEIEELSVIFDNMLDKLETHTKLQSRFIS
                     DVSHELRTPVAIIKGHIGLLQRWGKDDSDILEESLTATAHEADRMAIMINDMLDMIRV
                     QGSFEGHQNDMTVLEDSIETVVGNFRVLREDFIFTWQSENPKTIARIYKNHFEQALMI
                     LIDNAVKYSRKEKKIAINLSVTGKQEAIVRVQDKGEGISKEDIEHIFERFYRTDKSRN
                     RTSTQAGLGIGLSILKQIVDGYHLQMKVESELNEGSVFILHIPLAQSKES"
     gene            3239..3733
                     /gene="nrdR"
                     /locus_tag="VHD02_000444"
     CDS             3239..3733
                     /gene="nrdR"
                     /locus_tag="VHD02_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEQ5497077.1"
                     /translation="MRCPKCNYHKSSVVDSRQAEDGNTIRRRRECEQCHTRFTTFERV
                     EELPLLVIKKDGTREQFSRDKILNGVVQSAQKRPVSSTDIENVISRIEQEVRTTYENE
                     VSSTAIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITNRVRGKKKRLNN
                     DETN"
     gene            3717..4892
                     /locus_tag="VHD02_000445"
     CDS             3717..4892
                     /locus_tag="VHD02_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEQ5497078.1"
                     /translation="MMKPIDTFTYLKRNKVTLDSVTLIQLYFPIIGSDAVSIYQYFIH
                     FFDDGLQRHKFSDILNHLQFGMKRFEDALAILTAMELVSVYQLSDTYLITLHQPLSRD
                     LFFQHPAYSRLLEQKIGEVAVSELQVTVPSQARNISKRFSDIFGVQGDLTNVPQKPQK
                     NFDLSSFQQLMVRDGLQFEDNQKDIISLYSIAEQYDMTWFDTYQIAKATAVNGKIRPE
                     RLLAKKNQSMTKPSKENFSQAEQIILREAKQDSALVFLEKIKKARRATITKDERILLQ
                     TLAKMNFLDDVINVMVLYTFNKTKSANLQKSYVLKMANDFAYQKVSTAEEAIVVLRAF
                     TDRQSRRQSKVKTSQSNVPKWSNPDYQETTSQEEQAKLDQFKQAALKRLENLGKGGD"
     gene            4893..5795
                     /gene="dnaI"
                     /locus_tag="VHD02_000446"
     CDS             4893..5795
                     /gene="dnaI"
                     /locus_tag="VHD02_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEQ5497079.1"
                     /translation="MEKIGETMAKLGQNTRVNSDQLIQTILADPEVASFISQHHLSQE
                     QINLSLSKFNQFLVERQKYQLKDPSYIAKGYQPILAMNEGYADVSYLETKELVEAQKQ
                     AAISERIQLVSLPKSYRHIHLSDIDVNNASRMEAFSAILDFVEQYPSAEQKGLYLYGD
                     MGIGKSYLLAAMAHELSEKKGVSTTLLHFPSFAIDVKNAISNGSVKEEIDAVKNVPVL
                     ILDDIGAEQATSWVRDEVLQVILQYRMLEELPTFFTSNYSFADLERKWATIKGSDETW
                     QAKRVMERVRYLAREFHLEGANRR"
     gene            5858..7168
                     /gene="der"
                     /locus_tag="VHD02_000447"
     CDS             5858..7168
                     /gene="der"
                     /locus_tag="VHD02_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEQ5497080.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRQFSLIDTGGIDDVDAPFMEQIKHQAQIAMEEADVIVFVVSGKEGVTDADEY
                     VSKILYRTNTPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPVSSVHGIGTGDVLDAIV
                     ENLPVEEAEENDDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DADGQEFTMIDTAGMRKSGKIYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTIDKDNHTVAKWEADIRDQFQFLTYAPIIFVSALTKQ
                     RLNKLPDLIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVSVKP
                     PTFVVFVNEEELMHFSYLRFLENQIRAAFTFEGTPIHLIARKRK"
     gene            7375..10473
                     /locus_tag="VHD02_000448"
     CDS             7375..10473
                     /locus_tag="VHD02_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ5497081.1"
                     /translation="MMARLIPGRVRNEGIKLYEQGLVSFQDDNKGILQIEVETYQVQY
                     GADDEDITCQCDTFHMKHYCKHIAAVEYFLKNDQKGKLFLKQLTNQTKIKETTKKMTS
                     FGSLFLDSLAMNEDDSVKYRLSALGSRSPFSSDYWWSLKINRLPDDRSYVIRDIKGFL
                     QLIKKEGFYQIGKNYFEQLSWLQFDPSSQDLIEFLWRLASNTDKGDNENIFPNHARHL
                     RLPSGFFEEGIHYLTSLYDFTFEGPSQTYHHLFVRSLEAEAGLYEFKVEVYRKSIELQ
                     IAEKNVQYLFDNDYLLYQDTFYHLTLKQRKMVQAIRSLPIEADLAKHIHFDLDDHAKL
                     AASLSDFKQIGLVEAPKSFAIRDFEVTFQFDLLNRDEISCQLMFDYGNYQVSDKASLE
                     ALPFASHLKKEEKINRSLLAFGFSPQFYSKKRLTSAKELYTFFEETVPCFERLGNVAL
                     STAIQALQVKEMPKIAIRRNQGLLDISFDFSTIIENDIDQAVTALFQNNPYFVSQTGQ
                     LVVFDDETQKVSKSLQELRARQLKNGHLQLDGIRALQVSKLFEGMTSVHFSKELEELA
                     YHLQHPETFSIKPLPVKAQMRDYQRNGVQWLSMLNHYGFGGILADDMGLGKTLQTLAF
                     LASHLKSDSKVLILSPSSLIYNWFDECQKFTPQLDVVVSYGLKQIRDQIIEEGHQITI
                     TSYSSFRQDFETYQAFHYDYLILDEAQVIKNAQTKISHCLRAFNTANCFALSGTPIEN
                     KMLEIWSIFQIVLPGLLPTKKEFLKLTAEQVSRYIKPFVMRRKKEDVLPELPDLIETN
                     YSNEMTDEQKAIYLAQLRQMKDQIRNSSDVDISRQKIEILSGITRLRQICDTPSLFMD
                     YQGKSGKLDSLRILLTQIKENGHRALIFSQFRGMLDLAKQEMTALGLTSYQMTGSTPA
                     NERQEMTRAFNNGSKDAFLISLKAGGVGINLPGADTVILIDLWWNPAVEMQAISRAYR
                     IGQKENVEVYRLITRGTIEEKILELQESKRNLVTTVLDGNESRASMSIEEIKEILGLN
                     TH"
     gene            10716..11318
                     /locus_tag="VHD02_000449"
     CDS             10716..11318
                     /locus_tag="VHD02_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497082.1"
                     /translation="MVKNQFPLVADGIAISDPAKQMALYENEDLITNIRGYYQDKEYD
                     DIARNEEFTAKATSRQAPSSKRFCSNDEKHHYVKEARQKAKQDLKEKRQAYLAKEMAY
                     VPKQVSKKQQPADSSPSQKQATTEMSRFTKKLHQDNYILAELPKEYKEPKNLPQQGTT
                     KKNNYDFLKSSQIYNNKEMRQQREKTIAQELNLSRFEDLP"
     gene            11358..12686
                     /locus_tag="VHD02_000450"
     CDS             11358..12686
                     /locus_tag="VHD02_000450"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEQ5497083.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFSEDNITPDMELIVGNAFRENNKEVAYALRHQIPFKRYHDFLGDFMKSF
                     ISFAVAGAHGKTSTTGLLSHVLKNITDTSYLIGDGTGRGSANAQYFVFESDEYERHFM
                     PYHPEYSIITNIDFDHPDYFTGIADVRNAFNDYAKQVKKALFVYGEDDELKKIEAPAP
                     IYYYGFEEGNDFIAYDITRTTNGSDFKVKHQGEVIGQFHVPAYGKHNILNATAVIANL
                     FVAGIDMALVADHLKTFSGVKRRFTEKIINDTIIIDDFAHHPTEIVATIDAARQKYPS
                     KEIVAIFQPHTFTRTIALLEDFACALNEADSVYLAQIYGSAREVDKGEVKVEDLAAKI
                     IKPSQVVTVENVSPLLDHDNAVYVFMGAGDIQLYEHSFEELLANLTKNNQ"
     gene            12732..13214
                     /locus_tag="VHD02_000451"
     CDS             12732..13214
                     /locus_tag="VHD02_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ5497084.1"
                     /translation="MLIRQVQGSDLEVIATIESDNFSPQEATTRAVLEERIRLIPDTF
                     LVALIDQEIVGYIEGPVVTTPILEDSLFHGVTKNPKTGGYIAITSLSIAKHFQQQGVG
                     TALLAALKDLVVAQQRTGLILTCHDYLISYYEMNGFINQGISESQHGGTLWYQMIWKL
                     "
     gene            13326..14900
                     /gene="mltG"
                     /locus_tag="VHD02_000452"
     CDS             13326..14900
                     /gene="mltG"
                     /locus_tag="VHD02_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEQ5497085.1"
                     /translation="MALTDFKDKDQQDQQRSFKEQILAELEKANQIRKEKEEELFQKE
                     LEAKEAAKRTAQLYAEYKRQDAFQKESIAHNNKTAKHFQAIKGAVMTSEALKPTLLSE
                     KENSSLKTTNKRVVQANELQETASKESQVPLTIEKGHSVRRKLSKRQQTERAAKKIST
                     VLISSIIITLLAVTLAGAGYVYSALNPVDKNSDAFVQVEIPSGSGNKLIGQILQKKGL
                     IKNSTVFSFYTKFKNFTNFQSGYYNLQKSMSLEEIASALQEGGTAEPTKPSLGKILIP
                     EGYTIKQIAKAVEHNSKGKTKKAKTPFNEKDFLDLVTDEAFIQDMVKRYPKLLATIPT
                     KEKAIYRLEGYLFPATYNYYKETTMRELVEDMLAAMDATLVPYYDKIAASGKTVNEVL
                     TLASLVEKEGSTDDDRRQIASVFYNRLNSGMALQSNIAILYAMGKLGEKTTLAEDATI
                     DTTINSPYNIYTNTGLMPGPVASSGVSAIEATLNPASTDYLYFVANVHTGEVYYAKTF
                     EEHSANVEKYVNSQIQ"
     gene            14973..15455
                     /gene="greA"
                     /locus_tag="VHD02_000453"
     CDS             14973..15455
                     /gene="greA"
                     /locus_tag="VHD02_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEQ5497086.1"
                     /translation="MAEKTYPMTLTEKEQLEKELEELKLVRRPEIVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQISTLETKIRYAEIIDSDAVAKDEVAIGKTVIVQEVGTTD
                     KDTYHIVGAAGADIFSGKISNESPIAQALIGKKTGDKVRIESPAATYDVEIISVEKTN
                     "
     gene            complement(16079..17002)
                     /gene="yidC"
                     /locus_tag="VHD02_000454"
     CDS             complement(16079..17002)
                     /gene="yidC"
                     /locus_tag="VHD02_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEQ5497087.1"
                     /translation="MKLTLNRILFSGLALSILFTLTGCVGRDAHGNPKGMIWEFLGKP
                     MSYFIDYFANNAGLGYGLAIIIVTIIVRTLILPLGLYQSWKASYQSEKMTFLKPVFEP
                     INKRIKQASSQEEKMAAQTELMAAQRAHGINPLGGIGCLPLLIQMPFFSAMYFAAQYT
                     KGVSTSTFMGIDLGSRSLVLTAIIAALYFFQSWLSMMAVSEEQREQMKTMMYTMPIMM
                     IFMSFSLPAGVGLYWLVGGFFSIIQQLITTYLLKPRLHKQIKEEYAKTPPKAYQSTSS
                     RKDVTPSQNMEQANLPKKIKSNRNAGKQRKR"
     gene            complement(17084..17362)
                     /locus_tag="VHD02_000455"
     CDS             complement(17084..17362)
                     /locus_tag="VHD02_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEQ5497088.1"
                     /translation="MQKVRLIVSGRVQGVGFRYATHTLALDIGDIYGRVWNNNDGTVE
                     ILAQSKDSDKIATFIQEVRKGPSKWAKVTYVDVTMANFEDFQDFQIGN"
     gene            17623..18360
                     /locus_tag="VHD02_000456"
     CDS             17623..18360
                     /locus_tag="VHD02_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEQ5497089.1"
                     /translation="MVITSKANQLIKQTKKLLQKKHRKQSYLIEGWHLFEEAQKSGQV
                     FRHIFVLEEMVERLAGEQELVIVSPQVLKELTDSPSPQGIVAEVEIPKLAFPSDYKGK
                     YLVLEDVQDPGNLGTIIRTADAARFDGVFLSEKSADIYNQKTLRSMQGSHFHLPIWRT
                     DVYQLCRELQEYETPILATTLSKKSVDYKSLTHHERLALVLGNEGQGISAEMAALADQ
                     LVHITMPGQAESLNVAVAAGILIFSLI"
     gene            18399..18899
                     /locus_tag="VHD02_000457"
     CDS             18399..18899
                     /locus_tag="VHD02_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEQ5497090.1"
                     /translation="MHYTEDKEYMEHVGHLIAHPRFQKLSHIVQHQHSTRLEHSINVS
                     YSSYKLAKRFGWDAKSTARGGLLHDFFYYDWRVTKFNKGHAWVHPRIAVRNAKKLTEL
                     NKKEEDIILKHMWGATIAFPRYKESYIVTMVDKYWAVKEAVTPLRQKWSNRRFLHRKT
                     LQSHNR"
     gene            18914..19603
                     /locus_tag="VHD02_000458"
     CDS             18914..19603
                     /locus_tag="VHD02_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEQ5497091.1"
                     /translation="MNDHVIYTQSDVGLNQFFAKIYSLVGMGVGLSAFVSYLMLYPFR
                     ENLISILVNQPMIYYGAAIIELILVFVASGAARKNTPAALPIFLIYSALNGFTLSFII
                     VAYAQTTVFQAFLSSAAVFFAMSIIGVKTKRDMSGLRKAMFAALIGVVVASLINLFIG
                     SGMMSYVISVISVLIFSGLIASDNQMIKRVYQATNGQVGDGWAVAMALSLYLDFINLF
                     ISLLRIFGRND"
     gene            19781..20023
                     /locus_tag="VHD02_000459"
     CDS             19781..20023
                     /locus_tag="VHD02_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEQ5497092.1"
                     /translation="MSTAIWILLLIVALGVGVFGGIFIARKQIEKEIGEHPRLTPEAI
                     REMMSQMGQKPSEAKIQQTYRNIIKQSKAAVSKGKK"
     gene            20201..20995
                     /gene="racE"
                     /locus_tag="VHD02_000460"
     CDS             20201..20995
                     /gene="racE"
                     /locus_tag="VHD02_000460"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEQ5497093.1"
                     /translation="MDTRPIGFLDSGVGGLTVVCELIRQLPHEKIVYIGDSARAPYGP
                     RPKKQIKEYTWELVNFLLTQNVKMIVFACNTATAVAWEEVKAALDIPVLGVVLPGASA
                     AIKSTTKGQVGVIGTPMTVASDIYRKKIQLLAPSIQVRSLACPKFVPIVESNEMCSSI
                     AKKIVYDSLAPLVGKIDTLVLGCTHYPLLRPIIQNVMGPSVKLIDSGAECVRDISVLL
                     NYFDINGNYHQKAVKHRFFTTANPEIFQEIASIWLKQKINVEHVTL"
     gene            20992..21978
                     /locus_tag="VHD02_000461"
     CDS             20992..21978
                     /locus_tag="VHD02_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEQ5497094.1"
                     /translation="MSEKIYEYKDENNWFIGKMTGHNLISGWGVKHTTIKKIDDLLDG
                     IAATLDWENPKGYDVSVVRYQSPLSLITFIIDMINQETQREIKVIPHAGTILLMENAK
                     LLAVYLPEGGVSTATFFATSEQGFGDTILIATRNEGKTKEFRNLFGQLGYRVENLNDY
                     PELPEVAETGTTFEENARLKAETISRLTGKMVLADDSGLKVDALGGLPGVWSARFSGP
                     DATDAKNNAKLLHELAMVFDQKKRSAQFHTTLVVAAPNKDSLVVEAEWPGYIATQPKG
                     ENGFGYDPVFIVGETGRHAAELEADQKNQLSHRGQAVRKLMEVFPAWQAKQS"
     gene            21957..22478
                     /locus_tag="VHD02_000462"
     CDS             21957..22478
                     /locus_tag="VHD02_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEQ5497095.1"
                     /translation="MASKTIIVMSDSHGDRDIVQAIKDKYLGQVDAIFHNGDSELNSS
                     DPIWAGIYVVGGNCDYDTGYPDRLVTQLGTVTIAQTHGHLYHINFTWDKLDYFAQEVA
                     ADICLYGHLHRPAAWQVGQTLFMNPGSVTQPRGEINEKLYARVELTDTQIKVDYFTRD
                     HKLYPSLSKEFKR"
     gene            22475..22936
                     /locus_tag="VHD02_000463"
     CDS             22475..22936
                     /locus_tag="VHD02_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEQ5497096.1"
                     /translation="MIAKEFETFLMSHLDNYLIPEQDLAIFIDTHNADHVMLLLVSNG
                     FSRVPVITREKKYVGTISISDIMMYQSKRQLTDWEMSQTDIGEMVNTKIETISITSSL
                     TEIMHKLIDFPFLPVVDRANRFVGIITRKSILKAVNSLLHDFTDDYTIIKK"
     gene            22933..23679
                     /gene="xerD"
                     /locus_tag="VHD02_000464"
     CDS             22933..23679
                     /gene="xerD"
                     /locus_tag="VHD02_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEQ5497097.1"
                     /translation="MKSYIEPFIASKALSQNSQKAYRYDLQQFCQLVGERVNQDKLLL
                     YQNSIANLSLSAKKRKLSTANQFLYYLYQIKYLNSYFRLTDTMKVMRTEKQQAAIINT
                     DIFYQKTPFVWGQLISLLILELGLTPSEVAGIEVANLDLNFQMLTLKTKKGVRVLPLS
                     QILIPFLEQQLVGKEVYLFEHRGIPFSRQWFFNHLKTFVRSIGYEGLTAQKLREQFIL
                     KEKLAGKSIIELSDILGLKSPVTLEKYYKS"
     gene            23679..24383
                     /locus_tag="VHD02_000465"
     CDS             23679..24383
                     /locus_tag="VHD02_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEQ5497098.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSQYKVDIYEVPIVEVIEQYLNYIET
                     LQVMKLEVAGDYMLMASQLMLIKSRRLLPKVVEHIEEEDLEQDLLEKIEEYSRFKAVS
                     QALAKQHDQRAKWYSKPKQELIFEDAILQEDKTVMDLFLAFSNIMAAKRAVLKTNHTV
                     IERDDYKIEDMMASIKQRLEKENVISLSAIFEECQTLNEVISIFLASLELIKLHVVFV
                     EQLSNFGAIILRKEKK"
     gene            24380..24931
                     /locus_tag="VHD02_000466"
     CDS             24380..24931
                     /locus_tag="VHD02_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEQ5497099.1"
                     /translation="MTYLSQIEALLFVAGEEGLSLRHLASMLSLTPTALQQQLEKLSQ
                     KYEKDQHSSLCLIETANTYRLVTKEGFAGLLRAYAKTPMNQSLSRASLEVLSIVAYKQ
                     PITRIEIDDIRGVNSSGALSKLLAFDLIREAGKKDVVGRPHLYATTDYFLDYMGINHL
                     DELIEVSAVEPADEEIALFRTQD"
     gene            25053..25775
                     /locus_tag="VHD02_000467"
     CDS             25053..25775
                     /locus_tag="VHD02_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEQ5497100.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSG
                     DVVEIEGSPIYNEEKVYYLLNKPRGAISSVSDDKGRKTVLDLLPQVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDTMIHPRNEIDKVYLARVKGIATKENLRPLTRGIVIDGKKTKP
                     ARYNIVRVEADKNRSIVELTIHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRP
                     GEARRLNKKEISQLHNLANTKK"
     gene            25772..26035
                     /gene="yidD"
                     /locus_tag="VHD02_000468"
     CDS             25772..26035
                     /gene="yidD"
                     /locus_tag="VHD02_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEQ5497101.1"
                     /translation="MMKKLLIVSVKAYQKYISPLSPPSCRYKPTCSAYMLTAIEKHGT
                     KGILMGIARILRCHPFVAGGVDPVPEDFSLMRNKNTSKNAEKA"
     gene            26213..26761
                     /locus_tag="VHD02_000469"
     CDS             26213..26761
                     /locus_tag="VHD02_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEQ5497102.1"
                     /translation="MTTKELINKNDKVKKARNHIVLFQPQIPQNTGNIARTCAATNAP
                     LHIIKPMGFPIDDRKMKRAGLDYWDKLELHFYDHLEQFINQCHGQLHLISKFAVNNYS
                     QATYADGDSHYFLFGREDTGLPEDFMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYE
                     ALRQQGFQGLELKHTYEHDKLK"
     regulatory      26870..26994
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            27073..27636
                     /locus_tag="VHD02_000470"
     CDS             27073..27636
                     /locus_tag="VHD02_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ5497103.1"
                     /translation="MSKTHKMIMIGILSAISFLLMLVSFAIIPGAAFLKIEFSIIPVL
                     FGLMIMDLKSAYLILLLRSLLKLFLNNRGVNDFIGLPMNIIAIALFVTAFALVWNRQK
                     TLSQYVFASLLGTGLLTFGMVVLNYTFAIPLYAIFANIDIRAYIGVTKYMMTMVIPFN
                     LVEGLIFAITFYFVYIASKPILERYLH"
     gene            27638..28291
                     /locus_tag="VHD02_000471"
     CDS             27638..28291
                     /locus_tag="VHD02_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEQ5497104.1"
                     /translation="MMTNKQTHFLIASFALLIFVIIGYTVKFFPERLALLDNTIQAEI
                     RGNLPIVLTQFFRGVTVFGNVMTQVLLVIVSVLVLFFMKWKIEALFILSNGAIAAFLI
                     TTLKLFYQRPRPAIEHLVYAGGYSFPSGHAMGSMLIFGSLLIICYQRLHSKLLQFVTS
                     MIFIILILVIGLSRIYLGVHYPSDILAGFVLGFGILQFIYPFYKQKRFEWRFLLKQD"
     gene            28584..29504
                     /locus_tag="VHD02_000472"
     CDS             28584..29504
                     /locus_tag="VHD02_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407327.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEQ5497105.1"
                     /translation="MKKRYQTLNEHYRQLFGAKMFKVPIDAGFDCPNRDGTVAHGGCT
                     FCTVSGSGDAIVAPDAPIKEQFYKEIDFMHRKWPDVNRYLVYFQNFTNTHDTVDVIRD
                     RYEQAINEPGVVGINIGTRPDCLPDDTIAYLAELSERMHVTVELGLQTTYEETSRLIN
                     RAHSYDLYKETVRRLRHYPNINIVSHLINGLPKETHDMMLENVRRCVTDNDIQGIKLH
                     LLHLMTNTRMQRDYHEGRLKLLSQKDYVSIICDQLEIIPKHIVIHRITGDAPRDMLIG
                     PMWSLNKWEVLNAIDKEMERRGSFQGCKVD"
     gene            29543..30097
                     /locus_tag="VHD02_000473"
     CDS             29543..30097
                     /locus_tag="VHD02_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ5497106.1"
                     /translation="MLKRPIHLSHDFLAEVVDKSSVVVDATMGNGNDTAFLAQLAKKV
                     YAFDVQEQAIRKTSERLAQLGLLNAELILAGHEAVDQYVTEPVRAAIFNLGYLPSADK
                     SIITLPNTTLQALSKLLTLLMVGGRIAIMVYYGHDGGSLEKDALLDFVKQLDQRKVSA
                     MLYQPLNQVNTPPFLIMLEKLADV"
     gene            30230..31564
                     /locus_tag="VHD02_000474"
     CDS             30230..31564
                     /locus_tag="VHD02_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEQ5497107.1"
                     /translation="MEDPVSQPLVIQFLLLVVLTLLNAFFSASEMALVSLNRSRVEQK
                     AADGDKKYARLLRVLEEPNHFLSTIQVGITFISLLSGASLSASLGKVISGWLGNSATA
                     RTAGTIISLVFLTYVSIVLGELYPKRIAMNLKDKLAIVSAPIIIGLGRLVSPFVWLLS
                     ASTNLLSRLTPMTFDDADEQMTRDEIEYMLSKSEATLDAEEIEMLQGVFSLDEMMARE
                     VMVPRTDAFMIDINDDPLENIQEILKQSFSRIPVYDVDKDKIIGLIHTKRLLESGFRQ
                     GFDQINMRKMLQEPLFVPETIFVDDLLRQLRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDKAEQFVHEIGDNTYIVVGTMTLNEFNDYFDTELESDDVDTIAGFYLT
                     GIGTIPSQEQKEAYEIDNKDKHLVLINDKVKDGRITKLKLILSNIEQIIEED"
     gene            31642..32433
                     /gene="pflA"
                     /locus_tag="VHD02_000475"
     CDS             31642..32433
                     /gene="pflA"
                     /locus_tag="VHD02_000475"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEQ5497108.1"
                     /translation="MTEKDYEQVTGMVHSTESFGSVDGPGIRFIIFLQGCKLRCQYCH
                     NPDTWEMETNNSKIRTVNDVLKEALQYKHFWGKKGGITVSGGEAMLQIDFITALFIEA
                     KKLGIHTTLDTCGFTYRPTPEYHQVLDNLLAVTDLILLDLKEIDEKQHKIVTRQPNKN
                     ILQFARYLSDKQIPVWIRHVLVPGLTDIDDHLTRLGEFVKTLKNVDKFEVLPYHTMGE
                     FKWRELGIPYQLEGVKPPTKERVQNAKNLMQTESYTEYMNRIHQS"
     gene            32564..33499
                     /locus_tag="VHD02_000476"
     CDS             32564..33499
                     /locus_tag="VHD02_000476"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEQ5497109.1"
                     /translation="MSKILVFGHQNPDTDAIASSYAFDYLSQKAFGLDTEVVALGTPN
                     EETAFALDYFGVEAPRVVESAKAQGSEQVILTDHNEFQQSIADIREVEVYGVVDHHRV
                     ANFETANPLYMRVEPVGSASSIVYRMFKENGIEVPKAIAGMLLSGLISDTLLLKSPTT
                     HVSDHLVAEELAELAEVNLEDYGMALLKAGTNLASKSEVELIGIDAKTFELNGNAVRV
                     AQVNTVDIAEVLERQEAIEAAIKDAMAAEGYSDFVLMITDIVNSNSEILAIGANMDKV
                     EAAFNFTLDNNHAFLAGAVSRKKQVVPQLTESFGA"
     gene            33575..34228
                     /locus_tag="VHD02_000477"
     CDS             33575..34228
                     /locus_tag="VHD02_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEQ5497110.1"
                     /translation="MITIFHSDKLTRQPFFQDLINYLDQHDHVILREIKKAFPNVTGI
                     DKAIESYVQAGYIRRENKRYGINLPLVSSDQQLALDTMLFVDTCSAMYENILAVVFET
                     QLTNQTNHVMIKEKTNITRSQLTLANYFYRLKRGEKPSAEQMDLYDLLGDVNQEYALK
                     YMTTFLLKFTRKDLVMQKRPDIFVEALVTLGYLKQVEPTTYQLLMTLDKESLTFIAP"
     gene            complement(34273..35310)
                     /locus_tag="VHD02_000478"
     CDS             complement(34273..35310)
                     /locus_tag="VHD02_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEQ5497111.1"
                     /translation="MLFMVNKKRRPLLKSHRHLLRLYTSLILLLVSLMGLALSLGESH
                     LSFLDLIHVFLGKSSHAISFIVINIRLPRILAACLGGGSLALSGLLLQRLTRNPLADS
                     GVLGITIGAGISLAIVVSFSFFEQAHISHYLPLFAMLGAIVTTFSVYWLSLTKQGQID
                     PTRLILTGVAVTTILSSLMVALVGHINRYKVDLVINWLSGQLIGDDWPTLSVIAPLLL
                     CFWLLTYSQAHFLNIMGLADNTAIGLGLPLNRKRRLILVLAAGLGALSVLLVGNISFI
                     GLIAGHFSTYLVGSNHKITIPISILIGMILLLVADTVGRVYLVGSNIQTGILVSLIGA
                     PYFLYLMAKTK"
     gene            complement(35271..36323)
                     /locus_tag="VHD02_000479"
     CDS             complement(35271..36323)
                     /locus_tag="VHD02_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEQ5497112.1"
                     /translation="MKTKHPFLCLERFCLKKTQIITKSHIFWLVFVLLSFSLCVAIYC
                     HLRFGAVALSHQDLNSILFGKQNGHKANVLLAIRLPRLFGATLTGSALAVSGTIMQAI
                     TRNPIAEPGLLGINAGAGLALVLAYAFVPHLHYSLIILLSLLGSSLAATLVFGLSYQS
                     GKGYHQLRLVLAGAMVSILLSALGQGITNYYHLTNAVIGWQAGGLVGVNWQMIGYIAP
                     LIILSLCLAQLLSYHLTVLSLSESQAKALGQKTNLISAVFMILVLILSSAAVAIAGSI
                     SFIGLVIPHLMKHFTPHHYRYLLPLCAVSGASFMVWVDIACRNLNPPYETPLGALVSL
                     IGFPCFLWLIRRGGRY"
     gene            complement(36313..37245)
                     /locus_tag="VHD02_000480"
     CDS             complement(36313..37245)
                     /locus_tag="VHD02_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEQ5497113.1"
                     /translation="MKKLTLLLTLCLTTITLIACGNQATNHSNTASKSLSPMPQIAGV
                     TYYGDIPKQPKRVVSLASTYTGYLKKLDMNLVGVTSYDKKNPILAKTVKKAKQVTATD
                     LEAITTLKPDLIVVGSTEENIKQLAEIAPVISIEYRKRDYLQVLSDFGRIFNKEGKAK
                     KWLKDWKTKTAAYEKEVKAVTGDKATFTIMGLYEKDVYLFGKDWGRGGEVIHQAFHYD
                     APEKVKTEVFKQGYLSLSQEVLPDYIGDYVVVAAEDDKTGSALYESKLWQSIPAVKKH
                     HVIKVNANVFYFTDPLSLEYQLESLREAILSSEN"
     gene            complement(37271..38053)
                     /locus_tag="VHD02_000481"
     CDS             complement(37271..38053)
                     /locus_tag="VHD02_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5497114.1"
                     /translation="MTTISAEDLTIAYEQRTIIDKLSFYIPEGKITTIIGANGCGKSS
                     LLKALTRLLPPKQGVVYLNGQNIATLETKEVAKKLALLPQVQEATNGITVYELVSYGR
                     FPHQSYFGNLSPADKKAIHWAMQATNVMAYADQPVDALSGGQRQRVWLAMALAQGTDT
                     IFLDEPTTYLDLNHQLEILELVKSLNKDAGKTIVMVLHDLNLSARYSDHLIAMKHGKI
                     HYTGTIADVMTSPIIQDIFQIKPVLVDDPIHNCPIVLTYQLQ"
     gene            complement(38299..39744)
                     /locus_tag="VHD02_000482"
     CDS             complement(38299..39744)
                     /locus_tag="VHD02_000482"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEQ5497115.1"
                     /translation="MITIEQLLDILKKDHNFREVLDADGYHYHYQGLSFERLSYDSRQ
                     VDGKTLFFAKGATFKADYLKEAITNGLQLYISEVDYELGIPVVLVTDIKKAMSLIAMA
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYHMLKESYKPAMFSTMNTTLDGKTFFKSQLT
                     TPESLDLFAMMAECVTNGMTHLIMEVSSQAYLVDRVYGLTFDVGVFLNISPDHIGPIE
                     HPTFEDYFYHKRLLMENSRAVVINSGMDHFSFLADQVADQEHVFYGPLSDNQITTSQA
                     FSFEAKGQLAGHYDIQLIGHFNQENAMAAGLACLRLGASLADIQKGIAKTRVPGRMEV
                     LTMTNHAKVFVDYAHNGDSLEKLLSVVEEHQTGKLMLILGAPGNKGESRRADFGRVIH
                     QHPNLTVILTADDPNFEDPEDISKEIASHIARPVEIISDREQAIQKAMSLCQGAKDAV
                     IIAGKGADAYQIVKGQQVAYAGDLAIAKHYL"
     gene            39832..41466
                     /locus_tag="VHD02_000483"
     CDS             39832..41466
                     /locus_tag="VHD02_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEQ5497116.1"
                     /translation="MSTEKKQLTQEELMVQGAAWSTAGNFISRLLGVLYIIPWYIWMG
                     QYAIQANALFNMGYNVYAYFLLISTTGLNVAIAKQVAKYNSMGQTEHSYQLIRSTLKL
                     MLGLGLIFSVIMYLGSPLFASLSGGDDTLVPIMHSLSLAVFIFPVMSVIRGIFQGHNN
                     IKPYAISQIAEQLIRVIWMLLTTFLIMKLGSGDYASAVTQSTFAAFIGMVASMGVLGY
                     YLWKQGLLAAIFSKPDHTVSIDIKGLLLETLKESIPFIVTGSAIQAFQLIDQWTFVNT
                     MTLFTDYSRSQLLVLFGYFNANPAKITMVLIAVAASIGGVGIALLTENYVKKDMKAAA
                     RLIINNIEMLVMFLLPALTGAIILARPLYSVFYGASEDRAIHLFVAVLFQTLLMALYT
                     LFSPMLQALFENRKAIYYFAYGILIKLVLQIPLIYLLHAYGPLLATTIALVVPIYLMY
                     RRLYQVTHFNRKLLQKRLLLTLIETLLMGLVVFVANWLLGYAFKPTGRLTSLLYLLII
                     GGVGMTVYTALTLLTHQLDKLIGSKASRLRQKLGWH"
     gene            41634..42263
                     /gene="upp"
                     /locus_tag="VHD02_000484"
     CDS             41634..42263
                     /gene="upp"
                     /locus_tag="VHD02_000484"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEQ5497117.1"
                     /translation="MGKCQVISHPLIQHKLSILRRQTTSTKDFRELVNEIAMLMGYEV
                     SRDLPLEDVDIQTPVSKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRNEETLEPVEYLVKLPEDINQRQIFLVDPMLATGGSAILAVDSLKKRGAANIKFVCL
                     VAAPEGVKKLQEAHPDIDIFTAALDDHLNEHGYIVPGLGDAGDRLFGTK"
     gene            42487..43077
                     /locus_tag="VHD02_000485"
     CDS             42487..43077
                     /locus_tag="VHD02_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEQ5497118.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGTKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRHRLEKILAQNAGK
                     TIKQIHKDAERDYWMSAEETLAYGFIDEIMENNELK"
     gene            43572..43865
                     /locus_tag="VHD02_000486"
     CDS             43572..43865
                     /locus_tag="VHD02_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEQ5497119.1"
                     /translation="MFQKQERIGLVVYLYYNRDARKLSKFGDLYYHSKRSRYLIIYIN
                     KNDLDTKLEEMRRLKCVKDIRPSAFDDIDRQFVGNLHRDETNNHQKGYQPPSY"
BASE COUNT        14065 a         7651 c         9006 g        13306 t
ORIGIN      
        1 ataaatgaga gttttattag gagaagatga tgttagctat ttccgaaatc agaaaacacc
       61 cagacggtct ctcgtttgat cgtttatgtg atgtcaaaag tatgttactt gagcgtgacc
      121 aacagattat tgacataaag gcagttaaag ctgtcggtaa tgttcgctat gataaggggc
      181 tttatctgtt agattatcaa ttgagttacg aagtgatatt accgtctagc cgttctatgg
      241 ttccggtctg tttaagtgaa gtccaacaca ttcaagaatt atttatagag gcgacagatt
      301 tagctgataa gaaagagttg gttgaagaca atctggtatt agttttagac aaagacgcta
      361 ttaacctcga agaaagtatt gtggataata tcttacttgc tattccgcta caggtcttga
      421 cagaggaaga aaagaaatca aaagagttgc cagcaggtca aaattgggca gttttgacag
      481 aagaagacta ccagtgtcta aaagaagaaa agcaaaaaga aaataatcct tttgctagct
      541 tgcaagggtt gtttgatgaa taaaactgaa tattaaagat gtctgtaatt tttatttgaa
      601 aaaacagcta ttctttgata tactctttag agaatatggt tacttatcta aagtagaagg
      661 tattttgacc atagagggca aagaaggtaa tgactattag atgcttcata ttttattcgc
      721 tagaagacta tttgaccatt agaactgaaa tagtctagga tatgagatgg atttcatagt
      781 tttctttctg ggagaaaaag atagattaag aggataaggg ttggtataaa tgacaaagaa
      841 gattttaatt attgaagatg aaaagaatct ggctagattc gtttctcttg agctgcaaca
      901 tgagggttat gaagtcattg ttgaggtcaa tggtcgtgaa gggttagaaa ctgctttgga
      961 aaaagagttt gatttaatcc tgcttgactt aatgttacca gagatggatg gttttgaagt
     1021 gacccgtcgt ttgcaaaccg aaaaaacaac gtatatcatg atgatgactg cgcgtgattc
     1081 tattatggat gtggttgcag gtttagaccg cggtgcagac gactatattg ttaaaccgtt
     1141 tgccattgaa gaactacttg cccgtattcg tgctattttc cgccgtcaag atattgaatc
     1201 tgagaagaaa gtgcctagtc aaggcattta tcgagatcta gttttaaatc cacaaaaccg
     1261 ttcagttaat cgtggcgacg atgagatttc tctcactaaa cgtgaatatg atttgcttaa
     1321 tattttgatg actaatatga atcgtgtcat gacacgtgaa gaattattgt caaatgtttg
     1381 gaaatatgat gaagctgttg agactaatgt tgtagatgtc tatattcgtt atctccgcgg
     1441 caaaattgac attccaggca aggaatctta tatccaaaca gtgcgtggca tgggatacgt
     1501 tattcgtgag aaataagtca tatggaaaat cagaaacaaa aacagaagaa atataaaaac
     1561 tcgttaccaa aacgactatc taatatcttt tttgttcttt ttttctgcat tttctctgcc
     1621 tttacactga ttgcctatag ttcaacaaac tatttcttat tgaagaaaga aaagcagtca
     1681 gtctttcaag ctgtaaatat tgttagagtt cgtctttctg aggtggactc taattttaca
     1741 ttagagaact tagcagaagt tttgtacaaa aacgataaaa cacatctgag aattgatgac
     1801 agaaagggca gtcgagtcat taggagtgag cgcgatatca caaatactct agatgcgaat
     1861 caagatattt atgtctataa cattgataaa cagatgattt ttaccacaga taatgaagaa
     1921 tcatctcctg gcttgcatgg tcctatcggt cgggtgtatc acgaccatat cgaagatcag
     1981 tatcgtgggt tttccatgac acaaaaggta tattctaatc ggactggaaa atttgtgggc
     2041 tatgttcaag tctttcatga tttaggcaat tattatgtca ttagagcaag actgctgttt
     2101 tggctactag ttgttgagtt atttggcaca agcttagcct atttaatcat cttaattact
     2161 acgcggcgct ttttgaagcc tttacacaat ttacatgaag taatgcgtaa tatctctgaa
     2221 aatcctaata atttaaacct gcgctcagat atttcgtcag gagatgaaat cgaagaattg
     2281 tctgttatct ttgacaatat gttagacaaa ttggagacac atactaagtt gcaatcacgt
     2341 tttattagtg atgtcagcca tgaattacga acaccggttg cgattattaa agggcatatt
     2401 ggtctcttac aacgttgggg taaagatgac agtgatattc ttgaagaaag tttgacagca
     2461 acagcgcatg aggctgaccg tatggcaatc atgattaatg atatgttaga catgattcgc
     2521 gtacaaggct catttgaggg acatcaaaat gatatgacag ttttggaaga ttctattgaa
     2581 actgttgttg gtaattttag agttttaaga gaagatttta tctttacatg gcagtcagaa
     2641 aatccaaaaa cgatagcccg tatttataaa aatcattttg agcaggcttt gatgattctt
     2701 attgacaatg ctgtgaagta ttcccgtaaa gaaaagaaaa tcgcgattaa cctttcagtg
     2761 actggcaaac aagaagctat tgttagagtt caagataaag gcgaaggaat ttctaaagaa
     2821 gatattgaac atatctttga acgcttttat agaacggata aatcacgtaa tcgaacaagt
     2881 actcaggctg gattagggat tggcttgtct attctcaagc aaattgtaga tgggtatcat
     2941 ttacagatga aggttgaaag tgaattaaat gaaggttcag tgtttatctt acatattcct
     3001 ttggcccagt ctaaagagag ttagagtagc ttaaggcatt gttaatgtga caggtatagt
     3061 atgtaatcat tagataatag tagcagtctt ttgccacaac tggttagaag ctgatgctat
     3121 ttttttagca atgatggatg acatgaaaga aatactgtca atcgcgatgc cttttgttat
     3181 ccttgagaat atggtaaaat agtgtattag attaatttga tagaattggg gtgtttgcgt
     3241 gcgttgtcca aaatgtaatt accataaatc gagtgttgtt gacagtagac aagctgaaga
     3301 cggcaacacc attcgtcgtc gaagagaatg tgaacagtgt catactcgct ttactacctt
     3361 tgagcgtgtt gaagaactcc ctttattagt catcaaaaaa gatggcacac gagagcaatt
     3421 ttctagagat aaaatattaa atggggttgt ccaaagtgcg caaaaacgac ctgtctcgag
     3481 cacagatatt gaaaatgtca tttctcgtat tgaacaagaa gttcgaacaa cctacgaaaa
     3541 tgaagtgtca agtactgcta ttggtaattt agtgatggat gagttggccg aacttgatga
     3601 aatcacatat gtacgctttg ctagtgtcta caagagcttc aaggacgttg atgaaatcga
     3661 agaacttttg caacaaatta cgaatcgcgt acgcggaaag aaaaaacgtt taaataatga
     3721 tgaaaccaat tgatactttt acatatctaa aacgcaacaa agtaactctt gattcggtga
     3781 ctttaataca gttgtacttt ccaattattg gaagtgacgc agtctctatc tatcaatact
     3841 ttattcactt ttttgacgat ggtttacaac gtcataaatt cagcgatatt ttaaatcacc
     3901 tacagtttgg tatgaaacgt tttgaagatg ctcttgccat attgacagca atggaattag
     3961 tgagtgtgta tcagctatca gatacatatc tcattacgtt gcaccagccg cttagccgtg
     4021 acttgttttt tcaacatcca gcttattctc gattattaga acaaaagatt ggagaggtag
     4081 ctgtctctga gttgcaggta actgtcccaa gtcaagcaag aaatatttct aagcgatttt
     4141 cggatatttt tggggtgcaa ggagatttga cgaacgtacc tcaaaagcca caaaagaatt
     4201 ttgacctttc aagtttccag caattaatgg ttcgtgatgg gttacaattt gaagataatc
     4261 aaaaagatat catcagcctt tacagtattg ctgaacagta tgatatgacg tggtttgata
     4321 cttatcaaat agctaaggcg acggctgtta atgggaaaat tcgacctgaa cgcttattgg
     4381 ctaaaaaaaa tcaaagcatg acaaaaccaa gcaaagaaaa tttttcacag gctgagcaaa
     4441 ttattttgcg agaagcaaag caagacagtg ctttagtctt tttggaaaaa attaaaaagg
     4501 ctagacgtgc aacgattact aaggatgaac gcattttatt gcaaacattg gccaaaatga
     4561 attttcttga tgatgttatc aatgtcatgg tgctttatac ctttaataag acaaaatctg
     4621 ctaatttaca gaagagttat gtcttaaaaa tggccaatga ttttgcctac caaaaggtgt
     4681 caacggctga agaagctata gtggttttgc gagccttcac ggatcgtcaa agtcgtcggc
     4741 aatcaaaagt aaaaacaagt caaagcaatg tgcctaagtg gagcaatcct gattaccaag
     4801 agacaacaag tcaagaagag caggcgaaac tagatcagtt caaacaggct gctttgaaga
     4861 ggttagaaaa cctaggaaaa gggggtgact agatggaaaa gattggagaa accatggcga
     4921 aattgggtca aaacactcgt gttaatagcg accagttgat tcaaaccatt ttagcggatc
     4981 ctgaagtagc aagcttcatc agtcaacatc acttatctca agagcaaatt aaccttagct
     5041 tatctaagtt caatcaattt ttggtggaac gccagaagta tcaactcaaa gatccctctt
     5101 atattgctaa gggctatcag cctattctag ctatgaatga gggctacgct gatgtgtctt
     5161 atttggaaac caaagaattg gtagaagctc aaaaacaggc agctatctct gaacggattc
     5221 aactggttag tttgccaaaa tcctatcgcc atattcattt atcagatatt gatgttaata
     5281 atgcgagccg tatggaagcc ttctctgcta ttttagattt tgtggaacaa tacccatcag
     5341 cagagcaaaa aggcttatat ttgtacggag atatgggaat tggcaagtct tacttgttgg
     5401 ctgctatggc ccatgaattg tctgagaaaa aaggtgtgtc aacgaccctt ctacattttc
     5461 cgagctttgc cattgatgta aaaaatgcca tttcaaacgg ctctgttaaa gaagaaatcg
     5521 atgcggtcaa aaatgtacca gtgctcattt tagatgatat tggtgcagag caagccactt
     5581 cttgggtccg tgatgaggtg ttgcaggtga ttttacaata ccgtatgtta gaggaattgc
     5641 caaccttctt tacctctaat tacagctttg cggatttgga gcgaaaatgg gcaaccatca
     5701 aaggaagtga cgaaacttgg caggccaaac gtgtcatgga acgcgtgcgt tatttggcta
     5761 gagagtttca tttagaaggg gctaaccgcc gttaagtttg agatagcaat caagaagaat
     5821 agctacacgt atgagtgagt gaaagaaaag gagaatcatg gttttaccta ccgttgccat
     5881 tgtgggccgt ccaaacgtgg gcaagtccac cttatttaat cgaatcgcag gagagcgcat
     5941 ttcaattgta gaagacgttg aaggggttac tcgtgaccgt atttatgcta ctggggagtg
     6001 gcttaaccgc caattttcat tgattgatac aggtggtatt gatgacgttg atgccccgtt
     6061 tatggagcaa atcaagcacc aggctcaaat tgccatggaa gaagctgatg ttatcgtttt
     6121 tgttgtttct ggcaaagaag gggttacgga tgcagatgaa tatgtgtcta aaatcctata
     6181 ccgaaccaat actccagtga ttttagctgt caacaaagtg gataatcctg aaatgcgtaa
     6241 tgacatttat gatttttatt cacttggttt aggggatccc tatccagttt cttctgttca
     6301 tggtatcgga acaggggatg tcttggatgc gatcgttgaa aacttgccag tagaagaggc
     6361 tgaagaaaat gatgatatta ttcgcttcag tttaatcggt cgtcctaatg ttgggaaatc
     6421 tagcttgatt aacgctattt taggtgagga ccgtgttatc gctagccctg tcgcagggac
     6481 aacacgtgac gctattgaca cgcattttac ggatgctgac ggtcaagaat ttaccatgat
     6541 tgacacggct ggaatgcgca aatctggcaa gatttacgaa aatacagaga aatattctgt
     6601 catgcgtgcc atgcgtgcca ttgaccgctc cgatgtggtc ttgatggtta tcaatgctga
     6661 agaaggtatc cgtgaatacg acaagcgtat cgctggcttt gcacacgaag ctggtaaagg
     6721 aatgattatc gtggttaaca aatgggatac cattgacaag gataatcata ccgttgctaa
     6781 gtgggaagcc gatattcgtg accaattcca atttttgaca tacgctccga ttatttttgt
     6841 gtcagctttg acgaagcagc gcctcaacaa attgcctgac ttgattaaac gtatcagtga
     6901 gagtcagaat aaacgtatcc catctgctgt cctcaatgat gttatcatgg atgctattgc
     6961 cattaatcca acgccaacgg ataagggtaa acgtttgaaa attttctatg ccactcaagt
     7021 gtcagtaaag ccaccaacat ttgtcgtctt tgttaacgaa gaagaattga tgcacttctc
     7081 ttatctacgc ttcttggaaa accagattcg cgcagccttt acctttgaag ggacaccgat
     7141 tcatctgatt gctcgcaaac ggaaataaga aatgaacatc aactaaaatg ctgacctaag
     7201 aaataatctc atcattgata aggactgtgt tgtgtatgtt acaggagaca gtccttttta
     7261 taataccttt aagggtgtag acggaagatt agtagcaaac aaagaaaaag gattttgaca
     7321 agaaatatca gtcggttttt gctataatga aaggataagc aaaaaggtgg aaaaatgatg
     7381 gctagattaa ttccaggacg agtaaggaat gaaggtatta agctttatga gcaaggttta
     7441 gtctcattcc aagatgataa taaaggcatt cttcaaatag aagtggagac ttatcaggtt
     7501 caatatggag cagatgatga ggatatcact tgtcaatgcg acacatttca tatgaaacat
     7561 tattgcaagc atattgcagc agtcgagtat tttttaaaaa atgatcaaaa aggtaagctt
     7621 tttttaaaac agttaacaaa tcaaacaaaa atcaaagaaa caacaaagaa aatgacatct
     7681 tttggtagtc tctttttaga tagcttagcc atgaatgaag atgattctgt taaatacagg
     7741 ctgtctgctc ttggtagtcg aagccctttt tcgtcagatt actggtggag tctgaaaatc
     7801 aatcgtttac cagatgatcg ttcttatgtg attcgagata ttaaaggctt tttgcagctt
     7861 attaaaaaag aaggctttta tcagattgga aaaaattatt ttgaacaact ttcatggctg
     7921 cagtttgacc catccagtca agaccttatt gagtttttat ggcgcttagc atctaatact
     7981 gacaaagggg acaacgagaa catttttcca aatcatgcaa ggcatttgcg cttacctagt
     8041 ggtttttttg aagaaggtat tcactatttg acgtcacttt atgactttac ttttgagggg
     8101 ccttctcaaa cttatcatca tctttttgtc cgttctttgg aagcagaggc aggcctttat
     8161 gagtttaaag tggaagtgta tcgcaaatct attgaattgc aaattgctga aaaaaatgtg
     8221 caatacctct ttgataatga ctatcttctt tatcaagata ccttttatca cttgacgtta
     8281 aagcaacgaa aaatggttca ggctattcgt agtctgccca ttgaggccga cttggctaaa
     8341 catattcatt ttgatttgga tgatcatgcg aaattagctg caagcttatc agattttaaa
     8401 cagatcggtc tagtagaggc tcctaaaagc tttgctatta gagactttga ggtgactttt
     8461 caatttgatt tgttaaatcg agatgaaatc agttgtcagc ttatgtttga ttatggcaat
     8521 taccaagtta gtgacaaagc aagtttagaa gcacttcctt ttgctagtca tttaaaaaaa
     8581 gaagaaaaaa tcaatcgttc tttacttgct tttggcttct cgccacaatt ttactcaaaa
     8641 aagcgactaa caagtgcaaa agagctctat actttttttg aagaaacagt tccatgtttt
     8701 gagcgcttag gtaatgttgc gttgagtaca gctatccaag cattacaagt gaaagaaatg
     8761 cccaagatcg ctattagacg caatcaggga ttactagata tttcgtttga tttttcaacc
     8821 attattgaaa atgatattga ccaagctgtt acagctcttt ttcaaaataa tccttatttt
     8881 gtgagtcaaa caggtcaatt agtggttttt gatgatgaaa ctcaaaaagt tagcaaaagt
     8941 ttacaagaat taagagcaag acagctaaaa aatggtcact tgcaactgga tggcattaga
     9001 gctctacaag tatccaaact ttttgaaggc atgacatctg ttcatttttc aaaggaatta
     9061 gaagagttag cctatcattt gcagcatcct gaaacttttt caataaaacc gttaccagtt
     9121 aaggctcaaa tgcgagatta tcaacgcaac ggtgttcagt ggttatcaat gctcaatcat
     9181 tatggctttg gtggcatttt agcagatgac atgggacttg gaaaaactct ccagacactt
     9241 gcttttttag ctagtcacct gaaatcagat agtaaagtgc tcattttatc tccatctagt
     9301 cttatctata attggtttga tgaatgtcaa aaattcacac ctcagctaga tgttgtagtt
     9361 tcttacggac ttaaacaaat cagagatcaa atcatcgaag aaggtcatca aataaccata
     9421 accagctatt cctcctttag acaggatttt gagacctatc aagcgtttca ctacgattat
     9481 cttatcttag atgaagctca agtgataaaa aatgcgcaaa ctaagatttc acactgttta
     9541 cgtgcgttta atacagccaa ttgttttgcg ctttcgggca ctcctattga aaataaaatg
     9601 cttgaaattt ggtccatttt ccaaattgtt ttgccagggt tgctaccgac taaaaaagag
     9661 tttttaaaat taacagctga gcaagtatcg cgctatatca aaccctttgt gatgcgtcgt
     9721 aaaaaagaag atgtcctacc agaattacct gatttaattg aaactaatta ttcaaacgag
     9781 atgacagatg aacaaaaagc tatttactta gcgcagttaa ggcagatgaa agatcaaatt
     9841 cgaaattcta gtgatgtgga tattagccgt caaaaaattg agattttatc tggtatcact
     9901 cgattgaggc aaatctgtga cactccaagt ctctttatgg attaccaagg caaaagtggt
     9961 aagctagata gtttgcgaat cttattgaca caaattaaag aaaatggtca tcgggctttg
    10021 attttttctc aatttagagg gatgctggac ttggccaagc aagagatgac tgctctagga
    10081 ttgacctctt atcaaatgac gggatctacc cctgccaatg aacgtcaaga aatgactaga
    10141 gcctttaata acggttccaa ggatgctttt ttgatttccc tcaaggcagg tggagttgga
    10201 atcaaccttc cgggagcaga cacagtgatt ttaatcgatt tatggtggaa tcctgctgtt
    10261 gaaatgcagg caatcagtcg cgcctaccgt attggtcaaa aagaaaacgt tgaagtatat
    10321 cgcttaatca ctcgagggac gattgaagaa aagattttag agcttcaaga aagtaagcgc
    10381 aatttggtga ctacagtttt ggatggcaat gaaagccgag caagtatgtc cattgaagaa
    10441 ataaaagaaa ttttggggct aaacactcat taatcttcct tgattttagt gacattaaaa
    10501 accctttaaa gaagatgaca ccaaaagcac cttatataac ctcgttaaaa cgcttctttt
    10561 tgaaatatct ttattgatat agcgattgtc atttgctagt aatgattaaa acatttagaa
    10621 aaagacaagt catagaatca tcaaggaatg ctttgaaaaa taacagtatt taagttataa
    10681 tagaggatgc tacaagaagg gagaatcaaa aaaggatggt aaaaaatcag tttcctttag
    10741 ttgcagatgg cattgctatt agtgatccag caaagcaaat ggctctgtat gaaaatgaag
    10801 atctcattac caacatccgt ggctattacc aggacaagga gtatgatgat attgctagaa
    10861 atgaggagtt tactgctaaa gcaacttctc ggcaggcccc ttctagtaag cgcttttgta
    10921 gcaatgatga aaaacaccat tatgtcaaag aggcgcgtca aaaagcaaag caagatctca
    10981 aggaaaaaag acaggcttat cttgccaaag aaatggccta tgttcctaaa caggtttcta
    11041 aaaagcaaca gcctgcggat tcttctcctt ctcaaaagca agcaacaaca gaaatgagtc
    11101 gttttacaaa aaagcttcat caagacaact atattttagc tgagttgcca aaagaatata
    11161 aagagccaaa aaatcttcct caacaaggaa ctacaaaaaa aaataattac gattttttaa
    11221 aatccagtca aatttacaat aataaagaaa tgagacaaca acgagaaaaa acaattgcgc
    11281 aagagcttaa tcttagtagg tttgaggatc ttccttaata aatctactca gcgtatgcag
    11341 atgacaaagg agttaagatg tcaaaaacgt atcattttat cggaattaaa gggtcaggaa
    11401 tgagtgcctt ggcccttatg ttgcatcaaa tggggcataa ggtgcaaggt agtgatgttg
    11461 aaaaatacta tttcactcaa agaggattgg aacaagcggg catcactatc ttacctttta
    11521 gcgaggacaa cattacgcca gatatggaac ttatcgttgg gaatgccttt cgtgaaaaca
    11581 acaaagaagt ggcctatgct ttgagacacc agattccttt caaacgttat catgactttt
    11641 taggtgattt tatgaaatca tttatcagtt ttgcagtagc aggtgctcat ggtaaaactt
    11701 ctacaacggg tttattgtca catgtcttga aaaatatcac agacacatct tatttaattg
    11761 gtgatggtac tgggcgtgga tctgctaatg cacagtattt tgtttttgaa tcagacgaat
    11821 acgaacgcca ttttatgcct tatcacccag aatactctat cattactaat attgattttg
    11881 accatcctga ttatttcaca ggaattgctg atgtgcgaaa cgctttcaac gattatgcta
    11941 agcaggtcaa gaaagctttg tttgtttatg gtgaagatga cgaactaaaa aagatcgaag
    12001 cacctgcacc tatttactat tatggttttg aagagggcaa tgattttatc gcttacgaca
    12061 ttacgcgtac tacgaacgga tcggacttta aggtaaaaca ccaaggagag gtcattggtc
    12121 agtttcacgt acctgcttat ggaaaacata acattttaaa cgctacagca gtaattgcaa
    12181 acttgtttgt agccggcatt gatatggctc tagtggcaga ccatcttaaa actttttcag
    12241 gtgtgaaacg tcgttttaca gagaaaatta tcaatgacac tattattatt gatgactttg
    12301 ctcatcatcc aaccgaaatt gtggcaacaa ttgatgcggc tagacaaaaa tacccaagta
    12361 aagaaattgt agcaatattt cagccacata cttttactcg cactattgcc ttattagagg
    12421 attttgcttg tgctttaaat gaagctgaca gtgtttattt agcccagatt tatggttctg
    12481 cacgtgaagt agataaaggt gaagttaagg ttgaagattt ggctgctaag attatcaaac
    12541 catcacaagt agtaacggtg gaaaatgtct ctcctcttct tgatcacgat aatgctgttt
    12601 atgtttttat gggagctgga gatattcagc tatatgaaca ttcttttgag gaattacttg
    12661 caaatttaac taaaaataac caataacatg tcctgcctta ggcaggtttt ctcattctaa
    12721 aggagtttga tatgttgatt agacaggttc aaggatccga tttggaagtt attgctacca
    12781 ttgaaagtga caatttttca ccacaagaag caacgacaag agcagttctt gaagagcgca
    12841 tacggctgat accagatact tttttagtgg cacttatcga tcaggaaatt gttggctata
    12901 tcgaaggtcc agttgtcact actcctattt tggaggattc tttgttccac ggggtgacta
    12961 aaaatcctaa aactggaggc tatattgcta ttactagttt gtccatagct aaacattttc
    13021 agcaacaagg ggttgggaca gctttgttgg cggcactaaa agatttagta gtagcgcagc
    13081 aaagaacagg tcttattttg acttgtcatg attacttgat ttcctattat gaaatgaatg
    13141 ggtttattaa ccaaggaatt tcagagtctc aacatggtgg aaccctttgg taccaaatga
    13201 tttggaaact ttagaatcac agctgattca actgttggtt tgttaaaaat aagtaacaaa
    13261 tgcatcattt aattgcaatt aggatacttt taagttataa tggcggttga ttatatttag
    13321 gaggattggc tttgaccgat tttaaggata aagaccaaca agatcagcag cgaagcttta
    13381 aagagcagat tcttgctgag ttggaaaaag caaatcaaat cagaaaagaa aaagaggaag
    13441 aactttttca aaaagagttg gaagctaaag aagcagctaa gagaacggcc cagctatatg
    13501 ctgaatataa aaggcaagat gcttttcaaa aagagtctat agcacataac aataagacag
    13561 ctaaacactt tcaagctata aaaggtgcgg taatgacttc agaagcgctt aaaccgactt
    13621 tactttctga aaaagaaaac tcatctttaa aaacgacaaa taagagagtc gtgcaggcaa
    13681 atgagcttca agagactgcc tctaaagaat ctcaagtacc gttaactatt gagaaaggtc
    13741 attcagtgag acgaaaatta agcaaacgcc aacagactga gcgagctgct aaaaagattt
    13801 caaccgtttt gattagttct attattataa cccttttggc tgttactcta gcaggagcag
    13861 gctatgttta tagtgcttta aatcctgttg ataaaaatag tgatgccttt gttcaagttg
    13921 agataccatc tgggtcaggc aataaattga ttggtcaaat tcttcaaaaa aaaggtttaa
    13981 tcaagaatag cactgttttt agtttttata caaaatttaa aaactttaca aattttcaga
    14041 gcgggtatta caatctgcaa aaaagtatga gtctagaaga aattgctagt gctttacaag
    14101 aaggtggtac agcagaacct accaagccat ctcttgggaa gatcttgatt ccagaaggat
    14161 acacgattaa acaaatagct aaagctgttg agcataatag caagggaaag accaaaaaag
    14221 ctaaaacacc ttttaacgag aaggattttt tggatttagt cacagatgag gcttttattc
    14281 aagatatggt aaaaagatat ccaaaattat tagcaactat cccaactaaa gaaaaagcta
    14341 tttaccgttt ggaaggttac cttttcccag caacctataa ctattacaaa gaaactacca
    14401 tgagagaact tgtagaggac atgctggcag ctatggatgc tactttggta ccctattatg
    14461 ataaaattgc tgctagtggt aagacagtca acgaggtatt gaccttggcc tctttggttg
    14521 aaaaagaagg ttcaacagac gatgacagac gtcaaattgc aagtgtcttt tataaccgcc
    14581 ttaatagcgg aatggcacta caatctaata tagctatttt gtatgcgatg gggaaacttg
    14641 gtgagaaaac aaccttggct gaggatgcta ctattgacac caccattaat tctccttata
    14701 atatttatac caatacaggt ctgatgcctg gtccagttgc tagctcgggg gtttctgcaa
    14761 ttgaagcaac cctaaatcca gcctcaacgg attatttata ctttgtggcc aatgtccata
    14821 ctggtgaagt ttactatgca aaaacatttg aagaacactc tgcaaatgtt gaaaaatatg
    14881 tgaatagtca aattcagtaa acacaaacat ggtgcttaag caccatgttt gtgtttctga
    14941 ttcgttcaaa aaagaaaaga gagaaaacaa ttatggcaga aaaaacgtat ccgatgaccc
    15001 taacggaaaa agaacaactt gaaaaagaac ttgaagaatt aaaacttgtg cgtcgtccag
    15061 aaatagtgga gcgtattaaa attgcacgtt cgtatggaga tctttcagag aactctgaat
    15121 acgatgccgc aaaagatgag caagcttttg ttgaaggtca aatttcaacg cttgaaacta
    15181 agattcgcta tgctgaaatc attgacagtg atgctgttgc aaaagatgaa gtcgctatcg
    15241 gaaaaacagt tatcgttcaa gaagttggaa caacagataa agatacctat catattgtag
    15301 gtgctgctgg agcagatatc ttctcaggaa aaatttcaaa tgaaagcccg attgctcaag
    15361 ctttgattgg taaaaaaaca ggtgacaagg ttagaattga atcaccagca gctacttatg
    15421 atgttgaaat catcagcgtt gaaaagacaa attaatcaga tgaagccatt atttgcctaa
    15481 cccctaagtg ttttggttgc taatggcttt tctagtatct tcaaatgagg ttgactgtta
    15541 ggaaggtcaa gtattaatcg cattgattat gagccaagag gactatagat caaagatcgt
    15601 tttcgatggt tatttaatac caaaagaaaa gaccagcacc ttaatgtact gaccccaaaa
    15661 agttggacac tatattttaa gtataggaag tgtataatct aaggaaactg acactaatga
    15721 ttgaccgttt tgcaagactt tattaatgat ttcttgcttt attttaaatg aataagtctg
    15781 atacgtttcc ttccttaaca ctatcgactc cataatgacc catcagtcaa acggtatatc
    15841 tcaaacgact acggtttact ccaaattgat tggctagttg aggccaagaa acacctgatt
    15901 tttttaatgc taaatttcta gcttatcttc atagcttaat ttcatacaaa aacaccccaa
    15961 tcgttagatt tttttgatat gtcccctttc actagatgag tccagtaaaa gggggcatat
    16021 cgttttttgt ctaacttttg gagtgcagtt cataattgtg ctaggttttt tgtctttctt
    16081 aacgctttcg ctgttttcca gcattacgat tggattttat ttttttaggc aagttagcct
    16141 gttccatgtt ttgtgaaggc gtaacatcct tacgagaaga agttgattgg tatgctttag
    16201 gcggggtttt agcgtattct tctttaattt gcttatgcaa tcgaggtttt aagaggtagg
    16261 ttgtgattaa ctgttgaata atactaaaga aaccaccaac tagccagtaa agaccaacac
    16321 cagcagggag tgagaaggac atgaagatca tcatgatagg catggtgtac atcatcgtct
    16381 tcatttgctc acgttgttct tctgaaacgg ccatcattga taaccatgat tggaagaagt
    16441 aaagagcagc gataattgct gttaacacta aactacggct accaagatca atgcccataa
    16501 aggtacttgt tgatactcct ttagtgtatt gagcggcaaa atacatggca gaaaagaatg
    16561 gcatctggat gagaagaggt agacatccaa tacctccaag agggttaatc ccatgagcac
    16621 gctgagcagc catcaattct gtttgggcag ccattttttc ttcctgacta cttgcctgtt
    16681 tgatacgttt gttgattggt tcaaaaacag gtttcaagaa ggtcattttc tcagattgat
    16741 aacttgcttt ccaagattga tatagaccta gtggtaagat aagtgttcta acaataatgg
    16801 ttacgatgat gatagccagt ccataaccta gtccagcatt gtttgcaaag taatcgataa
    16861 agtatgacat gggttttcca agaaattccc aaatcatacc tttgggattt ccgtgagcgt
    16921 ctcttcccac acagcctgtc aaggtgaata gaattgacag tgctagtcct gaaaagagga
    16981 tgcgatttaa tgttagtttc aatgtgttta tcctttaatt ttaatatact tatctatttt
    17041 acagtttttt tcagaaattc acaatagttc tgaaggccga tttttaattg cctatttgaa
    17101 agtcctgaaa gtcttcaaaa ttagccatgg tgacatccac ataagtgact tttgcccatt
    17161 tggatggtcc tttacgaact tcttgaataa atgtggctat tttatctgaa tcttttgatt
    17221 gggccagaat ttcaacggtc ccatcgttgt tattccagac acgaccataa atatctccta
    17281 tatctagagc taaagtatgg gtagcgtatc gaaaaccaac accttgtaca cgtccagaaa
    17341 caattaaacg tactttttgc ataagaaggg cctccttgct tttattatag cacatactta
    17401 gcaggaaatc tctttagcta attgtctact tcctgtcact tttactaaag acaagaatac
    17461 aaaaatgaga tagttagtag tactaacagt aaaagagatt tgtctatttt aaaaatagta
    17521 tcaaaaaaag gtcttttagg tctaaaacat tgaactgacc ccaaattgac catctaagaa
    17581 gttgatgctt tcaaatgctg tcgcgtatgg tataataggc gcatggttat tacatcaaaa
    17641 gcaaatcaac taattaagca aacaaaaaaa ttattgcaaa aaaagcatcg aaaacagtcc
    17701 tatttgattg agggctggca cctttttgaa gaagcccaaa aatcaggaca agtttttcgc
    17761 cacatctttg ttttagagga aatggtggag cgtctagcag gtgagcaaga actcgttatt
    17821 gttagcccac aagtgctcaa ggagttgact gactctccta gtccacaagg cattgttgct
    17881 gaggtggaga tacctaagtt agcctttcca tcagattaca aagggaaata tttggtttta
    17941 gaggacgtcc aagacccagg aaatctgggg accatcattc gtacagcaga cgctgcacgg
    18001 tttgatgggg tctttctttc tgaaaaatca gcagatattt acaaccaaaa aaccctacgt
    18061 tccatgcaag gtagccattt tcatttacca atttggcgaa cagatgtcta tcagctttgt
    18121 cgagagctcc aagagtatga aactcccatc ctagcaacaa ctctgtcaaa gaaatctgtt
    18181 gactataaaa gtctcacaca tcatgaaagg ttggctttgg tgttgggaaa tgaaggccag
    18241 ggcattagtg ctgaaatggc agccctagca gaccaactgg ttcatattac aatgcctggt
    18301 caggcggaaa gtttgaatgt tgccgtagca gcaggaatcc tcattttcag cttgatttaa
    18361 gctaatatga tataataacc aagtgaggta attatgttat gcattataca gaagataaag
    18421 aatatatgga acacgtcggt catttgatcg ctcatcctcg ctttcaaaaa ttgagtcata
    18481 ttgttcagca ccaacattca acgcgtcttg aacattctat caatgtttct tattcaagtt
    18541 ataaattagc caagcgattt ggttgggatg ctaaaagtac agccagaggg ggcttacttc
    18601 atgatttctt ttattatgat tggcgcgtga ccaagtttaa taagggtcat gcctgggttc
    18661 atcctcgtat tgctgtacga aatgcgaaaa agttaacaga actcaataaa aaagaagagg
    18721 atatcattct aaaacacatg tggggagcga ccattgcttt tccgcgttac aaggaaagtt
    18781 atattgtgac aatggtagat aaatactggg ctgtcaagga agcagtgacg cctttgagac
    18841 aaaaatggtc taatcgacgg tttcttcacc gcaagaccct ccaaagtcat aatcgttaag
    18901 taaaggagtt attatgaacg atcacgttat ttatacacaa tcagatgtgg gattaaatca
    18961 gttttttgct aaaatttata gtctagtggg aatgggagtg ggtttgtctg cttttgtttc
    19021 ttatttgatg ctttatccat ttagagaaaa tttaatctct attcttgtta atcagccgat
    19081 gatttactat ggtgctgcga ttattgaact tattttagtt tttgtagcca gtggtgcagc
    19141 acgaaaaaac acaccagctg cattgcccat ttttcttatc tattctgcgc taaatggctt
    19201 taccctaagc tttattattg tggcatacgc tcaaacgaca gtttttcaag catttctttc
    19261 ttcagcagct gtgttttttg ccatgtcaat cattggtgtc aagactaagc gggatatgtc
    19321 aggtcttaga aaggctatgt ttgctgcttt gattggtgtt gttgtggcta gtctgattaa
    19381 tctttttatt ggtagtggga tgatgagtta tgtcattagt gtgatttccg tgctaatttt
    19441 ctcaggtctc atcgcatcag ataatcaaat gattaagcgc gtttatcaag caacaaatgg
    19501 ccaggttggt gatggctggg ctgttgctat ggccttaagc ttgtatttag attttattaa
    19561 cttatttatc agccttcttc gtatttttgg aagaaatgat taacattaaa aagtgagtat
    19621 aactactcac tttttaatgt taacaggata agtaaactgt aaggaagaaa ttcgagatag
    19681 atataagatg aggctgctaa aagacctagt gttaatttgt aaactctttc tttttttggt
    19741 atactattaa ggattaaata cgaggagaat atagatagat atgtcaacag ctatttggat
    19801 tttattactt attgttgccc ttggggtagg tgtttttggt gggattttta ttgcccgtaa
    19861 acaaattgaa aaagaaattg gtgagcaccc acgtttaaca ccagaagcta tccgtgaaat
    19921 gatgagccag atggggcaaa aaccaagtga agcaaaaatc caacaaactt atcgtaatat
    19981 catcaaacaa tcaaaagcag ccgtatccaa aggtaaaaaa taaaagctaa ttgttatagg
    20041 ggaaaccttt gtgatagtta gaagtgaaca gtctttgatc tcaatgttca gtagcacttg
    20101 tcttatcagt tagatttgtc aactgttcag ggtttaaaaa gaaactcagt tggctatttc
    20161 caactgagtt tttcatatcg tcagtaagga agtaaaggta atggatacaa gaccaattgg
    20221 gtttttagat tctggtgtgg gaggtctgac cgtcgtttgt gaattgattc gtcagctccc
    20281 tcatgagaag attgtatata ttggtgattc agcaagagcc ccctatggtc ctagacccaa
    20341 aaaacaaatt aaggagtata cttgggagct agtcaatttt ttgctgactc aaaatgttaa
    20401 aatgattgtt tttgcatgca ataccgcaac agcggtggct tgggaagaag taaaagcagc
    20461 tttagatatt cctgttttag gggttgtctt accgggggca agcgcagcta ttaaatcaac
    20521 gacaaaaggc caggttgggg tcatcggaac cccaatgaca gtggcttcag acatttatcg
    20581 caaaaaaatc cagctattag caccatctat tcaagtaagg agtcttgctt gcccgaagtt
    20641 tgtaccgatt gtggaatcaa atgagatgtg ttcgagtata gctaaaaaaa tagtttatga
    20701 cagtctagca ccattagtcg gtaaaataga tacccttgta ctaggatgta ctcactatcc
    20761 cttgttacga ccaattatcc aaaatgttat ggggccatct gttaagctga ttgacagtgg
    20821 agcagaatgc gtccgagata tctctgtctt attaaactat tttgacatta acggtaatta
    20881 tcatcaaaaa gctgtcaaac accgcttttt tacaacagca aatcctgaga tttttcaaga
    20941 aattgcctcg atttggctga aacaaaaaat aaatgtggag catgtcacct tatgagtgaa
    21001 aaaatttacg aatacaaaga tgaaaataac tggtttatcg gaaagatgac tggtcataat
    21061 ttaatatcgg gctggggagt caaacacacg acaatcaaaa aaatagatga ccttttagac
    21121 ggtattgccg caacgttaga ttgggaaaat cctaaaggtt acgatgtttc ggtggtgcgt
    21181 tatcagtctc ctttatctct catcaccttt attatcgata tgattaacca agaaacccag
    21241 cgtgagatta aggtgattcc ccatgcaggc acgattttat taatggaaaa tgcgaaactg
    21301 cttgcagttt atctcccaga aggtggagtt tcaacggcta cattttttgc gactagtgaa
    21361 cagggatttg gggatactat tttaatcgct acacgaaacg agggaaaaac caaagagttc
    21421 cgcaatctgt ttggccagct aggttatcgt gtggaaaatc tcaatgatta tccagaattg
    21481 ccagaagtgg cagaaactgg tactactttt gaagaaaatg ctcgcttgaa agcagaaacg
    21541 atttcacgcc tcacaggaaa aatggtatta gcagacgatt caggactaaa agttgatgcc
    21601 ttgggtggtt tacctggggt ttggtcagct cgtttctcag gaccagatgc cacagatgcc
    21661 aagaataatg ccaaactctt acacgaatta gctatggtgt ttgaccaaaa gaagcgctca
    21721 gctcaatttc atacgacttt ggtagtggct gctcccaata aagatagttt ggttgtggaa
    21781 gctgagtggc caggctatat tgctacgcag cctaagggag aaaatggttt tgggtatgac
    21841 cctgtcttta tcgtcggaga gactgggcgt catgctgcag aattagaggc tgaccaaaag
    21901 aatcagttgt cgcatagagg tcaagcagtt cgaaaattaa tggaggtctt tccagcatgg
    21961 caagcaaaac aatcatagta atgagtgact ctcatggtga ccgagatatc gttcaagcca
    22021 ttaaagacaa gtatcttggt caagttgatg ctattttcca taatggggat tctgaattaa
    22081 acagctcaga tcctatttgg gctggtattt atgtcgttgg cggtaattgt gactatgata
    22141 caggttaccc tgatcgttta gtaactcaat taggcacagt tacgattgcc cagacacatg
    22201 gtcacttgta ccatattaac ttcacttggg ataaactgga ctactttgct caagaagtag
    22261 cagcagatat ttgtctatat ggacacctac accgtccagc tgcttggcaa gttgggcaaa
    22321 ccctctttat gaatcccgga tcagtaacac aacctcgcgg agaaattaac gagaaattat
    22381 atgctcgtgt tgaactaact gatactcaaa ttaaagttga ttactttaca agagatcata
    22441 aactttaccc atcactgtct aaggaattta aacgatgatc gcaaaagaat ttgaaacatt
    22501 tttaatgagt catttagata attatcttat cccggaacaa gacttggcta tttttatcga
    22561 cacacataat gcagaccatg tgatgttact attggtgagc aatggttttt ctagagttcc
    22621 tgttatcaca cgggagaaaa aatatgtggg aaccattagc atttcagata ttatgatgta
    22681 ccaatctaaa aggcagctga cagattggga aatgagtcaa acagatattg gcgagatggt
    22741 taataccaaa attgaaacca ttagtattac ttctagccta acagaaatta tgcacaagtt
    22801 aattgatttc ccattcttac cggttgtaga tagagcgaat cgctttgttg ggattattac
    22861 gcgaaaatct atattaaagg ctgtcaacag tcttttacat gattttacag atgattacac
    22921 gattataaaa aaatgaagag ttatattgaa ccttttattg ctagcaaggc tttatctcaa
    22981 aatagtcaaa aagcttatcg atacgatttg caacagtttt gtcaactggt tggtgaacgt
    23041 gtcaatcaag acaaactatt actttatcaa aatagcatag ctaacctaag tttatctgca
    23101 aaaaaacgca aattatcaac agctaatcaa ttcttatatt acttgtatca aataaagtat
    23161 ttaaatagct attttcgatt aacagatacc atgaaagtga tgcggactga gaagcagcaa
    23221 gctgctatta ttaatactga tattttctat caaaaaaccc cttttgtgtg ggggcaatta
    23281 atcagcttat taatattgga gctgggttta actccgagtg aagtagctgg tattgaagta
    23341 gctaacttgg atttaaattt tcaaatgttg acgctaaaga ctaaaaaagg tgtgcgtgta
    23401 ttacctttgt cgcaaatact aatccctttt ttagaacaac aactcgttgg caaagaagtc
    23461 tatctttttg aacatcgagg catccctttt tcaagacaat ggttttttaa tcacctaaaa
    23521 acctttgttc gaagtattgg ttatgaagga ttaactgctc aaaaattacg agagcaattt
    23581 attttgaaag aaaaactagc tggtaaatcg ataatagaac ttagcgatat tcttgggctg
    23641 aaaagccctg tgaccttaga aaaatactac aaatcataat ggatataaaa cttaaagatt
    23701 ttgaaggtcc tcttgattta ctcttgcact tggtatcaca gtacaaagtt gacatctacg
    23761 aggttcctat tgttgaggtt attgagcagt accttaatta tattgaaacc ttacaggtaa
    23821 tgaaacttga agtggctggt gactacatgc tgatggcaag tcagctcatg ttaatcaaaa
    23881 gtagaaggct attaccaaag gtggtagaac acatcgaaga agaagattta gaacaagatc
    23941 tgttagaaaa gatcgaagaa tatagtcgct ttaaagcagt tagtcaagcg ttagcgaagc
    24001 aacatgacca aagggcaaaa tggtattcaa aacctaaaca agaattaatt tttgaagatg
    24061 ctatcttaca agaagataaa acggtgatgg atctatttct agctttttca aacataatgg
    24121 cagctaaaag agcagtttta aaaaccaacc atacagtcat cgagcgtgat gattataaga
    24181 ttgaagacat gatggcttct attaaacagc gactggaaaa agaaaacgtt attagcttat
    24241 cagcgatttt tgaggaatgc caaacattaa atgaagttat cagtatattt ttagctagtc
    24301 tagaattgat taagcttcac gtggtatttg tagagcagct ctctaatttt ggggctatca
    24361 tactacgaaa ggagaagaaa tgacgtattt gtcacagata gaagcgctgc tatttgttgc
    24421 aggtgaagaa gggttaagtt tgcgacatct agcgtctatg ttatcattaa cgccaacagc
    24481 tcttcaacag caattagaaa aactctctca aaagtatgaa aaagatcaac attctagtct
    24541 ttgcttgata gaaacagcta atacctacag gctagtaact aaagaaggtt ttgcaggact
    24601 cttgcgtgct tatgccaaaa caccaatgaa ccaaagttta tctcgtgcga gtttagaggt
    24661 cttgtctatt gttgcataca aacaacctat tacacgaata gaaattgatg acattcgagg
    24721 ggtaaactcc agtggagctt tgagtaaatt attggcattt gatctcatta gagaagctgg
    24781 caaaaaagat gtggttgggc gtcctcattt gtatgcgaca acagattatt ttctggacta
    24841 tatgggaatt aatcatttag acgaattgat tgaagtatct gccgttgaac cagcagatga
    24901 agagattgct ctttttagga cacaagactg agttttgctt gtcatcaaat agcaaatggc
    24961 gtttacaaaa ggatttgttt taaggcgttt tagttgaaaa aaagccttaa actagggaca
    25021 acaaaaagtt gctcaaaaga aagaagaata taatgcgaat taacaaatac attgcccatg
    25081 cgggtattgc cagtcgtcgt aaggctgaag aattgattaa gcaaggctta gtaaccttaa
    25141 atggtcaggt tattacggat ttagcaacca ctgtaaaatc gggagacgtg gttgaaatag
    25201 aaggaagtcc tatttacaac gaggaaaagg tttactattt gcttaataaa cctcgtggcg
    25261 ctatctcaag tgtgtcagat gacaaaggcc gtaaaacggt actggattta ctacctcagg
    25321 ttaaagaacg catataccct gttggacgat tggattggga cacatcaggg gtccttattt
    25381 taacgaatga tggtgatttt acagatacta tgattcatcc tagaaatgaa atagacaagg
    25441 tttatctggc gcgtgtcaaa ggtattgcaa cgaaagaaaa tcttcgtccg ctaactcgag
    25501 gaattgttat tgatggtaaa aagaccaaac cagctcgcta caatattgtt cgtgtagaag
    25561 ctgataagaa tcgatcgatt gtagaattga ctatccatga agggcgaaac catcaggtta
    25621 aaaaaatgtt tgagtctgtg gggcttttgg ttgataaatt atctcgtaca cgttttggga
    25681 ccgtcgactt aaagggacta agaccaggag aagcaagacg cttaaataaa aaagaaataa
    25741 gtcaactgca taacttagct aatacgaaaa aatgatgaaa aaactattaa tagtatctgt
    25801 taaagcttat caaaaataca tttctccttt atctcctcct agttgtcgct acaagccaac
    25861 ctgttcagct tatatgttaa ctgctattga aaaacatggt actaagggaa tactaatggg
    25921 gatagcaaga atattaagat gtcacccttt tgttgctggt ggtgtcgatc ctgttccaga
    25981 agattttagc ctaatgagaa ataaaaacac gtctaaaaac gctgaaaagg catgacttta
    26041 tcaaaaaatg gcttgacatt gatagctgaa gtgttataat ggaaaacaag cattcgttgg
    26101 tttaaatcaa acctgttatg atttaagtta gcgagtcacc atcaaccaca ttgtttgctg
    26161 agcatgactc cgagcagtgt ggttttttaa attaaaaaga gtaaatgaga ttatgacgac
    26221 aaaagaactt atcaacaaaa acgacaaagt aaaaaaggct cgcaatcata ttgtcctgtt
    26281 tcaaccccaa attccacaaa atactggtaa tattgctaga acatgtgctg caactaatgc
    26341 acctttacat attattaaac ccatgggatt tcctattgat gaccgcaaaa tgaagcgtgc
    26401 cggcttagat tactgggaca agttagaact gcatttttat gatcatttag aacagtttat
    26461 caatcaatgt cacggacagc ttcacctgat tagcaagttt gcagttaata attattctca
    26521 agctacttat gctgatggcg attcccatta tttcttgttt ggacgagaag atacaggcct
    26581 gccagaagat tttatgcgag aacatgctga aaaagcactt cgtattccga tgaatgatga
    26641 gcatgtacgg agtttaaatg tctcaaatac ggtttgtatg gtgatttatg aagcccttag
    26701 acaacagggc tttcaagggt tagagttaaa gcatacttac gaacacgata aactaaaata
    26761 atggactttt taaaagcgtc agtttttggc aacatgcgtt gtcagtagct gacgctttta
    26821 aaaatttagt caaagctatt gctatctagg tttaaatctg ctatgctaga agtgtcttca
    26881 gggcagggtg tgattcccga ccggcggtga ctaagtaact agaagtccgc gagcgcaagc
    26941 tgatgtggtg taactccaca accgacagta tagtctggat gagagaagac cgggcttttt
    27001 gtgtaggctt attttagcta cacaataacg acggaacttc tttcaatttg aaaaaattgg
    27061 aggaattttt taatgtcaaa aacacataaa atgattatga ttggtatact ttctgcgata
    27121 tcatttctat tgatgctagt tagctttgcc attattccag gcgcagcttt tttaaaaatt
    27181 gaatttagta ttattcctgt tttatttggt ttaatgatta tggacttaaa gagtgcttac
    27241 ttgattttgt tattgaggtc cttattgaaa ctctttttga acaatcgtgg agttaatgat
    27301 tttattggtc tcccaatgaa tattatcgct atcgctttat ttgtgacggc ttttgcctta
    27361 gtttggaacc gtcaaaaaac gcttagtcaa tatgtatttg ctagcttact aggcacaggg
    27421 ttattaacgt ttggcatggt tgttctcaat tatacttttg ccattccttt atatgctata
    27481 tttgcaaata ttgatatcag agcttatatt ggtgttacta agtatatgat gactatggtt
    27541 attccgttta atcttgttga agggttgata tttgcaatta ccttttattt tgtgtatatt
    27601 gcaagtaaac caattttaga aagatactta cactaatatg atgacaaaca aacaaactca
    27661 ctttttaata gcttcttttg cattgctaat ctttgttata ataggctata cggtcaaatt
    27721 ttttcctgaa cgtttggcac ttctagataa tactattcaa gcagagatta gaggcaacct
    27781 tcctatagtg ctgacgcaat tctttagggg tgttactgta tttggaaatg tcatgacaca
    27841 agttttgtta gttattgtgt cggtcctagt tttattcttt atgaagtgga aaattgaagc
    27901 cttgtttatc ttatcaaatg gtgccattgc agcatttttg attacgactc tcaagctctt
    27961 ttaccaaaga ccaagacctg ctattgagca tttggtttat gctggtggct attcgtttcc
    28021 cagtggccat gcgatgggta gcatgctgat ttttggtagc ctattgatta tttgttatca
    28081 aaggctacat tcaaaactat tacaatttgt gactagcatg atctttataa tactaatttt
    28141 agtaattggg ttatctcgta tttatctagg ggtacactac ccaagtgata ttttagcagg
    28201 ctttgtatta ggatttggga ttttgcagtt tatttatcct ttttataagc aaaaacgatt
    28261 tgaatggcga tttcttttga aacaagacta atttttcttg agaagacata gggcttattt
    28321 aaatcattta gaataacatt ggcaagcatc accccttgat tgccagtgtt attttagttt
    28381 tagttagatg atgtgagctg atgattagta aatacgttta gataaaaaac gattaaagtg
    28441 ctatgtaacg tcagaaaaga cgtgattttt gttgatctgt aatcattaag taagctgatg
    28501 tagaagtagt gaattctaat gactgagcta tcttttgcat agatgactat cttgctagta
    28561 atgatttgtg ctacaataga aacatgaaaa aaagatatca gacattaaac gagcattacc
    28621 gtcaactatt tggcgcaaaa atgtttaaag ttccgattga tgccggcttt gattgtccca
    28681 atcgtgatgg aacggtagct catggaggat gtactttttg cacagtatca ggttctggag
    28741 atgctattgt agcaccagat gctccaatta aagagcaatt ttataaagaa attgatttta
    28801 tgcaccgtaa atggcctgat gtcaatcgct atttggttta ttttcaaaac tttaccaata
    28861 ctcatgatac agttgatgtg attcgcgatc gttacgaaca agctattaac gaacctggag
    28921 ttgttggcat taatattggc acacggcctg actgtctacc agatgatacc attgcttact
    28981 tagcagaatt atcagaacgc atgcatgtga cagtggagct aggcttacag acaacttatg
    29041 aagaaacttc taggctaatt aacagagcac attcttatga tttgtataaa gaaacggtga
    29101 ggcgactaag gcattacccc aacattaata ttgtctcgca cttgattaat ggattgccaa
    29161 aagaaactca tgacatgatg cttgaaaatg tgagacgctg tgtaacagac aatgacattc
    29221 aaggaattaa attgcatttg ctgcatttga tgaccaatac tagaatgcaa agagattatc
    29281 atgaaggacg cttaaagttg ttgagtcaaa aggactatgt ttcgattatt tgtgaccagc
    29341 tagagattat ccctaagcat attgtgattc accgtattac aggagatgcc ccacgcgata
    29401 tgttgattgg gcccatgtgg agtctaaata agtgggaagt attgaatgcc attgataaag
    29461 aaatggaaag acgaggaagc tttcagggct gtaaagttga ctaatttgtc tcattttgac
    29521 ggatttggat aggagaaaag tgatgctaaa acgccccatt catctctcgc atgatttttt
    29581 agcggaggtt gtggataagt ctagtgtggt tgttgatgcc acgatgggta atggaaatga
    29641 tactgccttt ttggcacaat tagctaaaaa ggtttatgct tttgatgtgc aagaacaagc
    29701 tattagaaaa acaagtgagc ggttagcgca gctaggatta ttgaacgctg agctgatttt
    29761 agcgggtcat gaagcggttg atcagtatgt gacagagccg gtgcgcgcag cgatttttaa
    29821 ccttggttat ttgcccagcg cagataaatc tattattacc ttaccaaata ccacactcca
    29881 agctttatca aaactactaa cgttattaat ggtaggtgga cgtattgcta ttatggtcta
    29941 ttatgggcat gatggaggca gtttggaaaa agatgcttta ttagattttg tcaagcaatt
    30001 agaccaaaga aaggtttctg caatgcttta tcagcctctt aatcaggtga ataccccacc
    30061 ttttttaatc atgttggaga aattagcaga cgtttagccc ttatccctgt gtcaagtcta
    30121 ctagaagttc tgcgattatt tcggtggaaa aagaagaaat cccataggct ttggctataa
    30181 atgtgatata atgaagtcac tatattattt ttttaggagt tacattaaca tggaagaccc
    30241 tgtgagtcag cccttagtga ttcaattttt attgttagtt gttttaacct tgttaaatgc
    30301 ttttttttca gccagtgaaa tggccttagt ttctctcaat cgttctcggg tggaacaaaa
    30361 agcagcagac ggtgataaaa aatacgctcg tttgttgcgg gttttagagg aacctaatca
    30421 ttttttatca acgattcaag ttgggattac ctttattagt ttactatcag gagcaagttt
    30481 atcagcttct ttgggtaagg tgatctcagg ttggctaggt aattcagcga ctgcaaggac
    30541 agctggtact atcatctcct tggttttctt gacttatgtc tctattgttt taggagaatt
    30601 gtatccaaaa cggattgcca tgaacctcaa agacaagttg gcgattgttt cagcccctat
    30661 tatcattggg ttagggagac tggttagtcc ctttgtatgg ctcttatcag cttctactaa
    30721 tttactgagc cgacttaccc ctatgacctt tgatgatgca gatgagcaaa tgacacgtga
    30781 tgaaatcgag tatatgttat caaaaagtga ggcaaccctt gatgctgaag aaattgagat
    30841 gttgcaagga gttttctcac ttgatgaaat gatggcgcgt gaagtcatgg tcccaaggac
    30901 cgatgctttc atgattgaca ttaacgatga tccgcttgaa aatattcagg aaatcttaaa
    30961 acaaagtttt tcacgcattc ctgtttatga tgtggataaa gataaaatta tcggtctcat
    31021 ccacactaag cgtctcttgg agtcaggttt ccgccaggga tttgatcaga ttaacatgcg
    31081 aaaaatgtta caagaacctc tttttgttcc cgaaaccatt tttgtagatg atctcttacg
    31141 ccagctgcgc aatacccaaa atcagatggc tattttgcta gatgaatatg gtggtgtggc
    31201 aggacttgtg actttggaag acttgcttga agaaatcgtc ggtgaaatcg atgatgaaac
    31261 cgataaagca gaacaatttg ttcatgagat tggagacaat acctatattg ttgttggtac
    31321 tatgacttta aatgagttta atgactattt tgataccgaa ctagaatcag atgatgtaga
    31381 taccattgct ggtttttatt tgacaggtat cggaaccatt ccaagccagg agcaaaaaga
    31441 agcctacgaa atagataaca aagacaaaca tttagttcta atcaacgata aagtcaaaga
    31501 tggccgtatt acgaaattaa aattaatcct gtctaatata gaacagatta ttgaggaaga
    31561 ctaggctcca tgcctagtct ttttgatgaa aagtgttata atgaaaatgt aaaaacgttt
    31621 acaagtaatg aaggagatgc aatgactgag aaagattatg aacaagtaac agggatggtg
    31681 cattcgacag aaagttttgg ttctgtagat ggtccaggca ttcgttttat cattttcttg
    31741 caaggctgta aattacgttg ccaatattgc cataatcctg atacttggga gatggaaacc
    31801 aacaattcaa aaatcagaac agtcaatgat gtcttgaaag aagcccttca gtacaaacat
    31861 ttctggggaa aaaaaggagg aattaccgta tctggtggtg aagctatgct tcaaattgat
    31921 tttatcactg ccttattcat cgaagctaaa aaattaggta tccataccac actagacacc
    31981 tgtggcttta cctatagacc gacacctgaa taccatcagg ttcttgataa cttattggca
    32041 gtgaccgatt tgattttatt ggacttaaaa gaaattgatg aaaaacaaca taaaattgtc
    32101 acgcgtcagc ctaataaaaa catattgcaa tttgctaggt atttatccga caagcagatt
    32161 cctgtctgga ttcgacatgt cttggttcca ggcttgactg atattgatga tcatttgaca
    32221 cgattgggtg agtttgtcaa aaccttgaaa aatgtcgata aatttgaagt tttgccttac
    32281 cataccatgg gagaattcaa gtggcgtgag ctaggtattc cttatcagtt agaaggtgtt
    32341 aaaccaccaa ctaaggaacg cgttcaaaat gctaaaaatc tgatgcagac cgaatcttac
    32401 acagaatata tgaatcggat tcatcagtca taagagctgt tatagggttg aggtttcctc
    32461 aactttttaa agtattataa gcagatcttt tgtaatcttc ctttgatttt agggggtaat
    32521 tttgctaaaa taaaatgaat aaacaaaatg aggtagaatg attatgtcaa aaattttagt
    32581 ttttggtcat caaaatcctg atacggatgc tattgcatca tcttatgctt ttgattactt
    32641 atcccaaaaa gcgtttggtt tggatactga agtcgtagct ttaggaacac ctaacgaaga
    32701 aacagctttt gcccttgatt attttggtgt tgaggcacca cgtgtggtag agtctgctaa
    32761 agcacaaggg tctgaacaag tgattttgac agaccacaat gaattccaac aatccattgc
    32821 agatatccgt gaggttgagg tatatggtgt ggttgatcac caccgtgtgg ctaattttga
    32881 aactgcaaat cctttataca tgcgtgtgga accagtagga tcagcctcat ctattgttta
    32941 ccgtatgttt aaagaaaatg gtatcgaggt gccaaaagcg attgcaggga tgctcttatc
    33001 tggtttgatt tcagatacct tgttgttgaa atctccaaca actcatgtgt ctgatcacct
    33061 tgtcgctgaa gaattggcag agctagcaga agtgaacttg gaagactatg gaatggctct
    33121 tttaaaagca ggtacaaacc ttgcaagcaa atcagaagta gaattgattg gtattgatgc
    33181 caaaacattt gaattaaatg gcaatgctgt gcgcgtggca caagtgaaca ctgttgacat
    33241 tgcagaagtc ttagaacgtc aagaagctat tgaagcagcc attaaagatg ccatggctgc
    33301 agaaggttat tcagactttg ttttgatgat cacggatatt gttaactcaa actctgaaat
    33361 tctagctatt ggagcaaaca tggacaaggt tgaagcagcc tttaacttca cccttgacaa
    33421 caatcatgcc ttcttagcag gtgccgtttc tcgtaaaaaa caagtggttc cacaattgac
    33481 agaaagtttt ggagcttaat gaaaagacgc tttaaaagga cttggttttt ttgaccaagt
    33541 ccttttttca ctcttctatg ctataataga aggtatgata acaatatttc attcagataa
    33601 actcaccaga caacctttct ttcaagacct tatcaattat ctcgaccagc atgatcacgt
    33661 tatattacga gagatcaaaa aagcctttcc taatgtgaca ggtattgaca aggccatcga
    33721 aagctatgtt caagctggct atattcgccg tgaaaataag cgttatggca tcaatcttcc
    33781 tttggtgagt tctgatcagc agctggcctt agacactatg ctttttgtgg acacctgttc
    33841 agctatgtat gaaaatattt tagcggttgt ttttgagact cagctaacaa accaaaccaa
    33901 tcacgtgatg atcaaggaaa agaccaacat cacgagaagt cagttgactc tagctaatta
    33961 tttttaccgt ctcaaaagag gtgagaagcc atcagctgag cagatggact tgtatgacct
    34021 cttgggggat gtgaatcagg aatatgccct taaatatatg acaacttttt tgcttaaatt
    34081 cacgcgcaaa gaccttgtga tgcaaaaacg tcctgatata tttgtggaag ctctggttac
    34141 acttggttac ctaaaacaag tggagcctac aacataccag ctgctaatga ccttggataa
    34201 agaaagttta acctttatag caccatagaa aagagctagg aacaaggact gttcttagct
    34261 ttttgagtgc ttttattttg tttttgccat taagtaaaga aaataagggg ctccgataag
    34321 cgacactaaa atccctgtct ggatattact gccaacaaga tagactcgtc cgactgtatc
    34381 tgcaacaaga agtaggatca ttccgattaa aatactaatg ggtattgtta ttttatgatt
    34441 actccctacc aagtatgtac taaagtgtcc agctattaag ccgataaaac taatattacc
    34501 aaccaaaaga acactcaaag cacctaagcc agctgctaag acaagtatca aacgacgctt
    34561 acggtttaaa gggagtccta gcccaatagc agtattatca gctaatccca taatattaag
    34621 gaaatgggct tgactataag tcaatagcca aaaacacaat aataaaggag cgatgacact
    34681 cagagtaggc cagtcgtctc ctattaattg gccgctaagc caattgatga ccaaatcgac
    34741 tttgtagcga ttaatatgac caacaagagc aaccattaga cttgaaagta tggtagtaac
    34801 agctacacca gtaagaatta accgtgtagg atcaatttgt ccttgttttg tcaaagatag
    34861 ccaataaacg ctaaaggttg ttacaatagc accaagcatg gcaaataaag gcaagtaatg
    34921 gctgatatga gcttgctcaa agaaagaaaa ggagaccaca atagccaaag agatacctgc
    34981 tcctatagta atacctaata caccagagtc agctaaggga tttcgtgtta aacgttgtaa
    35041 taatagccct gataaagcaa gagaaccgcc tcctagacaa gcagctagaa ttcttggcag
    35101 acgaatgtta ataacgatga aagatatggc atggctagat tttcccaaaa acacatggat
    35161 aaggtccaaa aaagatagat gagattctcc aagggacaaa gctaatccca taagacttac
    35221 aagtagcagg atcaggctag tatataacct taaaaggtgt cggtgagatt tcaatagcgg
    35281 cctcctcttc ttattaacca taaaaagcat ggaaaaccaa ttaagctaac caatgcccca
    35341 agaggggttt cataaggcgg attgagatta cgacaagcaa tatcaaccca taccatgaaa
    35401 ctagcgccag aaactgcaca aagcggcaaa aggtatcggt aatgatgagg tgtaaaatgt
    35461 ttcataagat gaggaatgac taagccgata aaagaaatgc ttccagcaat ggctacagct
    35521 gctgatgata aaataagaac caaaatcata aagactgcac tgatcaagtt ggtcttttgc
    35581 ccaagagctt ttgcctgaga ctcacttaga ctaaggacgg tcaggtgata agataatagc
    35641 tgggctaaac aaagactaag aataataaga ggagcaatat agccgatcat ttgccaattg
    35701 accccgacaa gacctcctgc ttgccagccg ataacagcat ttgtcagatg atagtaattg
    35761 gtaatacctt gacctaatgc tgatagcagt atggaaacca tagctcctgc taagacaagg
    35821 cggagctgat ggtagccttt gccagattga taagaaaggc caaagactaa ggttgctgcc
    35881 aggctagacc ctaacaacga aagcagaata atgagggaat agtgcaagtg aggtacaaac
    35941 gcataagcca gtaccaaggc tagtcctgct ccagcattaa tgcctaaaag gccaggctca
    36001 gcgataggat tacgtgtgat tgcttgcatg atagttccag aaacagctaa cgcagagcca
    36061 gttaaggtag caccaaaaag tctcggtaat cgtatagcta gtaaaacatt ggctttatgg
    36121 ccgttttgct ttccaaataa aatagagtta agatcctgat gtgataaagc aacggctcca
    36181 aagcgcagat gacagtaaat agcaacacat aagctaaaac taagtagcac aaagacaagc
    36241 caaaagatat gtgacttagt gattatctgc gtttttttta agcaaaaacg ctcgagacac
    36301 aaaaagggat gcttagtttt cacttgataa gattgcctct cgtaaagact ctaattgata
    36361 ttctaaagaa agaggatcag taaagtaaaa gacattggca ttgactttga tcacatggtg
    36421 ttttttcaca gctggaatgg attgccatag cttgctttca tatagagcag aaccagtctt
    36481 atcatcttct gcagcaacga cgacataatc accaatatag tctggcaaga cttcctgcga
    36541 caatgacagg taaccttgtt taaaaacttc agtcttcact ttttcaggag catcataatg
    36601 gaaagcttga tggataacct ctccgccacg tccccagtct ttaccaaaaa ggtaaacgtc
    36661 tttttcataa agccccataa tagtaaaggt tgctttgtca cctgtaacgg ccttgacttc
    36721 tttttcataa gctgctgttt tagttttcca atcttttaac catttcttgg ctttgccttc
    36781 tttgttaaag atgcggccga agtcagataa cacttgtaaa taatcacgtt tgcggtattc
    36841 aatcgaaata acaggcgcaa tttctgccaa ttgtttaata ttttcttctg tagagccaac
    36901 aacaatgagg tcaggcttaa gcgttgtaat ggcttctaaa tcagttgcag taacttgctt
    36961 tgcttttttt acagtctttg ctaagatggg gtttttcttg tcataagaag taaccccgac
    37021 tagattcata tctaattttt tgaggtaacc agtgtaagtt gaagccagac tgactactcg
    37081 cttaggctgt ttaggaatat ctccgtaata agtcacacca gcaatttgag gcataggcga
    37141 taaacttttt gaggcggtgt tagaatgatt agttgcttga ttaccacagg cgattaaggt
    37201 aatagttgtc aaacagagtg ttaatagtag agttaatttt ttcatgtatg ctaattctcc
    37261 tagaataaag ttattgaagc tgataagtca gcactatagg acagttgtgg ataggatcat
    37321 caaccagtac cggtttgatt tggaagatgt cttgaatgat aggtgaagtc ataacatctg
    37381 ctatagtacc agtataatgg atcttgccat gtttcatagc aatcagatga tccgaatagc
    37441 gggctgaaag attgaggtcg tgaagaacca taacaatagt cttgccggca tccttattta
    37501 gggatttaac gagctctagg atttctagtt gatgatttaa atctaaataa gtagttggct
    37561 catctaaaaa aatagtatca gtgccttggg ctaatgccat tgcaagccaa accctttgac
    37621 gttgcccccc agaaagagca tcaacaggtt gatcagcata ggccatgaca ttggtggctt
    37681 gcatagccca atgaatggct tttttgtcag ctggactaag atttccaaag taagattgat
    37741 ggggaaaacg tccgtaagaa accaattcgt aaacagttat gccgttagtt gcttcttgaa
    37801 cttgaggtaa aagagctagt tttttagcaa cctctttagt ttctaatgtg gcaatgtttt
    37861 gcccatttaa atacacaact ccctgttttg gtggtaataa tcttgtcagt gcttttaata
    37921 aactagactt cccacagcca ttggcgccaa taatggtcgt aattttacct tcaggaatat
    37981 aaaacgataa tttatcgatg atggtacgct gttcataggc gattgtgagg tcttcagcac
    38041 taattgttgt catatggttg cctcctttct aaaaagagtc aaagggttga tgaaagtgca
    38101 cattaaagta ttcatctgcc cattttaaaa tatttgagaa gtagcttatt ttctcaaaat
    38161 cttcactatt atagcataga aaaaaggctt aacaaagcct tttttgatgt gatttttagg
    38221 atgtattaaa aaagaaatat tagtttagaa tgattaaaaa caatagcgag aatcattcta
    38281 aactaacgtc aacatactct agaggtagtg tttggcgatt gctaaatcac cagcataagc
    38341 tacttgttgt cctttaacaa tttgataagc atctgcgcct ttcccagcga taatcacagc
    38401 atcttttgct ccttggcaaa gagacatggc tttttgaata gcttgctcac ggtcactaat
    38461 aatctctacc gggcgtgcga tatggctggc aatttctttt gaaatatctt ctggatcctc
    38521 gaaattaggg tcatcagcag tgagaatgac ggtgaggttt ggatgctgat gaatcaccct
    38581 accaaaatca gctcgtctgc tctcgccttt atttcctggt gcacctaaaa tcaacattaa
    38641 tttgcctgtc tgatgttctt ccacaacgct gagtaatttt tctaagctgt caccattatg
    38701 ggcataatca acaaaaactt tagcatgatt tgtcatagtg aggacttcca tacggcctgg
    38761 gacgcgagtc ttagcgatgc ctttttgaat atcagctagg ctagcaccca aacgaaggca
    38821 ggcaagtcca gctgccatag cattttcttg gttaaaatgg ccaattaatt gaatgtcata
    38881 atggccagct aattgtcctt tagcttcaaa ggagaaagct tggctagtgg tgatctggtt
    38941 gtcagacaag ggaccataaa acacatgctc ttgatccgct acctgatcag ctagaaatga
    39001 aaaatgatcc atcccgctat tgatcacaac agctctgcta ttctccatta aaagacgctt
    39061 atgatagaaa taatcttcaa acgttggatg ctcgattggg ccaatatgat caggactaat
    39121 atttaggaaa accccaacat cgaacgtaag cccatagaca cggtcaacga ggtaagcctg
    39181 actagaaact tccataatca agtgggtcat gccattagtc acacactcag ccatcatagc
    39241 aaaaagatcc aagctttcag gagttgtaag ttgtgatttg aaaaaggtct tgccatctaa
    39301 ggtggtattc atggtagaaa acatggcagg tttataggat tctttcaaca tgtggtaggc
    39361 aaaataagca gccgtcgtct ttcccttagt acctgtaaaa gctagtagtt tgagcttttc
    39421 ttgagggttg ccatagaaag ccatggcaat tagactcata gcttttttga tatcagtgac
    39481 caaaacaacg ggaataccaa gctcgtaatc tacctctgaa atatagagtt gtaatccgtt
    39541 agtgattgct tctttaaggt agtcagcttt gaaagttgct cctttagcaa aaaaaagggt
    39601 cttgccatct acttgacggc tatcgtaact caagcgctca aaacttaagc cttgataatg
    39661 gtagtggtat ccgtcggcat caagaacctc acgaaaatta tggtcttttt ttaaaatgtc
    39721 taataattgt tcaatggtta tcataccctt attttatcct taaagttctt gttttacaag
    39781 tcacaagcaa aagtttataa taaaactaat agaaactagg aaagaataga tatgtcgaca
    39841 gaaaaaaaac aactgacaca agaagaatta atggttcagg gagcagcctg gtccacagct
    39901 ggtaatttca ttagccgact tttgggagtg ttatacatta ttccctggta tatttggatg
    39961 gggcaatacg cgattcaagc caacgctctt ttcaatatgg ggtataatgt ttatgcttat
    40021 tttttattga tttcaaccac aggactcaat gttgcgattg ctaagcaggt tgccaagtac
    40081 aattccatgg gacaaactga gcatagctat caattaatcc gaagtaccct aaaactcatg
    40141 cttggtctgg gccttatttt ttcagtcatt atgtatctgg gctctccttt atttgcgagc
    40201 ttatctggtg gtgatgacac acttgttccc attatgcaca gcctttcttt agcagttttt
    40261 atttttcctg taatgagtgt cattcgaggt atctttcaag gccacaataa tatcaagcct
    40321 tatgcgatta gtcagattgc tgagcaactc attcgggtta tctggatgct tttgaccacc
    40381 ttccttatca tgaaattagg ttctggtgat tacgcttcag cagtgactca gtcaactttt
    40441 gcagccttta tcgggatggt ggctagtatg ggtgttctgg ggtactatct ttggaaacag
    40501 ggacttctag cagctatttt tagtaagcca gatcatactg tttccattga tatcaagggc
    40561 ttgttgcttg aaacattaaa agagtctatt ccgtttatcg taacaggaag tgccattcaa
    40621 gcctttcaat tgattgatca atggactttt gtcaatacta tgactctttt tacagattat
    40681 agccgttctc aattgcttgt tttatttggt tactttaacg caaacccagc caaaattaca
    40741 atggttttaa ttgcagtagc agcatctatt ggtggtgtag gcattgcctt attaactgaa
    40801 aattatgtca aaaaagacat gaaagcagct gctcgcttaa tcattaacaa cattgaaatg
    40861 ttagtgatgt ttttgttacc cgctcttact ggggcaatta ttttagcaag acctctatat
    40921 tctgtttttt acggagctag cgaggatcgt gccattcacc tctttgtggc ggtcctcttc
    40981 caaaccttgt tgatggcgct ttacaccctt ttttcaccga tgcttcaagc tctttttgaa
    41041 aatcgaaaag cgatttacta ctttgcctat ggtatcttga ttaagttagt tttacagata
    41101 ccgcttattt atttgctaca tgcttatggt cctttactag cgacgacgat tgctttagtg
    41161 gtgccgattt atttgatgta tcgacgccta tatcaggtta ctcattttaa ccgcaaactg
    41221 ttgcaaaaac gtttattatt aaccttgatt gaaaccttat taatgggact ggtcgtgttt
    41281 gtggccaact ggctattggg ctatgccttt aaaccgacag gccgcttgac cagccttctt
    41341 tatctcctca ttattggtgg cgtgggaatg acggtttaca cggcactcac cttgctgacg
    41401 catcaactgg ataaattaat tggtagcaaa gctagtcgcc ttcgtcagaa attaggctgg
    41461 cattaatata atgacaccaa gaacgatttc gtgagtgttt atcgaaatcg tttttttctt
    41521 aatgatctcg cttagtaaag gtagctgata aaaaatcagg atttcccaag caaactactg
    41581 atatttttgg tataatgata aaagtgtaaa aaaatagaag gaagtatatc aacatgggaa
    41641 aatgtcaagt tatttcgcat ccgcttatcc aacacaaact ttcaatctta cgtcgtcaaa
    41701 ccacatcaac taaagatttt cgtgagttgg ttaatgaaat tgcgatgctg atgggatatg
    41761 aagtgtcacg cgaccttccc ttagaagatg tcgacatcca aacgccagtc tcaaaaacgg
    41821 tccaaaaaca gttagctggt aaaaaattag ctattgttcc gattcttcgt gctggtattg
    41881 ggatggttga tggcttgtta agtttggtac cagctgcaaa agttggccat atcggcatgt
    41941 accgtaatga agaaaccctt gaacctgttg aatacctcgt taaactacca gaagatatta
    42001 accaacgaca aattttcctt gttgatccaa tgttggcaac aggtggttcg gctatcttag
    42061 cagtggattc gttgaaaaaa cgtggcgcag caaatattaa gtttgtctgt ttagttgcag
    42121 caccagaagg ggtgaaaaaa cttcaagaag ctcatccaga tattgatatc ttcacagcag
    42181 cacttgatga tcatttaaat gaacatggct atatcgtgcc aggcttgggc gatgctggtg
    42241 atcgtttatt tgggacaaaa taaaagagac aaccatcaaa tagtagcctt tttaggccaa
    42301 tcgttcttga ttttgttaaa aaaatcagag agatttctct tctttaaagg aaaagcaagt
    42361 ttatgatttg acctttattg accaaaatat tataataagg acaatcagta aaaaagagat
    42421 gacctagtgg gttgccgtct ctcacaatga cgctactaaa aacccttgta gattggagaa
    42481 tgtattatga ttcctgttgt tattgaacaa actagccgtg gagaacgttc ttatgacatt
    42541 tattcacgtt tattaaaaga ccgtattatc atgttaacag ggcctgtcga agacaacatg
    42601 gccaactctg tcatagcgca gctcttattc cttgacgcgc aagacaacac taaagatatc
    42661 tacctatatg tcaacacacc aggtggctct gtctcagcag gcttggctat tgtcgatacc
    42721 atgaacttta tcaaggccga tgtgcaaact attgtcatgg gtatggcagc ttctatgggg
    42781 acagtcattg cttcatcagg aacaaaaggc aaacgcttta tgttaccaaa tgcagagtat
    42841 atgatccacc agccaatggg tggcacaggc ggtggcacgc aacaaacgga tatggctatc
    42901 gcggcagaac atctcttaaa aacacgtcac cgtttagaaa aaatcttagc gcaaaatgct
    42961 ggaaaaacga ttaaacaaat ccataaagat gctgagcgtg attattggat gagtgctgaa
    43021 gaaaccttgg cttacggttt cattgatgaa atcatggaaa acaacgaact aaaataaaga
    43081 ggatctctgc aaaagtattc tagaaacttc taggatgctt tttctcttag aaaggaaaat
    43141 aatgatacag cctactgcat taaaacagct tcaagagtta aaacgtcagt tcagatccct
    43201 ttgcaaccaa ttatgagata gtttgaggat gatccttagc agctgtgtgc aagaagttat
    43261 ggcgatttca taaaggatga cctagccagt ctttcttcgt ctcttttact taaagtgtgg
    43321 tataatcaga ttatgctagc cacaactaaa aaaggcattg ccattaaagg tgttgtagat
    43381 cggcttatcc tagatctcat aacaatgctg tagtaccgcc cttaccttgc tactaaagac
    43441 gttgttgaag cacaaattga cttgcaaagg atcttgactg ataggtacat gtccggtgga
    43501 atcgccaaag cagcaaaaag accaagaccc ttatttggtc tctaccaaaa tataatagaa
    43561 aaggatttag aatgtttcaa aaacaagaac gtattggact cgtcgtttac ctgtattata
    43621 accgagatgc cagaaagtta tcaaaatttg gtgatttata ttaccattcc aagcgctctc
    43681 gttacctcat tatttatatt aataaaaatg atttagacac aaaattagaa gaaatgagac
    43741 gtttgaaatg tgttaaagac attagaccat cagcttttga tgatattgac cgccaatttg
    43801 taggtaacct tcatcgcgac gaaacaaaca accatcaaaa gggttaccaa ccccctagtt
    43861 actgattagc gtttcagatt tagttagctt attcaagtaa gaaggttttg acagatgctc
    43921 acactacttc aagacaatga gacaactaga gcattagtga ataactgagt tgactgtcac
    43981 ttctttaata agaacggata ggattggtcc taaaatagag cactgcaa
//
LOCUS       DAUDYX010000009        43430 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.009, whole genome shotgun sequence.
ACCESSION   DAUDYX010000009 DAUDYX010000000
VERSION     DAUDYX010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43430
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.009"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(107..1243)
                     /gene="dnaJ"
                     /locus_tag="VHD02_000487"
     CDS             complement(107..1243)
                     /gene="dnaJ"
                     /locus_tag="VHD02_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEQ5497120.1"
                     /translation="MNNTEYYDRLGVSKDASQDDIKKAYRKMSKKYHPDINKEAGAEQ
                     KYKDVQEAYETLSDSQKRAAYDQYGAAGAQGGFGGGAGGFGGFDGGGFGGFEDIFSSF
                     FGGGGSRNPNAPRQGDDLQYRVNLSFEEAVFGVEKEVSYNREATCGTCLGSGAKPGTA
                     PVTCRKCHGSGVMTIDTQTPLGMMRRQVTCDICHGSGKEIKEPCQTCHGTSHEKQAHK
                     VSVKIPAGVETGQQIRLQGQGEAGFNGGPYGDLFVILNVLPSKQFERNGSTIYYSLDI
                     SFTQAALGDTVEIPTVHGDVEMAIPAGTQTGKTFRLKGKGAPKLRGGGQGDQHVTVNI
                     VTPTKLNDAQREALQAFAEASGEKMLHPKKKGFFDKVKDALEDI"
     gene            complement(1524..3350)
                     /gene="dnaK"
                     /locus_tag="VHD02_000488"
     CDS             complement(1524..3350)
                     /gene="dnaK"
                     /locus_tag="VHD02_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEQ5497121.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVAEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDARLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIKSNDGLSEEEIDRMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKAAQESGNLDDMKAKLEALNEKAQ
                     ALAVKMYEQAAAAQQAAQGAEGAQANDSANNDDVVDGEFTEK"
     gene            complement(3531..4103)
                     /gene="grpE"
                     /locus_tag="VHD02_000489"
     CDS             complement(3531..4103)
                     /gene="grpE"
                     /locus_tag="VHD02_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEQ5497122.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEETP
                     EKSELELVNERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            complement(4106..5140)
                     /gene="hrcA"
                     /locus_tag="VHD02_000490"
     CDS             complement(4106..5140)
                     /gene="hrcA"
                     /locus_tag="VHD02_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEQ5497123.1"
                     /translation="MITQRQNDILNLIVELFTQTHEPVGSKALQRTIDSSSATIRNDM
                     AKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQDIYHVIKAFDFEAFKLE
                     DMLQKASHILAEMTGYTSVILDVEPARQRLTGFDVVQLSNHDALAVMTLDESKPVTVQ
                     FAIPRNFLTRDLIAFKAIVEERLLDNSVIDIHYKLRTEIPQIVQKYFVTTDNVLQLFD
                     YVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLRQSLKEGEMASVQVADS
                     QEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLVNIIGKVLAAKLGDYYR
                     YLNSNHYEVH"
     gene            complement(5275..5844)
                     /locus_tag="VHD02_000491"
     CDS             complement(5275..5844)
                     /locus_tag="VHD02_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEQ5497124.1"
                     /translation="MRKRLKFPYFLTLLACFLLLIVCPLLSSQRIASADKEVRVNYSQ
                     KQFITKMGKEVKPLAKYYGIRPSILIAQILLETHDGKTLLASKYHNLFSKKATPGQAA
                     ITLKSPKQTNQNVRYAIYKDDASAIRDYLRMLRQGKEVDKRLYRNLATEKGYKAPAKS
                     LQKYLHYTDKTYARRLIQVIESNDLTNYD"
     gene            complement(5841..6584)
                     /locus_tag="VHD02_000492"
     CDS             complement(5841..6584)
                     /locus_tag="VHD02_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEQ5497125.1"
                     /translation="MKYNSLNYLVQLLIVIVFLGGLYLFIKPEESVTPTQLNKKEIQQ
                     KDIKKTDRLRALPKVSVEDWELVLVNRDHITKEMSPELADINGISVDKRIEQATSDFL
                     AAAQAIDSQEHLISGYRSVDYQTELYQSYIKKEMANDPTLTQEAAEALVQTYSQPPGT
                     SEHHTGLAIDMSTVDTLNASDPSVAKAVQKIAPDYGFVLRFPEGKKTSTGVDYEDWHY
                     RYVGKASARYMAQHNLTLEEYIAALKEKR"
     gene            complement(6574..7281)
                     /locus_tag="VHD02_000493"
     CDS             complement(6574..7281)
                     /locus_tag="VHD02_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEQ5497126.1"
                     /translation="MTKTRLYIARHGKTMFNTIGRAQGWSDTPLTKKGEEGIRELGLG
                     LKDATIPFKAAFSSDSGRTMQTIEIILRESENEFLPYTKDNRIREWCFGSLEGTYDSE
                     LFLGVLPRTKAFENRDNLRDVPYSELAESIVEIDTANWAEPWEVLRKRIWEGFEAIAL
                     SIQNAGGGNALVVSHGMTIGTFLWLIDPDRDKQYIDNGSVTVVEFDDGQFTIKTIGDM
                     SYRYRGREIIEERSDEI"
     gene            complement(7673..7912)
                     /locus_tag="VHD02_000494"
     CDS             complement(7673..7912)
                     /locus_tag="VHD02_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEQ5497127.1"
                     /translation="MIWSLIVGAFIGMIAGKMTEKSQSMGCFTNIIAGLIGSAVGQRL
                     FGDWGIQLAGMAIFPSILGAAIVIVLVSAIFGKRY"
     gene            complement(8131..8346)
                     /locus_tag="VHD02_000495"
     CDS             complement(8131..8346)
                     /locus_tag="VHD02_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497128.1"
                     /translation="MIVADSNKLKYFVSEKKAVGLSQAAFLYVMLVTVYKKTIIYAKI
                     RLTGIALEPSGVSDGYLMNHHVCLHYY"
     gene            complement(8343..9782)
                     /gene="gatB"
                     /locus_tag="VHD02_000496"
     CDS             complement(8343..9782)
                     /gene="gatB"
                     /locus_tag="VHD02_000496"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEQ5497129.1"
                     /translation="MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHKEMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIKIKLEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEQFGTKTELKNLNS
                     FSNVRKGLEFEVERQAKLLRSGGVIRQETRRYDEANKGTILMRVKEGAADYRYFPEPD
                     LPLYEIDDAWIDEMRAQLPQFPAQRRAKYEEELGLSAYDASQLTATKVLSDYFETAVS
                     LGGDAKQVSNWLQGEVAQFLNAEGKTIEEIALTPENLVEMIAIIADGTISSKMAKKVF
                     VHLAKNGGSARAYVEKAGLVQISDPAVLVPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVAQQLLAQELQKLRD"
     gene            complement(9782..11248)
                     /gene="gatA"
                     /locus_tag="VHD02_000497"
     CDS             complement(9782..11248)
                     /gene="gatA"
                     /locus_tag="VHD02_000497"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEQ5497130.1"
                     /translation="MSFNHKTIEELHDLLVAKEISATELTQKTLEDIKSREEAVGSFI
                     TVSEEAALKQAAAIDAKGIDADNLMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATSVANAYAKDMIVIGKTNMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTV
                     KENAQLLNVIASSDVKDATSAPVRIADYTSKIGRDIKGMKIAFPKEYLGEGIDPKIKE
                     TVLAAAKQFEALGATVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGFRADDA
                     KNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIEDFDKVFA
                     DYDLILGPTTPAVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFVDGLPVG
                     LQLIGPKYAEETIYQAAAAFEAVTDYHKQQPIIFGGDK"
     gene            complement(11248..11550)
                     /gene="gatC"
                     /locus_tag="VHD02_000498"
     CDS             complement(11248..11550)
                     /gene="gatC"
                     /locus_tag="VHD02_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEQ5497131.1"
                     /translation="MKISEEEVRHVAKLSKLSFSESETTTFATTLSKIVDMVELLNEV
                     DTEGVAITTTMADKKNVMRQDVAEEGTDRALLFKNVPEKENHFIKVPAILDDGGDA"
     gene            complement(11782..12101)
                     /locus_tag="VHD02_000499"
                     /pseudo
     CDS             complement(11782..12101)
                     /locus_tag="VHD02_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dikinase"
     gene            complement(12545..13099)
                     /locus_tag="VHD02_000500"
     CDS             complement(12545..13099)
                     /locus_tag="VHD02_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEQ5497132.1"
                     /translation="MRALISIDYTNDFVADDGKLSAGKSAQAIATKIAEVTKTAFDQG
                     DYIFFAIDCHDQNDPWHPESKLFAAHNIKGTTGRHLYGPLAEVYSYMKQHPRVFWIDK
                     RYYSAFSGTDLDIRLRERGITQLVLTGVLSDICVLHTAIDAYHLGYQLEIVKSAVASL
                     TKESYEWSLAHFEQVLGAKLINDY"
     gene            complement(13246..14028)
                     /gene="codY"
                     /locus_tag="VHD02_000501"
     CDS             complement(13246..14028)
                     /gene="codY"
                     /locus_tag="VHD02_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEQ5497133.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLETELPYNTMASRLADIIDCNAC
                     IINGGGTLLGYAMKYKTNTDRVEEFFEAKQFPDTYVKAASRVYDTEANLSVENELTIF
                     PVESKDTYPGGLTTIAPIYGGGMRLGSLIIWRNDNEFSDDDLILVEISSTVVGIQLLN
                     LQTENLEDTIRKQTAVNMAINTLSYSEMKVVAAILGELDGNEGRLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFAKLKEF"
     gene            complement(14246..15460)
                     /locus_tag="VHD02_000502"
     CDS             complement(14246..15460)
                     /locus_tag="VHD02_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEQ5497134.1"
                     /translation="MKIIEKSSKLEHVAYDIRGPVLDEANRMIASGEKILRLNTGNPA
                     AFGFEAPDEVIRDLIVNARLSEGYSDSKGIFSARKAIMQYCQLKGFPDVDIEDIYLGN
                     GVSELISMSLQALLDNGDEVLVPMPDYPLWTACVSLGGGKAVHYLCDEEAGWYPDIAD
                     IKSKITSRTKAIVVINPNNPTGALYPKEILEDIVALAREHQLIIFADEIYDRLVMDSK
                     EHIAIASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKDHVKGYIEGLNMLANMRL
                     CSNVLAQQVVQTSLGGHQSVDALLLPGGRIFEQRNFIYEAINAIPGLSAVKPEAGLYL
                     FPKIDRQMYRIDDDEEFVLQLLKQEKVMLVHGRGFNWKDPDHFRIVYLPSVEELEDVQ
                     EKITRVLYQYRR"
     gene            15691..16143
                     /locus_tag="VHD02_000503"
     CDS             15691..16143
                     /locus_tag="VHD02_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015055992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEQ5497135.1"
                     /translation="MSLKYKRILVAIDGSYESELAFNKGVNVALRNDATLLLVHVIDT
                     RALQSVATFDTYIYEKLEQEAKDVLDDFEKQAQIAGITNTKQIIEFGNPKNLLAHDIP
                     DRENADLIMVGATGLNTFERLLIGSSSEYIMRHAKIDLLVVRDSTKTL"
     gene            complement(16266..17654)
                     /locus_tag="VHD02_000504"
     CDS             complement(16266..17654)
                     /locus_tag="VHD02_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ5497136.1"
                     /translation="MTVKAVFFDIDGTLLNDRKNIQKTTQKAIQQLKKQGIMVGLATG
                     RGPGFVQPFLENFGLDFAVTYNGQYILTRDKVLYQNQLPKSMIYKVIRYANEKKREIS
                     LGTASGLAGSRIIDMGTSPFGQVISSFVPKSWARTVEGSFKHLIRRIKPQSFRNLVTI
                     MREPIYQVVLVASQAETKKIQEKFPHIKITRSSPYSLDLISVDQSKIKGIERLGEMFG
                     FDLSEVMAFGDSDNDLEMLSGVGIGIAMGNAETVVKDGAHFTTDSNNNDGISKALAHY
                     GLIHFDVEKSFKSRDENFNKVKDFHRLMDSDTIETPRSYTISEAGYRSGFKVEEIVEF
                     LYAASKGDQQQFTQAIFDLHGAVDQAVNKVQAKKHVETPLIGQVDALADLLYFTYGSF
                     VLMGVDPQPIFEAVHEANMAKIFPDGKAHFDPVTHKIQKPDYWQERHAPEVAIKKELD
                     KQLQKSLQRFDK"
     gene            17726..18691
                     /locus_tag="VHD02_000505"
     CDS             17726..18691
                     /locus_tag="VHD02_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEQ5497137.1"
                     /translation="MKKILVLHTGGTISMQADNSGRVVPNQDNPMTKIHAAAQDIQLT
                     VSDFLNLPSPHITPHHMLSIYHHIQERTDVFDGIVITHGTDTLEETAYFLDTMALPTN
                     IPVVLTGAMRSSNEVGSDGIYNYLTALRVASSDKAKEKGVLVVMNDEIHAAKYVTKTH
                     TTNISTFQTPTHGPLGIIMKNDLLFFKTAEPRIRFDLRCISGTIPIIKAYAGMGDGSI
                     LSLLTPGSIQGLVIEALGAGNVPPLAVGEIEHLITLGIPVVLVSRCFNGMAEPVYAYE
                     GGGAMLQEAGVMFVKELNAPKARLKLLIALNAGLTGQELKDYIEG"
     gene            complement(19040..19744)
                     /locus_tag="VHD02_000506"
     CDS             complement(19040..19744)
                     /locus_tag="VHD02_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ5497138.1"
                     /translation="MISYLKADLFRIRQERLALFSCMLLVVIAVGVAYSFRKTPNQVI
                     MIQIINIITDFVTLFFLTPARIFFSDDYNYRTINNVVVKQQNRTLIIIYKLLVSFGLS
                     LLFISLTYLLSALAFHLFTGQAYYGIVIDRMIKQLPFYIAIIALCQFLFNILRKTYQS
                     ITAYILYVLLFDKVSATLANHFFKLDISPVLMFKHLSLSLGDKHLSSTSILVAFGLTL
                     CYLLGNVFLFSKRELK"
     gene            complement(19757..20659)
                     /locus_tag="VHD02_000507"
     CDS             complement(19757..20659)
                     /locus_tag="VHD02_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ5497139.1"
                     /translation="MTQQSMLLSIEQVNKSYGKNQVLSDISFDIYKGEICGLVGQNGA
                     GKTTLMRILSGLIGKDSGQIKQLQPYRMGSIIESPTLYPNMTAHDNLYYAALQLRLAD
                     AKERIYEVLELIGLEKVSKKKKVKDYSLGMRQRLAIGLSILDFPEFLILDEPINGLDP
                     AGIKEMRQIILNLRDCYGITILISSHILSELDLVVDRYVIMHKGKVIKSMDKAELKAQ
                     VKVQIALHTSDDQLVKNRLLELGLRVETDGQMLLIKPTLSVMELIKIVLDLPVEIFDI
                     YHHQVSFEHYYLDLLGKDAASPLI"
     gene            complement(20952..22967)
                     /gene="recG"
                     /locus_tag="VHD02_000508"
     CDS             complement(20952..22967)
                     /gene="recG"
                     /locus_tag="VHD02_000508"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEQ5497140.1"
                     /translation="MILTAPMSNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVAQGISQSTLIKAIKSAFE
                     IDAHLELKENLPATLLEKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKAENKSETNGLPILYSKHAMETKISSLPFILTNAQKRSLDDILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSVIDELPAGRKPIMTRWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDARMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     YKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARKVSAAIVSDPNWIYEKQWQLVAQNIRKKEVYD"
     gene            complement(23342..24796)
                     /locus_tag="VHD02_000509"
     CDS             complement(23342..24796)
                     /locus_tag="VHD02_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5497141.1"
                     /translation="MERLGGADLDYCYHNHHDTSMISAEQLVFTYHDQKNPACQVPTC
                     QIASGQFIVLCGPSGSGKSTFLKLLNGIIPDYYAGKYEGRLDVADCQAGRDSVETFSR
                     SVASVFQNPASQFFYREVQHELVFPCENQGLDAKVIMKRLWTLAEDFAFAELLNKDMF
                     GLSGGQKQRVAIATAIMQGTNIMLFDEPTANLDSAGIAAVKAYLTQLKAAGKTIIVAE
                     HRLHYLMDLADNFFYFKNGRLTDKLTTQDLLALTDEQRQDMGLRRLDLSDLKPVLAGK
                     IESQHYRPDDSLCIEHLTVRAGSKILRCIEQLSFAAGSISGITGSNGLGKSQLVYYIA
                     GILDDKKATIKFQGIPLSAKQRLSKTSIVLQEVSLQLFAESVSKEVNLGHERHPRTTE
                     VIERLSLTTLLERHPASLSGGEQQRVMIAASLLADKDILIFDEPSSGLDLLQMKALAN
                     LLMQLKTQHKVVILISHDEELLSSVCDTIYQMLP"
     gene            complement(24733..25413)
                     /locus_tag="VHD02_000510"
     CDS             complement(24733..25413)
                     /locus_tag="VHD02_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEQ5497142.1"
                     /translation="MRLDVRTKLLLLVLANACFFFRVDGFLEFIIVIFLLLLLSALNK
                     KKLAFKLAVVYLLMIGLSVIPLSIFPSYLDHLLSFVSIAGRLVFPSLLAGLITIKTTT
                     IYELVHGLRKWHFPEVWLLTLAVMCRFIPMIRQECCVIHRSLTIRGIILTKWSILIRP
                     KQYLEYLMVPLLLSLIRSSQELTIASLTKGLAVNKGTSECFSSHLTWKDWGVQIWITV
                     IIIITILQ"
     gene            complement(25410..26003)
                     /locus_tag="VHD02_000511"
     CDS             complement(25410..26003)
                     /locus_tag="VHD02_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEQ5497143.1"
                     /translation="MKQLTIKDIMITGAFAALYFICVGLGTFINIFFDRSGNMLYAPA
                     FAALLGGTIYFLLIAKLQTFGPITLLGLMMGGFFFLSGHFFAAMFPSIIFGLIADIIA
                     SLGKYKSRFFNDLSYMVFAFVNSGPILLMWLLKDAYVESLLARGKSMTYVNRIMYDFS
                     LGHVIWFVGIVLAGALLGSAFAHYLLKKHFIKSGLLS"
     gene            complement(26000..27670)
                     /locus_tag="VHD02_000512"
     CDS             complement(26000..27670)
                     /locus_tag="VHD02_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5497144.1"
                     /translation="MAKPNRSTLSLVWRLLTLMKTLLPWIILAVGFAVMGFVITVSIP
                     TGIAYLGLLAIRQEVIPILALYLLIALAFLRGFVRYGEHYFGHFVAFHSLAAFRNLIF
                     KKLRALSPACLDSQDSGYLLKMIGEDIEALEVFFAHTIAPICTAILSAGLMFWYFCQS
                     SWQLALLALATYACLAIVIPIYFANILQVLLKSQNEGRKDYLSYFLESLRSVKDLLQF
                     QVLDEQFERLIKKSNHVNALDRNVAQAQFLQMALTFFWLGLMILAFSYMVFDGICHDS
                     LSFDKGLLTFIAFTASFSPFLELGRLPLGFKRAMNAARNIFDLLDENVIVDEGTKQID
                     RLRSVSFEDLSFAYPKRQELVFKDLSVTFQEKGIIGIKGESGSGKSTLVKLIMKWYNW
                     KTGDIFLNDRNSCLLNAAKLQDTIAYVPQTAQLFQQSIRENLTFGRQDISDESIWNLA
                     EACGMKDRLLACKEGLDTIIKSPSDFSAGEGQRLELMRALLKDASCYIFDEPTSNLDS
                     LNEAILLDLIKTHCQGLVFLISHRPSTLACADHLFCVKNGFLKEVNKK"
     gene            complement(27663..29426)
                     /locus_tag="VHD02_000513"
     CDS             complement(27663..29426)
                     /locus_tag="VHD02_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEQ5497145.1"
                     /translation="MMETEDTISRGKRKRLLKRLRERIAPKRYLLYVSAFLSWLQFVM
                     RMISFYLIAKTFSTFILGHAIALGRLAGLLLLLNVVGFVLAILGKQLQGIASQFARDS
                     LKQSFFEAFIDLDGQFDAHASDADILTLASQGIDSLDTYYGYYLSLSMRTKWNCTTIM
                     ILVFLIYPLAGLVFLGVLPLIPLSIVAMQKRSKQIMSHYWSSYMDVGNLFMDDLKGLN
                     TLYSYQATERYEQEFSGKAEQFRKATMSLLGFQLQAVGYMDAVMYLGIGLSGFLAVQA
                     LATGQLSFFNFLFFLLIATEFFTPIREQGYGMHLVMMNTKMADRIFSFLDSVPARKDN
                     KSKTAINFNQIDIQNISLAYEKKTVLSGVTMTLTKGQLTAIAGVSGQGKTSLAQLLLK
                     RQSATTGHILFDGLDSDNLSQEIINQQVLYVSDQSTLLNRSIYDNLRLAANLSKKEIL
                     DWIDQHGLLSFINWLPDGLDTIVGENGKLLSPGQKQQVICVRALLSKRSLYIFDEATS
                     SLDAENERIIDNLITRLAKTAIVIVITHKMSRLKGANQVLFLSTGQPACLGKPCDLYR
                     DQPTYRHLVDTQARLEASIYG"
     gene            complement(29423..30259)
                     /locus_tag="VHD02_000514"
     CDS             complement(29423..30259)
                     /locus_tag="VHD02_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5497146.1"
                     /translation="MSKNSSHIQVQNLSFAYEEKLVLDDLTFSIPKGKITTIMGANGS
                     GKSTLLQLLTKNLPLKQGQVWLEQEELATISLKAFAKKVAVVHQYHQVVEGLLVGELV
                     AMARLARRSFFKTTTKEDEERLAWALEVTALSDYVYRDIQHLSGGEQQRVWIAMALAQ
                     DTDIIFLDEPTTYLDIKYQLDILKLITKINQELGLTIVLVLHDINQALSLSDHLIGLK
                     DGRLYAQGQPRDLLDQQFIRDVFGVELPFVERNGQQFVLTSLVNETNDIRTQRRDRNK
                     NR"
     gene            complement(30256..31278)
                     /locus_tag="VHD02_000515"
     CDS             complement(30256..31278)
                     /locus_tag="VHD02_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEQ5497147.1"
                     /translation="MTASDLQRDQSLLAKKRFRSGLYLVLLILSLLFLAVIALSLGGL
                     AVSYGAIVKGLFVTYDPQVALIYDLRFPRIVIALLAGAGIAVSGVLFQAVLKNPISDP
                     AITGICSGASFMVLVSSLLLPQLLLYGPIVSFLGGGVSFLLIYGLAWKKGLNPIRLIL
                     TGIAINALFMGLSTALTSFFTSASPMVNALLAGHISQKTWADVGVLFPYTFIGLLLAL
                     LLSKTCNLLLLDDQVIRHLGIDATALRLGISLVAVLLASVATSIVGVVSFLGLIVPHM
                     SRLLVGSKHQILIPFSALLGAFVFLLADTLGRSLAYPLEISPAIIMSIVGGPYFIYLL
                     RRSDII"
     gene            complement(31280..32164)
                     /gene="isdE"
                     /locus_tag="VHD02_000516"
     CDS             complement(31280..32164)
                     /gene="isdE"
                     /locus_tag="VHD02_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HEQ5497148.1"
                     /translation="MIKRCKGIGLALMAFFLVACVNQHPKTAKETEQQRIVATSVAVV
                     DICDRLNLDLVGVCDSKLYTLPKRYDAVKRVGLPMNPDIELIASLKPTWILSPNSLQE
                     DLEPKYQKLDTEYGFLNLRSVEGMYQSIDDLGNLFQRQQEAKELRQQYQDYYRAFQAK
                     RKGKKKPKVLILMGLPGSYLVATNQSYVGNLLDLAGGENVYQSDEKEFLSVNPEDMLA
                     KEPDLILRTAHAIPDKVKVMFDKEFAENDIWKHFTAVKEGKVYDLDNTLFGMSAKLNY
                     PEALDTLTQLFDHVGDHP"
     gene            complement(32161..33024)
                     /locus_tag="VHD02_000517"
     CDS             complement(32161..33024)
                     /locus_tag="VHD02_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEQ5497149.1"
                     /translation="MTKVVIKQLLQVIVVFMISLSTMTGVVYADKGQIYGCIIQRNYR
                     HPISGQIEDSGGEHSFDIGQGMVEGTVYSDAMLEVSDAGKIVLTFRMSLADYSGNYQF
                     WIQPGGTGSFQAVDYNITQKGTDTNGTTLDIAISLPTVNSIIRGSMFVEPMGREVVFY
                     LSASELIQKYSGNMLAQLVTETDNSQNQEVKDSQKPVDTKLGESQDESHTGAMITQNK
                     PKANSSNNKSLSDKKILPSKMGLTTSLELKKEDKFRSKKDLSIMIYYFPTFFLMLGGF
                     AVWVWKKREKK"
     gene            complement(33221..37048)
                     /gene="shr"
                     /locus_tag="VHD02_000518"
     CDS             complement(33221..37048)
                     /gene="shr"
                     /locus_tag="VHD02_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein Shr"
                     /protein_id="HEQ5497150.1"
                     /translation="MKKISKCAFVAISALVLIQATQTVKSQEALVQSQLVTTVALTQD
                     NRLLVEEIGPYASQSAGKEYYKHIEKVIVDNDVYEKSLEGERTFDINYQGIKINANLI
                     KDGKHELTIVNKKDGDILITFIKKGDKVTFISAQKLGTTDHQGSLKKDVLSDKTVPQN
                     QGTQKVVKSGKNTTNLSLITKLSQEDGAILFPEIDRYSDNKQIKALTQQITKVTVNGT
                     VYKDLISDSVKDTNGWVSNMTGLHLGTKAFKDGENTIVISSKGFEDVIITVTKKDGQI
                     HFVSAKQKQHVTAEDRQSTKLDVTTLEKAIKEADAIIAKESNKDAVKDLAEKLQVIKD
                     SYKEIKDSKLLADTHRLLKDTIESYQAGEVSINNLTEGTYTLNFKANKENSEESSMLQ
                     GAFDKRAKLVVKADGTMEISMLNTALGQFLIDFSIESKGTYPAAVRKQVGQKDINGSY
                     IRSEFTMPIDDLDKLHKGAVLVSAMGGQESDLNHYDKYTKLDMTFSKTVTKGWSGYQV
                     ETDDKEKGVGTERLEKVLVKLGKDLDGDGKLSKTELEQIRGELRLDHYELTDISLLKH
                     AKNITELHLDGNQITEIPKELFSQMKQLRFLNLRSNHLTYLDKDTFKSNAQLRELYLS
                     SNFIHSLEGGLFQSLHHLEQLDLSKNRIGRLCDNPFEGLSRLTSLGFAENSLEEIPEK
                     ALEPLTSLNFIDLSQNNLALLPKTIEKLRALSTIVASRNHITRIDNISFKNLPKLSVL
                     DLSTNEISNLPNGIFKQNNQLTKLDFFNNLLTQVEESVFPDVETLNLDVKFNQIKSVS
                     PKVRALIGQHKLTPQKHIAKLEASLDGEKIKYHQAFSLLDLYYWEQKTNSAIDKELVS
                     IEEYQQLLQEKGSDTVSLLNDMQVDWSIVIQLQKKASNGQYVTVDEKLLSNDPKDDLT
                     GEFSLKDPGTYRIRKALITKKFATQKEHIYLTSNDILVAKGPHSHQKDLVENGLRALN
                     QKQLRDGIYYLNASMLKTDLASESMSNKAINHRVTLVVKKGVSYLEVEFRGIKVGKML
                     GYLGELSYFVDGYQRDLAGKPVGRTKKAEVVSYFTDVTGLPLADRYGKNYPKVLRMKL
                     IEQAKKDGLVPLQVFVPIMDAISKGSGLQTVFMRLDWASLTTEKAKVVKETNNPQENS
                     HLTSTDQLKGPQNRQQEKTPTSPPSAATGIANLTDLLAKKATGQSTQETSKTDDTDKA
                     EKLKQLVRDHQTSIEGKTAKDTKTKKSDKKHRSNQQSNGEESSSRYHLIAGLSSFMIV
                     ALGFIIGRKTLFK"
     gene            complement(37538..39067)
                     /locus_tag="VHD02_000519"
     CDS             complement(37538..39067)
                     /locus_tag="VHD02_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ5497151.1"
                     /translation="MNKNKLLRVAMLLSLLAPTAESMTVLAQDVMLETHKATTNETSD
                     SSSKEENNKNAAPTTSDKTDQGPLDASAETNSNSLVNADDKKRSDSSQSAVGSSDNKA
                     EAENQVDDKSTDHSKPTDHSKPTDQPKPTDQPKPSPSKVDTAPASSLSRQLPEVRTPI
                     QSLSPYVSDLDLNEIDIPSVNTYAAYVEHWSGKNAYTHHLLSRRYGIKADQIDSYLKS
                     TGIAYDSTRINGEKLLQWEKKSGLDVRAIVAIAMSESSLGTQGIATLLGANMFGYAAF
                     DLDPTQASKFNDDSAIVKMTQDTIIKNKNSNFALQDLKAAKFSRGQLNFASDGGVYFT
                     DTTGSGKRRAQIMEDLDKWIDDHGGTPAIPAELKVQSSASFASVPAGYKLSKSYDVLD
                     YQASSYAWGQCTWYVYNRAKELGYRFDPFMGNGGDWKYKVGYALSKTPKVGYAISFAP
                     GQAGADGTYGHVSIVEDVRKDGSILISESNCIGLGKISYRTFTAQQAEQLTYVIGKSK
                     N"
     gene            complement(39154..40254)
                     /locus_tag="VHD02_000520"
     CDS             complement(39154..40254)
                     /locus_tag="VHD02_000520"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEQ5497152.1"
                     /translation="MISSFHRPTVARVNLQAIKENVASVQKHIPLGVKTYAVVKADAY
                     GHGAVQVSKALLPQVDGYCVSNLDEALQLRQAGIDKEILILGVLLPNELELAVANAIT
                     VTIASLDWIALARLEKKECQGLKVHVKVDSGMGRIGLRSSKEVNLLIDSLKELGADVE
                     GIFTHFATADEADDTKFNQQLQFFKKLIAGLEDKPRLVHASNSATSIWHSDTIFNAVR
                     LGIVSYGLNPSGSDLSLPFPLQEALSLESSLVHVKMISAGDTVGYGVTYTAKKSEYVG
                     TVPIGYADGWTRNMQGFSVLVDGQFCEIIGRVSMDQLTIRLSKAYPLGTKVTLIGSNQ
                     QKNISTTDIANYRNTINYEVLCLLSDRIPRIY"
     gene            complement(40251..40607)
                     /locus_tag="VHD02_000521"
     CDS             complement(40251..40607)
                     /locus_tag="VHD02_000521"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEQ5497153.1"
                     /translation="MIVGHGIDLQEISAIEKVYQRNPRFAQKILTEQELAIFESFPYK
                     RRLSYLAGRWSGKEAFVKAIGTGIGRLTFQDIEILNDVRGCPILTKSPFKGNSFISIS
                     HSGNYVQASVILEDKK"
     gene            complement(40723..43242)
                     /gene="secA"
                     /locus_tag="VHD02_000522"
     CDS             complement(40723..43242)
                     /gene="secA"
                     /locus_tag="VHD02_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEQ5497154.1"
                     /translation="MANILRKVIENDKGELRKLEKIAKKVESYADQMASLSDRDLQGK
                     TLEFKERYQKGETLEQLLPEAFAVVREAAKRVLGLFPYRVQIMGGIVLHNGDVPEMRT
                     GEGKTLTATMPVYLNAIAGEGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PAEKREAYNCDITYSTNSEVGFDYLRDNMVVRQEDMVQRPLNFALVDEVDSVLIDEAR
                     TPLIVSGAVSSETNQLYIRADMFVKTLTSVDYVIDVPTKTIGLSDSGIDKAESYFNLS
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEDGEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKEGVRIQEESKTSASITYQNMFRMYKKLAGMTGTAKTEEEEFREVYNMRIIP
                     IPTNRPIARIDHTDLLYPTLESKFRAVVEDVKTRHAKGQPILVGTVAVETSDLISRKL
                     VEAGIPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGSDRIKAFLDRMKLDE
                     EDTVIKSGMLGRQVESAQKRVEGNNYDTRKQVLQYDDVMREQREIIYANRRDVITANR
                     DLGPEIKAMIKRTIDRAVDAHARSNRKDAIDAIVTFARTSLVPEESISAKELRGLKDD
                     QIKEKLYQRALAIYDQQLSKLRDQEAIIEFQKVLILMIVDNKWTEHIDALDQLRNAVG
                     LRGYAQNNPVVEYQAEGFKMFQDMIGAIEFDVTRTMMKAQIHEQERERASQRATTAAP
                     QNIQSQQSANTDDLPKVERNEACPCGSGKKFKNCHGRKSFS"
BASE COUNT        13338 a         9083 c         7415 g        13594 t
ORIGIN      
        1 agaaaaaaac gatatctata aaagaaaacg ttttctctaa ctgttttgac ataaaaaaaa
       61 cgtataactt cgggaaaatt atcctgagat tatacgtttg acatctttaa atatcctcta
      121 aggcatcttt cactttatca aaaaaacctt ttttctttgg atgtaacatt ttttcaccac
      181 tcgcttctgc aaatgcttgc aaggcttccc tttgagcgtc attgagtttg gttggggtca
      241 caatattaac ggtaacatgt tgatcccctt gacctccacc acggagtttt ggtgctcctt
      301 tgcctttgag gcggaaggtt ttacctgttt gtgtcccagc agggatagcc atctcaacat
      361 cgccatgaac cgttggaatt tcaacagtat ctcctaaggc tgcttgagta aaagaaatat
      421 ctaagctgta gtaaatagtg gaaccattgc gttcaaattg cttacttggt aagacattca
      481 aaataacaaa taaatcacca taaggtccgc cattaaagcc agcttctcct tgaccttgta
      541 aacggatttg ctgaccagtc tctacaccag ctggaatctt aacagacact ttatgagctt
      601 gcttttcatg actagtacca tgacaggttt gacagggttc tttaatttcc ttaccactac
      661 catgacaaat atcgcaggtg acttgtcgac gcatcatacc aagtggtgtt tgggtgtcaa
      721 ttgtcattac acctgaacca tggcacttac gacaagttac tggagcggta ccaggttttg
      781 cacctgagcc taaacaagtg ccgcaggttg cttcgcgatt atagctaact tctttttcaa
      841 caccaaatac agcctcttca aaagataaat taacacgata ttgcaaatcg tcaccttgac
      901 gaggggcatt tgggtttcgg cttccaccgc caccaaagaa gcttgagaag atatcttcaa
      961 aaccaccaaa gccacctccg tcaaaaccac caaagccgcc tgctccgcca ccaaagccgc
     1021 cttgagcacc tgcagcgccg tattgatcat aagcagcacg cttttgtgaa tcacttaagg
     1081 tttcgtaagc ttcttgaaca tccttatact tttgctccgc tccagcttct ttattgatat
     1141 ctggatgata ttttttagac atttttcgat aagctttttt gatatcgtcc tgagaagcgt
     1201 ccttagaaac ccccagacga tcgtaatatt ctgtattgtt cataatgtaa gataccaagc
     1261 cgtaaaacga ctcaatgaaa aataggaaat ccgacaaaga tactctcgta tctaggaggt
     1321 atttctcttt tttcgcaggg tcgtaggcgt gttcaaatcc gcaaaagagt ctttacacgc
     1381 tttagcctgt gttcgaataa tcgaacttgt attcctttct taaaatattt tgacgtcagc
     1441 atttttacat tttatttagc caaaaacaga ggtttggtac aacctctgaa ttcggatatt
     1501 aatgttacta gataactaaa tcattacttt tctgtaaatt cgccatctac aacatcatca
     1561 ttatttgctg aatcattagc ttgtgcacct tctgcacctt gtgctgcttg ttgagctgct
     1621 gcagcttgct cgtacatttt aacagccaaa gcttgcgctt tttcatttaa tgcttcaagt
     1681 ttagctttca tgtcgtcaag gttgccagat tcttgcgcag cttttaactc gtcaagagct
     1741 gattgcgctg catcgcgttc tgtatcaaag cctttacctt cagtttcttt gattgttttt
     1801 tcagtagcaa agatagcttg gtcaacttcg tttttaaggt caacttcttc tttacgtttc
     1861 gcatcggctt cggcattagc ttcagcgtct ttcatcatgc gatcaatttc ttcttcagaa
     1921 agtccgtcgt ttgatttgat aacgatgtgt tgttcctttt gcgtaccaag gtctttagct
     1981 tttacagaaa caataccgtt tttatcgata tcaaatgtta cttcaatttg tgggattcca
     2041 cgaggtgcag ctgggatatc agtcaattgg aagcgaccaa gagtcttgtt atctgctgcc
     2101 attgggcgtt caccttgaag aacatggata tcaacagctg gttggttgtc tgctgctgtt
     2161 gagaagactt gtgatttaga tgttgggata gtggtgttgc ggtcgatcaa tttggtgaag
     2221 acaccaccca ttgtttcaat accaagtgac aatggtgtta cgtcaagaag gacaacgtct
     2281 ttcacatccc cagtgataac accaccttgg atagcagcac ccatagcaac cacttcatca
     2341 gggtttacag atttatttgg ttctttacca gtttcagctt ttacagcttc gacaactgct
     2401 gggatacgag ttgatccacc aacaaggata acttcatcaa tttctgacaa tgacaatctt
     2461 gcatctgaaa gggcttgacg aactggagtt ttcgtacgtt ctacaaggtc gcgagtgaga
     2521 tcgtcaaatt tagcacgaga taagctcatc tccaagtgaa gaggaccggc agaaccagca
     2581 gtgatgaacg gtaatgaaat ttgtgtttgt gtcacacctg aaagatcttt tttagctttt
     2641 tcagcagcat ctttcaagcg ttgaagtgcc atcttatctt gtgataagtc aatgccattt
     2701 tctttcttaa attcagccac taagaaatca ataatttttt ggtcaaagtc gtcaccacca
     2761 agtttgttat cacctgctgt tgcaagaacg tcgaagacac catcacctaa ttcaaggatt
     2821 gatacgtcaa atgtaccacc accaaggtca aaaactaaga ttttttcatc cttgtcagtc
     2881 ttgtccatac cataagcaag tgcggctgct gttggttcgt taacgatacg ttctacttca
     2941 agacctgcaa ttttaccagc gtctttagtt gcctgacgtt gtgcatcgtt gaagtaagct
     3001 ggaacagtaa taactgcttt ttctactttt tctccaagat agtcttcagc ataaccttta
     3061 aggtattgaa gaatcattgc tgaaatttct tgaggagtat attctttacc atttgcagaa
     3121 actttttcag aagttcccat ctttgatttg atagagataa cagtatctgg atttgttacc
     3181 gcttgacgtt ttgcagcatc acccacgata atttcaccat ttttgaatga tactactgaa
     3241 ggagttgtac gattgccttc tgggttagca atgatttttg attcagtccc ttcaagaact
     3301 gctactgctg agtttgttgt acctaagtca ataccaataa ttttagacat gttaatacct
     3361 cttattttat aatactttat tactttttat ttcaatttat cttagtaagc actcgaatta
     3421 ttttacttcc aattctaagc aatcactata gtttaatcag caagttgaat agcttctaac
     3481 ttgctatcta gtttactgtc attacggaca aaaacgaaaa ctcatttacc ttagttataa
     3541 acaactacca tcgctggtct tagcagacgc tcgtgcaatt tatatccctt ttgaaagact
     3601 tctgcaatgc tatcggctgg atgttcatca tcggctggga gggtctgaac tgccatatga
     3661 aagttgtggt caaatgagtc gacttctact tcttctacgc cttcttcttt taatgcttga
     3721 ataaggctat cacgagtcat ttctaaacct tttttaacat catctgtcaa cccttcaaca
     3781 gccaaggcgc gttctagatt gtcaagcgat ggtaagattg cttttgccag atcctgagaa
     3841 cgatagcgtt gaagttgttg acgttcttca ctagagcgac gctgtatatt ctgcatttct
     3901 gcatgagctc gcaagtattt attttcaaac tcatcagcac gttcgtttac aagttctaac
     3961 tctgattttt caggagtttc ttcaataact tcctcaactg tctcttcagt ttcagtggct
     4021 tccacttctt cttgtaaatc gtctttttta atttcttctg acactgagtg cctcctttta
     4081 aataacttat tgaatactgc cattatcagt gaacttcata atggttagaa tttaggtatc
     4141 gataataatc acctaattta gcagccaata cctttccaat aatgtttacc aaactaacag
     4201 acctacgata atccatatca ataggaccaa ttaaactcag gagtccaaac ccacggtaag
     4261 gaatcaaaaa tttgtgagtt aaaactgaca catctgcaag tgcggcttct tgactatctg
     4321 ccacttggac agaagccatc tcaccttctt tcagactttg cctcaaagaa attgctactt
     4381 gctgttcatt atccaaaaac tgatacgttg atagatctga atacgttaaa gaatttacct
     4441 tgccagcaac aaataccgtt tccaaaaaca gctctgaaaa aacataatca aatagttgta
     4501 aaacattatc cgtagtcaca aaatactttt gaacgatctg aggaatttct gtcctcaact
     4561 tataatgaat atctatgaca ctattatcca gcaagcgctc ctctacgata gctttaaatg
     4621 ctatcaagtc acgtgtcaaa aagtttcttg gaatagcaaa ctgcactgtc actggcttag
     4681 attcatctaa agtcatgact gctagagcat catggttgct aagctgaaca acatcaaacc
     4741 ctgttaaacg ttgacgagct ggctccacat ctaaaataac ggatgtatag cccgtcatct
     4801 ccgctaaaat gtgactagct ttttgaagca tgtcttctaa tttgaatgct tcaaaatcaa
     4861 aagcttttat gacatgataa atatcttgct catcaatact atctagcctg agggagtgct
     4921 ctacaaaata cttaaaacca gctggacttg gcatccgtcc actagaagtg tgcgcttttt
     4981 ccaacaatcc caacttctct agttttgcca tatcattacg aatagtggcg ctactagaat
     5041 caatcgttct ctgtaaagct ttagacccta ctggttcatg agtttgtgta aatagctcaa
     5101 cgattaaatt caaaatatca ttttgacgtt gtgtaatcac aaaccaacca ctccctttcg
     5161 tttagcactc tatttagtag actgctaatt tataattcta ttatactctt tatgggagac
     5221 atgtcaagaa aaaaactcaa aaaaattagc actcttttat aaagagtgct aatttcagtc
     5281 atagtttgtt aaatcgttac tttcaatgac ctgaattagt cgcctagcat aggttttatc
     5341 agtataatgc aaatactttt gtaaactttt agctggtgct ttataccctt tttctgtagc
     5401 aagattacga tacaaacgct tatcgacttc tttgccctgc cgaagcattc gtaaataatc
     5461 tctaattgca ctagcatcat ctttataaat agcatatctc acgttttggt tggtttgttt
     5521 aggggacttt agggtaatgg ctgcttgtcc tggagttgct ttcttgctaa aaagattatg
     5581 atacttagac gctagtaatg tttttccatc atgggtttcc aaaagaattt gagcaatcaa
     5641 aatagatggt ctaatgccat aatattttgc caatggttta acttctttac ccatttttgt
     5701 aataaattgt ttttggctgt agttcaccct tacttcttta tctgcactag ctatcctttg
     5761 gctactcagc aaaggacaaa caatcagcaa taaaaagcaa gctagaagcg ttaaaaaata
     5821 cggaaacttt agtcgttttc tcatcgtttc tcctttaaag cagcaatgta ctcttccaac
     5881 gttaggttgt gctgagccat ataacgagca gacgccttac ctacatagcg gtaatgccaa
     5941 tcctcataat caacccctgt actagtcttt tttccttctg gaaagcgcaa gacaaaccca
     6001 taatcaggtg caattttttg aactgcttta gctactgatg gatcactggc atttaaagta
     6061 tctacagtgc tcatgtcaat agcaagacca gtgtgatgtt cactagtccc tggcggctgt
     6121 gaataggttt gcaccaaagc ctccgcagct tcttgtgtca atgttggatc gttagccatc
     6181 tcttttttaa tataagattg gtaaagctct gtttggtagt ctacggaacg gtacccagag
     6241 ataagatgtt cttgcgagtc gatagcttgt gctgctgcta aaaagtcact agtggcttgc
     6301 tcaatgcgtt tatccacaga aataccatta atgtcagcta attcaggact catttctttt
     6361 gtaatgtgat cgcggtttac taaaaccaac tcccaatctt caacagatac cttaggtaaa
     6421 gctcgtaaac ggtccgtttt tttgatatct ttttgttgga tttctttttt attcaactga
     6481 gttggtgtta ctgactcttc tggttttata aagaggtaaa gccctcctaa aaaaacaata
     6541 acaattagca attgcactaa atagtttaac gaattatatt tcatctgacc gttcttctat
     6601 tatttcacga ccgcggtagc ggtaactcat atctccaata gtctttatcg taaattggcc
     6661 atcatcaaat tcaacaactg taacactccc attatcaata tactgtttgt ctcgatctgg
     6721 atcaatcaac cacagaaacg ttccaattgt cataccatgg ctaaccacaa gagcatttcc
     6781 gccgccagca ttttgaatgg acaaggcaat agcttcaaac ccctcccaaa tgcgcttacg
     6841 aagaacttcc catggttctg cccaattagc ggtatctatt tccacgatgc tttctgccag
     6901 ttcagaatag ggaacatcac gtaggttatc acgattttca aaggcttttg ttcttggaag
     6961 gacacctaga aataattctg aatcataagt cccttctaga ctcccaaaac accactcacg
     7021 aatacgatta tctttagtat aaggcaaaaa ttcgttttct gattctctta aaataatttc
     7081 tattgtctgc atggttcgac ctgaatcact tgaaaacgca gccttaaatg gtatagttgc
     7141 atcttttaaa cctaagccaa gttctcgaat accttcttct cctttttttg taagaggtgt
     7201 gtcactccaa ccctgagcgc gaccaatggt attaaacatc gtttttccat gtctggcaat
     7261 atacaacctt gtttttgtca tttcaacctc ctaaactgtc ttattaatta ttatagcatt
     7321 taaagaaaac acttgcaaaa cacttcctga tggctatttg agatcaaata gaccactgac
     7381 ttattgtcta ttttattaaa aacaatgcta ttgctacaat tcaaatcatc acataaattt
     7441 ccaaacgatt acatttttgt gtttcgctca aatgtatata acaacagacc cacactacaa
     7501 ctaaactgac gttacaaaat gatgtcaaaa cagtaacaaa atctaactat ttgtcaagaa
     7561 aacgatatta aattttcaca caactatctt ataatgagga tgcaaccaat aaaaagataa
     7621 gacattttat gttgcaaaaa aggctgcttg gaaacttcca tgcagccttt ttttaatacc
     7681 tttttccaaa aatagctgaa acaagtacaa ttacaattgc tgctcccaaa atggacggga
     7741 aaatagccat acctgccaac tgaattcccc aatccccaaa aagcctttgc ccaacagctg
     7801 accctatgag acctgcgata atattagtaa aacaacccat ggactggctt ttttcagtca
     7861 ttttacctgc tatcatgccg ataaaggcac ctacaattaa agaccatatc atcgttaaca
     7921 cctcctttct tgtaaaatcc ttttagaaga actgctatcc attaaatgtt cttaacttta
     7981 gttgattcct ttaccttctt ctttagtaag tgtttccgtg cctgagtaaa atagaaaaaa
     8041 caattccttc tattaagata gatagaccga gaacgactag aagtcttcac acactaaata
     8101 acttaacaaa ctttatcaca cagctaatcg ctaataatag tgtagacaaa cgtgatggtt
     8161 cattaaatac ccgtcagaaa ctcctgaagg ttctaaagca atacctgtca gtcttatttt
     8221 agcatagatg atggtctttt tatagacggt cactaacatg acatacaaaa aggcagcctg
     8281 tgaaagaccg accgcctttt tttcagagac aaaatacttt aacttattgc tatcagcaac
     8341 tatcaatctc gcaatttttg caattcttgt gctaataatt gttgagcgac ttgtggatta
     8401 gcttgacctt tggttgcctt catcaagaag cctgtgaaag ccttatcagc attacgtttg
     8461 cctgatttga aatcagccac tgcagcttca ttatcagcaa agacttggtg aataattggt
     8521 accaaaacag cgggatcaga aatctgaaca agtcctgcct tttcgacgta ggcgcgagct
     8581 gagccaccat ttttagccaa atgcacaaag accttcttag ccattttaga agagattgta
     8641 ccatcagcaa tgatagcaat catctccacc aaattttctg gtgtcagagc gatttcttca
     8701 atggttttgc cttctgcatt taagaattgg gcaacttctc cttgcaacca gttagagact
     8761 tgcttggcat cgccaccaag acttactgca gtttcaaagt aatctgacaa gactttggta
     8821 gctgttagct ggctagcatc ataagctgag agaccaagtt cctcttcgta tttagcacga
     8881 cgttgagctg ggaattgagg taattgagca cgcatctcgt caatccaagc atcatcaatc
     8941 tcatacaagg gtaggtctgg ttctgggaaa taacgatagt ccgctgcccc ttctttgacc
     9001 cgcatcaaaa tagtcccctt attcgcttcg tcatagcggc gcgtttcttg gcgtatcacc
     9061 ccacctgaac gcaagagttt ggcttgacgt tccacctcaa attcaagccc tttacggaca
     9121 tttgagaagg agttcaagtt tttcaattca gtctttgttc caaattgttc ttgcccgtaa
     9181 ggacgaaggg aaatattggc atccacccgc atagagcctt cttccatctt gacgtcagaa
     9241 atacccgtat actggatgat ttctttcaga gctgtcaaat aagcataggc ttcttcagga
     9301 gaacgcatgt cggcttctga cacaatctca atcaaaggaa ctccttggcg gttaaggtct
     9361 acataagaat aaccatctgt gccgtgagtg tttttaccag catcttcttc caagtgtgcg
     9421 cgttcaatac gaattttctt ggttgaaccg tcttccaatt taatcttaat ccatccgttg
     9481 tagccaatcg gctcatcaaa ctgtgaaatt tgataagctt taggattatc tgggtagaaa
     9541 taatttttgc ggtcaaagtg catctcttta tggatgtcca tattgagggc aagcgcagct
     9601 tttatgcccg catcaataac gcctttgttc atgactggta agacccctgg aaaagaccaa
     9661 tcaatcacat tcgtgttggc atttgggtcc tcaccaaagt gagctgacga aggtgagaaa
     9721 attttggaat tggtatttaa ttccacatgg acttcaagcc caataatggt ttcaaaattc
     9781 attacttgtc acctccaaaa ataatcggtt gttgcttgtg ataatctgtc accgcttcaa
     9841 aggctgctgc cgcttgatag atggtttctt cagcgtattt cggaccaatt aattgaagcc
     9901 ctactggcaa gccatcgacg aatcctgctg gaatggaaat tcctggcaga ccagccaagt
     9961 taactggaat ggtcaataag tcggccaaat acatggcaac aggatcgtgg ttgagtgtgt
    10021 ctaagccaaa agcaaccgcc ggtgttgttg ggcctaaaat aagatcataa tccgcaaaga
    10081 ccttgtcaaa atcttcaata atcaaggtac gcacttgacc ggcctttttg aaataagcgt
    10141 cataataacc tgatgacaaa ctaaaggtcc caagcatgat acgacgttta acctcatcac
    10201 caaagccttg gctacgagtg ttaacataga tttcatccaa gtttttagca tcgtcagcac
    10261 ggaaaccata gcgaatgcca tcaaaacgtt gtaagtttga ggacgcttct gacgaagcaa
    10321 taatgtaata gacagccaca ccatatttgc tatgtggaag actaacttcc tcaacagtag
    10381 ctcctaaggc ttcaaattgt ttagctgccg caagaaccgt ttccttaatc ttaggatcaa
    10441 tcccttcgcc aagatactct ttaggaaagg caatcttcat gcctttaatg tcacgaccaa
    10501 tcttagatgt gtagtctgca atgcggacag gcgctgaagt agcatccttg acatcgctac
    10561 ttgcaatcac atttaataat tgggcgtttt ctttaactgt cggagcaaaa ggtccaatct
    10621 gatcaagtga acttccaaaa gcaatgagac catagcgaga aacagtgcca taggttggtt
    10681 tcaaaccgac aacaccatta aatgcagctg gctgccgaat agaaccacca gtgtctgaac
    10741 caagagataa acggacttgt cctgaagcga ctgctgttgc cgaaccacct gatgagccac
    10801 caggtacttt tgtgtggtcc caagcatttt tcgttttctt gaaataagag gtttctgttg
    10861 aaccacccat ggcaaattcg tccatgttgg tttttccaat gacaatcatg tctttagcgt
    10921 aggcatttgc tactgatgtg gcatcaaaaa tcggctcgta gttatacaac atttttgaag
    10981 cggctgtggt taggatgccc ttggttgaga tattatcctt aacagctagt ggaatacccg
    11041 acatgaggtt atcagcatca atacctttgg catcgattgc cgcagcttgt ttaagagcag
    11101 cttcttctga aacagtgata aacgacccga cagcctcctc acgagacttg atatcttcaa
    11161 gtgttttttg agtcaattcg gttgcggaaa tctccttagc tacaaggagg tcatgcaact
    11221 cttcaatggt tttatgatta aaagacatta ggcatctcct ccgtcatcca aaatagcagg
    11281 taccttgata aagtgatttt ctttttcagg aacattttta aagagcaagg cacggtctgt
    11341 tccttcttca gcgacatctt gacgcatcac atttttctta tctgccatgg ttgttgtaat
    11401 ggcaacacct tcggtatcaa cttcgttcag caattcaacc atgtcaacga ttttagatag
    11461 ggtcgttgca aaggttgttg tctcactttc tgaaaatgat aattttgata gtttcgcgac
    11521 atggcgaact tcttcttcag aaattttcat gatttctccc atccaagatg acaagaagta
    11581 ttccaattcc aaagaacagc tctgctattc tccaaaggta gcggaaaaac tcctcacatc
    11641 atttttacat agtctcaatc attatatcat aattgcgccc atttgccata ggtgactaat
    11701 gcaattctag aaagccatat agcaaaaaac ttcctgacta aggtcagaaa accttagtca
    11761 ggaagtgtga caaactaaac tctatttatc ttgagtaaga ctgtggcctg aagctttgat
    11821 ggcagcctga gcagccgcta agcgagtaag cggcactcta aatggcgaac aagaaacgta
    11881 ggtcaggcct tggctgtggc aaaaggcaat ggaagcagga tcgccaccat gttccccaca
    11941 aataccaagt ttcaactcag gcttaaccga acgggctaaa gagacggcca atccctttct
    12001 gatcaaaggt ctggaacggt tccttatcaa aaaatacccc tgtcaacgta ttcttccaag
    12061 tattttccta caacatcaca gaaaaagtaa cgttctttca agtcggtaat atgtttacga
    12121 aggtaataaa ggggtgcttt attctaattt accaaatcct caacgattgt gacaacatgt
    12181 gatgtgataa gcctaagata aaaatggtac agactctagc attcaagagt ttgaagcttt
    12241 atttatattc taatgctgaa aagactagag tttgtctaat ggctgtgaga cgatcctcta
    12301 aaccatcaac tcaaatcatt tctcaaacag ctaaaatcct gtctccttca aaaaaacatg
    12361 tgttctatct gacacagtcc aaagtttagt tgccttttag acagcacaag aatcatcagg
    12421 actaattctg accagcatgt cacatacctc atctacctaa cccatacata cagtaaaacc
    12481 acatagagca atgattacgg ttaccaaact cttagtcact gaatcaatag tttggtaggc
    12541 tcttttagta atcgttaata agcttagcac caagaacctg ctcaaagtga gccaaagacc
    12601 actcataaga ttcttttgta aggctagcga cagccgattt aacaatctct aattgataac
    12661 caagatgata agcgtcaata gcagtatgta agacacagat atcagataaa actcctgtta
    12721 acactaattg agtgatacct ctttctcgta gtctaatatc taaatcagtc cccgaaaaag
    12781 ctgaataata acgcttatca atccaaaaaa ctctgggatg ttgcttcata taactgtaaa
    12841 cttcagctag tggaccataa agatggcgtc cagtagttcc tttgatatta tgtgctgcaa
    12901 ataacttact ctcaggatgc caaggatcat tttgatcatg acaatcaata gcaaaaaaaa
    12961 tatagtctcc ttgatcaaat gccgttttag ttacctctgc tattttagtg gcaatagcct
    13021 gagcagattt acctgcactt aatttaccat catctgctac aaaatcattt gtgtaatcaa
    13081 ttgaaatcaa tgctctcatc actctctcct aaacagggaa acctctcccc ttatctctta
    13141 tatatggcat tgtatcagtt tttattacag gtatcaagac atctatttgc atctaccatt
    13201 aacagcaaaa aacatctctt ttcaagagat gttgtctttt tctatttaaa attcttttaa
    13261 tttagcaaaa ataccttcgt tgataacttt gaggtatgtc cctttcatac caagagaacg
    13321 actttcaata atccctgcac tttctaattt acgcagagca ttgacaataa cagaacgggt
    13381 aataccaata cgatcagcaa taacagaagc tgtcaatcgt ccttcgttac catctaactc
    13441 tccaagaatt gctgcaacaa ctttcatttc tgaataagaa agcgtattaa ttgccatgtt
    13501 gaccgctgtt tgtttacgga tggtgtcttc taagttttct gtctgaagat ttaatagttg
    13561 aatccccaca acagtacttg agatctcaac caaaatcaaa tcatcatcac taaactcatt
    13621 gtcattacgc cagataatga gtgatccaag gcgcatccct ccaccataaa tcggcgcaat
    13681 agtcgttaga cctcctggat aagtgtcttt agactcaaca gggaatatag tcaattcatt
    13741 ttcgacagaa aggttagctt ctgtatcgta aactcggcta gcagccttta cataagtatc
    13801 tggaaattgt ttagcttcaa aaaattcctc aacacgatca gtgttagttt tatatttcat
    13861 ggcataacca agcagtgtgc cgccgccatt gataatacaa gcattgcagt caatgatatc
    13921 tgctaggcga gatgccattg tgttatacgg taattctgtt tctaggctat ctacggaacg
    13981 ctgcaaaata gatgtgattt tacgagtttt ttctaataag ttaggcattt ttttcctttt
    14041 cctttgaatt atttgcgctt tcattataac aaagctaact cgcttttgca aggaattatc
    14101 agaaaatttt ctattcttac tacaattcca actattgatg ccttattatc acttcattta
    14161 aaggagataa gcactagctt gttagagcat agggtcacat tcttttaaga aacgttattt
    14221 ttattaatca aagatgagca ttgcattaac gtctatactg gtataagaca cgtgtaatct
    14281 tttcttggac atcttctaat tcttctacac ttggtaggta aacaatacgg aagtgatctg
    14341 gatctttcca gttaaaacca cgtccatgta ccaacatcac tttttcttgc tttaataact
    14401 gtaagacaaa ttcttcatca tcatcaatac gatacatctg acgatcaatt ttcggaaata
    14461 aataaagacc tgcctcaggt ttaactgccg ataagccagg gatagcatta atagcttcat
    14521 aaataaaatt acgttgctca aaaatacgcc ctcctggcag taacagtgca tcaactgatt
    14581 gatgacctcc taaggaagtt tgaacaactt gttgggctaa aacattggag cataaccgca
    14641 tattggcaag catgtttaac ccttcaatgt aacctttaac atggtcttta ggacctgata
    14701 agaccatcca tccaacacga aaaccagcaa tacgatgaga tttggaaagg ccattcatag
    14761 atacacaaaa aacatcaggt gccaaacttg caatagcaat atgctcttta ctatccatca
    14821 ccaaacgatc atagatttca tcggcaaaaa taatcagctg gtgttctctt gccaaagcaa
    14881 cgatgtcttc taaaatctcc ttaggataaa gagctcctgt tgggttatta ggattgatga
    14941 ccacaatagc tttggtgcga ctcgtgattt ttgatttgat atcagcaata tcaggatacc
    15001 aaccagcttc ttcatcacaa agataatgga ccgcttttcc tccaccaaga ctcacacaag
    15061 ccgtccataa aggataatcc ggcattggta ctaaaacttc atcgccatta tctagcaaag
    15121 cttgaaggga catggatatc aactctgaca cgccatttcc caaataaata tcttcaatgt
    15181 caacatctgg gaagcccttg agttgacaat attgcatgat agccttacgc gctgaaaaaa
    15241 tccctttact gtcagaatag ccctcactta gcctcgcgtt gacaattaaa tcacgaatca
    15301 cttcatctgg tgcttcaaaa ccaaaggcag caggattgcc agtatttaaa cgcagaattt
    15361 tttcaccact cgcaatcatg cgattagctt catccaaaac aggccctcga atatcatagg
    15421 caacatgttc taattttgat gatttttcaa taattttcat agtctcatac acctcgtatt
    15481 ccatattata aaccaaaata cttaaaatag aaagaattta tccatcaaag cttaggacag
    15541 ctaatcgttt taacacaggt cttatgagac gataatacag tctttagttt agcaaaaata
    15601 atggatcgct ttcaaaatga ctcaaaaaag agtataatga aaaaaagaat aaaagctatt
    15661 cttgtcaaac tgttaaagga ggacagttct atgtcactaa aatataaacg tatcctcgtt
    15721 gctattgatg gctcttatga gtctgaatta gctttcaata aaggcgttaa cgtagcacta
    15781 cgtaacgacg ctaccttact actagtacac gttattgaca cccgtgcctt gcaaagcgta
    15841 gctacttttg atacttacat ctatgaaaaa ttagaacaag aagctaagga cgttttagat
    15901 gattttgaaa aacaagcaca gatagcagga attacaaaca ccaaacaaat tattgaattt
    15961 ggcaatccca aaaacttact agctcacgac attccagatc gtgagaacgc tgatttaatt
    16021 atggtaggag ctactggtct taataccttt gaacgcctcc ttattggctc atcttctgag
    16081 tacattatgc gacacgctaa gattgactta cttgtagtcc gagatagcac taaaacactc
    16141 tagtctatta caattttcaa acaccaaaaa acaagcagca tctacaacga aattgcttgt
    16201 tttttggtgc tgagctaagt ctagtcaata tctttttatt tcttgatatt taaaagagta
    16261 aacctctatt tatcgaatcg ttgtagtgat ttttgtaatt gtttgtctaa ctcttttttt
    16321 atggctactt caggtgcgtg acgctcttgc caatagtcag gtttttgaat tttatgggta
    16381 acggggtcaa agtgtgcctt gccatctgga aaaattttag ccatatttgc ttcatgtact
    16441 gcttcaaata taggttgtgg atctactccc atcagtacaa aagaaccata agtaaaataa
    16501 agcaaatccg ctaaggcatc aacttgccca atcaggggcg tttcaacatg ttttttagct
    16561 tgtaccttat taactgcttg gtcaacagca ccatgcaaat cgaaaatagc ctgtgtaaat
    16621 tgttgttggt ctcccttgct tgctgcgtaa agaaactcaa caatttcttc tactttgaag
    16681 cctgacctat aaccagcttc tgaaatggta taactacgcg gtgtttcaat agtatctgag
    16741 tccatcagac gatgaaaatc tttgacttta ttaaaatttt catcacggct tttaaagctc
    16801 ttctctacat caaaatggat gagaccgtag tgagctaagg ctttggaaat cccatcatta
    16861 ttattagagt cggtagtgaa atgggctcca tcttttacca cagtttcagc atttcccata
    16921 gcaataccga taccaactcc tgataacatt tcaaggtcat tatctgaatc tccaaaagcc
    16981 atcacttcag acaagtcaaa tccaaacatc tctcccaaac gttcaattcc ttttattttt
    17041 gattgatcaa cagagatcag atccaaggaa taaggactac ttcttgtaat ttttatatga
    17101 gggaacttct cttgtatttt cttagtttca gcttgtgacg ctaccagaac aacctgataa
    17161 ataggctccc gcataatagt tactaaattt ctaaaactct gaggcttgat tcggcgaatc
    17221 aaatgcttaa aactaccctc aactgttctt gcccaacttt tgggcacaaa actactaatg
    17281 acttgtccaa atgggctagt ccccatatca ataatacgcg atcctgctaa tccagaagct
    17341 gttccaagag aaatttctcg ctttttttcg ttggcatagc gaatcacttt gtaaatcatc
    17401 gactttggca attgattctg atataagacc ttatctcttg ttaagatata ttgcccattg
    17461 taagtaacgg caaaatctaa tccaaaattt tctagaaaag gttgcacaaa tcccggtcca
    17521 cgaccagttg ctaaaccgac cataatgcct tgttttttga gttgttggat ggctttttgc
    17581 gttgtttttt ggatattttt acgatcattg agaagggtcc catcaatatc aaaaaagact
    17641 gcttttactg tcaaaacctt cttttgcctc ctgtgtttat gatagaatag attatatcat
    17701 aaataaaagg aagtttgaca atcaaatgaa aaaaatccta gtcttacaca ctggtggaac
    17761 catttctatg caagctgata actctggtag ggttgttcca aatcaagata atcctatgac
    17821 gaaaatacat gctgcagccc aagatattca gcttaccgtt agtgattttc taaatcttcc
    17881 gagtcctcac atcacgcctc accacatgtt atctatttat catcatattc aagaaagaac
    17941 tgatgtcttt gatggtatag tcatcacaca tggaacagat accttagaag aaacagctta
    18001 ttttttagat accatggcat tgcctactaa tatacctgtt gtcctaacag gtgctatgag
    18061 gtcttctaat gaagttggta gtgatggcat atataattac cttaccgcac ttagagttgc
    18121 aagtagtgat aaagctaaag aaaaaggggt acttgttgtt atgaacgatg aaatccatgc
    18181 tgctaaatat gttactaaaa cacatacaac caatatttca actttccaaa cacccacaca
    18241 cggaccactt ggtatcatca tgaaaaatga cctacttttt ttcaaaactg ctgaacctcg
    18301 tattcgtttt gacttgagat gcatttctgg aaccatcccc attatcaaag catacgcagg
    18361 tatgggcgat ggtagtatct tgagtctatt aactccaggt agcattcaag gtctagttat
    18421 cgaagctcta ggagcaggaa atgttccacc attagcagtt ggggagatcg agcacttaat
    18481 tactttaggt attcctgtcg ttcttgtttc ccgttgtttc aatggtatgg cagaacctgt
    18541 ctatgcttat gaaggtggtg gtgcgatgct acaagaagca ggtgttatgt ttgtaaaaga
    18601 attgaatgct ccaaaagccc gtctaaagtt attaattgct ctcaacgccg gacttactgg
    18661 tcaagagcta aaagattata ttgaaggata atctagcttc ctgtagaaat gtcccactaa
    18721 aacatattag tgggacattt ctagtacttt aagctttaaa ttaggacatc tcctaaatgt
    18781 ctataataaa caactatgtt tcaaactatg agattgtgca ctcataatca tttctataac
    18841 agtttgagac cagtattcca ataaatccaa ctcttagcaa tcagataccg ggtaaacaag
    18901 tctttatttt gtctcattaa gtataagctt acttgaataa taccaaaact acttgctcat
    18961 ccttttttag taatgatgaa gacgaccttt ttctaagaac ttctttgtct tttaaaataa
    19021 acaaagcaca tattaccctt tattttagtt ctcgtttgga aaaaagaaag acattgccta
    19081 aaagataaca aagagttaaa ccaaaggcca ctaatatgct agtactagaa agatgtttat
    19141 caccaagaga caaggatagg tgtttaaaca tcaaaactgg gctaatatct aacttaaaaa
    19201 aatggtttgc taaagtagca gatactttat caaaaagaag gacataaagt atataagctg
    19261 tgattgactg ataagtttta cgaagtatat taaacaaaaa ttgacacaaa gcaataatag
    19321 caatataaaa aggcagttgt ttaatcatcc gatctatgac tattccatag tatgcttgac
    19381 cagtaaataa atgaaatgct aaagcactaa gaagataagt taaactaata aataaaagag
    19441 ataagccaaa actaactagc agtttataga ttataattaa agtacgattt tgttgtttga
    19501 ccacaacatt attaatcgta cggtagttat agtcatcact aaaaaagata cgggctggtg
    19561 ttaaaaagaa aagcgtcaca aaatcagtaa taatattaat gatttgtatc atgatgactt
    19621 ggttaggtgt cttgcgaaaa ctataggcta caccaacagc aatgacaaca agtagcatac
    19681 aagaaaatag agctaaacgc tcttggcgta tccgaaataa atctgcttta agataggaaa
    19741 tcataaaaac ctctttctaa attaatggtg acgctgcatc tttacctaac aaatcgaggt
    19801 aataatgttc aaagctcact tgatgatgat aaatgtcaaa aatctcaaca ggcagatcta
    19861 gaacaatttt gataagctcc attactgata aagttggttt tattagtagc atctgaccat
    19921 cagtctcaac tctaagcccc aattctaaaa gtctattttt taccagctga tcatcgcttg
    19981 tatgaagggc aatttgtact tttacttgag ctttaagctc tgctttgtcc atactcttaa
    20041 taactttgcc tttatgcata atcacgtaac gatcaacaac taagtctaat tctgataaaa
    20101 tatgactgga aatcaaaatc gtaataccat agcaatctct taggttcaaa ataatctgac
    20161 gcatttcttt gattcctgct ggatcaagac cattaatagg ttcatctaag attaaaaatt
    20221 ctggaaaatc cagtatcgaa agaccaatag ccaaacgttg gcgcatcccc aaagaataat
    20281 ctttcacctt tttctttttg ctcacttttt ctagcccaat aagttccaaa acctcatata
    20341 tgcgctcttt tgcatcagct aatctcaatt gcaacgctgc atagtaaaga ttatcatgag
    20401 ctgtcatatt gggataaagt gtcggtgact caataattga tcccattcga tagggttgta
    20461 actgtttgat ttgccctgaa tcttttccaa ttaggccaga taaaatacgc attaacgttg
    20521 tctttccagc accattttga cccacaaggc cgcaaatttc tcctttgtaa atatcaaaag
    20581 agatatcact taaaacctga tttttaccat aggatttatt tacttgctca atacttagta
    20641 acattgattg ctgtgtcatt ttccgctcct tttttgtact tatcgattaa tacaatatct
    20701 tacacataat aacatctgta aacggttgtg acaagttctt ctttttatta aaaaaatata
    20761 aataagaatt ttaataatat gattatttta gcaataaaaa agttatactg aaaatgggtt
    20821 aatactgctt cacatagcag tctcatcgta aagcataagt aaaaaaggcc agaaatagtg
    20881 acctctttat actttataac agtttatctc aaaactaact aaatatttgt aaaacaacga
    20941 cttgttttaa gttaatcata aacttctttt tttctaatat tttgtgccac taattgccac
    21001 tgtttttcat atatccagtt aggatcagaa acaatcgctg cagaaacttt gcgtgcttct
    21061 tctaaaatag gataatcctc aacgatatca gctacttgaa attctggaat tccagactga
    21121 cgagtaccaa agatttcacc agaaccacgc atttttaaat ccgactcagc taaaacgaaa
    21181 ccatctgtcg tttctgtcat gattgtcatt cgttttttcc ccgaatcagt tttgggatta
    21241 gccactaaaa cagcgtatga ttgtttatat ccacgaccaa cacgcccacg aagttgatgt
    21301 aactgactta atccaaaacg atcggcatcc ataataatca tgatcgttgc atttgggaca
    21361 tttaccccta cttcaataac tgttgtggat actaaaatat gacttttttt atccttgaaa
    21421 tcttgcattc tggcatcttt ttcatcattt ttcatacgtc catgtacaag agcaacctta
    21481 gcaattcctt caaaataagt agataattcg gcatgcaatg ctactgcatt ctttaaatct
    21541 aaagcttctg attcttcaat caacggtgaa atgacataca cttgagcatc tttttgcaat
    21601 tcacctttta cccattccaa cacagtccct agctgctcgt gtttcaccca gcgtgtcata
    21661 ataggtttac gaccggcagg taattcatca ataacagaaa catccatttc tccaaaagct
    21721 gtgattgcta gagttcgggg aattggggtg gctgtcatca ttaaaacatc aggattttct
    21781 cccttttcac ggaaaattct acgctgttta acaccaaaac gatgttgctc gtctgtaatg
    21841 acaagcccca gtttatggta ctgtaccgag tcttggataa gagcatgagt tcctacaatc
    21901 atatccactg agccatttgc aatagctgct aaaaccgtac gcttgacagc ggctttcata
    21961 cccgaagtta atatagcaat tgatagatct ggaaataact cttgcagact aatgtagtgt
    22021 tgttcagcta ggatttccgt tggaaccatc aaagccgatt gaaaacctgc tgtataagct
    22081 gcatacattg atagaccagc aatgactgtc tttcctgatc ctacatctcc ttgcaataaa
    22141 cgattcatat gagctcccga tgacatatca gataatatgt catctaaaga gcgcttttga
    22201 gcatttgtta gaataaaggg taaagaggaa atctttgtct ccatagcatg tttactataa
    22261 agaataggca aaccatttgt ttcagattta ttttcggctt tcaaaacttg aaggttcatt
    22321 tgaaagtaaa ataattcttc aaatttaatc cgacggagcg cttgcttata ctctgtgata
    22381 tcttttggga aatgcatagc taaacaagcc tgactacgac ccatcaatcg gtatttttcc
    22441 aataaagtag ctggtaaatt ttccttcaat tccaaatgcg catcgatttc aaaagctgac
    22501 ttaatagctt taatcaaagt agactgtgaa attccctgag ctacgcgata aacaggttgc
    22561 atgtcatctt caacttgagc taaaaccttc atcccagtaa tagccgattt agtggcatcc
    22621 catttaccaa aaacagctac ctcttgacca agttctattt tatcagctaa atagggttga
    22681 ttaaaaaaac taacatttaa gacagcttcc ccttgacgca atttgaaact taaacggtta
    22741 cgtttaaaac cataatattg tacattagct ggagtaacga ctaagcctgt aatgactgct
    22801 ttttcaccat ccactaaatc aaaaacagat tttgatttaa aatcttcata gcgaaacgga
    22861 taataaagca gtaaatcttc tactgtataa atatctaatt tctgaaattt ttctgctgat
    22921 tttggtccaa atccctttaa gttggacata ggagctgtta atatcattat ctttttccct
    22981 tcacaaaaac tcttgtcaga ttgaagacaa agtttgtcgt gatactttcc ttaggtggtg
    23041 cttacggcag cgacgttctt tggaattccc tgcctttatt tttgagcctg aggtctcaaa
    23101 aatcccgagt ggcgtcaatc tcgtattgac gccactcttt taccacagca aaaagcatct
    23161 gttttggctc actttatatg taaaaatcgg aaaccgaatg tcaaatcagc agagctatgc
    23221 atgctttttg aacataaaac tggggttgtc tacgttctga ctcttgtatc tattatacaa
    23281 gagttcctat ttattttcag ataaaagcct tttagtactt ttaaacagtt ctaggggctc
    23341 gttaaggtag catttgataa atggtatcgc agacactact tagaagttct tcatcatgtg
    23401 aaatgaggat gacaacctta tgttgggtct tcagctgcat gagaagatta gctaaagcct
    23461 tcatttgtaa caaatcaagt ccgctacttg gctcatcaaa aattaagata tctttatcag
    23521 ccaaaagact agcggcaatc atcactcttt gttgctcacc tcctgacaaa ctagctggat
    23581 gacgttctaa aagagtcgtt aaagataacc gctctattac ttctgtcgtc cgtggatgtc
    23641 gttcatggcc caagttgact tcctttgaaa ccgattctgc aaatagttgc aaagaaactt
    23701 cttgcaagac aatactagtc tttgataaac gttgcttggc agataaaggt atgccttgaa
    23761 acttaatagt tgctttttta tcgtctaaga taccagctat ataataaact aattgcgatt
    23821 ttccaagccc gtttgatcct gtaatgccag aaatacttcc tgcagcaaaa ctaagctgtt
    23881 caatacatct aagtattttg cttcccgctc taactgttag atgctcaata cacaggctat
    23941 catctgggcg ataatgttga ctctcaatct taccagctaa cacaggtttt aggtcagaca
    24001 agtctaagcg acgtagtccc atatcttgcc gttgttcatc tgtcaaggct agcaaatctt
    24061 gtgttgttaa cttatctgtt aatctaccat ttttaaagta gaaaaagtta tctgccaaat
    24121 ccatcaagta atgtaaacga tgctcggcta caataatggt ttttcctgct gcttttaatt
    24181 gggtaagata ggctttaaca gcagctatgc ctgcactatc taagttagca gttggctcat
    24241 caaaaagcat aatattagta ccttgcataa tggccgttgc aatagcaact ctttgttttt
    24301 gccctcctga caatccaaac atatccttgt taaggagctc tgcaaaagca aaatcttctg
    24361 ctaaagtcca aagacgcttc atgataacct ttgcatctag accttgattt tcacagggaa
    24421 agaccagttc gtgttggact tcgcgataga aaaactgact ggctggattt tgaaaaacag
    24481 aggccacaga tctagaaaat gtttcaacac tgtctctgcc tgcttggcaa tcagccacat
    24541 ctagtctacc ttcatatttt ccagcataat aatcaggaat tatgccatta agaagtttta
    24601 aaaaggtaga tttacctgaa ccagaaggtc cacacaaaac aatgaattgt ccagatgcaa
    24661 tttgacaagt cggtacctga caagctggat ttttttgatc atgataggta aaaacaagtt
    24721 gttctgctga aatcattgaa gtatcgtgat gattatgata acagtaatcc aaatctgcac
    24781 cccccaatct ttccatgtta agtgtgagct aaagcattca ctggtgcctt tgttgactgc
    24841 tagcccttta gttagactag cgatagttag ttcttggcta cttcttatca aagataaaag
    24901 caatggaacc attaaatact ccaagtattg tttaggtctg atcaaaatag accattttgt
    24961 taaaataatc cccctgattg ttaaagagcg atgaataaca caacattctt gtctaatcat
    25021 tggtatgaaa cggcacataa cggctaaggt caatagccag acttctggga aatgccattt
    25081 gcgtaatcca tgaaccaatt cgtagatggt agttgtctta atagtaatta aacctgctag
    25141 taaagaaggg aaaactaacc ttcctgctat tgagacaaaa ctcaataagt gatcaagata
    25201 agaagggaaa atagatagcg ggattacaga tagaccgatc attaacagat aaacaaccgc
    25261 caacttgaac gctagctttt tcttatttaa agcagacaaa agtaataaga gaaaaataac
    25321 aattataaat tctaaaaagc catcaacacg aaaaaagaag caggcattgg caagtaccaa
    25381 caaaagcagt ttggtgcgaa catcaagcct catgataaca atcctgattt gataaaatgt
    25441 ttctttagta aataatgtgc aaaagctgac cctaataagg ctcctgcaag aacaattcct
    25501 acaaaccaaa taacatgccc taaactaaaa tcatacataa tgcgattgac ataggtcata
    25561 cttttaccgc gggctaacaa actttcaaca taggcatctt tcaacaacca catcagcaaa
    25621 atagggccac tattgacaaa agcaaaaacc atataagata ggtcattaaa aaaccttgac
    25681 ttgtattttc ctaaagaagc aataatatcc gctattaaac cgaaaataat gctgggaaac
    25741 atcgcggcaa aaaagtgacc actaaggaag aaaaaacctc ccatcatcag tcctaataat
    25801 gttattggcc caaatgtttg caatttagca atcaaaagaa aataaattgt tccccccagt
    25861 aaagctgcaa aggcaggggc atataacata tttcctgagc gatcaaaaaa gatgttaata
    25921 aacgtgccta gaccaacaca aataaagtag agcgcggcaa atgcgcctgt tatcataata
    25981 tcttttattg ttaactgttt cattttttat tcacctcttt taggaaacca ttttttacgc
    26041 aaaatagatg atcagcacaa gctagggttg atggcctgtg agaaattaag aagaccaatc
    26101 cttgacaatg tgttttaatt aaatcaagca agatagcttc atttagagaa tctaaatttg
    26161 acgtgggttc atcaaaaata tagcaactcg catctttcaa taaagctcgc atcaactcta
    26221 aacgttgacc ttctccagct gaaaagtcag aaggactctt gataatcgta tccaaacctt
    26281 ccttacaagc taacagtctg tctttcatac cacaagcctc agctaaattc caaatggact
    26341 catctgaaat atcttgacgg ccaaaggtaa gattttcacg gatagactgt tggaaaagct
    26401 gcgctgtttg tggcacataa gcaatagtat cttgtaattt agcagcattt agtaagcaac
    26461 tattcctatc attaagaaaa atatctcctg tcttccaatt ataccatttc ataattaatt
    26521 tcaccaaagt tgatttacca gatcctgatt cgcctttaat gccaataatc cccttttctt
    26581 gaaaggtaac tgataaatcc ttaaaaacca gctcttggcg ctttggataa gcaaaactga
    26641 ggtcctcaaa agaaacactc cttaaacgat ctatttgttt tgtgccttca tctactatga
    26701 cattttcgtc tagaaggtca aaaatatttc gagccgcatt catagcacgc ttaaaaccca
    26761 atggcaaacg tcctagttct aaaaaaggag aaaacgaagc agtgaaagca ataaaagtta
    26821 ataagccttt atcaaatgat aagctgtcat ggcatatccc atcaaaaacc atataagaaa
    26881 atgctaagat cattaagcca agccagaaaa aagtcaatgc catttgcaaa aattgggctt
    26941 gcgctacgtt acgatcaaga gcatttacat gattactttt ttttattaat cgttcaaatt
    27001 gctcgtctaa tacctgaaat tgcaaaagat ctttgactga tcgtaaactc tctaaaaaat
    27061 aagacaggta atcttttcga ccttcatttt ggctttttag taaaacctgt aaaatattag
    27121 caaaataaat gggtataaca atggctaaac aagcataggt tgccaaagct aataaggcta
    27181 actgccaact actttgacaa aagtaccaga acattaaacc tgcagacaat attgctgtac
    27241 aaataggcgc gattgtgtgg gcaaaaaaaa cttctaatgc ctcaatatct tctccaatca
    27301 ttttaagcaa ataaccactg tcttgactat ccaagcaagc tggagaaaga gctcttagtt
    27361 tcttaaagat caagttccta aaagctgcta aactatgaaa agcaacaaaa tgaccaaaat
    27421 aatgctctcc ataacgaaca aaacctctta aaaaggctag agcaatcaaa agatataaag
    27481 ctaatattgg aatcacttct tgtctaatag ctagtagtcc taaataagct attccagttg
    27541 gaatacttac cgtgatgaca aaacccataa cagcaaagcc aacagccaag ataatccaag
    27601 gtagcagagt tttcattagg gttaataaac gccaaaccag gcttaaggta gatctgttag
    27661 gtttagccat aaatacttgc ctccagtctt gcttgagtat caactaggtg acgataagtt
    27721 ggttggtccc gataaaggtc gcaaggtttg cctaaacaag caggctgccc tgtgcttaaa
    27781 aagagtactt ggttagctcc tttcagtcga gacatcttat gagtaatcac aataactatt
    27841 gccgtcttag ctaatctcgt tattaaattg tcaataatac gttcattttc tgcatctaga
    27901 ctagatgttg cctcatcaaa gatataaaga gacctcttac ttaatagagc tcttacgcaa
    27961 atcacctgtt gcttttggcc tggtgataac agcttaccat tttcaccaac tattgtgtca
    28021 aggccatctg gtagccagtt gatgaagctt aacaaaccat gttgatctat ccaatctaag
    28081 atttcttttt tagagagatt tgcggctaac cttaaattat cgtaaatgct acggttcaat
    28141 aaggtagatt ggtccgatac ataaagaacc tgctggttga taatttcttg cgacaaattg
    28201 tcactgtcta aaccgtcaaa taaaatatgc ccagtcgttg cagactggcg tttcaagagg
    28261 agttgagcta gacttgtttt tccttgtcct gacacccccg cgatagctgt tagttggccc
    28321 ttcgttaatg tcatggtaac cccactaagc actgtttttt tctcataagc taaagaaata
    28381 ttttggatgt caatctggtt aaagtttatt gctgttttac tcttgttatc tttccttgct
    28441 ggaacactat ccaagaaact aaagatacgg tcagccattt tggtattcat catgaccaag
    28501 tgcatgccat aaccctgctc acgaatcggt gtaaaaaatt ctgtagcaat caagagaaaa
    28561 aagaggaaat taaaaaaaga taactgacct gttgccaaag cttgaacggc taaaaaacct
    28621 gacaaaccaa ttcccagata cataactgca tccatataac caactgcttg taactgaaag
    28681 ccaagcaaag acatggtagc ctttcgaaac tgttctgcct tacccgaaaa ttcttgctcg
    28741 taacgttctg ttgcttgata agaatacagt gtattaagac cttttaagtc atccataaat
    28801 aaattaccaa catccatata agaagaccaa taatgactca taatttgctt agaccgcttt
    28861 tgcatagcaa cgattgaaag tggaatcaga ggcagaactc ctaaaaagac aaggcctgcc
    28921 aatggataaa ttaaaaagac tagtatcata atagttgtgc agttccactt ggttctcata
    28981 gacaaagaca aatagtaacc ataataagta tccaaactat caatcccctg tgaagccaac
    29041 gttaaaatat cagcatcact ggcatgagca tcaaattgcc cgtctaaatc aataaaagcc
    29101 tcaaagaaag attgtttgag tgaatctcta gcaaattgtg aagcgatccc ttgcagttgc
    29161 ttgcctaata tagcaagaac aaagcctacc acatttaaaa gtaggagtaa acctgccaag
    29221 cgtcctaagg caatagcatg acccaaaata aaggtcgaaa acgttttggc aatgaggtaa
    29281 aaactgatca tcctcatcac aaattgtaac caagataaaa aggctgacac ataaagcaaa
    29341 tacctttttg gtgcaattcg ttcacgtaat cgttttaaca gtcttttcct ttttcccctt
    29401 gatattgtat cttctgtttc catcatctgt tcttgttcct atctctcctt tgagttctaa
    29461 tatcattggt ttcattgacc aatgaagtga ggacaaactg ttgaccattt ctctcaacaa
    29521 agggcaattc aacaccaaag acatctctga taaactgttg atcaaggagg tctcttggtt
    29581 gcccttgggc ataaagtcgc ccatctttca gtccaataag atggtctgaa agagacaagg
    29641 cctgattgat gtcatgtaat actaacacaa tcgttaagcc tagctcttgg ttgattttag
    29701 taatcagctt gagaatgtca agctggtact taatatccaa ataagtcgtt ggctcatcta
    29761 aaaaaataat atctgtatcc tgggcaaggg ccatagcaat ccaaaccctc tgctgttcac
    29821 caccagaaag gtgttggatg tcacgataaa cataatcact caaagccgtt acttctaagg
    29881 cccaagcaag gcgttcctca tcttctttgg tagtagtttt aaaaaaagaa cgacgggcaa
    29941 gcctagccat ggctaccaat tcacccacta acaaaccttc gaccacttga tggtattggt
    30001 gtaccactgc cacttttttc gcaaaagcct tgagagaaat cgttgctaat tcttcctgct
    30061 caagccaaac ctgcccttgt tttaacggaa ggtttttggt taataattgt aataaggttg
    30121 acttgccact gccatttgcc cccataatag tggtaatttt cccttttgga atgctaaagg
    30181 tcaggtcatc taaaacaagt ttttcctcat aagcaaagga aaggttttgc acctgaatat
    30241 gactgctgtt cttactcata taatatcaga cctccttagc aggtaaataa agtaaggccc
    30301 gcctacaata ctcattataa tcgcagggct aatttccaat gggtaagcaa gacttcttcc
    30361 taaagtatcg gctagcaaaa agacaaaggc tccaagcaaa gctgaaaaag gaattaagat
    30421 ttggtgctta cttccaacca gcaagcggct catatgaggg acaatcaagc ctaggaaaga
    30481 aaccactcca acgatcgaag tggctaccga agccaataaa acagcaacca aggaaattcc
    30541 caaccgtaac gctgtagcat cgattcctaa atgacgaatc acctgatcat ctaaaagaag
    30601 aaggttacag gtttttgaca ataacaaagc tagtaacaag ccaatgaatg tataagggaa
    30661 taaaacccct acatcagccc atgttttttg actaatatga cctgctaaaa gagcattgac
    30721 catgggacta gctgaggtga aaaaactggt taaagcagtt gataacccca taaataaagc
    30781 attaatagca atccctgtta gaataagccg aataggatta agccctttct tccatgctaa
    30841 accataaatc agcaggaaag acactccgcc tcctaaaaag gatacgattg gaccatacag
    30901 cagcaactgc ggtagaagta aactagagac taaaaccata aaactagcac cactacaaat
    30961 ccctgtaata gcagggtctg aaataggatt tttcaacact gcttgaaaaa gaactcccga
    31021 aacagctatc cctgctcctg ctaataaagc aatgacaatc cttggaaaac gcaaatcata
    31081 aatcaaagcc acttgggggt cataagtcac aaacagtccc ttgacaattg ccccataaga
    31141 aaccgccaaa ccaccaaggg ataaggcaat aaccgccaaa aataggaggc tgagaattaa
    31201 aagaactaga taaagaccac ttctgaaacg ttttttagcc agtagggatt gatccctttg
    31261 taaatctgat gctgtcatgt tacggatgat ctcccacgtg gtcaaaaagc tgtgttaagg
    31321 tgtccaaggc ttctgggtag ttcaatttag cactcatgcc aaacagggta ttgtccaaat
    31381 catagacttt cccttccttg actgccgtaa aatgtttcca aatatcattt tcagcaaatt
    31441 ctttgtcaaa catcactttt accttgtctg gaatggcgtg agctgttcgt aaaatcaagt
    31501 caggctcctt agccagcatg tcttcaggat taactgatag aaattctttc tcatctgact
    31561 gataaacatt ctcaccacct gccaaatcca aaagattccc tacataagat tggttcgtcg
    31621 ccaccaaata actacctggc aagcccataa gaataagcac tttaggcttt ttcttcccct
    31681 tacgtttagc ttggaaagca cgataatagt cctggtattg ctggcgcaat tcttttgctt
    31741 cttgttgacg ttggaaaagg ttccctaaat catcaatgga ctggtacatg ccctcaacac
    31801 ttcgtaagtt caaaaaacca tactcagtat ccaatttttg atacttgggt tccaaatctt
    31861 cttgtaaaga attgggactc aaaatccaag ttggtttcaa agaagcaatc aactctatat
    31921 caggattcat gggtaaaccc acacgcttaa cagcatcata gcgtttagga agggtatata
    31981 atttactatc acaaacccca acgaggtcta aatttaaacg gtcacagata tcaaccacag
    32041 caaccgaagt ggctacaatt ctctgctgtt cagtctcttt agccgtttta gggtgctgat
    32101 tcacacaagc taccaaaaag aaggccatta aggctagacc aattccttta catcgtttta
    32161 tcattttttt tccctctttt tccaaaccca tacagcaaac cctcctaaca tcagaaaaaa
    32221 agttggaaag tagtaaatca tgattgataa gtctttttta gaccgaaact tatcttcttt
    32281 ttttaactct aaagaagtcg ttaagcccat cttagaagga agaatttttt tatctgatag
    32341 actcttgtta tttgatgagt tggctttagg cttattctga gttatcatag cacctgtgtg
    32401 agactcatct tgactttctc ctagttttgt atcaacaggc ttttgactgt cttttacttc
    32461 ttgattttga gaattgtctg tttcagtaac aagctgggcc aacatattac ctgaatactt
    32521 ttgtattaac tctgaagctg acaggtaaaa gaccacttct ctgcccatag gttcaacaaa
    32581 catgcttcct cgaatgatac tatttacagt aggcaaacta atagcaatat ctagtgttgt
    32641 cccattggta tcagtacctt tttgggtgat attgtaatca actgcctgaa agcttccagt
    32701 tcctccaggt tgtatccaga actgataatt gcccgaataa tcagctaagc tcatacgaaa
    32761 agtaagaacg atctttccag catcagatac ttctagcatg gcatcactat agacagtccc
    32821 ttcaaccata ccttgaccaa tatcaaagga atgctcccct ccgctatctt ctatctgccc
    32881 agatatcgga tgacgataat ttctctgaat aatacatcca taaatttgac ctttatctgc
    32941 atacacaaca cctgtcattg tcgataaact tatcatgaac actactatta cctgcaacaa
    33001 ttgctttatt actacctttg tcatatgctc ctcttgtcta tcttttctta aacatcttat
    33061 ttcatttttt aaatgaatcc cattttataa catctccaaa tgtgaaaaac acacctatct
    33121 tgcgactact tcaacctgta tcaggagtcc tgtcggaaat aggtatgttt ttagctcttg
    33181 ctgactagct tttttgttat ttcacttggt actaagtttc ttatttaaat aatgtctttc
    33241 gaccaataat gaatcccaga gctacgatca taaagctaga tagacctgca attaagtgat
    33301 aacgagagct actttcttca ccatttgatt gttgattgga acgatgtttc ttatcagatt
    33361 ttttagtctt agtatctttt gccgttttac cttcaattga tgtttgatga tcacgcacta
    33421 actgcttcaa tttctctgcc ttatcagtat catctgtctt agaagtttct tgagttgatt
    33481 ggccggttgc ttttttagcc aggagatcag ttaagttagc aataccagta gctgctgaag
    33541 gaggacttgt aggtgttttt tcttgttgac gattttgagg tcctttcaac tgatctgttg
    33601 aagttagatg gctattttct tgtggattat tagtttcttt gacaaccttt gccttctctg
    33661 ttgtaaggct tgcccagtct aaacgcataa aaacggtttg aaggccagac ccttttgaaa
    33721 tggcatccat gataggcaca aagacctgta atggcacaag tccgtctttc ttcgcttgtt
    33781 caatcaattt catacgcagc acttttggat agttttttcc ataacgatct gccaatggta
    33841 ggccagttac atccgtgaaa taagacacaa cctctgcctt ttttgttcga ccaactggtt
    33901 taccagctaa atctctttgg taaccatcta cgaaatagct caattcacca aggtagccta
    33961 acattttacc aacctttata cctctaaact caacttctaa ataggaaaca ccttttttaa
    34021 ctaccaaagt cactcgatga ttaatagcct tgtttgacat ggactcagat gctaagtcag
    34081 tttttaacat gctggcattt aaatagtaaa taccatcacg caattgtttt tgatttaatg
    34141 ctctaaggcc gttctcaact aaatctttct gatgtgaatg tggtcctttc gccacaagga
    34201 tatcattaga tgtcaaatag atatgttctt tttgagtagc aaatttcttt gttattaaag
    34261 ctttgcgaat ccgatatgta cctggatctt ttaaagaaaa ctctcccgtt aagtcatctt
    34321 tcggatcatt tgagagaagc ttttcgtcaa ccgtcacata ctgtccattg gaagcttttt
    34381 tttgcaactg aatcacaata ctccaatcga cttgcatatc attaagtaaa gaaacagtat
    34441 ctgaaccttt ttcttgtaac aattgttgat attcttcaat agacactagt tctttatcaa
    34501 tggcagaatt tgttttttgc tcccaataat acaaatctaa aagactgaaa gcttgatgat
    34561 attttatttt ttcgccatct aaagaagctt caagttttgc aatatgtttt tgtggagtca
    34621 gtttgtgttg tccgataaga gctcttactt ttggactcac actttttatc tgattgaact
    34681 tcacatctaa attaagcgtt tcaacatctg gaaatactga ttcttcaacc tgagtaagca
    34741 agttattgaa aaaatcaagt tttgttaatt ggttattctg tttaaatata ccatttggaa
    34801 gatttgaaat ttcattagtt gataaatcga gtacagataa tttaggaaga tttttaaatg
    34861 aaatattatc aatacgagta atatgatttc tacttgccac aatagtgctt aaggcgcgca
    34921 atttttctat tgtttttggc agtagtgcta aattattttg agataagtcg ataaaattaa
    34981 gtgatgttag aggctctagc gctttttcag gtatctcctc aagactattt tctgcgaaac
    35041 ctaatgaagt cagacgagac aatccttcaa atgggttatc acaaagtcgg ccaatacgat
    35101 tcttggaaag atcaagttgc tccaggtgat gaagcgactg gaatagtcct ccttcaagag
    35161 agtgaataaa gttacttgat aagtagagtt ctcttaattg agcattgctt ttaaatgtat
    35221 ctttgtctag ataagttaaa tgattacttc ttaagttaag aaatcgaagt tgcttcattt
    35281 gactaaataa ctcttttgga atttccgtaa tttggtttcc atccagatgt agttctgtaa
    35341 tatttttagc atgtttcaat aaagaaatat ctgttaactc gtaatggtct agacgcaact
    35401 cacctcgaat ctgttctaat tccgtttttg ataatttacc atcgccgtct aaatctttgc
    35461 caagtttaac taaaactttt tcaagacgtt cagtcccaac ccctttttct ttatcatcag
    35521 tttctacctg ataaccactc caacctttgg taacggtctt actaaaggtc atgtcaagtt
    35581 tggtgtattt gtcatagtgg tttaaatcac tttcttgacc tcccatggct gataccaaaa
    35641 cagcaccttt gtgtaattta tccaaatcat caataggcat ggtaaattcg cttcgaatat
    35701 agctaccatt gatatctttt tggccaactt gtttacgcac tgctgctggg taggtccctt
    35761 tgctttcaat agaaaagtca atcaaaaatt gtcccaaagc agtattaagc atggaaattt
    35821 ccattgtacc atctgcttta accactaatt tggctctttt atcaaaagca ccttgaagca
    35881 tggaggactc ttctgagttt tctttattag ctttaaagtt tagcgtatag gttccttctg
    35941 tgagattgtt aatagaaacc tcacctgctt gataagactc gatggtatct tttaacagtc
    36001 gatgagtatc ggcgagtagc ttactatctt tgatttcttt gtaagaatcc ttgatgactt
    36061 gaagtttctc agccagatct ttgaccgcgt ctttgttgct ttctttagca ataatcgcat
    36121 ccgcttcttt gatagctttt tccaaagtgg tgacatccaa ctttgttgat tgtctgtctt
    36181 cagcagtcac atgttgtttt tgtttggcag atacaaaatg gatttgacca tctttcttgg
    36241 taacggtaat gataacgtct tcaaatcctt ttgaggatat cacgattgta ttttctccat
    36301 ctttgaaagc ttttgttcca agatgaagcc ctgtcatatt cgagacccag ccattagtat
    36361 cttttacaga atctgaaata agatctttat aaactgtacc attgactgta acctttgtga
    36421 tttgctgagt caatgctttt atctgtttgt tatcagaata acgatcaatt tctggaaata
    36481 aaattgcacc atcttcttga ctcaattttg ttattaatga caagttagta gtatttttcc
    36541 cagatttaac aactttttgt gtgccttggt tttgtggcac tgttttatca ctgagcacat
    36601 ctttttttaa tgaaccctga tgatctgttg ttcctaattt ttgagctgaa ataaaggtca
    36661 ctttatcgcc ctttttaata aaggtaatta ggatatcacc atctttttta ttaacaatag
    36721 tcaattcatg cttaccgtct ttaataaggt tagcattgat cttaatccct tggtagttaa
    36781 tatcaaaggt tcgctcgcct tccaggcttt tttcatagac atcattatca acaataacct
    36841 tttcaatatg tttataatac tcttttccag ctgattgact agcgtaaggg cctatctctt
    36901 caactaaaag tcgattatcc tgagttaaag ctactgttgt cacgagttgt gactgaacta
    36961 aagcctcttg tgattttaca gtttgagtag cctgaatgag aacaagggca gatattgcca
    37021 caaacgcaca tttgctgatt tttttcattt tcaatcaact ttctaaaaat ttttatgaat
    37081 aagctaactt ttttattaac tgaacttaat ctatcacttt gtgattattt tgtcaagatt
    37141 gtttccaatg gtccaattgt ctgttaaata gctccactat gtgagctaag acactagtaa
    37201 taagcctgga ttgataatga ggatatctct gataaaagaa ttcctctaca acaatattac
    37261 tgagataaag ctagccttag gatagttatt actagggctg attcaatcat tacttcattc
    37321 acaaaacatg taacacgtta taagctccat aattgtgtta ggagacaaca tgggatgtca
    37381 ctactgcata ttcatcacaa taaaaaaaga caatagccct aatcgtgcta ttgtctcaaa
    37441 atcatttatt tacttgaaac tttatcgtgt tacaccaaca gtttaaaagg cctaactccc
    37501 gctaagaaaa ttaagccgta agaaaagtca ttagttatta gtttttactc ttaccaataa
    37561 catatgttag ctgttcagcc tgctgagctg taaaggtacg ataagaaatc ttacctaagc
    37621 cgatacagtt agactctgaa ataagaatag acccatcttt tctaacatct tctacaattg
    37681 atacgtggcc ataagtgcca tcagcgcccg cttgccctgg tgcaaatgaa atagcataac
    37741 ctacttttgg agtctttgaa agggcatacc ctactttata cttccaatct ccaccatttc
    37801 ccataaaagg atcaaatcgg taacccaatt ctttggcgcg attatacaca taccaagtgc
    37861 attgtcccca agcataactc gaagcttgat aatccaagac atcataactc ttagagagct
    37921 tataacctgc tggcacagat gcaaaactag ctgatgactg cactttcaat tcggctggaa
    37981 tggctggtgt gccaccatgg tcatcaatcc acttatccag gtcttccata atttgtgcgc
    38041 gacgtttacc actaccagta gtatcagtaa aataaacacc cccgtcactt gcaaagttta
    38101 attgacctcg tgaaaactta gccgctttta aatcttgaag tgcaaaattg ctatttttgt
    38161 ttttaataat ggtatcttgt gtcattttga caatagcact atcatcatta aacttacttg
    38221 cttgagtcgg atctagatca aaagctgcat agccaaacat attagctcca agcaaagtcg
    38281 caatcccttg agttcctaaa gaactctcag acatcgcaat agctacgata gctcgaacat
    38341 ccagcccact tttcttttcc cattgcaata gcttctcacc attaatacgt gtgctgtcat
    38401 aggcaatgcc tgttgatttt aagtaactat caatctggtc agctttaata ccataacggc
    38461 gagataaaag atggtgggta taggcatttt taccactcca atgctctaca tatgccgcgt
    38521 atgtgttgac agaagggata tctatctcat tcaaatctaa atctgatacg taaggtgaca
    38581 acgactgaat aggagttctt acctctggca actgtctcga caatgaagaa gcaggtgccg
    38641 tatcaacttt agatggtgat ggtttgggct gatcagtcgg ttttggctgg tcagttggtt
    38701 tcgaatgatc agttggtttc gaatggtcag ttgatttatc atctacctgg ttttctgctt
    38761 ctgccttgtt gtccgaagaa cctacagcag actgactaga atcgcttctt tttttatcat
    38821 ccgcgttaac aagactatta gagtttgttt ctgcagaagc atcaagggga ccttggtcag
    38881 ttttatctga tgttgtaggt gctgcatttt tattattttc ctcttttgaa gaagaatcac
    38941 tggtttcatt tgtagttgct ttatgcgtct caagcattac atcttgagcc aacactgtca
    39001 tgctttctgc tgtcggggct aagagactta gtagcatggc aactcttaat agtttgtttt
    39061 tattcatata ttaattagtc acttctttct aatgtcctca ctttattata acattaaaga
    39121 taagcacaat gcaattattt ttacgtgatt ttcttaatat atccgaggaa tacggtcact
    39181 taaaaggcat agaacttcat aattgattgt attacggtaa tttgcgatat ctgttgtaga
    39241 aatatttttt tgctgattgc tgccaatcaa agtgactttt gttcctaaag gatatgcttt
    39301 ggaaagtcgt atggtcagtt gatccatcga tacacgccct ataatttcac agaattgtcc
    39361 atcaactaac accgaaaagc cttgcatgtt cctggtccag ccatctgcat aaccgattgg
    39421 gacggtccct acatattcag actttttggc agtataagta actccataac cgactgtatc
    39481 accagctgaa atcatcttga catgcactaa gctagattct agagataaag cctcttgcag
    39541 tggaaatggt aagcttagat cagaaccact tggattcaaa ccataactga caattcctaa
    39601 acgaacagca ttaaaaatgg tatcactatg ccagatactt gtggctgaat tactagcatg
    39661 tactaaacga ggcttatcct caagtccagc tatcagcttt ttaaaaaact gtaactgctg
    39721 gttaaattta gtatcatctg cctcatcagc tgtagcaaaa tgagtaaaaa taccttctac
    39781 atcagcaccc aactctttta gactatcaat caataaattg acttcttttg aagaacgaag
    39841 cccgatccgc cccataccag aatcaacttt tacatgaact tttaagcctt gacattcttt
    39901 tttctccagt ctagctaaag ctatccagtc taaactagcg attgtaacag taatagcatt
    39961 agcaactgct aactctaatt catttggcag caaaacccca agaattaaaa tctctttatc
    40021 aatacctgct tgacgtaatt gcaaagcctc atcaagattt gacacacagt acccatccac
    40081 ttgaggtagg agtgcttttg acacctggac agcaccatga ccataagcat cagccttgac
    40141 aactgcatac gtttttaccc ctagtggaat atgcttttga acactggcaa cattctcctt
    40201 aatagcttgt aagttgaccc ttgcaactgt tgggcgatgg aaacttgaaa tcatttttta
    40261 tcctccaaaa taacactagc ctgtacataa ttgccactat gtgagatgct aataaaacta
    40321 tttcctttaa acggagattt tgtcagaatg ggacatcctc taacatcatt taaaatctca
    40381 atatcttgaa aggttaaccg cccaatacct gtccctatag ccttaacaaa agcttcctta
    40441 cctgaccagc gtcctgccaa atagctgagt cgacgtttgt aaggaaaact ctcaaaaata
    40501 gccaattcct gctcagttaa aattttttga gcaaagcgag gatttctttg ataaactttt
    40561 tcaatagctg aaatctcttg taagtctatt ccatgtccaa caatcattac tttccaccaa
    40621 accttatttc atcttaatgt tagaagattt aaacagtgag aagctgagaa ttattctcag
    40681 cttctttcta tatacctaac tttgtcctgt tgtgtctcgt tcttatgaga aggatttacg
    40741 accgtgacaa tttttgaatt ttttgcctga accacatgga caagcttcgt tacgctccac
    40801 tttgggtaaa tcatcggtgt tagctgactg ctgcgattgg atgttttgag gtgctgctgt
    40861 tgtggcacgt tgacttgcac gttcacgttc ttgttcgtga atttgtgctt tcatcatggt
    40921 acgtgttaca tcaaactcga tagcaccaat catatcttgg aacatcttga aaccttctgc
    40981 ttggtattcc accacagggt tattttgagc atacccccga agtccaacag cattacgaag
    41041 ttgatctaag gcatcaatgt gttcagtcca tttgttatcc acaatcatca aaatgagtac
    41101 tttttggaat tcaataatag cttcttgatc acgaagtttt gatagttgtt ggtcatagat
    41161 agcaagcgct ctttgataaa gcttctcttt gatttgatca tcttttaaac cacgtaactc
    41221 tttagcacta atagactctt ctggtaccag cgaggtccta gcaaaagtta caatagcatc
    41281 tatagcatct ttacgattgc tacgtgcatg agcatctaca gcacggtcga tagtacgctt
    41341 gatcatagct ttaatttctg ggccaagatc tcgattagca gttatgacat cacgacggtt
    41401 agcatagata atttcacgct gttcacgcat cacatcatcg tattgtaaga cctgtttacg
    41461 ggtatcgtag ttatttcctt caacccgttt ttgagcagat tccacttgac gccctaacat
    41521 acctgattta ataacagtgt cttcttcatc taatttcatc cgatccaaaa aggcttttat
    41581 acgatcagag ccaaaacgtc tcatcaaatc atcttctaat gaaagataga attgggattc
    41641 acctggatct ccttgacgac ctgaacgtcc acgaagctgg ttatcgatac gacggctttc
    41701 atgacgctct gtaccaatga cacataaccc accaagttca cgaacacctt caccaagctt
    41761 gatatcagta ccacgaccag ccatgttagt tgcgattgta acagcaccac gttgcccagc
    41821 attcataatg atttgagctt ctttaaagtg atttttagca ttcaaaacct catgaggaat
    41881 ccctgcttca accagcttgc gtgaaatcaa atcactagtt tcaacagcaa ccgtacctac
    41941 aaggataggt tgtccttttg catgacgcgt tttaacgtct tccacaactg ctctaaactt
    42001 tgattctaag gtcgggtaaa gcaaatcagt gtgatcaata cgggcaattg gacgattggt
    42061 tggaattgga ataatacgca tgttataaac ttcgcgaaat tcttcttcct cggtcttcgc
    42121 agtaccagtc atacctgcca attttttgta catacgaaac atattttggt aagtgattga
    42181 ggcgctggtt tttgattctt cctgaatacg aacgccttct tttgcttcaa tagcttggtg
    42241 caagccgtct gagaagcgac gcccttccat cgtacgacct gtaaattggt caacaataag
    42301 aatctcacca tcttcgctga ccacataatc gatatctaac aacataatat agtttgcacg
    42361 aagggcgttg tctataaagt gagtcaaggc tacattttca atatcgtaaa gattgctcaa
    42421 gttaaagtaa ctctcagctt tatcaatacc tgaatcgctt aatccaatcg ttttagtggg
    42481 cacatcaata acataatcca ctgacgtcag agtttttaca aacatgtcag cacgaatata
    42541 caattgattg gtttcagagc taacagcacc agaaacaatt aaaggtgtcc ttgcctcgtc
    42601 aattaagact gagtccactt catcaaccaa ggcaaaattg agtgggcgtt gtaccatgtc
    42661 ttcctggcga acaaccatgt tgtcacgaag gtaatcaaaa ccgacttcag agttggttga
    42721 ataagtaata tcacaattat aagcttcacg tttttcagca ggagacttag cagctaggtt
    42781 gatcccaact gaaagaccta accagctata cacttcaccc atttcggtag cgtcacgcgt
    42841 tgaaagatac tcattaacag taataacgtg aacaccctca ccagcaatag cattgagata
    42901 aactggcatg gtggctgtaa gagtttttcc ttcaccagta cgcatttcag ggacatcccc
    42961 gttatgaaga acaattcctc ccataatttg aacacgataa gggaacaagc ccaacacacg
    43021 ttttgctgct tcacgaacaa ctgcgaaagc ttcaggtaat agttgttcga gggtctcacc
    43081 tttttgatag cgttctttaa attcaagtgt ttttccttgt agatctctat ccgacaatga
    43141 cgccatttgg tctgcgtagg attctacttt ttttgcaatt ttttctaatt ttcttagttc
    43201 gcctttgtca ttttcgataa ctttgcgtag aatattggcc attctctttc ctttcgatca
    43261 tctaatcatt tcattttaac ataaatatgc aggcagttca agaaaggttg gtaaagttac
    43321 acgccgtacg catgaaaact ttctcttcat ccttttgtgc ccggacatca attttgaaaa
    43381 gattcctttt taaatcaatc atctgcttag cctctttctc caaataattg
//
LOCUS       DAUDYX010000010        41183 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.010, whole genome shotgun sequence.
ACCESSION   DAUDYX010000010 DAUDYX010000000
VERSION     DAUDYX010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41183
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.010"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(<1..328)
                     /locus_tag="VHD02_000523"
     CDS             complement(<1..328)
                     /locus_tag="VHD02_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEQ5497155.1"
                     /translation="MLVLYVSSKLIKRRRMKFMYSALLGNPVEHSISDELFEYLNHNI
                     GVLDEYKHKKILVDSVCLEDKIKHLINDPKCIGLNITLPYKREVMRYIDNLDRVAEIT
                     GSVNNIY"
     gene            complement(397..468)
                     /gene="shp3"
                     /locus_tag="VHD02_000524"
     CDS             complement(397..468)
                     /gene="shp3"
                     /locus_tag="VHD02_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP3"
                     /protein_id="HEQ5497156.1"
                     /translation="MKKISKFLPILILAMDIIIIVGG"
     gene            548..1399
                     /gene="rgg3"
                     /locus_tag="VHD02_000525"
     CDS             548..1399
                     /gene="rgg3"
                     /locus_tag="VHD02_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Regulator gene of glucosyltransferase 3; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg3"
                     /protein_id="HEQ5497157.1"
                     /translation="MKSKLGATLRKVRKGKQISLCFVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLILHDEDYTNSETFANLVQYIRKQYSSQNINNIACLLSDT
                     SDYTLNSFEKTMVKSILHTMDSSIIPSDDELLQLTDYLFKVEKWGYYEILLLGNCVRT
                     INYNSYFLLTKEMLNNYIYSSRNKTNKRIVTQLAINCFILSINKEEFSNCSYLIDEIK
                     TLLDNELNFYEQTVFLYATGYFEFKRQLETGVEKMKQAIQVLDILNEDKLKLHYADHF
                     DKLVNKD"
     gene            complement(1652..5191)
                     /gene="smc"
                     /locus_tag="VHD02_000526"
     CDS             complement(1652..5191)
                     /gene="smc"
                     /locus_tag="VHD02_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEQ5497158.1"
                     /translation="MFLKEIELEGFKSFADKTKIEFDKGVTAVVGPNGSGKSNITESL
                     RWALGESSAKNLRGGKMPDVIFAGTQNRNPLNYAKVAVVLDNSDHFIKTAKKEIRVER
                     HIYRNGDSDYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQIKLNQTQDNLDRLEDIIYELDTQLAPLEKQAKVAKQFLEL
                     DANRKQLQLDILVKDIDIAQERQTKDTEALAALQQDLASYYAKRQSMEEDYQKFKQKK
                     QVISQESDQTQTTLLELTKLIADLEKQIELVKLESGQEAEKKAEAKKHLEQLQEQLDG
                     FQAEEKQRTEQLLHIDQQLCDVKQQLNELSNALERFSSDPDQLMETLREEFVLLMQKE
                     AALSNQLTALKAHLDKEKQARQHKAQEYQLLVTKLDQLNDESQKAQAHYKAQKEQVEM
                     LLQNYQEGDKRVQELERDYQLKQERLFDLLDQKKGKEARKASLESIQKSHSQFYAGVR
                     AVLQSQKKLGGIIGAVSEHLSFDSDYQTALEIALGANSQHIIVTDEAAAKRAIAYLKK
                     NRQGRATFLPLTTIKARSLSEHYHRQLATCEGYLGTAESLVRYDDSLSAIIQNLLSST
                     AIFETIDQANIAARLLGYKVRIVTLDGTELRPGGSFSGGTNRQSNTTFIKPELEQISE
                     ELTRLVEQLKITEKEVAALQSDLIAKKEELTQLKLAGDQARLAEQRAQMAYQQLQEKQ
                     EDSKALLAALDQSQTTHSDESLLAEQARIEEALTAIAKKKNALTCDIDDIKENKDLIR
                     QKTQNIHQALSQARLQERDLLNEKKFEQANQSRLRTQLKQCQQNILKLESILNNNVSQ
                     DSIQRLPQWQKQLQDATEHKSGAQKRLVQLRFEIEDYEARLEETAEKITKESEKNDTF
                     IRRQTKLETHLEQVANRLRAYAKSLSEDFQMTLADAKEVTNSIDHLESAKEKLHHLQK
                     TIRALGPINSDAIIQYEEVHERLTFLTSQKTDLTKAKNLLLETINSMDSEVKARFKVT
                     FEAIQKSFKETFTQMFGGGSADLVLTETDLLSAGIEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDFLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGITMQESGVSKIVSVKLKEAQEMTN"
     gene            complement(5192..5884)
                     /locus_tag="VHD02_000527"
     CDS             complement(5192..5884)
                     /locus_tag="VHD02_000527"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEQ5497159.1"
                     /translation="MKQLEELLSTSFDIQFNDLTLLETAFTHTSYANEHRLLNVSHNE
                     RLEFLGDAVLQLIISEYLFAKYPKKTEGDMSKLRSMIVREESLAGFSRFCSFDAYIKL
                     GKGEEKSGGRRRDTILGDLFEAFLGALLLDKGIDAVRRFLKQVMIPQVEKGNFERVKD
                     YKTCLQEFLQTKGDVAIDYQVISEKGPAHAKQFEVSIVVNGAVLSKGLGKSKKLAEQD
                     AAKNALAQLSEV"
     gene            complement(6316..7125)
                     /locus_tag="VHD02_000528"
     CDS             complement(6316..7125)
                     /locus_tag="VHD02_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEQ5497160.1"
                     /translation="MNESGFKYSILASGSTGNCFYLETPKKRLLIDAGLTGKKITSLL
                     AEIDRKPEDLDAILITHEHSDHIKGVGVMARKYHLDIYANEKTWQLMDECNMLGKLDA
                     SQKHIFQRDKVLTFGDVDIESFGVSHDAIDPQFYRIMKDNKSFVMLTDTGYVSDRMTG
                     IIENADGYLIESNHDIEILRSGSYPWSLKQRILSDLGHLSNEDGAGAMIRSLGYNTKK
                     IYLGHLSKENNIKELAHMTMVNQLAMADLAVGTDFTVHDTSPDTACPLTDI"
     gene            complement(7129..8481)
                     /gene="vicK"
                     /locus_tag="VHD02_000529"
     CDS             complement(7129..8481)
                     /gene="vicK"
                     /locus_tag="VHD02_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEQ5497161.1"
                     /translation="MTRDIIGNLSTFELAILILLVFVAFYFIHLAVRDYRNARIIRMM
                     SHKIRDLINGRYTDIIDEKADIELMELSDQLNDLSDVFRLTHENLAQEKNRLASILAY
                     MSDGVLATDRSGKIIMINETARKQLNLSKEEALKKNITDLLEGDTSYTYRDLVSKTPV
                     VTVNSRNDMGEFVSLRLRFALNRRESGFISGLVVVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALKEDIAPSFIKVSLDETNRMMRMISDLLNLSRIDNQVT
                     QLAVEMTNFTAFITSILNRFDLVKNQHTGTGKVYEIVRDYPITSVWLEIDNDKMTQVI
                     ENILNNAIKYSPDGGKITVRMKTTDTQLIISISDQGLGIPKTDLPLIFDRFYRVDKAR
                     SRAQGGTGLGLAIAKEIIKQHHGFIWAKSDYGKGSTFTIVLPYEKDAAIYEEWEEDVD
                     "
     gene            complement(8474..9184)
                     /locus_tag="VHD02_000530"
     CDS             complement(8474..9184)
                     /locus_tag="VHD02_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ5497162.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYDIVTAFDGREAVTIFEEE
                     KPDLIILDLMLPELDGLEVAKEIRKTSHVPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTETIETAVAEENASSGTQELTIGNLQILPDAFVAKKHGQEVE
                     LTHREFELLHHLANHMGQVMTREHLLEIVWGYDYFGDVRTVDVTVRRLREKIEDTPSR
                     PEYILTRRGVGYYMKSYD"
     gene            complement(9346..10380)
                     /locus_tag="VHD02_000531"
     CDS             complement(9346..10380)
                     /locus_tag="VHD02_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEQ5497163.1"
                     /translation="MCYNMVKKDEEKAMKQAILDRYQALKCYQNAGLSNQAFRAIAKE
                     PMIDNRLGSPTFWVIWPIEKENQSAKQLLTFLLDLVEMPFELSGQLHETQTLLTRFHP
                     SLLPDHMFWKELASLVDQAFPGKTLSQAGELEKRLHQFRYVISSQQAQSIRNHYKMIE
                     MTDAQALALFLRSKKGPCLWRQAPDYTLMDSARLHNKLRFEDNKVIFPSQEVSYNIKV
                     LLWFHTEFILDSTGFFLNEVDAEVVTEKGIVNGASFNYGTDGPRHWDLDVDPISRHDP
                     QFRRDTLKGFRSPKRVFRQWFRAQKDDFMFSYFNAKGLFAYHNKSSFARVKKSAKQFK
                     RQIHPIKGWF"
     gene            complement(10436..11683)
                     /locus_tag="VHD02_000532"
     CDS             complement(10436..11683)
                     /locus_tag="VHD02_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEQ5497164.1"
                     /translation="MLTKLEYWAKQCPNKKAIVADQISLTYQELWQAVLIKDQTIKDS
                     VPYIISHSRYLNQLLSFLRGLKEGSCPIILHPNISGTFQQQIKHVDGELLEKADFAVL
                     SSGTTGKAKLFWRCLSTWTRLFDYQNKMFGMTGNSCLFLHGSFSFTGNLNLALAQLWA
                     GGCLVLSQKLSLKTWLSLWQAKKVSHLYLLPTYLNRLLPYLTKNNMTATHLLTSSQMI
                     SQELLRHYYKKFPQLEIVIFYGASELSFITWCNGRAAVKINGLVGQPFPDVSISFKDK
                     EIFVETPYSVEGMSQPYSVSDLGKMSPAGLILEGRQDDWVNQRGVKCHLPSLVELAHQ
                     ALNVKEAHALKIGKGENETLILVLVLTKKDCLAPIKDFLALYLNSGQLPKYYLVIDCL
                     PLKDNGKINREVLLNKIPKQWLS"
     gene            complement(11640..12788)
                     /locus_tag="VHD02_000533"
     CDS             complement(11640..12788)
                     /locus_tag="VHD02_000533"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEQ5497165.1"
                     /translation="MTDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVP
                     IDQVICGNAVGTGGNIGRLMTLYSHLGESVSALTVDMQCASAGAALSVGYAKIKAGMA
                     SNLLVGGIESSSLQPESVYASADWRQGAYKVAQFSPDSISPFAMIEGAERVAREHGFT
                     KEYLNHWILRSHQKASYCQEQALLADLILDLPGASDQGIRPRLSSKVLSKVPPILGED
                     HVISAANACLTHDAAAFLQLSSQPSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHG
                     LGMADMTAIEWNEAFAVIDGLFETHYPDLLDRYNIFGGALAYGHPYGASAAIIILHLM
                     RALEIKNGHYGIAAIAAAGGQGFAVLLKYHKEFSHADKARILGKAMPK"
     gene            complement(13183..13332)
                     /locus_tag="VHD02_000534"
     CDS             complement(13183..13332)
                     /locus_tag="VHD02_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase"
                     /protein_id="HEQ5497166.1"
                     /translation="MQLLDNVYGTLRSFAFMRPVKSSNDTSLADALQADTDMAFILVR
                     GINYQ"
     gene            complement(13418..14203)
                     /locus_tag="VHD02_000535"
     CDS             complement(13418..14203)
                     /locus_tag="VHD02_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ5497167.1"
                     /translation="MAKLRRMHAIFIKQYLKQIMEYKVDFVVGVLGVFLTQGLNLLFL
                     SVLFQHIPSLEGWTFEQIAFIYGFSLIPKGIDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGVILLVTTAGSIVWTLPKVLLFILVIPFATLIYTS
                     LKIATASISFWTKQSGAVIYIFYMFNDFSKYPMSIYHSFLRWLISFVIPFAFTAYYPA
                     SYFLTGQHLLFNIGGLVVVSLLVLALSLKLWKWGLDAYESAGS"
     gene            complement(14205..15023)
                     /locus_tag="VHD02_000536"
     CDS             complement(14205..15023)
                     /locus_tag="VHD02_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEQ5497168.1"
                     /translation="MWSFWKRYRPFLSAGIQELITYRVNFFLYRIGDVMGAFVAYYLW
                     KAVFDSSKQSLINGFTLSDMTFYIIMSFVTTLLTKSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFMEIGFRWIVLMSVGFPFLVVLSGIKVMAGLSILQVLASSCLYLVSLLLA
                     FLINFYFNICFGFSAFVFKNLWGSNLLKNALVAFMSGSLIPLAFFPKMVSIVLSFLPF
                     SSLVYTPVMIVIGKYSLSQIMVALSLQIFWLLVMVVLSQVIWKKVQYHLTIQGG"
     gene            complement(15025..16017)
                     /locus_tag="VHD02_000537"
     CDS             complement(15025..16017)
                     /locus_tag="VHD02_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ5497169.1"
                     /translation="MVMIEVSHLQKNFSKTIKEPGLKGALKSFVHPQREIFEAVKDLS
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGYCRINGKIPQDNRQDYVRDIGAV
                     FGQRTQLWWDLALQETYVVLKEIYDVPEKAFRKRMDFLNEVLDLNEFIKDPVRILSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIIMIDKGQEIFDGTVTQLKQSFGKMKSLSFELRPGQEQVVSQFMGLPDIT
                     VERHELSLDIQYDSSRYQTADIIQKTMADFAVRDLKMTDVDIEDIVRRFYRKEL"
     gene            complement(16158..18101)
                     /gene="thrS"
                     /locus_tag="VHD02_000538"
     CDS             complement(16158..18101)
                     /gene="thrS"
                     /locus_tag="VHD02_000538"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEQ5497170.1"
                     /translation="MIKITFPDGAVREFESGVTTFDIAESISKSLAKKALAGKFNDQL
                     IDTTRAIEEDGSIEIVTPDHKDAYEVLRHSAAHLFAQAAKRLFPNLHLGVGPAIAEGF
                     YYDTDNAEGQISNEDLPRIEAEMQKIVTENYPCIREEVTKEEALELFKDDPYKVELIN
                     EHAGAGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNNMMQRIYGT
                     AWFDKKDLKAYLTRLEEAKERDHRKLGKELDLFMISQEVGQGLPFWLPDGATIRRTLE
                     RYITDKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPVMDMGDGEEFVLRPMNC
                     PHHIQVYKNHVRSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHIFVTPEQ
                     IQEEFQRALQLIIDVYADFNLTDYRFRLSYRDPNDTHKYYDNDEMWENAQSMLKAALD
                     EMGVDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPVMIHRGVISTMERFTAILIETYKGAFPTWLAPHQVTVIPISNEAHIDYAWEVAKT
                     LRDRGVRADVDDRNEKMQYKIRASQTSKIPYQLIVGDKEMEDKSVNVRRYGSKATHTE
                     SVEEFVENILADIARKSRPDAQA"
     gene            complement(18523..19857)
                     /locus_tag="VHD02_000539"
     CDS             complement(18523..19857)
                     /locus_tag="VHD02_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEQ5497171.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKEELEKEGHEVYIFTTTDRD
                     VKRFEDPTIIRLPSVPFVSFTDRRVVYRGLISSYKIAKHYNLDIIHTQTEFSLGLLGK
                     MIGKALRIPVVHTYHTQYEDYVSYIANGKIIRPSMVKPLLRGYLKDLDGVICPSRIVL
                     NLLEGYEVTIPKRVIPTGIPLEKYIRDDITAEEVTNLKAELGIAGDETMLLSLSRISY
                     EKNIQAIINQMPAILAENAKIKLIIVGNGPYLQDLKHLAMQLEVDKHVTFTGMVPHDK
                     VALYYKACDFFISASTSETQGLTYIESLASGTPIIAHGNPYLDDVVTDKMFGTLYYAE
                     TDLTDAVIDAILKTPVMDKRLLAKKRYEISAQHFGKSIYTFYLDTLIARNSKEAQKLS
                     LYLNHSGKSSSLKLVQGAIHLPKRAAKVTAITSVKVVKAPIKLVHAIKDFLD"
     gene            complement(19859..20857)
                     /locus_tag="VHD02_000540"
     CDS             complement(19859..20857)
                     /locus_tag="VHD02_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEQ5497172.1"
                     /translation="MKVLLYLEAENYLRKSGIGRAIKHQAKALSLVGQHFTTNPRETY
                     DLVHLNTYGLKSWLLMIKAQKAGKKVIMHGHSTEEDFRNSFIFSNLLSPWFKKYLCHF
                     YNKADAIITPTLYSKSLIESYGVKSPIFAVSNGIDLEQYGADSKKEAAFRRYFDIKEG
                     EKVVMGAGLFFLRKGIDDFVKVAQAMPDVRFIWFGETNKWVIPAQVRQMVNGNHPKNL
                     IFPGYIKGDVYEGAMTGADAFFFPSREETEGIVVLEALASRQHLVLRDIPVYYGWVDQ
                     SSAELATDIPGFIEALKKVFSGASNKVEAGYKVAQSRRLETVGHALVDVYKKVMEL"
     gene            complement(20988..21989)
                     /gene="ccpA"
                     /locus_tag="VHD02_000541"
     CDS             complement(20988..21989)
                     /gene="ccpA"
                     /locus_tag="VHD02_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEQ5497173.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILAKGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPVVLAGTVDLDHQLPS
                     VNIDYRAAVSNVVDILAENHKCIAFVSGPLIDDINGKVRLAGYKEGLKHNKLDFKEGL
                     VFEANYSYKEGFELAQRVINSGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPVVQYTRPNLSSISQPVYDLGAVSMRMLTKIMNKEELEEKEILLNHGIKKRGTTK"
     gene            22163..23248
                     /locus_tag="VHD02_000542"
     CDS             22163..23248
                     /locus_tag="VHD02_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEQ5497174.1"
                     /translation="MTKLDQIRLYLDQKGAELAIFSDPVTINYLTGFFCDPHERQLFL
                     FVYHDLAPVLFVPALEVARASQAISFPVFRYVDSENPWEKIKAVLPNTAAKTIYAEFD
                     HLNVNKFHGLQTIFSGQFNNLTSYVQGMRLVKSADEINKMMIAGQFADKAVQVGFDNI
                     SLDATETDVIAQIEFEMKKQGIHKMSFDTMVLTGNNAANPHGIPGTNNIENNALLLFD
                     LGVETLGYTSDMTRTVAVGQPDQFKIDIYNLCLEAQLAAIDFIKPGVTAAQVDAAARQ
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMAGNDLVLEEGMCFSVEPGIYIPGKVGVR
                     IEDCGHVTKNGFEVFTHTPKELLYFEG"
     gene            23297..23962
                     /locus_tag="VHD02_000543"
     CDS             23297..23962
                     /locus_tag="VHD02_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEQ5497175.1"
                     /translation="MDQTIHHQIQQALHFRTAVRVYKEEKISDEDLALILDAAWLSPS
                     SVGLEGWRFVVLDNKPIKEEIKPFAWGAQYQLETASHFILLIAEKHARYDSPAIKNSL
                     LRRGIKEGDGLNSRLKLYESFQKEDMDMADNPRALFDWTAKQTYIALGNMMMTAALLG
                     IDTCPIEGFHYDKVNHILAKHNVIDLEKEGIASMLSLGYRLRDPKHAQVRKPKEEVIS
                     VVK"
     gene            23973..24350
                     /locus_tag="VHD02_000544"
     CDS             23973..24350
                     /locus_tag="VHD02_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEQ5497176.1"
                     /translation="MKALHTCIRVKDLDQSVAFYTSAFPFKENYRKDFPDSQFTLVYL
                     ALEGESYELELTYNYGHGDYDLGNGYGHIALGSEHFEADHKKHRQAGFPVTDIKELAD
                     KSARYYFIQDPDGYKIEVIDLNN"
     gene            24495..25421
                     /locus_tag="VHD02_000545"
     CDS             24495..25421
                     /locus_tag="VHD02_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEQ5497177.1"
                     /translation="MTLLSIIVPCFNEEANILPYFEEMHQLETSMSNQLAFEYIFIDD
                     GSKDNTLGILRELAARFPNVHYLSFSRHFGKEAGLLAGLKEAKGNYITVMDVDLQDPP
                     ELLPVMYAKLKEGYDIVGTRRQNRQGEPLIRSMCSNLFYGLIKHLSDTEMVNGVRDYR
                     LMTRQVVDSILELGEVNRFSKGIFSWVGYRITYLSFENQKRKYGKSRWHFWELLRYSL
                     DGFINFSEMPLTIATWTGTFSFLISIFAILFIIIRKILFGDPVSGWASTVSIILFMGG
                     IQLFCMGIIGKYISKIFLETKKRPLYIIKEKH"
     gene            25632..26315
                     /locus_tag="VHD02_000546"
     CDS             25632..26315
                     /locus_tag="VHD02_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEQ5497178.1"
                     /translation="MEWIKLIGIVIIVLGFILKCDAIATVVVAGLVTALVSGISFIDF
                     LDILGKEFTNQRLLTIFFITLPLIGLSETYGLKHRATQLIQRVQALTVGRLLTLYLII
                     RELAGLFSIRLGGHPQFVRPLIQPMGEAAAKANIGEELTDAEKDDIKAMAAANENFGN
                     FFAQNTFVGAGGVLLIAGTLEQLGYDGNQAKIAFSSILIAIISIIIVAIYNYLFEKKM
                     ERQHQKGDN"
     gene            26315..27241
                     /locus_tag="VHD02_000547"
     CDS             26315..27241
                     /locus_tag="VHD02_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEQ5497179.1"
                     /translation="MANIANLVLETIYILIGLQLFHTAYCTFKDKTNPVYFGTALFWG
                     LLGVTFVGGAFLPNKVIGFIVIVLALLTLFKQVRIGTLPAFNEQKAEESAHRIGNWIF
                     LPVMLMAMISLLLALILPDFSKSAIGIAGILATIAILIITKQKPSALLAENNRMNQQV
                     STSGILPQLLGALGAIFAAAGVGDVIASLIREIVPADSRFFGVLAYVLGMVIFTMIMG
                     NAFAAFTVITTGIGVPFVFALGADPIIAGALAMTAGFCGTLLTPMAANFNALPVALME
                     IKDRNAVIKKQAPIALVLIISHIALMYLLAYY"
     gene            27291..27938
                     /gene="pcp"
                     /locus_tag="VHD02_000548"
     CDS             27291..27938
                     /gene="pcp"
                     /locus_tag="VHD02_000548"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEQ5497180.1"
                     /translation="MKILVTGFDPFGGEAINPALEAIKKLPATIHGAEIKYIEVPTVF
                     QKSADVLQQHIESFQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RADGKAAYFSTLPIKAMVAAIHQAGLPASVSNTAGTFVCNHLMYQALYLVDKYCPNAK
                     AGFMHIPFMMEQVVDKPNTAAMNLDDITRGIEAAIFAIVDFKDRSDLKRVGGATH"
     gene            complement(28053..28772)
                     /locus_tag="VHD02_000549"
     CDS             complement(28053..28772)
                     /locus_tag="VHD02_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEQ5497181.1"
                     /translation="MSIMITKGQVCLLRTYSYDSNLYTQGLEQLNNNHILLSAGRYGF
                     SKVGVYDLTQEIFSEKIAFPDTVFAEGLTVVEDYFWLLTYKEGVAYKFDKATCNCLGA
                     YPFEGDGWGLAYDKENQCLWMTSGNAFLQKRDPKDFALLDTVLVAIESVPISMLNELE
                     YVDGYLYANIWQTNTIVKLQPDSGKVVATYDISPLLKALNLDKSHYPDLNVLNGIAHL
                     DQQRFLITGKLYPLMLEVVLD"
     gene            complement(28778..29245)
                     /gene="smpB"
                     /locus_tag="VHD02_000550"
     CDS             complement(28778..29245)
                     /gene="smpB"
                     /locus_tag="VHD02_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEQ5497182.1"
                     /translation="MAKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARI
                     QLKDGFAQIKNGEAWLVNVHIAPFEQGNIWNADPERTRKLLLKKREITHLANELKGSG
                     MTLAPLKVYLKDGFAKVLIGLAKGKHEYDKRETIKRRDQERDIKKQMKHYNAR"
     gene            complement(29248..31581)
                     /gene="rnr"
                     /locus_tag="VHD02_000551"
     CDS             complement(29248..31581)
                     /gene="rnr"
                     /locus_tag="VHD02_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEQ5497183.1"
                     /translation="MMKERIMTYLKEHGKSNVNDLAAALEMAGAKHFPSLIKTISKME
                     SQSLLRFSDDGSLALRKEREKKKEPTVQGVFRANKAGFGFLHVDENEDDMFIGRNDVG
                     YAIDGDTVEVVVKKPADRLKGTAAEAKVVAIVDRSLKTAVGTFILDDDKPKYAGYIRS
                     KNQKIQQKIYIKKEPVVLKGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVL
                     EVLESMDIVSEFPAEVLAEANAISEAPTAKDLIGRVDLRQETTITIDGADAKDLDDAI
                     HIKLLDNGNYELGVHIADVSYYVTEGSALDKEAIARGTSVYVTDRVVPMLPERLSNGI
                     CSLNPNVDRLTQSALMEINSQGHVVNYQICQSVIKTTYRMTYSTVNDMIAGDEEALQE
                     FASIADDVTLMVALHRILEAMRSKRGALNFDTQEAKVIVNDKGIPVDVVLRQRGIAER
                     MIESFMLAANECVAEHFAKAKLPFIYRIHEEPKAEKLQQFIDYASTFGIHIQGTANKI
                     SQEALQAFMAKVEGQPGAEVLNMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRR
                     YPDLLVHRMVREYSQPSQEKRDHFTQIIPELATSSSQLERRAIDAERVVEAMKKAEYM
                     AEYVGEEFDGIVASVVKFGFFVELPNTIEGLVHITSLPEYYHFNERTLSLQGEKSGKV
                     FKVGQPIRVKLVKADKETGDIDFEYLPSDFDVVEKIKMSDKASRRDRRKSSKSSKGIK
                     KKEPKEVAKAKTKGKTKKGSKKPFYKEQAKKKSRKRS"
     gene            complement(31675..31911)
                     /gene="secG"
                     /locus_tag="VHD02_000552"
     CDS             complement(31675..31911)
                     /gene="secG"
                     /locus_tag="VHD02_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEQ5497184.1"
                     /translation="MYNLLLTILLVLSVLLVIAIFMQPQKNPSSNVFDSSGSEALFER
                     TKARGFEAFMQRFTAVLVFFWLAIALVLAILSSK"
     gene            complement(31957..32103)
                     /gene="rpmG"
                     /locus_tag="VHD02_000553"
     CDS             complement(31957..32103)
                     /gene="rpmG"
                     /locus_tag="VHD02_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEQ5497185.1"
                     /translation="MRVKINLECSECGSNNYLTSKNKSSHPEKIKVPKYCPKERKVTL
                     HVET"
     gene            complement(32100..33293)
                     /locus_tag="VHD02_000554"
     CDS             complement(32100..33293)
                     /locus_tag="VHD02_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEQ5497186.1"
                     /translation="MNWRQNLKVAWLGNFFTGASFSLVMPFMALYVENLGTPTELVEY
                     YAGLAVAVTALASALFAPVWGKLADRYGRKPMMLRASFVMTFTMGGLAIIPNVFWLLI
                     LRLLTGVFAGYVPNATALIASQAPKEESGYALGTLATGVTAGALIGPLLGGILAELLG
                     IRQVFLLVGVILFLCSLMTAVYVKEEFKPIRRFEMIPTKVILKQVKSPQIMLGLFVTS
                     MIIQISAQSVTPILSLYIRHLGQTHNLMFTSGLVVSAMGFSSLFSSSYLGKLGDRFGN
                     HRLLLAALCYSFIMYFSSALAQTSFQLGVLRFAYGFGVGALMPSINSLLTKLTPKEGI
                     SRVFAYNQMFSNLGQVIGPFIGSNVAVVLGYRSVFYVTSLIVFVNLIWSLIIFRKYIK
                     VKDIV"
     gene            complement(33415..34917)
                     /locus_tag="VHD02_000555"
     CDS             complement(33415..34917)
                     /locus_tag="VHD02_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HEQ5497187.1"
                     /translation="MTTMTIAKGSQPIALQLGMLNRHGLIAGATGTGKTVTLKVLAEQ
                     LSLAGVPVFLADIKGDLSNLTKAGEVTDKLAARLATIGVSDYQPQAFPVRMWDVFGQN
                     GQPLRTTISEMGPMMLSRLLNLNDTQTGVLNIVFKIADEKGWLLIDLKDLQAILKEVG
                     DHASDYSSHYGNIAKQSIGAIQRSLLTLEQEGAHQFFGEPALDVADLMQLDVASGYGA
                     INILSATKLFQSPTLYTTFLLWLLSELYELLPEVGDLDKPKMVFFFDEAHLLFKDAPK
                     VFLEKVEQIVRLIRSKGVGIFFVTQNPLDLPETVLAQLGNRIQHALRAYTPKEQKAVR
                     VAADTFRQNPDLDVARVITELEVGEALISVLNDKGQPSIVERAYIMPPKSSFAVLSDI
                     ESQQLVQSSPFASKYSQSIDRDSAYEKLAAKVLEDNRLAQEAIATAQREKEAKEAIKA
                     QAATKKANRRSVGRPRKTVVEKATDAFISTTVRTIGRELVRGLLGSLRKR"
     gene            complement(35107..35700)
                     /locus_tag="VHD02_000556"
     CDS             complement(35107..35700)
                     /locus_tag="VHD02_000556"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEQ5497188.1"
                     /translation="MIIGITGGIASGKSTVVKVIRKAGYQVIDADQVVHDLQEKGGRL
                     YEALREAFGNQILKADGELDRTKLSEMLFSNPNNMATSSAIQNQIIKEELAAKRDHLA
                     QSQAIFFMDIPLLMELGYQDWFDAIWLVYVDAQTQLQRLMARNRLDKGKARQRIASQL
                     PIEEKKPYASLVIDNNGDMETLIKQVQSALLSLANPR"
     gene            complement(35697..36524)
                     /gene="mutM"
                     /locus_tag="VHD02_000557"
     CDS             complement(35697..36524)
                     /gene="mutM"
                     /locus_tag="VHD02_000557"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEQ5497189.1"
                     /translation="MPELPEVETVRRGLETLVLGQEIVAVTLKVPKMVKTDLETFALT
                     LPGQIIQSVGRRGKYLLIDLGQLVLVSHLRMEGKYLLFPDEVPDNKHFHVFFELKNGS
                     TLVYQDVRKFGTFDLIAKSQLSAFFAKRKLGPEPKKETFKLKTFEAALLSSQKPIKPH
                     LLDQTLVAGLGNIYVDEVLWAAKVHPETASSRLNKAEIKRLHDETIRILALGIEKGGS
                     TVRTYRNALGADGTMQDYLQVYGQTGKPCPRCGQAIVKLKVGGRGTHICPKCQKKRP"
     gene            complement(36696..37562)
                     /gene="rgg2"
                     /locus_tag="VHD02_000558"
     CDS             complement(36696..37562)
                     /gene="rgg2"
                     /locus_tag="VHD02_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg2"
                     /protein_id="HEQ5497190.1"
                     /translation="MEKELGKTLRRLRKGKQVSISFLTDEYLSKSQISRFERGESEIT
                     CSRLLNLLDKLNITIDEFVSAHSKTHTHFFTLLSQARKCYAEKNVVKLTKLLKDYAHK
                     DYERTMIKAILFSIDSSIAPSQEELTRLTDYLFKVEQWGYYEIILLGNCSRFMNYNTL
                     FLLTKEMVASFAYSEQNKTNKMLVTQLSINCLIISIDHSCFEHSRYLINKIDLLLRDE
                     LNFYEKTVFLYVHGYYKLKQEEMSGEEDMRQALQIFKYLGEDSLYYSYKEHYRQIVLG
                     GKGDEDWSEADL"
     gene            37651..37719
                     /gene="shp2"
                     /locus_tag="VHD02_000559"
     CDS             37651..37719
                     /gene="shp2"
                     /locus_tag="VHD02_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEQ5497191.1"
                     /translation="MKKVNKALLFTLIMDILIIVGG"
     gene            38633..38902
                     /gene="stcA"
                     /locus_tag="VHD02_000560"
     CDS             38633..38902
                     /gene="stcA"
                     /locus_tag="VHD02_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system protein StcA"
                     /protein_id="HEQ5497192.1"
                     /translation="MTKKQFYLILAISILCFILAVSPKPPIPKHTIERKRTTMTIGHS
                     KQNPHKKEALKHKKNKNKALFLPSGDSAIKSVISMTVRLLLDDQS"
     gene            complement(39250..39648)
                     /locus_tag="VHD02_000561"
     CDS             complement(39250..39648)
                     /locus_tag="VHD02_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEQ5497193.1"
                     /translation="MHRLLNRLIPEEDNNKLSRAAKEVVTEPRLNNPFLLPKKVLSLE
                     DDAAILNHLEEDRNVNKLFLEPVAAEAKREINFHPSDQQVTAKVKESSDFHQKQHQTG
                     ITYGDDTLADLAHHLLHSDGVIPSLNDKSA"
     gene            complement(39741..40811)
                     /locus_tag="VHD02_000562"
     CDS             complement(39741..40811)
                     /locus_tag="VHD02_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497194.1"
                     /translation="MMMLRDVKVKTSSLLVGCAATLLVSLSSTVAADSVHSSDRRFGV
                     GYKEETTSQFSSDQPSQVVLYRGEANTVRFTYTNQMYLMKDIRIALDGSGKSLTAQTV
                     PGMGHVYEGFQTSARGIFTMSGVPESTVPVANPNVQTKYTRYFKVIDDMHNTMYKGTV
                     FLVQPQAWKYTMKSVDQLPVDDLNHIGVAGIERMTTLIKNAGALLTTGGSGAFPDNIK
                     VSINPKGRQATITYGDGSTDIIPPAVLWKKGSVKEPTEADQSVGTPTPGIPGKFKRDQ
                     SLNEHEAMVNVEPLSHVVKDNIKVIDEKSTGRFEPFRPNEDEKEKPASDVKVRPAEVG
                     SWLEPATALPSVEMSAEDRLKS"
BASE COUNT        12431 a         8736 c         7199 g        12817 t
ORIGIN      
        1 tataaatatt gttaacagat ccagttatct ctgctactct gtctaaattg tcaatatacc
       61 tcattacctc tcttttataa ggtaaagtta tatttaatcc aatacatttt ggatcattta
      121 tcagatgttt gattttatct tctaaacaca ctgagtctac caaaattttt ttatgtttat
      181 attcatccag aactcctatg ttgtgattta gatattcaaa taattcatct gatatagaat
      241 gttcaactgg attaccaagc aatgcagaat acataaattt catcctccta cgtttaatta
      301 gtttactact aacataaagt acaagcatta cacagagcta accattacct ataagtcttt
      361 agagtatcta aagacttagg tatacaccta agtaacttac ccaccaacaa taatgataat
      421 atccattgct aaaattaaaa tcggcaaaaa ttttgaaatt ttcttcataa gtggacttcc
      481 tttcagtttt tatttattgt acaataaaaa aaaacggaat tgatttttgt tgtgaaagtg
      541 ggaaaatatg aaatcaaaac ttggtgccac acttagaaaa gttcgaaaag gaaagcaaat
      601 tagtctgtgc ttcgtggcag atgaacacct ttcaaaatca caaatctctc gttttgagcg
      661 cggggagtca gagatatcgt gtatccgact gatcaatatt ctagacaaat tacacattac
      721 tttggatgag ttccttattt tacatgatga agactatacc aattccgaaa catttgctaa
      781 cctggtacag tatattcgta agcaatattc atcacaaaat atcaataata tagcatgttt
      841 actttccgac acttctgatt acactctgaa ttcttttgag aaaacaatgg taaagtccat
      901 tttacacaca atggattcaa gcatcattcc atcagatgat gaattacttc aactaacaga
      961 ttatctcttt aaagttgaaa aatggggcta ctatgaaatc cttttattgg gtaactgtgt
     1021 acgaacaatt aactacaact cctacttcct attgacaaag gagatgttaa ataattacat
     1081 ttactcttca cggaacaaaa ctaataaacg aattgtcact cagcttgcta taaattgttt
     1141 cattcttagc atcaataagg aggaattttc taattgctcc tatttaattg atgagataaa
     1201 aaccttgtta gataatgaat taaacttcta tgaacagaca gtttttctct atgcaacagg
     1261 ttactttgaa tttaagcgcc aattagaaac tggagttgaa aagatgaaac aagccataca
     1321 agttcttgat attttaaatg aagacaagtt aaaattacat tacgctgacc attttgataa
     1381 actagtcaac aaagattaag tgaaatcatt ctttaccttt taacgaaatg ccaatcaaga
     1441 gtgtctcaat aaaaaatcat ctactctgac aacttttctc ttttacctat cgacagctaa
     1501 aaagccttgg aaactgctat ttccaaggct ttctttgtac tctattgtat caattagctt
     1561 attccgattt ctgttgctat tagtttaatc atgaaatctc ctaacttatg gccgaacatc
     1621 caaaaaaaca gaaggaactg ccttctgttt tttaattggt catctcttga gcttctttta
     1681 attttacaga tacaatttta gagacccccg attcctgcat ggtaattcca taaatactat
     1741 ctgctgcagc cattgttcct ttacgatggg tcacgacaat aaattgacta tctttgtcaa
     1801 agcgattgag gaaatcccca aagcgcttaa catttgcttc atcaagagcc gcttctacct
     1861 catctaaaat cacaaacgga atcgttttaa cacgtataat agcaaacaat aaagccagtg
     1921 ctgacaaagc tttttctccg cctgacatca agttaagaga ttggattttc ttaccaggag
     1981 gctgcacaga aatctctata ccagcagaca agagatctgt ttccgttaaa accaaatcag
     2041 cagaaccccc accaaacatc tgagtaaagg tttccttaaa acttttttga attgcctcaa
     2101 aagtcacttt gaaacgagcc ttcacttcgc tatccatact attaatagtc tctagcaaaa
     2161 gatttttagc ttttgtcaga tctgtctttt gacttgttaa aaaagtcaaa cgctcatgca
     2221 cttcctcata ctgaataata gcatcactat taattggtcc tagtgcacga atagtctttt
     2281 gtaaatggtg taatttttct ttggcacttt ctaagtgatc aatactattt gttacctcct
     2341 ttgcgtctgc tagtgtcatt tgaaaatctt ctgataaact tttagcatag gctcgtaagc
     2401 gattagccac ttgctctaaa tgtgtctcta acttggtttg acgacgaata aatgtgtcat
     2461 ttttttcgct ttctttggtt attttttctg ctgtttcttc taagcgggcc tcatagtcct
     2521 ctatttcaaa tcttaattgg actaaacgtt tttgagcacc agacttatgc tctgtggcat
     2581 cttgtaactg tttttgccac tggggtagtc tttgaatgct gtcttgactt acgttgttgt
     2641 ttagtatgct ttcaagtttg agaatatttt gctggcattg cttcagctga gttcttagac
     2701 gagattgatt agcttgctca aatttctttt cattaagtaa atctctttct tgcaaacgag
     2761 cttgagataa agcttggtga atgttttgtg ttttttgcct aatcaaatct ttgttttctt
     2821 tgatatcatc aatatcacaa gttaaggcat tttttttctt agcaatagct gttaatgctt
     2881 cttcaatacg agcttgttct gccaataaag attcatcaga atgagtcgtc tgactttgat
     2941 caagggcagc taacaaagcc tttgaatctt cttgtttttc ttgcaattgc tgataagcca
     3001 tttgagctct ttgttcagct aagcgtgcct ggtcaccggc taatttcaat tgtgtcagtt
     3061 cttctttttt tgctatcaaa tcgctttgca aggcagctac ttctttttca gttattttta
     3121 attgctcaac taatcgcgtt aattcctcac tgatttgttc taactctggt ttgataaaag
     3181 ttgtattgct ttgtctatta gttcctcctg aaaaagaacc acctggtcgt aattctgttc
     3241 catcaagagt aacaattctt accttatatc ctaataagcg cgctgcaata ttagcttggt
     3301 caatagtttc aaagatagca gtactagata aaaggttttg gataatagct gaaagactat
     3361 catcatagcg aaccaatgat tctgcagttc ccagataacc ttcacaagtc gctaattgac
     3421 gatgatagtg ttctgataat gatctagcct ttatagtagt taaaggtaaa aaagttgctc
     3481 gtccttggcg attttttttc aaataagcaa ttgctctttt tgctgcagct tcgtctgtta
     3541 caataatatg ctgactatta gcacctaatg caatttcaag agctgtttgg taatcagaat
     3601 caaaagaaag gtgttcactg actgcaccta tgatccctcc aagctttttt tgtgattgaa
     3661 ggacagctcg tactcccgca tagaattgac tatgactttt ttgaatagat tctaagctag
     3721 ccttacgagc ttcttttcct tttttttgat ccaacaagtc aaatagtctt tcttgcttca
     3781 gttggtaatc tctttctaat tcctgaacgc gtttatcacc ttcttgataa ttttggagca
     3841 acatttcgac ttgctctttt tgagctttat aatgagcttg agctttttga gactcgtcat
     3901 taagttgatc aagcttagta acaagcagct gatattcttg agccttatgt tgacgagcct
     3961 gtttttcttt gtctaaatgg gcttttaaag ctgtcagctg atttgataag gccgcctctt
     4021 tttgcatcaa taatacaaac tcttctctta gagtttccat taattggtcg ggatcacttg
     4081 aaaaacgctc taatgcattg ctcaattcgt ttaactgttg tttgacatca caaagttgtt
     4141 gatcaatatg caataattgt tctgtacgct gcttttcttc tgcttgaaaa ccatccaatt
     4201 gttcttgtaa ctgttcgagg tgttttttgg cttctgcctt tttctctgct tcttggccac
     4261 tttctagctt aaccaattca atttgctttt ccaagtcagc tattagcttg gttaattcta
     4321 ataatgttgt ttgggtttga tctgactctt gactaataac ctgctttttt tgcttaaatt
     4381 tttggtaatc ttcttccata gattgacgct tagcgtaata agatgctaag tcttgttgta
     4441 aagcagctaa agcttcagta tctttagtct gtctttcttg agctatatca atatctttga
     4501 ctagaatatc tagttgtagt tgcttacgat tggcatcaag ttctaaaaat tgtttagcta
     4561 ctttagcttg cttttcaagt ggggccaact gcgtgtctag ctcataaata atatcctcta
     4621 gacggtctaa attatcttga gtttgattta atttaatctg agtttctttt ttacgtgttt
     4681 tatattttaa aacaccagct gcttcttcaa aaatggcgcg cctttcttca ggtttactat
     4741 tgaaaatttc ctctacacga ccttgagaaa taatggagaa agaatcacga ccaagccctg
     4801 tatccataaa caaatcatga atatcacgga gccttacctt gcgaccatca atcagatagt
     4861 cactatctcc atttcggtaa atatgccttt cgacacggat ttctttcttg gcagttttta
     4921 taaaatgatc ggaattatca agaacaaccg ctaccttagc atagtttaga ggattacggt
     4981 tttgtgtccc agcaaaaatg acatctggca tcttgccccc acgcagattt ttagcacttg
     5041 attctcctaa agcccaacgt aaactttctg taatattact tttacctgaa ccatttggtc
     5101 ctacgactgc agttactccc ttatcaaatt caattttagt cttgtccgca aaggacttga
     5161 acccttccaa ctcaatttct tttaaaaaca tctaaacctc actaagttgg gcaagggcat
     5221 ttttagcagc atcttgctcc gctaattttt tagacttacc taaaccttta ctaaggacag
     5281 caccattaac aactatcgat acctcaaatt gtttagcgtg ggcaggtcct ttctcactaa
     5341 tgacttgata atcaatagcc acatcacctt ttgtttgcaa aaactcttga agacaagttt
     5401 tatagtcttt taccctttca aaattccctt tttccacctg aggaatcatg acttgtttca
     5461 agaagcgacg tactgcatca atccccttat ctaataaaag agcacctaaa aaggcttcaa
     5521 ataagtctcc taaaatagta tcacgacggc gaccacctga cttttcctca ccttttccta
     5581 gtttgatata agcgtcaaaa gaacaaaatc gtgaaaaacc tgccaagctt tcctcacgta
     5641 caatcatgga acgtaactta gacatatctc cttctgtctt tttaggatac tttgcaaata
     5701 aatactctga aataatcaat tgtagaacag cgtctcctaa aaattccaag cgttcattat
     5761 gtgaaacgtt taggagacga tgctcgttag cgtaagaggt gtgggtaaaa gctgtttcta
     5821 gtaaagtcaa atcattaaat tgaatatcaa aagatgttga gagtaactct tcaagctgtt
     5881 tcataaaaaa tccctttcat atttgtcagt agaccttatt tagtatacca aaaatccacc
     5941 tagaaaaccg actctaagtg gatttttcgt gatttattaa acatcttaaa tacccgtcaa
     6001 aggaaacttt ctcatacaat caaaagactc tacactatct gaggggaatg tcccactaca
     6061 gatagtatag agctattttg tttttagtct gaacaactaa aaaactacct tatctttata
     6121 atcctcaaca cctttgataa atagatcaga caagccaaaa ctacaggcta atttaagtct
     6181 attgatgctc taaaggataa ccaatgagag ttcattgaac tcagaagaca atacgcttta
     6241 agacatcaaa aaatagccct gactggtata tcaccaatca gcgctacttt gcatattctc
     6301 ttttagtttc ttatctcaaa tatcagttaa tggacaagca gtatctggag aggtatcatg
     6361 gaccgtaaag tctgtaccta ctgctaaatc tgccatagcc agttgattga ccatcgtcat
     6421 atgcgctaac tctttgatgt tattttcttt actcagatga cctaggtata tttttttggt
     6481 attgtacccc aggcttctaa tcatggctcc cgctccatct tcatttgata aatgtcctaa
     6541 atcagataaa atacgctgct tcaagctcca aggataagag cctgatctca aaatttcaat
     6601 atcatgattg gattcaatca agtagccatc tgcattttca ataatacctg tcatcctatc
     6661 actgacatat cctgtatctg tcaacatcac aaaagactta ttatctttca taatccgata
     6721 aaattgagga tcaatagcgt catggctgac tccaaaactt tcgatatcaa catctccaaa
     6781 ggtcaaaact ttatcccttt ggaaaatatg tttttgtgag gcatcaagct tacctagcat
     6841 attgcactca tccatcagct gccatgtttt ttcattagca tagatatcca aatggtattt
     6901 gcgggccata acgcctacac ccttaatatg gtcagagtgc tcatgggtaa tcaaaatagc
     6961 atccaaatcc tcaggtttgc ggtcaatttc agcaagaaga ctggtaattt tcttaccagt
     7021 aagccctgca tcaatcaata agcgtttttt aggtgtttct aaataaaaac aatttcctgt
     7081 ggacccagat gctaaaatac tatatttaaa accactctca ttcattgatt agtctacatc
     7141 ttcctcccat tcttcataga tggctgcatc tttttcataa ggcaagacaa tagtaaaggt
     7201 cgatccttta ccatagtcac tcttagccca gataaagcca tggtgctgct tgatgatttc
     7261 tttagcaatg gcaaggccta gaccggtccc tccttgggca cgacttcttg ccttgtctac
     7321 acgatagaac cgatcaaaaa taagaggcaa atctgtttta gggataccta gtccttggtc
     7381 tgaaatggaa ataattaatt gggtatctgt tgttttcata cggactgtga tttttccacc
     7441 atctggagaa tacttaatgg cattgttcaa aatattctcg ataacctgtg tcattttatc
     7501 attatcaatt tcaagccaga cagaggtaat agggtaatct cttacaattt catagacttt
     7561 tcctgtacct gtatgctgat ttttaaccaa atcaaatctg tttaaaatag aagttataaa
     7621 agcagtaaaa ttagtcatct ctactgctaa ttgggttact tgattatcaa tccgagacag
     7681 gtttaaaaga tctgaaatca tacgcatcat ccgattagtt tcatcaagag aaacttttat
     7741 gaaacttgga gcaatatctt ctttaagtgc accttcatca agagcctcca agtaggattt
     7801 aaccgaagtt aaaggggtcc ttaattcatg acttacatta gagacaaaaa gacggcgttc
     7861 acgttcttct ttttcttgtt ctgtggtgtc atggagcacc acaaccaagc ccgaaataaa
     7921 accactctct ctcctattca acgcaaagcg caagcgtaat gagacaaact cacccatatc
     7981 atttcggcta ttaacagtta ccactggtgt tttggatacc aaatcacggt aggtatatga
     8041 agtatcacct tctaacaaat ctgtaatgtt tttctttagt gcctcttctt tacttaaatt
     8101 taattgcttg cgagctgtct cgttaatcat gatgatttta ccagaccggt ctgtagcaag
     8161 tacaccatct gacatataag ccaaaatact tgccaagcga tttttttctt gggcaagatt
     8221 ttcatgcgtc aagcgaaaaa catctgacag gtcatttaac tggtctgaaa gctccattaa
     8281 ctcaatgtct gctttttcgt cgattatatc agtatagcga ccattaatca agtctcggat
     8341 tttatggctc atcatccgaa taatacgtgc atttcggtaa tcacgcaccg caagatggat
     8401 aaagtaaaaa gcaacaaaaa caagtaagat aaggatcgct aattcaaatg tggataagtt
     8461 tccaatgata tctctagtca taagatttca tgtagtaccc aacaccacgt cttgttaaaa
     8521 tatactcagg acgacttggt gtgtcttcaa ttttttcacg gagacgacga acagtcacat
     8581 caaccgtgcg cacatcgcca aaataatcat atccccaaac aatttccaat aagtgttctc
     8641 gtgtcatcac ctgacccata tggttagcta gatgatgcaa tagttcaaat tcacgatggg
     8701 tcaactctac ctcttgacca tgttttttag caacaaacgc atctggtaaa atctgtaaat
     8761 taccaatggt tagttcctgt gtacctgaag aagcattttc ttctgcaaca gccgtttcaa
     8821 tagtttcggt acgacgcaga tgagccttga cacgtgccag caattcccga ttagaaaagg
     8881 gcttggtcac gtaatcatca gccccaattt caagtccaat aaccttgtca aactcactat
     8941 ctttagccga caacataata atcgggacat gactggtttt acgaatttcc ttggctactt
     9001 caagaccgtc caactcaggg agcatcaaat caagaataat taaatctggc ttttcttctt
     9061 caaaaattgt taccgcttcg cgtccatcaa aagctgtaac aatgtcataa ccttcttttg
     9121 tcaaattaaa cttaataatg tcagaaatcg gtttttcatc atccacaata agtatttttt
     9181 tcatgtctat acctcagaaa gttcttgtaa ttgatattac aatttgtgat ttttgtggtt
     9241 aataaaaaat cacaaaacta tatttttcta aattatttct acctgattat accaaaaatt
     9301 cagcctaatt gacaatgacg actaccgtta caaatagagt actttctaaa accaaccctt
     9361 tataggatga atttgacgtt tgaattgctt agcagatttt ttgactctgg caaaactact
     9421 cttgttgtgg taagcaaaaa gtcctttggc attaaaataa gagaacataa agtcatcttt
     9481 ttgagctcta aaccactgac gaaatactct ttttggcgat cgaaatcctt taagggtgtc
     9541 ccttctaaac tgaggatcgt gacggctaat cggatcaaca tctaaatccc aatgtctagg
     9601 accatctgtg ccatagttaa aagatgcacc attgacaatt cctttttcgg tcactacttc
     9661 tgcatcaact tcattaagaa aaaagcctgt gctatctaga ataaattcgg tatgaaacca
     9721 aagaagtact tttatattat aggaaacttc ttgacttgga aaaatgactt tattatcttc
     9781 aaatcttagt ttattatgta acctggcaga atccatcaag gtataatctg gtgcttgccg
     9841 ccacaaacaa ggcccttttt tagacctcaa aaaaagggcc agagcttggg cgtctgtcat
     9901 ctcaatcatt ttataatggt ttctgataga ttgagcttgt tgactagaga tgacataacg
     9961 aaattgatgc aaacgctttt caagctcacc tgcttggctt agtgttttgc caggaaaagc
    10021 ttgatcaact aagctagcca actctttcca aaacatatga tctggcaata gagatggatg
    10081 gaatcgggtg agtagcgttt gagtttcatg caactgacca cttagctcaa atggcatctc
    10141 taccaaatca agaagaaacg ttagtaactg cttagctgat tggttttctt tttcaatagg
    10201 ccatattacc cagaaagttg gagaaccaag tcgattatca atcataggct ctttagctat
    10261 agcacgaaat gcttggtttg ataaacctgc gttttgatag catttcaagg cctgatagcg
    10321 atctaagata gcttgtttca tggccttctc ctcatctttt ttgaccatat tataacacat
    10381 ctaaagctga atcgtcactt gtatctcact ataagcaaaa cgaaacctga agtggttaac
    10441 taagccactg tttgggtatt ttatttaaaa gaacttctcg attaatttta ccattatctt
    10501 ttaaaggcaa acaatcaatc acaagatagt acttgggaag ttgtccagag tttaaataaa
    10561 gagctaaaaa gtctttaata ggtgctaggc aatccttctt agtcaaaact aatactaata
    10621 ttagggtttc gttttctcct tttcctatct ttaaagcgtg cgcttctttg acgtttagcg
    10681 cttggtgagc gagttcaact aggctaggca ggtggcattt cactcctcgt tgattaaccc
    10741 agtcatcttg tcgcccttct aaaattaaac ccgctgggct catttttcct aagtctgaga
    10801 cactgtatgg ttgtgacatt ccttctacac tataaggagt ctctacaaaa atttccttat
    10861 ctttgaaact gatagacaca tcaggaaagg gctgtcctac caaaccatta attttgacag
    10921 cagctctgcc attacaccac gttataaaag ataattcact agccccgtag aaaatgacaa
    10981 tttcaagctg aggaaatttt ttgtaataat gtcttagtaa ttcttgtgat atcatttgcg
    11041 aagaggtcaa taagtgagtc gcagtcatgt tgttcttagt caaataaggt agcaagcgat
    11101 ttaggtaggt cggtaaaagg taaaggtgac tcaccttttt tgcttgccat aagcttaacc
    11161 acgttttcaa agacagcttt tggcttaaaa ccagacatcc cccggcccat agttgagcta
    11221 atgctaagtt aagattgcca gtaaaactaa aactaccatg taagaataga caactattac
    11281 cagtcatacc aaacatctta ttttgataat caaaaagtct cgtccaagtt gatagacaac
    11341 gccaaaataa ttttgctttg ccagtcgttc ccgaactcaa aacagcaaaa tcagcttttt
    11401 caagaagctc accatcgacg tgttttattt gctgctgaaa tgtacctgag atattagggt
    11461 gtaaaatgat aggacagctg ccttctttta atcctcgtaa aaaagagagt aactgattaa
    11521 gatagcgact atgactgatg atatatggta cactgtcttt tattgtctga tcctttatta
    11581 aaaccgcttg ccatagctct tgataagtca agcttatctg atcggctacg atagcttttt
    11641 tatttgggca ttgctttgcc caatattcta gctttgtcag catgactaaa ctccttatga
    11701 tattttagta aaacagcaaa cccttggcca ccagctgctg ctatagcagc tattccatag
    11761 tgtccatttt taatctctaa agccctcatt aagtgcaata ttataatggc tgctgaggca
    11821 ccatagggat gaccataagc taatgcacca ccaaatatat tatagcggtc caataaatct
    11881 gggtaatggg tttcaaatag accatcaata acggcaaagg cttcgttcca ttcaatcgct
    11941 gtcatatctg ccatccctag accatgtttt tccaaaagga cttgactagc cttaatcacc
    12001 attaaagggc tacgctgtgg gtctcctgct acttctacca catcaatcag tttaaaagct
    12061 gaaggctgac tacttagctg taaaaaggca gcagcatcat gggttaggca ggcattggct
    12121 gcactaatca cgtgatcttc tcctaagata gggggtacct ttgacaacac cttacttgac
    12181 aagcgcggtc gaataccttg atcactggct cctggcaggt ctagaataag atcagctaac
    12241 agggcttgct cctggcaata actagccttt tgatgacttc tcaatatcca gtgattcaaa
    12301 tactcctttg taaagccatg ctctcttgcc actcgttctg ccccttcaat catcgcaaaa
    12361 gggctgatac tatctggaga aaactgagct accttgtagg ctccttggcg ccaatcagct
    12421 gaagcatata ccgattcagg ttgtaaggag ctgctttcaa tccctcccac taaaaggttg
    12481 cttgccatac ctgccttgat tttagcataa cccacggaaa gagctgcacc agctgatgca
    12541 cactgcatat caaccgtcaa agcagagaca gattctccta aatgagaata cagggtcatc
    12601 aagcgcccaa tattaccacc cgttccgacg gcattgccac aaatcacttg gtcaatggga
    12661 actggatact tattttgtaa agcattgatg agctttgctc caaggatttc tggttgttct
    12721 ttagcaaatt gtttccctac taaaccgata ggggttctaa ggcctgcagc aatataaaca
    12781 tccgtcatta tattgacctc ctttattttc gatgtcctta aatggttatt atataattat
    12841 ttctgatggt tttctttttt gaactaataa ctgctaaaaa tacacctaaa ttaatttaga
    12901 gaatctcttt agtctcgcta gtgtgccatc aataaaatct ataaaattct ctaacgtcat
    12961 tgaaatgtca agtcccacaa ctattgtcgt agaagtcact tactttgttg ctttaacatt
    13021 caaactgtaa cctttgtttt tgatacaggg tgactttatt atactggaaa accattccta
    13081 agaaaagaaa atcttatata gaataaagaa ttagacacca taaaaaagct aagctgtgac
    13141 acttaacttt tttatggtat tgacaaacct tgtctttact atttattgat agttaatgcc
    13201 tctaactaaa ataaaagcca tgtctgtatc agcctgcaag gcatctgcta agctggtatc
    13261 gttactagat ttgacaggtc tcataaaggc aaaagatctc aaagttccat aaacgttatc
    13321 tagcaattgc atgcaaccac ttgaaatata tctgcttatg ttattttacc gcccttaatt
    13381 tagaccaatc tgctgatgat ggttccaaac aatgcctcta ggaacctgca ctttcataag
    13441 catctaggcc ccatttccaa agcttaagcg acaaggccaa aaccagtaac gacaccacta
    13501 ccaagcctcc aatattaaag agcaggtgct gacctgttaa aaaataagaa gcagggtaat
    13561 aagctgtaaa agcaaaggga atgacaaaac tgattaacca ccgcaaaaag gaatggtaaa
    13621 tggacatagg gtacttggaa aagtcattaa acatgtaaaa aatatagata actgccccag
    13681 actgcttggt ccaaaaagaa atgctggcgg tcgcaatttt taaagaggtg taaatcaagg
    13741 tcgcaaaagg aataaccaaa ataaacaaaa gcaccttagg aagagtccat acgatactac
    13801 cagccgtggt cactaataag ataaccccaa ccaataattc acccagggca tccacttgaa
    13861 atgtctcaac caaaacatga aacaaaggac taattggtct ggtgaggtac ttgtcaaatt
    13921 ctcctttgcg gacaagccgt tgccctaaag cccaaagatt gtcaaaaaag aggtgatcaa
    13981 ttccctttgg aatcaaagaa aagccataaa taaaggcaat ttgttcaaac gtccagcctt
    14041 ctaaagaagg gatatgttgg aaaagtaccg ataaaaagag cagattaaga ccctgagtga
    14101 gaaaaacacc tagcactccc accacaaaat caaccttgta ttccataatt tgtttgaggt
    14161 attgtttgat aaaaatagcg tgcatacgtc tcaattttgc catctcaccc tccttgaatc
    14221 gttaaatggt actgaacctt tttccagatg acttgcgata agactaccat caccagtaac
    14281 caaaagattt gcaaacttag ggcaaccatt atctgactaa ggctgtattt cccaatgaca
    14341 atcataactg gcgtgtaaac caaagaggaa aacggtagaa aactaagcac tatagaaacc
    14401 attttaggaa aaaaagccaa tggaatcaaa ctgcctgaca taaaggcaac caaggcattt
    14461 ttcaataaat tagaccccca aaggttttta aagacaaatg ctgaaaagcc aaaacaaatg
    14521 ttgaaataaa aattgattaa aaaagctaat aaaaggctaa ctaaatacag acaactgctg
    14581 gctaggactt gcaagataga cagccccgcc atgactttaa taccagataa gaccactaaa
    14641 aaaggaaagc cgacactcat tagcacaatc cagcgaaaac caatttccat aaagaggtaa
    14701 gaggctgcaa aatgaactgg tcgaagcagg cgcatgatga tagaaccatc tttcacttct
    14761 tcgccaatca taaaggagct gtctgatttg gtcagtaaag tggtcacaaa actcatgata
    14821 atgtaaaagg tcatgtcaga cagagtaaaa ccattgatta aggattgctt agacgaatca
    14881 aaaacagcct tccagaggta ataagccaca aaagccccca tgacatctcc aatacgatac
    14941 aaaaagaaat taacgcgata ggttattaat tcctgaattc ctgcactgag aaagggtcta
    15001 taacgtttcc aaaatgacca catcttaaag ctcctttcgg tagaaacgac gaacaatatc
    15061 ctcaatatca acatcagtca tcttcaaatc cctgaccgca aaatcagcca tggttttttg
    15121 aataatgtcc gctgtttgat agcgcgaact gtcatattga atgtctaaac taagctcatg
    15181 ccgttccact gtgatgtcag gtaagcccat aaactgtgag accacctgct cctgccctgg
    15241 cctcaactca aaagacagac tcttcatttt tccaaagctt tgctttaatt gggtcaccgt
    15301 tccatcaaag atttcttgcc ctttatcaat catgataatc cgatcacaga gctgttcaat
    15361 atcactcaaa tcatgggtcg tcagtaaaat agttgtctct tcttcttgat taatttgagt
    15421 aatggcacgc cgaatgttgt ccttcacaga aacatcaagt ccaatcgttg gttcatctaa
    15481 aaacaatact ttaggattat gcaacaagga agcagcaata tcagctcgca tccgctgacc
    15541 gagtgataag atccggacag gatccttaat aaactcattc aggtccaaaa cctcatttaa
    15601 aaaatccatg cgcttgcgaa aggctttttc aggcacatcg taaatttctt ttaaaacgac
    15661 ataggtctct tgcaaggcca aatcccacca tagttgtgtt cgttgaccaa aaacagctcc
    15721 aatgtctctc acataatctt gacgattatc ttgtggaatc tttccgttaa tgcgacagta
    15781 acctgatgta ggctttaaaa tacctgttaa catcttaatc gttgttgatt ttcctgcacc
    15841 attggctcca ataaaaccta aaatttgccc tttgggtacc tcaaaagata agtccttaac
    15901 cgcctcaaag atttctcttt ggggatgaac aaaggacttc aaggctccct ttaatcctgg
    15961 ctctttaatg gttttggaaa aatttttttg aaggtgggac acttctatca tgaccattta
    16021 ccattcctcg tttctgttat cgtcttatca ccgttactaa acaatttatt ataacaccat
    16081 gaaaaagact gtgcaaccgt ttgcttaatt ttttaagaaa gaaaaagaag cccataagga
    16141 cctctttttg ccattgatta agcttgtgca tctggacgtg acttgcgagc aatgtctgcc
    16201 aagatgtttt ccacaaattc ttccacagat tcagtgtgag tcgccttact accatagcgg
    16261 cgaacattga ctgatttgtc ttccatttct ttgtccccaa caattaattg gtaaggaatt
    16321 ttgctggttt gagacgcacg aatcttgtac tgcatttttt cattgcggtc atcgacatct
    16381 gcacgaacac cacggtcacg aagggttttt gctacttccc aagcatagtc aatgtgggct
    16441 tcatttgaaa ttggaatcac ggttacttgg tgaggtgcca accaagttgg gaaagcaccc
    16501 ttgtaagttt caataaggat agcagtgaaa cgttccatag ttgagataac accacgatga
    16561 atcatgactg ggcggtgttc ttcgccatct gcaccaatgt atttaaggtc aaaacgttct
    16621 ggcaacaaga agtccaactg aatggttgat agagtttctt cgttaccaag agctgttttc
    16681 acctgaatat caagcttagg cccataaaaa gctgcttcac cttctgcttc aaagtaatct
    16741 acacccatct catcaagtgc agctttaagc atactttgag cattttccca catctcatca
    16801 ttgtcataat atttatgagt atcgttaggg tctcggtatg acaaacgaaa acgataatca
    16861 gtcaagttga agtcagcata aacatcaatg attaattgaa gggcacgttg aaattcttcc
    16921 tggatttgct ctggtgttac gaaaatgtgg ccatcattca aggtcatttc acgtacccgt
    16981 tgaagtcctg atagggcacc tgatttttcg taacggtgca tcatgccaag ctcggcaata
    17041 cgaataggca attcacgata agaacgcaca tgatttttat acacttgaat gtggtgtggg
    17101 cagttcattg gacgaagaac aaactcctca ccatctccca tatccattac agggaacata
    17161 tcttcttgat aatgatccca atgacctgat gtcttgtaca attctacaga agcaagtggt
    17221 ggtgtgtaaa cgtgttggta gcctgatgct aattctttat ctgtgatgta gcgctcaagt
    17281 gtacgacgaa tagtcgctcc atctggtaac caaaatggca agccttgacc aacttcttga
    17341 ctaatcatga acaagtcaag ctctttacca agcttacggt gatcacgttc tttggcttct
    17401 tccagacgag tcaagtaagc tttgaggtct tttttatcaa accaagctgt tccatagata
    17461 cgttgcatca tgttattgtc gctgttacca cgccagtaag cacccgccac attgagcaag
    17521 tggaagactt ggatacggcc tgttgatggc acatgagggc cacggcaaag atccacaaat
    17581 tcaccttgac ggtaaaccgt caagccagca ccagcatgct cattaatcaa ttctaccttg
    17641 taaggatcat ctttgaacaa ttccaaagct tcttctttag tcacttcttc acggatacat
    17701 gggtaatttt ctgtcacgat tttttgcatt tcagcttcga tacgtggcaa atcttcgttt
    17761 gaaatttggc cttcagcatt atctgtatca tagtaaaagc cttctgcaat ggcagggccc
    17821 acacccaaat gaagatttgg gaacaagcgt tttgcagctt gggcaaataa atgggctgca
    17881 gagtggcgaa gcacctcata agcatctttg tggtcaggtg tcacgatttc aatactgcca
    17941 tcttcttcaa tggcacgagt ggtgtcaatg agttgatcat tgaacttacc agctaaagct
    18001 tttttagcca atgatttgct gatggactca gcaatgtcaa acgttgttac gccagattca
    18061 aattcacgca cggcgccatc tggaaaagta atcttaatca tttgctttct cctttaagtc
    18121 agttaagatt tccactaaaa catggatagt aagacaaatt agggacaaga ggttagtgaa
    18181 tagctcaata ccaccacgta gaagaacaat accttattca cgaatctacc ctccctagtc
    18241 cttaaaaaca aggctaatcc cctccaaaac aaaaaaactg accgcatccc aaaggacgca
    18301 atcagcgtgg ttccacctta atttatgcag gacaaaaaac atgccttgca cctctagtgg
    18361 cgataagggg gccacccttt tatgtttgca taaaaaccag gaaggagtat cagattagct
    18421 atccttgtgc tctttccacc aaccagcact cgcttaaaag attctgtcca atctaacttg
    18481 tcttcaagat aattatagca agttttcaaa aatttacaag tcttaatcca gaaaatcttt
    18541 gatagcatgg accagcttga taggagcctt gactactttt actgaggtga tagctgtgac
    18601 cttagcagca cgtttaggca agtgaatagc accttgcact aattttagag aactactttt
    18661 accagaatga ttaagataaa gactcagctt ttgagcttct ttgctatttc tagcaattaa
    18721 cgtatctaaa tagaacgtgt aaatagattt tccaaagtgc tgtgctgaga tttcataacg
    18781 tttttttgct aataaccgtt tatccataac tggtgttttt agtatggcat caataacagc
    18841 atcagttaaa tctgtttcag cgtaataaag agtgccaaac attttatcag tcaccacatc
    18901 atctaaataa ggattgccat gagcaataat aggagtgcca ctagccaaac tttcaatata
    18961 ggtcaagccc tgagtctcac tagttgatgc tgagataaag aaatcacaag ccttatagta
    19021 cagagcaacc ttatcatgag gcaccatgcc tgtaaaggtc acgtgtttgt caacctctaa
    19081 ctgcatcgcc aagtgtttca aatcttgcaa atagggacca tttcctacaa taataagctt
    19141 tatcttggca ttttcagcca aaatagctgg catctgattg atgatagctt gaatattttt
    19201 ttcataagaa atccgtgaca aactcaataa catggtttca tcaccagcaa tgcccaattc
    19261 tgcttttaag ttggttactt cttctgctgt gatgtcatca cgaatatatt tttccaaagg
    19321 aatgcctgtt gggataaccc gcttagggat agtcacttcg taaccttcta gaagattgag
    19381 gacaatccta cttgggcaga taaccccatc caaatccttc aaatagcccc taagaagagg
    19441 tttgaccata cttggtcgaa tgatttttcc gttggcaata taactcacgt agtcctcata
    19501 ttgggtatgg taagtatgga caacaggaat tcgcaaagct ttgcctatca ttttccctaa
    19561 taagcctaag ctaaactcag tttgcgtatg aataatatca agattatagt gttttgcaat
    19621 tttgtatgac gaaatgaggc cacgataaac cacacgtcta tccgtaaatg acacaaaagg
    19681 aacacttggc agtcgaataa tggtcgggtc ttcaaagcgt ttgacatctc tatcagtagt
    19741 ggtgaaaata taaacttcgt gaccttcttt ttctagctct tcttttaacg tacgaatact
    19801 agtagcgact cctgaaactt gtggaaaata ggtatctgtg aatagaccta tacgcatctt
    19861 ataactccat tactttttta tagacatcta ctaaggcatg gccaaccgtt tctaggcgac
    19921 gactctgggc aaccttgtaa ccagcttcaa ctttgttgct ggcaccagaa aagacttttt
    19981 tcagagcttc tataaaacct ggtatatcgg ttgctaattc cgcactactt tgatcaaccc
    20041 atccgtagta aactggtata tcacgtaaaa caaggtgctg gcgactggcc aaggcttcta
    20101 agacaacaat gccttctgtt tcttcacgac ttggaaagaa aaaggcatct gcaccagtca
    20161 tggcaccttc ataaacatcc cctttaatgt atcctgggaa aataagattt ttcgggtggt
    20221 taccattgac catttggcga acttgagcag gaatgaccca tttgttggtc tcgccaaacc
    20281 agataaaacg aacatctggc atagcttggg caactttgac aaagtcatca attcctttcc
    20341 tcagaaaaaa taatcctgct cccataacca ctttttcacc ctctttaatg tcaaagtagc
    20401 gacgaaaagc tgcttccttt ttagaatctg ctccgtactg ctccaaatca atcccatttg
    20461 acactgcaaa aataggtgac ttcactccat aactctcaat caaagactta gaatataggg
    20521 taggggtaat gatagcatct gccttattgt aaaagtgaca aaggtatttt ttaaaccaag
    20581 gagataatag atttgaaaaa ataaaagaat ttctaaaatc ttcttctgta gaatgcccat
    20641 gcatgataac cttcttacca gctttttgtg cttttatcat cagcagccaa ctttttaaac
    20701 cataggtatt gagatgaacc aaatcataag tttctcttgg attagtcgta aaatgttgac
    20761 caacaagtga caaggcttta gcctgatgct taatcgctcg accaattcct gattttctta
    20821 gataattttc tgcttctaaa tacaataaga ctttcataag ttctattata gctcatttta
    20881 tagtattttt cactaagaca tgcaaaaatc agccctaagg ctgattttag tattgaagaa
    20941 acagtctatt gtgatacttt ttgttagatg gtgctcataa gtcacagtta cttagttgtc
    21001 ccacgttttt taataccatg attcaaaaga atttcttttt cttccaactc ttctttgttc
    21061 atgattttag tcaacatccg catgctaaca gcacctaagt cataaacagg ttgactgata
    21121 gaactcaaat tgggacgagt gtattgaacc actggtgaat cgttgctggt gatgatttca
    21181 aagtcctctg gtacgcgttt acctgcttca aacaagccgt ttaaaaggcc tgctgccaat
    21241 tcatcttcag ccacataagc cgctgttgct cctgagttaa tgacgcgctg cgccaattca
    21301 aatccttctt tataagaata gttcgcttca aaaacaagac cttctttgaa gtcaagctta
    21361 ttgtgcttca acccttcttt gtaccctgct aagcgcactt taccattgat atcatcaatg
    21421 agtggtcctg acacaaaagc gatacactta tgattttcag ctaaaatatc aacaacgttt
    21481 gacaccgcag ctctatagtc aatgttgacg cttggtaatt gatgatcaag atctacagtt
    21541 cctgccaaaa caactggagt acgagagcgt gaaaattcgg cccggatttt ttctgtcaag
    21601 tgatgcccca taaaaataat accatccact tgcttggcaa aaagggtatt gacaacatta
    21661 acctccttgt catcatcttc atcactggag gcaagcacaa tattatattt gtacatagcg
    21721 gcgatatcgt caataccctt agctaagata gaaaaataac tatttgcaat atttggaatc
    21781 acaacgccaa cggttgttgt ctttttgctg gcgagaccac gcgcgacagc attgggacga
    21841 tagtcaaggc gatcaataac ttctaaaact ttcttacgtg tattttcctt aacattttta
    21901 ttgccattaa caacacgact aacggttgcc attgaaacac cagcttcacg ggcaacatca
    21961 taaattgtaa tggtatcatc tgtattcatt ttttatcttc ctttctattt gaaaatgacg
    22021 ctttcaaaaa gtattttagc atgatttgta accactttca agtgttatag gttaattttt
    22081 gaaaaagttt tcaaaaatta actcgaagct tgatttttta agcttttttt gaaaaaatag
    22141 taagtagaaa gaaggttagc ctatgacaaa attagaccaa atacgtttgt atcttgacca
    22201 aaagggagct gaattggcga ttttctcaga tcctgttacg attaactatt taactggatt
    22261 cttctgtgac ccacatgaaa gacaactgtt cttgtttgtc taccatgatt tagcccctgt
    22321 gctttttgta cctgcccttg aggtggccag agctagccag gccatatcat ttcctgtgtt
    22381 tcgttatgtt gactctgaaa atccttggga aaagatcaag gccgttttac caaatacagc
    22441 agccaaaacc atttatgctg aatttgacca ccttaacgtc aataagttcc atggcttgca
    22501 gactatcttt tcaggacaat ttaacaatct aacctcttat gtccaaggca tgcgcctcgt
    22561 caaatcagct gatgaaatca ataaaatgat gattgccggg cagttcgctg ataaggctgt
    22621 tcaagttggt tttgacaata tttccttaga tgctaccgaa acagacgtca tcgctcaaat
    22681 tgagtttgaa atgaaaaaac aaggcattca taaaatgagc tttgacacta tggtcttaac
    22741 tggaaataat gcagccaatc cccatggcat tcctggaaca aataacattg aaaacaatgc
    22801 cctacttctc tttgacttag gtgttgagac cttaggctac accagtgata tgaccagaac
    22861 tgtggctgtt ggtcagccag accaatttaa aatcgatatc tacaatctct gcctagaggc
    22921 acaactagcg gctattgact ttatcaaacc aggtgtaaca gctgctcaag tagatgctgc
    22981 tgctcgtcaa gtgattgaaa aggctggata tggtgagtac ttcaaccacc gcttgggaca
    23041 tggtatcggc atggatgtcc acgaatttcc ctccatcatg gctggcaatg acttggtcct
    23101 tgaagaaggt atgtgcttct ctgtggaacc aggcatttac attccaggta aagttggtgt
    23161 gcgcatcgaa gactgtggcc atgtcactaa aaatggcttt gaagtcttta cccatactcc
    23221 aaaagagttg ctttattttg aaggctaagc ccctaccatc tggttaaaca aaccctcgcg
    23281 aaaggaaggt attcgtatgg atcaaaccat tcatcaccaa atacagcaag cactacactt
    23341 taggacagcc gttcgtgttt ataaggaaga aaagatttct gatgaggatt tagcccttat
    23401 ccttgatgct gcttggttaa gcccttcttc tgttggctta gaaggctggc gctttgtcgt
    23461 tttagacaac aagcctatta aagaagaaat caagcccttt gcctgggggg cccagtatca
    23521 actggaaaca gctagtcact ttattctttt aatagccgaa aaacatgcaa gatacgatag
    23581 ccctgctatc aaaaatagcc ttttacgacg tggtatcaaa gaaggtgatg gtctcaacag
    23641 ccgcctaaaa ctctatgaat ctttccaaaa agaggacatg gatatggcag ataatcctcg
    23701 ggcactcttt gattggacag ctaaacaaac ctatatcgct cttggtaata tgatgatgac
    23761 agctgctctt ttgggcattg atacttgccc tattgaaggc tttcattatg ataaggtcaa
    23821 tcatatccta gctaagcata atgtgattga tttagaaaaa gaaggcattg ctagcatgtt
    23881 atccctaggc tatcgtttgc gagatcccaa acatgctcaa gttcgtaagc ctaaagaaga
    23941 agtgatttca gtcgttaaat aaggagatta aaatgaaagc attacatacc tgtatccgcg
    24001 tcaaagacct tgaccagtcg gtagcttttt acacaagcgc cttccctttt aaggaaaact
    24061 atcgtaaaga ttttccagat agtcaattta ctcttgttta tctggcttta gaaggagaaa
    24121 gttatgaatt agaattaacc tacaattatg gtcatggcga ctacgactta ggaaatggct
    24181 atggtcatat tgcccttggc tctgaacatt ttgaagcaga ccataaaaaa catcgacaag
    24241 caggttttcc agttaccgat atcaaagaac tggctgacaa atctgcccgc tattatttca
    24301 ttcaggatcc agatggctac aaaattgaag tcattgattt aaacaactaa tactattaac
    24361 aacagaagct ttaaagcttc tgttgtttta ttttttaacc tgacgattgg gataatctca
    24421 ttattaacag agttttgaaa tatctcagct tttgtgttac aattgcccta aaattccatt
    24481 tgaaagaaaa gtctatgaca ctactatcta tcattgtccc ctgctttaat gaagaagcaa
    24541 acattctccc ttattttgaa gaaatgcatc aactagaaac gtccatgtcc aaccaacttg
    24601 cttttgaata catttttatt gatgatggtt ctaaggataa caccttaggt attttgcgtg
    24661 agctagctgc tcgctttcca aacgtgcatt atctgtcttt ttcacgccat tttggcaagg
    24721 aagctggact cttagctgga ctaaaagaag ccaaaggcaa ttacattact gtgatggatg
    24781 tggatctgca agatccaccc gaacttttgc cagttatgta cgctaagcta aaagaaggtt
    24841 atgacatcgt tggcaccagg cgccaaaata gacaaggaga accactaatt cgttcaatgt
    24901 gttcgaatct attttatgga ctgatcaaac acttatcaga tactgaaatg gtcaatggag
    24961 ttcgggatta tcggttaatg accagacaag tcgttgatag cattcttgag ttgggagagg
    25021 ttaatcgttt ttcaaaaggg atcttttcgt gggttggtta tcgtatcacc tatcttagtt
    25081 ttgaaaatca aaaacgcaaa tatggaaaaa gtcgttggca tttttgggag ttgctccgat
    25141 attctttaga tggctttatc aacttttcag aaatgccgtt aaccattgct acctggacag
    25201 ggactttcag ctttttgatt tctattttcg ccatcttatt tatcattatc agaaagatac
    25261 tctttggtga tcctgtctct ggttgggcca gcactgtctc tattatttta ttcatgggag
    25321 gcattcagct cttttgcatg ggtattattg gaaaatatat ttcaaaaata ttccttgaaa
    25381 ctaaaaaaag gcctctctat attatcaaag aaaaacatta aattaaaaca tttttctcat
    25441 caatcgtttc ctactccttc tgtgcgcgaa gctttctttt taagcccgtt ataccaaatg
    25501 ataatgacct ttccaaaata gtatcacctt ccttaacact ttaagtagaa aaggaactat
    25561 taggttgagc ctttttgcaa atcctagcga ctaatgttat aatcgtcata ctattgaaaa
    25621 aggagttctt tatggagtgg attaaattaa tcggtattgt gattatcgtg ttagggttta
    25681 ttttaaaatg tgatgctatc gctaccgtag ttgttgcagg acttgttacc gcgctggttt
    25741 caggaatttc gtttattgat tttctagata ttttaggaaa agaatttacc aatcaacgtt
    25801 tgctaaccat tttcttcatc actttaccgc ttatcggctt gtcagaaact tatggactga
    25861 aacaccgagc tactcagttg attcaacgtg ttcaagccct taccgtcggc cgtttattga
    25921 ctctctacct tattattcga gaattagcag gtctcttttc gattcgacta gggggacatc
    25981 cacagttcgt tcgccccttg attcaaccta tgggagaagc tgctgctaaa gccaacattg
    26041 gagaagagtt gaccgatgca gaaaaagatg atatcaaagc tatggcagct gccaatgaaa
    26101 actttggcaa tttctttgcc caaaatacct ttgttggcgc aggaggtgtc ctacttatcg
    26161 ctggaacttt agaacagctt ggctatgatg gtaaccaagc taaaattgca ttttcttcta
    26221 ttttaattgc tattatctct attattatag ttgctattta caattacctc tttgagaaaa
    26281 aaatggaacg tcaacatcag aaaggagata actaatggct aacattgcaa accttgtttt
    26341 agaaacgata tatatcctta tcggcttgca actgtttcac acagcctact gcacctttaa
    26401 agataaaacc aaccctgtct attttgggac agctctgttc tggggattgc tgggtgttac
    26461 cttcgttggc ggtgcctttt tgcctaataa agttatcggt tttatcgtga ttgtcctagc
    26521 tcttttaacc ttgtttaagc aagttcgtat tggaacatta ccagccttta atgaacaaaa
    26581 agctgaagaa agtgctcacc gcattggtaa ctggattttc ttaccagtta tgttaatggc
    26641 tatgatttct ctacttcttg ccttgatcct tcctgatttc agtaaaagtg ccattgggat
    26701 tgctggtatc ttagccacta ttgctatttt aataatcact aagcaaaaac catctgctct
    26761 tttagctgaa aataatcgga tgaaccaaca agtgtccaca agcggtattt tgccacagct
    26821 cttaggagct ttaggagcta tttttgcagc tgctggtgtt ggtgatgtga ttgcttctct
    26881 cattcgggag attgtacctg ctgatagccg cttctttggc gttctcgctt atgtgcttgg
    26941 catggttatt tttactatga ttatgggaaa cgcttttgct gcctttaccg ttattacaac
    27001 cggtatcggt gttccttttg tttttgcttt aggagctgac cctattattg ccggtgcttt
    27061 agctatgact gctggttttt gtggtacctt attaacacca atggcagcaa actttaatgc
    27121 cttgcctgtt gctttgatgg agatcaaaga tcgaaatgca gttatcaaaa agcaagctcc
    27181 tatcgccctt gttttaatta ttagtcacat tgccttaatg tatttacttg cttattatta
    27241 acatttttgc taagttagta gcgtcagtta ttcattgaaa ggacattatt atgaaaattc
    27301 ttgtaacagg ctttgatccc tttggcggcg aagctattaa tcctgccctt gaagctatca
    27361 agaaattgcc agcaaccatt catggagcag aaatcaaata tattgaagtt ccaacggttt
    27421 ttcaaaaatc tgccgatgtg ctccagcagc atatcgaaag ctttcaacct gatgcagtcc
    27481 tttgtattgg gcaagctggt ggccggactg gactaacgcc agaacgcgtt gccattaatc
    27541 aagacgatgc tcgcattcct gataacgaag ggaatcagcc tattgataca cctattcgtg
    27601 cagatggtaa agcagcttat ttttcaacct tgccaatcaa agcgatggtt gctgccattc
    27661 atcaggctgg gcttcctgct tctgtttcta atacagctgg tacctttgtt tgcaatcatt
    27721 tgatgtatca agccctttac ttagtggata aatattgtcc aaatgccaaa gctgggttta
    27781 tgcatattcc ctttatgatg gaacaggttg ttgataaacc taatacagct gccatgaacc
    27841 tcgatgatat tacaagagga attgaggctg ctatttttgc cattgtcgat ttcaaagatc
    27901 gttccgattt aaaacgtgta gggggcgcta ctcactgact gtgacgctac taaacctatt
    27961 aaaaaaacag agatatgaac taactctgtt ttttttgtgc taaaaatgaa agacctaggg
    28021 aaacttttca taggtctttc tcaattgtca tcttaatcta atactacttc taacatcagc
    28081 gggtatagtt taccagtaat taagaaacgt tgttgatcta aatgagcaat cccattcaaa
    28141 acattaaggt cagggtaatg ggacttatca agatttaagg cttttaacaa aggactaata
    28201 tcataggtgg ctaccacctt tccagaatca ggttggagtt tgacaatagt attggtttgc
    28261 caaatattgg catagagata accatctaca tactctaatt cgttaagcat tgagatgggg
    28321 acactttcta tagcaactag tacggtatcc aataaagcaa aatctttggg gtctcgcttt
    28381 tgcaaaaaag catttccact agtcatccaa agacactggt tttccttatc ataggctaat
    28441 ccccagccat ctccttcaaa tggataggcc cctaaacaat tgcaggtagc cttatcaaac
    28501 ttgtaggcta caccctcttt ataggttagt aaccaaaaat aatcctcaac aactgttagc
    28561 ccttctgcaa aaactgtatc tggaaaagct attttttctg aaaagatctc ttgagtaagg
    28621 tcatacaccc ctacttttga gaaaccataa cgaccggcac taagtaatat atgattgttg
    28681 ttgagttgtt ctaatccctg cgtgtataag ttgctatcat aagaataagt tcgtaacaaa
    28741 caaacctgtc ctttagttat cataatactc attcacctta ccttgcattg tagtgtttca
    28801 tttgtttttt aatatctctt tcctgatcac ggcgtttaat ggtttcgcgt ttgtcatact
    28861 cgtgtttccc tttagctaga ccaatcagaa ctttagcaaa gccatccttg agataaactt
    28921 tgagaggagc caaagtcatg ccagatcctt taagttcatt tgctagatgc gtaatctcac
    28981 gttttttgag caacagtttg cgggtccgtt caggatcagc gttccaaata ttaccttgtt
    29041 caaaaggtgc aatatgcacg ttgaccaacc acgcttcccc attcttaatt tgggcaaagc
    29101 catctttcag ttgaatacga gctgcacgaa cacttttaat ctcagttccc gttaagacaa
    29161 tccctgcttc aacggtttca acaatgtgat aatcatgtct agccttttta ttttgcgcca
    29221 gtatatgacc ttcacctttt gccatagtta actcctttta cgactcttct tttttgcttg
    29281 ttctttgtaa aatggttttt tagacccttt tttagtttta cccttggttt ttgccttggc
    29341 tacttcttta ggttcttttt tctttatccc tttagatgat tttgaggatt tacggcgatc
    29401 tcgtcgacta gctttatccg acatcttgat tttttcaacc acatcaaagt cgctaggtaa
    29461 atactcaaag tcaatgtcac cagtttcctt gtcagcctta accaatttca cacgaatagg
    29521 ttgaccaact ttaaagactt tacctgattt ttcaccttga aggcttagcg ttctttcatt
    29581 aaagtgatag tattccggta agctagtaat atgaaccaaa ccttcaatcg tatttggcaa
    29641 ttctacaaaa aaaccaaatt taacgacact ggccacgata ccgtcaaatt cttcacccac
    29701 atactctgcc atatactcag cttttttcat ggcttcaacc acgcgttcag catcaatagc
    29761 acgacgctct aactgtgaag aagatgttgc taattctgga atgatctgag taaaatggtc
    29821 gcgcttttct tgactaggtt gactgtactc acgaaccatg cggtgaacca acaagtcagg
    29881 gtaacgacga atcggactgg taaagtgagt gtaatattct gcggccaaac cgtaatgccc
    29941 atgattatgt tcagaatatc ttgcctgttg catagagcgt aagagcatca tgttaagcac
    30001 ttctgctcct ggttgacctt cgacttttgc cataaaggct tgcaaggctt cctgagaaat
    30061 cttgttggct gttccttgaa tgtgaatacc aaaggtgcta gcgtaatcaa taaattgttg
    30121 aagtttttcg gcctttggtt cttcgtgaat acggtagata aacggtaact tagcttttgc
    30181 aaaatgttct gccacacact cattggctgc tagcataaag gactcaatca tccgctctgc
    30241 aattcccctt tgacgaagca ccacatcaac cggtattcct ttgtcattaa caataacttt
    30301 agcttcttgc gtatcaaaat tcaaggcgcc tcgttttgac cgcatagctt ccaaaatcct
    30361 atgcaaagcc accatgaggg tcacatcatc agcaatgcta gcgaattctt ggagcgcttc
    30421 ttcatctcct gcaatcatgt cattgaccgt actatacgtc attcggtaag ttgtcttaat
    30481 cactgattga cagatttgat aattgaccac atgcccttga gaattaattt ccatcaaggc
    30541 tgactgggtc aaacgatcaa cgttagggtt caaagagcag atgccatttg acaaacgctc
    30601 tggtaacatc ggaaccacac gatcggtgac atagacagaa gtgccacggg cgatagcttc
    30661 cttatcaagg gcagatcctt ctgtcacata atatgagaca tccgcaatat gtaccccaag
    30721 ttcatagtta ccgttatcca atagcttaat atggatagca tcatctaagt ctttggcgtc
    30781 cgcaccatca atagtaatgg tggtttcttg acgcaaatcc acacggccta ttaaatcttt
    30841 agcagttggt gcttctgaaa tagcattggc ctcggctaaa acttctgctg ggaactctga
    30901 cacgatatcc atcgattcaa ggacttctag gacatcaatt ccaacgtctc cttgatggcc
    30961 tacgatatca cgcacactgg ctacaaaata atcgtgcccg cgtgtcgggt atttatcaat
    31021 atcaaccttg atgatttcag tgcctttaag cacaacaggt tcttttttga tatagatttt
    31081 ttgttgaatc ttttgatttt tggacctgat ataaccagca tattttggtt tgtcatcatc
    31141 taaaataaag gttccaacag ctgtttttaa agaacggtca acaatagcaa cgaccttagc
    31201 ttcagccgct gtgcctttga gacggtctgc tggcttttta accactactt cgacagtatc
    31261 accatcaata gcatacccaa catcattacg accgataaac atgtcatctt cattctcatc
    31321 cacatgtaag aaaccaaagc ctgctttatt ggcacgaaaa acaccctgaa cagtaggttc
    31381 ttttttcttt tcacgctctt ttctaagagc cagactgcca tcatctgaaa accgtaacag
    31441 gctctgactt tccattttgg aaatggtttt aataaggctg ggaaaatgct tagctccagc
    31501 catctccaag gccgcagcca agtcgttcac attggacttg ccatgctcct tgaggtaagt
    31561 cattattctt tctttcatca tacctaattt ctatttctgt tttgtttgtt ggaggatatc
    31621 aatccgtttc gctgataaaa aaatgagcta gactaactct gtcaagctca ctccttattt
    31681 acttgataaa atggcaagca caagtgcaat cgctagccaa aaaaatacta gaactgctgt
    31741 gaatcgttgc atgaatgctt caaagccacg agcttttgta cgttcaaaca aggcttctga
    31801 accgctgcta tcaaaaacat tgctacttgg gtttttttga ggttgcataa aaatagcaat
    31861 cacaagcaat accgacagta ctagcaatat cgtaagtagt aagttgtaca tgttatttcc
    31921 tccactattg tcatgaccat tctaacacaa ttcttatcaa gtttcaacat gcagggtcac
    31981 ttttctctcc ttggggcagt atttgggaac ttttattttt tccggatgac tgcttttatt
    32041 tttactggtt agataattgt tactgccaca ctcactgcat tctaaattaa tttttactct
    32101 cacacaatat ccttaacttt aatatatttt cgaaatataa tcaaactcca tattaaatta
    32161 acaaacacaa tcaaactagt cacataaaaa acagaacggt agcctagcac tacagccaca
    32221 tttgatccga taaaagggcc aatgacttga ccaaggttac taaacatttg attataggca
    32281 aatactctag aaataccttc ttttggtgtt aacttagtta gtaaagagtt aatacttggc
    32341 atcaaagcgc ccacaccaaa cccataagca aagcgtagta ctcctaattg aaaactggtc
    32401 tgcgctaagg cactgctaaa atacatgata aagctataac acaaagcggc taacaataac
    32461 cgatgattgc caaatcgatc tcctaatttt cctaagtaag aactgctaaa taagctagaa
    32521 aaccccatgg ccgaaactac caggcctgaa gtaaacatta agttgtgagt ttgaccaaga
    32581 tgacgaatat acaatgataa aatgggggtc actgattgag ctgaaatttg aataatcata
    32641 ctagtcacaa acaaaccaag cataatctgg ggacttttta cctgttttaa aatgactttt
    32701 gtaggtatca tctcaaagcg cctaatcggc ttaaactctt ctttaacata aacagctgtc
    32761 attaaagaac ataaaaataa tatcacacca actaataaga aaacttgtcg aattcctaat
    32821 agctctgcca aaattccccc tagtaaagga ccaattaaag cgcctgctgt cactcctgtt
    32881 gctaatgtgc ctaaagcata acctgactcc tcttttgggg cttggctggc aatcaaggca
    32941 gtggcatttg gcacatagcc cgcaaagaca cctgttaata atctcaaaat gagtaaccaa
    33001 aacacattag gaataatagc taaaccaccc attgtaaatg tcatgacaaa acttgctcgc
    33061 agcatcatag gttttcggcc ataacgatca gccaacttcc cccaaacagg agcaaataaa
    33121 gctgaagcaa gtgccgtcac agcaaccgcc agaccagcat agtattctac taattctgtc
    33181 ggagtaccta aattttccac atacagagcc ataaaaggca taacaagtga aaagcttgcc
    33241 ccagtaaaga agttaccaag ccaagccact tttagatttt gccgccagtt cactgtcgtt
    33301 accgctttat cactaataga tcatcatctt ctttccatcg ggcctttgat tattataaca
    33361 catttctgcc aaaaacagtg tcccataata gtgctaagtc tcatctgata gccttcatcg
    33421 tttacgtaag ctacccagta aacctctgac cagttctcgc ccaattgttc taaccgtcgt
    33481 actaataaac gcatctgttg ctttttcgac gactgttttg cggggacgcc caacagaacg
    33541 gcgattagct tttttggtag cagcttgtgc cttgatggct tctttagctt ctttttcgcg
    33601 ttgtgctgtc gctatcgctt cttgggccag tcggttgtct tctaaaacct tagcagctaa
    33661 tttttcataa gcagaatcac gatcaatgga ctgactatat ttactggcaa aaggtgagga
    33721 ctgcacaagc tgctgacttt ctatatctga caaaacagca aagctactct taggcggcat
    33781 gatataggca cgttcaacga tggatggttg tcccttatca ttcaaaacag aaatcaaagc
    33841 ttcgccgact tctagttctg taatcaccct tgccacatct aagtcaggat tttgtcggaa
    33901 agtatccgca gcgacacgaa ccgccttttg ttctttcggt gtataggcac gtaaggcatg
    33961 ctgaatacga tttccaagct gagccaaaac cgtttctggc aaatcaagcg ggttttgagt
    34021 cacaaagaaa atcccaactc cctttgaacg aataagtcgc acaatctgtt caactttttc
    34081 caagaaaact ttaggggcat ctttgaagag caagtgagct tcatcaaaaa agaaaaccat
    34141 cttaggtttg tccaaatccc caacttctgg caataattcg tacaactcag acaagagcca
    34201 aagtaagaag gttgtgtaaa gggtaggaga ttgaaaaagc ttagtcgctg acaaaatgtt
    34261 aatggctcca taacccgaag caacatctaa ttgcatcaaa tcagccacat ctaaagcagg
    34321 ctctccaaaa aattggtggg ccccttcttg ttctaatgtc aagagacttc tttggatagc
    34381 cccgatagat tgcttggcga tattaccgta atggctggag tagtctgagg catggtcacc
    34441 aacttctttt aagatagcct gcaagtcttt caaatcaatc aaaagccaac ctttctcgtc
    34501 ggcgatttta aagacgatgt ttaagacacc tgtctgggta tcattaagat tcaataaacg
    34561 cgacaacatc atcggtccca tttcagaaat tgttgtccgt aagggttgac cgttttgacc
    34621 aaaaacatcc cacattctta caggaaaggc ctgaggctgg taatcactta ccccgattgt
    34681 ggctaaacgc gcagccaatt tatcagtcac ctctcctgct ttagtaagat tagacaggtc
    34741 tcctttgata tctgccaaaa agactggcac accagctaaa cttaattgct ctgctagcac
    34801 ttttaaggtc acagttttac ctgttccagt cgctccagca atcaaaccgt gacgatttag
    34861 catccctaat tgtaaggcta tcggttgact gccttttgca attgtcattg tagtcatagg
    34921 cttcctcttt tttgatgatc atacttgtcg atatgtgttt acccataagt taaggtgttt
    34981 agaagtgtct cgctcttaat actattataa catataaccc ttgatgcgct aatttaaaag
    35041 ggaccagcta gttttagaca gaaatagctt gattttgaca gctatgatac gaatctctca
    35101 gcttagctac ctcggattgg ctaatgacaa caacgcagat tgtacttgct ttattaacgt
    35161 ttccatgtct ccattattat caatcactag actggcatag ggcttttttt cctctattgg
    35221 taactgactg gctattcgct gtcttgcttt tcctttgtct aaacgatttc tagccattaa
    35281 gcgctgcaat tgtgtctgag catctacata aaccagccaa atcgcatcaa accagtcttg
    35341 gtagcccaat tccattaaaa ggggaatatc catgaaaaaa atggcttggc tctgtgctaa
    35401 gtggtcacgc ttagccgcca attcttcctt aatgatttga ttttgaatgg ctgaggatgt
    35461 tgccatatta tttgggtttg aaaaaagcat ttctgacagc tttgtgcgat ctaactcacc
    35521 atctgctttt aaaatctgat tcccaaaagc ttctctaaga gcctcgtaaa gacgaccgcc
    35581 tttttcttgc aaatcatgga ccacttgatc cgcatcaatc acctgataac cagcttttct
    35641 aatgacctta accacagtcg attttcctga tgcaatgcct cctgtgatgc caataatcat
    35701 ggtctttttt tctggcattt tggacaaatg tgcgtccccc ttcctccaac ttttaatttg
    35761 acaatagcct gtccacaacg aggacacggt ttcccagttt gaccataaac ctgcaaatag
    35821 tcctgcatgg tgccatcagc tcccaaagca ttacggtaag tacggacggt cgaaccgccc
    35881 ttttcaattc ctaaagctag gattcgtatg gtttcatcat gcagccgttt tatctccgcc
    35941 ttattcaacc tagaagacgc agtttctgga tgaaccttag cagcccaaag tacctcgtct
    36001 acataaatat tccctaaacc agcaaccaag gtctggtcta acagatgtgg ttttatgggt
    36061 ttttgggagg atagcagagc agcctcaaag gtttttaact taaaggtctc tttctttggt
    36121 tcagggccta gttttcgttt ggcaaaaaat gctgataatt gagattttgc tatcaaatca
    36181 aaggttccaa atttacgcac atcttggtaa accaaggtcg aaccattttt caattcaaaa
    36241 aagacatgaa agtgtttgtt atcagggacc tcatctggaa aaagcaaata tttgccttcc
    36301 atccgcaaat gtgataccaa cactaattgc cccaaatcaa ttaataaata ttttccccga
    36361 cggccaactg actgaatgat ttgaccaggt agggtcagag caaacgtctc taagtcggtt
    36421 ttaaccattt ttggtacttt aagcgtaaca gccacaattt cttggccaag cactaaagtc
    36481 tcaagccctc gtcggaccgt ttcaacttct ggtaattctg gcataatccg ttatttaaag
    36541 gacagctaga ccctttgctg ctacaaagaa taaactcagt ataaaaaagt cactcaatag
    36601 actcaggtct aggctactga ctgcgtttca aaaaacacag ctgtccttct cctttcttat
    36661 ttcttatttt acgaacacat ctgatagaaa gcaactcata aatctgcctc agaccagtcc
    36721 tcatccccct tacctcccag cactatttga cggtaatgtt ccttataact ataatacaat
    36781 gagtcctctc caagatactt aaaaatctgc aaggcctgcc gcatgtcctc ttctccagac
    36841 atttcttcct gctttaattt ataataccca tgcacatata agaaaacagt cttttcataa
    36901 aaattgagct cgtctcgcag caaaagatca attttgttaa tcaaatagcg actatgttca
    36961 aagcaagaat ggtcaatact gataatgaga caattaatgg ataactgagt cactaacatt
    37021 ttattggttt tattttgttc cgaataagca aaagatgcta ccatttcctt agtcaataaa
    37081 aatagggtat tgtaattcat gaagcgcgag caattcccca gaagaatgat ttcataatat
    37141 ccccactgtt ccactttaaa taaataatct gtgaggcgag tcaattcttc ttgactaggt
    37201 gcaattgagg agtcaataga aaaaagaata gctttaatca tcgttcgttc ataatcttta
    37261 tgggcataat ccttcaacag tttggtcaat ttaaccacgt ttttctcagc ataacatttt
    37321 ctcgcctgac ttaagagcgt gaaaaaatgc gtatgcgtct tggaatgagc cgaaacaaat
    37381 tcgtcaatcg taatattcag tttatccaaa agattaagca agcgactgca ggtgatttct
    37441 gattcccctc gttcaaatct tgaaatttgt gacttggaca ggtattcatc tgttaaaaaa
    37501 gaaatgctaa cctgcttacc cttacgtaat cgccttaagg tttttccgag ttctttttcc
    37561 atttttccca ctttcacaac aaaaaattaa aatctgctaa atccattata taatacaagt
    37621 acaaaataga aaatcagaaa ggagacagtc atgaaaaaag ttaataaagc tttgcttttt
    37681 acattgatca tggatatcct gattatcgtt ggtggataac ctttcgctta aaaacaatcc
    37741 acttttggga aacccttata tttagtttgt aacaggaaaa ctaatagata ttaaaaagag
    37801 tgttagcgac ctagcggcag ctttactctt aagtgcgttt gctgccattg acagcaaggt
    37861 caaagctgat gaaacagcag gggcataccg ttatgatgtg tctaaacaaa acacttacta
    37921 tcaaactatc agctccatta accagcaacg acctggtgcc actctcgacg aatgaggtga
    37981 ttattatgga acccttactt catttacttc ccaaagtcgt accagtactt atacacttgg
    38041 tagacaaagc caacgtatca tggttaacag tgatattaca gttacctata ctcttaaaga
    38101 caatttggta gcccttcata aggaatataa aacctttgtc aaaaaaatat tacagataaa
    38161 ccgatcacca gtgactacga caaggctaag gttatttata accatttaat caacgaatac
    38221 acttacgcca caaaataact tagcaccaat cgcaaaaccg taagcagcat caacaatcat
    38281 actcctaaag ctctttatcg tggcaagtgt ggtgcttgtc aagcttatgc cgtcatgttt
    38341 aaagatatgg cagaagctgc aggcttatca acttggtatg tgataaaaaa ggattgaatg
    38401 agcaccatac ttgaaacatc gttaccattc atggtgttaa ttcctacgtt gatgctacct
    38461 ggaatgattc agcatacacc acaaggtatt tccttgctgg aaaagacgtg aggaatactg
    38521 agtaccgttt agatagccag tacaaccact tggctaatac tattttaaat accagctacc
    38581 aaaaaggcta aactaacaac aactacattt ttcaactaga aaggcctaaa caatgaccaa
    38641 aaaacagttt tatcttatct tagctattag catactgtgt tttattcttg ccgtcagccc
    38701 taaaccacca attcctaaac acactattga aaggaaaagg actacgatga ccataggcca
    38761 ctcaaaacaa aaccctcaca aaaaagaagc ccttaaacac aaaaaaaata aaaataaggc
    38821 tctatttctt ccaagtgggg acagcgctat caaatctgtt attagcatga cagttagact
    38881 cctgttagat gaccaatctt agctctccta aaatcagtgg acactaccat ttcggtagtg
    38941 ttctttttta agctaatctc atcaccacgc tttcttcaag ctaaaaaact gatcatgaaa
    39001 gagcaacccc atcaccctaa cagcccactt tgcccgcact ttcttaaaaa gttaggagaa
    39061 aacgatatac caattatttt ctgatcccag ctccctatca ttaacaagat gtgtagcacg
    39121 gacctacata caggtaagct agagtcattc tcgctatttc ggacaaaaag aggccggtag
    39181 ttccctgacc tcttctttat ttatcttgta acactcctct tcttagcgag acttactagc
    39241 acataattct taagcactct tatcatttag actaggaata acaccatctg aatgaagcaa
    39301 atgatgcgcc agatctgcca gagtatcatc tccataagta atccctgttt ggtgctgttt
    39361 ctgatgaaaa tcagatgact ccttaacttt agcagtcacc tgctggtcac ttggatgaaa
    39421 attaatttct ctcttggctt cagccgcaac tggctcgagg aacaacttat tgacattacg
    39481 gtcttcctcc aaatgattaa ggatagcagc atcatcttct aacgaaagaa ccttcttggg
    39541 taacaaaaag gggttattta gccttggctc agtaacgact tccttggctg ctcgtgatag
    39601 tttattattg tcttcctcag gtatcaaacg attaagcaat cgatgcaatt cttccggcta
    39661 tgaggcgctc acagctactt cagaagaaac ctccaagccc tttcctgttt gcctgtctaa
    39721 agcaaccggc aaattattaa ctaacttttt aacctgtcct cagcgctcat ttcaacacta
    39781 ggaagagctg tcgctggttc tagccagcta ccaacttctg ctggtcttac cttaacatcg
    39841 ctggcaggct tctccttctc atcttcatta ggtctaaaag gctcaaaccg ccctgttgat
    39901 ttttcatcta tgacctttat attgtctttt actacatgag acagtggttc gacatttacc
    39961 atagcttcat gctcgttaag gctctggtct cgtttgaatt taccaggaat accaggagtc
    40021 ggtgttccaa cagattgatc ggcttcagta ggctctttta cggagccttt tttccataaa
    40081 actgctggag gaataatatc cgtagagcca tccccataag taatcgtggc ctgcctcccc
    40141 tttggattaa tagatacttt aatattgtct gggaaagccc cactacctcc tgtggttaaa
    40201 agggcacccg catttttaat gagagttgtc attcgttcaa taccagcaac gccaatatgg
    40261 ttcaagtcat ctactggtaa ctgatcaaca gatttcatgg tgtatttcca agcttgcggt
    40321 tgaacaagaa aaacagttcc tttatacatt gtgttatgca tatcatcaat gactttgaaa
    40381 taccttgtat atttggtttg tacattaggg ttagcaacgg gaacagtgct ttcaggaact
    40441 cctgacatcg taaaaatccc tctagcagaa gtttgaaagc cctcataaac atgacccata
    40501 ccaggaacag tctgagcggt caaagactta ccagaaccat ccaaagcaat gcgaatatct
    40561 ttcatcagat acatctgatt ggtataggta aacctaaccg tgttagcctc acctcgatac
    40621 aaaaccactt gggagggctg atccgaagaa aactgagagg ttgtttcttc cttatagcca
    40681 actccaaaac gtctatcaga cgaatggact gaatctgccg caactgtact actcaaactg
    40741 actagtaaag tagccgcaca accaactaat aaactgctcg ttttaacttt aacatctctt
    40801 aacatcatca tgttcctttc attatttttt atttaggaat tcgtcatact atattttctc
    40861 atattagtct aacaaaaaac tcgtaattct atataaatca tataaaatga cgagctttgg
    40921 caaagatgag tatagcagga tggaaaacag attgtttctg aaacttcaaa ggtatcttct
    40981 tgtcacgaac tattctttaa ttgaatcggt tacactaaac tagtcccatt atttaaaaac
    41041 aaactttaaa tattattcat ttcacttttt aagtttatta aatatagagt gattaggaga
    41101 aataagacga cctaatgata aaatcattcc tattgtatag caaacaacag taaggtgtaa
    41161 ataaaagtaa attgatacct ctg
//
LOCUS       DAUDYX010000011        40765 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.011, whole genome shotgun sequence.
ACCESSION   DAUDYX010000011 DAUDYX010000000
VERSION     DAUDYX010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40765
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.011"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            134..295
                     /gene="sagA"
                     /locus_tag="VHD02_000563"
     CDS             134..295
                     /gene="sagA"
                     /locus_tag="VHD02_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM family cytolysin streptolysin S"
                     /protein_id="HEQ5497195.1"
                     /translation="MLKFTSNILATSVAETTQVAPGGCCCCCTTCCFSIATGSGNSQG
                     GSGSYTPGK"
     gene            517..1467
                     /gene="sagB"
                     /locus_tag="VHD02_000564"
     CDS             517..1467
                     /gene="sagB"
                     /locus_tag="VHD02_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin S biosynthesis dehydrogenase SagB"
                     /protein_id="HEQ5497196.1"
                     /translation="MSFFTKEQQPKENCPPITVEKARQLFEFNTNHLSLSDYHHQTVL
                     KTSKQLVAQHLMPNATDNLSQHFLMNYKANNNYLGFQASIVDFFTDSAVANFSSSYVY
                     ESQEKIIRLPKPTKISTALSTCIIKRRSHRQFSDRQMPLQDLSNILYYACGVSSQASI
                     RDGASDKITLRNCASGGGLYPIHLVFYARNISKLIDGFYEYLPYQHALRCYRHSSEEN
                     VRDFAEYGAINAENCNIIIIYVYHYIKNTRKYGNQATAYAFIESGEIAQNIQLTATAL
                     TYGSIDIGGYNKEYLQELLDLDGLGEHVIHMTLVGTKESQ"
     gene            1464..2522
                     /locus_tag="VHD02_000565"
     CDS             1464..2522
                     /locus_tag="VHD02_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagC"
                     /protein_id="HEQ5497197.1"
                     /translation="MKYQLNSNVRVVTFQDTFCFRKGIWDFNEAILDLTQEPQNLKIV
                     YQEIVSQLVKGVAIDTETYENTLEPETFANLMEVISGLYYNDMLMLEDDYNLEENVMK
                     ILMGNFRFMAQAGHMVSNDPVLFISDSTYVNESAELLAEQLHLQLQVASDDLKMLIQQ
                     TDVSSRLDALEHRRNMNCLSEALCDYQSIIICQERLNIMMLRHLNEVSVAMKKQLVIG
                     FVDGPFLHTCTLNPPHSADFDSLERRVLARLQDSTLYQHFANQILPATQDVSQAYLPL
                     LNVLMNLVVSEAFIIAQTGSSKFEGRLLSIYLPTLEIQVQDILKMSNSQTQGALAKLK
                     YEDQQISTREIVKKLLDE"
     gene            2542..3900
                     /locus_tag="VHD02_000566"
     CDS             2542..3900
                     /locus_tag="VHD02_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEQ5497198.1"
                     /translation="MLYYYPSFNHIFDELKSLSGNRTGILNQSQVPVCNHPHDVYLKS
                     ITGQMPDYHKQFIGELSQVSYHIIGYGSYYEEALIKYLGESIERYATVIAGDLLSDRI
                     VYASYNELKLLHKVMPLEYLQVFTQEQIALSCDLQMMMCDKMVTENDVLGWVKCPMFF
                     EDAEMYVPAQMLCVGYKTNETVGERRIIPGFSTGTASHKTLEAAMCNSLIEYIQIDSM
                     MLSWYTKKPCPKIIVDDPDIEVILEEARLGKDSLYDIIPIDMTVGEDNPLYTFGIILK
                     NKYDEGPYLLFGVQAGLDPKHALLRGIMEASAISYSYYYNLLYQKASLANIECEEPLF
                     LDLDSNVFYYAHPKDQDHKWKAFEPLISGEVLLSDLEDHSGKDKKEDLKTLLAYAKKV
                     SPNAVFLDITPPEALEKGWYVTRVLMPELLEMCIPAFPFANHPRMRQFGGVTNAFVHP
                     MP"
     gene            3875..4546
                     /locus_tag="VHD02_000567"
     CDS             3875..4546
                     /locus_tag="VHD02_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEQ5497199.1"
                     /translation="MPLSIQCLNLCFLLVTFCPSIPMQAIFGKEDSGYAFNLIGFLRA
                     TLIYDILALVSIYVLSPQITLSLESIDSKTFFMGLVFCVLIVLIELVFLHGLRCWQKK
                     QWLPATFSFVGTTNDWSKIGYPLLLALFEETIYRFLWFNILAFQWYLPTIIVLIVTSF
                     CYALNHLLMGKSIFYAKLVTGIIYGSIYMLTSQLWLVVIMHVGGNLLVECLSHLQTKK
                     KKEVT"
     gene            4543..5226
                     /locus_tag="VHD02_000568"
     CDS             4543..5226
                     /locus_tag="VHD02_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagF"
                     /protein_id="HEQ5497200.1"
                     /translation="MMLLVLLSFLGLTTLWLTSYRRRCLARWHLQWLVALSYQDFLDV
                     LLSLFQFVIIILVLFFYSATINLGEVLTFLTQTSWHWQILCYLVLYLMAIIEMTLLVL
                     ILIFDVLLQKDSRLLFKKITWLPFRQDKPVLSILLLGIVILVDSLFYLGLLILLGEQS
                     LTSLATLILGYGIVKACRYSGWLNVLLAFCLFTIVGLWAVTATLLYGWLVGAMILTVT
                     YLMISCKEY"
     gene            5249..6172
                     /locus_tag="VHD02_000569"
     CDS             5249..6172
                     /locus_tag="VHD02_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5497201.1"
                     /translation="MSFVQLTNVVKSYKNGKKAVNDVSLSIEAGNIYGLLGPNGAGKS
                     TLINLILGLIPLSSGKITVLGQSQKTIRKISSQIGYVPQDIAVYPDLTAYENVELFGS
                     LYGLKGAQLKKQVLKSLEFVGLHSQAKQFPSQFSGGMKRRLNIACALVHSPKLIIFDE
                     PTVGIDPQSRNHILESIRLLNKEGATVIYTTHYMEEVEALCDYIFIMDHGQVIEEGPK
                     FELEKRYVANLANQIIVTLTDSRHLELADKPDWSLIEDGEKLMLKIDNSDMTSVVHQL
                     TQANITFSEIRHNHLNLEEIFLHLTGKKLRD"
     gene            6181..7308
                     /locus_tag="VHD02_000570"
     CDS             6181..7308
                     /locus_tag="VHD02_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ5497202.1"
                     /translation="MVLFHLIKKESLQIFRNRTALLMMVIFPILMIVILSFAFKSSFN
                     TATTVPKLTIRYQLEGEKTDYQKNFLAFLKVLNQKLHLEAKPSNSLEKDRQRVSEGAL
                     TAVLEVKKNQTIKVITNNINQQNADLINMLVKNYVDNAKTYDSIAALYPQQLNHIRKR
                     SVDYVKVSSIQTSKGMTSADYYAISMFTMITFYSMMSAMNLVLSDRQQRITNRIHLTG
                     VSPSLLVFGKLIGAMLATTVQLSLLYIFTRFVLRVNWGTNEWMLIGITASLVYLSVAI
                     GIGLGISIKNEAFLTVASNTIIPIFAFLGGSYVPLTTLHSSIINQLSNISPIKWVNDS
                     LFYLIFGGQYNPIPVTLIVNISIGTIFIILSLIGMRKQVTT"
     gene            7305..8423
                     /locus_tag="VHD02_000571"
     CDS             7305..8423
                     /locus_tag="VHD02_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ5497203.1"
                     /translation="MICFIKTLFVKIKRKKTSYVTFFLMPILTTLLALSLSFSNNNQA
                     KIGILDKDNSQISKQFIAQLKQNKKYDIFTKIKKEHIDHYLQDKSLEAVLTIDKGFSD
                     KVLQGKSQKLNIRSIANSEITEWVKAQTNYLLENYNIIGDVALGNEDTFNRILQKNQQ
                     LNYDVKQVTLTDRSRSKAVSSTTTGFLLILMLGSTSVIYSGILADKSSQLYHRLMLSN
                     LSRFRYMLSYVCVGFVAFTIQIVIMLSLLKVFNISFFVPTSLLLIIFFLFSLLAIGFG
                     LLIGAITQNSQQSSQLANLIVMPTSMLAGCLWPLSITPSYMQAIGKLLPQNWVLSAIA
                     IFQSGETLSQAWPYLLALMGTALALISFSSLLLKPTKL"
     gene            8994..11726
                     /locus_tag="VHD02_000572"
     CDS             8994..11726
                     /locus_tag="VHD02_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpnA family nuclease"
                     /protein_id="HEQ5497204.1"
                     /translation="MINKKYIIPVSLLTLAITLTSVEEVTSRQNLTYANEIVTQRPKR
                     ESVISDKSNFPVISPYLASVDFGERKTPLPTPDKGVKVTTEQSIAQVRKGPEERTYTV
                     TGKITSVINGWGGYGFYIQDSEGIGLYVYPEKDLGYSKGDIVQLTGTLTRFKGDLQLQ
                     QVTAHKKLELSFPTSVKEAVISELETTTPSTLVKLSHVTVGELSTDQYNNTSFLVRDD
                     SGKSIVVHIDHRTGVKGADVVTKISQGDLINLTAILSIVDGQLQLRPFSLEQLEVVKK
                     VTSSNSDASSRNIVKIGEIQGASHTSPLLKKAVTVEQVVVTYLDDSTHFYVQDLNGDG
                     DLATSDGIRVFAKNAKVQVGDVLTISGEVEEFFGRGYEERKQTDLTITQIVAKAVTKT
                     GTAQVPSPLVLGKDRIAPANIIDNDGLRVFDPEEDAIDYWESMEGMLVAVDDAKILGP
                     MKNKEIYVLPGSSTRPLNNSGGVLLPANSYNTDVIPVLFKKGKQIIKAGDSYKGRLAG
                     PVSYSYGNYKVFVDDSKNMPSLMDGHLKPEKTNLQKDLSKLSIASYNIENFSANPSST
                     KDEKVKRIAESFIHDLNAPDIIGLIEVQDNNGPTDDGTTDATQSAQRLIDAIKKLGGP
                     TYRYVDIAPENNVDGGQPGGNIRTGFLYQPERVSLSDKPKGGARDALTWVNGELNLSV
                     GRIDPTNAAWKDVRKSLAAEFIFQGRKVVVVANHLNSKRGDNALYGRVQPVTFKSEQR
                     RHVLANMLAQFAKEGAKHQANIVMLGDFNDFEFTKTIQLIEEGDMVNLVSRHDISDRY
                     SYFHQGNNQTLDNILVSRHLLDHYEFDMVHVNSPFMEAHGRASDHDPLLLQLSFSKEN
                     DKAESSKQSVKAKKTSKGKLLPKTGDSLVYVITLLGTASLLVPILLLTKGKKES"
     regulatory      11891..12006
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            12004..12504
                     /locus_tag="VHD02_000573"
     CDS             12004..12504
                     /locus_tag="VHD02_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEQ5497205.1"
                     /translation="MTKLTVHDYVHIGLVAALYVVLTITPPLNAISYGMYQFRISEMM
                     NFLAFYHRKYIIAVTLGCMIANFYSFGLIDVFVGGGSTLIFVTLGVILFSKYQKDYLF
                     NGIFNKAFVYFSFFFATSMFTVAIELYFFGAPFLLTWFTTALGELVSLLIGSLIIDKL
                     SQRISF"
     gene            12698..14656
                     /gene="ligA"
                     /locus_tag="VHD02_000574"
     CDS             12698..14656
                     /gene="ligA"
                     /locus_tag="VHD02_000574"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEQ5497206.1"
                     /translation="MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQ
                     SYPEYVLQDSPTQQVGGTILKGFEKYRHQYPLFSLQDAFSREELDAFDKRVKAEFPNA
                     TYLAELKIDGLSISLSYENGFLQVGATRGDGNIGENITENIKKIKDIPHQLSEPLTIT
                     VRGEAYMSRQSFKAINEARQENGETEFANPRNAAAGTLRQLDTSVVAKRQLATFLYQE
                     ASPTARNQQNEVLAELAGLGFSVNPYYQLTSSMDEIWDFIKTIEAKRDQLAYDIDGVV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPSLIQRSLEHFASRDAMNITGLGPAIVEKLFL
                     AGFVHDVADIYQLTKENFMQLDGIKEKSADKLLAAIEASKSNSAEKLLFGLGIRHIGS
                     KVSRLILEVYGDISALLTAKEEEIARIDGLGSTIAQSLTQYFEQKTAAILVDELKTAG
                     VNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNEAKDKLEALGAKVTGSVSKKTDLVI
                     AGSDAGSKLEKAKSLGIRIEDEDWLRQL"
     gene            14670..15692
                     /locus_tag="VHD02_000575"
     CDS             14670..15692
                     /locus_tag="VHD02_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEQ5497207.1"
                     /translation="MKKQLRARLIYNPTSGQELMRKSVPEVLDILEGFGYETSAFQTT
                     AQKNSALNEARRAAKAGFDLLIAAGGDGTINEVVNGIAPLKKRPKMAIIPTGTTNDFA
                     RALKVPRGNPSQAAKLIGKNQTIQMDIGRAKKDTYFINIAAAGSLTELTYSVPSQLKT
                     MFGYLAYLAKGVELLPRVSNVPVKITHDKGVFEGQVSMIFAAITNSVGGFEMIAPDAK
                     LDDGMFTLILIKTANLFEIVHLLRLILDGGKHITDRRVEYIKTSKIVIEPQCGKRMMI
                     NLDGEYGGDAPITLENLKNHITFFADTDLISDDALVLDQDELEIEEIVKKFAHEVEDL
                     EQELEE"
     gene            16084..16281
                     /locus_tag="VHD02_000576"
     CDS             16084..16281
                     /locus_tag="VHD02_000576"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEQ5497208.1"
                     /translation="MNPIFALALACFGVSLAEGFLMANLFKAASRQPEIIGQLRSLMI
                     LGVAFIEGTFFVTLVMAFILK"
     gene            16316..17032
                     /gene="atpB"
                     /locus_tag="VHD02_000577"
     CDS             16316..17032
                     /gene="atpB"
                     /locus_tag="VHD02_000577"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEQ5497209.1"
                     /translation="MEEAKIPMLKLGPITFNLTLLAVCIVTIAVIFAFVFWASRQMKL
                     KPEGKQTALEYLISFVDGIGEEHLDHNLQKSYSLLLFTIFLFVAVANNLGLFTKLETV
                     NGYNLWTSPTANLAFDLALSLFITLMVHIEGVRRRGLVAHLKRLATPWPMTPMNLLEE
                     FTNFLSLAIRLFGNIFAGEVVTGLIVQLANYRVYWWPIAFLVNMAWTAFSVFISCIQA
                     FVFTKLTATYLGKKVNESEE"
     gene            17050..17544
                     /gene="atpF"
                     /locus_tag="VHD02_000578"
     CDS             17050..17544
                     /gene="atpF"
                     /locus_tag="VHD02_000578"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEQ5497210.1"
                     /translation="MSITFGELVGNFILVTGSVIVLLLLIKKFAWGAIESILQTRSQQ
                     ISRDIDQAEQSRLSAQQLEAKSQANLDASRSQASKIISDAKEIGQLQGDKLVAEATDE
                     AKRLKEKALTDIEQSKSDAISAVKTEMSDLTVLLAEKIMGANLDKTAQSQLIDSYLDD
                     LGEA"
     gene            17544..18080
                     /locus_tag="VHD02_000579"
     CDS             17544..18080
                     /locus_tag="VHD02_000579"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEQ5497211.1"
                     /translation="MTKKEQALIEQYAKSLVEVASEHHSLDALQADVLAILETFVTTN
                     LDQSLSSLAVPHAEKIKLLTLLKGNNSVYMNNFLNLILQNEREAYLYQMLQAVLNEIA
                     IVSNQYDVTVTSSLPLTEEQKSRVRAVVAKKFAVTAGRLIEKVDPSLIGGFIISVNNK
                     VIDTSIRRQLQAFKMNLK"
     gene            18096..19604
                     /locus_tag="VHD02_000580"
     CDS             18096..19604
                     /locus_tag="VHD02_000580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEQ5497212.1"
                     /translation="MAINAQEISALIKKQIENFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFENGAYGMAQNLESNDVGIIILGDFSAIREGDVVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPVDGLGDIETTGFRPVETPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRRYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDDLGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     DDILAFEEALYDYFDVHYNDLFETIRTTKDLPEEAALDAAIKAFKEHSNFKS"
     gene            19620..20495
                     /locus_tag="VHD02_000581"
     CDS             19620..20495
                     /locus_tag="VHD02_000581"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEQ5497213.1"
                     /translation="MAGSLSEIKAKIISTEKTSKITSAMRMVSSAKLVKSEQAARDFQ
                     IYASKIRQITTDLLKSELTIGSDNPMLVSRPVKKTGYIVITSDKGLVGGYNSKILKSV
                     MDMITEYHADGDYEIISIGSVGSDFFKARGMNVAFELRGLADQPSFEQVRQIISQSVD
                     MFVNEIFDELYVCYNHHVNSLTSQVRVQQMLPISDLVADEAAEEGVTGFELEPNRHDI
                     LDQLLPQFTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQAAI
                     TQEITEIVAGANALE"
     gene            20657..22063
                     /gene="atpD"
                     /locus_tag="VHD02_000582"
     CDS             20657..22063
                     /gene="atpD"
                     /locus_tag="VHD02_000582"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEQ5497214.1"
                     /translation="MSSGKIAQVVGPVVDVMFASGDKLPEINNALIVYKDSDKKQKIV
                     LEVALELGDGMVRTIAMESTDGLTRGLEVLDTGRAISVPVGKETLGRVFNVLGETIDL
                     EEPFAEDVDRQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTQKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALSPEIVGEEHYAVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAEQFTGLPGSYVPVAETVRGFKEILEGKYDDLPEDAF
                     RSVGPIEDVIKKAEKMGF"
     gene            22076..22492
                     /locus_tag="VHD02_000583"
     CDS             22076..22492
                     /locus_tag="VHD02_000583"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEQ5497215.1"
                     /translation="MTQMTVQVVTPDGIKYDHHAKCISVTTPDGEMGILPNHINLIAP
                     LQVHEMKIRRGGEDEKVDWIAINGGIIEIKDNVVTVVADSAERDRDIDVSRAERAKLR
                     AEREIAQAETTHNIDEVRRAKVALRRALNRINVSKK"
     gene            22758..23015
                     /locus_tag="VHD02_000584"
     CDS             22758..23015
                     /locus_tag="VHD02_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEQ5497216.1"
                     /translation="MVCYNNPMELLMKIIAFSCHLFFIGLSYQLLISVIDWTKFSHYH
                     PENLGRLRLFVFLLATALGYLVSHCMLELLQISQTLFFEFC"
     gene            23080..24351
                     /gene="murA"
                     /locus_tag="VHD02_000585"
     CDS             23080..24351
                     /gene="murA"
                     /locus_tag="VHD02_000585"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEQ5497217.1"
                     /translation="MDKIIIEGGQTRLEGEVVIEGAKNAVLPLLAASILPSKGKTILR
                     NVPILSDVFTMNNVVRGLDIRVDFNEAANEITVDASGHILDEAPYEYVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPINLHLKGLEAMGATITQKGGDITAQADRLQGAMI
                     YMDFPSVGATQNLMMAATLADGVTTIENAAREPEIVDLAQFLNKMGARIRGAGTETLT
                     ITGVTSLHGVEHDVVQDRIEAGTFMVAAAMTSGNVLIRDAVWEHNRPLISKLMEMGVS
                     VTEEEYGIRVQANTPKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMVETVFEN
                     RFQHLEEMRRMGLQSEILRETAMIHGGRQLQGAPVMSTDLRASAALILTGIVAQGVTI
                     VNNLVHLDRGYYQFHEKLAKLGATISRSSEV"
     gene            24355..24543
                     /locus_tag="VHD02_000586"
     CDS             24355..24543
                     /locus_tag="VHD02_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEQ5497218.1"
                     /translation="MTNGLKYVLEQMLLLLVIAALACLFLAIGLMIGYSFMGDGHSPW
                     HILSMDKWAELVNKFTGK"
     gene            24579..25118
                     /locus_tag="VHD02_000587"
     CDS             24579..25118
                     /locus_tag="VHD02_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEQ5497219.1"
                     /translation="MLTRKKRKRVQEQKVPKRANALLTKSTRQYQDRSQLKKGYRSFK
                     PAGWHRLHHLKGSYDHAVDRGHLLGYALVGGLKGFDASTGNPDNIATQLSWANQANKP
                     YSTGQNYYEGLVRRALDKGHRVRYRVTLLYDGDNLLASGSHLKAKSSDDSLAFNVFVP
                     NVQAGLTVDYRTGQIAINL"
     gene            25401..26444
                     /gene="pheS"
                     /locus_tag="VHD02_000588"
     CDS             25401..26444
                     /gene="pheS"
                     /locus_tag="VHD02_000588"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019314825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEQ5497220.1"
                     /translation="MDLQAQLEELKSKTLETLQSLTGNHTKELQDLRVAVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDLLTKAFEEQAKIVEAAKIQAQLDAESIDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTLDQHDFSKGPLKMVSPGRVFRRDTDDATHSHQFHQI
                     EGLVVGKNISMGDLKGTLEMIIKKMFGEERSIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKKTGWIEILGAGMVHPSVLEMSGVDAKEYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDQRFSEQFN"
     gene            26654..29059
                     /locus_tag="VHD02_000589"
     CDS             26654..29059
                     /locus_tag="VHD02_000589"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEQ5497221.1"
                     /translation="MLVSYKWLKELVDIDVTPVALAEKMSTTGIEVEGIEVPAEGLSK
                     LVVGHVLSCEDVPETHLHLCQVDTGDETPRQIVCGAPNVKAGIKVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSDSIIPKEFSDGIQILPEEAVPGDAIFKYLDLDD
                     HIIELSITPNRADALSMRGVAHEVAAIYGKSVSFPQKNLQESDKATSEAIELSIASDN
                     VLTYASRVVENVKVKPSPQWLQNLLMNAGIRPINNVVDVTNYVLLYFGQPMHAFDYDK
                     FEDHKIVARAARQGESLVTLDGEKRDLTTEDLVITVAEKPVALAGVMGGQATEIDGNS
                     QTVVLEAAVFDGKSIRKTSGRLNLRSESSSRFEKGVNYATVLEALDFAAAMLQELAEG
                     QVLSGHVQAGQLPTEPVEVSTSLDYVNVRLGTELTFADIQRIFDQLGFGLTGDETSFT
                     VAVPRRRWDVSIPADLVEEIARIYGYDKLPTTLPEAGGTAAELTPTQALRRKVRGLAE
                     GLGLTEIISYALTTPEKAVEFAVAPSHLTELMWPMSVERSALRQNMVSGMLDTVAYNV
                     ARKQSNLALYEIGKIFEQEANPKEDLPNEVNHFAFAICGLVAQKDFQTQAQAVDFYHA
                     KGILDTLFANLNLKVQYVPTKDLANMHPGRTALILLDEQVIGFVGQVHPGTAKAYSIP
                     ETYVAELDMAALEAALPSDQTFAEITKFPAMTRDVALLLDREVSHQAIVTAIESAGVK
                     RLTSIKLFDVYEGATIQAGKKSMAYSLTFQNPNDNLTDEEVAKYMEKITKALTEQVGA
                     EVR"
     gene            29169..29546
                     /locus_tag="VHD02_000590"
     CDS             29169..29546
                     /locus_tag="VHD02_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEQ5497222.1"
                     /translation="MYQTKITGNRLYHTVSKGYGDSVTLFGKTDQGETPMSLLVIALS
                     SCVTMCVQGFYHRQFQQDTVAVSVEASYEEEVFELLVRIDDVLDDKKKADLLAYIGTY
                     CRVKQLLKPEVTVTITLEGLSHV"
     gene            29539..29925
                     /locus_tag="VHD02_000591"
     CDS             29539..29925
                     /locus_tag="VHD02_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEQ5497223.1"
                     /translation="MSDASLKTYQKQWAYQKYWVMAHSQQHYNALRELFKGNQWSEEK
                     VLTFHCLIEEAQAIPPTVKSLRTAYQHVWGYFKKVASQEEKDHFKDLDAQLETKSEEM
                     LCFLQEMTAHYQPSYLLSCRLITKGP"
     gene            29998..31074
                     /locus_tag="VHD02_000592"
     CDS             29998..31074
                     /locus_tag="VHD02_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ5497224.1"
                     /translation="MFLALNEMKQSKLRYGLIAGLLCLVAYLMFFLSGLAFGLMQENR
                     SAVDLWKADSVLLAKDADATLTLSQVSRAQENQITADKVAPLAQLNTVAWSVKNPKDA
                     DKVKVRLFGIDSNSFIRPNIVKGRLFKTNKEVVLDQSLAKEEAFAIGKDFYTSSSSQA
                     LTIVGYTQNARFSVAPVVYMNLEAFETLKYGEPLPKDKQVVNAFITKGSLTDYPKKDF
                     QKLDIKTFITKLPGYSAQLLTFGFMISFLVIISAIIIGIFMYILTIQKAPIFGIMKAQ
                     GIGNITIAQAVLLQTFLLSALGSGLGLLGTWLTSLVLPEAVPFQSNWLVYLAILVSMV
                     CFALLGTLFSVFSIVRIDPLKAIG"
     gene            31084..31752
                     /locus_tag="VHD02_000593"
     CDS             31084..31752
                     /locus_tag="VHD02_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5497225.1"
                     /translation="MSVLTFKQVTKTFQDGHHEINALKATDFSIEAGEFVAIIGPSGS
                     GKSTFLTIAGGLQTPSSGQLIIDGTDYTHLSEKERSRLRFKSVGFILQASNLIPFLTV
                     QQQLELVDHLTGSKEKAKANQLFDDLGIAGLKHQLPQELSGGERQRAAIARALYHDPA
                     LILADEPTASLDTEKAYEVVKLLAKESKEKNKAIIMVTHDDRMLKYCDKVYRMQDGEL
                     RQER"
     gene            complement(31856..32773)
                     /locus_tag="VHD02_000594"
     CDS             complement(31856..32773)
                     /locus_tag="VHD02_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEQ5497226.1"
                     /translation="MKTDDLRESQQVEDRRGQSSGSFGGGGLGGGLLLQLLFSRGGWK
                     TKLVILLLLLVMGGGGLSGVLGGKPSSTNNNAYQSSQVTRTNGDKASQEQVSFVSKVF
                     ASTEDYWTKTFREKGLTYHKPTLVLYTGATQTACGRGQASSGPFYCPGDQKVYLDISF
                     YNELSTKYGAKGDFAMAYVIAHEVGHHIQNELGIMDNYASTRQGKSKAKANQLNVKLE
                     LQADYYAGAWANYVQGQGLLEKGDIEEAMAAAHAVGDDTLQEETYGRAVPDSFTHGTS
                     KQRQRWFDRGYQYGDFEHGDTFSIPYSKL"
     gene            32924..36139
                     /gene="rexB"
                     /locus_tag="VHD02_000595"
     CDS             32924..36139
                     /gene="rexB"
                     /locus_tag="VHD02_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEQ5497227.1"
                     /translation="MKLIYTEMSYSMTEILVNEARKAADQGYRVFYIAPNSLSFEKER
                     EVLTLLPERGTFSIIVTRFVQMSRYFTVESSPSKQHLDDTTLAMIFYRALMQLKPEDL
                     PSYGRLQNNSVFIEQLVELYKELKNAQLSVHDLTGLDHPQKQEDLIKIIELAETIMIQ
                     QDYNQDSPLQSFARAIKLGLLNNQLSKTVIVIDGFSRFSAEEDYLLSLLNNNCQEVII
                     GSYVSQKAYQKSFIKGNIYEASLHFLQDLAQKYHIKPVFATSNQVFKPAFSRLTQLFE
                     ATHDFSQVDWQLQKNDLDHFSLWQCHHQKEEIEHVAKSIRQKLYEGYRYKDILVLLGD
                     MDAYQLQIGPIFDKFEIPYYLGKAEPMAAHPLVQFIESLERSQRYNWRREDILNMLKS
                     GLFGCFDDSDIDRFEEYTQFADIKGFTKFSKPFTINSSRQYPLDFLNEMRQDIVLPLQ
                     ELFKSQKQLGASLIDKLILFLEKIRLAENMQGLAQSQLEVEKNEEVWKRFTDILTSFH
                     HIFGQEKLRLSDCLALIKTGMKSAQYRVVPATLDVVTIKSYDLVQPHSKPFVYAIGLT
                     QSHFPKQIHHSGLLSDQERARINEIRNYRHFDIASAENSKKNHQTALSLFNAATKELV
                     LSVPTVINETFDDLSPYLKELINFGLPLLDKGKNYLSYDNSDIGNYKALLSQIIAINR
                     QDLIEMSDQDKMFWTVVLRYLRKQLRKQQLELPTSDYRLSTKPLSKEVIEVCFPKGIP
                     LKLSATALTVFYNNQYNYFLKYVLNLNKTESIHPDSRIHGQYLHRVFERLMKDHTQEP
                     FDNKLKQAIYHTNQESFFQQVYQDNAEAEYSLAILEDIVRSTAPILQLNQNIKVIDQE
                     KNFHLDMGNEILVHGIIDRIDQLSDGSLGIVDYKSSANQFDIGTFYNGLSPQLVTYLA
                     ALKQIAPHDINQLFGAMYLHLQDPKLDLVTFKQIDNTLVESIYKALTYKGIFSEVEKE
                     HLSTGAYQTKNALYSNDELETLLNYNKYLYLKAAKHIKKGHFLINPYTSDGKTVQGDQ
                     LKAITRFEADLDMAQARRLVTLPAKEKKECFLTLMRKESHL"
     gene            36100..39768
                     /gene="addA"
                     /locus_tag="VHD02_000596"
     CDS             36100..39768
                     /gene="addA"
                     /locus_tag="VHD02_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEQ5497228.1"
                     /translation="MLFNINEKGEPLVISFAPFLSPEAIKHLQENERCSDQSQKRTAQ
                     QIEAIYTSGQNILVSASAGSGKTFVMVERILDKILRGVSIDRLFISTFTVKAATELRE
                     RIENKLYSQIAQTTDFQMKVYLTEQLQSLGQADIGTMDAFAQKVVSRYGYSIGISSQF
                     RIMQDKAEQDVLKQEVFSKLFSEFMNQKEAPVFRALVKNFSGNCKDTSAFRELVYTCY
                     SFSQSTENPKIWLQENFLSAAKTYQRLEDIPDHDIELLLLAMQDTANQLRDVTDMEDY
                     GQLTKAGSRSAKYTKHLTIIEKLSDWVRDFKCLYGKAGLDRLIRDVTGLIPSGNDVTV
                     SKVKYPVFKTLHQKLKQFRHLETILMYQKDCFPLLEQLQDFVLAFSEAYLAVKIQESA
                     FEFSDITHFAIKILEENTDIRQSYQQHYHEVMVDEYQDNNHMQERLLTLLSNGHNRFM
                     VGDIKQSIYRFRQADPQIFNQKFRDYQKKTEQGKVILLKENFRSQSEVLNVSNAVFSH
                     LMDESVGDVLYDEQHQLIAGSHAQTVPYLDRRAQLLLYNSDKDDGNAPSDSEGISFSE
                     VTIVAKEIIKLHNDKGVPFEDITLLVSSRTRNDIISHTFNQYGIPIVTDGGQQNYLKS
                     VEVMVMLDTLRTINNPRNDYALVALLRSPMFAFDEDDLARIALQKDNELDKDCLYDKI
                     QRAVIGRGAHPELIHDTLLGKLNIFLKTLKSWRRYAKLGSLYDLIWKIFNDRFYFDFV
                     ASQAKAEQAQANLYALALRANQFEKSGYKGLYCFIKMIDKVLETQNDLADVEVAAPKQ
                     AVNLMTIHKSKGLQFPYVFILNCDKRFSMTDIHKSFILNRQHGIGIKYLADIKGLLGE
                     TTLNSVKVSMETLPYQLNKQELRLATLSEQMRLLYVAMTRAEKKVYFIGKASKSKSQD
                     ITDPKKLGKLLPLALREQLLTFQDWLLAIADVFSTEDLYFDVRFIEDSDLTQESVGRL
                     QTPQLLNPDDLKDNRQSETIARALDMLEAVSQLNANYEAAIHLPTVRTPSQLKAAYEP
                     LLEPIGVDIIEKSSRSLSDFTLPHFSKKVKVEASHIGSALHQLMQVLPLSKPINQQTL
                     LDALREIDSNEEVKTALDLKKIESFFCDTSLGQFFQTYQKHLYREAPFAILKVDPISQ
                     EEYVLRGIIDAYFLFDDHIVLVDYKTDKYKQPIELKKRYQQQLELYAEALTQTYKLPV
                     TKRYLVLMGGGKPEIVEV"
     gene            39908..40720
                     /locus_tag="VHD02_000597"
     CDS             39908..40720
                     /locus_tag="VHD02_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEQ5497229.1"
                     /translation="MNFKKIRFGFLLCLLLYPLAACNKSEQLNHYERIKKTRKLVVAV
                     SPDYAPFEFKALVNGKDTIVGADVQLAQAIADELDVDLELSPMSFDNVLSSLQTGKAD
                     LAISGISHTKERAKVYDFSIPYYQAENAIVMRASDAKVTKNISDLNGKKVAAQKGSIE
                     EGLVKIQLPKANLISLTAMGEAINELKAGQVYAVTLEAPVAAGFLAQHKDLALAPFSL
                     KTSDGDAKAVALPKNSGDLTKAVNKVIAKLDEQERYKSFIAETIALTKNTMK"
BASE COUNT        12786 a         6904 c         8333 g        12742 t
ORIGIN      
        1 gatagttgtt gtgttacaac agtacaattg agctagcctt gtccttgttg tgttaacttt
       61 atttttaaaa taaggttaaa aataaacgac tcgtgttctt atcagttact tattagataa
      121 ggaggtaaac cttatgttaa aatttacttc aaatatttta gctactagtg tagctgaaac
      181 aactcaagtt gctcctggag gctgctgttg ctgctgtact acttgttgct tctcaattgc
      241 tactggaagt ggtaattctc aaggtggtag cggaagttat acgccaggta aataatctat
      301 ttagcatctc tatgtgatag tgatattaag gtaatgagtt tgctagcaac taagttatct
      361 ttggtaactt ctgattatag atgaatggca tagaggtgtt agaaaacatg agacaaaagt
      421 aatcttcttg atgctaacgt ctcaggtaat tagcaggtac tagacagtac ctgctaatta
      481 ctatatgttt agtaaaatga gataggaaag atagttatgt cattttttac aaaggaacaa
      541 caacctaaag agaattgtcc gccaataact gttgaaaaag caaggcaatt gtttgaattt
      601 aatacaaacc acttgtcctt atcggattac catcatcaaa cggtgctaaa aacatcaaag
      661 cagctagttg ctcaacattt aatgcctaat gcaaccgata atcttagtca acattttttg
      721 atgaactata aagctaataa taattattta ggcttccaag ctagtattgt cgactttttt
      781 acagattctg ccgttgctaa tttttcaagt agttacgttt atgaaagtca ggaaaagata
      841 attcgtttac caaaacctac caagatatca actgctctgt cgacatgtat tataaaacga
      901 agaagtcatc gtcaattttc agatagacaa atgcctcttc aagatttatc aaacattctt
      961 tattatgcat gcggtgttag ttcacaagca tcaattagag atggagcatc agataagatt
     1021 acactcagaa actgtgcttc aggtggaggt ttatacccta ttcatttagt tttttatgct
     1081 agaaacatca gtaaattaat agatggtttc tatgaatatc taccctatca gcatgcacta
     1141 aggtgttatc ggcatagctc tgaggaaaac gttagagatt ttgcggaata cggtgctatt
     1201 aatgctgaaa attgtaatat tattattatt tatgtctacc attacataaa aaatacacgt
     1261 aaatatggga atcaggcgac tgcctatgct tttattgaat caggagaaat agcccagaat
     1321 attcaattga ctgcaactgc cttaacttat ggaagtattg atattggtgg ttataataag
     1381 gaatatctcc aagaattatt agatttagat gggctaggag agcatgtgat tcacatgaca
     1441 ctcgtaggaa ctaaggagtc tcaatgaaat atcaacttaa tagtaatgtt cgtgtcgtga
     1501 catttcaaga tacattttgt tttagaaaag gaatctggga ctttaatgaa gccattctag
     1561 atttgacaca agaacctcaa aatttaaaga tagtttatca agaaatagtt tcacagttgg
     1621 ttaagggagt agccattgat actgaaactt atgaaaatac tttagaacct gagacgttcg
     1681 ctaatttaat ggaagtcatt tcaggcttgt attacaatga tatgctgatg cttgaggatg
     1741 actacaatct tgaagaaaat gtcatgaaaa ttttaatggg taatttccgt tttatggcgc
     1801 aagcaggtca tatggtaagc aatgatcctg ttttgtttat cagtgattcg acttatgtta
     1861 atgaatctgc tgagttgcta gctgaacaac tgcatttaca attgcaagtg gctagcgatg
     1921 acttaaaaat gttaattcag cagacagatg ttagttcgcg tttagatgct ttggagcatc
     1981 gccgtaacat gaattgttta tcagaagcct tgtgcgatta ccaaagtatt attatttgtc
     2041 aagaacgctt gaatattatg atgttgcgac acttgaatga agttagcgta gctatgaaaa
     2101 aacaattagt gattggtttt gtagatggac cattcttgca cacttgtact ttgaatcctc
     2161 ctcatagtgc tgactttgat agcttagaaa gaagagtttt ggcacgttta caagactcaa
     2221 ctctctatca acattttgct aatcagattt taccagcaac acaagatgtg agccaggcct
     2281 atttaccatt gttaaatgtt ttaatgaatt tagtcgttag cgaagctttc attattgctc
     2341 aaacaggtag ttctaaattt gagggacgtt tattaagcat ttacttaccg acgcttgaga
     2401 ttcaggtcca agatattttg aaaatgtcta attcacagac acaaggagct ttggcaaaat
     2461 taaaatatga ggatcagcag atatcaacac gtgagattgt taaaaaactc cttgacgagt
     2521 aacctataag gagaatctat tatgttatac tattatcctt cttttaatca tatttttgat
     2581 gaattaaaaa gtttaagtgg caatcgaaca ggtatcttaa atcaatctca agttccagtt
     2641 tgcaaccatc ctcacgatgt ttacttaaaa agtattactg gccaaatgcc agattatcat
     2701 aaacaattta taggtgagtt gagtcaagtt agttatcaca ttattggtta cggcagttac
     2761 tatgaagaag cacttatcaa gtatttagga gaaagtattg aacgctatgc tactgtaatt
     2821 gccggagact tactatcaga tcggattgtc tatgcttctt ataatgagtt aaaactgtta
     2881 cataaagtga tgcctttaga atacttgcaa gtgtttactc aagaacagat cgcattatct
     2941 tgtgacttgc aaatgatgat gtgcgataaa atggtaactg agaacgatgt attgggctgg
     3001 gttaaatgtc ccatgttctt tgaggacgca gagatgtatg ttccagctca aatgctttgt
     3061 gttgggtata aaacaaatga gactgtagga gaaagacgga taataccagg attttctacg
     3121 ggaacagctt ctcacaaaac gttagaagcg gctatgtgca acagtttgat tgaatacatc
     3181 cagatcgatt caatgatgct tagctggtat actaagaagc cttgccccaa gattattgtt
     3241 gatgatcctg atattgaagt gattttagaa gaagctagac tgggaaaaga cagtctatac
     3301 gacattattc ctatcgatat gactgtagga gaagataatc ctctttatac gtttggaatc
     3361 attctcaaaa ataaatatga tgaagggcct tacctcttgt ttggagtgca ggctgggtta
     3421 gatcctaaac atgctttgct aagagggatc atggaagctt cagctatcag ctatagttac
     3481 tactataatc ttttatatca aaaagcttcc cttgcaaata ttgaatgcga agaacctttg
     3541 tttttagatt tagatagtaa tgttttctat tatgcacatc ccaaggacca agaccataaa
     3601 tggaaggcct ttgaaccttt gatttcagga gaagttctgt taagtgattt ggaagatcat
     3661 tctggtaaag ataaaaaaga agatttgaag actttattag cttatgctaa gaaagtcagt
     3721 cctaatgctg tctttttaga tattacacct cctgaagctc tagaaaaagg ctggtacgtc
     3781 acgcgagttc tgatgccaga acttcttgaa atgtgtatac cagcatttcc ttttgctaat
     3841 cacccacgaa tgagacagtt tggaggtgta acaaatgcct ttgtccatcc aatgccttaa
     3901 tctttgtttt ttgttggtaa ctttttgtcc ttcaattcct atgcaagcta tttttggaaa
     3961 agaagatagt ggttatgcat ttaatttaat tggttttcta agagcaactc tgatttatga
     4021 cattttggct ttggtaagta tttatgtttt gtcacctcaa ataaccttgt ctcttgaaag
     4081 tattgatagt aagacgttct ttatgggact tgtattttgc gtcttgatag tactgattga
     4141 actcgttttc ttacatggtt tacgttgttg gcaaaaaaag caatggcttc ctgcaacttt
     4201 ctcgtttgta ggaacgacaa atgattggtc taagattggc tatcctttat tactagcttt
     4261 atttgaagag acgatttatc gttttttgtg gtttaatata ttagcttttc aatggtattt
     4321 accaacaatt attgttttaa ttgtaactag tttttgctat gctttaaatc acttattgat
     4381 ggggaaatcc attttttatg ctaagttggt aacaggcatc atttatggta gtatttacat
     4441 gctgactagt caattgtggt tggttgtgat aatgcatgta ggagggaact tgctagttga
     4501 gtgtcttagc catcttcaaa caaaaaagaa gaaggaggtg acatgatgct attggttttg
     4561 ctgtcgtttt taggtctaac tactctttgg ttgaccagtt atcgaagacg ttgtttggct
     4621 agatggcatc ttcaatggtt agtggcctta tcctatcaag attttttaga tgtcttactg
     4681 agtctgtttc aatttgtcat tattatactt gttttattct tttactcagc tactattaat
     4741 ctaggagagg ttctgacctt tttgacacaa actagctggc attggcagat tctttgttat
     4801 ttagtgttgt atcttatggc catcatagag atgacgcttt tagttttaat tcttatcttt
     4861 gatgttttgc tccagaaaga tagtcgattg ctctttaaaa aaataacttg gctgcctttt
     4921 agacaagata aaccagtctt aagtatactt ttgctaggca tagttattct agtagacagt
     4981 ctcttttatt tgggcttgct tatattatta ggagaacagt cgctaacaag tttagcaacg
     5041 cttattttag gctatgggat agtcaaggct tgtcgctatt caggttggtt aaatgtacta
     5101 ttagcttttt gtcttttcac tatagttgga ctatgggcag ttacggcaac tttattatat
     5161 ggttggctag taggtgcgat gatattaacg gtgacttatt tgatgattag ttgcaaagag
     5221 tattagcaat attaaaaagg agtgatatat gagttttgta caattaacaa atgttgtcaa
     5281 gtcctacaaa aacggcaaga aagctgtcaa tgatgtttcc ttgtctattg aagcaggtaa
     5341 tatttatggt ttgttaggac caaatggtgc tggtaagtct accttgatta atcttatctt
     5401 aggcttgatc cctttgagtt ctggtaaaat tactgtttta gggcaatccc aaaagactat
     5461 tcgaaaaata agttcgcaga taggttatgt tcctcaagac attgctgttt atccagacct
     5521 aactgcttat gaaaatgtag aactatttgg gtcactttat ggcttaaagg gagctcagct
     5581 taaaaaacaa gttctaaaaa gtttagaatt tgtggggcta cactcccaag ctaagcagtt
     5641 tccaagtcaa ttctcaggag gaatgaagag acggttaaat atcgcttgtg cgctagttca
     5701 ttcacctaaa ttaatcattt ttgacgaacc gactgtaggg attgatcctc aatcacgtaa
     5761 tcatatttta gagtcgattc gtttgctaaa caaagaaggt gccacagtta tttatacgac
     5821 ccattatatg gaagaagtag aggctctttg tgattatatt tttattatgg atcatggtca
     5881 agttattgaa gaaggaccta aatttgaact ggaaaaacgt tacgttgcaa atctagcaaa
     5941 ccagattatt gtaactctaa cagattcacg tcatttggaa ctggcagata agcctgattg
     6001 gtctttgata gaagatggag aaaaactcat gttgaagatt gataatagtg atatgacatc
     6061 agttgttcat cagctcacac aggccaatat tacttttagc gagattagac ataaccattt
     6121 gaatttagaa gaaattttct tacacttaac aggtaagaag ttacgagatt agaggttact
     6181 atggttttat ttcatttaat caaaaaagaa agtttacaga tttttagaaa ccgaacagcc
     6241 ttattaatga tggtgatttt tccaattttg atgatcgtta ttttaagttt tgcctttaaa
     6301 tcaagtttta atactgcgac aacagtccct aaattgacca ttcgttacca attagagggt
     6361 gaaaaaacgg attaccagaa aaattttctt gcttttttaa aagttttaaa ccaaaaactt
     6421 catttagagg ctaaacctag taattctctt gaaaaagatc gacaaagggt cagtgaagga
     6481 gccttaacgg ctgttttaga agtgaagaag aatcagacca ttaaggttat tactaataat
     6541 attaatcagc aaaatgcaga tttaatcaat atgctagtaa aaaattatgt agataatgct
     6601 aaaacttatg actcgatagc agctctttat cctcaacaat taaatcatat cagaaagcga
     6661 agtgtggact atgttaaggt cagttcaata cagacaagta aaggaatgac atcagctgat
     6721 tattatgcta tttccatgtt taccatgatt actttttata gtatgatgtc tgcgatgaac
     6781 cttgttttgt cagatcgtca acaacggatt acaaatcgta ttcacttaac aggagtttct
     6841 ccaagtcttt tggtctttgg aaaattaata ggtgctatgt tagcaacaac tgttcaattg
     6901 agtcttttat acatttttac aaggtttgtt ttacgagtta attggggcac taatgagtgg
     6961 atgcttattg gtataacagc ctccttagtc tatctctctg tagctatagg tatcgggcta
     7021 ggcataagca ttaaaaatga ggctttttta acggttgcat ccaatactat tattcctata
     7081 ttcgcctttt taggaggcag ttacgttcca ttgacaacat tacacagctc tattattaat
     7141 caattgtcga atatatctcc tattaaatgg gttaatgata gtttgttcta tcttattttt
     7201 ggtggtcaat ataatccgat tcctgtaact ttgatcgtta atattagtat cggaacaatt
     7261 tttataatat tgtcattgat aggtatgaga aagcaggtga cgacatgatt tgttttataa
     7321 aaactctatt tgttaagata aagcgcaaaa aaacaagtta tgtgacattc tttttaatgc
     7381 ctattttaac gacacttttg gctttatctt tgagtttttc aaataataat caggctaaaa
     7441 ttgggatatt agataaagat aatagtcaga tatctaaaca atttattgct caactaaagc
     7501 aaaacaaaaa gtacgacatt tttaccaaaa tcaaaaaaga gcatattgat cactaccttc
     7561 aggataaatc tctagaagcg gtattgacta ttgacaaagg gttctcagat aaagtgttgc
     7621 aaggaaaatc tcaaaaactc aatatacgtt cgattgctaa tagcgaaatt acagagtggg
     7681 taaaggcaca gaccaattat ctattggaaa attataatat tataggggat gtggctctag
     7741 gtaacgaaga tacttttaat agaattctcc aaaaaaacca acagttaaat tatgatgtta
     7801 agcaagtaac tttgacagac cgatcacgta gcaaagcagt atcttccaca acgacaggat
     7861 ttttgcttat tctaatgtta ggtagtacga gcgtgattta cagtggtatt ttggcagata
     7921 aatctagtca gttataccat cgattaatgt tatcgaactt atctcgtttt cgctatatgt
     7981 tgagttacgt ctgtgtagga tttgttgctt ttacgattca aattgtcatt atgctgagtt
     8041 tgttaaaagt atttaatatt agtttttttg ttccgacatc acttcttttg attatttttt
     8101 tcctttttag tttattagcg ataggctttg gcctgctgat tggagctatc acacaaaatt
     8161 cccaacaaag tagccagtta gctaatctta ttgtaatgcc gacttcaatg ttggctggct
     8221 gtttatggcc attgtctatt acgccttctt atatgcaagc aattggtaag ttattaccac
     8281 aaaattgggt tttatcagcc atagctattt ttcaaagtgg agagacctta tctcaggctt
     8341 ggccatactt gttagctcta atgggaacag cactagcttt gattagtttt tcaagtttat
     8401 tattaaaacc taccaaacta taattagtct tttgttaaaa atactttcct aattagctaa
     8461 ctaccaggta gcccttttat tggcttatat gcaataaaag ggttgctttt tgcttatatt
     8521 ttctctagtg cacttatctt ttgttaaaag cgattgcgat gttactgttg ttactgtgtt
     8581 ttttagagag catattatca aaagcgacct ctctagaaag ttcgtttttg ttgataaatg
     8641 agttgtcaaa gcattttaaa aaaacgtttt caataaacat aaaaactaat gttattaaaa
     8701 aaaggtgtaa aaacgtattt attttatgaa atgtttttgt tacaataagc atgtatcgaa
     8761 tgctataaag ctgtcttgtt cgctatcagg ggtgcttgtg atgttatgtc agatcaaaca
     8821 agtctttaat tgggtgactt aacagattaa aagaaaagat ctaatcgtgt tacttgatag
     8881 tataattaat gcaaacagaa agtgtatccc aattcatagt ttcaggtgga ggttaatatt
     8941 caaaaataaa atcttgttgt tgtcttaaac taatcacatt aaaaggagat atcatgatta
     9001 acaagaaata tataatacct gtttcattgt tgacactagc tattacgctt actagtgttg
     9061 aagaagttac ttcacgccaa aatttgactt atgccaatga aatcgtaaca caaaggccaa
     9121 agagagaatc tgttattagt gataaatcga attttcccgt catatcacct tacctagcaa
     9181 gtgtggattt tggtgagaga aaaacacctt tgccaacacc tgataaagga gtaaaagtaa
     9241 ctactgaaca gtctattgct caagtaagaa aggggcctga agaaagaacc tatactgtta
     9301 ctggcaagat tacgagtgtg atcaatggct ggggaggcta tggcttttat attcaagata
     9361 gtgaaggtat tggactttat gtttatcctg aaaaagattt aggatacagt aagggagata
     9421 ttgttcaatt aacaggtaca cttactcgct ttaaaggtga tttacaactc caacaggtga
     9481 ctgcacacaa aaagttagag ttatcttttc cgacttctgt taaagaagca gtaatatcag
     9541 aattagaaac aacaacaccc tcaacattag ttaagttatc tcacgtgaca gttggagaat
     9601 tatcaactga tcaatataac aacacatctt tccttgtaag ggatgatagt ggtaaaagta
     9661 tagttgttca tatagatcat cgtacagggg ttaaaggggc tgatgttgtt actaaaataa
     9721 gtcagggtga tttgattaac ctcacagcca tattgtctat tgttgatggt cagttacaat
     9781 taagaccgtt ttctcttgaa caattggaag tggttaaaaa ggtcacaagc tcaaatagtg
     9841 atgcttcatc tcgtaatatt gtgaaaatag gcgagattca aggagctagt catacgtcgc
     9901 cacttctcaa aaaagcggtc accgtagaac aggttgttgt cacttattta gacgattcca
     9961 ctcattttta tgttcaagat cttaatggtg atggtgattt agcgacttca gatggtattc
    10021 gtgtttttgc taaaaacgct aaggttcaag tcggcgatgt tttgaccatt tcaggtgaag
    10081 tggaagaatt ctttggtcgt ggttatgagg aacgtaagca gactgacctt accatcaccc
    10141 aaattgtggc taaagcagtg accaaaacag ggacagctca agttccatca ccgcttgttt
    10201 tagggaaaga tcgtatcgcg ccagccaata ttattgataa tgatggcttg cgtgtgtttg
    10261 atccagaaga agacgctatt gattattggg aatcaatgga aggcatgtta gtggcggttg
    10321 atgatgctaa aatccttggt ccaatgaaaa ataaagaaat ttatgtctta cctggctcta
    10381 gtacaagacc gttaaataat tcaggtggag tattacttcc agctaattct tataacacag
    10441 atgtgattcc tgttcttttc aaaaaaggca aacaaattat taaagcagga gactcttaca
    10501 aaggaagatt agctgggcca gtatcttata gctatggtaa ttacaaggtc tttgttgatg
    10561 acagcaaaaa catgccaagt ttaatggatg gtcatctaaa acctgaaaaa acaaacttgc
    10621 aaaaagacct tagcaagtta agcattgctt cttacaatat tgaaaacttc tcagccaatc
    10681 cttcttcaac taaagatgag aaggtcaaac ggattgccga atcctttatt catgatctga
    10741 atgctccaga cattattgga ttaattgaag tccaagataa taatgggccg actgatgatg
    10801 ggacaacgga tgcgacacaa agcgcacaac gcctcattga tgctattaaa aaactaggtg
    10861 gcccaactta tcgttatgtt gatattgctc cagaaaataa tgttgacgga ggtcaaccag
    10921 gtggtaatat tcgaacagga ttcctttatc aaccagagcg cgtcagcctt tctgataagc
    10981 caaaaggcgg tgctcgtgat gctctaactt gggttaatgg agaattaaac cttagtgttg
    11041 gtcgaattga tccaactaac gccgcttgga aagatgttcg taaatcacta gcagcagaat
    11101 ttatcttcca aggtcgtaaa gtcgttgttg ttgcaaatca tttgaactct aagcgtgggg
    11161 ataatgctct ttatggtcgt gtgcaaccag tcacttttaa atctgagcaa agacgtcacg
    11221 tcttggctaa tatgctagca caatttgcga aagaaggggc aaaacaccaa gctaatattg
    11281 tgatgctagg tgactttaat gattttgaat tcacaaagac gattcaatta atcgaagaag
    11341 gtgatatggt taacttggtg agccgacatg atatttcaga tcggtattct tattttcacc
    11401 aaggcaataa tcagaccctt gataatatat tagtttcacg ccatttactt gatcactacg
    11461 aatttgacat ggttcatgtg aattccccat ttatggaagc tcacggacgc gcatcagatc
    11521 atgatccatt gttacttcaa ttatcatttt ccaaagaaaa tgataaggca gagtcttcta
    11581 aacaaagtgt aaaagctaaa aaaacttcaa aaggaaaact gttgccaaaa acaggagata
    11641 gtcttgttta tgtgataacg ctactaggaa cggctagttt attagtgcct attttattat
    11701 tgactaaagg caaaaaggaa tcatagcgat ttgaagaatt ggagttcata aagagtcgtt
    11761 aagggcttga tatacttacg ttagacctta agctaaaatg gatgactata taaaaactaa
    11821 tattgataac aaaagaagat aacaataaga cagctttact tgctaaagac ttttgtcaag
    11881 ctaattgttt tatggtacaa tgtaaccgtt gctttgcaac ccttggtgct tagttccctt
    11941 tcaccaagca tattagaaac gtagatagct tttgagagtt atccagacgt taaaggagaa
    12001 aaaatgacaa aattgactgt acacgactat gttcacattg ggctagtagc tgccttgtat
    12061 gttgtcttaa ctattacccc gcctttaaat gcgatttctt atgggatgta tcaatttaga
    12121 atatctgaaa tgatgaattt cttagctttt tatcatcgca agtatattat tgcggtgact
    12181 cttggatgta tgattgctaa cttttatagt ttcggactga ttgatgtctt tgtaggcggc
    12241 ggttcaactc ttatttttgt gaccttaggt gttattttgt ttagtaagta tcaaaaagat
    12301 tatcttttta atggcatttt taataaagct tttgtttact tttctttctt ttttgcaact
    12361 tccatgttta ctgtagcaat cgaactttac ttctttggag ctccattttt attaacatgg
    12421 tttacaactg cgttgggaga attagtatct ctgctcatag gatcacttat tattgataag
    12481 ttgtctcaaa gaatttcctt ttagtaatgc atcatactca aaagagttgg gtcaataccc
    12541 ttctcttttt tatgttgttt gtaatgttaa aatcgttcta gatagaaata tttaacactt
    12601 tagctaatag taccatatgg agtggccaac gattactttt gatgttgtta tgatatagaa
    12661 atagctcatt tgttatcttt tatgataaaa taaaagtatg aaaaaacgca taaaagaatt
    12721 aacagatctt cttaaccgtt accgttatga ctattatact aaagatgccc catcagtttc
    12781 tgacagtgac tatgataagc tttatcgtga actggtgact ttagagcagt catatcccga
    12841 gtatgtcttg caagatagcc caacgcagca ggttggaggg actattttaa aggggtttga
    12901 aaaatatcga catcagtatc cgctttttag tttgcaggat gctttttcac gtgaggagct
    12961 ggatgctttt gataagcgcg tcaaagccga atttccaaat gccacctatc tagcagagtt
    13021 aaaaattgat ggtttatcta tttccttgag ttatgagaat ggcttcttac aggttggagc
    13081 aacacggggt gatggcaata tcggtgaaaa tatcacagaa aatatcaaaa aaataaagga
    13141 tattcctcac caattgagtg agcctttaac catcaccgtt cgaggagagg cttacatgtc
    13201 tcgtcagtcc tttaaagcga tcaatgaagc acgccaagaa aatggggaga ctgaatttgc
    13261 taatcctaga aatgctgccg ctgggacact aaggcagcta gatacgtcag tggttgctaa
    13321 acgtcaacta gccacttttt tgtatcaaga agctagtccg acagctagaa atcaacaaaa
    13381 tgaggtctta gcagaattag caggtctagg tttttcagta aatccgtatt atcaactcac
    13441 atcgtctatg gatgagattt gggattttat caaaactatt gaggcaaaaa gagatcaatt
    13501 agcttatgac atagatggcg ttgttattaa agttaatagc ctagccatgc aagaagagct
    13561 agggtttacg gttaaagctc cacgctgggc cattgcttac aaatttccag cagaagaaaa
    13621 agaggcagaa atcctttcgg ttgactggac agtgggacga acaggcgtgg tcacacctac
    13681 agcgaatcta actcctgtgc aactagcggg gacaaccgtg agtcgtgcaa ccctacataa
    13741 tgtggattat attgccgaaa aagatatccg tattggcgat acagtgattg tatataaggc
    13801 aggggacatt attcctgctg tcttaaatgt cgttatgagt aagcggaacc agcaagaagt
    13861 catgcttatt cctaaactat gcccatcttg tggtagtgaa ttagttcatt ttgaagatga
    13921 ggttgctttg cgttgtatta atccgttgtg tcctagtttg attcagcgaa gtttggagca
    13981 ttttgcaagt cgtgatgcca tgaacattac tgggctggga ccagctattg tggaaaaact
    14041 atttttagcg gggtttgttc atgatgttgc tgatatttac cagttgacca aagaaaattt
    14101 catgcaatta gatggcatca aagaaaagtc agctgataaa ttgctcgctg cgattgaggc
    14161 ttctaaatca aattcagctg aaaaactgtt atttggtcta ggaattcgtc atattggctc
    14221 taaagttagt cgcttaattt tagaggtata cggagatatt tctgctcttt taaccgctaa
    14281 agaagaggag atcgcaagga tagatggcct tggctcaacg attgctcaat cacttactca
    14341 atactttgaa caaaaaacgg ctgcgatttt agtagacgag ttaaagacag caggtgtcaa
    14401 catgcattac tcaggtcaaa aagttaatag tgacgcagcg ctttttggtc taactgttgt
    14461 tttaactggc aaattaaatc agctgaaccg aaatgaggcc aaagataaat tagaggcatt
    14521 aggtgccaaa gttactggaa gtgtatctaa gaaaacagat ttagttattg caggtagtga
    14581 cgctggttct aagctagaaa aagcaaagag cttaggaatt cggattgaag atgaagattg
    14641 gctacgccag ctttaagtga ggtaatgtta tgaaaaaaca attgagagca cgtctcattt
    14701 ataatccaac gtcaggacaa gaacttatga gaaaaagtgt tcctgaagtt ttagacatat
    14761 tagaagggtt cgggtatgaa acatctgcct ttcaaacaac agcccaaaag aactctgctc
    14821 taaatgaagc aagaagagcg gcaaaggcag gttttgactt attgattgct gctggaggtg
    14881 atggcactat taacgaagtg gtcaatggaa ttgctccctt gaaaaagcgc cctaaaatgg
    14941 ccattattcc aacaggtacc actaatgatt ttgctagagc gttaaaagta ccaagaggaa
    15001 atccgagcca agcagcaaaa cttatcggaa aaaatcagac tatccagatg gatattggta
    15061 gagctaagaa agatacttat ttcatcaata ttgctgcagc aggaagtttg acggaattaa
    15121 cttacagtgt accaagtcag ttaaaaacga tgtttggata cttggcttat cttgctaaag
    15181 gagtagaact gctcccaaga gttagcaatg tgcctgttaa aattacgcat gacaaaggcg
    15241 tctttgaagg tcaagtttct atgatttttg cagctattac caattctgtt gggggatttg
    15301 aaatgattgc tcctgatgca aaactagatg acggtatgtt tacgctcatt ttaataaaga
    15361 cagcaaatct ttttgaaatt gtacatcttt tgcgtttgat tttggatggt ggtaagcata
    15421 ttacagatcg ccgtgtcgaa tacattaaga ccagtaaaat agtcattgaa ccccaatgtg
    15481 gtaagcgaat gatgattaat ttagatggtg agtacggagg agatgctcca attacattag
    15541 agaacctaaa aaatcatatc actttttttg cagatactga tttgatttct gatgacgctt
    15601 tagtcttaga tcaggacgaa ttggaaattg aggaaatcgt aaagaaattt gctcatgaag
    15661 tagaagactt agaacaagaa ttagaagaat aatacaatag ttattagcta ctgacagttt
    15721 agtcaaatgc actactatta gaatctaaga tttttaaatc aattgaggta gtgttttttg
    15781 atgcactcaa agagtcaatt tattcatatg aggggcctat ttgaggtctc tcttttttga
    15841 tcatatgcaa gaaaatgcca tgttttaggg gtgttattat accctttttg gaatcttgtt
    15901 gcctttttgt tataataaga gtgtaattct atttttaaaa agatcttaac aaagacagac
    15961 tcgtttatca aaaaggttag tattagtcta agaaagcgta ctcagtctaa agaaatctgt
    16021 tgacattctt aattttaagg aatagaatag caaagtaaaa aaaatttaaa ggagtattct
    16081 actatgaatc ctattttcgc attggctttg gcctgttttg gtgtttctct tgccgaaggt
    16141 ttcttgatgg caaatctttt taaagcagca tctcgccaac cagaaatcat tggtcaatta
    16201 cgttcattaa tgatcttggg tgttgccttt atcgaaggta ctttctttgt tacccttgtt
    16261 atggccttca ttcttaaata acagcctatt tgataataga aaaggagggt gttagatgga
    16321 agaagctaaa atacccatgc taaagcttgg tccaataacc tttaatttga ccctacttgc
    16381 tgtttgtatt gtcacaattg ctgttatctt tgcctttgtt ttttgggcaa gtcgccaaat
    16441 gaaattgaaa ccagaaggga agcaaactgc tttagaatat ttaatcagtt ttgtggatgg
    16501 tattggagaa gagcacttag atcataatct acaaaaatct tattcgctgt tactctttac
    16561 catttttctc tttgtggctg tcgctaataa tttagggtta tttactaagt tagagacagt
    16621 taatggctat aacctatgga cctcgccaac agccaatctg gcttttgacc ttgctctatc
    16681 tctttttatt accttaatgg tacacattga aggggttaga cgtcgtggct tggttgccca
    16741 tttgaaacgt ttggctacac cgtggccaat gactccgatg aatttattag aagagttcac
    16801 aaatttctta tcacttgcca ttaggttatt cggtaatatc tttgccgggg aagttgttac
    16861 aggtttgatt gttcaactgg ccaattatcg agtttattgg tggccgatcg ctttcctagt
    16921 caatatggcg tggacagcct tttcagtctt tatttcctgc atacaggctt ttgttttcac
    16981 aaaactaaca gcgacctatc taggaaagaa agttaatgaa tcagaagaat aaggaaggag
    17041 aacaaacgaa tgtcaatcac atttggtgaa ctcgttggga attttattct agtaacaggt
    17101 tctgtaatcg tcttgttact cttgatcaaa aaattcgctt ggggtgcaat tgaatcgatt
    17161 ttacagacac gctcacaaca aatctctcga gatattgatc aggctgagca atcacgtcta
    17221 agtgctcaac agttagaggc aaaaagtcaa gctaacctag atgctagtcg ttcacaagca
    17281 agtaaaatca ttagtgatgc caaagaaatt ggtcaattac aaggtgataa attggtggca
    17341 gaagctactg atgaagcaaa acgcttgaaa gaaaaagcgt tgacagatat tgaacaaagc
    17401 aaatcagacg ctatttcagc agtcaaaaca gaaatgtctg atttaacggt tcttttagcg
    17461 gaaaaaatta tgggagccaa tcttgataag acggcgcaaa gccagcttat tgacagttat
    17521 cttgatgact taggagaagc ttaatgacca aaaaagaaca ggctcttatc gagcaatatg
    17581 caaaaagcct tgttgaggtg gcaagtgaac atcattctct tgatgcctta caagctgatg
    17641 tgcttgcgat tttagagacg tttgtaacaa ctaatcttga tcaaagtcta tcaagtctag
    17701 ctgttccgca tgctgaaaaa ataaaattgc taaccttatt aaagggaaat aattctgttt
    17761 atatgaataa tttcttgaat ctcattttac aaaacgaacg tgaagcttat ttgtatcaaa
    17821 tgcttcaggc tgttttaaat gaaattgcaa tagtttcaaa tcagtatgat gtgacagtta
    17881 cctcatcact acctttgact gaggagcaaa aaagccgtgt tcgagcagtt gttgcaaaga
    17941 aatttgctgt aacagcgggt cgtttaattg aaaaagtaga tccttcgctt attggaggat
    18001 ttattatcag cgtcaataat aaagtaattg ataccagtat tcgtcgtcaa ttgcaagcat
    18061 ttaaaatgaa tttaaaatag aaagtggtgt gacttttggc aattaatgca caagaaatta
    18121 gcgctttaat taaaaagcaa attgagaact tccagccaaa tttcgatgtc acagaaactg
    18181 ggatcgttac ctatattggt gacggtattg cacgtgcccg tggattagat aatgccatga
    18241 gtggtgaact tcttgaattt gaaaatggtg cctacgggat ggctcaaaac cttgagtcaa
    18301 atgatgtggg tattattatt cttggtgatt tttcagcaat ccgtgaagga gatgtggtta
    18361 agcgcaccgg taaaatcatg gaagtaccag ttggtgaagc tctcattgga cgtgtggtaa
    18421 acccacttgg acaaccagtt gatggcttgg gagatattga aacaactggc tttagaccag
    18481 tagaaacgcc agctcctggt gttatgcagc gtaaatccgt ttctgaaccc ttacaaactg
    18541 gattaaaagc aattgatgct ttggttccaa ttggtcgtgg gcaacgtgag ttgattatcg
    18601 gagaccgtca aacaggaaaa acatccgtag ctattgatgc gattttgaac caaaaaggtc
    18661 aagacatgat ttgtatctat gtggctattg gtcaaaaaga atcaaccgtt cgtacacaag
    18721 ttgaaaccct tcgccgttac ggtgccttgg attatactat tgttgtgaca gcgtcagcct
    18781 cacaaccatc accattgctc tttatcgctc cttatgcggg tgttgccatg gctgaagaat
    18841 ttatgtacca aggcaagcat gtcttgattg tctatgatga cttatcaaaa caagcggtgg
    18901 cttatcgtga attgtctctc ttgcttcgtc gtccaccagg tcgtgaggct tacccagggg
    18961 atgttttcta cttgcatagc cgtcttttgg aacgctctgc aaaagtttct gatgatttag
    19021 gtggaggttc tatcacagct ctaccattta tcgaaacaca ggccggagat atctctgcat
    19081 atattgcgac taacgttatt tcgattacag acggacaaat cttcttgcaa gagaacctct
    19141 ttaattcagg aattcgtcca gcgattgatg caggatcatc tgtctcacgt gtaggtggtt
    19201 cagctcaaat taaagcgatg aaaaaggttg ctggtactct tcgtttggat ttggcttcct
    19261 atcgtgaatt agaagccttt acccaatttg gttcggacct tgatgctgca acacaagcaa
    19321 aacttaatcg tggtcgccgt acggttgaaa tcttgaagca accattgcat aaaccgttac
    19381 ctgttgaaaa gcaagtcgtt attctttatg cccttactca tggtttctta gatgatgttc
    19441 ctgtagatga tattttggct tttgaagaag ctctttacga ctactttgac gttcattaca
    19501 atgatttatt tgaaactatc cgtaccacaa aagaccttcc tgaagaagct gcccttgatg
    19561 ccgctattaa agcgtttaaa gaacactcaa actttaagtc atagagggag gatactagta
    19621 tggcaggctc tctaagtgaa attaaagcta aaattatttc aactgagaaa acaagtaaaa
    19681 tcactagtgc catgcgtatg gtttcttctg caaaacttgt taaatctgag caagcagctc
    19741 gtgattttca aatttacgcc tcaaaaattc gccagattac tacagattta ttgaaatcag
    19801 aattaaccat aggatctgat aatccgatgt tggtgtctcg tcctgtcaaa aaaacaggct
    19861 atattgtcat cacgtctgat aaaggacttg ttggaggtta caattcaaaa atcttgaaat
    19921 ctgttatgga tatgattaca gagtaccatg ctgatggtga ctatgaaatc atttctattg
    19981 gtagtgttgg ttcagacttc ttcaaagcaa gagggatgaa tgtggctttt gaacttcgtg
    20041 gacttgcgga tcaaccaagt tttgaacaag tgagacaaat catttcacaa tccgttgaca
    20101 tgtttgtcaa tgagattttt gatgagttat atgtctgtta caatcaccat gtgaatagct
    20161 taaccagcca agtccgtgtt caacagatgt tgccaatttc tgacttggtt gctgatgaag
    20221 cagcagaaga aggtgttact gggtttgaat tagaacctaa tcgtcatgat attttagatc
    20281 aattgttacc tcaattcaca gaaagtttaa tttatggggc tatcattgat gctaaaacgg
    20341 ctgaacatgc agcaggtatg actgccatgc agacagcgac agataacgct aagaatgtca
    20401 ttaacgattt gaccatccaa tataaccgag ctcgtcaagc agcaattaca caagaaatta
    20461 ctgaaattgt tgcaggagca aatgcactag aataaaaaat aacgctgact agcgattagg
    20521 ttattgtcag cattcaattt tatcaatcga gataaaatga cttatgggac ttttctattg
    20581 aaagactgct cttttcagta taaaagaagt tagcaagact aactaagccc tgactatcga
    20641 atataaggag aaatgaatga gctcaggcaa aattgctcag gttgttggtc cagtagtaga
    20701 cgttatgttt gcgagtgggg acaaacttcc agagattaat aatgcattga ttgtttataa
    20761 agatagtgat aaaaagcaaa aaatcgtcct tgaagttgct ctggaacttg gtgacggtat
    20821 ggtgcgaaca atcgctatgg aatcaactga tgggcttaca cgtgggttag aagttcttga
    20881 cactggtcgt gcgattagcg taccagtagg taaagaaact ttgggacgcg tctttaatgt
    20941 acttggagaa accattgatt tggaagaacc atttgcagaa gatgttgacc gtcagccgat
    21001 ccataaaaaa gcaccatcgt ttgatgaatt atcaacatca tcagaaattc ttgaaactgg
    21061 tatcaaggta attgaccttc ttgcccctta ccttaaaggt ggtaaagttg gacttttcgg
    21121 tggtgccgga gttggtaaga ctgtccttat ccaagaatta atccacaata tcgcccaaga
    21181 acacggaggt atttcagtat ttaccggtgt tggtgagcga acacgtgaag gaaatgacct
    21241 ttactgggaa atgaaagaat caggcgttat tgaaaaaacc gccatggttt ttggtcagat
    21301 gaatgagccg cctggggcac gtatgcgtgt agcccttact ggtttaacca ttgctgagta
    21361 tttccgtgat gtagaaggcc aagatgtttt gctctttatt gataatatct tccgtttcac
    21421 tcaggcaggt tcagaagtat cagccctctt aggccgtatg ccttctgctg ttggttacca
    21481 accaacactt gctactgaaa tggggcaatt gcaagaacgg attacatcaa ctcaaaaagg
    21541 ttctgttaca tctatccaag cgatttatgt accagcagat gattatactg accctgcgcc
    21601 agcaacagca tttgctcacc tggattcaac aactaacctt gaacgtaaat tgacacaaat
    21661 ggggatttat ccagccgttg atccattggc ttcaagttca cgggctttat cacctgagat
    21721 tgttggtgaa gagcactatg ctgtggcaac agaagttcaa cgtgtgcttc aacgctaccg
    21781 tgaattacaa gatattattg ctattttggg gatggatgaa ttgtcagatg aggaaaaaac
    21841 attggttgga cgtgcgcgtc gtatccagtt cttcctctca caaaatttta acgttgctga
    21901 acaatttacc ggtcttcctg gttcttacgt gccagttgct gagacagttc gcggctttaa
    21961 agaaattctt gaaggaaaat acgatgatct tccagaagac gcctttcgtt cagttggtcc
    22021 aatcgaggat gtgattaaaa aagccgaaaa aatgggcttt tagtgaggtg atgccatgac
    22081 acaaatgact gttcaagtgg taacgccaga tgggatcaaa tatgatcacc atgctaaatg
    22141 tatttcggta acaacaccag atggggaaat ggggatttta cctaatcaca tcaatttaat
    22201 tgcacctttg caagtccatg aaatgaaaat tcgccgaggt ggtgaagatg aaaaagtgga
    22261 ctggattgct atcaatggtg gtattattga aatcaaagat aatgtggtta ccgttgtcgc
    22321 tgattctgca gaacgtgacc gtgatatcga tgtcagccga gcagagcgag caaaactaag
    22381 agcagaacgt gaaattgcac aggctgaaac gacccacaac attgatgaag ttcgtcgtgc
    22441 caaggttgcc ttgcgccgcg ctttgaatcg tatcaacgtt agtaaaaagt aagtggtgtt
    22501 gataaaagac aaggataagt aagaaaggtt tttaacctat tattttgttt gctgagtctt
    22561 tttaacatca gtttctgaga gtttgaccat actaccttct ctaaacacgt tattattgtt
    22621 gagaggagtt atatggggga actctttttt gtatccacgt tgttagtaga aactcagtaa
    22681 tgacgcttaa tgtattttta ggctatcaac ttagtcagag aggatgtcaa tttaattgat
    22741 aagttggacg gatcaagatg gtatgttata ataatcctat ggaattatta atgaagatca
    22801 ttgccttttc gtgtcattta ttttttattg gccttagtta ccaattattg attagtgtga
    22861 ttgactggac aaaatttagt cattatcatc cagaaaatct tggccggcta cgactttttg
    22921 tctttttatt agctactgcc ctagggtatt tggtgagcca ttgtatgctg gaattgcttc
    22981 aaattagcca gacgctcttt tttgagttct gctaatactg gaataaaaga agtatggtat
    23041 aattttacta cgactaagag atagataagg gagaaaattg tggataaaat aatcattgaa
    23101 ggtggtcaaa cacgcttaga aggagaagtt gttattgagg gtgctaaaaa cgcagttctt
    23161 cctttactgg cagcatctat tttaccttca aaaggaaaaa caattcttag aaacgttcct
    23221 attctatctg atgtttttac catgaataac gtggtacgtg gtttagatat tcgtgttgat
    23281 tttaatgaag cagctaacga aatcacagtt gatgcctcag gacacatttt agatgaagcc
    23341 ccttacgagt acgtgagcca aatgcgtgcc tctattgttg ttttaggacc aattttggca
    23401 cgtaatggcc acgcaaaagt atcaatgcct ggaggctgta cgattggcag taggcctatt
    23461 aatttgcact taaaaggatt agaggctatg ggggcaacga ttactcaaaa aggtggagat
    23521 attaccgctc aagcagatcg actgcaaggt gccatgattt atatggactt cccatctgta
    23581 ggagctaccc aaaacttgat gatggctgcc actttagctg atggtgtgac cactattgaa
    23641 aatgctgctc gtgagcctga aattgttgat ttagctcaat ttttgaataa aatgggagct
    23701 agaattcgtg gtgctggaac ggagacgtta acgataacag gcgtcacaag tcttcatggg
    23761 gtggagcacg atgtggttca agaccgtatt gaagcaggta cttttatggt tgcagcggcg
    23821 atgacttcag gcaatgtttt gattagagat gctgtttggg aacataatcg tccattgatt
    23881 tcaaaattaa tggaaatggg tgtttctgta acagaagaag aatatggtat tcgcgttcaa
    23941 gcaaacacgc caaagctcaa acctgtaacg gttaaaacct tgcctcatcc aggttttcca
    24001 acagacatgc aagctcaatt tacagcttta atggcagtgg tcaatggaga gtctactatg
    24061 gttgaaactg tctttgaaaa tcgatttcaa catctggaag aaatgcgacg catgggctta
    24121 caatctgaaa tccttagaga aactgccatg attcatggtg gaagacaact gcaaggtgct
    24181 cctgtgatgt caacagattt acgtgccagt gctgccctta tcttgacagg gattgttgct
    24241 caaggagtta caatagttaa taatttggtt catttagatc gtggctacta tcaatttcat
    24301 gaaaaattag caaagcttgg agctactatt agtcgtagca gtgaggtcta gcttatgaca
    24361 aatggtttaa aatatgtctt agaacaaatg ttattacttt tagtaattgc tgccttggcg
    24421 tgtctttttt tagccattgg acttatgatt ggttatagtt ttatgggaga tggccatagt
    24481 ccatggcata ttttatctat ggataagtgg gcagagttag tgaataaatt tactggaaag
    24541 taggccttgc ctacttttta tagcatctga gaggaagtat gttaacaagg aaaaaacgaa
    24601 aaagggtgca agagcaaaag gttcctaaaa gagctaatgc cttacttact aagtcaaccc
    24661 ggcaatatca agaccgttca caacttaaga aaggttaccg ttcatttaag ccagcaggtt
    24721 ggcaccgatt gcaccactta aaaggaagtt atgatcacgc ggttgataga gggcatttgc
    24781 taggttatgc tttagttggt ggactgaaag gttttgatgc ttctactggt aatcctgata
    24841 atattgccac tcaactaagt tgggctaatc aagcaaataa accctactcg acaggtcaga
    24901 attactatga aggtttagta cgtcgtgctt tggataaggg gcatcgcgtc cgctaccgtg
    24961 tcaccctact ttatgatggt gataatctac tagccagcgg tagccatttg aaggctaaat
    25021 cctctgatga cagcttggct tttaacgtgt ttgttccaaa tgtccaagca ggattgaccg
    25081 ttgactatcg gacgggacaa atagctatca atctctaagc gaaaagtcac tgatttaaga
    25141 ttgtcactaa gcactttttt tgatagaata gaactaattg aataagtgtc tatgagacta
    25201 gtactaatag agaaattaac agggagtgag ggccgtgact ggaagccttc ttaagggaac
    25261 tattaggaat tcactcagct aaagacaatt aattaaggtc tgataacatc agataaaaac
    25321 ggatggtacc gcgtgtcaac gctccgctta tcaagtggag gagtggcatg cttttttcat
    25381 cgccaaaaga agggaaaact atggatttac aagcacaatt agaggaatta aagtcaaaaa
    25441 cattggagac cttacagtct ttgacgggta atcataccaa agaactgcaa gatttacgcg
    25501 tggctgttct tgggaaaaag ggatctttga cagaattact aaaggggcta aaagacttgt
    25561 ctaatgactt gcgtcctgtg gttggtaaac aggtgaacga agttcgtgac ttattgacaa
    25621 aagcctttga agaacaggcc aaaattgtag aagctgctaa aattcaggcc caacttgatg
    25681 ctgaaagcat tgatgtgact ttaccgggtc gtcaaatgac tctggggcat cgtcatgtgt
    25741 tgacacaaac cagtgaagaa atcgaagata ttttcttagg aatgggtttt cagattgttg
    25801 atggctttga ggtggaaaaa gattattaca actttgagcg catgaatttg ccaaaggatc
    25861 accctgcccg tgacatgcaa gatacatttt atatcacaga agaaatattg ttacgaactc
    25921 ataccagtcc tgtgcaggcc cgtactcttg atcaacatga tttttcaaaa gggcctttaa
    25981 aaatggtttc tccaggtcgt gttttccgtc gtgatactga tgacgctacc cactcacatc
    26041 aattccatca aattgaaggt ttggttgtgg gcaaaaatat ttccatggga gacctcaaag
    26101 ggactcttga aatgatcatc aagaagatgt tcggtgaaga acgtagcatt cgtttgcgcc
    26161 cgtcatattt cccgttcaca gaaccatctg ttgaagtgga tgtttcttgc tttaaatgtg
    26221 gtgggaaagg ctgtaatgtc tgcaagaaaa caggatggat tgaaattctt ggtgctggta
    26281 tggttcaccc aagtgtcttg gaaatgtcag gtgttgatgc aaaagagtat tcaggctttg
    26341 cctttggttt aggtcaagag cgaattgcca tgttgcgtta tggcattaat gacattcgtg
    26401 gtttctatca aggcgaccaa cgtttctctg aacaatttaa ctaaaacgag tcgtttgaag
    26461 acctctgtca gctattttat ctaagattga gtaaaagttg ataggtaatg ataataggtt
    26521 aattgtctaa ggagaaagtc cctctcgctt caacgtcata agggttttga cttggaactt
    26581 aaaatacgaa aggaaatgac tcaaatcacg gcttgagagc tttctctagc cgagtgattt
    26641 gggcactaaa attatgttag tatcttataa atggttaaaa gaattagtag atatcgatgt
    26701 gacaccagta gcacttgctg agaaaatgtc gacaacgggt atcgaggtag aaggaattga
    26761 agtccctgca gaaggtttat caaaactggt tgtggggcat gtgctttctt gtgaagatgt
    26821 gccagagaca catttgcatt tgtgtcaggt agatacaggt gatgaaacgc cacgtcaaat
    26881 tgtgtgtggc gcaccaaatg tgaaagcagg gattaaggtt atcgtggctg tgccaggtgc
    26941 tcgtattgcg gataactata aaatcaaaaa aggcaaaatc cgcggcatgg aatctcttgg
    27001 catgatttgt tccttgcaag aacttggttt gtcagatagt atcattccaa aagaattttc
    27061 agatggtatt caaatcttgc cagaggaggc tgtgccaggt gatgctattt ttaaatacct
    27121 ggacttggac gatcatatca ttgaattgtc cattacacct aaccgtgctg atgcgctttc
    27181 tatgcgaggt gtggcacatg aagtcgcagc tatttatggc aagtcagtat cattccccca
    27241 aaaaaatcta caagaatctg ataaggcaac ttcagaagcc attgaattgt ccattgcctc
    27301 tgataacgtt ctaacttacg ctagccgtgt cgttgaaaat gtcaaagtta agccaagtcc
    27361 acaatggttg caaaaccttt taatgaatgc tggtattcgt cctattaata atgtggttga
    27421 tgtgaccaac tatgtactct tgtattttgg tcaacctatg catgcttttg attatgataa
    27481 atttgaagac cataagattg tggcgcgtgc ggctcgtcaa ggagaaagtt tggtcacact
    27541 tgatggtgaa aaacgggatt tgaccactga ggatcttgtt attaccgtgg ctgagaaacc
    27601 agttgcttta gcaggtgtta tgggtggtca agccactgaa attgatggca actctcagac
    27661 ggttgttctt gaagctgctg tttttgatgg caaatcgatt cgtaaaacca gtggtcgttt
    27721 aaaccttcgt tcggaatcct cttcacgttt tgaaaaaggg gttaactatg caactgtttt
    27781 agaagcgctt gactttgcag cggctatgtt acaagaatta gcagaaggtc aagtcttatc
    27841 aggccatgtt caagctggac aattgccaac agaacctgtt gaagtctcaa ctagccttga
    27901 ctatgtgaat gttcgtttag gaacagaatt aacttttgct gatattcaaa ggatttttga
    27961 ccaacttgga tttggtctaa caggagatga aacaagcttc acggtagctg ttcctcggcg
    28021 tcgttgggat gttagcatcc cagctgactt ggtggaagaa attgcgcgta tctatggtta
    28081 tgataagttg ccgacaacct taccagaggc agggggaacg gctgcagaat taacgccaac
    28141 acaggctctt cgtcgtaaag tccgtggctt agcggaagga ttgggcttga ctgaaatcat
    28201 ttcgtatgcc ttgacaacac cagagaaagc tgttgagttt gcagtggcac caagtcacct
    28261 aacagaactc atgtggccaa tgtctgtgga acgttcagcg cttcgtcaaa atatggtttc
    28321 agggatgtta gatacggtag cttataatgt ggctcgtaaa caaagtaact tggctcttta
    28381 tgaaattgga aaaatctttg aacaagaagc taatccaaaa gaagaccttc ctaatgaagt
    28441 gaaccatttt gcttttgcta tttgtggctt agtggcacaa aaagatttcc aaacccaagc
    28501 tcaggcagtg gatttttacc atgctaaagg tatcttggat actttatttg caaaccttaa
    28561 cctcaaggtt caatatgtgc caaccaaaga cctagcaaac atgcacccag gacggacagc
    28621 gcttatccta ttagatgagc aagtgattgg atttgttggt caagttcatc caggaacagc
    28681 aaaggcttat agtattccag aaacctatgt ggcagaattg gatatggctg ctttagaagc
    28741 agcgttgcca agtgatcaaa cttttgcgga aatcacaaaa ttccctgcta tgacacgtga
    28801 tgttgccttg ttacttgacc gtgaggtgag tcatcaggcc attgtaacag ctattgaatc
    28861 agcaggtgta aaacgcttga ctagcattaa actctttgac gtctatgaag gagcaaccat
    28921 tcaggctggc aaaaaatcaa tggcttatag ccttactttc caaaatccaa atgacaattt
    28981 aacggatgaa gaagttgcca aatacatgga gaaaatcacc aaagcattga cagaacaagt
    29041 cggtgctgaa gtacgctaag aaaaaaactg ggcctctaag gctccgtttt tttctgttat
    29101 aatagaggaa aaaagaactc cagttcaaat gattggagaa aaacatcaga aggtggagga
    29161 aaatcagaat gtatcaaaca aaaataacag gcaaccgcct ttaccacacg gtttcgaaag
    29221 gctacggaga tagtgtaacc ttatttggaa aaactgatca aggggaaacc cctatgagtt
    29281 tactggttat tgccttgtct tcttgtgtga ctatgtgtgt gcagggtttt taccatcgtc
    29341 aatttcagca agacacagtt gctgtttctg ttgaggctag ttatgaggaa gaagtttttg
    29401 aactacttgt taggattgat gatgttttag atgacaaaaa gaaagctgat ttattggctt
    29461 acattggaac ttattgtcgt gtgaaacaac tcctcaaacc tgaagttacg gtgaccatta
    29521 cgttggaagg attgtctcat gtctgatgct agtttgaaaa cttaccaaaa acaatgggct
    29581 tatcaaaagt attgggtcat ggcacactca cagcagcatt acaatgcttt aagagaattg
    29641 tttaaaggaa atcagtggtc agaagaaaag gtgttgactt ttcattgttt gattgaagaa
    29701 gcgcaagcca ttcctcccac agtaaaaagc ttacgaacgg catatcagca tgtttggggg
    29761 tattttaaaa aggtagcgag tcaggaagaa aaagaccatt tcaaagattt ggatgctcag
    29821 ttggaaacta aatctgagga gatgctatgc tttttacaag agatgactgc tcattatcag
    29881 ccctcttatc tgctatcttg ccgtttgatt accaaaggtc cttaaatgct aaacagaaaa
    29941 gtagggattt ctgggaagtt atgctaagct agtgatataa ataaggggga atgagagatg
    30001 tttttagcgc ttaatgaaat gaaacaatcc aaattgcggt atggtttgat tgctggtttg
    30061 ctatgtttgg ttgcctattt gatgttcttt ttgtcaggat tggcctttgg tttgatgcag
    30121 gagaaccgct cggcggttga tctttggaaa gctgacagtg ttttactagc caaggatgct
    30181 gacgctacct tgaccttatc acaggtgtcc agagctcaag aaaatcaaat aacagcagac
    30241 aaggtagctc ctcttgctca actcaatacc gtggcgtggt cagttaaaaa tcctaaggat
    30301 gccgacaaag ttaaggttag acttttcggg attgattcta atagctttat tcgtcctaac
    30361 attgtaaaag gtcgattatt taagactaac aaagaggttg ttttggatca aagccttgca
    30421 aaagaggaag cttttgcgat tggcaaggac ttttacacat cgagttctag tcaagcatta
    30481 actatcgttg gttatactca aaatgctaga tttagtgttg caccagtggt ttatatgaat
    30541 ttggaagctt ttgaaacatt aaaatatgga gaaccactac caaaagataa gcaagttgtt
    30601 aatgctttta tcactaaagg aagtttaaca gattatccta aaaaagactt ccaaaaatta
    30661 gatattaaaa cctttattac taaattacct ggttatagcg ctcaactttt aacttttggc
    30721 tttatgatta gttttcttgt cattatttca gctattatta ttggtatttt tatgtatatt
    30781 ttgactattc aaaaggcacc tatttttggg attatgaaag cgcaaggtat cgggaatata
    30841 acgattgcac aagcagttct gctgcaaacc ttcttgttaa gtgctttagg aagcggttta
    30901 gggttattgg gaacttggtt gacgtcttta gtgctccctg aagcagttcc cttccaaagt
    30961 aattggctag tttacttagc gatattagtc agtatggttt gttttgcttt gttaggaacc
    31021 ttattttctg tttttagcat tgtacgaatt gatcctttga aagcaattgg ataggaggta
    31081 gtgatgtcgg ttttaacatt taaacaagtg accaaaacat ttcaagatgg tcatcacgaa
    31141 atcaacgcat taaaggcaac tgacttttcc attgaagctg gagaatttgt ggctattatt
    31201 ggaccatcag gttctggaaa atcaaccttt ttgaccattg ctggtggctt acagacccca
    31261 agctctggtc agctaatcat tgatggcact gattataccc atttatcaga aaaagagcgc
    31321 tcacgcttgc gcttcaaatc cgttggtttt atcttacaag cttccaatct gatacctttt
    31381 ttaacggtcc agcagcagtt ggaattggtc gatcatttga caggaagcaa agaaaaagcc
    31441 aaagccaatc agttatttga cgatttaggc attgcagggc taaaacatca gttacctcaa
    31501 gaattgtccg gcggagaaag gcaaagagcg gctattgccc gtgctcttta ccatgatcca
    31561 gcactgatat tggcagacga acctactgca agtttggata ctgaaaaagc ctatgaagtg
    31621 gtcaaactgt tggctaagga aagtaaagaa aaaaacaaag ccattattat ggtcactcat
    31681 gatgatcgga tgctaaaata ttgtgacaaa gtctatcgga tgcaagatgg agagttacgt
    31741 caagagagat agtagactgg taagagttta tgatattaga agaaaaaacc aaacaagcac
    31801 agcacttgtc tggttttttt ctaagtgtga ctattatcaa tacgtcacca tttccctaaa
    31861 gcttactata cgggatgcta aaggtatcac catgctcaaa gtcgccatat tggtaacctc
    31921 ggtcaaacca acgttggcgt tgcttagaag ttccgtgggt aaaactatca ggaacggctc
    31981 tgccataggt ttcttcttgc agagtatcat ccccaactgc gtgagctgct gccattgctt
    32041 cctcaatatc tcctttttcc aaaaggcctt gcccttgaac ataattagcc caggcaccag
    32101 catagtaatc agcttgtaat tccaacttga cgttgagctg attggcttta gcctttgatt
    32161 ttccttgtct agtgctagcg taattatcca taattcccaa ttcattttgg atgtggtggc
    32221 caacttcatg agcaatcaca taggccatag cgaaatcacc ttttgcgccg tacttggttg
    32281 atagttcatt ataaaaagaa atatctaaat acactttttg atcgcctggg caatagaaag
    32341 gaccagaaga ggcttgcccc ctgccacaag cggtttgagt tgctccagta tatagaacta
    32401 atgttggttt atgataagtt aaccctttct cacgaaatgt cttagtccaa tagtcctcgg
    32461 tagaggcgaa gaccttactg acaaaagaaa cctgttcttg actagccttg tcaccatttg
    32521 tacgcgttac ttggcttgat tgataggcat tgttgttggt agaagaaggc ttgcctccta
    32581 aaacaccaga taggccacct cctcccataa ctaggagaag gagaagaata accagcttgg
    32641 ttttccaacc tcctcgtgaa aagagtaatt gaagcaggag accgccccct aggccgccgc
    32701 ccccaaaaga accagacgat tgaccacgtc tatcttcaac ctgttgactt tctctcaaat
    32761 catccgtttt cataagtttc tcccatctat ttcttgattt tattataaaa taattctatt
    32821 agaattgcta tttttatgat gagattataa ctaaaaatag gaaaagcaat aggtatttgc
    32881 tataatataa caagggtaga ttcagtttgt ataaaggagt catatgaaat taatttacac
    32941 agagatgagt tactcgatga cagagatttt ggtgaatgaa gcacgaaaag ctgctgacca
    33001 aggttatcga gtgttctata ttgctccaaa ttctctttca tttgaaaagg aaagagaagt
    33061 cttaacctta ttgcctgaac gagggacttt ttcgataata gtcacccggt tcgttcaaat
    33121 gtcaaggtat tttacagttg agagctctcc ttctaagcaa catctagatg acacgacttt
    33181 ggctatgatt ttttatcggg ccttgatgca attaaaacca gaagatttgc cgagttatgg
    33241 tcgtttgcaa aataattcgg tttttattga acaactagtt gagttatata aggaattaaa
    33301 aaacgcacaa ttaagcgtac atgatttaac agggttagac catcctcaaa aacaagaaga
    33361 tcttattaaa attattgagc ttgcagagac gatcatgatt cagcaagatt acaatcaaga
    33421 tagtcctcta caatcttttg caagagcaat taagttagga cttttaaata atcaactatc
    33481 aaaaacagtt attgttattg atggtttttc acgtttttca gctgaagagg attatttatt
    33541 atcactttta aataacaact gtcaagaggt catcattggc agttatgtga gtcaaaaagc
    33601 ttatcaaaaa tcttttatta agggaaatat ctatgaggct agccttcatt ttttgcaaga
    33661 tttggctcaa aaataccata tcaagccggt atttgcgacc tctaatcaag tgtttaaacc
    33721 agccttttca cggctaacac aattatttga agcaactcat gatttttcac aagtagactg
    33781 gcaactccaa aagaatgatc ttgatcactt tagcctttgg caatgtcatc atcaaaaaga
    33841 ggaaattgaa catgtagcaa aatccattcg gcaaaagcta tatgaaggtt accgttataa
    33901 ggatatctta gtgttgctag gagatatgga tgcttatcaa ttacaaattg ggcctatctt
    33961 tgataagttt gaaatcccct actatttagg aaaagcagag cctatggctg cccatccatt
    34021 agtccaattt atagaatcgc tcgaacgaag tcagcgctat aattggcgtc gtgaagacat
    34081 tttaaacatg ttaaaatcag gactttttgg ttgttttgat gatagtgata ttgatcgctt
    34141 tgaggaatat actcagtttg cagatattaa aggatttact aaattttcaa agccttttac
    34201 cattaactct tctaggcagt atcctcttga ctttttaaat gaaatgagac aagacatcgt
    34261 tttacctttg caagaattgt ttaagagtca aaaacagctt ggtgcttcac ttattgacaa
    34321 attaatactt tttttagaaa agattagatt agctgagaat atgcaaggtt tagcacagtc
    34381 ccaattagag gtggaaaaaa acgaagaagt ttggaaaagg tttacagata ttttgacttc
    34441 ttttcatcat atttttggcc aagaaaaatt acggctatca gattgccttg ctttaatcaa
    34501 aacgggaatg aaatcagctc agtatcgtgt tgttccagcg acattagatg ttgtcactat
    34561 taaatcttat gaccttgttc aaccacattc aaaacctttc gtttatgcaa ttggtctaac
    34621 tcaatcacat tttcctaagc aaattcacca ttcaggacta ctttctgatc aagagcgtgc
    34681 tagaattaat gaaataagaa attatcgtca ttttgatatt gctagtgccg aaaatagtaa
    34741 aaaaaaccat caaactgcgt tatctctgtt taatgctgct actaaagagt tagttcttag
    34801 tgttccaacg gtaatcaacg agacattcga tgatctatct ccttatctta aagaattaat
    34861 aaactttggg ttacctcttt tggataaagg caaaaactat ctatcttatg ataatagcga
    34921 tattggtaac tataaagctc tcttgtcaca aattattgct attaaccgac aagatcttat
    34981 agaaatgtca gatcaagata agatgttttg gactgttgtc ttacgttatc tcaggaaaca
    35041 gttaagaaag caacagctag agctacctac ttcagattat cgtttatcaa caaagccttt
    35101 gtctaaagaa gttatcgagg tatgttttcc aaagggaatc cctttaaagc tatctgcaac
    35161 tgctttaacc gttttttata ataatcagta caactatttt ttgaaatatg ttttgaattt
    35221 aaacaaaaca gaatccattc atccagattc cagaattcat ggtcaatact tgcatcgtgt
    35281 ctttgagcgg ttaatgaaag atcacacgca agaaccgttt gataataagt taaaacaagc
    35341 aatctaccat accaatcaag aaagtttttt tcagcaggtt taccaagata atgccgaggc
    35401 tgagtacagt ttagctatac tagaagatat tgttagatca acagcaccta tcttgcaact
    35461 taaccaaaac ataaaggtca ttgatcaaga aaagaacttt catctggata tgggaaatga
    35521 gatattagtc cacgggatca ttgatcgtat tgaccagctg agcgacggta gcttgggcat
    35581 tgttgactat aaatcaagcg cgaatcaatt tgatattggg accttttata atgggttgag
    35641 tccacaacta gtgacttacc tcgcagcctt aaaacagatc gcaccgcacg atataaacca
    35701 attatttggg gctatgtacc ttcatttgca agatcctaaa ttggatctag taacatttaa
    35761 acaaattgat aacacattag tggagagtat ctataaagcg ttgacctata aaggtatttt
    35821 ttcagaagtc gaaaaagagc atctatctac tggtgcttac caaaccaaaa atgctcttta
    35881 ttccaatgac gaattggaaa cacttttaaa ttataacaag tacctctatc ttaaagctgc
    35941 taaacacatt aaaaagggac attttttgat taatccttat acaagcgatg gtaagacagt
    36001 tcaaggagat caattaaaag ccattacccg ttttgaagca gatcttgaca tggcacaggc
    36061 aaggcgacta gtgactctcc ctgctaagga gaaaaaagag tgctttttaa cattaatgag
    36121 aaaggagagc cacttgtgat ttcttttgcc ccatttttaa gccccgaagc tattaaacat
    36181 ttacaagaaa acgaaaggtg cagtgatcag tctcaaaaac gcacagctca acaaattgaa
    36241 gcaatttata ctagtggcca aaatatactt gtatcagctt ctgctggttc aggaaaaacc
    36301 tttgtaatgg tcgaacgcat acttgataaa attttgagag gtgtttcaat tgatcggctt
    36361 tttatctcaa cctttactgt taaagcagct acagaactgc gtgaacggat tgaaaacaaa
    36421 ttatactcac aaattgctca aactacagat tttcaaatga aagtttattt aacagaacaa
    36481 ttgcaatctc ttggtcaagc tgatattggt actatggatg cttttgcaca gaaagtagta
    36541 agtcgctatg gttatagcat tggcatttca tcccaatttc gtatcatgca ggataaagca
    36601 gaacaagatg ttttaaagca agaggtgttt agcaaactct ttagtgagtt tatgaatcaa
    36661 aaagaggcac cggtgtttag ggctcttgtg aaaaattttt ctggtaactg taaagacact
    36721 tcagctttta gagagttagt ttatacttgt tattctttta gccaatcgac agaaaatcca
    36781 aaaatatggt tgcaagaaaa ttttctaagc gctgctaaaa cttaccaaag acttgaagat
    36841 atcccggatc atgatattga actcttactt ttggcaatgc aagacactgc aaatcagcta
    36901 agagatgtga ctgatatgga agattatggg cagctgacta aggcaggtag ccgatctgct
    36961 aaatacacta aacacttaac gatcatagaa aagttgtctg attgggtgcg tgattttaaa
    37021 tgtttgtatg gaaaagccgg attggatcgg ttgatcagag atgtgacagg ccttatacca
    37081 tctgggaatg atgttacagt ctcgaaggta aaataccctg tttttaagac cttgcatcaa
    37141 aaattaaaac aatttaggca tttagaaaca attttaatgt atcagaaaga ctgttttccc
    37201 ttattggaac agttacaaga ttttgtgctt gcgttttcag aagcttattt agctgtcaaa
    37261 atacaagaaa gtgcttttga attttcagat attacacact ttgcaatcaa aattttagag
    37321 gaaaatacgg atattcgcca atcctatcag caacactatc atgaggtgat ggttgatgaa
    37381 tatcaagata acaatcatat gcaagagcga ctcctgacct tactatcgaa cggtcataat
    37441 cgctttatgg taggagatat caaacaatcg atctatcgat ttcggcaagc cgatcctcag
    37501 atttttaatc aaaagtttag agactatcaa aaaaaaactg agcaggggaa agtgatttta
    37561 ctcaaagaaa actttcgtag ccaatcagag gtgttaaatg tcagcaatgc tgtttttagt
    37621 cacttaatgg acgaatcagt aggagacgtc ttatacgatg agcaacatca gttaatagca
    37681 ggtagtcatg ctcaaacagt cccctatcta gaccgtcgtg ctcagttatt gctatataat
    37741 agcgataaag atgatggcaa cgccccttca gatagtgagg gtatttcatt tagtgaggtt
    37801 acaattgttg ccaaagaaat tattaagctt cacaatgata agggtgtccc ttttgaagac
    37861 attacgttac tcgtttcttc aagaacaaga aatgatatca tttctcatac attcaatcaa
    37921 tatggtattc ctatagtaac agatggtggg cagcaaaact atcttaaatc tgttgaagtg
    37981 atggttatgt tagatacatt acgcaccatt aataacccaa gaaatgatta tgcccttgtg
    38041 gctttactgc gctcaccgat gtttgccttt gatgaggatg atttagcaag aatagcactt
    38101 caaaaagaca atgagctaga taaagattgc ctatatgaca agatacaaag ggctgtgatt
    38161 ggaagaggtg ctcatcctga attgattcac gataccttgc ttggcaagtt aaatattttt
    38221 ttaaagacgt tgaaaagttg gcgtcgatac gctaagctag ggtcgttgta tgacttgatt
    38281 tggaaaattt ttaatgatcg tttttatttt gattttgtag ctagtcaagc aaaagcagaa
    38341 caagcacaag ctaatctata cgcattagct ctacgtgcta atcagtttga aaaatcgggc
    38401 tataaagggc tatactgttt tattaaaatg attgataagg tacttgagac gcaaaatgac
    38461 ttagcagatg tggaagtggc tgctcctaaa caagctgtta atttaatgac cattcacaag
    38521 tctaaaggtt tacaatttcc gtatgtattt atccttaatt gtgacaagcg cttctcaatg
    38581 acagatattc ataaatcatt tattctgaat cggcagcacg gtatcggtat caagtacctt
    38641 gcagatatca aaggtttact tggtgaaaca acactcaatt ctgttaaagt aagcatggaa
    38701 accttacctt atcaattgaa caaacaagag ttgcgcttag caactttatc agaacaaatg
    38761 cgcttattgt atgttgctat gacacgagct gaaaaaaaag tttattttat tggtaaagct
    38821 agtaagagca aaagtcaaga tatcacagat cctaaaaagt taggcaaact tttgccgctg
    38881 gctttacgag aacagttatt gacattccaa gattggctat tagcaatagc agatgtattt
    38941 tcaactgaag atctttattt tgatgttcgc tttattgaag atagtgattt gacacaagag
    39001 tcagtcggac gacttcaaac accacagtta ttaaatccag atgatcttaa agataatcgt
    39061 caatcagaaa caattgcacg ggctttagat atgttagaag cagtgtctca attgaatgcc
    39121 aattatgaag cagctattca tttgccaaca gttcgaacgc ctagccaact taaggcagct
    39181 tacgagcctt tattagaacc cattggtgta gatattatag agaaatcttc tcgatcgcta
    39241 tctgatttta ctttgccaca tttttcaaaa aaagtaaaag ttgaagcaag tcatattgga
    39301 tcagctcttc atcagttgat gcaggtgctc cctttgtcaa aaccgataaa tcaacaaacg
    39361 cttttagacg ctttaagaga aattgatagt aacgaagagg taaaaacagc tcttgatctc
    39421 aaaaaaatag agtcgttctt ttgtgataca agcctaggcc aattttttca gacttaccaa
    39481 aaacacttgt atcgagaagc accatttgct attttaaaag ttgaccctat tagtcaagaa
    39541 gaatatgtcc tacgtggtat tatagatgcc tactttttgt ttgatgatca tattgtatta
    39601 gtggactata aaacagataa atacaagcag cccattgagt taaaaaagcg ttaccaacaa
    39661 cagttggagt tatatgcaga agctctcact caaacgtata aacttcctgt gactaagcgc
    39721 tatcttgttt taatgggagg tggaaagcca gaaattgtcg aagtttaagg ttgttgtgac
    39781 acaagggtat tttgcaaatg ataatagtat agttgaggat tttacgaaat gcttgaaaag
    39841 ctattgacct ttgaataata atacaatata ataagtttta ttagtgacaa aaaggaataa
    39901 atatactatg aattttaaaa aaataagatt tggttttttg ctttgcctcc tactttatcc
    39961 tttagcagct tgtaacaagt ctgaacaact taatcactac gaaagaatta aaaaaacaag
    40021 aaaattagtc gttgctgtaa gccctgatta tgctcctttt gaatttaaag ctttagttaa
    40081 tggaaaagat actattgttg gagcagatgt tcaattagct caggctattg ctgatgaatt
    40141 agacgtcgat cttgagcttt caccaatgag ttttgataat gttttgtcta gtttacagac
    40201 tggtaaagct gatcttgcta tttcaggaat ctcacatact aaagaaagag ctaaagtata
    40261 tgatttttca attccttact atcaggcaga aaacgctata gtgatgagag catctgatgc
    40321 taaagtgacc aaaaatatta gtgacctaaa cggtaagaaa gtagccgctc aaaaaggtag
    40381 tatcgaggaa ggtttagtta aaatacaatt accaaaggcc aatctgattt ctttgactgc
    40441 tatgggagaa gccattaatg aactaaaagc aggacaagtc tatgcagtta ccttagaagc
    40501 acctgtagct gctggttttt tagcccaaca taaggatctg gctttagcac cctttagctt
    40561 aaaaaccagt gatggagatg ccaaagcagt ggcccttcct aaaaatagtg gtgacttaac
    40621 caaagccgtt aataaggtta ttgctaagtt agatgagcaa gaacggtaca agtcatttat
    40681 tgcagagaca atagcactaa cgaaaaatac tatgaagtaa taaggttaat aaaaacttta
    40741 ttagtatccg ttggaaaagt gttta
//
LOCUS       DAUDYX010000012        39912 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.012, whole genome shotgun sequence.
ACCESSION   DAUDYX010000012 DAUDYX010000000
VERSION     DAUDYX010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39912
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.012"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            57..344
                     /locus_tag="VHD02_000598"
     CDS             57..344
                     /locus_tag="VHD02_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-damage-inducible protein J"
                     /protein_id="HEQ5497230.1"
                     /translation="MTTVKKNRAVTFQANKELVSEAMTVLNKKNLTLSSALRLFLQNV
                     VVTNEVDLLTEEELEKEKLFKQFQAEINKNIEDVRQGKFYTSEEVRAELGL"
     gene            334..669
                     /locus_tag="VHD02_000599"
     CDS             334..669
                     /locus_tag="VHD02_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEQ5497231.1"
                     /translation="MDYKKYQIIYAPDVLEKLKEIRDYISQNYSSTSGQRKMEQIISD
                     IEKLEVFPEVGFDADEKYGSKISHYHSTKGYTLSKDYIVLYHIEGEENRVAIDYLLPT
                     QSDYIKLFK"
     gene            821..1291
                     /locus_tag="VHD02_000600"
     CDS             821..1291
                     /locus_tag="VHD02_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEQ5497232.1"
                     /translation="MEEFTKPTHKTYSEIFEKWYQAYRDTVEPTTASRTLNLFRLHIL
                     PVMGELPINKTSPLDCQNFITDKAKTFKNIKQIKSYTEKVFDFAIKMKLLKHNPMAEI
                     IMPKRKKTRIENYWTVQELQEFLVIILQKEPYKHYALFRLLAYSGLKERRAMRS"
     gene            1254..1616
                     /locus_tag="VHD02_000601"
     CDS             1254..1616
                     /locus_tag="VHD02_000601"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEQ5497233.1"
                     /translation="MAALRKGELCALKWADIDFQSETLSVNKSLGRLDGQAIKKSTKN
                     DFSVRKIKLDSETISILQEWKAISQKEKTELAVAPLSIESDSIFTYCTREGSIEPLHT
                     DYINNILSKVIKQHSLKK"
     gene            1652..1801
                     /locus_tag="VHD02_000602"
     CDS             1652..1801
                     /locus_tag="VHD02_000602"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497234.1"
                     /translation="MIEIGVDPVNTAKRLGHASSQMTLDTYSHATAVGEDKSIKQFAN
                     CLKNN"
     gene            complement(1921..2313)
                     /gene="rpsI"
                     /locus_tag="VHD02_000603"
     CDS             complement(1921..2313)
                     /gene="rpsI"
                     /locus_tag="VHD02_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEQ5497235.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLIINQPFAVTSTEGSYDVFVNVVGGGYGGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2334..2780)
                     /gene="rplM"
                     /locus_tag="VHD02_000604"
     CDS             complement(2334..2780)
                     /gene="rplM"
                     /locus_tag="VHD02_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEQ5497236.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKVYYTHSMYPGGLKSITAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            complement(2998..3204)
                     /locus_tag="VHD02_000605"
     CDS             complement(2998..3204)
                     /locus_tag="VHD02_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ5497237.1"
                     /translation="MKNLKLKAARAGKDLSQQALADLVGVSRQTIAAVEKGDYNPTIN
                     LCIAICRVLDKTLDDLFWEADDSR"
     gene            complement(3201..3707)
                     /locus_tag="VHD02_000606"
     CDS             complement(3201..3707)
                     /locus_tag="VHD02_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497238.1"
                     /translation="MFDSKQNLAKSQWGFIVITAVMDLVVIVATLLAKTSFQRYLVGG
                     VAFVLTSFLILLVWGLKTAKKLQTRLDSKEVLDSKVRDDQKLPKYDERQKQILLKGYT
                     IGFWFMIVVIWLSLFISRFTEGLVSASFLFTLALWGGLAVQTTYCNLSMGASIFYRHY
                     LDAKEADE"
     gene            complement(3843..4703)
                     /locus_tag="VHD02_000607"
     CDS             complement(3843..4703)
                     /locus_tag="VHD02_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ5497239.1"
                     /translation="MTFTIMTDSTADLNQTWAEDHDIVLIGLTILCDGEVYETVGPNR
                     ISSDYLLKKMKAGSHPQTSQINVGEFEKVFREHARNNKALLYLAFSSVLSGTYQSALM
                     ARDLVREDYPDAVIEIVDTLAAAGGEGYLTILAAEARDSGKNLLETKDIVEAVIPRLR
                     TYFLVDDLFHLMRGGRLSKGSAFLGSLASIKPLLWIDEEGKLVPIAKIRGRQKAIKEM
                     VAQVEKDIADSTVIVSYTSDQGSAEKLREELLAHENISDVLMMPLGPVISAHVGPNTL
                     AVFVIGQNSR"
     gene            complement(4800..5318)
                     /locus_tag="VHD02_000608"
     CDS             complement(4800..5318)
                     /locus_tag="VHD02_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEQ5497240.1"
                     /translation="MKKRILLVDGYNMIAFWQSTRQLFKTNQLDQARNTLLTKLNHYA
                     HFENINIICVFDAQYVPGLRQRYDQYYISVVFTEEDETADSYIERMAAELNTAIHMVE
                     VATSDLNEQWTIFSQGALRVTARELEQRVHTVKADLDKMSRDIDLKTPKLRPFDQGQL
                     IQLKDFMSQLDR"
     gene            complement(5322..6068)
                     /gene="rlmB"
                     /locus_tag="VHD02_000609"
     CDS             complement(5322..6068)
                     /gene="rlmB"
                     /locus_tag="VHD02_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEQ5497241.1"
                     /translation="MEDKDTIETNDIVYGVHAVTESLQANTGNKLYIQEDLRGKKVDN
                     IKSLATQKKVAISWTPKKTLSQMTDGAVHQGFVLRVSAFAYTDVDEILEIAEQEANPL
                     ILILDGLTDPHNLGSILRTADATNVCGVIIPKHRSVGVTPVVSKTSTGAVEHIPIARV
                     TNLSQTLDKLKSRGFWIFGTDMNGTPSDCWNTNGKLALVIGNEGKGISTNIKKQVDEM
                     ITIPMNGHVQSLNASVAAAILMYEVFRNRR"
     gene            complement(6115..6912)
                     /locus_tag="VHD02_000610"
     CDS             complement(6115..6912)
                     /locus_tag="VHD02_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497242.1"
                     /translation="MTTMQKTVSLLSLALLIGLLGTSGKAISVYAQDQHTDNVIAEST
                     ISQVSVEASMRGTEPYIDATVTTDQPVRQPTQATITLKDASDNTINSWVYTMAAQQRR
                     FTAWFDLTGQKSGDYHVTVTVHTQEKAVTGQSGTVHFDQNKARKTPTNMQQKDTSKAT
                     TNSVDVDTKAQTNQSANQEIDSTSNPFRSATNHRSTSLKRSTKNEKLTPTASNSQKNG
                     SNKTKMLVDKEEVKPTSKRGFPWVLLGLVVSLAAGLFIAIQKVSRRK"
     gene            complement(7108..7521)
                     /locus_tag="VHD02_000611"
     CDS             complement(7108..7521)
                     /locus_tag="VHD02_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEQ5497243.1"
                     /translation="MTNPVDVNLINGIALAFEGDAVYSYYVRRHLIFQGKTKPSQLHR
                     LATRYVSAKAQANLIQAMLEAHLLTEKEEDIYKRGRNTNSHTKAKNADIITYRMSTGF
                     EAIMGYLDMMGQKERLEELIRWCIEYVEKQQLISS"
     gene            complement(7514..8857)
                     /locus_tag="VHD02_000612"
     CDS             complement(7514..8857)
                     /locus_tag="VHD02_000612"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEQ5497244.1"
                     /translation="MIKIYDTMTRSLRKFVPLTENTVNMYVCGPTVYNYIHIGNARSA
                     VAFDTIRRYFEYTGYQVNYISNFTDVDDKIIKAATQAGVSPKELSDRFIAAFIEDTKA
                     LGVKPATQNPRVMDYIAEIISFVESLIEKDFAYEADGDVYFRVEKSEHYAKLANKTLS
                     ELEVGASGRTDAETALKENPLDFALWKSAKAGEVSWDSPWGFGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVTVDSEKMSKSLGNF
                     VTVHDMLQTVDGQVLRFFLATQQYRKPINFTEKAIHDAEINLKYLKNTLQQPLTETAD
                     EQELKQFVIAFQDAMDDDFNTANGITVVFDMAKWINSGSYTEPVKSAFEKMLAVFGII
                     FEEEVLEVDIEALIAKRQEARANRDFATADAIRDQLAAQGIKLLDTKDGVRWLRD"
     gene            complement(8885..9115)
                     /locus_tag="VHD02_000613"
     CDS             complement(8885..9115)
                     /locus_tag="VHD02_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497245.1"
                     /translation="MNKIRVGLYVIMSLSILVFSITLVASLIFLLRQDMVKAIIYFIL
                     TLFWLIGLVINIKLLKRWNSISEKDLGYQDDN"
     gene            complement(9594..10175)
                     /gene="cysE"
                     /locus_tag="VHD02_000614"
     CDS             complement(9594..10175)
                     /gene="cysE"
                     /locus_tag="VHD02_000614"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEQ5497246.1"
                     /translation="MGWWKESIAIVKALDPAARNSLEVILTYPGIKALAAHRLSHFLW
                     RHHFKLLARMHSQFWRFWTQIEIHPGAQIAPGVFIDHGAGLVIGETAIVEKGVMLYHG
                     VTLGGTGKDCGKRHPTIRQGALISAHAQVIGPIDIGANAKVGAAAVVLSDVPEDVTVV
                     GVPAKIVRVHGQKDNRQIQSLQKQREVSYQLSK"
     gene            complement(10184..10936)
                     /locus_tag="VHD02_000615"
     CDS             complement(10184..10936)
                     /locus_tag="VHD02_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEQ5497247.1"
                     /translation="MTKSNELDIRLRAFINAPDNFLDSLALVNAFHNFPVWAAKEPYV
                     IEVEGVKVTPVFTDKEDMARFKEEQKSAQSQYWLERSALAVLEEVITSGAAGLVFNLK
                     KKGDFGNSTIFKSSDMIQFMNHYTTVLNTLMSDDNVAADTMEKVYLVPAFVYPKDNNH
                     YDRLFPTMSTPEGKSYVPAFSNLQSFAKWYNQDDFGGLFRKAEGVILTWTIDDIYQPR
                     NGENELDETFGVAINPFDDQQILVDWSELDKS"
     gene            complement(10929..13061)
                     /gene="pnp"
                     /locus_tag="VHD02_000616"
     CDS             complement(10929..13061)
                     /gene="pnp"
                     /locus_tag="VHD02_000616"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEQ5497248.1"
                     /translation="MSKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGDSTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFI
                     INPDKEQMEASLLELTVAGSKEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQE
                     QIVAVVGKEKAEVELLQVDADLQADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVK
                     AEYEERYAEDENLATIMRDVAEILEQMEHAEVRRLITEDKIRPDGRKIDEIRPLDAVV
                     DFLPKVHGSGLFTRGQTQALSILTLAPMGETQIIDGLAPEYKKRFLHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIAGITPQILEEALAQAKKARFEILDVIEATIAEPRPELAPTAPKIDTIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSIYSSDQAAINRTKEIIAGLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTTNVSDVLEVGEDVDVKVIKI
                     DEKGRVDASMKALIPRPPKPEKKEEKHD"
     gene            complement(13342..14073)
                     /locus_tag="VHD02_000617"
     CDS             complement(13342..14073)
                     /locus_tag="VHD02_000617"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEQ5497249.1"
                     /translation="MMTKKLNVKVFSDGAVLETMLKDLQTGLVTGFTTNPSLMKKAGI
                     SSYIGFAKEVLAKITDYPVSFEVFADDLASMEKEAEKIASLGDNVYVKIPVTTSTGES
                     TCSVIQKLSAKGIKLNVTAIFTIEQTQAVVDHLTAGVPAIVSVFAGRIADTGVDPMPI
                     MEEALRICLQKEGVELLWASPRETYNIYQADQLGVDIITCTTDLIAKLPLQGKDLEDY
                     SLETVQMFLKDSTSLGFKILEDDNH"
     gene            complement(14083..15462)
                     /locus_tag="VHD02_000618"
     CDS             complement(14083..15462)
                     /locus_tag="VHD02_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEQ5497250.1"
                     /translation="MEALLSFIRDILKEPAFLMGLIAFAGLVALKTPAHKVLTGTLGP
                     ILGYLMLAAGAGVIVTNLDPLAKLIEHGFSITGVVPNNEAVTSVAQKILGVETMSILV
                     VGLLLNLTFARFTRFKYIFLTGHHSFFMACLLSAVLGAVGFKGSLLIILGGFLLGAWS
                     AISPAIGQQYTLKVTDGDEIAMGHFGSLGYYLSAWVGSKVGKDSKDTEDLQISEKWSF
                     LRNTTISTGLIMVIFYLVATVASVLRNASVTEELAAGQNPFIFAIKSGLTFAVGVAIV
                     YAGVRMILADLIPAFQGIANKLIPNAIPAVDCAVFFPYAPTAVIIGFASSFVGGLLGM
                     LILGVAGGVLIIPGMVPHFFCGATAGIFGNSTGGRRGAMIGAFANGLLLAFLPAMLLP
                     VLGKLGFSNTTFGDVDFGVLGILLGRLGTSIGQIGIYLVVAVLAIVLVLPNFLSKSQE
                     AINNVSEEI"
     gene            complement(15489..15773)
                     /locus_tag="VHD02_000619"
     CDS             complement(15489..15773)
                     /locus_tag="VHD02_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ5497251.1"
                     /translation="MIKIVTVCGNGIGSSLLLRMKVEAIASSLGIDVDAESCDSNAAV
                     GKGADLFVTVKEFKDIFPEDAKVCIVKSYTNRKKIEEDLVPVLKEMSGKE"
     gene            complement(15766..17826)
                     /locus_tag="VHD02_000620"
     CDS             complement(15766..17826)
                     /locus_tag="VHD02_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEQ5497252.1"
                     /translation="MLSHELIRNYQLFSKYKGHSLEAFESILKTSKRHILADIAKIND
                     TLSLYQLPLIALDRQLVYPPDLTEKDLLNRMLPTLDDYLFQDERLDMIIIYIMMAKEF
                     ISINHLESLLRLSRNSVIADLNLVRDRVQAFQVTLAYNRQDGYFFEGEPLALRRLLES
                     AVSSLLQVTSGPWVFSYLLHELGLPDQKKVMAATLEELSRENHLTFISEKLRDLIYFF
                     CLLAHRPFSRDVRGEAVDTFPLASPAVETMVDQLLVNFPSLTEEKYLVQSRLLGCIQG
                     DLDLVFQQPIYDIMEEIINSVAVNTGLSITDTPELRQNLYSHLLPAYYRLYYDINLTN
                     PLKEQIKQDYESLFYLVKRSLSPLEKQLGKSVNEDEVAYFTIHFGRWLQAPKKRPSNQ
                     LVALSVCPNGISSSLMLEATLKELFPQLQFIRIHQLDKIKLLDPASFDLIFSTVAFDC
                     AKPVYVTQALMGPVEKMMLKKMVCDDFHLPLSEQFALDDLLSIIHKHTTITNKEGLVS
                     DLSRYLIGNHLTIEKGGLGLLDLLTADFIRQADAVSDWQEAIRLAAQPLLEHQMIETS
                     YIDGMIDSVNELGAYIVLAPKVAVPHAAPEKGTRQLGMSLLQLKEPVSFDLKQEGDPD
                     KQVQLIFVLSAVDSSSHLKALQELSLILDDDEHIEQLIEAKNTEEIMSLISHMIEKGD
                     ESHD"
     gene            complement(18178..18447)
                     /gene="rpsO"
                     /locus_tag="VHD02_000621"
     CDS             complement(18178..18447)
                     /gene="rpsO"
                     /locus_tag="VHD02_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEQ5497253.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNSHIK
                     EHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            complement(18623..18823)
                     /locus_tag="VHD02_000622"
     CDS             complement(18623..18823)
                     /locus_tag="VHD02_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497254.1"
                     /translation="MTMLGYRLSAEVLGSAILPLLAGYLIRQAIAQVYSDLKTASWLF
                     GLASSVAYGGLVKAKRAHSTTR"
     gene            18949..19206
                     /locus_tag="VHD02_000623"
     CDS             18949..19206
                     /locus_tag="VHD02_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ5497255.1"
                     /translation="MTQNVCQQNITYTVKERFASYTLEAQANQEVHLNLTLLANRFGT
                     SDRHLKHVLKQPIFQRIIERICFKTCRIADLDALTQLSVNN"
     gene            19333..19947
                     /locus_tag="VHD02_000624"
     CDS             19333..19947
                     /locus_tag="VHD02_000624"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEQ5497256.1"
                     /translation="MSAQDKLIKPSHLITMDDIIREGNPTLRAVAKEVSLPLCDEDIL
                     LGEKMMQFLKHSQDPVMAEKLGLRAGVGLAAPQIDVSKRIIAVLVPNLPDKEGNPPKE
                     AYSWQEVLYNPKIVSHSVQDAALSDGEGCLSVDRVVEGYVVRHARVTVDYYDKEGQQH
                     RIKLKGYNAIVVQHEIDHINGVLFYDRINAKNPFETKEELLILD"
     gene            complement(19980..20522)
                     /locus_tag="VHD02_000625"
     CDS             complement(19980..20522)
                     /locus_tag="VHD02_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEQ5497257.1"
                     /translation="MKHILFIVGSLREGSFNHQLAAQAQKALEHQAVVSYLNWKDVPV
                     LNQDIEANAPLPVVDARQAVQSADAIWIFTPVYNFSIPGSVKNLLDWLSRALDLSDPT
                     GPSAIGGKVVTVSSVANGGHDQVFDQFKALLPFIRTSVAGEFTKATVNPDAWGTGRLE
                     ISKEIKANLLSQAEALLAAI"
     gene            complement(20654..21082)
                     /locus_tag="VHD02_000626"
     CDS             complement(20654..21082)
                     /locus_tag="VHD02_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEQ5497258.1"
                     /translation="MSHLDKNTALKAMVVFRKAQRTLDAFGADIFKKADLTATQFSVL
                     EVLYTKGCMRINHLIDSLLATSGNMTVVLNNMERNGWISKCKDKTDKRAYVVTLTDKG
                     NRLIEAVLPKHVARVEEAFAVLTEKEQLCLIELLKKFKQL"
     gene            complement(21192..25589)
                     /locus_tag="VHD02_000627"
     CDS             complement(21192..25589)
                     /locus_tag="VHD02_000627"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEQ5497259.1"
                     /translation="MSDLFAKLMDQIEMPLDMRRSSAFSSADIIEVKVHSVSRLWEFH
                     FAFAAVLPIATYRELHDRLIRTFEAADIKVTFDIQAAQVDYSDDLLQAYYQEAFEHAP
                     CNSASFKSSFSKLKVTYEDDKLIIAAPGFVNNDHFRNNHLPNLVKQLEAFGFGTLTID
                     MVSDQEMTEHLTKDFVSSRQALVKKAVQDNLEAQKSLEAMMPPVEEATPAPKFDYKER
                     AAKRQAGFEKATITPMIEIETEENRIVFEGMVFDVERKTTRTGRHIINFKMTDYTSSF
                     ALQKWAKDDEELRKFDMIAKGAWLRVQGNIETNPFTKSLTMNVQQVKEIVHHDRKDLM
                     PEGQKRVELHAHTNMSTMDALPTVESLIDTAAKWGHKAVAITDHANVQSFPHGYHRAR
                     KAGIKAIFGLEANIVEDKVPISYDPVDMDLHEATYVVFDVETTGLSAMNNDLIQIAAS
                     KMFKGNIVEQFDEFIDPGHPLSAFTTELTGITDKHLQGAKPLVTVLKAFQDFCKDSIL
                     VAHNASFDVGFMNANYERHDLPKITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQVSL
                     DHHHMANYDAEATGRLLFIFLRDAREKHGIKNLLQLNTDLVAEDSYKKARIKHATIYV
                     QNQVGLKNMFKLVSLSNIKYFEGVPRIPRTVLDAHREGLLLGTACSDGEVFDAVLTKG
                     IDAAVDLAKYYDFIEIMPPAIYQPLVVRELIKDQAGIEQVIRDLIEVGKRAKKPVLAT
                     GNVHYLEPEEEIYREIIVRSLGQGAMINRTIGRGEGAQPAPLPKAHFRTTNEMLDEFA
                     FLGKDLAYQVVVQNTQDFADRIEEVEVVKGDLYTPYIDKAEETVAELTYQKAFEIYGN
                     PLPDIIDLRIEKELTSILGNGFAVIYLASQMLVNRSNERGYLVGSRGSVGSSFVATMI
                     GITEVNPMPPHYVCPSCQHSEFITDGSVGSGYDLPNKPCPKCGTPYQKDGQDIPFETF
                     LGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGDEYAFRAGTVGTVAEKTAYGFVKGYE
                     RDYGKFYRDAEVDRLAAGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAS
                     WQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPITIPADDPGVMALFSGTE
                     ILGVTPEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWLGNA
                     QDLIKEGIATLKTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGY
                     IDAMRENNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHHPIMYYCAYFSIR
                     AKAFELKTMSGGLDAVKARMEDITIKRKNNEATNVENDLFTTLEIVNEMLERGFKFGK
                     LDLYKSDAIEFQIKGDTLIPPFIALEGLGENVAKQIVKARQEGEFLSKMELRKRGGAS
                     STLVEKMDEMSILGNMPEDNQLSLFDDFF"
     gene            complement(25843..27699)
                     /locus_tag="VHD02_000628"
     CDS             complement(25843..27699)
                     /locus_tag="VHD02_000628"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEQ5497260.1"
                     /translation="MKQSKLLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMREEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDNS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHHNYEDLDVTYEDYRQAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAITPART
                     DLDRWVVLDKSIASMDDIPKEVLEEIKAELAAWMISGEDTIAYSTESSYAANLEMATN
                     EYKPSSKVAAEDALAEVETPHCKTIDEVAAFLSVDETQTIKTLLFVADNEPVVALLVG
                     NDHINTVKLKNYLAADFLEPASEEEARAFFGAGFGSLGPVNLAQGSRIVADRKVQNLT
                     NAVAGANKDGFHVTGVNPGRDFQAEYVDIREVKEGEMSPDGHGVLQFARGIEVGHIFK
                     LGTRYSDSMGATILDENGRAVPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGDYRY
                     AWGVNFPKELAPFDVHLITVNVKDQVAQDLTAKLEADLTAKGYDVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKAAEGIVEIKIKATGASIEVNAENLIETLEILTKEH"
     gene            complement(27897..29156)
                     /gene="rseP"
                     /locus_tag="VHD02_000629"
     CDS             complement(27897..29156)
                     /gene="rseP"
                     /locus_tag="VHD02_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEQ5497261.1"
                     /translation="MLGIITFIIIFGILVIVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHVDQGGTLYTLRMLPLGGYVRMAGWGDDKTEIKTGTPASLTLNEQGFVKRINLSQS
                     KLDPTSLPMHVTGYDLEDQLSITGLVLEETKTYKVAHDATIVEEDGTEIRIAPLDVQY
                     QNASIGGRLITNFAGPMNNFILGIVVFILLVFLQGGMPDFSSNHVRVQENGAAAKAGL
                     RDNDQIVAINGYKVTSWNDLTEAVDLATRDLGPSQTIKVTYKSHQRLKTVAVKPQKHA
                     KTYTIGVKASLKTGFKDKLLGGLELAWSGAFTILNALKGLIIGFSLNKLGGPVAMYDM
                     SNQAAQNGLESVLSLMAMLSINLGIFNLIPIPALDGGKILMNIIEAIRRKPIKQETEA
                     YITLAGVAIMVVLMIAVTWNDIMRVFF"
     gene            complement(29229..30023)
                     /locus_tag="VHD02_000630"
     CDS             complement(29229..30023)
                     /locus_tag="VHD02_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEQ5497262.1"
                     /translation="MKERVVWGGVAVAIFLPFLIIGNLPFQLFVGVLAMIGVSELLKM
                     KRLEVFSFEGVFAMLAAFVLAVPMDHYLTFLPIDANVAFYSLMVFFILAGTVLNSRAY
                     SFDDAAFPIATSFYVGIGFQHLINARLSGIDKVFLALFIVWATDIGAYLIGRQFGRRK
                     LLPTVSPNKTIEGSLGGIACAVLVSFIFMVIDRSVYAPHHFLTMLVLVALFSIFAQFG
                     DLVESALKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHLFGLF"
     gene            complement(30036..30785)
                     /locus_tag="VHD02_000631"
     CDS             complement(30036..30785)
                     /locus_tag="VHD02_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEQ5497263.1"
                     /translation="MFGLKAKSTKKVLGSIPKHIGIIMDGNGRWAKKRLKPRVFGHKA
                     GMDALQEVTITASELGVKVLTVYAFSTENWSRPQDEVSFIMNLPVTFFDKYVPVLHEN
                     NVKIQMIGETSRLPEDTLAALNAAIDKTKRNTGLILNFALNYGGRAEITSAVRFIAQD
                     VLDAKLNPGDITEDLIANYLMTDHLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEFY
                     FTPVLWPDFKKAELLKAIADYNHRQRRFGKV"
     gene            complement(31004..31369)
                     /gene="yajC"
                     /locus_tag="VHD02_000632"
     CDS             complement(31004..31369)
                     /gene="yajC"
                     /locus_tag="VHD02_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEQ5497264.1"
                     /translation="MGFPTILMFVVMLGLIWFMQRQQKKQAQERQNQLNAIEKGDEVV
                     TIGGMFAIVDEVDTTAKKIVLDVDGVFLTFELSAIKRIVTKATTETTLVETEETSVVN
                     EPAVETVDSTDTDSAIESH"
     gene            complement(31485..31859)
                     /locus_tag="VHD02_000633"
     CDS             complement(31485..31859)
                     /locus_tag="VHD02_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEQ5497265.1"
                     /translation="MRPKEKETSMTFEEIVANFIPSSIAEVTSAIASGKDMIVFLGRS
                     SCPYCRRFAPKLAQVATDNQKEVYFVDSENAADAAELAAFRENYQLVTVPALLVSYDQ
                     HQRAVCDSSLTPDDILAFLTRE"
     gene            complement(31974..35471)
                     /locus_tag="VHD02_000634"
     CDS             complement(31974..35471)
                     /locus_tag="VHD02_000634"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEQ5497266.1"
                     /translation="MKKKVNQGSKRYQYLLKKWGIGFVIAATGTVMLGCTPSILTHQV
                     AAKTIVGLARDEAQQGDGNAKSGDGFQSSSKEAKPVLDSSSANPASIAEHHLRMHFKT
                     LPAGESLGSLGLWVWGDVDQPSKDWPNGAITMTKAKKDDYGYYLDVPLAAKHRQQVSY
                     LINNKAGEHLSKDQHISLLTPKMNEVWIDENYHAHAYRPLKKGYLRINYHNQSGHYDN
                     LAVWTFKDVKTPTTDWPNGLDLSHKGPYGAYVDVPLKEGANEIGFLILDKSKTGDAIK
                     VQPKDYLFKELDNHTQVFVKDTDPKVYNNPYYIDQVSLKGAEQTTPNEIKAIFTTLDG
                     LDEDAVKQNIKITDKAGKTVAIDELTLDKDTSVMTLKGDFKAQGAVYTVTFGEVSQVA
                     RQSWQLKDKLYAYDGELGATLAKDGSVDLALWSPSADSVKVVVYDKQDQTKVLGQADL
                     TKSDKGVWRAHLTSDSVKGISDYTGYYYLYEITRGQEKVMVLDPYAKSLAAWNDATAT
                     DDIKTAKAAFIDPSKLGPTGLDFAKINNFKKREDAIIYEAHVRDFTSDKALEGKLTHP
                     YGTFSAFVEQLDYLKDLGVTHVQLLPVLSYFYANELDKSRSTAYTSSDNNYNWGYDPQ
                     HYFALSGMYSANPNDPALRIAELKNLVNEIHKRGMGVIFDVVYNHTARTYLFEDLEPN
                     YYHFMNADGTARESFGGGRLGTTHAMSRRILVDSITYLTREFKVDGFRFDMMGDHDAA
                     AIEQAFKAAKAINPNTIMIGEGWRTYQGDEGKKEIAADQDWMKATNTVGVFSDDIRNT
                     LKSGFPNEGTAAFITGGAKNLEGLFKTIKAQPSNFEADAPGDVVQYIAAHDNLTLHDV
                     IAKSINKDPKVAEEEIHKRIRLGNTMILTAQGTAFIHSGQEYGRTKQLLNPDYKTKVS
                     DDKVPNKATLIDAVAKYPYFIHDSYDSSDAVNHFDWAKATDSIAHPISNQTKAYTQGL
                     IALRRSTDAFTKATKAEVDRDVTLITQAGQDGIQQEDLIMGYQTVASNGDRYAVFVNA
                     DNKTRKVVLPQAYRYLLGAQVLADAEQAGVTAIAKPKGVQFTKEGLTIDGLTALVLKV
                     SSKTANPSQQKSQTGNHQTKTPDGSKDLDKSLMTRPKRAKTNQKLPKTGEASSKGLLA
                     AGVALLLLAISLLMKRQKD"
     gene            complement(35669..37282)
                     /locus_tag="VHD02_000635"
     CDS             complement(35669..37282)
                     /locus_tag="VHD02_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEQ5497267.1"
                     /translation="MKKHWWHKATIYQIYPRSFKDTNGNGIGDLKGITSQLDYLQKLG
                     ITAIWLSPVYQSPMDDNGYDISDYEAIADVFGDMADMDELLAAANERGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDNASGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIAKGIGGFRMDVIDLIGKVPDLEITGNGPRLHDYL
                     KEMNQATFGNHDVMTVGETWGATPEIARQYSRPENKELSMVFQFEHVGLQHKPDAPKW
                     DYAKELDVPALKAIFSKWQTELKLGEGWNSLFWNNHDLPRVLSIWGNDSTYREKSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFKDLTEVNDIESLNYAKEAMGNGVSAARVMDS
                     IRKVGRDNARTPMQWSKDTHAGFSKAQETWLPVNPNYQDINVADALADPDSIFYTYQQ
                     LIALRKEQDWLVEADYHLLPTADKVFAYQRQLGEETYVIVVNVSDQEQVFAKDLAGAE
                     VVITNTDVDKVLETKHLQPWDAFCLKLKA"
     gene            complement(37411..38544)
                     /gene="ugpC"
                     /locus_tag="VHD02_000636"
     CDS             complement(37411..38544)
                     /gene="ugpC"
                     /locus_tag="VHD02_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEQ5497268.1"
                     /translation="MVELNLNHIYKKYPNTTHYAVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIGGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYKKDDIDRRVKEAAQILGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPQGN
                     GTIGKIEQVGSPQELYSLPANKFVAGFIGSPAMNFFEVEVKDGRIVSEDGLAIAIPEG
                     QAKMLEAAGYKGKKVTFGIRPEDISSRQIVHDTYPSATVTAEVLVSELLGSETMLYVK
                     LGQTEFASRVDARDFHSPGEQVSLTFNVAKGHFFDRDTEQAIR"
     gene            complement(38842..39690)
                     /locus_tag="VHD02_000637"
     CDS             complement(38842..39690)
                     /locus_tag="VHD02_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEQ5497269.1"
                     /translation="MELKDYFPEMQVGPHPLGDKEWVSVKEGDQYVHFPKSCLSEKER
                     LLLEVGLDQCEVLQPLGSPWQRYLLDHQGNPPQLFETSQFIYLNHQQALPADLVELLQ
                     QMIAGLEVILPISATQTAFLCRQAVSIKVLRWLEDLLPTLESDFGLALTMFVGNAWYQ
                     VAAGTLRECFEEECQLLTAYLRQQSGRKLLTFSGLMLWSLLSHHTFLALTRQFQQFLS
                     PQSDMADVVHALWSEHGNLVQTAQRLYIHRNSLQYKLDKFAQQSGLHLKQLDDLAFAH
                     LFLLKY"
BASE COUNT        11759 a         8873 c         7522 g        11758 t
ORIGIN      
        1 tgactttttt gtatatatga tataattaaa taaattacat aaaggggtta cataaaatga
       61 ctacagtaaa aaaaaacaga gcggttacct ttcaagctaa taaagaattg gtaagcgaag
      121 caatgacagt attaaacaaa aaaaatttaa ccttatcatc tgctttaaga ttattcctac
      181 aaaatgtcgt tgtcacaaac gaggttgact tattgacgga agaggagtta gagaaagaaa
      241 aacttttcaa gcaatttcaa gctgaaatca acaaaaatat tgaggacgtt cgtcaaggga
      301 agttttatac ctctgaagaa gtgagggctg aacttggact ataagaaata tcagattatc
      361 tatgctcctg atgttttaga gaagctaaaa gaaattcgtg attatatttc tcaaaactac
      421 tcctcaacat caggtcagcg taaaatggag caaatcatta gcgacataga aaaactcgag
      481 gttttcccag aagttggttt tgatgccgat gaaaaatatg gttcaaaaat cagccattat
      541 cacagcacta aaggttacac cttaagtaaa gattatattg tcctatacca tatcgagggg
      601 gaagaaaata gggtcgctat tgattacttg cttcctactc aaagcgacta tataaagtta
      661 ttcaaataac acgttaaaag ccttagcagt cttgctaggg cttttttggt agattaccaa
      721 ttaactagga gcaaaatgag ttcgggctgt ttaatagata ttccacaaag aagccaaaga
      781 ctacgaagct aggctaatct accttaaggc ttcaggaaac ttggaggagt tcaccaagcc
      841 aactcataaa acttatagcg agatattcga gaaatggtat caagcttatc gagatacggt
      901 agagccaacc acggcttctc gtactcttaa tctgtttaga cttcacattt tgccagtcat
      961 gggagaattg cccattaaca aaacctcccc tttagactgc cagaacttca tcaccgataa
     1021 agcaaagacc tttaagaata tcaagcaaat caagtcctac actgaaaagg tctttgattt
     1081 tgccatcaag atgaaactgc tcaagcataa ccctatggct gaaatcatta tgccaaagcg
     1141 gaaaaagaca cgaatagaaa actattggac ggttcaagaa cttcaggagt ttttagtcat
     1201 aatacttcag aaagagcctt acaagcacta cgcactattt cggctactag cttatagcgg
     1261 ccttaaggaa aggcgagcta tgcgctctta aatgggctga tatagacttt cagagtgaga
     1321 cactaagcgt caataagagc ctaggacgat tagacggaca ggcaatcaaa aaaagcacta
     1381 aaaatgattt ctcagtaaga aagattaagc tagacagcga aactatttct attctgcaag
     1441 aatggaaagc catcagccaa aaagaaaaga cagaactagc agttgcacct ctttcaattg
     1501 agtctgactc tatatttaca tactgtacta gagaagggag tattgaacca cttcatacag
     1561 actatattaa caatatctta tcaaaggtca ttaagcaaca tagtcttaaa aaataagtcc
     1621 tcacggcttc aggcataccc atgcaaccct gatgattgag attggagttg acccagtaaa
     1681 tacagccaaa aggttaggac atgctagtag tcaaatgact cttgatacat acagtcatgc
     1741 tactgcagtc ggagaagaca aatcaatcaa acaatttgct aactgtctca aaaataacta
     1801 aataaacgta ttaaaccaag tcaaaagttt tccgaaacgg tatcaaaagc aaaaaacgac
     1861 aaaaaaagca ccttgcaaga gacttgcaaa atgcttgtaa tcgttgatgt aaagctgttc
     1921 ttaacgtttt gagaattgtg aagctttacg agctttctta agacctggtt ttttacgttc
     1981 aaccatacgt gcgtcacgtg taagaaggcc agcgcgtttc aatgaatcgc ggaagtctgg
     2041 gtctacttga agaagcgcac gagcgatacc atgacggatc gcacctgatt gtccaccgta
     2101 accaccacca acaacgttaa cgaaaacgtc gtatgaacct tcagttgatg taactgcaaa
     2161 aggttggttg atgatcaaac gtaagtcagc atgtgggatg tattcttcta catctttttt
     2221 gttaacagtg attttaccag tacctggaac caaacgaacg cgtgcaacag cgtttttacg
     2281 acggccagta cctgcatatt gtgcttgtgc cattatttaa tgctcctttc ctcttagata
     2341 agtcctgaga tgtcaagtac ttctggttgt tgtgctgcgt gagtatgctc accgccaacg
     2401 aagactttca atttcatacc ttgtgcacgt ccaagagtgt tatgtggaag catgccttta
     2461 actgattttt caatcaagcg aactgcgttt ttagagcgaa gttcaccagc agtgattgat
     2521 ttcaaaccac ctgggtacat tgagtgagtg tagtaaactt tatcagttgc ttttttacca
     2581 gttaatttaa ctttttcagc gttgataacg ataacaaaat cacctgtatc agtatgtggt
     2641 gtgaaagttg gtttgttttt tccgcgaagt acgctagcaa ctactgcaga aagacgtcca
     2701 agtggcacat cagttgcgtc aacaacgtac catttgcgtt caacttggcc tggtttagcc
     2761 atgaaagttg ttttgttcat gatttctcct atacgaattc gtaatatttg tttacaaggt
     2821 gatgggtgtt ccgtcccatc gaaggtattt ggaaggttcc ggggcctttc aaatggggta
     2881 aacaataccg cctactatga tatcaaactt aagaggctcc agtcaagtgc tttagctgat
     2941 tttattggat tattttttct ttcgatttag gagaatggca gagctgctct gctggtgtta
     3001 acgagaatca tcagcttccc aaaaaaggtc atctaaagtc ttatcaagca ctcgacaaat
     3061 ggcaatgcaa agattaatcg ttgggttgta atcacctttt tcaacagccg caatggtttg
     3121 tctggagacg cctaccaaat ctgctagagc ttgttgagac aaatctttgc ctgcacgcgc
     3181 tgcttttaat ttgaggttct tcattcatct gcctctttag catccagata atgacgatag
     3241 aaaatactag ctcccatact aaggttacaa tatgtcgttt gaactgccaa ccctccccat
     3301 aaagccaacg taaagaggaa gcttgcagat accagtccct ctgtgaatcg gctaataaag
     3361 agtgacagcc agataaccac aatcataaac caaaacccaa tagtgtatcc tttgagaaga
     3421 atctgttttt ggcgttcatc atatttgggc aacttctgat cgtcccttac cttagaatct
     3481 agtacttcct tgctgtctag tctcgtttgg agctttttcg ctgtctttag cccccatacc
     3541 aacaatatga gaaagctagt caatacaaaa gctacgcctc caactaagta acgttgaaaa
     3601 ctggtcttag ccagtaatgt tgctactatc acaactagat ccatcactgc tgttatgaca
     3661 atgaaacccc attgtgactt agcgagattt tgtttgctat caaacatgat tcgtttctcc
     3721 tttaaatctt tatatgacat aatataaatt attttggata ttttgtcaaa tatattttac
     3781 ataattgatt gacttaagca gcaaaaaagg attgaggatt tctcttcaat ccttataagg
     3841 gattaacggg aattctgccc aatcacaaaa actgccaggg tgttaggacc aacgtgagct
     3901 gagataactg gtcctagtgg catcataaga acatcgctaa tattctcatg cgccagcagc
     3961 tcttctcgta acttttcggc actaccctga tcgcttgtat aagagacaat aaccgtcgaa
     4021 tccgcaatat ctttttccac ttgagccacc atttctttga tggctttttg gcgtccccga
     4081 atttttgcaa tgggcaccaa tttcccttcc tcatcaatcc acaaaagagg tttgatactg
     4141 gctaaactgc ctagaaaagc tgaacctttt gacagacgcc caccacgcat caaatgaaaa
     4201 agatcatcta ctaagaaata ggtgcgcagt cgagggatca ctgcttcaac aatatcttta
     4261 gtctccagta aatttttgcc actatctctg gcttctgctg ctaaaatggt tagatagcct
     4321 tctcctcctg cagcagccaa ggtatcaaca atttcaatca ccgcatcggg ataatcttct
     4381 cgaacaagat cgcgtgccat tagagcgctt tgataggtac ccgataaaac cgaagaaaaa
     4441 gcaagataaa gcagtgcttt gttgttccta gcatgttcac gaaaaacctt ctcaaactct
     4501 cccacattaa tctgactggt ttgaggatga ctgccagctt tcatcttttt caaaaggtaa
     4561 tcactactaa tacggttagg accgaccgtt tcataaacct ctccatcgca caaaatcgtt
     4621 aatcctataa ggacaatatc atgatcttct gcccaggttt gattcaaatc agcggttgaa
     4681 tctgtcatta ttgtaaaggt cattcttact tctcctcatc cagtttttta gtttgataat
     4741 caaataatta ttgttttatt ttatcaaaaa aacccgcact tggcgagtcg taattctcat
     4801 tatcgatcta actgagacat aaaatccttc aattgaataa gctgtccttg gtcaaaaggc
     4861 cgcagtttag gcgttttgag atcaatatct ctagacattt tatctaaatc agctttgaca
     4921 gtatgaactc tttgctctaa ttctcttgca gtgacacgaa gagctccttg agaaaaaatc
     4981 gtccattgtt catttaaatc actcgtagct acttctacca tatgtatagc cgtatttaac
     5041 tctgctgcca tgcgctcaat gtagctatct gctgtttcgt cttcctctgt aaataccact
     5101 gagatataat actggtcata tcgttgcctt agccccggta catattgggc atcaaaaaca
     5161 caaataatat taatattctc aaaatgggca taatgattaa gttttgttaa aagtgtgtta
     5221 cgtgcctgat caagctgatt tgtcttaaac aactgacggg ttgattgcca aaaggcaatc
     5281 atattatacc catctactaa taatatccgt tttttcataa tttagcgcct atttcggaaa
     5341 acttcataca tgagaatagc tgccgctacg ctagcattta agctctgtac gtgaccattc
     5401 ataggaatcg taatcatttc atcaacttgc tttttgatat tggtagagat gcctttgcct
     5461 tcgttaccaa tgactaaagc aagtttacca ttagtgttcc agcaatcaga cggtgttcca
     5521 ttcatgtctg tgccaaaaat ccagaatcct cttgatttca atttatctag agtttgacta
     5581 aggttagtta ctctcgctat cggaatatgt tcaacagcac ctgtagacgt ctttgaaacc
     5641 actggagtca caccaacaga acgatgttta ggaataatca ctccacatac atttgtagca
     5701 tcagctgtcc gtaaaatcga tcctaaatta tgaggatctg tcagaccatc taaaataaga
     5761 atcaaaggat ttgcttcttg ctctgctatt tcgaggatct catcaacatc agtataggca
     5821 aaagctgata ctctcagaac aaaaccttga tgcacagctc catcagtcat ttgtgacaag
     5881 gtttttttag gcgtccatga aatagcgacc tttttttgtg tcgctaagct tttgatgtta
     5941 tccactttct ttcctcttaa atcctcttgg atataaagct tatttcctgt atttgcttga
     6001 aggctttctg taacggcatg aacaccatag acgatatcgt ttgtttcaat agtatcttta
     6061 tcttccatga gtttattata ccatgaatgc ttcaaaataa atgcatagac tcttttattt
     6121 tcgtctagat actttttgaa tagctataaa taaacctgca gctaaactga ctactagacc
     6181 taataagacc caagggaatc ctctttttga agtaggtttt acttcctctt tgtccactag
     6241 catttttgtc ttgttgctac cgtttttttg gctattacta gcagttggtg taagtttctc
     6301 atttttagta gatcgcttta aggaagttga tcgatgatta gtagctgatc tgaaaggatt
     6361 tgaagtagaa tctatttctt ggttagctga ttgatttgtt tgagcttttg tgtctacatc
     6421 gaccgaattc gtcgttgctt tagaagtatc cttttgttgc atattagttg gtgtttttct
     6481 agctttgttt tgatcaaaat gaacagttcc tgattgacca gttactgcct tttcttgagt
     6541 atgaacggtg acagttacat gatagtcacc actcttttgt ccagttaaat caaaccaagc
     6601 tgtaaaacga cgctgttgcg ctgccatagt atatacccaa ctattaatag tattatcact
     6661 agcgtcttta agtgttatcg ttgcctgagt tggttgtctg acaggttgat ctgtggtgac
     6721 tgtagcatca atataaggtt ctgttccacg catactggct tcaacactga cctgactaat
     6781 agttgattca gctataacat tatcagtgtg ctgatcttgt gcatacacag atatggcttt
     6841 gccagaagtc cccagcaaac caataagtaa agctagtgat aataagctaa ctgttttttg
     6901 catagttgtc ataataaata tctcctcttt aaattagtct taccctctca ttataaacta
     6961 ttcgaaaatg atttcattat ttttatgtct tttttgttac aaatgtatct tttttatatc
     7021 attatgtctt tttgatgtca taaaaagact taatggctct ctacctttac ttcagaccta
     7081 tgaaaataag agtctcctag gggactctta tgaggatatc aattgttgct tttctacata
     7141 ctcaatacac catctgatca attcttctaa ccgctctttt tggcccatca tatcaagata
     7201 acccataatg gcttcaaagc ctgtcgacat acgataggta ataatatcgg cattcttagc
     7261 tttagtatgg ctattggtat tgcgaccacg tttatagatg tcttcttctt tttcggtcaa
     7321 taggtgcgct tctaacatag cctgaatcaa gttggcttgt gccttagcag aaacatatct
     7381 cgttgctaaa cggtgtagct ggctaggttt cgttttacct tgaaaaatga gatgacgacg
     7441 aacatagtag gagtaaaccg catccccttc aaaggctagg gcaataccat taatcaaatt
     7501 cacatcaact ggattagtca cgcagccacc tcacaccatc tttggtatct agcagcttga
     7561 tgccttgagc tgctaactga tctcgaatgg catcagctgt ggcaaaatca cgattcgcgc
     7621 gtgcttcttg tcgcttagca atcaaggctt caatgtcaac ctcaagcacc tcttcttcaa
     7681 agataatgcc aaaaacagct aacattttct caaaagcgct tttaacaggt tctgtataag
     7741 agccagaatt aatccactta gccatgtcaa atacgacggt aatcccattt gctgtattga
     7801 aatcatcgtc catagcgtct tggaaagcta ttacaaactg ttttagctct tgctcatctg
     7861 cagtttctgt cagcggttgt tgcaaggtgt ttttaagata cttcagattg atttccgcat
     7921 catgaatagc cttttcagtg aagttaattg gttttcggta ctgttgtgtc gcaagaaaga
     7981 atcgcaaaac ctgaccatca acggtttgta acatgtcatg aacggtcaca aaattaccta
     8041 gtgacttgga cattttttcg ctatcaacag tgacaaaccc attgtgcatc caatagttag
     8101 caaaagtctt gcctgttttg gcctcagatt gggcaatttc attggtatga tgaggaaatt
     8161 ccaaatcagc tccgccacca tgaatatcaa tggtatcacc aagaatttca gtagccatca
     8221 cagaacattc aatgtgccag cctggacgac caaaccccca ggggctatcc caagaaacct
     8281 cacctgcctt agctgatttc caaagagcaa agtccagtgg attttctttt aaagctgtct
     8341 cagcatctgt tcgaccactg gctccaactt caagttctga cagggtttta ttagctagct
     8401 tagcataatg ctctgacttt tccacgcgga aatacacatc tccatctgct tcgtaagcaa
     8461 aatctttttc aatgagactt tcaacaaatg aaatgatttc tgctatataa tccataacac
     8521 gaggattttg tgtggctggc ttaacaccga gtgccttggt atcttctata aaagctgcaa
     8581 taaagcgatc tgataattct ttaggagaaa cacctgcttg agtagcagct ttgataattt
     8641 tatcatcgac atcggtaaaa ttggaaatgt aattgacctg gtagccagta tactcaaaat
     8701 aacgtcgaat ggtgtcaaaa gcgaccgctg atctagcatt tccaatatga atataattat
     8761 agaccgtcgg tccacaaacg tacatattga ctgtattttc agtcaaaggt acaaacttgc
     8821 ggagcgaacg ggtcatggta tcataaattt taatcatgta aactccttat tcaatagcct
     8881 ggcattagtt gtcatcttga tatcctaagt ctttttctga aatgctgttc caccttttca
     8941 acaatttgat attaataacc aaacctatta accaaaatag ggtcaaaatg aagtatataa
     9001 tcgctttaac catgtcttgt ctcagtaaaa aaattagact agccaccagt gtaatactaa
     9061 aaacaaggat agacaaactc ataatgacat acagcccaac tcttatttta ttcatgaggc
     9121 tcctttatta acgaaaaacc ttcacaacct gtgaaggctt cagaaagtag acaaacccac
     9181 ttttatgctc aaaaagcatg aatagatcta ctgatttgac atcaggtttc ctatttctgc
     9241 atgtggggtg agccaaaaca gctgcttttt gttgtggtaa aagtggcatc aatattgaca
     9301 gtgataccca tacggatttt tgagatctta ggctcaaaac taaggcatgg aattccaaag
     9361 gaagtcgctg ccgtaagcac cacctgagaa aaagtatcac aatagactgt ttcttcagtc
     9421 taagacttgc ttagttgatg ctcttttacg cgttttttgt attttgtaac attcccatat
     9481 cactacactc agcaaccaaa ctgatagggc tagctaagtt caacattact ctactagaaa
     9541 gtcccaaaat attttattgg aagtcatcag cgcttctttt tgttattttc cgatcatttt
     9601 gacaactgat aagagacctc tcgttgtttt tgtaaacttt gaatttgacg attatctttt
     9661 tgcccatgca ctcgtactat cttagctggc acacctacaa ctgtcacgtc ttcaggaaca
     9721 tctgataaca caacagctgc tgcccctact ttagcatttg ctccgatgtc aataggtcca
     9781 atcacttggg catgtgccga aattaaggca ccttgtcgaa tcgttggatg acgcttgcca
     9841 caatcttttc cggttccacc tagggtcacc ccatgataaa gcatcacacc tttttcaaca
     9901 attgctgtct ctccaataac aagaccagca ccatggtcaa tgaagactcc aggagcgatt
     9961 tgtgctccag gatggatttc aatttgtgtc caaaatcgcc aaaattggct atgcattctg
    10021 gctagtaatt taaaatgatg tcgccaaaga aaatgagaca aacgatgagc agctagggct
    10081 ttgatgcctg gataagttag gataacctca agactattac gagcagctgg gtctagagct
    10141 ttaacaatag ctatactttc tttccaccaa cccatactaa ctcctacgac ttatctaatt
    10201 ctgaccaatc aacaagaatt tgttggtcat caaaaggatt gatggcgaca ccgaacgttt
    10261 catcaagctc gttttcacca tttcttggtt gataaatatc atcaatcgtc caagtcagaa
    10321 tcactccttc agctttcctg aaaaggcctc caaaatcgtc ttggttgtac catttcgcaa
    10381 agctttgaag atttgaaaag gcgggaacat agctttttcc ttcaggcgtt gacatggtag
    10441 gaaaaaggcg gtcgtaatgg ttattatctt taggataaac aaaggcagga acaaggtaaa
    10501 ccttttccat cgtatctgcc gccacattgt catcactcat aagcgtattt aacacagtgg
    10561 tgtaatggtt cataaattga atcatatcac tgcttttgaa aatggtagaa ttcccaaaat
    10621 ctcccttttt cttaaggtta aagacaagac ctgctgcacc agatgtaatg acttcttcta
    10681 gcactgcaag agctgaacgc tccagccaat actgactttg agcactcttt tgttcttctt
    10741 taaagcgagc catatcttcc ttatcggtaa aaacaggggt aaccttgaca ccttcgactt
    10801 ctatcacgta gggttcttta gcagcccaga caggaaaatt atggaaagcg ttgacaagcg
    10861 caaggctatc caaaaagtta tcaggcgcat tgataaaagc tcttaagcga atatctaatt
    10921 catttgattt agtcatgttt ttcttctttt ttctctggtt ttggtgggcg tggaatcaaa
    10981 gctttcattg aggcatccac acgacccttc tcatcaatct taatgacctt aacatcaacg
    11041 tcttcaccca cttcaaggac atctgacacg ttggtcgttc tagtccaagc aatttcagaa
    11101 atgtgaacaa gtgcatctgt cttgtcaaag aggttgacaa aggcaccaaa tttctcaata
    11161 cggacaacct tagcatggta aacttcgcct accttagctt cacgtactag gccagcgatg
    11221 atttctttag tgcggttaat ggcagcttgg tcacttgagt agatagagac atttccttcg
    11281 tcatcaatat caattttgac acccgtttcg gcaataatct tgtcaatagt ttcgccacct
    11341 ttaccgataa ccactttgat tttgtctacg tcaatcttga tggtgtcaat tttcggtgca
    11401 gttggggcta actcaggacg tggttcagcg atagttgctt caatcacatc aaggatttca
    11461 aagcgagctt ttttagcttg tgcaagagct tcttctagga tttgaggcgt aatccctgca
    11521 atcttaatat ccatttgaag ggctgtaatc ccttcacgag ttcctgccac tttaaagtcc
    11581 atatctccaa agtggtcttc aagaccttgg atatcggtca agacagtata atttgtccca
    11641 tcggaaatga gacccatggc aatccctgcc acaggagcct tgattggtac accaccagcc
    11701 atcagagcaa gggtaccagc acagatagaa gcctgagaag acgaaccgtt agattctaga
    11761 acttcggcaa ctaaacgaat ggcataagga aactcttcta agcttggcaa gacttgttca
    11821 agagcgcgtt cacctaaggc accgtgtcca atttcacgac gaccagcagc tccgtaacga
    11881 cccgtttcac caactgaata ttgcgggaaa ttgtagtgat gcaagaagcg ttttttgtac
    11941 tctggggcca gaccatcaat gatttgagtt tctcccattg gtgccaaggt caaaattgat
    12001 agcgcctgag tttgaccgcg cgtaaaaaga cctgatccat ggacctttgg caagaagtca
    12061 accacagcat caagcggacg aatttcgtca atcttacgac cgtcaggacg aatcttgtcc
    12121 tctgtaatga ggcggcgcac ttcagcatgc tccatttgtt caaggatttc tgccacgtca
    12181 cgcataatgg ttgctaggtt ttcgtcttct gcgtaacgtt cttcgtattc agccttaacc
    12241 atttctttaa cggcttctgt ggctgcttca cgcgcttttt tctcttcaac ttgaacagct
    12301 ttttgcagtt gagcattgta cttagccaca atatcagctt gtaagtctgc atcaacctgt
    12361 aacagttcaa cctcagcttt ctctttacca acaactgcta cgatttgttc ttggaaggca
    12421 attaattctt gaatggcttg gtgacctttt aaaagagcct caagcatgat gtcttctgat
    12481 aattctttgg caccagattc aaccatgttg atggcttctt tgctaccagc aacggttaat
    12541 tctaagagcg acgcttccat ttgttcttta tctgggttga tgatgaactc gccatcaata
    12601 tagccaactt gaacacctgc aatcggtcca ttaaatggaa tatctgagat ggacaaggcc
    12661 agagatgagc caaacatcgc tgccattgga gcgctagcat tttcatcata agaaaggaca
    12721 gtgttaatca cttgtacttc gttacggaaa ccttcggcaa acatgggacg aatcgggcgg
    12781 tcaatcaagc gagctgtcaa ggtcgcatct gttgaagggc gtccctcacg tttcatgaaa
    12841 cctcctggga atttaccagc ggcatacatt ttttcttcgt aattaacttg aagagggaag
    12901 aaatcacccg ttgccatttt cttagacatg acagctgcgg tcaggacagt tgagtcgcca
    12961 taacgaacaa cggttgcccc attggcttgc ttagcgactt gaccaacttc aacaacaagg
    13021 ggtttccctg caaatgttgt tgtaaaggtt tgttttgaca tatattctcc ttttaatttt
    13081 cagaggggta aagaggtacc tgttctctgc ttagattcaa ttgttatacg ttgttttgct
    13141 acaaaaagca acctgccacg cttgtcacac gctcctcttt gtatcaaacg acgtatctgc
    13201 tacattttac cataaaatag cagaaatttc actgtcagat cgcaatctct tgaaatgtcc
    13261 ttgaaaaata ttaaacgaaa cttatactct tttaacaaag caaaaaaagc aggttggtat
    13321 gcctgctttc gtctcctaat attaatgatt gtcatcctct aaaattttaa agccgaggct
    13381 ggtactgtct ttcagaaaca tttgcacggt ttcaagggaa tagtcttcta aatcttttcc
    13441 ttgaagaggc aacttggcaa tcaaatcagt ggtacaagtg ataatgtcca cgcctagctg
    13501 atcagcttga tagatattat aagtttcacg aggactagcc cacaacaatt cgaccccttc
    13561 tttttgaaga caaatgcgta gagcttcttc catgattggc attgggtcaa cgccggtatc
    13621 tgcaatacga ccagcaaaaa cagacacaat agctggcact ccagcggtta aatgatcaac
    13681 cacagcttga gtctgctcaa ttgtaaaaat ggctgtgacg tttaacttaa tgcctttggc
    13741 tgacaacttt tgaataacgg aacaagttga ttcgcctgtt gaagtagtga ctggaatttt
    13801 cacatagaca ttgtcaccta gactggcaat tttctcagct tccttttcca tgcttgcgag
    13861 atcatccgca aagacttcaa aggacactgg gtagtctgta attttggcaa gcacttcttt
    13921 ggcaaagcca atataagaac tgatgcctgc ttttttcatc aaacttggat tggttgtaaa
    13981 gcctgtgacc aaacctgttt gtaaatcctt taacatggtc tctaagaccg caccgtctga
    14041 aaagacttta acgtttagtt tttttgtcat catgtcctcc ttttagattt cttctgaaac
    14101 attattgatg gcctcttgag attttgacag aaagtttgga agcaccaata caatagcaag
    14161 cacagcaacc accaagtaaa taccgatttg accaatcgac gttcctaagc ggcccagtaa
    14221 aatccctaaa acaccgaaat ccacatctcc aaaggtcgtg tttgaaaaac caagtttacc
    14281 aagtacaggt agaagcatgg ctggcaagaa ggcgagtaat aagccattag cgaaagcacc
    14341 aatcatcgca ccgcgacgac cacctgttga atttccaaaa atccctgctg ttgcaccaca
    14401 gaagaaatga ggcaccatac ctgggataat caggacacca cctgcaactc ctaaaatcaa
    14461 catcccaagt aagccaccaa cgaaacttga cgcaaaaccg atgataacag cggttggcgc
    14521 ataagggaag aataccgcac agtctacagc tggaatagca tttggaatta atttgttagc
    14581 aataccttgg aaggctggaa tcaagtcagc caaaatcatg cgaacaccgg cgtagacaat
    14641 tgccacacca accgcaaagg taagaccact cttaatagca aagataaatg ggttttgacc
    14701 tgctgctaat tcttctgtta ctgaagcatt cctcaaaaca gaagccactg ttgccactaa
    14761 gtagaaaatc accatgataa gtcctgttga aatggtggtg ttgcgtaaga aactccattt
    14821 ttcagaaatc tgaaggtcct cggtatcttt actgtctttg ccaaccttgc taccaaccca
    14881 ggcagaaagg taatagccca aactaccaaa gtgtcccatg gcaatttcat ctccgtcagt
    14941 cactttcaac gtatactgtt gaccaatggc tggcgaaata gctgaccaag ctcccaagag
    15001 gaacccacct aagataatca aaagacttcc tttgaaacca acagctccaa gcacggcgga
    15061 caagagacaa gccataaaga agctgtgatg tcctgttaag aaaatgtatt tgaagcgggt
    15121 aaaacgagca aaagtcaaat tgagcaataa cccaaccacc aagatggaca tggtttccac
    15181 acccagaatc ttttgagcga cagaagtgac ggcttcatta tttggcacta cgccagtgat
    15241 gctaaaaccg tgctcaatga gtttggcaag agggtctaga ttggtcacaa taacacccgc
    15301 ccctgcagca agcataaggt atcccaaaat tggccccaag gttcctgtca acaccttatg
    15361 agcaggtgtt ttcaaagcca ccaaccctgc aaaggcaatc aagcccatta aaaatgcagg
    15421 ttcttttaaa atatctcgaa taaatgataa taatgcttcc atagtatctt cctatctcct
    15481 ttaaaggctt attccttgcc actcatttcc ttgagaaccg gaaccaaatc ttcttcaatt
    15541 tttttacgat tggtatagct tttaacgata caaaccttgg catcctctgg aaaaatatct
    15601 ttaaattctt taacggtaac aaacaaatct gcaccctttc caacagccgc attggaatca
    15661 caggattctg catccacatc aatacccaaa ctagatgcga tagcttctac tttcatgcga
    15721 agtaacaagc tactaccaat accgtttcca caaaccgtta caattttaat catgtgattc
    15781 atctcctttt tcaatcatat ggctaatcag actcattatt tcctcagtat tcttagcctc
    15841 aattaattgc tcaatatgtt catcgtcatc taaaatcaac gacagttcct gcaaagcctt
    15901 caaatgtgaa ctagaatcca cagcagacaa aacaaaaatc aattgcactt gcttgtctgg
    15961 atcaccttct tgcttcaaat caaagctgac aggttctttt agttgtaaga gagacatgcc
    16021 taactgccgc gttccttttt caggcgccgc atgaggaaca gccaccttag gggctaaaac
    16081 aatataggcc ccaagctcat tgaccgaatc aatcatgcca tcgatataag atgtttcaat
    16141 catctggtgt tctagtaaag gctgagcagc caaacgaatc gcttcctgcc aatccgaaac
    16201 agcatcagcc tgcctaataa aatctgctgt taacaagtcc aatagtccta gacctccttt
    16261 ttcaatcgtt aaatggttac caatcaggta acgggataaa tcactaacaa gtccttcttt
    16321 attagtaatc gtggtgtgtt tatgaataat actcaacagg tcatccaaag cgaattgctc
    16381 tgacaaggga agatgaaagt catcacagac catctttttc aacatcattt tctcaacagg
    16441 ccccatcaaa gcttgcgtca cataaacagg tttagcacag tcaaaagcta ctgttgaaaa
    16501 aatcaaatca aaggaagctg gatccaacaa ctttattttg tccagctggt gaatcctaat
    16561 aaactgtagc tgtggaaaaa gttccttcaa ggttgcctcc aacatcagag acgaactaat
    16621 gccattaggg caaacagata aggctaccag ctgattgctc ggcctcttct taggggcctg
    16681 caaccatctc ccaaaatgaa tggtaaaata agcaacctca tcttcattaa ctgatttccc
    16741 caattgtttt tctagaggag aaaggctgcg cttgaccaag taaaagagag attcataatc
    16801 ttgtttgatt tgttccttca aaggattggt caagttaatg tcgtagtaaa gtcggtaata
    16861 agctggtaaa aggtgactgt ataggttctg acgaagttct ggggtatcgg tgatagacaa
    16921 acccgtatta accgccaccg aattgatgat ttcctccata atatcataga taggctgctg
    16981 aaagactaag tctaagtcac cttgaataca accaagtaac ctagattgaa ccaaatattt
    17041 ttcttcggtt agactgggga agttgaccaa taactggtcc accatcgttt caacagctgg
    17101 cgaagctaaa ggaaaagtgt ccactgcttc accccttaca tcccgtgaaa atggtcgatg
    17161 agcgagaagg cagaaaaaat aaatcaaatc cctcaatttc tccgaaataa aagttaagtg
    17221 gttctccctg ctcagttcct ccaacgttgc tgccatcacc tttttctggt cgggcaaacc
    17281 aagttcatgc aataagtaac tgaacaccca aggtcctgat gtgacttgca aaagagaact
    17341 gaccgctgat tctaagagac gccgcaaggc taagggttct ccttcaaaaa aataaccatc
    17401 ctggcgattg taagctaaag ttacctgaaa agcttgtacc cgatcacgca ccaagtttaa
    17461 atcagcaata acggaatttc tgctgagccg taacaaactt tccaagtggt taatggagat
    17521 aaattcttta gccatcatga tgtaaatgat aatcatgtcc agacgctcat cttgaaagag
    17581 atagtcgtct aaggtaggta gcatgcgatt caacaagtcc ttttcagtaa ggtctggcgg
    17641 ataaaccagc tgcctgtcca gagcaataag gggtaactga tagagtgaca acgtgtcatt
    17701 gattttagca atatctgcca gtatatgacg tttgcttgtc tttaatatgg attcaaatgc
    17761 ctccagtgaa tgtcctttat atttagaaaa gagttgatag tttctgatga gctcatgaga
    17821 tagcatgtca tctcctccta taatgtcagt ataaaatgaa gcgttttcat tttcaagaca
    17881 aattgagtcc aattgtctct cgtcattttt gactgcaatt ggactcaaca ggtgatatca
    17941 ttgtaacaag ttcctcaaaa ggtggcaaaa aataggcaaa tctgggaatt gattcccttt
    18001 ttcttcatga tacaacaaaa gatacctcat cactgagata ccttttgttg ctaaataaaa
    18061 tcatcaactg ctaaaaaaac agtcttagac cattctagcc aaatgtgaaa actaacaaac
    18121 aacatgtcaa aaatgaaaaa gcccactgca taagcagagg actttttaaa cttgaaatta
    18181 acgacgaagt ccaagagatt ggatcaactc acggtaacgg ttaacatctg tacgacgtag
    18241 gtatgccaac aagttacgac ggtgaccaat ttttttcatc aatccacggt aagtagcgtg
    18301 gtcttttttg tgttctttga tgtggctgtt caagtggttg atttcccatg taagaactgc
    18361 aacttgaact tctactgaac cagtatcgcc ttcgtgacgt gcatattgag caatgatttc
    18421 attttttttc tcttttgaga ttgccataat atattctcct ttttttgctt aatccgagtc
    18481 ctaggattgg cactcctaaa accaagaaaa agttggtttg tctttcgacc actctattct
    18541 agcagattca taatattagg tcaaccgtta tcctattatt ctgagctctc acatccaaac
    18601 cacctcacta ggcttaaaaa ggttatctgg tcgttgaatg tgctcttttt gctttaacca
    18661 aaccaccata agcaacagaa ctagcaaggc caaatagcca agaggctgtt tttaaatcag
    18721 aataaacctg tgcgattgct tgcctaataa gataaccagc taaaagaggt aagatggctg
    18781 atcccagcac ttcagctgat aagcggtagc ctagcatagt cattcatttc tttccaacaa
    18841 agtgttcact gataatatga attgctctag tattaacaaa cccaatccct aatccatgaa
    18901 cacagcttat taaccgatgc gacctgcctt tatgacatag gtcaagagtt gacacaaaat
    18961 gtttgccaac aaaacattac ctataccgtc aaagagcgct ttgcttccta tacccttgaa
    19021 gcgcaagcca accaagaggt tcatctaaac cttacattat tagcaaaccg ttttggaaca
    19081 agcgatcgcc atcttaagca cgtacttaag cagcccattt ttcagaggat tatcgaacgc
    19141 atctgcttca aaacctgtcg tattgccgat ttagacgctc taactcaatt aagtgttaat
    19201 aactagacct ctgaggtatg cagcccatta acttataata gcaatcactc taaccactac
    19261 ctgacttttc tttaaaaaag aaatgatttg tgatatagta aatccagtta aaaaaaaagt
    19321 aaaggagtag acatgtctgc acaagataaa ttaattaaac ctagtcatct gattaccatg
    19381 gatgacatta ttcgtgaagg gaatccaact ttaagagctg tcgccaaaga agtgagctta
    19441 cccttgtgtg atgaggatat cctattagga gaaaagatga tgcaattttt gaagcattct
    19501 caagatcctg taatggcgga aaaacttggt cttagggccg gtgttggtct agcagctcct
    19561 cagattgatg tgtcaaaacg tatcatcgct gtgctcgttc ctaatcttcc tgataaagaa
    19621 ggaaatcctc ctaaagaagc ctatagctgg caagaagtcc tgtataatcc taaaatcgtt
    19681 tctcattctg ttcaagatgc agccctttct gatggcgaag gttgtttatc tgtcgatcgt
    19741 gttgttgaag ggtatgtggt tcgccatgct cgtgttacag tagactacta tgataaagaa
    19801 ggacaacagc atcgcatcaa attaaaggga tacaacgcta ttgtggttca acatgaaatt
    19861 gaccatatca atggtgtttt gttctacgat cggatcaatg ctaaaaaccc ctttgaaacc
    19921 aaagaagagc tcttgatttt agactaacaa aaaaaggcta aagggccttt tttgttagtc
    19981 taaatagccg ctaaaagagc ctctgcctga gatagcaagt ttgcttttat ctcttttgaa
    20041 atctcaagcc ttcctgttcc ccaggcatca ggattcacag ttgcttttgt aaactctcct
    20101 gctactgaag ttcggataaa cggcaatagt gctttaaact gatcaaatac ttgatcatgc
    20161 ccgccatttg caactgaaga gaccgtaacc accttaccgc caatagcaga tgggcccgtc
    20221 ggatcagaca aatcaagagc acgagacaac cagtctagca ggtttttaac agaacctgga
    20281 atagagaagt tgtaaactgg tgtaaaaatc cagatagcat ccgctgactg aacagcttga
    20341 cgagcgtcaa caactggtaa aggtgcatta gcttcgatat cttgattcaa aacaggaacg
    20401 tctttccaat ttaagtaaga tacaactgct tgatgttcca gagctttttg tgcttgagcc
    20461 gctaattgat ggttaaaaga cccttcacga agcgagccaa caataaataa aatatgtttc
    20521 atctctttgt cctctttttt ttaaagatcg ttttaaagct ttctaaaagt ggtatttcat
    20581 tgatacttct ttttgaaatt tatcactatg tagtaattat atcttctcta agaagagctg
    20641 taaagagatt tgtttataat tgtttgaact ttttgagaag ttcaatcaga caaagttgtt
    20701 ctttttcagt caaaacagca aaggcttctt ccacccgtgc gacgtgttta ggcaaaacag
    20761 cttcaatcaa gcgattaccc ttatctgtta aggtaaccac ataggctctt ttatcagttt
    20821 tgtctttgca cttgctaatc cagccattgc gttccatatt atttagtaca acagtcatat
    20881 tccccgaagt cgctaacaaa gaatcaatca agtgattgat tcgcatacag cctttagtat
    20941 atagtacttc aagcacactg aactgcgtgg ctgttaaatc tgccttttta aaaatatcag
    21001 ccccaaaagc atctagcgtt cgctgagctt tacgaaacac caccatggct ttcaaagcgg
    21061 tgttcttatc taaatgactc atttgatttc ctcgctttat acggtatgtc acagtttagt
    21121 atcagagaga ttagaaagtg atcttgtccc tcttctttta tgactttgca tattactacc
    21181 atcatgataa tctagaaaaa gtcatcaaaa agacttaatt gattatcttc tggcatattt
    21241 cctaaaatac tcatctcatc cattttctca acgagcgttg acgatgcccc gcctcgttta
    21301 cgcaattcca ttttagagag gaattcgcct tcttgacgag ctttaacgat ttgcttggcc
    21361 acgttttcac ccagaccttc taacgctata aatggaggga taagggtatc tcctttgatt
    21421 tggaattcta tagcatcact tttgtaaagg tctaatttgc caaacttaaa gccgcgttct
    21481 aacatttcgt tgacaatctc caaggttgta aagaggtcat tttccacatt ggtagcttca
    21541 ttatttttac gttttatagt aatatcttcc attcttgctt taacagcatc taaaccacca
    21601 ctcatggttt ttaattcaaa agccttcgca cgaatagaga aataagcaca ataatacata
    21661 atggggtggt gcaccttgaa ataagccacc cgaagggcca tcaaaacata agctgccgca
    21721 tgggctttag ggaacatgta cttgattttt ccacacgatt caatgtacca gtcgggcaca
    21781 ttgttttctc gcatggcatc aatatagcca ttacgttctt cctcagaaat tttcagccat
    21841 aatcccttac gcacacgctc cataatggta aaggccattt tcggttctaa gcctgcgtgc
    21901 atgaggtaaa ccatgatgtc gtcacgacaa ccgataacgg tttttagggt cgcaatgcct
    21961 tctttaatca aatcttgcgc attaccaagc caaacatcgg ttccatgaga tagtccagac
    22021 aactgcaaaa gctccgcaaa agtggtcgga tgcgtttcgt taaccatgcc gcgaacaaag
    22081 ttggtcccaa attctggaat gcctaacata ccagtcggtg tcccaatttg ttccggggta
    22141 acgcccaaaa tctctgtccc agaaaagaga gccataacac ctggatcatc agcagggata
    22201 gtaataggat caatgcccga taaatcctga agtttacgaa tcatggtcgg atcatcatgc
    22261 cctaggatat caagtttcaa gacgttttca tcaatatcat ggaagttaaa gtgagttgtc
    22321 tgccaagaag ccgttacatc atcggctgga tattgcacgg gggtaaaatc ataaacatcc
    22381 atgtaattag gaataacaac aatcccccca gggtgctgcc cagtcgttcg tttcacacca
    22441 gcagcacctg ctgctagacg atccacctca gcatcccgat agaacttgcc atagtcgcgt
    22501 tcatagcctt tgacaaatcc ataagctgtt ttttctgcta cggtaccaac tgttccagca
    22561 cgaaaggcgt attcgtcacc aaaaatatct cggacatcta aatgggcact gggctgatca
    22621 tcaccagaga agttcaaatc aatatcgggc accttatccc catcaaaccc aagaaaggtc
    22681 tcaaagggaa tgtcttgccc atctttttga taaggggtgc cacattttgg acaaggctta
    22741 ttaggcaaat catagccaga tccaactgac ccatctgtga taaattcaga atgttggcag
    22801 gacgggcaaa cgtagtgagg cggcatagga ttaacctcgg taatcccaat catggttgcc
    22861 acaaaactag accctacaga tcccctagaa ccaactaggt agcctcgctc atttgaccgg
    22921 ttaacaagca tttgggaagc gagataaatc acagcaaaac cgttccccaa gatagaggtt
    22981 agctcttttt caatgcgtaa atcaataata tctgggagag gattaccata aatttcaaag
    23041 gctttttgat aggttaattc ggcaaccgtc tcttcggcct tgtcaatata cggggtgtaa
    23101 agatcgccct taaccacttc cacttcctca atacggtccg caaaatcctg agtattttgc
    23161 acaaccactt gataagcgag gtcttttcca agaaaggcaa actcatccag catttcattg
    23221 gttgttctaa agtgcgcttt aggtagagga gcaggttggg ccccttcccc acggccgatt
    23281 gttctattaa tcatggcacc ctgaccgaga ctacgcacaa taatttcacg gtaaatctct
    23341 tcttcaggct ctagataatg cacattccca gtggcaagca caggtttctt agctcgtttc
    23401 cctacttcaa tgaggtcacg aatcacctgc tcaatacctg cttgatcttt gattaattca
    23461 cggacaacca atggctggta aatggctggt ggcatgattt cgataaaatc ataatactta
    23521 gccaaatcaa ccgccgcatc aattccttta gtcagaacgg catcaaaaac ctcgccgtca
    23581 gaacaagctg ttcctagtaa caacccctct ctgtgagcat ctaagacggt tcttggaata
    23641 cgcggcaccc cttcaaaata tttgatattg gaaaggctga ccagcttaaa catattttta
    23701 agaccaacct gattttgcac atagatagtc gcatgcttaa tccgcgcttt tttgtaagaa
    23761 tcctcagcca ccaaatctgt attgagttgc aaaagatttt tgatgccatg cttttctctg
    23821 gcatctctta gaaaaataaa caaaagacgt cctgtggctt ccgcgtcgta attggccata
    23881 tgatggtggt ctagactcac ttggaaacgc ttggtgagcg gtcccaaacc gtgacgtttg
    23941 tattcaggat acaaatttct tgcgaattct aaggtatcaa tcacaggctg tgtgattttg
    24001 ggcaagtcat ggcgttcata attggcgttc ataaagccca cgtcaaaact ggcgttgtgg
    24061 gcaaccaaga tactatcttt gcaaaagtcc tgaaaagctt ttaggacggt aactaatggc
    24121 ttggcgccct gcaaatgctt gtcggtaatc cctgtcaatt cggtggtaaa ggcagaaaga
    24181 ggatgcccag gatcaatgaa ttcatcaaac tgctctacaa tatttccttt aaacattttg
    24241 gaagccgcaa tctgaatcag gtcattattc atagcagata gacctgtggt ttccacgtca
    24301 aagaccacat aggtagcttc gtgcaaatcc atatcaacag gatcataaga aataggcacc
    24361 ttgtcctcaa caatattggc ttctagacca aaaatagcct taattccagc tttgcgagcc
    24421 ctatggtagc catgaggaaa actttgcaca ttagcatggt cggtgatagc aaccgccttg
    24481 tgtccccact tggctgccgt atcaatcaag ctttctactg tcggtaaggc atccatggta
    24541 gacatattgg tgtgggcatg aagttcgacc cgcttttgcc cttctggcat caggtctttg
    24601 cggtcatgat ggacaatttc tttgacctgc tgaacattca tggtaagact cttcgtaaaa
    24661 ggattggtct caatattacc ttgtacccgt aaccaagccc ccttagcaat catgtcaaat
    24721 ttacggagct cctcatcgtc tttggcccat ttttggagag caaacgagga ggtataatct
    24781 gtcattttaa agttgatgat atggcgacct gtcctagtgg tcttgcgctc cacatcaaaa
    24841 accatcccct caaagacaat tcggttttct tcggtctcaa tctcaatcat tggtgtgatg
    24901 gtcgcttttt caaatcctgc ctgacgctta gctgctcgtt ccttgtagtc aaacttagga
    24961 gcaggtgtgg cttcctcaac tggtggcatc atggcttcaa gggatttttg ggcttccaaa
    25021 ttatcctgca cagccttttt cacaagagcc tgacgactgg aaacaaaatc cttggtcaaa
    25081 tgctcagtca tctcctgatc tgacaccata tctatggtca aggtgccaaa gccaaaggct
    25141 tctaattgct tgaccaaatt aggcagatga ttgtttctaa aatgatcgtt attcacaaaa
    25201 cctggcgctg caataatgag tttgtcatcc tcataagtca ctttgagctt tgagaaagaa
    25261 gatttaaaac tagcactatt acacggtgca tgctcaaaag cttcttggta ataagcttga
    25321 agcagatcat ctgaataatc cacctgagca gcttggatat caaaggttac cttaatgtca
    25381 gccgcctcaa aagttcttat caaacgatca tgcaattcac gataagttgc aatcggtaaa
    25441 accgccgcaa aggcaaaatg aaattcccac aagcgtgaca ccgaatgcac ctttacctcg
    25501 ataatatcag cagatgaaaa ggcacttgaa cgtctcatgt caagtggcat ttctatctgg
    25561 tccatcaatt tagcgaataa atctgacata attttctcct gattttacta ggctttattt
    25621 tatcacaatt caagatgatt ttcgagtttt agatggaaag aaaagcaggg ctttcttctg
    25681 acgttctttg gctaatttat tccaagtgat agctcagctg aggtcatttt tttgtcaatc
    25741 ttttatcgcc aagcgctttt ggtaaagcaa aagccactct gtatcagaca agttcacagc
    25801 aaaaagaggc cactctaaac aggcagcctc ttcatttctt ttttagtgtt cttttgttaa
    25861 aatttcaagg gtttcgatga ggttttctgc attaacttca atgctggcac ctgttgcctt
    25921 gattttaatt tccacgatac cttcagcggc ttttttacca acagtgacac gaattggcaa
    25981 accaatcaaa tcgctatcag agaatttcga accgacgcgt tcattacggt catctgtcaa
    26041 gacatcatac cctttagccg tcaagtcagc ttctaacttc gccgtcaagt cttgggccac
    26101 ttgatctttg acattaactg taatcaaatg cacgtcaaat ggggctaatt ctttagggaa
    26161 gttaacaccc caagcataac ggtaatcgcc ttttggtgtc ttgttcacaa agagacgggc
    26221 atgctgttca ataacagcag acaaaatgcg gctaaccccg ataccataac aacccatcac
    26281 aattggaacg gctctgccat tttcatcaag aatcgttgct cccatgctgt ctgaataacg
    26341 agtgcccaac ttgaagatat gaccgacttc gataccacgc gcaaactgga gaacaccatg
    26401 accatccgga gacatttccc cttctttgac ttcacgaata tctacatatt cagcctggaa
    26461 gtcacgtcct ggattgactc ctgtcacatg gaaaccatcc ttgttagctc ctgcaacggc
    26521 attggtaagg ttttgcactt tgcggtcagc cacaatgcga ctaccttgtg ccaagttgac
    26581 aggcccaagt gagccaaaac ctgcaccaaa gaaagcacgg gcttcttctt cactagctgg
    26641 ctctaaaaaa tcagcagcta gatagttttt taatttaacg gtattgatat ggtcatttcc
    26701 aactagcaaa gcaacaacag gttcattatc tgccacaaaa agcaaggttt tgatggtttg
    26761 tgtttcatct actgaaagaa aagcagccac ttcatcaatc gttttgcaat gtggtgtctc
    26821 aacttctgcc aaggcatctt cagcagctac ttttgaggac ggtttgtatt cgttagttgc
    26881 catctcaagg ttggcagcgt agcttgattc tgttgaataa gcaatagtat cttcacctga
    26941 aatcatccaa gcagccaatt ctgccttaat ctcttctaag acctcttttg gaatatcatc
    27001 cattgacgca atagacttgt caagaaccac ccagcgatca aggtccgtac gagctggtgt
    27061 aatggccata aattcttggg aatctttccc ccccatggca ccgccatctc caataatccc
    27121 tttgaaatct agaccagctc tggtaaaaat agcttcgtaa gcttgacgat aatcttcgta
    27181 ggtcacatct aaatcttcat agttgtgatg gaaactatag ccgtctttca tgataaactc
    27241 acgggtacga agcaagccat tacgcggacg tttttcatca cgatacttag actgaatttg
    27301 ataaaggttt aagggcaatt gcttgtaaga tttgaccgca tcacgcacca aagtcgtaaa
    27361 ggtttcttcg tgggtcggac ccaagataaa gtctgagtta tcacggtttt taagcttata
    27421 gaggtcctct ccataggtct cataacggcc tgattcacgc cagagatcag ctgtcaaaag
    27481 agctggtgcc aacatttcaa cagcaccgat cttttcaaac tcttcacgca tgatggtctt
    27541 gaatttctca atggtacgat ttgccagtgg taaataagca tagataccag cagaaacttg
    27601 gcgcacataa ccggcacgca ccataagcgc gtggctgata acctgggcat cacttggcat
    27661 ttcgcgcaag gttgggataa gcagtttact ttgtttcata gattattccg ttttaataca
    27721 tccccttatc ctcaaaccct tctggaaaca tcaagtaaga gtgaagcggg attattcaat
    27781 aatttgaatg tgtattcctt tcattttgag taagacctca acccttgcaa atcttagggt
    27841 tgaggtcttt ttcgatcttt aacatgataa agtcagctta agatgaccta tcttgattag
    27901 aaaaagacgc gcatgatatc attccatgtc acagcaatca tcaatacgac catgatagca
    27961 accccagcta gggtgatata ggcctctgtt tcttgcttga taggcttgcg acgaatggct
    28021 tcaatgatat tcatcaagat ttttccccca tcaagtgcag gaatcggaat caggttaaag
    28081 atccctaaat tgatcgaaag cattgccata agagataaga ctgactctaa gccgttttga
    28141 gcagcctgat tggacatgtc atacatggca acaggtccac ctaatttatt gagactaaag
    28201 ccaatgatca atcctttcaa agcattcaaa atagtaaacg ccccactcca agctaattct
    28261 agaccgccta agagcttatc cttaaatcct gttttcagac tcgccttaac tcctatcgtg
    28321 tatgtctttg catgcttttg tggtttcaca gccaccgttt ttaagcgctg atgtgacttg
    28381 taggtgacct taatggtctg tgacgggcct agatcccgtg ttgcaaggtc tacagcctca
    28441 gtaagatcat tccagctagt caccttgtaa ccattaattg cgacaatttg gtcattatct
    28501 cgaagaccag ccttagctgc tgctccattt tcttgaacac ggacatgatt gctactaaaa
    28561 tctggcatcc caccttgtaa aaagaccaag aggataaaaa ccacaatccc taaaataaaa
    28621 ttattcatgg gacctgcaaa attggtgatt aaacgtccgc caatactagc attttgatat
    28681 tgaacatcaa gcggagcaat tcttatctca gtaccgtctt cttcaacaat agtagcatcg
    28741 tgagcgacct tatatgtctt ggtttcttct aaaaccaagc cagtaatact cagctgatct
    28801 tctaagtcat agcctgtaac atgcattggg agactcgttg ggtctaactt actttgagac
    28861 aagttgatgc gcttaacaaa accttgctca ttaagggtta aacttgctgg agtacctgtt
    28921 ttgatctcag ttttgtcatc accccaacca gccattcgca cataaccgcc caagggtaac
    28981 atcctcaagg tataaagagt tcctccttgg tcaacatgag aaaaaatttt agggcccatt
    29041 ccaatggcaa attctcttac tagaatgcct gattttttag caaaatagaa atgtccaaat
    29101 tcatggacaa tcactaaaat accaaaaata ataataaagg ttattattcc taacatagat
    29161 ttcctttcag tctattgctt ttttgagttc tgtcgcgttg ttgcgactcc aaacattacc
    29221 tcttaagatt agaataaacc aaagagatgc atgatgggaa aaacaaaaat catggaatca
    29281 aagcggtcta aaataccacc atgtcccggg ataagcttac ctgaatcttt gacaccaaag
    29341 tgacgtttga gagcactttc aaccaaatca ccaaattggg caaaaatgga aaaaagagcg
    29401 accaatacca acatggtcaa aaagtgatgt ggcgcataaa cagacctatc aatcaccata
    29461 aagataaaag acacaaggac tgcacaagcg atccctccta aactaccttc aatggttttg
    29521 tttggagaaa ctgtaggcaa tagtttgcgt ctgccaaact gacgcccaat cagatatgct
    29581 ccaatatcag tggcccaaac gataaataag gccaaaaaga ccttgtcaat tcctgacagg
    29641 cgagcattga tcaaatgttg aaaaccaatc ccaacataaa aactagttgc aatcggaaag
    29701 gctgcatcat caaatgaata agctctgcta tttaaaacag tacctgccaa aatgaaaaaa
    29761 accattaagc tgtaaaaagc aacattcgca tcaattggta aaaaagtgag gtaatggtcc
    29821 atcggaactg ccaatacaaa ggcggctaac atggcaaaaa caccttcaaa agaaaagact
    29881 tctaaccttt tcattttcag taattctgat accccaatca tggccaaaac gcctacaaat
    29941 aattggaatg gtaggtttcc tattatgaga aaaggtagga aaatagctac tgctactccc
    30001 ccccaaacaa cacgttcttt cataactatc tccttttaga ccttgccaaa acgacgttgg
    30061 cggtggttat aatccgcaat ggcctttagt agctctgcct ttttaaaatc aggccacaag
    30121 actggtgtga aataaaattc actgtaagct gattgccaag gcaagaaatt gctcaaacgc
    30181 agctcaccac tggtgcgaat aatcaaatcg gggtctcggt agagatatgg cagatggtct
    30241 gtcatcaagt aattagcaat caaatcctcc gtaatatctc ctggatttaa tttggcatct
    30301 aagacatctt gagcaataaa gcgaacagca ctggtaatct cagcacgtcc tccataattg
    30361 agagcaaaat tcaaaattaa acccgtatta cgttttgttt tgtcgatggc tgcatttagc
    30421 gctgccaagg tatcttcagg taaacgacta gtctctccaa tcatttgaat ctttacattg
    30481 ttctcatgaa gcacaggcac atatttgtca aagaaggtta ctggtaaatt catgataaaa
    30541 gacacctcat cttgaggtct ggaccagttt tctgtcgaaa aggcatagac tgtcagaact
    30601 ttaaccccca actcagatgc agtaatcgtt acttcttgta aagcatccat cccagcttta
    30661 tgcccaaaca ctctaggttt tagtcttttt ttggcccaac gtccattacc atccatgata
    30721 attccaatat gctttgggat actccccaaa actttttttg ttgatttcgc ttttaatcca
    30781 aacatgtttt ttccttattg ataagcatac ttatctcata ttttatcata tttcacgcct
    30841 tttggcttgt tttttatcaa ttagatctta tcttcacaaa atgttcatct atcctctgat
    30901 agatgtgtca gcttttcatg aacctgttag catcagaata actgtttcaa tccagggatc
    30961 aaaacaaaaa agtttggcat atttcatcca aactttcccc gccttaatgg ctttcaatgg
    31021 cactgtcagt gtcagtgcta tctactgtct caacagcagg ctcattaaca acactggttt
    31081 cttctgtttc aaccagtgtc gtttctgtgg tagcttttgt tacaatacgc ttaatagctg
    31141 atagctcaaa tgtcaaaaag acgccatcca catctaacac aatcttttta gctgttgtat
    31201 cgacttcgtc aacaatcgca aacatgccac caatcgttac gacttcgtct cctttttcaa
    31261 tagcgtttaa ctgattttgg cgttcttgcg cttgtttttt ttgttgacgt tgcataaacc
    31321 aaatcaatcc aagcatcact acaaacatta aaattgttgg aaatcccata atgtctcctt
    31381 tttcttctat aaaagtcatg tcttactata tcaaaaatca agcggtatag caagactaga
    31441 cgttacctga tttcttcttt aaacgagacg ctaagcgcag tgctttactc acgagtcaaa
    31501 aaagctaaga tgtcatctgg tgtcaaactt gaatcacata cagccctttg gtgctgatcg
    31561 taactaacta gcaaagctgg aactgtcact aattggtagt tctcacgaaa ggcagcaagc
    31621 tcagcagcat cagcagcgtt ttcgctatcc acaaaataga cttctttttg attatctgtt
    31681 gccacttgtg ccaacttagg agcaaagcgg cgacaataag gacagctcga acgtcctaaa
    31741 aagacaatca tgtctttgcc actagcaata gctgatgtga cttctgcaat actcgacggt
    31801 ataaaattcg caacgatttc ttcaaatgtc atggatgtct ccttctcttt tggtctcatg
    31861 atacactaaa gtagcaattt tctcaatcac aaggtctttt cacagctcag agcttttata
    31921 gccgtcaaaa agaatccgca aacggcaatc tttttgtctc tattaacacc accctaatct
    31981 ttttggcgct tcatcaacag gctaatagcc aataaaagca gagctactcc agctgctaat
    32041 aagccttttg aggaggcttc acccgttttt gggagctttt ggtttgtttt agctcttttt
    32101 ggcctagtca ttaatgattt gtctaggtct tttgagccat ctggtgtttt ggtttgatga
    32161 ttgcctgtct gactcttttg ctgagaggga ttagccgttt ttgaggatac tttgaggacc
    32221 agggcagtta ggccatcaat agtcaagcct tctttggtaa actggactcc cttaggctta
    32281 gcaatggcag taacaccagc ttgctcagca tcagcaagca cttgggctcc tagcaaatag
    32341 cggtaggctt gaggtaaaac taccttgcgg gtcttgttgt ctgcattgac aaagacagca
    32401 tagcgatctc catttgatgc caccgtttgg taacccatga tgaggtcctc ttgttgaata
    32461 ccatcttgtc ctgcttgggt gatcaaggtc acatcccgat ctacctcagc tttggttgct
    32521 tttgtaaagg catctgttga gcgacgcaac gcaattagtc cctgtgtata ggcttttgtt
    32581 tggttgctaa tcgggtgagc tatggaatct gttgccttgg cccagtcaaa atgattgacc
    32641 gcatcagacg aatcataaga atcgtggatg aagtaagggt atttcgctac agcatcaatc
    32701 agagtcgcct tatttggcac cttatcatca gacacctttg tcttgtagtc gggatttaga
    32761 agctgcttgg ttcgtccata ttcctgacca gaatggataa aggcagtccc ttgagcagtt
    32821 aaaatcatgg tatttcctag acgaatacgc ttgtgaatct cttcttcagc cactttagga
    32881 tccttattga tggatttggc aatgacatca tgtaaggtca ggttgtcatg ggctgcaata
    32941 tactgcacta catctcctgg ggcatctgct tcaaagttac taggctgtgc tttgatcgtt
    33001 ttgaacagtc cttctagatt ttttgcgcca ccagtaataa aggctgctgt gccttcattt
    33061 gggaaacctg acttgagggt atttctgata tcatcagaga aaacaccgac cgtattggtt
    33121 gctttcatcc aatcttgatc tgccgcaatt tcttttttcc cctcatcacc ttggtaggta
    33181 cgccagcctt cgccaatcat aatggtattt ggattaatgg ctttggctgc cttaaaggct
    33241 tgctcaatag ctgccgcatc atggtcaccc atcatgtcga aacgaaaacc atctaccttg
    33301 aattcacgag tcagataagt aatcgaatcc accaagatac gacgactcat ggcatgtgtc
    33361 gttcctagac gacctccgcc aaaactctct ctagctgtac catcagcatt cataaaatga
    33421 tagtagttgg gttccaaatc ttcaaagaga taggttctag ccgtgtggtt ataaaccaca
    33481 tcaaaaataa cacccatacc acgtttgtga atctcattga caaggttttt aagctctgcg
    33541 atacgtaaag cagggtcatt aggatttgcc gaatacatgc cagaaagggc aaagtagtgt
    33601 tgtgggtcat aaccccagtt gtaattattg tctgaagacg tgtaggctgt tgagcggctc
    33661 ttgtccagct cattggcata aaaataactc aaaaccggta gcaattgaac gtgggtaacc
    33721 cccaagtctt tgagatagtc tagctgttca acaaaagctg aaaaagtccc ataagggtgt
    33781 gttaacttac cttctagagc cttatctgac gtaaaatctc gcacatgtgc ttcatagata
    33841 atagcgtctt cacgcttttt aaagttgtta attttggcaa aatcaaggcc tgttggtcct
    33901 agtttgcttg gatcaataaa ggcagctttt gctgttttga tgtcatcagt agcagtcgca
    33961 tcattccagg cagcgagaga tttggcgtaa ggatccaaaa ccatgacttt ttcctgaccg
    34021 cgcgtgattt cataaagata ataatagcct gtgtaatcac taatgccctt gacgctgtca
    34081 gaagttagat gagctctcca aacacccttg tccgacttgg tcaaatcagc ttgaccaaga
    34141 acctttgtct gatcttgttt atcgtaaacg acaaccttaa cactgtcagc acttggagac
    34201 catagcgcta aatcaacaga accatcctta gctagggtag ctccaagttc accatcgtaa
    34261 gcatagagtt tatcttttaa ttgccaggat tggcgagcga cttggctaac ttctccaaat
    34321 gtaacagtgt agactgcacc ttgcgcctta aaatcaccct ttaatgtcat tacagacgta
    34381 tccttgtcaa gtgtcaattc atcaattgca acagttttcc ctgctttgtc agtgatcttg
    34441 atgttttgtt tcaccgcatc ttcatcaagc ccatctaagg tcgtaaaaat ggctttaatc
    34501 tcatttggcg tggtttgttc agctcctttg agactaacct gatcaatata ataaggattg
    34561 ttgtagactt ttgggtcagt gtctttgaca aaaacctgag tatgattgtc taactcttta
    34621 aacagataat cttttggttg cactttaata gcatctcctg tcttactttt atcaaggatt
    34681 aaaaatccga tttcgttagc tccttctttt aaggggacat caacataagc tccataaggc
    34741 cctttatgtg acaagtcaag tccatttggc cagtcggtcg ttggggtttt gacatcttta
    34801 aaggtccaga cagctaagtt atcgtagtgt cccgattgat tgtggtagtt gattcgaagg
    34861 taaccttttt tcaaaggtcg ataagcgtgt gcatggtaat tctcgtctat ccaaacttca
    34921 ttcatttttg gcgtgagaag cgagatgtgc tggtcctttg aaagatgctc tccagcttta
    34981 ttattaatga gataagacac ttgctggcgg tgtttagctg ctagtggcac atctagataa
    35041 tagccatagt catctttttt cgcctttgtc atggtgatag caccatttgg ccaatccttt
    35101 gaaggttgat ccacatctcc ccacacccaa agtcccaagc ttcctagcga ctcaccagct
    35161 ggcaatgttt taaaatgcat acgcaaatga tgctcagcaa tactagcagg attagctgac
    35221 gagctgtcta aaactggttt agcctcctta ctagacgatt gaaaaccatc accagattta
    35281 gcattgccat ctccttgttg agcttcatcg cgggctagtc caacaatggt tttagcagca
    35341 acttgatgtg ttaagatact aggggtgcac cctaacatga cagtcccagt tgcagcgatt
    35401 acaaaaccta tcccccactt ttttaacaga tactgatagc gctttgatcc ttggttgact
    35461 ttctttttca taagacctcc atttagtata acattcaaac aagactatct tatcacattg
    35521 cgcaagcgct tgcaagacat gccgcacttc aacttagaac acagctcaaa caatgactat
    35581 caccccttaa taggatctca aaaatcaaac agccaaaacg agtttccgct cgcttggctg
    35641 ttgggtaaac tcaaatcaca tacaactgct aggccttcag tttgagacaa aaagcatccc
    35701 aaggttgtag gtgcttggtc tctaacactt tgtccacatc tgtattggtg atgaccactt
    35761 ctgcacctgc taaatctttg gcaaagactt gctcttgatc agacacattg acaacaatca
    35821 catatgtctc ttctccaagc tgacgctgat aagcaaacac tttatcagct gttggcaaga
    35881 ggtgataatc cgcttcaact agccagtctt gctccttacg caaggcgatt agttgttggt
    35941 aggtgtaaaa aatagagtct ggatcagcaa gggcatcggc cacattgatg tcttggtagt
    36001 tggggttgac cggcaaccaa gtctcttggg ctttagaaaa gccagcatgt gtgtccttag
    36061 accactgcat tggcgttcta gcattgtctc gtcctacctt acggatgcta tccatcacac
    36121 gcgcagctga cactccattt cccatagctt ccttagcata gttaagggac tcaatatcat
    36181 taacctcggt taagtcttta aaaggataat tggtcatgcc aatctcttct ccttggtaaa
    36241 tgtaaggggt tcctcgcatc aaatgcagta aaatagctaa agccttggct gatttttctc
    36301 ggtaagtgct gtcattgccc caaatggata agacccgtgg caagtcatgg ttattccaaa
    36361 aaagcgaatt ccagccttct cccaacttta attctgtctg ccacttgcta aaaatagctt
    36421 tcagagcagg cacgtctaat tctttggcat aatcccactt gggagcgtca ggtttatgtt
    36481 gaagacccac atgctcaaat tggaagacca tggataactc tttgttttca gggcgagaat
    36541 actggcgagc aatttcaggc gttgctcccc aagtctcacc gactgtcatc acgtcatggt
    36601 tgccaaaggt tgcttgattc atttctttga ggtaatcgtg taagcgcggc ccgttacccg
    36661 tgatctccaa gtcaggaacc ttcccaatca ggtcaatcac atccatacga aaaccaccga
    36721 tgcctttggc aatccagaaa ttcatcatat cgtaaatttt atgacgcaaa gcttcattct
    36781 cccagttgag atctggctga cgtttgctaa aaaggtgcaa ataatattga cctgatgcat
    36841 tatcatattc ccaagctgag ccactaaaaa tagaagtcaa atcgttaggt tcatctcgcc
    36901 aaatataaaa atcacgctca ggactattag ggttttccct agcttcaaca aaccaagcat
    36961 gctcatcaga cgtatggttg accaccaagt ccatgataat cttaatgcct cgctcattag
    37021 ctgctgctaa gagctcatcc atatctgcca tgtctccaaa aacatcagca atcgcttcat
    37081 agtcagagat gtcataacca ttgtcatcca ttggactttg gtaaacagga gataaccaaa
    37141 tggctgtaat gccaagtttt tgcaagtagt ccagttgact ggtaatgcct tttaaatcac
    37201 caataccatt gccattggtg tctttaaagg atctcgggta aatctgataa atcgtagctt
    37261 tatgccacca gtgtttcttc attattcttc tcctattaat cattcattca tcttttattt
    37321 taacgttttt tctctttttg tgcaatcgtt tgcgcaaatg acatgataaa aaggctggaa
    37381 cccaaccttt ttatgaatat cgcttatacc ttaacgaata gcttgttcgg tatctctgtc
    37441 aaagaaatgc cccttagcca cattaaaagt caagctaact tgctcccctg gactatggaa
    37501 atcacgcgca tctacacgag aagcaaactc agtttgtcca agcttgacat agagcatcgt
    37561 ctcagagccc agtaactcag aaaccaaaac ttctgctgtg acagttgcac ttggataagt
    37621 atcatgcacg atttgtctgc tagaaatgtc ttctggacga ataccaaaag tgactttttt
    37681 ccccttgtaa ccagccgctt caagcatttt tgcttgtcct tcaggaatcg caatagcaag
    37741 accatcttca ctcacaatgc ggccatcttt aacttccact tcgaagaagt tcatggcagg
    37801 gcttccgata aagccggcaa cgaatttatt agctggaagg ctgtacaatt cttgtggcga
    37861 acccacttgt tcaattttac caatcgttcc attaccctga gggtttttag tggcactcat
    37921 gataacgata cggtctgcca aggtcatggc ttctgtctgg tcatgggtaa cgtaaatagt
    37981 tgttgagcca atcctacggt gaatctttgc gatttcagca cgcatggaga cacgaagctt
    38041 cgcatccaag tttgacaaag gctcatccat caagaacact ttagcatcac ggacaatagc
    38101 acgccccatg gccacacgct gacgttgacc accagacagg tcagcaggtt tacgctctaa
    38161 aaattcggtc aaaccaagaa tctgcgcagc ttccttgaca cgacggtcaa tatcatcttt
    38221 tttgtactta cgtaatttca aaccaaaggc catgttatca tacacagtca tgtgtgggta
    38281 aagggcgtag ttttgaaaga ccatggcaat atcacggtct ttaggagact tgtcattcac
    38341 cacttcgcca ccgattttta actctccttc agaaatgtcc tcaagacctg caatcatacg
    38401 aagagtagtc gatttaccac atcctgaagg tccaacaaag acgataaatt ctttatcttt
    38461 gatgtccaag tcaaaatctt caacggcata atgagtagta ttggggtatt ttttatagat
    38521 gtgatttaga tttaattcaa ccatgtgtaa ctcctattta gccagcaaac gttctagctg
    38581 actgcaaagc tagaaaccgc tgatatcttt tatatgatgc ttttattata acaaaaatag
    38641 cgctttcaaa acagagacaa agcgaccaaa cttctttggg caacttgcac aatagcaggg
    38701 ctagtcaagt cgtaagggac cataaaccaa gaaaactatg taaacacagg ccggtcacag
    38761 gagccaaaac aagctgaacg atctatcatt ctccttatca gctacagtcc aggcagcaaa
    38821 aaagaaagcc ctgtggcttt cctaatattt caacaaaaag agatgagcaa aggctaaatc
    38881 atccaattgc ttcaagtgta gtcctgattg ctgagcaaat ttatcaagtt tgtattgcaa
    38941 ggagttacgg tgaatataca agcgctgcgc tgtctgcacc agattgccgt gttctgacca
    39001 tagcgcgtga accacatcgg ccatatcact ttgaggactt aggaattgtt gaaactgacg
    39061 cgtcaatgcc aaaaaagtgt ggtgcgacag caaagaccac agcatcagtc ctgaaaatgt
    39121 taataatttt ctgcctgatt gttgccttaa ataggccgtt aacagctgac attcctcctc
    39181 aaaacactct ctcaatgtac ccgcagctac ttgataccag gcatttccaa caaacatggt
    39241 taaggccaaa ccaaaatcac tttctagcgt tggcaagaga tcttcaagcc agcgcaagac
    39301 ctttattgag actgcttgtc ggcataaaaa tgccgtttgg gttgcactaa taggcaaaat
    39361 gacctcaagc cctgcaatca tctgttggag caactcaact aagtcagctg gtaaagcctg
    39421 ctgatgatta aggtaaatga actgagaagt ctcaaacagc tgagggggat tgccctgatg
    39481 atcaagcaaa taccgctgcc aaggtgaccc tagcggctgc aagacctcac actgatccag
    39541 tcccacctca agtaataagc gctctttttc ggataaacat gatttgggaa aatgcacata
    39601 ttggtcccct tctttgacac tcacccactc tttatcccct aacggatgag ggcctacttg
    39661 catctctgga aaatagtctt ttagctccat agccattcct ttcatgacgg tctttaaacc
    39721 attataacac atgactcttt atcacacagt tcggtttgtt gtcagcatta ttttgtattt
    39781 tctgactttt ttaatcatta aagctaaata atggttattc atttttagca agaacattca
    39841 atcaaatagc tatttatcgg aagattaatt tatgtttata ctaaaatagg tattatttct
    39901 cttttttcat ta
//
LOCUS       DAUDYX010000013        37970 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.013, whole genome shotgun sequence.
ACCESSION   DAUDYX010000013 DAUDYX010000000
VERSION     DAUDYX010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37970
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.013"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            512..730
                     /locus_tag="VHD02_000638"
     CDS             512..730
                     /locus_tag="VHD02_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ5497270.1"
                     /translation="MYPRIRNLREDNDFTQKFVANLLSFSHANYAKIERGEVALMADV
                     LVQFSKLYNVSIDYLLGQTDYPYRFHNK"
     gene            complement(801..944)
                     /locus_tag="VHD02_000639"
     CDS             complement(801..944)
                     /locus_tag="VHD02_000639"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497271.1"
                     /translation="MGISPTGLLRWQPERSEVSLSLFVVMADREPLYFIANNYRSYHG
                     VSH"
     gene            1031..1251
                     /locus_tag="VHD02_000640"
                     /pseudo
     CDS             1031..1251
                     /locus_tag="VHD02_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
     gene            1407..1628
                     /locus_tag="VHD02_000641"
     CDS             1407..1628
                     /locus_tag="VHD02_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEQ5497272.1"
                     /translation="MKEAYDELVRIRYEFSNNLSIENDNISFENYMNTIYLRAYKQKV
                     QAVTYEIALPHHKLFIQYFGSKHLKGITA"
     gene            1953..2336
                     /locus_tag="VHD02_000642"
     CDS             1953..2336
                     /locus_tag="VHD02_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEQ5497273.1"
                     /translation="MKVHTTLEKDENGNWYRKDQTKTPAGERLIELDDVTIVVLENWR
                     RNQVVNTDTDFIISRFGEPFCKSTICRVIKHKAQEVGVPVITGKGLRHSHASYLINVL
                     KKDILYVAKCMGHADKSTTLNTYSH"
     gene            2599..3834
                     /locus_tag="VHD02_000643"
     CDS             2599..3834
                     /locus_tag="VHD02_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEQ5497274.1"
                     /translation="MIKRWVILFFMVLTCSGFGKTVLAADFQVGAEHAIVVEADSGRV
                     LYEKDAKTPDAIASLTKLVTAYLVLDKVKSGQLQLSDQVNLSDYAFELTKDRSLSNVP
                     FDKKTYSVQDLLTATLVASSNSAAIALAEKVAGSEPHFVNQMREQLSHWGITSGKILN
                     ASGLPNEVLKDHRYPGSTLEEENTLSAQDVAVVTMHLLEDFPEILEITKQTEVNFAGN
                     SIKSFNQLLPGMAKGRAGVDGLKTGTTDLAGHCLVVTSIENGMRVITVILNADGSDKN
                     QNTRFEQANRLLDYVARTYCCRKILKKGSLVSERSLPIQDGQVKELPVSVAEDVTIIL
                     QQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQS
                     LKKASDLQLWWRDFLEKRR"
     gene            complement(3951..4913)
                     /locus_tag="VHD02_000644"
     CDS             complement(3951..4913)
                     /locus_tag="VHD02_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEQ5497275.1"
                     /translation="MNNRHKRRQKRKAFTLINLILLFACILGLTLIFYLYQGSNKFGL
                     SDVSQFVPKISLFHHKKTAKKETTKLKKTHFDSSKSQKKAHSKLTWTKQETPVKIPIL
                     MYHAIHVMSPEETANANLIVNPDLFDQQLQKMKDEGYYFLSPEEVYRALSNNELPAKK
                     VVWLTFDDSMIDFYNVAYPILKKYDAKATNNVITGLTEIGSAANLTLKQMKEMKQFGM
                     SFQDHTVNHPDLEQTSPDVQTTEMKDSKDYLDKQLNQNTIAIAYPSGRYNDTTLQIAA
                     RLNYKLGVTTNEGIASAANGLLSLNRIRILPNMSPENLLQTMEP"
     gene            5245..6522
                     /locus_tag="VHD02_000645"
     CDS             5245..6522
                     /locus_tag="VHD02_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEQ5497276.1"
                     /translation="MTYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHI
                     VGTNGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFKERISINGRMISEKDLVIAANRI
                     RPLTERLVQETDFGEVTEFEVITLIMFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAM
                     VVVCPSIGLDHQAILGETYADIAAQKAGVLEGGETLVFAVENPSAREVFLTKAEQVGA
                     SIWEWQEQFQMAENASGYRFTSPLGVISDIHIAMPGHHQVSNAALAIMTCLTLKDRYP
                     RLTSDHIREGLANSLWLGRTELLAPNLMIDGAHNNESVAALVAVLKNNYNDKKLHILF
                     GAIDTKPIADMLVALEQIGDLQVTGFHYPNAYPLEKYPERFGRVADFKDFLALRKHAK
                     ADDFFVITGSLYFISEIRQYWKKHIEKNVLLTH"
     gene            6557..7141
                     /gene="folE"
                     /locus_tag="VHD02_000646"
     CDS             6557..7141
                     /gene="folE"
                     /locus_tag="VHD02_000646"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEQ5497277.1"
                     /translation="MKRERLMSINKEKAEAAIYQFLEAIGENPNREGLLDTPKRVAKM
                     YAEMFLGLGKDPKEEFTAVFKEQHEDVVIVKDISFYSICEHHLVPFYGKAHIAYLPSD
                     GRVTGLSKLARAVEVASKRPQLQERLTSQIADALVEALNPKGTLVMVEAEHMCMTMRG
                     IKKPGSKTITTTARGLYKESRAERQEVISLMTKD"
     gene            7150..7950
                     /gene="folP"
                     /locus_tag="VHD02_000647"
     CDS             7150..7950
                     /gene="folP"
                     /locus_tag="VHD02_000647"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEQ5497278.1"
                     /translation="MKIGKFVIEGNAAIMGILNVTPDSFSDGGSYTTVQKALDHVEQM
                     IADGAKIIDVGGESTRPGCQFVSATDEIDRVVPVIKAIKENYDILISIDTYKTETARA
                     ALEAGADILNDVWAGLYDGQMFALAAEYDAPIILMHNQDEEVYQEVTQDVCDFLGNRA
                     QAALDAGVPKNNIWVDPGFGFAKSVQQNTELLKGLDRVCHLGYPVLFGISRKRVVDAL
                     LGGNTKAKERDGATAALSAYALGKGCQIVRVHDVKANQDIVAVLSQLM"
     gene            7957..8316
                     /gene="folB"
                     /locus_tag="VHD02_000648"
     CDS             7957..8316
                     /gene="folB"
                     /locus_tag="VHD02_000648"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEQ5497279.1"
                     /translation="MDKIVLEGCRFYGYHGAFKEEQTLGQIFLVDLELSVDLQAASLS
                     DQLTDTVHYGMVFDSVRQLVEGEKFILIERLAGAICEQLFNEFPPIEAIKVAIKKENP
                     PIAGHYKAVGIELERQR"
     gene            8313..8813
                     /gene="folK"
                     /locus_tag="VHD02_000649"
     CDS             8313..8813
                     /gene="folK"
                     /locus_tag="VHD02_000649"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEQ5497280.1"
                     /translation="MTIVYLSLGTNMGDRAAYLQKALEALADLPQTRLLAQSSIYETT
                     AWGKTGQADFLNMACQLDTQLTAADFLKETQAIEQSLGRVRHEKWGSRTIDIDILLFG
                     EEVYDTKELKVPHPYMTERAFVLIPLLELQPDLKLPPNHKFLRDYLAALDQSDITLFS
                     AQQTEF"
     gene            8963..9850
                     /gene="murB"
                     /locus_tag="VHD02_000650"
     CDS             8963..9850
                     /gene="murB"
                     /locus_tag="VHD02_000650"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEQ5497281.1"
                     /translation="MITELHGIDIRENEPLKHYTYTKVGGPADFLAFPRNHYELSRIV
                     AYANKENMPWLVLGNASNLIVRDGGIRGFVIMFDKLNAVHLNGYTLKAEAGANLIETT
                     KIAKFHSLTGFEFACGIPGSIGGAVFMNAGAYGGEISHIFLSAKVLTPSGEIKTISAR
                     DMAFGYRHSAIQETGDIVISAKFALKPGNYDTISQEMNRLNHLRQLKQPLEFPSCGSV
                     FKRPPGHFAGQLIMEANLKGHRIGGVEVSEKHAGFMINVADGTAKDYEDLIAYVIETV
                     ENHSGVKLEPEVRIIGENL"
     gene            9896..11050
                     /locus_tag="VHD02_000651"
     CDS             9896..11050
                     /locus_tag="VHD02_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5497282.1"
                     /translation="MTKPIITFNNVSKTFEDSGTQVLKNINFDLEEGKFYTLLGASGS
                     GKSTILNIMAGLLDASSGDIYLDGERINDLPINKRDIHTVFQNYALFPHMTVFENVAF
                     ALKLKKVDKKEIAKRVKETLKMVQLEGYENRSIQKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVANFIGESNIINGTMIEDYLVSFNGKEFESVDGGMRPNEPVEV
                     VIRPEDLQITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIEGEVIG
                     LDFTPEDLHIMRLNETEEEFDARIEEYVEMDEPEDGLINAIEEERNEENL"
     gene            11034..11828
                     /locus_tag="VHD02_000652"
     CDS             11034..11828
                     /locus_tag="VHD02_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ5497283.1"
                     /translation="MKKTSSLFSIPYFLWILFFVVAPVTLLFYKSFFDIEGRVTLANY
                     ETFFSSWTYLRMSVNSILYAGIITLVTLLISYPTALFLTRLKHKQLWLMLIILPTWVN
                     LLLKAYAFMGIFGQQGGINSFLTFMGIGPQQILFTDFSFIFVASYIELPFMMLPIFNA
                     LDDIDHNVINASRDLGASEFQAFSKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLTTQNWGMGSTIGVVLILTMVAIMWLTKEKSK"
     gene            11825..12601
                     /locus_tag="VHD02_000653"
     CDS             11825..12601
                     /locus_tag="VHD02_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ5497284.1"
                     /translation="MKKFANLYLASVFVLLYIPIFYLIFYSFNKGGDMNGFTGFTLEH
                     YQTMFEDSRLMTILLQTFVLAFSSALLATIIGTFGAIFIHHVRGKYQNAMLSANNVLM
                     VSPDVMIGASFLILFTSLKFQLGMSSVLLSHIAFSIPIVVLMVLPRLKEMNQDMVNAA
                     YDLGANYFQMLKEVMLPYLTPGIIAGYFMAFTYSLDDFAVTFFLTGNGFTTLSVEIYS
                     RARQGISLDINALSTIVFFFSILLVIGYYYMSQDKEEKHA"
     gene            12594..13667
                     /locus_tag="VHD02_000654"
     CDS             12594..13667
                     /locus_tag="VHD02_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEQ5497285.1"
                     /translation="MRKLYSFLAGVLGVIVILTSLSFILQKKSGSGSQSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMYTKIKQGGTTYDIAVPSDYTIDKMIKEN
                     LLNKLDKSKLVGMDNIGKEFLGKSFDPQNDYSLPYFWGTVGIVYNDQLVDKAPMHWED
                     LWRPEYKNSIMLIDGAREMLGVGLTTFGYSVNSKNLEQLQAAERKLQQLTPNVKAIVA
                     DEMKGYMIQGDAAIGITFSGEASEMLDSNEHLHYIVPSEGSNLWFDNLVLPKTMKHEK
                     EAYAFLNFINRPENAAQNAAYIGYATPNKKAKALLPDEIKNDPAFYPTDDIIKKLEVY
                     DNLGSRWLGIYNDLYLQFKMYRK"
     gene            complement(13722..14387)
                     /locus_tag="VHD02_000655"
     CDS             complement(13722..14387)
                     /locus_tag="VHD02_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEQ5497286.1"
                     /translation="MNVLIIEDDPMVDFIHRNYLEKLNLFDRIISSDSMKAVQSILTD
                     YAIDLILLDIHITDGNGIQFLEKWRAQHIPCEVIIISAANDGNIIRDGFHLGIIDYLI
                     KPFTFERFQESIQQFVTHREHLANQQLEQAQIDQLKCLTSKKDTKNKQLLEKGLSEST
                     FQWIMENIKAFDQPFTIQELASACHLSHVSVRKYIAYLEENKQLNSQQIFTKVGRPYR
                     VYW"
     gene            complement(14368..15909)
                     /locus_tag="VHD02_000656"
     CDS             complement(14368..15909)
                     /locus_tag="VHD02_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ5497287.1"
                     /translation="MKKPLRLWASLSLILVSMIVVTTSLFYGIMLHDTHQSIKNQETH
                     LLTSTGKMLASHQAIKELLLNNQPNAKTTAYTNSIASIYNLDYVVVMNMKGIRLTHPN
                     PKNIGKPFQGGDEEAVLAGKKVISTAKGTLGKSLRYLVPVFDGDKQIGAIAVGIKLTT
                     LNDVALTSKRNYTLSLLLCLLISLLVTSFISFRLKRQLHQLEPSEIYQLFEERNAMLD
                     QIEAAVFVVDKAGILQLCNQAGQKLIARKCQLGKPTGNSFNHLFPDFPKLSLQEGHEQ
                     LFRYGEEDYLLAISPICVKNDHRGHIIFMREAVKAIDTLDQLAYTTAYASALQAQTHK
                     FMNQLHVIYGLVDIAYYDQLKIYLDSILEPENEILTSLSVLVKEPLLASFLIGEQEKY
                     QELNVHLKIDVLSEIPHSATKNQLNNGLMIYRFIHTNLLTTLRPKSLVLSIQHDQNHL
                     ISHYTLTDNWIDLERVQPIFDLPYFQQLLTDTNSQFHQHQTVTELQFSVTTPYVGGQH
                     ERFNH"
     gene            16070..17401
                     /locus_tag="VHD02_000657"
     CDS             16070..17401
                     /locus_tag="VHD02_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEQ5497288.1"
                     /translation="METISKKMPQKDLSEHSKAWQNRRIGSVPLPVYLVLATLILVTG
                     WLQQLPVNMLGGFAVILTLGWLLGTIGATIPGLKHFGGPAILSLLVPSILVFFNLLNP
                     NVLEATNVLMKQANFLYFYIACLVCGSILGMNRKILIQGLFRMIIPMLLGMVCAMGVG
                     TLVGVILGLDWQHTLFYVVTPVLAGGIGEGILPLSLGYSAITGVGSEQLVAQLIPATI
                     IGNFFAILCTALLNRFGEKHPSYSGQGQLVKIGHSENMSDALKDNSGALDVKLMGAGV
                     LTACSLFIAGGLLQHLTGFPGPVLMIILAAFLKYLNVIPQETQNGAKQLYKFISSNFT
                     FPLMAGLGLLYIPLKDVVATLSWQYFIVVISVVLTVVSVGFFVSRFLNMNPVEAAIIS
                     ACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRIIFS"
     gene            17433..18599
                     /locus_tag="VHD02_000658"
     CDS             17433..18599
                     /locus_tag="VHD02_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEQ5497289.1"
                     /translation="MENQLGQLALEQAKTFGGKLEVQPKVDIKNKHDLSIAYTPGVAS
                     VSSAIAKDKTLAYDLTTKKNTVAVISDGTAVLGLGDIGPEAAMPVMEGKAALFKAFAG
                     VDAIPIVLDTKDTEEIISIVKALAPTFGGINLEDISAPRCFEIEQRLIKECHIPVFHD
                     DQHGTAIVVLAAIFNSLKLLKKSLDEVSIVVNGGGSAGLSITRKLLAAGATKVTVVDK
                     FGIINEQEAAQLAPHHLDIAKVTNREFKSGTLEDALEGADIFIGVSAPGVLKAEWISK
                     MAARPVIFAMANPIPEIYPDEALEAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAAKGIASLVPDDALSTTNIIPDAFKEGVAEIVAKSVRLVVLKS"
     gene            complement(18681..19730)
                     /locus_tag="VHD02_000659"
     CDS             complement(18681..19730)
                     /locus_tag="VHD02_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEQ5497290.1"
                     /translation="MKAATYLSTGNLQLIDKPKPVIIKPTDAIVQLVKTTICGTDLHI
                     LGGDVPACKEGTILGHEGIGIVKEVGDAVTNFKIGDKVIISCVTSCHTCYYCKRGLSS
                     HCEDGGWILGHLINGTQAEYVHIPHADGSLYHAPDTIDDEALVMLSDILPTSYEIGVL
                     PSHVKPGDNVCIVGAGPVGLAALLTVQFFSPANIIMVDLSQNRLEAAKTFGATHTICS
                     GSSEEVKAIIDDITNGRGVDISMECVGYPATFDICQKIISVGGHIANVGVHGKPVDFN
                     LDELWIKNITLNTGLVNANTTEMLLNVLKTGKIDATRLITHHFKLSEVEKAYETFKHA
                     GANNALKVIIDNDIS"
     gene            19966..20697
                     /gene="aphA"
                     /locus_tag="VHD02_000660"
     CDS             19966..20697
                     /gene="aphA"
                     /locus_tag="VHD02_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEQ5497291.1"
                     /translation="MKKEFTSILFTVSFCGIIALPVEASGPKVPYTQEGITAISNQAT
                     VKLISIADIASSLEGQKPITVSFDIDDTLLFTSQYFQYGKEYITPGSFDFLHKQKFWD
                     LVAKRGDQDSIPKEYAKQLIAMHQKRGDKIVFITGRTRGSMYKKGEIDKTAKSLAKDF
                     KLDKPIAINYTGDKAVKPYQYDKTYYIKKNGSQIHYGDSDEDINAAKEAGARPIRILR
                     APNSTNLPLPKAGGYGEEVLENSAY"
     gene            20875..22407
                     /locus_tag="VHD02_000661"
     CDS             20875..22407
                     /locus_tag="VHD02_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEQ5497292.1"
                     /translation="MENHKNEFTFSNKSIIAYVWRGVIVGIIAGVIVSLFRLLIEVTA
                     DWVIEWYRYAHINSLLLLPILSVSLLAVLFVGFLVKSDSDIKGSGIPHVEGELKGLMS
                     PDWWSVLWKKFLGGIMAISMGFMLGREGPSIQLGAMSAKGLAKFLKSSRLEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLIWITALVASLVANFISLNIFGLKPVLA
                     MSEAMPFLGLNQYWLLLLLGLFLGCLGYLYEIVILNFNKLYVILGSWLHLPAYFYGII
                     MVFLILPIGYYLPQLLGGGHGLILSLSNQQLPLMTIFFYFIIRFIVSMFSYGSGLPGG
                     IFLPILTLGALAGLLFGQIASQLGLLNQSFLSLFLILGMAGYFAAISKAPLTGMILVT
                     EMVGDLKPLMAIAVVTFVSYLVMDLLNGQPIYEAMLDKMAMKHPTNLVEPTLIELTVG
                     DKIAGKYVKELKLPENVLITTQIHHQKSQVVSGNTRLLSGATIFLVVNEADTGFVREV
                     LM"
     gene            22570..23184
                     /locus_tag="VHD02_000662"
     CDS             22570..23184
                     /locus_tag="VHD02_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEQ5497293.1"
                     /translation="MLEIVSEELRHYQEQKLIEYRNKNQLAPKGGIVFAGDSLIEFFP
                     LKKAFGSCLPIINRGIAGIDSQWLLRHFSVQITDLEPKHIFLLIGCNDIGLGYDKCHI
                     VKTIVELISQIRSHCVYSQIYLLSLLPVSNNPRYQKTVKIRTNAMIDAINKDLAMIPT
                     IEFINLNTCLKDEKGGLSDENTLDGLHLNFPAYAKLAEIIQAYI"
     gene            23343..24491
                     /locus_tag="VHD02_000663"
     CDS             23343..24491
                     /locus_tag="VHD02_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEQ5497294.1"
                     /translation="MIIKGVTMKFEKKQVFYLVLTFILCYGILANWRNGTAIVTTIYK
                     TSLPFFYGAAGAYIVNIVMSAYEKVYVYIFKDWSHVLKVKRGICLLLAYLTFFILITW
                     IISIVIPDLITSISTLTKFDTITIQEVVNNLEHNKLLARTIQYIGGDGKLTETIANYS
                     QQLLKQFLTVLTNILTSVTVIASAIINLFISFVFSLYVLASKEDLCRQGNTLVDTYTG
                     KYAKRIHYLLELLHQRFHGFFVSQTLEAMILGSLTASGMFILRLPFAGTIGVLVAFTA
                     LIPVIGASIGAAIGFILIMTQSMSQAIIFIIFLIILQQIEGNFIYPKVVGGSIGLPAM
                     WVLMAIAIGASLKGIVGMIIAVPLAATLYQVIKDNIQKRQAIQKKQVS"
     gene            24540..25220
                     /gene="radC"
                     /locus_tag="VHD02_000664"
     CDS             24540..25220
                     /gene="radC"
                     /locus_tag="VHD02_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEQ5497295.1"
                     /translation="MYSIKCDDNKAMPRERLMRLGAESLSNQELLAILLRTGNKEKHV
                     LELSSYLLSHLDSLADFKKMSLQELQHLAGIGKVKAIEIKAMIELVSRILATDKTLTD
                     SVLTSVQVAEKMMAALGDKKQEHLVVLYLDNQNRIIEEKTIFIGTVRRSLAEPREILY
                     YACKNMATSLIVIHNHPSGNIEPSSNDYCFTEKIKRSCEDLGIICLDHIIVSYKDYYS
                     FREKSTLF"
     gene            complement(25222..25917)
                     /locus_tag="VHD02_000665"
     CDS             complement(25222..25917)
                     /locus_tag="VHD02_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEQ5497296.1"
                     /translation="MTKPIIGITANQRLNMALDNLPWSYAPTGFVQAVTQSGGLPLLL
                     PIGDEAAAKTYVSMVDKIILIGGQNVDPKYYQEEKAAFDDDFSPERDTFELAIIKEAI
                     TLKKPILGICRGTQLMNVALGGNLNQHIDSHWQEAPSDFLSHEMIIEPDSILYPIYGH
                     KTLINSFHRQSLKTVAKDLKVIARDPRDGTIEAVISTNDAIPFLGVQWHPELLQGVRD
                     EDLQLFRLFVNDF"
     gene            complement(25927..26571)
                     /locus_tag="VHD02_000666"
     CDS             complement(25927..26571)
                     /locus_tag="VHD02_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEQ5497297.1"
                     /translation="MVIDKSIPKATAKRLSLYYRIFKRFHADQVEKASSKQIADAMGI
                     DSATVRRDFSYFGELGRRGFGYDVTKLMNFFADLLNDHSTTNVILVGCGNIGRALLHY
                     RFHDRNKMQIAMGFDTDDNALVGTKTADNIPVHGISSVKERIANTDIETAILTVPSIH
                     AQEVTDQLIEVGIKGILSFAPVHLQVPKGVIVQSVDLTSELQTLLYFMNQNHLD"
     gene            complement(26823..27170)
                     /locus_tag="VHD02_000667"
     CDS             complement(26823..27170)
                     /locus_tag="VHD02_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEQ5497298.1"
                     /translation="MAFEKEIKLKDCKYLYTISPNIKKYTLRDTTFSQTKVGHYQLIR
                     LLEKIPNSGDGFPLKITINKELTGFKLAITDQSGLRLVNIFKTKDNQILQDKFYFLMD
                     SLVEREIFSKTVV"
     gene            complement(27160..28287)
                     /locus_tag="VHD02_000668"
     CDS             complement(27160..28287)
                     /locus_tag="VHD02_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ5497299.1"
                     /translation="MTYFDNAATTPLSPNVIRAMTAAMQDNFGNPSSIHFYGRRANKI
                     LRECRQAIARNLGASEQQIIVTSGGTESNNMAIKGYALAHQAKGKHLITTTIEHHSVL
                     HTMAYLEERFGFEVTYLPCQNGQINLSDLKQALRDDTILVSIMYANNETGDLLPIKDI
                     GNLLKDHQAAFHVDAVQAVGKLKIIPSELGIDFLSASAHKFHGPKGCGFLYSNGQPID
                     PLLHGGDQEGKRRASTENMLGIIGMAQALTDAMTCLDQSTDHIISLRHHLISLLEGLP
                     YYINQGTHYLPHVLNIGFLGYQNTILLTQLDLAGIAVSTGSACTAGAVNPSHVLAAYY
                     GDDSSRLKESIRISFSDQNSIEDVNQLAQTLKNILGGTDGF"
     gene            complement(28284..29264)
                     /locus_tag="VHD02_000669"
     CDS             complement(28284..29264)
                     /locus_tag="VHD02_000669"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEQ5497300.1"
                     /translation="MTERYADKQIKLFSLTSNLPIAEKIAKAAGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVLPYFGYSR
                     QDRVAKPREPITAKLVANMLTKAGIDRVVTLDLHAVQVQGFFDIPVDNLFTVPLFAER
                     YSKLGLSGSDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREQGYIIGDVSG
                     KKAILIDDILNTGKTFAEAAKILERSGATDTYAVASHGLFAGGAAEVLETAPIKEIIV
                     TDSVKTKNRVPENVTYLSASDLIAEAIIRIHERRPLSPLFSYQPKGKNNA"
     gene            complement(29404..29982)
                     /locus_tag="VHD02_000670"
     CDS             complement(29404..29982)
                     /locus_tag="VHD02_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEQ5497301.1"
                     /translation="MTNLEIEYKTLLTKNEYNRLLSQMKHVTPVTQTNYYIDTKAFDL
                     KANKMSLRIRTFANSAELTLKVPEKVGNREYNVPLFLEQAKDMIKHGNLPESTALDII
                     ISKGIKPSALVTFGNLTTVRRETVIPIGKLALDYNLYANTKDYELELEVSDALQGKID
                     FDSFLSEHHIAFKYAKSKVARCINTLKKFNDK"
     gene            30070..30741
                     /locus_tag="VHD02_000671"
     CDS             30070..30741
                     /locus_tag="VHD02_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEQ5497302.1"
                     /translation="MTLDWEEFLDPYIQTVGELKIKLRGIRKQYRKQNRYSPIEFVTG
                     RVKSIESIKEKMILRGVIEENIAQDIQDIAGLRIMVQFVDDVEEVLALLRQRQDMTIV
                     YERDYIRNMKSSGYRSYHVVVEYPVDTIEGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YGGDFPEEIKKRLEVTAKIALELDEEMRKIREDIREAQLLFDPVTRNLSDGVGNSDDT
                     DELYR"
     gene            30716..31552
                     /locus_tag="VHD02_000672"
     CDS             30716..31552
                     /locus_tag="VHD02_000672"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEQ5497303.1"
                     /translation="MTQMNYTGKVKRVAIIANGKYQSKRVASKLFSVFKDDPDFYLSK
                     KNPDIVISIGGDGMLLSAFHMYEKELDKVRFVGIHTGHLGFYTDYRDFEVDKLIDNLR
                     KDKGEQISYPILKVAITLDDGRVVKARALNEATVKRIEKTMVADVIINHVKFESFRGD
                     GISVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVFRTLGSSIIIPKKDKIE
                     LVPKRLGIYTISIDNKTYQLKNVTKVEYFIDDEKIHFVSSPSHTSFWERVKDAFIGET
                     DS"
     gene            31549..32454
                     /locus_tag="VHD02_000673"
     CDS             31549..32454
                     /locus_tag="VHD02_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEQ5497304.1"
                     /translation="MRFEFVADKRIKVKTLLKSHDVSKGLLAKIKYKGGNILVNGIEQ
                     NAIYLLQVGDVVTIDIPNEEPFEKLEAIPFDLDIVHEDDHFLVINKPIGFASIPSAIH
                     SNTIANFIKAYYVDNHYLDQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQTRSIE
                     KRYFALVSGNGTLPDEGDIIAPIGRSKDSIITRAVDPMGKYAKTSYKVVARYSENVHL
                     VDIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRLDLGITRQALHCHYLNFKDPFTESD
                     CSYAIHLTDDFDSVIIGLQKKDRGY"
     gene            32458..33453
                     /gene="pta"
                     /locus_tag="VHD02_000674"
     CDS             32458..33453
                     /gene="pta"
                     /locus_tag="VHD02_000674"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEQ5497305.1"
                     /translation="MSIRSLFGGLREKILGKNMKIVFPEGNDERVVRAAARLKFEGLL
                     EPIILGQSEEVRNLLTKLGFADQDYTIINPNEYADFDKMKEAFVDIRKGKATLEDADK
                     MLRDVNYFGVMLVKMGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRE
                     NTSERYVFADCAINIDPTAQELAEIAVNTAETAKIFDIDPKIAMLSFSTKGSGKAPQV
                     DKVREATEIAKGLNPDLALDGELQFDAAFVPETAAIKAPDSAVAGQANTFVFPDLQSG
                     NIGYKIAQRLGMFDAIGPILQGLNKPVNDLSRGSSAEDIYKLAIITAAQAIESQG"
     gene            33579..34334
                     /locus_tag="VHD02_000675"
     CDS             33579..34334
                     /locus_tag="VHD02_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEQ5497306.1"
                     /translation="MGNIALVTGASAGFGRAIVEHLIADGIKVIGAARRLEKLKALET
                     SFGSDNFYPLQMDVTDTKAIDDALTSLPNQWQDITILVNNAGLALGLDKAYEADFENW
                     MTMINTNIVGLIYLTRQLLPHMVSKDAGIIINLGSTAGTIPYPGANIYGASKAFVKQF
                     SLNLRADLAGSHIRVTNIEPGLCEGTEFSKVRFNGDEARVKAIYQGAHAIQPQDIAST
                     VSWVIHQPPHVNVNRIELMPVSQSYGPQPVSRD"
     gene            complement(34528..35217)
                     /locus_tag="VHD02_000676"
     CDS             complement(34528..35217)
                     /locus_tag="VHD02_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HEQ5497307.1"
                     /translation="MKNAISILALLTPENQLEDTFIRELSHHFSHLFEVTILTSKANI
                     QSNQLSTFQGIFSFHEHDIDLPTLYFKTSQHGQGFLVTESVFDQATTVLLLSQYLTRF
                     YQKFDGYFLQYLPLQARLSDAKGNILVDNHAFNGSFLPTTDNEIEDWILAELRLSDSP
                     CKTFLLPSGSLDHIYMQHYQALKNPQGQLVGVLDTVQDIKPLLNQYLEETGQAIVGWS
                     DVTSGPSILDH"
     gene            35643..36371
                     /locus_tag="VHD02_000677"
     CDS             35643..36371
                     /locus_tag="VHD02_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5497308.1"
                     /translation="MIRFNNVSKTFGQTKVLQEQTFQINDREFFVLVGPSGSGKTTLL
                     KMINCLIEPSSGDILLNNVPQKELDLREMRLSIGYVLQQIALFPNLTVAENIAIIPEM
                     KQWSAEEIRQKTEELLDKVGLPAKDYLDRYPSDLSGGEQQRIGIVRAIISHPKILLMD
                     EPFSALDPISRKQLQELMLSLHKEFDMTIVFVTHDIDEAIKLGDRVAILNEGEIVQLD
                     RPEMIKTHPANAFVVNLFGGDEHA"
     gene            36364..37890
                     /locus_tag="VHD02_000678"
     CDS             36364..37890
                     /locus_tag="VHD02_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEQ5497309.1"
                     /translation="MPSLFVTFQNRFNEWLAALGEHLQISLLSLMIALLIGVPLAALL
                     SRSKRWSDIMLQVTGVFQTIPSLALLGLFIPLMGIGTLPAVTALVIYAIFPILQNTIT
                     GLNGIDPSLVEAGIAFGMTKWERLKTFEIPIAMPVIMSGVRTSAVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNANLILIGAISSALLAIIFNSLLQYLEKASLRRIMISFGITLL
                     ALLASYTPMALSQFSKGKDTVVIAGKLGAEPDILINLYKELIEDQSDISVELKSNFGK
                     TSFLYEALKSGDIDMYPEFTGTITSSLLRDKPPLSNDPKQVYEDAKKGIAKQDKLTLL
                     KPFAYQNTYAVAMPEKLAKEYQIETISDLKAHADTLKAGFTLEFKDRADGYKGMQSQY
                     GLQLSVATMEPALRYQAIQSGDIQVTDAYSTDAEITKYHLKVLKDDKQLFPPYQGAPL
                     MKTSLLTKHPELKGILNQLAGKITEKEMQDMNYEVSVKGADANKVARDYLLKTGLIQK
                     "
BASE COUNT        11674 a         6350 c         7842 g        12104 t
ORIGIN      
        1 aatagtttaa tttatatcat ttttgcctat gtaacctatc tgatcaatca tattattagc
       61 tatatattta gtacaggtgt ggcattaggt atttcaatgg taggtgttat tgttgcagct
      121 ttttgtgaaa cgagccttgg tgataaggta tggtttctta ttccatgggc atggctttta
      181 agaatatcag atactctata taaccaacag aaaatggcaa ttgttccact tattgttata
      241 ttttttgttt catgcacagt agctttactt catatacgaa tatttaaaag ttggaatcaa
      301 gactgtttga caagtagtta aatacatcta ttttgtttac ctaacaataa atatgatttt
      361 taagttataa atactatcta acttgtttaa ctaaaaaatg actataaaat ttcaatggtt
      421 tactagctta tatttaatag acttgtccaa ataggacaat taaacatttt ttaatcaaat
      481 ctcctttata ctgatattcg gagaatgtta gatgtatccg cgtattagga atttgagaga
      541 agataacgat ttcactcaaa agtttgttgc aaatttactt tccttctctc atgcaaatta
      601 tgcaaaaatt gagagaggag aggttgcctt aatggcagat gttcttgtgc agttttctaa
      661 actctataac gtcagtattg attatttatt agggcaaaca gattatccgt atcgttttca
      721 taataaataa aagtctcgcc ttgatttgtt cattgacaac tgaataaacc gaggcgggac
      781 tttcttgttt gtcgagcaat ttagtgagat acgccgtgat aggagcgata attattagct
      841 ataaaataga gtggttctct atctgccatg accacaaata gggacaatga tacttctgaa
      901 cgttcaggct gccagcgtaa aaggccagtg ggtgagattc ccatgagtga tttaaccagt
      961 catacccacg gaggttgtaa tttttttgaa aggagtggag gtgtgaaaat agtaacatat
     1021 aaaaagcttg atgaatattg gttttctcga acatactgca agaaatatta ttagagaagc
     1081 aaaaagaata gctataaaaa agttgaagaa gctcgcgaac ttgaacaaaa tgcggtacaa
     1141 ttaagtaaat cgccctttga caatagaaga cttggtattg ctcctaaaga tattgtagaa
     1201 gagttaatag gtattctact ctctgaagaa agtttagaaa gtgagaatta aagatgacta
     1261 taaaaaatca aaaaaagtac aaaggtgttt attgtgataa gaatgggaag atattttatc
     1321 aagctgatct tggtgtagat ccagtaacag gtaaacgagt tcaaaagaaa gccagaaaga
     1381 attagtatgg aaagccattt ggtacaatga aagaagcgta tgatgaactg gttcggatta
     1441 ggtatgagtt cagtaataat cttagtattg aaaatgacaa tatatcgttt gaaaactata
     1501 tgaatactat ttacctaaga gcatataagc aaaaggttca ggctgtaact tatgaaatag
     1561 ccttaccaca tcataagtta tttattcagt attttggttc taaacattta aagggaataa
     1621 cagcttgaga ttgtgaatct tttagtttac atattattga aaattatgca aagaatttat
     1681 ggtcgcgatt caaagcttgc atggggtatg ctgaaaggtt aggctatatt tcaaaagtgc
     1741 catgtaaggc tttggataat cctaaaggga aacatcccga cactccattt tggacttata
     1801 ttgagtttca gaattttata aaatcgtttg atttgcaaga ttatgaagag ctacaaagat
     1861 ttacaactat ttggctatat tatatgacag gtgttcgtgt aagtgaaggt ttatcattat
     1921 gatgggaaaa tatcgatttc gaacaaaaat tcttgaaagt tcatacaacc ttggaaaagg
     1981 atgaaaatgg aaattggtat cgtaaagacc aaacaaaaac gccagcagga gaacgtctta
     2041 ttgagttaga tgatgtgaca atagtagtat tggaaaattg gagaagaaat caagtagtca
     2101 atactgacac agatttcatt atttcacggt ttggtgaacc attttgtaaa tcaacgattt
     2161 gtcgagttat taagcacaaa gctcaagaag taggagtacc agtgataacg ggaaaaggct
     2221 tgcgacatag tcatgcttcc tatcttatta atgttttgaa aaaagacatt ctgtacgttg
     2281 caaagtgtat gggacatgct gataagtcta caactttgaa tacatatagt cactgattta
     2341 acgcattaga taaaacagta tctgaagaaa taacccaaaa tgtaataagt tcaggattag
     2401 attccatttt ataccaaaat tcctgccaaa caactaattg atataacgaa gcttattata
     2461 tcgcccatat aacaggatgt gttattgtta cagggtactt gctttatgag cttgtttaag
     2521 ctactaaagg tgtgtgccac tatacttcct tccaaatttc tgagaatttt tgctataata
     2581 gtaactatga aagatataat gataaaaagg tgggttatcc ttttttttat ggtattaact
     2641 tgttcagggt ttggtaaaac agttctcgca gctgattttc aagtgggagc agaacatgcg
     2701 attgtcgtag aggcagactc tgggagagtt ctgtatgaga aagacgctaa gacgccagat
     2761 gctattgcct ctttgaccaa attggtgacg gcttatctgg ttttagataa ggttaaatca
     2821 ggccagctgc aattatcaga tcaagttaat ctctcagatt atgccttcga gttaacaaaa
     2881 gataggtctt taagtaatgt accttttgac aaaaaaactt attcagttca agacctgttg
     2941 acagctactt tggtagcaag ctcaaatagc gctgctattg cgttggccga aaaagtggcg
     3001 ggctcagagc ctcattttgt aaatcaaatg agagaacagc tatctcattg ggggattact
     3061 tctggtaaaa tcctcaacgc ctcaggtctg ccaaatgagg tgttaaagga ccatcgttat
     3121 cctggttcta cgttggaaga agaaaatacc ttgagtgctc aagatgttgc agttgtgacg
     3181 atgcatttgt tagaagattt tcctgagatt ttagagatca ccaaacaaac agaagttaat
     3241 tttgctggca attctattaa aagttttaat caactcttac caggtatggc taagggtaga
     3301 gcaggagtgg atggtttaaa gactgggaca acagacttgg caggtcattg tcttgtggta
     3361 acttctattg aaaatggtat gcgagtgatt accgttattc taaatgccga tggatctgat
     3421 aaaaaccaaa acacgcggtt tgagcaagca aatcgtttgt tggactatgt ggctagaact
     3481 tattgttgtc gtaagattct taaaaaagga agtcttgtct cggagcggtc gctccctatt
     3541 caagatggcc aagtaaaaga actaccggta tctgttgccg aagatgtaac catcattttg
     3601 caacagggtg aacaagttcc caaacctaaa caatttatga tatcagagac ttctttacta
     3661 gcaccgatta ctaaaggtga agtggttgct tatttgacgt ctccaagaat aacagatcag
     3721 tcagtgaggt atttaaagga acctaagcgt attcctctta aagctagcca atctcttaaa
     3781 aaagcctctg acttacagct gtggtggcga gattttcttg aaaaaagacg ttaaagataa
     3841 gaataaaata attttaaaac aatcaaaaaa agacagttat gaaagaattt tttcataact
     3901 gtctttttag tttacagtga gtatgatttg gaatgataaa aaaattgatg ttatggttcc
     3961 attgtttgta aaagattttc cggactcatg tttgggagga tacgaatccg gttaagagaa
     4021 agaagtccat tagccgcgct agctattcct tcgttagtgg taacacctaa tttatagtta
     4081 agtctagctg caatttgtaa agtagtgtca ttatatctac cagatggata agcaatggca
     4141 atagtgtttt ggtttaattg cttatcaaga tagtcctttg aatctttcat ttcagttgtt
     4201 tgaacatcag gactagtctg ctctaaatca ggatgattca ctgtatgatc ttggaaagac
     4261 ataccgaatt gtttcatttc tttcatctgc ttaagagtca gattggcagc gcttcctatt
     4321 tcggttagtc ctgtaataac attattggtg gctttagcat catatttttt taagattggg
     4381 taagcaacgt tgtagaaatc aatcatactg tcatcaaatg ttagccatac aactttttta
     4441 gcaggcaatt cattatttga aagagcgcgg tagacctctt caggactcaa aaaatagtag
     4501 ccctcgtctt tcattttttg aagttgttga tcaaagagat ctgggttgac aatcaaatta
     4561 gcattggccg tctcttcagg ggacattaca tgaatagcat gatacattaa aatggggatc
     4621 ttgacaggtg tttcttgctt ggtccaagtt aatttagaat gggccttttt ttgagatttt
     4681 gaggaatcga aatgagtctt cttaagtttt gtcgtttctt tttttgccgt ctttttgtga
     4741 tgaaaaagac ttattttagg tacaaattga gagacatctg acaagccgaa tttgttagac
     4801 ccttggtata aatagaaaat gagggtcaaa ccaagaatgc aggcaaataa tagtataagg
     4861 ttaatgagag tgaaggcttt tcttttttgg cgccgtttat gtctattgtt cattacttgc
     4921 tccttttttt gatataagta cctctattgt aacatttttt agagaaaaga aggcttgaag
     4981 tcagcctttt ttcttgtttt ttggaagaac gtgtattaag gagtaaaaaa attattgtca
     5041 cagataacac tttaggaggt agcaaagact atgtggggct ggtattctta tcacgtaaaa
     5101 aaagcaatga agataaaagt gtagtgtact ttagaactta tttgagtgtt tggtgaggat
     5161 aataaaaaaa agggtactac tctaggttat ctgctgtcac ttttttttat atatggtata
     5221 ctaaagatag atgtgaggtg tgaaatgact tatgaagaga ctctagaatg gattcatgat
     5281 cacttagttt tcggcatcaa gccaggctta aaacgtatgc tttgggttct tggacagtta
     5341 ggaaatcccc aaaaaaatgt caagggagtg catatcgttg gaactaacgg aaaaggttcc
     5401 acggtcaatc atttacaaca catttttacg acagctggtt atgaagtggg gacctttacc
     5461 tctccttata ttatggattt caaagaacgt attagcataa atggtcgaat gatttcagaa
     5521 aaagatttgg ttattgctgc taatcgtatt cgtcctttga cagagcgtct agttcaagag
     5581 actgattttg gagaggtaac tgaatttgaa gtgattactt tgatcatgtt cctctatttt
     5641 ggagacatgc atcctgtaga tattgctatt attgaagcag gccttggcgg tctttatgat
     5701 tcgaccaatg tttttcaagc aatggttgtg gtatgtccct caattggttt agatcaccaa
     5761 gctattttgg gagagactta cgctgatatt gccgcccaaa aagctggtgt tttagaaggg
     5821 ggcgagaccc ttgtttttgc cgttgaaaac ccgtcagctc gagaggtttt tttaacaaaa
     5881 gctgagcagg ttggtgcttc aatctgggaa tggcaagagc agtttcaaat ggctgagaac
     5941 gcatctggct atcgttttac tagtccttta ggagttattt cagatattca catcgctatg
     6001 ccaggacacc accaagtgtc taatgcagct ttagctatta tgacttgctt aaccttgaaa
     6061 gaccgctatc ctaggctaac ttcagatcat ataagggaag gtctggcaaa tagtttgtgg
     6121 ctaggccgta ccgaattatt agcaccaaat ctaatgattg atggggctca taacaatgaa
     6181 agcgttgctg ctttggtagc cgtcttaaaa aataactaca atgataaaaa gcttcatatt
     6241 ttgtttggtg cgatcgacac caagcctatt gcggatatgt tggttgctct tgagcaaata
     6301 ggtgacttgc aggttactgg ctttcattac cctaacgcct atccattgga aaaataccca
     6361 gaacgttttg gtagggttgc tgatttcaaa gatttcttgg ccctgcgtaa gcatgccaaa
     6421 gcagatgact ttttcgtgat tacagggtcg ctatatttta tttcagaaat tagacagtat
     6481 tggaaaaaac atattgaaaa aaacgttctt ttaacccatt aaaaaagaga aaatgaaaag
     6541 gaaaaggaaa aaactaatga aaagggaaag actaatgtct attaataagg aaaaagcaga
     6601 agcagccatt tatcagtttt tagaggctat tggtgaaaat ccaaatcggg aaggtcttct
     6661 tgacacgcct aaacgggttg ctaaaatgta tgcggagatg tttttgggac tgggaaaaga
     6721 tcctaaagag gagtttacgg ctgtctttaa agaacaacat gaggatgtgg tgattgtcaa
     6781 agacattagt ttttattcta tatgtgaaca ccacttagtt cctttttatg gcaaggctca
     6841 tatcgcttac ttgccaagtg atggtcgagt aacaggtttg agtaaattag cgcgagcagt
     6901 tgaagtagct agtaaacgac ctcaactcca agagcgtttg acctctcaaa tagcggatgc
     6961 tttagttgaa gccctcaatc ctaaagggac tttagttatg gtagaagcag agcacatgtg
     7021 catgaccatg cgaggcatca aaaagccagg tagtaaaacc attaccacca ctgccagagg
     7081 cttatataag gaaagtcgtg ctgagaggca agaagttatt tctctaatga caaaagatta
     7141 ggagaactta tgaaaattgg aaagtttgtg attgagggca atgcggctat catggggatt
     7201 ttaaatgtga ctccagattc tttttcagat ggggggtctt acactactgt gcaaaaagca
     7261 ttagatcacg ttgagcaaat gattgctgat ggggctaaaa tcatcgacgt tggtggagaa
     7321 tcaacacgtc caggttgcca atttgtaagc gctaccgatg aaattgaccg ggtggttcct
     7381 gtgatcaagg ccatcaaaga aaactatgat attctaatca gcattgatac ctataaaacc
     7441 gaaacagcta gagcagcttt agaggcgggt gccgatattc tcaatgatgt ttgggcaggt
     7501 ttgtacgacg gtcaaatgtt tgccttagca gccgagtacg atgcgcctat catcttgatg
     7561 cataaccaag acgaagaagt ttatcaagag gtaacacaag acgtttgtga ttttctaggc
     7621 aatagagcac aagcagctct tgatgctggc gtgccaaaaa acaatatttg ggttgatcca
     7681 ggatttggat ttgccaaatc tgttcaacag aatacggagt tattaaaagg attggaccgc
     7741 gtctgtcatt tgggctatcc tgtcttgttt ggtatttcga gaaagcgtgt cgtagatgcc
     7801 ttgttaggcg gcaacaccaa agctaaagag cgagacggag cgacagcagc cttgtctgct
     7861 tatgcccttg gaaaaggctg tcagattgta cgcgtacacg atgtcaaggc taatcaagac
     7921 attgtggctg tgttgagcca gttgatgtga ggacttatgg ataaaatcgt attagaaggt
     7981 tgccgttttt atggctacca tggagccttt aaagaagaac agacccttgg gcaaattttt
     8041 ctggttgatt tagaattgtc agtcgatttg caagcagctt ctttgtcaga ccaattaaca
     8101 gatacggtcc attacgggat ggtgtttgat agtgtccgtc agttagtgga aggagaaaag
     8161 tttattttga ttgaacgttt agcaggtgcg atttgtgaac agctttttaa cgaatttcca
     8221 cctattgaag ccatcaaagt ggccattaaa aaggaaaacc caccgattgc aggccactat
     8281 aaggcagttg gtattgaatt ggagagacag agatgaccat tgtttattta agtttaggca
     8341 ccaatatggg ggaccgagca gcttacttgc aaaaagctct tgaggcttta gctgacttac
     8401 ctcaaacacg gttgcttgct caatcatcca tttatgaaac gactgcttgg ggcaagacag
     8461 gccaggctga ttttctcaat atggcctgtc aattagatac acaattaacc gcggctgatt
     8521 tcttaaaaga aacacaagct attgagcaat ctcttggtcg tgtgaggcat gaaaaatggg
     8581 gatcaagaac tattgacatt gatatcttgc tatttggaga agaggtttat gacacaaagg
     8641 aattgaaagt gccacaccct tatatgactg agcgtgcttt tgttttaatc cccttattgg
     8701 aattgcagcc agatttaaaa ttgcctccta atcataagtt tttaagggat tatcttgcag
     8761 ctttagatca atcagatatc acgctctttt cagctcaaca gacagagttc tagactctgt
     8821 tttcggagta atgcctgcta taagccaatc cataaagttt tgccaaattc atcgttattt
     8881 tttctaaaat ttgatataat agtatcggct ttatgccgat ttttttacgc gtataagaag
     8941 tgataaaaga aagaagataa ctatgataac tgaacttcat gggattgata ttcgagagaa
     9001 tgaaccccta aaacattaca cttacactaa ggtaggtggc ccagcagatt tcttggcttt
     9061 tcctagaaat cactacgagc tatcacgcat cgttgcttat gccaacaaag aaaatatgcc
     9121 ttggcttgtt ttgggaaatg ccagcaatct cattgtgcga gatggtggta ttcgtggctt
     9181 tgtcatcatg tttgataagc taaatgcagt gcacttaaat ggttatacct taaaagccga
     9241 agctggtgcc aatctgattg aaacaacgaa gattgccaaa ttccatagtt taacagggtt
     9301 tgaatttgca tgtggcattc ctggaagcat tgggggtgct gtttttatga atgcgggtgc
     9361 ttatggtggg gaaatatcac atattttctt atccgctaag gtgcttaccc caagtggaga
     9421 gatcaaaacc atttcagcta gggatatggc ttttggttac cgtcactctg ccattcaaga
     9481 aacaggtgac attgtcattt ctgctaagtt tgctcttaag ccgggtaatt atgatacgat
     9541 cagccaagaa atgaataggt taaatcacct tcgccaacta aaacaacctt tagaatttcc
     9601 atcttgtgga tctgtgttta agcgcccgcc aggacacttt gcaggtcaat taatcatgga
     9661 agcaaatctt aaagggcatc ggattggtgg tgtggaagtt tccgaaaaac acgctggttt
     9721 tatgattaat gtggcagatg gcacagctaa agattatgaa gatttgattg cttatgtcat
     9781 tgagacagtt gaaaaccatt ctggtgtcaa gcttgaacca gaagttcgga ttattgggga
     9841 aaacctgtaa ccattattga taattaaaga aagtaccgtg gaggatttat gacaattgac
     9901 taagccaatt atcacattta acaatgtttc aaaaacattt gaggacagtg gaacacaggt
     9961 tctaaagaac attaactttg accttgaaga aggaaaattt tacaccctgc ttggagcttc
    10021 tggctctgga aaatcaacta ttttaaatat tatggcgggc ctactagatg ccagcagcgg
    10081 agacatttat ttagatgggg aacgtattaa tgatttgccg attaataaac gtgacattca
    10141 taccgttttc caaaattatg ccctcttccc tcatatgact gtttttgaga atgttgcctt
    10201 tgctttgaag ctgaagaaag tggacaagaa agagattgct aagcgcgtga aagaaacctt
    10261 gaaaatggtt caattagaag ggtatgaaaa tcgttctatc cagaagttat ctggtggtca
    10321 acgtcaacgt gttgccattg cgcgtgcgat tatcaatcaa ccgcgtgtgg tcttacttga
    10381 cgaaccactc tcagcccttg atttgaaatt aagaacagag atgcaatatg aattacgtga
    10441 attgcaacaa cgtctaggca ttacctttgt ttttgttact cacgatcaag aagaagcctt
    10501 ggccatgagt gattggattt ttgtcatgaa tgaaggtgaa attgttcagt cgggaacacc
    10561 agtggatatt tacgatgagc caattaatca ttttgttgct aattttattg gagaatctaa
    10621 tattattaac ggtaccatga ttgaagacta tcttgtctcc tttaacggga aagaatttga
    10681 atctgtggac ggtggaatgc gccctaatga gcctgttgaa gtggttattc gtcctgaaga
    10741 tcttcaaatt actttgccag aagaagggaa attacaagtt aaggttgata cccaattatt
    10801 ccgcggggtt cactacgaaa ttattgccta tgatgaattg ggtaatgaat ggatgattca
    10861 ttctacccgc aaagctatcg agggagaagt tatcggatta gactttaccc ctgaagatct
    10921 tcatatcatg cgtcttaatg agactgaaga ggaatttgat gcccgtattg aagaatatgt
    10981 ggaaatggat gagcctgaag atggattgat taatgccatt gaggaggagc gtaatgaaga
    11041 aaacctctag tcttttttcg attccttact tcttatggat tctctttttt gttgtggcac
    11101 cagtcactct cttgttttac aagtcctttt ttgacataga agggcgcgtg accttagcca
    11161 attatgaaac cttttttagc tcttggacct atttgagaat gagtgtgaat tctattttat
    11221 acgctggtat tatcacactc gtcacgctct taatttcata tcctacggct ctctttttaa
    11281 cgcgcctaaa gcacaagcag ttgtggctta tgctcattat tttgccaact tgggtaaact
    11341 tattgctaaa agcctatgcc tttatgggaa tctttggtca acaaggaggg attaacagct
    11401 ttttaacctt tatggggatt ggcccgcagc aaatcctttt cacggatttc tccttcattt
    11461 ttgtagcctc ttacattgag cttcctttta tgatgttacc gatttttaat gctttggatg
    11521 atattgacca taatgtcatc aatgccagtc gcgatctagg agctagtgaa tttcaggcct
    11581 tctcaaaagt tatttttccc ctttctttaa atggggttag ggcaggtgtt cagtctgtct
    11641 ttatcccaag tttgagtctc tttatgttaa cccgtttgat tggtggaaac cgcgtgatta
    11701 cacttggtac agccattgaa caacattttt tgaccaccca aaactgggga atgggatcaa
    11761 ccattggtgt cgtcttgatt ttgaccatgg ttgctattat gtggctcaca aaggagaaaa
    11821 gtaaatgaaa aaatttgcca atctttattt agcgagtgtc tttgttttac tctacattcc
    11881 tattttttat ttgattttct attctttcaa caaaggtggg gatatgaatg gttttacagg
    11941 atttaccctt gagcattacc aaaccatgtt tgaggatagt cgtctcatga caatcttact
    12001 gcaaaccttt gttcttgctt ttagtagcgc tctactagca acgattattg ggacctttgg
    12061 agctatcttt atccaccatg ttagaggtaa gtaccaaaat gccatgctat cagccaataa
    12121 tgtcttgatg gtatcaccag atgtcatgat tggggcttcc tttttaattc tttttacatc
    12181 attgaagttt cagctgggca tgtcttcagt tttattaagt catattgctt tttcgattcc
    12241 tattgtggtt ttgatggtat tgccgcgctt gaaagagatg aatcaagaca tggtcaacgc
    12301 cgcttatgat ttgggagcta attatttcca aatgctcaaa gaagtcatgc tgccatactt
    12361 aacaccaggg attattgcag gttattttat ggcctttacc tattccttag atgattttgc
    12421 agtgactttc tttttgactg gaaatggttt tactacttta tctgttgaga tttattcgcg
    12481 ggctcgtcag ggaatttcct tggatatcaa tgctttgtca accatcgttt tctttttctc
    12541 catcctctta gtgatcggtt attattatat gtcacaggac aaggaggaaa aacatgcgta
    12601 aactttattc ctttctagca ggagttttgg gtgttattgt tattttaaca agtctttctt
    12661 tcatcttgca gaaaaaatcg ggttctggta gtcaatcgga taaattagtt atttataact
    12721 ggggagatta cattgatcca gctttgctca aaaaattcac caaagaaacg ggcattgaag
    12781 tgcagtatga aactttcgat tccaatgaag ccatgtacac taaaatcaag cagggcggaa
    12841 ccacttacga cattgctgtt cctagtgatt acaccattga taaaatgatc aaagaaaacc
    12901 tactcaataa gcttgataag tcaaaattag ttggcatgga taatatcggg aaagaatttt
    12961 tagggaaaag ctttgaccca caaaacgact attctttgcc ttatttctgg ggaaccgttg
    13021 ggattgttta taatgatcaa ttagttgata aggcgcctat gcactgggaa gatctgtggc
    13081 gtccagaata taaaaatagt attatgctga ttgatggagc gcgtgaaatg ctaggggttg
    13141 gtttaacaac ttttggttat agtgtgaatt ctaaaaatct ggagcagttg caggcagccg
    13201 agagaaaact gcagcagttg acgccgaatg ttaaagccat tgtagcagat gagatgaaag
    13261 gctacatgat tcaaggtgac gctgctattg gaattacctt ttctggtgaa gccagtgaga
    13321 tgttagatag taacgaacac cttcactaca tcgtgccttc agaagggtct aacctttggt
    13381 ttgataattt ggtactacca aaaaccatga aacacgaaaa agaagcttat gcttttttga
    13441 actttatcaa tcgtcctgaa aatgctgcgc aaaatgctgc atatattggt tatgcgacac
    13501 caaataaaaa agccaaggcc ttacttccag atgagataaa aaatgatcct gctttttatc
    13561 caacagatga cattatcaaa aaattggaag tttatgacaa tttagggtca agatggttgg
    13621 ggatttataa tgatttatac ctccaattta aaatgtatcg caaataagca ctcaaaaagg
    13681 ctggtatttc tccagccttt ttaatgtatc gacagatgac gtcaccagta tacgcgatag
    13741 ggacggccaa ccttggtaaa aatttgttgg ctgttcagtt gcttattttc ttctaaataa
    13801 gcaatgtatt tacggacaga gacatgggat aagtgacaag cggaagctaa ttcttggata
    13861 gtaaaaggtt gatcaaaggc tttgatattt tccataatcc actggaaagt ggattcagat
    13921 aagccttttt ctaacaattg tttgttttta gtgtcttttt tgctagtcaa acatttcagt
    13981 tggtcaattt gggcctgctc tagttgctga ttggccaaat gttctcgatg tgtaacaaac
    14041 tgttggatgc tttcttgaaa gcgttcaaag gtaaagggct tgatgaggta gtcgataatt
    14101 cctaaatgaa aaccatctcg gataatattc ccatcatttg cggctgaaat aataatgact
    14161 tcgcaaggaa tatgctgagc acgccatttc tccaaaaatt gaatcccatt gccatctgta
    14221 atatgaatat ccagtaaaat gaggtcaatt gcgtaatcag taagtatgct ttgaacagct
    14281 ttcatagagt cgctggatat aatgcgatca aaaagattta gtttttcgag gtaattgcga
    14341 tggataaaat ccaccatagg atcatcttca atgattaaaa cgttcatgtt gccctcctac
    14401 ataaggtgtt gtcactgaga actgtaactc ggttacggtt tgatgttgat ggaattggga
    14461 atttgtatcg gtcaagagct gttgaaaata aggtaagtca aaaataggct gtacccgctc
    14521 caaatcaatc caattgtctg tcaaggtgta atggctgatt aaatgattct gatcatgttg
    14581 aatactcaaa acgagtgatt tgggtctcag tgtggttaga agattagtat ggataaagcg
    14641 gtaaatcatc aaaccattat taagttgatt cttggtggca gaatgaggaa tctcgcttaa
    14701 aacatcaatc ttgagatgga catttaattc ttggtatttc tcttgctctc caatgagaaa
    14761 actagctaga agaggctcct taacaaggac agataagcta gtgagaattt cattttcggg
    14821 ctccaaaata ctgtctaaat aaattttcaa ttggtcataa taagcaatat ccaccaagcc
    14881 ataaataaca tgcaattgat tcatgaattt atgagtttgg gcttgtagag cagaagcata
    14941 ggcggtggta taggctagct gatccaaggt atcaatagcc ttaactgctt ctcgcatgaa
    15001 aataatgtgg cctcggtgat cgtttttgac acagatgggt gaaatggcta acaagtagtc
    15061 ttcctcacca taccgaaaga gttgttcatg cccctcttgc aaactaagtt ttggaaaatc
    15121 aggaaagagg tgattgaaac tgttgccagt aggttttccc aactggcatt tccgagcaat
    15181 taatttttgt ccagcctgat tgcagagctg taagatacca gctttatcta ccacaaatac
    15241 ggcggcttcg atttgatcca acatggcatt acgctcttca aagagctgat aaatttcact
    15301 tggttctaac tggtgcagtt gacgcttgag tcgaaaagaa ataaaactgg tgaccagtaa
    15361 actaataaga agacaaagga gtaaagacag ggtataattg cgttttgacg ttaaagccac
    15421 atcatttagg gtagttagtt taatgccaac cgcaatagca ccaatctgtt tgtcaccgtc
    15481 aaacacaggt actagatagc gaagtgactt tcctaaagtg cccttggcgg tagagataac
    15541 cttttttcct gctagaacag cctcctcatc acctccttga aaaggttttc cgatattttt
    15601 cgggttagga tgggtgagtc gaatcccttt catgttcatc acgaccacat aatcaaggtt
    15661 atagatggag gcaatggagt tggtataggc tgttgtttta gcatttggtt ggttgtttag
    15721 gagtaattct ttaatcgctt gatgacttgc taacattttt ccagttgacg ttagcaaatg
    15781 ggtttcctga tttttgatgg attggtgcgt atcatgtagc ataatcccgt aaaatagaga
    15841 ggttgtgaca acaatcatag ataccaaaat caacgataaa ctggcccata aacgtagtgg
    15901 ttttttcata aggtctcctc tttattgagt ataacacgat aagaaatgac ctgctagctt
    15961 aaattaatta aataacttga aaaaattaaa gtagttaaac aacttaaata aactatgctt
    16021 ttgacatgtt ctcttttata ctagccttat cactaaagga ggttgtttca tggaaacaat
    16081 aagcaagaaa atgcctcaaa aagacttgtc tgagcattca aaagcatggc aaaataggag
    16141 aataggctcc gttcccttgc ctgtttatct ggttttagct accttaattt tagtcacagg
    16201 ctggctccaa caattgcctg ttaatatgct gggtggcttt gctgttattt taacccttgg
    16261 ttggctacta ggaaccattg gagcgaccat tccaggcctc aaacattttg gtggcccagc
    16321 tattttatca ctcttagtcc cttcgatttt ggtttttttt aatctcctca atcccaatgt
    16381 cttagaagcg acgaatgtct tgatgaagca agccaatttt ctttattttt atattgcctg
    16441 cctggtctgc ggtagtattt taggcatgaa tcgaaaaatc ttgattcaag gtctttttcg
    16501 gatgattatt cccatgttac ttggaatggt ttgtgcaatg ggtgtaggga ctttggttgg
    16561 cgtcatttta ggattagact ggcaacatac cctcttttat gttgtgacgc cagtccttgc
    16621 tggaggaatt ggtgaaggta ttttaccctt atctcttggt tacagtgcca ttacaggagt
    16681 cggaagtgag caattagtgg ctcaactgat tccagccacc attattggga atttctttgc
    16741 tatcttgtgc acggctttgc tcaatcgttt tggggagaag catccaagtt attctggtca
    16801 agggcaactg gttaaaattg gtcacagcga aaatatgtct gatgccctaa aagacaattc
    16861 tggagcctta gatgttaaat taatgggggc aggagtttta acagcttgtt cactttttat
    16921 tgccggtggc ttgttacagc atttaacagg ttttccagga ccggtgttga tgattatctt
    16981 ggcggccttc ttgaaatacc tgaatgttat cccccaagaa acacaaaatg gtgccaaaca
    17041 actttataaa tttatttcca gcaactttac ttttccacta atggcagggc ttggactcct
    17101 ttatatccca ttaaaagatg ttgtggccac ccttagctgg caatatttta ttgtggtgat
    17161 tagtgtggtg ttaacggttg tcagtgtcgg tttttttgtc tctcgtttcc ttaacatgaa
    17221 cccagtggaa gcagctatta tttcagcttg tcaaagtggt atgggtggaa caggtgatgt
    17281 ggctattttg agtaccgcag accgcatgaa tctaatgccg tttgcccaag tcgctacccg
    17341 tttaggtggg gctattaccg ttatcactat gacagctatt ttaagaatta tttttagtta
    17401 atccaaacca ataaaaaaga gaggtattcc ctatggaaaa tcaattaggt caacttgccc
    17461 ttgaacaagc taaaacgttc ggagggaaat tggaagttca accaaaagtt gacattaaaa
    17521 ataaacatga tttgagcatt gcctatacac caggtgtagc tagtgtgtca tctgccatcg
    17581 caaaagataa aacattagca tatgacttaa ccaccaagaa aaatacagta gccgttatca
    17641 gtgatggtac tgcggttctt ggtctggggg atattggccc tgaagcagcc atgccagtta
    17701 tggaaggaaa agcagccctt tttaaagcat ttgcgggtgt cgatgctatt ccgattgtat
    17761 tagataccaa agatactgaa gaaattatta gtattgtcaa ggcgcttgca ccaacctttg
    17821 gtggtattaa tttggaggat attagtgcgc cccgctgttt tgaaattgag cagcgcttga
    17881 ttaaggaatg tcatattcct gttttccatg acgatcagca tggaacagct attgtggtgt
    17941 tagcagccat cttcaatagt ttgaaacttc ttaaaaaatc ccttgatgag gtatccattg
    18001 tggtgaacgg tggtggatct gctggattat ccattacccg taaattatta gcagcaggtg
    18061 ctacgaaagt gacagtagtg gataaatttg gcattatcaa tgaacaagaa gccgctcaat
    18121 tagcgccaca tcatttggat atagcgaaag tcactaatcg tgagtttaaa tcaggtacct
    18181 tagaagatgc tcttgaagga gcagatatct tcattggcgt atcagcacca ggagtcttaa
    18241 aagcagagtg gattagtaaa atggcagcgc gcccagtaat ttttgccatg gccaacccta
    18301 ttccagagat ttaccctgat gaagcccttg aagcgggggc ttacattgta ggtactggcc
    18361 gcagtgattt tcctaaccag attaataatg tccttgcctt tccaggtatt tttaggggag
    18421 cattagatgc acgggcaaaa accattactg ttgagatgca aattgctgcc gcaaaaggta
    18481 ttgccagtct tgtcccagat gacgctttga gcactactaa tattattcca gatgccttta
    18541 aagaaggagt cgctgagatt gtcgctaaaa gcgtcaggtt ggtagtcctt aaatcataga
    18601 atatatgaaa aaaagagaaa gttatacttt aaatcacaaa ataagtagaa gcatatttct
    18661 agcttctact tattttgtga ttagctaata tcattatcaa taataacttt caaggcgtta
    18721 tttgcaccag catgtttaaa ggtttcataa gctttttcaa cttctgaaag tttaaaatga
    18781 tgtgtaataa gtcttgttgc gtcaatcttt ccagttttca agacatttag tagcatttca
    18841 gttgtattgg cattaacaag tccagtgttt aaggtgatat ttttaatcca taattcatcc
    18901 aggttgaaat caactggttt cccatgaacc ccaacgttag caatatgccc acctactgat
    18961 ataatttttt gacaaatatc aaatgtcgcg ggataaccaa cacactccat ggaaatatcg
    19021 acaccacgtc cattggttat atcgtcaatg atagccttga cttcttcact acttcctgaa
    19081 caaatcgtgt gtgtggctcc aaatgtttta gctgcttcaa ggcgattttg agataagtcg
    19141 accataataa tattggctgg agagaaaaat tgaacggtta gtagggctgc gagtcctact
    19201 ggaccggcac caacaataca aacattatct ccaggcttga catgtgatgg caagacacca
    19261 atttcatatg aagttggtaa aatatcagaa agcatgacaa gtgcctcatc atcaatcgta
    19321 tcaggagcat gatagagact accatctgca tgtgggatat ggacatattc tgcttgtgtt
    19381 ccattaatta aatggccaag aatccaaccg ccatcttcac aatgtgacga aaggcctcgt
    19441 ttacaataat aacaggtatg acatgatgtg acgcaagaaa taatcacttt atctccgatt
    19501 ttaaagttag taaccgcatc accgacctcc ttaacaattc caattccttc atgaccaagg
    19561 attgttcctt ctttacatgc gggaacatcc ccacctaaga tatgcaaatc agttccacaa
    19621 atagtggttt ttaccaactg gacgatagca tctgtaggct ttataatgac tggttttggt
    19681 ttgtctatga gctgtagatt tccggttgaa agataagttg ctgctttcat atttaactcc
    19741 ttataaggtt gagaggtaat caaccaattc atttttacac agatattata atagtttaag
    19801 aaagggtttt caatattttt taaatcgatt atgataattt tttagatcat ctttgttgaa
    19861 taacttcatc cttattcaga acttaaaggg tactattata agaatttgaa accgctttac
    19921 ttaaatcgtg tatttttgta caataatgat taaggaggtt tttagatgaa aaaagaattt
    19981 accagtatcc tgtttacagt tagtttttgt ggaatcatag cattgcctgt cgaagctagt
    20041 ggccctaaag ttccttatac acaagaaggt ataacggcca tttcaaatca ggcaacagta
    20101 aaattgattt ctattgctga tatcgcgagc agtttagaag gacaaaaacc tataacggtt
    20161 agttttgata tcgatgatac tttgttattt actagccaat attttcagta cggcaaagaa
    20221 tatataactc caggctcatt tgattttttg cataagcaaa aattttggga cttggtcgct
    20281 aaacggggag atcaggattc tattccaaaa gagtatgcaa agcaactaat cgcaatgcat
    20341 caaaaacgag gagataaaat tgtctttatt actggccgaa ctcgaggatc gatgtataaa
    20401 aaaggtgaga ttgataaaac agctaagtca ctggctaaag attttaagtt agacaagcca
    20461 atagctatca attatacagg agataaagct gtgaagccgt atcagtatga taaaacatat
    20521 tatataaaga aaaacggctc acaaattcat tacggagata gtgatgaaga tataaatgct
    20581 gccaaagagg caggtgcaag acctattcgc attttaagag ctcctaattc aaccaatctt
    20641 cctttgccaa aagctggagg atacggagaa gaggttttag aaaattcggc ttattagtca
    20701 aatatttgtg taagaaagga agtaacggta atttctttca tcaccccact gagtaaccaa
    20761 tagcccagtg ggttttttgg gttacagaaa tcagttctaa aaaagaatca ctggtaactt
    20821 aaggttgtct ttgatacaat ggtaacagtg tatttaaaag aaagagagga caacatggaa
    20881 aaccataaaa atgaattcac cttttccaac aaatcaatta ttgcctatgt ttggcgtggt
    20941 gttatagttg gtattattgc tggtgttatt gttagtttat tccggttgtt aattgaagtg
    21001 acagctgact gggtgattga atggtaccga tacgctcata tcaattcgct gttgctactt
    21061 cctatactaa gtgtcagttt attagctgtc ctttttgtag gttttttagt aaagtctgat
    21121 tctgatataa aaggatcagg gatcccccat gtcgaaggag aactaaaggg actaatgtcc
    21181 ccagattggt ggagtgtcct ttggaaaaaa tttttaggag gaatcatggc tatttctatg
    21241 ggatttatgc tcggtcgtga gggtccttcg attcagttag gtgctatgtc agccaaagga
    21301 ttagctaagt ttttaaaatc gagtcgtttg gaaaagcgag tgctaattgc cagcggagca
    21361 gcagcagggc tatcagcagc ttttaatgct ccaatagcag gcctcttgtt tgttgttgaa
    21421 gaaatttacc atcatttttc gcgtttaatt tggattacag cgctagtggc tagcttagtg
    21481 gctaatttta tttctctgaa tatttttgga ttgaaaccag tcttagcaat gtcagaggcg
    21541 atgccttttt taggactcaa tcaatattgg ttgttgcttt tacttggatt atttctaggc
    21601 tgcttaggct atttatatga gatagttatc cttaatttta ataaacttta tgttatttta
    21661 gggagttggt tacatctgcc agcttatttt tacggtatta ttatggtttt tctaatttta
    21721 ccgattggct attatttacc acagttactt ggaggaggac atggtttaat actttcttta
    21781 tccaatcaac aattgccttt gatgactatc ttcttttatt ttattattcg atttattgtt
    21841 agtatgttta gttatggaag tggcttacct ggaggtattt ttttacctat tttaacttta
    21901 ggtgctttgg caggcctatt atttgggcaa atagcttctc aattaggttt attgaaccaa
    21961 tcttttcttt ctcttttttt gatattaggg atggcagggt attttgcggc aatttcaaaa
    22021 gcaccactga caggcatgat tttggtaact gaaatggtag gtgatttgaa acccttaatg
    22081 gccatagctg tagttacttt tgtctcctat ttagtaatgg atttgttaaa tggacagcca
    22141 atttatgaag ctatgctaga taagatggca atgaagcatc caactaatct tgttgaacca
    22201 acattaattg aattgactgt tggtgataag attgcaggga aatatgttaa agagttaaag
    22261 cttccagaaa atgtcttaat tacaacccaa attcaccatc aaaaatctca agttgtgtca
    22321 ggtaacacgc gccttttatc aggagcaact atctttttgg tcgttaatga agctgataca
    22381 ggttttgtcc gtgaggtatt gatgtaggta ttaatttatc acaattcgga aagttcgagc
    22441 ttgtctttta gactaagtct tcgaacgttt tactgctaga gtttcatctg aaccaaagtg
    22501 aaacgctttt gatgaagtca agtaggcttt ttgttatact aacaataaag ataagctaag
    22561 gaggctgaga tgttagaaat tgtttcagaa gagctacgtc actatcaaga acaaaaattg
    22621 attgagtacc gtaacaaaaa tcagttagcc ccaaaaggag gcattgtatt tgcaggtgat
    22681 tctctcattg aattttttcc tttaaaaaaa gcttttggtt catgtttgcc tattattaat
    22741 cgaggtattg ctgggataga tagtcagtgg ttattgcgcc atttttctgt ccaaattact
    22801 gatttagagc ctaaacatat ttttttatta attggttgta atgatatcgg gcttggatat
    22861 gataaatgtc atattgtcaa gactattgta gagcttatta gccagattcg tagtcattgt
    22921 gtctacagtc agatttatct cttatcttta ttaccagttt ccaacaatcc ccgatatcag
    22981 aagactgtca aaataagaac aaatgctatg attgatgcta ttaataaaga tttagcaatg
    23041 attccaacta tagaatttat taatcttaat acttgtctta aagacgaaaa aggtggttta
    23101 tcagatgaga atactctaga tggtttacat cttaattttc cagcttatgc taaactagct
    23161 gaaattattc aagcttatat ctagcaaata aatgtactaa tagcgatgag gcaagaagtc
    23221 agatagcaga tggttcatca atcaattaag aaaaaagtta tcaagatacc attaaaaata
    23281 gttgctcaaa gttctttcaa atcaaaagat tatgatatcg ctatgttgag ttttagtgta
    23341 taatgataat taaaggagtt accatgaaat ttgaaaaaaa acaagtcttc tatttagtcc
    23401 ttacgtttat cttatgctat gggatcctag caaactggcg aaatggaacg gctatcgtca
    23461 cgactattta caaaacaagc cttccttttt tttatggagc agcaggtgcc tatattgtta
    23521 atattgtaat gagcgcttac gaaaaggtat atgtttatat ctttaaagac tggtcacatg
    23581 tcttaaaagt aaaacggggt atttgtttac tgttagcgta tttgacattt tttattttaa
    23641 ttacttggat tatttccatt gtcattccag acttaattac aagtattagt actctaacaa
    23701 aatttgatac tataacgata caagaagttg ttaacaatct tgagcacaat aaactgttag
    23761 cacgtactat tcagtatatt ggtggcgatg gcaaacttac agagacgatt gctaactata
    23821 gtcaacagtt attaaagcaa tttcttactg ttttgacaaa tattttaaca tctgtcacgg
    23881 ttattgcttc tgcaattatt aatcttttta ttagttttgt tttttcgtta tacgttttag
    23941 caagtaaaga agatctttgt cgtcagggaa atactttagt agatacttat actggtaagt
    24001 acgctaaacg cattcattac ttgttagagt tgttgcatca gcgttttcac ggattttttg
    24061 ttagtcagac cttagaagct atgattttag gttcgctgac tgctagtggc atgtttatat
    24121 taagattacc atttgcaggg actattggcg ttttagtagc ttttacagca ttgattccag
    24181 ttataggtgc ttcaattgga gcagctattg gatttatttt aattatgact cagtccatgt
    24241 cacaggccat tatttttatc atttttttaa ttattttgca gcagattgaa ggcaatttta
    24301 tttatccgaa agtagttggt ggatcgattg gattaccggc tatgtgggta ttaatggcaa
    24361 ttgcaatagg cgcttcttta aagggaatag ttggtatgat tattgcagtt cctttagcag
    24421 cgacacttta tcaagtgatt aaagataata ttcaaaaaag acaagctatt caaaaaaaac
    24481 aagtttccta aaaggaactt gtttttttgt cgaataaaaa gatggaggta atatgataaa
    24541 tgtattctat aaaatgtgat gataataaag ccatgccaag agaacgtttg atgcgactag
    24601 gagcagagtc tctaagtaat caagaattat tagcaatttt attacgaaca ggtaataaag
    24661 aaaagcatgt cttagagctg tcatcctatc ttttatcgca tttagacagt ctggcagatt
    24721 ttaaaaagat gtctttgcaa gaattacaac atttggcagg tataggaaaa gttaaagcga
    24781 ttgaaattaa agctatgatt gagttggttt cccgaatttt agcgacagat aagacattaa
    24841 ctgatagcgt attaaccagt gttcaggtcg ctgaaaaaat gatggcagct ttaggagata
    24901 aaaaacaaga gcatttagtc gtattgtatt tagataatca aaatcgtatt attgaagaaa
    24961 aaactatttt tattgggact gtccgacgtt cacttgcaga accaagagaa attttatact
    25021 atgcctgtaa aaatatggcg actagtctca ttgttattca taatcatcct tcaggaaata
    25081 ttgaacctag ttctaacgat tattgcttta ctgaaaaaat aaaacgatca tgtgaagatt
    25141 taggcattat ctgtctagat cacattatcg ttagctataa agattattat agttttcgag
    25201 aaaaatcaac ccttttttaa atcagaagtc attgacgaat aagcggaaca attgtagatc
    25261 ttcatctcga acaccttgga gaagttcagg atgccactga acacctaaaa aaggaatggc
    25321 atcgttggtt gatatgactg cttcgatggt accatcacgt ggatctctag cgattacttt
    25381 taaatcctta gcaacagttt ttaaactttg gcgatgaaaa gaattaatta atgtcttatg
    25441 accgtaaata ggatataaga tagagtctgg ctcaataatc atttcgtgtg ataagaaatc
    25501 agaaggcgct tcttgccaat gtgagtcaat gtgttggtta agatttcctc ctaaggcaac
    25561 attcattaac tgtgtaccac gacaaatccc taagattggt ttttttaatg ttatagcctc
    25621 cttgatgata gctagctcaa atgtgtctcg ctctggtgaa aaatcatcat caaaagcagc
    25681 cttctcttct tgataatatt ttgggtcaac gttttgccca ccaattaaaa tgattttatc
    25741 aaccatagat acataagttt tagcggcggc ttcatcacca atgggtaaaa gaagcggcag
    25801 ccccccagat tgagtgactg cctgtacaaa tccagtaggt gcatatgacc atgggagatt
    25861 atccaaagcc atatttagtc tttgatttgc agttattcca ataatcggtt tggtcattat
    25921 acctccttaa tctagatgat tttgattcat aaaatagagt agtgtctgga gctcactggt
    25981 caaatcgaca gattgaacaa taactccttt aggaacttgt aggtgaacag gagcaaaact
    26041 caagattcct ttgattccta cttcaataag ttgatcagta acttcttgag catgaatgct
    26101 gggaaccgtt aagatagccg tttcaatgtc agtgtttgca atccgttctt tgacggatga
    26161 gataccatgg actggaatat tatcagctgt tttagtacca acaagagcgt tatcatcggt
    26221 atcaaaaccc atggcaattt gcatcttatt acgatcatgg aagcggtaat gtaatagagc
    26281 acgccctata ttaccgcatc caactaagat aacgtttgtt gtagagtgat cattaagcaa
    26341 gtcagcaaaa aaattcatca atttggtaac atcatagcca aacccacgac gccccaattc
    26401 tccaaaataa gaaaagtcac ggcgtacagt agcagagtca attcccattg catctgcaat
    26461 ttgtttagaa ctggcttttt caacttggtc agcatggaat cgtttaaaaa tacggtaata
    26521 aagtgataag cgctttgcag tcgcttttgg aatagatttg tcaataacca ctattttact
    26581 ccttgtagat ttctcgttaa atatgtctgt ctttattgta acaaaaagat tgtgaaaaat
    26641 gcaacatttt gattgttttt gtatgagtta aaaaagctaa aattagtttt agcactatat
    26701 aatagtattc attaggaaag taagttatga cagtcaaaat gaacaacatg aattaatagc
    26761 aatagaagcg aaatcattta ttgacattgt gatatgacta aaaaaacaaa atttaaagtt
    26821 gattagacga ctgttttgct aaaaatttca cgttctacaa ggctgtccat caagaaataa
    26881 aatttatctt gaaggatttg attatctttt gttttaaaaa tattaactaa tctaagacca
    26941 gattgatcgg tgatagctaa tttaaaacct gtgagttctt tattaatagt gatttttaaa
    27001 gggaaaccat cccctgaatt aggtattttt tcgagtaaac gtattaattg gtagtgccct
    27061 actttagttt gactaaatgt agtatctctt agggtatatt ttttaatgtt tggactgatt
    27121 gtataaaggt atttacagtc tttaagctta atttcttttt caaaagccat ctgttcctcc
    27181 taaaatattt tttaatgttt gagctaattg attaacatct tcaatgctat tttgatctga
    27241 aaaactgata cgaattgatt cttttaaacg agaagagtca tccccatagt acgcagctaa
    27301 aacatgactt ggattaacag ctccagcagt acaagcagaa ccagttgata ctgcaatgcc
    27361 tgctaagtca agttgtgtta gtaaaatagt attttggtag cctaaaaatc caatattgag
    27421 aacatgagga agataatgag taccttgatt aatatagtag ggtagtcctt ctaggaggct
    27481 aatcaagtga tgacgtaaac tgataatgtg atcagttgat tggtcaagac aagtcatagc
    27541 atcagtcaaa gcttgtgcca taccaataat acctaacata ttttcagtac ttgcgcgtcg
    27601 ctttccttct tgatcacccc cgtgaagaag agggtcgatt ggttgtccgt tactgtataa
    27661 aaagccgcat cctttaggac catgaaattt gtgagcagaa gcagataaaa agtcaatccc
    27721 aagttcgcta ggaataatct tgagtttgcc aacagcttga acggcatcaa catgaaaagc
    27781 agcttgatgg tctttgagca gattcccaat gtccttaata ggtaacaggt cacctgtttc
    27841 gttattggca tacattatgc ttaccaaaat ggtgtcatct ctcaaggctt gttttaagtc
    27901 agataaattt atttgtccat tttgacaagg caaataagtg acctcaaaac caaatcgctc
    27961 ttcgagatag gccatggtat gaagcacaga gtggtgctca atagtagttg tgatgagatg
    28021 tttgcccttt gcttgatggg ctaaagcata gccttttatg gccatattat tgctttcagt
    28081 tccaccggac gttactataa tttgctgttc acttgccccc aagtttctag cgatagcttg
    28141 tcggcattca cgaaggattt tattggctcg gcgcccgtag aaatgaatac tagaggggtt
    28201 accaaagtta tcttgcatag ctgctgtcat cgccctaatc acattaggac taagtggtgt
    28261 ggtggcggcg ttatcaaagt aagtcatgcg ttatttttgc ctttaggttg ataagaaaaa
    28321 agtggactta agggtcttct ttcatggatg cgaataatag cttctgcgat taaatcactg
    28381 gcgctaagat aagtaacgtt ttctggcaca cgatttttag ttttaacaga atctgtaacg
    28441 ataatttctt tgattggagc tgtttctaat acctcagcag ctccaccagc aaataagcca
    28501 tggctagcga ccgcgtaagt atcagtagcg cctgagcgtt caagaatttt agcagcttct
    28561 gcaaaggttt tcccagtatt taaaatatca tcaattaaaa tggctttttt accagaaaca
    28621 tcaccaataa tataaccttg ttcacgttca gaatcatctt gtgcatagtc gataatagca
    28681 atcggggaat caagatattc agccaagctt ctagcacgtt taattccaga atttttaggg
    28741 ctaacgacaa caacatcaga acctgataag cctaatttac tgtaacgttc tgcaaaaaga
    28801 gggactgtaa agaggttatc cactggaata tcaaaaaaac cttgtacctg aacagcatgt
    28861 aagtcaagcg tcactacacg atcaatacca gctttagtca gcatattagc tactagttta
    28921 gcagtaatcg gttcgcgggg cttagcgaca cggtcttgac gagagtaacc aaagtatggc
    28981 aatacaatat tgacggtatt agcacttgca cgtttacaag catcaatcat gatgagtaat
    29041 tcccaaagat tatcattgac aggaaaacta gtggattgaa taatatagat atcatctcca
    29101 cgcactgttt cttcaatatt gatcataatt tctccatcgg aaaattgacg tgaagacatt
    29161 tttccaagag ggattccagc agctttagca attttttcag caattggaag atttgatgtg
    29221 agtgagaaca gtttgatttg cttgtcagca tatcgttcag tcatgataga tttagctcct
    29281 taacttcaat aaaattgggt aaactgtttt attaatgagt cacattaccc aaaagtgttt
    29341 aattgacatc cctattttaa cagaaaaaca gaaaaatttc agtttttctg tcgcttttag
    29401 ccattattta tcgttgaact tttttaaggt gttaatacac cgagctactt tgcttttagc
    29461 atatttgaaa gctatgtgat gttcagataa aaaagaatca aaatcaatct ttccttgaag
    29521 agcgtcactt acttctaatt ctaattcata gtctttggta ttggcatata ggttatagtc
    29581 taaggccaat ttacctatcg gtatcactgt ctcgcgacga acggtagtta aattgccaaa
    29641 agttaccagt gcagatggtt tgataccttt tgaaataata atatcaagag cagttgattc
    29701 aggaagattc ccatgtttaa tcatatcttt tgcttgttca agaaaaagag ggacattgta
    29761 ctcacgattc cccacttttt ctggcacttt taaggttaat tcggcgctat ttgcaaatgt
    29821 tcgaatacgt aatgacattt tgttagcttt taaatcaaat gcttttgtat caatatagta
    29881 attagtttgt gtaacaggcg tcacatgttt catttgagat aaaagacggt tatactcatt
    29941 ttttgtaagt agagttttat attcgatttc taggttagtc atgggaaatt ccttttaatt
    30001 tgtgctataa taaattgtta tttcaatttt atattattat cctggctttg acaagaaagg
    30061 agacaataga tgaccttaga ttgggaagaa tttttagatc cttatatcca aacagttggt
    30121 gaattaaaaa taaaactacg gggtattcgc aagcaatatc gtaagcaaaa tcgttattct
    30181 ccgattgaat ttgttacggg acgtgttaag tcaattgaaa gtattaaaga aaaaatgatt
    30241 ttacgtggtg ttattgaaga aaatatagca caagatattc aagatattgc tggactacgt
    30301 attatggttc agtttgtcga tgatgtggaa gaagtcttag ctctattgcg tcaacgacaa
    30361 gacatgacaa ttgtttatga acgagattat attcgcaata tgaagtccag tggctacagg
    30421 tcttatcatg tggtggttga gtaccctgtt gacaccattg aaggtcaaaa aaaggtctta
    30481 gcggagattc agattcgtac tttggcaatg aacttttggg cgaccattga gcattcttta
    30541 aattataaat atggtggaga cttcccagaa gaaattaaaa agcgtcttga agtgactgct
    30601 aaaattgctt tagaattaga cgaagaaatg cgtaagattc gagaagatat tcgagaggct
    30661 caattattgt ttgaccccgt gacacgaaat ttaagtgatg gtgtaggaaa tagtgatgac
    30721 acagatgaat tatacaggta aggtaaagcg agttgctatt attgcaaatg gtaagtacca
    30781 aagtaaacgc gttgcctcca aacttttctc cgtatttaaa gatgatcctg atttctatct
    30841 ttcaaagaaa aatccggata ttgtgatttc tattggcgga gatgggatgc tcttatctgc
    30901 ttttcacatg tatgaaaaag aattagataa ggtacgtttt gtaggaatcc acaccggtca
    30961 tcttggcttt tataccgatt atagggattt tgaagttgat aaattgattg ataatttaag
    31021 aaaagacaag ggagaacaaa tctcttatcc gattttaaaa gttgctatta ctttagatga
    31081 tggtcgtgtg gttaaagcgc gtgctttgaa tgaagcgacg gttaagcgta ttgaaaaaac
    31141 gatggtagca gatgttatta ttaaccatgt caaatttgaa agcttccgag gtgatgggat
    31201 ttcagtatcg accccgacag ggagcacagc ctacaataaa tctttaggtg gtgctgtctt
    31261 gcatccgacg attgaagcgc tgcaattgac ggaaatttcc agtcttaata accgtgtctt
    31321 tagaaccttg ggctcatcaa tcattattcc caaaaaagat aagattgagt tagtgccaaa
    31381 acgattagga atttatacca tttccattga taataaaacc tatcagttaa aaaatgtgac
    31441 gaaggtggag tattttatcg acgatgagaa aattcatttt gtttcctctc cgagtcatac
    31501 gagcttttgg gaaagggtca aggatgcctt tattggagag actgactcat gagatttgaa
    31561 tttgtagcag acaaacgaat aaaagtaaaa acccttttaa aaagtcatga tgtctctaaa
    31621 ggactattgg ctaaaatcaa atataagggc ggtaatatat tagtgaatgg tattgaacaa
    31681 aatgccattt atttattgca agtgggagat gttgtcacca ttgacattcc aaacgaagag
    31741 ccctttgaaa agcttgaagc catccctttt gatttggata ttgttcacga ggatgatcat
    31801 tttttggtga ttaataaacc cattggcttt gccagtattc caagtgccat ccactccaat
    31861 accattgcta actttatcaa ggcatattat gtggacaatc attacctaga ccaacaagtg
    31921 catatcgtga ctcgtttaga cagagatacg agtggcttga tgttatttgc taagcatggt
    31981 tatgctcatg caaggttgga caagcaactc caaactcgct caatagagaa acgttatttt
    32041 gctctggttt ctggaaatgg gacgttgcca gatgaaggag atattattgc tccaattggg
    32101 cgttcaaaag atagtattat taccagggca gttgacccta tgggcaaata tgccaagact
    32161 agctataagg ttgtggcgcg ctattcagaa aatgtgcatt tagtggatat caaattgcat
    32221 acaggacgta cccatcaaat cagagttcat tttgcccata ttggttttcc acttttaggg
    32281 gatgaccttt acggaggtcg tttggactta ggaataacaa ggcaagcttt acattgtcat
    32341 tatttgaatt tcaaagatcc atttacagaa agtgattgta gctatgcaat acacttgaca
    32401 gatgactttg atagcgttat cataggttta cagaaaaaag atagaggtta ttaaagaatg
    32461 agtattcgta gtttattcgg tggtttaaga gaaaaaattc ttggtaaaaa catgaagatt
    32521 gttttcccag aaggtaatga tgagcgcgtc gtccgtgcag ctgctcgttt gaaatttgaa
    32581 ggactcttag aaccgattat ccttggacag tcagaagagg tacgcaacct tttgacaaaa
    32641 ttaggtttcg ctgatcaaga ctatactatc attaatccaa atgaatatgc ggattttgac
    32701 aaaatgaaag aagcttttgt tgacatccgt aaaggaaaag caacgcttga agatgctgat
    32761 aaaatgcttc gtgatgtgaa ttactttggt gttatgttgg ttaaaatggg attagcagat
    32821 ggaatggtat ctggtgctat tcattcaaca gctgatactg ttcgtccagc ccttcaaatc
    32881 attaaaacca aaccaggtat ttcaagaact tcaggtgttt tcttgatgaa tcgcgaaaat
    32941 acgagtgagc gttacgtgtt tgctgattgt gctatcaata ttgacccaac agcacaagaa
    33001 ttggcagaaa tcgctgttaa cacagctgaa acagctaaaa tctttgatat tgatcctaaa
    33061 attgctatgt taagcttctc aacaaaaggt tctggtaaag cgcctcaagt tgataaggta
    33121 agagaagcaa cagaaattgc taaagggttg aaccctgatt tggcacttga tggtgagttg
    33181 caatttgatg cagcctttgt tcctgaaaca gcagctatca aagcaccaga ttcagcagtt
    33241 gctggtcaag ctaatacttt cgttttccct gacttacaat ctggaaatat tggttacaaa
    33301 attgcacaac gtttaggaat gtttgatgct attggaccaa tcttacaagg attgaacaaa
    33361 cctgttaatg atttatctcg tggctcaagc gctgaagata tttacaaatt agctattatt
    33421 acagcagcac aagctattga aagccaaggg taacttaaga ccccttgata taggattcta
    33481 accgctaagt gatgtgttag aagttgtcct aagactaatt gacattatta aaaacgagtt
    33541 ggccagacca tctcgttttt ttgaaggtga ggaaatgtat gggaaatatt gccttagtaa
    33601 caggggcttc agccggtttt ggcagagcca ttgtcgaaca cttaattgca gatgggataa
    33661 aggttattgg tgccgctcgt cgtcttgaga aactcaaggc cttagaaacg agctttgggt
    33721 cggacaactt ttaccctttg cagatggatg tcacagatac caaagcgatt gatgatgctt
    33781 tgacaagtct accaaatcag tggcaagata ttactatttt agtgaataat gcaggcttgg
    33841 cattgggact tgataaggct tatgaagccg attttgagaa ttggatgacg atgattaata
    33901 ccaatattgt tgggttgatt tatctcacac gtcaattatt accacatatg gtctccaaag
    33961 atgctggtat tattattaat ttagggtcaa ctgcagggac aattccctat ccgggagcta
    34021 atatttatgg ggctagcaag gcttttgtta agcagttctc actcaattta cgagctgatt
    34081 tagcaggtag ccatatacgt gtgaccaata ttgaacccgg tctatgtgaa gggacagaat
    34141 tttcaaaggt tcgctttaat ggtgatgagg cacgggtgaa ggccatttat caaggagcac
    34201 atgccattca acctcaagat attgcgtcga ctgtatcatg ggtgattcat cagccccctc
    34261 atgttaatgt taatcgtatt gagctgatgc cagtgtcgca aagttatgga ccgcaaccag
    34321 ttagtcgtga ctagcaaggg atgagtccat aagaataata atctttagaa aaatggttta
    34381 ctggtatgga ttattcttta taagtgagac ttaccgattt agtcatcaaa aaaagcaatc
    34441 aagaagaagg ttttgaggtc ttcaagcgtt catgattgct tcttgtgcgt tgatgattta
    34501 ggctgaactc cctatttaaa atcaagttta gtgatctaaa atggaagggc ctgatgtgac
    34561 atcggaccag ccaacaatgg cttgtccagt ttcttctaaa tactggttta ataacggttt
    34621 aatgtcttga acggtatcca aaactcccac cagctgacct tgaggatttt taagagcttg
    34681 ataatgctgc atgtaaatat gatccaatga acccgaagga agtaggaagg ttttacaagg
    34741 gctatctgat aaacgcaatt cagcaagaat ccaatcttct atttcgttat cagtagttgg
    34801 caaaaaactt ccattaaaag catgattatc gactagaatg ttcccctttg catcagataa
    34861 ccttgcttgc aaaggaaggt actgtaaaaa atatccatca aatttttggt aaaatctagt
    34921 gaggtattgg cttaacaata agactgttgt tgcttgatca aagacacttt ctgtcactaa
    34981 aaagccctga ccatgttgag aggttttgaa atagagtgta ggcaagtcaa tgtcatgctc
    35041 atggaaactg aaaattcctt ggaaagtcga caattgattt gactgaatgt tggctttgct
    35101 agtgagaata gtcacctcaa aaaggtgtga gaagtggtgg gaaagctctc taataaacgt
    35161 atcttcaagt tggttttcag gtgttaagag cgctaaaatg gaaatggcat ttttcataat
    35221 tgtatgatag ctgtattttc tagaaaagac aattaatctg cttatctatc atcataggca
    35281 agtctaggat ttttgataag gtaattttgg agagataggc tgacaaagtg acctgttttt
    35341 ctataagggg taaggaggaa tagtctcatt atttgactta tagtgggaaa ctttttataa
    35401 tggtcaacaa caaacgacaa gaaaataatg agagaaaatg tttttgagaa ttggtagttt
    35461 ttaccttact gaggatacct aagagtccct ttaattagta gatgcgtttg tctaaaaatg
    35521 gaagtgcctg cagtaattcc ttgtagatac ttagaacgtt ttgagaaaaa atgacagaga
    35581 tacaggttga atgaactcat cagcttttat gctgttgctt taaccaatga attggagaga
    35641 taatgatacg ttttaataat gtttctaaaa cttttggtca gacaaaggtt ttgcaagagc
    35701 agacctttca aattaatgac cgtgaatttt ttgtgctggt tggccccagc ggatcgggca
    35761 agaccacact tttaaaaatg attaattgcc ttattgaacc aagctctgga gatattttac
    35821 taaataacgt cccacagaaa gagttagatt tacgtgagat gaggctatct attggatacg
    35881 tattgcagca aattgctctg ttccccaatc tcacagtagc tgaaaatatt gcgattatcc
    35941 ctgagatgaa gcaatggtca gctgaagaaa ttagacaaaa aactgaagaa ttattggata
    36001 aggtgggcct acctgctaaa gattatcttg atcgttaccc aagtgattta tcgggaggtg
    36061 aacagcaacg tattggtatt gtacgtgcta tcatttctca tcctaaaatt ctattgatgg
    36121 atgaaccatt ttcagcctta gatcctattt cacgtaaaca attgcaagag ttaatgttga
    36181 gtcttcacaa ggaatttgac atgacgattg tatttgtgac acatgatatt gatgaggcca
    36241 ttaaattagg agaccgtgta gctattttaa atgagggaga aattgtgcaa ctggatcgcc
    36301 cagaaatgat taagacccac cctgctaatg cttttgtggt caatttattt ggaggtgatg
    36361 agcatgccta gtttgtttgt aactttccaa aaccgtttta atgaatggct ggcggctctg
    36421 ggggaacacc tgcaaatttc ccttttatct cttatgatcg ccttgctaat aggtgtgcct
    36481 ttagcagccc ttctcagtcg cagcaaacgt tggtcagaca ttatgttaca ggtaacaggt
    36541 gtttttcaaa ccattccctc actggctttg cttggtcttt tcatcccttt aatgggaatt
    36601 ggaacgttgc ctgcagtgac agctttagtt atctatgcga tttttccgat tttacaaaac
    36661 accatcacag gattaaatgg tattgaccca agtctcgtgg aagcaggaat agcttttggg
    36721 atgaccaaat gggagcggtt aaaaacgttt gagattccaa ttgcaatgcc tgttattatg
    36781 tcaggtgtgc ggacgtcagc agtcatgatt atcggcacag ctactttagc ttccttgata
    36841 ggagccggtg gacttggctc tttcatctta ttggggattg atcgtaataa tgctaacctg
    36901 attctgatag gggctatttc ttcagctctg ctagctatta ttttcaatag tttgttacag
    36961 taccttgaga aagcttcctt gcgacggatt atgattagtt ttggaatcac cttacttgca
    37021 ttgctagcat cgtatactcc tatggcgctt agtcagtttt caaaaggaaa agatacagtg
    37081 gttattgccg gtaaattggg agcagagccc gatattctga ttaatctcta taaggaatta
    37141 attgaagacc aatcagatat aagtgttgag ttgaaatcta attttgggaa aactagcttc
    37201 ttatatgaag cccttaaatc tggagatatt gatatgtatc ctgaatttac aggaaccata
    37261 acatcaagtc ttttacgcga caaaccacct ttgtctaatg accctaagca ggtctatgag
    37321 gatgctaaaa aaggcattgc taagcaagat aaactgaccc ttctcaagcc atttgcttac
    37381 caaaatacgt atgctgttgc tatgccagaa aaattggcta aggaatatca gattgaaacc
    37441 atttctgatt taaaagcgca tgctgatact ttaaaggctg gttttacttt ggaatttaag
    37501 gacagagcag atggctataa gggaatgcaa tctcaatatg gattacagct atctgtggcg
    37561 acgatggagc cagctcttcg ttatcaagca attcaatcag gagatatcca agtaacagat
    37621 gcttactcta cggatgctga aattacgaaa taccatttga aagttttaaa ggatgataaa
    37681 cagttgtttc caccttatca gggagctcct ttgatgaaaa cttccttatt aacaaaacat
    37741 ccagaattaa aaggcattct taatcaatta gcgggaaaaa ttactgaaaa agagatgcag
    37801 gacatgaact acgaggtatc tgttaaagga gcagatgcta ataaggtcgc tcgagattat
    37861 ctgttgaaaa caggcttaat ccagaaataa acagaaatgt tggtccgtag gactggcatt
    37921 ttttcgtctc ttaagttcaa gaagtaacaa ttcataaaca caaaaaccga
//
LOCUS       DAUDYX010000014        37850 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.014, whole genome shotgun sequence.
ACCESSION   DAUDYX010000014 DAUDYX010000000
VERSION     DAUDYX010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37850
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.014"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            141..1337
                     /gene="tuf"
                     /locus_tag="VHD02_000679"
     CDS             141..1337
                     /gene="tuf"
                     /locus_tag="VHD02_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEQ5497310.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETATRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDTKFEDIIMELMDTVDSYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEIEIVGIKEETKKAVVTGVEMFRKQLDEGLAG
                     DNVGILLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILSKDEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTINVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
     gene            1578..2336
                     /locus_tag="VHD02_000680"
     CDS             1578..2336
                     /locus_tag="VHD02_000680"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEQ5497311.1"
                     /translation="MSRKPIIAGNWKMNKNPQEAKAFVEAVASKLPSTDLVDVAVAAP
                     AVDLVTTIEAAKDSVLKVAAQNCYFENTGAFTGETSPKVLAEMGADYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGLTPIVCCGESLETYEAGKAVEFVGAQVSAALAGLSAEQV
                     ASLVLAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVKDYMACPDVDGALVGGASLEADSFLALLDFLN"
     gene            complement(2435..3670)
                     /locus_tag="VHD02_000681"
     CDS             complement(2435..3670)
                     /locus_tag="VHD02_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEQ5497312.1"
                     /translation="MALIEISQEQFDHYCHSLVHHSFIQTSEMASLMAKKGAKPQFLG
                     LEKDGELKVAAMVFSQKVAGGWRMELNAGPNTNHPEELEHFYTQLKDYAKQKDVIELI
                     LKPYDNYQSFDTDGIPISHPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGI
                     DSSRLTHSFSKKGKALIKKANTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSY
                     YQDFYDSFGDSCEFMVATLNFEDYLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNR
                     LKELSSQFETFQVRISEALHFLEEYGTKDVFLAGSLFIYTEQEAVYLFSGSYPKFNKF
                     YSPALLQEHAMLKAIHKGIKQYNFLGITGKFDGSDGVLRFKQNFNGFILQKPGTFRCY
                     PFPIKYHFIRLAKKLLNRY"
     gene            complement(3657..4883)
                     /locus_tag="VHD02_000682"
     CDS             complement(3657..4883)
                     /locus_tag="VHD02_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HEQ5497313.1"
                     /translation="MYTYKIGISPEEHDQFVLAQPQAGLLQSSKWGKVKNNWKHERIS
                     FYENGVQVAAAACLIRKLPLGFTMIYIPRGPIMDYANFELLDFVIKTLKTVGKSKRAL
                     FVKIDPSLVIKQTLEGKESKENDVTLSIIAFLKKLGVEWSGRTKELEDTIQPRIQANI
                     YAKDFDFDSLPKKAKQSIRTATNKGVNVTIGGSELLDDFSALMKKTENRKGIILRGKS
                     YYQKLLDIYAGQSYITMASLDLPEQKKLLIQQLDKALAEQARLTDKSKPSKVAENQKT
                     IARLQKDLTILSEQLATGQTRIPLAATLTLIYGETSENLYAGMDDDYRNYQAPLLTWY
                     KTAKEAFKRGCRWHNLGGVENQQDGGLYHFKARLNPTIEEFAGEFNIPVGLVSSLAIL
                     TYNLRKKLRSTTNGTN"
     gene            complement(4883..5692)
                     /gene="yidA"
                     /locus_tag="VHD02_000683"
     CDS             complement(4883..5692)
                     /gene="yidA"
                     /locus_tag="VHD02_000683"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEQ5497314.1"
                     /translation="MSIKLVAVDIDGTLLTDDRRITDDVFQAVQEAKAQGVHVVIATG
                     RPIAGVISLLEQLELNHKGNHVITFNGGLVQDAETGEEIVKELMTYDDYLETEFLSRK
                     LGIHMHAITKEGIYTANRNIGKYTVHESTLVNMPIFYRTPEEMTNKEIIKMMMIDEPD
                     LLDAAIKQIPQHFFDKYTIVKSTPFYLEFMPKTVSKGNAIKHLAKKLGLDMSQTMAIG
                     DAENDRAMLEVVANPVVMENGVPELKKIAKYITKSNNDSGVAHAIRKWVLN"
     gene            complement(6148..7449)
                     /locus_tag="VHD02_000684"
     CDS             complement(6148..7449)
                     /locus_tag="VHD02_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEQ5497315.1"
                     /translation="MNEKVFRDPVHNYIHIDNPLIYDLINTKEFQRLRRIKQVPTTAF
                     TFHGAEHSRFSHCLGVYEIARRVTAIFEEKYADIWNKDESLVTMTAALLHDIGHGAYS
                     HTFEALFHTDHEAFTQEIITNPETEINAILVRHAPDFPDKVASVINHTYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYFSAANYGQFDLMRILRVIRPVEDGIVFEHSGMHAVEDYIVS
                     RFQMYMQVYFHPASRAVELILQNLLKRAQHLYPEQQAYFQKTAPGLIPFFEKKANLAD
                     YIALDDGVMNTYFQVWMASEDHILSDLASRFINRKILKSVTFDQDSQGELERLRQLVE
                     SVGFDPDYYTGIHINFDLPYDIYRPELENPRTQIEMIQKDGSLAELSQLSPIVKALTG
                     TTYGDRRFYFPKEMLELDDLFAPSKETFMSYISNGHFHFSQ"
     gene            7532..7918
                     /locus_tag="VHD02_000685"
     CDS             7532..7918
                     /locus_tag="VHD02_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEQ5497316.1"
                     /translation="MKLQLTNHIRFGDETEIIQEIHDCEWREKGGYQYLIYQNTDKEK
                     VVIKYNETELTMSRFSNPQSIMKFFAGKKVLIALPTPMGVQQFLTDTSHYHLDCSCQK
                     LDLHYHLLQAQTEMLFASYHLELSWD"
     gene            8148..10829
                     /locus_tag="VHD02_000686"
     CDS             8148..10829
                     /locus_tag="VHD02_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEQ5497317.1"
                     /translation="MSKEQRHEAFYTQSEETVLAQLETSREGLTSAQAKERLAEYGRN
                     ELDEGEKRSLFMKFLDQFKDLMIIILIVAALLSVLTEGMEGLTDAIIILAVVILNAAF
                     GVYQEGQAEAAIEALKSMSSPLARIRRDGHVTEIDSKELVPGDIVLLEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSTAVSEDAGIGDRVNMGYQNSNVTYGRGIGVIT
                     NTGMYTEVGHIAGMLSNADETDTPLKQNLDNLSKILTYAILVIAAVTFAVGVFLRGQH
                     PLEGLMTSVALAVAAIPEGLPAIVTVVLSLGTQVLAKRNAIIRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGTLQSSSADIAFDNTTLRVMNFANDTKVDPSGKLIGDP
                     TETALVQFGLDHNFDVREAMVAEPRVAELPFDSDRKLMSTIHKQADGKYFIAVKGAPD
                     QLLKRVTQIEENGQIRPITDADKKTILDTNKSLAKQALRVLMMAYKYSDALPTLETEI
                     VEANLVFSGLVGMIDPERPEAAQAVKVAKEAGIRPIMITGDHQDTAKAIAKRLGIIEE
                     DGVDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNEGKVVGMTGDG
                     VNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKTI
                     QYLLSANMAEVFTIFLATLFGWDVLQPVHLLWINLVTDTLPAIALGVEPAEPGVMKHK
                     PRGRKSSFFDGGVKEAILYQGAFQTILVLGVYGFALMFPEHTSYHDVHADALTMAYVT
                     LGLIQLVHAYNVKSVYQSIFTVGLFKNKLFNYSIPVAFVALMATVVVPGFNQFFHVTH
                     LTITQWLVVIIGSLLMVVLVELVKAVQRSLGQDEKAI"
     gene            complement(10913..11908)
                     /locus_tag="VHD02_000687"
     CDS             complement(10913..11908)
                     /locus_tag="VHD02_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ5497318.1"
                     /translation="MQRKVTIKDIAELAKTSKTTVSFYLNGKFDKMSEETKNRIKSTI
                     EATNYKPSIAARSLNAKSTKLIGVVIGDITNSFSNQIVKGIESKAQEFGYQIIIGNSN
                     YDPSREDELIEKMLNLGVDGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWV
                     KTNNYDAVYDTIQQCIDKGYEHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMI
                     IDENQTSSEAIAQFLQGSLTKKSLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNI
                     EWTKFSSPTLTTIIQPAYEEGEQATKILIDDIEGHSQEAKQQIFDCQVNWQESTF"
     gene            complement(11972..13878)
                     /locus_tag="VHD02_000688"
                     /pseudo
     CDS             complement(11972..13878)
                     /locus_tag="VHD02_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995224.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
     gene            complement(13965..14786)
                     /locus_tag="VHD02_000689"
     CDS             complement(13965..14786)
                     /locus_tag="VHD02_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEQ5497319.1"
                     /translation="MTGSNKLTKKDYLKTALRAFFLQNGFNYSNYQGIGYANVIYPAL
                     KKHFGSDKKGLYQALEDNCEFYNTNPHFLPFITSLHLVMLENDRPEEETRNIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLASDGLVLGPIFFFLAMNIILTAIKIGSGLYGYK
                     VGTSFIDKLSEQMAVVSRMANIVGVTVIAGLAATSVKITVPITFAAGKVDAANTAQKF
                     VTIQGMLDKIAPALLPALFTLLMYYLIKNKKWTTYKLVLLTVLIGIIGSWLGILA"
     gene            complement(14773..15555)
                     /locus_tag="VHD02_000690"
     CDS             complement(14773..15555)
                     /locus_tag="VHD02_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEQ5497320.1"
                     /translation="MDINLLQALLIGLWTAFCFSGMLLGIYTNRCIILSFGVGIILGD
                     LPTALSMGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVTPEAALALST
                     PIAVVIQFLQTFAYTAFAGAPETAKKQLQKGNIRGFKFAANGTIWAFAFIGLGLGLLG
                     ALSMDTLLHLVDYIPPVLLNGLTVAGKMLPAIGFAMILSVMAKKELIPFVLIGYVCAA
                     YLQIPTIGIAIIGIIFALNEFYNKPKQVDATTVQGGQQDDWI"
     gene            complement(15574..16062)
                     /locus_tag="VHD02_000691"
     CDS             complement(15574..16062)
                     /locus_tag="VHD02_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEQ5497321.1"
                     /translation="MTQPNIIMTRVDERLIHGQGQLWVKFLNCNTVIVANDAVSEDKI
                     QQSLMKTVIPSSIAIRFFSIQKVIDIIHKASPAQNIFIVVKDLQDAKRLVEGGVPITE
                     INIGNIHKTDDKVAITQFISLGETDKSAIRCLAHDHHVVFNTKTTPAGNSASDVDILD
                     YI"
     gene            complement(16098..17297)
                     /locus_tag="VHD02_000692"
     CDS             complement(16098..17297)
                     /locus_tag="VHD02_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEQ5497322.1"
                     /translation="MTRSLKTIALEPIKQPERFTKEDFLSQEDITQALDLALKQVRLN
                     MDYFKEDFPTPATKDNQYAIMDNTEWTNAFWTGCLWLAYEYSGDDAIKALAQANDLSF
                     LDRVTRDIELDHHDLGFLYTPSCMAEWKLLKTPEAREAALKAADKLVQRYQDKGGFIQ
                     AWGELGKKEDYRLIIDCLLNIQLLFFASQETGDNRYRDMAINHFYASANHVIRDDASA
                     YHTFYFDPETGDPVKGVTRQGYSDDSAWARGQAWGIYGIPLTYRFLKEPELIQLFKGM
                     THYFLNRLPKDQVSYWDLIFRDGSGQSRDSSATAIAVCGIHEMLKTLPDHDPDKKTYE
                     AAMHSMLRALIKDYANKNLKPGAPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALLRFY
                     KDWNPYW"
     gene            complement(17297..17734)
                     /locus_tag="VHD02_000693"
     CDS             complement(17297..17734)
                     /locus_tag="VHD02_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ5497323.1"
                     /translation="MIAIIVMGHGHFASGIVSSLELIAGPQEKVTAIDFTAEMTAADV
                     QDQLSRALIPEEETLVLCDLLGGTPFKVAATLMESLPNTTCNVLSGLNLAMLIEASFA
                     RQTAASFDDLVSGLITCSKEGIVDWKTLSQQEDGATDDELGGI"
     gene            18059..18853
                     /locus_tag="VHD02_000694"
     CDS             18059..18853
                     /locus_tag="VHD02_000694"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEQ5497324.1"
                     /translation="MENMFSLQGKIALITGASYGIGFEIAKAYAQAGATIVFNDIKQE
                     LVDKGLAAYRELGIEAHGYVCDVTDEAGIQQMVSQIEDEVGVIDILVNNAGIIRRTPM
                     LEMAAEDFRQVIDIDLNAPFIVSKVVLPSMIAKGHGKIINICSMMSELGRETVSAYAA
                     AKGGLKMLTKNIASEFGEANIQCNGIGPGYIATPQTAPLRERQADGSRHPFDQFIIAK
                     TPAARWGTTEDLAGPAVFLASDASNFVNGHILYVDGGILAYIGKQP"
     gene            18878..19519
                     /locus_tag="VHD02_000695"
     CDS             18878..19519
                     /locus_tag="VHD02_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEQ5497325.1"
                     /translation="MKIALINENSQAAKNGIIYDALTSVTDKHGYQVFNYGMYGTEGE
                     SQLTYVQNGLLASILLTTKAADFVVTGCGTGVGAMLALNSFPGVTCGFASEPTEAYLF
                     SQINGGNALSIPFAKGFGWGAELNLTLIFERLFAEPMGGGYPKERAIPEQRNARILSD
                     LKKITYRDLLAIVKDIDQDFLKETISGAHFKEYFFANAEPSELVAYLKSVLEQ"
     gene            19548..20549
                     /locus_tag="VHD02_000696"
     CDS             19548..20549
                     /locus_tag="VHD02_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEQ5497326.1"
                     /translation="MSKLLLVGEPLIRVSPNQFQPLTNACDAQLFFGGSEVNIARTLG
                     GFGLEARLFTALPDNPVGHAFHQFLKQSGVDMTLTAWQGHRVGLYYLENGFGCRASQV
                     YYDRCGSSFSALDKDSLDLAAIFEGIRHFHFSGISLALGKKTQDLIEILAREAKKRDI
                     CISFDLNFRSSMIAVADAKRLFSHFAQYADIIFGMEPLLLDSDDFDMFDRNKADTTTI
                     RERLAGLYQRYQLQAIYHTERSNDAQGSNRFKAYAYDGQFYESCEVTTPVLQRVGSGD
                     AFVAGLLYQLLEGNEKQRNLDFAVATASLKCTVAEDQLYHSVQQVEAVLHNQRDVQR"
     gene            20554..21189
                     /locus_tag="VHD02_000697"
     CDS             20554..21189
                     /locus_tag="VHD02_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEQ5497327.1"
                     /translation="MGKIEILTKLKANRLVLVVRGESSEEALACSLASIEGGIKTIEV
                     TYTNPFASEVIGQLAERFKEDPEVLIGAGTVLDDVTARQAILAGAQFIVGPNFNRAVA
                     LICHRYSIPYLPGCMTVNEVVTALESGVDMVKIFPGSTVGMSFIRAIKSPLPQVEVMV
                     TGGVSSDNLKDWLAAGVDVLGIGGEFNQLASQKQYNLITKKAAHYIKSLRQ"
     gene            21486..22136
                     /locus_tag="VHD02_000698"
     CDS             21486..22136
                     /locus_tag="VHD02_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEQ5497328.1"
                     /translation="MIKGIIFDMDGVLFDTEPFYLRRREDFFKTKGIPIDHLNSKDFI
                     GGNLQELWKELLGKNRDDAIVKAITTDYDAYKQAHKPPYQKLLITEVNSCLEQLKKQG
                     IKLAVASNSKRQDVLLALETTQIKDYFEIILAREDVSRGKPYPDIYNKAVQKLGLQKK
                     QLLVVEDSQKGIAAAKAANLTVFAITDYRYGIDQSQADHKIDHLGQLCVKIGCFGS"
     gene            22834..23958
                     /gene="queG"
                     /locus_tag="VHD02_000699"
     CDS             22834..23958
                     /gene="queG"
                     /locus_tag="VHD02_000699"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEQ5497329.1"
                     /translation="MTIKAEIKALAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVIEDRISPERLLESAKTIISIGVAYPHKLPQQPQKTPYKRGKITPSSWGFDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCIDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNPPATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGSWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEILAFMSHLTLKDEDSQKELELIRHKWQF"
     gene            24027..25125
                     /gene="prfB"
                     /locus_tag="VHD02_000700"
     CDS             join(24027..24098,24100..25125)
                     /gene="prfB"
                     /locus_tag="VHD02_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEQ5497330.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENHMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            25144..25836
                     /gene="ftsE"
                     /locus_tag="VHD02_000701"
     CDS             25144..25836
                     /gene="ftsE"
                     /locus_tag="VHD02_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEQ5497331.1"
                     /translation="MALIEMSGVTKKYRRSTTALRDVNVSVNQGEFVYLVGPSGAGKS
                     TFIKLLYREEQLTTGKLYVGEFNLTKLKARDVPILRRHIGVVFQDYKLLPRKTVFENV
                     AYAMEVIGEKRRHIKKRVPEVLDLVGLKHKMRSFPSQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEISWEIMQLLERINVQGTTILMATHNSHIVNTFRHRVVAIEDGRIV
                     RDEEKGDYGYDD"
     gene            25829..26758
                     /locus_tag="VHD02_000702"
     CDS             25829..26758
                     /locus_tag="VHD02_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ5497332.1"
                     /translation="MIRYFFRHIWESIKNLKRNFWMTFASVSMVAVTLTLVGVFAATL
                     LNIQRVASGVENNVHINTYLQVDSTDAAKVIQNTAGEPVNNDNYHSVYDKIAQIKGVK
                     KITFSSKDEQLKKLQETLGDVWNMYDQDTNPLQDIYLIETQTPKQVKAITKKIRTIEG
                     VEAADYGGINSDKLFKFSTLIQTWGLIGTAMLLFVAVFLISNTIRMTIMSRKRDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGLLGAVLPSLLIYYGYDLVYKHFAQELQRNNLSMYPL
                     DPYVYYLIGALFVIGIMIGSLGSVLSMRRYLKF"
     gene            complement(27067..27765)
                     /locus_tag="VHD02_000703"
     CDS             complement(27067..27765)
                     /locus_tag="VHD02_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEQ5497333.1"
                     /translation="MPFIFRYSFFNKVLIFWYTILMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDAFDHPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIITVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSLGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPNHYRVYPGHGPS
                     TTICHEKNANPFFH"
     gene            27933..28697
                     /locus_tag="VHD02_000704"
     CDS             27933..28697
                     /locus_tag="VHD02_000704"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEQ5497334.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHADGFKIGILDYNEETAQAAVD
                     KLSPEDTVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGVAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIAHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLAGDDSNYITGQTIVVDGGMVFH"
     gene            28805..31306
                     /locus_tag="VHD02_000705"
     CDS             28805..31306
                     /locus_tag="VHD02_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEQ5497335.1"
                     /translation="MFCFIDIACYNRLTMTQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLAKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEALFLEGYELTIPRVDTVELAQLFFPRFEKYNLGHLSRQLNIDL
                     AEAHTAIADARATAILFLRLLQKIESLPIECLESLLVYSDSLLFETAMVIQEGLAKAK
                     PYDPNKYIKIRQILLPKGSKALKPYQISKSFPINMALLGLEERPKQTQFAQLIDEDYH
                     QGVASFIEAQTGIGKTYGYLLPLLAKEDQNQIIVSVPTKLLQDQLMAGEVAAIQEQFH
                     IACHSLKGPANYLKLDSFADSLDQNDQNRLVNRYKMQLLVWLLETKTGDLDEIKQKQR
                     FAAYFEQLKHDGDIKQSSEFYDYDFWRVSYEKAKTARLLITNHAYFLHRIQDDKDFTR
                     NKVLVFDEAQKLMLQLDQLSRHQLNLTVFLQTIQAKLSNPLPLLEKRLLESLSFELGQ
                     VSSDYYQNKEHQLAHDWSRIAGYAKELTGADYQELQAFFATSDGDYWLSSEKQEEKRV
                     TYLNSASKAFIHFQQLLPETVKTYFVSATLTISSEVTLADLLGFEEYLYHVIEKDKKQ
                     DQLVLVDQEAPIVTEVSDQIYVEAIAKRIESLKQEGYPILVLFNSKKHLLLVSDYLDQ
                     WQVPHLAQEKNGTAYNIKKRFDQGEQTILLGLGSFWEGVDFIQADRMITLIARLPFDN
                     PEDFFVKKMSHYLLEKGKNPFRDYFLPMTILRLKQAIGRTMRRKDQKSVVIILDRRLL
                     TKSYGQVILEGLGQEFLISQQNFHDCLVETDCFLI"
     gene            31641..32834
                     /locus_tag="VHD02_000706"
     CDS             31641..32834
                     /locus_tag="VHD02_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEQ5497336.1"
                     /translation="MPKLSKRVLEMKESVTLAAGARAKALKAQGHDVLNLTLGEPDFF
                     TPKHIQDKAIESIQNGTASFYTNASGLPELKAAIATYLKNQYGYHLSPDQIVAGTGAK
                     FILYAFFMAVLNPGDQVLIPTPYWVSYSDQVKMAEGQPIFVQGLEENQFKVTVDQLER
                     ARTSKTKVVLINSPSNPTGMIYGAEELRAIGEWAVHNDILILADDIYGSLVYNGNQFV
                     PISTLSEAIRRQTITVNGVAKSYAMTGWRVGFAAGEPEIISAMSKIIGQTTSNLTTVS
                     QYAAIEAFCGSQSSLEEMRLAFEERLNITYPLLCQVPGFEVVKPQGAFYFFPNVKKAM
                     EMTGFSDVTSFANAILEEVGLAVVSGAGFGAPENVRLSYATDIETLKEAVRRLHVFME
                     SNEIN"
     gene            32855..34201
                     /gene="asnS"
                     /locus_tag="VHD02_000707"
     CDS             32855..34201
                     /gene="asnS"
                     /locus_tag="VHD02_000707"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEQ5497337.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVTNKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEIIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYATYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYITEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIRP"
     gene            34616..35506
                     /gene="rapZ"
                     /locus_tag="VHD02_000708"
     CDS             34616..35506
                     /gene="rapZ"
                     /locus_tag="VHD02_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEQ5497338.1"
                     /translation="MSDKHINLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALVPK
                     FLELIEQTNENRRVALVVDMRSRLFFKEINSTLDSIESNPSIDFRILFLDATDGELVS
                     RYKETRRSHPLAADGRVLDGIRLERELLSPLKSMSQHVVDTTKLTPRQLRKTISDQFS
                     EGSNQPSFRIEVMSFGFKYGLPLDADLVFDVRFLPNPYYQVELREKTGLDEDVFNYVM
                     SHPESEVFYKHLLNLIVPILPAYQKEGKSVLTVAIGCTGGQHRSVAFAHCLAESLATD
                     WSVNESHRDQNRRKETVNRS"
     gene            35503..36480
                     /locus_tag="VHD02_000709"
     CDS             35503..36480
                     /locus_tag="VHD02_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEQ5497339.1"
                     /translation="MKNPKMTVIGGGTGISIILKSLRNEAVDITAVVTVADDGGSSGE
                     LRNAMQLAPPGDLRNVLLAMSDMPKFYERVFQYRFNESDGALAGHPLGNLIIAGISEM
                     QGSTYNAIQILTKFFHITGKIYPSSEQALTLHAVFKDGHEVAGESSIAKYPGMIDHVY
                     VTNTYNDQKPQASRKVVEAILESDMIVLGPGSLFTSILPNLVIPEIKEALRQTKAEVV
                     YICNIMTQYGETEQFSDADHVAVLNQHLGRDLIDTVLVNVAKVPQAYMNSNKFDEYLV
                     QVDHDFAGLCRAAKRVISSYFLRLENGGAFHDGNLVVEELMNLVRIVKQ"
     gene            36477..37388
                     /gene="whiA"
                     /locus_tag="VHD02_000710"
     CDS             36477..37388
                     /gene="whiA"
                     /locus_tag="VHD02_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEQ5497340.1"
                     /translation="MSFTTKVKEELIHLSTGDNNELAAIIKLSGSLGLAHQSLHLSIT
                     TENAKIARYIYSLIEDAYVIVPEIRYHQKTNLRKNRVYTVYVEQGVETILADLKLADS
                     FFGLETGIEPQVLSDDNAGRSYLKGAFLAAGSIRDPESGKYQLEIYSVYLDHAQDLAQ
                     LMQKFMLDAKTIEHKSGAVTYVQKAEDIMDFLIIIGAMSCKEDFEAIKLLREARNDIN
                     RANNAETANIAKTISASMKTINNIIKIMDTIGLESLPIELQQVAQLRVKHPDYSIQQV
                     ADALEFPITKSGVNHRLRKINKIADDL"
     gene            complement(37565..>37850)
                     /locus_tag="VHD02_000711"
     CDS             complement(37565..>37850)
                     /locus_tag="VHD02_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEQ5497341.1"
                     /translation="IDKKINRLNDLYINDLIDLPKLKKDIEELNHLKDDYNKAIKLNY
                     LDKKNEDSLGMLMDNLDIRKSSYDVQSRIVKQLIDRVEVTMDNIDIIFKF"
BASE COUNT        11429 a         6722 c         8060 g        11639 t
ORIGIN      
        1 ctatttaaaa aataaagaaa tagcaaaaaa cataaaatac ttgcattttc attgtaattt
       61 gctacaatag taaggtaaag ttagactgta tggcctaccg tctatctata aaatatattt
      121 tattggaggc ttttcctaaa atggcaaaag aaaaatacga tcgtagtaaa ccccacgtta
      181 acattggtac aatcggacac gttgaccatg gtaaaactac tttaacagct gcaatcacaa
      241 ctgtattggc acgtcgcttg ccttcatcag ttaaccaacc aaaagattac gcttctatcg
      301 atgctgctcc agaagaacgc gaacgcggaa tcactatcaa cactgcacac gttgagtacg
      361 aaactgcaac tcgtcactat gcgcacatcg acgctccagg acacgcggac tacgttaaaa
      421 acatgatcac tggtgccgct caaatggacg gagctatcct tgtagttgct tcaacagatg
      481 gaccaatgcc acaaacgcgt gagcacatcc ttctttcacg tcaggttggt gttaaacacc
      541 ttatcgtgtt catgaacaaa gttgaccttg ttgatgacga agagttgctt gaattagttg
      601 agatggaaat tcgtgacctt ctttcagaat acgatttccc aggtgatgac cttcccgtta
      661 tccaaggttc agctcttaaa gctcttgaag gcgacactaa atttgaagac atcatcatgg
      721 aattgatgga tactgttgat tcatacattc cagaaccaga acgcgacact gacaaaccat
      781 tgcttcttcc agtcgaagac gtattctcaa ttacaggtcg tggtacagtt gcttcaggac
      841 gtatcgaccg tggtactgtt cgtgtcaacg acgaaatcga aatcgttggt atcaaagaag
      901 aaactaaaaa agctgttgtt actggtgttg aaatgttccg taaacaactt gacgaaggtc
      961 ttgcaggaga caacgtaggt atccttcttc gtggtgttca acgtgacgaa atcgaacgtg
     1021 gtcaagttat tgctaaacca ggttcaatca acccacacac taaattcaaa ggtgaagtat
     1081 atatcctttc taaagacgaa ggtggacgtc acactccatt cttcaacaac taccgtccac
     1141 aattctactt ccgtacaact gacgtaacag gttcaatcga acttccagca ggtacagaaa
     1201 tggttatgcc tggtgataac gtgacaatca acgttgagtt gatccaccca atcgccgtag
     1261 aacaaggtac tactttctca attcgtgaag gtggacgtac tgttggttca ggtatcgttt
     1321 cagaaatcga agcttaattt taattaagtt cccagaatag caatttaagt cagacaactt
     1381 aagtttgaag aagtccccaa ttcgggggct ttttgctatc ttaaactcca taaaatactg
     1441 ataaataaga atgaaaagct ttcagaaaaa gagtttttat ttgtgaaaat attcctaaaa
     1501 tataagcaat tccttttaat cgtcggtcat ttgtgctaaa atagaagata taaataaaaa
     1561 aagaagaggt atgtgatatg tcacgtaaac caattattgc tggtaactgg aaaatgaaca
     1621 aaaaccctca agaagcaaaa gcattcgttg aggctgtagc aagcaaatta ccttcaactg
     1681 accttgttga tgttgccgta gcagccccag ctgttgattt ggtaaccact attgaagctg
     1741 ctaaagattc agttcttaaa gttgctgcac aaaactgcta ctttgaaaac acaggggcat
     1801 tcactggtga aacatcacca aaagtgcttg ctgaaatggg agctgactat gttgtgattg
     1861 gtcactcaga acgccgtgat tacttccatg aaacagatga agacattaat aaaaaagcca
     1921 aagcaatctt tgctaacggt ttaactccaa ttgtttgttg tggtgagtct cttgaaactt
     1981 atgaagctgg taaagccgtt gagtttgtag gtgcgcaagt ttcagcagca cttgcaggtc
     2041 tttcagcaga gcaagtagct tcacttgttt tagcttatga gccaatctgg gctatcggta
     2101 ctggtaaatc agctactcaa gatgatgctc aaaacatgtg taaagctgtt cgtgatgtgg
     2161 tagcagcaga ctttgggcaa gaagttgctg ataaagttcg cgttcaatac ggtggttctg
     2221 taaaacctga aaacgttaaa gattacatgg cttgtccaga tgttgacggt gcccttgtag
     2281 gtggtgcatc acttgaagct gatagcttcc ttgctttgct tgatttcctt aactaagtca
     2341 tctaaaaaag aaaaggttaa atccttttct tttttagatg acttaactct tactataaga
     2401 gaataaaggt gattctacag gcaaatgcat tagcctaata gcggtttaat agcttttttg
     2461 ccaatctaat gaaatgatat ttgattggaa atggataaca tctaaatgtc cctggttttt
     2521 gcaaaataaa gccattgaaa ttctgtttaa agcgcaagac accgtctgaa ccatcaaact
     2581 ttccagtaat accaagaaaa ttatattgtt tgatgccctt gtgaattgcc ttgagcatag
     2641 catgctcttg taatagagct gggctataaa atttattaaa tttggggtaa gatcctgaaa
     2701 agaggtagac ggcctcttgt tcggtataga taaaaagact tcccgcaagg aacacatctt
     2761 tagttccata ctcttcaaga aaatggagag cttcagaaat tcttacctga aaggtttcga
     2821 attggctgga tagttctttt aatctatttt gctttttttc agaatgagga tttttgccaa
     2881 ggtctccttt aactttgttg atgctggttg ctagttgtag ttgtctttgt ttaaggttat
     2941 taaggtaatc ttcaaaatta agagtcgcaa ccataaattc acaagagtct ccaaaactat
     3001 cataaaagtc ctgatagtaa gataatgatt tgtcaaggta atctctacga tcagaggttg
     3061 cttcagtgat ttctttgaaa tggtgtaact catctcgttt cagttgacgt aatttaatac
     3121 caaaagtatt agcttttttt attaaagctt ttcctttttt gctgaaggaa tgggttaaac
     3181 gtgaggaatc aattccttct aactttttaa cgtaatgcca aacaggttca ccctcaggat
     3241 agcctgtttt taaaccgtca tgtttatatc ctaacgccgt taaaaggcta ataaggtcag
     3301 tgtttggatg agaaataggg atgccatcag tatcaaatga ctgataattg tcgtaaggtt
     3361 ttaaaatgag ttcgataacg tctttttgtt tggcatagtc tttaagttga gtgtaaaaat
     3421 gttctaattc ttcgggatga ttggtattag ggcctgcatt gagttccata cgccaacctc
     3481 cagccacttt ttggctaaag accatagcag caacctttag ttcaccatct ttttcaagtc
     3541 caagaaattg tggttttgcc ccttttttag ccatcaagct ggccatttct gaggtttgga
     3601 taaaggaatg atggacgaga ctgtggcagt aatggtcaaa ttgttcttgt gaaatttcaa
     3661 ttagtgccat tagttgtgct ccttaatttt tttcttaagt tataggtcaa gatggctaag
     3721 cttgaaacaa ggccgacagg aatgttgaat tcacctgcaa attcttcgat ggtggggttg
     3781 agtctagctt tgaaatgata gagtccacca tcttgttgat tttccacacc tcctaaatta
     3841 tgccagcggc agcctctttt aaaagcttct ttagccgttt tataccaggt taaaagagga
     3901 gcttgataat tgcgataatc atcgtccatt cccgcataaa gattttcaga agtctcgcca
     3961 taaatcaagg ttagggtggc ggctagtggg atgcgggttt ggcctgtagc gagctgctcg
     4021 cttaaaatgg ttaaatcttt ttgtagccgt gcaatggttt tttgattttc tgctactttg
     4081 ctaggtttac tcttatctgt taatcgtgct tgttctgcta aggctttgtc caactgttgg
     4141 atcaagagtt ttttttgttc aggtaagtct aaactagcca tagtaatgta ggactgacca
     4201 gcataaatat ctagtaattt ttgatagtag gatttgcctc gaaggataat gccttttcga
     4261 ttctcggttt ttttcatcaa ggctgaaaag tcgtctagca attcactgcc accaatcgtg
     4321 acattaacac ctttattggt agcggtacga atggattgct tggctttttt aggcaggctg
     4381 tcaaagtcaa aatccttggc gtagatgttg gcttgtattc ttggttgaat agtgtcttcg
     4441 agttcttttg ttctgccaga ccattcgaca ccaagttttt ttagaaaagc aataatagag
     4501 agtgtcacat cattttcttt gctctctttt ccttctaaag tttgtttgat gactaaactt
     4561 ggatcaattt tgacaaaaag cgctcgtttg ctttttccga cagttttgag cgttttaata
     4621 acgaaatcaa ggagttcaaa attggcgtag tccatgattg gcccacgtgg gatataaatc
     4681 atagtgaaac caagaggaag ttttctgatt aaacaagcag cagcagcaac ttgtacccca
     4741 ttttcataaa agctgatgcg ttcgtgtttc cagttatttt tgacttttcc ccactttgaa
     4801 ctttgaagta agccagcttg tggctgtgct aagacaaact gatcatgttc ttcaggagat
     4861 attccgattt tatatgtata cattagttta atacccactt gcgaatagca tgtgccacac
     4921 cactgtcatt gttggatttg gtgatgtatt tggctatttt tttcaattca ggaaccccat
     4981 tttccatgac aacagggttg gcaacgactt ctaacatagc acgatcattt tcagcatccc
     5041 cgatggccat ggtttgtgac atatctagcc cgagtttttt agctaagtgt ttgatggcat
     5101 tacccttgct gacggttttt ggcatgaact ctaaataaaa aggggttgac ttgacgatgg
     5161 tgtacttatc aaagaaatgt tgaggaattt gcttgatggc cgcgtccaaa aggtcaggtt
     5221 catcaatcat catcattttg atgatttctt tattggtcat ttcttcggga gtgcgataga
     5281 aaataggcat gtttaccaaa gtcgattcat gaactgtgta ttttccaata ttccgattgg
     5341 cagtgtaaat accttctttg gtaatggcat gcatgtgaat tcctaatttt cggctcaaaa
     5401 attccgtttc cagataatcg tcgtaagtca tcaattcttt aacaatttct tcgccggtct
     5461 cggcatcttg caccaagcca ccattaaaag tgataacgtg gtttcctttg tggttgagtt
     5521 ctaattgttc gagtaatgaa atcactccgg caataggtct gccagtagca atcaccacat
     5581 gaaccccttg ggctttagcc tcttggacag cttgaaaaac atcatctgta attcgacggt
     5641 catcagtgag taatgtgcca tcaatatcga cagcaaccag tttgatagac atggcatcct
     5701 ccttgttttt ttgtggttta tcttaaaaat gttctccctc ctatcttact aaaaataggt
     5761 gcttttgtca tgggatggga aaggaaactt aaggaaaaac gagagaaaac gttttagttt
     5821 agctcaatcg cctctgtagt aactaaaaag attgaagaaa gtactctttc ttcaatctta
     5881 gaatggagac aaacccagtt tgatgctcaa aaagcatgaa ctgctctgct gatttgacaa
     5941 ctgatttctt atttctgcgt gtgacgtgag ccaaaacagc tgctttttgc tgtggcaaaa
     6001 gtggtgtcaa tactggcagt gacgttacgc ggaatttttg agaccctagg ctcaaaaata
     6061 aagcatagaa ttccaaagga agtcgctgcc ataagcatca cctaagaaaa gtatcaaaaa
     6121 agactttttc tgcaatctta aagggcttta ctgactaaag tgaaaatgcc cattggaaat
     6181 ataactcata aaagtttcct tgctaggagc aaagaggtca tcgagctcta acatttcctt
     6241 tggaaaataa aagcgacggt ctccgtaggt tgtccctgtt aatgctttga caataggaga
     6301 taactgagat aattcagcaa ggctcccgtc tttttgtatc atttcaatct gagttcttgg
     6361 attttcgagc tctggtcgat aaatatcata aggcaggtcg aagttgatat ggataccggt
     6421 gtaataatct ggatcaaagc caactgattc gaccaattgg cgcaagcgtt ctaactctcc
     6481 ttgggaatct tggtcaaagg taacagactt cagaattttt cgattaataa agcgactggc
     6541 caaatctgat aagatatggt cttcgcttgc catccagact tggaagtaag tattcatgac
     6601 cccgtcatct agggctatat aatctgctaa attagccttc ttttcaaaga aggggatgag
     6661 tccaggagct gttttttgga aataagcctg ttgctcagga tataagtgtt gggctctttt
     6721 gaggagattt tgtaagatga gctcaacagc acggctggcg ggatgaaaat agacctgcat
     6781 gtacatttga aaacgactga caatatagtc ctcgacagca tgcataccac tatgttcaaa
     6841 gacaatgcca tcttcaacag gtcgaatcac acgtaggatg cgcattaaat caaactgacc
     6901 gtaattggca gcagaaaaat aagaatctct taaaaggtaa tccatacggt cacagtcaat
     6961 ctggctggaa atcagttgca caacttgctt gttaggataa gtatgattaa tcacgctggc
     7021 aactttgtct ggaaaatcag gagcatgacg caccaaaatg gcattgattt ctgtctcagg
     7081 attggtaatt atctcctgag taaaggcctc gtgatccgta tggaaaaggg cctcaaaggt
     7141 atgagaatag gctccatgac caatgtcatg taagagggca gccgtcatag tgaccagact
     7201 ttcatcttta ttccagatat cagcgtattt ctcttcaaaa atagctgtca cccgacgggc
     7261 aatttcgtaa acacctaggc agtgagaaaa acggctatgt tctgcgccgt ggaaggtgaa
     7321 agctgttgta gggacttgtt tgatgcgtcg taagcgttgg aattctttgg tattgataag
     7381 gtcataaatt aatggattat caatgtggat atagttgtga acagggtcac gaaatacttt
     7441 ttcattcata atttttatta tatcaaaaat tgataaaata aggagaaatt gatagaataa
     7501 acagacgagc ttagaaatag aaagaagagt tatgaaatta cagttaacca accatattcg
     7561 ctttggagat gagacagaga ttattcaaga aattcatgat tgtgagtggc gagaaaaagg
     7621 gggctatcag tacctcatct atcaaaacac tgacaaggaa aaggttgtca tcaagtataa
     7681 tgagacagaa ctgaccatga gccgtttttc caaccctcag tcaatcatga aattttttgc
     7741 gggcaagaag gtcctgattg ctctacctac accgatgggg gttcaacaat ttttgacaga
     7801 taccagtcat taccacttag attgttcttg tcaaaaattg gaccttcatt atcatttgct
     7861 tcaagctcaa acagaaatgc tatttgcaag ctatcatctt gagctgagtt gggattaact
     7921 aaaaagatag cagtaaatca tctgattagg gattattctt gttttttagt caggttttta
     7981 ctataatggt tattatatca agggagtagc agacggctag ccgtgattgt atcgtcaata
     8041 cggaatttcg gtttccggta caatcttaaa cagcgagact tgtttaagaa aataaacagg
     8101 tctttttatt tgaacaacta aaataaaaag accaaggagg tactaatttg tctaaagaac
     8161 aaagacatga agcattttac acgcaaagtg aagagaccgt actggctcaa ttagagacca
     8221 gtcgcgaagg tcttacgtct gcccaagcaa aagagcgctt agcagagtat ggtcgtaatg
     8281 agcttgatga aggcgaaaaa cgaagtttat ttatgaaatt ccttgatcaa tttaaggatt
     8341 tgatgattat tattttgatt gtggcagccc ttctttcagt tcttacagag gggatggaag
     8401 gtttgacaga tgccattatt atcttggctg ttgttatcct aaatgctgct tttggtgttt
     8461 accaagaagg acaagctgaa gctgctattg aagccttgaa atcaatgtca agtccactgg
     8521 ctcgtattcg ccgtgatggg catgtgactg agatagactc taaagaattg gtccctggag
     8581 atattgtctt gctagaagcg ggagatgttg tccctgctga tctgcgtctt ttggaagcta
     8641 actccttaaa aatcgaagaa gcagcgctta ctggagaatc tgtaccggtt gaaaaagact
     8701 tgtcaacggc tgtttcagaa gatgccggta ttggtgatcg cgtcaatatg ggatatcaaa
     8761 actcaaatgt gacatatggt cgtgggattg gggttatcac taacactggc atgtacaccg
     8821 aggttgggca tattgctggt atgctttcta atgctgatga gacagataca ccattgaagc
     8881 aaaaccttga caacctttct aagattttga cttatgcgat ccttgtgatt gcagcggtga
     8941 cttttgctgt tggagtcttc ttgcgtggtc aacacccgct tgaaggcttg atgacttcag
     9001 tagcgcttgc cgttgcagct attccagagg gacttcctgc gattgttact gttgtgcttt
     9061 ctcttggaac tcaagtactt gctaaacgta acgccattat ccgtaaatta ccagctgttg
     9121 aaacgctagg atcaactgaa atcattgctt ctgataaaac gggtaccttg acaatgaatc
     9181 aaatgaccgt tgaaaaagtt tataccaacg ggacactcca aagttcatca gctgatattg
     9241 cttttgacaa tacgacactt cgtgtaatga actttgctaa tgatactaaa gttgatcctt
     9301 cagggaagtt aattggtgac ccaacagaaa cagctttggt acagtttggg ttagaccaca
     9361 attttgacgt acgtgaggct atggtggcag aacctcgtgt ggcagaatta ccatttgatt
     9421 ctgaccgtaa gttgatgtca accattcata aacaagctga tggcaaatat ttcatcgctg
     9481 ttaaaggtgc gccagaccaa ttgttgaagc gtgtcacaca gattgaagaa aatggacaaa
     9541 ttcgtcctat tacggatgct gataaaaaaa ccattcttga taccaataag agtcttgcca
     9601 aacaagctct tcgtgtcttg atgatggctt acaaatattc tgatgctctt ccaacactgg
     9661 aaacagaaat tgttgaggct aatcttgtct tctctggttt ggtagggatg attgaccctg
     9721 aacgtcctga agcagcacag gctgttaaag tagctaagga agctggcatt cgtccaatca
     9781 tgattactgg tgaccaccaa gatacagcaa aagctattgc gaaacgtctt ggtattatcg
     9841 aagaagatgg cgttgatcat gtctttacag gtgctgaact caacgaatta tcagatgaag
     9901 agttccaaaa agtctttaaa caatactccg tgtatgcccg tgtatctcct gagcataaag
     9961 ttcgtatcgt taaggcttgg caaaatgaag gcaaagttgt tggcatgact ggcgatgggg
    10021 ttaacgatgc accatctctt aaaacggcag atattggtat tggtatggga attacaggta
    10081 cagaggtttc taagggagct tctgacatgg ttcttgccga tgataacttt gcaaccatta
    10141 tcgttgcggt tgaagaagga cgtaaggtct tctcaaatat tcaaaaaaca attcaatact
    10201 tactttctgc taacatggca gaagtcttta ccatcttcct tgcaaccttg tttggttggg
    10261 atgtgcttca accagttcac ttgctttgga ttaacttggt aactgatacc ctaccagcta
    10321 ttgctcttgg tgttgaacct gctgagcctg gtgtgatgaa acacaaacct cgtggacgta
    10381 agtcaagctt ctttgatggt ggggtgaagg aagccattct ttaccaaggt gccttccaaa
    10441 ccattttggt gcttggggtt tatggttttg ccttgatgtt cccagaacac acaagctacc
    10501 atgatgtcca tgcagatgct ttgaccatgg cttatgtgac cctcggtttg attcagttgg
    10561 tacatgccta caacgtgaaa tctgtttacc aatccatctt tactgttggg ttgttcaaaa
    10621 acaaattgtt caactattct attccagtgg cctttgtagc attgatggca acagttgttg
    10681 ttcctggatt taatcaattc ttccatgtga cacacttgac gatcacacaa tggttagtgg
    10741 ttattatcgg aagtctcttg atggttgttc ttgttgaatt ggtgaaagct gttcaacgat
    10801 cacttggtca agatgaaaaa gcaatttaat agacgctaaa aggtttgaga caattgtctc
    10861 aaaccttttt ttataatcgt tacaacgata aggactattg gtatgcctga cattaaaaag
    10921 ttgattcttg ccaattgact tgacagtcaa aaatttgttg tttggcttct tgactgtgac
    10981 cttcaatatc atcgattaaa atcttagtag cctgttcgcc ttcttcatag gcgggttgga
    11041 taatagttgt tagtgttggt gaagaaaatt ttgtccactc aatgttatca aatcctacta
    11101 gtccaatctc tggtatatta aatttaagag acttcatagc tgtaaatact tttggaagag
    11161 cccaacaatt ggggacaaaa accaaactct ttttagtcag tgatccttgc aaaaactgag
    11221 caattgcctc tgaagaagtc tgattttcat caataatcat ttcttgatga gtaagatgat
    11281 tagcttctaa cacatcaatg aagcctgaag cgcgttcaat acgagtgcta agtaaattgg
    11341 ggttacccgt gatcatgata aaatgttcat aacctttatc gatacattgt tgaatggtat
    11401 cataaacagc atcataatta ttagttttga cccagttggt acgatgctca taaagttgac
    11461 tatcaaaaaa gacaaccttc ttttttttaa tatcgataat gcgagaatac ttcctgaaat
    11521 tagaggtagg ttgaatgata aagccatcca ctccgaggtt cagcatcttt tcaatgagtt
    11581 catcttcgcg agaagggtcg tagttgctgt ttccgatgat aatttggtaa ccaaattcct
    11641 gggctttgct ttcaattccc ttaacaattt gattggaaaa actattggta atatcaccaa
    11701 taaccacgcc aatgagttta gtagatttgg cgtttagact tctggcagca atgcttggtt
    11761 tatagttggt agcttcaatc gtgcttttaa tgcgattttt agtttcttct gacatcttgt
    11821 caaatttacc gttcaagtaa aaggaaactg tcgttttgga agttttagcc aattcagcaa
    11881 tatctttgat ggttactttg cgctgcattg catacactcc tttaatttag taatggttag
    11941 tcatattata acataaggac tcgtaaaaga tttagtgttt caatcgaata atctcattcg
    12001 tgatatggtt gataacaacc acctttcctc tgattttatg accatttagg agaactagtt
    12061 tttctccttt aattaaatcc tcttggagaa cacagatact gtagtcaaac tgctttcctt
    12121 ttaaatgcca ggaaagggcg gtctcaattg ggttgcgttt gcctgtttga agtggtgtta
    12181 agggagtaac tttggtatcg tcagggacta atagggtact ggtgcaaccc ttgtctttaa
    12241 agggttttcg tttgatgagt ttgtgactag tcaggtattg gttgtatttt tttgacaaaa
    12301 tcgtttcttc gatagtgaaa gggcagtcac tgattagtct gaggtcacta aggtgatctg
    12361 tctttacttc taagtgatta tcaagaataa attgactgac aagacagtgg ttgccttgag
    12421 cctgtatcgt atcaatgata agaaaaattc ctggtggtaa gatgagaatt tgtcggtcat
    12481 gttggtagtt gttcctatca gagaattgcg tctggtagct accatcaata aagtaaatgt
    12541 gtccattttg tcgaaggtga caataattgg atttggggta agaaaggtaa gcccaggagc
    12601 tatcaatctg ctcaggtaat tgttcttcta agaaggcggt agaatgatgg ctagcgcttt
    12661 ttagggcata gcgtaatggt tcttctttgt aagtgtatcg cccagcatca cagaaaagtg
    12721 gttgtccttt ataatagaga cagatactat tttggtcgct atgggtatgg gaactaccga
    12781 tgggcccatt tttgaaaaaa gataataatt ttcttgattg atggtaatgt gcccggaatg
    12841 ttcaaagtga tgcgcaaaag ttggtaaggt ttggacgggt aactgttcaa aaatgtggac
    12901 acctttttct cctagaagga gaagactatc gatatcaagt gtgggaaaag cagcagcttt
    12961 taagtgtggt tcttcaagga tagtggctgc tagtgttaga atatctctgg tatcggtaaa
    13021 gtcactgtct cctaagggga tctgtttgtg gtcagggcca gtcattttga ggagataatc
    13081 actcatggca agcaaagtag gtctaagctg tggaagataa tcagggacta gagcagttaa
    13141 ttccagaagg gctttgagta cttccacatg atacatggtt gattgttcaa attgactgcc
    13201 gtcttcgaga atttgtagtg cgatttgttg tgtcaattct tttctagcga aagctgttgc
    13261 tgcagcaata tcaaggttgg aaccaaagta ggcatcagct aataaaatcg cagtggtttg
    13321 caagattccc caattgctga gactgtattt atcaaggtag ttggcatgga gaaagtgaag
    13381 ttgtttttcc agggaagcta ggatgagcct ttcctcttgc ttggttaagg cgtcaaaaag
    13441 attaaggtaa atcaaacatt tgacccaagc aaaacaacgg atgccagtat ctaaggttct
    13501 agtagctaac cccttagggt ctaaaggaat agctgattca atccaattga ggatgaaact
    13561 tttggctttt aggagatacc gttcatctct ttcaacaagg tagacgagga ttaatgtttg
    13621 gagataggtc tgtcgattta acataaaatt ccactcagga tcatctgtga cagcctcttg
    13681 ccaagtaatg ggatctaagt ggtaagggat gtggcagggc tccatatccc aattatcttc
    13741 aaataaaaag gtattggtta agagtaggtc agctattctt ttttgatcag catagctgtc
    13801 agtctgatag tctagcaggt aattcctgca aaagtctgga ttgactgttt ctttaaagcg
    13861 tgcgaaagca agagacatag gacgtttcct ttccaaaaga aaagagacct catcatgtgc
    13921 ttaggaacta tttccctatg ctcataacag gtctctgtga accattaagc taaaatacct
    13981 aaccagctac cgataatacc gataagaaca gtgaggagaa ccaatttgta ggttgtccat
    14041 tttttgtttt taataaggta atacatgagt aaggtaaaga gagctggtaa aagtgcagga
    14101 gcaattttat caagcattcc ttgaatagtc acaaattttt gtgctgtatt ggccgcatcc
    14161 accttgccag cggcaaaagt aatgggaaca gttattttaa cagaagtagc agctaatcca
    14221 gcgataacgg taacaccgac aatatttgcc atgcgcgaca caaccgccat ttgttcactt
    14281 aatttatcaa taaagcttgt gccgaccttg tagccataga gaccagaacc gattttaata
    14341 gcagttagga taatattcat ggctaggaag aagaaaattg gacctaaaac taaaccatca
    14401 ctagctaagg aagctgcaat cgttgaaaag agtggcgcca agcaaaattg tgataaggag
    14461 tcaccgatac cagctaaagg ccccataagt gccattttga tgtttcgggt ttcttcttca
    14521 ggacgatcat tttctaacat gaccaagtgc agactggtga tgaaaggaag gaagtgaggg
    14581 ttagtattgt aaaactcaca gttatcttct aaagcttgat ataagccctt tttatcactg
    14641 ccaaaatgtt ttttcaaggc aggatagatg acgttggcgt atccgatacc ttggtaatta
    14701 ctgtaattaa acccattttg taggaagaaa gctctcagag ctgttttgag gtaatctttc
    14761 ttggttaatt tattagatcc agtcatcttg ttggcctcct tggacagttg ttgcatcgac
    14821 ttgtttaggt ttgttgtaaa attcattcaa ggcgaaaatg ataccaataa tagcgatacc
    14881 aatggttgga atttggaggt aggctgcaca aacataacca atcagtacaa aaggaatcaa
    14941 ttctttcttg gccataacag ataagatcat ggcaaatccg atagcaggta acattttacc
    15001 agcgacagtc aatccattga gcaagacagg tgggatataa tcaaccaagt gaaggagagt
    15061 atccattgac aaggcaccta ataaaccaag gcctaatccg ataaaagcga aagcccagat
    15121 agtgccgttg gcagcgaatt tgaagcctct aatattgcct ttttgcaatt gctttttagc
    15181 ggtttcggga gcgccagcaa aagctgtata ggcgaatgtc tgaagaaatt ggatgacaac
    15241 tgcgattggt gttgataagg ctagggccgc ttctggggta actttaccag cactggtaat
    15301 agccatcaag gtaccaaaga taccagggcc gataggatta ggaggaacgg taccaccggc
    15361 acccactcca aatcccatat aagctaattc ggagatagct cccatgctaa gtgcagttgg
    15421 caagtcacct aagataatcc ctacaccaaa agacagaata atacaacggt tggtgtagat
    15481 gccaagtaac attccgctga aacaaaaggc tgtccaaaga ccaattaaaa gtgcctgtaa
    15541 caaattgata tccatatcaa tctcctttat ttattaaata taatctaaga tgtcaacgtc
    15601 agaggcgcta ttgccagctg gagttgtttt ggtgttaaaa accacgtggt ggtcatgggc
    15661 taagcaacga atagcagact tgtctgtttc tcccagcgaa ataaactggg taatcgcaac
    15721 cttatcatca gttttatgaa tattaccgat attgatttct gtgatgggaa cacccccttc
    15781 aactaggcgt ttggcatctt gcaaatcttt gaccacgatg aagatatttt gagcaggact
    15841 tgctttatga atgatgtcaa tcactttttg aatggagaaa aaacgaatgg caatactgga
    15901 tggaataact gttttcatga gggattgttg aatcttatct tcagaaaccg catcgttagc
    15961 gacgataacg gtgttgcagt tgaggaattt gacccataat tgcccctgtc cgtgaatcaa
    16021 gcgctcatca actcgcgtca tgataatgtt tggttgtgtc ataaaatctc ctttttaaat
    16081 gtgattatcg gtgtttgtta ccagtaaggg ttccagtctt tgtagaaacg aagcagagct
    16141 tcaaggtagt aatagtctcc ccagatattt ccttcatcca ccccttttcc agagtgccaa
    16201 gaatagactc cgtgaagcag tagaggagca ccaggcttga gatttttgtt agcataatcc
    16261 ttaatcaagg ctcttagcat gctgtgcatg gcagcttcat atgttttttt atcaggatca
    16321 tgatctggta aggtttttaa catttcatga ataccacaca cagcaatggc tgttgctgaa
    16381 ctatcacgag actgtccact tccatctcta aaaattaaat cccagtagga tacctggtct
    16441 ttaggaagac ggttcaagaa ataatgggtc atgcccttga agagttgaat gagttcaggt
    16501 tcttttagga aacgataggt caaaggaatg ccataaatgc cccaagcttg ccctctggcc
    16561 caagccgaat catcgctata accttgtcgg gtgacccctt taacaggatc ccctgtttct
    16621 ggatcaaagt agaaggtgtg gtaagcagag gcgtcatctc ggatgacatg attggctgag
    16681 gcgtagaaat ggttgatcgc catgtcacgg taacggttgt ctccagtttc ttggctagca
    16741 aaaaagagta attggatgtt taggagacaa tcaatgatta aacggtaatc ttcttttttc
    16801 cccagttctc cccaggcctg aataaagccg cctttgtctt gatagcgttg gactaatttg
    16861 tcggctgctt tcaaagcagc ttctctagcc tcaggtgttt ttaaaagttt ccactcagcc
    16921 atgcaagaag gggtgtaaag aaagcccaaa tcatggtgat ctagctcaat gtctcttgtt
    16981 actcgatcta ggaaagaaag gtcatttgct tgagccagag ctttaatggc atcatcccca
    17041 ctgtattcgt aagctaacca aaggcaacct gtccaaaagg cattggtcca ttccgtgttg
    17101 tccataatgg cgtattggtt atctttggta gctggcgttg gaaagtcttc tttaaaatag
    17161 tccatattaa gccgaacttg tttgagggct aagtcaaggg cctgagtaat gtcttcttgg
    17221 cttagaaagt cttctttggt aaatcgttca ggctgcttga taggttcaag agcaatggtt
    17281 tttaaagatc gtgtcattaa atgcctccta actcatcgtc tgtcgcaccg tcttcttgct
    17341 gagaaagagt tttccagtca acaatgcctt ctttagaaca tgttataagt cctgatacta
    17401 agtcgtcaaa agaagctgct gtttgtcttg caaaactagc ttcaatcagc atggctaagt
    17461 taaggccaga aagaacatta cacgtcgtgt ttggaagaga ttccattaag gttgcagcga
    17521 ctttaaaagg ggtacctcct agaaggtcac aaagaactaa ggtttcctct tctgggatta
    17581 aagcacgaga taattggtcc tgcacatcag cggcggtcat ctcagcagta aaatcaattg
    17641 cagttacctt ctcttgtggt cctgcaatta actctaggga tgagacaatc ccacttgcaa
    17701 agtgtccatg tcccataaca ataatagcaa tcatgataaa actccttttg gtttaccggt
    17761 ttactcaaaa acattgtagc gctttcaaaa ttctttgtca atactttttt gagatttatt
    17821 tttagagaaa atagagatta atctccataa catgtcaaaa gggatcaatc atagttgttt
    17881 aatgaagaac actatgtaaa agaaatgcca ttaaggaata aaacctattt ttaaaagcta
    17941 gctagtttct cttttttatt tttgaaccgt tttcataaac atcttgacaa aactagttta
    18001 ctttgttata gtcttagtaa acaggtttac ttaatttaat aacaaaaagg agaatcctat
    18061 ggaaaatatg ttttcgttac aaggtaagat tgccttgatt accggagcat cctacggtat
    18121 tggttttgaa attgccaaag cctatgctca agcgggtgca actattgtgt ttaatgacat
    18181 caaacaagag ttagttgata aggggttggc tgcctatcga gagttaggga tagaagcgca
    18241 tggctatgtt tgtgacgtga ctgatgaagc tggtatccaa cagatggtta gtcaaattga
    18301 agatgaagta ggtgttattg atattctggt caacaatgca ggtattatta gacgtacgcc
    18361 gatgcttgaa atggccgcag aagattttcg tcaagtcatt gatattgatt taaatgcacc
    18421 ttttattgtg tcaaaagtag ttttgccatc gatgattgca aaaggacatg gtaaaattat
    18481 aaatatctgt tctatgatga gtgaattggg ccgtgaaact gttagtgcct atgcggcggc
    18541 taaaggtggc ttgaaaatgt taaccaaaaa tatagcctct gagtttggag aagccaacat
    18601 tcaatgtaat gggattggtc ctggctacat tgcaacacca caaacagctc cgttgagaga
    18661 gcgacaagca gatggtagtc gtcatccttt tgatcaattt atcattgcta aaacaccagc
    18721 agcacgttgg gggacaacag aagacttagc tggtccagct gtttttctag ctagcgatgc
    18781 cagtaatttt gtgaacggcc atattcttta tgtcgacggt ggcattttag cttatattgg
    18841 aaaacaacct taatcacaca ctagaaagag attttatatg aaaattgcat taattaatga
    18901 aaatagccaa gcggctaaaa atggtattat ttacgatgcc cttacctcag tgacagataa
    18961 acacggctac caagttttta actatggcat gtatggcaca gaaggtgaaa gccagttgac
    19021 ctatgttcaa aatggactct tagcttctat tttattgacc actaaggcgg ccgattttgt
    19081 ggtgactggt tgtgggacag gtgttggagc tatgttggcc ttgaacagct tcccaggagt
    19141 tacctgtggt tttgcgagtg agccaacaga agcatatctc ttttctcaaa tcaatggtgg
    19201 taatgccttg tccattcctt ttgccaaagg ttttggttgg ggagcagagt tgaatttgac
    19261 cttaatattt gaacgcctct ttgcagaacc aatgggtggt ggttacccta aagagcgagc
    19321 tattcctgaa cagcggaatg cacgtatttt aagtgatttg aaaaaaatta cttacagaga
    19381 tttgttggca attgtcaaag acatcgacca agatttcctc aaagaaacga tttctggcgc
    19441 tcattttaaa gagtacttct ttgccaatgc ggagccgtca gaattagtcg cttatttgaa
    19501 atcagtactt gaacagtaat tccattttga cgaggaaagg aggtcacatg tctaagttat
    19561 tattggttgg tgaaccgctg attcgcgtta gccccaatca gtttcagcct ttgaccaatg
    19621 cctgcgacgc tcagcttttt ttcggcggtt cagaagttaa tattgcaaga actctaggcg
    19681 gttttggctt agaagcaagg ctatttacgg ctctaccaga taatcctgtt ggccacgctt
    19741 ttcatcagtt tttgaagcaa agcggtgtgg atatgacctt gacggcttgg caaggtcatc
    19801 gcgttgggct atattacctt gaaaatggct ttggctgtcg ggctagtcag gtttattatg
    19861 accgctgtgg ctctagcttt tcggctttag ataaggacag ccttgattta gctgccattt
    19921 ttgaaggcat tcgtcacttt cattttagtg gaatcagttt ggcgttgggt aaaaaaactc
    19981 aggatttgat tgaaatactt gcgagagaag ctaagaaaag ggatatttgt atttcctttg
    20041 accttaactt tagatctagt atgattgccg tagctgatgc taaacgactc ttttctcact
    20101 ttgctcaata cgcggatatt atttttggca tggaaccttt gctgttagac agtgacgatt
    20161 ttgacatgtt tgataggaat aaggcagata ctacgacgat tagagaacgt ctggcaggac
    20221 tttatcagcg ctatcagtta caagctattt accatacgga gcgttcaaat gatgcccaag
    20281 gcagtaatcg tttcaaggct tatgcctatg atgggcagtt ttacgaatcc tgtgaagtga
    20341 cgaccccagt cttgcaacgg gttggaagtg gagatgcttt tgtggcgggt ttactttatc
    20401 agttgttaga aggtaacgaa aagcagcgca accttgattt tgctgtggca acagctagcc
    20461 taaaatgtac agtagctgaa gatcagctct atcacagcgt ccaacaagta gaagcagtct
    20521 tacataatca acgagatgtc cagaggtgat gatatgggaa aaatagaaat cttaaccaaa
    20581 cttaaggcta atcggcttgt cctggttgta cgaggagagt ctagtgagga agctttagct
    20641 tgcagcctgg ccagtattga aggtggcatt aagacaattg aagtcaccta tactaacccc
    20701 tttgctagtg aggttatcgg tcaattagct gaacgcttca aagaagatcc tgaggtattg
    20761 ataggtgctg ggacagtttt ggatgatgtc actgcccgcc aagctatttt agcaggagca
    20821 cagtttattg ttggtcctaa tttcaatcga gcagtggctt tgatttgtca tcgttatagc
    20881 ataccttatt taccaggttg tatgacggtt aatgaagtgg ttacagcatt agaatcaggg
    20941 gttgatatgg tcaaaatttt tcctggttct actgtaggga tgtcctttat tcgggcaatc
    21001 aaatctcctt tgccacaagt agaggttatg gttacaggcg gagtttcttc agataatctc
    21061 aaagactggc tagctgctgg agtagatgtt ttaggcatag gaggcgagtt taatcagtta
    21121 gcgagccaaa aacaatataa cctaatcact aagaaagccg ctcattatat caaaagtttg
    21181 cgacaataat ctaaaaaacg tttttacctt ggctttagtg ctagtaaaaa cgctttttta
    21241 tcattcattc aattaatatg cttctgctta tgtttttcca gatagtatag atataagagc
    21301 agctaattta acaagatgag cctgtcgttt gatatatagg atataaactt tttatgatca
    21361 aacaaaagta ataccttttg aattcgttag tattgacctg atctaaagtg atatcactta
    21421 gaatgaacta gctgtgcagc ttttacagca acttatgcta aaatagaaaa aaaggagaaa
    21481 tgttgatgat taaaggaatt atttttgata tggacggtgt tttatttgat acagaacctt
    21541 tttatctgag gcgacgagaa gattttttta agacaaaggg aattcccatt gatcatttga
    21601 actctaaaga ttttattggg ggcaatctcc aagaattatg gaaagagttg ttaggtaaaa
    21661 atagggatga tgctatcgtt aaggcaatta caactgacta tgacgcttac aaacaagcgc
    21721 ataagcctcc ttatcaaaaa ctgttgatta cagaagtgaa ctcttgtctt gaacagttga
    21781 aaaaacaagg tattaaactg gctgtggcat caaactcgaa gcgtcaggat gttttgttgg
    21841 cgttggagac aacgcaaata aaagattatt ttgaaataat cctagcgcgt gaagatgttt
    21901 ctagaggcaa accttatcca gatatctata ataaagcagt acaaaaacta ggattacaaa
    21961 aaaaacaact gcttgtagta gaagacagcc aaaaaggcat tgctgccgcc aaagcagcca
    22021 atctgacagt ttttgccatt accgactacc gatatggcat tgatcagagt caagctgatc
    22081 acaagataga tcatttagga caactgtgtg taaaaatcgg ttgttttgga tcgtgaagta
    22141 acgcaactaa ctgatcattg atgatacttc aagacactta agaatacgtc taggactttc
    22201 tacagaccaa taacaagaat acgtttatgt gtttttactg ttttttttag ctacctattt
    22261 acaatatgac actgaaaaga gtactatgtt taagttatta tcaaaaaaac cgtcgcataa
    22321 gatcacctag aaaaggatta tgatttttgt gatttggatg cgattgttag tcgttgacca
    22381 tctttatgtg gcaggagata tgtttaaccg tggtcattat ttggatttgt ttttagattg
    22441 gctagtgagt aaatatagaa ggatcataat actgctttga caaaggcagt atctgagtcc
    22501 atgagttgca cggataatgt gactcggttt tggttagcta taataatatt tggaagtgtt
    22561 atgctgcttg tcttcgtagg tccattgatt atagtcagac ttactttaga gctgagctga
    22621 ctgtctagac aagacgtgat ggagatgtga tttctgacca agtagaggta accagaaaag
    22681 ggacttttac gatgtaattg aatttacttg ttgcattctt gtgcatgtta agagagaaaa
    22741 cccagataaa tcaggaagta aaatggttat gtttgattag aattgtcctt ctttttcctt
    22801 gaagtcttaa cttattttga ttatgatagt agtatgacta ttaaggcaga aattaaggca
    22861 ctcgccaaag aaattggtat ctctaaaatt ggttttacga cggcagataa ctttgattat
    22921 ttggaaaagt cattgcgtgc ttctgtggaa gaggggcgca attcaggatt tgaacataaa
    22981 gtcatagagg atcgtatctc ccctgaaagg cttttagaat cggctaaaac gattatttca
    23041 attggcgttg cttatcctca caaacttcct cagcaacctc aaaaaacacc ttataaacgt
    23101 ggcaaaatta cacctagttc gtgggggttt gattatcatt atgtggtcgg cgaaaagtta
    23161 gacagactat caaaaggcat tgaagagctg tgtcgagatt ttcctttgca acaaaaagca
    23221 atggtcgata ctggagcttt agttgatact gcagttgccc aaagagcggg tattggtttt
    23281 ataggaaaaa atggtctagt tatttctaaa gaatatggct cttatatgtt tttgggagaa
    23341 ttaattacca atttagagat cgagccagac aagcctgtcg attatgattg tggtgactgt
    23401 cgtcgttgta tagatgcctg tccaacttct tgtttaattg gtgatggctc aatgaatgcc
    23461 aagcgttgtc tatcatttca aacccaagat aagggaatga tggacataga gtttcgaaaa
    23521 aagattaaaa cagttatcta tggttgtgat atttgccaaa tttgctgtcc ttataacaaa
    23581 ggtataaata atccaccagc tacagagatt gatcctgagt tggctcaacc agaattaata
    23641 ccatttttga gtttatcaaa tggacaattt aaagaaaaat ttggtatgat tgctggaagt
    23701 tggcgaggta aaaatatttt acagcgtaat gccattattg cgctagcaaa tgctcatgat
    23761 aaaaccgcag tggtcaaatt aatagagatt attgataaaa ataataaccc tattcatact
    23821 gcaacagcta tatgggcatt gggagaaatt gtcaaaaaac caaacgatga gatactggcg
    23881 tttatgagtc atttaacctt aaaagatgaa gatagccaaa aagagttgga attaatccgt
    23941 cataaatggc aattttaagc cttttatggt atttttaaga accttatgat aaaataaaac
    24001 agattaacaa agattggaga aatctcatgg aagtggctga aattcgccaa aaaatagtag
    24061 aaaataaaga gaagttgact agcttcagga ggtctctttg acttagatcg tttggaagag
    24121 gagattgctc ttcttgaaaa tcacatgact gagccagact tttggaatga caatattgca
    24181 gcgcaaaaga catctcaaga actaaatgaa ttaaaaggta agtatgatac tttccataat
    24241 atgcaagaat tatctgacga aacagaactc ttacttgaga tgcttgacga agatgattct
    24301 ttgaaagaag aattagaaga aaatctaatg cagctagata agataatggg tgcttatgaa
    24361 atgacgcttc tcttatctga accctatgac cacaataatg ctatcttaga aatccatcct
    24421 ggttcaggag gaactgaagc gcaagattgg ggagatttac tcttacgaat gtatacccgt
    24481 tttggcaatg ctaatggttt caaagtagaa gtattggatt atcaagcagg ggatgaagct
    24541 ggtattaaat cggttaccct ctcttttgaa ggtcccaatg cttatggttt gttgaaatca
    24601 gaaatgggag ttcatcgcct cgttcgtata tcaccttttg attcggctaa acgtcgtcat
    24661 acctcatttg cttcagttga agtgatgcca gagttagatg atacgattga agtagaagta
    24721 cgtgatgatg acattaaaat ggatactttc cgttcaggag gagctggtgg tcagaatgtc
    24781 aataaagtat caacaggtgt tcgtttgacg catattccaa ctggaattgt ggtatcgtct
    24841 accgttgacc gtactcaata tggaaaccgc gatcgcgcta tgaagatgtt gcaggctaag
    24901 ctatatcagt tagaacaaga aaagaaagca caagaagtag atgctctcaa gggtgataaa
    24961 aaagaaatca cttggggaag tcagattcgt tcttatgttt tcacccctta tacgatggtg
    25021 aaggatcatc gtactaactt tgagcttgct caggtagata aggttatgga tggagaaatc
    25081 aatggcttta ttgatgccta ccttaagtgg cgcattgaag actaaaaata ataaaggaga
    25141 aacatggcac taatagaaat gtctggtgtt actaaaaaat accgccgctc aacgaccgcc
    25201 ctcagagacg tcaatgtttc tgttaaccaa ggtgagtttg tttatctcgt aggcccatca
    25261 ggtgctggta aatcaacttt tatcaaactc ctttaccgtg aagaacaact gacaactggt
    25321 aagctatatg tgggtgaatt caatttaact aaactaaaag cacgtgatgt tccgattctt
    25381 cgtcgtcaca tcggagtagt ttttcaagat tacaaactgc taccaagaaa aacagtcttt
    25441 gaaaatgtag cttacgccat ggaagttatt ggtgaaaagc gccgccacat taaaaaacgc
    25501 gtgccagagg tactcgattt agttggcttg aaacataaga tgcgttcttt tccaagtcag
    25561 ctatcaggag gggagcagca acgtgtggcc attgcgcgtg cgattgttaa caaccctaaa
    25621 ttattgattg cagatgaacc gacagggaat ttagatcctg agatttcatg ggaaattatg
    25681 cagttacttg aacgtattaa tgttcaggga acaactatct taatggcgac acacaacagt
    25741 catattgtga atacattccg tcatcgtgtt gttgctattg aagatggtcg catagtacgt
    25801 gatgaagaaa aaggagatta tggttacgat gattagatac tttttccgtc atatttggga
    25861 atccattaaa aatcttaaac gcaacttttg gatgacattt gcttcagtta gtatggtggc
    25921 tgttacattg actttagttg gtgtttttgc ggcgacattg ttgaatatac agcgtgttgc
    25981 ttcgggtgtt gaaaataatg tccatatcaa tacctactta caagttgatt caactgatgc
    26041 tgcaaaagtt attcaaaata ctgccggtga acctgttaat aacgataact accacagtgt
    26101 ttacgacaaa attgctcaga taaaaggtgt caaaaagatt acattttcta gcaaggatga
    26161 acaattgaaa aaattgcaag aaaccttagg tgatgtttgg aatatgtatg accaagacac
    26221 taatccgctc caagatatct atcttattga gacccagacg cctaaacaag ttaaggcgat
    26281 cactaaaaag atcagaacga ttgaaggcgt agaagccgca gattatggag ggattaactc
    26341 cgataaacta tttaagtttt caacattaat tcaaacttgg gggctgatcg gaacagcaat
    26401 gctattattt gtagctgtct ttctcatttc aaacactatt cgtatgacta ttatgtctcg
    26461 taaacgcgac attgaaatta tgcggttagt tggtgctaaa aattcttata ttcgagggcc
    26521 tttctttttt gaaggggctt gggttggctt gttaggagct gttttaccat ctcttctgat
    26581 ttattatgga tatgatcttg tttataagca ttttgcacaa gaattacaac gtaataatct
    26641 ctcgatgtat ccgcttgatc cttatgtata ttacctgatt ggtgctttgt ttgtcatagg
    26701 tattatgatt ggttcgcttg gttcagtgct ttctatgaga cgttatttaa aattctaaaa
    26761 aagcgctgct gacgaaacgt agcgaccctc aaaagttgga cctcttatgt tattccaagg
    26821 acttcgtttt gtattgcaca ggactggtcc ttttcatttt gttttgattc atttgtttat
    26881 ttgtaatagg caatatagtt taaaatagca gtttctaagg tttcaagaga agaaaagcac
    26941 tccaaaagtt agatgtttga tgtatctaac ttttggggtg tcattcaaaa tttagtggct
    27001 cttttttagt aaaatactat gatttcagtt ttaggataac ctcaaacagt atctaaaata
    27061 gctatgtcaa tgaaaaaagg ggttagcatt tttttcgtga caaatggtag tagaaggacc
    27121 atgcccagga taaactcggt aatgattagg taaggtgaat agttcttggc gaataccagt
    27181 aatgaggtcc tcaaagttac tggtaggtaa gtcagtacga ccgatagttt cacgaaatag
    27241 agcatcgcca gtaacgacta attcatctga gtggaaaaca aatgagactc ctcccaaaga
    27301 atgaccaggt gttggtaaaa ctgtgaattc aaaaccattt agttgatagg gttgctttaa
    27361 tttaaaaaag ttctctgccg gtcttgctat aacagtaatg atatcatcat gacggcctaa
    27421 gccagataaa ttatcatctg gggaagatag ccaagcggct tctttttctg aaacataaac
    27481 aggtggatga tcaaaagcat ctctgaccaa atctaaacta aaaatatgat catagtgagt
    27541 atgcgttaag aggatagcca ctaaaggttt ttcaaaggat ttaattttag caatgatttc
    27601 ttggccgtta cttcctggat caatgaggat aacagcttga tcattaacga ggtaataagt
    27661 attttcacca gcaatgtggt ttattgtttt atagattttc ataaggatag tgtaccaaaa
    27721 aatcaagact ttgttaaaaa aagagtagcg gaaaataaat ggcaattagt ttcagaaaat
    27781 atgagatctc acagcaattt cttgacaccg ttttcataaa ggtgtatcat aaaagtaatc
    27841 aataagccaa gcctaaagct gaacttagtt taagaacact gactttttga gagcaaccta
    27901 atcggttgac taaaaaatat gaggtggaag atatgtcaaa agttgctatt gttactggtg
    27961 ctggtcaagg aattggattt gctattgcga aacgtcttca tgcagacggc tttaaaatag
    28021 gcattttaga ctataatgaa gagactgctc aggctgcggt tgataaacta tcacctgagg
    28081 atactgttgc agttgtagca gatgtttcaa aacgtgacca agtttttgat gctttccaaa
    28141 aagttgtaga tacttttggt gatttgaatg ttgttgttaa caatgcaggt gttgccccaa
    28201 caacccctct tgacacgatt actgaagagc agtttgaaaa ggcttttgca atcaatgtcg
    28261 gaggaacgat ttggggaagc caagcagctc aaaaacattt tagagaatta ggtcatggcg
    28321 gaaaaattat aaatgcaacg tcacaagcag gatgtgaagg gaatcctaat ttgactgttt
    28381 atggtggtac aaaatttgca gttcgtggta ttacacagac tttggcaaag gatttggcat
    28441 cagaaggaat cacagtcaat gcttacgcac caggtattgt taaaacacct atgatgtttg
    28501 atattgccca tgaagttggt aaaaatgcag gcaaagatga tgaatggggt atggagcaat
    28561 ttgcaaaaga tatcactttg aaacgcttgt ctgaaccaga agatgttgct aatgcagtag
    28621 gcttcctagc gggagatgat tcaaattata tcactggaca aacaatagtt gtcgatggtg
    28681 gtatggtatt ccattaaaaa tgattaatac tgacactgtg agtgaaactc acagtgtttt
    28741 ttatacttct taaaaaatta tcctatcttg gttattggag tgatggcacc tttgagatat
    28801 agtcatgttt tgttttatag atatagcgtg ctataataga cttactatga cccagaaaaa
    28861 acttagaaaa tatgcagtag tggacttgga agctactgga gcaggtccca atgcttctat
    28921 tattcaggtt gggattgtta ttatccaagg aaataaaatt attgatagtt atgaaacaga
    28981 tgttaatcct catgaatctt tggatgagca tattgttcat ttgacaggta ttacggataa
    29041 gcagttggct aaagctccgg attttggtca agtggcgcat cacatttatc aattgattga
    29101 agattgtatt tttgttgccc acaatgtaaa gttcgatgca aatttattgg cagaagctct
    29161 ttttttggaa ggatatgagt taacaatacc acgtgttgat actgttgaat tagcccaact
    29221 atttttccct cgctttgaaa agtataacct tggtcaccta tctaggcagc tcaatattga
    29281 tttggcagaa gctcatactg ctattgcaga tgcaagagct acagctatac tgtttttaag
    29341 acttttacaa aaaattgaga gccttccaat tgagtgctta gaaagcttat tagtctattc
    29401 agatagcctg ctatttgaaa cagcaatggt cattcaagaa ggattagcca aagccaaacc
    29461 ttatgatccc aacaaataca ttaaaatcag acagattctc ttaccaaagg gctcaaaagc
    29521 tctcaagcct tatcaaatat caaaaagctt ccctattaat atggcactat taggtctgga
    29581 agaaaggcct aaacagactc aatttgctca gttaattgac gaagattacc atcagggagt
    29641 agctagtttt attgaagctc agacaggtat tggaaaaacc tacggctacc tgctacccct
    29701 tttggcaaaa gaagatcaaa accaaatcat tgttagtgtg ccaaccaaac ttcttcaaga
    29761 ccaattaatg gctggtgaag tagctgctat tcaagaacaa tttcacatcg cctgccatag
    29821 tttaaaaggg cctgccaatt acctaaagct agatagcttt gcagatagtt tggaccaaaa
    29881 tgaccagaac cgtttggtga atcgttataa aatgcaatta ttggtttggc tcttagaaac
    29941 taagacagga gaccttgacg aaatcaaaca aaagcagcgc tttgccgctt attttgagca
    30001 gcttaagcac gatggagaca ttaagcagtc ttctgaattt tacgattatg atttttggag
    30061 agttagttat gaaaaagcca aaacagcccg tctcctgatt accaaccatg cttatttttt
    30121 acaccgtatt caagatgaca aggactttac tagaaacaag gtcttggtct ttgatgaggc
    30181 ccaaaagctg atgctccaat tagatcaatt atcacgtcat cagcttaacc tgacggtttt
    30241 tttacaaaca atacaggcca agttaagcaa ccctttacct cttttagaaa aacgcttatt
    30301 ggaaagcttg tcgtttgaac tgggacaagt gtcaagtgat tactatcaaa ataaagagca
    30361 ccagcttgct catgattggt ctcgtatagc tggctacgcc aaagaattaa caggagcaga
    30421 ttatcaagag ttacaagcct tttttgctac ctcagatggt gactactggc ttagtagcga
    30481 aaagcaagag gagaagcgag tgacttatct aaacagtgct agtaaggcat ttattcattt
    30541 ccaacagctc ttaccagaaa cggtgaaaac ttattttgtt tctgccactt taaccatcag
    30601 ctcagaagta accttggcag acctattagg ttttgaagag tatctctatc atgtcattga
    30661 aaaggataaa aagcaagacc agttggtgct tgttgatcaa gaggcgccta tcgtgacaga
    30721 agtcagtgac caaatctatg tggaagcaat tgcaaaacga atcgaaagct tgaagcaaga
    30781 gggttatcct attttagtgc tttttaattc taaaaaacac ttgctgttgg tttcagacta
    30841 tctagaccaa tggcaggtgc ctcacttagc gcaggaaaag aatggaacag cttataatat
    30901 taaaaaacgg tttgatcaag gagagcaaac tattttgctt ggtttaggtt ctttttggga
    30961 aggagttgat tttattcaag cggatcgtat gattaccctt atcgcacgat tgccctttga
    31021 taacccagag gatttctttg ttaagaaaat gagtcattat ctattggaaa aaggtaaaaa
    31081 tcctttcaga gattacttct tgccaatgac tattttgcga ttaaaacagg ctattggtcg
    31141 cacgatgcgt cgaaaagatc aaaagtcagt ggttattatt ttggatcgtc gtctccttac
    31201 aaagtcctat ggacaagtta ttttggaagg tctcggtcag gaatttttga tttctcaaca
    31261 aaattttcac gattgtctag tagaaacgga ctgttttttg atataataga aaggatctat
    31321 tatatcagaa aaatttttaa tggcgttaag cacaatcaac taacatttaa attagtttga
    31381 acttttatta tgctctttta gacaactgtt caaatgtttt cgaattgttt ttttgataag
    31441 gacaataaat gaacaaattt ttggttttag taaacccaat tactgttctt ttcattagtg
    31501 tcagttgcta tcaaaacagc ttgtcatagt tcgtgcgatc agtagcgagt attggtaaaa
    31561 ctgaaaccgt agattatgaa aaaatgatct gttgatcatt aattaatctg aagataagaa
    31621 gtcgataaaa aggagatacg atgcccaaac tatcaaagcg agttttagaa atgaaagaga
    31681 gtgttactct ggctgcaggt gctagagcta aggctttgaa agcgcagggc catgatgtac
    31741 tgaatttaac tttaggagaa ccagattttt ttacacctaa gcacattcaa gacaaggcga
    31801 ttgaatctat tcaaaatggt acggctagct tttatacaaa tgcttctggg cttccagaat
    31861 taaaagctgc gattgccaca tatctgaaaa atcagtatgg gtatcacttg tctcctgatc
    31921 agattgtggc aggaacaggt gccaagttca tcctttatgc cttttttatg gctgttttaa
    31981 atcctggtga ccaagttctc attccaacac catattgggt atcttattct gaccaagtaa
    32041 aaatggcaga aggtcagccg atcttcgttc aaggactaga agaaaatcaa ttcaaggtaa
    32101 ctgttgacca gctggaaaga gcaaggacaa gtaagactaa agttgtcctt atcaattctc
    32161 cttccaatcc aacaggcatg atttatggtg ctgaggaatt aagggccatt ggagaatggg
    32221 cggtgcacaa cgatattctt attctggcag atgatattta cgggtcctta gtctataacg
    32281 gcaatcagtt tgttcctatt tcaacactct cagaagcgat tcgtcgtcaa accattacgg
    32341 tcaatggcgt ggctaaaagt tatgccatga caggctggcg ggttggtttt gcagcaggtg
    32401 aaccagagat catttcggca atgagtaaga tcatcgggca gactacctct aacctgacaa
    32461 ccgtttcaca atatgctgct atcgaggctt tctgtggtag tcagtcatca cttgaagaga
    32521 tgcgattagc atttgaagag cgccttaata tcacttatcc attgttgtgt caggtccctg
    32581 ggtttgaggt tgttaaacca caaggagctt tttacttctt tccaaacgtt aagaaagcca
    32641 tggaaatgac aggtttttcc gatgtgacaa gctttgccaa tgctatttta gaggaagtcg
    32701 gattagctgt tgtgagtgga gcaggctttg gcgcaccgga aaatgttcgt ctgagttatg
    32761 ctacagatat cgaaaccctt aaagaagctg ttcgtagact gcatgtcttt atggaaagta
    32821 atgaaattaa ttaaataaaa atagagagaa acttatgtca aaaaaactta tttcaattgt
    32881 tgatgttaaa gattatgttg gtcaagaagt tactattggt gcttgggtta ctaacaaatc
    32941 aggtaaaggt aagattgcgt ttgtacagtt gcgcgatggt tctgcttttt tccaaggtgt
    33001 cgcttttaaa ccaaacttca ttgaaaaata tggtgaagaa tcaggtcttg aaaaatttga
    33061 cgttattaaa cgtctcaatc aagaaacatc tgtttatgta acagggattg ttaaagagga
    33121 tgagcgttct aaatttggct atgagcttga tattactgac cttgaaatca ttggagagtc
    33181 acatgaatat cctataactc ctaaagagca tgggacagac ttcttgatgg acaaccgtca
    33241 cctttggttg cgttctcgta aacaaatggc tgttatgcaa attcgtaacg ctattattta
    33301 tgcaacttat gaattctttg accaaaatgg ttttatcaaa tttgatagcc caattttatc
    33361 agaaaatgca gcagaagatt caacagagtt attcgaaaca gactattttg ggaaaccagc
    33421 atttcttagc caatctggtc aattatacct tgaagctgga gcaatggctc taggccgagt
    33481 tttcgacttt ggtccagtct ttcgtgctga aaaatctaaa acacgtcgcc atttaacaga
    33541 attttggatg atggatgctg agtattcttt cttgtcacat gaggaatcac ttgaccttca
    33601 agaagcttat gttaaagcat tgatccaagg cgttctagat cgtgcgcctc aagcacttga
    33661 tattttagaa cgtgatgttg aggctctcaa acgttatatc acagagccat tcaagcgtgt
    33721 ttcttatgat gatgctatta cacttcttca agagcatgaa gcagatgaag atacagatta
    33781 tgaacattta gagcatggtg atgactttgg ttccccacat gaaacttgga tttctaatta
    33841 ctttggagta ccgacatttg tagtgaatta cccagcaagc tttaaagcat tttatatgaa
    33901 accagttcct ggaaatccag aacgcgttct ttgtgctgat cttttagctc cagaaggtta
    33961 tggagagatc attggcggct caatgcgtga agacaactac gatgctcttg tggctaaaat
    34021 ggatgaacta ggtatggata aatcagaata tgacttttac cttgaccttc gtaaatatgg
    34081 ctctgttcct catggcggtt ttggtatagg tattgaacgt atggtaactt ttgttgctgg
    34141 taccaaacat attcgcgaag ctattccatt cccacgtatg ttacatcgga ttagacccta
    34201 ataatacaaa aaagttcatg tcatctatga actttttgta ttggtaagaa gtgagtgtgt
    34261 tataaaaaca tcaatatgtc ctaaaggaga gataagaaat agtagagttt gaacctacat
    34321 cctttgtgaa gataaatttt ggaagtttag tagatcagtt agcagataag acaatatagc
    34381 tttgctcaag ggatatttta tagacaagta tttggactac gattatcaaa tagacactct
    34441 catttgatac taaaaaggtc tcgtctattc atataatagg agagctacgg gagttattat
    34501 taaaattagc tgatttttac agttcaaaat gtgttaccac tttttcactt cttagctttc
    34561 ttatgtatgt ggtataatga aggtggacgg ttcgatagag aaagggaaat tgtaaatgtc
    34621 agacaaacac attaatttag ttattgtgac aggaatgagc ggcgctggaa aaacagttgc
    34681 cattcagtct tttgaggatc taggctactt taccattgat aatatgcccc cagccttggt
    34741 tccaaaattt ttagaattaa ttgaacaaac caatgaaaat cgtagggtgg ctttggttgt
    34801 cgatatgaga agtcgtttgt ttttcaagga aattaattct accttagata gtattgaaag
    34861 caatcctagc attgattttc ggattctttt tttggatgca acggatggag aattggtgtc
    34921 acgctataaa gaaaccagac ggagccaccc tttggctgcg gacggtcgtg tgcttgatgg
    34981 tattcgattg gaaagagaac tcctatctcc tttgaaaagc atgagccaac atgtggtgga
    35041 tacaacaaaa ttgaccccta gacaattgcg taaaaccatt tcagaccagt tttctgaagg
    35101 gtctaatcaa ccctctttcc gtattgaagt gatgagcttt gggttcaaat atggtcttcc
    35161 tttggatgcg gatttggttt ttgatgtgcg ttttctaccc aatccttatt atcaggtaga
    35221 gcttcgtgaa aaaacaggac tagatgagga cgtttttaat tatgtgatgt ctcacccaga
    35281 atcagaggtg ttttacaagc atttgttaaa ccttattgtc cctatcttac cggcttacca
    35341 aaaagaaggg aagtctgtct tgacggtggc tattggctgc acaggaggcc aacaccgcag
    35401 cgttgccttt gcccattgct tggcagaaag tctggcaaca gattggtcgg ttaatgaaag
    35461 ccatcgtgat caaaatcgtc gtaaggaaac ggtgaatcgt tcatgaaaaa tcctaaaatg
    35521 actgttatag gtgggggtac tggcatctcc attatcctaa aaagccttcg taatgaagca
    35581 gttgatatta ctgctgtggt cactgttgca gatgatggtg gttcctcagg agaacttcgt
    35641 aatgccatgc aattagctcc gccaggagac ttacggaatg tcttgttagc gatgagtgac
    35701 atgccaaaat tttatgagcg ggttttccaa taccgtttta acgagagtga tggagccttg
    35761 gctggtcacc ctctaggcaa tctcattatt gctggtatct ctgaaatgca aggctcaacc
    35821 tacaatgcca ttcaaatttt aaccaaattt ttccatatta caggtaaaat ttacccctct
    35881 agcgagcagg ccttgaccct tcatgctgtt tttaaagatg ggcatgaagt agcaggagaa
    35941 agttctattg ccaaataccc aggcatgatt gaccatgtct atgtgaccaa cacctacaac
    36001 gaccaaaagc ctcaggccag tcgtaaggtg gttgaagcta ttctagaaag tgacatgatt
    36061 gtcttgggac caggctcact gtttacctct attttaccta atttggtcat tccagaaatt
    36121 aaagaagctt tgcgccagac taaagcagaa gtggtgtata tttgtaatat catgacccag
    36181 tatggtgaaa cagaacaatt ttcagatgca gaccatgtgg ctgttttaaa tcagcactta
    36241 ggaagagatt tgattgatac ggttttggta aatgtggcta aggtgccgca ggcttacatg
    36301 aattctaaca aatttgatga atacctcgtt caggttgatc acgactttgc tggcctttgc
    36361 agggctgcta agcgtgtgat ttcgtcttat tttttacggt tggagaatgg cggagctttt
    36421 cacgatggta acttagtggt ggaagaatta atgaatttag tgaggatagt caagcaatga
    36481 gttttacaac caaggtcaaa gaggaattaa ttcacttgtc cacaggtgac aacaatgaat
    36541 tggccgccat tatcaaatta tcaggcagct tgggcttagc tcaccaaagc cttcatttat
    36601 ccattaccac tgaaaatgct aaaattgccc gctacattta ttctcttatt gaagacgcct
    36661 atgttatcgt gccagaaatc aggtatcatc aaaagacgaa cctgcgcaaa aaccgtgttt
    36721 acaccgtcta tgttgagcaa ggtgtggaaa ccattttagc cgatttgaaa ttagcggact
    36781 ccttttttgg tctggaaaca ggcatcgaac ctcaggtttt atcagacgat aatgctggcc
    36841 gttcttatct taaaggggct tttctagcgg caggtagcat cagagatcct gagtcaggca
    36901 agtatcagtt agaaatctat tcagtttatc ttgatcatgc tcaagattta gctcaactca
    36961 tgcaaaaatt tatgctagat gccaaaacca tcgagcataa aagcggtgca gttacttatg
    37021 tgcaaaaggc agaagatatc atggactttt taattataat tggtgccatg tcctgcaaag
    37081 aagactttga agccatcaaa ttacttcgag aagctcgaaa tgatattaac cgtgccaata
    37141 atgcagaaac agctaatatt gccaaaacta tcagtgctag catgaaaacc attaataata
    37201 ttattaaaat catggacaca attggtttag aaagtttacc gattgaattg caacaggtag
    37261 cccagcttcg ggttaagcat ccagactatt ccattcaaca agttgctgat gctcttgagt
    37321 tcccaatcac caagagcggg gtcaaccatc gcttgcgtaa aatcaataaa attgcagatg
    37381 acttatagaa catttttatc gtatgagcct ttgttcatac gatttttttg tctaaaagtc
    37441 gttttccatt gtttttttga agtgtctgat gttctgtgat atgataaaag ggataataac
    37501 gtttgtaaag gagactgata atggcatgta caactatact agttgtagtg cctaaataat
    37561 gcttttaaaa cttaaaaata atatcgatgt tatccatagt aacctcgact ctatctatca
    37621 attgcttaac tatcctagat tgaacatcat acgaagattt tctaatgtca aggttatcca
    37681 tcagcattcc aagagagtct tcgtttttct tgtctaaata attcaattta attgctttgt
    37741 tataatcatc ttttaaatga ttgagttctt cgatgtcttt ttttagtttt ggtaggtcaa
    37801 tcaaatcatt aatatataaa tcgtttagac gattgatttt tttatcgatt
//
LOCUS       DAUDYX010000015        37141 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.015, whole genome shotgun sequence.
ACCESSION   DAUDYX010000015 DAUDYX010000000
VERSION     DAUDYX010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37141
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.015"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            14..393
                     /locus_tag="VHD02_000712"
                     /pseudo
     CDS             14..393
                     /locus_tag="VHD02_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017516.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(766..1892)
                     /locus_tag="VHD02_000713"
     CDS             complement(join(766..1658,1658..1892))
                     /locus_tag="VHD02_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEQ5497342.1"
                     /translation="MSTVKRYDEEFKQSLVNLYQTGKTQSELCKDYGVSASALAKWIK
                     QYSQVKLEDNSVLTAKQIQELQKRNAQLEEENLIPKKSKCHIHAKLKVRLLAVYRLRF
                     EHATTTLCRVLRVNRSTYYKFLKHKPSKRELDNQIYRKQILEIYTKANKRLGVKSIKV
                     ILQRDYDTKISEGRIYRLMKNMALPKMATVKPKTALKRTQKTYPQNLLNQKFNPDKPN
                     QVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHHMDAKLACDTLELALNKRKI
                     EGTLLFHSDQGSQFKARKLRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINR
                     KKYQNIKQVQLDCFEYIENFYNNYNPHTANLGLTPNQKEEN"
     gene            2196..2993
                     /locus_tag="VHD02_000714"
     CDS             2196..2993
                     /locus_tag="VHD02_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ5497343.1"
                     /translation="MIKLIATDMDGTFLAEDGTYNQEQLAALLPKLAEKGILFAVSSG
                     RSLLAIDQLFEPFLDQIAVIAENGSVVQYHGEILFADMMTKEQYTEVAKKVLANPHYV
                     ETGMVFSGQKAAYILKGASEEYIQKTKHYYANVKVINGFEDMENDAIFKVSTNFTGHT
                     VLEGSDWLNQALPYATAVTTGFDSIDIILKEVNKGFGMEHLCQALGIKKAETIAFGDN
                     FNDYQMLEFAGRAIATENARPEIKVISDQVIGHCNDGAVLTYLKGLV"
     gene            2997..3821
                     /locus_tag="VHD02_000715"
     CDS             2997..3821
                     /locus_tag="VHD02_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ5497344.1"
                     /translation="MNIRILALDLDGTLYNTEKIVTDANKKALAAAREKGVKVVITTG
                     RPLKAIGNLLEELDLLDHDDYSITFNGGLVQRNTGEVLDKSSLSFDQVCQIQQALEAV
                     GLPTDIISGGDVYSIPSKDGRHSQYHLANPLLTFIEVTSVAELPKDITYNKIVTVTDP
                     DFLDQQIIKLSPSLFEDFEAFKSRDIIFEIMPKGIDKAFGLNLLCQHLGLDARHVMAM
                     GDEANDFAMLEWAGLGVAMANGVSGAKAVADAVTTLTNDESGVAEAVKTFILEEES"
     gene            3821..5371
                     /gene="ftsY"
                     /locus_tag="VHD02_000716"
     CDS             3821..5371
                     /gene="ftsY"
                     /locus_tag="VHD02_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEQ5497345.1"
                     /translation="MGLFDRLFGKKETPKVAEEKLEENLLTETTQKEELSEKANEQDK
                     IEVVQQEDVSSEGAGSVENGPEAASVNAQVEEETGDNSNHSSEDTNEFAADKTDLKVS
                     ELSQSTASEPKDLVDQPVVEQFPTKQAQADASNDSANEEAVDTSKEQSSSQQVMEDYY
                     RRKAALEKSLQEKAAATVPVMPEEVPQENQASTSAEASQNKATYDTIPETDQEKYKRS
                     LKKTRTGFSARLNSFFANFRRVDEEFFEDLEEMLILSDVGVHVATTLTEELRYEAKLE
                     NAKKPDALKRVIVEKLVDIYEKDGRYNEAINYQDGLTVMLFVGVNGVGKTTSIGKLAY
                     RYKQEGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVITGPEKADPASVVFDGMEKAVA
                     KEVDILLIDTAGRLQNKENLMAELEKMGRIIKRVLPDAPHETLLALDASTGQNALSQA
                     KEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVKFIGFGEKVDDIGEFHSEDFM
                     KGLLEGIL"
     gene            complement(5426..6793)
                     /locus_tag="VHD02_000717"
     CDS             complement(5426..6793)
                     /locus_tag="VHD02_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ5497346.1"
                     /translation="MSHHQQTVSKQTIMAIIAIALIGFSGILSETSMNVTFPTLMSVY
                     QLPLNSLQWMTTIYLLAVAIMMTTSATLKKNVRERPLFFMATGLFTFGTILAVLTQSF
                     AIMLLARIFQGIGTGLVMPQMFNIILERVPMHKVGLFMGFAGLIISLAPAFGPTYGGF
                     MISHFNWQWIFICILPVPLIAGILAYYYLEDSPVSEKVPFDWLAFIALSISLTSALLA
                     ITSLENGSVNLYYLGLFILSLILFLYKNLTAKQPFLDIRILKIPSLTFGLIPFFVFQL
                     INLGINFLTPNFIVMEKIANSSQAGMVLLPGTLLGALLAPAFGKLYDQKGARLSLYLG
                     NALFSLSLIIMTLQTRHFMLLPFTLLYILFTFGRNMGFNNSLATAIRELPAEKNADAT
                     AIFQMMQQFAGALGTAMASLIANSQPEFTSGVQSVYLLFTIFALLDFIFFFAMFYHLG
                     KKGLA"
     gene            7121..7963
                     /locus_tag="VHD02_000718"
     CDS             7121..7963
                     /locus_tag="VHD02_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEQ5497347.1"
                     /translation="MLIKRVLNHNAAISTNHQGLDILLMGKGIAFGKKVGDSIELNAI
                     ETSFVLKNSDNMNRFTELFITVPQEVVACSERIINLGKIKLGKTLDEILYINLTDHIH
                     SAIERHEQGMLIHNPLRWEIQRYYPDEYSLGVKALELIERNLGVTLAIDEAAFIAMHF
                     VNASLDTPFKEPHRLTEIVSYIEQKIKTDFKTELDDTSIDYYRFMTHIKLFAQRVLSE
                     MSYDDDDAELLIVVKTKYPKEYRCVLDISEEIKKRYNYHLNSSELLYLTVHVKRLVKH
                     LKET"
     gene            7965..9827
                     /locus_tag="VHD02_000719"
     CDS             7965..9827
                     /locus_tag="VHD02_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEQ5497348.1"
                     /translation="MTYQETAKAILAAVGGKTNIQRVTHCVTRLRLVLKNDEKVKDQQ
                     VKSISNVIGVMRKNGQYQIILGNDVNNYYQAFLSLGHFDNQDEDHSSKAKGSILERLI
                     ETIAGVITPLIPALLGGGMLKVVGILLPMLGLASADSQTVAFINFFGDAAYYFMPVMI
                     AYSAAARFKVTPVLAATIAGILLHPAFVAMVAEGKPLALFGAPVTPASYGSSVIPILM
                     MVYLMQYIEKWVNRLVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGQGLSNTMLA
                     IYHVAPWLALAILGAIMPLVVMTGMHWAFAPIFLAASVATPDVLILPAMLASNLAQGA
                     ASLAVAFKTKQKQTRQVALAAGISALLAGITEPALYGVTLKFKKPLYAAMISGGLVGA
                     FIGFVNIASYTFVVPSIIGLPQYINPSGGANFTNALIAGAATIVLAFSLTWFMGIDEE
                     SPEQVSVAADMSQVKSGLSTKQTLYAPMTGEMLFLSEVPDETFSSKLLGEGFAILPSE
                     GEVYAPFDGEVITFFPTKHAVALKNTRGVEVLIHVGIDTVELKGQGFEQLVSVGDFVK
                     RGQALLKMDIDFITSKGYSLISPVVVTNSAEQLEIIIQDDKKIVTKEDALLVIL"
     gene            9846..11270
                     /locus_tag="VHD02_000720"
     CDS             9846..11270
                     /locus_tag="VHD02_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEQ5497349.1"
                     /translation="MGIFPKDFLWGGAVAANQVEGAFEADAKGLSVQDVLPNGGLGEW
                     TDSPTSDNLTLEAIDFYHRYKEDIALFAEMGFKVFRTSIAWSRIFPNGDDDQPNEAGL
                     QFYDDLFDELLNYGIEPLVTLSHYETPLHLAKAYNGWTDRRLIGFFERFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGGIMTEKEKLSLQDLYQAIHHELVASASVTKLAHEI
                     NPDVKVGCMILAMPAYPMTSDPRDILAAHAFENLNLLFSDIHVRGKYPSYIKSYFKEN
                     GIEIVFEDGDKELLAEHTVDFLSFSYYMSVTQAHNPEAYTSGQGNILGGLSNPYLESS
                     EWGWQIDPIGLRLVLNQYYDRYQIPLFIVENGLGAKDQLVQTADGSMTVHDDYRIDYM
                     SQHLVQVAKAIEDGVEVMGYTAWGCIDCVSMSTAQLSKRYGFIYVDRNDDGTGQLTRY
                     KKKSFDWYRQVIQTNGRYLEDNFS"
     gene            complement(11369..12184)
                     /locus_tag="VHD02_000721"
     CDS             complement(11369..12184)
                     /locus_tag="VHD02_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEQ5497350.1"
                     /translation="MIRFLILSGYFELTMYLQLSGKLDQYINVRYSYLAYISMVLSFI
                     LALVQLYTWMKNIKVHSHLTGKIARLTSPFILVFPVLIGLLVPTVTLDSTTVSAKGYT
                     FPLAAGASKTGVSDDGTTIQYLKPDTSLYFTKSAYQKEMRQELHKYKGKKPVTITTEN
                     YMEVMELIYLYPDEFLDRDIQYTGFVYNEPGHDNYQFLFRFGIIHCIADSGVYGLLTT
                     GNQTSYPNNTWLTVKGRLHMEYDKNLEQHLPVLQLAEVHQTKEPNNPYVYRVF"
     gene            complement(12184..13086)
                     /locus_tag="VHD02_000722"
     CDS             complement(12184..13086)
                     /locus_tag="VHD02_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEQ5497351.1"
                     /translation="MSLFSNLPPSVLQWFAIFMSIIIEALPFVLLGTILSGCIEVFVT
                     PELVQKYLPKQKCLRILFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSLRFLILRLVGAALVAITLGVMLAFIVDDNILKDNAQPVHFH
                     DYSHESLPKRIYLALVHAIDEFFDTGRYLVFGTLIASAMQIYVPTRVLTTIGHNPLTA
                     ILIMMLMAFILSLCSEADAFIGASLLSTFGVAPVLAFLLIGPMVDIKNLMMMVKAFKG
                     RFIVQFIGVSVLMIAVYCLLVGVL"
     gene            complement(13234..13389)
                     /locus_tag="VHD02_000723"
     CDS             complement(13234..13389)
                     /locus_tag="VHD02_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497352.1"
                     /translation="MAITHKKNDELEKMLAGFASIPSFDRPLEVNTDGKLATKEKTTE
                     QTKPSKP"
     gene            13623..15755
                     /locus_tag="VHD02_000724"
     CDS             13623..15755
                     /locus_tag="VHD02_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEQ5497353.1"
                     /translation="MENNNNHNIAEALSVSLHQIEQVLALTAQGNTIPFIARYRKEVT
                     GNLDEVVIKSIIDMDKSLTTLNERKATILAKIEEQGKLTDQLRTSIEATEKLADLEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNAQNLETAAEPFVTEGFASPQEALAGAVD
                     ILVEAMSEDAKLRSWTYNEIWQYSRLVSTLKDEQLDEKKVFQIYYNFSDQVSNMQGYR
                     TLALNRGEKLGILKVSFEHNLEKMQRFFSVRFKETNPYIEEVINQTIKKKIVPAMERR
                     VRSELSDAAEDGAIHLFSENLRHLLLVSPLKGKMVLGFDPAFRTGAKLAIVDQTGKLL
                     TTQVIYPVAPASQTKIQAAKETLTQLIETYQIDIIAIGNGTASRESEAFVADVLKDFP
                     NTSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLSENLGFVVDTVVNQVGVNVNTASPSLLAHVSGLNKTISENIVKYR
                     EENGALTSRADIKKVPRLGAKAFEQAAGFLRIPGAKNILDNTGVHPESYPAVKELFKV
                     LGIQDLDDAAKATLAAVQVPQMAETLAIGQETLKDIIADLLKPGRDLRDDFEAPILRQ
                     DILDLKDLEIGQKLEGTVRNVVDFGAFVDIGVHEDGLIHISEMSKTFVNHPSQVVSVG
                     DLVTVWVSKIDLDRHKVNLSLLPPRDTH"
     gene            15742..16179
                     /locus_tag="VHD02_000725"
     CDS             15742..16179
                     /locus_tag="VHD02_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEQ5497354.1"
                     /translation="MTLTNYVQEVSLADFGKPFHHKAYWNKRLKTTGGRFFPKDGHLD
                     FNPRMLEEHGELIFRKIVRHELCHYHLYFEGRGYHHKDRDFKDLLAQVNGLRYVPTSS
                     KSKTNHHYSCQTCGQVYQRKRRINLAKYVCGNCHGKLMEKNQS"
     gene            16243..16515
                     /locus_tag="VHD02_000726"
     CDS             16243..16515
                     /locus_tag="VHD02_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEQ5497355.1"
                     /translation="METKFYKQRKNRLVAGVIAGLADKYGWDLALARVLAALLIYGTG
                     FGVLLYILLAIFLPYKEDLLEERYGRGPRRRKDADVLNEEEDGWFW"
     gene            16880..17812
                     /locus_tag="VHD02_000727"
     CDS             16880..17812
                     /locus_tag="VHD02_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEQ5497356.1"
                     /translation="MTVTVKMLVQKVKLDVVYATDNLLSKEITTSDISRPGLEMTGYF
                     DYYAPERLQLFGMKEWSYLTQMTSHNRYSVLKEMFKKDTPAVVVSRNLAIPKEMVQAA
                     KEEGISLLSSRVSTSRLAGEMSYFLDASLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAIYLENFEAGKVFDRLGNGNEEITFSGVRIPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKEMGFDATKTFEDRLTQLITKNEVSQ"
     gene            17809..18588
                     /locus_tag="VHD02_000728"
     CDS             17809..18588
                     /locus_tag="VHD02_000728"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEQ5497357.1"
                     /translation="MINPIALKCGPLAIHWYALCILSGLVLAVYLASKEAPKKGISSD
                     AIFDFILIAFPLAIVGARIYYVIFEWSYYVKHLDEIIAIWNGGIAIYGGLITGALVLL
                     AYCYNKVLNPIHFLDIAAPSVMVAQAIGRWGNFINQEAYGKAVSQLNYLPSFIQKQMF
                     IEGSYRIPTFLYESLWNLLGFVIIMMWRRKPKSLLDGEIFAFYLIWYGSGRLVIEGMR
                     TDSLMFLGIRISQYVSALLIIIGLIFVIKRRRQKGISYYQE"
     gene            18610..19017
                     /locus_tag="VHD02_000729"
     CDS             18610..19017
                     /locus_tag="VHD02_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEQ5497358.1"
                     /translation="MDLVGISLMIIALGFVALVIFLIIVLKKVSETIDEAKKTISVLT
                     SDVNVTLHQTNDILAKANILVEDVNGKVATIDPLFVAIADLSESLSDLNSQARHFGQK
                     ATNATGNVSKAGKLALVGKVASKVFGKKGEKHE"
     gene            19010..19438
                     /locus_tag="VHD02_000730"
     CDS             19010..19438
                     /locus_tag="VHD02_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEQ5497359.1"
                     /translation="MNKSFKNLVIGAVSGVAAAYFLSTEKGKALKNRAEKAYQAYKES
                     PDDYHQFAKEKGSEYSHLARDTFYDVKDKLASGDLTKEDMLDLLKDKTTAFVQKTKET
                     LAEVEAKEKQDDVIIDLNEEDIIIDYTEQDEPVSDTLDKH"
     gene            complement(19598..19882)
                     /locus_tag="VHD02_000731"
     CDS             complement(19598..19882)
                     /locus_tag="VHD02_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEQ5497360.1"
                     /translation="MPAPLKKHNSVEQDDSYQESQSKFRDFQEFNGQSAKLKELVFFA
                     RIASFSLITVLFAFVLLVMNLAPMFAFLFASIISLGITSLLAKVIKSLLM"
     gene            20081..21007
                     /locus_tag="VHD02_000732"
     CDS             20081..21007
                     /locus_tag="VHD02_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEQ5497361.1"
                     /translation="MEKIIITATAESIEQVKALLAAGVDRIYVGEANYGLRLPHNFSY
                     DELRQIAKLVHDAGKELTVACNALMHQDMMDQIKPFLDLMIEIAVDYLVVGDAGVFYV
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVESVLAREIPSAELFTLAENLEFPAE
                     VLVYGASVIHHSKRPLLENYYHFTKIDDEVSRERGLFLAEPGDASSHYSIYEDNHGTH
                     IFINNDIDMMSKLGELYAHGLTHWKLDGIYCPGDDFVAITKLFIQAKTLLEAGQFTQE
                     EAEKLDQAVHAHHPAGRGLDTGFYEFDPKTVK"
     gene            21109..22395
                     /locus_tag="VHD02_000733"
     CDS             21109..22395
                     /locus_tag="VHD02_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEQ5497362.1"
                     /translation="MSHMKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGIDYAHARGAKVYVAANMVTHEGNEIGAGEWFRQLRDMGLDAVIVSDP
                     ALIVICSTEAPGLEIHLSTQASSTNYETFEFWKAMGLTRVVLAREVNMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGGERRSL
                     KGEIPEDYSMSSVDMCMIDHIPDLIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFYAIKEELIDELWKVAQRELATGFYYGIPTENEQLFGARRKIPQYKFVGEVV
                     AFDSASMTATIRQRNVIMEGDRIECYGPGFRHFETVVKDLHDADGQKIDRAPNPMELL
                     TISLPREVKPGDMIRACKEGLVNLYQKDGTSKTVRT"
     gene            22605..22817
                     /locus_tag="VHD02_000734"
     CDS             22605..22817
                     /locus_tag="VHD02_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEQ5497363.1"
                     /translation="MAEFTFNIEEHLLTLSENDKGWTKELNRVSFNGAEAKWDIRTWS
                     PDHTKMGKGITLTNEEFKTILDAFRK"
     gene            complement(22914..23054)
                     /locus_tag="VHD02_000735"
     CDS             complement(22914..23054)
                     /locus_tag="VHD02_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497364.1"
                     /translation="MTKEYISYISATIFLLYGLLAAKWIFLGLGLILVLVGLADRLNS
                     KK"
     gene            complement(23190..24683)
                     /gene="lysS"
                     /locus_tag="VHD02_000736"
     CDS             complement(23190..24683)
                     /gene="lysS"
                     /locus_tag="VHD02_000736"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEQ5497365.1"
                     /translation="MSNQHIEELNDQQIVRREKMMALAEQGIDPFGKRFDRTANSAEL
                     KEKYADKTKEELHELNETAIVAGRLMTKRGKGKVGFAHLQDREGQIQLYVRKDSVGED
                     NYEIFKKADLGDFIGVEGEVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETI
                     YRKRHLDLISNRESFDRFVTRSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFV
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADYLDIMNLTEGIIQHAAKAVKGDGPIDYQGTEIRINEPFKRVHMVDAIKEVTGVD
                     FWPEMTVEEAIALAKEKQVPLEKHFTSVGHIINAFFEEFVEETLVQPTFVFGHPVEVS
                     PLAKKNPEDTRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQAQAKELGDDEATG
                     IDYDFVEALEYGMPPTGGLGIGIDRLCMLLTNTTTIRDVLLFPTMKP"
     gene            24857..25759
                     /locus_tag="VHD02_000737"
     CDS             24857..25759
                     /locus_tag="VHD02_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEQ5497366.1"
                     /translation="MITAIVFDVDDTIYDQQAPYRIAMEKCFPDFDMSVMNQAYIRFR
                     HYSDVGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEAAGVHFQEVYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVRKLGLYDYIDPKHVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPQTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRQLKPGTKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDINDKKNPILQMGINNGLMMAAERLLESNMS
                     IDKVVILLRLTKQQEKVLRLKYAK"
     gene            complement(25867..26490)
                     /locus_tag="VHD02_000738"
     CDS             complement(25867..26490)
                     /locus_tag="VHD02_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEQ5497367.1"
                     /translation="MKLYFVRHGKTLWNLEGRFQGAGGDSPLLEEAKDEIHLLGKELA
                     KVDFDAVYTSDLQRAMATAAIILDAFDQQPKLYHTDQLREWRLGKLEGAKIATMAAIY
                     PQQMLAFRENLAQFKPDQFEAESIYQTTQRVCHLIQSFKDKHYQNVLIVGHGANLTAT
                     IRSLLGFEPALLLAKGGLDNTSLTILETKDYLTYDCLIWNDKSFIEV"
     gene            complement(26831..27310)
                     /locus_tag="VHD02_000739"
     CDS             complement(26831..27310)
                     /locus_tag="VHD02_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEQ5497368.1"
                     /translation="MAKKTKLKKTLVEQILDKANIAHQGLKLNALEGDFPDDLQPSDI
                     YKTLALTGDQTGPLIGIIPLTEHLSEKQLAKVSGNKKVSMVPQKDLQKTTGYIHGANN
                     PVGIRQKHSYPIFIDQTALEKGQIIVSAGEVGRSIKISSQALADFVGASFADLKKRK"
     gene            complement(27401..27964)
                     /gene="thiT"
                     /locus_tag="VHD02_000740"
     CDS             complement(27401..27964)
                     /gene="thiT"
                     /locus_tag="VHD02_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEQ5497369.1"
                     /translation="MSPNTNVKYLIEAAIFAALAMTLSFIPDFAGWFSPSYGAIALVI
                     FSLRRGLKYGMLAGLIWGLLHFVLGKVYYLSMSQVFIEYILAFTSMGLAGSFSDSLIK
                     TLRRQQTFFAVFLAIMASLLAVTVRYLWHFLAGIIFWGSYAPKGMSAVWYSFSVNGTA
                     GVLTFLITCLALMIALPIHPQLFDPKD"
     regulatory      complement(28009..28105)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(28233..29081)
                     /locus_tag="VHD02_000741"
     CDS             complement(28233..29081)
                     /locus_tag="VHD02_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEQ5497370.1"
                     /translation="MRRKIKPIVVLVFFILLAMVLIIGKRQANHAKQKEVEDAKSHIP
                     IATSNPSKAKTSTSETEDFILNPIVDVSGWQLPEEIDYDTLSRHISGAIVRVYGGSQI
                     TAHNNAAFTTGIDKSFKTHIKEFQKRNVPVAVYSYALGRSTKEMKEEARAFYKNAAPY
                     NPTYYWIDVEEATMKDMNKGVTAFREELKKLGAENVGLYIGTYFMAEQDISTKGFDAV
                     WIPTYGSDSGYYEAAPNTTLDYDLHQYTSQGYLSGFNNALDLNQIAVTKDTKKTFEKL
                     FGNSNN"
     gene            29406..29909
                     /locus_tag="VHD02_000742"
     CDS             29406..29909
                     /locus_tag="VHD02_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ5497371.1"
                     /translation="MSAKLMSRIAIMSALCVVLRMVFSSLPNVQPVTAFLLSYLLYFG
                     LAEAVLVMMLCLFLSAFLLGFGPWVFWQVTCFVLVLLLWRFALYPLSQQFPKYQLGCQ
                     AFLVALCGLLYGVLIDTCFAYLYSMPWWSYVLAGMPFNIAHALSTLVFFPVVMMLFRR
                     LIGEQKI"
     gene            29893..30276
                     /locus_tag="VHD02_000743"
     CDS             29893..30276
                     /locus_tag="VHD02_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEQ5497372.1"
                     /translation="MNKRFKTGFLALVAMLLVACSQGTKQTQTPSVPKADHHVRLVVK
                     EDTNTIDEKVSFGKGDTVLEILKDNYEVKEKDGFITAIDGIEQDTKANKYWLFKVNGK
                     MADKGADQITVKDGDSIEFYQEVFK"
     gene            complement(30322..30801)
                     /locus_tag="VHD02_000744"
     CDS             complement(30322..30801)
                     /locus_tag="VHD02_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEQ5497373.1"
                     /translation="MPNLYDFTVKAQNGNDVSLAAYKKKVVLIVNTATKCGLTPQYQA
                     LQALYDTYHDKGFEVLDFPCNQFLNQAPGDAEEINHFCSLTYHTTFPRFAKIKVNGKD
                     ADPLFTWLKEEKSGPLGKRIEWNFTKFLIDQNGQVIKRYSSKTDPKLIEEDLKALLG"
     gene            complement(30794..32593)
                     /gene="pepF"
                     /locus_tag="VHD02_000745"
     CDS             complement(30794..32593)
                     /gene="pepF"
                     /locus_tag="VHD02_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEQ5497374.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEKTLQDIDLFKRN
                     YEGRLTSVDDFTQALIEIEHIYIQMSHIGTYAFMPQTTDFSDESFAQIAQAGDDFMTK
                     ASVALSFFDTALANADLDVLDTLEKNPYFSAAIRMAKIQKEHLLSPDVEKALANLREV
                     INAPYDIYTKMRAGDFDMEDFEVDGKTYKNSFVSYENFYQNHENAEIREKAFRSFSKG
                     LRKHQNTAAAAYLAKVKSEKLLADMKGYASVFDYLLAEQEVDRSLFDRQIDLIMTEFG
                     PVAQKFLKHVAQVNGLEKMTFADWKLDIDNELNPEVSIDGAYDLVMKSLAPLGQEYTK
                     EIERYQTERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNTHMSTYYVEAPSTFNELMLSDYLEHQFDDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGGTFGADQLNAMMKEVLTDFWGDAVDIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVMSTAGYLNLKNNPDGAKEWLDFLKSGGSRTPLDTAMLIG
                     ADIATEKPLRDTIQFLSDTVDQIINYTEEMSHA"
     gene            32752..35550
                     /gene="ppc"
                     /locus_tag="VHD02_000746"
     CDS             32752..35550
                     /gene="ppc"
                     /locus_tag="VHD02_000746"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEQ5497375.1"
                     /translation="MIEETSKEGDGHLPLKKLESSNNQAIIAEEVALLKEMLENITRR
                     MIGDDAFTVIESIMVLSEKQDYIELEKVVANISNQEMEVISRYFSILPLLINISEDVD
                     LAYEINYQNNTDTDYLGKLALTIKDLAGKDNGKDILEQVNVVPVLTAHPTQVQRKTIL
                     ELTTHIHKLLRKYRDAKAGVINLEKWRQELYRYIEMIMQTDIIREKKLQVKNEIKNVM
                     QYYDGSLIQAVTKLTTEYKNLAQKHGLELDNPKPITMGMWIGGDRDGNPFVTAETLCL
                     SATVQSEVILNYYIDKLAALYRTFSLSSTLVQPNSEVERLASLSQDQSIYRGNEPYRR
                     AFHYIQSRLKQTQNQLTNQPAARMSSSVGLNTSAWSSPASLENPILAYDSPVDFKADL
                     KAIEQSLLDNGNSALIEGDLREVMQAVDIFGFFLASIDMRQDSSVQEACVAELLKGAN
                     IVDDYSSLSETEKCDVLLQQLMEEPRTLSSAAVAKSDLLEKELAIYTTARELKDKLGE
                     EVIKQHIISHTESVSDMFELAIMLKEVGLVDQQRARVQIVPLFETIEDLDNARDIMAA
                     YLSHDIVKSWIATNRNYQEIMLGYSDSNKDGGYLASGWTLYKAQNELTAIGEEHGVKI
                     TFFHGRGGTVGRGGGPSYDAITSQPFGSIKDRIRLTEQGEIIENKYGNKDVAYYHLEM
                     LISASINRMVTQMITDPNEIDSFREIMDSIVADSNTIYRKLVFDNPHFYDYFFEASPI
                     KEVSSLNIGSRPAARKTITEITGLRAIPWVFSWSQNRIMFPGWYGVGSAFKRYIDRAQ
                     GNLERLQHMYQTWPFFHSLLSNVDMVLSKSNMNIAFQYAQLAESQDVRDVFYEILDEW
                     QLTKNVILAIQDHDDLLEDNPSLKHSLKSRLPYFNVLNYIQIELIKRWRNNQLDENDE
                     KLIHTTINGIATGLRNSG"
     gene            35690..36994
                     /locus_tag="VHD02_000747"
     CDS             35690..36994
                     /locus_tag="VHD02_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEQ5497376.1"
                     /translation="MIISRSRGKTMKIDKRHLLNYSILLPYLILSVIGLIMVYSTTSV
                     SLIQAHANPFKSVINQGVFWIISLVAITFIYKLKLNFLTNTRVLTVVMLGEAFLLIIA
                     RFFTTAIKGAHGWIVIGPVSFQPAEYLKIIMVWYLALTFAKIQKNISLYDYQALTRRK
                     WWPTQWNDLRDWRVYSLLMVLLVAAQPDLGNASIIVLTAIIMFSISGIGYRWFSAILV
                     MITGLSTVFLGTIAVIGVERVAKIPVFGYVAKRFSAFFNPFHDLTDSGHQLANSYYAM
                     SNGGWFGQGLGNSIEKRGYLPEAQTDFVFSVVIEELGLIGAGFILALVFFLILRIMNV
                     GIKAKNPFNAMMALGVGGMMLMQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVA
                     VGFVLNIDASEKRDDIFKEAELSYRKDTRKENSKVVNIKQFQ"
BASE COUNT        11459 a         6567 c         7866 g        11249 t
ORIGIN      
        1 acattattca agtatgaact ataaaatctt catacgaact aagtcaggtg acatatttga
       61 tattgctgat ggtggtagaa ttgatgatat ggtttctaaa tttaataaag taaacgtatt
      121 aggagtttgc atgggaatag gaaccactgt tttatcacaa gagattgagt atgaaattga
      181 agatagaata atgatattag ttgaaaaaat agatgtgaaa atctataaga attgtcttga
      241 attagcaaat aaattaagtg ggtatcaaag tttttttaac atgaattata ttcgagacat
      301 cattatatta ttgttcgatt agatggaagt atggaataca gattttcatc tgtagcttta
      361 aaaaataaaa ttaaaagcat acttgatagt tgatgtatac tgtcatttag aataataatc
      421 acatattgtt gtcaacgaaa cccaccatag tctataatga aagcctagta gggatgagct
      481 atataccaac tatctatcaa tccagacgtc tctactaaaa aatactccgt cttctatgaa
      541 actagaaacg gagtatttct gtttgtctat accactaaat gaacagttat atagaaaggt
      601 gattggatgt ttggaataaa catcatgaaa aaaatttgag accttcttat atcctaaact
      661 agaacgaatg tctagtgaaa taaaaaaaaa acgacaccat taggatgtat tttggactaa
      721 tgtcaaataa gtagacagaa aaccgtgtta ttttattacg ttaaactaat tttcttcttt
      781 ctgattaggg gttagtccta gattagccgt atgtgggttg taattgttat aaaaattctc
      841 aatgtattca aagcagtcta attgaacctg tttgatattt tgatactttt ttcggttgat
      901 ttgtctatgc tttaaatact tgaaaaatgc ttcagttacg gcattatcat aaggatatcc
      961 aggtttagaa aaagaatgca tgatagtgtt gtcatcaatt atttttctca atttcctggc
     1021 cttaaattgt gacccttggt cggaatgaaa gagaagtgtt ccttcaatct ttcttttatt
     1081 aagagctaat tctagagtgt cacatgctaa ctttgcatcc atatgatgac tcagtttcca
     1141 agcaatacat tttctagaat agagatcaag tattgcgcag agatagacat atttcttata
     1201 tccaatagaa atataggtga agtcagtaga ccatacttga tttggtttgt caggattaaa
     1261 tttctggttg agtaagtttt gaggatacgt tttttgagtc cttttaagag ctgtttttgg
     1321 cttaacggta gccattttag ggagcgccat attcttcata agacggtaaa ttcttccttc
     1381 agagattttt gtgtcgtagt ctctttgaag aatgaccttg atagacttca caccaagtct
     1441 tttgtttgct ttggtataaa tctcaagtat ttgttttcta taaatttgat tatccaattc
     1501 tcttttggag ggcttatgtt ttagaaattt atagtaagtg gagcgattga cacgtaaaac
     1561 acgacataag gttgttgtgg cgtgttcaaa gcgtaaccga tagacagcga ggagtcttac
     1621 tttaagtttt gcatgaatat ggcacttgct ttttttagga taaggttctc ctcttcaagc
     1681 tgcgcattac gtttttgtag ctcttgtatt tgcttggcag taagtacaga attatcttcg
     1741 agtttgactt gagaatactg tttgatccat tttgcaagcg cagaagcgga taccccatag
     1801 tctttacaga gttcagactg agtttttcca gtttgataaa ggttgacaag agattgtttg
     1861 aattcttcat cgtagcgttt aactgttgac ataggttgtc ctttcgtttt tgtgtctttt
     1921 aaaacagatt gtaacacaca aagttttgtc tacttttata gtatacatcc agtggtcatt
     1981 tataaaaata aaaaagtagc agttcacttc aaaaacggtc aggttatcga aatataatct
     2041 ggctgttttt tacgaatgga atgaaagctg ctttttatga tataataaga aaaattgaac
     2101 agaatgcaga aatgatgggg cttatttaaa tcgttaaaaa gtgcacaccc taaatggcgt
     2161 ttgcaccccc ctagagtaaa aaaaggagac attgtatgat taaactaata gcaacagata
     2221 tggatggtac cttcctagct gaagatggta cttataatca agagcaacta gctgcgctct
     2281 tgccaaagtt agccgaaaaa ggcattttat ttgcggtttc gagtggccgc tcgctcttag
     2341 ccattgatca gctctttgag ccctttttgg atcagattgc tgttattgca gaaaatggtt
     2401 ccgtggttca ataccatggt gagattctat ttgcagatat gatgaccaaa gagcagtata
     2461 ccgaagttgc aaaaaaggtt ttagccaatc ctcactatgt agaaacaggt atggtttttt
     2521 ctgggcaaaa ggcagcctat attttaaaag gcgcatcaga agagtacatt caaaaaacaa
     2581 aacattacta tgctaatgtt aaggttatta atggttttga agatatggag aatgacgcca
     2641 tttttaaagt ttctaccaac ttcacaggcc ataccgtctt agaaggaagc gattggttga
     2701 atcaagccct cccttatgcc actgcagtga ccaccggctt tgattccatt gatattatct
     2761 taaaagaggt gaataagggc tttggtatgg agcatctttg tcaagccttg ggcattaaga
     2821 aagcagaaac gattgctttt ggggataatt ttaatgatta ccagatgttg gagtttgctg
     2881 gtagggctat tgctacggaa aatgcaagac ctgaaatcaa agtgatttca gaccaagtca
     2941 ttggccactg taatgacgga gcagtactaa cgtatttaaa aggactagtg taactcatga
     3001 atattagaat tttggcgctt gatttagacg gtaccctcta caacactgaa aaaattgtca
     3061 cagatgccaa taaaaaagcc ttagcagcgg ctagagaaaa aggggttaag gtggttatta
     3121 ccacaggtcg ccctctaaag gccatcggta acttgctaga agagttggac ttgcttgacc
     3181 acgatgacta cagtattacc tttaatgggg gtttggttca gcgaaataca ggggaggttt
     3241 tggataagag ctctctcagt tttgaccagg tttgccagat tcagcaagct ttagaggcgg
     3301 ttggcttacc aacagatatt atcagtggtg gggatgtgta tagcattcca agtaaggacg
     3361 gtaggcattc gcagtaccat ctagctaacc cactgttgac ctttattgag gtgacatctg
     3421 tagcggaact accaaaagac attacctata acaaaattgt gacagtcacc gatccagact
     3481 ttttggatca gcagattata aagctatccc caagcttatt tgaggatttt gaagccttta
     3541 aatcacgaga tattattttt gaaatcatgc caaaaggcat tgataaagct tttggattaa
     3601 acttgctttg tcagcacctt ggccttgacg ctagacatgt catggctatg ggagacgaag
     3661 ctaacgactt tgcgatgtta gaatgggcag gacttggagt agctatggct aatggtgttt
     3721 caggagctaa agccgttgcc gatgccgtga ccaccttgac caatgatgag tctggggttg
     3781 cagaagctgt gaagacattt attttagaag aggagagtta atgggattat ttgatcgttt
     3841 atttgggaaa aaagaaaccc cgaaggtagc tgaggaaaag ttagaagaaa atcttttgac
     3901 agaaacaact cagaaagagg agctttctga aaaagccaat gagcaagaca agattgaggt
     3961 ggttcaacaa gaggacgttt cttcagaggg cgcaggttcg gtggaaaatg gtcccgaagc
     4021 agcttcagta aatgcacagg ttgaggaaga aacaggtgat aatagtaatc attcatcaga
     4081 agataccaat gagtttgctg ccgacaaaac agaccttaag gtaagtgaac ttagtcaatc
     4141 tacggcatca gaaccaaaag accttgttga ccagccagtt gtggaacagt ttccaacaaa
     4201 acaagcccaa gctgatgcct caaatgattc ggcgaatgaa gaagcagtag acacttcaaa
     4261 agagcaatcc agtagccaac aggttatgga agactactac cgtcgtaagg ctgctcttga
     4321 aaaaagcctt caagaaaaag cagcggcaac ggttccagtg atgccagaag aagttccaca
     4381 agaaaatcaa gcctctacct cagcagaagc ttctcaaaac aaggcaacct acgataccat
     4441 tcctgagaca gatcaagaaa aatacaagcg tagcttgaaa aaaacgcgga ctggcttctc
     4501 agctcgtctg aacagtttct ttgccaattt caggcgagtg gatgaagaat tctttgaaga
     4561 cttggaagaa atgctgatct tatcagatgt cggtgtacat gtggcgacaa ctttgacaga
     4621 ggagttgcgt tatgaagcca aactggaaaa tgcgaaaaaa ccagatgcct tgaaacgtgt
     4681 gattgtggaa aaattggtag atatctatga aaaagacggg cgttataatg aagccattaa
     4741 ttatcaagat ggcttgacgg ttatgctttt tgtaggtgtt aacggggttg gtaaaacgac
     4801 ttcaatcggg aagctggctt accgttacaa acaagaaggt aaaaaagtca tgttggtggc
     4861 agcagatacc ttccgagcag gggctgtggc tcagctagtc gaatggggac gccgagtaga
     4921 tgtgccagtc attactggcc ctgaaaaggc tgatccagcg tctgttgttt ttgacgggat
     4981 ggaaaaagct gtggcaaaag aagtagatat tcttctgatt gatacggctg gtcgcctgca
     5041 gaacaaagaa aatctcatgg cagagctgga aaaaatgggt cgtatcatta agcgcgttct
     5101 accagatgcc cctcatgaaa cgcttttggc cttagatgcg tcaacaggtc aaaatgcgct
     5161 tagccaagcc aaggaatttt caaaaataac acctttaaca ggcttaattt taacgaaaat
     5221 tgatgggact gcaaaaggtg gggtggtcct agccatccgt caagaattag acattccagt
     5281 caagttcatc ggatttggtg aaaaagtgga tgatattgga gaattccact cagaagactt
     5341 tatgaaaggt cttttagaag gtattttata agaaaaaaaa ccagtcttgt tcaacagaac
     5401 gaggctggtt tttaagtaag tcagcttagg caagtccttt tttccctaaa tggtaaaaca
     5461 tagcaaagaa aaagataaaa tcaagtagag caaaaatagt aaagagaagg tagacagact
     5521 ggacaccgct tgtgaattct ggttgactat ttgctatcag tgatgccata gcagttccta
     5581 gagcgccagc aaattgctgc atcatctgaa aaatggccgt ggcatcggca tttttctcgg
     5641 caggcaattc tcgaatagcc gtggctaagc tattattaaa gcccatgtta cgcccaaacg
     5701 tgaataaaat atataaaaga gtgaatggta aaagcataaa atgtcttgtt tgaagtgtca
     5761 taataatcaa tgataaacta aataaggcat ttcctaaata aagcgaaagt cttgctcctt
     5821 tttgatcata aagtttacca aaagcaggtg ctagtagagc tccgagtaag gtaccaggta
     5881 gtaataccat accagcttga gaactattcg caatcttttc catgacaata aagtttggcg
     5941 ttagaaaatt tatgcctaaa ttaatcagct ggaagacaaa aaagggaatc aggccaaagg
     6001 ttagagaggg aattttgaga atgcgaatat caagaaaggg ttgcttagct gtgagattct
     6061 tgtagaggaa gaggataagg ctgagaataa aaagccctaa gtaatacaaa ttaacacttc
     6121 cgttttctag actagtaata gccaataagg cagaagttaa gctaatcgat agtgcaataa
     6181 atgccaacca gtcaaagggt actttttcgc ttactggaga atcttcgagg tagtaataag
     6241 ctagaatacc tgcaatcagt ggtacaggga gtatacagat aaaaatccat tgccaattaa
     6301 aatggctaat cataaagccc ccataagtag gcccaaaagc aggtgctaag ctaataataa
     6361 gaccagcaaa tcccataaat agacctacct tatgcattgg gacacgctct aaaataatat
     6421 taaacatctg aggcattacc agaccagtac caatgccttg aaaaatgcgg gctagcaaca
     6481 tgatcgcaaa ggactgggtc agaacggcca agatggtgcc aaatgtaaaa agacctgtgg
     6541 ccataaaaaa gaggggtctt tcccgcacat tttttttcag tgtagccgaa gtggtcatca
     6601 taatcgccac tgctagtaaa taaatggtcg tcatccattg caagctattt aagggcaact
     6661 gatagactga catcagtgtc gggaaggtga cattcatact ggtttcagac aaaattcctg
     6721 aaaaaccaat gagtgctatg gcgataatcg ccataattgt ttgttttgaa acggtttgtt
     6781 gatgatgtga catgataact cctttatcta atcgattgtt attgtatcat ataatcttaa
     6841 aattgtcaga aactcttttt tgctaaatca atagatacga ataacaatag ttgctctttc
     6901 agagaatttt ttgaaaacgc ttgacagaat cttaaagaga tgataacatt aaagcgttct
     6961 cagatgtgat tacttcaagt aagcaggcta gagacataac aatcagaata ttaaaggatg
     7021 ttatcacagt tttgtgagca gggacctatt cgaaaagtgc tttttagcga gcttttggat
     7081 aggtcatttt ttattgtgat actaaaggac taggactttt atgttgatta aacgcgtttt
     7141 aaaccataat gcggcgattt cgaccaacca tcaaggatta gatattttat tgatgggaaa
     7201 agggattgcc tttggaaaaa aagttggaga tagtattgag cttaatgcta tcgaaacatc
     7261 atttgtttta aaaaatagtg acaatatgaa tcgcttcaca gagttattta ttacggtgcc
     7321 acaagaagtt gttgcttgct cagagagaat tatcaatcta ggtaaaatta aattaggcaa
     7381 aaccttagat gaaattctct atattaattt aacggatcat attcactctg ctattgaaag
     7441 gcatgagcaa ggaatgttga ttcataaccc actacggtgg gagattcaac gctattatcc
     7501 tgatgaatat agtctagggg taaaagcctt agagctaatt gagcgtaatc taggagtcac
     7561 tctagctatt gatgaagcag cttttattgc catgcatttt gtcaatgcta gtttggacac
     7621 tccttttaag gagccacatc gcctgacgga gattgtttct tatattgagc aaaaaatcaa
     7681 aacagacttc aaaacagaat tggatgacac atccatcgat tattaccgct ttatgaccca
     7741 tattaaatta tttgctcaac gtgtgttatc agagatgtct tacgatgatg atgatgcaga
     7801 actgcttata gtggtgaaaa cgaaataccc taaagaatat cggtgcgtct tagatattag
     7861 tgaggagata aaaaagcggt acaactatca ccttaattca agcgaacttt tgtatttgac
     7921 ggttcatgtt aaacgtttag tgaaacattt aaaggagact taatatgacg tatcaagaaa
     7981 ctgccaaggc tattttagcg gcggttggtg gcaaaacaaa tattcaacgt gtgacacact
     8041 gtgtaacacg tctacgctta gtactgaaaa atgatgagaa agttaaagat cagcaagtca
     8101 aatccatttc aaatgtgatt ggcgtgatgc gcaaaaacgg ccagtaccaa attatcttgg
     8161 gcaatgatgt caataattat taccaagcct ttttaagcct agggcatttt gacaatcaag
     8221 atgaagatca ttcttcaaaa gcgaaaggaa gtatccttga gcgcttgatt gaaaccattg
     8281 ctggtgtgat tacaccgctg attccagccc ttcttggtgg tggaatgctc aaagtggtgg
     8341 ggatcttgct tcctatgctt ggcttagcga gtgcggactc tcaaaccgtt gcctttatta
     8401 acttctttgg cgatgcggct tattatttta tgcctgtcat gattgcctat tcagcagcgg
     8461 cgcgctttaa ggtgacacct gttttagcag cgacgatcgc agggatttta ttgcatcctg
     8521 cctttgttgc aatggtagca gaaggtaaac cattggcttt atttggagca ccagtcactc
     8581 ctgctagcta tggctcatct gttattccca ttttgatgat ggtttacttg atgcaataca
     8641 ttgaaaaatg ggtcaatcgc ttggtgccaa gcgtgatgaa aagcttctta cagccaacct
     8701 taatcatttt gatttctggc tttttggctt tagtggttgt aggtcctctt ggtgtgatta
     8761 ttggtcaagg gttatctaat accatgctcg ctatttatca tgtggctccg tggctagcac
     8821 ttgccatttt gggagcgatt atgccgcttg ttgtcatgac ggggatgcac tgggcttttg
     8881 caccaatctt tttggccgct tcggtcgcaa caccagatgt cttgatttta ccagcaatgt
     8941 tggcttctaa tttggctcaa ggagccgcat ctttggctgt tgcctttaaa acaaagcaga
     9001 aacaaacacg tcaagttgcc cttgccgcgg ggatttcagc tttgctggca ggtatcacgg
     9061 aaccggcact ttatggggtc acactgaaat ttaaaaagcc actctatgca gccatgattt
     9121 caggtggtct ggttggagcc tttattggtt ttgtcaatat tgcttcttat acctttgtcg
     9181 taccttctat tattggttta ccacagtaca tcaacccatc aggcggagct aattttacaa
     9241 atgctttgat tgccggagct gcgacgattg tgttagcctt cagtttgact tggttcatgg
     9301 gaattgatga agaatcccca gagcaagtga gtgttgcagc agatatgtca caagttaaga
     9361 gcggcttgtc aaccaaacaa acgttgtatg ctccaatgac aggtgagatg ctttttctat
     9421 cagaagttcc tgatgaaacc ttttcttcca agttattagg agaaggattt gccatattac
     9481 caagcgaagg tgaggtctat gccccctttg atggtgaagt catcactttc tttccaacca
     9541 agcatgctgt tgccttaaaa aacacacgtg gtgtggaagt cttgattcat gtcggtattg
     9601 atacggttga gttaaaaggg caaggtttcg agcagttagt gtctgttggc gatttcgtga
     9661 aacgaggcca ggcacttcta aaaatggata ttgatttcat tacttcaaaa ggctattcac
     9721 tcatcagtcc tgtggtcgtg accaattcgg ctgagcagtt ggagattatt attcaagatg
     9781 acaaaaaaat agtgactaaa gaggacgctt tgttagtcat tttataacct aaaaggagag
     9841 ggagaatggg aatttttcca aaagatttct tatggggcgg cgcagtagct gccaaccaag
     9901 tggaaggagc atttgaggca gacgcaaaag gtttgtccgt acaagatgtt ctaccaaatg
     9961 gtggactagg agaatggaca gatagtccta ccagcgataa tttaacttta gaggcgattg
    10021 atttttacca ccgctacaaa gaagacattg ctctttttgc cgagatgggc tttaaagtct
    10081 ttcgaacgtc tattgcttgg tctcgtattt ttccaaatgg tgacgatgac cagcccaatg
    10141 aagcaggttt gcaattttat gatgacttat tcgatgaatt gttgaactat ggtatcgaac
    10201 ccttagtgac cttgtcgcat tatgagacac ctctgcattt ggccaaagcc tataatggtt
    10261 ggacagatag acgtctgatt ggcttttttg agcgctttgc tcagacagtc atggaacgct
    10321 ataaggacaa agtcaaatac tggttgacct tcaacgaagt caactcgatt ttacacatgc
    10381 cctttaccag tggtggcatc atgacagaaa aagaaaagct gagcctgcaa gatttatacc
    10441 aagctattca tcatgaattg gtagcatctg ctagtgtcac aaagctagcg catgaaatca
    10501 atcctgatgt taaagttggg tgtatgattt tggcgatgcc tgcttaccca atgacctctg
    10561 atcctcgtga tatacttgct gcgcatgcat ttgaaaatct taatctctta ttttcagata
    10621 ttcatgtgag aggcaagtat ccttcatata ttaagtcgta ttttaaagaa aatgggattg
    10681 agattgtttt tgaagacgga gacaaggaat tattagcgga acatacggtt gattttctat
    10741 cctttagcta ttacatgagt gtcacacaag cccacaatcc tgaagcttac acatctggtc
    10801 agggaaatat tctaggtggt ttgtccaatc catatttaga atcttcggag tggggttggc
    10861 agatagaccc aattggtctg cgattggtct tgaatcagta ctatgaccgc taccagattc
    10921 ctttgtttat tgttgaaaat ggcttaggtg caaaggatca gctggtgcaa accgcagatg
    10981 gcagcatgac ggttcacgat gattatcgca ttgactacat gagtcagcat ctcgtacaag
    11041 ttgccaaagc gattgaagat ggcgttgagg tcatgggtta cactgcttgg ggctgtattg
    11101 attgtgtctc tatgtcaaca gcccaattaa gtaagcgcta cggttttatt tatgttgacc
    11161 gaaatgatga tggtactggt caactcacac gctataagaa aaaatcattt gattggtacc
    11221 gacaagtcat tcaaaccaat ggacgttacc ttgaggataa tttttcctaa ataggtcttg
    11281 tttaagatgg tgactctgtt cctatgctaa ctgataaaac gcaaaaagca tttgttttga
    11341 taaaaaacaa gtgctttttt attagtcttt aaaaaacgcg gtaaacataa ggattattag
    11401 gttctttggt ttgatgaacc tcagcaagtt gaaggacagg tagatgttgt tctaagtttt
    11461 tatcatattc catatgaagg cgacctttaa ctgttagcca tgtattgtta gggtagctgg
    11521 tttgattacc tgtggtcaaa agtccgtaca ctcctgagtc ggcaatacag tgaataatgc
    11581 cgaagcggaa aaggaattgg tagttgtcat ggcccggttc gttataaaca aagccagtgt
    11641 attggatgtc acgatcaaga aactcatctg ggtagagata gatcaattcc atcacttcca
    11701 tataattttc agtggtaatg gtgaccggtt tcttgccttt atatttatgg agttcttgac
    11761 gcatctcctt ttggtaggca gactttgtaa agtaaaggct ggtatcaggt ttgaggtatt
    11821 gaatggtggt accgtcatca ctgactcccg ttttagaggc tcccgcagcc aatgggaaag
    11881 tatagccttt agcagaaacc gtggtggaat ctagggttac tgttggcaca aggagaccaa
    11941 tcaaaacagg aaagaccaag ataaaaggac tggtcagcct tgcgattttt cctgttaaat
    12001 ggctatgtac cttaatgttt ttcatccagg tatagagttg aacaagagct aaaatgaaag
    12061 ataaaaccat ggaaatataa gcaaggtaag aataccgaac attgatgtac tgatccagtt
    12121 tgccagacag ctggagatac atggttagtt caaaatagcc agataaaatt aaaaagcgaa
    12181 tcattagagc acccccacaa gtaaacaata aacggcaatc ataagaacgg atacgccgat
    12241 aaattggaca atgaagcgtc ctttaaaagc ctttaccatc atcataaggt ttttaatatc
    12301 caccatagga ccaattaaaa gaaaggcaag aactggagcc actccaaagg tggataataa
    12361 ggaagctccg ataaaggcat cggcttcact acataatgat agaatgaaag ccatcagcat
    12421 catgatcaaa atggctgtca agggattgtg cccaatcgtt gtcaacacac gagttgggac
    12481 atatatttgc atggcagagg caatcaaggt tccaaatacg agatacctac cggtgtcgaa
    12541 gaattcatca atagcatgta ccagtgctag ataaatgcgt tttggcaagc tttcatgaga
    12601 ataatcatga aaatgcacgg gttgagcatt atccttgaga atgttatcat ctacaataaa
    12661 agctaacatt accccaagtg taatggctac tagtgcagca ccgaccaagc gaagtatcag
    12721 aaagcgcaaa gaattcccaa aagcagaata agtagcgaac aaaacaatag gattaataat
    12781 aggcgctgtt gccagaaaag ggacggccgt ataactaggc accttttttt ccaaaaagcg
    12841 gttgatgatg ggaataatcc cgcattcaca agaaggaaaa acaaatccaa caaaggttcc
    12901 aaacagaatg cgcaagcatt tttgcttagg taggtatttt tgaaccaatt ctggcgttac
    12961 aaagacttca atacaaccag ataaaatagt tcctaggagc acaaaaggaa gagcttcaat
    13021 gataatggac ataaaaatcg caaaccattg caagacactg ggaggaaggt tggaaaagag
    13081 tgacattcac catatccttt ctagatgata aaagaggagg ctgaaacacg aatgttcagc
    13141 ctcgactgat acgttttgtt agcctgatcg ctaaaaatct ctcaactcag tttaaacgaa
    13201 tggacgatat gttaagagaa tgagacaaac tagctatggt ttgcttggtt ttgtttgttc
    13261 tgtcgttttt tctttggtcg ctagtttacc atcagtattg acttccaaag gcctatcaaa
    13321 actagggatt gatgcaaagc ctgctagcat tttttcaagt tcatcatttt ttttatgggt
    13381 aattgccata tctatgacct tctttcattc aagataagtc tattatacta tcatttgaga
    13441 acaatgccaa aacggaagtg ttgtttactt ctttcttttt taaaattagg tattgttatt
    13501 tagggtttcg tcagttattt actaaaagta gctttttttg attctttacg caactttata
    13561 ttaggacaat gtttcgctat cattttttgc attaatatgg tttgtgttat aatagcaata
    13621 ttatggaaaa taacaataat cacaacattg cagaggcttt gtcagttagt ctccatcaaa
    13681 ttgaacaggt gcttgcccta acagcacaag gcaataccat tccctttatt gcacgttacc
    13741 gtaaagaagt aacaggaaat ctagatgaag tggtgattaa gtccattatt gatatggata
    13801 agtctctcac taccctgaat gagcgcaaag caaccattct cgctaaaatt gaggaacagg
    13861 gaaaactgac agatcagtta cgaacgagta ttgaagcaac cgaaaaactc gctgatttgg
    13921 aagagttgta cctgccttat aaggaaaaac gccgtacaaa agcgacgatt gcgcgtgaag
    13981 caggcttgtt cccattagcg cgtttgattt tacaaaatgc gcaaaacctt gaaacagcag
    14041 cagaaccctt tgtcacagaa ggatttgcca gcccacaaga agctctagca ggagctgtgg
    14101 acatccttgt ggaggccatg tcagaagatg ctaaactacg ctcttggacc tacaatgaga
    14161 tttggcagta cagccgctta gtatcaacgc ttaaagatga gcagttggat gagaaaaagg
    14221 ttttccaaat ctattacaat ttttctgacc aagtgtctaa catgcaagga tatcgtactt
    14281 tagcccttaa ccgtggcgaa aagttaggca tcttaaaagt gtcttttgag cataacttgg
    14341 agaaaatgca acgctttttc agtgtgcggt tcaaagaaac caacccttat attgaagagg
    14401 tcatcaatca gaccatcaaa aagaaaattg ttccagctat ggaaagacgg gttcgttcag
    14461 aactcagtga tgccgcagaa gatggggcta tccacctctt ttctgaaaat ctccgtcatc
    14521 ttttgttagt gtctccttta aaagggaaaa tggttcttgg gtttgaccct gcctttcgaa
    14581 caggtgcaaa attggctatc gttgatcaga ctggaaaatt gttgactact caggttattt
    14641 atcccgtagc accagctagc caaacgaaga ttcaagcagc taaagaaaca ctgactcagc
    14701 tcattgagac ttaccagatt gatattattg ccattggaaa cggaacagcc agtcgggaaa
    14761 gtgaagcctt tgtagcagac gttttgaaag atttcccaaa tacgtcctat gtcatcgtta
    14821 acgaaagcgg tgcttctgtt tattcagcgt cagagttagc ccgccatgag tttccagacc
    14881 tcacagtgga gaagcgctct gctatttcta ttgctcgtcg cttgcaagac ccactcgcag
    14941 aattggttaa aattgatcct aaatctattg gcgtcggtca gtaccagcac gatgtgagtc
    15001 agaaaaagtt gagtgagaat cttggttttg ttgtggatac tgtggttaac caagttggtg
    15061 ttaatgtgaa cacggctagt ccatctttat tagcacatgt gtcaggtttg aacaaaacga
    15121 tttcagaaaa tattgtcaaa taccgcgaag aaaatggagc actgacatca cgcgctgaca
    15181 tcaaaaaagt gccgcgttta ggagctaagg cgtttgagca agcagcaggt tttctcagaa
    15241 ttccaggagc caaaaatatt ttagacaaca caggagttca ccccgagtcc tacccagcag
    15301 tcaaagaact ctttaaggta cttggtattc aggacttgga cgacgctgcc aaggcaactt
    15361 tagcagcagt tcaagttccc caaatggcag aaacattggc tattgggcag gaaaccctta
    15421 aagatatcat tgctgatctc cttaaaccag gtcgtgacct tcgtgatgac tttgaagcac
    15481 caatcttacg tcaagatatc cttgatttga aagatttgga aattggccag aagcttgaag
    15541 gaactgtgag aaacgtcgtt gactttggtg cttttgtaga cattggtgtt catgaagatg
    15601 ggcttattca catttctgaa atgagcaaaa cctttgtcaa ccaccctagt caagttgttt
    15661 cagtgggtga cttggtaact gtttgggtct ctaagattga cttggaccgc cacaaggtca
    15721 atctcagcct attgccaccg cgtgacactc actaattatg tccaagaagt ttccctggct
    15781 gattttggaa aaccatttca ccacaaggct tattggaaca agcgcctaaa aacaactggc
    15841 ggtcgctttt ttccaaaaga tggtcatttg gattttaacc ctaggatgct tgaggagcac
    15901 ggtgagctta tttttcggaa aattgttcgc catgagctct gccattacca tctttatttt
    15961 gaaggaagag ggtaccatca taaagatagg gatttcaaag atctgctagc tcaagtaaac
    16021 gggcttcgtt atgtcccaac tagctctaaa tcaaagacta atcatcatta tagttgccaa
    16081 acctgtgggc aggtttatca acgaaaaagg cgtatcaatc tggctaaata tgtttgtgga
    16141 aattgccacg ggaaacttat ggaaaaaaat cagtcgtaag actggtttta ttttgcgaaa
    16201 aacaatgtaa actagtatca tagaatgagg aggtaaagaa acgtggaaac taaattctat
    16261 aaacagcgaa aaaatcgctt ggttgcaggt gtcattgctg gtttagcaga taagtatggt
    16321 tgggatcttg ctctagcgcg tgtcttagcg gctcttctta tttacggaac tggttttgga
    16381 gttttgcttt atattttgtt ggccatcttc ttaccttaca aggaagactt gcttgaagaa
    16441 cgttatggcc gaggtcctcg tcgtcgtaaa gacgctgatg tcttaaatga agaagaagat
    16501 ggctggttct ggtaaacaat cagttaaaat gataattcat cacgtaagag caaccatgtg
    16561 attaactgat gataataaaa tgttttgata tgtaagtaag cgaggggtct tatcagctat
    16621 gatgaggctt tttgcttttt gacttagtaa gatgatacct ttttaaatac cataataagc
    16681 actaattgct caatgatact agtaaagtat ttgtttgcca ttgtcttatt tttgactcat
    16741 atctctaaat gataaaacct agtttttaat agagctaaga ccatttttgg tgtcgttgtt
    16801 ctcatttagt agtgaaaaaa tgagtatttg caagggtcta acggtcgatt tgtgttataa
    16861 tattgaaaaa ggagaaatta tgaccgttac tgtaaagatg ctagtgcaaa aagttaaact
    16921 tgatgtggtt tatgctacag ataacttgct gtcaaaagag ataacaacat cagatatttc
    16981 acgtccaggt cttgaaatga ctggttattt tgattattat gctccagaaa ggttgcagct
    17041 gtttggaatg aaagagtggt cttatcttac acagatgact tctcacaacc gttattctgt
    17101 tttaaaagag atgttcaaaa aggacactcc tgcagtggtt gtctctagaa atttagccat
    17161 tccaaaagaa atggttcaag ctgcaaaaga agaaggtatt tcacttttaa gtagccgtgt
    17221 ttcaactagc cgcttagctg gagaaatgtc ttattttctt gacgcttctt tagctgaacg
    17281 tactagtgtt cacggcgttt taatggatat ttatggtatg ggggttctta tccaagggga
    17341 ttctggaatt ggtaaaagtg agactggttt agaattagtt aagcgagggc atcgtttggt
    17401 tgcagatgac cgtgtggatg tctatgccaa agatgaagaa accctttggg gagagccagc
    17461 agagatcttg cgccacttgc ttgaaattcg tggggtaggt attattgatg tgatgtcgct
    17521 ttatggagct agtgctgtga aggactcatc acaagttcaa ttggccattt accttgaaaa
    17581 ctttgaggct ggtaaagttt ttgatcgtct cggaaacggt aatgaggaga taactttctc
    17641 aggagtgaga atccctcgta ttcgtattcc ggtaaaaaca ggtcgaaatg tttcagtggt
    17701 tattgaagcc gctgcgatga atcatcgggc taaagaaatg ggctttgatg ctacaaaaac
    17761 ttttgaagat cgtttaactc aactaatcac caaaaatgag gtgagtcaat gatcaatccc
    17821 attgccttaa aatgtggtcc actagccatc cactggtatg ctctatgtat cttgtcagga
    17881 cttgttttgg cggtttactt agcatcaaaa gaagcaccca aaaaaggaat atcatctgac
    17941 gctattttcg attttattct gattgctttt ccacttgcta ttgttggtgc aagaatttac
    18001 tatgttattt ttgaatggtc ttattatgtc aagcatttag atgaaatcat agccatttgg
    18061 aatggcggta ttgctattta tggcggtctc attacaggag ctcttgtact tttggcttac
    18121 tgttataaca aggtgctcaa tcccattcat tttttagata ttgccgcacc aagtgtcatg
    18181 gtcgctcaag ctatcggtcg ctggggaaat tttatcaacc aagaagccta tggtaaagct
    18241 gtgagccagt taaattactt accaagcttt attcaaaagc aaatgtttat agagggaagt
    18301 taccgcattc cgacctttct ctatgaatca ctttggaatc ttttgggatt tgtcattatt
    18361 atgatgtggc gtcgtaagcc aaaaagtcta ttagatggag aaatctttgc attttactta
    18421 atttggtatg gtagtggaag actagtcatt gaaggtatgc gaacagatag tcttatgttt
    18481 ttaggtattc gcatctccca atatgtgtct gccttattaa ttattattgg cttaatcttt
    18541 gttataaaaa gacgtcgtca aaaaggaatt tcttattatc aagaataagg atagaaaaag
    18601 gagaaactta tggacttagt tggaatctca ctgatgatta ttgcactcgg atttgttgct
    18661 ttggtgattt tcttgattat tgtattaaag aaagtctcag aaacaattga tgaagccaaa
    18721 aaaacgattt ctgtattgac aagtgatgtt aatgtgacac ttcatcaaac taatgatatt
    18781 ttagcaaaag ctaatatcct tgttgaagat gttaatggta aagtagcaac catcgatcca
    18841 ctgtttgttg ctattgctga tttgtcagaa agtctttcag atttaaatag tcaagcaagg
    18901 cattttgggc aaaaagcaac taatgctaca ggtaatgttt caaaagctgg aaaattagca
    18961 ttagttggta aagtagcctc aaaagtattt ggaaaaaaag gagaaaagca tgaataaaag
    19021 tttcaaaaac ttagtgattg gagctgtttc aggagtagcg gcagcttact ttttatcaac
    19081 agaaaaaggc aaggctttga aaaatcgtgc tgaaaaagct tatcaagcct acaaagaaag
    19141 tccagatgat taccaccaat ttgctaaaga aaaaggtagt gaatacagcc atttagcacg
    19201 tgatactttc tatgatgtga aagataagtt ggcttctggt gacttaacta aagaggacat
    19261 gttagacttg ctcaaagata aaacaacagc tttcgtgcaa aaaacaaaag aaacccttgc
    19321 cgaagttgaa gcgaaagaaa agcaagatga tgttatcatt gatttaaatg aagaagacat
    19381 catcattgac tatactgaac aagatgagcc tgttagtgat acactagata aacactaact
    19441 aaaacgacct aaaacataga agaagaaaag attgaaggat ctgatcacta gtcccgatgg
    19501 ttagttttag gctaactgaa gaaggggggt attgtggctt caatcttttt ttgtttttga
    19561 cacaaaaaaa agagtcacgt aaaacggact ctggaagtta catcagtaat gatttgataa
    19621 ccttggctaa taaggaagta atgcctaagc tgatgatgct tgcaaataag aaagcaaaca
    19681 ttggcgctaa attcattacg agcaggacaa atgcaaataa aacggtaata aggctgaaac
    19741 tagcgatacg agcaaagaag actaattctt ttagctttgc actttggcca ttgaactctt
    19801 gaaaatctcg aaatttactt tgagattctt ggtaagaatc atcctgttca accgaattat
    19861 gcttctttaa cggtgcaggc atagtcctct cctttcctta acatactaag taattctatc
    19921 ataaaagtct cttcttgtca tcttttttta aaagatttga aaagatttac taatgacaag
    19981 tgacttatca tgctataaca gtaaggggac tagcttaacg tttgggcttt tgcaagaaat
    20041 atgcttaagg tcttaaaatt tttggtataa taaagcttat atggaaaaaa taattattac
    20101 agccacagct gaaagcattg agcaagtcaa agctttatta gctgctggtg ttgatcgtat
    20161 ttatgttggt gaagccaatt acgggttacg tcttccccat aacttttctt atgatgaatt
    20221 gagacaaatt gccaaactag tgcatgatgc aggcaaagag ttgacagtag cttgcaatgc
    20281 tttgatgcat caagatatga tggatcagat caaacccttt ttagacttaa tgatagagat
    20341 tgctgttgat tatcttgtgg ttggtgatgc aggtgtgttt tatgtcaata aaagggatgg
    20401 ctataacttt aaactgattt atgatacctc tgtttttgtg acttctagcc gtcaggtaaa
    20461 tttctggggg caacatggtg ctgttgaaag tgttttagca agggaaatcc catcagcaga
    20521 gttattcacc ttagctgaaa atttggaatt tccagcagag gtgctagttt acggcgcctc
    20581 ggtgattcat cactcgaaac gtcccttgct agaaaattat taccatttta caaagattga
    20641 tgatgaggtt agtcgtgaac gaggactttt cttggcagaa ccaggagatg ctagcagcca
    20701 ttactccatt tatgaagaca atcatggcac tcatattttc attaataacg atatcgacat
    20761 gatgtctaaa ttgggtgagc tgtatgcgca tggcttaact cattggaagc tagatggcat
    20821 ttactgtcca ggtgatgatt ttgtcgctat tactaaattg tttattcagg ctaaaacctt
    20881 attagaagct gggcaattta cccaagaaga agctgaaaag cttgatcaag cagtgcatgc
    20941 ccatcatcca gctggccgtg gattagatac tggcttttat gagttcgatc caaaaaccgt
    21001 taaataagta atccttgtct tgagtgagtt tactatttca gggcaagaca gggttatgac
    21061 accattacac aggtaatcta cctatcttga aaatagaaag aatatcacat gtcacatatg
    21121 aaaaaacgtc ccgaggtctt atcacctgct ggaacacttg aaaaattaaa agttgcgatt
    21181 gactatggcg cagatgctgt ttttgttgga gggcaggcct atggcctaag aagccgcgct
    21241 ggtaacttct ctatggaaga attgcaagaa ggcattgatt atgcacatgc gcgtggagct
    21301 aaggtctatg ttgctgctaa catggttacc cacgaaggga acgaaattgg tgcgggcgag
    21361 tggtttcgtc aactgcgtga tatggggctt gatgcggtca ttgtttcaga tccagccttg
    21421 attgttattt gttcaacaga agccccaggt ttggaaattc atttgtcaac gcaagcttca
    21481 tctaccaatt acgagacctt tgaattttgg aaagccatgg gcttgacacg tgttgtttta
    21541 gctcgcgagg ttaatatggc cgagttagca gaaatccgta agcggacaga tgtggaaatt
    21601 gaagcctttg tccatggagc catgtgtatt tcttattcag gccgctgtgt tttgtcaaac
    21661 cacatgagtc accgtgatgc caataggggc ggctgctcac agtcttgccg ctggaagtat
    21721 gatttgtatg acatgccatt tggaggagag cgccgctcct taaaagggga aattccagaa
    21781 gactattcta tgtcctctgt tgacatgtgt atgattgacc atattcctga cctgattgaa
    21841 aatggggttg atagcttaaa aattgaaggc cgaatgaaat ctatccacta cgtctcaacc
    21901 gtaaccaact gttacaaggc ggctgtagat gcttacatgg aaagcccaga agctttttat
    21961 gctatcaaag aggaattgat tgacgagttg tggaaggttg cccagcgcga gttggctaca
    22021 ggtttttatt atggtatccc aactgaaaat gaacaattat ttggtgctcg ccgcaaaatt
    22081 ccacaatata aatttgtcgg agaagtagtt gcctttgact cagctagcat gacagcgacc
    22141 attcgtcagc gtaatgtcat catggaaggc gatcggattg aatgttatgg accaggtttc
    22201 cgtcattttg aaacggttgt taaggactta catgatgcgg atggccaaaa gattgaccgt
    22261 gccccaaatc caatggaact cttaaccatc tctttaccga gagaagttaa gccaggggat
    22321 atgattaggg cttgcaagga aggccttgtc aacctctatc aaaaagatgg caccagtaaa
    22381 actgttagaa catagtaaca agaaatacta gaggccctct ttttccaaat ggtatacgac
    22441 catagttggg aaaagagagc ctgttttttt tgaaaattgg atatcacaat agcttatttt
    22501 ctcaaaactt gatagaataa taggagaatg agtcataaaa aaagtgaaaa aaagatgatt
    22561 ttttccactt taatttcgaa tagtatagat gaggaggaaa actaatggca gaatttacat
    22621 tcaacattga ggaacattta ctcaccttat cagaaaatga taaaggttgg acgaaagaat
    22681 tgaaccgcgt cagctttaat ggcgcagaag cgaaatggga tatccgcacg tggagtccag
    22741 accatacgaa aatggggaaa ggcattactc ttactaatga agaatttaaa accatcttag
    22801 acgcttttcg taagtaatgt taccttaatc gaaacataat ccctcgttcg cccttgtcct
    22861 cttaacctgg tgatgacatc gctaggttaa gaggacaggg gcgaatagaa ggattatttt
    22921 tttgaattga ggcgatcagc aaggccgaca agtaccaaaa ttaagcctag cccaagaaaa
    22981 atccatttgg cagctaaaag gccataaagc aagaagatag tagcactaat gtaggagata
    23041 tattctttgg tcatcttgtt aagtcacttt ctataagcta ctgttaataa accagtcttc
    23101 atggtaatga agactggtta gagataataa aacgttatgt tatattgtca cttatcggtt
    23161 ttaagatatg aatacaaatc ttaaaaaagt taaggtttca tcgttgggaa taaaagcaca
    23221 tctctgattg ttgttgtgtt tgttaataac atgcaaagac gatcaatccc gattccgagt
    23281 ccaccggttg gcggcatacc atattcaaga gcttcaacga agtcgtagtc aatgccagtt
    23341 gcttcgtcat caccaagttc tttagcttgt gcttgtgctt caaaacgaga taattggtca
    23401 atgggatcat ttaactctgt gaaagcattg gcgtattctt tggtcatgat gaaaagttcg
    23461 aagcggtcgg tgaagcgggt atcttctgga ttcttcttag ccaaaggtga tacttcaaca
    23521 ggatgaccaa agacaaaggt tggttggacc aaggtttctt caacgaattc ttcgaagaaa
    23581 gcattgatga tatgtccgac acttgtgaag tgtttttcga gtgggacctg tttttctttg
    23641 gcaagtgcaa tagcttcttc gacagtcatt tcaggccaga aatcaacacc tgtcacttcc
    23701 ttgatagcat ctaccatgtg gacacgtttg aaaggttcat tgatacggat ttcagttcct
    23761 tgataatcaa taggcccatc acctttaaca gctttagcag catgttggat aatgccttct
    23821 gtcaggttca tgatatcaag atagtctgca taggcttggt aaacctcaat agaagtgaac
    23881 tcaggattat gagtagcatc cattccctcg ttacggaaga tacgaccaat ttcatagaca
    23941 cgttccatac caccaacaat caaacgtttc aaatgaagtt ctgttgcgat acgaagcacc
    24001 atatcaatgt tttgagcatt atggtgagta acgaaaggac gagcagcagc accaccagcc
    24061 tcgttatgaa gcacaggcgt ttctacttct aggaaatcaa gaccgtctaa ataacgacgg
    24121 atttctgaaa tcattttgga acgggtcacg aagcggtcaa agctttcacg atttgaaatt
    24181 aaatcaaggt gacgcttacg gtaaatggtt tcaatatcag ttaatccgtg gaatttttca
    24241 ggaagaggac gaagtgattt agaaaggtga gtcaacttgg ttgccttgat tgacaactcg
    24301 cccatgtctg tacgcatgac ttcgccttca acgccgataa agtcgcctaa atcggctttt
    24361 ttgaagattt catagttgtc ttcgccaaca gagtccttgc gaacataaag ttggatttgg
    24421 ccttcacggt cttgaagatg agcaaaacca accttacctt tgccacgttt tgtcatcaag
    24481 cggccagcaa caatagctgt ttcgtttaac tcatgtaatt cttcttttgt tttatcagca
    24541 tacttttctt ttaattcagc agaattggct gtgcggtcaa agcgtttacc gaaaggatca
    24601 attccttgtt cagccagagc catcatcttt tcacgacgga caatctgttg gtcatttaat
    24661 tcttcgatgt gttgattaga catattttcc tccaatacga tttcctttcc attgtatcat
    24721 aaaagaggct agaatacacc tatatttcgt tggaaaatgc gactgcagcg gggcactttt
    24781 ttgaaaataa aattgaaaag cctaatggaa aatagtataa tgattgggat attaaactag
    24841 aaaagaaaag agactcatga ttactgctat tgtttttgat gtggatgaca caatttacga
    24901 ccagcaagcg ccttatcgaa tcgccatgga aaagtgcttc ccagattttg atatgtcagt
    24961 aatgaatcaa gcttatattc gctttcgtca ttattcagat gttggattcc caagagtaat
    25021 ggcaggggag tggaccacag aatatttccg attctggcgt tgcaaggaaa cactgcttga
    25081 gtttggctat cgagaaatcg acgaagctgc gggagtacat tttcaagaag tctatgagca
    25141 cgaacttgag aacattacca tgttagatga gatgcgcatg acccttgact ttttaaaatc
    25201 aaagaatgtt cctatgggga ttattaccaa tgggccaaca gaacatcagc tgaaaaaagt
    25261 tcgtaaatta gggctttatg attatattga tcctaagcat gtcattgtga gccaagcaac
    25321 aggttttcaa aaaccagaaa aagaaatttt taacctggca gcagaacaat ttgacatgaa
    25381 tccacaaacc acactatatg ttggggactc ttatgacaat gatatcatgg gtgcctttaa
    25441 tggtggttgg cattccatgt ggtttaatca tagaggtcgt cagctaaaac caggaactaa
    25501 gcctgtttat gatgtggcta ttgataactt tgaacagctc tttggtgccg ttaaagtact
    25561 ctttgattta cctgataata aatttatttt tgatattaat gacaagaaaa atccgatttt
    25621 acagatggga atcaataatg gcttgatgat ggcagcagag cgtttacttg aaagtaacat
    25681 gagcattgac aaagtggtta tcttgcttcg attaaccaag cagcaagaaa aagtgctacg
    25741 tttaaaatat gccaaataga tgaaaccata atagcccact agataataat aaaaaaagct
    25801 agcaaagtga gtgtcttgtc agtaagaaat caatgtgtta gcttttttta ttattgtcta
    25861 gtggttttac acctctataa aagatttatc attccaaatt aaacagtcat aagttaaata
    25921 atccttagtt tcaagaatag taagactggt attatctaag ccacctttag cgagtaaaag
    25981 agcaggttca aaacctaata atgagcgaat agttgctgtt aagttagcgc cgtgtcctac
    26041 aataagaacg ttttgataat gtttatcttt aaacgattgg ataagatggc agacacgttg
    26101 agtcgtttgg taaattgatt cagcctcgaa ctgatctggt ttgaactggg ctagattctc
    26161 ccgaaaagcc agcatttgtt ggggatatat agctgccatt gttgctattt tagccccttc
    26221 caatttacct aatctccact cccgaagctg gtcggtgtgg taaagttttg gctgttggtc
    26281 aaaagcatct aagataatag ctgctgttgc catagctctt tgaaggtcgc tagtataaac
    26341 ggcatcaaaa tccaccttag ctaactcttt gccaagtaag tgaatttcgt cttttgcctc
    26401 ttcaagtaat ggcgaatcgc ccccagcgcc ttgaaagcga ccttctaaat tccaaagggt
    26461 tttcccatgt cttacaaaat ataatttcat ttaagcctcc ttttagagat aactgatgcg
    26521 agcgctaaaa ataaaaaagt aaccagaaaa agtttaggca taaccaagca aaaataatac
    26581 ctatagtgaa aagataaagt aaaaaagcgt agtataagtg tataatagct aggttctgat
    26641 taaagtgtaa agcccatatt agatttaaga caaatcgacc tgaatttgtc gtgatatcaa
    26701 gcctttacaa aagaaaaaga ttaacaggat caatgttaaa gcagttgaga tcaaaataat
    26761 actaaacaaa gactagctta atcaaggcga aaaacttaaa aaagaaacca gataaagttg
    26821 gcagtagtgt tcattttctc ttctttaaat cagcaaagct tgcgccaacg aaatcagcta
    26881 gagcctggct agaaatcttt atagaacgcc caacttcacc agctgaaacg attatttgac
    26941 ctttttccag tgcagtttgg tcaataaaaa taggatatga atgtttttga cgaatcccaa
    27001 caggattatt ggcaccgtga atatagcctg ttgtcttttg caagtctttt tgaggaacca
    27061 tggacacttt tttgttacct gatacttttg ccaattgttt ttctgataag tgttctgtta
    27121 atggaataat gccaattaga ggacctgttt gatcaccagt cagagcaagt gtcttgtaaa
    27181 tatctgatgg ttgaagatca tctggaaagt cgccttctaa agcattaagc ttgagtcctt
    27241 gatgagcaat attagcctta tcgaggattt gttctactaa ggtttttttg agttttgttt
    27301 ttttagccat atcaagtcct tacctttatc taaacataaa aaaatggtat aacaacatta
    27361 ttataccatt tttttaatgt aagattcaaa tgactaaaaa ttagtctttg ggatcaaata
    27421 attgaggatg aattggtaaa gcaatcatta acgctaaaca tgttattaaa aaagttagga
    27481 caccagctgt accattaacg ctaaaagaat accagacagc ggacatgcct ttaggggcgt
    27541 aactgcccca gaaaatgatt cctgcaagga agtgccacaa gtaacgtact gtaacggcta
    27601 gtaaactagc cattattgca agaaaaacgg caaaaaaggt ttgttgtctt cttaaagttt
    27661 taatcaaact atcagagaaa ctaccggcta atcccataga ggtaaaggct aagatatatt
    27721 caataaagac ttgggacatg ctgagatagt aaacttttcc aagtacaaag tgaagtagcc
    27781 cccaaatgag acccgccaac atgccgtact ttaacccacg gcgcaaagag aaaataacca
    27841 gtgcaatagc accataagaa ggactaaacc agccagcaaa atctggaata aatgaaaggg
    27901 tcatcgccaa tgcagcaaaa atagcagctt cgataaggta tttgacattg gtgtttggtg
    27961 acataaaaaa ctccttttgt gcacaaaaag agtatcgctt tccaatcatt gattattggt
    28021 cacaatccct acgcttgtac taacaagatc aggtcctcag gatttcgcaa ttgcgatctc
    28081 agccaaagca ctcctttgtg aaatattata gtgctttcat tatatgatga gataatctcc
    28141 ttgtcaagga taaggggttt taaattattg acctagagtt tttcttggta agatgtaaac
    28201 tataaaaaaa gattagccat gatgctaacc tcttaattgt tggagttgcc aaataacttc
    28261 tcaaacgttt ttttagtgtc ttttgttacg gcaatttgat ttaaatctaa agcattgtta
    28321 aaaccactga gataaccttg tgaggtatat tggtgtaaat cgtaatctaa ggttgtatta
    28381 ggagcagcct cataatagcc agaatcacta ccataagtcg gaatccatac agcatcgaaa
    28441 ccttttgttg aaatatcttg ttctgccata aaataagttc caatatagag accaacattt
    28501 tcagcaccaa gttttttaag ctcttctcgg aaggctgtta cgcctttatt catatctttc
    28561 atagtggctt cttcgacatc aatccagtag taagttggat tatagggagc agcgtttttg
    28621 tagaaagctc gggcctcttc tttcatctct ttagtactac gaccaagtgc gtaactgtaa
    28681 acagctacag gaacatttcg tttttgaaat tctttgatat gcgttttaaa agatttgtca
    28741 attccagttg taaaagcagc gttgttatga gctgtaattt gcgatcctcc atagacacgt
    28801 acaattgcgc cagagatgtg acgtgacaat gtgtcataat caatttcttc agggagttgc
    28861 cagccggaaa catcaacaat aggatttaaa ataaagtctt cggtttcaga agtgctagtt
    28921 tttgctttac taggattact ggttgcaata ggaatatgac tcttagcgtc ttcaacttct
    28981 ttttgtttcg catgattagc ttgtcttttt ccgataatca ataccattgc cagtaaaata
    29041 aagaagacta gcacaacaat aggttttatt tttcttctca ttaactgtta ttgtacccta
    29101 attactaaaa aaaatcaaaa tatcctaact gttttcttat attgtcttgc attttaaggt
    29161 attatctttt agaagttaca aatgagatcc tctctaacta tgtcaagttt atgtcactat
    29221 ctcttatagt tggtagctat attctttact atattagttt agtctttgac tatatcatga
    29281 cagtcagcaa gtactgtttt gttatttaat tctcatgtta ttatcatttg ataaataagt
    29341 gagcttcaaa ctggtaaacg ctctgtaaat ctgtcataat agagaaaaaa acgaggaaga
    29401 agtttatgtc agctaagctt atgagtcgca ttgctatcat gtcagcccta tgtgttgtgt
    29461 tgagaatggt ttttagcagc ctgcctaacg tgcagcctgt gacggcattt ttactcagtt
    29521 atcttcttta ctttgggtta gccgaggctg tattagttat gatgttgtgc ttatttttat
    29581 cagccttttt gctgggattt ggtccttggg tcttttggca agttacttgt tttgtgcttg
    29641 ttcttttatt atggcgtttt gccttgtacc ctctgtctca gcaattcccc aaatatcagc
    29701 ttggctgtca ggctttcttg gtcgccttat gtggcctcct ttatggggtg ttgatagata
    29761 cttgttttgc gtatttgtat agtatgcctt ggtggagcta tgttctagct gggatgcctt
    29821 ttaatatagc gcatgcttta tcaactctcg tcttttttcc agttgtgatg atgttattta
    29881 ggaggttaat cggtgaacaa aagatttaaa acaggatttc tagcgcttgt tgctatgcta
    29941 cttgttgctt gctcgcaagg aacaaaacaa acacagactc caagcgttcc aaaagcagac
    30001 catcacgttc gcttagttgt caaagaagat accaatacca ttgatgaaaa agtttctttt
    30061 ggaaaaggag atacggtgct tgagattctc aaagataatt atgaggttaa agaaaaagat
    30121 ggttttatca cagcaattga tggtatcgag caagacacga aagctaacaa atactggctt
    30181 tttaaggtca atggaaagat ggcagataag ggtgccgatc aaattaccgt taaagatggt
    30241 gatagcattg aattttatca agaagttttt aaatagtaac tataatataa aatagacttg
    30301 gcacgccaag tctattttat attaaccaag aagtgcttta aggtcttctt caattagttt
    30361 aggatctgtc tttgaggaat agcgtttaat gacttgacca ttttggtcaa tgagaaattt
    30421 agtgaaattc cattcgatgc gtttcccaag gggaccactt ttttcttctt ttaaccaggt
    30481 gaacaaagga tcagcgtctt taccattcac tttgatttta gcaaagcgag ggaaagtagt
    30541 gtgataggtc agactgcaaa agtgattaat ctcctcagca tctcctggag cttgatttaa
    30601 aaattggttg caaggaaaat ctaagacctc aaatcccttg tcatggtatg tgtcatatag
    30661 tgcttgcagg gcctggtatt gtggggttaa tccacacttg gtagctgtgt taacaatcaa
    30721 aacaaccttt tttttataag cagctaagga aacgtcattc ccgttttgag ccttaaccgt
    30781 aaaatcatag agattaggca tgtgacatct cctcggtgta attgataatt tggtcaacgg
    30841 tatcactcaa gaattgaatg gtatctcgaa gaggcttttc ggtggcaatg tctgccccaa
    30901 tgagcattgc agtatcaaga ggcgtacggc tgccacctga ttttaggaag tcaagccatt
    30961 cttttgcccc atctgggttg tttttaaggt taaggtaacc agctgttgac ataacaagcc
    31021 cagcagaata ggtataacta tataagccca tgtagtaatg cgcttggcgc atccaggtaa
    31081 gagctgcgtc atcgtcaatg tcaacggcat ctccccagaa gtctgttaag acctctttca
    31141 tcattgcatt taattggtca gctccaaagg taccgccttc ttcaatcagg gtgtaaacct
    31201 tacgttggaa agccgcttcc aataggtgag tgatgaagtt atgaaagtaa gtatctgtta
    31261 aacggtgagc aagagcaaaa cgtttttgac gagggtcatc aaactgatgt tccaagtaat
    31321 cactaagcat caattcatta aaggtcgatg gcgcttcgac gtaataagtt gacatatggg
    31381 tgttgaagta actttggtga ttatctgaaa aaatgaattg accagagtga ccgatttcat
    31441 gaataagggt ataaacatca gacattctgc cagtccaact cattaagaca tacggatgaa
    31501 ccttgtaggg atcagcggca tagccaccag aatccttatt ggcgttagca gcaaagtcaa
    31561 cccagcgttc tgtctgataa cgttcgattt ctttcgtgta ttcttgccct aatggggcta
    31621 gagatttcat cacgaggtca taggcaccat caatgctgac ctctggattg agctcattat
    31681 caatatctag tttccaatca gcaaaagtca ttttctcaag accattgact tgggcgacat
    31741 gttttaagaa tttctgagca accggtccaa attcagtcat gatgagatca atttggcggt
    31801 cgaacaagct acgatccact tcctgttcag ctaggaggta atcaaagaca gaagcgtagc
    31861 ctttcatatc cgctaacaat ttttcagatt taaccttggc taagtaagcc gctgcagctg
    31921 tattttggtg tttacggaga ccttttgaaa aagagcggaa ggctttttca cgaatctcag
    31981 cattttcgtg attttgatag aaattttcgt aagaaacaaa gctattttta taggttttac
    32041 catccacctc aaaatcctcc atgtcaaagt caccagcccg cattttggtg taaatatcat
    32101 agggcgcatt gataacctcg cgtaagttag ccaaagcttt ttctacatca ggacttaaga
    32161 ggtgttcttt ttgaattttt gccatgcgaa tagctgcgct gaaataaggg ttcttttcta
    32221 atgtatctaa cacgtcaagg tcggcattgg ctaaggcggt gtcaaagaag ctaagggcaa
    32281 cgctagcttt agtcatgaaa tcatcgccag cctgagcaat ttgagcaaaa ctttcatctg
    32341 aaaagtcagt ggtttgaggc ataaaggcat aagtaccaat atgactcatt tgaatataaa
    32401 tatgctcaat ttctattaag gcctgagtga aatcgtccac tgatgttaag cggccttcat
    32461 aattgcgctt aaacaggtca atatcttgta aggttttttc aatagcgagt aaaaaatctt
    32521 ggcggtcttt gtagagcgct gttagatccc aaagttcatt ttcagggaat tctgaacgtt
    32581 ttttcaattc catagttgtc tccttaacaa tatagtcaag actagtataa cagaatttta
    32641 gtggaatatg gagactaaac ctatccaaac attctttaaa ataaagcgct tacttttgtt
    32701 aaaaaacaaa cgttatgaac atgatcactt ttatttttgg taaaaagtgg tatgatagaa
    32761 gaaactagca aagaaggaga tggtcatttg ccacttaaaa agttagagag tagtaacaat
    32821 caagctatca ttgctgaaga agttgctctt ttgaaagaaa tgttggaaaa tatcacacga
    32881 cggatgattg gagatgacgc gtttacagtt atcgagtcta ttatggtgtt atctgaaaaa
    32941 caagattaca tagaattaga aaaagttgtc gccaatattt caaatcaaga aatggaagtg
    33001 atttccaggt atttttctat tttacccttg ctaatcaata tttcagaaga tgtcgattta
    33061 gcctatgaaa ttaattatca aaataatacg gatactgatt atttaggaaa attagcccta
    33121 accatcaagg atcttgctgg aaaagataat ggtaaagaca ttttggagca ggttaatgtg
    33181 gttcctgttt taacagcaca tccgacgcaa gtacaacgta aaacgattct ggaattgaca
    33241 actcacattc ataaacttct gcgcaaatac cgtgatgcta aggctggtgt gattaatctt
    33301 gagaaatggc gtcaggagct atatcgttat attgaaatga tcatgcagac agatattatt
    33361 cgcgagaaaa agcttcaggt gaaaaacgag ataaaaaatg tcatgcagta ttacgatggt
    33421 tctctcattc aagcagtgac caagttgaca acagagtaca aaaacttagc ccaaaaacac
    33481 ggtctagagt tagacaatcc caaacccatt accatgggga tgtggattgg tggggaccgt
    33541 gatgggaacc cctttgtcac agctgaaacc ttgtgcttgt cagctacggt tcaaagtgag
    33601 gttatcctca attactacat tgacaaatta gcagcccttt accgaacatt ttcactgtcg
    33661 tcaaccttgg tacaacccaa ctcggaagtg gaacgtttag ccagtttgtc tcaggaccaa
    33721 tcgatttacc gtggaaatga accttatcgc agagcttttc attatattca atcgcgtctt
    33781 aaacaaacac aaaatcaact aactaatcag ccagcagctc gtatgagtag tagtgtggga
    33841 ctaaacacat ctgcttggtc ctcaccagct agtttagaaa atcccatctt agcctatgac
    33901 tctcctgttg attttaaagc tgatctaaag gctattgagc agtcgctact tgataatggc
    33961 aactccgctt tgattgaggg ggatttgcga gaagtcatgc aagctgttga tatttttggc
    34021 ttcttcttag caagcattga catgaggcaa gactccagtg tccaagaagc ttgtgtggca
    34081 gagttattaa aaggagccaa tattgttgac gattatagct ctctttcaga aactgaaaaa
    34141 tgcgatgttc tcttacaaca attaatggag gagccaagaa ccttatcctc ggcggcagtt
    34201 gctaaatcag acctattaga aaaagaactg gctatctata ctacggcacg agaattgaaa
    34261 gataagcttg gagaagaggt tattaagcaa catattattt cacatacaga aagtgtctct
    34321 gatatgtttg agttggccat catgcttaaa gaagtgggat tggttgacca gcagcgggct
    34381 cgtgtgcaaa ttgttcccct ctttgaaacc attgaggatt tagataatgc ccgtgacatc
    34441 atggcagcct atttaagcca tgacattgtc aaatcttgga ttgcaacgaa tcgtaattac
    34501 caagaaatca tgttaggtta ttctgacagt aataaagatg gtggctactt ggcatcgggt
    34561 tggactcttt ataaagctca gaatgaatta acggctattg gtgaggaaca tggagtgaaa
    34621 atcaccttct tccatggtcg tggaggaaca gttggtcgag gaggtggccc ttcttatgat
    34681 gccattactt cacaaccttt tggttccatc aaagatcgga ttcgtctgac agagcaaggg
    34741 gaaattattg aaaacaaata cggcaataaa gatgtggctt attaccattt agaaatgttg
    34801 atttcagctt ctatcaatcg tatggtgact caaatgataa cagatcctaa tgagattgat
    34861 agttttcgag aaatcatgga tagcattgtt gctgatagca ataccattta tcgaaaattg
    34921 gtttttgaca acccccattt ctatgattat ttctttgagg caagcccgat taaggaagtt
    34981 tccagtctca acattgggtc aagaccagca gcccgtaaaa cgattacgga aattacaggc
    35041 ttgcgtgcca ttccttgggt cttttcatgg tcacaaaatc gcatcatgtt cccaggttgg
    35101 tatggggtag gttcagcctt taaacgttat attgaccgag cacaaggtaa ccttgaacgc
    35161 ctccaacaca tgtatcagac gtggcctttt ttccattcct tgttatcaaa tgttgatatg
    35221 gtattatcta aatcaaacat gaatattgcc tttcagtatg cacaactagc tgaaagtcag
    35281 gatgttcgag atgtttttta tgagattttg gatgagtggc aattgacaaa aaatgttatt
    35341 cttgctatcc aagaccatga tgatctattg gaggataatc cttcccttaa gcatagcttg
    35401 aagtcacgct tgccgtattt taatgtgttg aactatattc aaattgaatt gattaaacgt
    35461 tggcgtaata atcaacttga cgaaaatgat gaaaaattga tccatacaac tatcaatggc
    35521 atagcaactg gtttacgtaa ttcaggctaa tgctcttgag tattaattgg tatttataga
    35581 agttttcaag tgactaatca gaattttaaa ggttagccct tggagcttct ttttttgagg
    35641 attcttgact tttccttttg tttgatatag aaagatgtta taatgctaaa tgattatctc
    35701 aagaagtaga gggaaaacta tgaagattga taaaaggcac ctcttaaatt attctatatt
    35761 attgccttat ttaattttat cggtgatagg cttaataatg gtttactcaa ccacaagtgt
    35821 cagcttgatt caagcacatg ctaatccatt taaatcggtg attaaccagg gagttttttg
    35881 gataataagt ctagtggcta ttacatttat ctataaatta aaattaaatt ttttaacgaa
    35941 tacaagggta ttaactgtgg ttatgttagg agaggccttt cttttaatta ttgcccgctt
    36001 ctttacaact gctatcaaag gagcgcatgg ttggattgtg atcggaccag tcagctttca
    36061 gcctgctgag tatttaaaga tcattatggt atggtatttg gcattgacct ttgctaagat
    36121 ccaaaaaaac attagccttt atgattatca agcactaact cgtcgaaaat ggtggccaac
    36181 acaatggaac gatttacggg actggcgtgt ttattctttg ttgatggtac ttttggttgc
    36241 tgctcagcct gacttaggaa atgcttccat tattgtattg acagccatta ttatgttttc
    36301 tatcagtggc attggttacc gatggttttc ggctatccta gtcatgatta ccggtctatc
    36361 cacggtcttt cttgggacta ttgccgttat tggggtagaa agagttgcta agattcctgt
    36421 ttttggctat gtcgctaaac gttttagtgc cttttttaat ccttttcatg acttgaccga
    36481 ttcaggtcac caattagcca attcttacta tgctatgagt aatggtgggt ggtttggcca
    36541 agggttagga aattccattg aaaagcgtgg ctatttgccc gaagcacaga cagactttgt
    36601 cttttcagtt gtcattgaag agttaggtct aataggagca gggtttattt tggcacttgt
    36661 tttcttctta attttacgta ttatgaatgt tggtattaaa gcaaaaaatc cttttaatgc
    36721 catgatggct ttaggagtag gcggtatgat gctcatgcaa gtctttgtta atattggtgg
    36781 tatctcaggc ttaattccat ctactggtgt taccttccct tttttgtctc aaggtggaaa
    36841 tagcttactt gtcttatctg ttgcagtggg ttttgtacta aatattgatg ctagcgaaaa
    36901 gcgagatgat atatttaaag aagctgaatt atcttatcga aaggacactc gaaaagaaaa
    36961 tagtaaagtt gtaaatatca agcaatttca ataatattct aaaatctcct ttaaggagat
    37021 tttagaatat agacaaaccc agtttgatgc tcaaaaaact tgaactgctc tgctgatttc
    37081 ttatttttgc gtgtgacatg agcccaaaca gctgcttttt gctgtggcaa aaataaggtg
    37141 t
//
LOCUS       DAUDYX010000016        34014 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.016, whole genome shotgun sequence.
ACCESSION   DAUDYX010000016 DAUDYX010000000
VERSION     DAUDYX010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34014
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.016"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            216..1499
                     /locus_tag="VHD02_000748"
     CDS             216..1499
                     /locus_tag="VHD02_000748"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEQ5497377.1"
                     /translation="MSTSFENKATNRGVITFTISQDKIKPALDKAFNKIKKDLNAPGF
                     RKGHMPRPVFNQKFGEEVLYEDALNIVLPEAYEAAVTELGLDVVAQPKIDVVSMEKGK
                     EWTLSAEVVTKPEVKLGDYKNLVVEVDASKEVSDEDVDAKIERERQNLAELIIKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLGLGSGQFIPGFEDQLVGAKAGDEVEVNVT
                     FPESYQAEDLAGKAAKFMTTIHEVKTKEVPELDDELAKDIDEDVDTLEDLKVKYRKEL
                     EAAQETAYDDAVEGAAIELAVANAEIVDLPEEMIHEEVNRSVNEFMGNMQRQGISPEM
                     YFQLTGTTQEDLHNQYSAEADKRVKTNLVIEAIAKAEGFEATDSEIEQEINDLATEYN
                     MPADQVRSLLSADMLKHDIAMKKAVEVITSTASVK"
     gene            1711..2286
                     /locus_tag="VHD02_000749"
     CDS             1711..2286
                     /locus_tag="VHD02_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEQ5497378.1"
                     /translation="MKLDVFAGQEKSELSMIEVARAILEERGRDNEMYFSDLVNEIQN
                     YLGKSDAGIRHALPFFYTDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     AQKRKKKRVNAFMDGDEDAIDYRDDDPEDEDFTEESAEVEYDEEDPDDEKSEVESYDS
                     ELNEIIPEDDFEEVDINEEDEEDEEDEEPVL"
     gene            2543..4147
                     /locus_tag="VHD02_000750"
     CDS             2543..4147
                     /locus_tag="VHD02_000750"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEQ5497379.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITDALKKKIKRAASTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSENVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPVE
                     QGIKNKLAQFCDVNSEAVIESRDVEHLYQIPLNLQAQSMDQIVCDHLKLNAPQADMTE
                     WSAMVDKVMNLRKTTKIALVGKYVELPDAYLSVVEALKHSGYANDTAIDLKWVNANDV
                     TVDNAADLLGDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGICLGMQLTCVEFA
                     RHVLNMEGANSFELEPSTKYPIIDIMRDQIDIEDMGGTLRLGLYPCKLKPGSKAAMAY
                     NNQEVVQRRHRHRYEFNNKFRPEFEAAGFVFSGVSPDNRLVEIVELKEKKFFVAAQYH
                     PELQSRPNRPEELYTAFVTAAIKNSN"
     gene            4315..5241
                     /locus_tag="VHD02_000751"
     CDS             4315..5241
                     /locus_tag="VHD02_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEQ5497380.1"
                     /translation="MKTIRIAKYLGILFLLITLISVGASFYFFHVAQIREEKSFINNK
                     KRSTNNPLYPAEQSFDALPYEKRQLTNRGLKQVGWYLPAAQKTKKTAIVVHGFTNDKE
                     DMKPYAMLFHDLGYNVLMPDNEAHGESEGNLIGYGWNDRLNVMAWTDQLIKENPESQI
                     TLFGLSMGAATVMMASGERLPAQVTSLIEDCGYTSVWDELKFQAKAMYNLPAFPLLYE
                     VSALSKIRAGFSYGEASSVKQLAKNKRPTLFIHGDKDDFVPTKMVYDNYKATKGPKEI
                     LIVKGAKHAKSFETNPEQYQKKIAAFLKKVEK"
     gene            5295..5380
                     /locus_tag="VHD02_000752"
     tRNA            5295..5380
                     /locus_tag="VHD02_000752"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5329..5331,aa:Leu,seq:aag)
     gene            5586..6467
                     /locus_tag="VHD02_000753"
     CDS             5586..6467
                     /locus_tag="VHD02_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEQ5497381.1"
                     /translation="MAIVSAEKFVQAARENGYAVGGFNTNNLEWTQAILRAAEAKQAP
                     VLIQTSMGAAKYMGGYKVCQTLITNLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLAKTAEVVKIAHAKGVSVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPENWEGLALDHLEKLTAAVPGFPIVLHGGSGIPDDQI
                     KEAIRLGVAKVNVNTESQIAFSNATREFARNYEANEAEYDGKKLFDPRKFLAPGMKAV
                     QGAVEERIDVFGSANKA"
     gene            6849..7037
                     /locus_tag="VHD02_000754"
     CDS             6849..7037
                     /locus_tag="VHD02_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEQ5497382.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTILVDGKP
                     KKVWASARALKSGKVERI"
     gene            7189..7554
                     /locus_tag="VHD02_000755"
     CDS             7189..7554
                     /locus_tag="VHD02_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ5497383.1"
                     /translation="MTVKINTKDGLIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQSLLRKENYAKGVVVKSTDLGISVDVYTVMSYGVKISEVSKNIQERVKFNLESQLG
                     LTADMVNVYVQNIKVVGEN"
     gene            7554..9218
                     /locus_tag="VHD02_000756"
     CDS             7554..9218
                     /locus_tag="VHD02_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEQ5497384.1"
                     /translation="MSNITTSLFQEMVQAAATRLGKQAEYVNSLNVFPVPDGDTGTNM
                     SMTMDNGAKEVADKPASTVGEVGQMLSKGLLMGARGNSGVITSQLFRGFGQSIKGKDE
                     LTGKDLAQAFQVGVEVAYKAVMKPVEGTILTVSRGAATAALKKADLTDDAVEVMQAAL
                     DGAKGALAKTPDLLPVLKEVGVVDSGGQGLVFIYEGFLSALNGDYVTSADFKATPANM
                     SEMINAEHHKSVVGHVATEDITYGYCTEIMVALKQGPTYVKEFNYDEFQGYLSSLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLIKIKVDNMRNQHEAQVQKTDVEKTKA
                     EVKDFGLIAVVAGEGLSEIFKAQGVDYVISGGQTMNPSTEDIVKAIEAVNAKQVIILP
                     NNKNIFMAAQSAAEVVDIPAAVVATRTVPQGFTSLLAFDPSKSLEDNVADMSTSLSDV
                     VSGSVTLAVRDTTIDGLEIHENDFLGMVDGKIIVSNPDMEATLKAAFEKMIDEDSEIV
                     TIFVGEEGDQDLAEELAGYLGETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            9455..10345
                     /locus_tag="VHD02_000757"
     CDS             9455..10345
                     /locus_tag="VHD02_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEQ5497385.1"
                     /translation="MLGPFIFIAFGVIVILAIVASTLYVVRQQSVAIVERFGRYQKTA
                     TSGIHVRLPFGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNVATQYRVNEQNVTDA
                     YYKLMKPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIV
                     KTLITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVG
                     IAQQRKAIVDGLAESIQELKEANISLNEEQIMSILLTNQYLDTLNTFAAKGNQTLFLP
                     NTPSGVEDIRTQVLSALKTK"
     gene            10574..11428
                     /locus_tag="VHD02_000758"
     CDS             10574..11428
                     /locus_tag="VHD02_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEQ5497386.1"
                     /translation="MKSKKVVSVISLTLSLFLVIGCAKVDNNKSVNRKPATKQTYNSY
                     SDDQLRSRENTMSVLWYQRAAETQALYLQGYQLATDRLKEQLNKPTDKPYSIVLDIDE
                     TVLDNSPYQAKNVLEGTGFTPESWDYWVQKKEAKPVAGAKDFLQFADQNGVQIYYISD
                     RSTTQVDATMENLQKEGIPVQGRDHLLFLEKGVKSKESRRQKVKETTNVTMLFGDNLL
                     DFADFSKKSQEDRTALLSDLQEEFGRRFIIFPNPMYGSWEGAIYKGEKLDVLKQLEER
                     RKSLKSFK"
     gene            11621..12817
                     /locus_tag="VHD02_000759"
     CDS             11621..12817
                     /locus_tag="VHD02_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEQ5497387.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGSKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAEFDQALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            13080..13631
                     /locus_tag="VHD02_000760"
     CDS             13080..13631
                     /locus_tag="VHD02_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEQ5497388.1"
                     /translation="MKTLRKLLSNYKFDIKKFKLGMRTLKTGLSVFLVLLVFHLFGWK
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMVNVACNNKSGIIGAVAALLIITLSIPTGQTFIYVTSRVFETFCGVFVA
                     ILVNTDVELIKNKWFNKKHSDVR"
     gene            13698..14069
                     /locus_tag="VHD02_000761"
     CDS             13698..14069
                     /locus_tag="VHD02_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEQ5497389.1"
                     /translation="MKEKELRRSMAVFPIGTVMTLTDLSARQIRYYEDQGLIKPERTQ
                     GNRRMFSLNDMDRLLEIKDFLSEGLNIAAIKREYVERQGKLMQKQKALTDADVRRILH
                     DEMLTQSGFSTPSQHIGNFRI"
     gene            14107..15453
                     /gene="glnA"
                     /locus_tag="VHD02_000762"
     CDS             14107..15453
                     /gene="glnA"
                     /locus_tag="VHD02_000762"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEQ5497390.1"
                     /translation="MAITVADIRREVKEKNVTFLRLMFTDIMGVMKNVEIPATKEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     KPFAGDPRGNLKRALKHMNEIGYKSFNLGPEPEFFLFKMDDKGNPTLEVNDNGGYFDL
                     APIDLADNTRREIVNILTKMGFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLV
                     VKTIAREHGLYATFMAKPKFGIAGSGMHCNMSLFDNQGNNAFYDETDKRGMQLSEDAY
                     YFLGGLMKHAYNYTAITNPTVNSYKRLVPGYEAPVYVAWAGSNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALAVLLEAGLDGIINKIEAPEPVEANIYTMTMEERNEAGIIDL
                     PSTLHNALKALQKDDVVQKALGYHIYTNFLEAKRIEWSSYATFVSQWEIDHYIHNY"
     gene            complement(15681..17363)
                     /locus_tag="VHD02_000763"
     CDS             complement(15681..17363)
                     /locus_tag="VHD02_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEQ5497391.1"
                     /translation="MTNISLKPNEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVDNLDRVKALVITHGHEDHIGGIPFLLKQANIPIYAGPLA
                     LALIRGKLEEHGLWREATVYEINHNTELTFKNMSVTFFKTTHSIPEPVGIVIHTPQGK
                     IICTGDFKFDFTPVGDPADLQRMAALGEEGVLCLLSDSTNAEIPTFTNSEKVVGQSIL
                     KIIEGIHGRIIFASFASNIYRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKGTFIEPSELKNLHASEALIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKVNNIHTSGHGGQQEQKLMLSLIKPKYFMPVH
                     GEYRMQKVHAGLAMDIGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRRDLSEDGVVLAVATVDFNTQMILAGPDILSRGFIYMRESGDLIRESQR
                     VLFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDKH"
     gene            complement(17365..17595)
                     /locus_tag="VHD02_000764"
     CDS             complement(17365..17595)
                     /locus_tag="VHD02_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEQ5497392.1"
                     /translation="MIYKVFYQETKDQSPRRESTKALYLNIDATDELDGRIKARRLVE
                     DNTYYNVEFIELLSDKHLDYEKETGVFELTEF"
     gene            17878..18576
                     /gene="tsaB"
                     /locus_tag="VHD02_000765"
     CDS             17878..18576
                     /gene="tsaB"
                     /locus_tag="VHD02_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEQ5497393.1"
                     /translation="MKTLAFDTSNKTLSLAILDDETLLADMTLNIQKNHSVSLMPAID
                     FLMTCTDLKPQDLERIVVAKGPGSYTGLRVAVATAKTLAYSLNIALVGISSLYALAAS
                     TCKQYPNTLVVPLIDARRQNAYVGYYRQGKSVMPQAHAPLEVIIEQLVEEGQLIFVGE
                     TAPFAEQIQKKLPQAILLPTLPSAYECGLLGQSLAPENVDAFVPQYLKRVEAEENWLK
                     DNEIKDDSHYVKRI"
     gene            18548..19003
                     /gene="rimI"
                     /locus_tag="VHD02_000766"
     CDS             18548..19003
                     /gene="rimI"
                     /locus_tag="VHD02_000766"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEQ5497394.1"
                     /translation="MIVTTLSESNMKTVEEQAKKIYQLLEMVYGTSPWTLEQVLIDIR
                     RDQTDYFLLYDHDKLLGFLAIQDLAGEVEMTQIAILPSHQGLGLASQLMTHLDSIESD
                     IFLEVRESNHRAQGLYQKFGFKLIGKRPDYYRNPIETALLMKREGKHDR"
     gene            18993..20021
                     /gene="tsaD"
                     /locus_tag="VHD02_000767"
     CDS             18993..20021
                     /gene="tsaD"
                     /locus_tag="VHD02_000767"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEQ5497395.1"
                     /translation="MTDRYILAVESSCDETSVAILKNESTLLSNVIASQVESHKRFGG
                     VVPEVASRHHVEVITTCFEDALQEAGISASDLSAVAVTYGPGLVGALLVGLAAAKAFA
                     WANHLPLIPVNHMAGHLMAAREQKPLVYPLIALLVSGGHTELVYVPEPGDYHIIGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMITEDHLEFSFSGLKSA
                     FINLHHNAKQKGDELILEDLCASFQAAVLDILLAKTKKALSRYPAKMLVVAGGVAANQ
                     GLRDRLAQEITHIEVVIPKLRLCGDNAGMIALAAAIEYDKQHFANMSLNAKPSLAFDQ
                     FPDSFVIN"
     gene            complement(20192..20461)
                     /gene="rpsN"
                     /locus_tag="VHD02_000768"
     CDS             complement(20192..20461)
                     /gene="rpsN"
                     /locus_tag="VHD02_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEQ5497396.1"
                     /translation="MAKKSKIAKYQKQLQLIEQYADLRRDLKAKGDYESLRKLPRDSN
                     PNRLKNRDKIDGRPHAYMRKFGVSRINFRDLAHKGQLPGVTKASW"
     gene            complement(20681..21439)
                     /locus_tag="VHD02_000769"
     CDS             complement(20681..21439)
                     /locus_tag="VHD02_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ5497397.1"
                     /translation="MSTNDLTKKLKKLKHVQVYNTIFQLIQDGTYSPGMQLPSEPELA
                     RQLNVSRMTLRKSLALLQEDHLIKNIRGKGNFILKTPETKYHQGFEYLQHPIYASLSS
                     DITKVELEYRIEVPTVAITASLKQETPVVIIVDRWYHSQNKAIAYSLSFIPIEVISKY
                     AINLNQEEPLLTFLEEKIYESGKASHSCNQIGYTKTGNYTATKYTLSENSAFILIQET
                     LYNGKDILVSTKHYVPADLFDLKVQSQSCQSDTH"
     gene            21679..22458
                     /gene="udp"
                     /locus_tag="VHD02_000770"
     CDS             21679..22458
                     /gene="udp"
                     /locus_tag="VHD02_000770"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEQ5497398.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            22479..23681
                     /locus_tag="VHD02_000771"
     CDS             22479..23681
                     /locus_tag="VHD02_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEQ5497399.1"
                     /translation="MQFIYSIIGILLILGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQIVSVVSTGVTSVINCGQAGLNFVFGSLADSGAKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILLPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGHLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRSTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            23711..24382
                     /gene="deoC"
                     /locus_tag="VHD02_000772"
     CDS             23711..24382
                     /gene="deoC"
                     /locus_tag="VHD02_000772"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEQ5497400.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECEDAIQNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAKCQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVGEGVKIKAAGGISSLEDAETFIALGASRLGTSRIIKIVKNEAI
                     KTDSY"
     gene            complement(24477..25646)
                     /locus_tag="VHD02_000773"
     CDS             complement(24477..25646)
                     /locus_tag="VHD02_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEQ5497401.1"
                     /translation="MTQYDTIIIGGGPAGMMAAISSSYYGYKTLLIEKNRRLGKKLAG
                     TGGGRCNVTNSGNLDDLMAGIPGNGRFLYSVFSQFDNHDIIAFFEENGVKLKEEDHGR
                     MFPTTDKSRTIIDALEKKIKALGGKVLTSTEVVSVKKQDDLFYLKSADQTFSCHKLIV
                     TTGGKAYPSTGSTGFGHDIARHFKLTVTDLEAAESPLLTDFPHKVLQGISLDDVTLSY
                     DKHVITHDLLFTHFGLSGPAALRLSSFVKGGEIAELDFLPHLSTDDLTAYLSDQRDKN
                     IKNALKGLLPERVADFLSEDYPEKVKQLSPKQEKELLDKLKHLQIPITGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVPGLYFAGEVLDINAHTGGFNITSALCSGWIAGKSS"
     gene            25851..26381
                     /locus_tag="VHD02_000774"
     CDS             25851..26381
                     /locus_tag="VHD02_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEQ5497402.1"
                     /translation="MGIEKTVSELADILGVSRQAVNNRVKSLPEEDLDKNEKGVTVVK
                     RSGLVKLEEIYKKTIFDDEPISEETKQRELLEILVDEKNTEITRLYEQLKAKDAQLAS
                     KDEQMRVKDVQIAEKDKQLDQQQQLTAKAMADKETLKLELEEAKAEANQARLQVEEVQ
                     AEVGPKKGFFTRLFAK"
     gene            26430..27017
                     /locus_tag="VHD02_000775"
     CDS             26430..27017
                     /locus_tag="VHD02_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEQ5497403.1"
                     /translation="MKHLDTYIAFDLEFNTVNDVSHVIQVSAVKYDHHKEVDSFDTYV
                     YTDVPLQSFINGLTGITSNKIAAAPKVEEVMAAFKNFVGELPLIGYNAQKSDLPILAE
                     NGLDLRDQYQIDLFDEAYDRRSADLNGIANLRLQTVATFLGIKGRGHNSLEDARMTAA
                     IYKSFLETDTNKAYLSQQEEVTTDNPFAALGDFFD"
     gene            27169..27906
                     /locus_tag="VHD02_000776"
     CDS             27169..27906
                     /locus_tag="VHD02_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEQ5497404.1"
                     /translation="MSQFYSTGELANLAGVSIRTVQYYDQRGILIPTALTAGGRRLYT
                     DSDLEQLRMICFLRDLGFSIEQIRKVLAEENAAQVLELLLVDHIATAKEDLAAKEQQV
                     DIAVKILDRLRKQDPQSLDFLMDISLSMKNQKAWKKLQWKMGISIFLAIVSYVGLILL
                     SNYLQKFWLVYVAMGIFLPGFSWLVIYFRKQFIYLCPHCHQKFEPSFNEFALAGHTPR
                     TRKLTCSHCHQKSYCLELAKEDTVSRG"
     gene            complement(27963..28262)
                     /locus_tag="VHD02_000777"
     CDS             complement(27963..28262)
                     /locus_tag="VHD02_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEQ5497405.1"
                     /translation="MMNMQNMMKQAQKLQKQMEQKQADLAAMQFTGKSAQDLVTATFT
                     GDKKLVGIDFKEAVVDPEDVETLQDMTTQAINDALTQIDEATKKTLGAFAGKLPF"
     gene            complement(28455..28829)
                     /locus_tag="VHD02_000778"
     CDS             complement(28455..28829)
                     /locus_tag="VHD02_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ5497406.1"
                     /translation="MSAKKTFFASNLKYLRLKKNMEQLELANLLGRKSSSSISEWEKG
                     KYTPKSGLLSDIAAIFDVSLTALMEEDLTLESKGVSYQLPQRELAMISEQLSENNYTK
                     WVDFAQILLEQQAKEDRELDNH"
     gene            28999..29220
                     /locus_tag="VHD02_000779"
     CDS             28999..29220
                     /locus_tag="VHD02_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ5497407.1"
                     /translation="MTKSTLKELRTTKKMTQQELSHLTGISVRTIARYEKDTKKLRRA
                     KYEKLKGIAQALAVSVDDIFLGIDSEFMN"
     gene            complement(29300..31582)
                     /locus_tag="VHD02_000780"
     CDS             complement(29300..31582)
                     /locus_tag="VHD02_000780"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEQ5497408.1"
                     /translation="MRYNQFSYIPTSLERAAEELKELGFDLDLQKTAKANLESFLRKL
                     FFHYPDSDYPLSHLIAKNDMDALSFFQSEQELSKEVFDLLALQVLGFIPGVDFTEADA
                     FLDKLAFPIHFDETEIIKHIHHLLATRCKSGMTLIDDLVSQGMLTMDNDYHFFNGKSL
                     ATFDTSQLIREVVYVEAPLDTDQDGQLDLIKVNIIRPQSQKPLPTLMTPSPYHQGINE
                     VANDKKLYRMEKELVVKKRRQITVEDRDFIPLETQPCKLPIGQNLESFSYINSYSLND
                     YFLARGFANIYVSGVGTAGSTGFMTSGDYAQIESFKAVIDWLNGRATAYTSHSKTHQV
                     RADWANGLVCTTGKSYLGTMSTGLATTGVDGLAMIIAESAISSWYNYYRENGLVCSPG
                     GYPGEDLDVLTELTYSRNLLAGDYLRHNDHYQELLNQQSQALDRQSGDYNQFWHDRNY
                     LKNAHQIKCDVVYTHGLQDWNVKPRQVYEIFNALPSTINKHLFLHQGEHVYMHNWQSI
                     DFRESMNALLCQKLLGLANDFSLPEMIWQDNTCPQNWQERKVFGTSTIKELDLGQELL
                     LIDNHYGEDEFKAYGKDFRASKAALFKGKANQALIDILLEEDLPINGEIVLQLKVKSS
                     ENKGLLSAQILDYGKKKRLGDLPIALTQSSIDNGQNFSREPLKELPFREDSYRVISKG
                     FMNLQNRNNLSSIETIPNNKWMTVRLPLQPTIYHLEKGDTLRVILYTTDFEHTVRDNS
                     NYALTIDLSQSQLIVPIASN"
     gene            complement(31604..32263)
                     /locus_tag="VHD02_000781"
     CDS             complement(31604..32263)
                     /locus_tag="VHD02_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEQ5497409.1"
                     /translation="MNHHILQRYIDNHNFPIIEKSYHKYLTFESLEEDFTYILKDGIV
                     KQSVLSKYGMEFNLRYVTGLEITSVLNTGYSKDMGEPYNVRIESEKASFYKVRRSAFL
                     KDINEDIELQGYVKDFYHNRLQKSMKKMQCMLTNGRIGAISTQIYDLMTLFGEELPNG
                     QILINFVITNEELGKFCGISTASSVSRILKQLKEKNIIRIDKQHIIITNLDKLKDNIV
                     F"
     gene            32429..33625
                     /locus_tag="VHD02_000782"
     CDS             32429..33625
                     /locus_tag="VHD02_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ5497410.1"
                     /translation="MEEKLFNKHFVAITVINFIVYMVYYLFTVIIAFVATRELGAQTS
                     QAGLATGIYILGTLLARLIFGKQLEVFGRRLVLRGGAIFYLLTTLAYFYMPTISMMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPARKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HIDFRMVIVLCSVLIGCVVVGAFAFPVKNMSLNAEQLAKTKSWTVDSFIEKKALFITA
                     IAFLMGIAYASVLGFQKLYTSEIHLTTVGAYFFVVYALIITITRPAMGRLMDAKGDKW
                     VLYPSYLFLAMGLFLLGSVSSGGSYLLSGALIGFGYGTFMSCGQAASIQGVDEHRFNT
                     AMSTYMIGLDLGLGAGPYLLGLIKDLALGSGVASFRHLFWLAAVIPLICTLLYLLKTK
                     TRQVVS"
BASE COUNT        10684 a         5728 c         7117 g        10485 t
ORIGIN      
        1 catagtcatc cgttgtgata atgcttgaaa atagtcagtt agtaagcgaa aaagtctaac
       61 attaaccttt ataaattgaa aataactgtt agtagactaa tgacaggtag atactcttaa
      121 tagcgcgttt gatttcacgc ataaaaagct ttaaaaacgc tttaaattat gataaaatga
      181 actgtatcga aattatttta aggagaaatg acttaatgtc tacatcattt gaaaacaaag
      241 ctacaaatcg tggcgtgatt acatttacaa ttagtcaaga taaaataaaa ccagctcttg
      301 ataaagcttt taataaaatc aaaaaagact taaatgcacc aggtttccgt aaaggacaca
      361 tgcctcgtcc agtcttcaac caaaaatttg gtgaagaagt tctttatgaa gatgctttga
      421 atattgtatt gccagaagct tatgaagcag ctgtgacgga acttggtctt gatgtggttg
      481 cacaaccaaa aatcgatgtt gtgtcaatgg aaaaagggaa agagtggaca ctttctgctg
      541 aagttgtgac aaaacctgaa gtgaaacttg gtgattacaa aaaccttgtt gtagaagttg
      601 atgcttcaaa agaagtttca gatgaagacg tggatgctaa aattgagcgc gaacgtcaaa
      661 accttgcgga actcattatt aaagacggtg aagcagctca aggtgacact gttgtgattg
      721 actttgttgg ttcagttgat ggtgttgagt ttgatggcgg taaaggagat aacttctctc
      781 ttggacttgg ttcaggacaa tttatcccag gttttgaaga tcaactagtt ggtgctaaag
      841 ctggcgatga agtagaagtt aatgtcacat tcccagaatc ttaccaagca gaagatcttg
      901 caggtaaagc cgctaaattt atgacaacta ttcacgaagt caaaacaaaa gaagtaccag
      961 agcttgatga tgagcttgca aaagatattg atgaagatgt tgacacactt gaagacttaa
     1021 aagtaaaata tcgtaaagaa cttgaagcag ctcaagaaac tgcttatgat gatgctgttg
     1081 aaggagctgc gattgaatta gcagttgcaa atgctgaaat tgttgattta cccgaagaaa
     1141 tgattcatga agaagtcaac cgttcagtga atgaatttat gggcaacatg caacgtcaag
     1201 gaatctcacc tgaaatgtac ttccaattga ctggtacaac tcaagaagat ttacataacc
     1261 aatattcagc tgaagctgac aaacgtgtta aaacaaacct tgttattgaa gcaattgcta
     1321 aagcagaagg ttttgaagca acagatagtg aaattgaaca agaaattaat gaccttgcaa
     1381 cagaatataa catgccagct gaccaagttc gttctcttct ttcagcagat atgttgaaac
     1441 atgatattgc aatgaaaaaa gcagttgaag tgattacaag cacagcaagc gttaagtaat
     1501 cttattaagg aacaaaacgt acaagccgga ttagaacgtc tttctagtct ggtttttctt
     1561 tttctaaaga taaggaatta gttaaacgtt attttaatga attagcttta aagatttgtc
     1621 aagaagaagt gatatgagtt gataaaagtt ttgacgaatt acttttttta gcgtaaaatg
     1681 gtagagaatg ataaaataag gagaactgcc ttgaaattag acgtatttgc aggacaagaa
     1741 aaaagcgagc tttccatgat tgaagtggcg cgtgccattt tggaagaacg cggtcgcgac
     1801 aatgagatgt actttagcga tttagtcaat gagattcaaa attacttagg aaaatctgat
     1861 gcaggtattc gtcatgcatt accatttttc tatactgact taaatacgga tggctcattt
     1921 attcctctag gtgaaaacaa atggggactt cgttcgtggt atgctattga tgaaattgat
     1981 gaagaaatta ttactctaga agaagatgaa gatggcgcac aaaaacgtaa gaaaaaacgc
     2041 gttaatgctt tcatggatgg agatgaagac gctattgatt atagagatga tgatccagaa
     2101 gatgaagatt tcacagaaga atcagctgag gttgaatatg atgaagaaga tccagatgat
     2161 gaaaaatcag aagtggagtc atatgattca gaattgaatg aaattatccc tgaagatgat
     2221 tttgaagaag ttgatatcaa tgaagaagac gaagaagatg aagaagatga agaaccagtt
     2281 ctctaatgtc tttagttcag aaataaaaat gtgattatca tttgacaaat tgtctattat
     2341 agtttatatt attattcggg cacctcttta tgaggtcagg caaaagctct ctagttaata
     2401 gggggctatt tttgttttct agaataatgc cagtttgcct ctatatttag aaaactaagg
     2461 tgatacctaa ccaatgtgga accaactaat agggaattaa caagaaagca atgcttttat
     2521 tcagcttata taaaggagta ctatgacgaa atacattttt gtaactggtg gtgttgtatc
     2581 atcaattggc aaaggaattg ttgcggcaag tcttggccga ttgttaaaaa atcgtggact
     2641 taaggtgacg attcaaaaat ttgatcctta tattaatatt gatccaggga cgatgagccc
     2701 ctatcagcat ggagaagtat atgtgacaga tgacggtgct gaaaccgact tggatttggg
     2761 tcactatgaa cgttttattg atatcaatct taataaatat tccaatgtta caacaggtaa
     2821 aatttatagt gaagtccttc gtaaagaacg taaaggtgag taccttggag cgactgttca
     2881 ggtgattcct catatcacag atgcccttaa aaaaaaaatc aaacgcgcag cttcaacaac
     2941 ggactctgat gtaattatta ctgaggtcgg ggggactgtt ggcgatattg aaagtttacc
     3001 gttcttagaa gctcttcgtc agatgaaagc tgatgttggt tctgaaaatg tgatgtatat
     3061 tcacaccacc ttgttgccat atttaaaggc agcgggtgaa atgaaaacta agccaacgca
     3121 acattccgtt aaagaattgc gtggtttagg gattcaaccg aatatgttgg ttattcgtac
     3181 ggaagagcca gttgagcagg ggattaaaaa taaattggct cagttctgtg atgtgaattc
     3241 agaagctgtt atcgagtcac gtgatgtaga acacttgtat caaattccat tgaacttgca
     3301 agcccaatca atggatcaga ttgtttgtga tcatttgaaa ttgaatgcgc cacaagctga
     3361 tatgactgag tggtcagcta tggttgacaa ggtgatgaat ttaagaaaaa caacaaaaat
     3421 tgccttggtt ggtaaatatg tggaattgcc agatgcctac ttatctgttg tggaagcctt
     3481 gaaacattct ggttatgcca atgataccgc aattgaccta aaatgggtca atgctaacga
     3541 tgtgacggtg gataatgcag ctgacttgtt gggagatgcg gatgggatta ttgtaccagg
     3601 tggttttggt caacgtggta cagaaggcaa gatccaagcg attcgctatg cccgcgagaa
     3661 tgatgtgcca atgcttggta tctgtcttgg catgcaactg acttgtgttg aatttgctcg
     3721 ccatgtcctc aatatggaag gtgcaaactc ttttgaatta gagccaagta ctaaataccc
     3781 catcattgat atcatgcgtg atcaaattga tattgaagat atgggaggta cgcttcgtct
     3841 gggattgtac ccatgtaaat tgaaaccagg ttcaaaagca gcaatggctt acaataatca
     3901 agaggtggtg caacgtcgtc atcgtcaccg ttatgagttc aacaacaaat tccgtccaga
     3961 atttgaagca gctggttttg ttttctcagg ggtatcacct gacaaccgct tagttgaaat
     4021 tgtagaattg aaagagaaaa aattctttgt agcagctcaa taccacccag aattgcaaag
     4081 ccgtccaaat cgtcctgagg agctatacac cgcttttgtt acagctgcta ttaaaaatag
     4141 caattagtgg ttttaagggt aagtttgtgg cattctactt ggctggcaga tactgataaa
     4201 gattactagc tattagaggg atcttttcta taaaaatagc attagaaaaa gaaaaacgtg
     4261 atatttttag gttagcactt tctttttcag tcattttttt gtaaaatact tggtatgaaa
     4321 actattcgaa tagcaaagta tttaggaatt ctattccttt tgataactct tattagcgta
     4381 ggtgctagtt tttatttctt tcatgttgca caaataagag aagagaaatc gtttattaat
     4441 aataagaaac ggagtacaaa taatccatta tacccagctg aacagtcttt tgacgcttta
     4501 ccttacgaaa aacgtcaact aacaaatcgt gggttaaaac aagtggggtg gtacttacca
     4561 gctgctcaaa aaacaaaaaa gacagctatt gttgttcatg gttttacgaa tgacaaagaa
     4621 gatatgaagc catatgccat gctttttcat gatttgggct ataatgtctt aatgccagac
     4681 aatgaggccc atggggaaag tgaagggaac ttgattggtt atggctggaa tgaccgcctt
     4741 aatgtcatgg cttggacaga ccaactgatt aaggaaaacc ctgaaagcca aatcacactc
     4801 tttggcttat ctatgggtgc tgcaacagta atgatggcaa gtggtgagcg attgcctgcg
     4861 caagtcacct ccctcatcga agattgcggt tataccagtg tttgggacga attgaagttt
     4921 caggccaagg ctatgtacaa cttgcctgcc tttcctttac tctatgaagt ctctgcctta
     4981 tctaagattc gagcaggttt tagttacgga gaagcgagct cagtgaaaca gctggctaaa
     5041 aataaacgtc caactttatt tatccacggt gataaggatg attttgttcc tacaaaaatg
     5101 gtttatgaca attataaggc cacgaaaggt cctaaggaaa tcttgattgt taaaggggca
     5161 aaacacgcca aatcctttga aacaaaccca gaacaatacc agaaaaaaat tgccgctttt
     5221 ttgaaaaaag ttgagaaata agtattgaca gttaaactca cagttgctat aataatttat
     5281 gttactgatg agtagcgggg atggcggaat tggcagacgc gcaggactaa ggatcctgtg
     5341 accgctttag gtcgtgtggg ttcaagtccc actctccgca taatgtaaga ggtgaatcat
     5401 tgattcgtct ctttttttgg ttaaatgaag gacaagaagg catagattta ctggagaaaa
     5461 cttttgataa tctgcttttg gaaaacgctt taagcttgtg aaagactaga aatttatgga
     5521 aaattgtgct agaatgaaca atgtaaacgg ggagttatcc cgaaaaataa gaggaggcct
     5581 tacaaatggc aatcgtttca gcagaaaaat ttgtccaagc agctcgtgaa aacggatatg
     5641 ctgttggtgg atttaacaca aacaacttag agtggactca agcaatcttg cgtgcagcag
     5701 aagctaaaca agctccagtt cttatccaaa catcaatggg tgcagcaaaa tacatgggtg
     5761 gttataaagt atgtcaaact cttattacta accttgttga gtcaatgggt atcactgttc
     5821 ctgttgctat ccaccttgat catggtcact acgaagatgc tcttgagtgt atcgaagtag
     5881 gttacacttc aatcatgttt gacggttcac accttccagt tgaagaaaac cttgctaaaa
     5941 ctgcagaagt tgttaaaatt gcacatgcta aaggcgtttc agttgaagct gaagttggta
     6001 caatcggtgg tgaagaagac ggtatcattg gtaaaggtga gctcgctcca atcgaagatg
     6061 ctaaagcaat ggttgaaact ggaattgact tcttggcagc tggtatcggt aacatccacg
     6121 gtccataccc agaaaactgg gaaggtcttg ctcttgacca cttagaaaaa ttaacagcag
     6181 ctgtaccagg tttcccaatc gtattgcacg gtggttctgg tattcctgac gatcaaatca
     6241 aagaagcaat ccgtcttggt gttgctaaag ttaacgttaa cactgaaagt caaatcgctt
     6301 tctctaacgc aactcgtgaa tttgctcgta actacgaagc taacgaagca gaatatgatg
     6361 gtaaaaaatt atttgaccca cgtaaattct tggctccagg tatgaaagct gttcaaggtg
     6421 ctgttgaaga acgtatcgat gttttcggat cagctaacaa agcataattt agagtattaa
     6481 atactctaaa gccctcttga gggctttttt agatacaatt gacaccctaa aatggatacc
     6541 agtttctgtg aaacaatctt tgattgtgaa caaacaaagc ataatttaga gtattaaaat
     6601 ctggaccctg ctatctatgg taagtctttt tgttattgtt aaagaggtta agcggaatgt
     6661 taatatgttt tagctaaaaa atgtagtgta aaatgagtac gtagactatt gtaatagtaa
     6721 ttccgtaaaa ttgttgaaaa agaaaatggg aaataccttg tcaaattaag caccctatga
     6781 tagaatagat aggtatgtgg tgctagcaca tccgtaaaca tattctacgt aggaggaaac
     6841 agatcaaaat ggctaaagta tgttatttta caggacgtaa aaccgtttct ggaaacaacc
     6901 gttcacacgc gatgaaccaa acaaaacgta cagttaaacc aaaccttcaa aaagttacta
     6961 tccttgttga tggtaaacct aaaaaagttt gggcttctgc tcgtgcgctt aaatctggta
     7021 aagtagaacg catctaatag aaataagcct tagggctttt tttattagct tatttctatc
     7081 cataagctag tcagggtaat aaacgctatc gttaactatt ctatctcttt tacattactt
     7141 ctaaaatatg ataaaatagg atgataaaaa ctttttgagg tagtaactat gactgtaaaa
     7201 attaatacaa aagatggtct aatcgagcta tccgacgatg tgattgcaac tgtcgtggga
     7261 ggatcagcaa cggaaatttt tggtgttgtt ggaatggcca gcaaaagtgc aataaaagac
     7321 aattttcaat cactacttcg caaagaaaac tacgctaagg gtgttgtagt aaaatcaaca
     7381 gatctgggta tttctgttga tgtctacact gtgatgagtt atggtgtgaa aatcagtgaa
     7441 gtctctaaaa atatccaaga acgcgtgaaa tttaaccttg aaagtcagct tggtctcact
     7501 gctgatatgg tcaatgttta cgtgcaaaat attaaggttg taggagaaaa ttagtgtcaa
     7561 atattacaac aagcttattc caagaaatgg tccaagcggc agcgactcgc cttggcaaac
     7621 aagcagaata tgttaattcc ttaaatgttt ttcctgttcc agatggtgat acaggaacaa
     7681 acatgagtat gactatggac aatggtgcca aggaagttgc tgataaacct gcgtcaactg
     7741 tgggagaagt tggacaaatg ttatcaaaag gtcttttgat gggagcgcgt ggaaattctg
     7801 gagttattac ttcacaactt tttcgtggtt ttggtcaaag tattaaggga aaagatgagc
     7861 tgacaggtaa agatttggca caagcctttc aagtgggtgt agaagttgcc tacaaagcag
     7921 tgatgaaacc tgttgaaggg acaatcttga cagtatctcg tggagcagca accgctgcct
     7981 taaaaaaagc agatcttaca gacgacgccg tggaagtcat gcaagctgct ttagacggtg
     8041 ccaaaggtgc ccttgctaag acacctgatt tattaccagt ccttaaagaa gttggtgtgg
     8101 ttgattcagg cggtcaaggt cttgtcttta tctatgaagg tttcctatct gctttgaatg
     8161 gcgactatgt gacttcagca gactttaaag cgactcctgc taacatgtct gaaatgatta
     8221 atgctgagca tcacaaatcc gttgttggtc atgtggctac tgaagacatt acctacggtt
     8281 attgtactga aatcatggtt gcccttaaac aaggcccaac ctatgtgaaa gaattcaact
     8341 atgatgagtt tcaaggctac cttagtagcc ttggtgactc gctgttagtg gtcaatgatg
     8401 atgagattgt taaagttcac gtccatactg aagaccccgg acttgtgatg caagaaggtc
     8461 ttaaatatgg ttcgctcatc aagattaaag tggacaatat gcgtaaccag catgaagcac
     8521 aggttcaaaa gactgatgtc gaaaaaacca aggctgaagt aaaagacttt ggcttgattg
     8581 cagtagtagc tggagaaggt ttatctgaaa ttttcaaggc gcaaggtgtt gattacgtga
     8641 tttctggtgg tcaaaccatg aatccatcta cagaagatat tgttaaagct attgaagcag
     8701 ttaatgccaa acaagtcatt atcttgccaa acaataaaaa tatctttatg gctgcccaat
     8761 ctgctgcaga agtggtggat atcccggctg ctgttgttgc aacacgtact gttccgcaag
     8821 gatttaccag cttgttggcc tttgatcctt caaaatcttt agaggacaat gtagcagata
     8881 tgtcaactag tctttctgac gtggtcagtg gaagcgttac tttggcagtc cgtgacacca
     8941 ccattgatgg tttagaaatc catgaaaatg atttcttggg aatggttgat ggtaaaatca
     9001 tcgtttcaaa cccagacatg gaagcaactc taaaagctgc ctttgaaaag atgattgacg
     9061 aagatagcga gattgtgact atctttgttg gtgaagaagg tgatcaagac ttagcagaag
     9121 aacttgctgg gtatttagga gagacttacg aagatgtgga agttgagatc catcaaggag
     9181 atcagcctgt ttacccatat ctcatgagtg tagaataata gaatacaata aaaaaagagg
     9241 agttaggcaa aggtctgact ctttttgtct atattgatgt cgtaatgagt ggttttagtg
     9301 atggtaagcc cctaaaaaat gagggtaatc catcacaaag ctaatggtta cgatttaatt
     9361 tgacaaaacc tagaaaaaca ttataattgt attataacaa taagtcaaga actgaaaacg
     9421 gttcagttct aagatatgac aaggaggaga ttttatgtta ggaccattta tctttattgc
     9481 ttttggggtt attgtaatcc tagctattgt tgccagtacc ctttatgttg ttcgtcaaca
     9541 atctgttgcc atcgtggaac gttttggtag gtaccaaaaa acagccacga gtggtattca
     9601 tgtccgcctt ccgtttggga ttgacaaaat tgctgcgcgt gtgcagctgc gtcttttaca
     9661 gtcagaaatc atcgttgaaa ccaaaaccaa ggacaatgtg tttgtaactt tgaatgttgc
     9721 cactcagtat cgtgttaacg aacaaaatgt aacagatgct tactacaaat taatgaaacc
     9781 tgaatctcaa atcaagtctt atattgaaga tgccttgcgg tcatccgtgc cgaagttgac
     9841 cttagacgaa ttgtttgaga aaaaagatga aattgccttg gaagtacaac atcaagtggc
     9901 agaagaaatg tcaacctatg gatatattat tgtcaaaacc ttgattacca aggtggagcc
     9961 tgatgctgaa gtcaaacagt caatgaatga aatcaatgcg gctcaacgca aacgtgtagc
    10021 tgctcaagag ttggcaaacg cagataaaat taaaattgtg acagcagcag aagcagaggc
    10081 tgaaaaagat cgattgcacg gggtagggat tgcccaacag cgtaaggcca ttgtggatgg
    10141 tttggcggag tctattcaag agcttaaaga agcgaatatt tccttaaacg aagaacaaat
    10201 catgtccatt ttgttgacca accaatacct ggataccctc aatacctttg cggcaaaagg
    10261 aaaccaaacc ctttttctac caaatacacc aagtggggta gaggatatcc gtacacaagt
    10321 tttatctgcc cttaaaacaa aataacgtgt aaaagcttgg ctaaaaagtt gctcaatact
    10381 ggatttagca gaaggctatc ataccttgtg aagtggcaaa cgacgaaatg aatacattac
    10441 ttttattgga gaaatgaaag taaaaaagcc tttttcatga ttaacaacat agtattgatt
    10501 aaatgttaag ttgtattgac ttttaaatta aataagttta aaatcaactt aataagcaaa
    10561 aaggagattt tcaatgaaat ccaaaaaagt tgttagtgtt atatcactta ccttatccct
    10621 ttttttggtg ataggttgtg ctaaagttga taacaacaaa tcagttaatc gtaagcctgc
    10681 tactaaacaa acctataata gctatagtga tgaccaatta agatcgcgtg aaaataccat
    10741 gtctgtttta tggtaccagc gagctgcgga aactcaagcg ctttatttac aggggtatca
    10801 gttagcaacg gatcgcttaa aagaacaact caataaacca acggataaac cttattcaat
    10861 tgtattagat attgacgaaa cagttcttga taatagccct tatcaagcta aaaatgtttt
    10921 ggaaggaaca ggatttacac ctgaaagctg ggattattgg gtacaaaaga aagaagcaaa
    10981 accggttgct ggtgctaaag actttttgca atttgcagac caaaatggtg ttcaaattta
    11041 ctacatttca gacagatcaa ctactcaagt agatgctaca atggaaaatc tccaaaaaga
    11101 aggtattcca gtacaaggtc gtgatcatct tctattctta gaaaaaggcg taaaatcaaa
    11161 ggagagtcgt cgtcaaaagg tcaaagaaac aactaatgta acgatgctat ttggtgataa
    11221 tcttctagat tttgctgatt tttcaaaaaa atctcaagaa gatagaacag ctttattatc
    11281 agatttgcaa gaagagtttg gaagacgctt tatcattttc cctaatccta tgtatggttc
    11341 atgggaaggt gccatttata aaggtgaaaa gctggatgtg cttaagcaac tagaggaacg
    11401 ccgtaaaagt ttaaaaagct ttaaataaag taattgaaaa agaggtgcaa aagaaaatgt
    11461 aacctctttt taagtactat tattttctga aaatagggtt aatgactgaa aaaaatgttg
    11521 attttgtgaa atgattattt tattattttc atattttatc tttatgctag gaattgtggt
    11581 ataatggagg agtataaaaa atattaagga gtctattcac atggctaaat tgactgttaa
    11641 agacgttgat ttgaaaggta aaaaagtcct cgttcgtgtt gactttaatg tgcctttgaa
    11701 agatggcgtt atcactaacg acaaccgtat cactgcggct cttccaacaa tcaagtatat
    11761 catcgaacaa ggtggtcgtg ctatcctctt ctctcacctt ggacgtgtta aagaagaagc
    11821 tgacaaagaa ggaaaatcac ttgcaccggt agctgctgat ttagctgcta aacttggtca
    11881 agatgttgta ttcccaggtg ttactcgtgg ttcaaaatta gaagaagcaa tcaatgcttt
    11941 ggaagatgga caagttcttt tggttgaaaa cactcgtttt gaagatgttg acggtaagaa
    12001 agaatctaag aatgacgaag aacttggtaa atactgggct tcacttggag atggaatctt
    12061 cgttaacgat gcatttggta cagcacaccg tgctcacgca tcaaacgtag gtatttcagc
    12121 aaacgttgaa aaagctgtag ctggtttcct tcttgaaaac gaaattgctt acatccaaga
    12181 agcagttgaa actccagaac gtccattcgt agctattctt ggtggctcaa aagtttctga
    12241 taagattggt gttatcgaaa atcttcttga aaaagctgat aaagttctta tcggtggtgg
    12301 tatgacttac acattctaca aagctcaagg tatcgaaatc ggtaactcac ttgtagaaga
    12361 agacaaattg gatgttgcta aagacctcct tgaaaaatca aatggtaaat tgatcttgcc
    12421 agttgactcg aaagaagcaa acgcatttgc tggttacact gaagttcgcg acactgaagg
    12481 tgaagcagtt tcagaaggct tccttggtct tgacatcggt cctaaatcaa tcgctgagtt
    12541 tgaccaagca cttactggtg ctaaaacagt tgtttggaac ggacctatgg gtgtctttga
    12601 aaaccctgac ttccaagctg gtacaatcgg tgtcatggac gctatcgtta aacaaccagg
    12661 cgttaaatca atcatcggtg gtggtgattc agcagcagct gctatcaacc ttggccgtgc
    12721 tgacaaattc tcatggatca gtaccggcgg aggcgctagc atggaacttc tcgagggtaa
    12781 agtattacca ggtttggcag cattgactga aaaataatag tcattataat atatagtaaa
    12841 gggcatgctg atgcatgtct ttttttagtt gatcatgtac agttattgaa ctttccctaa
    12901 cttcacttat catttactag tttttagata tagtggtatt tggctgacac tgcaattttc
    12961 aaaagtttat gacgatttca tatcaattgg tttataaaag atatcctaat cataaataaa
    13021 acgtttacca aaagggtttt atggattgat atgttaaaat agatagtgaa gggataggta
    13081 tgaaaacgtt aagaaaatta ttatctaatt acaagtttga tattaaaaag tttaaattgg
    13141 gaatgagaac attgaaaaca ggcttatcgg tctttttagt tttattggtt tttcatttat
    13201 ttggttggaa agggcttcaa attggtgctc taactgctgt atttagttta agagaagact
    13261 ttgataaaag cgtgcacttt ggcttttcta gaattattgg taatagtata ggtggcctac
    13321 tctccttagt attttttgca ttcaatgaga tatttcatca agctttttgg gttactttat
    13381 taattgttcc tatttgtacc atgttatgta tcatggttaa tgttgcatgt aataataaat
    13441 caggtattat tggggcagtg gctgctcttt tgattattac actgtctatt ccaacaggac
    13501 aaacgtttat ttatgtgact tcgcgggtat ttgaaacatt ttgtggcgtc tttgtagcta
    13561 ttttagttaa tactgatgtt gaacttatta aaaataaatg gtttaacaaa aaacacagtg
    13621 atgttagata atataacata tatacttgac aggttatttt tttcagccta taataaggaa
    13681 gatgaaagga gtttgtcatg aaagaaaaag aacttagacg ctcaatggct gtttttccta
    13741 ttggaacggt gatgacatta acagatctct ctgcgagaca aattcgctat tatgaagatc
    13801 aaggtttaat taagcctgaa cgaacgcaag ggaatcggcg tatgttttcc ttaaatgaca
    13861 tggaccgact tttggaaatt aaagattttt tgtctgaggg attaaatatt gcagctatta
    13921 aacgcgaata tgtcgagcgt caaggtaagt taatgcaaaa acaaaaagct ctaacagatg
    13981 cagatgtcag acggatctta catgatgaaa tgctgacaca aagcggtttt tctactccct
    14041 cacagcatat tggtaatttt cgtatttaag atatgaattg tataaaattt aaaggagacc
    14101 tatttaatgg caataacagt agctgacatt cgtcgtgaag tcaaagaaaa aaatgtaacg
    14161 tttcttcgct tgatgttcac tgatatcatg ggcgttatga aaaatgtgga gattcctgca
    14221 actaaagaac agttagacaa agtattgtct aacaaggtta tgtttgatgg ttcatctatc
    14281 gaaggttttg tacggatcaa tgagtcagat atgtaccttt accctgattt agacacttgg
    14341 attgtttttc cctggggaga tgaaaatgga gcagttgcag gtttaatttg tgatatttat
    14401 acagcagaag gaaagccttt tgcaggagat cctagaggaa atttaaaaag agccctgaaa
    14461 cacatgaacg agatcggcta caaatcattt aatcttggac cagaaccaga atttttcctt
    14521 tttaagatgg atgataaagg taatccgaca cttgaagtta acgataatgg tggttatttt
    14581 gatttagcgc caattgactt agcagacaac acgcgccgtg aaattgtgaa tattttaacg
    14641 aaaatgggtt ttgaagtgga agctagtcat catgaagtgg ctgttggtca acatgagatt
    14701 gattttaaat atgcagatgt tttgaaagct tgtgataata ttcaaatttt taagctagtt
    14761 gtaaaaacga ttgcccgtga acatggactt tatgctactt tcatggctaa accaaaattt
    14821 ggaatagctg gatcagggat gcactgtaac atgtctttgt ttgataacca aggtaataat
    14881 gctttttatg atgaaactga taagcgaggg atgcagctat cagaagatgc ttattatttc
    14941 ttgggaggac taatgaagca tgcttataac tacactgcta tcactaaccc tacagtgaat
    15001 tcttacaaac gattagttcc gggttatgag gcacctgttt atgtcgcttg ggctggaagt
    15061 aatcgttcac cgcttatccg tgttccagca tcacgtggta tgggaacgcg tttggagtta
    15121 cgttcggttg atccgacagc taatccttat ttagccttgg ctgttctctt ggaagctgga
    15181 ttagatggta tcattaacaa aattgaagct ccagaacccg ttgaagctaa tatttatacc
    15241 atgacaatgg aagaacgaaa tgaagcaggc attattgatt tgccatcaac gcttcataat
    15301 gccttaaaag ctcttcaaaa agatgatgtg gtacaaaagg cactaggata ccatatctac
    15361 actaatttct tagaagcaaa acgaattgaa tggtcttcct atgcaacttt tgtttctcaa
    15421 tgggaaattg accattatat tcataactat tagagttatc aggacctgct caagttgagt
    15481 gggtcttttg gatgagctat tgtatttgag ctagcaagct attattagct gatcggacac
    15541 caattgattt tccattatag aaaactaaaa accagtccat gttctatgag gactggtttt
    15601 tagtttgata attatctgaa ctagaactga tatgttaaag atatagacag atagtattaa
    15661 gaaatgagtc ttatttgatt ttagtgctta tcaggtgtta agaccatcgg aattataatt
    15721 ggttcacgct cagtcttttc atacaagaat ggacgaagag cattgacgat agcaccgtta
    15781 actgattgga tgctagcatc cttatttttt aaagcaatac ggatagcgtt aaagagcaca
    15841 cgttggcttt cgcggatcaa gtcacctgac tcacgcatat aaatgaaacc acggctcaga
    15901 atatctggtc cagctaaaat catttgtgtg ttaaaatcaa cagttgcaac agctaagaca
    15961 acaccatctt ctgacaaatc acggcggtct cgtaaaacgg ctgctccaat atccccgatg
    16021 ccattaccat ccacgtagat gtcttgggca ttaaagtgac cagcaatacg cgcactgtca
    16081 ctggttaaag ctagaacgtc tccattttcc ataataaaga tattttcttt tggaatacca
    16141 atatccatcg ccagtcctgc atgaactttt tgcatgcgat actcaccatg tactggcatg
    16201 aaatattttg gtttaatcaa actaagcatg agcttttgtt cttgctgtcc tccatgacca
    16261 gaagtatgaa tattattgac ttttccatga ataacatcaa caccagcttc ttggatggta
    16321 ttgattaatt tattgacact tgtggtatta ccagggattg gacttgatga gaaaatcaca
    16381 gtatcaccag gctgtaaagt tacttgacga tgagtgccat tagcgatacg agcaagtgct
    16441 gccatagact caccttgact acctgtacac ataattaaag cttcactagc atgaagattt
    16501 ttaagttcgc ttggctcaat gaaggtacct tttggaactt taatatagcc aagttcaata
    16561 ccgttaacaa ttgctttttc catagaacga ccaaaaacgg caattttacg tcccgtttta
    16621 acagcagctt ctgcagcctg ttgcaaacgg tagatgtttg aagcgaagga agcaaaaata
    16681 atacgcccat gaataccttc gataattttt aaaatggatt ggccaacaac tttttcagag
    16741 ttagtaaagg ttgggatttc agcattagtt gagtctgata ataagcaaag gacaccttct
    16801 tcaccaagcg ctgccatacg ttgtaaatct gcaggatctc ctactggcgt gaagtcaaat
    16861 ttaaaatcac cagtacagat aatcttacct tgaggggtgt gaataacaat accgaccggt
    16921 tctggaatgg aatgagtagt cttgaagaaa gtgacgctca tgtttttaaa agttaactcg
    16981 gtgttgtgat tgatttcgta aacagttgct tcacgccaaa gaccatgttc ttccaattta
    17041 ccacggataa gagcaagtgc taaaggtcct gcgtagatag ggatattagc ctgtttgaga
    17101 agaaatggaa taccaccaat gtggtcttcg tgaccatgtg taataacaag agctttgacc
    17161 cggtctaagt tatcgacaat gtaagaatag tctggaataa cgtaatcaat tcccaaaaga
    17221 tcatcttctg ggaatttgat accggcatca acgataatga tctcgtcttg atactcaata
    17281 ccataagtat tcttcccaat ctctcccaga ccaccaatgg caaacacccc aacttcatta
    17341 ggttttaaac tgatatttgt catcttagaa ctccgttaat tcaaaaacac cggtctcttt
    17401 ttcgtaatcg aggtgtttgt cagaaagtag ctcgataaat tccacgttat aataggtgtt
    17461 atcttcaacg agacgacgag ccttgatacg gccatcgagt tcatcagtag catcaatatt
    17521 aagatagagt gctttagtac tttcacgacg tgggctttga tcctttgttt cttgatagaa
    17581 aactttataa atcatttgtt ttttttcctt tcgttatgaa tcaattatcc aaacaataat
    17641 aaataggtca aaaggtaggt gttccatttt gacggtttta tatgttgtca ttattgtgta
    17701 gtagattaat tcctgattct gtgcaattat tacaattata ccataaactt agcttttagt
    17761 aaaaaattta ggtacattta tggtatagtc taaatcaaat taagagtcag cctttttgtt
    17821 tatgattctt aatttgtgtt ataattgata ctagaacttg cagtagaaag gcataaaatg
    17881 aagacacttg catttgatac ctcaaataaa accttgtccc ttgctatact tgatgatgag
    17941 acacttctag cagatatgac ccttaacatt cagaaaaatc atagtgttag ccttatgcct
    18001 gctattgatt ttttgatgac ttgtactgat cttaaacctc aagatttaga aagaatagtg
    18061 gttgcaaaag gccctggatc ttacacaggt ttacgagtgg cagttgctac tgcaaaaacg
    18121 ttagcgtaca gtttaaatat tgcattggtc gggatttcga gtctatatgc tttggctgcg
    18181 tctacttgta aacagtatcc aaatactttg gtggtgccat tgattgatgc tagaaggcaa
    18241 aatgcgtatg taggttatta tcggcaagga aaatcagtga tgccacaagc ccatgctcca
    18301 ctagaagtta ttatagaaca attagtagaa gaagggcagc tgatttttgt tggggagact
    18361 gctccttttg ctgagcaaat tcaaaagaaa ctacctcagg cgatactact tccaaccctg
    18421 ccttctgctt acgaatgtgg tcttttgggg caaagtttgg caccagaaaa tgtagacgcc
    18481 tttgtccctc aatatctcaa gagagtggaa gctgaagaaa actggctcaa agataatgag
    18541 ataaaagatg atagtcacta cgttaagcga atctaatatg aaaacagttg aagaacaagc
    18601 aaaaaaaatt tatcagcttt tggaaatggt ttatggcacg tctccttgga ctttagaaca
    18661 agtattaatc gatatacggc gggatcaaac agattatttc ctactatatg atcatgataa
    18721 attgctaggt tttttagcta ttcaagattt agctggtgaa gttgaaatga ctcaaattgc
    18781 tatactacct agtcatcaag gacttgggct agcgtctcaa ttgatgacgc atttagatag
    18841 cattgaaagt gacatttttt tagaggttag agaatctaat catagagcac agggtttata
    18901 tcagaagttt ggttttaaac ttattggcaa aagaccagac tactatagaa atcctataga
    18961 aacagcttta ttgatgaaac gggaaggaaa acatgacaga taggtatatt ttagcagttg
    19021 agagttcttg tgatgagacc agtgtagcga ttttaaaaaa cgaaagtacc ctcttaagta
    19081 atgtcattgc aagtcaggtt gagagccaca aacgttttgg tggtgtggtg ccagaagtag
    19141 ctagtagaca ccatgtcgaa gtgattacga cttgctttga ggatgctttg caagaagctg
    19201 gcatcagtgc ttcagattta tcagcagttg cggtaactta tggccctgga cttgttggag
    19261 ccttgttagt tggattagca gctgcgaagg cttttgcttg ggcaaatcat ttacctttga
    19321 ttccggttaa tcatatggca ggtcacttaa tggcagcgcg tgaacaaaag cctttagttt
    19381 atcctttaat agcactgttg gtttcaggag gacacacaga attggtttat gtcccagagc
    19441 caggggatta tcatattatt ggcgagacgc gtgacgacgc tgttggtgag gcttatgata
    19501 aagttgggcg tgtgatgggc ttaacctatc cggcaggacg tgaaattgac cagttggctc
    19561 ataaaggtca agatacttat catttccctc gtgctatgat aacagaagac cacttagaat
    19621 tttctttttc aggactcaag tccgctttta tcaatcttca tcataatgct aagcaaaaag
    19681 gcgatgaact gattctagaa gatttgtgtg catcttttca agcagctgtt ctagatattt
    19741 tattggccaa aacaaaaaaa gctctgagca gataccctgc aaagatgctt gtagtggctg
    19801 gtggtgtagc ggcaaatcaa ggattacgag atagattagc acaagaaatt acacatatag
    19861 aagttgtgat tcctaaacta agactttgtg gagataacgc gggtatgatt gctttagcag
    19921 cagctattga gtatgacaaa cagcattttg ctaatatgag cctaaacgcg aagcctagtt
    19981 tagcttttga ccagtttcca gacagttttg taataaacta gtcacacttg ctagttagta
    20041 agatttataa tggataagaa tctccctttg aaaaaacaac ccggtatctt ggcataccgg
    20101 gttgtcatga tgataagtaa ttggcttcta tggttactaa taattcagat aattacctgc
    20161 aaaataattg tctgcctaag gtgaagtaag cttaccagct agctttagtg acgccaggaa
    20221 gctgtccttt gtgagctaag tcacggaaat tgatacggct aacaccaaac ttacgcatgt
    20281 aagcatgagg acggccgtca attttgtcgc ggttcttcaa gcggtttgga tttgaatcac
    20341 gaggcaattt gcgcagtgat tcataatcgc ccttcgcttt taagtcacga cgaaggtcag
    20401 cgtactgttc aatcagttgg agctgctttt ggtatttagc aatttttgac ttttttgcca
    20461 ttgctttctc cttcttaata tttaataacc tcagagtcgt tatcagcgcc ctgaatccac
    20521 ttaactggta tttaactggt taagtggata tataatcatt atacaataaa taaaagcaaa
    20581 agcaactaaa aaaattaact agttatcttt tttagccata atatcaaaaa actccacatc
    20641 taagtgtggg agtcaggtat taataactgt ttaaaggtgt ttaatgagtg tcgctttggc
    20701 aactttgaga ttgaactttt aagtcaaata aatcagcagg aacgtagtgt ttggttgaga
    20761 ccaagatgtc tttaccattg tagagagttt cttggattaa aataaaagca ctattttctg
    20821 atagagtata cttagttgct gtgtaattgc cagtcttggt atagccgatt tggttgcagg
    20881 aatgagaagc tttaccagat tcatagattt tctcttctaa gaaagtaaga aggggctctt
    20941 cttgattgag atttatagca tatttagaaa taacctcaat agggataaaa gataaactat
    21001 aagcaatagc tttattttgg ctatgatacc agcgatcaac aataatcaca acaggagttt
    21061 cttgttttag ggatgctgta atggcaacag tgggcacttc aatccgatat tccaattcaa
    21121 cctttgtgat gtcagatgat aggcttgcat atatggggtg ttgaagatat tcgaaacctt
    21181 ggtgatattt agtttcagga gtcttgagta taaaatttcc ctttcctctg atatttttta
    21241 tgagatgatc ctcttggagg agggctaggg atttacgtag tgtcatccga ctgacattga
    21301 gctgtctagc aagttcaggt tctgatggca gttgcatgcc aggactatat gtaccatctt
    21361 gaataagttg aaaaatagta ttatatactt gtacatgttt aagttttttg agttttttgg
    21421 ttaagtcgtt agtagacata tcacttacct cattaataat ctttagtgga gtttaacttg
    21481 tctaaaaact attttagcac taacagtatt ccttgattag aattgtaccc atctatagtg
    21541 ttagtatctc acactcaggt actttttgtc aaaaaagaga tgaaaaacac cttaaaaaag
    21601 cgtttacaaa atagggtgtg tgtgatacta tacaagtgta tcaaaaatta gacaagtaaa
    21661 atacaggagg tttttaacat gcaaaattat tcaggtgaag tcggattgca atatcaccta
    21721 cagattcgtc caggtgatgt aggtcgctat gttatcatgc caggtgatcc aaaacgttgt
    21781 gcaaaaattg cagaacattt tgataatgca gttcttgtag cagacagccg tgaatatgtt
    21841 acgtatacag gtacattaaa cggtgaaaaa gtcagtgtta cttcaactgg tattggtggt
    21901 ccatcagcgt ctattgcgat ggaagaattg aaactctgtg gcgctgatac ctttatccgt
    21961 gttggaacat gtggtggtat tgaccttgat gttaaaggcg gagacatcgt tattgcaact
    22021 ggagctatcc gtatggaagg taccagtaaa gaatatgctc cgattgaatt tcctgctgta
    22081 gctgacttgg aagtgacaaa tgctctagtt aatgcagcta aaaagcttgg ttatacaagt
    22141 cacgcaggag tagttcaatg taaagatgca ttttacgggc aacatgagcc agaacgtatg
    22201 ccagttagtt atgaattatt aaataaatgg gaagcatgga aacgtctggg aacaaaagcg
    22261 tctgaaatgg aatctgcagc tctttttgta gcagcaagtc atctaggagt tcgttgtgga
    22321 tcagatttcc ttgttgttgg taatcaagaa cgcaacgctt tgggtatgga taacccaatg
    22381 gctcatgata cagaagcagc tattcaagtt gctgttgaag cactccgcac acttattgaa
    22441 aatgataaat cacaataagc gaggttttgg aagtcatcat gcaatttatt tatagtatta
    22501 ttggtatttt attgatatta ggaattgtgt atgcaatttc tttcaatcgt aagagtgttt
    22561 ctctaagttt aattggaaaa gctcttatcg ttcaattcat tattgcgcta atcttagtac
    22621 gtatcccact aggccaacaa attgttagtg ttgtttcaac tggagttact agcgtaatca
    22681 actgtggtca agctggttta aattttgtgt ttgggtcatt agcagatagt ggcgcaaaaa
    22741 ctggttttat tttcgctatt caaacgcttg gtaatattgt tttcttatct gccctagtta
    22801 gtctacttta ttatgtggga atccttggat ttgtagtaaa atggataggt aagggcgttg
    22861 gtaaaattat gaaatcctca gaggttgaga gttttgttgc cgtagctaat atgtttcttg
    22921 gtcaaacaga cagtccaatt ttggttagca aatacctagg tcgtatgact gatagtgaga
    22981 taatggttgt gttggtatca ggtatgggaa gtatgtcagt ttctattctt ggtggctata
    23041 ttgcattagg cattccaatg gaatatctct tgattgcttc aacaatggtt cctattggca
    23101 gtattctcat tgctaaaatc ttattgcctc aaacagaacc tgttcaaaaa attgatgaca
    23161 ttaagatgga taataaaggt aataacgcca atgtgattga tgcaatcgct gagggtgcaa
    23221 gcacaggtgc acaaatggct ttctcaattg gggctagttt gattgccttt gttggtttag
    23281 tttctttgat taatatgatg ttaagtggat tgggaatccg cttagaacaa atcttttcat
    23341 atgtttttgc tccatttggt tttcttatgg gatttgacca caaaaacatt cttctagaag
    23401 gaaaccttct tggaagtaag ttgattttaa atgagtttgt ttcgttccaa caattgggtc
    23461 acctaatcaa atctttagat tatcgtacag cattggtagc aactatttca ctctgtggtt
    23521 ttgctaattt atcaagttta ggtatttgtg tttcaggtat tgctgttctt tgcccggaga
    23581 aacgtagcac cctagctcga cttgttttcc gtgcaatgat tggtggtatt gctgtaagta
    23641 tgcttagcgc ctttatcgtc ggtattgtaa ctctattcta aacgttgaca aaagaaagaa
    23701 ggatagtaac gtggaagtaa aagatatttt aaaaacggta gaccatactt tgctagcaac
    23761 aacagcaacg tggccagaaa tccaaacaat tttagatgat gccatggctt atgaaacagc
    23821 ttcagcatgt attccagctt cttacgtcaa aaaagcagca gaatacgttt caggtaaatt
    23881 agctatttgt actgttattg ggttcccaaa tggctatagt acaactgcag cgaaggtttt
    23941 tgaatgtgaa gatgctattc aaaatggtgc tgatgaaatt gacatggtca ttaatttgac
    24001 agacgttaaa aatggggatt ttgatactgt tgaagaagaa attcgtcaaa tcaaagctaa
    24061 atgtcaagac catatcttaa aagttatcgt tgagacatgt caattaacta aagaagaact
    24121 tatcgaactt tgtggagttg tcacacgttc aggtgcagac tttattaaaa cctctactgg
    24181 tttttcgaca gcaggtgcta catttgaaga tgttgaagtg atggcaaaat atgtcggcga
    24241 aggtgttaaa attaaggcag caggtggaat ctcatcattg gaagatgctg aaacatttat
    24301 tgctttagga gcttcacgct tgggtactag ccgtatcatc aagattgtta agaacgaagc
    24361 tataaaaacc gatagctatt aacttaatca taccgattta gcttgagact aaaacaaaaa
    24421 actcatgtaa taggagttaa aaagcctact atcttagtga taataggctt ttttagctat
    24481 gaagacttcc ctgcgatcca tcctgaacaa agagctgagg taatattaaa ccctccggtg
    24541 tgagcattaa tatctaagac ttccccagca aaatacagcc caggaacttt cttactttcc
    24601 aaggttttag gattaatttc ttttaaatcc acgcctcctt tggtcacaaa agatttagcc
    24661 aaagacattt ttcctgtaat gggtatctgg aggtgtttga gtttatcaag caattccttc
    24721 tcttgttttg gagagagttg tttgaccttt tcgggataat cttctgataa aaaatcagct
    24781 actcgttcag gcagtaggcc tttaagagca tttttgatgt ttttatcacg ttgatcactc
    24841 agataagcgg ttaagtcatc tgttgataag tgtggcaaaa aatctagctc tgctatttcc
    24901 ccacctttca caaaagaaga caaacggaga gctgcaggac ctgaaaggcc aaagtgagtg
    24961 aagagtaagt cgtgtgtgat gacgtgtttg tcataactaa gagtaacgtc gtctagggaa
    25021 ataccttgca agactttgtg aggaaaatca gttaacagag gactctcagc agcttctaaa
    25081 tcagtcacgg tcagtttaaa atgccgtgca atatcatgtc caaaacctgt tgaacccgtt
    25141 gaaggatagg ccttgcctcc tgtggtcaca atcaatttat gacagctaaa agtctggtct
    25201 gcagatttga ggtagaagag gtcatcttgt tttttaacgg aaaccacttc agtgctggtt
    25261 agtaccttgc ctccaagagc cttgattttt ttctccagag catcaatgat tgtcctagat
    25321 ttatctgttg tggggaacat ccgtccatgg tcttcttctt tgagtttgac gccgttttcc
    25381 tcgaaaaagg caatgatgtc atggttgtca aactgggaaa aaacactata taaaaagcgg
    25441 ccatttccag gaatgcctgc cataaggtca tcaaggttac cactattagt gacattacag
    25501 cgtccaccac cggttcccgc taatttttta cctaagcggc gatttttttc aattaagaga
    25561 gttttatagc catagtagct gctggagatg gcggccatca tgccagcagg gccacctcca
    25621 ataataatcg tatcgtattg agtcatagcc ttattttatc ataaaagcgg ggggtttaat
    25681 agtcaggtaa atgaactaac ctggcatacc gttttctatt catctaacaa tgttttcaag
    25741 aaataattga tgcctttagc acgactgaaa tgaggtcaaa ttgacagaat actcctatta
    25801 tggtactatt tttaataaga atattcagtg atagtgataa agaggaaaat atgggaattg
    25861 aaaagacagt tagtgaacta gctgatattt tgggagtgag tcgtcaggcc gtcaataatc
    25921 gtgttaagtc tttacccgaa gaagatcttg acaaaaatga aaaaggcgtt actgtggtta
    25981 agcgcagtgg tcttgttaag ctggaagaga tctataaaaa aacaattttt gacgatgagc
    26041 ctatcagtga agaaacgaaa caacgtgagc ttttagagat tcttgtggat gagaagaaca
    26101 ctgaaatcac gcgcctttat gagcaactca aagccaaaga tgctcaactt gcctcaaaag
    26161 atgagcaaat gcgtgttaaa gacgtgcaga tcgctgaaaa agataaacaa ttggatcagc
    26221 agcaacaatt aactgctaaa gccatggctg ataaagaaac cctaaaatta gaattggaag
    26281 aagctaaagc agaagccaac caagctcgct tacaagtcga agaagttcag gctgaagttg
    26341 gccctaaaaa aggctttttc acccgcttgt ttgcgaaata atcacaaaaa atgacgaagg
    26401 ccctatcagg gtcttttttg gaggaaaaca tgaaacactt agatacttat attgcctttg
    26461 atttagaatt taacactgtc aatgacgtta gtcatgtcat tcaagtatca gcagtcaaat
    26521 atgaccacca taaagaagtt gacagctttg acacctatgt ttacacagat gttcccttac
    26581 agagctttat caatggccta acaggaatca catctaacaa gattgctgcg gcaccaaaag
    26641 tagaggaggt tatggctgcg tttaaaaatt ttgtgggaga gttaccactg attggttata
    26701 atgctcaaaa atctgacctg cctattctag ctgaaaatgg cttggattta agggaccaat
    26761 accagattga cctctttgat gaagcttacg atagacgtag cgcagactta aatgggattg
    26821 ctaaccttcg tctacaaaca gtcgcaacct ttttaggcat taagggtcga ggtcataata
    26881 gcttagaaga tgccagaatg acagcagcaa tctataagtc ttttttagaa acagatacaa
    26941 acaaagcata tctcagccag caagaagagg taaccacaga taaccctttc gcagctttgg
    27001 gagacttttt cgactaaagg ctctgctatt tttgaaaaat aaaaaaatcc ctagggttag
    27061 cccttgctta taacgctagg tcatgtgata agctagggac atatcaagag ggagtcgagt
    27121 gcacacgaat cgtttttgat cactaaatga tagaaagaag gtgtggaaat gtctcaattc
    27181 tattcgactg gagaactggc caacttagct ggcgtttcta ttcgaactgt ccaatactat
    27241 gaccaacgtg ggattttgat tccgacagca ttgacagcag gtggacggcg actatatacg
    27301 gattctgatt tagaacaatt acgcatgatt tgctttctgc gcgatttggg attttccatt
    27361 gagcagattc gcaaagtctt agcagaggaa aatgcagccc aggtgctgga attgcttcta
    27421 gttgatcata ttgcgactgc caaggaagac ttggccgcaa aagagcaaca agttgacata
    27481 gctgtcaaga tacttgacag acttcgaaaa caagaccccc agtcactaga ttttctgatg
    27541 gacatttcgc ttagcatgaa aaatcaaaaa gcttggaaaa aattacaatg gaagatgggt
    27601 atcagtatat ttttagccat tgtttcatat gtaggactta ttttactatc taattatctg
    27661 caaaaatttt ggttagttta tgttgctatg ggaatatttc taccaggttt cagttggttg
    27721 gtaatttatt ttaggaagca atttatttat ctctgtcccc attgtcatca gaaatttgaa
    27781 cctagcttta atgaatttgc ccttgcaggc catacaccac gaaccagaaa gttaacctgc
    27841 tcccattgcc atcaaaaatc gtattgcttg gaactggcaa aagaggacac tgtctcaaga
    27901 ggataagcac taactcataa aaaaactagc ctgagatcct tctcaagcta gtttttgttt
    27961 ttttagaatg gtaatttacc agcaaaagcg cctaatgttt tcttagtcgc ttcatcaatt
    28021 tgcgtaaggg catcattaat ggcttgagtg gtcatgtctt gaagcgtttc tacatcttca
    28081 ggatctacaa cagcttcttt gaagtcaatc ccaaccaatt ttttatcacc cgtgaaagtg
    28141 gcagtgacaa ggtcttgagc tgattttcca gtaaattgca tagcagccaa atccgcctgc
    28201 ttttgctcca tttgtttctg aagcttttgt gcttgcttca tcatgttttg catgttcatc
    28261 ataagactat actctcctta atttaatact aactattata acatataaaa agaggcaata
    28321 aaaacaatgt cagttagtag actagcctgt atcagtaaga aagtcgctat ggataagatg
    28381 gattagcagc aagggtctgg ctaatgacag aataagtcta gaaatggatt atttttgtca
    28441 tagactagtc cgacttagtg gttatctaat tcacgatctt ctttagcttg ttgttctaaa
    28501 agaatttgtg cgaagtcaac ccacttagtg tagttatttt cggataattg ttctgaaatc
    28561 atcgctaact ctcgttgtgg caactgataa gagactccct tactttctaa ggttaaatct
    28621 tcttccatta gggcggttaa actaacatca aagatagcag caatatcact taatagacca
    28681 gactttggag tatattttcc tttttcccac tcactgattg aagaagagct ttttcgtcct
    28741 aggagattag caagttctaa ttgttccatg ttctttttta atctaaggta ctttaaatta
    28801 cttgcaaaaa aagttttctt ggcggacata tttttctcct tttctttata taaaatatga
    28861 taccataaaa gaggttaaag caaaaggtat cgtaaaagat ggtctaaaaa ctctctaaaa
    28921 acttgacttc ataaaaaccg aagtttataa taaacttaac atgattaact aatctcattt
    28981 tagtgtggag ggaaaattgt gaccaaatca actttaaaag agttaagaac aacaaagaaa
    29041 atgacacagc aggaattgtc tcacttaaca ggcatttctg tcagaacgat tgctaggtat
    29101 gaaaaagata ctaaaaagct aaggcgagca aaatatgaga agctaaaagg tattgcacaa
    29161 gcattagcgg tttccgttga tgatattttt ttaggaatag attcggaatt tatgaactaa
    29221 gacgatcgtt ttctgtttgc tattagtaag accaattaac aaaaaagtgc ttagttatat
    29281 tgctaagcac ttttatttgt taatttgatg ctattggcac aatcaattga gactgactga
    29341 gatcgatagt taaagcatag ttgctgttgt cacggacagt atgttcaaaa tcggttgtgt
    29401 ataagatgac tcgaagggta tctccttttt ctagatgata gatagttggt tgcaaaggaa
    29461 gtctaacagt catccattta ttattaggaa tagtttcaat tgacgacaaa ttattgcgat
    29521 tttgtaaatt catgaaacct ttcgaaataa cacggtaaga atcttctcta aatggtagct
    29581 ctttaagggg ttctctagag aaattttgcc cattgtcaat acttgattgg gtgagagcaa
    29641 taggaagatc gcccaagcgt tttttcttac catagtcaag gatttgtgca gatagaagtc
    29701 ctttattttc acttgatttc acttttaact gtaggacaat ttcaccgtta ataggtaagt
    29761 cctcttccag taaaatatca atcagggctt gattggcctt gcctttaaag agagctgctt
    29821 tggacgcacg gaaatcttta ccgtaagctt taaattcatc ttcaccatag tgattatcaa
    29881 ttaaaagcag ttcctgaccg aggtcaagct ctttgatggt tgaggttcca aagactttac
    29941 gctcctgcca gttttgtggg caggtgttat cctgccagat catttcagga aggctaaaat
    30001 cattagctag gcctaagagt ttttgacata aaagggcatt catgctttcg cgaaaatcaa
    30061 tggattgcca attatgcatg taaacatgct caccttgatg taaaaagagg tgcttgttga
    30121 ttgtggaagg aagcgcattg aaaatctcat agacctgtct tggtttgaca ttccagtctt
    30181 gtaagccatg agtataaacg acatcacact ttatttgatg agcatttttt aagtaattgc
    30241 ggtcgtgcca aaattgatta taatcaccag attgtcggtc taacgcctga gactgttggt
    30301 ttagcaattc ttgataatga tcattatggc gaagataatc gccagccaat aaattacgcg
    30361 agtaggtgag ctcagttagg acatctagat cctctccagg atagccacca ggactacaaa
    30421 ctaagccatt ttcgcggtag tagttgtacc aagaagaaat agctgattct gcaataatca
    30481 tggctaatcc gtctacgcct gtagtggcta aaccagtaga catagttccc aagtaggact
    30541 ttccagtcgt acaaactaga ccatttgccc aatcagctct cacttggtgg gtttttgagt
    30601 ggctggtgta ggctgtggct cttccattta gccaatcaat aacggcttta aagctttcaa
    30661 tttgagcata gtctccactc gtcataaagc cagtggaacc agcagttccc acacctgaaa
    30721 catagatatt agcaaaccca cgtgcgagga aataatcgtt gaggctatag gagttgatgt
    30781 agctaaagct ttctagattt tgacctatag gaagtttgca aggctgtgtc tcaagaggaa
    30841 taaaatccct gtcttcgaca gtgatttgtc tccttttttt aacgactaac tctttttcca
    30901 tacgataaag ttttttgtcg ttggctactt cattgatacc ttgatggtat ggactaggtg
    30961 tcatcaaagt tggtaatggt ttttgagatt gaggacgaat gatattaacc ttaataagat
    31021 caagttggcc atcttgatcg gtatcaagag gtgcttcaac gtaaacaact tctcgaatca
    31081 gctgtgacgt gtcaaaggta gctaatgatt taccgttgaa gaaatggtaa tcattgtcca
    31141 tagtaagcat tccttggctg accaaatcat caatcagggt catgccagac ttacaacggg
    31201 tcgctaataa gtggtggata tgcttgataa tttctgtttc gtcaaagtgg ataggaaagg
    31261 ccagtttatc aaggaaagca tcggcctctg taaaatcaac tccaggaata aagccaagga
    31321 cttgcagggc cagtaaatca aaaacctctt tggataactc ttgctctgat tgaaaaaagc
    31381 tcaatgcgtc catgtcattt ttggcgatta gatgacttaa aggataatca ctatcagggt
    31441 agtggaagaa gagtttacgt aaaaagcttt ctaggttagc tttggcagtt ttttgcagat
    31501 ccagatcaaa gcccaattct tttaattctt cagccgctct ttctagacta gttggaatat
    31561 aggaaaattg attatagcgc ataggagttt cctttctagt ggcttagaaa acgatattat
    31621 cttttaattt atcaagattg gtaatgataa tatgttgttt atcaatgcga atgatgtttt
    31681 tttctttcaa ctgttttaga atacggctga cgctgctagc ggtagaaatg ccacagaatt
    31741 taccgagttc ctcattagta atgacaaaat taattaaaat ttgaccattt ggtagttctt
    31801 cgccaaatag ggtcattaag tcatatattt gagtagaaat ggcaccgata cggccgttag
    31861 ttaacataca ttgcattttt ttcatggatt tttgaagacg attatggtaa aagtccttca
    31921 catatccttg tagttcaata tcttcgttta tatcttttaa aaatgctgag cggcgaacct
    31981 tgtaaaaact agctttctct gactcgatgc gaacattata aggctctccc atatcttttg
    32041 aataaccagt gttgagaaca gaagtaattt caagtcctgt aacatatctc aaattaaatt
    32101 ccattccata ttttgaaaga acactctgtt tcacaatgcc gtcttttaaa atataagtga
    32161 agtcctcttc gagactttca aaagtcaagt acttgtgata acttttttca atgataggaa
    32221 agttatgatt atcaatatac cgttgcaaaa tgtgatgatt cacgcttttc tccaaatatt
    32281 cttttaccaa tgtataattt gtatcttcaa gagaaattaa catttgctaa tgccctttga
    32341 tagtaacact tatattatac cattgaaata tattatattc tgcttttttt gtaaaatgtt
    32401 tatgaaaata gagaggagtt cattttcaat ggaagaaaaa ttgtttaaca aacattttgt
    32461 ggccatcaca gtaataaact ttatagtcta tatggtctac tatcttttta cggttattat
    32521 tgcctttgtg gcgactagag agctaggagc tcagactagc caggcaggat tggcaacagg
    32581 gatttatatt ctagggactt tattagcccg tttgattttt ggaaagcagt tggaggtgtt
    32641 tggtcgtcgt ttggttttaa gaggaggtgc tattttttac ctcttaacga ccttagccta
    32701 cttttatatg ccaacgatta gcatgatgta cttggttcgt ttcttgaacg gatttggtta
    32761 tggtgtagta tcaactgcga caaataccat tgtaacagcc tatattccag ctcgtaaaag
    32821 aggagaagga attaattttt atggcttgtc aaccagtcta gcagcagcta ttggcccttt
    32881 tgtaggaacc tttatgttgg ataatcttca tattgatttt cgcatggtta ttgtcttatg
    32941 tagcgtcttg attgggtgtg tggttgttgg tgcttttgct tttccagtca aaaacatgtc
    33001 tttaaatgcg gagcaattgg ctaaaaccaa atcgtggact gtggatagtt tcattgagaa
    33061 aaaagccttg tttatcactg caatcgcctt tttaatgggg attgcctatg cttcagtttt
    33121 aggatttcaa aaattataca cgtctgaaat tcatttgaca acagtaggcg cttatttctt
    33181 tgtggtctat gccttaatca ttaccatcac tcgtccagca atgggacgtt taatggatgc
    33241 taaaggcgat aaatgggtct tgtacccaag ttatcttttt ttagcaatgg gactcttctt
    33301 gctaggtagt gtatcgtctg gaggaagcta tttgctttca ggagccttga ttggctttgg
    33361 ttatggcact tttatgtctt gtggtcaagc cgcctccata caaggagtgg atgagcaccg
    33421 ctttaatacg gctatgtcta cttatatgat tggtcttgat ttaggtttag gtgctgggcc
    33481 ttacctcttg ggcttgatta aagatttagc acttggtagt ggtgtggctt cctttaggca
    33541 cttattctgg ttagctgctg tgattccact gatttgtacc ttgctttact tgttaaaaac
    33601 aaagacaaga caggttgtgt cttaaggaca agaaaaggag tttagtctta tacttaaata
    33661 caaacgtatc ttaacagctg tagatagttc ctatgagccg ggattagctg tgggtaaagc
    33721 cattactata gcgctttgaa ataattctag tttgctattc gtacatgtca ataaaaatga
    33781 ctcgcaaaag ccattcgtga aagagaaaat gtggccttat ttttagtagg tgcaattgga
    33841 ttaagcacgt ttagatacat gacaattgga ttaacatcag aatataggct ttgtcatgtt
    33901 aagattgatc tgttgatagt gagagctaat aaaaaatgtt ataatactat gaaataaagt
    33961 gataaagata aggcctttta tggtcctttt ctttaccgct tttattattg tgtg
//
LOCUS       DAUDYX010000017        31637 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.017, whole genome shotgun sequence.
ACCESSION   DAUDYX010000017 DAUDYX010000000
VERSION     DAUDYX010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31637
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.017"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            18..98
                     /locus_tag="VHD02_000783"
     tRNA            18..98
                     /locus_tag="VHD02_000783"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52..54,aa:Tyr,seq:gta)
     gene            105..175
                     /locus_tag="VHD02_000784"
     tRNA            105..175
                     /locus_tag="VHD02_000784"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:137..139,aa:Trp,seq:cca)
     gene            187..259
                     /locus_tag="VHD02_000785"
     tRNA            187..259
                     /locus_tag="VHD02_000785"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:220..222,aa:His,seq:gtg)
     gene            268..339
                     /locus_tag="VHD02_000786"
     tRNA            268..339
                     /locus_tag="VHD02_000786"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Gln,seq:ttg)
     gene            359..442
                     /locus_tag="VHD02_000787"
     tRNA            359..442
                     /locus_tag="VHD02_000787"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:393..395,aa:Leu,seq:caa)
     gene            702..887
                     /locus_tag="VHD02_000788"
     CDS             702..887
                     /locus_tag="VHD02_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497411.1"
                     /translation="MSFEIFTLIIGWKLVGYRVMECGSSFKQGDYRLVVHICLLNVRD
                     EMLSQQGHEDKADWPEP"
     gene            1525..1833
                     /locus_tag="VHD02_000789"
     CDS             1525..1833
                     /locus_tag="VHD02_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEQ5497412.1"
                     /translation="MKHRFQVKRGVSVYLEKRIPMCAGMGGGSSDAVTIRALNQLWLL
                     TLSRKDMMDIGIPIGSDVSYCLLSGCAQVTGKGEVVCRILGLLSSWVVLVKPDFGIST
                     "
     gene            1888..2100
                     /locus_tag="VHD02_000790"
     CDS             1888..2100
                     /locus_tag="VHD02_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEQ5497413.1"
                     /translation="MVAAIEAGNYNDGILTEMNNLLEDIFIAKRPFIQKIKEKTLQAG
                     AATTLMTGSGPTVFALCQTEKQTIIV"
     gene            2210..2653
                     /locus_tag="VHD02_000791"
     CDS             2210..2653
                     /locus_tag="VHD02_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEQ5497414.1"
                     /translation="MGTLEKKLDNLVNTILLKAENQHELLFGACQSDVKLTNTQEHIL
                     MLLSQQRLTNTDLAKALNISQAAVTKAIKSLVKQDMLAGTKDTVDARVTYFELTELAK
                     PIASEHTHHHDETLNVYNRLLQKFSAKELEIVDKFVTVFAEELEG"
     gene            2657..3376
                     /locus_tag="VHD02_000792"
     CDS             2657..3376
                     /locus_tag="VHD02_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEQ5497415.1"
                     /translation="MRYISVKNLSFQYESEPVLEGITYHLDSGEFVTMTGENGAAKST
                     LIKATLGILQPKAGRVTIAKKNKDGKQLRIAYLPQQVASFNAGFPSTVYEFVKSGRYP
                     RSGWFRHLNKHDEEHVQASLEAVGMWENRHKRIGSLSGGQKQRVVIARMFASDPDIFV
                     LDEPTTGMDSGTTDTFYELMHHSAHQHGKSVLMITHDPEEVKAYADRNIHLVRNQKLP
                     WRCFNIHEVETDDEKGGHGHA"
     gene            3369..4184
                     /locus_tag="VHD02_000793"
     CDS             3369..4184
                     /locus_tag="VHD02_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEQ5497416.1"
                     /translation="MLDILSYDFMQRAVMAVVAISIFAPILGIFLILRRQSLMSDTLS
                     HVSLAGVALGVVLGISPTITTIIVVVLAAILLEYLRVVYKHYMEISTAILMSLGLALS
                     LIIMSKSHSSSSMSLEQYLFGSIITISMEQVVALFAIAAIILILTVLFIRPMYILTFD
                     EDTAFVDGLPVRLMSVLFNIVTGVAIALTIPAAGALLVSTIMVLPASIAMRLGKNFKT
                     VILLGIVIGFSGMLSGIFLSYFFETPASATITMIFISIFLLVSLGGMLKKRLF"
     gene            complement(4224..4607)
                     /locus_tag="VHD02_000794"
     CDS             complement(4224..4607)
                     /locus_tag="VHD02_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEQ5497417.1"
                     /translation="MTECIFCHQLKANQLLTQSQYFKVVFDIDPIQKGHLLLISKDHY
                     TSLTQLPKEVRYDLIDLQAALVAKLEQHLPISGVTSVSNDKELMDEGTHFHLHLIPRL
                     TNDSFWESITINQENWDLTPFLKHL"
     gene            complement(4658..5914)
                     /locus_tag="VHD02_000795"
     CDS             complement(4658..5914)
                     /locus_tag="VHD02_000795"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEQ5497418.1"
                     /translation="MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKETVLEWSDKIK
                     GQLSTFLDFENGDNKAELVNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIET
                     GISYTEFAYQIMQGYDFYELNDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDE
                     IAEIETQFNAARHERLAQKTLAREVVTLVHGEEAYKQALNITEQLFAGNIKNLSANEL
                     KQGLSNVPNYHVQSEDSLNLVDMLVTAGISPSKRQAREDVQNGAIYINGDRVQDLDYQ
                     LSNDDKIDDQLTVIRRGKKKYAVLTY"
     gene            6006..8318
                     /locus_tag="VHD02_000796"
     CDS             6006..8318
                     /locus_tag="VHD02_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ5497419.1"
                     /translation="MRYFMVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVI
                     FLFGMMGFGMAFGYLASQIESVKVPSKESLVKQVESLTMISQMNYSDNSLISTLDTDL
                     LRTPVANDAISENIKKAIVSTEDEHFQEHKGVVPKAVFRATLASVLGFGEASGGSTLT
                     QQLVKQQVLGDDPTFKRKSKEIVYALALERYMSKDNILCDYLNVSPFGRNNKGQNIAG
                     VEEAARGIFGVSAKDLTVPQAAFLAGLPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQN
                     VLYNMYRTGVLSKKEYEDYKAYPIQKDFIQPGSAIVNNHDYLYYTVLADAKKAMYSYL
                     IKRDKVSSRDLKNDETKAAYEERALTELQQGGYTITTTINKPIYNAMQTAAAQFGGLL
                     DDGTGTVQMGNVLTDNATGAVLGFVGGRDYALNQNNHAFNTVRSPGSSIKPIIAYGPA
                     IDQGLMGSASVLSNYPTTYSSGQKIMHADSEGTAMMPLQEALNTSWNIPAFWTQKLLR
                     EKGVDVENYMTKMGYKIADYSIESLPLGGGIEVSVAQQTNAYQMLSNNGLYQKQYIVD
                     KITASDGTVVYKHENKPIRIFSAATATILQELLRGPITSGATTTFKNRLAAINPWLAN
                     ADWIGKTGTTENYTDVWLVLSTPKVTLGGWAGHDDNTSLAPLTGYNNNSNYLAYLANA
                     INQADPNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVNVNGHTFSVGGEMTTSLWSQK
                     GPGAMTYRFAIGGTDADYQKAWGNFGFRKN"
     gene            8582..12148
                     /gene="rpoB"
                     /locus_tag="VHD02_000797"
     CDS             8582..12148
                     /gene="rpoB"
                     /locus_tag="VHD02_000797"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEQ5497420.1"
                     /translation="MAGHEVRYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDSG
                     LKEVFEDVLPISNFTDTMELEFVGYEFKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDSKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGESDLVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLIARFFDARRYDLAAVG
                     RYKVNKKLNIKTRLLNQIIAENLVDAETGEILVEAGTEMTRSVIESIEEHLDGDLNKF
                     VYTPNDYAVVTEPVVLQKFKVVSPIDPDRVVTIVGNANPDDKVRALTPADILAEMSYF
                     LNLAEGLGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNDVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRATGRVTNEIV
                     WLTADEEDEYTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFSASVVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAQH
                     NGKVVFSDAEKVEIRRQDGSLDVYHITKFRRSNSGTAYNQRTLVKVGDIVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEEALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGIVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWDTVREAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDIMHVDDLEKAREKQAQETQEVSETTDEK"
     gene            12239..15862
                     /gene="rpoC"
                     /locus_tag="VHD02_000798"
     CDS             12239..15862
                     /gene="rpoC"
                     /locus_tag="VHD02_000798"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEQ5497421.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEPKSLLTEREYREKLQ
                     EYGHGSFVAKMGAEAIQDLLKRVDLAAEIAELKEELKSASGQKRIKAVRRLDVLDAFN
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVAKEYAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDKDEAVMAYRNGYAHLHSRVGIAVDSMPNKPWKDSQRHKIMVTTVGKI
                     LFNDIMPEDLPYLQEPNNANLTEGTPDKYFLEPGQDIQEVIDGLDINVPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     EINKAFRRGLMTDDDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLLIRAITDGKEVTETLEERLQGRYTRKSVKHP
                     ETGEVLIGADQLITEDMARKIVDAGVEEVTIRSVFTCATRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGNVVEIEEDASTRTKKVYVQGKTGMGEYVVPFTARMKVEVGDEVNRGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPQAMNEIEVIDHTEVSA
                     EAE"
     gene            16014..16379
                     /locus_tag="VHD02_000799"
     CDS             16014..16379
                     /locus_tag="VHD02_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEQ5497422.1"
                     /translation="MYQVIKMYGDWEPWWFIDGWQDDIIDEQQFSDWQEALDYFNQEW
                     QRMKAIFPSYHSQKNLLATFWEKEDKRWCEDCDEDLQQFHSLLLLKNKDIVPSNNYIP
                     EFEQRNDSPQVAYLCKLNL"
     gene            16472..17410
                     /gene="tadA"
                     /locus_tag="VHD02_000800"
     CDS             16472..17410
                     /gene="tadA"
                     /locus_tag="VHD02_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEQ5497423.1"
                     /translation="MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVD
                     VYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYKLSKDKQLSLRLSSVGDYRGQES
                     LVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISSYHQEA
                     QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAV
                     IRASLTGAMVFSTVHAKSISGVYARLLELGITKAELSNCLALIAYQRLLNGGALIDST
                     QNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII"
     gene            17394..18380
                     /locus_tag="VHD02_000801"
     CDS             17394..18380
                     /locus_tag="VHD02_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEQ5497424.1"
                     /translation="MKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYV
                     LKMEESLLKGQGLADMLSGLGFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRR
                     KTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLNHFPAFFIGFCSGLILL
                     FGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWSTLIGQGLDLMTILDI
                     MAIEKSSLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGVELE
                     IYAQESWEQFFSQLYQVTQLIQPAIFLVVAVTIVMIYAAILLPIYQNMGGIF"
     gene            18382..18708
                     /locus_tag="VHD02_000802"
     CDS             18382..18708
                     /locus_tag="VHD02_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEQ5497425.1"
                     /translation="MINQWNNLRHKKLKGFTLLEMLLVILVISVLMLLFVPNLSKQKD
                     RVTETGNAAVVKLVENQAELYELSQGSKPSLSQLKADGSITEKQEKAYQDYYDKHKNE
                     KARLSN"
     gene            18683..19111
                     /locus_tag="VHD02_000803"
     CDS             18683..19111
                     /locus_tag="VHD02_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEQ5497426.1"
                     /translation="MKKPVLAIKAFTLLETLLSLSVMSFIILGLSVPVTKSYQKVEEH
                     LFFSHFEHLYRHQQKLAILQQKQRVLDISSTKIVTEGNSLTVPKSITVNHPYRLVIDQ
                     MGGNHSLAKIIFDMTDRRFKYQFYLGSGNYQKTSQSLHSP"
     gene            19116..19352
                     /locus_tag="VHD02_000804"
     CDS             19116..19352
                     /locus_tag="VHD02_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HEQ5497427.1"
                     /translation="MIATGILFSIVILVLSSLQQSQAALTYYRKQQEKLNLALMAVQT
                     RTKEMTLNGCHITILRTDRYISIHDDEGEVMKIE"
     gene            19345..19779
                     /locus_tag="VHD02_000805"
     CDS             19345..19779
                     /locus_tag="VHD02_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEQ5497428.1"
                     /translation="MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYL
                     ARHDQDNWLLFSHQLREELSGARFCKVADNKLYVEKGKKVLAFGQFKSHDFRKSASNG
                     KGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD"
     gene            19763..20089
                     /locus_tag="VHD02_000806"
     CDS             19763..20089
                     /locus_tag="VHD02_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HEQ5497429.1"
                     /translation="MPFKTNLKAGVLLYAMFMASIFLLVLQVYLSQVTALHKEYQAQT
                     DYVKARLIAEIVYQDHRYKASNPVFFKGGQVICRERKERWMLIVKLDQQRQYQFEYLK
                     PKQYSQ"
     gene            20187..21140
                     /locus_tag="VHD02_000807"
     CDS             20187..21140
                     /locus_tag="VHD02_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ5497430.1"
                     /translation="MTFEKIEEAYQLLLENCQLIENDLKTHIYDAIVEQNSFYLGAEG
                     ASPQVAQNSDKLKALCLTKEEWRKAYQFLFIKAAQTEQLQANHQFTPDAIGFILLYLL
                     EQLSDKDSLEVLEIGSGTGNLAQTLLNNTSKSLDYVGIELDDLLIDLSASIAEIMDSS
                     AHFIQEDAVRPQLLKESDIVISDLPVGYYPNDDIAKRYKVASSDKHTYAHHLLMEQSL
                     KYLKKDGFAIFLAPVNLLTSPQSQLLKQWLKDYAQVVALITLPDSIFGHPSNAKSIIV
                     LQKQTDHPMETFVYPIRDLKLAENIHDFMENFKKWKLSNVN"
     gene            21199..22395
                     /locus_tag="VHD02_000808"
     CDS             21199..22395
                     /locus_tag="VHD02_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEQ5497431.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEEEVLAQGIIERIGLKDSISTV
                     KYDGKKEEQILDIHDHTEAVKILLNDLIHFGIIAAYDEITGVGHRVVAGGELFKESVV
                     VNDKVLEQIEELSVLAPLHNPGAAAGIRAFRDILPDITSVCVFDTSFHTSMAKHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHKYVAQEAAKMLGRPLEELKLITAHIGNGVSITANYH
                     GKSVDTSMGFTPLAGPMMGTRSGDIDPAIIPYLIEQDPELKDAADVVNMLNKKSGLSG
                     VSGISSDMRDIEAGLQEDNPDAVLAYNIFIDRIKKCIGQYFAVLNGADALVFTAGMGE
                     NAPLMRQDVIGGLTWFGMDIDPEKNVFGYRGDISTPESKVKVLVISTDEELCIARDVE
                     RLKNTK"
     gene            22582..22890
                     /locus_tag="VHD02_000809"
     CDS             22582..22890
                     /locus_tag="VHD02_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497432.1"
                     /translation="MYVFIEPNDLKDYLITVYDERNRAIRKQTQVYCFYLMIVLFVAL
                     FFFFPCLTIRDLVSYKLALIFAYLYLIIRSLVAKKMEALGKITTNSQKPPFDTSQVLC
                     "
     gene            complement(22973..23743)
                     /locus_tag="VHD02_000810"
     CDS             complement(22973..23743)
                     /locus_tag="VHD02_000810"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEQ5497433.1"
                     /translation="MKIGIIGVGKMASAIIKGLKQTPHELIISGSSLERSKEIAEQLT
                     LPYAMSHQDLIDQVDLVILGIKPQLFETVLKPLHFKQPIISMAAGISLQRLATFVGQD
                     LPLLRIMPNMNAQILQSSTALTGNALVSQELQARVRDLTDSFGSTFDISEKDFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKNGMPKAKALEIVTQTVLASASNLKTSSQSPHDFIDA
                     ICSPGGTTIAGLMELERLGLTTTVSSAIDKTIDKAKSL"
     gene            complement(23791..24858)
                     /gene="pepA"
                     /locus_tag="VHD02_000811"
     CDS             complement(23791..24858)
                     /gene="pepA"
                     /locus_tag="VHD02_000811"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEQ5497434.1"
                     /translation="MTDLFSKIKEVTELDGIAGYEHSVRDYLRTKITPLVDRVETDGL
                     GGIFGIRDSKAEKAPRILVAAHMDEVGFMVSDIKVDGTLRVVGIGGWNPLVVSSQRFT
                     LYTRTGQVIPLISGSVPPHFLRGANGSASLPHIEDIVFDGGFTDKAEAESFGITPGDI
                     IVPQSETILTANQKNIISKAWDNRYGVLMITEMLEALKGQDLNNTLIAGANVQEEVGL
                     RGAHVSTTKFDPELFFAVDCSPAGDIYGNPGTIGDGTLLRFYDPGHVMLKDMRDFLLT
                     TAEDAGVNFQYYCGKGGTDAGAAHLQNGGVPSTTIGVCARYIHSHQTLYAMDDFVEAQ
                     AFLQAIIKKLDRSTVDLIKRY"
     gene            25314..25604
                     /locus_tag="VHD02_000812"
     CDS             25314..25604
                     /locus_tag="VHD02_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEQ5497435.1"
                     /translation="MRDMSKKKIGMISGIFGFSLAIGLGIVIKDYCQDRKRRQMTRDL
                     RTFFSPLGQIEVLYINPCQVKQDYISGGVVMSNGKQYQFTYHSRQISFEEDK"
     gene            25604..25921
                     /locus_tag="VHD02_000813"
     CDS             25604..25921
                     /locus_tag="VHD02_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEQ5497436.1"
                     /translation="MIRPTSYESLAALVEKEDKLVLFFTADWCPDCQFIYPIMPEIEA
                     ELTDMTFVCVNRDQFIEVAQKWDIFGIPSFVVIEKGQEVGRLVNKMRKTKTEIMHFLA
                     AYQ"
     gene            25939..26565
                     /locus_tag="VHD02_000814"
     CDS             25939..26565
                     /locus_tag="VHD02_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEQ5497437.1"
                     /translation="MIFAYNKEQVGDVLMVILQDTKDIKRQVERKGKVARVFAEESGK
                     TLAWNIFEASSLITIEGNGQIFLTDEDLARLNAELAKEGFSERLEPTVGPVFVVGQIV
                     EMVAHLDSDHLNICQVAIGEDQTVQIVAGAPNAALGLKTIVALPGAIMPNGSLIFPGK
                     LRGEESYGMMCSPRELALPNAPQKRGIIELDESAVVGEAFDPAKHWKG"
     gene            26717..27112
                     /locus_tag="VHD02_000815"
     CDS             26717..27112
                     /locus_tag="VHD02_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEQ5497438.1"
                     /translation="MYNKVIAIGRLVAKPELVKTATDKHVARLSLAVNRRFKNASGER
                     EADFISVVVWGKLAETLVSYASKGSLISIDGELRTRKYDKDGQVHYVTEVLCQSFQLL
                     ESRAQRAMRENNVTNDLVDLVLEEDTLPF"
     gene            complement(27372..28013)
                     /locus_tag="VHD02_000816"
     CDS             complement(27372..28013)
                     /locus_tag="VHD02_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEQ5497439.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGEHLGTDVFYEAVDNNPVLDLYYQ
                     DPKKYAFLLQIYFLNKRFKSIKEAYQADNNILDRSIFEDELFLKLNYKNGNVTKTELD
                     IYQELLANMLEELEGMPKKRPDLLIYIDVSFDKMLERIEKRGRSFEQVDGNPSLEQYY
                     HQVHGEYPTWYEDYKVSPKMKIDGNSLDFVQNPQDLATVLKMIDTKLKELHLL"
     gene            complement(28033..29010)
                     /gene="dusB"
                     /locus_tag="VHD02_000817"
     CDS             complement(28033..29010)
                     /gene="dusB"
                     /locus_tag="VHD02_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEQ5497440.1"
                     /translation="MIKLNSSFRIGDVEIPHRTVLAPMAGVTNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQTNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPDKIYHIVKEVTSVLDIPLTVKMRTGWADSSLAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLARVSKAITKIPFIGNGDVRSVQDAK
                     FMIEEIGVDAVMIGRAAMNNPYLFTQINHFFETGQELPDLPFAKKLDIAKDHLKRLIN
                     LKGETIAVREFRGLAPHYLRGTAGAAKVRGAVSRAETLAEVEAIFETVR"
     gene            complement(28997..29869)
                     /gene="hslO"
                     /locus_tag="VHD02_000818"
     CDS             complement(28997..29869)
                     /gene="hslO"
                     /locus_tag="VHD02_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEQ5497441.1"
                     /translation="MDKIIKSIAQSGSFRAYVLDSTETVALAQEKHNTLSSSTVALGR
                     TLIANQILAANQKGDSRITVKVIGDSSFGHIISVADTKGHVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVTIIDYGTGNPYTSTTPLITGEIGEDFAYYLTESEQTPSAIGLNV
                     LLDENDKVKVAGGFMVQVLPGASEEEVARYEKRLQEMPAISHLLASKNHVDALLEAIY
                     GDEPYKRLSEEPLSFQCDCSRERFEAALMTLPKADLQAMIDEDKGAEIVCQFCGTKYQ
                     FNESDLEAIINDKA"
     gene            complement(30016..31510)
                     /gene="rofA"
                     /locus_tag="VHD02_000819"
                     /pseudo
     CDS             complement(30016..31510)
                     /gene="rofA"
                     /locus_tag="VHD02_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047149466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RofA"
BASE COUNT         9463 a         5708 c         6876 g         9590 t
ORIGIN      
        1 aaaatttaat attttacgga aagatagcga agaggctaaa cgcggcggac tgtaaatccg
       61 ctccttcggg ttcgggggtt cgaatccctc tctttccatc ctaaacgggc atagtttaaa
      121 ggtagaacta aggtctccaa aaccttcagt gtgggttcga ttcctactgc ccgtgttaga
      181 aatatggcgg gtgtggtgaa gtggttaaca catcagattg tggctctgac attcgtgggt
      241 tcgattccca tcactcgcct attttattgg ggtatagcca agcggtaagg caagggactt
      301 tgactccctc atgcgttggt tcgaatccag ctaccccagt ttaaatttta tatttaaagc
      361 cggcgtggcg gaattggcag acgcgctgga ctcaaaatcc agtgtccgca aggacgtgcc
      421 ggttcgaccc cggccgccgg tatacttgaa aaacaaggtt tacaaacctt gttttttgtt
      481 ttgatcttaa gctgtagcta cgtagtacaa tctcttatat tactgataag gtctatttaa
      541 tgcgcaagat ttgtggtatg atgaatatga ttgagtattg atgctttatg ggattaccgg
      601 taaggttttg aaaaaggttt gtcactacta ggtgcttttt tagctataga aagacagtta
      661 tggattgatg tattgtaact aatgttggag gttaatggtg gttgagtttt gagatattta
      721 cactgataat cgggtggaaa ctggttgggt atcgggtaat ggagtgtggt tcttcgttta
      781 agcaaggaga ttatcgtttg gtggttcata tttgcttgtt aaatgttagg gatgagatgc
      841 tgagtcagca aggccatgaa gataaagctg actggccaga gccttgagat gtgacgtttg
      901 gtggaagtcc tttttggtga gatggcacag catatagtaa tattagatcc tgaggaagaa
      961 ttgggacttt cattaggctt gactggggta tgacctcact ttacaattca tttcgatgat
     1021 gggtttgatg atacgttttt agttcaggac tttgtagagt tggagaattt ggttctttat
     1081 gaggaagaag tgcaggtaat tcgttgagac agtcgtgaag atattttagg tatgattgat
     1141 gaaggttcgt tcattcctta tctgaagtat aaaataaatt tgtgctttga gatggttggc
     1201 caatataaag tccatcaatc ttagaagtaa taatttttga attttttctg aggtttgtgt
     1261 atatttgata gtgtactgat gtaagaagta aggagtacta tgatggcaat caatgagaga
     1321 gcttctgtaa aaatcaagtt aggccttgat actaagaata agcgatgaga tggttaccac
     1381 gatttatcga tggtcatgat gagtattgat ctgtgtgact atgtgacggt tgaccatata
     1441 gatgacaata agattgtctt tgcctcaaac tgtccgaaaa tacctattaa ctaagataat
     1501 gatgtttata agatagttca gctaatgaaa catcgatttc aagttaaaag aggagtttcc
     1561 gtttacttgg aaaagcggat tcccatgtgt gctggtatgg gaggaggttc tagcgatgct
     1621 gttactatca gagcattaaa tcagttatgg ctccttactc ttagccgaaa agatatgatg
     1681 gatattggta taccgattgg tagtgatgtg tcttattgct tgctttcagg ttgtgcccaa
     1741 gtcactggta aaggtgaagt ggtttgtcgc attttggggc tgttatcgtc ttgggtggtt
     1801 ttggttaaac cagattttgg tatctcgact tgaacatttt ttctagatat taattgtaaa
     1861 atcatttcaa gagtgtctac tactcatttg gttgcggcga ttgaagcggg taactataat
     1921 gatgggatat tgactgaaat gaacaatttg ctggaagata ttttcattgc caagcgaccc
     1981 tttattcaaa aaataaaaga gaaaacactg caagctggtg ctgctactac tttgatgaca
     2041 ggaagtggac cgactgtttt tgctttgtgc cagaccgaaa aacaaactat aatagtttaa
     2101 aaggactttg caaggaagtt tttaaggtga gaatgttgta atattttgtt gggattgctt
     2161 ctttggaagc ttcatgttat aattaactgg ttaatgaaag aggagattta tggggacttt
     2221 agaaaaaaaa cttgataact tagtaaatac tattttatta aaagcagaaa atcagcatga
     2281 gttattattt ggagcttgtc aaagtgacgt taagcttact aatacgcaag aacatatttt
     2341 aatgttacta tctcagcaac gtctcactaa tacagatttg gctaaggcat taaatattag
     2401 tcaggcggca gtaactaagg ctatcaagag tttggtcaaa caagacatgc tagcaggaac
     2461 taaggatacg gttgatgcta gggtgactta ttttgaatta accgagttag ctaagcctat
     2521 tgcgtcagaa catacccatc atcatgatga aaccttaaat gtttacaacc gtttattaca
     2581 aaaattctcc gcgaaagaat tagagattgt agataagttt gtaacagttt ttgctgagga
     2641 attagaaggg taatccatga gatacatatc agtgaaaaat ctctcctttc aatatgaaag
     2701 tgagccagtt ttagaaggga tcacttatca tttagatagt ggagaatttg tcaccatgac
     2761 cggtgaaaat ggcgctgcaa agtcaacctt aataaaagca accttaggaa ttttacaacc
     2821 aaaggctgga cgagttacta ttgctaaaaa aaataaagac ggtaaacaat taaggattgc
     2881 ttacttgccg cagcaagtag ctagctttaa cgctggtttt ccatccaccg tttacgagtt
     2941 tgtcaaatca ggtcgctacc cacgtagtgg ttggtttaga catttgaaca aacacgatga
     3001 agagcatgtg caagcaagct tagaagcagt cggcatgtgg gaaaaccgtc ataagagaat
     3061 tggtagttta tcaggtggtc aaaaacaacg tgtggttatt gcccgtatgt ttgcttctga
     3121 ccctgatatt tttgtgctag acgagccaac aacgggaatg gatagcggta ctactgatac
     3181 cttttatgaa ctgatgcacc acagtgcaca tcaacacggg aaatccgttc tgatgattac
     3241 ccatgaccca gaagaagtga aggcttatgc tgatcggaac attcatttag tcagaaacca
     3301 aaaacttcct tggcgttgtt tcaacattca tgaagttgaa acagatgacg aaaaaggagg
     3361 tcatggtcat gcttgatatt ttatcctatg atttcatgca acgggcggta atggcggtag
     3421 ttgccattag tatttttgct ccgattttag gtattttcct tattttacgt cgtcaaagtt
     3481 tgatgagcga tacccttagt catgtttctt tggctggggt agcgcttggg gtagtccttg
     3541 gtatttcacc aaccatcact actattattg ttgtggtttt agctgctatt ttgttagaat
     3601 acctgcgtgt agtttacaaa cactacatgg agatttcaac ggcgattttg atgtcacttg
     3661 gcttggccct atctctgatt attatgagta agtcgcatag ttcatcaagc atgagtttag
     3721 aacaatacct ttttggatcg atcatcacga ttagtatgga acaagttgtc gccttgtttg
     3781 ctattgctgc gattatttta atcttgaccg ttctcttcat tagaccgatg tacattctga
     3841 cctttgatga agatactgct tttgtagatg gtttgcccgt tcgcttgatg tctgttctat
     3901 tcaatatcgt cactggggtt gctattgctt tgaccattcc agcagcagga gcacttttgg
     3961 tttctaccat tatggtcttg ccagcaagta tcgcaatgag attgggtaaa aactttaaaa
     4021 cagttatctt actgggaatt gtcatcggtt ttagcggtat gttatctggt attttcttat
     4081 cttatttctt tgaaacgcca gctagtgcca ctattaccat gattttcatt agtattttcc
     4141 tcttagttag tctaggtgga atgcttaaaa aacggttatt ttaataagaa aaatcctcac
     4201 atgtttaaca tgtgaggatt tatttataaa tgctttaaaa agggggtaag gtcccaattt
     4261 tcttgattaa tggtgatgct ctcccaaaag ctatcattag ttaaacgagg aatcaaatga
     4321 aggtgaaaat gagtcccctc atccatcaac tccttgtcat tgctgacgct agtgacgcct
     4381 gaaatgggaa ggtgctgctc tagcttagca actagagcgg cttgtaagtc gatgagatca
     4441 tagcgcactt cctttggtag ttgtgtcaga ctagtgtaat ggtctttgga aatcagtaaa
     4501 aggtgcccct tttggatagg atcaatatca aaaacaacct tgaaatattg actttgagtc
     4561 agtagctgat ttgctttgag ctgatggcaa aagatacatt ctgtcatagg tacatctcct
     4621 tatgaaaaaa cgagccagag ctcgtttaat cggttatcta gtaagtgaga acagcgtatt
     4681 ttttcttacc gcggcgaata acggttaatt gatcatcaat tttatcatca ttacttaatt
     4741 ggtaatctaa atcttgaacg cggtctccgt taatgtagat tgctccattt tgcacgtctt
     4801 cacgcgcttg gcgttttgat ggggaaatac ccgcggtgac aagcatgtca acaaggttaa
     4861 ggctgtcttc tgattgaacg tgataatttg gtacattgct caatccttgt tttaattcat
     4921 tagcagaaag atttttgatg tttccagcaa ataactgctc ggtaatgtta agagcttgtt
     4981 tataagcttc ttcgccatga accaaggtaa ccacttcgcg tgccaaggtt ttttgagcga
     5041 gacgctcgtg acgagcggca ttaaattgag tttcaatttc tgcaatctca tctagagata
     5101 agaaggtgaa gattttcaag aaacgcactg catcatcatc catgacattt aaccagaatt
     5161 ggtacatctc gtaaggagac gtcttatcgg catcaagcca gacagcatta ccttctgatt
     5221 taccgaattt tttcccggtt gagtcagtga tgagtggtac agtcatgacg tgcccagttt
     5281 tatcagcctt tttgcggagc aattcagtac cagctgtcat attaccccac tggtcagagc
     5341 cgccaatttg taaggttaca ttatgcttgt cattgagttc gtagaagtcg tagccctgca
     5401 tgatttggta agcaaactca gtgtaagaaa tgcctgtttc aatgcgtttt ttaacagagt
     5461 ctttactcat catgtagtta acggtgaagt atttaccgac atcacggagg aagtcaataa
     5521 agctgatttg cgagaaccag tcgtagttgt tgacaagctc tgctttatta tcaccatttt
     5581 caaaatcaag gaaagtagac aactgccctt taatcttgtc actccactcc aaaactgttt
     5641 ctttggtttg aaggctgcgc tctgcatctt taaaggaagg atcaccaatt aaacctgtgg
     5701 caccacccac aagagcgtaa ggtttgtgcc ctgctagttg caagcgacga gatgttaaga
     5761 tagccactag atggcccaga tgaaggctgt cagcggttgg atcataaccg gtataatagg
     5821 atacttgccc ttctgttaat gctttgacaa gggcttgttc atcagtcgtt tgaaagacca
     5881 agccacgagc tttgagttct tcaaaaatat tcataggctt tctccatttt cattatagtt
     5941 tcgaatcatt atatcaaaaa acgctactgc tatgcaagct ttatcaaagt ttggtataat
     6001 gataaatgag gtattttatg gtgaaatgga acacgaaaca aaagcgtata agtcatcaaa
     6061 gattaggtct cttggatttg gggccggtgc tattacgtac gttgagacta ctgtctaact
     6121 ttttttatat tgttatcttc ctttttggaa tgatgggatt tggtatggca tttgggtatt
     6181 tggctagtca gattgaatct gttaaggtac caagtaaaga aagtttagtc aaacaagttg
     6241 aatcattaac gatgatttcg caaatgaact attctgataa tagtttaatt tctactttag
     6301 atacggattt acttcgaaca ccagtagcta atgatgcgat ttcagagaat atcaaaaaag
     6361 ctattgtatc aacagaagac gaacattttc aagaacataa aggtgtcgtg ccaaaagctg
     6421 tttttcgggc aacattggct tctgtattgg gatttggaga agctagtgga ggttcgacct
     6481 taacacagca attagtcaag caacaagttt tgggagacga tcccacattt aagcgcaagt
     6541 ctaaggagat cgtttacgct cttgccttag agcgttatat gtccaaagac aatatcttat
     6601 gtgattatct taatgtttca ccttttggcc gtaacaacaa gggtcaaaat attgctggtg
     6661 ttgaagaagc tgcgcgtggc atttttggcg tttctgccaa agatttaacg gtgccacagg
     6721 cagcattttt ggcgggtctt ccgcagagtc ctattgttta ctctccttat ttgtcaacgg
     6781 gacaactgaa gtcagaaaag gacatggctt atggcatcaa gcgtcagcaa aatgttctct
     6841 ataacatgta ccgtacaggt gttctgtcta aaaaagaata cgaggactat aaggcttatc
     6901 cgattcaaaa ggattttatt caaccgggaa gtgcaatagt aaataatcac gattaccttt
     6961 attacacggt gttagcggat gctaaaaaag ccatgtatag ctatttgatt aagcgagata
     7021 aggtgtctag tcgtgacttg aaaaatgacg agactaaggc tgcttatgaa gagagagcct
     7081 taacagaatt gcaacagggt ggctatacca tcaccacaac cattaataag cctatttaca
     7141 atgcgatgca gacagcggca gctcagtttg gtggcctgtt agatgatggc actggtacag
     7201 ttcaaatggg aaatgtcttg acagacaatg cgactggtgc tgtgttaggt tttgttggtg
     7261 gtagagatta tgctctgaat caaaataatc atgctttcaa tacagttaga tcgccaggtt
     7321 ctagcattaa accgataatc gcttatggtc ctgctattga tcaaggttta atggggagtg
     7381 ctagcgtttt gtctaattac ccaacaactt actcgagtgg ccaaaaaatc atgcatgctg
     7441 atagtgaagg aacagccatg atgccacttc aagaggccct aaatacttct tggaacatcc
     7501 cagctttttg gacacagaaa ttactgcgtg aaaaaggggt cgatgtcgaa aattatatga
     7561 caaaaatggg ttataagatt gcagactact cgattgaaag tttacctcta gggggcggta
     7621 ttgaagtctc ggttgctcaa caaaccaatg cttaccaaat gctttcaaac aatggcttat
     7681 atcaaaagca atatattgta gataagatta ctgctagcga tggtacagtc gtttacaaac
     7741 atgaaaataa gccaattcgt attttttctg cagcaacagc tacgatttta caagaattgt
     7801 tgagaggtcc gattacttca ggcgctacga ctactttcaa gaaccgtttg gcggctatta
     7861 atccgtggct tgctaatgct gattggattg gtaagaccgg aacaactgag aattatacgg
     7921 atgtttggct agtcctgtct actccaaaag ttaccttagg cggttgggca ggacatgatg
     7981 acaatacctc attagcgcca ttaacaggat ataacaataa ttctaattac cttgcctatt
     8041 tagctaatgc cattaatcag gccgatccca atgttattgg agtagggcaa cgtttcaact
     8101 tagatccagg agtcattaag gcgaatgtct tgaagtcaac aggtttacaa ccaggaactg
     8161 ttaatgtcaa tggacatact ttttctgttg gtggagaaat gaccaccagt ctatggtccc
     8221 aaaaaggacc gggggctatg acttaccgat ttgctattgg tggcacggat gccgattatc
     8281 aaaaagcttg ggggaacttt gggttcagaa aaaattaggt tgccatttat cgataaaaag
     8341 ggtataatag caataactat ttgtcgtcca atctagctga aatattgtcc agttaggaag
     8401 aacagcagtt aaatcacact gataaagtca gatttagctg ctctttttgt gtctattttt
     8461 agaaaagata gggtttgtaa cctatattta aatattctaa aaattaacat ttagggcaaa
     8521 tgataactta gttgcgattt gctgaaacag caaagcttaa atagaagaag gagctaaaaa
     8581 cttggcagga catgaagttc gatacggaaa acaccgtaca cgtcgtagct tttcaagaat
     8641 caaagaagtt cttgatttac caaatttgat tgaaattcaa actgactcat tccaagattt
     8701 cctcgattca ggtttgaaag aagtatttga agatgtactt cctatttcaa actttacgga
     8761 tactatggaa cttgaatttg ttggttacga atttaaagaa cctaaatata cccttgaaga
     8821 agctcgtatc cacgatgcaa gttattctgc accaatcttt gttaccttcc gtttggtcaa
     8881 taaagaaact ggtgagatta aaactcaaga agtcttcttt ggtgatttcc caatcatgac
     8941 ggaaatgggt acctttatta tcaatggtgg tgaacgtatc atcgtttctc agttggtgcg
     9001 ttctccaggt gtttatttca acgataaagt tgataaaaac ggtaaagttg gttacggatc
     9061 aacagtgatt cctaaccgtg gggcttggtt ggaattagag actgactcaa aagacattgc
     9121 ctacactcgt attgaccgta cccgtaagat tccattcaca accttggttc gtgcccttgg
     9181 tttctcaggt gatgatgaaa ttgttgatat ctttggtgaa agcgaccttg ttcgtaatac
     9241 cattgaaaaa gacattcaca aaaatccaag tgattctcgt acagacgaag cccttaaaga
     9301 aatttacgag cgtcttcgtc caggtgagcc aaaaactgca gatagctctc gtagtctttt
     9361 gattgcgcgt ttctttgatg cacgtcgtta cgatttggct gcggttggtc gttacaaagt
     9421 gaacaaaaaa cttaatatca agactcgtct tttgaaccaa atcattgctg aaaaccttgt
     9481 ggatgctgaa actggcgaaa ttttggtaga agctggaact gagatgactc gtagcgtcat
     9541 cgaatccatc gaagaacacc ttgacggtga tttgaacaag tttgtttaca caccaaatga
     9601 ttacgctgtg gttactgaac ctgttgttct tcaaaagttc aaggttgtgt caccaattga
     9661 tcctgatcgt gtagtaacta ttgttggtaa tgccaatcca gatgacaagg ttcgtgcatt
     9721 gacacctgct gatattttgg cagaaatgtc ttacttcttg aaccttgcag aaggtcttgg
     9781 aaaagttgac gatattgacc acttaggtaa ccgtcgtatc cgtgccgttg gtgaattgct
     9841 tgccaaccaa ttccgcatcg gtcttgctcg tatggagcgt aacgttcgtg agcgtatgtc
     9901 tgttcaagac aacgatgtgt taacaccaca acaaatcatc aatatccgtc ctgtcacagc
     9961 agctgtcaaa gaattcttcg gttcgtctca gttgtcacaa ttcatggacc aacacaaccc
    10021 attgtcagag ttgtctcaca aacgtcgttt atctgcctta ggacctggtg gtttgacacg
    10081 tgaccgtgct ggttatgagg ttcgtgacgt gcattacacg cactatggcc gtatgtgtcc
    10141 gattgaaaca cctgaaggac caaacattgg tttgattaac aacttgtctt catttggaca
    10201 tcttaataaa tatggtttca tccaaacacc ttaccgtaag gttgaccgtg cgactggtag
    10261 ggtaactaac gagattgttt ggttgactgc cgacgaagaa gacgaataca cagttgcaca
    10321 ggccaattcg aaactaaacg aagatggcac ttttgctgaa gaaatcgtta tgggtcgtca
    10381 ccaaggtaat aaccaagaat tttctgcaag tgttgttgat ttcgttgacg tttcccctaa
    10441 acaggtagtt gctgttgcga cggcatgtat tcctttcttg gaaaacgatg actccaaccg
    10501 tgccctcatg ggtgccaaca tgcaacgtca ggctgtgcca ttgattgatc caaaagcacc
    10561 atatgttggt actggtatgg aatatcaagc tgcccatgac tcaggcgctg cggtgattgc
    10621 tcagcacaat ggtaaagttg tcttttctga tgctgaaaaa gtggaaatcc gtcgtcaaga
    10681 tggctcactt gatgtttacc acattaccaa attccgtcgt tcaaactcag gaacagccta
    10741 taaccaacgc acccttgtta aagtaggaga cattgttgaa aaaggtgatt tcatcgctga
    10801 tggaccttct atggaaaatg gtgaaatggc tcttggacaa aacccagtcg ttgcttacat
    10861 gacttgggaa ggttataact ttgaggatgc cgttatcatg agtgagcgcc ttgtgaaaga
    10921 agatgtctac acatctgttc acttggaaga attcgaatct gaaacgcgtg atacaaaact
    10981 tggccctgaa gaaatcactc gggaaatccc aaatgttggt gaagaagccc tcaaagacct
    11041 tgacgaaatg ggcattatcc gtatcggtgc tgaggtgaaa gaaggcgaca tcttagtagg
    11101 taaagtcaca cctaaaggtg aaaaagacct ttctgctgaa gaacgtttac ttcacgccat
    11161 ctttggggat aaatcgcgtg aagtgcgtga tacctcactt cgtgtccctc atggtggtga
    11221 tggtattgtt cgtgatgtga aaatctttac acgcgctaac ggcgatgaat tgcaatcagg
    11281 tgttaatatg cttgtgcgtg tttacatcgc tcaaaaacgt aaaatcaagg tcggagataa
    11341 aatggccggt cgtcacggaa acaagggtgt cgtttcacgt attgtaccag ttgaagacat
    11401 gccatacctt ccagacggaa caccagttga catcatgttg aaccctctcg gggtgccatc
    11461 acggatgaat attggtcagg ttatggaact tcaccttggt atggctgctc gtaatcttgg
    11521 cattcacatt gcaacacctg tctttgacgg ggcttcatca gaagaccttt gggacactgt
    11581 tcgtgaagct ggcatggata gcgatgctaa gacggtcctt tatgatggtc gcaccggtga
    11641 accatttgac aaccgtgtat ccgttggtgt catgtatatg atcaaacttc accacatggt
    11701 tgatgataaa cttcatgccc gttctgtagg accatactca cttgttaccc aacagccact
    11761 tggtggtaaa gctcaatttg gtggacaacg ttttggtgag atggaggttt gggcccttga
    11821 agcttatggt gcatcaaatg ttcttcaaga aatcttgacc tacaagtcag atgacgtcac
    11881 aggacgtttg aaagcctatg aagcgattac taaaggtaaa ccaattccaa aaccaggtgt
    11941 accagaatcc ttccgtgttc ttgtaaaaga attgcaatcg cttggtcttg atatgcgtgt
    12001 gcttgacgag gatgataatg aagtggaact tcgtgatctt gatgaaggtg aagacgatga
    12061 tattatgcat gttgacgatc tcgagaaggc acgtgaaaaa caagctcaag aaacccaaga
    12121 agtttctgaa acaactgacg aaaaataagc aatcaattct tattaaataa ttatttactg
    12181 gtctggggca aaggccccag gaactggtaa agtcatcaaa ggcagaaagg taaaactagt
    12241 ggttgacgta aatcgtttta aaagtatgca aatcacatta gcctcaccaa gtaaggtccg
    12301 ttcatggtct tatggtgaag ttaaaaaacc tgaaacaatc aactaccgta cattaaaacc
    12361 agaacgtgaa ggactctttg acgaagtcat ctttggtcca acaaaagact gggaatgtgc
    12421 gtgtggtaag tacaaacgta tccgttataa agggatcgtt tgtgaccgct gtggggttga
    12481 ggtaactcgt gctaaagtac gtcgtgaacg tatgggtcac attgagttaa aagctcctgt
    12541 atcacatatc tggtatttca aagggattcc atctcgtatg ggattgactc ttgatatgag
    12601 tcctcgtgcc cttgaagaag tgatctattt tgcggcttat gtggtcattg atccaaaaga
    12661 tacaccgctt gaaccaaaat cattattaac agaacgtgaa taccgtgaaa aactacaaga
    12721 gtatggtcat gggtcatttg tcgctaagat gggtgctgaa gccattcaag atctcttgaa
    12781 acgtgtggac ttggcagctg aaatcgctga attaaaagaa gagttgaaat ctgcttctgg
    12841 tcaaaaacgg attaaagcag ttcgtcgttt agacgtcctt gatgccttta acaaatctgg
    12901 aaataaacca gaatggatgg ttcttaacat cctgccggtt attccaccag atcttcgtcc
    12961 gatggttcaa ttggatggtg gtcgttttgc ggcatcagac ttgaatgact tgtaccgtcg
    13021 tgtgattaac cgtaacaacc gtttggcacg tttgttagaa cttaatgccc ctggcatcat
    13081 tgttcaaaat gaaaaacgaa tgcttcaaga agccgttgat gctttgattg ataatggtcg
    13141 tcgtggtcgt ccaatcactg gaccaggtag tcgtccattg aaatcattga gccacatgct
    13201 taaaggtaaa caagggcgtt tccgtcaaaa cttgcttggt aaacgtgtag acttctctgg
    13261 acgttccgtt atcgctgttg gtccgaccct taaaatgtat caatgtggtg tcccacgtga
    13321 aatggctatc gagcttttta aaccatttgt aatgcgcgaa attgttgcca aagaatatgc
    13381 tggtaacgtt aaagccgcta aacgtatggt tgaacgtggc gacgaacgca tctgggatat
    13441 cttagaagaa gttatcaaag aacacccagt cctacttaac cgcgcaccga ctcttcacag
    13501 acttggtatt caggcttttg aaccggttct tattgacggt aaggcacttc gtcttcaccc
    13561 acttgtgtgt gaggcctaca atgccgactt cgatggagac caaatggcca tccacgtgcc
    13621 actttcagaa gaagcacaag ctgaagctcg tcttttgatg cttgctgcag agcacatctt
    13681 gaaccctaaa gatggtaaac cagttgttac cccatctcag gatatggttc ttggtaacta
    13741 ctatcttacg atggaagatg ctggtcgcga aggcgaaggt atgattttca aggataaaga
    13801 cgaagctgtg atggcatatc gtaatggcta tgctcatctt catagtcgtg tgggtattgc
    13861 tgttgacagc atgccaaaca aaccttggaa agacagtcaa agacataaaa tcatggtgac
    13921 aactgttggt aagattttgt ttaacgacat catgccagag gaccttcctt acctccaaga
    13981 gccaaacaat gccaacttga cagaaggaac acctgataaa tacttccttg aacctggtca
    14041 agacatccaa gaagtgattg acggcttaga catcaatgtg ccatttaaga agaaaaacct
    14101 cggtaacatc attgcggaaa ccttcaaacg tttccgtaca acagaaacat cagccttcct
    14161 tgaccgcttg aaagaccttg gttactacca ttcaaccctt gctggtttga cagtgggtat
    14221 cgctgacatt cctgttattg ataataaagc tgaaatcatt gatgctgctc accatcgtgt
    14281 tgaagaaatt aacaaagcct tccgtcgtgg tttgatgaca gatgatgacc gttatgttgc
    14341 cgttacaaca acatggcgtg aagctaaaga agcccttgaa aaacgtctga ttgaaacaca
    14401 agatcctaag aacccaatcg ttatgatgat ggactcaggt gcccgtggta acatctctaa
    14461 cttctcacag cttgccggta tgcgtggttt gatggctgct cctaacggac gcatcatgga
    14521 acttcctatc ttgtcaaact tccgtgaagg tttgagcgtt ttggaaatgt tcttctcaac
    14581 ccacggtgca cgtaaaggga tgaccgatac ggcccttaaa acagccgact caggttatct
    14641 tactcgtcgt ttggttgacg ttgcccaaga tgttatcatt cgtgaggacg attgtggcac
    14701 tgatcgtggt cttcttatcc gtgctattac agatggtaaa gaagttaccg aaacgcttga
    14761 agagcgtctt caaggtcgtt acacacgtaa atcagtcaaa caccctgaaa ctggtgaagt
    14821 cttgattggt gctgaccaat taatcactga agacatggct cgtaagattg ttgatgcagg
    14881 tgttgaagaa gtgaccattc gttctgtctt tacctgtgcg actcgtcatg gtgtctgccg
    14941 tcactgttat ggtatcaact tggcaactgg tgatgctgtt gaagtgggtg aagcagttgg
    15001 tactattgct gcccaatcta tcggtgagcc tggtactcag cttaccatgc gtaccttcca
    15061 cacgggtggt gtagcctcaa ataccgatat cacccagggt cttcctcgta ttcaagagat
    15121 ctttgaagca cgtaatccta aaggggaagc ggtcattact gaagtgaaag ggaatgtcgt
    15181 cgagattgaa gaagatgcgt caactcgtac caagaaagtc tacgttcaag gtaaaactgg
    15241 catgggcgaa tatgtcgtac catttacagc acgtatgaaa gttgaagttg gcgacgaagt
    15301 taatcgcgga gctgccctta cagaagggtc aattcaaccg aaacgtctcc ttgaagtgcg
    15361 tgataccttg tcagttgaaa cgtaccttct tgcagaagta caaaaagttt accgtagcca
    15421 aggggtagaa atcggagaca aacacgttga ggtaatggtt cgccaaatgc ttcgtaaagt
    15481 tcgtgtcatg gatccaggtg atacagacct ccttccaggt acacttatgg atatttctga
    15541 tttcacagat gctaacaaag atattgttat ctctggtggt atccctgcga catctcgtcc
    15601 tgttcttatg ggtattacta aggcttccct tgaaaccaat tccttcttat cagctgcatc
    15661 cttccaagaa acaactcgtg ttcttacaga tgctgctatc cgtggtaaga aagaccactt
    15721 acttggtctt aaagaaaatg ttatcattgg taaaatcatc ccagcaggta ctggtatggc
    15781 gcgctaccgt aacattgaac cacaagcgat gaatgagatt gaagtgattg atcatacaga
    15841 agtctcagca gaagcagaat aatctcatga aagagtcctt aggggctctt tcttgtctga
    15901 gtttgttggc agtaaaaagc attgacaatc agtcttagtc ccaatgagca gatgtttttc
    15961 aaaatactta tgatttagct ataataatgc ataagaggaa tgggtaatgt gttatgtatc
    16021 aagtaattaa aatgtatggt gattgggagc cttggtggtt catcgatgga tggcaagacg
    16081 atattataga tgagcaacaa tttagtgact ggcaagaagc gcttgattat tttaatcaag
    16141 aatggcagcg catgaaagct atttttccta gttatcatag ccaaaagaat ttgttagcta
    16201 ctttttggga aaaagaagat aaaagatggt gcgaggactg tgacgaagat ttgcagcagt
    16261 ttcattctct tttactcctt aaaaataaag atattgtacc aagcaataat tatattcctg
    16321 aatttgaaca acgaaatgat tcaccacagg tagcttatct ttgcaagtta aacctttaag
    16381 tgtttgttaa aaacaaaata agttaaaaga aaatatcatt tcttatgata ttttcttttt
    16441 ttgtttacga ataaatgagt aaggaggaat tatggtacaa gcattagcaa aagctattct
    16501 agcaaaagct gaacaggttc aggcacaaga tatttatatt ttgccaagag cagatcaata
    16561 tgatcttttt ttacgaatag gagatgaaag gagattagtt gatgtttatc agagcgatcg
    16621 gatggctcct cttattagtc actttaaatt cgttgcagga atgatagttg gtgaaaagag
    16681 acgttgtcag gtgggttcat gtgattataa gcttagtaaa gataagcagt tatctttgcg
    16741 cttatctagc gtgggtgatt atcgcgggca agaaagctta gtgattcgtc tgcttcatca
    16801 tcaaaataaa agtgtacatt attggtttga tggattgaca aaagtagcca atcaagttgg
    16861 cggtagaggg ttgtatttat ttgcaggacc agttgggtct gggaagacaa ccttgatgta
    16921 ccagctgatt tcgagttatc atcaagaagc acaggttatt agtatagaag atcctgtaga
    16981 aattaaaaat caccaaattt tacaattaca agtgaatgat gatattggta tgacttatga
    17041 caatttgatc aaactgtctt tacgccatcg accagatatt ttagttattg gtgagattcg
    17101 agatagtcaa acagcaagag ccgttattag ggctagtcta acaggtgcca tggttttttc
    17161 aacggttcac gctaaaagta tctcgggtgt ttatgcaaga ttgttagaac ttggtataac
    17221 gaaagcagaa ctgtctaatt gcttagcatt aattgcttac caaaggttac ttaatggagg
    17281 agcattgatt gactctactc aaaacgaatt tgaatattat tcctcatcga actggaatca
    17341 acaaattgat cagcttcttg aggcaggaca tctcaatccc aagcaagcta agcttgaaaa
    17401 aattatctag taaacatcag cataaattca ttcaattact agctaatctt ttatctacag
    17461 gatttagctt cgcagaggtt attgcttttt taaaaagaag ccagttgctt cagctagatt
    17521 atgttcttaa aatggaagag tctttattaa aaggacaagg cttagcagat atgttgtcgg
    17581 gtttaggttt ttcagatgct attcttactc aaattagttt agctgataga cacggtaata
    17641 ttgaaacaac attagtagcg attcaacatt acttgaacca aatggcaagg atcagacgaa
    17701 aaactgttga agttatcacc tatcctctta ttcttttact ttttcttttt gtgatgatgc
    17761 tagggttgag acgctattta gtccctcaat tagaaactca aaatcagata acttactttc
    17821 ttaaccattt tcctgccttc tttataggtt tttgctcagg tcttattttg ttgttcggga
    17881 tggtatggct acgatggcga tctcaaagcc gcttgaaact ctatagtcgg ctaagtcgtt
    17941 atcctttttt aggtaaactg ttaaaacaat acttaactag ttattatgct agagaatgga
    18001 gtacgttaat aggtcaaggt cttgatttga tgactatttt agacattatg gccattgaga
    18061 aatcctcact aatgaaagaa ttagctgagg acatcagaat gagtctgctt gaagggcaag
    18121 cctttcacat taaagtagct acctaccctt tttttaaaaa agaattaagt ttaatgattg
    18181 aatacggtga aatcaaatcg aaactggggg ttgagttgga aatctatgcc caggaaagct
    18241 gggaacaatt ttttagtcag ctttatcaag tgacacaact tattcaacca gctatttttt
    18301 tagtagttgc tgtgacaatc gtcatgattt atgcggcaat cctattacca atttaccaaa
    18361 atatgggagg tattttttaa gatgattaat caatggaaca acttacgaca caagaagtta
    18421 aaaggattta ctcttctaga aatgttattg gtgattcttg tcatcagtgt tttgatgcta
    18481 ttatttgtgc ctaatttaag caagcaaaaa gacagggtta cagaaacagg taatgccgct
    18541 gttgttaaat tagtggagaa tcaagcagaa ctatatgaat tatctcaagg ctcaaaacca
    18601 agtttgagcc agttaaaggc agatggtagt atcactgaga aacaagaaaa agcttatcaa
    18661 gactattatg acaaacataa aaatgaaaaa gcccgtctta gcaattaaag cttttacttt
    18721 gcttgaaact ttactaagtt tgtcagtgat gagtttcatt atattgggat tgtctgtacc
    18781 agtgacaaaa agctatcaaa aagtagaaga gcatctgttt tttagccatt ttgaacattt
    18841 gtatcgacat cagcaaaagt tagccatatt acagcaaaaa cagcgcgtct tggatatttc
    18901 ttcaacaaaa attgttacag aaggtaatag cctaacagtt cctaaatcaa taacagtgaa
    18961 tcatccttat cggttagtta ttgatcagat gggaggcaac cattccttag ctaaaattat
    19021 ttttgacatg actgatcgac gctttaaata ccaattttat ctagggagtg ggaattatca
    19081 aaaaacaagt caaagcttac atagcccttg aaagtatgat tgcaacaggc atcctcttta
    19141 gtattgtcat tctcgtctta agcagtttac agcagagtca ggctgctcta acgtactatc
    19201 gaaagcagca agaaaagctt aatctagcct tgatggcagt acaaactaga actaaagaga
    19261 tgacattgaa tggttgtcat attactattt tacgtacgga tagatacatt agtattcacg
    19321 atgacgaagg agaagtgatg aagattgagt aaacaattaa gtaacataaa agcttttacc
    19381 ctcctagagg cgttaatagc cttactcgtg atatcagggt ctttattggt ttatcaaggt
    19441 ttgacccaaa ccctccttaa acgtagccat tacctagccc gtcatgatca agacaattgg
    19501 ctcttatttt ctcatcaatt gcgagaggag ttaagtggag caagattttg caaagtagct
    19561 gataataaac tatacgttga aaaaggaaag aaagtactag cttttggcca atttaaaagt
    19621 catgattttc gaaaatcagc tagtaatgga aaaggatatc aacccatgtt atttggaata
    19681 tcacgtagtc atattcacat agagcagtca cagatttgca ttactttaaa gtggaaaagt
    19741 gggttagaaa ggacttttta ttatgccttt caagactaat ctcaaagcag gcgtcctttt
    19801 gtatgctatg tttatggcta gtattttttt gctggtttta caggtttacc tttctcaagt
    19861 gacagctcta cacaaggaat accaagctca aactgactat gttaaagccc gactgatagc
    19921 agaaatagtt tatcaagatc atagatataa agcttctaat cctgtctttt ttaaaggtgg
    19981 tcaagtcatt tgtcgagagc gaaaagagag atggatgtta attgttaagt tagatcagca
    20041 acgacagtat cagtttgaat accttaaacc caaacaatat agtcaataaa tagtattgtt
    20101 gtcataaatc cttaaaatga tccaatatat agtaattaaa ggtgacactt cttttcattt
    20161 caagtgtaat ttggtatgat aaggttatga cttttgaaaa aattgaagaa gcttatcagc
    20221 tgctcttaga gaactgtcaa ctaattgaaa atgatctaaa aacacatata tacgatgcca
    20281 ttgttgaaca aaactccttt tatttagggg ctgagggagc tagtcctcaa gttgctcaaa
    20341 atagtgataa actgaaagcc ttgtgcctga caaaagaaga atggcgtaag gcctaccagt
    20401 ttctttttat taaggcagct cagactgagc aactccaagc caaccatcag ttcacaccag
    20461 atgctattgg cttcattctg ctgtatcttt tggaacaatt gagtgataaa gatagcttag
    20521 aggtacttga gattggaagt ggaacaggga acctagccca aacccttctc aacaacacga
    20581 gcaagagcct tgattatgta gggattgaac ttgatgatct cttgattgat ctgtcagcca
    20641 gtattgctga gataatggat tcttcagctc attttattca agaagatgcg gtaaggcctc
    20701 aattactaaa agaaagtgac attgtcatca gtgacttacc agttggttat tatcctaacg
    20761 atgatattgc caaacggtac aaggtggcta gttcagataa gcatacctat gcccatcatt
    20821 tattaatgga acagtcttta aaatacttga aaaaagacgg ttttgcgatt tttctggcac
    20881 cagtcaattt attgacgagc cctcagagtc agttattgaa acagtggtta aaagattatg
    20941 ctcaggtggt ggccttgatt acgctaccag attctatttt tggtcatccc tcaaatgcca
    21001 agtccattat tgtcttacaa aaacaaacag accacccaat ggaaaccttt gtctatccaa
    21061 ttcgggattt gaagcttgca gagaatattc atgattttat ggaaaatttc aaaaaatgga
    21121 aactgagtaa tgtcaattaa ttgatttttt gttataataa aaaaacatta cagaaaacga
    21181 tttcaagaga ggttaaaaat gtcaaagaca attgcaatta atgcaggtag ttctagttta
    21241 aaatggcaac tttatcagat gccagaagaa gaagtgttag cacaaggaat tattgagcgt
    21301 atcggtctca aggattctat ttcaacagtt aagtacgatg gcaaaaaaga agagcaaatt
    21361 cttgatattc acgatcatac agaagctgtt aaaatcttat tgaacgattt gattcacttt
    21421 ggaatcattg cagcttatga tgaaattaca ggagttggac accgtgttgt tgctggtggt
    21481 gaacttttca aagaatccgt tgtggttaat gacaaagttc ttgaacagat tgaagaatta
    21541 tccgttttag ctccacttca taatcctgga gcggcggcag ggattcgtgc cttccgtgat
    21601 attttgccag atattaccag tgtttgtgtg tttgacacgt catttcacac cagcatggca
    21661 aaacatactt acctttaccc aattcctcaa aaatattata cggactacaa ggttcgtaaa
    21721 tatggagctc atgggacaag tcataaatat gtggcgcaag aagctgctaa aatgcttggt
    21781 cgtccattag aagaattaaa actaattact gctcatattg gaaatggtgt ctcaattaca
    21841 gctaattacc acggtaaatc agttgatacc tcgatgggct ttacaccact tgctggtcct
    21901 atgatgggaa cacgttctgg tgacattgac ccagctatca ttccttattt aattgagcaa
    21961 gacccagagt taaaagacgc tgctgacgtt gttaatatgt taaacaaaaa atcaggtctt
    22021 agtggggtgt caggcatttc tagtgatatg cgtgatatcg aggctggttt acaagaagat
    22081 aatccagatg ctgttttagc ttacaatatc tttattgatc gcattaaaaa atgcattggt
    22141 caatattttg ctgttttgaa tggagcagat gctttggtct ttacagctgg tatgggtgaa
    22201 aatgcaccat tgatgcgtca agatgttatt ggtggcttaa catggttcgg aatggacatt
    22261 gatcctgaga aaaatgtttt tggataccgt ggtgatattt caacacctga gtcaaaagtg
    22321 aaggttcttg ttatctcaac agacgaagaa ttgtgcatcg cgcgtgacgt tgagcgtttg
    22381 aaaaatacaa aataaaagag actaagtctc aatagattaa ccaagtaagg caacacttta
    22441 cttggttttt tatgaggttt atctgttagt tatggtgcct aggtattagt tctgattagt
    22501 ctccttgctt tttgtaatct tgtgctaggc gatttggatg tcagccttat ttagaagttt
    22561 ttaattgctt caacgctttt cttgtatgtg tttatagagc ccaatgatct aaaagactat
    22621 ttgataacgg tttatgatga gcgtaatcgt gcgattcgaa aacagacaca agtttactgc
    22681 ttttacctga tgattgttct atttgttgcc ttattctttt tcttcccttg cttaaccatt
    22741 cgagaccttg tttcttataa gctagctttg atttttgctt acctttactt gattatcaga
    22801 agtctggtgg ctaagaaaat ggaggcacta gggaaaataa caactaatag tcaaaaacct
    22861 ccgtttgata cgagtcaggt tctttgttag atgacttgtc taacggttga gtttcctgtc
    22921 tggtcaacgg aggtgtttgt attgttgtct gatttgtatt ggggcactta ttttacaagc
    22981 ttttagcctt atcgatggtt ttgtcaatgg cagagctgac agtagttgtg aggccaaggc
    23041 gttctaactc catcagacca gcaatagttg tgccaccagg gctacaaata gcgtcaatga
    23101 aatcgtgcgg actttgagaa ctggtcttga gattgctggc actagccaag actgtttgag
    23161 taacaatctc cagcgccttt gctttaggca tgccattctt gacgccagcc ttagccaaag
    23221 cctcaataaa gagataaata taggcaggac ttgaccctgc taaagcggta aaggtgtcaa
    23281 aatccttttc actaatatca aatgtgctac caaagctatc tgttaagtct cgaacacgtg
    23341 cttgtaattc ctgagacacc aaagcatttc ccgttaaagc ggtactgctt tggagaattt
    23401 gtgcattcat gtttggcatg atacgtagca gcggaaggtc ttgtcctacg aatgttgcta
    23461 gtcgttgaag ggaaatgcct gctgccatag atataatagg ctgtttgaag tgaagcggtt
    23521 tgagtaccgt ttcaaatagt tgaggcttga tgcctaaaat aacaagatca acctggtcaa
    23581 taaggtcttg gtgggacata gcataaggca gtgttaactg ctccgcaatt tccttggacc
    23641 gttctaaaga tgatcctgaa atgataagtt catggggtgt ttgtttaagg cctttgatga
    23701 tagcgctagc cattttgcca acaccaataa tgccaatttt cattctgttg ctccttgtgg
    23761 gatattatgg ttaatcaggc taatgctagt ttagtaacgt ttaatcaagt caacggttga
    23821 gcgatccagt tttttgataa tggcttgtaa gaaggcttgg gcttctacga aatcatccat
    23881 agcgtagagg gtttgatgag agtgaatgta gcgtgcacag actccgatgg ttgttgatgg
    23941 gacaccacca ttttgaaggt gtgcagcacc tgcatctgtg cctcccttgc cacaatagta
    24001 ttggaaattg acaccagcgt cctcagcagt agtcagtaag aagtcgcgca tatctttgag
    24061 catgacatgg cctgggtcgt agaaacgcaa caaggtgcca tccccgattg ttccaggatt
    24121 gccataaata tcaccagcag gcgaacaatc tactgcgaaa aagagttcag ggtcgaactt
    24181 ggtggttgag acgtgggctc cgcgcagacc aacttcttct tgaacgttag cacctgcaat
    24241 tagggtgttg ttaaggtctt gtccttttaa cgcttcaagc atttctgtta tcatgagaac
    24301 gccatagcga ttgtcccaag cttttgaaat aatatttttt tgattggctg ttaggatcgt
    24361 ttcagattga gggacaataa tatcacccgg tgtaatgcca aagctttcag cttctgcctt
    24421 atctgtaaag ccaccatcaa acacaatatc ttcgatatgt ggtagactag cagagccatt
    24481 tgccccacgg agaaaatgag gaggtaccga tcctgaaata aggggaataa cttggccagt
    24541 gcgtgtgtaa agggtaaacc gttgtgaact gacaacaagt gggttccaac caccgatacc
    24601 aaccacgcgt agcgttccgt caactttgat atcactgacc ataaaaccga cttcgtccat
    24661 gtgcgcagct actaaaatac ggggggcttt ttcagcttta ctatctctga taccaaaaat
    24721 gccaccaagc ccgtctgttt caacacggtc aaccagcggg gttattttgg tgcgtaggta
    24781 gtcacgaacg ctatgttcat agcccgcaat gccatccagt tcggtaactt ctttgatttt
    24841 tgagaataag tctgtcattt ttcatctcca tttaaaaggt ttcagaccag ttcaacgcgg
    24901 tcatctcctt atattctacc actttttaag taagaatggg tttcacagaa aagaaaaatc
    24961 ccagagacta gagatctctg gaagaagtgt ttgtcttttg gcgataatct gtttttttag
    25021 gcagcaagga aatcattaaa atgataaagc acaagctggt taataagaat agtagggcgc
    25081 ttaggatgcg aaaagactct tcactagcga aaagtaagga taatggagtc ataaggagaa
    25141 ccttccattt taggttaatc agtgattttt atagcgtggt taaattaata ataaaccaca
    25201 cctaatcaac gtacctttag tttagttgtt tttactattt ttgtcaatac ataacagtag
    25261 aaatctttag ttttcttatt tcagatcgtc caatttgatc tttttatgat aaaatgaggg
    25321 atatgagtaa aaagaaaata ggtatgattt caggtatctt tggatttagt ttagctattg
    25381 gcctaggaat agttatcaaa gattattgcc aagataggaa gcgccgacaa atgacaaggg
    25441 atttacgcac ctttttttca cctttaggac aaatcgaagt tttatatatc aatccttgtc
    25501 aggtcaaaca agattatatt tcaggtgggg ttgttatgtc aaacggcaaa cagtaccaat
    25561 tcacctatca cagtcgacaa attagttttg aggaggacaa gtgatgatca gaccgacttc
    25621 ttatgagtca ttagcggctc ttgttgaaaa agaagataag ctggtgttat tttttacggc
    25681 agactggtgt ccagactgtc aatttattta tcctattatg ccagaaattg aagcagaact
    25741 tacagatatg acttttgttt gtgttaatcg tgatcagttt atagaggtag cacaaaagtg
    25801 ggatattttt ggtattccta gttttgtcgt gattgaaaaa ggtcaggaag tagggcgtct
    25861 ggttaacaaa atgagaaaga caaaaactga gattatgcat tttttagctg cttatcaata
    25921 agaaaagagg aagaataaat gatttttgca tacaataaag aacaagttgg cgatgtcttg
    25981 atggttatct tacaagacac caaagatatc aaacgtcaag tagaacgaaa aggcaaagta
    26041 gcccgtgttt ttgcagaaga aagcggcaaa acccttgctt ggaatatctt tgaagcgtca
    26101 agcttgatta ctattgaagg caatggacag atttttttga cagacgagga ccttgcaaga
    26161 ttaaatgcag agcttgctaa ggaaggattt tcagaaaggc ttgaaccgac tgtgggacct
    26221 gtttttgtgg tcggtcaaat tgttgagatg gtggctcatc tagatagcga ccatcttaat
    26281 atctgtcaag tggctattgg tgaagatcaa acggttcaaa tcgtagcggg cgcaccaaat
    26341 gctgcgcttg gtttaaaaac gattgttgcc ttaccaggtg ctataatgcc aaatggtagt
    26401 ctgattttcc caggaaaatt gcgtggtgaa gaaagttatg gtatgatgtg ctctcctcgt
    26461 gagttagcct tgccaaatgc accgcaaaaa cgtggcatta ttgaacttga tgagtcagct
    26521 gtggtaggag aagcttttga cccagccaaa cattggaaag gttagaaaaa ccagctgaaa
    26581 taagactgta tttaactaaa tgaccagaaa aaaaggatta ggatccccta ttcctttttt
    26641 cttatgtttt caaaaacgtt taaaatgaaa cttattttac cacaattttt acgaataata
    26701 agataaggag gtcagtatgt ataataaagt gatagcaatc ggtcgtttgg tagctaaacc
    26761 agaattggta aaaacagcta cggataagca tgtagcacgt ctctctttag ctgttaatcg
    26821 aagatttaaa aatgcttctg gagagcgaga agctgatttt atttcagttg ttgtttgggg
    26881 aaagttagca gaaactctgg tttcttatgc tagcaaaggt agtttgatat ctattgatgg
    26941 cgaacttagg acccgcaagt atgataaaga tgggcaagtg cattatgtga cagaagttct
    27001 ctgccaatca tttcaactgc ttgaaagtcg tgctcagcgc gctatgagag aaaataatgt
    27061 tactaatgat ctagttgatt tagtcttaga agaagatact cttccctttt gatagaggat
    27121 aatgcttctc atcagggttc agctgattac tgaaatatat gatgaggtga tatccgagca
    27181 acaaaagtgt gactagatac ttctgataaa aataaaaaaa caccttacga gtatgaacaa
    27241 agtttcaagt gctagatgtt ggaactttgt tcatcatagg aatagtaggg tgttttttag
    27301 tgtattactt ctttttgttg ctggtgttaa ggtttacttg cttacaagcg gttatcaggc
    27361 cactagaact cttataataa atggagctct ttgagtttgg tgtcaatcat ttttaagaca
    27421 gtcgccaaat cttgtgggtt ttgaacgaaa tcaagggaat tgccatcaat tttcattttt
    27481 ggagagacct tgtagtcttc gtaccaggtt gggtattcac cgtggacttg atggtaatat
    27541 tgctcaagac taggattgcc gtcgacctgc tcaaaacttc ggccacgttt ttcaatgcgc
    27601 tcaagcattt tgtcaaaaga cacatcaatg tagatgagaa gatcagggcg ttttttaggc
    27661 atgccttcta gttcttctaa catgttggcg agaagttcct gatagatgtc aagttctgtc
    27721 ttagtgacat tgccattttt gtagttaagt tttaagaaga gttcgtcttc aaaaatagag
    27781 cggtcaagaa tgttgttatc ggcttgataa gcttctttga tggacttaaa ccgtttattc
    27841 aaaaaataaa tttgaagtaa aaaggcgtac tttttagggt cttggtagta gaggtctagc
    27901 acggggttat tatcaacagc ttcgtaaaaa acgtcagttc ctaaatgctc tccaagtgca
    27961 gcagcaagcg agctcttgcc agccccaatt gttccagcta gtacaatcaa catgcgtctc
    28021 cttctaatgt cattagcgta ctgtttcgaa gatggcttca acctcagcta gagtctcagc
    28081 gcgtgaaaca gcaccacgaa ctttggcagc acctgctgtt ccacgaagat agtgtggcgc
    28141 taagccccga aattcccgaa cagcaatggt ttcacctttg aggttaatca ggcgtttaag
    28201 gtggtctttg gcgatgtcta attttttggc aaagggaaga tctggcaatt cttgaccagt
    28261 ttcaaagaaa tgattgattt gggtaaaaag ataaggattg ttcatggctg cgcggccaat
    28321 catcacggca tctacaccga tttcttcaat cataaatttg gcgtcttgca ccgaacgcac
    28381 gtccccattt ccaataaagg gaatcttagt gatagcctta gaaactcggg ctaatgtctc
    28441 atggtcgcag gttcctgtat acatttgttc acgggtacga ccgtgcatgg caagagcaga
    28501 tacacctgcc gactcagcgg caagggcatt ctcaacggca agtgaactat ccgcccaacc
    28561 tgtacgcatt ttgacagtca gtggaatatc taaaacagac gtgacttcct tgacaatgtg
    28621 gtaaatctta tcagggtcac ggagccactt ggcaccagcc tcgtttttga caactttatt
    28681 aacagggcag cccatattaa tatcaacaat atcggccttg gtattggttt gaatgaaatc
    28741 agctgcacgt ttcaaccctt ctgcatcacc accaaataat tgaatggaca tagggtgctc
    28801 attctcgtca atatggagca tatggagggt cttttcgttg ttgtagagca gccctttttc
    28861 ggaaatcatt tccataacca ccaaaccagc accaaattct ttggcgatgg tacgaaaggc
    28921 agagttggtc acaccagcca tgggagcaag gacagtgcgg tgagggattt ccacatcacc
    28981 aatcctaaag gaagaattaa gctttatcat tgataatggc ctccaaatca ctttcattaa
    29041 attggtactt ggtaccacaa aattggcaaa caatctcagc acccttatct tcgtcaatca
    29101 tggcctgtaa atctgctttt ggcaaagtca ttagggctgc ttcaaaacgt tctctagaac
    29161 agtcacactg aaaactcaga ggttcttcag aaagacgctt gtaaggttca tctccataga
    29221 tggcctccaa aagggcatcc acatgatttt tggaagctag taggtgagaa atggcaggca
    29281 tttcttgcag gcgtttttca tagcgagcaa cctcttcttc agaagctcct ggtagcacct
    29341 gcaccataaa gccaccagca accttgacct tatcattttc atctaaaagc acattaagtc
    29401 cgattgcaga tggtgtttgc tctgattcgg ttaagtagta ggcaaaatcc tcaccaattt
    29461 ccccagtaat aagaggcgtt gttgaggtgt aagggttacc tgtgccataa tcaataatcg
    29521 tcacaaaatg accattcccc ataaaaggcc caacaaggac ttcacctgtt gcggtctttt
    29581 taatatcgac accagtattt tgaatgtaac ctttcacatg tcctttggta tctgccacag
    29641 agataatgtg tccaaaagaa gaatcaccaa tcactttgac ggtgattcta ctatcgcctt
    29701 tttgattagc cgccaaaatc tggttggcga tcaaggtacg gccaagcgca acagtagacg
    29761 atgacagcgt gttgtgtttt tcttgagcta atgcaactgt ctcagtgcta tcaaggacat
    29821 aagccctaaa agaccctgat tgtgctatcg atttaattat tttatccata acttagatta
    29881 tagcataaag tgtaaaactt gtattgacag gattgcttga aaaatcaata cagatagaca
    29941 gtaaaaaatc ccagagaatg actcgggatt aagttaaggt gtggtctaaa gtcgcgcaat
    30001 gtggtgacat ggcgcttatg ttaattgctt ggttaaatca gcttggaatt tttcctcttt
    30061 aacttgatac atcaattctt ggatagacag aatcgattca tcaaaagata tttcagcaac
    30121 agcaattcct tttgtaagtt catggtgaac aaaaggaatc agttgactgt gagtgatgac
    30181 caaatctggc tttaaatcag gaatttgata aacattatct tgcaatagat aataggaatg
    30241 aaaatcaatg cttttatccg agaaatacct tgggaaagaa tctgttagga gatgagcatt
    30301 gataaaatta ctggctacga aaacaacaac taaaggaggt tggatatttc ttagaatttg
    30361 ctcgacatag tggcaaaaaa gatgaaaatg cttatggttc aagtaacgct taccaggaag
    30421 tttggccatc cactcttcga caattaactt taaggacgta tagagttttt ggtttccttt
    30481 atagtacgga gaaacgaata agttggtctc aggaataaaa tgttgcagat taaacaagaa
    30541 tgattttgaa aaaaacataa gagcttttac taaactagcc ttttgctctt ttaggttagg
    30601 taaaagagtg atgataggat ttaaaagcag gcgaaaagta tcattttctt caaaaagttg
    30661 acaacattgt ctgatatgct caggtgtcca ttgtaagctc gcaaaagaat tattagcggt
    30721 gatataaatt aaatagaggt agtccaaatc tcctgctgaa aagtttagtt ggcagtaagt
    30781 ttcgataata tcacggctac tttttttcaa agaatcgtag acaaaaagtt tttttaattg
    30841 ttgaaaaatt ctggtttggg gaatagttac cgaaaattga tgccgcttcc acgataaagc
    30901 taataaaatg tcatagaaag aaaacgattc cgataaccaa ggagaagttt taaggtgggt
    30961 ggaactatgg gataaaaagc tatgaataat gtttttgtct tgctgcgtca agtcataaac
    31021 tttaatgcca aacttactat atagcagagc gatgaggtaa cggatacgat attcctcacc
    31081 gacaatcttg ttcttagaga gttttaattc aaagtttctt aataaaggaa tcaatgcttc
    31141 gcgcatccga taagctgagg agtttgataa aaaaatgact tcttgcaaaa tccgtaaggg
    31201 gacgagagtg ggaaccattt tttattaaaa acgctagtaa ttgtaagaca ttagatgatg
    31261 catagagttg gtaaagataa gtttctttag aaggatgtgt aaattggcaa gatatcatcc
    31321 ttttttggat ggtcatagac agactatcag ggaaaaaggc attcagctcc tcacaataat
    31381 ggtttagttg taaaaaggtt aagccagttt tttctgctac ctcagttatt ggcaaagaag
    31441 atgtcttaaa aaacaagaca actaactgac attttgattc gattgatgat tccaagtatt
    31501 tttctatcaa ggaaaggggt cctctcttcc tacacttaga aagccaaata agtctttgat
    31561 aagtgtattc tcctatttca attctatcta agctaaaaat agaaatcaaa tctaatggtt
    31621 tttggtgaaa agtcgaa
//
LOCUS       DAUDYX010000018        31251 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.018, whole genome shotgun sequence.
ACCESSION   DAUDYX010000018 DAUDYX010000000
VERSION     DAUDYX010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31251
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.018"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..281
                     /locus_tag="VHD02_000820"
     CDS             <1..281
                     /locus_tag="VHD02_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ5497442.1"
                     /translation="ILADLGYLSRELKNNLEQKGYQLWTPLRQNMTGAKQHNHWRVMA
                     MRRTIETRFSELCSLFDIEHTLTRGVTGLQLRIEQIILAYNLKYVEFN"
     gene            complement(453..1730)
                     /locus_tag="VHD02_000821"
     CDS             complement(453..1730)
                     /locus_tag="VHD02_000821"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEQ5497443.1"
                     /translation="MTKTNLNWSGFSKKTFEERLQLIEKFKLLNAENLNQLKTDVLLP
                     IQTANQMTENVLGRLALPFSIAPDFLVNGSTYQMPFVTEEPSVVAAASFAAKLIKRSG
                     GFKAQTLNRQMIGQIVLYDIDQIDNAKAAILHKTKKLIALANKAYPSIVKRGGGARTI
                     HLEEKGEFLIFYLTVDTQEAMGANMVNTMMEALVPDLTRLSKGHCLMAILSNYATESL
                     VTTSCEIPVRLLDRDKTKSLQLAQKIELASRLAQVDPYRATTHNKGIFNGIDAVVIAT
                     GNDWRAIEAGAHAYASRNGSYQGLSQWHFDQDKQVLLGRMTLPMPIASKGGSIGLNPT
                     VSIAHDLLNQPDAKTLAQLIASVGLAQNFAALKALTSSGIQAGHMKLHAKSLALLAGA
                     TQDEIAPLVNALLADKPINLEKAHFYLSQLRQS"
     gene            complement(1717..2892)
                     /locus_tag="VHD02_000822"
     CDS             complement(1717..2892)
                     /locus_tag="VHD02_000822"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEQ5497444.1"
                     /translation="MTIGIDKIGFATSQYVLKLEDLALARQVDPAKFSQGLLIESFSV
                     APITEDIITLAASAADQILTDEDRSKIDMVILATESSTDQSKASAIYVHHLVGIQPFA
                     RSFEVKQACYSATAALDYAKLHVASKPDSRVLVIASDIARYGVGSPGESTQGSGSIAL
                     LVTANPRILALNKDNVAQTRDIMDFWRPNYSFTPYVDGIYSTKQYLNCLETTWQAYQK
                     RENLQLSDFAAICFHIPFPKLALKGLNNIMDNTVPPEHREKLIEAFQASITYSKQIGN
                     IYTGSLYLGLLSLLENSKVLQSGDKIGFFSYGSGAVSEFYSGQLVAGYDKMLMTNRQA
                     LLDQRTRLSVSKYEDLFYEQVQLDDNGNANFDIYLTGKFALTAIKEHRRIYHTNDKN"
     gene            3101..3940
                     /locus_tag="VHD02_000823"
     CDS             3101..3940
                     /locus_tag="VHD02_000823"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEQ5497445.1"
                     /translation="MTKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAF
                     AEYDLAKGEFPITTLRPIPIKSAIKELFWIYQDQSNSLDVLEAKYNVHYWNEWEVDQT
                     RTIGQRYGAVVKKHDIISKILKQLAENPWNRRNVISLWDYEAFEETKGLLPCAFQIMF
                     DVRRVGEDLYLDASLTQRSNDILVAHHINAMQYVALQMMFAKHFGWKIGKFFYFVNNL
                     HIYDNQFDQAQELLKRQPVASQPKLVLNVPDGTNFFDIKPDDFELQNYDPVKPQLHFD
                     LAI"
     gene            4020..4517
                     /locus_tag="VHD02_000824"
     CDS             4020..4517
                     /locus_tag="VHD02_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEQ5497446.1"
                     /translation="MTKEIIAIWAEDEAGLIGVAGKLPWYLPKELEHFKKTTLHQAIL
                     MGRVTFEGMNCKRLPQRQTLVMTRNRDYQVDEVLTMTSIEKVLEWYHAQDKTLYIIGG
                     NKVLEAFNGYFDRIIKTVIHHRFKGDTYRPNLDFSHFTQESQTFYARDAKNPYDFTVT
                     ALKHK"
     gene            4537..4707
                     /locus_tag="VHD02_000825"
     CDS             4537..4707
                     /locus_tag="VHD02_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497447.1"
                     /translation="MQRSIFGVFTAFLCVICVLCAIPAFKKNRYGLGSLFLLNAFTNL
                     VNSIHAFYGTLF"
     gene            4837..6066
                     /gene="clpX"
                     /locus_tag="VHD02_000826"
     CDS             4837..6066
                     /gene="clpX"
                     /locus_tag="VHD02_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEQ5497448.1"
                     /translation="MAGSRTNDIKVYCSFCGKSQDDVKKIIAGNNVFICNECVALSQE
                     IIKEELSEEVLADLTEVPKPKELLDVLNQYVVGQDRAKRALSVAVYNHYKRVSFTESR
                     DDDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTV
                     ASVPPQGGRKHPNQEMIQIDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNSRKID
                     DNASYMQEIISEDIQKFGLIPEFIGRLPVVAALEQLKTSDLIRILTEPRNALVKQYQA
                     LLSYDGVELEFDKAALEAIATKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVR
                     ITKAAVEGKSKPVLETA"
     gene            6076..6675
                     /locus_tag="VHD02_000827"
     CDS             6076..6675
                     /locus_tag="VHD02_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEQ5497449.1"
                     /translation="MAEEQVLNTHNASILLSAANKSHYPQDDLPEIALAGRSNVGKSS
                     FINTILGRKNLARTSSKPGKTQLLNFFNIDDKLRFVDVPGYGYAKVSKSERAKWGKMI
                     EEYLTTRDNLRAVVSLVDLRHAPSKEDIQMYDFLKYYDIPVIVVATKADKIPRGKWNK
                     HESVVKKALNFDKSDTFIVFSSVERIGIDDSWDAILEQV"
     gene            6823..7566
                     /locus_tag="VHD02_000828"
     CDS             6823..7566
                     /locus_tag="VHD02_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497450.1"
                     /translation="MTQVAQGRSKGKSLLCHQCNALFVFIIFLILLAVLFGVRYRNSS
                     IEGIWRTTSIDQKLGDDFAKRLTGLHQSPLIDDSLLTSSQMILTVKNNNVDLSFSVQV
                     ERDIFVKRLAAYHQNELLKTLKENHLVVGDLSSKERQIIENSMPASHELEMILDQAFE
                     KLASQIGGKYNQKTGHLSAVVLKGKVNRILHTIDIKEEVAAGHTSFSKGLLTPNGYFD
                     YTRFGKKLELLGDEKIIFKKALKKSPSSV"
     gene            complement(7624..9723)
                     /locus_tag="VHD02_000829"
     CDS             complement(7624..9723)
                     /locus_tag="VHD02_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEQ5497451.1"
                     /translation="MTHFSGKDPFVNMDDIFNQLMANMGGYRSENPRYLVNGREITPE
                     EFQHYRQTGQLPVATTKATNSQMLTPKADSVLTQLGTNLTQEARQGHLDPVIGRNKEI
                     QDTAEILARRTKNNPVLVGDAGVGKTAVIEGLAQAIVNGDVPAAIKNKEIVSIDISSL
                     EAGTQYRGSFEETIQNLIQEVKEAGNIILFFDEIHQIVGAGATSSDSGSKGLADILKP
                     ALSRGELTLIGATTQDEYRNTILKNAALARRFNEVKVNAPSAEDTFHILMGIRNLYEQ
                     HHHITLPDNVLKAAVDYSIQYIPHRSLPDKAIDLLDMTAAHLAAQHPVTDLKTLETEI
                     AKQKESQEKAVAKEDFEKALAAKTRIETLQKQIEQHNQSQNVTATVNDIAESVERLTG
                     IPVSNMGTNDLERLKGISSRLKSHVIGQDEAVAAVARAIRRNRAGFDDGNRPIGSFLF
                     VGPTGVGKTELAKQLALDLFGSKDAIIRLDMSEYNDRTAVSKLIGTTAGYVGYDDNNN
                     TLTERVRRNPYAIVLLDEIEKADPQIITLLLQVLDDGRLTDGQGNTINFKNTVIIATS
                     NAGFGQQDTETSGSNIMDRIAPYFRPEFLNRFNSIIKFNHLQKENLEEIVDLMLAEVN
                     QTTAKKGISLTIADDAKAHLIDLGYNHAMGARPLRRIIEQEIRDRITDYYLDHPEVKK
                     LQAILKEGQLVIRQKDQ"
     gene            10101..10784
                     /gene="rpiA"
                     /locus_tag="VHD02_000830"
     CDS             10101..10784
                     /gene="rpiA"
                     /locus_tag="VHD02_000830"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEQ5497452.1"
                     /translation="MEALKKIAGVTAAQYVTDGMTIGLGTGSTAYYFVEEIGRRVKQE
                     GLQVVGVTTSSVTSKQAEGLGIPLKSIDDIDSIDLTVDGADEVDKDFNGIKGGGAALL
                     MEKIVATPTKEYIWVVDASKMVEHLGAFKLPVEVVQYGADRLFRVFEKAGYKPSFRMK
                     GDSRLVTDMQNYIIDLDLGCIKDPVAFGHLLDGTVGVVEHGLFNGMVDKVIVASKDGV
                     IVLEAPKAG"
     gene            10861..12072
                     /locus_tag="VHD02_000831"
     CDS             10861..12072
                     /locus_tag="VHD02_000831"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEQ5497453.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDADKFFNAGVADTDSDTLGHISE
                     TAGLSVPNMAKIGLGNISRPIPLKTIPTEDNPTGYVTKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKIIREANKPYSGTAVIDDFGPRQMETGELIV
                     YTSADPVLQIAAHEDIIPVEELYKICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFQDTVLNKLADAGVPTYAVGKINDIFNGSGITNDMGHNKSNSHGIDTL
                     IKTLQLPEFTKGFSFTNLVDFDANFGHRRDPEGYRDCLHEFDNRLPEIFANMKEDDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSASFTGNGLIPQGHFADISATVAENFGVDTA
                     MIGESFLGHLK"
     gene            12091..12531
                     /gene="arsC"
                     /locus_tag="VHD02_000832"
     CDS             12091..12531
                     /gene="arsC"
                     /locus_tag="VHD02_000832"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922177.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEQ5497454.1"
                     /translation="MEEITIYHNPNCGTSRNVLAMIRHAGIEPTIIEYLQTPPNRETL
                     IELLQSMGISARELLRTNVPEFEAHGLANQAVAEKDVINAMLADPILINRPIVVTRKG
                     VKLCRPSETLLDILPVPLPSPYMKEDGESVNPIEVKGEKDVIDD"
     gene            12515..13324
                     /locus_tag="VHD02_000833"
     CDS             12515..13324
                     /locus_tag="VHD02_000833"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEQ5497455.1"
                     /translation="MSLMTKINETKDFLVTKGIETPEFGLILGSGLGELAEEVENAIV
                     IDYADIPNWGKSTVVGHAGKLVYGDLAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKA
                     LGCEGVLVTNAAGGIGYGPGTLMAITDHINMTGNNPLIGENLDEFGPRFPDMSDAYTK
                     VYRNKAHEMAEKMNIKLEDGVYMGLTGPTYETPAEIRAFKVLGADAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTQHIKEDFKGLVKAILAEL"
     gene            13987..14700
                     /gene="deoD"
                     /locus_tag="VHD02_000834"
     CDS             13987..14700
                     /gene="deoD"
                     /locus_tag="VHD02_000834"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEQ5497456.1"
                     /translation="MSIHISAKKGDIADKILLPGDPLRAKFIAENFLEDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAIDPEVHVRE
                     LVLAQAAATNSNIIRNDFPEFDFPQIADFGLLDKAYHIAREMGVTTHVGNVLSSDVFY
                     TNMPERNMALGKLGVKAIEMEAAALYYLAAQHHVKALGIMTISDNLNDPTEDTTAEER
                     QTTFTDMMKVGLETLIAND"
     gene            14693..15481
                     /locus_tag="VHD02_000835"
     CDS             14693..15481
                     /locus_tag="VHD02_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEQ5497457.1"
                     /translation="MTNNQTLDILLDVYAYNHAFRIAKALPNIPKTALYLLEMLKERR
                     ELNLAFLAEHAAENRIIEDQYHCSLWLNQSLEDEQIANYILDLEVKVKNGAIIDFVRS
                     VSPILYRLFLRLITSEIPNFKAYIFDTKNDQYDTWHFQAMLESDHEVFKAYLSQKQSR
                     NVTTKSLADVLTLTSLPQEIKDLVFLLRHFEKAVRNPLAHLIKPFDEEELHRTTHFSS
                     QAFLENIITLATFSGVIYRCEPFYFDDMNAIIKKELSLWRQSIV"
     gene            complement(15561..16466)
                     /locus_tag="VHD02_000836"
     CDS             complement(15561..16466)
                     /locus_tag="VHD02_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ5497458.1"
                     /translation="MRIQQLHYIIKIVECGSMNEAAKQLFITQPSLSNAVKDLEMEMG
                     ITIFNRNPKGITLTKDGVEFLSYARQIIEQTSLLEDRYKNHNTGRELFSVSSQHYAFV
                     VNAFVSLLKRTDMTRYELFLRETRTWEIIDDVKNFRSEIGVLFINDYNRDVLTKLFDD
                     NHLTASPLFKAQPHIFVSKSNPLATKSLLSMDDLSDFPYLSYDQGIHNSFYFSEEMMS
                     QMPHSKSIVVSDRATLFNLMIGLDGYTVASGILNSNLNGDQIVAIPLDVPDEIDIVFI
                     KHEKANLSKMGERFIEYLLEEVTFD"
     gene            16688..17224
                     /locus_tag="VHD02_000837"
     CDS             16688..17224
                     /locus_tag="VHD02_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497459.1"
                     /translation="MNVTEQNSHQILIQKLLVSIHYLTLFRDELKLVERTPSILGGEF
                     PAHLVQSELGDIVAAIDTLDMQQRLIESTFWYEESAFKLMNKTLDIVDNWIKGVDHLI
                     DLCQSKEVFQIIIGDKRIRVFGVLSDVFSSLKVSALSLKEAPIPDVLYEHIQRVNLEE
                     ETFIKYYESPKVSTGEFE"
     gene            17501..18193
                     /gene="pyrF"
                     /locus_tag="VHD02_000838"
     CDS             17501..18193
                     /gene="pyrF"
                     /locus_tag="VHD02_000838"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEQ5497460.1"
                     /translation="MKEERPIIALDFSSFEETKAFLDLFPAEEKLYVKIGMELYYAQG
                     PDIVRSIKSLGHNVFLDLKLHDIPNTVRAAMAVLKELDIDMATVHAAGGVEMLKAARE
                     GLGQGPTLIAVTQLTSTSEDQMRGDQNIQTSLLESVLHYSKGAAKAQLDGVVCSAQEV
                     EAIKAVTPTGFTCLTPGIRPKGSNIGDQKRVMTPNQARRIGSDYIVVGRPITQAKDPV
                     AAYQAIKAEWAG"
     gene            18251..18880
                     /locus_tag="VHD02_000839"
     CDS             18251..18880
                     /locus_tag="VHD02_000839"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEQ5497461.1"
                     /translation="MTLASQIATQLLDIKAVYLKPEDPFTWASGIKSPIYTDNRVTLS
                     YPKTRDLIENGFVETIRAHFPEVEVIAGTATAGIPHGAIIADKMTLPFVYIRSKPKDH
                     GAGNQIEGRVLKGQKMVIIEDLISTGGSVLDAAAAASREGADVLGVVAIFTYELPKAS
                     QNFKEAGIKLITLSNYTELIAVAKLQGYITNDGLHLLKKFKEDQVNWQQ"
     gene            19077..20531
                     /locus_tag="VHD02_000840"
     CDS             19077..20531
                     /locus_tag="VHD02_000840"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEQ5497462.1"
                     /translation="MTYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSE
                     RFEAALEEAKQRDFSGLPFAGVPLFLKDLGQELKGHSSTSGSRLFKEYQATKTDLFVK
                     RLEALGFIILGRSNTPEFGFKNISDSSLHGPVNLPKDNTRNAGGSSGGAAALVSSGIS
                     ALATASDGGGSIRIPASFNGLIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTR
                     NLLYYLQMEQMESPFPLATLTKDSIYQSLQRPLTIAFYQRLSDGSPVSLDTAKALKQA
                     VTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIEDTFGRPMTKDDMET
                     MTWAIYQSGKDIPAWRYSQVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVP
                     DSKLMANLAQAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEG
                     LPMGIQLIAAKGREDLLLGIAEQFEASGLLKIPQ"
     gene            20649..21512
                     /locus_tag="VHD02_000841"
     CDS             20649..21512
                     /locus_tag="VHD02_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEQ5497463.1"
                     /translation="MKKSVTLLSIGLASLLLAACAPHKSQKSSWDTIKEKGVLKVATP
                     GTYQPTSFYNDNNELVGYEVDMVKEIGKRLNIKVKFVETGFDQAFTSVDSGRVDISLN
                     NFDITPKRQKKYNISTPYKYGVGGMIVRADGSSNMAKKDLSDWKGKKAAGASGTEYMK
                     VAQKQGAELVTYDNVTGDVYLNDVANGRTDFIPNDYPAQKLFVDYMLSQNPNLNVKMS
                     DVQYNPTEQGIVMNKKDDSLKKKIDAVIKDMIKDGSLKKISETYYAGQDLTKPFGKDK
                     KIPVIDTKDVN"
     gene            21541..22191
                     /locus_tag="VHD02_000842"
     CDS             21541..22191
                     /locus_tag="VHD02_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ5497464.1"
                     /translation="MINIPLMKDSLGFVLSGLPYTLGISLLSFFTGLFLGLGLALLGR
                     SRQPLIHYLVRAYISIMRGVPMIVVLFVLYFGLPYYGLELPALLCAYLGFSMVSAAYI
                     SEVFRSSIEAIDKGQWEAAKALGLPYALMVKKIILPQAFRIAVPPLGNVIIDMVKSSS
                     LAAMITVPDIFQNAKIIGGREWDYMSMYILVAFIYWIIAFLLERYQEFLENKLALV"
     gene            22324..22977
                     /locus_tag="VHD02_000843"
     CDS             22324..22977
                     /locus_tag="VHD02_000843"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEQ5497465.1"
                     /translation="MAHSIWHEKIKSFLPEHYYGRINHFLDEAYASGLVYPPRENVFK
                     ALQVTPLEETKVLILGQDPYHGPKQAQGLSFSVPEEISAPPSLINILKELADDIGPRD
                     HHDLSTWASQGVLLLNACLTVPAGQANGHAGLIWEPFTDAVIKVLNEKDSPVVFILWG
                     AYARKKKAFITNPKHHIIESPHPSPLSSYRGFFGSKPFSRTNAILEKEGMTGVDWLK"
     gene            23109..24377
                     /locus_tag="VHD02_000844"
     CDS             23109..24377
                     /locus_tag="VHD02_000844"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEQ5497466.1"
                     /translation="MILIKNGRVMDPKSQRDQVADVLIDGKQIVKIASAIECQEAQVI
                     DASGLIVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGVTTVVMMANTNPVISDVE
                     TLQEVLASAAKEKIHIYTNASVTQAFNGKDVTDFKALLEAGAVSFSDDGIPLESSKVL
                     KEAFDLANANQTFISLHEEDPQLNGVLGFNEGIAEEHFHFCGATGVAEYSMIARDVMI
                     AYDRQAHVHIQHLSKAESVQVVAFAQQLGAKVTAEVSPQHFSTTEDLLLTAGTSAKMN
                     PPLRTQRDRLAVIEGLKSGVITVIATDHAPHHKDEKAVDDMTKAPSGMTGLETSLSLG
                     LTHLVEPGHLTLMSLLEKMTLNPALLYGFDAGYLAENGPADLVIFADKQERLITENFA
                     SKASNSPFIGNKLKGVVKYTIADGEVVYPN"
     gene            complement(24435..25076)
                     /gene="plsY"
                     /locus_tag="VHD02_000845"
     CDS             complement(24435..25076)
                     /gene="plsY"
                     /locus_tag="VHD02_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEQ5497467.1"
                     /translation="MKLLLFITIAYLLGSIPTGLWIGQYFYHINLREHGSGNTGTTNT
                     FRILGVKAGTATLAIDMFKGTLSILLPIIFGMTSISSIAIGFFAVLGHTFPIFANFKG
                     GKAVATSAGVLLGFAPLYLFFLASIFVLVLYLFSMISLASVVSAIVGVLSVLTFPAIH
                     FLLPNYDYFLTFIVILLAFIIIIRHKDNISRIKHHTENLIPWGLNLSKQVPKK"
     gene            25211..27160
                     /gene="parE"
                     /locus_tag="VHD02_000846"
     CDS             25211..27160
                     /gene="parE"
                     /locus_tag="VHD02_000846"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEQ5497468.1"
                     /translation="MTKKEITINNYNDDAIQVLEGLDAVRKRPGMYIGSTDATGLHHL
                     IWEIVDNAVDEALSGFGDDIKVVINKDGSVSVADSGRGMPTGQHAMGIPTVQVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSAWLEVEITRDGSVYRQRFENGGKPVTTLK
                     KVGTAPKSKSGTVVTFMPDDKIFSTIDFKFNTISERLKESAFLLKNVKMSLTDLRGDD
                     PIIEEFHYENGVQDFVEYLNEDKETLTPVIYMEGQDQDFQVEVALQYNDGFSDNILSF
                     VNNVRTKDGGSHETGLKSAITKAMNDYARKTNLLKEKDKNLEGSDYREGLSAVLSILV
                     PEQHLQFEGQTKDKLGSPLARPIVESIVSEKLTFFLLENGEVASHLVRKAIKARDARE
                     AARKARDDSRNGKKNKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTEKAKMADILKNEEINTMVYTIGAGVGADFNLEDINYDKIIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVETGHVYIALPPLYKMSKGKGKTEKVSYAWTDGEL
                     EDLRKEFGKGAILQRYKGLGEMNANQLWETTMDPETRTLIRVTIDDLARAERRVSVLM
                     GDKAAPRRQWIEDNVKFTLEENTVF"
     gene            27251..29710
                     /gene="parC"
                     /locus_tag="VHD02_000847"
     CDS             27251..29710
                     /gene="parC"
                     /locus_tag="VHD02_000847"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEQ5497469.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQDWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEVRLSEIAGYLLQDIEKNTVSFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSSGISAGYATDIPPHNLSEVIDAVVYMIDHPKASLEKLMEFLPGPDFPTGGI
                     IQGADEIKKAYETGKGRVVVRSRTEIEELKGGKQQIIVTEIPYEVNKAVLVKKIDDVR
                     VNNKVPGIVEVRDESDRTGLRIAIELKKDADSQTILNYLLKYTDLQVNYNFNMVAIDH
                     FTPRQVGLQKILSSYISHRKDIIIERSKFDKAKAEKRLHIVEGLIRVLSILDEIIALI
                     RSSDNKADAKENLKVSYDFSEEQAEAIVTLQLYRLTNTDIVTLQNEENDLRDLITTLS
                     AIIGDEATMYNVMKRELREVKKKFANPRLSELQAESQIIEIDTASLIAEEETFVSVTR
                     GGYLKRTSPRSFNASSLEEVGKRDDDELIFVKQAKTTEHLLLFTTLGNVIYRPIHELT
                     DLRWKDIGEHLSQTISNFATEEEILYADIVTSFDQGLYVAVTQNGFIKRFDRKELSPW
                     RTYKSKSTKYVKLKDDKDRVVTLSPVIMEDLLLVTKNGYALRFSSQEVPIQGLKSAGV
                     KGINLKNDDSLASAFAVTSNSFFVLTQRGSLKRMAVDDIPQTSRANRGLLVLRELKTK
                     PHRVFLAGGVQSDTSAEQFDLFTDIPEEETNQQMLEVISKTGQTYEIALETLSLSERT
                     SNGSFISDTISDQEVLVARTR"
     gene            29788..30855
                     /locus_tag="VHD02_000848"
     CDS             29788..30855
                     /locus_tag="VHD02_000848"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEQ5497470.1"
                     /translation="MKKSYNRKKQKRGTMMTIAIDWDNLGFEYHKLPFRYISYYKNGQ
                     WDKGQLTEDATLHISESAPALHYGQQAFEGLKAYRTKDGSIQLFRPDRNAARLQATAD
                     RLLMPQVSTEQFIDAAKQVVKANEDFVPPYGTGATLYLRPLLIGVGDIIGVKPAEEYI
                     FTIFAMPVGNYFKGGLAPTNFIVSEAFDRAAPYGTGAAKVGGNYAGSLLPGKAAKSAG
                     FSDVIYLDPATHTKIEEVGAANFFGITANNEFVTPLSPSILPSITKYSLLQLAEERLG
                     MTVIEGDVPINELDKFVEAGACGTAAVISPIGGIQYKDNLHVFYSETEVGPVTRRLYD
                     ELVGIQFGDIEAPEGWIVKVD"
     gene            30919..31149
                     /locus_tag="VHD02_000849"
     CDS             30919..31149
                     /locus_tag="VHD02_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEQ5497471.1"
                     /translation="MSKKDKKIEIQLIDHKVMVNETKIDGYQLQIGKRVVGEIAELDE
                     KFAVLKNDGVDCFFKTLEQAVENIIENYNLNH"
BASE COUNT         9793 a         5561 c         6464 g         9433 t
ORIGIN      
        1 tcattttagc agacttgggc taccttagcc gtgagctgaa aaataatctg gaacaaaaag
       61 gctatcagct atggacaccc ttgcgtcaaa atatgacagg ggctaaacag cataatcatt
      121 ggagagtgat ggctatgaga cgaactattg aaactcgatt ttcagaactt tgttcccttt
      181 ttgatataga acacacactg actagaggag ttacaggatt acaattgaga atcgaacaaa
      241 ttatccttgc ttataatctg aaatacgttg agtttaacta gcaccacgag taatatttat
      301 aaatacctca tcaaactcag aatagagtag atgagtaaat agagtatgtt atggagacta
      361 tttctaagac ttgtatgtat agaaacaaag attaaacacc catgtaaacc aattgtttac
      421 atgggtgttt gccttatatt agttgtttaa atctaagact gtcttagctg ggataagtaa
      481 aaatgtgctt tttctagatt tattggttta tcagctagta aagcattaac taaaggagca
      541 atttcgtctt gggttgcccc cgccaaaaga gctaatgatt tcgcatgtag tttcatgtga
      601 ccagcttgga tgccagatga ggtcagagct tttagtgcag caaagttttg agctaacccc
      661 acagatgcaa tcaattgggc taatgttttg gcatcaggtt gattaagaag atcatgtgcg
      721 atagaaacag tagggttaag cccgatagat ccccccttac tagcaatagg catagggagg
      781 gtcattcggc caagcagaac ttgtttatct tgatcaaaat gccactgact aagtccttga
      841 tagctaccat ttcttgaggc ataagcatgg gcccctgctt caatagcacg ccagtcattt
      901 cctgtggcta ttaccactgc atcgatacca ttaaaaatac ctttattatg agtagtagcc
      961 cggtaaggat ctacttgagc tagtcggctg gctagctcta ttttttgagc taactgtagg
     1021 gattttgttt tatcgcgatc taaaaggcgc acgggaatct cacaactagt agtaacaagc
     1081 gactctgttg cgtaattaga taaaatcgcc attagacaat gccccttaga cagtcttgtt
     1141 aaatcaggaa caagagcttc catcatagta ttgaccatat ttgctcccat agcttcttgg
     1201 gtatcaacag tcagatagaa aatcaaaaac tctccttttt cttccaaatg aatggttcta
     1261 gcgcctccac ctcttttaac aatggaagga taagctttat ttgccaatgc aattagcttt
     1321 tttgttttat gaaggatggc ggctttagcg ttatctattt ggtcgatatc gtaaagaaca
     1381 atttgaccaa tcatttgtcg gtttagggtt tgagctttaa aaccacctga gcgtttgatt
     1441 agttttgctg cgaaagatgc tgcagcaaca acagaaggtt cttccgtgac aaaaggcatc
     1501 tgataagttg aaccgttgac aagaaaatca ggagctatgc taaagggcaa agccaatcgt
     1561 cctaagacat tttcagtcat ttgattagct gtttggatag gcaaaagaac gtctgttttg
     1621 agttgattta agttttcagc attaagtagt ttaaattttt cgataagttg gaggcgttct
     1681 tcgaatgttt tctttgaaaa accgctccag ttaagattag tttttgtcat tggtatgata
     1741 gatccttcga tgctccttga tggctgttag agcaaatttt ccagttaagt aaatgtcaaa
     1801 attggcatta ccattatcat ctaattggac ttgttcgtag aaaaggtctt cgtatttaga
     1861 aacggaaaga cgtgttcgtt gatccagtaa agcttgtcga ttagtcatta acattttatc
     1921 gtacccagca actaactgac cggaataaaa ttcacttacg gcaccggaac catagctaaa
     1981 aaaaccaatt ttatctccag attgtaatac tttactattt tcaagtaaag ataacaatcc
     2041 tagataaagt gagccagtat aaatatttcc aatttgtttg ctataagtaa tagaagcttg
     2101 aaaggcttct attagttttt ctctgtgttc aggaggtact gtgttatcca taatgttatt
     2161 tagacctttt agggctaact tgggaaatgg aatatggaaa caaattgcag caaaatcaga
     2221 taactgaagg ttttctcttt tctgataagc ttgccacgtg gtttctaagc aattgagata
     2281 ttgcttggta gagtaaatac catcaacata aggtgtgaaa ctatagttag gcctccaaaa
     2341 gtccataata tccctagttt gagccacatt atctttattt agagcaagaa tacgagggtt
     2401 agcagttacc aaaagagcaa tactaccaga gccttgagtt gattcgccag gagatcctac
     2461 accgtatcta gcaatatcac tagcaataac aaggacacga gaatctggct tagaagccac
     2521 atgcagttta gcatagtcta gagcagcagt tgcgctatag caggcttgtt ttacttcaaa
     2581 ggaacgtgca aaaggctgga taccaactaa gtgatgtaca tagatagctg atgcctttga
     2641 ctgatcagta ctcgattcag ttgccaaaat aaccatatca atcttagatc gatcttcgtc
     2701 ggttaaaatt tggtctgctg cagaagcagc taaagtaata atgtcttcag tgattggtgc
     2761 cacactaaaa gactcaatga gtagcccttg actaaatttt gctggatcca cttggcgtgc
     2821 aagcgctaaa tcttccaatt tcaagacata ttgactggtt gcaaaaccaa tcttatcaat
     2881 tccaattgtc atatttgtcc ttcttttagt tatttttata gtcttaccca ttctatcaca
     2941 ttttactgca aaattagact gataaagtta tttgttttat cctttaatag ttaaaatgtt
     3001 catgaccaca tgaagttttt cgaatgttta tttattctgg tattttttgg taaaattagt
     3061 tagaatagta tgaagtatgc ttacagaaag gttattagag atgacaaaag ctgaccagat
     3121 ttttaaagct aatattcaaa aaattataaa cgaaggatca ttgagtgagc aagcacgtcc
     3181 taaatataaa gatggtcgaa cagctcattc taagtatatt actggggcgt ttgctgaata
     3241 tgatttagct aaaggagagt ttcccatcac aactttacga cccattccaa ttaaatctgc
     3301 tataaaggaa ttattttgga tatatcaaga tcagtctaac agtttagatg tgctagaagc
     3361 taaatataat gttcattatt ggaatgaatg ggaagttgac cagacacgta ctattggaca
     3421 acgttatggt gctgtagtca aaaaacatga tatcatctca aaaatactaa agcaattagc
     3481 agaaaatcct tggaatcgtc gtaatgtcat ttctctctgg gactatgaag catttgagga
     3541 gacaaaaggg ttgttgccat gtgcctttca aatcatgttt gatgttagac gtgtagggga
     3601 agatctttat ttagatgcta gtttgacgca acgttctaat gacatattag ttgctcatca
     3661 catcaatgcc atgcaatacg ttgccttgca gatgatgttt gctaaacatt tcggatggaa
     3721 aataggtaag ttcttttatt ttgttaacaa tttacatatt tatgacaacc aatttgatca
     3781 ggctcaagaa ttactaaaac gacagccagt tgccagtcag cctaagcttg ttttaaatgt
     3841 tccagatggt actaatttct ttgatattaa accagatgat tttgaattac aaaattatga
     3901 tccagtgaag cctcagttgc actttgacct tgctatttaa actttattta gattttttgt
     3961 gagcattttg ttcataaatt tggtaaaata aatagagttt aaaatgaagg aaataattaa
     4021 tgacaaagga aattattgct atttgggcag aggatgaagc agggcttatt ggtgttgcgg
     4081 gtaagttacc atggtattta ccaaaagagt tagaacattt taaaaagaca acgctacatc
     4141 aagcaatctt aatgggccgt gtgacttttg aaggaatgaa ttgcaaaaga cttcctcaac
     4201 gtcaaaccct tgtcatgaca agaaatagag attatcaagt agatgaggtg ttgacaatga
     4261 ctagtattga aaaagtcttg gagtggtacc atgcgcagga taaaacactt tatatcattg
     4321 gtgggaataa ggttttagaa gcgtttaatg gttattttga tcgtatcata aaaacagtta
     4381 tccatcatcg ttttaaagga gatacctacc gacccaatct agattttagt catttcacgc
     4441 aagaaagcca aacgttctat gctcgagacg ctaaaaatcc ttatgatttt accgttactg
     4501 ctttaaaaca caaatagtct gaggagatga taagcgatgc aacgtagtat ttttggagta
     4561 tttacggctt ttttgtgcgt gatttgtgtt ctttgtgcca tcccagcttt taaaaagaat
     4621 cgttatggct taggaagtct gttcttatta aatgcgttta ctaatctggt taatagtatt
     4681 cacgccttct atggtacatt gttttgagat gcgacttgca aagacagcac tggtgaagat
     4741 ttagctatca agcaagaatc accaaaaacc aatacaacac attgactaat attaaaattc
     4801 agtaaaagat aagagaaagt aagagaatta taagaaatgg caggaagtag aactaacgat
     4861 attaaagtat actgttcatt ttgtggtaaa agccaggatg acgttaaaaa aattatcgca
     4921 ggaaataatg ttttcatctg caatgaatgc gtagctttat cacaagaaat tataaaagaa
     4981 gaattgtctg aagaagtcct tgccgatttg acagaagttc caaaaccaaa agagctcctt
     5041 gatgttttaa atcaatatgt ggttggtcaa gatcgtgcta aacgtgcttt atcagttgct
     5101 gtgtacaatc attataagcg tgtctcgttt acagaaagtc gtgatgacga tgatgtggat
     5161 ttacaaaagt ctaacatttt gatgattgga ccaacaggtt caggaaaaac cttcttagca
     5221 caaactctgg ctaaaagcct caatgtacca tttgctattg cagatgcaac ttctttgact
     5281 gaagcagggt atgttggaga agatgtggaa aatattttac tcaaactgat tcaggctgct
     5341 gactataatg tcgaacgagc agagcgtggt attatttatg ttgatgaaat agataagatt
     5401 gctaaaaaag gtgagaacgt atctatcaca cgtgatgtat ctggtgaagg tgtacagcaa
     5461 gcccttctta aaattattga aggtacggta gcaagtgttc ccccacaagg tgggcggaag
     5521 caccctaatc aagaaatgat ccaaattgat acaaaaaata tcttgtttat tgttggcggg
     5581 gcttttgatg gcatcgagga gattgttaaa caacgtttag gtgaaaaagt tattggtttt
     5641 ggtcaaaaca gccgtaaaat tgatgacaat gcttcttata tgcaagaaat tatttcagaa
     5701 gatattcaaa agtttgggct tattccagaa tttattggcc gcttaccagt tgtggcagcc
     5761 cttgaacaac ttaaaacatc agatttgatt cgaatcttaa cagagccaag gaatgctctt
     5821 gtcaagcaat atcaggccct tctttcctat gatggggttg aattagaatt tgataaagca
     5881 gctcttgaag ctatcgcaac taaagcgatt gaacgtaaga caggagcacg tggtcttcgt
     5941 tctattattg aagaaacaat gcttgacatt atgtttgaaa ttccaagcca agaagatgtc
     6001 acaaaagtgc gtatcaccaa ggctgctgtc gaaggcaagt caaaacccgt tttagagaca
     6061 gcttaaggag aaataatggc tgaagaacaa gtcttaaata cccataacgc ctcaatttta
     6121 ttgagtgctg ccaataaatc tcattacccc caagatgatt tgccagaaat tgccctcgct
     6181 ggacgttcaa atgttggtaa atcaagtttt attaatacca tattggggcg caaaaatttg
     6241 gcaagaacct caagcaagcc aggaaaaacc caactattaa attttttcaa tattgatgac
     6301 aagcttagat ttgtggatgt ccctggttat ggctatgcca aggtttctaa aagtgaacgg
     6361 gccaaatggg gaaagatgat cgaagaatac ttaaccacca gagataatct gcgtgctgtc
     6421 gtgagtttgg ttgatttacg tcatgcccct tcaaaagaag atattcagat gtatgacttt
     6481 ttgaagtact atgacattcc tgttattgtt gtggcaacca aagcagataa gattcctcgt
     6541 ggcaaatgga acaaacatga gtctgtggtc aagaaagctt tgaattttga caaatctgac
     6601 acttttatag tattttcttc ggtagaacgt attggtatcg atgattcttg ggatgctatt
     6661 ttagagcagg tctagcgata ggtgttgatt tatctatgct aaaacattct cagtagaggg
     6721 tgtttttctt atcgaaaaca taaaaaatca tagttaggtt taccgatttt acttgacgcc
     6781 ttaatgatga catgctagta ttttatatta aggagatgag tcatgacaca agtagcccaa
     6841 gggcgctcta aaggaaagtc attgttatgc catcaatgca atgccctttt tgtatttatt
     6901 atttttctta tcttgcttgc tgtacttttt ggtgttcgat atcgtaatag ctctattgaa
     6961 ggtatctggc gaacaacaag cattgatcag aagttaggcg atgattttgc taaacgtctt
     7021 actggtttgc atcagagccc tcttattgat gatagtttat taacgtcatc tcagatgata
     7081 ctaacagtta aaaataacaa cgttgacttg agttttagtg ttcaagttga acgagacatt
     7141 tttgtcaaac gtttagctgc ttatcaccaa aatgaattat taaagacatt aaaggaaaat
     7201 catttagttg ttggtgactt gagtagtaaa gaacgccaaa ttattgagaa tagtatgcca
     7261 gctagccatg aattagaaat gattttagat caagcttttg agaaattagc ttctcagatc
     7321 ggaggcaaat ataatcaaaa aacaggacat ttatctgctg tggttctcaa aggaaaagtt
     7381 aatcgtattt tgcacaccat tgatatcaaa gaagaagtag cagcgggaca tacttctttt
     7441 tcaaaaggat tgctcacacc taatggttat tttgattaca ccagatttgg aaagaaactt
     7501 gagttattag gcgatgaaaa aattattttt aaaaaagcgc taaaaaaaag tccgtcaagt
     7561 gtataacgta gtcacaagac ggattttttc atgttttgaa aattattcaa ttgaagaagt
     7621 ttattactga tctttttgcc taatcactag ttgtccttct tttaggattg cctgaagttt
     7681 tttgacttct ggatggtcaa gataataatc agttatacgg tcacgaattt cctgttcgat
     7741 aatacgtcgt aatggacgag ctcccatagc gtgattgtac cctaaatcta tcaaatgagc
     7801 tttagcgtca tcagcaatcg ttaaactaat accttttttt gcggtggttt gatttacttc
     7861 agctagcatt aaatcaacaa tttcttctaa attttctttt tgcagatgat taaatttaat
     7921 gatactatta aaacggttca aaaactctgg tctgaaataa ggtgcaatgc gatccataat
     7981 gttagaccca gaagtttccg tatcttgttg gccaaaaccg gcatttgatg tagcaataat
     8041 aacggtattt ttaaagttaa tagtatttcc ttgtccatct gtcaatcgtc catcatctaa
     8101 cacttgtaga agaagtgtaa tgatttgagg atcagctttt tcaatttcat ctagtaagac
     8161 aatagcataa gggttacgac gaacacgctc agttagggta ttattattat catcgtagcc
     8221 aacataacca gccgttgtcc caatcaattt tgacacagca gtgcgatcgt tatattcgga
     8281 catatcaagt cgaataatag catctttgga accaaataaa tcaagtgcta attgtttagc
     8341 taactctgtt ttaccaacac cagttggacc cacaaaaagg aaagaaccta ttggacggtt
     8401 accatcatca aaacctgctc gatttcgtcg aatagcacgt gctactgctg cgacagcctc
     8461 atcttgtccg atgacatggc tttttaagcg gctgctaatt cctttgagcc tttctaaatc
     8521 attagtcccc atgttagaga caggaatacc agttaagcgc tctacagact ctgcgatatc
     8581 attgactgta gcagtaacat tttggctttg gttatgttgc tcaatttgct tttgtaatgt
     8641 ttcaatccgt gtttttgctg ctaaagcttt ttcaaagtct tctttggcga cagctttttc
     8701 ttgactctct ttttgtttag ctatttcagt ttcgagtgtt tttaagtcag tgaccgggtg
     8761 ttgcgcagct aaatgagcag ctgtcatatc cagtaagtca atcgctttat ctggcaggct
     8821 acggtgtggg atgtattgaa tagaataatc aacagcagct tttagaacat tatcaggaag
     8881 ggtaatatgg tggtgttgtt cataaagatt acgaataccc attaaaatgt gaaaagtatc
     8941 ttcagctgat ggtgcattca ctttaacttc attaaagcga cgagcaagag cagcattttt
     9001 taaaatagta ttgcggtatt cgtcttgagt agtagcacca atcagtgtta attctcctcg
     9061 tgacaaggct ggtttaagaa tatctgcaag tccctttgag cctgaatcac tgcttgtggc
     9121 accagcacca actatttgat gaatttcatc aaaaaataag atgatattcc cagcttcttt
     9181 tacttcttga atcaagtttt gaatggtctc ttcaaaggaa ccacgatact gtgtaccagc
     9241 ttcaagacta gaaatatcaa ttgatacgat ttctttgttt ttaatagcgg ctgggacatc
     9301 tccattaaca atggcttgag ctaaaccttc aatgactgca gttttaccga caccagcatc
     9361 tccgacaagg acaggattat ttttagttcg tcgtgccaag atttcagcgg tatcttgaat
     9421 ttctttgtta cgaccaatga cggggtcaag atgcccttga cgagcttctt gtgttaagtt
     9481 agttccgagt tgagtcaata cactatcagc tttaggtgtt aacatttgag aatttgttgc
     9541 tttggttgtc gctacaggga gttgtcctgt ttgacgatag tgttggaatt cttcgggagt
     9601 tatttcgcga ccgttaacga gatagcgggg attttcgcta cgataaccac ccatgtttgc
     9661 cattaattgg ttgaaaatat catccatatt tacaaaagga tctttaccag aaaaatgtgt
     9721 catatcagtt acctcgcaat aaaaaataga ctataagatg tttgtaagca gtcgcagaat
     9781 cgttgttagg atttcacatg ataggaaacc ctttgctaat aaaatgattc agacattatt
     9841 tttactcatc ttatagtatt attataatca aaaggtcagt aaaggtcaaa caaaaacact
     9901 tgattttcaa aaagctaaat aaacacgaaa agtaagtgct tatatgatga ctttagaata
     9961 aatcataagt tttaaaaaga tcatcctatt ctatagcact taggctgtca atgagagaaa
    10021 aacaaatgtt ttcattaaca agttggcgct ttcatggtat aataaagtgc taagacgcaa
    10081 ttctattata agggggaatc atggaagcac tcaaaaaaat tgccggtgtt accgctgcgc
    10141 aatatgtgac tgatggcatg accattgggc ttggaactgg gtcaacggct tactattttg
    10201 ttgaggaaat tggccgtcgc gtaaagcaag aaggactgca agtggttggt gtgactacgt
    10261 caagtgtgac tagcaagcaa gcagaaggct taggcattcc tttaaaatcg atagatgata
    10321 ttgatagcat tgacctcacc gtagatggtg ctgatgaagt ggataaggat ttcaatggaa
    10381 tcaagggtgg cggcgcagcc ttactaatgg aaaaaattgt tgcaacacca acaaaagaat
    10441 acatctgggt tgttgatgct tctaaaatgg tagagcatct gggtgccttt aagttaccag
    10501 tcgaagtcgt tcaatatggt gctgatcgtt tgttccgtgt ctttgaaaaa gctggttaca
    10561 aaccgtcttt tcgtatgaaa ggtgatagtc gtttggtgac agacatgcaa aactatatta
    10621 ttgatcttga tttgggttgt attaaagatc ctgttgcttt tggtcacctg ctagatggaa
    10681 cagttggtgt tgtggaacac ggtctcttta atggtatggt tgataaggtg attgttgcta
    10741 gtaaagacgg cgttattgtt ttagaagcgc ctaaagcagg ctagtcttag catataacag
    10801 tgttaatata ttttataggt attggggagt caaccgtaac ctcccctaga ttggagactt
    10861 atgtcaaaat ttaatcgtat tcacttggtt gttttagact ctgtcggtat tggtgcagcg
    10921 ccagatgctg acaaattctt taatgctggt gtagctgata cagattctga caccctcggt
    10981 catatttcag aaacagcagg cttatcggtt ccaaatatgg caaaaattgg acttggaaac
    11041 atttctcgtc cgataccact aaaaactatt ccaacagaag acaatcctac tggttacgtg
    11101 accaaattgg aagaagtatc tcttggaaaa gataccatga caggccattg ggaaattatg
    11161 ggcttgaaca ttacggaacc ttttgacact ttctggaatg gtttcccaga agagatttta
    11221 actaagattg aagaattctc aggtcgcaaa attattcgtg aagctaataa accctattca
    11281 ggaactgcgg ttattgatga ttttggtccg cgtcaaatgg aaacaggtga gttgattgtt
    11341 tacacctcag cagacccagt gcttcaaatt gcagcccatg aagacattat tcctgtggaa
    11401 gagctttaca agatttgtga atatgctcgc tccatcacgc ttgaacgccc tgccctttta
    11461 ggccgtatta ttgcgcgtcc ttatgtaggc gaaccaggaa actttacacg tacggctaac
    11521 cgtcatgact atgcagtgtc tcctttccaa gataccgttt tgaacaaact ggctgacgca
    11581 ggcgttccaa cttatgccgt tggtaagatt aatgacatct ttaatggttc aggtattaca
    11641 aacgacatgg gacacaataa gtcgaacagc catggtattg acacgcttat taagactctt
    11701 caattacctg agtttactaa gggattctcc tttactaacc tcgttgattt tgatgccaac
    11761 tttggtcatc gtcgtgaccc agagggttat cgtgactgct tgcatgaatt tgacaatcgc
    11821 ctacctgaaa tcttcgcaaa tatgaaagaa gacgatttgc ttctcatcac tgctgaccat
    11881 ggtaatgacc caacctatgc aggcaccgac cacactcgtg aatacattcc attgcttgcc
    11941 tactctgcat cctttactgg aaatggtctt attccacaag ggcattttgc ggatatttca
    12001 gcaactgttg cagaaaattt tggagttgat acagccatga ttggggagtc attccttggt
    12061 cacttaaaat aagaaagtta gtaattgata atggaagaaa tcacgattta tcataatcca
    12121 aactgtggca cttcacgtaa tgttcttgcc atgattcgcc atgcaggtat tgagcctact
    12181 attattgagt atttgcagac gccacctaat cgggagactt taattgagct cttacaatcg
    12241 atgggaatct ctgcgcgaga actcttgcga accaatgttc ctgaatttga ggctcatgga
    12301 ttagcaaatc aagcagtagc tgagaaagac gttattaatg ccatgttggc ggatccaatt
    12361 ttgatcaatc gtcctattgt tgtgactcgc aaaggagtaa aactttgtcg tccttctgag
    12421 accttactgg atattcttcc tgttcccctg cctagtcctt atatgaaaga ggatggcgag
    12481 agtgtgaacc ctattgaggt aaaaggagaa aaagatgtca ttgatgacta aaattaacga
    12541 aacaaaagac tttttagtta ctaaaggaat tgaaactccc gaatttggat taattctggg
    12601 ttcaggcctt ggtgaacttg ctgaagaagt agaaaatgct attgttattg attatgcaga
    12661 tattccaaac tggggcaagt caacggttgt tggccatgct ggaaagttag tttacggaga
    12721 tttagctggc cgtaaagtgc tcgccttaca aggacgtttc catttttacg aaggaaatcc
    12781 gcttgaagtg gtgactttcc ctgttcgtgt catgaaagct cttggatgtg aaggcgttct
    12841 tgtcacaaat gcagctggtg gtatcggcta cggcccaggt accttgatgg caatcacaga
    12901 ccatatcaat atgaccggaa ataacccact aattggtgaa aacttggacg agtttggtcc
    12961 acgcttccct gatatgtctg atgcctatac taaggtttat cgcaataagg cacatgaaat
    13021 ggctgaaaaa atgaacatca aactagaaga tggtgtttat atgggcttga ctggtccaac
    13081 ttatgaaaca ccagcagaaa tccgtgcttt caaagtatta ggagcagatg ctgttggcat
    13141 gtcaactgta ccagaagtta ttgtggctgc tcattctgga cttaaagtac ttggtatttc
    13201 agctattaca aactttgcag ctggttttca atcagaattg aaccatgaag aagttgtgga
    13261 agtgacccaa catatcaaag aagatttcaa aggtcttgtt aaagctattt tagcggagtt
    13321 gtaggacgaa caaaatcaac tggagcatta ttacaggaat gttattttat gtcgtcagtt
    13381 tggcacatct gttcgcaata aaaaaatcaa aaaataatca cggagtgtga gttccatgaa
    13441 tgttgaactc agattctagc ttttctaact aacataacca acgaagggaa agagacgtat
    13501 tcggtttaaa ttgagtatgg actcgaacat ttcttcgtat taatttttaa ataataaagg
    13561 aaagagagtt agtcctttct tatttgaact cgatttttag ctctcttcta ttcaatatca
    13621 attatgaaag gaatggggtt gtattcaatt gaatacgggc ggaaagctcg gaaactagat
    13681 aaactaactt ttgagctcac acatggcgtt agttccctaa atttcagtcg ctttctgtcc
    13741 gccctctgac cttctacaaa ataaaaggaa aggtagggct aactgtattc attgaattga
    13801 atacgggcgg agaactgttt ttcttcctta taaaaaataa aaaaataaga aaggtgaggg
    13861 ctgagttcat taatttgaat tcgggctaag gacttggtca aaaagataag ttgtctagaa
    13921 aatcaggatt ttctgccaac ttcctatttt gccatcgttc ttttaacgcc ctcagtatca
    13981 taatttatgt ctatccatat ttctgccaaa aaaggcgata ttgctgataa aattcttcta
    14041 cctggagatc ctttgcgcgc gaaatttatc gctgaaaatt tcctagaaga tgcagtgtgt
    14101 tttaacgaag tgcgtaacat gtttggctac acaggaactt acaaagggca ccgcgtttct
    14161 gtcatgggaa ctggtatggg gatgccttcg atctctatct atgctcgtga attaatcgtt
    14221 gattacggtg tcaaaacatt gatccgtgtt ggtactgcgg gtgcgattga cccagaagtt
    14281 catgtgcgtg aattggtgct tgcccaagca gcagcaacca actcaaatat tatccgtaat
    14341 gacttcccag aatttgattt cccacaaatt gctgattttg gtttacttga caaggcttac
    14401 catatcgcca gagaaatggg agtaacaact cacgttggga atgtgctttc ttctgatgtc
    14461 ttttacacta acatgcctga gcgtaatatg gctcttggta aattgggtgt taaagccatc
    14521 gaaatggaag cagcagctct ttactattta gctgctcagc accatgtcaa agcccttgga
    14581 atcatgacga tctcagataa cttgaatgat ccaaccgaag atactacagc tgaagagcgc
    14641 caaaccactt tcactgatat gatgaaggtc ggcttggaaa ccttaattgc caatgactaa
    14701 taatcaaaca ctagacatcc ttttggatgt ctatgcttat aatcacgcct ttagaattgc
    14761 taaagccttg ccaaatatcc ctaaaactgc cctctattta ctagagatgt taaaagagcg
    14821 cagagaattg aaccttgcct ttctggcgga acatgcagca gagaatcgga tcattgaaga
    14881 ccagtaccac tgttcattat ggcttaacca atcgcttgaa gatgagcaga ttgccaatta
    14941 cattttggat ttagaagtta aagtaaaaaa cggtgctatt attgatttcg ttaggtcagt
    15001 gtcgcctatt ctttaccgac tttttctcag actaatcacg tcagaaattc caaacttcaa
    15061 ggcttatatt tttgatacaa agaatgacca atatgatacc tggcattttc aggccatgtt
    15121 ggaatctgat cacgaggttt tcaaggctta cctgtctcaa aagcagtctc gcaatgtgac
    15181 gaccaaaagc ttagcagacg tgttgacgtt gacctcctta cctcaggaaa tcaaggactt
    15241 ggtttttttg ttacgacatt ttgaaaaggc tgtccgtaat cctctggctc atttgattaa
    15301 gccttttgat gaagaggaac tgcatcgcac cactcatttt tcttctcagg cttttttgga
    15361 aaacattatc accttggcga ctttttctgg tgtaatctac cgatgtgagc ctttttactt
    15421 tgatgacatg aatgccatta ttaaaaagga gttgagcctt tggagacaat ctattgtctg
    15481 atatttatca aaaaacacga taacttgacc gatacaggtc ttactaccgt gtttttttag
    15541 tatgacgatt gaaaaagaaa ttaatcaaag gttacttctt cgagaaggta ttcgataaaa
    15601 cgttccccca ttttggacaa gttggctttt tcatgcttga taaaaacaat gtcaatttcg
    15661 tcagggacat ctagcgggat agccacaatc tggtcaccat ttagattgct atttagaata
    15721 ccactggcaa cggtgtagcc atctaaacca atcattaagt taaagagggt ggcacggtca
    15781 ctgaccacaa ttgatttgct gtggggcatc tgggacatca tctcttctga aaaataaaag
    15841 gagttgtgaa tcccttggtc atagctcagg taaggaaaat cgctcaagtc gtccatgctt
    15901 aataggcttt tagttgctag aggatttgat ttgctgacaa aaatatgagg ctgtgcctta
    15961 aataatggag aggcagtcaa atgattgtca tcaaataatt tggtcaagac atctcggtta
    16021 tagtcgttga taaagagaac accaatttca gatcgaaagt ttttgacatc atcaatgatt
    16081 tcccaagttc ttgtctcgcg caaaaagagt tcataccgag tcatgtcggt ccgcttgagg
    16141 agtgagacaa atgcattgac tacgaaggca taatgttgag atgaaacgct aaagagctct
    16201 cggccagtat tgtggttttt atagcggtct tctaacagtg atgtttgctc tataatttgt
    16261 cgagcataag ataaaaattc caccccatct tttgtcaagg tgatcccttt aggatttcgg
    16321 ttgaaaatgg taatgcccat ctccatttcc aaatccttga ccgcattgga tagacttggt
    16381 tgtgtgataa ataattgctt agcagcttca ttcatagagc cgcactcaac aattttgata
    16441 atataatgca gttgttgaat tctcataagt acagtctagc tcaaaaaaaa taaagacgca
    16501 agcacaaacg tcatcatggg tttagaactt tgaaaaagcg ctggagtctt ttctcaatat
    16561 ggcctaggat aaatatgatt caattttgag agttgaatct atcaatttca ttgccctttt
    16621 atttttcttg tgttatatta atagtattaa caaataggta tttttctaat caaagggtgg
    16681 tggtcgtgtg aatgttactg aacaaaatag tcatcaaatc ttaatccaaa agttattggt
    16741 cagtatccac tatttgactc tttttcgaga tgaactgaag cttgtagaac gaactccttc
    16801 tatacttgga ggggaatttc cagctcattt agtacagtct gagttgggag atattgttgc
    16861 ggccattgat accttggata tgcagcaacg cttgattgag tctacctttt ggtatgaaga
    16921 atcagctttt aagttgatga acaaaacttt ggatattgtc gataattgga ttaaaggagt
    16981 ggatcatctg atagatttat gccagtccaa agaagtcttt caaattatta ttggagataa
    17041 acgcattcga gtttttgggg tcttaagtga tgttttttca tctttaaaag tgagtgcttt
    17101 gtcacttaag gaagctccta ttccggatgt gctctatgaa catatccaga gggttaatct
    17161 tgaagaagaa acttttataa aatattacga aagccctaag gtatccacag gtgagtttga
    17221 ataaatgatt gggtaaaaat gataaaatga gttgacgtaa gtatacaaaa aaagatatag
    17281 taggaaaaat gtgttcaaat aacaaaaagt tttgacatct tgttttaaaa aagctataat
    17341 agtttaaatt tagacacctt taagtgagtc cagagaggca aacaaggtat acaagataaa
    17401 aaaagggaat tcccttttag tttgctgtag cctcgcttct ttggtagcga ggctttttgg
    17461 tgcttaatga gatattgaca cattatagaa aggataagtt atgaaagaag aacgcccaat
    17521 tattgcttta gacttttcat catttgagga gactaaagca tttttagact tatttccagc
    17581 agaagaaaag ctatacgtga aaattggtat ggaattatat tatgcgcaag gaccagatat
    17641 tgttcggtct atcaaatcgt tagggcataa tgtatttttg gatttaaaat tacatgatat
    17701 tccaaatact gttcgtgctg ctatggcagt tcttaaagaa ttagatattg atatggcaac
    17761 tgttcatgca gctggtggtg tagagatgct aaaagcagct cgagaaggac taggccaagg
    17821 ccctacctta attgcggtaa cgcagctaac ttcaaccagt gaagaccaaa tgcgtggtga
    17881 tcaaaatatt caaacgagtc ttttagagtc tgttttgcat tacagcaagg gggcagctaa
    17941 agctcagcta gatggggtgg tttgttcggc acaagaagta gaagctatca aggcggtgac
    18001 gcctacggga tttacttgct taactcctgg cattcgtcct aaaggcagta atattggcga
    18061 tcaaaaacga gtgatgactc caaaccaagc aagacgcatt ggaagtgatt atattgtggt
    18121 agggcgacca attacccagg caaaagaccc agtggctgct taccaagcca taaaagctga
    18181 gtgggcaggc taacagataa gattaggaaa ccaacacttg tctgttaaag aagaaaaagg
    18241 agaataagaa atgacactag caagccaaat tgcaactcaa ttacttgata ttaaagctgt
    18301 ttatttaaag cctgaagatc cttttacgtg ggcatctggc attaaatcac ctatttacac
    18361 tgataatcgt gtcacccttt cttatcctaa gactcgtgat ttaattgaga atgggtttgt
    18421 ggaaactatc agggctcatt tcccagaagt tgaggtcatc gctggtacag ctacagcagg
    18481 tattcctcac ggtgccatta tagcagataa aatgactctt ccttttgttt atattcgcag
    18541 taagccaaaa gatcatgggg caggcaacca gattgagggg cgtgtgctta aaggccaaaa
    18601 aatggttatc attgaagatt tgatttcaac aggtggttcg gtactagatg cagccgcagc
    18661 agccagtcgt gagggagctg acgttctggg agttgttgcg attttcactt atgagttacc
    18721 aaaagctagt cagaatttta aagaagctgg tatcaagttg attacgcttt ctaattatac
    18781 tgagctaatt gcagttgcaa agttgcaagg ctatattact aatgatggtt tacatttact
    18841 taaaaaattt aaagaagacc aggtaaactg gcaacaataa ggaactatca gccaggttta
    18901 tttttaatga aaaaatctct ttagccccaa ttcaaaatgt tatgttttga ttgttttgtc
    18961 tacattcctt acacatcaca ttctaatttc taacctttta atggtggttt tcttttattt
    19021 gtgttttaat aaaaaagaat gttttaggat tcatatctta gaaaggaaga gtagctatga
    19081 cttatcaaga tgcgacggct atggctattg ctgttcagac tggtcagacc acaccactag
    19141 aattagtaac ccaggctatt tataaggcta aaaagttgaa ccctacctta aatgctatta
    19201 cctcagaacg ctttgaagca gccctagaag aagccaaaca aagggatttt tcaggtctgc
    19261 cttttgctgg tgtgcctctt tttttaaaag atttgggaca agaattaaaa ggtcactcat
    19321 caacctcagg ttccagattg tttaaagagt atcaagcaac taaaacggat ttatttgtga
    19381 aaagattaga agctttaggc tttattattt tagggcgctc caacacgcct gaatttggct
    19441 ttaaaaatat tagcgatagc agtttgcacg gaccagtgaa tttaccaaag gataatacac
    19501 gaaatgctgg tggctcatct gggggagctg ctgctctagt gtcatcagga attagtgcgc
    19561 tagcaactgc tagtgatggt ggtggttcta ttcgaattcc agcctcgttt aatggcttaa
    19621 ttgggctcaa acctagccgt gggcgcatgc ctgtcggacc tggttcttat cgaagctggc
    19681 aaggagcctc agttcatttt gccttgacca aatccgttag agatactaga aatctactct
    19741 actatttaca gatggaacaa atggaaagcc ccttccccct agccacctta acaaaagata
    19801 gcatttatca atcgctacaa cgtcccttga caattgcttt ttatcaacgg ttaagtgacg
    19861 gtagtccagt atctttagat actgcaaaag ctttaaaaca agcagtaact tggctaagag
    19921 aacaagggca tcagctagtt gaattggaag agtttcctgt aaatatgaca gaagtgattc
    19981 gtcattatta tattatgaat agtgtggaaa cggctgctat gtttgctgat attgaagaca
    20041 cttttggacg tccgatgacc aaagacgata tggaaaccat gacctgggct atttaccaat
    20101 caggtaaaga catcccagct tggcgttact cacaggtcct tcaaaaatgg gatacgtaca
    20161 gtgcaaccat ggctagtttt catgagactt atgacctgtt gttgacgttc acaaccaaca
    20221 caccagctcc taagcacgga gaacttgtcc cagattcgaa actcatggct aacctggctc
    20281 aagctgaaat tttcagcagt gaagagcaat tcaatttggt cgaaaccatg tttggtaaga
    20341 gtctggctat taatccttac acagctctgc ctaatctaac tggccagcca gctattagtt
    20401 tacctactta cgaaactaag gaagggctcc caatgggcat tcaactaatt gcagccaaag
    20461 gtagagagga cctattatta ggaattgcag agcagtttga ggcatcagga ttattgaaga
    20521 ttcctcaata ataatcttat aaaacagttt aactattata gattaacttt ttaaagagat
    20581 tgagcgctca ctgataacac aacttaatca aaatgttata ctattcttat cttaataaag
    20641 gagttactat gaagaaatca gttactttac tgtctatcgg acttgccagc cttttattgg
    20701 cagcatgtgc gccacataaa tctcaaaaat caagttggga cactatcaaa gaaaaagggg
    20761 tgttaaaagt agctacccca ggaacctatc agccaacttc tttttacaac gataataatg
    20821 agttagtagg ttacgaagta gatatggtca aagaaatcgg taaacgactt aacattaaag
    20881 tcaagtttgt tgaaacagga tttgaccaag cgtttacctc agttgatagt ggtcgagtgg
    20941 atatttcttt gaacaacttt gacattaccc caaaacgtca gaaaaaatac aatatctcta
    21001 cgccttataa atacggggta ggaggcatga ttgtccgtgc tgatggcagc tcaaacatgg
    21061 ctaaaaaaga tcttagcgac tggaaaggaa aaaaagcagc tggtgcttct ggaacagaat
    21121 acatgaaagt agcgcaaaaa cagggagccg aactggtgac ctacgataat gttacaggag
    21181 atgtttatct aaacgatgtg gctaacggtc gaacagattt catcccaaat gattacccag
    21241 ctcaaaaatt atttgtggat tacatgttat ctcaaaatcc aaatctaaat gtcaaaatga
    21301 gcgatgttca gtacaaccca accgaacaag gaattgttat gaacaaaaaa gacgatagcc
    21361 tcaagaaaaa aatagatgct gttattaaag acatgataaa agatggtagc ttgaagaaaa
    21421 tatctgaaac ctactatgct ggtcaagatt tgacaaaacc ttttggcaaa gacaaaaaaa
    21481 ttcccgtcat tgatacgaaa gacgttaact aatacttgat gttaaaggag tcctaaccac
    21541 atgattaata tcccattaat gaaagatagt ttaggatttg tgttatcggg attaccttat
    21601 acccttggta tttcgttgct cagttttttt acgggtctgt ttttaggttt aggcctagcc
    21661 cttctgggaa ggtcacgtca gccgttgatt cattatcttg ttagagcgta catctctatt
    21721 atgcgaggtg tgccgatgat cgtggtgctc tttgttcttt atttcgggtt gccttattat
    21781 ggtttagaat tgccagcttt actttgcgct tatcttggtt tttctatggt tagcgctgcc
    21841 tatatttctg aagtttttcg ctcttctata gaagctattg ataagggaca atgggaagca
    21901 gctaaggctt tagggttgcc atatgctctt atggttaaga aaattattct tcctcaagcc
    21961 tttcgaattg cagttcctcc tctaggcaat gtcattattg atatggttaa aagttcgtcg
    22021 ctagctgcca tgattaccgt accagatatt tttcaaaatg ccaaaattat cggtggtagg
    22081 gagtgggact acatgtccat gtacatttta gtagccttta tctattggat tattgctttt
    22141 ttgttagaac gttaccaaga attcttagaa aataagctag cacttgttta atatcctgta
    22201 aaaaacgaat attaaccctg gaaatatcat attttcaggg tctttctatt taaaatgtgg
    22261 taaactagat agaaaaggac atcgtattta acttagaaac tgacgtgatt agaaaggaat
    22321 cttatggctc attcaatttg gcatgagaaa atcaaatcat ttttgccgga gcattactac
    22381 ggtcgcatca atcacttttt agatgaagcc tatgcttccg gtcttgtcta tcctccacga
    22441 gaaaatgtct ttaaagcctt acaagtcact cctttggaag aaaccaaagt gttgatttta
    22501 ggacaagacc cttatcatgg ccccaaacag gcccagggct tatcattttc agttccagaa
    22561 gagatttctg ctccgccatc ccttattaat attttaaaag aattagcaga tgacattggt
    22621 cctcgtgacc atcatgattt aagcacttgg gctagtcaag gggttctact tttgaatgct
    22681 tgcttaacag tgccagcagg ccaggctaat gggcatgcag gcttaatatg ggagccattt
    22741 acagacgctg ttattaaagt gctgaatgag aaagacagcc ctgtagtttt cattttgtgg
    22801 ggagcctatg caaggaagaa aaaagccttc attactaatc caaagcacca catcatcgag
    22861 agccctcatc caagcccttt gtcatcttat cgtggctttt ttggcagcaa acccttttca
    22921 agaaccaacg ctattttaga aaaagagggc atgactggcg tagattggct aaaataagcc
    22981 agccatttca actaaatagc ttaagctaga cattttgata cctttaataa gtgaaatgag
    23041 tgcagtagat ttttcctatt aaagaacgta aggtcttatg gctaaaacag ataaataaag
    23101 gagaaaacat gatactcatc aaaaatggac gcgttatgga cccgaaatcg cagagagatc
    23161 aagtggcaga tgtcttgatt gatggtaaac agattgtaaa gatagcttca gctattgaat
    23221 gccaagaagc tcaagtgatt gatgctagtg gtcttattgt tgctccgggt ttggtcgata
    23281 ttcatgtgca ttttagagaa cccggtcaaa cgcacaaaga agatattcac acgggagcct
    23341 tggcagcagc agcaggtggt gtgactacgg ttgtcatgat ggctaatacc aatcctgtca
    23401 tttcagatgt cgaaacttta caggaagtgc tagctagcgc agctaaagaa aagattcata
    23461 tttacaccaa tgccagtgtc acccaagcct ttaatggaaa agacgtgact gattttaaag
    23521 ctttgttgga agcaggagca gtaagctttt cagatgatgg tattccctta gaaagttcta
    23581 aagttttaaa agaagccttt gatttggcta atgccaacca aactttcatt tctttgcatg
    23641 aggaagatcc tcagttaaat ggggttcttg gttttaatga aggtattgct gaggagcatt
    23701 ttcatttttg tggggcaact ggtgtagcag aatatagtat gattgcaaga gatgtcatga
    23761 ttgcttatga caggcaagct catgttcata tccaacattt atctaaagca gaatcggttc
    23821 aagtagtggc ttttgctcag cagttgggtg ctaaggtaac tgctgaagtg tccccacaac
    23881 atttttcaac aactgaagat ctcttattga ccgcaggtac cagcgccaag atgaatcctc
    23941 cccttcgtac acaaagagat cgcttagctg tcatagaagg cttgaaatcg ggtgttatca
    24001 cagtcattgc cactgatcat gctcctcatc acaaggatga aaaagctgtt gatgatatga
    24061 ctaaagcccc atctggtatg acaggacttg aaacttcttt gtcattaggt ttgacgcatt
    24121 tggttgaacc aggtcatctt acacttatgt cactattaga aaaaatgacg cttaatccag
    24181 ctttgcttta tggttttgat gcgggttatt tggcagaaaa tggtccagca gatttggtta
    24241 tttttgcaga caagcaagaa cgtctgatca ctgagaattt tgcttctaaa gctagtaatt
    24301 ccccatttat tggtaacaaa ctcaaaggtg tcgtgaaata cactattgca gacggagagg
    24361 ttgtttaccc taattaacaa atgagttaaa acaaaaaagc ttgactttca gaggaagtca
    24421 ggcttttagt aaatttattt tttaggcact tgtttgctta aatttagccc ccaaggtatt
    24481 agattttcag tatgatgttt gatacgacta atgttatctt tgtgtcttat aataataatg
    24541 aacgcaagta atatcacaat aaaagttaaa aagtagtcat aatttggtaa aagaaaatga
    24601 atggcaggaa atgttaaaac agataacaca ccaacgatag ctgaaaccac actagctaaa
    24661 gatatcatgc taaataaata gagaactaaa acaaagatag atgctaaaaa aaagagatat
    24721 aacggagcaa agcctagcaa tacaccagca cttgttgcta cggccttacc acctttaaag
    24781 ttggcaaaaa taggaaaagt atgccctaaa actgcgaaaa agccgatagc aatggatgaa
    24841 attgaagtca taccaaaaat aattggtaac aatattgaaa gtgtcccttt aaacatatca
    24901 atagctaagg tagctgttcc tgccttgaca cctaaaatcc gaaaagtatt tgtggttcca
    24961 gtatttcctg atccatgctc tcgtaagttg atgtggtaaa agtactgtcc aatccatagt
    25021 ccagttggaa tagaaccaag taaataggca atggtaataa aaagtagtaa tttcataatc
    25081 cttattataa catattcata gattggagaa ctagatctgg aaaagaattc ggaaaaaatt
    25141 agcaaattat tttgcaaaat tggtaaaaaa cttgtaagat aaaaaagatg aaactgtctg
    25201 gaggtcggtg ttgactaaga aagaaattac aattaataat tataatgacg atgctatcca
    25261 agtcctagaa gggttagatg ctgttcgcaa gcgtccaggg atgtatatcg gttcaacaga
    25321 tgctacggga ttgcaccacc tgatttggga aattgttgat aatgctgtcg atgaggcctt
    25381 gtctggtttt ggagatgata ttaaggttgt tatcaataaa gatggctcgg tcagtgtggc
    25441 agatagcgga cgtggaatgc caacaggcca gcatgccatg gggattccca ccgttcaagt
    25501 tatttttacg attcttcatg caggtggtaa atttggtcag ggtggctaca aaacgtcagg
    25561 tggtttgcac ggtgttggct cctctgttgt caatgctttg tctgcctggt tagaagtcga
    25621 aatcacaaga gacgggtcag tgtatcgtca gcgctttgaa aatgggggga aacctgttac
    25681 aacccttaaa aaagttggca cagctcctaa gtctaaatca ggaacggttg taacctttat
    25741 gccagatgat aagatttttt caactattga ctttaagttc aataccattt cagaacgatt
    25801 gaaagaatct gcttttcttt tgaaaaatgt caagatgagc ttgacagatc ttcgtggaga
    25861 tgacccgatt attgaagagt ttcattatga aaatggggtt caagatttcg tcgaatacct
    25921 caatgaagat aaggaaacct tgacacctgt tatttacatg gaaggtcaag accaagactt
    25981 tcaagtcgag gttgctttgc agtataacga tggtttctcc gacaatatct tatcctttgt
    26041 aaacaatgtg cgcaccaaag atggtggaag ccatgaaacg ggactaaagt cagctattac
    26101 caaagcgatg aatgactatg ctcgtaaaac caatctctta aaagaaaagg ataaaaatct
    26161 agagggctca gattatcgag aaggactctc agcagtatta tctatcttag ttcctgagca
    26221 gcacttgcaa tttgaaggac agaccaaaga caaattggga agtcccttag cgagacctat
    26281 tgtagagagc attgtttctg aaaaattaac cttcttttta ctcgaaaatg gagaagtagc
    26341 ttctcatcta gttcgcaaag ccattaaagc tagagatgct cgcgaagctg ctcgaaaagc
    26401 gcgtgatgac tcacgtaatg gtaagaaaaa caaaaaagac aaaggcttgt tgtcgggaaa
    26461 attaacccca gcccagtcca aaaatgccaa gaaaaatgaa ttgtatctgg tcgaaggaga
    26521 ttctgcggga ggttcagcta aacaaggacg agaccgcaaa tttcaggcta ttttaccatt
    26581 acgtggcaaa gttttaaata ccgaaaaagc taagatggct gacatcttga aaaatgaaga
    26641 aattaacact atggtttata ccattggagc tggagtggga gctgacttta atctcgagga
    26701 tatcaactat gataagatta ttatcatgac cgatgccgat accgatggtg cccacattca
    26761 aaccctctta ttgaccttct tttaccgtta tatgcgacca ttagtagaaa caggtcacgt
    26821 ttatattgcg cttcccccgc tttataaaat gtcaaaaggt aaaggtaaaa cagagaaagt
    26881 gtcctacgct tggacagatg gtgaattaga agatttacgt aaggaatttg gtaaaggagc
    26941 cattcttcaa cgctataaag ggctaggtga aatgaacgcc aaccaattgt gggaaaccac
    27001 catggatcct gaaaccagaa cccttattcg tgttactatt gatgatttag caagagcaga
    27061 acgacgcgtt tctgttttga tgggtgacaa agccgctcca cgccgtcagt ggatcgagga
    27121 caacgtcaag tttaccttag aagaaaatac ggtgttttag tcatacaagt ttctcacaga
    27181 taattacaag tgctaaagac gattgttttt taggtaatta ataaataatg aaaaagaaag
    27241 ctatttgaat atgtcaaaca ttcaaaacat gtcccttgag gatattatgg gagaacgctt
    27301 cggccgttat tccaagtata ttattcagga acgggcactt cctgatattc gagatggatt
    27361 gaaacccgtt cagcgccgca ttctttattc gatgaataag gatggtaata cttttgaaaa
    27421 aggttaccgt aaatcagcca aatcagttgg taatatcatg gggaatttcc acccgcatgg
    27481 ggattcctct atttatgatg ccatggtacg gatgagtcaa gattggaaga accgtgaaat
    27541 tcttgtggaa atgcacggaa ataatggttc aatggatggc gatccgcctg cggctatgcg
    27601 ttatacagaa gttcgcttgt ctgaaattgc gggttatctt cttcaagaca ttgagaaaaa
    27661 tacggtctct tttgcgtgga actttgatga cacagaaaaa gaacccaccg ttttaccagc
    27721 agcttttcct aacttacttg tcaatgggtc ttctggtatt tccgcaggtt atgcgacaga
    27781 tattccaccg cacaatttgt cagaagtgat tgatgccgtt gtttacatga ttgaccatcc
    27841 caaggctagc ttagagaaat taatggagtt tttgcctggg ccagatttcc cgacaggagg
    27901 cattatccaa ggagctgacg aaatcaaaaa agcctacgaa acaggtaagg gacgtgtcgt
    27961 tgttcgctct aggacggaaa ttgaggaact taaaggtggt aaacaacaaa ttattgtcac
    28021 tgagattcct tatgaagtta acaaagcagt cttggtcaaa aagattgacg atgttcgtgt
    28081 taacaataaa gtcccaggca tcgtagaagt acgtgatgaa tctgatcgca caggtcttcg
    28141 catagctatt gagttgaaaa aagatgcaga tagccaaacc attctaaatt atttgttgaa
    28201 atacacagac cttcaagtta attataattt caatatggtt gctattgatc actttacacc
    28261 acgccaagta ggtcttcaaa aaatcttatc aagctatatc agccaccgca aagatattat
    28321 tattgagcgg tctaaattcg ataaagccaa agctgaaaaa cgcctccaca ttgttgaagg
    28381 attgattcgt gtcttgtcca tcttagatga gattattgct ttgattagat cgtctgacaa
    28441 taaggctgat gctaaagaaa atctgaaagt cagctatgat ttctctgagg aacaagcaga
    28501 agcgattgta accttgcagc tttatcgttt aactaatacc gatattgtta ctctccaaaa
    28561 tgaagaaaat gatttaagag acttaatcac gaccctttcc gctattatcg gtgacgaagc
    28621 aaccatgtat aacgtcatga aacgtgaatt aagagaggtg aaaaagaaat tcgccaatcc
    28681 tcgtttatca gagttacaag cggaaagtca aatcattgag attgacactg ccagcttgat
    28741 tgcagaggaa gaaacttttg ttagtgtgac acggggcgga tacctcaaaa gaaccagccc
    28801 acgatccttc aatgcgtcta gtttagaaga agttggcaag agagatgatg atgaactcat
    28861 cttcgttaaa caggccaaaa caacagaaca ccttttgttg tttacaactt taggcaatgt
    28921 catttaccga ccaatccatg aattgaccga tttacgttgg aaggatatcg gtgaacattt
    28981 atcacaaacc atttcaaact tcgcgacaga agaagagatt ctctacgctg atattgtgac
    29041 ttcctttgac cagggcttgt atgtggctgt cacacaaaat ggttttatca aacgctttga
    29101 tcgtaaagaa ctaagtcctt ggcgaactta taaatctaaa tcaaccaaat atgtgaagct
    29161 aaaggatgac aaagaccgcg tggttacctt gtcaccagtt attatggaag acctcctatt
    29221 agtgactaaa aatggttatg ctttgcgatt ctccagtcag gaagtgccta ttcaagggtt
    29281 gaaatctgct ggtgttaaag gcattaactt gaaaaatgat gacagccttg cctcagcctt
    29341 tgctgtgaca agtaactctt tctttgtctt aactcagagg ggttctctca aacggatggc
    29401 tgtcgatgat attcctcaga caagtcgtgc caatcgtgga ctattggtcc tacgagagtt
    29461 aaaaaccaaa cctcatagag tttttctggc cggaggcgtt caatcagata cttcagctga
    29521 gcagtttgat ttgttcactg atatacctga agaagaaact aatcaacaaa tgcttgaagt
    29581 cattagtaag actggtcaaa cctacgagat tgctttagaa acgctttcgt tatcggaaag
    29641 aaccagcaat ggctccttta tttcagacac tatttcggat caagaagtac tggtagctag
    29701 aacacgttaa aaagagtgaa aactcttttt aattttatgg taaatttaga atattaatgc
    29761 aaaagaatag aataaacttg aaaattattg aaaaaaagtt acaatagaaa aaagcagaaa
    29821 agaggtacga tgatgacaat agcaattgat tgggacaacc taggatttga atatcataag
    29881 ttgccatttc gttacatttc ttattacaaa aatggtcaat gggataaagg tcaattaaca
    29941 gaagatgcca ccttacatat ctcagaaagc gctccagcgc ttcattatgg acaacaagct
    30001 tttgaaggtt taaaggctta tcgcaccaaa gatggctcta ttcaactatt tagaccagac
    30061 cgtaatgcag cgcgtttaca agccacagca gaccgcttgt tgatgccaca agtatcaaca
    30121 gagcaattta ttgatgcagc taagcaagtg gttaaagcca atgaagactt tgtaccacct
    30181 tacggaacag gtgcaacgct ttacctgaga ccgttattaa ttggagttgg agatattatt
    30241 ggggttaaac cagccgaaga atatattttc actatttttg ccatgccagt aggaaactat
    30301 ttcaaaggtg gacttgctcc aactaatttt attgtttctg aagcgtttga tcgcgccgct
    30361 ccttacggaa caggtgctgc taaggtggga ggaaactatg caggaagttt gctaccaggt
    30421 aaggccgcta aatcagcagg cttttctgat gtgatttact tagatccagc aacccatact
    30481 aaaattgaag aagtcggagc tgcaaacttt tttggcatta ctgcaaataa tgaatttgtt
    30541 acaccactta gtccatccat cttgccatct attacgaagt attcactctt acaactagct
    30601 gaagaaagac ttggtatgac agtaattgag ggtgatgttc caattaatga attggataaa
    30661 tttgtggaag caggagcttg tggaacagca gctgttatct ctccaattgg cggtatccag
    30721 tacaaggata acttacatgt cttttacagt gaaacagaag ttggtcctgt gacacgccgt
    30781 ttatacgatg agttggtcgg cattcaattt ggggatattg aagcaccaga aggttggatt
    30841 gtaaaagtag attaaaatag agcttgctct tgcaagcttt tttgattttg tgtaaaatag
    30901 acaaagtgag gtaaagtgat gagtaaaaaa gataaaaaaa ttgaaatcca actgattgat
    30961 cacaaagtta tggttaatga gactaagatt gatggctatc aattacaaat tggtaaacgt
    31021 gtggttggag aaattgcaga acttgatgag aaatttgctg tcttaaaaaa tgatggagta
    31081 gattgcttct tcaaaacgct cgaacaagca gttgaaaata tcattgaaaa ctacaatttg
    31141 aaccattaaa attagacaaa aacacttgcc agcttacaaa aactatgata gaatagtttt
    31201 tgttgttacg gaaagatagc gaagaggcta aacgcggcgg actgtaaatc c
//
LOCUS       DAUDYX010000019        29064 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.019, whole genome shotgun sequence.
ACCESSION   DAUDYX010000019 DAUDYX010000000
VERSION     DAUDYX010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29064
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.019"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..294
                     /gene="gpsB"
                     /locus_tag="VHD02_000850"
     CDS             <1..294
                     /gene="gpsB"
                     /locus_tag="VHD02_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEQ5497472.1"
                     /translation="FEQEFKTSMRGFDKKEVDEFLDNVIKDYENFNAQIEALKAENEA
                     LKKAKFQARNTVSATVQQPVPQPTRVAQSATNFDILKRISKLEKEVFGKQIIE"
     gene            308..676
                     /gene="rnpB"
                     /locus_tag="VHD02_000851"
     ncRNA           308..676
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VHD02_000851"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            739..1893
                     /locus_tag="VHD02_000852"
     CDS             739..1893
                     /locus_tag="VHD02_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEQ5497473.1"
                     /translation="MKETFRLVATAAAGLEAVVGKEVRALGFDCQVENGKVYFEGDVE
                     AIVKTNLWLRAADRIKIIVGQFPARTFEELFQGVFALDWGNYLPLGAKFPISKAKCVK
                     SKLHNEPSVQAITKKAVVKKLQKHFHRPEGVPLQEVGSTFNIEVSILKDQATIMIDTT
                     GSSLFKRGYRVQKGGAPIKENMAAAILALSNWFPDKPLVDPTCGSGTFCIEAALIGMN
                     IAPGFNRSFAFEEWSWVDKDMVQQVRDDAEQEANYEIELDISGFDIDGRMIEIAKSNA
                     EEAGLSDVITFKQMRLQDFRTDKVNGVVISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDELFELKYGQKADKKRKLYNGTLKVDLYQFYGERVKRHLI"
     gene            1907..3376
                     /locus_tag="VHD02_000853"
     CDS             1907..3376
                     /locus_tag="VHD02_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497474.1"
                     /translation="MSEESKEVEVTKESQTLGLNEAKSMTIGEAVRKQSEIKAGVTKD
                     DSILDKYIKQHRDEVSSQKFDAKYTELDTASLDNFIKKQREALSKAGLVDDEPVSAES
                     AEQDSTLVEEVAEDLAPMETTAVVTGIPVEATVPVLDLDPSERVIPEPQMTKEEPKRD
                     QFLSEDSHHPAKQNTKKGWLIALFLLLLAILAVVFGWNHFLRQDSGKTTQTASKQTKT
                     SLQTDSAKKATRLKAAAKAFEKQYGTFYTDATKSKLKNSAFATLPDLEAALKALEGSA
                     YYDKAKAKVDSLKKAIAAITAVNGKFVSDVVVDGEKVSAEVKADANFDDLSSATLTTG
                     NANLDAVLQASITEGRQQLASKAEAAKAANEQAAQAQAAQGQSTSVAPSGYGLTSYDP
                     ASLQRHLSRVPYNQDVIADRANPSWAFNPGVLEKIVATSQARGYISGYQYILEPVNII
                     NGNGYYNMFKPDGTYLFSINCKTGYFVGNGKGYADALDY"
     gene            complement(3522..4004)
                     /locus_tag="VHD02_000854"
     CDS             complement(3522..4004)
                     /locus_tag="VHD02_000854"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEQ5497475.1"
                     /translation="MTKEVIVESFELDHTIVKAPYVRLISEEFGPKGDRITNFDVRLV
                     QPNQNSIETAGLHTIEHLLAKLIRQRIDGMIDCSPFGCRTGFHLIMWGKHSSTDIAKV
                     IKSSLEEIATGITWEDVPGTTLESCGNYKDHSLFAAKEWAQLIIDQGISDDPFSRHVI
                     "
     gene            complement(4078..5409)
                     /locus_tag="VHD02_000855"
                     /pseudo
     CDS             complement(4078..5409)
                     /locus_tag="VHD02_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285054.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
     gene            complement(5480..6238)
                     /locus_tag="VHD02_000856"
     CDS             complement(5480..6238)
                     /locus_tag="VHD02_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEQ5497476.1"
                     /translation="MHLSLVLQAELENNRFTFLKEGKTVVFSDINQEKLDQVVADYTK
                     EGYDAFSVVCDVTKEVAINAAIDTVVEKYGRIDILVNNAGLQHVAMIEDFPTEKFEFM
                     IKIMLTAPFIAIKRAFPTMKAQKHGRIINMASINGVIGFAGKSAYNSAKHGLIGLTKV
                     TALEAADSGITVNAICPGYVDTPLVRGQFEDLSKTRGIPLENVLEEVLYPLVPQKRLI
                     DVQEIADYVSFLASDKAKGVTGQACILDGGYTAQ"
     gene            complement(6290..6949)
                     /locus_tag="VHD02_000857"
     CDS             complement(6290..6949)
                     /locus_tag="VHD02_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEQ5497477.1"
                     /translation="MGTSATVLSKEEIQTRIAKRVAQELEHNTLVNLGIGLPTKVANY
                     IPEGVTITLQSENGFVGLTGLTDDHYDPSIVNAGGQPVSIAPGGAFFDSSTSFGIIRG
                     GHVAATVLGALQVDKEASIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKAK
                     ILDKCTLPLTAQNVVNLIITEMGVFEYQDEGLCALEINPDYTFEDVQNVTEVTLIDKT
                     N"
     gene            complement(6951..7601)
                     /locus_tag="VHD02_000858"
     CDS             complement(6951..7601)
                     /locus_tag="VHD02_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEQ5497478.1"
                     /translation="MCKEITIKEAVAHVKDGDTIMVGGFMTNGTPEKLIDALVEKGVK
                     DLTLICNDAGFPDKGVGKMVANKQFSTIIASHIGLNREAGRQMTEGETVIDLVPQGTL
                     AERIRSGGFGLGGFLTPTGIGTEVAKGKEVITIDGKDYLLEKPLKADIALIFANKADK
                     NGNLQYAGSENNFNHVMAANAKTTIVEAREVVDVGQMDPNFVHTPGIFVNYLVKGA"
     gene            complement(7625..8812)
                     /locus_tag="VHD02_000859"
     CDS             complement(7625..8812)
                     /locus_tag="VHD02_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEQ5497479.1"
                     /translation="MKNIVIVEALRTPIGAFGGSFKSVSAVELGTTVLKKILDKTQVK
                     PEMVDEVILGNVLHAGLGQNVARQVAVHSGIPEDKTAFTLDMVCGSGLKAIQLAAQSI
                     MLGDADIVIAGGVENMSQAAYVSTQHRFGQRLGNSQLIDTLVHDGLTDAFNNYHMGIT
                     AENVAQKYGISREEQDQFALESQEKAARALENHRFADEIVPVSVPQRRKDPLIVTTDE
                     YPKVDTSLEKLQQLRPAFLPKEGTVTAGNASGINDGAALLMLMTEEKALELGLTPLVT
                     IESYASAGVAPELMGTGPIPATQKALKKAGLTISDLDLVESNEAFAAQSLAVLKDLKL
                     NPEIVNVNGGAIALGHPIGASGARILVTLIHEMKKRQVTRGLATLCIGGGQGTAVIVK
                     NNT"
     gene            9013..9908
                     /locus_tag="VHD02_000860"
                     /pseudo
     CDS             9013..9908
                     /locus_tag="VHD02_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988952.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10038..11645
                     /locus_tag="VHD02_000861"
     CDS             10038..11645
                     /locus_tag="VHD02_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEQ5497480.1"
                     /translation="MVNIILLIVSALIGLILGYALISIRLKSAKEAAELTLLNAEQEA
                     VDIRGKAEVDAEHIKKTAKRESKANRKELLLEAKEEARKYREEIEQEFKSERQELKQL
                     ETRLAERSLTLDRKDENLSSKEKVLDSKEQSLTDKSKHIDERQLQVEKLEEEKKAELE
                     KVAAMTIAEAREVILMETENKLTHEIATRIRDAERDIKDRTVKTAKDLLAQAMQRLAG
                     EYVTEQTITSVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVILSGFDPI
                     RREIARMTLESLIADGRIHPARIEELVEKNRLEMDNRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSFGQNVLRHSVEVGKLAGILAGELGENVALARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPVVVNTIASHHGDVEPDSVIAVLVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIATSFDGVQNSFALQAGREIRIMVQPEKISDDQVVILSHKVRE
                     KIENNLDYPGNIKVTVIREMRAVDYAK"
     gene            11755..12390
                     /gene="gmk"
                     /locus_tag="VHD02_000862"
     CDS             11755..12390
                     /gene="gmk"
                     /locus_tag="VHD02_000862"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEQ5497481.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFEYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEELIKTGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFVFLTPPDLDELEDRLVGRGTDSQEVIAQRIERAKEEIALM
                     REYDYAVVNDEVALAAERVKRIIETEHFRVERVIGRYDKMIKITKNPFKAK"
     gene            12406..12723
                     /locus_tag="VHD02_000863"
     CDS             12406..12723
                     /locus_tag="VHD02_000863"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEQ5497482.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGATPTQEFKSV
                     KSTLQALEEIESGNVVIHPDPSAKREAVRAKIEAERLAKEEEERKIKEQIAKEKEEEG
                     EKI"
     gene            12788..15172
                     /locus_tag="VHD02_000864"
     CDS             12788..15172
                     /locus_tag="VHD02_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEQ5497483.1"
                     /translation="MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGK
                     GNRLLQGFVIGFGQEDSSSLKLIQTVLDPEPVLNQEQLTLADQLRKTVFSYKITLLKA
                     MIPNLLNSNYDKVLRPESGLKKPDRDFLFEGKPSVLYSTLDREKEKIALKAIQAGHIT
                     VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQA
                     FSRDVVAYFVTNHLIRIDERQIDRSEPYFDQIKPSSFLTLNEQQASAVTEIVEQIGKP
                     SKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQMTSRFISRFGKQVA
                     IMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESN
                     PRYHAREVALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTQRANPLAKIPEV
                     TIVDFRDYIGQQAVSNFTPYLIDKIKERLVKKEQVVLMLNRRGYSSFVMCRDCGYVDK
                     CPNCDISLTLHMDTKTMNCHYCGFQKPIPITCPECHSNSIRYYGTGTQKAFDELQGVI
                     PEARILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNAD
                     TSLNLPDFRASEKTFQLLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKKQDFEAFY
                     RYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQVTELLKSHLSDNIKILGPTPK
                     PIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL"
     gene            15234..16169
                     /locus_tag="VHD02_000865"
     CDS             15234..16169
                     /locus_tag="VHD02_000865"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEQ5497484.1"
                     /translation="MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKD
                     IKVTPVKQLALEHGISIYQPEKLSGSQELIEIMGLGADGIITAAFGQFLPTLLLDSVS
                     FAINVHASLLPKYRGGAPIHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETD
                     NVGTLFEKLAIIGRDLLLDSLPAYLSGELKPIPQDHSQATFSPNISPEQEKLDWTMSN
                     QEVFNHIRGMNPWPVAHTFLEGQRLKIYEAQLAEGEGLPGQVIVKTKKSLVIATGQGA
                     LSLIVVQPAGKPKMSIIDFLNGIGRKLEVGDIIGR"
     gene            16159..17481
                     /gene="rsmB"
                     /locus_tag="VHD02_000866"
     CDS             16159..17481
                     /gene="rsmB"
                     /locus_tag="VHD02_000866"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEQ5497485.1"
                     /translation="MADNWKKSTRGKALLVIEAIFDQGAYTNIALNQQLSNKALSAKD
                     RALLTEIVYGTVSRKISLEWYLAHYVKDRDKLDKWVYYLLMLSLYQLTYLDKLPAHAI
                     VNDAVGIAKNRGNKKGAEKFVNAILRQFTSHPLPDMETIKRRNKYYSVKYSLPVWLVK
                     KLEDQFGSHRAVAIMESLFVRSKASIRVTDPLKLEEVAEALDAERSLLSATGLTKASG
                     HFAASDYFTNGDITIQDESSQLVAPTLNIDGDDIILDACSAPGGKTSHIASYLKTGKV
                     IALDLYDHKLELVKENANRLGVADNIETRKLDAREVHRHFEKDSFDKILVDAPCSGIG
                     LIRRKPDIKYNKESQDFNALQAIQLEILSSVCQTLRKGGIITYSTCTIFDEENRQVIE
                     AFLQSHPNFEQVKLNHTQADIVKDGYLIITPEQYQTDGFFIGQVRRVL"
     gene            17519..18259
                     /locus_tag="VHD02_000867"
     CDS             17519..18259
                     /locus_tag="VHD02_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEQ5497486.1"
                     /translation="MKISLKTDIGQKRSNNQDFINKFDNKKGITLVILADGMGGHRAG
                     NIASEMTVTDLGREWVKTDFTELSQIRDWLFETIQSENQRIYDLGQSEDFKGMGTTVE
                     AVALVESSAIYAHIGDSRIGLVHDGHYTLLTSDHSLVNELVKAGQITEEEAASHPQRN
                     IITQSIGQASPVEPDLGVRVLEPGDYLVINSDGLTNMISNDEIVTILGSKVSLDEKNQ
                     EMIDLANLRGGLDNITIALVHNESEDVE"
     gene            18256..20154
                     /gene="pknB"
                     /locus_tag="VHD02_000868"
     CDS             18256..20154
                     /gene="pknB"
                     /locus_tag="VHD02_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEQ5497487.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLANDLILDNEDVAIKVL
                     RTNYQTDQVAVARFQREARAMAELNHPNIVAIRDIGEEDGQQFLVMEYVDGADLKRYI
                     QNHAPLSNNEVVRIMEEVLSAMTLAHQKGIVHRDLKPQNILLTKEGVVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSIIEENHNVPQALENVVIRATAKKLSDRYGSTFEMSRDLMTALSYN
                     RSRERKIIFEDVESTKPLPKVASGPTASVKLSPPTPTVLTQESRLDQTNQTDALQPPT
                     KKKKSGRFLGTLFKILFSFFIVGVALFTYLILTKPTSVKVPNVAGTSLKVAKQELYDV
                     GLKVGKIRQIESDTVAEGNVVRTDPKAGTAKRQGSSITLYVSIGNKGFDMENYKGLDY
                     QEAMNSLIETYGVPKSKIKIERIVTNEYPENTVISQSPSAGDKFNPNGKSKITLSVAV
                     SDTITMPMVTEYSYADAVNTLTALGIDASRIKAYVPSSSSATGFVPIHSPSSKAIVSG
                     QSPYYGTSLSLSDKGEISLYLYPEVTHSSSSSSSSTSSSNSSSTNDSTAPGSNTELSP
                     SETTAQTP"
     gene            20277..20969
                     /locus_tag="VHD02_000869"
     CDS             20277..20969
                     /locus_tag="VHD02_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEQ5497488.1"
                     /translation="MKKFQFFLLIECILLAMGIMTILDNDLSSFILILVLILLALRFY
                     NQDSRNNFLLTVSLLFLFLIFMLNPYIIMAVLLGIVYIFINHFSQVKKKNRFALIRFK
                     EEKIEVNNTKHQWIGTANYESDYYCFDDINIIRISGNDTVDLTNVIVTGMDNIIVIRK
                     IFGNTTILVPIDITVTLDVSSIYGSVDFFRCQQYDLRNESIKLKETDNQSLKKVKIIV
                     TTIAGNVEVDRR"
     gene            20966..21970
                     /locus_tag="VHD02_000870"
     CDS             20966..21970
                     /locus_tag="VHD02_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ5497489.1"
                     /translation="MKKRYYALVWLYSTITILSIVFVVMDNLGITFNYLRNHLWQVER
                     LGFSILLLIVSVTLLLLLLWIIMDDNSKRNINQNLKYILNNRRLYLDETSEINTNLSR
                     LSKKMSHLTANMQKKESAYILDSQEVVKQERKRIARDLHDTVSQELFASSLILSGISM
                     SLEQLDKTQLQTQLTTVEAMLQNAQNDLRILLLHLRPTELANRTLSEGLNMILKELTD
                     KSDIEVIYKETIAQLPKTMEDNLFRIAQEFISNTLKHAKASRIEVYLNQTSTELQLKM
                     IDDGVGFDMDQVRDLSYGLKNIEDRVNDLAGNLHLISQKGKGVSMDIRLPIVKGDDDE
                     "
     gene            21963..22604
                     /locus_tag="VHD02_000871"
     CDS             21963..22604
                     /locus_tag="VHD02_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ5497490.1"
                     /translation="MSKIKVILVDDHEMVRMGLKSFLNLQADIDVVGEASNGREGVDL
                     ALALKPDVLVMDLVMPELGGVEATLEVLKKWKEAKVLVLTSYLDNEKIYPVIDAGAKG
                     YMLKTSSAAEILNAIRKVSKGELAIETEVDKKIKAHDQHPDLHEELTAREYDILHLLA
                     KGYDNQTIADELFISLKTVKTHVSNILAKLEVDDRTQAVVYAFRHHLVPQDDN"
     gene            22641..24041
                     /locus_tag="VHD02_000872"
     CDS             22641..24041
                     /locus_tag="VHD02_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ5497491.1"
                     /translation="MDAKLKYKAKKIKMVFFDIDDTLRVKDTGYMPESIQRVFKALKA
                     KGILVGIASGRARYGVPQEVQDLHADYCVKLNGAYVKDDAKTIIFQAPIPADVVVAYK
                     KWADDMGIFYGMAGRHEAVLSARNDMISNAIDNVYAQLEVCPDYNEYHDVYQMWTFED
                     KGDGLQLPAELAEHLRLVRWHDNSSDVVLKGTSKALGVSKVVDHLGLKPENILVFGDE
                     LNDLELFDYAGISIAMGVSHPRLQEKADVITKKVEEDGILYALEELGLIDKELQFPQL
                     DLENHTGPKVTIKTNHGDMTLVLFPEHSPKTVANFLGLAKEGYYDGIIFHRVIPEFMI
                     QGGDPTGTGMGGQSIYGESFEDEFSDELYNLRGALSMANAGPNTNGSQFFIVQNSKIP
                     YAKKELERGGWPTPIAAAYAENGGTPHLDRRHTVFGQLADDASFKVLDLIASVETGAQ
                     DKPKEDVIIETIEVFD"
     gene            24041..24418
                     /locus_tag="VHD02_000873"
     CDS             24041..24418
                     /locus_tag="VHD02_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEQ5497492.1"
                     /translation="MKIGDKLHGTITGIKPYGAFVALDNGTTGLIHISEIKTGFIDDI
                     DQLLAIGNQVLVQVIDIDEYSKKASLSMRTLAEEKQHFFHRHRYSNSRHKIGFRPLEE
                     QLPQWIEESLQFLKKNTKKSLEP"
     gene            complement(24436..25377)
                     /gene="cysK"
                     /locus_tag="VHD02_000874"
     CDS             complement(24436..25377)
                     /gene="cysK"
                     /locus_tag="VHD02_000874"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEQ5497493.1"
                     /translation="MTKIYKTITELVGQTPIIKLNCLIPNEAADVYVKLEAFNPGSSV
                     KDRIALSMIEAAEAEGLISPGDVIIEPTSGNTGIGLAWVGAAKGYRVIIVMPETMSLE
                     RRQIIQAYGAELVLTPGAEGMKGAIAKAETLAIELGAWMPMQFNNPANPSIHEKTTAQ
                     EILEAFKEISLDAFVSGVGTGGTLSGVSHVLKKANPETAIYAVEAEESAVLSGQEPGP
                     HKIQGISAGFIPNTLDTKAYDQIIRVKSKDALETARLTGAKEGFLVGISSGAALYAAI
                     EVAKQLGKGKHVLTILPDNGERYLSTELYDVPVIKTK"
     gene            complement(25505..26137)
                     /locus_tag="VHD02_000875"
     CDS             complement(25505..26137)
                     /locus_tag="VHD02_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEQ5497494.1"
                     /translation="MEHFKTIKASGFFEESIKKSRFICHIKRVSTEEDGKNFVNAIKK
                     EHYKANHSCFAMIIGNNSQIKRSSDDGEPSGTAGIPILSVLEKQCLTNVVVVVTRYFG
                     GIKLGTGGLIRAYSNMTATAIKRFGIIEVKQQIGLEITLSYPQYQLYSNLLDQLALTE
                     TETKFSDTIKTTLYCDTERVENLIDTLTNYYHGQISCEKIGSKVIEFPCD"
     gene            26192..27516
                     /locus_tag="VHD02_000876"
                     /pseudo
     CDS             26192..27516
                     /locus_tag="VHD02_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880469.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            27488..28153
                     /locus_tag="VHD02_000877"
     CDS             27488..28153
                     /locus_tag="VHD02_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEQ5497495.1"
                     /translation="MICLLCQQISQTPISITEIIFLRRISSPICQQCQKSFQKIGKSV
                     CATCCANSDIIACRDCLKWENKGYNVNHRSLYCYNAAMKAYFSQYKFQGDYLLRKVFA
                     VELADVITKYYKGYIPVPVPVSPGCFRERQFNQVSAILEAANVSYLSLFEKLDNTHQS
                     SRTKKERLLVEKSYRLLKVSNIPDKILIVDDIYTTGSTIIALRKQLAKVANSDIKSLS
                     IAR"
     gene            28233..28781
                     /gene="raiA"
                     /locus_tag="VHD02_000878"
     CDS             28233..28781
                     /gene="raiA"
                     /locus_tag="VHD02_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEQ5497496.1"
                     /translation="MIKFSIRGENIEVTEAIRDYVESKLTKIEKYFAKDQEIDARVNL
                     KVYRERSSKVEVTIPLDSVTLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKHRE
                     KVPTGQVFTTEFEAEEVDEIPEVQVVRTKNVTLKPMDVEEARLQMELLGHDFFIYTDS
                     EDGATNILYRREDGNLGLIEAK"
BASE COUNT         9393 a         5181 c         5922 g         8568 t
ORIGIN      
        1 tttgagcaag aatttaagac tagtatgcga gggtttgata agaaagaagt tgatgaattt
       61 cttgataatg taattaaaga ttatgaaaat ttcaatgctc aaattgaagc tttaaaagct
      121 gagaacgaag ccttaaaaaa ggccaagttt caagctagaa atacagtttc agcgactgtt
      181 caacaaccag taccgcaacc gactcgtgtt gctcaatcag ctactaattt tgacatcttg
      241 aaacgtatta gtaaattgga aaaagaggtt tttggtaaac aaattattga gtaatgaaaa
      301 ctgtgcaatt tttggataat cgcgtagtat tttaatacta tgaggaaagt ccatgctagc
      361 actggctgtg atgccagtag tgattgtgct aggcgaacac ataagcctag ggatgtgcat
      421 acacattacg gcgaaggaaa tggctaagtc gtttgatatg ccaaagtact tctgaaagtg
      481 ccacagtgac gtagttttta tggaaacgta aaaaatggaa cgcggtaaac ccctcaagct
      541 agcaacccaa actttggtag gggcatggga tagttggaaa cgaacaagct atcctgactg
      601 tcaacagacg gtagacagat gattatcgaa ggaaataatt cctagttatt tccggaacaa
      661 aacatggctt atagaaaatt gcataatagg ttaagctagc acttgctagc ttttatttga
      721 tttaaaagaa agagatatat gaaagaaaca tttagattag tcgcaactgc agccgcaggt
      781 ttagaagctg tcgttggtaa agaagtcaga gcactaggtt ttgattgtca ggttgaaaat
      841 gggaaagttt attttgaagg tgatgtagaa gcgattgtta aaacaaatct ttggttacgt
      901 gcagcagatc gtatcaaaat tatcgtagga caatttcctg ctagaacctt tgaagaatta
      961 ttccaaggtg tctttgcctt ggattgggga aattatttac cgctaggagc taagtttcca
     1021 atctcaaaag ccaagtgtgt caagtcaaag ctacacaatg agccaagtgt tcaggcgatt
     1081 accaaaaaag ctgttgttaa gaaactgcaa aaacatttcc acaggccaga aggagttcct
     1141 cttcaagaag ttgggtcaac gtttaacatt gaggtatcta ttttaaaaga ccaagccact
     1201 attatgattg acacgacagg atctagccta tttaaacgag ggtaccgcgt tcaaaagggt
     1261 ggggctccta ttaaagaaaa tatggcagct gctatcttgg ccttatctaa ctggtttcca
     1321 gataaacctc tggttgatcc cacctgtgga tcgggaacgt tttgcatcga ggcagccctg
     1381 attgggatga atattgctcc aggatttaac cgttcgtttg cttttgaaga atggagctgg
     1441 gtagataaag acatggtgca acaagttcgt gatgatgccg aacaagaagc taattatgaa
     1501 atcgaattag atatttctgg ctttgatatc gatggccgca tgattgagat tgctaaaagt
     1561 aatgctgaag aagctggttt atctgatgtg attaccttta agcaaatgcg tctgcaagac
     1621 tttcgaacag acaaggttaa tggtgtagtg atatcaaatc ctccttatgg agaacggttg
     1681 cttgatgaca aagcggttga catcttgtat aatgaaatgg gacaaacctt tgcaccattg
     1741 aaaacatgga gcaaatttat ccttacaagt gatgaacttt ttgaactaaa atacgggcaa
     1801 aaagctgata aaaaacgtaa actttataac ggcaccctaa aagttgattt gtaccaattt
     1861 tatggggaaa gggtaaagcg acatctgata tagagaggta gtaagcgtgt cagaagaaag
     1921 caaagaagtc gaagtaacaa aagaatcgca aacattgggg cttaatgaag ccaagtcaat
     1981 gacaattggt gaagctgtac gcaaacagtc agagataaaa gcaggtgtta ctaaggatga
     2041 tagtatttta gataagtaca ttaagcaaca ccgtgacgaa gtttcttctc aaaagtttga
     2101 tgctaagtat acagagcttg atacagctag tttagataac tttatcaaaa aacaaagaga
     2161 agcattgagt aaagccggtc ttgttgatga cgaacctgtg tctgcggagt ctgctgagca
     2221 agatagcact ttggttgaag aagtagctga agacttagct ccaatggaga ctactgcggt
     2281 ggtaactggg attccggtgg aagcgacagt ccctgtgtta gatctagatc catcagagag
     2341 ggtcattcca gaaccacaga tgaccaagga agagccaaaa agagatcagt ttctttctga
     2401 agatagccac catccagcca aacaaaacac gaaaaaaggt tggttaatag ctttatttct
     2461 actattattg gccattcttg cagttgtatt tggttggaac catttcttga gacaagacag
     2521 tggtaaaaca actcaaacag caagtaaaca gacaaagacc tctttgcaga ccgacagtgc
     2581 taaaaaagca acgcgcttga aggctgctgc taaagctttc gaaaaacaat atggtacttt
     2641 ttatacagat gccacaaaga gcaaattaaa aaatagtgcc tttgctactt tgccagattt
     2701 agaagctgcc ttgaaagctt tggaaggctc tgcttactat gataaggcga aagcaaaagt
     2761 cgattccctt aagaaagcta ttgctgctat tacagcggtt aacggtaaat ttgtttccga
     2821 tgttgttgta gatggtgaaa aggtttcggc tgaagtcaag gctgatgcta attttgatga
     2881 tttatcaagt gcaaccttga caacaggaaa tgcaaatctt gatgctgtct tgcaggcaag
     2941 catcacagaa ggtcgtcagc aactagccag caaggctgag gccgctaaag cagctaatga
     3001 gcaagcagcc caggctcaag cagctcaagg acaaagtaca tcagtagcac catctggcta
     3061 tggcttgaca agttatgacc ctgcaagctt acaacggcat ttatcacgtg taccttacaa
     3121 tcaagatgtt attgctgaca gagctaaccc atcttgggca tttaatccag gagttcttga
     3181 aaaaattgta gcaacctcac aagctagagg ttatatttca ggctaccaat atattttaga
     3241 gccggttaat attattaatg gcaatggtta ctataatatg tttaagcctg atggcactta
     3301 tctgttctct attaattgta agacgggtta ttttgtagga aatggcaaag gttatgctga
     3361 tgctttagat tactgagaaa atcaaaaaaa actgagataa ggcaacttgt ctcagttttt
     3421 tgattaataa agtatgttgt cttaaaatct gcagatagac ttgttttttg taacaagtca
     3481 aataggtcag taggattgtt atgtttgaca acacactagt atcagatgac atggcgacta
     3541 aaaggatcgt ctgaaatccc ttgatcaata atcaattgag cccattcttt ggcggcaaag
     3601 aggctatgat ccttatagtt tccacaggat tcaagagttg ttccaggaac atcttcccaa
     3661 gtaatcccag ttgcaatttc ttctaggctg gatttaatca ccttggcaat atcagtagaa
     3721 ctgtgttttc cccacatgat aaggtgaaaa cctgttcgac agccaaaagg agagcaatca
     3781 atcatcccat caatgcgttg gcggatgagc ttggcaagta agtgttcaat ggtatgcaaa
     3841 ccggctgttt caatagaatt ttggttaggc tgcactaggc gaacatcaaa atttgtaata
     3901 cgatcgccct tgggtccaaa ttcttcagaa ataagacgaa cataaggggc ctttacaata
     3961 gtatgatcta gctcaaaact ttcgacgata acttcttttg tcatttcaat ctcctttgtt
     4021 ttacacttta tcataacaca aatagtgaga aagtggctac ttgtggagga gaaaaggcta
     4081 agaaaacaaa tcaaaggcta tcacaatgac ctgtgcgata aaagtactta cagaaacagt
     4141 aataaaagtt tctttaaaag catttttgtg gttaagtcct gtcaatgcta agaaggtgag
     4201 aaagacacca ctttggggta caatggtaaa gatattcgaa gcaatagtgg caacacggtg
     4261 aatcatttcg ggattaaggc cttgatctaa ataatactgg gcaaaattag gcatgacaat
     4321 tcctagagca cctgaagaag aaccggtaat agctgacata gaggaagtca acacggctaa
     4381 actaataaga ggattaccgg gaatattcaa aatgagatct gagaagaatg tgaaacctgg
     4441 ggcaatcatc acaacggctc cgaaggcaac tgcagatgct gtcgcaaaaa taggtgcaat
     4501 agagctgcta gctcctaaat taaagacagc aatcttatta ggaataaact gtctaaagag
     4561 ccagcttgcg gtgagaatgg cagctaaaag tgctatgaaa ataatgttct tcttaaaaaa
     4621 atcgtttccg aaaaggctac cggttagggc gataataatg agcaacaata aaggtaagat
     4681 actggcaagg aaatgtggga gattttttgt tttcacttgt atctcattgt caaaagcata
     4741 agtggcataa gtttcacccc ttgccatact ttttgctaga caatatttca tataaaagag
     4801 cccaaaagtc acgcagccaa ttgagccaac gatactcgga atggcggcag cagttagtga
     4861 ggtgtccaag tattgaatag gaataacatt ttgaatggca ggtgttccgg gtaaaatcgt
     4921 catggtaaaa gttgcaatac cgagccaaag aggaacttga attaagttct aagccaagtc
     4981 catttttttg aataggcttc gtgcaagcgg caagacagca aacatgacaa caaagaggct
     5041 gattccccca taagttaata tagcactgat cagaaaaata gcaacgagta ctttataagg
     5101 actatcatga ccaacttttt ttaaaatata atctgcaata gaagtggttg caccgcttgt
     5161 ttccattaat ttggctaaga tagaacctaa taaaaagata gcaaaataat tgagaatata
     5221 agtggagagt gcccccataa attggttggg ttcttttcca agtaaggttg tggttggatc
     5281 catttgatta aaccaaatga ccaagctggt agcaagaggc gcagcgatga taatgttaac
     5341 ttctttgaca gacaggtaaa taataacaat aacacctacc aagaccccca gagaaccaat
     5401 aatttccata gttttatgac ctccttatta gaaaataaag agacagatca caagatacag
     5461 cctctttatc tctccaaatt tattgagcag tgtagccacc gtctaagata caggcttgac
     5521 ctgtaacacc ttttgcctta tcactggcaa ggaaagacac atagtctgca atttcttgaa
     5581 cgtcaatgag gcgtttttga ggaacaagtg ggtatagcac ttcttcaaga acattttcaa
     5641 ggggaatacc tcttgttttg gaaaggtctt caaactggcc acgaaccagt ggtgtgtcaa
     5701 catatccagg acaaatggca ttgaccgtaa tgcctgaatc agcagcttct aaggcagtta
     5761 ctttggtcag accgatcaag ccgtgcttag ctgaattgta ggcggatttg ccagcaaaac
     5821 caatgacacc attgatagaa gccatattaa taatacgacc gtgtttttga gctttcattg
     5881 taggaaaagc acgtttaatg gcaataaatg gtgctgtcaa catgatttta atcatgaatt
     5941 caaatttttc agttggaaaa tcttcaatca tggcaacatg ttgaaggcct gcgttgttaa
     6001 ccaaaatatc aatacgacca tatttttcaa caaccgtatc aatagcagca ttgatggcta
     6061 cttctttggt gacatcgcac acaacactaa aagcgtcata gccttcttta gtatagtcag
     6121 caacaacctg atccagtttt tcttgattaa tgtcagaaaa aacaaccgtt ttaccttctt
     6181 ttaagaaggt aaaacggttg ttttccaatt ccgcttgcag caccagtgat aaatgcaact
     6241 tctttagtat gtgtcatgat actatcctcg tctttctttt ggttagtctt tagtttgttt
     6301 tatcaattag ggtaacttcg gtaacgtttt gcacgtcttc aaaggtgtaa tctgggttaa
     6361 tttcaagggc gcatagacct tcatcttgat attcaaagac tcccatttca gtaatgatta
     6421 ggttaacaac attttgagca gtcagaggaa gggtgcattt gtctaaaatt tttgctttgc
     6481 ctttattggt atgctccatt gcaacaatga cttttttagc acctaccaat aagtccatgg
     6541 cccctcccat cccaggaacc atttttccag gaatcaggta attagcaatg ctagcttctt
     6601 tatcaacttg taaggcacct aagacggttg cggccacatg gccgcctcga ataataccaa
     6661 aggaagttga gctatcgaag aaagcccctc caggagcaat actaacaggt tgcccaccag
     6721 cattaacaat actaggatcg tagtgatcat ctgttaatcc agtgagaccg acaaaaccat
     6781 tttcggactg cagggtaatg gttacacctt caggaatata gttggctact tttgttggca
     6841 aacctatgcc taagttgacc agtgtattgt gttctaattc ttgtgctaca cgctttgcaa
     6901 tacgtgtttg aatctcttct tttgaaagaa cagtagctga tgttcccatg ttaggctcct
     6961 ttcactaagt agttaacaaa aatgccagga gtatggacaa aattaggatc catttgcccc
     7021 acgtcaacaa cttctcttgc ttcaacaatg gtggttttcg cattagcagc catcacatga
     7081 ttaaagttat tttcagaacc tgcgtattgc aaattgccat ttttatctgc cttgttagca
     7141 aaaataagtg cgatatcagc tttcaaaggt ttttcaagaa ggtagtcttt gccgtcaata
     7201 gtaatgactt cttttccttt ggcaacttcg gttcctattc cagtaggtgt taagaaaccg
     7261 cctagcccaa aaccgccact gcggatgcgc tcagctaatg ttccctgagg tacaagatca
     7321 attacagtct cgccctcagt catttgtcgg cccgcttcac ggttgagacc gatatgtgat
     7381 gcaataatag ttgaaaactg tttattggca accatcttac caacgccttt gtcaggaaaa
     7441 ccggcatcgt tacagattaa agtgaggtct ttgacacctt tttccacaag tgcatcaatt
     7501 aatttttctg gtgtgccatt tgtcatgaag ccaccaacca taatggtatc accatcttta
     7561 acgtgagcta ccgcttcttt tattgtgatt tctttacaca ttttttctac caactttcac
     7621 gagactaagt attgtttttc acgataacag cagttccttg tcctccacca atgcaaagtg
     7681 ttgctagtcc tctggttact tggcgttttt tcatttcgtg gattaatgtg actagaatgc
     7741 gagcgccact ggcaccaata ggatgaccta aagcgatggc tcctccatta acattcacga
     7801 tttctgggtt taactttaaa tcttttaaaa ccgccaagga ctgtgctgca aatgcttcat
     7861 tggactccac taagtccaaa tcagaaattg ttaatccagc tttcttaagg gctttttgag
     7921 tggcaggaat tggtccagtt cccatcagtt caggtgcaac acctgcacta gcatagcttt
     7981 caatagtgac taaaggtgtt aaccctaatt caagagcttt ttcttcagtc attaacatca
     8041 ataaggctgc accatcgttt attcctgagg cattcccagc agtaacggtt ccttctttag
     8101 ggaggaaagc aggacgtaat tgctgtaatt tttcaaggct tgtgtcaact tttgggtatt
     8161 catccgtcgt aacaattaaa ggatctttgc gtcgttgagg cacagagaca gggacaattt
     8221 cgtcagcaaa acgatgattt tctaatgccc tagcagcttt ttcttggctt tctaaagcaa
     8281 attgatcttg ctcttctctt gagatgccat atttctgagc aacgttttca gcagtaatac
     8341 ccatgtggta attgttaaaa gcatctgtta agccatcatg aacaagagta tcaatcaatt
     8401 gactattacc aagacgttga ccaaagcggt gctgagttga gacataagct gcttgtgaca
     8461 tgttttcgac accaccagca ataacgatat cagcgtcacc aagcatgatg ctttgcgcag
     8521 ctaattggat tgctttaagt ccagaaccgc aaaccatgtc taatgtgaaa gctgttttat
     8581 cttcaggaat tcctgagtgg actgcaactt gtcttgccac attttgtcct aggcctgcat
     8641 gaaggacgtt tcctaggatg acttcatcaa ccatttcagg ctttacttgc gttttatcaa
     8701 ggattttttt cagtacagtt gttccaagtt caacagcaga gacactttta aaggagcctc
     8761 caaaagctcc gataggagtt cgtaaagctt caacgataac tatatttttc ataaaataaa
     8821 catcctttca gttttagaaa gcgctttcca tgtctctaag ataacaagaa aacgctaaaa
     8881 ataaaaatat agaattttta taagcgtata aatttttttg atagagttgg caatagcatt
     8941 taacatctac ttgtgtcata atataggtaa ttataccgga aaaagcaatt gataagacaa
     9001 ggagaatcct atttggatat taaacaaatt cgttatttta tcgccatcgt tgaaaatcat
     9061 tttaacttga gccaggcggc ggaactgttg tatgtgtcgc agccgacact tagcatgatg
     9121 atcaatgatt ttgaaaaaag ggaaaatgtt aagctcttca aacgaaaaag aggtcgtatt
     9181 attgggttaa cctatcttgg tgataattat tataaggacg ctcaaaaggt tctcagtctc
     9241 tatgatgaca tgtttttgaa attgcatgat cacagtaaag ggttaaaggg aagtatcaat
     9301 attggtattc ctcctcttat tttatcagtt gttttctcag aagtaatgtc aaaactgatt
     9361 ttagaaaatc cggggattca attcaatgtt aaggagattg gtgcttacca actaaaaaat
     9421 gaactactcg tgggaaatgt tgacgtcgca gtcttgttat ccccaacagg catcgctgat
     9481 aatttggttg agacttatga aatccagaga tcagaattat ctgtatgcct gtctccacgc
     9541 catcggttag cttccaaaaa agttattcag tggaaagatt taacagatga acaacttgcc
     9601 ctatttgatc ctagtttcat ggttcaccat ttagtgcttg aagcttgcga gcgtcaccaa
     9661 gtcagaccta atattatttt gacgtcctct tcctgggatt ttatgcttaa ttctaccaag
     9721 attaatcaca acgtcttaac tatttgtccc aaacctatta cagaattata ccaattaaag
     9781 gacattaagt gtattccaat ggaacgtccc atttcttggc gtgttgtctt aacacgcctc
     9841 cggaaaaaag ttattctgaa atagaagcct acatcatgga cgacttgcta cagtcttttt
     9901 ttaagtagga ttctcaacca cttggcccag atgttaattt ttttaaaaat gaattggaat
     9961 tgtgtaaacg ctcaatttat gataaactaa gaggtggctt tctattaatg aaatctagaa
    10021 tatattgagg tacaaacatg gttaatatta ttttattaat tgtttctgcc ctcattggtt
    10081 taatattagg ttatgcactt atttcgatta gactcaagtc tgcgaaggaa gctgcagagt
    10141 tgactctttt aaacgctgaa caagaagctg ttgatattcg tggcaaagca gaagtagatg
    10201 ctgaacacat caaaaaaaca gctaaacgtg aaagtaaagc aaatcgtaaa gaattacttt
    10261 tagaagcaaa agaagaggca agaaaatatc gtgaagagat tgaacaagaa tttaagtctg
    10321 aaagacaaga gcttaaacaa ctcgagacac gcttagcgga gcgctcctta actcttgacc
    10381 gtaaagatga aaacctatca agtaaagaaa aggtactaga tagtaaagaa caaagtctga
    10441 ccgataaatc taaacacatt gatgagcggc aacttcaagt ggaaaaactt gaagaggaga
    10501 aaaaagcaga actggaaaaa gttgctgcga tgacgattgc agaagcgcgt gaagtgattt
    10561 taatggagac ggaaaacaaa ctgacccatg aaattgcgac gcgcattcga gatgccgaac
    10621 gtgacatcaa ggaccgaaca gttaaaacag ccaaggactt gttagcgcaa gccatgcaac
    10681 gccttgctgg tgagtatgtg actgaacaaa ctattaccag tgtccatctc ccagacgaca
    10741 acatgaaggg ccgaattatt ggacgtgaag gccgtaatat tcgtacttta gagagcttga
    10801 ctggcattga cgttattatt gacgatactc ctgaagttgt tatcttgtca ggatttgatc
    10861 ctattcgacg tgaaattgct cgtatgacct tggaatctct gattgctgat ggtcgcatcc
    10921 atccagctcg tatcgaggaa ttggttgaga aaaatcgtct tgaaatggat aatcgtattc
    10981 gtgagtacgg tgaagctgca gcctatgaga ttggtgcacc aaaccttcat cctgatttga
    11041 ttaaaatcat gggacgcctg caattccgta cctcgtttgg tcaaaatgtc ctacgtcact
    11101 ctgttgaggt tggtaagtta gctggtattt tagctggtga gttaggtgaa aatgttgctc
    11161 ttgcccgccg tgctggtttc ttgcatgata tgggtaaagc tattgaccgt gaggttgaag
    11221 gtagtcacgt tgagattgga atggaatttg cacgtaaata caaagaacat ccagttgttg
    11281 tcaacactat tgctagccac cacggagatg tggagccaga ttctgtgatc gctgtgctag
    11341 tagctgcagc agacgctctc agttcggctc gcccaggcgc tcgtaatgag tcaatggaga
    11401 attacatcaa gcgtcttcgt gatttagaag aaatcgcgac aagttttgat ggggtacaaa
    11461 atagttttgc tctacaagct ggacgtgaga ttcgtattat ggttcaacct gaaaaaattt
    11521 cagatgatca ggttgtcatt ttgtcgcata aagtaagaga aaaaattgaa aacaatctag
    11581 attacccagg aaatattaaa gtaactgtta ttcgtgagat gagagcggtt gattatgcca
    11641 agtagaaaaa gaggaattat cctctttttc tttatgactt gcctttgaaa atgttcttga
    11701 aaaagactag cctttttgat aaactattaa aaagcaacta ataaggagaa tacaatgtct
    11761 gaacgcggtt tgttaatcgt cttttctggc ccttcaggtg tcggaaaagg aacggtaaga
    11821 caagaaattt tttcaacgcc agatcataaa tttgaatatt cggtttcaat gacgactcgt
    11881 ccgcaaagac cgggcgaggt tgatggggtt gattatttct tccgcacacg tgaagaattt
    11941 gaagaactca ttaaaactgg tcaaatgcta gagtatgctg aatatgttgg taattactat
    12001 ggaacccctt taacttatgt taatgagact cttgataagg gaattgatgt ctttttagaa
    12061 attgaagttc aaggagctct tcaggttaag tcaaaagtac cagatggtgt ttttgttttt
    12121 ttaacaccac cagatttaga tgaattagaa gaccgcctgg ttggtcgtgg tacagatagt
    12181 caagaagtca tagctcaacg tattgaaaga gcaaaagaag aaattgccct tatgcgcgaa
    12241 tacgattatg cagttgttaa tgatgaagtt gccttggcag ctgagcgggt taagcgtatc
    12301 attgagacag aacactttcg tgtggagcgc gtgattggtc gttatgataa aatgattaaa
    12361 ataacaaaaa atccctttaa agctaaatag aaaagagtaa gcattatgat gttaaaacct
    12421 tccattgata ccttattaga taaagtacca tctaagtatt cacttgtgat tttacaagcc
    12481 aaacgtgccc atgaactaga agctggtgcc actccaactc aagaatttaa atcagtgaag
    12541 tcaacacttc aggcacttga agaaattgaa tcaggaaacg ttgtgattca cccagatcct
    12601 tcagctaaac gtgaagctgt ccgtgctaag attgaggcag aacgcttggc caaagaggaa
    12661 gaggaacgta aaatcaaaga acaaattgcc aaagaaaaag aagaagaagg agaaaaaatc
    12721 taaggtttca ggaatcttag attttttcat atttagtacg atgtccatga cagaaaagga
    12781 gaagcaaatg gaaaaagtag ctcatgtgat tgtagatatt ccattgatgc aaacggacaa
    12841 gccattttct tatggtattc caaaagagtt agtttctctc gtgcagcttg gttctcgtgt
    12901 gcatgttcct tttggaaaag ggaatcgttt gttgcaaggc tttgttattg gttttggcca
    12961 ggaagattca agctctctta aactgattca gaccgtttta gatcctgagc cagtcctaaa
    13021 ccaagaacaa ttgacccttg ctgatcagct tcgaaaaaca gttttttctt ataaaatcac
    13081 gctcttaaaa gcaatgattc ctaatcttct taactctaac tatgataaag tgctaaggcc
    13141 agagtcaggt ctaaaaaagc ctgatagaga ctttcttttt gaaggaaaac catctgtctt
    13201 atattctacc ttagaccgcg agaaggaaaa aatagctctt aaggccatac aagctgggca
    13261 tattactgtt agttatttag ccaaagacaa gaaaaaccta aagacagaaa aatattatca
    13321 cgtcgactta gacgccttag cggtacatcc catctctagt cgtgccaaaa agcgtcaatt
    13381 attaaaagac tatctgctaa cccatacgaa ggaagctaaa cttgcaacgc tttatcaagc
    13441 cttttctcga gatgtggtgg cttactttgt gacaaatcac ttaattcgta ttgacgaacg
    13501 tcaaattgat cgcagtgagc cctattttga ccagatcaaa ccatcttctt ttttaacctt
    13561 aaatgaacag caagcgtcag ctgttactga aattgttgag caaattggga aaccgagtaa
    13621 accttttttg attgaaggga ttacaggttc gggtaagaca gaagtttatc ttcatatcat
    13681 cgaagcagtt ttaaaacagg ataagacagc cattgtttta gtaccagaaa tttcgttgac
    13741 tcctcagatg acctctcgtt ttatttcccg tttcggaaag caagtggcta ttatgcattc
    13801 aggcttatca gatggtgaaa agtttgatga gtggcgcaag ataaaaacag gacaggctaa
    13861 ggttgtggtt ggtgcccgct ctgctatttt ctccccacta gagcggattg gtgcaattat
    13921 tattgatgag gagcatgaat caacctataa acaagaaagc aaccctagat accatgccag
    13981 agaggttgcc ctgctcagag ctaaacacca tcaagcagtt gtggttatgg gctctgctac
    14041 gccaagtatt gaaagccgag caagggctag taagggggtc tatcatttta ttcaattaac
    14101 gcaaagagca aatcctcttg ctaagattcc agaagtaacg attgttgatt ttcgtgatta
    14161 tattggacag caagcagtta gcaattttac cccatatttg attgataaga ttaaagaacg
    14221 tttggttaaa aaagaacaag ttgtcttaat gttaaataga cgtggttatt ctagttttgt
    14281 gatgtgtcgg gactgtggtt atgtggacaa atgtcccaac tgtgatatct ccttaaccct
    14341 tcacatggat actaaaacta tgaattgcca ttactgtggg tttcaaaaac ctatcccaat
    14401 tacttgtcct gagtgtcata gcaacagtat ccgctattat ggcactggaa ctcaaaaagc
    14461 ttttgatgaa ttgcaagggg ttatcccaga agctaggatc ttgagaatgg atgttgacac
    14521 cacacgaaaa aaaagaagtc acaaaaccat actagatagt tttggtcgcc aggaagcaga
    14581 tattttgtta gggacgcaaa tgattgccaa aggactggat tttccaaatg tgactttagt
    14641 tggggtacta aatgcagata cctccttaaa tttgccagat tttcgagcgt cagaaaaaac
    14701 gtttcaattg ttaacacagg ttgctggtcg ggcaggccgt gcccataagc caggagaagt
    14761 cttgattcaa acttataatc ccgatcacta tgctattcaa ttagctaaga aacaagattt
    14821 tgaagccttt taccgttatg aaatggcgat tcgccgtcag atggcttatc ccccttacta
    14881 ctttacagtg ggtattacgc tttcgcatcg tttagaagca tcggttgtaa aaaaagctta
    14941 tcaagttaca gaactgctaa agagtcactt atcggataat attaaaatat taggaccgac
    15001 tcctaagcca attgctagaa cacacaattt ataccactat caaattttgt taaaataccg
    15061 ttttgaagat aacctagaag agacgctcaa tcgtattctt gattggagtc aagaagcaga
    15121 taatcgtcat ttaaaactaa ttattgattg tgaaccgcaa caatttttat aaggtaaatc
    15181 actaaatcta ggatgatcag ttatgatata ataaaaatac aaagaggaaa gtcatgataa
    15241 aattattatt tatgggaact ccccaatttt cagcaactgt attgaagggc ttgctagata
    15301 atcctgccta tgagatttta ggagtggtta ctcagcctga tcgagctgtt ggacgcaaaa
    15361 aagatattaa ggtaacacct gtaaagcaat tagctcttga gcatggaata tcaatctatc
    15421 agccggaaaa attatcaggc tctcaagaac tcattgagat tatgggactt ggtgcagatg
    15481 gtattattac agcagcattt ggccaatttt taccaacact attacttgat tcagtttcat
    15541 ttgctattaa tgtacatgca tcattgttac ctaaatatcg aggtggagca cctatccatt
    15601 atgctattat gaatggagat aaagaggctg gcgttactat tatggaaatg attaaagaaa
    15661 tggatgcagg tgacatggtc gctaaagcaa gcactccaat tcttgaaacc gataatgtag
    15721 gtactctttt tgagaaatta gccatcattg gtcgagactt acttttggat agtttgccag
    15781 cttatctttc tggagaatta aaacctattc ctcaagatca cagccaagct actttttcac
    15841 ctaatatctc tccagaacaa gaaaaattag attggacaat gtctaatcaa gaagtgttca
    15901 atcatattcg tggtatgaac ccttggccgg tagcgcatac gtttttagag gggcaacgtt
    15961 taaaaattta cgaagctcaa ctagctgaag gagaaggatt acctggacaa gttattgtca
    16021 aaaccaaaaa atctttggta atagctactg gacaaggagc tttgtccttg atcgttgtgc
    16081 aaccggctgg taaacctaaa atgtcaataa tagacttttt aaacggcatt ggtagaaaac
    16141 ttgaagtagg ggacatcatt ggcagataat tggaaaaaaa gtacgcgagg caaagccctc
    16201 cttgtcattg aagctatttt tgatcaggga gcttacacaa acatagcgct taatcagcag
    16261 ctaagcaata aagctttatc tgctaaggac agagctctat taactgagat tgtatatggt
    16321 accgtttcac gtaaaattag cttggaatgg tatttagccc attatgtcaa agaccgagat
    16381 aaattggaca aatgggttta ttatctcctt atgctcagtc tttatcaact gacttattta
    16441 gataagttac cagcgcatgc cattgttaac gatgcggttg gtattgccaa aaatcgtggc
    16501 aataaaaaag gtgcagagaa gtttgtcaat gctattttac gtcaattcac cagccatcct
    16561 ctcccagata tggaaacaat taagcgtcga aataagtatt attcggtcaa gtattcactg
    16621 cctgtttggt tggttaaaaa gttagaagac caatttggct cccacagagc agtggctatt
    16681 atggagagtt tatttgtgcg tagtaaggct agtattcgcg tgacggaccc tttgaagtta
    16741 gaagaagttg ctgaggcttt agatgcagaa cgttcccttt tatcagcaac tggtttaacc
    16801 aaagcgtcag gtcattttgc agccagtgat tatttcacaa atggggacat cactattcaa
    16861 gatgaaagca gtcagcttgt agctcctact ttaaacattg acggtgacga cataattctt
    16921 gatgcgtgta gcgctccagg gggtaagacc tctcatatag catcttactt aaaaacaggt
    16981 aaagttattg ccttagatct ctatgatcat aaactagagt tggtgaagga aaatgctaac
    17041 cgcttaggag ttgctgataa cattgagaca agaaaactag atgccagaga ggttcatcgt
    17101 cattttgaaa aggatagctt tgataagatt ttggtggatg ctccttgttc aggaattggt
    17161 ttgattcgtc gaaaacccga tatcaaatat aataaagaaa gtcaagattt taatgctcta
    17221 caggctatcc agctagagat attgtctagt gtttgtcaaa ccttgcgtaa aggtggtata
    17281 ataacataca gtacttgcac tatttttgat gaggagaatc gtcaagtcat cgaagcattt
    17341 ttacagagtc atcctaattt tgaacaagta aaactgaacc atacacaagc agatattgtc
    17401 aaggatggtt atcttattat cacaccagaa caataccaga ctgatggctt ctttattgga
    17461 caagtcagac gagtcttgta gtattttagg aggaaactga ctgagtcaga aaaaacgtat
    17521 gaagatttca ttaaaaacag acattgggca aaaacgctca aacaatcaag attttatcaa
    17581 taagtttgat aataaaaaag gcatcacttt agtgatttta gcagacggta tgggtggcca
    17641 cagagctgga aatattgcca gtgagatgac agtcacagac ctgggaagag aatgggtaaa
    17701 aacagacttc acagagttaa gtcagattag agattggtta tttgaaacta tccagtcaga
    17761 aaatcaacgg atttatgatc taggccaatc agaagacttt aaaggaatgg gaacgactgt
    17821 tgaagcagta gctttagttg aatctagcgc catttatgct catattggtg attcacggat
    17881 tggtcttgtc catgatgggc attatacctt attgaccagt gatcattctt tagtgaatga
    17941 gttggttaag gcaggacaga tcacagaaga agaagctgct agccatccac aaagaaatat
    18001 tatcactcaa tctattggtc aagcttcccc agtagagcct gatttaggag tgagagtttt
    18061 ggagccaggg gattatcttg tcattaacag tgatggtcta accaatatga tttcaaatga
    18121 tgaaattgtg actatcttgg gcagtaaggt tagtcttgat gaaaaaaacc aagaaatgat
    18181 tgacttagct aacctaagag gaggcctaga taatattaca atcgctttgg ttcacaacga
    18241 aagtgaggac gtcgaatgat tcagattggc aaattatttg ctggtcgtta tcgcattctg
    18301 aaatctattg gccgcggtgg tatggcggat gtttatttag caaatgattt gatcttggat
    18361 aatgaagacg ttgcaatcaa ggtcttgcgt accaattatc aaacagatca ggtagcagtt
    18421 gcgcgtttcc aacgagaagc gcgggccatg gctgaattga accatcccaa tattgttgcc
    18481 atccgggata taggtgaaga agacggacag caatttttag taatggaata tgtggatggt
    18541 gctgacctaa agagatacat tcaaaatcat gctccattat ctaataatga agtggttaga
    18601 attatggaag aagtcctttc tgctatgact ttagcccacc aaaaaggaat tgtacacaga
    18661 gatttaaaac ctcaaaatat cctactaact aaggagggtg ttgtcaaagt aactgatttc
    18721 ggcatcgcag tagcctttgc agaaacaagc ttgacacaaa ctaattcgat gttaggcagt
    18781 gttcattact tgtctccaga acaggctcgc ggctccaaag cgacgattca aagtgatatt
    18841 tatgcgatgg ggattatgct ctttgagatg ttgacaggcc atatccctta tgatggcgat
    18901 agtgctgtta cgattgcctt gcaacatttt caaaagcctc ttccatctat tatcgaggag
    18961 aaccacaatg tgccacaagc tttggagaat gttgttattc gagcaacagc caagaaatta
    19021 agcgatcgtt acgggtcaac ctttgaaatg agtcgtgact taatgacggc gcttagttat
    19081 aatcgtagtc gggagcgtaa gattatcttt gaggatgttg aaagtaccaa acccctcccc
    19141 aaagtggcct caggtcccac cgcttctgta aaattgtctc cccctacccc aacagtgtta
    19201 acacaggaaa gtcgattaga tcaaactaat caaacagatg ctttacagcc ccccaccaaa
    19261 aagaaaaaaa gtggtcgttt tttaggtact ttattcaaaa ttcttttttc tttctttatt
    19321 gtaggtgtag cactctttac ttatcttata ctaactaaac caacttctgt gaaagttcct
    19381 aatgtagcag gcactagtct taaagttgcc aaacaagaac tgtatgatgt tgggctaaaa
    19441 gtgggtaaaa tcaggcaaat tgagagtgat acggttgctg agggaaatgt agttagaaca
    19501 gatcctaaag caggaacagc taagaggcaa ggctcaagca ttacgcttta tgtgtcaatt
    19561 ggaaacaaag gttttgacat ggaaaactac aaaggactag attatcaaga agctatgaat
    19621 agtttgatag aaacttatgg tgttccaaaa tcaaaaatca aaattgagcg cattgtaact
    19681 aatgaatatc ctgaaaatac agtcatcagt caatcgccaa gtgcgggtga taaatttaat
    19741 ccaaacggaa agtctaaaat tacgctcagt gttgctgtta gtgatacgat cactatgcct
    19801 atggtaacag aatatagtta tgcagatgca gtcaatacct taacagcttt aggtatagat
    19861 gcatctagaa taaaagctta tgtgccaagc tctagctcag caacgggctt tgtgccaatt
    19921 cattctccta gttctaaagc tattgtcagt ggtcaatctc cttactatgg aacgtctttg
    19981 agtctgtctg ataaaggaga gattagtctt tacctttatc cagaagtaac acactcttct
    20041 agtagctcat cgagttcaac gtcaagttca aacagttctt caacaaatga tagtactgca
    20101 ccaggtagca acactgaatt aagcccatca gaaactactg ctcaaacacc ttaacaaaga
    20161 atagttagtc tcatgtttta ctagcatgcc actaactatt ctttttaact actcgtcata
    20221 taaagatgca attttccagg aattctgata aaatacaaag aaataagggg aaactaatga
    20281 aaaaatttca attcttttta cttatcgagt gtatactgct agctatgggt atcatgacaa
    20341 tcttggataa tgatttatca agttttatcc taattctagt tcttatctta ttagcattac
    20401 gcttttataa ccaagatagc cgcaataatt ttttactaac tgtcagttta ttgtttcttt
    20461 ttttaatttt catgcttaat ccctacatta tcatggctgt tcttttgggg atagtttaca
    20521 ttttcattaa ccatttttca caagtcaaaa agaagaatcg ctttgccttg attcgtttca
    20581 aggaagaaaa gattgaagtg aacaatacca agcatcaatg gattggtact gctaattatg
    20641 aaagtgatta ttattgtttt gatgacatta acatcattcg aatctcagga aatgacacgg
    20701 ttgacttaac caatgttatt gtgacaggaa tggataatat cattgtgata cgtaaaattt
    20761 ttggaaatac gactatattg gtacctattg atatcactgt tactttagat gttagttcca
    20821 tatacggaag cgttgatttt tttagatgtc agcaatatga tttgcgcaac gaatctatta
    20881 agttaaaaga aacggataac caatccctta aaaaagtcaa aattatcgtt acaactattg
    20941 ccggaaatgt tgaggtggat aggcgatgaa aaaacgttac tatgctcttg tttggctcta
    21001 ctcaaccatt accattttat ctattgtttt tgtggtcatg gataatttag gaatcacttt
    21061 taactacctt cgcaatcatt tatggcaggt cgaacgtcta gggttttcga ttttattgtt
    21121 gattgtttct gtgactttat tattgttgtt attgtggatt attatggatg ataacagtaa
    21181 gcgtaacatc aaccaaaatc taaaatatat tctcaataat cgacgactct atctagatga
    21241 gacatcagaa atcaatacta atttaagtcg actttctaaa aaaatgtctc acctgactgc
    21301 taacatgcaa aagaaagaaa gtgcttatat tcttgatagc caagaagttg taaaacaaga
    21361 gcgcaaacga attgcaagag atttacatga cacagtcagt caagagctat tcgcttcgtc
    21421 attgatttta tcaggaattt cgatgagttt ggaacaactg gacaaaacac aattacaaac
    21481 acagttaaca acggttgaag caatgttgca aaatgctcaa aatgatctac gcattctcct
    21541 tttgcatctt agacctaccg agctagctaa tcggacttta tctgagggcc ttaatatgat
    21601 tcttaaggaa ttaacagata aaagtgatat tgaagtcatt tataaggaaa ccattgctca
    21661 gcttcctaaa acaatggaag ataatctttt tagaattgcc caagaattca ttagcaacac
    21721 gttaaaacat gctaaagcta gtcgaattga agtttatctc aatcaaacct caacagaatt
    21781 acaattgaag atgattgatg atggtgtagg atttgatatg gatcaggtaa gggatttgag
    21841 ttatggtctg aagaatattg aagaccgtgt caatgattta gcaggaaacc tacatttaat
    21901 tagtcaaaaa ggcaaaggag tttccatgga tattagactg ccgatagtga agggagatga
    21961 cgatgagtaa gataaaagtg atattggtcg atgatcatga aatggtccgc atgggactca
    22021 agagtttttt gaatttacaa gctgatattg atgtcgttgg tgaagcctct aatggacgtg
    22081 aaggggttga tttggcattg gccttgaagc cagatgtttt ggttatggat ctagtgatgc
    22141 cagagttagg cggtgttgag gcaactttag aagtcctaaa aaaatggaaa gaggctaagg
    22201 tacttgtgtt aacttcctat ctagataatg aaaagatata ccctgtcatt gatgctggtg
    22261 ctaaaggtta catgttaaag acttctagtg cagccgaaat tctaaatgct attcggaaag
    22321 tttcaaaagg tgaattggcc attgaaacag aggtggacaa aaaaattaaa gcgcatgacc
    22381 aacaccctga tttgcatgaa gagctaaccg cgcgcgagta tgatatttta catcttttag
    22441 ctaaagggta tgataatcaa actatagcgg atgaactttt catctccttg aaaaccgtta
    22501 aaacgcatgt atctaatatt cttgctaagc tagaagttga tgaccgaact caagcggttg
    22561 tctatgcttt tcgacaccat ttagtccccc aagacgataa ttaatgctat aatatagggt
    22621 atagcaaatt ggaggaaagt atggacgcaa aactaaaata caaagctaaa aaaatcaaaa
    22681 tggtgttctt tgatattgat gataccttga gggtcaaaga tacgggttat atgcctgagt
    22741 cgattcaaag agtatttaag gctttgaagg caaaaggcat cttggttggt attgcttcag
    22801 gtcgcgctcg gtacggtgtt ccacaagaag ttcaagattt acatgctgac tattgtgtca
    22861 aattaaatgg agcttatgtc aaagacgatg ctaaaactat tattttccaa gctccgattc
    22921 cagcagatgt tgttgtggcc tataaaaaat gggcggatga tatgggcatt ttttatggaa
    22981 tggcgggtcg tcatgaagcg gtgttgtccg ctcgaaatga catgattagc aatgctattg
    23041 ataatgtcta cgctcaacta gaggtctgtc cggattataa tgagtatcat gatgtttacc
    23101 aaatgtggac cttcgaggac aagggagatg gcttgcagtt gcctgctgaa ttagcagagc
    23161 accttcgttt ggtcagatgg cacgataact catcagatgt tgtcttgaaa ggtacgtcaa
    23221 aagcgctagg tgtttcaaaa gtagttgacc atttaggatt aaaacctgaa aacattttgg
    23281 tgtttggtga tgaactaaac gacttagagt tatttgacta tgctggtatc agtattgcaa
    23341 tgggagtatc acacccacgt ttgcaagaaa aagcagatgt tatcacaaaa aaagttgaag
    23401 aagatggcat tttatatgcc ttggaggaat taggattgat tgacaaagaa ttacagtttc
    23461 cacaattgga tttagaaaat catactggac caaaagtaac cattaaaaca aatcatgggg
    23521 atatgacttt ggtcttgttc ccagagcatt cccctaagac ggttgctaac ttcttaggat
    23581 tagctaaaga gggatactat gatggcatta ttttccaccg cgttattcct gaatttatga
    23641 ttcaaggtgg tgatccaaca ggaacaggaa tgggtggaca atccatctat ggtgaaagtt
    23701 ttgaagatga attttcggat gaactgtata accttagagg agccctttca atggcaaacg
    23761 caggcccaaa tactaatggt agccaattct tcattgttca aaatagcaaa attccttacg
    23821 ctaaaaaaga attagaacgt ggtggttggc ctactccaat tgcagctgct tatgctgaaa
    23881 atggtggaac tcctcacctt gatcggcgtc atactgtttt tggccaatta gctgatgacg
    23941 cttctttcaa agttttggac ctgattgcta gcgttgaaac tggcgcacag gataaaccta
    24001 aagaagacgt tatcattgaa acaattgagg tatttgacta atgaaaattg gcgacaaact
    24061 gcatggcacc atcactggga ttaaaccata cggagccttt gttgcccttg acaatgggac
    24121 aacaggtctc attcatattt cagaaataaa aacgggattt attgatgata ttgatcaatt
    24181 attggctata ggcaaccagg ttttagtaca agtaattgat attgatgaat atagtaaaaa
    24241 ggcaagttta tctatgcgga ccttagcaga agaaaaacag cattttttcc atcgtcatcg
    24301 ttattccaac agtcggcaca agattggctt taggccactt gaagagcagt tacctcagtg
    24361 gattgaagaa agcctgcagt ttttaaaaaa gaacactaaa aagagtctgg aaccttagta
    24421 gttccaaacc cttttttatt tcgtcttaat tactggtaca tcatagagtt cagtcgataa
    24481 atagcgttcg ccattatctg gtaaaatagt taacacatgt ttgccttttc ctaactgttt
    24541 agcgacttca atagcggcgt aaagagcagc tccagaagaa atcccaacca ggaagccttc
    24601 cttagctcct gttagtcgag cagtttctaa agcatctttc gatttaacac ggataatttg
    24661 gtcataggct ttggtatcta acgtgtttgg gataaatcca gctgatatac cttgaatttt
    24721 atgtggtcca ggctcttgac cagataagac agcagattcc tcagcttcaa cagcatagat
    24781 agcagtttca gggttagctt ttttcaagac atgtgaaaca ccagaaagtg ttcctccagt
    24841 accaacacca gatacgaatg catctaaaga aatctcctta aaagcttcca aaatttcttg
    24901 agctgttgtt ttttcatgga tgcttggatt ggcagggtta ttaaattgca taggcatcca
    24961 agcacctagt tctattgcta aagtttcagc ttttgcaata gcccctttca taccttctgc
    25021 tccaggtgtt aagacaagct ctgcaccata agcctgaatg atttgtcgtc tttccaagct
    25081 catagtttcg ggcataacaa taatgactcg atacccttta gcagcaccta cccatgcaag
    25141 accaatacct gtattaccgc ttgttggttc gataataacg tcaccaggac ttatcagacc
    25201 ttcagcttca gcagcttcaa tcatcgataa agcaatacga tctttaacag aagatcctgg
    25261 gttaaaagct tctaatttta cataaacgtc agcagcttcg tttggaatta aacagttaag
    25321 tttgataata ggtgtttgac ctactaattc tgttatagtt ttgtaaattt tagtcatagt
    25381 ttagcctctt ttcttgatac aaaataaata cgctgtgtat ttaagtataa ggagagatat
    25441 tattaaagta aaatacagat ttactatcac tctgataata aagccatatc acttttgttg
    25501 atgattaatc acaggggaat tcaataactt tggatccgat tttctcacag cttatctgac
    25561 catgataata atttgttaaa gtatcaatga gattttccac acgttcagta tcacaataaa
    25621 gtgttgtctt tatcgtgtct gaaaacttag tttctgtttc ggttaaagcc aattgatcta
    25681 gcaagttaga gtatagttgg tattggggat aagaaagtgt gatttctagt cctatctgct
    25741 gtttaacttc aatgatgcca aatcgtttaa tagctgttgc ggtcatgtta gagtatgctc
    25801 tgattaaccc acctgttcct aatttaatac caccaaagta acgcgttacc acaactacta
    25861 catttgttaa acactgtttt tctaacacag aaaggatagg tattccagca gttcctgaag
    25921 gttcaccatc atcacttgag cgcttgatct gactgttgtt accgataatc atcgcaaagc
    25981 aggaatgatt agctttgtaa tgttcttttt tgatagcatt aacaaaattc ttaccatctt
    26041 cttcagtgct cactcgtttg atatgacaaa tgaaacgaga ttttttaata ctttcttcga
    26101 aaaaaccact ggctttgatt gtcttaaaat gttccataaa aaaattgtac aagaaaagat
    26161 aaaaattagc aaaaaaagcg aataataaag tatggagtct attgaaaata gttatgggcg
    26221 gttgttgcta gaaaaccagt taccagattc agcaaggcag cttgctcaac ctttaaaaag
    26281 tgtagtaata tcgagaggaa aaatgatttg tcaacgttgt cactatcagc ttgatgaaga
    26341 agcacgattg ccaagtggag cctattattg ccgattttgt cttgtctttg gacgaaatca
    26401 atcaaataaa ttgctctatg ctatcccacc tatgcatttt ccaaaaggta attatctggt
    26461 ttggagcggt caactaactg cttatcaaga gatgatttcc caacaattat taatcaatat
    26521 gcaaaatcaa aaaacaacat tagttcatgc tgtaactggt gcaggaaaaa cagaaatgat
    26581 ttatgctgcg attgaggcgg taattaacac aggtggctgg gtttgcattg ccagcccaag
    26641 agtagatgtt tgtgtagagg tggctactcg tttatcccaa gccttctcct gcagcatctg
    26701 tttaatgcac gcagagtctc ttccttatca aagagctcct attatagtag ccacaacgca
    26761 tcagctgcta aaatttcaca aggcttttga tctcttaatt attgatgaag tagacgcatt
    26821 cccttttgta aataatattc aacttcatta tgctgcaagt caggctttaa aagaaggtgg
    26881 cgcaaagatt ttattaactg caacttcaac tagaacttta gaaagaaagg taaacaaggg
    26941 agaagtagtc aagctgacat tggcaagacg ctttcataat aggccattag ttattccgaa
    27001 atttataaga agttttaatc tatttaaaat gatacatcgg caaaaacttc ctcctaaaat
    27061 tttaaaatac ttaaaaaaac aaagaaaaac aggctatccc cttttaattt ttctacccac
    27121 tattataatg gcagaatctg ttacagcaat cttaaaagag ttattgcccg ccgaacaaat
    27181 agcttgtgtt tctagtcaat cacaaaacag gaaagaagat atcacagctt ttcgtcaagg
    27241 aaaaaaacga ttttaattac aacaacaatt ttagaaaggg gtgtgacatt tccccaaatt
    27301 gatgtttttg ttcttgggtc tcatcatcgt gtctatagtt cccaaagtct tgttcaaatt
    27361 gctggacgag ttggaagatc tatagacaga ccagacggaa cgttatattt ctttcatgaa
    27421 ggtatcagta aagcaatgct actagcaaga aaagagatta aagaaatgaa ttataaaggg
    27481 tattcgcatg atctgtctac tatgtcaaca aattagtcaa acaccaataa gtattacaga
    27541 aatcatcttt ttaaggcgta tctcttcacc gatttgtcaa caatgtcaaa aaagctttca
    27601 aaagatagga aaaagtgttt gtgcgacatg ttgtgcaaac tcagatataa tagcttgtcg
    27661 agattgtcta aaatgggaaa acaaaggata caatgtaaat catagaagct tatattgtta
    27721 taatgctgct atgaaagcat acttcagtca atataagttt caaggagact atttattaag
    27781 aaaagttttt gcagtagaac ttgccgatgt tattaccaag tactataaag gctatatccc
    27841 agtcccggtt cctgtaagtc ccggttgttt tcgagaaaga caatttaatc aagtgagcgc
    27901 tattcttgag gcagctaatg ttagctacct ttctcttttt gaaaagctag ataatactca
    27961 ccaatcttcc agaacaaaaa aagagagatt attagtagaa aaatcttatc gactactaaa
    28021 agtatcaaac attcctgata aaatccttat agtagatgat atttatacta ctggtagtac
    28081 aattatcgct cttagaaaac aattggctaa agtagcaaat agtgacatta aaagtttgtc
    28141 aattgcacgt taaaacttgc aaattttcat gaaagcagta taatagtatt aaagataaag
    28201 taagcgcttc aagcgcgaga aagaggtatc ctatgattaa gttcagtatt cgtggagaaa
    28261 atatcgaagt gacagaagct attcgcgatt atgtagaatc aaagcttact aaaatcgaaa
    28321 aatattttgc taaagaccaa gaaatagatg cacgtgttaa tttaaaagtt tatcgggaga
    28381 gatcttcaaa agttgaggta acgatcccat tagattcagt aacccttcgt gctgaagatg
    28441 tttcacagga tatgtacggc tctattgatt tagttgttga taagatcgaa cgccaaatta
    28501 gaaaaaataa aaccaaaatt gctaaaaaac atcgcgaaaa ggttccaact ggtcaagtct
    28561 ttacaactga gtttgaagct gaagaagtcg atgagatacc agaagtacaa gttgtacgta
    28621 ctaaaaatgt gacattaaaa ccaatggatg ttgaagaagc gcgtttacag atggaactat
    28681 tgggacatga tttctttatc tacactgatt ctgaagatgg tgcaacaaac attttatacc
    28741 gtcgcgaaga tggaaaccta ggattaatag aagctaaata gttcacaact aaaacctaca
    28801 gggatgatta tacgtatttt aaaagtggga aaaagctatt catgtttcga ggtgacatga
    28861 tgaaaatgag attaagtgat agatacgaca gtcattatat aacaaaagta tctattacag
    28921 ttcctcttta agtaaatctt acgtaaaatt agtttgaaaa aaagattagt cccaattctg
    28981 ggctagaaat tctcaaaaaa tatgaaaaag cgcgttttca gaatataaag cgcgtttttt
    29041 tctttttcca tttaacaata aaaa
//
LOCUS       DAUDYX010000020        28625 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.020, whole genome shotgun sequence.
ACCESSION   DAUDYX010000020 DAUDYX010000000
VERSION     DAUDYX010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28625
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.020"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(<1..200)
                     /locus_tag="VHD02_000879"
     CDS             complement(<1..200)
                     /locus_tag="VHD02_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3644 domain-containing protein"
                     /protein_id="HEQ5497497.1"
                     /translation="MENLSERLVLKSVEAFIMGLEIYNKPTIKYRIEGFSFFICNAWE
                     LMLKAEMLNRGFSIYYSDNPERT"
     gene            812..1048
                     /locus_tag="VHD02_000880"
     CDS             812..1048
                     /locus_tag="VHD02_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEQ5497498.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTLEVDGKVLQAT
                     PYFLIHVTGAGEGFSMWAPTPCDVLAEDWIEVND"
     gene            1041..1739
                     /locus_tag="VHD02_000881"
     CDS             1041..1739
                     /locus_tag="VHD02_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEQ5497499.1"
                     /translation="MTKVVLVTGCASGIGCAQARYFLRQGYHVYGVDKSDKPDLNGNF
                     HFIKLDLSSELSPLFKVVPSVDILCNTAGILDAYKPLLDVSDEEVEHLFDINFFATVK
                     LTRHYLRRMVEKQSGVIINMCSIASFIAGGGGVAYTSSKHALAGFTRQLALDYAKDQI
                     HIFGIAPGAVKTAMTASDFEPGGLADWVARETPIGRWTEPDEVAELTGFLASGKARSM
                     QGEIVKIDGGWSLK"
     gene            1815..2774
                     /locus_tag="VHD02_000882"
     CDS             1815..2774
                     /locus_tag="VHD02_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEQ5497500.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIDGIDLVAILSTVRSLKTAKDLA
                     KAYHMPLATSKYEAVLGNEEIDTVYIGLPNHLHFAYAKEALLAGKHVICEKPFTMTAG
                     ELDELVVIAKKRKLILLEAITNQYLSNMTFIKEHLDQLGDIKIVECNYSQYSSRYDAF
                     KRGDIAPAFNPKMGGGALRDLNIYNIHFVVGLFGRPKTVQYLANVEKGIDTSGMLVMD
                     YEQFKVVCIGAKDCTAEIKSTIQGNKGSLAVLGATNTLPQVQLSLHGQKPQVINHNKH
                     DHQMYEEFVAFKDMIDQKDFKKVNQALEHSRAVMAVLERAVHS"
     gene            3107..4444
                     /locus_tag="VHD02_000883"
     CDS             3107..4444
                     /locus_tag="VHD02_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ5497501.1"
                     /translation="MEEDYNKREPEKFTQFLRRQKVVFFVAFFGYVCAYLVRNNFKLM
                     SNTIMVQNGWDKAQIATLLSCLTVSYGLAKFYMGALGDRVSLRKLFSISLGASALICI
                     LIGFFNSSMVVLGILLVLCGVVQGALAPASQAMIANYFPNKTRGGAIAGWNISQNMGS
                     ALLPLTIALLTSMGLVVPANGNILLAFLIPGVLVFLFALCCWKLGGDNPESEGLDSLR
                     TMYGDAGESAVASEEEKHNLSYWQLIWKYVFCNPSLLLVAAVNVALYFVRFGIEDWMP
                     IYLSQVANMSEAHIHFAISILEWVAIPGSLVFAWLAVRYPNKMAKIGAIGLFVLAAIV
                     FVYERLTATGAPNYFLLLVIAGILGSLIYGPQLIVNILTINFVPLNVAGTAIGFVGVT
                     AYLIGNMGANWLMPILADGFGWFWSYIVVAALSAFSAVGYLILAKREEEIIKD"
     gene            4618..6000
                     /gene="glmU"
                     /locus_tag="VHD02_000884"
     CDS             4618..6000
                     /gene="glmU"
                     /locus_tag="VHD02_000884"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEQ5497502.1"
                     /translation="MTNYAIILAAGKGTRMTSDLPKVLHKVSGLTMLEHVFRSVKAIS
                     PEKAVTVIGHKSEKVRAVLADQSAFVHQTEQLGTGHAVMMAETQLEGLEGHTLVIAGD
                     TPLITGESLKSLIDFHVNHKNVATILTATAQDPFGYGRIVRNKDGEVIKIVEQKDANE
                     YEQQLKEINTGTYVFDNKRLFEALKCITTNNAQGEYYLTDVVAIFRANKEKVGAYILR
                     DFNESLGVNDRVALATAETVMRQRITQKHMVNGVTFQNPETVYIESDVEIAPDVLIEG
                     NVTLKGRTHIGSGTVLTNGTYIVDSEIGQGSIITNSMIESSVLAAGVTVGPYAHLRPG
                     TTLGREVHIGNFVEVKGSHIGEKTKAGHLTYIGNAQVGSSVNVGAGTITVNYDGQNKY
                     ETVIGDHAFIGSNSTLIAPLEVGDNALTAAGSTISKTVPADSIVIGRSRQVTKEGYAK
                     RLAHHPSRSK"
     gene            6034..6588
                     /locus_tag="VHD02_000885"
     CDS             6034..6588
                     /locus_tag="VHD02_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEQ5497503.1"
                     /translation="MKFEEKTLKRQTVFDGHIFKVVVDDVELPNNLGQSKRELIFHRG
                     AVAVLAITPERKIVLVKQYRKAIESVSYEIPAGKLEIGEEGSKLKAAARELEEETAYT
                     GTLTFLYEFYTAIGFCNEKITLFLATDLTQVANPKPQDDDEVIEVLELTYQECMDLVA
                     QGKLADAKTLIALQYYALHFGGEQ"
     gene            6588..6839
                     /locus_tag="VHD02_000886"
     CDS             6588..6839
                     /locus_tag="VHD02_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497504.1"
                     /translation="MGRPLLTDDIIEKARRMETFEPDDAVDFDTKVMTLPEKDEKARI
                     YKSRRIENAKRSQLQSKLNVILIAVMLLIAILVYAIFYL"
     gene            6859..7554
                     /locus_tag="VHD02_000887"
     CDS             6859..7554
                     /locus_tag="VHD02_000887"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEQ5497505.1"
                     /translation="MKIGIIAAMEEELSLLLANLLDAQEHQVLSKTYYTGRFGKHELI
                     LVQSGVGKVMSAMTVAILAEHFKAQAIINTGSAGAVASHLAIGDVVVADRLVYHDVDA
                     TAFGYAYGQMAGQPLYYDCDPQFVAIFKQVLKHEKTNGQVGLIATGDSFVAGQDKIDQ
                     IKTAFSDVLAVEMEGAAIAQAAHTAGKPFIVVRAMSDTAAHDANITFDQFIIEAGKRS
                     AQILMTFLENLPV"
     gene            7667..7885
                     /locus_tag="VHD02_000888"
     CDS             7667..7885
                     /locus_tag="VHD02_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497506.1"
                     /translation="MTGPKSHLQYSKDNTKLQLRNILNGYQSDLGRHYSSYYYYNLRT
                     VMGLSSEQEIEEHYEKLKNKLHDMYNHY"
     gene            complement(7982..8629)
                     /locus_tag="VHD02_000889"
     CDS             complement(7982..8629)
                     /locus_tag="VHD02_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEQ5497507.1"
                     /translation="MTPNKEDYLKCIYEIGEQEPKITNKMVAEKMHVSAPAVSEMIKK
                     MISQGWIVKDKAKGYLLKDKGYALVANLYRKHRLIEVFLIHQLGYNTQEVHQEAEVLE
                     HTVSDTFIDRLDKILDFPDFCPHGGTIPRYGQPLVEMNTTTLNTITELGRFRLSRIHD
                     HFDLIQYLEAHHLSINTELTLTQIDTFAKTYTICYGDKELVIPENIAKQLYVTAL"
     gene            8775..9707
                     /locus_tag="VHD02_000890"
     CDS             8775..9707
                     /locus_tag="VHD02_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_101244918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEQ5497508.1"
                     /translation="MGKRMSLILGAFLSVFLLVACSSTGTKTAKSDKLKVVATNSIIA
                     DMTKAIAGDKIDLHSIVPIGQDPHEYGPLPEDVEKTSNADVIFYNGINLEDGGQAWFT
                     KLVKNAQKTKNKDYFAVSDGIDVIYLEGASEKGKEDPHAWLNLENGIIYSKNIAKQLI
                     AKDPKNKETYEKNLKAYVAKLEKLDKEAKSKFDAIAENKKLIVTSEGCFKYFSKAYGV
                     PSAYIWEINTEEEGTPDQISSLIEKLKVIKPSALFVESSVDRRPMETVSKDSGIPIYS
                     EIFTDSIAKKGKPGDSYYAMMKWNLDKISEGLAK"
     gene            9771..10496
                     /locus_tag="VHD02_000891"
     CDS             9771..10496
                     /locus_tag="VHD02_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEQ5497509.1"
                     /translation="MITTNNLCVTYDGNSNALEAINVTIEGPSIVGIIGPNGAGKSTF
                     MKAILNLIDYQGHVTVDGKDGRKLGHTVAYVEQRSMIDYNFPITVKECVALGTYSKLG
                     LFRRVGKKQFEQVDKVLKQVGLEDFGHRPIKSLSGGQFQRMLVARCLIQESDYIFLDE
                     PFVGIDSVSEKIIVDLLKELKMAGKTILIVHHDLSKVEHYFDKLMILNKHLVAYGNVC
                     EVFTVDTLSKAYGNHLILGKEMV"
     gene            10497..11351
                     /locus_tag="VHD02_000892"
     CDS             10497..11351
                     /locus_tag="VHD02_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEQ5497510.1"
                     /translation="MSMKFFEGLMSYHFLQNALITAVVIGIVSGAVGCFIILRSMSLM
                     GDAISHAVLPGVALSFILGVNFFIGAIIFGLLASVIITYIKENSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDSDKWITIGVSIFVLVVISLFFKELLLT
                     SFDPILAKSMGVKVNAYHYLLMVLLTLVAVTAMQSVGTILIVALLITPAATAYLYANS
                     LKVMLVMSSLLGALASVLGLYLGYTFNVAAGSSIVLTSAMMFLISFFVSPKQGYLKRW
                     MQKKEKTP"
     gene            complement(11499..12305)
                     /locus_tag="VHD02_000893"
     CDS             complement(11499..12305)
                     /locus_tag="VHD02_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEQ5497511.1"
                     /translation="MKKLLSLSLVAISLLSLSACESVDRAIKGDKYIDEKTAKEESEA
                     ASKAYEESIQKALKADASQFPQLTKEVGKEEAKVVMRTSQGDITLKLFPKYAPLAVEN
                     FLTHAKKGYYDNLTFHRVINDFMIQSGDPKGDGTGGESIWKGKDPKKDAGNGFVNEIS
                     PFLYHIRGALAMANAGANTNGSQFYINQNKKNQSKGLSSTNYPKPIISAYEHGGNPSL
                     DGGYTVFGQVIDGMDVVDKIAATSINQNDKPEQDITITSIDIVKDYRFKN"
     gene            12522..14927
                     /locus_tag="VHD02_000894"
     CDS             12522..14927
                     /locus_tag="VHD02_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEQ5497512.1"
                     /translation="MVKRNQRRKSAPKKRLTKAEVEKQRAIKRMILSVLMALLLIFAM
                     LRLGVFGVTTYNMIRFLVGSLAYPFMFAWLIYLFCFKWLRQKDGMIAGVVIAFLGLLV
                     EWHAFLFAMPRMFDQDIFLSTARLITRDLLALRVTEFVGGGMLGALLYKPIAFLFSNI
                     GSYFIGFLFILLGLFLMTPWDIYDVSHFVKEAVDKLAVAYQENKEKRFIKREEHRLQA
                     EKEALEKQAQEEEKRLAELTVDPETGEIVEDSQSQVSYDLAEDMTKEPEILAYDSHLK
                     DDEASLFDQEDLAYAHEKIGAYDSLSALASSEDEMDMDEPVEVDFTPKTHLLYKLPTI
                     DLFAPDKPKNQSKEKNLVRKNIKVLEDTFQSFGIDVKVERAEIGPSVTKYEIKPAVGV
                     RVNRISNLADDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSFRELWEQSDANP
                     ENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISSILMKARPDQVK
                     FLMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFSKVGVRNIA
                     GYNAKVEDYNRQSEQKQMPLPLIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGIH
                     MILATQRPSVDVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKLLGRGDMLFKPI
                     DENHPVRLQGSFISDDDVERIVNFIKDQAEADYDDAFDPGEVSDNDPGFSGNGGAAEG
                     DPLFEEAKALVLETQKASASMIQRRLSVGFNRATRLMDELEEAGVIGPAEGTKPRKVL
                     QTN"
     gene            complement(14997..15350)
                     /locus_tag="VHD02_000895"
     CDS             complement(14997..15350)
                     /locus_tag="VHD02_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEQ5497513.1"
                     /translation="MMIYKLTALAFLIVTPLFAMIVTPLFKLNKKGFKFPDVALLLFA
                     IEIVLVSGKFFTHNLLPYYLIIMSLLAIVISLLLVIRTQSFSYHRFMKLFWRVGFLVT
                     VIFYLILVIFIFTLA"
     gene            15594..16019
                     /gene="rplK"
                     /locus_tag="VHD02_000896"
     CDS             15594..16019
                     /gene="rplK"
                     /locus_tag="VHD02_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEQ5497514.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGTPNTTKVATVTR
                     AQVQEIAETKMPDLNAANIEAAMRMIEGTARSMGFTVTD"
     gene            16125..16814
                     /gene="rplA"
                     /locus_tag="VHD02_000897"
     CDS             16125..16814
                     /gene="rplA"
                     /locus_tag="VHD02_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEQ5497515.1"
                     /translation="MAKKSKQMRAALEKVDSTKAYSVEEAVALVKETNFAKFDASVEV
                     AYNLNIDVRKADQQIRGAMVLPNGTGKTQRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINGGWLDFDVVIATPDMMAIVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDADKLVENFKAFHDVMAKAKPATAKGTYMANVSITSTQ
                     GVGIKVDPNSL"
     gene            17136..17864
                     /locus_tag="VHD02_000898"
     CDS             17136..17864
                     /locus_tag="VHD02_000898"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEQ5497516.1"
                     /translation="MEPKYQRILIKLSGEALAGEKGVGIDIPTVQAIAKEIAEVHVSG
                     VQIALVIGGGNLWRGEPAADAGMDRVQADYTGMLGTVMNALVMADSLQHYGVDTRVQT
                     AIPMQNVAEPYIRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEADAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHGEVIKRGLKIMDATASTLSMDNDIDLVVFNMN
                     EAGNIQRVVFGEHIGTTVSNKVCD"
     gene            17893..18450
                     /gene="frr"
                     /locus_tag="VHD02_000899"
     CDS             17893..18450
                     /gene="frr"
                     /locus_tag="VHD02_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEQ5497517.1"
                     /translation="MANAIIETAKERFAQSHQSLSREYASIRAGRANASLLDRIQVDY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRKELAKEVKKVGENAKIAIRNIRRDAMDDAKKQEKAKEITEDELKTLEKDI
                     QKATDDAIKEIDRMTAEKEKELLSV"
     gene            18559..19416
                     /locus_tag="VHD02_000900"
     CDS             18559..19416
                     /locus_tag="VHD02_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEQ5497518.1"
                     /translation="MNDLLATVITGLIKEENANDYFIQKEGFTFTLSKAEGERQIGDM
                     VTGFAYTDIKQKARLTTKEIRSTRTSYGWGEVTEVRRDLGVFVDTGIPNKEIVVSLDV
                     LPEMKELWPKKGDKLYISLDVDKKDRIWGLPAEPEVFQKMASPAYNNMQNQHWPAIVY
                     RLKLTGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMIVTYLEANGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKRIKQ
                     DATGTELIG"
     gene            19489..19998
                     /gene="msrA"
                     /locus_tag="VHD02_000901"
     CDS             19489..19998
                     /gene="msrA"
                     /locus_tag="VHD02_000901"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEQ5497519.1"
                     /translation="MERAIFAGGCFWCMVQPFEEQAGILSVRSGYTGGHLPNPSYEQV
                     CAKTTGHTEAVEIIFDPKQIAYKDLVELYWAQTDPTDAFGQFEDRGDNYRPVIYYTTE
                     RQKEIAEQSKANLQASGRFDQPIVTTIEPAEPFYLAEDYHQGFYKKNPKRYAQSSAIR
                     HQFLEENWS"
     gene            19995..20210
                     /locus_tag="VHD02_000902"
     CDS             19995..20210
                     /locus_tag="VHD02_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEQ5497520.1"
                     /translation="MRKSFYSWLMTQRNPKSNEPVAILADLVFDDTTFPKHTNDFELI
                     SRYLEDQASFSFNLGQFDEIWEDYLAH"
     gene            20366..21535
                     /locus_tag="VHD02_000903"
     CDS             20366..21535
                     /locus_tag="VHD02_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEQ5497521.1"
                     /translation="MIITKKSLFVTSVALSLAPLATAQAQEWTPRSVTEIKSELVLVD
                     NVFTYTVKYGDTLSTIAEAMGIDVHVLGDINHIANIDLIFPDTILTANYNQHGQATTL
                     TVQAPASSPASVSHVPSSEPLPQASATSQSTVPMAPSATPSDVPTTPLASAKPDSFVT
                     ASSELTSSTNDVSTELSSESQKQPEVPQEAVPTPKAAETTEVEPKTDISEDPTSANRP
                     VPNESASEEVSSAAPAQAPAEKEETSAPAAQKAVADTTSVATSNGLSYAPNHAYNPMN
                     AGLQPQTAAFKEEVASAFGITSFSGYRPGDPGDHGKGLAIDFMVPENSALGDQVAQYA
                     IDHMAERGISYVIWKQRFYAPFASIYGPAYTWNPMPDRGSITENHYDHVHVSFNA"
     gene            21850..23622
                     /locus_tag="VHD02_000904"
     CDS             21850..23622
                     /locus_tag="VHD02_000904"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEQ5497522.1"
                     /translation="MYYTSGNYEAFATPRKPEGVDQKSAYIVGTGLAGLAAAVFLIRD
                     GHMAGERIHLFEELPLAGGSLDGIEKPHLGFVTRGGREMENHFECMWDMYRSIPSLEI
                     PGASYLDEFYWLDKDDPNSSNCRLIHKRGNRVDDDGQYTLGKQSKELIHLIMKTEESL
                     GDQTIEEFFSEDFFKSNFWVYWATMFAFEKWHSAVEMRRYAMRFIHHIDGLPDFTSLK
                     FNKYNQYDSMVKPIIAYLESHDVDIQFDTKVTDIQVEQTAGKKVAKTIHMTVSGEAKA
                     IELTPDDLVFVTNGSITESSTYGSHHEVAKPTKALGGSWNLWENLAAQSDDFGHPKVF
                     YQDLPAESWFVSATATIKHPAIEPYIERLTHRDLHDGKVNTGGIITITDSNWMMSFAI
                     HRQPHFKEQKENETTVWIYGLYSNSKGNYVHKKIEECTGQEITEEWLYHLGVPVDKIK
                     DLASQDYINTVPVYMPYITSYFMPRVKGDRPKVIPDGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYSFLNVERGIPEVFNSAYDIRELLKAFYYLNDKKAIKDMDLPIPAL
                     IEKIGHKKIKDTFIEELLKDANLI"
     gene            23781..24833
                     /locus_tag="VHD02_000905"
     CDS             23781..24833
                     /locus_tag="VHD02_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEQ5497523.1"
                     /translation="MQEYSIDITLTHPDDVLALFGSNERHLKLIEAHLGVIVHARTER
                     VQVIGDDEEAVELARLTIKALLVLVGRGMVVNTSDVVTALSMAESHQIDQFMALYEEE
                     IIKDNYGKAIRVKTLGQKTYVDSVKRHDVVFGVGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYHILGKEQTTRLMERDVIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDTSQIDLPRNVKS
                     GLIDATQKLQGIKQIDFVYFSAKDVVRHPVVADIIKAYETSSEETKDLLKPKASDVEA
                     DYQPGLTKYPVIGQEH"
     gene            24879..25454
                     /locus_tag="VHD02_000906"
     CDS             24879..25454
                     /locus_tag="VHD02_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEQ5497524.1"
                     /translation="MDDLTKAIMADEANLSYTERGIFPLYDAPQTARIIIVGQAPGIV
                     AQETKLYWNDRSGIRLRDWLGVDNDTFYHSGLFGIIPMDFYYPGKGKSGDLPPREGFA
                     AKWHPPLRALMPEVELTILVGRYAQDFYLGNKAYKTLTETVRHFEEYLPDYFPLVHPS
                     PRNQLWLAKNPWFEQDLLPILQKRVEAILTK"
     gene            25613..26110
                     /gene="ybeY"
                     /locus_tag="VHD02_000907"
     CDS             25613..26110
                     /gene="ybeY"
                     /locus_tag="VHD02_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEQ5497525.1"
                     /translation="MYIEMIDETGQVSQEIMEQTLDLLNFAAQKTGKEEKEMSVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPETPILFSQEDLAADPSLAEMMAEFDAYIGEL
                     FISIDKAREQSQEYGHSFEREMGFLAVHGFLHINGYDHYTLEEEKEMFTLQEEILTAY
                     GLTRQ"
     gene            26091..26498
                     /locus_tag="VHD02_000908"
     CDS             26091..26498
                     /locus_tag="VHD02_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEQ5497526.1"
                     /translation="MALHDNNTTKRKWKNRTITSSLEFALTGVFTAFKEERNLRSHLL
                     SACLACVAGLFFSISAIEWLFLLLAIFLVITLEIVNSAIENVVDLASDYHFSMLAKNA
                     KDMAAGAVLMISGYAVLTGLIIFIPKIWNIFVH"
     gene            26618..27514
                     /gene="era"
                     /locus_tag="VHD02_000909"
     CDS             26618..27514
                     /gene="era"
                     /locus_tag="VHD02_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEQ5497527.1"
                     /translation="MFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIM
                     GIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGDD
                     MIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALEGNNVPTL
                     IKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMKR
                     DEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWVK
                     VKKNWRDKKLDLADFGYNEKEY"
     gene            27534..28010
                     /locus_tag="VHD02_000910"
     CDS             27534..28010
                     /locus_tag="VHD02_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEQ5497528.1"
                     /translation="MPQDYISYIRSKVGHDKIILNFAGGILTNDDGKVLMQLRGDKKT
                     WAIPGGTMELGESSLETCKREFLEETGIEVEAVRLLNVYTHFEEVYPNGDAVQTIVFI
                     YELTAVSDMAIDNFHNEETLKLQFFSHEEIAELESVSAKHRLMLEEYFSDSFAMGH"
BASE COUNT         8760 a         5080 c         6265 g         8520 t
ORIGIN      
        1 gttctttctg gattatcact gtaataaata ctaaaccctc ggttaagcat ctcagctttt
       61 agcattagtt cccaagcatt acatataaaa aaactaaagc cttcaattcg atattttatt
      121 gtcggtttat tatatatttc gagccccata ataaaagctt cgacactttt taaaactaat
      181 ctttcactta aattttccat ttaattactc cgtcaaggtg acttttatta tttaagtggc
      241 ttaatgaatt ggttaaaatc tttttagttt agtaaaattg ctatttaagg ttagccacct
      301 ctcgtatggt cacagttgat gaattatgct atagtataga cataaaaaat agttcaaatc
      361 ccttcttgct tgttggtgga ttttgaactg aaagcctctc tgattgcagt tggtaggctt
      421 ttttgatgct ttttcgtact ttttatattt attacgctaa aaaatgttat acttgatgaa
      481 gaaaaaggag agtttagaac gttaagatac ctcacagcag attgatagag gatatggaag
      541 aaggttagct ttcccaagag atgccgtttg ggaatggtat aacaataact ggcagaagtt
      601 ataagtaaaa caagaacaaa ttaactcaga tgatttttag acgttcccac gcgtgtgcga
      661 atgcgtgtgc gaattgatgt tatcaataaa tcctttaata acgccttttt cgaacaggaa
      721 acaaatttat tatcccacgc gtgtgcgaat gtgtgggcga attaattgga gaaaaaatga
      781 gaaaatacga tatacaaaaa aggagaacaa tatgacattt gaagaaattc taccaggttt
      841 aaaagccaag aaaaaatatg tacgtacagg ttggggtggc gctgaaaatt atgtgcagtt
      901 gtttgatacc cttgaagtgg atgggaaagt actacaggcg acgccttatt ttctgattca
      961 tgttactggt gcaggtgaag gtttttctat gtgggcaccg acaccatgtg atgtgttagc
     1021 agaagattgg atcgaggtca atgactaaag ttgttttagt gacgggctgt gctagtggga
     1081 ttggttgtgc acaggcccgc tattttttga gacaagggta tcacgtatat ggtgtggata
     1141 aatcggataa accggactta aacggtaatt ttcactttat caaattggat ttatcctctg
     1201 agttatcccc actatttaaa gtggtcccca gtgttgatat tttgtgtaat actgcgggga
     1261 ttttagatgc ctacaagcca cttttagacg tcagtgatga agaagtggaa cacctatttg
     1321 atattaattt ttttgcgacg gtgaagttaa cacgtcatta tcttaggcgt atggtggaaa
     1381 agcagtcagg tgttatcatc aatatgtgct caatagcaag ttttatagcg ggtggcggtg
     1441 gtgttgccta tacgagttct aagcatgccc ttgcgggatt cacgcgccag ctggctttgg
     1501 attacgccaa agatcagatt cacatctttg gtatagcacc aggtgctgtc aaaaccgcta
     1561 tgacggctag cgactttgag cctggtggct tagcagactg ggtagctcgt gagacgccaa
     1621 ttggtcgttg gactgaacca gatgaagttg ctgaattaac cggatttctt gcttcaggaa
     1681 aagcgagatc aatgcaaggc gagattgtca aaattgacgg tggctggagt ttgaaatgat
     1741 cacatgcctt agcttgagct ggcgcagtaa gccctagagg tgtgatgagt caaacagtca
     1801 aaggagatga acaaatgaaa ttagcggttt tggggacagg tatgattgtt aaggaagttt
     1861 tgcctgtctt gcagaaaatt gatggtattg atttagttgc tatcttatca acagttagaa
     1921 gtttaaagac agctaaagac ctcgctaagg catatcacat gcctctagct acgagtaaat
     1981 atgaggctgt cttaggcaat gaggagattg acacggtcta tattggactg ccgaatcacc
     2041 tgcattttgc ctatgctaag gaggctcttc tagctggtaa acatgtcatt tgtgaaaagc
     2101 cctttaccat gacagcagga gaattagatg aactggtggt aatcgctaaa aaacgaaaac
     2161 tcattttgtt agaagcgatt acgaatcagt acctgagtaa catgaccttt atcaaagagc
     2221 atcttgacca acttggcgat atcaaaattg tagaatgtaa ctattcacaa tattcttcac
     2281 gctacgatgc cttcaaacga ggtgatatcg cgccagcttt taatcctaaa atgggtggtg
     2341 gagctttacg tgatttaaac atctacaaca ttcattttgt ggtaggatta tttggtcgcc
     2401 ctaaaacggt tcaatattta gccaatgtcg aaaaaggcat tgacacatca ggcatgcttg
     2461 tcatggacta tgagcagttt aaagtggtat gtatcggggc caaagactgc actgcagaaa
     2521 tcaaatcaac catccaaggt aataaagggt cactggcagt gcttggagca acaaataccc
     2581 ttcctcaggt tcagttaagt ctacacggtc aaaaaccaca agtgattaac cacaacaagc
     2641 atgaccacca gatgtatgag gagtttgtcg cttttaaaga catgattgac caaaaagact
     2701 ttaagaaagt gaatcaggct ttggagcata gccgagctgt catggctgtg ttggagcgag
     2761 ctgttcattc gtgattgtaa cctagtaacc attgcctttt gtgcaggtgc tatggtaatg
     2821 gttgaaagac gtgttttcac gagggttaac cccttcgtgc ttttttggca aaaataactt
     2881 gttcaggatt tgggctttct ttgtgagatg agagatgcct gacgaattga tttgattaaa
     2941 ataatgataa agccaaacga tttaagaaat gaccgttcgg aaattctgcc ccaaaaggtc
     3001 tctagaagca gtaaaatcga atttgtaagc gttgacactg ttgcataact ctgttacact
     3061 aagagaatgt catgacaaaa attacagtgt aggagtttaa ttgtttatgg aagaggatta
     3121 taacaaaaga gaacctgaga agtttacgca atttctgcgc cgtcaaaagg tcgtcttttt
     3181 tgtcgcattt tttggttatg tgtgtgccta cttggtacgc aacaatttca aactaatgtc
     3241 aaacactatt atggtgcaaa atggttggga caaggctcaa attgcgacct tgttatcgtg
     3301 tttgaccgtt tcttatggtt tagcaaaatt ctatatgggg gccttagggg atcgtgttag
     3361 cttgagaaag ctcttttcga tcagcttggg tgccagcgct ttgatttgta ttttaattgg
     3421 ttttttcaat agttcaatgg tggtattagg gatactgcta gtcttgtgtg gtgtcgtaca
     3481 aggtgccttg gcacctgcct cacaagccat gattgccaat tattttccaa ataaaacacg
     3541 cggcggagcc atcgctggat ggaacatttc tcaaaacatg ggatcagccc tcttgccatt
     3601 aaccatcgcc ttgctcacca gtatgggctt ggtggtacca gctaatggca atattttact
     3661 tgccttttta atccctggcg ttttagtgtt cttgtttgct ttgtgttgct ggaagcttgg
     3721 cggtgataac cctgagtctg agggacttga ctctcttcga acgatgtatg gtgatgcggg
     3781 cgagtcagct gttgccagtg aagaagaaaa gcataacctg tcttattggc aactcatctg
     3841 gaaatacgtt ttttgtaacc cgtcgctctt acttgttgct gctgtcaatg tggcccttta
     3901 ttttgttcgt tttgggattg aagactggat gccgatctac ttgtcacaag tagccaatat
     3961 gtcagaggct catatccatt ttgcgatttc aatattagag tgggtagcta ttccaggctc
     4021 gctggtattt gcgtggttag cggttcgtta tcctaataaa atggccaaga ttggagctat
     4081 tggacttttt gtgttagcgg ctattgtctt tgtctatgaa cgcttgactg ccacaggtgc
     4141 tccaaactat ttcttgttgc ttgttattgc aggtatttta gggtcattga tttatggccc
     4201 acagttgatc gtgaatattt taacaatcaa ctttgttcct ttaaatgttg caggaacagc
     4261 gattggtttt gtaggagtaa cagcttatct catcggcaat atgggagcaa actggctgat
     4321 gccgattttg gcagatggtt ttggctggtt ttggtcatat attgtcgttg cagccttatc
     4381 tgctttttca gcggttggtt atttgatttt agccaaacgt gaggaagaaa tcatcaaaga
     4441 ttagctaggc aaatgcctaa gaaagacaga cttattgcaa taagtcctgt cttttttagt
     4501 catcacaagt cgcaagtgac actataaaag acaagaaaat gctaggatgt ttcaggaaaa
     4561 aaagagaaaa tatgctataa tgagaaggta tttaaattat atagtaagga aaaacaaatg
     4621 acaaattatg ccattatttt agcagcgggc aaagggactc gcatgacttc tgatctccca
     4681 aaagttcttc ataaagtatc tggactaacc atgcttgagc atgtttttcg tagtgtaaaa
     4741 gcgattagtc ctgaaaaagc cgtgacggtt attggtcaca aatcagaaaa ggtacgtgct
     4801 gtcttagcag atcaatcagc ctttgtccat caaacagagc agttaggaac tggccatgcc
     4861 gtgatgatgg cagaaacaca actagaagga ctagaagggc ataccttggt gattgcagga
     4921 gatactccct tgatcactgg agaaagcctg aaaagtctga tcgattttca tgtgaatcat
     4981 aaaaatgtcg ccactatttt aacagctacg gctcaagatc catttggtta tggtcgtatt
     5041 gttcgcaaca aagatggcga agtgatcaag attgttgagc aaaaagacgc caatgagtat
     5101 gagcaacaat taaaagaaat caatacagga acctatgtgt ttgataacaa gcgtctcttt
     5161 gaagcgctca aatgcatcac taccaacaat gctcaaggag agtattacct gactgatgtt
     5221 gtggctattt ttagagcaaa caaagaaaag gtaggagcct atatcctgag agacttcaat
     5281 gaaagtcttg gtgttaatga tcgtgtggcc ttagcaacag ctgaaacagt gatgcgccaa
     5341 cgcatcaccc aaaaacatat ggtcaatggg gtgactttcc aaaatcctga aaccgtttat
     5401 attgaaagcg atgttgagat tgcaccggat gttttaattg aaggaaatgt gaccttaaaa
     5461 ggacgtacac atatcggctc aggaactgtc ttgacgaatg gtacttatat tgtggattct
     5521 gaaattggtc aaggaagtat catcaccaac tcaatgatcg aatcatctgt tttagctgca
     5581 ggagttacgg ttggccctta cgcccacctt cgaccaggaa cgaccttagg tagggaagtc
     5641 catatcggta actttgttga ggtcaagggt tctcacattg gtgaaaaaac aaaggctggg
     5701 catctgactt atattggaaa cgctcaggtt gggtcttcag tcaatgttgg ggctggaacc
     5761 atcactgtca actacgacgg ccaaaacaaa tatgaaacag tcattggaga tcacgctttt
     5821 attgggagca actcgactct cattgcacct ttggaagttg gagataatgc tttaactgca
     5881 gctggctcaa cgatttctaa gacagtacca gctgatagca ttgttattgg tcgtagccgc
     5941 caagtcacaa aagaaggtta tgccaagcgc ctagcgcatc acccaagtcg aagcaaataa
     6001 ttcaagatct atttattagt aaggaggcca ggcatgaaat ttgaagaaaa aacgcttaag
     6061 agacaaacag tttttgatgg tcatattttt aaagttgttg tggatgatgt tgaacttccc
     6121 aacaatctgg ggcaatccaa gcgagaatta atttttcatc gtggtgccgt tgctgttttg
     6181 gctattactc ctgaacgtaa gattgtcctt gtcaaacaat accgtaaggc tattgaaagt
     6241 gtctcatacg agatcccagc gggtaagcta gagattggtg aggaaggctc aaagctaaaa
     6301 gctgctgcac gtgagctgga agaagaaaca gcctatacgg ggacattgac gtttttgtat
     6361 gaattttata cagcgattgg tttttgcaac gaaaaaatca cacttttctt agcgacagac
     6421 ctaacacaag ttgccaatcc caaacctcaa gacgacgatg aagttatcga ggtcttagag
     6481 ttgacatatc aagagtgcat ggacttagtc gctcaaggta aacttgctga cgctaaaacg
     6541 ttgattgctt tgcaatacta tgccttacat tttggaggtg agcagtgatg ggaaggccat
     6601 tgttgaccga tgatatcatc gaaaaagcga gacgaatgga aacatttgag ccagatgatg
     6661 ctgtagactt tgacacaaaa gtaatgacgc taccagaaaa agatgaaaaa gcacgcattt
     6721 ataaaagccg tcggattgaa aatgccaaac gcagccaatt gcaatctaaa ttaaatgtga
     6781 ttttgatagc tgttatgctg ttaattgcca tcttggtgta tgctatcttt tatctttaaa
     6841 aaaacgtaag gaaatgatat gaaaatagga attattgctg ctatggagga ggagttgtcc
     6901 ctgctcttag caaatctttt ggatgctcaa gaacatcagg tgctttcaaa aacttactat
     6961 acgggtcgtt ttggcaagca tgagctcatt ttggtacagt caggtgttgg aaaagtgatg
     7021 tctgctatga cagttgcgat cttggctgag catttcaaag cccaagctat tatcaataca
     7081 ggatcagcag gggcagtcgc ttcacattta gcgatcggag atgtggtggt tgcagatcgg
     7141 ttagtgtatc atgatgtgga tgctactgca tttggttatg cttatggtca aatggctggt
     7201 caaccattat actatgattg tgatccacag tttgtggcta tcttcaaaca ggtcctaaaa
     7261 cacgaaaaga caaatggcca agttggtttg attgcaacag gagacagttt tgttgctggt
     7321 caggacaaga ttgaccagat caaaacagct tttagcgacg tgttagcagt agaaatggaa
     7381 ggcgcagcta ttgctcaagc agcacatact gcaggtaaac ctttcatcgt tgttcgtgcg
     7441 atgagtgata ccgcagcgca tgatgccaat attacttttg atcaatttat tatagaagct
     7501 ggcaagcgtt cggcccagat attaatgaca tttttagaaa acttacctgt ttaactcacc
     7561 ttatcgtgag acaattagct ggataatctc tattttagga gtctggtcac ctcttgtttt
     7621 gaaacctaaa aataagaatt gcaatcactt gaccaaacgc tagcatttga ctggtccaaa
     7681 atcacacctg caatatagta aagacaacac caaacttcaa ttgagaaata tccttaacgg
     7741 ctaccaaagt gacctaggga gacactactc tagctattat tactacaacc taagaaccgt
     7801 tatgggacta tcaagtgagc aagagattga agaacactat gaaaagctta agaacaagtt
     7861 acatgatatg tacaatcatt attaagtcaa aggcttaaaa atggcgacta gcccctcaaa
     7921 aagaggccac actcatttaa atttaaatga gtgtggcctc tttttgagga ggcgcaaggg
     7981 gttaaagggc tgtgacataa agttgcttag caatattttc agggatgaca agttccttgt
     8041 cgccataaca aatcgtatag gtttttgcaa aggtatcaat ttgagttaag gttagctcgg
     8101 tattgatact caggtgatgt gcttctagat actggattaa atcaaagtgg tcgtggattc
     8161 tgcttaggcg aaaccgtccc aactccgtga tggtgttgag agttgtagtg ttcatttcga
     8221 caagaggctg accatagcga ggaatagtcc ctccgtgtgg gcagaaatct ggaaaatcaa
     8281 ggatcttgtc taaacggtcg ataaaggtgt ctgagacagt gtgctctaag acttcagctt
     8341 cttggtgcac ttcttgggta ttatagccca gctggtgaat caaaaatacc tcaatcaagc
     8401 ggtgttttcg gtagaggtta gcaacaagag catagccttt gtcttttagc aaatagcctt
     8461 ttgctttatc tttgacaatc cagccttgtg aaatcatttt tttaatcatc tctgaaacgg
     8521 caggtgccga aacgtgcatc ttttctgcaa ccattttatt tgtaatcttg ggttcttgtt
     8581 ctccaatttc atagatacat ttcaagtaat cttctttatt aggcgtcata tcgtttggcc
     8641 tttctcttat ttataatgat tattatatca gaatttttag gaaaaagctt gacaaaaagc
     8701 ctaaaagagg atatccttat agcataatta actaaactta attaaataaa taatatatcc
     8761 taaggagaga aatgatggga aagagaatga gcctcatttt gggtgctttt ttatctgttt
     8821 ttcttttagt agcttgttcg tcaactggca ctaaaactgc taagagtgat aaattaaaag
     8881 tcgtggcaac caattcaatt attgccgaca tgacaaaagc tattgctggt gataaaatcg
     8941 atctgcacag cattgtgcca atcggtcaag accctcatga gtacggacca ttaccagaag
     9001 atgttgaaaa aacaagtaat gctgatgtga ttttctataa tggtatcaat ctagaagatg
     9061 gtgggcaagc ttggttcacc aaactagtga aaaatgctca aaaaacgaaa aacaaagatt
     9121 actttgccgt gtctgatggc attgatgtga tttacttgga aggtgcaagc gaaaaaggaa
     9181 aagaagatcc acatgcttgg ttaaatctcg aaaacggaat catttattca aaaaacattg
     9241 ccaaacaatt gattgcaaag gatcctaaaa acaaagaaac ttatgaaaag aacctaaaag
     9301 cttatgtggc taaattggaa aaactagaca aagaagccaa atcaaaattt gatgctattg
     9361 cagaaaataa aaaattgatt gtgactagtg aaggctgctt caagtacttt tcaaaagctt
     9421 acggtgtccc atctgcttat atctgggaaa ttaacaccga agaagaagga acaccagatc
     9481 aaatttcatc attgattgaa aaactaaaag tcatcaagcc atctgcgctt tttgtagagt
     9541 caagtgtcga tagacgccct atggaaactg tttctaaaga tagtggtatt cctatttatt
     9601 ctgagatctt tacagattca attgctaaaa aaggtaaacc tggcgatagt tattatgcta
     9661 tgatgaaatg gaaccttgac aaaatttcgg aaggtctagc aaaataagat aacaataaag
     9721 cacagcgatt ttagtgttgt gctttgtttt atttatgtaa aggagacaaa atgattacaa
     9781 ctaacaatct ttgtgtgacc tacgatggca acagtaatgc tttagaagcg attaatgtga
     9841 caattgaagg tccttctatc gttggtatta ttggtcctaa tggagctgga aaatcaacct
     9901 ttatgaaagc tatcttaaat cttattgatt atcaaggtca cgtaacggtt gatggcaaag
     9961 atggtcgtaa gcttgggcat acagtagctt acgtagaaca acgaagcatg attgactata
    10021 attttcctat tactgttaaa gaatgtgtgg cgctagggac ttatagtaaa ttaggccttt
    10081 ttcgccgagt aggaaaaaaa caatttgagc aggttgataa ggttctcaaa caagttggtt
    10141 tagaagactt tggccatcga ccgataaaat ccttatctgg tggtcagttt cagcggatgt
    10201 tggttgcgcg ttgcttgatt caagaatcag actatatttt cttagatgaa ccgtttgttg
    10261 gcattgactc ggtcagcgaa aaaatcattg tagatctttt gaaagagctc aaaatggctg
    10321 gcaaaacgat tttaattgta catcatgatt tgagcaaagt agagcattat tttgacaaac
    10381 tcatgatttt aaataaacat ttggtggctt atggcaacgt ttgtgaggta tttactgttg
    10441 ataccttgtc aaaagcttat ggcaatcatt tgattttagg aaaggagatg gtgtaaatgt
    10501 ctatgaaatt ttttgagggc ttgatgtcat accacttttt acaaaatgca ctgataacgg
    10561 ctgtagtcat tggtatcgtc tcaggtgctg tagggtgttt tattatcctt aggtcaatgt
    10621 ctcttatggg tgatgccatc tcacacgctg ttttaccagg ggttgctttg tcatttattt
    10681 taggagtcaa tttttttatt ggagcgatta tttttgggtt attagcttct gttattatta
    10741 cttatatcaa ggaaaactct gtcattaaag gagatacggc tattggtatc acctttagct
    10801 cttttttggc actgggagtc atcttgatag gggtagccaa tagttcgacg gacttatttc
    10861 atattttgtt tgggaatatt ttagctgtgc aagatagcga taagtggatt actattggtg
    10921 tttcgatttt tgttttagtg gttattagtc tctttttcaa agaactatta ttaacatcat
    10981 ttgatcctat cttagccaaa tcaatgggtg tgaaggtcaa tgcgtatcat tatttgttga
    11041 tggtcctatt aactttagtg gctgttacgg cgatgcaaag cgtgggtact atcttgattg
    11101 ttgccttatt gattacgcca gcagcgacag cttatttata tgctaatagc ttaaaagtaa
    11161 tgttagtgat gtcatcttta ctaggcgctt tagcatcggt tttagggctt tatttgggct
    11221 atacctttaa tgttgccgca gggtcaagta tcgtactgac ttctgcgatg atgtttttga
    11281 tcagtttctt tgtttcaccc aagcaaggct acctcaaaag atggatgcaa aaaaaagaaa
    11341 aaacacctta aacttttcgc tttaaacacc ttagtggttt gcaaatagta gcaaataaca
    11401 tcaaagggtg ttttttatat gtcatcaaaa gaccataaaa aaccttatca aaccaaggta
    11461 tttagcctat tatctgataa ggttttttta agcatgattt agtttttaaa gcgataatct
    11521 ttgacaatgt caattgaggt aatcgtaatg tcttgttctg gtttatcatt ttggttgata
    11581 gaagtcgcgg caattttatc gacaacatcc ataccatcaa tgacttgacc aaatacagta
    11641 taaccgccat ctaagcttgg attgccgcca tgctcatagg cagagatgat aggttttggg
    11701 tagttggtac ttgataatcc cttgctttga tttttcttgt tttggttgat ataaaattgg
    11761 ctaccgttcg tattagcgcc agcatttgcc atggcaagag caccgcgaat atgatataaa
    11821 aatggagaga tttcgttgac aaagccattg ccagcatctt ttttaggatc cttgcccttc
    11881 caaatcgatt caccacctgt gccatctcct ttggggtcac ctgattgaat cataaagtcg
    11941 ttgatcacac gatggaaagt aaggttatca tagtagcctt ttttagcatg ggtgaggaag
    12001 ttctcaacag ctaagggagc atattttggg aataacttaa gggtaatgtc cccttgactt
    12061 gtcctcatta cgaccttagc ttcttctttg ccgacttctt tggttagttg tggaaattgg
    12121 ctagcatcag ctttgagagc tttttgaatg ctttcttcat aggctttaga agctgcttcg
    12181 ctttcttctt tggcagtttt ttcatcaatg tatttatcgc cttttatagc gcggtcaaca
    12241 gactcacaag cactcaaact aagtaaacta atggctacta aactgagaga taataatttt
    12301 ttcatgtcta ctcctaagaa tgattttctt tattttatca taaaatgcaa cagaaaatag
    12361 tagtaaagag ttaaaaacgc ctatttttgt gttttatgat ctaaccttgg ttaaaaggct
    12421 gctacgactg ttattatagc acaagatgct atagaaaaac ttaaaacacc ttggtttttt
    12481 agagctattt cctaatagtt aaaaaatggt ataatagtgt tatggttaaa agaaatcaaa
    12541 gaagaaaaag tgcgcctaag aagcgtctga caaaagcaga agtggaaaag caacgtgcca
    12601 ttaagaggat gattttatct gttttaatgg ctttgttgct tatttttgcg atgcttcgct
    12661 tgggcgtctt tggcgtgaca acttataata tgattcgttt tttggtgggt agtttagcct
    12721 atccgtttat gtttgcctgg ttaatttact tattttgttt taaatggtta cggcaaaaag
    12781 atggaatgat tgcgggagtt gttattgcct ttttgggatt gctagttgag tggcacgcct
    12841 tcttatttgc gatgcctagg atgtttgatc aagatatttt tctaagcact gctcggctaa
    12901 ttacaagaga tttactagcc ctgcgcgtga ctgaatttgt tggtggagga atgcttggtg
    12961 cattacttta taaacccata gcctttttat tttcgaatat cggctcttac tttattggtt
    13021 ttttgtttat cttattagga ttatttctaa tgacgccttg ggatatctat gatgtgagtc
    13081 actttgtgaa agaggctgtt gataaactag cagtggctta tcaggaaaat aaagaaaagc
    13141 ggttcatcaa acgagaagag caccgcttac aagctgaaaa agaggcttta gagaaacaag
    13201 cacaagagga agaaaaacgt ctagcagaat tgacggttga tcctgagacg ggagagattg
    13261 ttgaggatag tcagtctcaa gtcagctatg atttggctga ggatatgaca aaagaaccag
    13321 agattcttgc ttatgatagt cacctcaaag acgatgaggc aagcttattt gaccaagaag
    13381 acttggctta cgctcacgag aaaattgggg cttatgactc tttatctgct ttagcatctt
    13441 ctgaagatga gatggatatg gatgaacctg ttgaagttga tttcacacca aaaacccatc
    13501 ttctttataa acttccaacg atagatttat ttgcaccaga taagcccaaa aaccagtcca
    13561 aagagaaaaa cttagtccgc aaaaatatca aggtgcttga agataccttc caaagttttg
    13621 ggattgatgt caaggtagaa cgagcagaaa tcgggccttc cgtcaccaaa tatgaaatca
    13681 agccagctgt cggggtccga gttaatcgta tttcaaatct tgctgatgat ttggcacttg
    13741 ctctggctgc taaagatgtt cgtatagaag caccaattcc tggaaaatcc ttaataggta
    13801 ttgaagttcc taactcagaa attgcaacgg tttctttccg cgaactttgg gaacaatctg
    13861 atgccaatcc tgaaaacctt ttagaagtac cactaggtaa agctgttaat ggtaacgctc
    13921 gcagttttaa cttagctaga atgccgcatc ttttggtagc tggttcaact ggttcaggta
    13981 aatcggttgc tgttaatggc attatttcaa gtattctgat gaaggctaga ccagaccaag
    14041 tgaaattttt gatggtcgac cctaagatgg ttgagttgtc tgtttataat gatattcccc
    14101 atttgctgat tccagtagtg accaatcctc gtaaagccag caaggcgttg caaaaagtgg
    14161 ttgatgagat ggaaaatcgc tatgaactct ttagtaaagt cggggttcga aatattgctg
    14221 gttacaatgc aaaagttgag gattacaata ggcaatctga gcaaaaacaa atgcctttac
    14281 cattgattgt ggttattgtg gatgagttag ctgatttgat gatggtggct agtaaagaag
    14341 tggaagacgc tattatccgt ctggggcaaa aggcgcgtgc ggcaggtatt cacatgattc
    14401 ttgcaaccca aagaccatct gtggatgtta tttctggact gattaaggcc aatgtgccat
    14461 cccgtattgc atttgccgta tcttcaggaa cagatagtcg aacgattctg gatgaaaatg
    14521 gcgctgaaaa gctcttaggg cgtggagata tgctctttaa gccgattgat gaaaatcacc
    14581 ctgttcgtct gcaagggtct ttcatctcag atgatgatgt ggaacgaatt gtaaacttta
    14641 tcaaagacca agctgaagcg gattatgatg acgcctttga tcctggtgaa gttagcgaca
    14701 atgatcctgg tttttctgga aatggtggag ctgctgaagg tgatcctctt tttgaagaag
    14761 caaaagcctt ggtcttagaa acacaaaaag ctagtgcctc aatgattcag aggcgtttgt
    14821 cggttggatt taatcgtgcc acgcgcctga tggatgaatt ggaagaagca ggtgttatcg
    14881 gtccagcaga aggaaccaaa cctcgtaagg tattgcagac aaactaaaag aacctgacgt
    14941 ctcatcatga gatgtcaggt tcttttttgg tgatgtcagg ccagttgatg agatgcctat
    15001 gctaatgtaa aaataaagat aactagaata aggtagaaaa taacggtgac taaaaagcca
    15061 actcgccaaa atagcttcat aaagcgatgg taagaaaacg actgagttcg gatgactagc
    15121 agtagtgaaa tgacgatggc tagcagcgac atgataataa ggtaataagg cagtaaatta
    15181 tgggtgaaaa attttcctga gacaagcaca atttctatcg caaaaagtaa aagtgcgaca
    15241 tctggaaatt taaatccctt tttattaagt ttaaatagag gggttacaat catggcaaaa
    15301 agaggcgtta caatcaagaa agctaaggca gttaatttat agatcatcat aggtctattt
    15361 tatactcaaa tcccttactt gtaaactgat acttacctgt tgtgatagaa actgatcttt
    15421 gagagggaaa gagcctactt caatttaaac attacaaaag gcatcagcta ttttaacaaa
    15481 aagacttgat ttattttggc aataagctat aatggtaagg tatgcaatat tgcatatctg
    15541 tgggaggtaa aaatctcaat taccgccaaa accacaacag gaggattttt aaaatggcta
    15601 aaaaagtcga aaaacttgta aaacttcaaa tccctgctgg taaagctaca ccagctccac
    15661 cagttggacc agctcttggt caagcaggta tcaacatcat gggcttcact aaagaattta
    15721 acgctcgtac agctgatcaa gctggtatga tcatcccagt tgttatctca gtttatgaag
    15781 acaaatcatt tgatttcatc actaaaacac caccagctgc tgttcttttg aaaaaagctg
    15841 caggtgttga aaaaggatca ggtacaccta acactactaa ggttgcgaca gttactcgtg
    15901 cacaagtaca agaaattgct gaaactaaga tgccagattt gaacgctgca aacattgaag
    15961 ctgcaatgcg tatgatcgaa ggtactgctc gttctatggg attcactgtt actgactagt
    16021 cagtcaagaa cccctattac ccgcacgact tcatcataag agatgagtga cgtgggagat
    16081 tttaaatcaa atcgatatga ccacattaca aggagaattt taaaatggct aaaaaaagca
    16141 aacaaatgcg tgctgcactt gaaaaagtag atagcacaaa agcgtacagt gtagaagaag
    16201 ctgtagcatt agtaaaagaa acgaacttcg caaaatttga tgcgtctgtt gaagttgctt
    16261 acaacttgaa cattgacgtt cgtaaagcag accaacaaat ccgtggcgca atggtattgc
    16321 caaacggaac tggtaaaaca caacgtgttc ttgtttttgc acgtggtgca aaagctgaag
    16381 aagcaaaagc agctggtgca gacttcgtag gtgaagatga ccttgttgca aaaatcaatg
    16441 gtggatggct tgactttgac gttgttatcg caacgccaga catgatggct atcgtaggtc
    16501 gccttggacg tgtccttgga cctcgtaact tgatgccaaa ccctaaaact ggtacagtaa
    16561 cgatggatgt tgctaaagcc gttgaagagt ctaaaggtgg taaaatcact taccgtgctg
    16621 acaaagcagg taatgttcaa gctcttattg gtaaagtttc atttgatgct gacaaattgg
    16681 ttgaaaactt caaagccttc cacgatgtaa tggctaaagc taaacctgca acagctaaag
    16741 gaacttacat ggcaaacgtc tcaatcacat caacacaagg tgttggtatc aaggttgatc
    16801 ctaactcact ttaagaaaaa aattagggca acgcctaatc atagcaaaaa gacaaatttc
    16861 tgaagacaaa tcttcactag aggtttgtct tttttatggt ttaaaatgga atgcaaacaa
    16921 aaaagtgtag gcgataatac catgaaagga gttgattctc ttaatgagat gtggtgttaa
    16981 cacatcatga gctcacaaaa aatgataaag gaagtatcgg tggtgttaaa aaagggattt
    17041 taattgacat ttattagcct ctaaaatgtc ctttccctat tcataattga gggtttgtgg
    17101 taaaatagta aggacaaact aaggagaaca tagtagtgga acctaaatat caacgtatat
    17161 taattaagtt atctggtgaa gcattagcag gtgaaaaagg agttggcatt gacattccaa
    17221 ctgttcaggc tattgcaaaa gaaattgccg aagttcatgt gtcgggagta cagatcgctc
    17281 ttgttattgg tggaggcaat ctctggcgtg gagagcctgc agcagacgct ggaatggacc
    17341 gtgttcaggc tgattacact ggcatgcttg gaacggtgat gaatgcccta gtcatggctg
    17401 atagtcttca acattatggt gttgataccc gtgtgcaaac agctattcct atgcaaaatg
    17461 tagcagaacc ttatattcgc ggacgtgcct tgcgccatct agaaaaaaat cggattgttg
    17521 tttttggagc tggtattggc tcgccttatt tttcaacaga caccaccgcg gcactgcgtg
    17581 ctgctgagat tgaagcagat gctattttaa tggcaaaaaa tggtgtggat ggggtctata
    17641 atgctgatcc taaaaaagat gctaatgcgg ttaaatttga tgaattaaca catggtgaag
    17701 tgatcaaacg tggtctcaaa atcatggatg caacggcatc gaccttatcg atggataacg
    17761 atattgattt ggtggttttc aatatgaatg aagctggcaa tattcaacgt gttgtctttg
    17821 gagaacatat tggaactact gtatcaaata aagtttgtga ctaaaagtta gaaaagaaag
    17881 agagtaatta tcatggcaaa tgcaattatt gaaactgcaa aagaacgttt tgcacaatcc
    17941 catcagtctt tatcacgtga atatgccagc attcgtgcgg gtcgtgccaa cgctagtctt
    18001 ttagaccgta tccaagttga ttattacggg gcaccgacac cattaaatca attggcttca
    18061 atcactgtac cagaagcgcg tgtcttattg atctcgcctt ttgataaatc ttctatcaaa
    18121 gatatcgagc gcgctcttaa cgcatcagat ttaggcatta cacctgctaa cgatggctct
    18181 gtcattcgtt tggttattcc agctttgacc gaagaaacac gtaaagaatt agccaaagaa
    18241 gtgaaaaaag ttggtgaaaa tgctaaaatt gcaatccgta atatccgccg tgatgctatg
    18301 gatgatgcta aaaagcaaga aaaagcaaaa gaaatcactg aagatgagtt aaaaacactt
    18361 gagaaagata tccaaaaagc aaccgatgat gctatcaaag aaattgaccg catgacggct
    18421 gaaaaagaaa aagaattgct ctcagtgtaa caggatttct acgaaaaata gacgactata
    18481 gacacaaaaa gataggtgac tatttcagaa gcgtttggtt ttgatttgtc cctatttttt
    18541 taaagaaaga aaaaagatat gaatgattta ctagcaacag tcattactgg gcttattaaa
    18601 gaagaaaacg ctaatgacta ttttatccaa aaagaggggt ttacctttac cttgtctaaa
    18661 gcagaaggag aacgccagat tggtgatatg gtcacaggct ttgcctatac agacataaag
    18721 caaaaggcac gtctcacaac caaagagatc cgttcaacac ggacaagtta tggctggggg
    18781 gaagtgacag aggttcgtcg tgacctaggc gtttttgtcg atacaggaat tccaaataaa
    18841 gaaattgtgg tttctttgga cgtgttgcct gaaatgaaag aactatggcc taaaaaaggg
    18901 gataaacttt acattagttt ggatgtggat aaaaaagacc gtatttgggg acttccagca
    18961 gagcctgagg tctttcaaaa gatggctagc cctgcataca ataatatgca aaatcaacat
    19021 tggccagcaa ttgtttaccg cctaaaatta acaggaacgt ttgtttacct gcctgaaaac
    19081 aacatgttag gctttattca cccaagtgaa cgctacgcag aaccgcgctt aggtcaggtt
    19141 ttagatgccc gtgtgattgg gtttagagag gtcgatcgta ccctaaactt gtcgttgaaa
    19201 ccacgttctt ttgagatgtt ggagaatgac gctcagatga ttgtcaccta tcttgaagcc
    19261 aatggtggtt tcatgacctt aaatgacaaa tcaagtccag aagagattaa agcaaccttt
    19321 ggtatttcaa aaggccagtt taaaaaagcc ttgggtggat tgatgaaagc taaacgcatc
    19381 aaacaagacg ccacaggaac tgaattaata ggataaaaat gctttgccac tggcaaagtt
    19441 ttttgttaca atgaagacca ttaagtgaag aaaaagggga tatagagaat ggaaagagcc
    19501 atttttgcag gaggttgttt ctggtgtatg gtgcaacctt ttgaggagca agcaggtatt
    19561 ttgtcagtca gaagtggcta cacaggtggg catctcccaa atcctagcta tgaacaagtt
    19621 tgtgccaaaa caacaggaca tacagaagct gttgaaatta tttttgaccc taagcaaatc
    19681 gcttataaag atttggtaga gctctattgg gcgcagacgg atccaacaga cgcctttggg
    19741 caatttgaag atcgtgggga caactaccgt cctgtcattt actacacaac ggaaagacaa
    19801 aaagaaattg cagagcaatc aaaagcaaac ttgcaagctt caggacgttt tgaccaaccc
    19861 attgtcacca ctattgaacc agcagagcca ttttatctcg cagaggatta tcaccaagga
    19921 ttttataaaa agaatcccaa gcggtatgca caaagcagtg ctattcgtca tcagttttta
    19981 gaggagaact ggtcatgaga aaatcatttt acagctggct gatgacacaa cgtaacccta
    20041 aatcaaatga acctgtggcg attttagcag atttggtgtt tgatgacacc acttttccaa
    20101 agcataccaa tgattttgaa ctcattagtc ggtatctaga agatcaagct agtttttcct
    20161 ttaatttagg tcaatttgat gaaatctggg aagattatct agcacattaa tacgtggata
    20221 aaaaagagtc atatcgactc ttttgaaggg ttatcacgtt attattacaa aatgacatca
    20281 ctttctttca aaaaaatgac aagctacttt ttttcttaaa aatatgctat gatatagctt
    20341 agaaaaaaat aaaaaggaag cagagatgat tattactaaa aagagcttat ttgtgacaag
    20401 tgtcgctttg tcgttagcac ctttggcgac agcgcaggca caagagtgga caccacgatc
    20461 agttacagaa atcaagtctg aactcgtcct agttgataat gtttttactt atactgtaaa
    20521 atacggtgac actttaagca caattgctga agcaatggga attgatgtgc atgtcttagg
    20581 agatattaat catattgcta atattgactt aatttttcca gacacgatcc taacagcaaa
    20641 ctacaaccaa cacggtcagg caacgacttt gacggttcaa gcgcctgctt ctagtccagc
    20701 tagcgttagt catgtaccta gcagtgagcc attaccccaa gcatctgcca cctctcaatc
    20761 gactgttcct atggcaccat ctgcgacacc atctgatgtc ccaacgacac cattagcatc
    20821 tgcaaagcca gatagttttg tgacagcgtc atctgagctc acatcatcaa cgaatgatgt
    20881 ttcgactgag ttgtctagcg aatcacaaaa gcagccagaa gtaccacaag aagcagttcc
    20941 aactcctaaa gcagctgaaa cgactgaagt cgaacctaag acagacatct cagaagaccc
    21001 aacttcagct aataggcctg tacctaacga gagtgcttca gaagaagttt cttctgcggc
    21061 cccagcacaa gccccagcag aaaaagaaga aacctctgcg ccagcagcac aaaaagctgt
    21121 agctgacacc acaagtgttg caacctcaaa cggcctttct tacgctccaa accatgccta
    21181 caatccaatg aatgcagggc ttcaaccaca aacagcagcc ttcaaagaag aagtggcttc
    21241 tgcctttggt attacgtcat ttagtggtta ccgtccaggt gatccaggag atcatggtaa
    21301 aggtttggcc attgatttta tggtgcctga aaattctgct cttggtgatc aagttgctca
    21361 atatgccatt gaccatatgg cagagcgtgg tatttcatac gttatttgga aacagcgatt
    21421 ctatgcgcca tttgcaagta tttacggacc agcttacaca tggaacccca tgccagatcg
    21481 cggcagtatt acagaaaacc attatgatca tgttcatgtc tcctttaatg cttaattcat
    21541 gctatcaaaa aaaactcacg tttaggcgtg agttttttga caattctgac agagtaagcc
    21601 ctataagagg ctgagatacg cttttaatac ctgttgtttt tctcaccctt tagacagttt
    21661 taacgttatg tctaaaagag atggttttta ttagcgcaaa tatgggttta ggggtaaaat
    21721 ataggtaatt aacctgatat gatcatgata aaggtagaag aatacctatt atttcgtgac
    21781 aactgagcaa tctgtcttac ctttggagtg atgatatcac tttgcttgac tattcaagga
    21841 ggtcttgaaa tgtattatac tagtggtaat tacgaagctt ttgcaacacc tcgaaaacct
    21901 gaaggggtag atcagaaatc agcttatatt gttggcactg gtttagctgg tttagcagca
    21961 gctgttttcc ttattcgcga tgggcatatg gctggggaac gcattcatct gtttgaggaa
    22021 ttgcctttag caggtggttc tttagatggt attgaaaagc ctcatcttgg ttttgtgacc
    22081 cgtggtggtc gtgagatgga aaatcatttt gagtgtatgt gggacatgta tcgctctatt
    22141 ccctcactgg aaattcccgg tgcgtcttat ttagatgaat tttattggtt ggataaggat
    22201 gatcctaatt catccaactg tcgtttgatt cacaagagag gaaatcgtgt ggatgatgac
    22261 ggccagtata cgctcggtaa acagtcaaaa gaattaatcc atttaatcat gaagacagaa
    22321 gaatctctag gagaccaaac cattgaagag ttcttctcag aagatttctt taagagtaat
    22381 ttttgggtgt attgggcaac catgtttgct tttgaaaaat ggcactctgc tgtagaaatg
    22441 cggcgctatg cgatgaggtt tatccaccat attgatggtt tgccagattt tacctccctc
    22501 aagttcaaca aatataacca atatgactct atggtcaaac cgattattgc ttacctagaa
    22561 tcacacgacg ttgacatcca atttgacaca aaagtcactg atattcaggt ggaacaaaca
    22621 gctggtaaaa aggtagcaaa aaccatccat atgacggtgt ctggggaggc taaggcgatt
    22681 gagctaacac ctgatgattt ggtttttgtg accaatggtt ctattactga aagcagcaca
    22741 tacggtagtc atcacgaagt ggctaagcca accaaagcgt taggtggttc ttggaattta
    22801 tgggaaaatc tagctgctca atcagatgat tttggtcatc ctaaagtgtt ttaccaggac
    22861 ttgcctgctg aaagctggtt tgtgtctgcc acagcaacca taaaacaccc agctatcgag
    22921 ccttatattg aacgtttgac ccaccgtgac ttgcacgatg gcaaagtgaa cactggcggc
    22981 atcatcacta ttacagattc taactggatg atgagctttg ccattcaccg tcaacctcat
    23041 tttaaagaac aaaaagaaaa tgagaccact gtctggattt acggtcttta ttccaatagt
    23101 aagggcaatt acgtccacaa gaaaattgag gagtgtacag gtcaagaaat cacagaagaa
    23161 tggttgtacc accttggggt acctgttgat aaaatcaagg acttagcgag tcaggactat
    23221 atcaatacag ttcctgttta catgccttat attacgagtt actttatgcc acgcgtcaaa
    23281 ggagaccgtc cgaaagttat cccagatggt tcagtcaact tggcctttat tggtaacttt
    23341 gcggaatctc catctcgaga tacggtcttt acgactgagt attctattcg tactgccatg
    23401 gaagcagtgt atagcttctt gaatgtggaa cgaggcatcc cagaagtctt taattcagcc
    23461 tatgatattc gtgaattgct caaagccttt tattacctta atgataaaaa ggcaatcaag
    23521 gatatggatt tgccaattcc tgcactgatt gagaaaatcg gacataaaaa aatcaaggat
    23581 acctttatcg aagaattgct caaagatgct aatcttattt aacatgcctt agagtagtta
    23641 aagctgataa gtttaggctt atcagctttt ttgatgattt atagaaaagt gataaaggaa
    23701 aagtctgacg tgaacgtcac ataagaattt aaaatttgtt ataatgtaag caataaagat
    23761 gaaagaaaga gggactctat ttgcaagagt attctattga cattacctta acccatcctg
    23821 atgatgtttt agctcttttt ggtagcaacg agcgtcattt gaagctaata gaagctcatt
    23881 taggagttat tgttcacgca agaaccgagc gagtccaagt gattggtgat gatgaagaag
    23941 ctgtggaatt ggctcgattg accatcaaag ccctactggt tctagtgggt cgtggcatgg
    24001 tggtcaacac atcagatgtg gtgacagctt tatctatggc agaatctcat cagattgacc
    24061 aatttatggc gctttatgaa gaagaaatca ttaaagataa ctatggtaag gcaatacgag
    24121 tcaaaacatt aggtcaaaaa acctatgtcg atagtgtcaa acgtcatgat gtggtttttg
    24181 gtgtaggacc tgcagggaca gggaaaacct tcttagctgt tacacttgca gtcactgctc
    24241 tgaaaagagg acaggtaaag cgtattattt tgacgcgtcc tgcggtagaa gctggtgaaa
    24301 gcctaggttt tttaccaggc gatttgaaag aaaaggtgga tccttacctc agaccggttt
    24361 acgatgccct ttatcacatt ttaggcaagg aacaaaccac acgcctgatg gaacgagatg
    24421 tgattgagat tgctccgtta gcttatatgc ggggtcgtac tctggatgac gcttttgtta
    24481 tcttagatga ggctcaaaac acaaccatca tgcaaatgaa gatgtttttg acccgtcttg
    24541 gttttaattc taaaatgatt gtcaatggag atacgagtca gattgacctc cctcgaaatg
    24601 tcaagtctgg tttgattgat gctactcaga aattacaagg cattaaacag attgattttg
    24661 tctatttctc tgctaaggat gttgtccgac atcctgttgt ggctgatatt atcaaggctt
    24721 atgaaacgtc ctcagaggag acgaaggact tactaaaacc taaggctagt gacgttgaag
    24781 ctgattacca gccaggcttg acaaaatacc ctgtgatcgg tcaagagcac taagactttt
    24841 acgatgatgt ttaatatggc ctttttaagg aggattctat ggatgattta accaaagcta
    24901 ttatggcaga tgaggctaat ctctcctata ccgaaagagg tatttttcct ttatatgatg
    24961 ctcctcaaac agctaggatt attattgttg gccaagctcc tggaatagta gctcaagaaa
    25021 ccaaacttta ctggaatgat cgtagtggca tccgcttacg agactggcta ggtgtggaca
    25081 atgacacctt ttaccactca ggattgtttg gcattatccc tatggatttc tattatcctg
    25141 gtaagggaaa atccggtgac ttaccaccta gagaaggttt tgcggctaag tggcacccgc
    25201 cattaagagc attgatgcca gaagttgaat tgacgatttt ggttggtcgc tatgctcagg
    25261 acttttacct tggtaacaag gcttacaaaa ccttaactga aacagtccgt cactttgagg
    25321 agtacttgcc agactatttt ccacttgttc atccttctcc aagaaaccaa ttgtggttag
    25381 ctaagaaccc ctggtttgag caggatttat tacctatctt gcaaaaacgt gtggaggcaa
    25441 ttctaaccaa ataggccctg atttacctag cattcccatt tttaaggggc ttgtggtata
    25501 atgggtaaat aacaaatgct gattgaaagg ataagggaaa ttatctgtca gataataatg
    25561 ggtgcccaac ataccaaatg aggtgggcag cccttcccta acaattaacc ttatgtatat
    25621 cgagatgatt gacgaaacgg gacaagtttc gcaagagatt atggagcaaa cacttgattt
    25681 gctcaatttt gctgctcaaa agacgggcaa agaagaaaaa gaaatgtctg tgacctttgt
    25741 gaccaatgaa agaagtcatg aactcaactt agaataccga gatactgatc gtccaacaga
    25801 tgtgatttct ctagaatata agccagaaac gcctatttta tttagtcaag aggatttggc
    25861 tgctgatcct agtttggcag agatgatggc agagtttgat gcttatattg gcgaattgtt
    25921 tatttctatt gacaaggcgc gtgagcagtc ccaagaatat gggcactctt ttgagcgtga
    25981 aatgggattt ttagctgttc atggattctt acatattaat gggtatgatc actacaccct
    26041 tgaagaagaa aaagagatgt ttaccttaca ggaagagatt ttgactgctt atggccttac
    26101 acgacaataa taccaccaaa cggaaatgga aaaacagaac cattacctca agcttggagt
    26161 ttgccttgac aggggttttc acagctttta aggaagaacg caatctcaga agccaccttt
    26221 tgtctgcctg tttagcttgt gttgctggtt tattttttag catttcagcc atcgaatggt
    26281 tatttttact tctagccatt tttttggtca ttaccttaga aattgtcaat tctgctattg
    26341 aaaatgtagt tgatttggct agtgactatc acttttctat gctagctaaa aatgccaagg
    26401 atatggcagc aggtgctgtt ttgatgattt ctggctatgc tgtcttgaca ggacttatta
    26461 tttttattcc taaaatttgg aatattttcg ttcattaaaa agaaaggaat gggctatcct
    26521 atgtgatgat ggcataggag aataaggaaa ggacaagacg gttaagctta gtgacaagct
    26581 tgatctgaag ccctaccgtt aaggcccgta attatctatg tttaaatcag gctttgtagc
    26641 cattcttggt cgtccaaatg ttggcaaatc caccttttta aatcacgtca tggggcaaaa
    26701 aattgccatc atgagtgaca aggctcagac cacccgtaat aaaattatgg gcatttacac
    26761 cacagaaaca gaacaaattg tctttattga tacgccaggt attcataaac caaagaccgc
    26821 ccttggtgat tttatggttg aatctgctta tagtacttta cgtgaagttg agacagttct
    26881 tttcatggtt cctgccgatg aaaagcgtgg taagggtgac gacatgatta tcgagcggct
    26941 aaaggcagct aagattcctg ttattttagt catcaacaaa attgataagg ttcatccaga
    27001 ccaacttttg gaacagattg atgacttccg tagccaaatg gattttaaag aagtggtccc
    27061 aatttctgcc ctagaaggta ataatgtgcc aaccctaatc aagttattaa ctgataatct
    27121 tgaagaaggt ttccaatatt ttccagaaga tcagattact gatcatccag aacgtttcct
    27181 tgtgtcagaa atggttcgtg agaaggtact gcatttgacg caacaagagg ttccacattc
    27241 cgtagctgtt gttgtcgaat ccatgaagcg agatgaagag actgataaag tgcatatccg
    27301 tgcaaccatt atggtggagc gcgatagcca aaaaggcatc atcatcggta aacaaggagc
    27361 catgctcaaa aaaatcggaa aaatggcgcg ccgtgatatt gaactcatgt taggtgacaa
    27421 ggtctactta gaaacgtggg tcaaagtcaa gaaaaactgg cgggataaaa agcttgattt
    27481 agctgatttt ggctataatg agaaagaata ctaacatagt aaagcgagat catatgccac
    27541 aagactacat ttcttatatc cgctccaaag ttgggcatga taagattatt cttaactttg
    27601 ctggtgggat tttgaccaat gatgacggca aggtgctgat gcagttgcgt ggtgataaga
    27661 aaacctgggc tattcctggt ggcactatgg aactgggtga gagctcttta gagacttgca
    27721 aacgtgagtt cttagaggaa accggaattg aggttgaagc tgttcgctta ctgaatgttt
    27781 acactcattt tgaggaagtt tatcccaacg gtgatgctgt tcagactatt gtctttatct
    27841 atgaactaac ggctgtttct gatatggcta ttgataactt tcataatgag gaaacgctca
    27901 agttacagtt tttctctcat gaggagatag cagagttaga gagtgtctca gccaaacatc
    27961 gcctgatgtt agaggaatat tttagtgata gctttgctat ggggcattaa gaactctcta
    28021 ggacagttgg agtatttttg gagattggct tgttaaaaaa atttttgaaa gacgatgttt
    28081 cgagagctta tgctctcttt ttttgcttcc aatttttctg attaagtctg aataattttg
    28141 ttaaatgtta acaggatttt ctttccaatt gatactagca gacatcaaac atttgtttta
    28201 gagttatgac gaattgccac ctagccatgc atgacttagg caagttgaac ctaactgtgg
    28261 caatagactt ggaaggggtg acaagaatca tttcctaaaa gaattgatga agtgcctatc
    28321 cattattcga attgtaaagt tcgtctaagt aaagttgaat ttgatctcga atggactttg
    28381 atgatttatg acttgttgtg tcaataacac caaaaactcg ataatgattt ttaaatattt
    28441 cctcgtaatt aatatgattt ccgattagaa taatatgagc atgaggataa cgcatacgta
    28501 tttcgtttag aaggtcatat acttgctcag atttaaaaaa gtatgattta tgataaaata
    28561 aacctgacat ttgtatttat aatctaagtt tttcccacta ataatttgag tttttagaaa
    28621 gtatc
//
LOCUS       DAUDYX010000021        28021 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.021, whole genome shotgun sequence.
ACCESSION   DAUDYX010000021 DAUDYX010000000
VERSION     DAUDYX010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28021
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.021"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(389..820)
                     /locus_tag="VHD02_000911"
     CDS             complement(389..820)
                     /locus_tag="VHD02_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497529.1"
                     /translation="MKQQSYQPLRFVYLLVALFAAMLLIARPVMADGENNDPANAAYY
                     KGKSAGEEAGKTAGKEASWTDLTPTNPTIPEPPSDIRETTNKQHYKDGYTDGYRDGYN
                     EGWKSQHPVLTPVKVIWDLISHWLQQFFPNNQATTTVQSMS"
     gene            complement(1075..2790)
                     /gene="slo"
                     /locus_tag="VHD02_000912"
     CDS             complement(1075..2790)
                     /gene="slo"
                     /locus_tag="VHD02_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_095889392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin streptolysin O"
                     /protein_id="HEQ5497530.1"
                     /translation="MSNKKTFKKYSRVAGLLTAALIIGNLVTANAESNKQNTASTETT
                     TTNEQPKPESSELTTEKAGQKMDDMLNSNDMIKLAPKEMPLESAEKEEKKSEDKKKSE
                     EDHTEEINDKIYSLNYNELEVLAKNGETIENFVPKEGVKKADKFIVIERKKKNINTTP
                     VDISIIDSVTDRTYPTALQLANKGFTENKPDAVVTKRNPQKIHIDLPGMGDKATVEVN
                     DPTYANVSTAIDNLVNQWHDNYSGGNTLPARTQYTESMVYSKSQIEAALNVNSKILDG
                     TLGIDFKSISKGEKKVMIAAYKQIFYTVSANLPNNPADVFDKSVTFKELQRKGVSNEA
                     PPLFVSNVAYGRTVFVKLETSSKSNDVEAAFSAALKGTDVKTNGKYSDILENSSFTAV
                     VLGGDAAEHNKVVTKDFDVIRNVIKDNATFSRKNPAYPISYTSVFLKNNKIAGVNNRS
                     EYVETTSTEYTSGKINLSHQGAYVAQYEILWDEINYDDKGKEVITKRRWDNNWYSKTS
                     PFSTVIPLGANSRNIRIMARECTGLAWEWWRKVIDERDVKLSKEINVNISGSTLSPYG
                     SITYK"
     gene            complement(2814..3299)
                     /locus_tag="VHD02_000913"
     CDS             complement(2814..3299)
                     /locus_tag="VHD02_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD glycohydrolase inhibitor"
                     /protein_id="HEQ5497531.1"
                     /translation="MYKVPKSLEHYQKMFQKEVTVNDLKKYLIGSDKEYRITRRDSYM
                     GDISDPEVILEYGVYPAFIKGYTQLKANIEEALLEMSNSGQALDIYQAVQTLNAENML
                     LNYYESLPFYLNRQSILANITKALKDAHIREAMVHYKLGEFAHYQDTMLDMVERTIET
                     F"
     gene            complement(3304..4659)
                     /gene="nga"
                     /locus_tag="VHD02_000914"
     CDS             complement(3304..4659)
                     /gene="nga"
                     /locus_tag="VHD02_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotine adenine dinucleotide glycohydrolase"
                     /protein_id="HEQ5497532.1"
                     /translation="MRNKKVTLAHIVAKTSVAIALAGAMGSSLLANSTTYAVSGKENK
                     KSDVKYETTKVMEANATSSKEDNHVMHTLDGSMSTVWEENSPGGGVGEVLSYKFVSPM
                     RIGRILIVNGDTSSKENYYKKNRIAKADVKYYNGNKLVLFQKIELGDTYTKKPHHIEI
                     DKKLDVDRIDIEVTEVHQGQNKDILALSEVTFGNMERDLFEKKFKEIKDKWVTDKQAD
                     EFIETADKYADKAVQMSAVASRAEYYRMYVSRKYHYKKEFVEKLKQVYKESGASHVTS
                     KKDLMLAFDDAKRKSTIGRQENGLFVTSFAEDMALLFTDQGKLKSADQIENIKGVDSG
                     KYSDGVYQYEYDSELTKNIDKLGYIRTASGDTPGANSLNIPGCQTWSGKHIENSESEL
                     IFPSISVKDLKSKAVLAEIDAKGYFEIIDPTIIAPNGDHKKVTGRFKIKKMQDRMQDR
                     K"
     gene            complement(5030..5569)
                     /gene="nusG"
                     /locus_tag="VHD02_000915"
     CDS             complement(5030..5569)
                     /gene="nusG"
                     /locus_tag="VHD02_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEQ5497533.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGQTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRAILLSMGQTIDVFDTNIKEGDVVQIIDGAFMGQEGRVVEIENNKVKLML
                     NMFGSETVAEVELYQIAEL"
     gene            complement(5742..6785)
                     /locus_tag="VHD02_000916"
     CDS             complement(5742..6785)
                     /locus_tag="VHD02_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEQ5497534.1"
                     /translation="MNKKVMSLGLVSTALFTLGGCTNNSSKQTTDNSLKIAMITNQTG
                     IDDKSFNQSAWEGLQAWGKENKLEKGKGYDYFQSANESEFTTNLESAVTNGYNLVFGI
                     GFPLHDAVEKVAANNPDNHFAIVDDVIKGQKNVASITFSDHEAAYLAGVAAAKTTKTK
                     QVGFVGGMEGDVVKRFEKGFEAGVKSVDDTIKVRVAYAGSFADAAKGKTIAAAQYAEG
                     ADVIYHAAGGTGAGVFSEAKSINEKRKEEDKVWVIGVDRDQSEDGKYTTKDGKSANFV
                     LTSSIKEVGKALVKVAVKTSEGQFPGGQITTFGLKEGGVSLTTDALTQDTKKAIETAK
                     KAIIEGTITVPEN"
     gene            complement(7112..8404)
                     /locus_tag="VHD02_000917"
     CDS             complement(7112..8404)
                     /locus_tag="VHD02_000917"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEQ5497535.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPQKISVIGNGVVVNPKSLVKELAYLHDEGVTTDNLRISDRA
                     HVILPYHIQLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFAERL
                     RINLAEKNRLFEKMYDSTPLGFDAIFEEYYAYGQEIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGKRIDYYPASLEQLKRCKPIYEELPGWQEDITGVRSLDELPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWASI"
     gene            8842..9720
                     /locus_tag="VHD02_000918"
     CDS             8842..9720
                     /locus_tag="VHD02_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497536.1"
                     /translation="MIRKYDRTSTKKKSLNWIWLIIAFFMISSFIGGSSFTESLLDIL
                     PAIAIGGTGYAIFRVRSHQKRLAKAKIAKQLEDLKAKIQLADRKVRLLDTYLADHDDF
                     QYNVLAQQLLPQLSDIKAKAITLKDQLDPKIYRRITKKANDVESDITLQLETLQIATT
                     LNPQPLKTPSPNLINKAPELKPYYDNIQTDHQAILAKIQGADNQEELLALHEANMRRF
                     EDILTGYLKIKEEPKNYYNAAARLEQAKQAIQQFDEDLDETLRRLNESDLKDFDISLR
                     IMQGATQRRTTHHQKD"
     gene            9733..10995
                     /locus_tag="VHD02_000919"
     CDS             9733..10995
                     /locus_tag="VHD02_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEQ5497537.1"
                     /translation="MADFNFDIDQIADNAVVKTDKTTDIISDLPTDTNGQISFFEKLS
                     ADQQTAITAKAPALVDTFLADQNALLDFGQSAVEGVNATVNHILAEQKKLQIPQVDDL
                     LKSTNRELNGFIAKYKDATPVDLDKKPNFLQKLFKQSRDTLQEFYFDSQNIEQKMDSM
                     AAAVVKQEDTLARNIVSAELLIEDNTKSIEHLVGVIAFIEASQKEASQRAAALQKDLK
                     TKDSATPDYQIKADLLARTTEVINTLEQQHTEYLSRLYVAWATTPQMRNLVKVSSDMR
                     QKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGMTADAIINANNAALQMLAETSKEAIPA
                     LEQSAQNPTLSMKSVTSLAESLVAQNNGIIAAIDHGRKERAQLESAIIRSAETINDSV
                     KLRDQNIVQALLSEGKETQKTIDKTTHA"
     gene            complement(11115..11741)
                     /locus_tag="VHD02_000920"
     CDS             complement(11115..11741)
                     /locus_tag="VHD02_000920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEQ5497538.1"
                     /translation="MARLNVKPTRMELSNLKNRLKTATRGHKLLKDKRDELMRRFADL
                     IRENNELRQTIEKELAANMKEFVLAKASENSLMVEELFAVPVHEVTLWIDIENIMSVN
                     VPKFHVQSNTAREQEQGEFAYSYLSSNSEMDNTIQKTKELLEKLLRLAEVEKTCQLMA
                     DDIEKTRRRVNGLEYSIIPQLEETIHYIELKLEEAERASLVRIMKITS"
     gene            complement(11786..13201)
                     /locus_tag="VHD02_000921"
     CDS             complement(11786..13201)
                     /locus_tag="VHD02_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEQ5497539.1"
                     /translation="MSVLKEYRTVSEVVGPLMIVDQVAGVHYNELVDITLHNGERRKG
                     QVLEVQGDKAMVQLFEGSTGINLAKTKVRFTGHPLELAVSEDMVGRIFDGMGQPIDGG
                     PELIPEKYLDIDGQAINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDDNFAVVFAAMGITFEEAEFFMNDLRETGAIDRSVLFINLAND
                     PAIERIATPRIALTTAEYLAYEKGMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLIGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSQE
                     LYKNGFRPPINVLPSLSRLKDKGSGEGKTRQDHAATMNQLFAAYAQGKQAKELAVVLG
                     ESALSETDKLYVAFTNRFEEEYINQGFYTNRSIEESLDLGWELLSILPRTELKRIKDD
                     MLDRYLPKADTTMTKVFVAND"
     gene            complement(13202..14977)
                     /locus_tag="VHD02_000922"
     CDS             complement(13202..14977)
                     /locus_tag="VHD02_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEQ5497540.1"
                     /translation="MNQGKIITVSGPLVVASGMQEANIQDICRVGHLGLVGEIIEMRR
                     DQASIQVYEETSGIGPGEPVVTTGCPLSVELGPGLISEMFDGIQRPLDRFQKATDSDF
                     LIRGVAIPSLDRKAKWAFIPKLSVGQEVVAGDILGTVQETAVIEHRIMVPYKVSGTLV
                     AIHAGNFTVTDTVYEIKQEDGSIYQGSLMQTWPVRQSRPVAQKLIPVEPLVTGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQIAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLQEMPGDEGYPAYLGSRIAEYYERAGRVRTLGSQEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYQDDVGSYIDRKQ
                     ESNWSNKVTRAMAILQREASLEEIVRLVGLDSLSEQDRLTMAVARQIREDYLQQNAFD
                     SVDTFTSFPKQEAMLTNILTFNEEASKALSLGAYFKEIMEGTAQVRDRIARSKFIPEE
                     NLEQIKGLTQKVTKEIHHVLAKGGI"
     gene            complement(15178..15498)
                     /locus_tag="VHD02_000923"
     CDS             complement(15178..15498)
                     /locus_tag="VHD02_000923"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEQ5497541.1"
                     /translation="MTKTLYKVGVIGNRDVILPFQMIGFQTFPVIKPQDAINQLRQLA
                     MEDFGIIYITEDIAAAIPEALTHYDNQVLPAVIPLPTHQGAQGIGLSRIQAMVEKAVG
                     QNIL"
     gene            complement(15495..16493)
                     /locus_tag="VHD02_000924"
     CDS             complement(15495..16493)
                     /locus_tag="VHD02_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEQ5497542.1"
                     /translation="MPTFLELNTTISVKEKELLTKEQFDKLLQAPNTTTLARLLHQSV
                     YHLTVDDLNDLDRLESILMAELTKTYRWAFAETPQPDIVQLFTLRYTYHNVKVLLKAK
                     ASQADLSHLLLPIGDKPLVALEHLIRTMTSDEFPKEVVTEIQSIWAEYQDYQDIRVLE
                     IGTDLAYFKALKQIAQRLEDPVFQQAVLIVIDLYNLITVRRAKSQNKPISFMMQLLSD
                     EASRPSKTFITLEDDKDLMTWFENVTPDSYMTALKPYSEKLRQGTLQTTELEYLVDEC
                     LYHLFAKAKYQVDGPYVLARFLLAKSFEVKNLRLLVAALANDLPKERVIERMRPIA"
     gene            complement(16509..17093)
                     /locus_tag="VHD02_000925"
     CDS             complement(16509..17093)
                     /locus_tag="VHD02_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEQ5497543.1"
                     /translation="MNDITQLRQNVLEKAHQEGQQCLKIATDSLDTDFKERQQQGLHD
                     LKAKRQKELKALEQQFQVAQQQLKNQERQALLALKQDSIKGLFEASLEKMTNFSKEEE
                     LAFLKQVLSKYPEQPLQVTFGEKTGQKFSSYDCAELRLAFPQLSYNQELIPQEAGFLV
                     SLDQVDDNYLYRYLLESVLKEESSRIIDMLFSEI"
     gene            complement(17162..17641)
                     /locus_tag="VHD02_000926"
     CDS             complement(17162..17641)
                     /locus_tag="VHD02_000926"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEQ5497544.1"
                     /translation="MEHLAHYFTAHGGVFFAALGIVLAVALSGMGSAYGVGKGGQAAA
                     ALLKEEPEKFTSALILQLLPGSQGIYGFAIGILIWMKLTPELSVNQGLAYFLISLPIA
                     IVGYFSAKHQGNVSVAGMQILAKRPKDFMKGVILAAMVETYAILAFVVSFILLNKIG"
     gene            complement(17643..19643)
                     /locus_tag="VHD02_000927"
     CDS             complement(17643..19643)
                     /locus_tag="VHD02_000927"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEQ5497545.1"
                     /translation="MAISQMKKLAMVFEKDYLDLVLKTLQQSQLVEVRDMKQLKHWQD
                     AFNKGNVKLPQIVQYDLTHQKPLLDDEALQYLLQSQQELENGLASLSAFLPPIGKLTA
                     LRQKTPSLSFKQFEESHRQQAAQTALKMMSQKIERLEQLQNKIDQLTEYCQELEKWRS
                     LTVLPQDLAQFHFLSARVGTIPSTANDYFYHQLKQHKGLFIEEVYHTEFEYGLVLFWQ
                     AQDTIHLQKYQFKPLLYKEQLLPSEQLRINKELLTNWLAEKDSLLKELRQSQKILAQL
                     QVEIDYVLSQYQRQQTKKQLLGTRHLIALEGWIEADSVNQLKGLMTKTLGDMFYLDSY
                     DVTPDDWEDVPIKLRNHRYIAPFELVTEMYALPKYQEKDPTPFLAPLYLTFFGMMVAD
                     LGYGLLLYAVTLAALVFFNLQKTSKRLVTFFNILAISVAIWGLIYGSFFGFDLPVALL
                     STKTDVITILVVSLLFGFVTLIFGLLLGAWQQVRMKAYATAYTSSLAWTFILLGLLLF
                     ILGKNVSGLAYLSVIGKWLALGNAFGILVVSLLKSKSLLGLGSGLYNLYGISSYLSDL
                     VSFTRLMALGLSGASIGAAFNMIVGIFPPVTRFTVGIFIFILLHAINIFLSMLSGYVH
                     GARLIFVEFFGKFYEGGGKAFNPLKLADNYVNVNEETDLEDN"
     gene            complement(19633..19953)
                     /locus_tag="VHD02_000928"
     CDS             complement(19633..19953)
                     /locus_tag="VHD02_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497546.1"
                     /translation="MNTLLSLMNSIEQEARTIYDSYDAKKKSLADKKEEELKQLFLEY
                     EQETQLLLEAKREVFFEANRQEQKASKKRLATQQLALHAILEEHKLTVVRELVDKVVE
                     TYGH"
     gene            complement(20407..21426)
                     /locus_tag="VHD02_000929"
     CDS             complement(20407..21426)
                     /locus_tag="VHD02_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEQ5497547.1"
                     /translation="MDIIIGTSLLILVLAIFSLFNYKAPHGAKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVAIAIGVSPGYAVLIGLSVSGTG
                     IIPGFVAGYVVSFLIKWMEKNIPGGLDLISIIIVGAPLTRFLAQLITPVINSTLLTIG
                     DILTSSANSNPIIMGMILGGTIVVVATAPLSSMALTAMLGLTGIPMAIGALSVFGSSF
                     MNGVLFYRLKLGERKDNIAFAIEPLTQADVTSANPIPIYVTNFVGGAACGVLIALMKL
                     VNDTPGTATPIAGFAVMFAYNPVAKVLITALGCIIISLIVGYIGGSVFKNYRLVTKQE
                     LQARN"
     gene            complement(21463..21828)
                     /locus_tag="VHD02_000930"
     CDS             complement(21463..21828)
                     /locus_tag="VHD02_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEQ5497548.1"
                     /translation="MKSYPEPMGPYSTYTIEGHFLYTAGQLPLNPVTGQLSDGFEAQC
                     RQVFVNLQSILAEQKLDLNHIYKLNVYLTDVTNVEILNHVMTDLFEEPYPVRTAVQVS
                     ALPLQALIEVEAVARVKQL"
     gene            22002..22682
                     /locus_tag="VHD02_000931"
     CDS             22002..22682
                     /locus_tag="VHD02_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ5497549.1"
                     /translation="MDKETLNYWKTVITFLHDVLGDNYEIILHVIDKNDIYIGELVNS
                     HISGRSKQSPLTTFALDLITNKVYKEKDFVTNYKAIVSPQHKEVRGSTFFIKDKKGNL
                     EGMLCINLDISAYQGVARDLLKLVNLNLEHFIPTAKEPKTVTPQPEEAVEILTSNIQD
                     IIGQIIDPSLLRHNVHLSQDVKIDIVAKLYEKGVFQLKGAVSKVADILCISEPSVYRY
                     LKKIEADQ"
     gene            complement(22804..23451)
                     /locus_tag="VHD02_000932"
     CDS             complement(22804..23451)
                     /locus_tag="VHD02_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEQ5497550.1"
                     /translation="MAVALSKEEIQNRIAKRVAKELEDGTLVNLGIGLPTKVANFVPE
                     EMTVYFQSENGFIGLGPKSDDPNSTIVNAGGQPVTVYPGAAFFNSADSFGIIRGGHVD
                     LTVLGALEIAENGDIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKHKLLKE
                     CTLPLTAKGVVDLIITEMGVFKVTPDGIQVIEISEGFTFDEVQAATGVPLSIAEG"
     gene            complement(23454..24113)
                     /locus_tag="VHD02_000933"
     CDS             complement(23454..24113)
                     /locus_tag="VHD02_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEQ5497551.1"
                     /translation="MKENKRIAIAEAISHIKDGDTIMVGGFMANGTPEALIDALVDKG
                     TKDLTLICNDAGFVDRGVGKMVANHQFKTIYATHIGLNKEAGRQMTAGETTIELIPQG
                     TFAEKIRIGAYGIGGFYTPTGVGNLVAEGKETKTIKGKTYLLEYPFEADVALIFANKA
                     DEMGNLQYSGSENNFNQLMAACAKTTIVQAREIVPVGTIQPECVHTPHIFVDYIVKEG
                     V"
     gene            complement(24125..25309)
                     /locus_tag="VHD02_000934"
     CDS             complement(24125..25309)
                     /locus_tag="VHD02_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEQ5497552.1"
                     /translation="MTKEVVITSAYRTPIGNFGGVFKSLSAVDLGVTVVTKILADTGL
                     KSDAIDEVIFGNVLHAGLGQNVARQVALNAGLSYDTPAFTIDMVCGSGLKAVELGAQK
                     IQTGNADIVLVGGTENMSQAPYVLQGQRWGSRMGDSKVVDTMLKDGLSDAFAGYHMGI
                     TAENIVQQYGLTREEQDAFAADSQRKAQLAIEKGRFKEEIAPVTIPQRKGEPLLVDQD
                     EYPKFGTTVDKLAKLRSAFIKDEGTVTAGNASGINDGAAAILLMSKEKAEELGLPILA
                     KITSYASAGVDPSIMGCGPIPATKKALAKAQLTIDDIDLIEANEAFAAQALAVSRDLG
                     FDNEKVNVNGGAIALGHPIGASGARILVTLLAEMAKRDVRHGLATLCIGGGQGQSIIV
                     TR"
     gene            25430..26341
                     /locus_tag="VHD02_000935"
     CDS             25430..26341
                     /locus_tag="VHD02_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ5497553.1"
                     /translation="MNISSLAYFIEIVNCDFNLTKAAQKSHISQPALSSYIKTIEKEE
                     GVVLFQRQKGRLVGLTSVGERFFENAKVVIASHEQLMADLRSESKKYKGEISIGIPQL
                     ISGIVFSELIPKLIEENPEVKFHIVETGAYELQKKLFLKEVDFAILLTPTTLDATIAD
                     IDILLRDQLCLYMSHKTFQETFDSNPNISIKALHRQNLVTFDKTFMINHQLTTLFEEA
                     NSLPVVKCYSASWDFLLNCTRYSSYLTILPRPITHFAHMDGLVEVQLTEHPKWEVVLA
                     SLKHNKTSHLKHYIKHTILDYFTNQRF"
     gene            complement(26409..27815)
                     /locus_tag="VHD02_000936"
     CDS             complement(26409..27815)
                     /locus_tag="VHD02_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acid transporter"
                     /protein_id="HEQ5497554.1"
                     /translation="MNNIKTKKRFMDRYIDGFMKWMPESLFICFILTFLVVTMSVLMT
                     DSPFIGTEKTGGIIYGWVNGFWGLLSFAMQMTILLATGNAVASSPPAHKMFKSLAKLP
                     QTRTQIFIFSIVVGSIFGFLHWGLGMMVAIVFGKELLVQARQKGIKVHTPLFVATLFF
                     TFLPATSGLSGAAVLYSATPDYLRNSVADAYKQVVPESVPLTESVLNLPFISLLVVCM
                     LVPLCFALLAHPKDETKIMELDDEIYHHSLDTASHVVIARNTPAEKMNASRLVMYLVG
                     GAIVSYSLYHFSVVGLSGLDLNSFNFLFLGLGLLLCGQQGPEYYGSLFKDGVMSSWGL
                     VLQFPFYAGIFGIIQSTGLGLEISHFFVAISNGTTWPVFAYLHSALLNIAVPSGGSKF
                     VIEAPYIVPATIEVGNDLGKILQAYQLGDATTNLIVPFWALSYLSNFKLKFNQIVAYT
                     IPCVLVVTGIAIIYLFVF"
BASE COUNT         8432 a         5997 c         4931 g         8661 t
ORIGIN      
        1 ctgctgctgt cgtaagcctt agccttgcaa caccagaaaa ccaaactgtt tcagcaaatg
       61 acacgcaagt tacttgtgta ggatgtccag attgtggtcc aaaatggtct tgttgtccat
      121 tgacctgtat gcgcgatttg ttaagaggtt ggggcaagtc agcagcatat tattgcactt
      181 ggggatatta ctactctgag taaaaattac ttgagagtct gagacagtag tctcttgatt
      241 atgctaaaaa aacaatggct ttgacgccat tgtttttttg tgtggccgag aggctttttc
      301 acaaactctt tttttattgt tcattcccat atcctaaaaa aacaaccttt cacattagca
      361 gttaaaggct gtttttaatt catttttatt atgacatgct ttgtactgtt gtagttgcct
      421 ggttattggg gaagaattgc tgtagccaat gagagatcaa atcccatata accttgaccg
      481 gagtcaagac gggatgctga gatttccagc cttcattata accatctctg tacccatctg
      541 tatacccgtc tttataatgc tgtttattag tagtctctct gatatcacta ggtggttctg
      601 gaatggttgg attagttggg gtcaaatcag tccaactagc ttcttttcca gctgttttcc
      661 ctgcttcttc tccagcgctt ttccctttat aatatgctgc attagctggg tcattattct
      721 ccccatctgc cataacaggt cttgctataa gcaacatagc agcaaataga gccaccaaga
      781 ggtagacgaa gcgtagaggc tggtaagatt gttgtttcat gataatagtt atctctccct
      841 tttatgttat gctttgcttg ttaaaaagca gcttgttagt attatagatt agaagcagct
      901 tagcttcaag gtcacgatgt gagaaaagaa taattttttg attagtatat caaaaaagct
      961 tgtctatgaa ggtatctcat agacaagctt tcttctgtat ttgtgagagg attaggcaca
     1021 aggcctcaag agataagcag catcaaggtg cttgacgaac ctcttgaacc agtcctactt
     1081 ataagtaatc gaaccatatg ggctcaaggt tgatcctgag atgttgacat tgatttcttt
     1141 agacagtttc acatctcttt cgtcgatcac ttttcgccac cattcccaag ctaagccggt
     1201 gcactctcta gccatgatac ggatatttcg tgaattagct cctagtggga taactgtgct
     1261 aaatggtgat gtcttactat accagttgtt gtcccaacgt cgttttgtaa tcacttcttt
     1321 tcctttgtca tcataattga tttcatccca aaggatttca tattgagcaa cataggcacc
     1381 ttgatgagac aggttaattt ttccactcgt gtactcggta gatgttgttt caacgtattc
     1441 acttctgtta ttgacacccg caattttatt atttttaagg aaaacactgg tgtatgaaat
     1501 aggataagct gggttttttc tactgaaggt agcattatct ttgataacgt ttctaataac
     1561 atcaaagtct tttgtgacta ccttattgtg ctctgcagca tctcctccta aaacgacagc
     1621 tgtaaatgaa ctattttcta aaatatcaga gtattttcca ttagttttaa catctgttcc
     1681 ttttagagct gcactaaagg ccgcttcaac atcattactt ttagaacttg tttctagttt
     1741 gacaaaaaca gttcgaccat aagctacgtt actcacaaag agtggcgggg cttcattgct
     1801 gacacctttt cgttgcaact ctttaaaggt cactgattta tcaaacacat ccgcaggatt
     1861 attaggaagg tttgctgata cggtgtaaaa aatttgcttg tatgctgcaa tcatcacctt
     1921 cttttcacct tttgaaatcg acttgaaatc aatgcctaaa gtaccatcta agattttact
     1981 attaacattt agagctgctt caatctgtga tttagaatat accattgatt cagtatattg
     2041 tgttctggca ggaagcgtat taccaccaga ataattatca tgccattggt taacaagatt
     2101 atcaatagct gttgaaacat tggcataggt agggtcattg acctcaaccg ttgctttgtc
     2161 tcccatacct ggtaaatcaa tatggatttt ttgtgggttt cgcttggtga ctaccgcgtc
     2221 tggtttgttt tcggtaaaac ctttattagc cagctgaagg gctgttggat aggtcctatc
     2281 agtgacagag tcaatgatgg aaatatcgac tggtgtagtg ttgatatttt ttttctttct
     2341 ttcaatgaca ataaatttat cagctttctt aacgccttct ttaggaacaa aattttcaat
     2401 ggtttcacca tttttagcaa gtacttcaag ctcattataa tttagtgaat aaatcttgtc
     2461 attgatttct tcagtgtgat cttcttcgct cttttttttg tcttctgact ttttttcttc
     2521 tttttctgca gattctagtg gcatttcttt gggagcaagc ttaatcatat cgttagagtt
     2581 aagcatatca tccattttct gacctgcttt ttcggtagtt agctcactac tttctggctt
     2641 tggttgctca tttgtcgttg tggtttctgt actagcagtg ttttgtttgt tcgattcagc
     2701 attagcagta acaagattac caatgataag agctgccgtt agtagcccag cgacgcgact
     2761 gtatttttta aatgtttttt tgttagacat gtccttcata cctttttatc attctaaaat
     2821 gtttctattg ttctttcgac catatcaagc atagtatctt gatagtgagc aaattctcct
     2881 aatttgtaat gtaccatagc ctctctaata tgcgcatctt ttaacgcttt ggttatatta
     2941 gctagtatgc tttgacggtt taaataaaat ggcaagcttt cgtaataatt taataacatg
     3001 ttttcagcgt ttagggtttg aaccgcttgg taaatgtcta atgcttgacc gctatttgac
     3061 atttctaata atgcttcttc gatgttagct ttcaattggg tatagccttt tatgaaagca
     3121 gggtaaaccc catactctaa aataacttct ggatcagaaa tatctcccat ataggagtct
     3181 cttctagtga tacgatactc tttgtcacta cctatcaagt atttttttaa atcattaaca
     3241 gttacttctt tttgaaacat tttttggtaa tgttctaaac tctttggcac cttatacata
     3301 ttgttacttc ctatcttgca ttctatcttg cattttctta attttgaagc gaccagttac
     3361 ttttttatgg tcaccatttg gagcaatgat ggtaggatca ataatttcaa aatagccttt
     3421 ggcatcaatc tctgctaaga cagctttaga ttttagatct ttaacactaa tcgatgggaa
     3481 aattaactca ctttctgaat tttcaatgtg ttttcctgac cacgtttggc aaccaggaat
     3541 gttgagcgaa tttgctccag gggtatctcc gctagctgtt cggatatagc ctagcttatc
     3601 aatgtttttt gttagttcag aatcgtactc atattgataa actccatcgc tatattttcc
     3661 gctatcgaca ccttttatat tttcaatttg gtcagctgat tttaacttac cttgatcagt
     3721 aaagagcaat gccatatctt cagcaaaact tgtcacaaaa agaccgtttt cttgtcgacc
     3781 aatcgttgac tttcttttag catcatcaaa agctaacatc aaatcttttt tacttgtaac
     3841 gtgagaagct ccgctttctt tgtagacttg ttttagtttt tcaacaaact cttttttgta
     3901 gtggtatttg cggctaacgt acatccggta atactcagca cgtgacgcaa cagcagacat
     3961 ctgaacagct ttatcagcat acttgtcggc agtttcaata aactcatctg cttgtttatc
     4021 tgttacccat ttatctttaa tttctttaaa ctttttttca aaaagatcgc gttccatatt
     4081 gccaaaagtg acctctgaca aggctaaaat atccttgttt tgtccctgat ggacctctgt
     4141 tacctcaata tcaatacgat caacatctaa ttttttatca atctcaatgt gatgcggttt
     4201 tttagtgtag gtgtcgccta attcaatttt ttgaaagagg accaatttat tcccgttata
     4261 gtatttaaca tcagcctttg caattctatt tttcttgtag taattctcct tgctagatgt
     4321 atctccattc acaattaaaa ttctcccaat acgcatcggg gaaacaaact tgtaggaaag
     4381 tacctcacca acgccaccac caggtgaatt ttcctcccag acagtactca ttgagccgtc
     4441 taatgtgtgc atgacatgat tgtcttcttt agaggaagtt gcgttagctt ccataacttt
     4501 ggtcgtttca tatttgacat cgcttttttt attttctttg ccactaacag cgtacgttgt
     4561 gctattagct aataaactgc tacccattgc tccagccaaa gcaatagcaa cacttgtctt
     4621 tgcgacaata tgagctaatg ttactttttt gtttctcatg taaaccacct tatattattt
     4681 ttaagtaaac tatacattaa caactagttt ttgtcaatgc gatttattaa catcgtttta
     4741 ggcttttcta tcaaaaaaat gaacacaaaa aaacaagtag ccaactgtta caagtttcaa
     4801 ggggatattt ccatcttatt atcacagaac cttcacatct gttctattta aaccagatta
     4861 aaagtactag ttaaatattt gatagtagca ccgttatcag taatagatct gttaattatt
     4921 tctgattaca atgatgttaa caatagtcat ccattggttt gtaaacacgc ttcactgcca
     4981 actaaaaaag gtctgatggt aatacttata ccatcagacc tttgagagcc tatagttctg
     5041 caatttgata aagttcaact tctgctactg tttcagagcc aaacatgttg agcatcagct
     5101 tcactttgtt gttttcgatt tctaccacac gaccttcttg tcccataaag gcaccgtcta
     5161 tgatctgaac cacatcacct tctttgatat tggtatcaaa gacatcaatg gtttgaccca
     5221 tagacaacaa gatagcacgg atttcttctt caagaagtgg tgttggtttt gatcggttcc
     5281 cgtgtgatcc cacaaaacct gtaacgtttg gtgtgttacg cacaacaaac caagcttcat
     5341 ctgtcatgac catttcaacc aagacatacc ctggaaagcg attttcttcg atttctttgg
     5401 tttgcccatt tttttcaacg ttaaccgttt gtgtcggaat ttcgacgcgc aaaatattgt
     5461 ccaacatgtt ataggtttgg gcacgttgca agaggttttc ttttactttg ttttcgtagc
     5521 ctgagtaagt ttggaggacg aaccagccct tatcaaatga atctagcatg atgtttcctt
     5581 tcattataaa aaagcccttt ttttggcttt tgcgctgttt tctatcacct tcattataat
     5641 aaaagacagg gctggtgtca acgtatctgt ggagctactt gacaccttta ttaaaaagtc
     5701 caaagtcggt tcgtgacgac tctggacggt attgtacagt tttagttttc aggaactgtg
     5761 atggttcctt cgataatcgc ttttttagca gtctcaatag cttttttagt gtcttgtgtc
     5821 agagcatccg ttgtaaggct aacaccacct tcttttaaac caaaagtggt tatttgacca
     5881 cctgggaatt ggccttctga ggtttttacg gctactttta ctaaagcttt tccgacttcc
     5941 ttgatacttg aggtcaaaac aaaattagct gacttgccat cttttgtggt gtattttcca
     6001 tcttcacttt ggtcacggtc aacaccaata acccaaacct tatcttcttc tttacgtttt
     6061 tcgttgatag acttagcttc gctaaagaca cccgcccctg tgcctcctgc tgcatgataa
     6121 ataacatctg cgccttcagc atattgagca gctgcaatcg tcttgccttt ggcagcatct
     6181 gcaaaagagc ctgcataagc aactcttact ttgatggtat catctactga tttcacacca
     6241 gcttcaaaac ctttttcaaa gcgcttgaca acatctcctt ccataccacc tacaaaacca
     6301 acttgcttgg tttttgtcgt tttagctgct gcaacaccgg ctaggtatgc cgcttcatgg
     6361 tctgaaaagg tgatacttgc aacatttttt tgacctttaa tcacatcatc cacaattgca
     6421 aaatggttgt caggattgtt tgcggctact ttttctacag cgtcatgtaa tggaaatcca
     6481 atcccaaaaa caagattata accattggtt actgctgact caaggtttgt ggtaaactct
     6541 gattcattgg ctgattggaa ataatcatag ccttttcctt tttcaagttt attttctttt
     6601 ccccaagctt gtaagccttc ccaggctgac tggttaaatg acttgtcatc aatacccgtc
     6661 tgattagtga tcatagcgat ttttaatgaa ttgtcagttg tttgtttaga ggagttattg
     6721 gtgcagcctc ctaatgtgaa tagggcagtc gaaacaagac caagtgacat tacttttttg
     6781 ttcatgtgaa aatgaacctc ctaaatgttt aatcctaaag tgatgtgtca ttgttaacgt
     6841 tctacttatg ctctagaacg aaaagtttag tatatcaaaa aaatcagttt tatcctacta
     6901 tttttgctga aaaatctctt tttcaccaaa atgaccgaac gagaaagtga gatcaactgg
     6961 taaaacccgt tatctcctca aaaatcacct cttaaggaga taagacaacg acaaaactca
     7021 agctactagt aatagcatga ctaactatac aatccaacaa gcggtaaaaa gagctggaaa
     7081 cgctatccaa ctctttgtca tactagacac cttagataga tgcccagact gactcaagaa
     7141 tattggtctg ttcacgccct ggaccaactg agaaggttga aatgcgaacg ccaaccaatt
     7201 ctccaacacg acgaacgtag ttgcgggcat tttcaggaag ctcatcaagg ctacgaacac
     7261 ctgtgatgtc ctcttgccag cctggtaatt cttcatagat tggtttgcaa cgtttgagtt
     7321 gttcaaggct tgctgggtaa tagtcaatac gtttcccatc aaggtcataa gccacacaaa
     7381 tcttaaccgt atcaagccct gaaagaacat caattgaatt cagagagagg ttagtaatcc
     7441 ctgacacacg acgactatgg cgcataacaa ctgagtcaaa ccaaccgaca cgacgtggac
     7501 ggccggtcgt tgtcccgtac tcatgaccca cttcacgaat gcgctcaccc acttcatcaa
     7561 agagttctgt agggaatggc ccatcaccga cacggcttgt gtaggcttta cagacaccaa
     7621 ctactttgtt gattttactt gggccaacac cagaaccaat ggttacacca ccagcaactg
     7681 ggtttgaaga cgttacaaat gggtaagttc cttggtcaat atcaagcata accccttgag
     7741 caccttcaaa aagaacacgt ttaccagcat caagtgcatc atttaaaatc acagaagtat
     7801 ctgtcacata ttgcttgatt tcttgaccgt aagcatagta ctcctcaaag atagcatcaa
     7861 aaccaagagg tgtactgtca tacatttttt caaataaacg gtttttctca gctaaattaa
     7921 tgcgtaggcg ctctgcaaag atatccttat ccagtaaatc tgcgatacgg ataccaactc
     7981 gagcagcttt atccatataa gctggaccaa tccctttaat agttgttccg attttattgt
     8041 caccttttgc atcttcttgc agttgatcca gctggatatg ataaggtaaa ataacatgag
     8101 ctcggtctga aatgcgaagg ttatctgtcg tcactccttc atcatgcagg tacgccaact
     8161 ctttgaccaa agatttgggg ttgaccacaa cgccattacc aataacggaa attttttgcg
     8221 ggaagaaaat tcctgatggg atcaagtgaa gtttaaactt cttaccatca atcacgatcg
     8281 tatgaccagc attatcacca ccttgatagc gagcaattac ttctgcatcc gctgacaaga
     8341 aatcagtgat tttaccttta ccttcgtcac cccactgggt accaacaaca actacagatg
     8401 tcatatcatt gtcctaactt tctgaaacac ttattctcaa taaaaatcat aggcaaacta
     8461 accactgctt aaaaaccaaa aagaaggtca gatctgtaag atcagcaatg tagctcttga
     8521 tatttaaaga aaacaggcag gaatctcacc tgctagcata taccttacaa cttattatag
     8581 caaaaaatga ttttttctgc tagatttctt agcattatta aggaaaaaga gaaactcttt
     8641 ttttaaccat gataaacaat cctaaaaaaa cgaacattat aagagaaaag cttttaacct
     8701 cgtttctcct aagctttagc agggttaaac gattctctta aaacattagc tcttttcttt
     8761 cagaaatagc ggttttaagg taaaataata gtaagttacc tctatgagat agccagtaaa
     8821 aacatatcga ggaaatatca catgattaga aagtatgacc gcacgagcac aaaaaagaaa
     8881 tcactaaact ggatttggct aatcattgct ttttttatga tttcaagctt tataggtggc
     8941 tcttctttta ctgagtcact tctagacatt ttgcctgcca tagccattgg tggtacaggt
     9001 tatgctattt ttcgtgttag atctcatcaa aagcggctag caaaggccaa gattgccaaa
     9061 caattagaag atctaaaagc aaaaattcag ctagctgatc gtaaagttcg gctgttagac
     9121 acttatctgg cagaccatga tgacttccaa tataacgtac ttgctcagca attattacca
     9181 caacttagtg acatcaaagc aaaagctatt acgttaaaag accagttaga tcccaaaatt
     9241 tatcgtcgta tcaccaaaaa agccaatgac gttgaaagtg atattaccct tcaactcgaa
     9301 acactgcaga ttgccacaac tcttaaccct cagcctttga aaaccccatc tcctaatctt
     9361 attaacaaag cacctgagct caaaccgtat tatgataata ttcagacaga tcatcaagct
     9421 atccttgcta aaatccaagg agccgacaat caagaagaat tgcttgcgct gcatgaagct
     9481 aacatgagaa ggtttgagga tatcttaaca ggctacttaa aaatcaaaga agagcctaaa
     9541 aattactata acgctgctgc tcgtttagaa caagccaaac aagctatcca acaatttgat
     9601 gaagatcttg acgaaaccct tcgtcgactc aatgaaagtg atcttaaaga ttttgatatt
     9661 agccttcgaa tcatgcaagg tgctactcaa agacgaacaa cgcatcacca aaaagactaa
     9721 aggagtccct ttatggcaga ttttaatttt gacattgacc aaattgctga caatgctgtt
     9781 gtcaaaaccg ataaaaccac cgatattatc tcagacttac caactgacac aaatggccaa
     9841 atctcttttt ttgagaaact aagtgctgac cagcagacag ctattactgc caaagccccg
     9901 gcattggtag ataccttttt ggcagaccaa aatgcgctac ttgattttgg tcagtctgct
     9961 gttgaagggg tcaatgcaac ggttaatcat atcttggcag agcaaaaaaa attacagatt
    10021 cctcaggttg atgatctttt aaaatcaacc aatcgtgaac tcaatggttt tatcgctaaa
    10081 tacaaggatg ccacacctgt ggaccttgat aaaaagccaa actttcttca aaaactcttt
    10141 aaacaaagcc gtgataccct gcaagagttt tattttgatt ctcaaaacat tgagcaaaaa
    10201 atggatagta tggctgcagc ggttgtcaag caagaagata ccttagctcg caatattgtc
    10261 tcagcagaat tgttaattga agataatact aagtccattg aacatttggt aggcgtcatt
    10321 gcttttatcg aagctagtca aaaagaagct agtcagcgcg ctgcggcttt acaaaaagac
    10381 ctaaaaacca aagacagtgc tacaccagac tatcaaataa aagctgatct tttggcaaga
    10441 actaccgaag tcatcaacac cctcgaacaa caacacaccg aatacttgag tcgcctctat
    10501 gttgcttggg cgacgactcc gcaaatgcgt aatctagtca aggtctcttc tgacatgcgt
    10561 caaaaactag gcatgttacg ccgcaatacc attccaacga tgaaactatc tattgctcag
    10621 ttaggcatga tgcaacaatc ggttaaatct ggaatgactg ctgacgccat tattaatgct
    10681 aataatgctg cccttcagat gttggcggaa accagcaaag aagccatccc agcattggaa
    10741 caatcagcac aaaaccctac tttgtctatg aaatctgtga caagtctggc agaaagttta
    10801 gtagcacaaa ataatgggat cattgcggcg attgatcatg gacgcaaaga acgagcacaa
    10861 ctagaaagtg ctattatcag gtctgccgaa accattaacg attctgtcaa actacgtgac
    10921 caaaatattg tgcaagcact tcttagtgaa ggcaaggaaa ctcaaaaaac aattgataaa
    10981 acaactcacg cataagctaa acatcacctg tcacgctact taaaaagcaa gccatcaatt
    11041 aggttaatcc ctttgatgtc ttgctttttt actacttaac gaataatcgc cttgtttgat
    11101 ataacactta tcttttatga tgttattttc ataatacgaa caaggctagc acgctcggcc
    11161 tcttctaatt tcaactcaat gtagtgaatc gtctcttcca gttgcgggat aatagaatac
    11221 tctaagccat tgacgcgacg tctagtcttt tcaatgtcat cagccattag ttgacaagtt
    11281 ttttcaactt cagctaaccg taagagcttt tctaatagtt cttttgtctt ttggatagta
    11341 ttgtccatct cactatttga gctgaggtaa ctataagcaa actcaccttg ttcttgctct
    11401 cttgccgtat tagactggac atggaattta ggcacattga cactcatgat attttcaata
    11461 tcaatccata aggtcacttc atggactgga acagcaaaga gttcttctac cattagacta
    11521 ttttcggatg cttttgccaa gacaaactcc ttcatattgg cagcaagttc tttttcgata
    11581 gtttgacgaa gctcattgtt ttcacgaatt aaatcagcga agcgacgcat caactcatcc
    11641 cgcttgtcct ttaataattt atgacctctt gtagctgtct tcaagcgatt tttcaagtta
    11701 ctaagttcca tgcgtgttgg tttaacattt aatcgtgcca ttgatataac tcctttcgtc
    11761 aagggcttga aattgcatgg tcaactcaat catttgcaac aaaaaccttt gtcatcgtgg
    11821 tatctgcttt aggcaaataa cgatctaaca tatcatcttt aatgcgtttt aattcggtac
    11881 gtggcaaaat cgacaacaat tcccaaccca agtcaagact ttcttcaatg ctacggttgg
    11941 tgtaaaatcc ttggttaatg tattcttctt caaaccggtt ggtaaaggcc acatatagtt
    12001 tatccgtttc agaaagggct gattcaccga gcacaacagc caattcctta gcttgttttc
    12061 cttgggcata ggctgcaaag agctgattca ttgtggcagc atggtcttgt cgagtcttac
    12121 cttcacctga gcctttatct tttaggcgag atagcgatgg caagacatta ataggcggtc
    12181 taaaaccatt tttatatagt tcttgcgaca aaataatctg accttcggta atataacctg
    12241 tcaaatcggg gatagggtgg gtaatgtcat cttctggcat ggttaaaata ggaatttgag
    12301 tcactgaccc ttttttcccg atcagacgac cagcccgttc atataaggtg gagagattag
    12361 tatagagata gccaggataa cctcgtctgc ctggaacttc tctgcgagcg gctgatactt
    12421 ctcgtaaggc ttcacaataa tttgtcatat ctgtcatgat gactaaaaca tgcatacctt
    12481 tttcataagc caagtattcg gctgttgtca gagctatacg tggtgttgca atgcgttcaa
    12541 tagcagggtc attagccaaa ttgataaata aaacagatcg atcaatggca cccgtttcac
    12601 gcaagtcatt cataaagaat tctgcctctt caaaagtaat acccatagct gcaaacacaa
    12661 ccgcaaagtt atcatcagag tttagaacag ttgcctgacg agcaatctgt gcggccaatt
    12721 cattatgagg taaccctgag cccgaaaaaa ctggtaactt ttgaccacga acgagggtat
    12781 tgaggtgatc aatagctgag atacctgtct ggataaattc gtctgggtaa tcacgagcga
    12841 caggattgat cgcttgacca tcgatatcaa ggtatttttc gggaataagt tctggaccgc
    12901 cgtctatggg ctgccccatt ccatcaaaga tgcgccctac catatcttct gatacggcta
    12961 attccaaagg atgccctgta aatcgaacct tggttttggc taggttgatg ccagttgatc
    13021 cttcaaatag ctgtaccata gccttgtcac cttggacttc taagacttga cctttacgcc
    13081 tttctccatt atgcaaagta atatctacca attcgttgta gtgcactcct gcaacttgat
    13141 caacaatcat caaagggcca accacttcgc taaccgtccg atattctttg agaacgctca
    13201 tctaaattcc tccctttgct aaaacgtggt gaatctcttt ggtaaccttc tgagtaagcc
    13261 ctttaatctg ctctaagttt tcttctggga taaatttgct gcgtgcgatg cgatcgcgta
    13321 cctgagcagt gccttccata atctccttaa aataagctcc caaagaaagg gctttgctgg
    13381 cttcctcatt aaaggtcaaa atattggtta gcatggcctc ttgtttcgga aaggaagtaa
    13441 aggtatccac cgaatcaaag gcattttgct ggagataatc ctcccgaatt tgccgggcaa
    13501 cagccatggt caaacgatct tgttcagaca gtgaatcaag ccccaccaag cgtacaattt
    13561 cttctagact ggcttcacgc tgcaaaatag ccatggcacg agttaccttg ttggaccaat
    13621 tagattcctg tttacggtca atatagcttc ctacatcatc ttgatacaat gaataagacg
    13681 tcagccagtt aatcgctggg aagtgacgcc gttgcgcaag aggcgcgtcg agcccccaga
    13741 aaactttgac aatccgaagg gtgttttgag tgacaggctc tgaaatatct cctccaggag
    13801 gagaaaccgc gccgatggct gtaatggttc cctcacgttc ttgacttccc aaggtccgaa
    13861 cacgaccagc ccgttcataa tattcggcaa tacgactccc taagtaagcc ggatagcctt
    13921 catcaccagg catttcttgt aggcgccctg acatctcgcg cagagcttct gcccaacgtg
    13981 atgtcgagtc tgccatgata gccacagaat agcccatatc acggaaatat tcggcaatgg
    14041 taatgcctgt gtaaatcgac gcttcacgcg ctgctactgg catattagag gtgtttgcaa
    14101 ttaacaccgt gcgctccata atggactggc ctgtatttgg gtcaattaac tctggaaact
    14161 cattcaaaac gtcggtcatc tcgttgccgc gttccccaca accgacataa ataacaatat
    14221 caacgttggc aaatttagct atttgatgct gcacaactgt ttttcctgcc ccaaatggtc
    14281 caggaacggc agcggcacca ccttttgtaa cagggaaaaa ggtgtcaata acccgttgac
    14341 ctgtaaccaa aggctcgact gggataagct tttgagcaac agggcgactt tgacgaactg
    14401 gccaagtctg catgaggcta ccttggtaaa tggaaccgtc ttcctgctta atttcataaa
    14461 ctgtatctgt tactgtgaag ttccctgcat gaatagccac caaggtccct gaaactttat
    14521 aaggaaccat gatacgatgc tcaatgacag ctgtttcttg cacagttcct aaaatatcac
    14581 ctgcaactac ttcttgacca acacttagct tgggaataaa tgcccactta gcctttcgat
    14641 caagacttgg gatagccaca ccacggatta aaaagtcgct gtccgttgct ttttgaaaac
    14701 gatcaagcgg tcgctgaata ccgtcaaaca tttctgaaat caggcctggg cctaactcga
    14761 ccgacaaagg acaaccagta gtcactactg gttctcctgg accgatccct gatgtttcct
    14821 cataaacctg aatagacgct tgatcgcggc gcatttcaat aatttctccg actaagccaa
    14881 gatgtcccac acgacaaata tcttgaatat tagcttcttg catcccagaa gccacaacaa
    14941 gaggtcccga aacggttatt atttttcctt ggttcacctt tttcctcctt tattttttcc
    15001 aaatcatact aactaatcta tactgtcaag gtttaatgtg actattttga atagtcagca
    15061 aactatccta tgcgttcagc tgctaacttc accaattttc ctttccttgc atttcatcag
    15121 tgagctactc ataagcctca aacagagtat aagtcctctt tgcctaacgt cttagtgtta
    15181 taaaatattt tgaccgacag ccttttcaac catggcttgt atgcgtgaca ggcctatccc
    15241 ttgcgctcct tgatgagttg gtaatggtat gactgctggt agaacttgat tgtcatagtg
    15301 agtgagggct tctggaatag ctgcagcaat atcttctgtg atataaataa taccaaagtc
    15361 ttccattgct agttgacgca actggttaat agcgtcttgt ggttttatta ctggaaaagt
    15421 ttgaaaccca atcatctgaa aaggtagaat aacatctctg ttaccaatta ccccgacttt
    15481 atacaatgtt ttggtcatgc tattggtctc atcctttcta tgacacgttc tttgggtaaa
    15541 tcattagcta aagcagccac taaaagccgt aggtttttta cttcaaacga tttagctagc
    15601 aaaaaacgag caagcacata aggtccatcg acttgatatt tggccttcgc aaataaatgg
    15661 taaaggcatt catcaacaag gtattctaac tcagtcgttt gaagagtgcc ttggcgaagt
    15721 ttctctgaat aaggttttag ggctgtcata tagctatcag gtgttacatt ttcaaaccac
    15781 gtcatcagat ccttatcatc ttcaagtgtg ataaatgttt tacttggtct acttgcttca
    15841 tcagataata actgcatcat gaaacttatt ggtttatttt gcgatttggc ccgtctgacg
    15901 gtaatcagat tatacaaatc aatcacaata agaacagctt gttggaaaac agggtcctct
    15961 aatctttgtg ctatttgttt caacgcctta aaataagcta agtcagtacc aatttctaag
    16021 acgcgtatgt cttgataatc ttgatattcg gcccaaatgg actggatttc tgttaccacc
    16081 tcttttggaa actcatcaga agtcatcgtc ctaatcaaat gttctaaagc caccaaaggc
    16141 ttgtcaccaa ttggtagtaa taagtgactc agatctgctt gactagcttt agcttttaac
    16201 aacactttga cattatgata ggtgtaacgc aaggtaaata attgtactat gtcaggttga
    16261 ggggtttctg caaaagccca gcgataggtc ttggttagct cagccattaa gatagattct
    16321 agccgatcca aatcgttgag atcgtcaaca gttaggtgat agactgactg gtgcaacagt
    16381 cgagctaagg ttgttgtatt gggagcctgc aatagcttat caaattgctc tttagtcaat
    16441 aactcttttt ctttgacact gatagtagtg tttaattcta gaaatgttgg catcgctagt
    16501 caaacctcct atatctctga aaaaagcata tcaatgatgc gggagctttc ttctttaaga
    16561 actgattcta aaaggtaacg gtataaataa ttgtcatcga cttggtctaa actgactaaa
    16621 aatcctgctt cctgaggaat tagctcttga ttatagctta attgaggaaa cgctaatctc
    16681 agctcagcac aatcataaga actgaacttt tgccctgttt tttcgccaaa agtaacttgc
    16741 aatggctgtt ctggatattt agacaagact tgcttcaaaa aagccaattc ttcttcctta
    16801 gaaaagttcg tcatcttctc caaagaagct tcaaatagcc cctttatact gtcttgtttt
    16861 aatgctagta gagcttgtct ttcttggttt ttgagttgct gctgcgcaac ctgaaattgc
    16921 tgttcaagag cttttaactc tttttgacgc tttgctttta aatcgtgtag tccttgctgt
    16981 tgacgctctt taaagtcagt atcgagacta tctgtcgcta tctttaggca ctgttgacct
    17041 tcttgatgtg ctttttcaag aacgttttgt cttaactgtg ttatatcatt cacctaattt
    17101 cccccttttg atagtgttag tggtgtcacc gttttttaaa atctagataa ggaccgttac
    17161 cttatccaat tttatttaag agaataaagg agacgacaaa ggcaagaata gcataggttt
    17221 caaccatagc tgctaaaatg acacccttca taaagtcttt agggcgctta gccaagattt
    17281 gcataccagc tacagaaaca ttgccttggt gcttggccga aaaataacca acaatagcaa
    17341 ttggaagaga tatcaaaaag taagctaatc cttggttaac tgaaagttct ggtgtcagtt
    17401 tcatccaaat taaaatacca atagcaaagc cgtagattcc ttgactaccg ggtaacagtt
    17461 gcaagataag cgcagacgtg aatttttcgg gttcttcttt taagagcgca gctgccgctt
    17521 gaccaccttt accgacacca taagctgagc ccatgccact aagagctacg gctaaaacaa
    17581 ttcctaaggc tgcgaagaaa acgccaccat gtgctgtaaa ataatgtgct aaatgttcca
    17641 tgttaattgt cctccaaatc tgtttcttca ttgacattga cataattatc agctaacttt
    17701 aaaggattga aagcttttcc tccaccttca taaaattttc caaaaaactc tacgaaaata
    17761 agacgagcac catgcacata tcctgaaagc attgataaga aaatattgat cgcatgcaaa
    17821 agaataaaaa taaagatacc tactgtaaag cgcgtgactg gtggaaaaat cccaacaatc
    17881 atgttaaaag ccgctccaat actagctcca gaaagcccta atgccattag acgagtaaag
    17941 ctaaccaaat cacttaagta agagctaatg ccatataaat tatataatcc actacctaga
    18001 cctaaaaggc ttttgctttt tagtaaagaa acaaccaaaa tcccaaaggc attgcccaat
    18061 gcaagccact taccaataac cgataagtaa gcaaggccag atacattctt accaagaata
    18121 aataacagca ggcctaataa aataaaagtc caagctagac ttgacgtata ggccgtagca
    18181 tatgctttca tccttacttg ttgccatgct cctaacaaaa gcccaaatat aagtgtaaca
    18241 aacccaaata gcaaagaaac aaccaaaata gtaatcacat ccgttttagt tgataataat
    18301 gccacaggta agtcaaaacc aaagaaagaa ccgtaaataa gcccccatat ggctactgaa
    18361 atagctaaaa tattaaagaa ggtcactaac cgtttactgg ttttttggag attaaaaaag
    18421 accaatgcag ctagtgtcac agcatatagc aacaaaccat agcccaaatc agctaccatc
    18481 atgccaaaaa aagtaaggta taaaggtgct aaaaaaggtg tcggatcttt ttcttgatat
    18541 tttggcaaag cgtacatttc tgtaaccaat tcaaacggtg caatgtagcg gtgattacgt
    18601 agcttaatgg gaacatcttc ccaatcgtct ggcgtgacat cgtaactatc cagataaaac
    18661 atatcaccta gtgtcttagt cattagccct tttaattggt taaccgaatc agcttctatc
    18721 cacccttcta aggctatcag atgtcttgta ccgagtaatt gctttttagt ttgctgccgt
    18781 tgatactgac taaggacata gtctatttca acctgtaact gtgccagtat cttttgagac
    18841 tgcctaagct cttttagtag actatctttt tcagctagcc aattcgttag taactctttg
    18901 tttatacgaa gttgttctga tggcagtagc tgttctttat ataataaggg tttaaactga
    18961 tatttctgta aatgaatcgt atcttgtgct tgccaaaata agacaaggcc gtactcaaat
    19021 tctgtatggt atacttcctc aataaaaaga cctttgtgct gctttagctg atgataaaaa
    19081 taatcatttg ctgtactagg aatcgttcca actcttgccg acaaaaaatg aaattgcgct
    19141 aagtcctgag gcaaaacagt caaggaacgc cacttttcta actcctgaca gtattctgtt
    19201 agctggtcta ttttgttttg cagctgctct agtcgctcta tcttttgtga catcattttc
    19261 aaagccgttt gagctgcttg ttgtcgatgg ctttcttcga actgtttaaa cgaaagagat
    19321 ggcgtttttt gtctcaaggc tgttaacttt cctattggtg gtaaaaacgc tgataagcta
    19381 gcaaggccgt tttctaactc ttgttggctt tgtaaaaggt attgtaaagc ttcatcatct
    19441 agtagaggtt tttgatgcgt cagatcgtat tggacgattt gtggcaactt gacgttgcct
    19501 ttgttaaaag catcttgcca atgttttagt tgcttcatgt cacgaacttc aactaattga
    19561 gattgttgaa gtgtttttaa aacaagatca agataatctt tttcaaaaac cattgccagc
    19621 tttttcattt gactaatggc catatgtctc caccacctta tccactaact ccctaacaac
    19681 ggttagctta tgctcttcta aaatggcatg aagggctagc tgttgcgtcg ctaacctctt
    19741 ttttgaagct ttttgttctt gacgattagc ttcaaaaaac acctcacgtt tagcttctaa
    19801 tagtagctga gtttcttgtt cgtactcaag gaacagctgc tttaactctt cttctttttt
    19861 atcagctaga gattttttct tagcatcgta actgtcataa attgttctag cctcttgttc
    19921 gatactattc attagtgaca atagggtatt catcacatat caactcctta tctttatcta
    19981 ttgctaattc tttgactcac taggtactta aaaacaaagc tacaagaaag gctacttttg
    20041 ggggttaatc aaccagcgac tataagctag agctaaatct ctaacctatc tactaacagc
    20101 ttttttgaga taggataatt gcttgattaa acgaacagca tctgtaagtt aagcgctttc
    20161 aattctatcc ataaaaacaa tggcttagtt cagaaaaagg ttagctaaaa acctgtcaaa
    20221 gcagtaagat gacacagaac tctaaagctt tgtattgctg tctttcgtgt gttatcttat
    20281 acttctttag gcatttagta accttgatct agttgacggt aaggaacctt taccagccaa
    20341 tagccaagcc aatgtgataa cagtataact gctatcgtgt taaaagtcct gtcaaaaaga
    20401 tgtttattag tttctcgctt gtaattcttg ttttgttact aaacggtaat ttttaaagac
    20461 agaacctcca atataaccca caattaagct gataataata catccaaggg ctgttatcag
    20521 cactttagcg acaggattat aggcaaacat taccgcaaaa ccggcaatcg gagtagctgt
    20581 ccctggcgta tcattaacca atttcattag ggcgatcaaa acaccacatg cagcaccacc
    20641 aacaaaatta gtcacataaa tcggaatggg attggctgac gtcacatctg cctgagtcaa
    20701 cggttcaata gcaaaagcaa tattgtcttt tctttcaccc aattttaatc ggtaaaaaag
    20761 aacaccattc ataaaagaag agccaaagac agataaggca cctatagcca tcggaatacc
    20821 tgttagacca agcattgctg tcaaagccat tgatgatagc ggagctgttg ctacaacaac
    20881 aatcgtaccg cctagtatca ttcccatgat aatagggttg ctattagcac ttgaggtcag
    20941 gatgtctcca atagtcaata aagtactatt gatcactggc gtaattaact gcgccaagaa
    21001 acgagttaat ggtgcaccga caataataat cgagattaaa tcaagcccac caggaatatt
    21061 tttttccatc cacttgatta aaaaagatac cacataccca gccacaaaac ctgggataat
    21121 acctgttcct gaaactgaca aaccaatcaa aacggcataa cctggagaca caccaattgc
    21181 aatggcaacc aagatagcag cagctacccc tgataatgag ccatttgctc cgcctacttc
    21241 acttagaaac tgaaagccta aaaccttacc aaaaaaagaa tcttgaaagg cttcaactaa
    21301 aaaagaggca caagctgccg aagctaaagc tcccattgcc ttggcaccat gaggagcctt
    21361 atagttaaac aagctaaaaa tagctaaaac aagaatcaaa agacttgttc caataataat
    21421 atccataatc tattccacca atctatctca ttacataaga cactataact gcttcactct
    21481 ggcaacagct tcgacttcaa tcaaagcttg caaaggaagc gccgacactt gaaccgctgt
    21541 ccgaacaggg taaggctctt caaacaaatc agtcatgaca tgatttaata tttccacatt
    21601 ggtcacatct gttaaataca catttagctt atagatatga ttcaagtcca acttttgctc
    21661 tgccaaaata gattgtaaat taacaaaaac ctggcgacac tgtgcctcaa aaccatctga
    21721 caattgacct gtcactggat taaggggtaa ttggcctgcg gtgtatagaa aatgtccttc
    21781 aatcgtataa gttgaataag ggcccattgg ttctgggtag ctcttcatat acgaacctcc
    21841 tcattgataa tatagttaaa ttttacagga aaccgcattc acagtcaata ataaattctc
    21901 tttttgataa aaaattatta ttaaggtgat tggcaattgt tttttagtct aaaatgtatt
    21961 actatatttg taaataagaa gatagatata ggaagtttga catggataaa gaaacgctaa
    22021 actactggaa aacggttatc acatttcttc acgatgtctt aggtgataac tatgaaatta
    22081 ttttacatgt gattgataaa aatgatatct atattggaga actcgttaat agtcatatca
    22141 gtggacggtc taaacagtct cctctcacca cctttgctct tgatttgatt accaataaag
    22201 tctataagga aaaagatttt gtgaccaatt acaaagcaat cgttagtcct cagcacaaag
    22261 aagttcgggg atctactttt tttattaaag ataaaaaggg taatctcgag ggaatgctat
    22321 gtattaactt agatatttca gcctatcaag gagtagctag ggacctctta aaacttgtca
    22381 atctcaattt agaacatttc atcccaactg ctaaagaacc taaaacagtc acaccccagc
    22441 cagaagaagc tgttgaaatt ttaacctcta acatccaaga tattatcggt caaattattg
    22501 atccctcgtt actgagacac aatgtacatc ttagccaaga tgtcaaaata gacattgttg
    22561 ccaagcttta tgaaaaagga gtctttcaac taaaaggagc cgtctcaaaa gtcgctgata
    22621 ttctatgtat ttccgaacca agtgtttacc gctaccttaa aaaaattgaa gccgatcaat
    22681 aaccaaatca ccataaaacg ccagttaaaa gtatttcaga taagcatcac aatctctgat
    22741 ataccgatga ctgacgtttt ttttactcaa tatgccttta tggcaagaaa aggctactgg
    22801 tcattaacct tcagctatgc ttagaggtac acctgtcgca gcttgaacct catcaaaagt
    22861 aaacccttca ctgatttcaa ttacttggat accatctggc gttactttaa acacacccat
    22921 ttctgtgata ataaggtcaa ccacaccttt tgctgttaaa ggtaaggtac attcttttag
    22981 gagcttatgc tttcctttat tggtatgctc catagctacg atgacttttt tagcaccaac
    23041 taataaatcc atcgctcctc ccataccagg aaccattttg cctggaatca ggtaattggc
    23101 gatatctcca ttttctgcaa tttctaaagc acccaagacg gtcaaatcaa catgaccacc
    23161 acgtataatg ccaaaagaat ctgcactatt aaagaaagcg gctcctggat aaaccgtcac
    23221 aggctgtcct cctgcattaa cgatagtcga attcggatca tctgacttag gacctagtcc
    23281 aataaaccca ttttcagatt gaaaatacac tgtcatttct tcaggcacaa aattcgctac
    23341 tttggttggt agaccaatac ctaaatttac caaagtacca tcttctaatt ctttggccac
    23401 acgctttgca atacgatttt gaatctcttc tttacttaat gcaaccgcca taactatact
    23461 ccttctttaa caatgtaatc tacaaaaatg tgtggtgtat gaacgcattc tggttgtatg
    23521 gtgccgactg gtactatttc tcgcgcctga acaattgttg tttttgcaca agctgccatc
    23581 aattgattaa aattattttc agaaccacta tactgtaaat ttcccatttc atcagcttta
    23641 ttagcaaata tcaaggcgac atctgcttca aatgggtatt ctaagagata agtttttccc
    23701 ttgatggttt ttgtttcctt gccttctgcc actaagtttc ctacccctgt tggcgtgtaa
    23761 aagccaccaa tgccataagc tccgatgcga attttttcgg cgaaggttcc ttgaggaatg
    23821 agctcaatcg tcgtttcacc ggcagtcatt tggcgcccag cttctttatt taatccaata
    23881 tgtgttgcat agatggtttt gaattgatga ttagccacca tttttccaac accacgatca
    23941 acaaaaccag catcattaca gatcaaggtt aagtcttttg tgcctttgtc aaccagtgca
    24001 tcaattaacg cttctggtgt tccattagcc ataaaaccac cgaccataat cgtgtcacca
    24061 tctttaatat gactgatagc ttctgctatt gctattcttt tattctcttt catacggtta
    24121 ccttctatct tgttacgatg atactttgac cttggccacc accaatacaa agcgttgcta
    24181 atccatgtct cacatcacgc ttagccattt ctgctaataa ggtgactaaa atacgagcac
    24241 ctgatgctcc aatcgggtga cccaaagcaa tagctcctcc attgacatta actttttcat
    24301 tgtcaaaacc aaggtctcgt gaaaccgcta aagcctgagc agcaaaagct tcgtttgctt
    24361 caatcaaatc aatgtcatca attgtcagct gagcctttgc aagagccttt ttcgtagcag
    24421 gtattggtcc gcagcccata atacttgggt ctacacctgc acttgcataa ctagtgattt
    24481 tagctaaaat agggagccct aattcttcag ctttttcttt actcatcaat aaaattgccg
    24541 ctgctccatc attgattcct gaagcattac cagcagttac tgtcccctca tctttgataa
    24601 aagcagagcg taactttgct aacttatcca ctgtcgttcc aaatttaggg tattcatctt
    24661 gatcaacgag taaaggttca cctttacgct gaggaatagt gacaggtgca atctcttctt
    24721 tgaaacgacc tttttctatg gcaagttggg ctttacgttg gctatcagct gcaaaggcat
    24781 cttgttcttc tctggtcagt ccatactgtt gcacaatatt ttcggctgtt atacccatgt
    24841 gataacctgc aaaagcatca ctcaacccat ctttaagcat ggtatctact actttactat
    24901 ctcccatacg agatccccaa cgttggcctt gtaaaacgta aggtgcttga ctcatgtttt
    24961 cggtaccacc gactaaaaca atatcagcgt tgcccgtttg aatcttttga gcacctaact
    25021 caactgcttt taagccagaa ccacagacca tatcaatcgt aaaagcaggg gtatcataag
    25081 aaagccccgc atttagcgct acttgccgag caacattttg acccagacct gcatgtaaca
    25141 cattcccaaa gatcacttca tcaatggcat cacttttaag accggtatca gctaaaattt
    25201 ttgtaacaac tgtcaccccc aaatctacgg ctgataaaga tttaaagact cctccaaaat
    25261 ttcctatcgg ggtacgataa gcagatgtga tcaccacttc tttagtcata gaaacttctc
    25321 ctttttcatt attgaaacgc ttacattttt attatataat gtgaactata ataaacaaat
    25381 atcgtttttt tatatatgct ataactaaac gttatcgaaa gaggaaaata tgaatatttc
    25441 aagcttggct tattttattg agattgttaa ttgtgacttt aacttaacca aagcagccca
    25501 aaaaagtcat atttcgcaac ccgcccttag tagttatatc aagactatcg aaaaagaaga
    25561 aggagtcgtt ttattccagc gtcaaaaagg gcgtctagtt ggactaacct cagtcggcga
    25621 acgctttttt gaaaatgcta aggtggttat cgcatcacat gagcaattaa tggctgattt
    25681 gcggagtgaa tccaaaaaat acaaaggaga aatttcaatc ggtattcctc aactcatctc
    25741 tggcattgtt ttttctgagc tcattccaaa actcatcgaa gaaaatcccg aagttaaatt
    25801 tcatattgta gaaacgggtg cttatgaact gcaaaaaaaa ctgtttctaa aagaagtcga
    25861 ttttgcgatt ttattaacac ccactacact agatgctact attgctgata tcgacattct
    25921 cctaagagat caactctgcc tttatatgag tcacaaaacc tttcaagaga cgtttgatag
    25981 taatcctaac atctctatca aagcattaca tcgtcaaaac ttagtaactt ttgataaaac
    26041 ctttatgatt aatcatcagc ttaccacctt atttgaagaa gcaaatagtc ttcctgtagt
    26101 caaatgttat tctgcctcgt gggatttttt attaaattgc acgcgctatt cctcttacct
    26161 aacaatccta ccaaggccta ttactcattt tgctcatatg gacggtttag tggaggtcca
    26221 actgaccgaa catccaaaat gggaagttgt tctagcctct ctcaagcaca ataaaacaag
    26281 ccatttaaaa cattatatca agcacaccat cttagattat ttcacgaacc agcgcttcta
    26341 attgcttgac tcagacctta aaaaaacacc tgaaggaaac aatcccaagc gtttcatata
    26401 gttatttcct aaaatacaaa taggtaaatg atggcaatac ctgtgaccac caagacacaa
    26461 ggaatagtat aagccacaat ttgattgaat tttagtttga agtttgagag gtaagataag
    26521 gcccaaaaag gaactatcaa gtttgttgtg gcatctccta attgataagc ctgcaaaatc
    26581 ttacctaggt cattaccaac ttcgatggtt gcagggacga tatagggagc ttcaatcaca
    26641 aattttgagc ctcctgatgg taccgcaata ttcagcaaag ccgagtgaag ataagcaaag
    26701 accggccatg ttgtcccatt tgaaatagcg acaaagaaat gggaaatctc tagtcctaga
    26761 cctgtacttt gaatgattcc aaaaatacca gcgtaaaatg gaaactgaag caccaagccc
    26821 caagatgaca tgaccccatc tttgaatagg gaaccataat attctggtcc ttgttgacca
    26881 caaagaagca accctaagcc taaaaataaa aagttaaaac tattgagatc tagaccagac
    26941 aagcccacca ccgaaaaatg ataaaggcta tagctaacaa tggccccacc aacaagatac
    27001 atcactaagc gagatgcgtt catcttctca gcaggtgtgt ttcttgcaat aacaacatgt
    27061 gaagcggtat caagactatg gtgatagatc tcatcatcaa gttccatgat tttagtttca
    27121 tcttttgggt gcgccaataa agcaaagcaa agcggtacca gcatacacac cactaaaaga
    27181 ctgataaatg gaagattcaa aactgattct gtcagaggaa cactttcagg aacaacctgt
    27241 ttataagcat ctgcgacact atttcgcaaa taatctggag tagccgaata aagcaccgca
    27301 gcaccagata aaccagaagt agctggtaaa aaggtaaaaa ataaagtagc aacaaacaaa
    27361 ggtgtatgca ccttaatccc tttttgccta gcctgaacaa gcaactcttt cccaaacaca
    27421 atggcaacca tcataccaag accccagtgt aaaaagccaa agatagaacc tactacaata
    27481 gaaaaaataa aaatctgagt cctggtttga ggcagtttgg caagggactt aaacatttta
    27541 tgagcaggtg gagaactcgc tacagcattc cctgtcgcaa gtaaaatagt catctgcatg
    27601 gcaaaggaca acagccccca aaaaccattt acccagccat agataatccc gcccgtcttt
    27661 tccgtaccaa taaagggact atccgtcatc aacacagaca tagtaacgac taaaaaggtt
    27721 aaaataaaac agataaaaag cgattctggc atccatttca taaagccatc aatatagcga
    27781 tccataaacc tttttttggt tttgatgtta ttcatgttgt caattccttt cacattgtcc
    27841 acaagcactt ttgttctttt gatcagtaac ctatcgataa tattaataag cgtttcttta
    27901 gttcatttag ttatcaataa gtttaaaccc acaaaaaaca cttttgttcc ctttttatta
    27961 tgataacgtt ttcataaaca aacaactatg atttttttat tgcttccata acccttattt
    28021 a
//
LOCUS       DAUDYX010000022        27065 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.022, whole genome shotgun sequence.
ACCESSION   DAUDYX010000022 DAUDYX010000000
VERSION     DAUDYX010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27065
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.022"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            64..912
                     /locus_tag="VHD02_000937"
     CDS             64..912
                     /locus_tag="VHD02_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEQ5497555.1"
                     /translation="MEMTWTVKYITEFIATAFLIILGNGAVANVDLKGTKGHNSGWLV
                     IAFGYGLGVMMPALMFGNVSGNHINPAFTVGLAVSGLFPWAHVLQYVVAQLLGAIFGQ
                     LVVVMVYKPYFMKTENPNHVLGSFSTISSLDNGQKDSRKASYINGFLNEFVGSFVLFF
                     GALALTKNYFGVELVGKLIEAGYDQTTAATQISPYVTGSLAVAHIGIGFLVMVLVTSL
                     GGPTGPALNPARDFGPRLLHHFLPKSVLGQAKGDSKWWYAWVPVVAPILAAIVAVAAF
                     KYLYIR"
     gene            complement(1248..2144)
                     /locus_tag="VHD02_000938"
     CDS             complement(1248..2144)
                     /locus_tag="VHD02_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEQ5497556.1"
                     /translation="MNEMLILRLIQALLVSAMLFIFFMLVKHLKKNKINPFKRFWTGF
                     WIGLLTDALDTLGIGSFATTTTCFKLTKLVTDDRQLPGTMTVAHVLPVLIQSLCFIFV
                     VKVEVLTLLAMAAAAFIGAYFGTHITKNWHAPTVQRILGSLLIIAAIIMIIRIIYHPG
                     EHLSDTIHGLHGIWLFVGIGFNFIVGVLMTMGLGNYAPELIFFSLMGLSPTVAMPVMM
                     LDAAMIMTASSSQFIKANRVSWDGFAGIVSGGIIGVLLAVFFLTNLDINSLKLLVIAI
                     VFFTGGMLIRSSFTSHKQQTNR"
     gene            2442..3194
                     /locus_tag="VHD02_000939"
     CDS             2442..3194
                     /locus_tag="VHD02_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEQ5497557.1"
                     /translation="MTLMENITFKTPVLRVNDRDLNIAFYQNNLGLRLVSEENAIAIF
                     SSWGEGQECFVIEESPSVRTRAVEGPKKVNTIVIKTNQPKEIEQLLAHGAHYDALFKG
                     QNGYAFETISPEGDRFLLHAEQDIKHLQGTDLPSLEKDATFKGLTQFKFDIIVLNVIS
                     EERSKAFYRDLFSDQLPITMDFIQEEGPDLAIDPHIAWDLEILEFQVSKDYDMKVLKA
                     TLEEDGHKVYIDKKHKVLVLSDPSQIEVWFTK"
     gene            3179..4129
                     /locus_tag="VHD02_000940"
     CDS             3179..4129
                     /locus_tag="VHD02_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEQ5497558.1"
                     /translation="MVYEMTLAVIKCIENHLHKKVYKGASLALFQSGRWQEYHIGTID
                     GRRPVDANLVYDLASVSKVVGVATICNILLNNGTLALDDPLKVYYPSIADTTVTIRQL
                     LTHTSGLDPYIPNRDVLNAQQLRKALNHLTQKENKNFYYTDVNFLLLGFMLEELFSES
                     LDQIFDKTIFTPFGMYHTSFGPRPEAVPTLKGVSDGEVHDPKAKILKKHSGSAGLFST
                     LADLESFSNHYLNDPFSDCLWRNYSQQTSERSLGWNLDGDWISHTGYTGPFLMLNKKE
                     QTAAIFLTNRTYDEDDKSKWLKERQLLYNALKHDLTTPVS"
     gene            complement(4309..6636)
                     /gene="pflB"
                     /locus_tag="VHD02_000941"
     CDS             complement(4309..6636)
                     /gene="pflB"
                     /locus_tag="VHD02_000941"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEQ5497559.1"
                     /translation="MATVKTNTDVFEKAWEGFKGTDWKEKASVSRFVQANYTPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEATRFPYDTRPTSIADIPAGFIDKENELIYGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAITEIDEESIRLREEV
                     NLQYQALGEVVKLGDLYGVDVRRPAQNVKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFGRTKAYDALYSGDPTFITTSMA
                     GMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDQLPETFRRYCMKMSHKHSSI
                     QYEGVTTMAKEGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNGGY
                     DDVHRDYKVFNVVEPITSEVLEYDEVMANFEKSLDWLTDTYVDALNIIHYMTDKYNYE
                     AVQMAFLPTHQRANMGFGICGFANTVDTLSAIKYATVKTIRDENGYIYDYEVTGDFPR
                     YGEDDDRVDDIAKWLMEAYHTRLASHKLYKNAEASVSLLTITSNVAYSKQTGNSPVHR
                     GVFLNEDGTVNTSQVEFFSPGANPSNKAKGGWLQNLNSLAKLEFSHANDGISLTTQVS
                     PRALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLNDVYDKIMNGEDVIVRISGYC
                     VNTKYLTPEQKTELTQRVFHEVLSMDDAAEAISSK"
     gene            6845..7939
                     /gene="dinB"
                     /locus_tag="VHD02_000942"
     CDS             6845..7939
                     /gene="dinB"
                     /locus_tag="VHD02_000942"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEQ5497560.1"
                     /translation="MLIFPLINDTSRKIIHIDMDAFFAAVEERDNLALKGKPVVIGKD
                     PRETGGRGVVSTCNYEARKYGIHSAMSSKEAYERCPKAIFISGNYEKYRTVGEQIRRI
                     FKRYTDVVEPMSIDEAYLDVTNNKLGIKSAVKIAKLIQHDIWKEVGLTCSAGVSYNKF
                     LAKLASDFEKPHGLTLVLKEDALCFLAKLPIEKFHGVGKKSVEKLHDMGIYTGQDLLA
                     VPEMTLIDHFGRFGFDLYRKARGISNSPVKSDRIRKSIGSERTYAKLLYQETDIKAEI
                     SKNAKRVAALLQDHKKLGKTIVLKVRYADFTTLTKRVTLPELTRNAAQIEQVAGDIFD
                     SLSENPAGIRLLGVTMTNLEDKVADISLDL"
     gene            complement(8032..8514)
                     /locus_tag="VHD02_000943"
     CDS             complement(8032..8514)
                     /locus_tag="VHD02_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497561.1"
                     /translation="MISYEKVRQALKTSTIAIIILNGLGVVLSLMGFAGFFYLQSQLK
                     NEAFRAQLTTEQLAQLQSSMTPFMIFLSVLNVLAIIAIIIFCTQNLSKLKQGLTVSYI
                     PYILGLILSVIGLVNQFTTTLSMVGTILILIQAALYGFAFYKAKTLNEKGDDTDQAML
                     "
     gene            complement(8605..11058)
                     /locus_tag="VHD02_000944"
     CDS             complement(8605..11058)
                     /locus_tag="VHD02_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEQ5497562.1"
                     /translation="MEYVFTGTVDRIIFENQANFFKILLLAIEDTDSDIDDFEIIITG
                     TMADIIEGDDYTFWGELTQHPKYGQQLKLSRYQKIKPSSSGLVNYFSSDHFKGIGKKT
                     AEKIIALYGHNTIDHILEDPSKLETISGLSKANRQAFVAKLKLNYGTEQLIAGLVELG
                     LSNRFAFQAFEKYKEEALDLVKENPYQLVEDLQGFGFKMADALAENLGIESDSPKRFR
                     AALLHCLLEESVSRGDTYVQARQLLDFAITLLEDARQVECDPAAVAEQLSELIIEGKI
                     KNSDTKLFDASLYFAEEGIASNISRLLDTPLSQSFSHDTIQTTIQAVQKDFAITYDQV
                     QQEAITKALTSKVFLLTGGPGTGKTTVIRGILQAYANLHQIDLDKKDLPILLAAPTGR
                     AARRMNELTGLPSATIHRHLGLNGDNDYQAMEDYLDCDLLIVDEFSMVDTWLANQLLG
                     AINSTTQVIIVGDSDQLPSVGPGQVLSDLLKVNSLPQIALQKIFRQSQESTIVNLADQ
                     MRRGILAADFRDKKADRSYFEAQAAFIPDMIQKIVLSAIKSGIPAEEIQILAPMYKGQ
                     AGINHLNQLMQELLNPLQGQTEFLFNDTHFRKGDKVLHLVNDAQLNVFNGDIGYITDL
                     IPAKYTESKQDELILDFDGSEVTYPRNEWLKLTLAYAMSIHKSQGSEFQVVILPITRQ
                     SGRLLQRNVIYTAITRSKSKLILLGEYTAFEYAIKHEGDKRQTYLIERFQEQSDLASS
                     QPNQELKSKEQTSLFSNTATLEDDSQKSSSQSTNSNPTENSQSDNDDFRLTPENYSTI
                     DSMIGLTESDIALFFQKKS"
     gene            complement(11116..11709)
                     /gene="lepB"
                     /locus_tag="VHD02_000945"
     CDS             complement(11116..11709)
                     /gene="lepB"
                     /locus_tag="VHD02_000945"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEQ5497563.1"
                     /translation="MKQFIKEWGPFTLFLILFGLSRLFLWQAVKVDGHSMDPTLAHGE
                     RLIVFNQARIDRFDIVVAQEEENGQKKEIVKRVIGLPGDTISYNDDTLYINGKKTVEP
                     YLAEYLKQFKNDKLQKTYAYNTLFQQLAETSDAFTTNSEGQTRFEMSVPKGEYLLLGD
                     DRIVSRDSREVGSFKKENLIGEVKARFWPLNKMTVFN"
     gene            complement(11720..12622)
                     /locus_tag="VHD02_000946"
     CDS             complement(11720..12622)
                     /locus_tag="VHD02_000946"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEQ5497564.1"
                     /translation="MNTLVLKIDAILSKHLKKQLASYTISSQNTYVAFAAKKNGVTVL
                     LYKSGKLVLQGNGANALAQELNLPVAKTVFEASNNSQDIPIIGSDEVGNGSYFGGIAV
                     VASFVDPKDHSFLKKLGVDDSKKLSDKTIQQIAPLLEKQIPHQSLLLSPKKYNELVGK
                     SKPYNAISIKVALHNQAIFLLLQKGIQPKQIVIDAFTSQSNYEKHLKKEKNHFPNPLT
                     FQEKAESHYLAVAVSSIIARNLFLDNLDQLGQDLGYQLPSGAGSASDKVASQLLAAYG
                     MSSLEYSAKLHFANTHKAQALLTK"
     gene            12779..13087
                     /locus_tag="VHD02_000947"
     CDS             12779..13087
                     /locus_tag="VHD02_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497565.1"
                     /translation="MKSINRYKFTFGEKTLTLTTDKDNLFMEEVERVAKEKYQALKNH
                     LPEADDETVAILMAINTLSTQLSREIAIEKMEAEILDLRQKTLVGLQEKANQIDVNEV
                     "
     gene            13090..13635
                     /locus_tag="VHD02_000948"
     CDS             13090..13635
                     /locus_tag="VHD02_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEQ5497566.1"
                     /translation="MLSLLIVLVLTWNFYIGYSRGIILQSFYVLGALLSLLVANRFYI
                     GLAHKLTLWIPYSNPVEGTSVFFFKSVDIFVLDKVYYAGLAFFIIFLLGYALSRFLGI
                     FVHFLLLDYFDNQWTKCLSGGLALLVSLLFLNMLLSIFATVPMPFLQHYLHSSFLARL
                     VIEHLPPLTIIIQKLWIQAII"
     gene            13784..16123
                     /locus_tag="VHD02_000949"
     CDS             13784..16123
                     /locus_tag="VHD02_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEQ5497567.1"
                     /translation="MNNKILEQLEFNKVKELLLPYLKTEQSQEELLELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEEGRILHTFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPKISDDSTLALINVRHPLLSNPVANDLHFDHDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKITVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADRNSLVLFDELGAGTDPQEGASLAMAILEHLRLSHIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFTLVRVQEEQ
                     KVKSKQINVVKKADSSGPRARLDLRGKRYEEAMQELDHFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRNKHVKHFAYAPQNAGGSGATIVTLG"
     gene            16127..16627
                     /locus_tag="VHD02_000950"
     CDS             16127..16627
                     /locus_tag="VHD02_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEQ5497568.1"
                     /translation="MESYEQFYAKLSQPFRKSPQLIILLNFLLKIVTGMMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRFDCPRPYEKWNIKPLIDKDTKGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLML
                     GLCLLI"
     gene            16708..17022
                     /gene="trxA"
                     /locus_tag="VHD02_000951"
     CDS             16708..17022
                     /gene="trxA"
                     /locus_tag="VHD02_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEQ5497569.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQAPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(17074..17661)
                     /locus_tag="VHD02_000952"
     CDS             complement(17074..17661)
                     /locus_tag="VHD02_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ5497570.1"
                     /translation="MEIGQQIIRYRKQQALSQEKLAEKVYVSYQSISNWENDKTYPDI
                     HSLLLLSQIFQVSLDQLIKGDIEKMKYTITQVDKKNFKRDTKVMVTLMILLMISSYPL
                     VYFLEWLGLGIFVLLSIITMTYANRVERFKKKYDVQPYKEILAVSNGKLLDEIEKREE
                     RAKLPYQKPLIVTVFFLITVAFAFASRFMFTWLFH"
     gene            complement(17737..18861)
                     /gene="mutY"
                     /locus_tag="VHD02_000953"
     CDS             complement(17737..18861)
                     /gene="mutY"
                     /locus_tag="VHD02_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEQ5497571.1"
                     /translation="MWDNETIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YGKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFEREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFSLYPFATPQKKMLETYLKQKNA"
     gene            19059..19352
                     /locus_tag="VHD02_000954"
     CDS             19059..19352
                     /locus_tag="VHD02_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497572.1"
                     /translation="MFQKLKNLIDRYSWFVFIMMILVLFLNYIYPFDPSLSGLQLEWL
                     IMTQVFPGLGIVCAMVAIIFKKFNLFVLALILYFALWLKLFLAFSFFTYFLGV"
     gene            19525..19815
                     /locus_tag="VHD02_000955"
     CDS             19525..19815
                     /locus_tag="VHD02_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEQ5497573.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKDWE
                     KRRLAYEINDFREGLYHIVNLEATDAAALNEFDRLSKINGDILRHMIVKLDA"
     gene            19837..20328
                     /locus_tag="VHD02_000956"
     CDS             19837..20328
                     /locus_tag="VHD02_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEQ5497574.1"
                     /translation="MINNVVLVGRMTKDAELRYTPSQVAVATFTLAVNRTFKSQNGER
                     EADFINCVIWRQPAENLANWAKKGALIGVTGRIQTRNYENQQGQRVYVTEVVADNFQM
                     LESRATREGGSTGSFNGGFNNNTSSSNSYSAPAQQTPNFGRDDSPFGNSNPMDISDDD
                     LPF"
     gene            20493..20732
                     /locus_tag="VHD02_000957"
     CDS             20493..20732
                     /locus_tag="VHD02_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEQ5497575.1"
                     /translation="MAQQRRGGFKRRKKVDFIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            complement(20865..21521)
                     /locus_tag="VHD02_000958"
     CDS             complement(20865..21521)
                     /locus_tag="VHD02_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEQ5497576.1"
                     /translation="MELQELTKKNQEFIHTATNKLIQDGKSDEDIKLILEEAIPAILE
                     NQKKGVTARNLLGTPTAWAASFSQDPSQRAAETDKNTNPWLMWLDTSLLFIGIVALLN
                     GIMTFFNTNATVTGLISLLALGFGGGASMYATYYFIYRHLGKDKSLRPSWFKIIAALS
                     LAMLIWIALYSATAFLPTSLNPQLPPLALLIIGGVSLALRYYLQRKYNIQNTMSPVNK
                     "
     gene            complement(21653..22597)
                     /locus_tag="VHD02_000959"
     CDS             complement(21653..22597)
                     /locus_tag="VHD02_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEQ5497577.1"
                     /translation="MKQMFLSSAIEFKEIETFEPGAWIKLVNPSQEESMKIADQFNID
                     ISDLRAPLDVEETSRIAVEDDYTLIIVDVPIYEERNNKSYYITMPLGIIVTENAVITT
                     CLHDMTLFDHFHNRRVKNFYTFMKTRFVFQILYRNAELFLTALRTIDRQSERLEAQLE
                     AATRNEELIDMMELEKSIVYLKASLKFNERIVKKLSSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMAGIYENVLNAMTETTASIINNNQNTIMKTLALMTMALDIPTVIFSAYGMNF
                     QNNWLPLNGLEHAFWYITLIAMLLSSFVVIYFIRKKWF"
     gene            complement(22841..23995)
                     /locus_tag="VHD02_000960"
     CDS             complement(22841..23995)
                     /locus_tag="VHD02_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEQ5497578.1"
                     /translation="MPISYLKISTPQRKERGKTMIKKYTKKDGTTAYCLRAYLGVDPV
                     TGKQVRTTRQGFKTEREAKRAEVKLIDDFQRQGAWKSNDRTTFDDVAKLWFEQYKNTV
                     KPSTFLVNQNYYKTILKPHLGQLQMTKITVMICQKFVNCLSRYSGYTLYLSLANRIFK
                     FAVNLGIIDNNPMSKTLRSKCTYKNVNTLTKKYYTKEELNTFLRIVEAEESLEMRLIY
                     RLLSYGGFRIGELMALKDTDFEFHNNTISITKTIAYTKEGWAVQSPKTKKSTRAISMD
                     AETMSLAKLYIKQSIKPLHGSFKLFNFSCDTVRNRLDKLILKHGLKRITPHGFRHTHA
                     SLLFEAGIPAKIAQERLGHAKIAITMDLYTHLSKKSKDDVADKLAELVAV"
     gene            complement(24110..24532)
                     /locus_tag="VHD02_000961"
     CDS             complement(24110..24532)
                     /locus_tag="VHD02_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HEQ5497579.1"
                     /translation="MKKCSIIAIVVVGVMATATTPELSNKLFSQSVTVVAVSKEYQNA
                     LETAKMVKDANMSKKAFYDMMINESKFEKKAVDYAAKKLKINWKKNALAQAKSMQKFG
                     ASKETIKEQLSANSDGGGFTKKEVNYAIKHLEDNNKDK"
     gene            complement(24604..25344)
                     /locus_tag="VHD02_000962"
     CDS             complement(24604..25344)
                     /locus_tag="VHD02_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ5497580.1"
                     /translation="MSGSLGNKKIMASNIRRHLEKKNMNPKEFSKAMNFKYSTVLDWV
                     NAKTYPRIDKIELMANYFNVEKSDLVEMYSPEIISATSNIVIQLDKELKEPRHSDWIS
                     YGENLLSEQNTVEDSKNTVVELFSYNYYDHAASAGTGQYLNDVQVEKIELPVDYDADF
                     VIPVYGDSMEPKYHSGDYVFVKLSVDLTDGDIGVFEYYGDAYIKQLLINAEGAFLHSL
                     NSKYDDIPIDADSDFRIIGEVVGSYSGK"
     gene            25704..25853
                     /locus_tag="VHD02_000963"
     CDS             25704..25853
                     /locus_tag="VHD02_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497581.1"
                     /translation="MRERNEKFIGVLKDMHMQILRDFTNEEDPLTRCELAKGFLEIGD
                     YLSKQ"
     gene            complement(25839..26054)
                     /locus_tag="VHD02_000964"
     CDS             complement(25839..26054)
                     /locus_tag="VHD02_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497582.1"
                     /translation="MKISNSKDLALAIVASSSPTLSIEDKIKLYEDSVEAIKQHNLPF
                     VEAEKQEQINNGKVIAEALERGESLFG"
     gene            26113..26271
                     /locus_tag="VHD02_000965"
     CDS             26113..26271
                     /locus_tag="VHD02_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497583.1"
                     /translation="MSWKKLMLGDLEHTFTSRDGKEKTSVEFEGGVLPALLVLGGITW
                     LIAWFITK"
     gene            complement(26370..27011)
                     /locus_tag="VHD02_000966"
     CDS             complement(26370..27011)
                     /locus_tag="VHD02_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497584.1"
                     /translation="MNFFNFLLCVFKFTSEYLIKNWIALIALFLSYSNYRRNNLQVEL
                     IAAPVSDWILSVILDNGESIYNPNGTLRANIKIINPSNVDVSYFDLIVFDKNRKYQHY
                     YQKQNNIINDLTGREAIAAVQPDGNTILIEVPEADCGVLKAHSMTRMDLIIQTSEITD
                     RLFVAFKVAKKKKLFKANKAGYVNSPYQSFSASFPVELSKKPHYEDILKDLHE"
BASE COUNT         8292 a         4800 c         5340 g         8633 t
ORIGIN      
        1 tcatcaataa gcctgttaaa atggtatttg ttatatttta ttatttggag gaaaccagaa
       61 aacatggaaa tgacatggac tgtgaaatac atcacagaat ttatcgcaac ggcttttttg
      121 attattttgg ggaatggagc tgttgctaac gtcgacttaa aaggaacaaa aggacataac
      181 tcaggttggt tagtgattgc ttttggctat ggcttaggag ttatgatgcc agctttgatg
      241 tttggtaatg tatctggaaa ccatattaat cctgctttca cagttggact agcagtatca
      301 ggcttgttcc catgggctca cgtcttacaa tacgttgtgg cacaattact tggagctatc
      361 tttggtcagt tggtagtggt aatggtttat aaaccttact ttatgaaaac tgaaaatcca
      421 aatcatgttt taggctcatt ctcaacgatt tcatctcttg ataatggtca aaaagacagc
      481 cgtaaagcct cttatatcaa tggtttctta aacgaatttg taggttcatt tgttcttttc
      541 tttggtgctt tggctttaac taaaaactac tttggggtag agttagttgg taaattgatt
      601 gaagcaggct acgaccaaac aactgcagca acacaaattt caccatacgt gacaggctca
      661 ttggcggttg ctcacattgg tattggtttc ttagttatgg tcttggttac ttcattaggt
      721 ggaccaactg ggccagcact aaacccagcg cgtgattttg ggcctcgttt gttacaccac
      781 ttcttaccaa aatcagttct tggtcaagcc aaaggtgatt caaaatggtg gtatgcatgg
      841 gttcctgtag ttgcacctat cttagcggcg atagtagccg tagcagcgtt taaatacctt
      901 tatatcagat aaaaaaagaa ctaaagcttc ttttagactc aagacaagct cgcttaagga
      961 acttgtcttt tttagatgag ttaagcgaaa tctaatgcaa acttgactgt cttgtatagc
     1021 gtattttgta aattttatta gacttcttgt aaaaaagcct ctcctcagat aagatgacta
     1081 gtgcctctaa acggtgttac aaataaacga ggcatcccta acttccttta tctggtgttg
     1141 aaactatttt gcacggacta aatcactgac agagtaaaaa acgatttttc atttttgttg
     1201 taaaatatta aaaacgtctc tggttagtga ttcccagaaa cgttatctta tctatttgtt
     1261 tgttgcttat gagaggtaaa ggaggaacga atcagcattc caccagtgaa gaaaacgata
     1321 gcgatgacca gaagtttcaa gctattaata tctaaatttg ttaagaaaaa cacagctagt
     1381 aaaacgccaa taataccacc agaaacaatt ccagcaaaac catcccaact gacacggttt
     1441 gctttgataa actggctact agaagctgtc ataatcatag ctgcatctag catcataaca
     1501 ggcatagcaa ctgttgggct aagacccatt aatgaaaaga aaatcaattc tggagcataa
     1561 ttgcctaacc ccatagtcat caagactcca acgataaaat taaaaccaat tccaacaaaa
     1621 agccagatgc catggaggcc atgaatggtg tctgaaaggt gttctcctgg atggtagatt
     1681 attctaatga tcatgattat agctgctatg attaaaagac taccaaggat tcgttggaca
     1741 gttggagcat gccagttttt tgtaatatga gtcccaaaat aagctccaat aaaggctgct
     1801 gcagccatag caagtaaagt tagaacctca acttttacga caaaaataaa gcaaagagat
     1861 tgaatgagca ccggaagcac atgagctacg gtcatagtgc caggcaattg tctatcgtca
     1921 gtgactaatt tagttaactt aaagcacgtt gtggtagtag caaaagaccc tatcccaagg
     1981 gtgtcaaggg catctgttag caggccaatc caaaaccccg tccaaaagcg tttaaaagga
     2041 ttgatcttat tttttttaag atgttttact aacataaaga aaataaaaag cattgccgaa
     2101 actagcaaag cttgtatcag tctaaggata agcatttcgt tcataaagac tagtttacaa
     2161 aaaaaaacct tagccgtcaa cgtgatttac ttcattaaaa catgccacta tgcttagaag
     2221 agctttagtt cgatgtattt tgttaaaatt aagctaaaat attaatattg catgaaaggg
     2281 gacgccttcc tttttccctt actgagctta gctgttattg tagtgaatgt ttaaaaggct
     2341 agtttgtcgc tctttatcaa aaatctagta ggatgaatac gtataatttc ttgtcaaata
     2401 gaggaatttt gtctataata gcagaggatt aggaggaaat tatgacttta atggaaaata
     2461 ttacctttaa aactccagta ttacgcgtta atgatcgcga ccttaatatt gcattttatc
     2521 aaaataattt agggcttcgt ttagtatctg aagaaaatgc gattgctatc ttttcatctt
     2581 ggggagaagg gcaggaatgt tttgtaattg aagagtcacc ttcagttaga acgcgtgcgg
     2641 tagaaggccc taaaaaggtt aatacgattg tcatcaaaac aaatcaacct aaagaaatcg
     2701 aacaattatt agctcatgga gcacattatg acgctctttt taaaggtcaa aatggctatg
     2761 cctttgagac tatttcccca gaaggagacc gattcttgtt gcatgctgaa caagatatta
     2821 aacatctcca aggtactgat ttaccatcgc ttgaaaaaga tgcgactttt aaaggtttaa
     2881 ctcaatttaa gtttgatatt attgttttaa atgttattag tgaagaacgc tcaaaagctt
     2941 tttatcggga tctgtttagt gatcagttac ctattactat ggattttatt caagaagaag
     3001 gtcccgattt ggcaattgat ccccatattg cttgggattt agaaatttta gaatttcaag
     3061 tctcaaagga ctatgatatg aaggtcctaa aagcaacttt agaagaagac gggcacaagg
     3121 tctacattga taaaaaacac aaagtattgg tcctttctga tccaagtcag atcgaagtat
     3181 ggtttacgaa atgactttgg cagtcattaa atgtattgaa aaccatcttc acaaaaaagt
     3241 atacaaaggg gccagtctgg ctctctttca atctggtcga tggcaagaat accatatcgg
     3301 aacaattgat ggaagacgac cagttgatgc taacttagtt tatgacttgg ctagtgtttc
     3361 aaaagttgtt ggtgtggcca caatatgcaa tattttattg aataatggta cgttagcatt
     3421 agatgatccc ttaaaagtat attatcctag tattgctgat acgactgtca ctattcgaca
     3481 gttattgact cataccagtg gtcttgatcc ttatattcct aacagagatg ttttaaatgc
     3541 acaacaatta agaaaagcac ttaatcatct tactcaaaaa gaaaataaaa acttttatta
     3601 cacagatgtt aatttcctct tgttaggctt tatgttagaa gagctcttta gtgaatcttt
     3661 agaccagata tttgataaga ctatctttac tccctttggg atgtatcata cgtcttttgg
     3721 ccctcgtcca gaagctgtac caacattaaa gggtgtgagt gatggagaag ttcatgatcc
     3781 caaagcaaaa atcttaaaaa aacattctgg atctgcgggg ctattttcaa ctcttgcaga
     3841 tttagaaagt tttagcaatc attatcttaa tgatccattt tcagattgtc tgtggcgcaa
     3901 ctacagccaa caaacaagtg agcggtcact agggtggaat ttagatggcg attggattag
     3961 tcatacaggt tacacagggc cgtttctaat gcttaataaa aaagagcaaa cggcagctat
     4021 ttttttgacc aaccgtactt acgacgagga tgataaaagt aaatggttaa aagaacgtca
     4081 gttgctttac aatgcactaa agcatgatct gacaacaccc gtatcgtaac gtctaaaggg
     4141 gcagttttag tgatcttatt actgttttta aagtttcttc taaaaaatag ccatttcatt
     4201 tgtctgatgc gattagagtg attataagtg aaaaaagaac agccatctca ttttgaatca
     4261 aatgagatgg ctgttctttt ttataagtta ttgatcattt agatactact atttacttga
     4321 aatagcttca gcggcatcat ccattgaaag cacttcgtgg aagacacgtt gagtcaattc
     4381 agttttttgt tctggtgtaa ggtatttggt gttgacacag taaccagaga tacgcacgat
     4441 aacgtcttcg ccgttcatga ttttgtcata aacatcgtta aggtccataa cgttcaagtt
     4501 aacgtgttga ccaccatttt caaagtaacc gtcaagcact gttaccaagt tgtcaacttg
     4561 ttcgtcaaat gttttaccaa gagcacgagg tgatacttga gtagtcaatg agataccatc
     4621 gttagcatgt gagaattcca gtttagcaag tgagttaagg ttttgtaacc agccaccttt
     4681 tgctttgtta gatgggttag cacctggtga gaagaattcc acttggctag tgttaactgt
     4741 accatcttcg ttcaagaaga cgccgcggtg aactggagag ttaccagttt gtttagagta
     4801 agcaacgttt gaagtgatgg taagaagtga tactgaagcc tcagcattct tgtaaagttt
     4861 gtggcttgca agacgtgtgt ggtaagcttc catcaaccat ttcgcgatgt catcaacacg
     4921 gtcgtcatct tcaccgtaac gagggaagtc acctgttact tcgtagtcat agatgtagcc
     4981 attttcatcg cggatagttt taacagttgc gtatttaata gctgacaagg tatcaactgt
     5041 gttagcgaaa ccacagatac cgaatcccat gttagcacgt tggtgtgttg gcaagaaggc
     5101 catttgaact gcttcgtagt tgtatttatc agtcatgtag tggatgatgt taagcgcatc
     5161 tacataagta tctgtcaacc agtcaagaga tttctcaaag ttagccataa cttcgtcgta
     5221 ctcaagaact tctgatgtga ttggttcaac aacgttaaac actttgtagt ctctgtgaac
     5281 atcatcgtaa ccaccgttaa gaccagtaag aagggctttg agaacgttta cacgagcacc
     5341 gaagtattgg atgttatgac gttgttcttc attttctggg tcaagtggtg atacacaaca
     5401 tgaaatacat gacatctcac cataaccttc tttagccatt gttgtcacac cttcatattg
     5461 gatagaagag tgtttgtggc tcattttcat acagtagcga cggaatgttt ctggcagttg
     5521 gtcagtccaa agaactgtta agtttggttc tggagagtta ccgatattat caagagtgtt
     5581 taagaaacgg tagtccatct tagtcacacg gtgacgaccg tcgttaccca taccagccat
     5641 agaagttgtg atgaatgttg ggtcacctga gtaaagggca tcgtaagctt tcgtacgacc
     5701 aaattttacg gtacggagtt taaggacaaa atcatcaaca aattcttgga tttctgattc
     5761 tgtaaaggta ccgcgagcaa ggtcacgttc tgcaaagata tcaagaacga ttggcacacg
     5821 tccaagagaa gtagcagcac cgttgatcac acgacataca gccatgaaag cgatgtttac
     5881 ccattggata gcttctttaa cgttttgtgc tgggcgacga acatcaactc cgtaaaggtc
     5941 accaagttta accacttcac caagagcttg gtattgaagg ttaacttctt cgcgaagacg
     6001 aatggattct tcatcgattt cagtgatagc attccagtca ttgacttttt cttgcatcaa
     6061 gtagtcagca ccgtaaagag caagacgagc gtaaacaccg atgatacgtc cacgtgagta
     6121 agcatctgga agaccagtta cagtgtgggc atgacgagcg cgacgaatat ttgaagtata
     6181 agcgcggaag ataccgtcgt taacagttgt cacatactta gtaaagattt catgtactgc
     6241 tgggtctggt tcatagccgt tttctttaag ggtcgtttcc gccatacgga taccaccttt
     6301 tggcatgaag ttcaatttga acaattcatc gttttgaata ccataaatta attcattttc
     6361 tttatcgatg aaaccagcag gaatgtcagc aattgaagtt ggacgagtat cgtatgggaa
     6421 acgtgtagct tcatagtgtg cttttgtttc ttcgataact tttttgatgt gaagtgaacg
     6481 ttcagtagca ccagccaaga aagattcatc gccatcgtat ggggtgtagt tagcttgtac
     6541 aaaacgtgag acacttgctt tttctttcca gtcagttcct ttaaagcctt cccaagcttt
     6601 ttcgaaaaca tctgtattgg ttttaacagt tgccataaat ttctccttaa attctagtaa
     6661 caaagcgatc tgttacattt acaattctaa gtttaacaca taggaaacgt tttcccaagc
     6721 aaaattcttg acaaaattaa gattctttta taatacagat tgttgaaaca gtcacaaact
     6781 gtattagaat gtattttttt ggctttagat agcctaaaag gtataataga gaaaggaggg
     6841 acttatgctt atttttccac tgattaatga cacgtcacga aaaatcatcc atattgacat
     6901 ggatgccttt tttgctgcag ttgaggaaag ggataacctt gctttaaaag gaaagcctgt
     6961 tgtgattggg aaagatccaa gagaaacagg tggtcgcgga gttgtttcca cttgtaatta
     7021 cgaagcgaga aaatatggca ttcattcggc catgagctct aaggaagctt atgagcgttg
     7081 tcccaaagcc atttttattt caggaaatta tgaaaagtat cgaacagttg gggagcagat
     7141 ccgccgtatt tttaagcgtt atactgatgt ggtagagccc atgtccattg atgaggccta
     7201 tctagatgtc accaacaata agttgggtat aaaatcagcc gtcaaaatag ccaagctgat
     7261 tcagcatgat atctggaaag aagtaggatt gacctgttca gcaggtgtgt cttataacaa
     7321 atttttggct aaattggcta gtgattttga aaaaccccat ggcctcaccc tagtcttgaa
     7381 agaagatgcc ctgtgctttt tagccaaact ccccattgaa aagtttcatg gtgttggtaa
     7441 aaaatcagtt gaaaaactgc atgacatggg gatttataca ggacaggatt tgttggcagt
     7501 tcctgaaatg accttgattg atcattttgg tcggtttggt tttgaccttt accgtaaagc
     7561 gagaggcatc agcaattctc ctgtcaagtc tgatcggata cgcaagtcaa ttggcagtga
     7621 gagaacctac gctaaactgc tttatcaaga aacagacatc aaggcagaga tcagtaaaaa
     7681 tgctaagcgc gtggccgctc tcttacaaga ccataaaaag ttaggcaaga ccattgtgct
     7741 caaagtgcgt tatgctgatt ttaccacctt gacaaaacgt gtcaccttgc cagaattaac
     7801 cagaaatgcc gcacaaattg agcaagtagc tggggatatt tttgacagct taagcgaaaa
     7861 tcctgctggt atccgcctgt taggggtaac catgaccaat ttggaggata aggtagctga
     7921 tatttccttg gacctatagg agaaagagcg ctctatccgt ccgcctcatt ttggagaaaa
     7981 aagaagctga ttagggattt ccctaatcag cttctttggt ccattatttt attacagcat
     8041 agcttggtct gtatcatcac ctttttcgtt aagggttttt gctttgtaaa aagcaaagcc
     8101 ataaagagca gcttgtataa ggataaggat agttccaacc attgacaagg ttgttgtgaa
     8161 ctggttaacc aagccgataa cagaaaggat aagtcctaat atgtaaggaa tataactaac
     8221 cgttaatcct tgttttagtt tggataagtt ctgagtacag aagatgataa ttgcaataat
     8281 agcaaggaca tttaagacag ataagaaaat cataaatggt gtcatgctgc tttgtagctg
     8341 tgccaattgc tcagttgtta attgggcccg aaatgcttca ttcttgagtt ggctttgtaa
     8401 gtagaaaaaa cctgcaaatc ccattaatga cagtacaact ccaagcccgt ttagaataat
     8461 aatggcaata gtagacgttt tgagtgcctg acgcactttt tcataagaaa tcatataatc
     8521 ctcctagagt ccttaatccg aaattgtaca atgtgtttaa tacaaaaata ttataccata
     8581 gattaatcaa aaatgtccgt ttttctagga ttttttctgg aaaaagaggg caatatctga
     8641 ctctgtaagc cctatcatcg aatcgatagt cgaataattc tcaggtgtta acctaaaatc
     8701 atcattatct gactgagagt tctcagtggg gttagagttt gttgattgag aggaagattt
     8761 ttgagagtcg tcctcaaggg ttgccgtatt agaaaaaagg gaggtctgct cttttgattt
     8821 tagctcttgg ttaggttgag aggaggctaa gtcggattgt tcttggaagc gttcgatcaa
     8881 gtaggtttgg cgtttatcgc cttcgtgttt aatagcatac tcaaaggcag tatattctcc
     8941 caacagaatt aacttacttt tagaccgagt aatggccgtg taaatcacat ttcgttgcaa
     9001 gagtcggccg ctttggcgtg tgataggtaa aatcaccact tgaaactcac tcccttgcga
     9061 tttatgaatg ctcatggcat aggccagggt taattttagc cattcatttc tagggtacgt
     9121 gacttcgcta ccgtcaaaat ctaagattaa ttcgtcttgt ttagattcgg tgtatttagc
     9181 aggaatcaaa tctgtaatat aaccaatatc tccgttaaag acattcaact gagcatcgtt
     9241 gactaagtgc aggactttat cacctttacg aaaatgcgta tcattgaaca gaaattctgt
     9301 ttgtccttgc aagggattga gaagttcttg catgagttga ttaagatgat tgatgccagc
     9361 ttgtcctttg tacataggcg ctaaaatttg aatttcctca gcagggatgc cacttttaat
     9421 agcagatagg acaatttttt gaatcatgtc agggataaag gctgcttggg cttcaaaata
     9481 agaacggtca gcttttttat cacgaaagtc agcagctaag attcctcgac gcatctgatc
     9541 tgccaaattg acaatagtag attcttggga ttgacgaaat atcttttgca aggcgatttg
     9601 gggcaggcta ttgactttta aaagatctga caggacttga ccgggtccaa ccgaaggaag
     9661 ctggtcacta tctccgacaa taatcacttg agttgttgag ttaatcgctc ctaacaactg
     9721 gttggcaagc caggtatcca ccattgaaaa ttcatcgaca atcagcaggt cgcagtctag
     9781 ataatcctcc atggcttggt aatcgttgtc gccattgagg cctaagtgcc tgtggatggt
     9841 tgcgctagga agtccagtca actcattcat gcgacgtgca gctcgacctg ttggagctgc
     9901 taacaagata ggaaggtctt ttttatccaa atcaatctga tgcaggttcg cgtaagcctg
     9961 caaaatgcct cgaataacag ttgtttttcc tgttccggga ccacctgtta ggagaaagac
    10021 cttgctggtt aaggccttag taatggcttc ttgctgcact tggtcatagg tgatggcaaa
    10081 gtctttttga acggcttgga tggttgtttg aatggtatca tgactaaatg actgacttaa
    10141 aggggtatct aagagacgag agatgttgct agcaatccct tcttcagcaa aataaaggct
    10201 agcatcaaac agtttggtgt cactgttttt gattttgccc tctatgatca actcgcttag
    10261 ttgttcagcc acagcagcag gatcacattc tacttgacgt gcatcttcaa ggagtgtgat
    10321 ggcaaagtct aataattgtc gtgcttggac ataagtgtct cctcgactga cggattcttc
    10381 caagaggcag tgcaggaggg cagcacgaaa acgttttgga gagtcgctct caatccctaa
    10441 gttttcagca agagcatctg ccatcttaaa cccaaaacct tgtagatctt cgactaattg
    10501 atagggattt tctttaacaa ggtcaagagc ctcttctttg tacttctcaa acgcttgaaa
    10561 ggcaaaacgg ttgctaaggc caagttcgac gagaccagca atcagttgct ctgtgccata
    10621 attcaatttc agtttagcga caaaggcttg acgattagcc ttggacagac cagagatagt
    10681 ttctaatttg cttgggtctt ctaaaatatg gtcaatggta ttatgaccat atagcgcaat
    10741 gattttctcc gctgtttttt taccaatccc cttaaaatgg tcactagaga aataattaac
    10801 caaaccagat gaactaggtt tgattttttg gtaacggctt agtttgagtt gctgtccata
    10861 tttagggtgc tgggtcaatt ctccccaaaa ggtgtagtca tctccttcaa taatgtcagc
    10921 catcgttcct gtgataatga tttcaaagtc gtcaatgtca ctgtctgtat cctcaatggc
    10981 aaggaggaga attttaaaga aattagcctg gttttcaaaa atgatacgat caacagtacc
    11041 tgtaaaaacg tattccatag taactctttc tatttataca agataactga aaacctgccc
    11101 aaaggcaggt tttttctaat taaaaacggt cattttattg agtggccaaa aacgagcttt
    11161 cacttcaccg ataaggtttt cttttttgaa actaccaact tcgcgactat ccctggaaac
    11221 aatacgatca tcaccaagaa gaaggtattc tccttttgga acactcatct caaagcgtgt
    11281 ttgtccctca gaattagttg taaaagcatc agatgtttct gctaactgtt ggaatagggt
    11341 attataggcg taagtttttt ggagtttatc gtttttaaat tgttttagat actcagccaa
    11401 atacggctca actgtttttt taccattaat ataaagtgtg tcatcattat aagaaatggt
    11461 atcgcctggc aatccaataa ctctttttac gatttctttc ttttgtccat tttcttcttc
    11521 ctgagcaact acaatatcaa agcgatcaat cctagcttga ttaaaaacga taaggcgttc
    11581 gccatgagct agagttgggt ccatagaatg gccgtctact ttaacagcct gccacaaaaa
    11641 aagacgagat agaccaaaga gaattaaaaa gagagtgaat gggccccatt ctttaataaa
    11701 ctgtttcata tgattccttt tatttagtga gaagcgcttg cgctttgtga gtgttggcga
    11761 agtgaagttt agcactatat tctaagctag acataccata agcggctaag agttggctag
    11821 caactttatc tgaagctgaa ccagcaccgc ttgggagttg gtaacctaaa tcttgaccta
    11881 attgatcgag attgtctaaa aatagatttc tagcaatgat agagctaaca gccacagcca
    11941 aatagtgact ttctgccttt tcttgaaagg ttagaggatt tgggaaatgg tttttttcct
    12001 tttttagatg cttttcataa tttgattggc tagtgaaagc atcaataaca atttgtttag
    12061 gttgaatgcc tttttgtaat aacaaaaaaa tagcttggtt atgaagagca actttgatag
    12121 aaatggcatt atatggttta cttttaccaa ccaattcgtt atatttttta ggtgatagga
    12181 gaagtgactg atgaggtatt tgtttctcta acagaggcgc aatctgttga atggttttat
    12241 ccgataattt tttagaatca tcaaccccaa gctttttcag gaaggaatga tctttaggat
    12301 cgacaaaact tgccacaaca gcaatcccac caaaataaga gccattaccc acctcatcac
    12361 tgccgattat aggtatatct tggctattgt tgcttgcttc aaaaactgtc ttagctactg
    12421 gaagattaag ctcttgagct aaagcatttg caccgtttcc ctggagaact aattttccgg
    12481 atttgtacag caaaacagta accccatttt ttttagcagc aaaggctaca taggtatttt
    12541 ggctactgat ggtgtaggag gctagctgtt tttttaaatg tttactcagt atagcatcta
    12601 ttttcaaaac taaggtattc ataataatta ttttaacata aaatagttga ctatggttaa
    12661 ttcataagtt tatttaacaa agaaaacaga gactcgaatg tttagacggg ttagggcaat
    12721 ggtgttgact attttacgaa agtttcattt tacggtataa tgctaatgag gtgaccctat
    12781 gaaaagtatt aatcgctaca aattcacctt tggtgaaaaa acattaacat tgacaactga
    12841 caaagacaat ctctttatgg aagaggttga gcgggtagct aaagaaaagt atcaggctct
    12901 taaaaatcat ttaccagaag ctgatgacga gactgtcgct attttaatgg ctatcaatac
    12961 cttgtcaaca caattaagca gagagattgc cattgaaaaa atggaagctg aaattttaga
    13021 tttacgtcaa aaaaccttgg ttggtcttca agaaaaagct aatcaaatag atgtgaatga
    13081 ggtttaatca tgttatcttt attgatagtt cttgttttaa cttggaattt ttatattggt
    13141 tatagtagag gtattattct tcaatcgttt tatgtcttag gagccctact ttctcttttg
    13201 gtagctaatc gattttatat tggattagcc cataagctaa cgctttggat tccttattct
    13261 aacccggtag aaggaacatc agtctttttc tttaaatcag ttgatatctt tgttttagat
    13321 aaggtttatt atgcaggcct tgcctttttt atcattttct tattggggta tgcattgagt
    13381 cgctttcttg ggatttttgt tcatttcctt ttgctagatt attttgataa ccagtggaca
    13441 aaatgtttga gtggcggact agcgcttcta gtatcgcttc tttttttgaa catgttactg
    13501 tctatttttg caacggtccc tatgcctttt ttacagcact atttgcactc tagttttctt
    13561 gctcgtttgg tgatcgaaca ccttccacca ttgacaataa ttattcaaaa attgtggatt
    13621 caagccatta tttgatatcg ttttgtgact aatataagaa cctggagtga gtttcacctt
    13681 taggttcttt atttacgaga aactgtttag aaataaagag aacgcatggg aacttagtga
    13741 ctttcttgcc ccattttcca tcattttagg tataatgaag agtatgaata acaagatttt
    13801 agagcagtta gaatttaaca aagttaagga attgctatta ccttatctca agacagaaca
    13861 atcccaagaa gaattattag agctggagcc gatgacggag gctcctaaaa tagaaaaaag
    13921 ttttaatgaa atttctgaca tggaacagat ttttgttgaa catcactcat ttggcatagt
    13981 cagcctaagt tcaatctctg agagtttaaa acgcttagag ctttcagctg atcttaatat
    14041 tcaagaactt ttggctatca aaaaagtttt acagagttct tcggatatga ttcactttta
    14101 ttctgatttg gataatgttt ctttccaatc tttggatcgt ttgtttgaaa atttggaaca
    14161 attccctaat ctgcaagggt cttttcaagc tatcaatgat ggtggttttt tagaacattt
    14221 tgcgagtcca gaattagagc gtatccgtcg tcaattaaca aatagtgaac gacgggttcg
    14281 tcagatttta caggatatgc ttaaggaaaa agcagagctt ttatcagaga atctaatcgc
    14341 tagtcgtagt ggacgaagtg tcctaccagt aaaaaatact tatcggaatc gtatttctgg
    14401 tgtggttcat gacatctctt cttcaggaag tactgtttat attgagcctc gtgctgtagt
    14461 tacactaaac gaagagataa cgcagcttag agctgacgaa cgccacgaag aaggtcgtat
    14521 tttacacaca ttttcagact tgttaagacc tcatgtcgcc actattagaa ataatgcatg
    14581 gattcttggg catcttgatt ttgtaagggc taaatatctt tttatgtctg ataataaggc
    14641 gacgatacct aagatctctg atgacagcac gttagcatta atcaatgttc gtcatcctct
    14701 gttaagtaat cctgtggcta atgacttaca ttttgatcac gatttaactg caattgtcat
    14761 cactggtccc aatactggtg gtaagacgat tatgctaaaa acactcggtt tagcacaatt
    14821 aatgggacag tccggtttgc cagtattagc ggataaaggt agtaaaatta cagtatttaa
    14881 caatatcttt gcagatattg gcgatgagca atctattgaa caaagtctat caactttttc
    14941 tagtcatatg acgcacatag tcagtatttt aaacgaggct gaccgcaata gtttagttct
    15001 ctttgatgaa ctgggagcag gaacggatcc tcaagaaggt gctagtttgg ctatggctat
    15061 tttagaacac cttaggttaa gtcatatcaa aacgatggcg accacgcact atccagaatt
    15121 aaaagcttat gggattgaga caaattttgt agagaatgcg agcatggaat ttgatgccga
    15181 aacgcttagc cccacgtatc gctttatgca aggagttcct ggacgatcaa atgcatttga
    15241 aattgcttct cgccttggtt tagctccatt tattgttaaa caagctaagc agatgacaga
    15301 ttctgactca gatgttaacc gtattattga acagttagag gcacagacac ttgagacacg
    15361 tagaagactg gatcatatta aagaagttga acaagaaaac ctcaaattca atcgtgcggt
    15421 taagaaactc tataatgaat tttcacatga gcgcgataaa gagttagaaa aaatctatca
    15481 agaagctcaa gaaattgtag atatggcttt gaatgagagt gatactatct taaaaaaact
    15541 caatgataag agtcaattaa aacctcacga aattatagat gctaaggcac aaataaaaaa
    15601 attagcacct caagttgatt tatcaaaaaa taaagtctta aataaggcta aaaaaattaa
    15661 agcagctcgt gcccctagaa ttggtgatga tattatagtg actagctatg gacagcgagg
    15721 tactttaact agtcaattaa aagatggccg ttgggaagca caagtgggaa ttatcaaaat
    15781 gacattaaca caagatgaat ttacccttgt tagagtccaa gaagaacaga aagtcaaaag
    15841 taaacagatt aatgtggtta aaaaggctga tagttctgga ccaagagctc gacttgatct
    15901 tagaggtaaa agatacgaag aagctatgca agagttagat cattttattg atcaagcatt
    15961 gcttaacaat atgggacaag ttgatatcat tcatggtatt ggtacaggcg ttatccgtga
    16021 gggagtgaca aaatatcttc gtcgtaataa gcacgttaag cattttgctt atgccccaca
    16081 aaatgcaggg ggatctggcg ccacaattgt aacgttaggg taaattatgg aatcatatga
    16141 acaattttat gccaaactat ctcaaccttt tagaaaatcc ccacagttaa taattctttt
    16201 aaatttttta ctaaaaatag tcacaggaat gatgtacata ctctatccat cttttctgat
    16261 atttacgctt tggcaaggga tgacatttca attatggtta aggctattaa ttatccctgc
    16321 tgttggcttt atagctttgt cttacattag gaaacgcttt gattgtccac gaccgtatga
    16381 aaaatggaat attaaacctt taattgataa ggatacaaag ggaaggtcaa tgcctagcag
    16441 acatgttttt tcagctacga tgattagtat gtgtttgtta cgatattatg tttactttgg
    16501 aatagtttgt ttaatattat cggttctatt ggcaatttgc cgtgtgatag caggtattca
    16561 ctatcctaag gatgttattg taggttacct tattggcttg atgttagggc tttgtttatt
    16621 gatttagcga gttcgtagca aatagtttca aaaacaacta aattcttgta caataggctt
    16681 atcattaaga tgaaatggag aaaaaagatg gcattagaag taacagatgc tacatttgta
    16741 gaagaaacta aagaaggttt agtactcatt gatttttggg caacctggtg tggtccatgt
    16801 cgtatgcaag caccaatctt agaacagtta tctcaagaaa tcgatgaaga tgaactcaaa
    16861 attttgaaaa tggatgttga tgaaaatccg gaaactgctc gtcagtttgg tattatgtca
    16921 attccaacat tgatgtttaa aaaagacgga gaagtggtta aacaagttgc aggtgttcat
    16981 actaaagacc aactgaaagc tattattgct gaattgagtt aactaaaata aagcccagcg
    17041 tcacgctgag ctttatttta ttatctacta acttcaatgg aataaccatg taaacataaa
    17101 acgacttgca aaagcaaaag caacagtaat gaggaaaaat acagtgacaa taaggggttt
    17161 ctgataagga agttttgctc tttcttctcg tttttcgatt tcgtctaata attttccatt
    17221 tgaaacagct aggatttcct tataaggttg cacatcatat tttttcttaa aacgttccac
    17281 acgattagca taagtcatag taattatcga taataaaaca aaaatgccaa gtcctagcca
    17341 ctctaagaaa tagactaatg gataacttga aatcattaat agtatcatca atgttaccat
    17401 tactttggta tctcgtttaa aattcttttt atcaacctga gtgatggtat atttcatttt
    17461 ctcaatgtct cctttaatga gttggtctaa gctcacttga aaaatttgac ttaggagtaa
    17521 gaggctatgg atgtctgggt aagttttgtc attttcccag ttagatatgc tctgatagct
    17581 tacatagact ttctcggcta atttttcttg cgagagcgct tgttgtttac ggtagcgaat
    17641 aatttgctga ccaatttcca taaatatcac cggaaaactt tacagcggcg taaaggaatt
    17701 gtaaacaaaa agtgaaccgt tatagttcac ttgcgattaa gcatttttct gttttaagta
    17761 ggtctctagc attttctttt gtggtgttgc aaagggatac agagaaaagt cctcaattgc
    17821 tacccacttt aggtgaggag cattaggtaa atctgttgct ttgaccaccc cctctatgag
    17881 ttctattgtc cacttctggt gactaaaagt gtgcttaata tttggaaagt gattatcagt
    17941 ccattggggc tttaattgat attctctttc gaaagtttca ttttgggttt gccagattat
    18001 ggggttggat tgattgtcat caaataaatc taattgttga gaaagaggtg acgtttcgat
    18061 aataggaaat gaccaaaaac ctcctagaag tctcccttta gtatttttct ccagtagata
    18121 ttggccattt tggttacgaa tgacaaaagc ctgaattcgc attggtttag gctttttctt
    18181 tggatttttt atgggatatt taccataagt accattcaaa taagcagcgt taaagaaacg
    18241 aatagggctt tcatcaggcc taggtgtctt agcggattcg atgtctgtac ctagatccat
    18301 aagtgcctgg ttaaaatcac cgggtctgtc agggtcaatt agtatttcca taatagcttg
    18361 aaaaatttta cgatttttag ggtcaccgat atcataatta acttcaaaca aacgagccat
    18421 gacacgcatc acattgccat caacagcagg ctctggtaag ttgaatgaga tgctggcgat
    18481 agcacctgca gtataaggac caatcccttt taatgaagca atatcatcat aagtgtgagg
    18541 aaatatgcca ccaaaatcaa ccatcacttg ctgtgcagcc ttttgcatat tacgcacccg
    18601 agaataatag cctagtccct cccatgcctt tagtaattgt tcttcaggag cgtctgccaa
    18661 atcttttatt tgaggaaacc actctagaaa acgtttataa taagggatta ccgtattaac
    18721 ttgtgtttgt tgcaacatga tttcagaaac ccaaatatag tatggattag tagttcttcg
    18781 ccaagggaga tcacgttttt cttggtcata ccaccctaaa agtgtgcgac gaaaagaagc
    18841 gattgtctca ttatcccaca tattaatacc ataatctttt aattcaatca tgttgttatt
    18901 gtaacacagg agtcttaaaa agagtaaaaa aagattttag gtatttgcga aaaagaaaag
    18961 gaaatattat aataatatta ggtttacctc atctaaatgt tgatttagaa taactgaaat
    19021 taacgataga agcagacagg ttttaatttg gaggggacat gttccagaaa ttaaagaatc
    19081 ttattgatcg gtattcgtgg ttcgttttta taatgatgat tcttgtgtta tttcttaact
    19141 atatttatcc atttgatcct tctttgagcg gactacagtt ggaatggctg atcatgacgc
    19201 aagtttttcc tggattaggt atcgtatgtg ccatggttgc tattattttt aaaaagttta
    19261 acctttttgt tttggcccta attttatatt ttgcactttg gttaaaactt ttccttgctt
    19321 tttctttttt cacctatttt ttaggagtct gactaagtgc caatttttaa aactcgtgaa
    19381 agaaaatgtt tgacaaaact tagtcaagta gaaaaagttc cttgctttta attgactatt
    19441 agtgtacaat aagattctgt gagtatacct cacttactcg ctccaaaagt agtgagtcat
    19501 tagaccaaaa ggaggaaaat atcaatggct aaatacgaaa ttctttatat cattcgtcca
    19561 aacattgaag aagaagctaa aaacgctttg gtagcacgct ttgactctat cttgactgac
    19621 aacggtgcaa ctgttgttga atcaaaagat tgggaaaaac gtcgtcttgc atacgaaatc
    19681 aacgatttcc gtgaaggact ttaccacatc gttaaccttg aagcgactga cgcagcagct
    19741 cttaacgagt ttgaccgtct ttcaaaaatc aatggtgaca ttcttcgtca catgatcgtt
    19801 aaacttgacg cttaattcca actaagaggg tagtttatga ttaataatgt agtactagtt
    19861 ggtcgtatga ccaaggatgc agaacttcgt tacacaccaa gtcaagtagc tgtggctacc
    19921 ttcacacttg ctgttaaccg tacctttaaa agccaaaatg gtgaacgcga ggcagatttc
    19981 attaactgtg tgatctggcg tcaaccggct gaaaatttag cgaactgggc taaaaaaggt
    20041 gccttgatcg gagttacggg tcgtattcag acacgtaact acgaaaacca acaaggacaa
    20101 cgtgtctatg taacagaagt tgttgcagat aatttccaaa tgttggaaag tcgtgctaca
    20161 cgtgaaggtg gctcaactgg ctcatttaat ggtggtttta acaataacac ttcatcatca
    20221 aacagttact cagcgcctgc acaacaaacg cctaactttg gaagagatga tagcccattt
    20281 gggaactcaa acccgatgga tatctcagat gacgatcttc cattctagtc agaatggatt
    20341 gaacgaatac gaaattattt gagttcgggc taaaaattta gaacttagaa tttagatcaa
    20401 ctccctagga aatcaagatt tcttgccagt tttctaaaat tcagtcgttt tttaagagcc
    20461 cttaacatca taatttataa aggagaaaat acatggctca acaacgtcgt ggcggattca
    20521 aacgccgtaa aaaagttgac ttcatcgcag ctaacaaaat tgaatatgtt gattacaaag
    20581 atactgagct tcttagccgt ttcgtttcag aacgtggaaa aattcttcct cgtcgtgtaa
    20641 caggaacttc agctaaaaac caacgtaaag taacaacagc aatcaaacgt gctcgcgtta
    20701 tggctcttat gccttacgta aacgaagact aataaacaaa cagtccattt cggactgttt
    20761 tttagttgac taggactagt ctaggcgtat aggtgagttt tttctaaaag agagaatgat
    20821 agtaaacgaa aatccttgcg tagacagcaa ggatgaaatg attgttattt gttaacaggt
    20881 gacatagtgt tttgaatgtt gtatttgcgt tgtaggtaat agcgtaaagc aagggaaaca
    20941 ccaccaataa tgagcaaggc aagaggaggc aactgtgggt taagagaggt tggtaaaaag
    21001 gcagttgcag agtacaaggc aatccaaatc aacatggcta atgataaggc tgcgatgatt
    21061 ttgaaccaac ttggacgaag gctcttatct tttccgagat gacggtaaat aaagtaataa
    21121 gtagcgtaca tggatgctcc tccaccaaaa ccaagtgcca gtaaggaaat caatcctgta
    21181 actgtagcgt ttgtgttgaa gaaggtcatg atgccattta aaagggcaac aattccgatg
    21241 aatagtaaag aggtatctag ccacatcaac caaggattgg tgtttttgtc agtttctgct
    21301 gctctttgac tagggtcttg gctaaaagaa gctgcccaag cagtcggagt gcctaataga
    21361 tttcgagcgg tgacaccttt tttctggttt tctagaatag ctggaatggc ttcttcgaga
    21421 atcagcttaa tatcctcatc agacttgccg tcttggatta atttgttcgt agcggtgtgg
    21481 ataaattctt gatttttttt ggtaagttct tgtaattcca tgggaatccc ttctttatat
    21541 gggttagtta aagtaagtca atggtattag ctagttgtaa tgaaactttg gagctagccg
    21601 tttgacacag agttttgtca ggtcttaaac gtattctgca gcttgctcca ctctagaacc
    21661 atttttttct aataaagtaa atcacaacaa aggaactaag gagcatagca atcaaggtaa
    21721 tataccaaaa agcatgttct aggccattta gtggtaacca gttgttttgg aaattcatac
    21781 cataggcgga gaaaatcaca gttggtatat ctaaagccat ggtcatcaag gccagtgttt
    21841 tcataatagt gttctggttg ttgttgataa tagaagcagt ggtttcagtc atggcattca
    21901 agacattttc ataaatgcca gccatctcaa tggcctgttg ggtttcaatc aaggtatctt
    21961 ctagcaaatc ctcgtcctca atgtatttct tgagagagct tgttgagctg gataatttct
    22021 tgacgatgcg ttcgttgaat ttcaaggaag cctttaagta gacaatggat ttttcaagtt
    22081 ccatcatgtc gataagttct tcatttcgag tagctgcttc aagctgagct tccaaacgtt
    22141 cgctttggcg atcaatggtc ctcaaggcag tcagaaagag ctcggcgttg cgatatagaa
    22201 tctgaaaaac aaaacgtgtc ttcatgaagg tatagaaatt tttgacccgg cggttgtgaa
    22261 aatgatcgaa aagggtcatg tcatgcagac aagttgtgat aacagcattt tccgttacaa
    22321 taatgcctaa aggcatagta atatagtaac ttttattgtt acgttcttcg tatatcggga
    22381 cgtctacgat aattagggta taatcatctt ctacggcaat acgtgaggtt tcttccacgt
    22441 ccaaaggggc gcgcaagtca gaaatatcaa tgttaaattg atcggcgatc ttcatagact
    22501 cttcttggga agggttaacc agtttaatcc aagctcctgg ctcgaatgtt tctatttctt
    22561 tgaattcaat tgccgatgaa agaaacattt gtttcatgac aagccctcct ttttgatgat
    22621 tgactggtag agattgacat caaaccttga taataaagcc ttctcaagga ttttctgaaa
    22681 aattctcaat acttatctat tataccaaaa ttcgcaaagt ttggggcaat aggttatcag
    22741 aaatttttgt tataatataa ggttacctct cagaaccatt gataccaagg ctttttaaaa
    22801 gtctaaaacc cgacttacta cgttttccgc tacgtttgtg ttaaacagcg acaagttcag
    22861 ccaatttgtc agcaacatca tcctttgatt ttttggacaa gtgagtatat aaatccattg
    22921 tgattgctat tttagcgtga cctaacctct cttgtgcaat cttagcggga atcccagcct
    22981 caaacagtaa cgaagcgtgg gtgtgtctaa acccgtgggg agtaatcctt tttaatccat
    23041 gcttcaatat aagtttatcc agtctgtttc tcacagtgtc gcaagaaaaa ttaaacaatt
    23101 taaacgatcc gtgtaaaggt ttgatacttt gcttaatata taatttggct aacgacatgg
    23161 tctcagcgtc cattgatata gcgcgagtgc tttttttggt tttaggagat tgtacagccc
    23221 atccttcttt tgtataggca atagtttttg taatgctgat agtattgtta tggaattcaa
    23281 agtcggtatc ttttaaagcc attaattcac caattctaaa accgccataa ctcagcaatc
    23341 tataaatcag acgcatctct agactttctt cagcttccac aatcctcaag aaagtattca
    23401 attcctcttt tgtgtaatat tttttggtga gtgtattcac gtttttgtaa gtgcactttg
    23461 atctcaatgt cttgctcata gggttattat caataatacc taaattgaca gcaaatttaa
    23521 aaattctgtt tgctagactt agataaagcg tataaccgct atatcgagac aagcaattca
    23581 caaatttttg acaaatcatg acagttatct tcgtcatttg cagttgtcct aaatgtggct
    23641 ttaaaattgt tttatagtag ttttggttaa ccagaaatgt tgacggtttg actgtattct
    23701 tatactgttc aaaccacagt ttagctacgt catcaaatgt agttctatca ttgcttttcc
    23761 aggcgccttg acgctgaaaa tcatcaataa gttttacctc ggctctttta gcttctcttt
    23821 ccgttttaaa accttgccta gttgtcctga cttgtttacc agtcacggga tcaaccccga
    23881 ggtatgctct taagcaatag gcagttgtgc catctttttt agtgtatttt ttaatcattg
    23941 tttttcctct ctctttgcgc tggggagtgc tgattttgag ataggatatt ggcatcacct
    24001 cctaaaaatg gtataattag gtacaagaaa acgagccatt taatgcttgt ttcttattga
    24061 ttgcatatcg cctcacgctc tcctcgccaa atttgagcgt ggggcttttt tatttgtctt
    24121 tattattgtc ttctaggtgt ttaatagcat aatttacttc ttttttagta aaaccaccac
    24181 cgtcgctgtt agcagaaagt tgttctttta tcgtttcttt tgatgcgccg aatttttgca
    24241 tacttttagc ttgggcaagt gcattctttt tccagtttat ctttaatttt ttcgctgcat
    24301 aatcaacagc tttcttttca aatttacttt cgttaatcat catgtcataa aatgcttttt
    24361 ttgacatatt ggcgtctttc accatttttg ctgtttctaa agcgttttgg tattcctttg
    24421 atacagcaac tacagtaaca gattgactaa ataacttgtt agataattct ggagttgtag
    24481 ctgtagccat aactccaaca acaactatcg caataattga acattttttc ataataaatc
    24541 tccttataaa aataatctta atttttaaaa tttacccaaa aacttcttcg ttggacggga
    24601 ttgtcatttt cccgaataac tccccacaac ctcaccaata attctaaagt cgctatctgc
    24661 gtcaatcgga atgtcatcgt acttgctatt taaactgtgc agaaatgccc cctcggcgtt
    24721 tataagcaac tgtttgatat aagcgtcacc atagtattca aagacgccta tatcgccatc
    24781 cgtgaggtct acggatagct taacaaatac ataatcccca gagtgatact tcggttccat
    24841 ggaatcacca taaacaggga taacaaagtc tgcgtcataa tcgactggta actcaatttt
    24901 ttctacttgt acatcattta gatactgacc tgtaccagct gaagctgcgt ggtcgtagta
    24961 gttgtaagaa aataattcta ctactgtatt cttactatct tctactgtgt tttgttcaga
    25021 taaaagattt tcaccgtaac ttatccaatc actgtgacga ggttctttga gttctttgtc
    25081 taattggatg acgatgttag aagtagcaga aataatttca ggagaataca tttctactaa
    25141 atcagatttt tcaacgttaa aatagttagc catcaattcg attttgtcga ttcttggata
    25201 agttttggcg tttacccaat ctaaaactgt gctgtactta aaattcatcg cttttgaaaa
    25261 ctctttaggg ttcatattct ttttttctaa atgccttctt atatttgaag ccattatctt
    25321 tttatttccg agagacccac tcataataac acctcatttc tagttgtatt ataacggata
    25381 aaccgtgctt ttgtcaaaaa ataatttaaa aaataaaaaa aatagcagtt ttgtgttgac
    25441 aacacggttt aaccgtgtta aactataatc aagcttaagg aattaagcaa aacgaaagga
    25501 ggtacagctg atgaagtcta ggctaaacaa aaagcctaaa cacaaagaac tagaagtcga
    25561 aatcaagatt ctttggttta agcttagaat taaatatctg attactaggt aatcggatag
    25621 ggggtgaaat acccccaccc catttagggg taagtttagt ttagcacatt ggctgtatct
    25681 ccgcaagaat gaaaggagag gttatgagag aacgaaatga aaagttcatc ggagtgctaa
    25741 aagatatgca catgcaaatt ttgcgagact tcacaaacga agaagacccc ttaacaaggt
    25801 gcgagcttgc taaagggttc ctcgaaattg gtgactattt atccaaacaa tgactcgcca
    25861 cgctcaagag cttcggctat aactttacca ttattgattt gttcttgctt ttcggcttca
    25921 acgaaaggta aattatgttg cttaatagct tccacagagt cttcgtaaag tttgatttta
    25981 tcttcgatag acaaagtagg gctagaagaa gcgacaatag cgagagctaa atcttttgaa
    26041 ttagaaattt tcattagctt atcctccttt ctgctaggat aagttgatta taacattttt
    26101 aggaggtaca aaatgagttg gaaaaaacta atgcttggcg acttagaaca cacgtttact
    26161 agtcgtgatg gcaaggaaaa aacaagcgtt gaatttgaag gcggtgtatt accagctctg
    26221 ttagtgctag gtggtatcac ttggctgatt gcttggttta ttacaaaata aaaactcccc
    26281 aaaaggagtg gaaaggaggg aaggcatgga aaaaattaaa tacggtgtac taggttcttc
    26341 caagatattt cacacaagaa aatctgctct cactcatgca aatcttttag gatatcctcg
    26401 tagtgcggtt tttttgataa ttccacaggg aatgacgcag aaaatgattg ataaggtgaa
    26461 ttaacatatc ctgctttatt agctttaaat agttttttct ttttagctac tttaaaagca
    26521 acaaagagtc tatctgtgat ttcagacgtt tgtatgatta aatccatcct tgtcatactg
    26581 tgggctttta atactccaca atctgcctct ggaacctcga taaggattgt attgccatca
    26641 ggctgtactg cggctatagc ctctctacct gttaaatcgt taattatatt attttgcttt
    26701 tggtaataat gctgatattt tctgttttta tcaaaaacaa tcaagtcgaa gtagcttaca
    26761 tcaacattag aaggattgat gattttaatg ttagctctta atgtaccatt tggattatat
    26821 atgctttcac cgttgtctaa aataacgctc aaaatccaat ctgaaacagg agcagcaatt
    26881 aactcgactt gtaagttatt tcttcggtag tttgaataag atagaaacag agctattaaa
    26941 gctatccaat tttttattag atattcactt gtaaacttaa aaacacacaa taaaaaatta
    27001 aaaaaattca tttcaacctc gcaattttta tttaaattat accacagaaa attttaaagc
    27061 tatgt
//
LOCUS       DAUDYX010000023        26413 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.023, whole genome shotgun sequence.
ACCESSION   DAUDYX010000023 DAUDYX010000000
VERSION     DAUDYX010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26413
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.023"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            75..710
                     /locus_tag="VHD02_000967"
     CDS             75..710
                     /locus_tag="VHD02_000967"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEQ5497585.1"
                     /translation="MITGKLITVEGPDGAGKTTVLEQLIPLLKQKVAQEILTTREPGG
                     VAISEYIRELILDINHTTMDPKTELLLYIAARRQHLVEKVLPALEAGQLVFIDRFIDS
                     SVAYQGAGRGLIKADIQWLNEFATDGLEPDLTLYFDVPSEIGLARINANQQREVNRLD
                     LETIEIHQRVRKGYLALAKEHPKRIVTIDATKPLKEVVSVALEHVLALLLA"
     gene            728..1603
                     /locus_tag="VHD02_000968"
     CDS             728..1603
                     /locus_tag="VHD02_000968"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEQ5497586.1"
                     /translation="MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEEMALFLAK
                     VIFCEQKKDQKPCGHCRSCQLIEQGDFADVTVLEPTGQVIKTDVVKEMMANFSQTGYE
                     NKRQVFIIKDCDKMHINAANSLLKYIEEPQGEAYIFLLTNDDNKVLPTIKSRTQVFQF
                     PKNEAYLYQLAQEKGLLNHQAKLVAKLATNTSHLERLLQTSKLLELITQAERFVSIWL
                     KDQLQAYLALNRLVQLATEKEEQDLVLTLLTLLLARERAQTPLTQLEAVYQARLMWQS
                     NVNFQNTLEYMVMSE"
     gene            2267..2590
                     /gene="yabA"
                     /locus_tag="VHD02_000969"
     CDS             2267..2590
                     /gene="yabA"
                     /locus_tag="VHD02_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEQ5497587.1"
                     /translation="MNKKELFDAFDGFSQNLMVTLAEIEAMKKQVQSLVEENTILRLE
                     NTKLRERLSHLEHETVAKNPSKQRKDHLEGIYDEGFHICNFFYGQRRENDEECMFCRE
                     LLDRK"
     gene            2595..3458
                     /gene="rsmI"
                     /locus_tag="VHD02_000970"
     CDS             2595..3458
                     /gene="rsmI"
                     /locus_tag="VHD02_000970"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEQ5497588.1"
                     /translation="MQVQKSFKDKKTSGTLYLVPTPIGNLQDMTFRAVATLKEVDFIC
                     AEDTRNTGLLLKHFDIATKQISFHEHNAYEKIPDLIDLLISGRSLAQVSDAGMPSISD
                     PGHDLVKAAIDSDIAVVALPGASAGITALIASGLAPQPHVFYGFLPRKAGQQKAFFED
                     KHHYPETQMFYESPYRIKDTLTNMLACYGDRQVVLVRELTKLFEEYQRGSILEILSYL
                     EETPLKGECLLIVAGAQVDSEVELTADVDLVSLVQKEIQAGAKPNQAIKTIAKAYQVN
                     RQELYQQFHDL"
     gene            3485..3877
                     /locus_tag="VHD02_000971"
     CDS             3485..3877
                     /locus_tag="VHD02_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497589.1"
                     /translation="MTEDQATILILGVYGIILAVFLLSALLCLIANWRLFKRAGYKGW
                     YSLIPFYAGFVKHKITFGEDNKWFYFIGWVIAIYYYYVHFCYVRAFGGSKGLAVFGLF
                     FPGIASLIVAFGKTYTTQECHPVPHLLN"
     gene            complement(3924..4553)
                     /locus_tag="VHD02_000972"
     CDS             complement(3924..4553)
                     /locus_tag="VHD02_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEQ5497590.1"
                     /translation="MIKEFCAENLTLLPTLDAGQVSRVELCDNLAVGGTTPSYGVIKE
                     ACQLLHDKKISVATMIRPRGGDFVYNDLELKAMEEDILKAVEAGSDALVLGLLTTENH
                     LDTDAIEQLLPATQGLPLVFHMAFDHIPTDHQHQALDQLIDYGFVRVLTHGSPEATPI
                     TDNVEQLKSLVTYANKRIEIMIGGGVTAENCQNLSQLTGTAIVHGTKII"
     gene            4852..5208
                     /locus_tag="VHD02_000973"
     CDS             4852..5208
                     /locus_tag="VHD02_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEQ5497591.1"
                     /translation="MITIYQYPKCSTCQRAMAELKQLVSDFEVIDIKANPPKAQDLKH
                     WMETSGYTIKNFFNTSGNSYRELGLKDKIDQLSLDKAAELLATDGMLIKRPILIKDGR
                     VLQVGYRKPYQELDLG"
     gene            complement(5282..6109)
                     /gene="xth"
                     /locus_tag="VHD02_000974"
     CDS             complement(5282..6109)
                     /gene="xth"
                     /locus_tag="VHD02_000974"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEQ5497592.1"
                     /translation="MKLISWNIDSLNAALTGESPRALLSRAVLDTLVAQDADIIAIQE
                     TKLSAKGPTKKHIETLLSYFPNYLHVWRSSVEPARKGYAGTMFLYKNTLNPVITFPEI
                     GAPTTMDAEGRIITLEFEDFFVTQVYTPNAGDGLRRLDDRQIWDHKYADYLTELDAQK
                     PVLATGDYNVAHKEIDLANPSSNRRSPGFTDEERQGFTNLLARGFTDTFRHLHGDIPH
                     VYTWWAQRSKTSKINNTGWRIDYWLASNRLVDKVKRSEMISSGERQDHTPILLDIDL"
     gene            6344..7525
                     /gene="lctO"
                     /locus_tag="VHD02_000975"
     CDS             6344..7525
                     /gene="lctO"
                     /locus_tag="VHD02_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEQ5497593.1"
                     /translation="MAQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIP
                     KGAFGYIASGAGDTFTLHENIRSFNHKLIVPHSLKGVENPSTEITFDGDHLTSPLILA
                     PVAAHKLANEQGEVASAKGLKEFGSIYTTSSYSTTDLPEISAALGGTPHWFQFYYSKD
                     DGINRNIMDRVKAQGCKAIVLTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGK
                     TMDYVYKSAKQALTSKDIEYIATYSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGG
                     RQLDGGPAAFDSLQEVAEAVDQKVPIVFDSGIRRGQHIFKALASGADLVALGRPAIYG
                     LAMGGSTGTRQVFEKLNDELKMVMQLAGTQTIQDVKAFNLRHNPYDSSIPFDQNALRL
                     D"
     gene            7790..12733
                     /gene="scpC"
                     /locus_tag="VHD02_000976"
     CDS             7790..12733
                     /gene="scpC"
                     /locus_tag="VHD02_000976"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CXC chemokine-degrading serine protease SpyCEP"
                     /protein_id="HEQ5497594.1"
                     /translation="MEKKQRFSLRKYKSGTFSVLIGSVFLMMTTTVAADELTTTSEPT
                     ITNHTQQQAQHLTNTELSSAESKPQDTSQITLKTNREKEQPQGLVSEPTTTELADTDA
                     APMANTGPDATQKSASLPPVNTDVHDWVKTKGAWDKGYKGQGKVVAVIDTGIDPAHQS
                     MRISDVSTAKVKSKEDMLARQKAAGINYGSWINDKVVFAHNYVENSDNIKENQFEDFD
                     EDWENFEFDAEAEPKAIKKHKIYRPQSTQAPKETVIKTEETDGSHDIDWTQTDDDTKY
                     ESHGMHVTGIVAGNSKEAAATGERFLGIAPEAQVMFMRVFANDVMGSAESLFIKAIED
                     AVALGADVINLSLGTANGAQLSGSKPLMEAIEKAKKAGVSVVVAAGNERVYGSDHDDP
                     LATNPDYGLVGSPSTGRTPTSVAAINSKWVIQRLMTVKELENRADLNHGKAIYSESVD
                     FKNIKDSLGYDKSHQFAYVKESTDAGYNAQNVKGKIALIERDPNKTYDEMIALAKKHG
                     ALGVLIFNNKPGQSNRSMRLTANGMGIPSAFISHEFGKAMSQLNGNGTGSLEFDSVVS
                     KAPSQKGNEMNHFSNWGLTSDGYLKPDITAPGGDIYSTYNDNHYGSQTGTSMASPQIA
                     GASLLVKQYLEKTQPNLPKEKIADIVKNLLMSNAQIHVNPETKTTTSPRQQGAGLLNI
                     DGAVTSGLYVTGKDNYGSISLGNITDTMTFDVTVHNLSNKAKTLRYDTELLTDHVDPQ
                     KGRFTLTSRSLKTYQGGEVTVPANGKVTVRVTMDVSQFTKELTKQMPNGYYLEGFVRF
                     RDSQDDQLNRVNIPFVGFKGQFENLAVAEESIYRLKSQGKTGFYFDESGPKDDIYVGK
                     HFTGLVTLGSETNVSTKTISDNGLHTLGTFKNVDGKFILEKNAQGNPVLAISPNGDNN
                     QDFAAFKGVFLRKYQGLKASVYHASDKEHKNPLWVSPESFKGDKNFNSDIRFAKSTTL
                     LGTAFSGKSLTGAELPDGYYHYVVSYYPDVVGAKRQEMTFDMILDRQKPVLSQATFDP
                     ETNRFKPEPLKDRGLAGVRKDSVFYLERKDNKPYTVTINDSYKYVSVEDNKTFVERQA
                     DGSFILPLDKAKLGDFYYMVEDFAGNVAIAKLGDHLPQTLGKTPIKLKLTDGNYQTKE
                     TLKDNLEMTQSDTGLVTNQAQLAVVHRNQPQSQLTKMNQDFFISPNEDGNKDFVAFKG
                     LKNNVYNDLTVNVYAKDDHQKQTPIWSSQAGASASAIESTAWYGITARGSKVMPGDYQ
                     YVVTYRDEHGKEHQKQYTISVNDKKPMITQGRFDTINGVDHFTPDKTKALGSSGIVRE
                     EVFYLAKKNGRKFDVTEGKDGITVSDNKVYIPKNPDGSYTISKRDGVTLSDYYYLVED
                     RAGNVSFATLRDLKAVGKDKAVVNFGLDLPVPEDKQIVNFTYLVRDADGKPIENLEYY
                     NNSGNSLILPYGKYTVELLTYDTNAAKLESDKIVSFTLSADNNFQQVTFKMTMLATSQ
                     ITAHFDHLLPEGSRVSLKTAQGQLIPLEQSLYVPKAYGKTVQEGTYEVVVSLPKGYRI
                     EGNTKVNTLPNEVHELSLRLVKVGDASDSTGDHKVMSKNNSQALTASATPTKTTTSAT
                     AKALPSAGEKMGLKLRIVGLVLLGLTCVFSRKKSTKD"
     gene            13505..14212
                     /locus_tag="VHD02_000977"
     CDS             13505..14212
                     /locus_tag="VHD02_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEQ5497595.1"
                     /translation="MSKKKRKHYQVYEGLRCAMTLCFISGYVNAFTYMTQGKRFAGVQ
                     TGNLLSFAIRLSEQQLKEALQFLLPMIVFMLGQSFTYFMHRWAIKKGLHWYLLSSFIL
                     TGIAFGTALFTPFLPSNVTVAALAFFASIQVDTFKTLRGASYANVMMTGNIKNAAYLL
                     TKGLYEKNHELTHIGRNTLIVILAFAVGVVCSTLLCIAYGEYALMPILMPLLYVNYLL
                     AQEFYHIQTKIKPIKRT"
     gene            14452..16452
                     /gene="metG"
                     /locus_tag="VHD02_000978"
     CDS             14452..16452
                     /gene="metG"
                     /locus_tag="VHD02_000978"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEQ5497596.1"
                     /translation="MMKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEV
                     FYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVKALWQLLDISYDTFIRTTDDYHE
                     EVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGH
                     EVEWVSEESYFLRLSKYADRLVAFFKERPDFIQPDDRMNEMVKNFIEPGLEDLAVSRT
                     TFTWGVPVPSDPKHVVYVWIDALLNYATALGYGQANHANFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERFGLDSLRY
                     YLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGT
                     AFDADLSQLIDAQLADYHKHMEAVDYPRALEAVWTIIARTNKYIDETAPWVLAKEDGD
                     KAQLASVMAHLAASLRVVAHVIQPFMMETSAAIMAQLGLAPVSDLSTLALADFPANTK
                     VVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVITFETFDA
                     VEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKFYPNEQELVGKKLQIVA
                     NLKPRKMMKKYISQGMILSAEHGDQLTVLTVDSSVPNGSIIG"
     gene            16947..17960
                     /gene="nrdF"
                     /locus_tag="VHD02_000979"
     CDS             16947..17960
                     /gene="nrdF"
                     /locus_tag="VHD02_000979"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEQ5497597.1"
                     /translation="MTQHYYERSQSPIEYALSETQKQLRSINWNYLNDDKDLEVWNRV
                     TQNFWLPEKVPVSNDLNSWRSLGEDWQQLITRTYTGLTLLDTVQATVGDVAQIQHSQT
                     DHEQVIYTNFAFMVGIHARSYGTIFSTLCSSEQIEEAHEWVVSTQSLQDRARVLIPYY
                     TGDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKMPNTSDIIRLILRDKVIHNYYSG
                     YKYQQKVARLSPEKQAEMKAFVFDLLYELIDLEKAYLRELYAGFDLAEDAIRFSLYNA
                     GKFLQNLGYESPFTDEETRVSPEVFAQLSARADENHDFFSGNGSSYVMGITEETTDDD
                     WEF"
     gene            17964..18452
                     /gene="nrdI"
                     /locus_tag="VHD02_000980"
     CDS             17964..18452
                     /gene="nrdI"
                     /locus_tag="VHD02_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEQ5497598.1"
                     /translation="MAELIIVYFSSKSNNTHRFVQKLGLPAQRIPVDNRPLEVSTHYL
                     LIVPTYAAGGSDAKGAVPKQVIRFLNNPNNRKHCKGVISSGNTNFGDTFALAGPIISQ
                     KLQVPLLHQFELLGTATDVKKVQAIFARLKHHTHDKQKQTNNLITERTHPCHKPMRHT
                     SH"
     gene            18419..20599
                     /gene="nrdE"
                     /locus_tag="VHD02_000981"
     CDS             18419..20599
                     /gene="nrdE"
                     /locus_tag="VHD02_000981"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEQ5497599.1"
                     /translation="MSQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPN
                     QMAFNSLEDKLAYLINEGYYEQAIFDAYPNDLIKEAFHYAYQQGYRFLNLMGAMKFYQ
                     SYALKTLDGKQYLETFEDRAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAG
                     KKRRGEYISCYLLRVEDNMESISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIEN
                     QATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIK
                     SLALGLVIPDITFQLAKENKDMALFSPYDIKRVYGKDMSDISITEEYDKLLANPAIKK
                     TYISARKLFQLIAELHFESGYPYLLFDDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPS
                     TFKEDLSFETIGEDICCNLGSINIAQAMADAPHFEQLITTSIRALDRVSRVSDLNCAP
                     SVETGNAANHAVGLGAMNLHGFLATNHIYYDTKEAVDFTDLFFHAMAYHAFKASCQLA
                     KEKGAFAGFSLSTYADGTYFAKYLQEDAKPQTAKVATLLQDYGFTLPTVADWQALVAD
                     IKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGSLGRIYVPAYQIDQA
                     NYAYYERGAYEVGPKAIIDVVAAAQKHVDQAISLTLFMTDQATTRDLNRSYIQAFKQN
                     CASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTECHSCMI"
     gene            complement(20804..21556)
                     /locus_tag="VHD02_000982"
     CDS             complement(20804..21556)
                     /locus_tag="VHD02_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyltransferase"
                     /protein_id="HEQ5497600.1"
                     /translation="MLKKRYQLAMILLLSCFSLVWQTEGLVELFVCEHYERAVCEGTP
                     AYFTFSDQKGAETLIKKRWGKGLLYPRAEQEAMAAYTCQQAALINTSLDKTKGELSQL
                     TPELRDQVAQLDAATHRLVIPWNIVVYRYVYETFLRDIGVSHADLTSYYRNHQFDPHI
                     LCKIKLGTRYTKHSFMSTTALKNGAMTHRPVEVRICVKKGAKAAFVEPYSAVPSEVEL
                     LFPRGCQLEVVGAYVSQDHKKLHIEAYFKGSL"
     gene            22021..22503
                     /locus_tag="VHD02_000983"
     CDS             22021..22503
                     /locus_tag="VHD02_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497601.1"
                     /translation="MKTKSKRFLNLATLCLALLSTTLLVTQPVKAETVLTQSSAGGSN
                     GSEKNSEQQRDILKKRYMDRTNASLEELEQDRFEGYFNGYNDGLNGRERPERDNIYVP
                     SGYGDGNGDVEYRDGYQEGFEEGRHEGHPVTAFLEDLWGFLTDIFGNLFGSNNSSQSQ
                     "
     gene            22999..23433
                     /locus_tag="VHD02_000984"
     CDS             22999..23433
                     /locus_tag="VHD02_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497602.1"
                     /translation="MKTKSKRFLNLATLCLALLGTTLLMGRPVNAEVTYTGERSTSVN
                     GPGEDSSSEKTEQTSDSDKRDRNRGYEEGYKRGQESDNRVLDRGTIEVPYNVIDGVEY
                     KDGYEQGFAAGWYDGHPITALLDIAWSYLTYAFDVLFGGTNS"
     gene            23876..24289
                     /locus_tag="VHD02_000985"
     CDS             23876..24289
                     /locus_tag="VHD02_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497603.1"
                     /translation="MTKAIKLVILSLILFTTVFLSVRQASADLMMDGVTYSESTDSNT
                     FLSNSGEVDNIWEKDRDFGKQRGLEAGKNGLGPTINRGDIPVPEKVKHKDKYQDGYQE
                     GYIQAWHQEHPFQGVIFAVFDVFSLIWGVISELFT"
     gene            24530..24781
                     /locus_tag="VHD02_000986"
     CDS             24530..24781
                     /locus_tag="VHD02_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497604.1"
                     /translation="MPEKKVLPVLSMVVSLVSLFDHIFFFATYSYLYLPLVASSLALI
                     GLVQNKGRQKVWSLAGLILAALAVAVVAYAYYDPTVIPY"
     gene            complement(24875..25507)
                     /locus_tag="VHD02_000987"
     CDS             complement(24875..25507)
                     /locus_tag="VHD02_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497605.1"
                     /translation="MKKTLTLLLALFAIGVTSSVRAEDEQSSKQKPVKFDLDEPQQKI
                     KDYSGNTITLEDLYVGSKVVKIYIPQGWWVYLYRQCDHNSKERGILASPILEKNMTKT
                     DPYRQYYTGVPYILNLGEDPLKKGEKLTFSFKGEDGFYVGSYIYRDSDTIKKEKEAEE
                     ALQKKEEEKQQKQLEESMLKQIREEDHKPWHQRLSESIQDQWWNFKGLFQ"
     gene            complement(26019..>26413)
                     /locus_tag="VHD02_000988"
     CDS             complement(26019..>26413)
                     /locus_tag="VHD02_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3644 domain-containing protein"
                     /protein_id="HEQ5497606.1"
                     /translation="RQNLYITKKKDEADFVVSISKDAETPVSLVKELKDPSDTHKYSY
                     SNVVTAVQERLKKSAIKLGYEKGFNTYVLNLVIDFYNIKSETKFSYCHLIGSTQTYTY
                     SQQFVEFIVNEIKKDPQNFVESLKKSNK"
BASE COUNT         8198 a         5322 c         5515 g         7378 t
ORIGIN      
        1 actgcaaaga tatgccagtg ctttttagct cattttttga taaaatagaa ctactaaaaa
       61 gtgaaggaaa taagatgatc acaggaaaac tcataaccgt tgaaggtcca gatggcgcag
      121 ggaaaacgac tgttttagag caactcattc cactgttaaa acaaaaagtg gctcaggaga
      181 tcttgacaac aagagagcct ggaggtgttg ccatttcaga atacattaga gaacttattt
      241 tagacatcaa ccatactact atggacccta aaacagaact cttactttat attgcggctc
      301 gaaggcagca tttggtggaa aaggtactac cagctcttga agcaggccag ttggttttta
      361 ttgatcgttt catcgatagt tcagtagctt atcaaggtgc aggacgcggt ttgatcaaag
      421 cagacattca gtggctcaac gagttcgcaa cagatggttt agagcctgac ttgacccttt
      481 attttgatgt tccttctgaa attggtttag cacggattaa cgcgaaccaa caaagagaag
      541 tcaatcgttt agatttggaa acaatagaga tacatcaacg agtacgaaaa ggatacctag
      601 ccttagctaa ggagcatcca aaacgtattg tcacaattga tgcgactaaa ccattgaaag
      661 aagttgtctc tgtcgctttg gaacatgtct tagccttgct tttagcctag gataagggag
      721 ggtaggaatg gatttagcgc aaaaagctcc taacgtttat caagcttttc agacgatttt
      781 aaagaaagac cgtctgaatc atgcttatct tttttcgggt gattttgcta atgaagaaat
      841 ggctcttttt ttagctaagg tcatcttttg tgaacagaaa aaggatcaga agccctgcgg
      901 gcattgtcgc tcttgtcaat tgattgaaca aggagatttt gccgatgtga cggtattgga
      961 accaacaggg caagtgatta aaacggatgt ggtcaaagaa atgatggcta acttttctca
     1021 gacaggatat gaaaacaaac gacaagtttt tattatcaaa gattgtgaca aaatgcatat
     1081 caatgccgct aatagcttgc taaaatacat tgaggagcct cagggagaag cttacatatt
     1141 tttattgacc aatgatgata acaaagtgct tccgaccatt aaaagtcgga cacaggtttt
     1201 tcagtttcct aaaaacgaag cctatcttta ccaattggca caagaaaagg gattattaaa
     1261 ccatcaggct aagttagtag ccaaacttgc cacaaatacc agtcatctag aacgtctgtt
     1321 gcaaacgagc aagcttttag aactgataac tcaagcagag cgttttgtat ctatttggct
     1381 gaaagatcag ttgcaggcat atttagcgtt gaaccgtctg gtacagttag caactgaaaa
     1441 agaagaacaa gatttagttt tgaccctttt gaccttgctc ttggcaagag agcgtgcgca
     1501 aacgcctttg acacaattgg aggctgtcta tcaggctagg ctcatgtggc agagcaatgt
     1561 taattttcaa aacacattag aatatatggt gatgtcagaa taagtgcaat tggaattcgt
     1621 tatggggaaa cgaagcttgt caaaagactt gcccgtagcg accaaagtag cggcgcatag
     1681 gggcaaaaca agcttttgaa gaaaatactg cctttgcaac agatttgatg aaagaagtat
     1741 catgattaat aacacaaaat caacttaaaa tgacgtttat taatagtgtc tttcccttag
     1801 atcagcatgt tgttctaatt cccttttcag cagaagagca catggatttt tacaactgtt
     1861 aaaagattta gcggccgatt ttaggtcaag aagtgagctg ccccagatta acagccaaga
     1921 agatttcatt ttctatggag gctatggacc gtgtggcagg ccactgtgct gctttcgttt
     1981 ttgggaaaat ttctgacagt atctattaaa atactaaaaa atcaagggtt atcattgact
     2041 ttgggtaaag caacaagccc ttatggccgt ttgctttgct gtctctatga tgaaggtggc
     2101 ttttaccaat aaagcaagaa aaaattccct gaatatggta ccatagtaga gacaagtgat
     2161 ggccctgcaa cagtagcagc cgttgatata tttacagatc tcattaaggt gcggttaaaa
     2221 gaccagttga ccttagtcac ttatgcttta gaggaggtta aagtaggtga ataaaaaaga
     2281 attattcgat gcttttgatg gattttccca aaatttaatg gttactttag ctgaaattga
     2341 agccatgaaa aaacaagtcc aatcactagt agaagaaaac acgattcttc gcctagaaaa
     2401 tacaaaacta cgggaacgtt tgagtcacct agaacatgaa acggtagcta aaaatccttc
     2461 caaacaaagg aaggatcact tagagggaat ttatgatgaa gggtttcata tctgcaattt
     2521 tttctatgga caacgtcgtg aaaatgatga agaatgtatg ttttgcagag aattattaga
     2581 tagaaagtaa gggcatgcag gttcaaaaaa gttttaaaga caaaaaaaca agcggaacct
     2641 tgtatttggt tccaacccca attggcaatc tgcaggacat gacctttaga gcagtggcaa
     2701 cgttaaaaga ggttgatttt atttgtgcag aagacacaag aaacacaggt cttttgctaa
     2761 aacactttga cattgctacc aagcaaatca gctttcatga gcacaacgcc tatgagaaga
     2821 ttcctgattt aattgattta ttaatatcag gacggtcact agctcaggta tctgatgcag
     2881 gaatgccatc gatttctgat cctgggcatg atttggtcaa ggctgctatt gatagtgata
     2941 tcgcagtcgt tgctttacca ggcgcctcag cagggattac agctttgatt gctagtggac
     3001 tggctcctca accccatgtt ttttatggat ttttacccag aaaagcaggg caacaaaagg
     3061 ctttctttga ggacaaacac cattacccag agacacaaat gttttatgaa tcgccctatc
     3121 gcatcaaaga taccctgacc aatatgttgg cttgttatgg tgatcgtcaa gtggtacttg
     3181 ttagagaatt aaccaagctt tttgaggagt accaacgggg gtcgatttta gaaatcttgt
     3241 cctaccttga ggaaacaccc ctaaaagggg agtgtctgct gattgtggct ggagcccaag
     3301 tagacagtga ggttgagctg acagctgatg ttgacttagt gtctctagtg caaaaagaaa
     3361 tccaagcagg agccaagcca aatcaggcta ttaaaacaat tgctaaagct taccaggtaa
     3421 atcgccaaga gctttaccaa caatttcatg acttataagt catcgtagat taggagaaaa
     3481 acaaatgaca gaagatcaag caactatcct tattttagga gtatacggaa tcatattggc
     3541 tgtattctta ttatcagcct tattatgcct tatcgctaac tggcgactct ttaaaagagc
     3601 aggttacaaa ggatggtata gtctcattcc tttctatgca ggttttgtca aacacaaaat
     3661 cacttttgga gaagataata aatggtttta ctttattggt tgggtcattg ctatttatta
     3721 ttactatgtg cacttttgct atgtccgtgc ttttggtgga tcaaagggtc tagcagtatt
     3781 tggcttgttt ttcccaggca ttgcttcttt gatagtggct tttggaaaaa cttataccac
     3841 acaagaatgc caccctgtgc cacatctttt aaactaagag aaaaaagacc atcaattgga
     3901 tttattgatg gtcttttagg tgtttaaatg attttagtcc cgtgaacaat ggccgttcca
     3961 gtgagttgac tcaagttttg gcaattctca gcagtgacgc ctcctccaat catgatttca
     4021 atgcgtttat tggcataggt cacaagactt ttaagctgtt ccacattatc tgtaatagga
     4081 gtagcttcag gggagccatg agttagcaca cgcacaaagc cataatcaat cagttgatca
     4141 agcgcttggt gctggtggtc agtcggaata tggtcaaaag ccatgtggaa aaccaatggc
     4201 aagccttgtg tagcaggcag taactgctcg atagcgtcgg tatcaaggtg attttctgtg
     4261 gtcagcaagc ctaagactag cgcatctgag ccagcttcaa cagcttttaa gatatcttct
     4321 tccatagctt ttagttcgag gtcgttatag acaaaatcgc caccgcgagg tcgaatcatg
     4381 gtcgctacgc taatcttttt atcgtgtaag agctgacagg cttctttgat gacgccataa
     4441 gatggtgttg ttcccccgac agctaggtta tcacagagtt ccacacgact gacttgacca
     4501 gcatcaaggg ttggcagtag tgtgaggttc tcagcacaaa attctttaat catttttaga
     4561 tctcctttat cactaatatt atagcacgaa tgaagctagt catcaggaaa catttttgtc
     4621 aaatcatgga cattatgtta tactcactag tattaaaagt aggccaaaac agcagcaaat
     4681 actttgaaaa aagattgcta tgttctacgg caatatcagt ctttaccaac gaactctcaa
     4741 gaagaaaaga aagacaaagc ttagcattta aaaaaagccc cccttttttt gtgaaatgat
     4801 gaccaaaaac aggcgcctca cgcctaaaac agttacccta aaggagaaca aatgattacg
     4861 atttaccaat acccaaaatg tagtacctgt caaagagcca tggctgaact aaagcaactg
     4921 gttagtgact ttgaagtcat tgacatcaaa gctaatccac ccaaagctca ggacctcaaa
     4981 cattggatgg aaacatctgg ttataccatc aaaaatttct ttaataccag tggtaacagc
     5041 taccgcgaat tgggacttaa agataaaata gaccagctta gccttgataa agctgctgaa
     5101 ctcttagcca cagatggtat gctgattaaa cggcctattc tgattaagga cggtagagtc
     5161 ctacaagttg gctatcgcaa gccatatcaa gagttggatt taggataatg cgacaaagtc
     5221 tcaaaaaagg acaaggcaac attataacgc cttgtccttt tttgggatct gtttttagaa
     5281 attagagatc aatatctagt aaaattggcg tatggtcttg gcgttcccca gaagaaatca
     5341 tttcagaacg cttaacctta tccacaagtc ggtttgaagc tagccaatag tcaattctcc
     5401 aaccggtatt attgattttg ctggttttgc tacgttgtgc ccaccaggta taaacatgag
     5461 gaatatcgcc gtgcaagtga cgaaaagtat ctgtaaagcc tcttgctaat aggttagtaa
     5521 agccttgacg ttcttcgtcg gtaaaaccag gggaacgtcg attgctgctt ggatttgcca
     5581 aatcaatctc tttgtgggca acgttgtaat ctcccgtagc caaaacaggc ttttgagcat
     5641 ccagttcagt caagtaatca gcgtatttgt ggtcccaaat ttgacggtca tctaaacgtc
     5701 taaggccatc accagcatta ggcgtgtaaa cctgtgtgac gaaaaagtct tcaaattcta
     5761 gggtaatgat acggccctca gcatccattg ttgttggggc accaatttct ggaaaagtaa
     5821 tgacagggtt tagcgtgttt ttgtacagga acatggtgcc agcatagcct ttcctagcag
     5881 gctcaactga agaacgccac acatgcaaat agtttggaaa gtaactaagt aaggtctcga
     5941 tatgtttttt ggtaggcccc ttagcagata atttggtttc ttgaatggca ataatatccg
     6001 catcttgagc gactaaagtg tctaaaacag cgcgtgataa aagcgcacga ggggactctc
     6061 ctgtgagagc agcatttaag gaatcaatgt tccatgaaat cagtttcatt tggtatctct
     6121 ttctaacaga tagtcttgtt gttattgtaa caaaaaacga aacgataagc gactttggac
     6181 acctagcccc cataaaacag tctagccaaa tgaagcatag cagcgtaaga acaagataag
     6241 gaggtgatca gtgagcatat cgtttgacag ttaagagtag gattactata atagaagcat
     6301 aaatgtaagc gctaacaaag taaaaaaata taggagaatg tcaatggcac aaaaaacagt
     6361 catcaccgag gaaacaaccg attttgttat ggatttcaaa acaagtagcg ctgagggaaa
     6421 tgttgatttt attaacgttt ttgaccttga gaaaatggca caacaggtca tccctaaagg
     6481 agcctttggt tatattgcta gtggtgctgg tgataccttt accctccatg aaaatattcg
     6541 ctcatttaat cataaattaa ttgtccctca ctccctcaaa ggggttgaaa atccgtcaac
     6601 agagattacg tttgatggtg atcacttaac ctctcctctt attttagcac ctgtagcggc
     6661 tcataagctg gccaatgaac aaggagaagt agcgtctgcc aaaggattga aggaatttgg
     6721 aagtatctac accactagtt cctactcaac aactgacctg ccagaaatca gtgctgcctt
     6781 aggtgggaca ccccattggt ttcaatttta ttacagtaaa gacgatggca ttaatcgtaa
     6841 tattatggat cgtgtcaaag cacaaggttg caaagctatc gttttgacag cagatgcaac
     6901 agttggagga aaccgcgaag ttgataggcg aaacggcttt gtttttccgg tcggtatgcc
     6961 aattgttcaa gaatatttac ctgatggtgc aggtaaaaca atggattatg tctataaatc
     7021 agctaaacaa gcgcttacct caaaagatat tgaatatatc gcaacttact caggcctgcc
     7081 tgtttatgtc aaaggaccac agtgtgctga ggataccttg cgagcattgg atgcaggagc
     7141 atcaggtatc tgggtaacca atcatggtgg ccgccaatta gatggtggtc cagctgcttt
     7201 tgattcctta caagaagtcg ctgaagcagt tgatcaaaaa gttcctattg tctttgactc
     7261 aggtataaga cggggtcaac atattttcaa agctcttgct tcaggtgctg accttgttgc
     7321 cttggggcgt ccagcgattt acggcttggc tatgggagga agcactggta ctcgtcaggt
     7381 ctttgaaaag ttaaatgacg agttaaaaat ggtcatgcaa ctggctggga cacagactat
     7441 ccaagatgtt aaagccttta accttcgcca caatccgtat gattcatcga ttccatttga
     7501 tcaaaatgcg ctacgcttag attagaacag acaaaaacct ctgctatcat gtcacaaata
     7561 gtagaggttt tacactgcaa aaagaggtat gaaaattcaa taacatagtt acaaggctat
     7621 catttaccac ccccactaat agattaaaat gaaagacttc ttacattaat agaagtttct
     7681 gttgcttttc tcagaaaaat gtggttaaat aatcaaatat tattaacgaa aaatgagtta
     7741 atgattaaca ttaaactggt tttagttaaa acatttagga gggtatcagg tggagaaaaa
     7801 gcaacgtttt tcccttagaa aatacaaatc aggaacgttt tcggtcttaa taggaagcgt
     7861 tttcttgatg atgacaacaa cagtagcagc agatgagcta accacaacga gtgaaccaac
     7921 aatcacgaat cacactcaac aacaagcgca acatctcacc aatacagagt tgagctcagc
     7981 tgaatcaaaa cctcaagaca catcacaaat cactctcaag acaaatcgtg aaaaagagca
     8041 accacaaggt ctagtctctg agccaaccac aactgagcta gctgacacag atgcagcacc
     8101 aatggctaat acaggtcctg atgcgactca aaaaagcgct tctttaccgc cagtcaatac
     8161 agatgttcac gattgggtaa aaaccaaagg agcttgggac aagggataca aaggacaagg
     8221 caaggttgtc gcagttattg acacagggat cgatccggcc catcaaagca tgcgcatcag
     8281 tgatgtatca actgctaaag taaaatcaaa agaagacatg ctagcacgcc aaaaagccgc
     8341 cggtattaat tatgggagtt ggataaatga taaagttgtt tttgcacata attatgtgga
     8401 aaatagcgat aatatcaaag aaaatcaatt cgaggatttt gatgaggact gggaaaactt
     8461 tgagtttgat gcagaggcag agccaaaagc catcaaaaaa cacaagatct atcgtcccca
     8521 atcaacccag gcaccgaaag aaactgttat caaaacagaa gaaacagatg gttcacatga
     8581 tattgactgg acacaaacag acgatgacac caaatacgag tcacacggta tgcatgtgac
     8641 aggtattgta gccggtaata gcaaagaagc cgctgctact ggagaacgct ttttaggaat
     8701 tgcaccagag gcccaagtca tgttcatgcg tgtttttgcc aacgacgtca tgggatcagc
     8761 tgaatcactc tttatcaaag ctatcgaaga tgccgtggct ttaggagcag atgtgatcaa
     8821 cctgagtctt ggaaccgcta atggggcaca gcttagtggc agcaagcctc taatggaagc
     8881 aattgaaaaa gctaaaaaag ccggtgtatc agttgttgta gcagcaggaa atgagcgcgt
     8941 ctatggatct gaccatgatg atccattggc aacaaatcca gactatggtt tggtcggttc
     9001 tccctcaaca ggtcgaacac caacatcagt ggcagctata aacagtaagt gggtgattca
     9061 acgtctaatg acggtcaaag aattagaaaa ccgtgccgat ttaaaccatg gtaaagccat
     9121 ctattcagag tctgtcgact ttaaaaacat aaaagatagc ctaggttatg ataaatcgca
     9181 tcaatttgct tatgtcaaag agtcaactga tgcgggttat aacgcacaaa acgttaaagg
     9241 taaaattgct ttaattgaac gtgatcccaa taaaacctat gacgaaatga ttgctttggc
     9301 taagaaacat ggagccctgg gagtacttat ttttaataac aagcctggtc aatcaaaccg
     9361 ctcaatgcgc ctaacagcta atgggatggg gataccatct gctttcatat cgcacgaatt
     9421 tggtaaggcc atgtcccaat taaatggcaa tggtacagga agtttagagt ttgacagtgt
     9481 ggtctcaaaa gcaccgagtc aaaaaggcaa tgaaatgaat catttttcaa attggggcct
     9541 aacctctgat ggttatttaa aacctgacat tactgcacca ggtggcgata tctactctac
     9601 ctataacgat aaccactatg gtagccaaac aggaacaagt atggcctctc ctcagattgc
     9661 tggcgccagc cttttggtca aacaatacct agaaaagact cagccaaact tgccaaaaga
     9721 aaaaattgct gatatcgtta agaacctatt gatgagcaat gctcaaattc atgttaatcc
     9781 agagacaaaa acgaccacct caccgcgtca gcaaggggca ggattactta atattgacgg
     9841 agctgtcact agcggccttt atgtgacagg aaaagacaac tatggcagta tatcattagg
     9901 caacatcaca gatacgatga cgtttgatgt gactgttcac aacctaagca ataaagccaa
     9961 aacattacgt tatgacacag aattgctaac agatcatgta gacccacaaa agggccgctt
    10021 cactttgact tctcgctcct taaaaacgta ccaaggagga gaagttacag tcccagctaa
    10081 tggaaaagtg actgtaaggg ttaccatgga tgtctcacag ttcacaaaag agctaacaaa
    10141 acagatgcca aatggttact atctagaagg ttttgtccgc tttagagata gtcaagatga
    10201 ccaactaaat agagtaaaca ttccttttgt tggttttaaa ggacaatttg aaaacttagc
    10261 agttgcagaa gagtccattt acaggttaaa atctcaaggc aaaactggtt tttactttga
    10321 tgaatcaggt ccaaaagacg atatctatgt cggtaaacac tttacaggac ttgtcactct
    10381 tggttcagag actaatgtgt caaccaaaac gatttctgac aatggtctac acacacttgg
    10441 cacctttaaa aatgtagatg gcaaatttat cttagaaaaa aatgcccaag gaaaccctgt
    10501 cttagccatt tctccaaatg gtgataacaa ccaagatttt gcagccttca aaggtgtttt
    10561 cttgagaaaa tatcaaggct taaaagcaag tgtctaccat gctagtgaca aggaacacaa
    10621 aaatccacta tgggtcagcc cagaaagctt taaaggagat aaaaacttta atagtgacat
    10681 tagatttgca aaatcaacga ccctgttagg cacagcgttt tctggaaaat cgttaacagg
    10741 agctgaatta ccagatgggt attatcatta tgtggtgtct tattacccag atgtggtcgg
    10801 tgccaaacgt caagaaatga catttgacat gattttagac cgacaaaaac cggtactatc
    10861 acaagcaaca tttgatcctg aaacaaaccg attcaaacca gaacccctaa aagaccgggg
    10921 attagctggt gttcgcaaag acagtgtctt ttatctagaa agaaaagaca acaagcctta
    10981 tacagttacg ataaacgata gctacaagta tgtctcagta gaagacaata aaacatttgt
    11041 ggagcgacaa gctgatggca gctttatctt gccgcttgat aaagcaaaat taggggattt
    11101 ctattacatg gtcgaggatt ttgcagggaa cgtggccatc gctaagttag gagatcacct
    11161 accacaaaca ttaggtaaaa caccaattaa acttaagctt acagacggta attatcagac
    11221 caaagaaacg cttaaagata accttgaaat gacacagtct gacacaggtc tagtcacaaa
    11281 tcaagcccag ctagcagtgg tgcaccgcaa tcagccgcaa agccagctaa caaagatgaa
    11341 tcaggatttc tttatctcac caaacgaaga tgggaataaa gacttcgtgg cctttaaagg
    11401 cttgaaaaat aacgtgtata atgacttaac ggttaatgta tacgctaaag atgaccacca
    11461 aaaacaaacc cctatctggt ctagtcaagc aggcgctagt gcatcagcta ttgaaagtac
    11521 agcctggtat ggcataacag ctcgaggaag caaggtgatg ccaggtgatt atcagtatgt
    11581 tgtgacctat cgtgacgaac atggtaaaga acatcaaaag cagtacacca tatctgtgaa
    11641 tgacaaaaaa ccaatgatca ctcagggacg ttttgatacc attaatggcg ttgaccactt
    11701 tactcctgac aagacaaaag cccttggctc atcaggcatt gtccgcgaag aagtctttta
    11761 cttggccaag aaaaatggcc gtaaatttga tgtgacagaa ggtaaagatg gtatcacagt
    11821 tagtgacaat aaggtgtata tccctaaaaa tccagatggt tcttacacca tttcaaaaag
    11881 agatggtgtc acactgtcag attattacta ccttgtcgaa gatagagctg gtaatgtgtc
    11941 ttttgctacc ttgcgtgacc taaaagcggt cggaaaagac aaagcagtag tcaactttgg
    12001 attagactta ccggtccctg aagacaaaca aatagtgaac tttacttacc ttgtgcggga
    12061 tgcagatggt aaaccgattg aaaacctaga gtattataat aactcaggta acagtcttat
    12121 cttgccatac ggcaaataca cggtcgaatt gttgacctat gataccaatg cagccaaact
    12181 agagtcagat aaaatcgttt cctttacctt atcagctgat aataacttcc aacaagttac
    12241 ctttaagatg acgatgttag caacttctca aataactgcc cactttgatc atcttttgcc
    12301 agaaggcagt cgcgttagcc ttaaaacagc tcaaggtcag ctgatcccgc ttgaacagtc
    12361 cttgtatgtg cctaaagctt atggcaaaac cgttcaagaa ggcacttacg aagttgttgt
    12421 cagcctgcct aaaggctacc gtatcgaagg caacacaaag gtgaataccc taccaaatga
    12481 agtgcacgaa ctatcattac gccttgtcaa agtaggagat gcctcagatt caactggcga
    12541 tcataaggtt atgtcaaaaa ataattcaca ggctttgaca gcctctgcca caccaaccaa
    12601 gacaacgacc tcagcaacag caaaagccct accatcagcg ggtgaaaaaa tgggtctcaa
    12661 gttgcgcata gtaggtcttg tgttactcgg acttacttgc gtctttagcc gaaaaaaatc
    12721 aaccaaagat tgataacaaa acgcaaagag acaacaaatc atccctttta acagtcggac
    12781 ctttctttga gattgagctg gcagctgatc atttcttaac aaaagttaaa gagttgagca
    12841 aagtaaggaa caaacataaa gaccataaaa gcaagccaaa ctggcttgct tttatggtgt
    12901 tattgtcgtg tgctacgatg actaaaaaag aacaacgaac aatgacctcg aatcaccaaa
    12961 aggacttgat ttgatagctt ccctatgcta tactaagcct attctttcaa gaaggactcc
    13021 ttttgtggaa aagactgaaa aaaaggacac taatctatcc aaaaactcca gatgagtagc
    13081 tgcaaacgga tgcctttgct gtgatcaagg tgataaagca gactggcttg tcactacctg
    13141 agcaggtatc aaaaaaagaa gcttttggct ttgtcattca acatactgag ctttagtatt
    13201 ttagaacgga gacaccccca gttttgtact caaaaagcat aaacagctct gctgatgtga
    13261 catccgattt ctacgtgcgc agtgatccaa aacagctgct gtttgctatg gtaaaagtgg
    13321 cgccaatcgg gatttttgag accctaggct caaaaataag gcatagaatt ccaaaggaag
    13381 tcgctgccgt gagcacaacc taagaaaaac tatcccaaaa gactttttct gcaatttgaa
    13441 atactgaatt tcagtatttt ttcttttgta actaagagca atgaatgagg taaaaaaggc
    13501 aacaatgtca aaaaagaaac gaaaacacta tcaggtttat gagggattac gctgtgctat
    13561 gacgctttgt tttatcagtg gctatgtcaa tgcctttacc tatatgacac aaggcaaacg
    13621 ttttgcaggt gttcagacag gcaacctttt gtcatttgcc attcgcttgt cagaacagca
    13681 actgaaggag gcactgcaat ttttacttcc catgatagtt tttatgcttg ggcagtcctt
    13741 tacttatttc atgcaccgct gggccattaa aaaagggctc cactggtact tattatcaag
    13801 ctttatctta acagggattg cttttggaac ggccctattc acgccttttc ttccttccaa
    13861 tgttacggta gcagctttag ccttttttgc ctctattcag gttgatacgt ttaaaacctt
    13921 acgaggagct agctatgcta atgtgatgat gacaggaaat atcaaaaatg ccgcctattt
    13981 actaaccaaa ggcttgtacg aaaaaaatca cgaactaaca catatcggac gcaatacctt
    14041 aatagtcatc ttggcttttg ctgttggggt ggtttgttcg acgttgcttt gcatagctta
    14101 tggcgagtat gctttgatgc ctattttaat gcctttactc tatgtcaatt acctgttggc
    14161 tcaggaattt tatcacattc aaaccaaaat caaaccgatt aaaagaactt aacggacttg
    14221 tggagaaaga aagttcattt agggtaagtt tttacttacc ctccgttgct ttgctggttt
    14281 tgctgcccct taaaatatga tataatagac cattaatagc tgttacttaa cagaaggctg
    14341 ataaaaaagg ttccattagc cttgagggtt gaatttaaac aggaaaaaac gaaatcctac
    14401 actatgcatc tcaatcgtac tttcatttga gtatggttct gaaaggaaac tatgatgaaa
    14461 aaaccatttt acattacgac acccatttat tacccatctg ggaaattaca cattggttct
    14521 gcctatacaa ctattgcttg tgatgtcttg gcacgctaca agcgcttgat gggccatgag
    14581 gtcttttacc tcacaggact tgatgagcac ggacaaaaga ttcaaaccaa ggcaaaagaa
    14641 gctggtatta cacctcaaac ctatgttgat aacatggcaa aggatgtcaa agccctttgg
    14701 caattgctag atatttccta tgacacattt atccggacaa cggacgatta ccacgaagag
    14761 gtcgttgctg ctgtctttga aaaattgctg gcgcaagacg acatttacct tggcgaatac
    14821 tctggttggt attctgtctc tgatgaggaa tttttcactg aaagccaatt aaaagaagtc
    14881 tttagagatg aagacggtca ggttattgga gggattgccc catcaggtca tgaggtcgag
    14941 tgggtctctg aagaatccta tttcctacgc cttagcaaat acgctgatcg tcttgtggcc
    15001 ttctttaaag agcgacctga ctttatccaa ccagacgatc gtatgaatga aatggtgaaa
    15061 aactttatcg agccaggtct agaagatttg gctgttagcc gtactacctt tacatggggt
    15121 gtacctgttc catcagaccc aaaacacgtg gtttatgtgt ggattgatgc cctcttaaac
    15181 tacgcgacag ctcttggtta tggtcaagct aatcacgcta actttgataa attctggaat
    15241 ggaacggttt tccacatggt tggaaaagat atcttgcgtt ttcatagcat ctactggcca
    15301 attttactca tgatgcttga tttaccaatg ccagaccgct taattgctca tggttggttt
    15361 gtcatgaaag acggtaaaat gtctaaatca aaaggcaatg tggtctatcc agaaatgtta
    15421 gtagagcgct ttggactgga ttctcttcgt tattacctca tgcgttcttt gccagttggt
    15481 tcagacggga cctttacccc tgaagattat gtgggtcgta tcaactatga gttagccaac
    15541 gaccttggca atttattgaa ccgtaccgtt gcgatgatca acaagtattt tgatggcacg
    15601 gtgccagctt atgttgacaa tggaactgcc tttgatgctg acttaagtca actgattgat
    15661 gctcagctag cagactacca taagcacatg gaagctgtag actacccacg cgccttggag
    15721 gctgtttgga ccatcatcgc tcgtaccaac aaatacatcg atgaaaccgc tccttgggtg
    15781 ctcgctaaag aagatggtga caaggctcaa ctagccagtg tcatggcaca cttggcagct
    15841 agcttgcgcg tggtcgctca tgtgatccaa ccctttatga tggaaacatc agctgctatc
    15901 atggcccagc ttggcttagc gcctgtttct gacttgtcaa cacttgctct agcagatttt
    15961 cctgccaata caaaggtcgt tgccaaaggc acaccgattt tcccacgtct tgatatggaa
    16021 gctgagattg actacatcaa agcccaaatg ggtgacagct cagctatttc ccaagaaaaa
    16081 gaatgggtcc ctgaagaggt tgcgctcaaa tctgaaaaag acgtcatcac atttgaaacc
    16141 tttgatgctg tggaaatccg tgtggcagaa gtcaaggaag tgtcaaaagt ggaaggttca
    16201 gaaaaattgc tgcgctttag agtagatgca ggagacgggc aagaccgtca aatcttgtca
    16261 ggtattgcca aattttaccc caacgaacaa gaattagtgg gtaaaaaact acaaattgtt
    16321 gctaacctga aaccgcgtaa aatgatgaaa aaatacatca gccaaggcat gattctatca
    16381 gcagagcatg gagatcaact aacggtctta accgttgatt cctctgttcc aaatggaagt
    16441 atcattggct gatgctgacc tattattcaa aaaacctaag caagaacatg tgcttaggtt
    16501 tttttgattg ccttcaagaa ggacgaataa cgagatgggt gatatggcat caaaagcata
    16561 actatcctag gacctgtcct aggatagtta tgcttttgac aagtgaacag agaggtctta
    16621 aaactatatc ttgtcataaa aaagattgca aaacactata tcttgtgtta ttatggtttt
    16681 tataaaagtt aagtatgctt aactttagcc aaacaaaaga ggacactaga gcgtgtccca
    16741 caagacagtt gagcccttcg cttttttagt agagtaggag tgctcaagtc aaaaagagac
    16801 atcccatcaa accattgctg tttagcaggt ctttttgtgt tgctcaaaaa aaaaacaatt
    16861 cctgtctgtt tggcgtctag tgatgactta ggcttagcgc tactagactt ttgtttgaaa
    16921 aatacctcaa gccttgaagg agacaaatga cccaacatta ttatgaacgt tcgcaatccc
    16981 caattgaata cgccctatct gaaacacaaa aacagttgcg ctccatcaat tggaattacc
    17041 ttaatgatga caaagatttg gaagtgtgga atcgtgtcac tcaaaatttt tggttgcctg
    17101 aaaaggttcc tgtttctaat gatttaaatt cttggcgctc cttgggtgag gattggcaac
    17161 agctcatcac aaggacctac actgggttga ccttactaga tacagtacaa gcaacggttg
    17221 gtgatgtggc acaaattcag cattcacaaa ccgaccacga acaagtcatc tatacgaact
    17281 ttgcctttat ggtggggatt catgcccgct cttacgggac gattttttcg accctttgca
    17341 gcagcgagca aattgaagaa gcacatgaat gggtggtctc aacacagagc ttgcaagacc
    17401 gtgcccgcgt cttgattcct tactatacgg gtgatgaccc tttgaaatca aaagtagcag
    17461 cagctatgat gccaggattt ttgctgtatg gcggctttta ccttcctttt tacctatcag
    17521 cacgtggtaa aatgccaaac acatctgata tcatccgcct gattttacgc gacaaagtta
    17581 ttcacaacta ttacagcggt tacaaatacc aacaaaaagt agcacgtctt agcccagaaa
    17641 aacaggctga aatgaaggcc tttgtctttg acttgctcta tgaactcatc gaccttgaaa
    17701 aagcttactt gagagaactg tatgcaggct ttgatttggc agaagatgct atccgcttta
    17761 gcctttataa cgctggaaaa ttccttcaaa atttgggata cgagtcgccc ttcacagatg
    17821 aagaaacgcg cgtttcacca gaagtttttg ctcagctatc agcgcgtgca gacgaaaacc
    17881 atgacttttt ctcaggcaat ggctcatctt atgtgatggg gatcaccgaa gaaaccacag
    17941 atgacgattg ggagttttaa aaaatggcag agctgattat tgtgtatttt tcatctaagt
    18001 ccaataacac ccatcgcttc gtccaaaaat taggcctccc tgctcagcgc ataccagtag
    18061 ataataggcc acttgaagtt tcaacccatt accttttgat tgtgcctacc tatgcagctg
    18121 gtggctcaga tgccaaagga gccgttccta aacaagtgat tcgttttttg aataatccta
    18181 acaatcgtaa acattgtaag ggagtgatct cttcaggaaa taccaatttt ggcgatacct
    18241 ttgccttagc aggccctatc atttctcaaa aattgcaggt ccccctatta caccaatttg
    18301 aactattagg cactgctaca gatgtcaaaa aggtccaggc gatttttgcc cgcttaaagc
    18361 atcacacaca cgacaagcaa aaacaaacca acaacctgat tacagaaagg acacacccat
    18421 gtcacaaacc aatgcgtcat acctctcatt aaatgcactg actcgtttta aaaaaccaga
    18481 cggcagctac cattttgata gcgataaaga agctgttagg cgctacctgg aagaacatgt
    18541 ctcacccaat caaatggctt ttaactcact agaagataag ctagcttacc tcatcaatga
    18601 agggtattat gagcaggcca tttttgatgc ctatcctaac gaccttatca aggaggcctt
    18661 tcattatgcc tatcaacaag gctatcgctt tttaaatctc atgggcgcta tgaaatttta
    18721 ccagtcctat gccttaaaaa ccttggacgg taagcagtat ctagaaacat ttgaagaccg
    18781 tgctgtgatg aatgcactct ttttagctga tggagatcaa acctttgtct ttgacgtgat
    18841 tgatgccatc ttgcaccgac gctttcaacc agccacccca acctttttaa atgccggaaa
    18901 aaaacgccgt ggcgaataca tttcgtgcta tcttcttcgt gtcgaagaca atatggaatc
    18961 tatctctcgc gccattagca ccagcttgca actctcaaaa agaggaggcg gagtcgcctt
    19021 gtgcctcacc aacctcagag aaatcggagc accgataaaa gggattgaaa atcaggcaac
    19081 aggcatcgtt cctgtcatga aactactaga ggactctttt tcctatgcca atcaactagg
    19141 tcagcgccaa ggagcaggtg cggtttacct tcatgcccat caccccgagg tcctgacctt
    19201 tttagacacc aaacgtgaaa atgccgatga gaaaattcgc attaagtccc tagcattggg
    19261 acttgtgatc ccagatatta ccttccagtt agccaaagaa aacaaagaca tggctctctt
    19321 ttcaccctat gatatcaagc gtgtctatgg caaggacatg tcagacatct ctatcacaga
    19381 ggaatacgat aaactgctag ccaatccagc catcaaaaag acctacatca gtgctagaaa
    19441 gctctttcag ttaattgcag agctacattt tgagtcaggc tacccttacc tcttgtttga
    19501 tgacacggtc aataagcgca accctcatgc caaaaaaggc agaatcgtca tgtctaacct
    19561 gtgttctgaa atcgctcaag tcagcacccc aagcactttc aaagaagatt tgagttttga
    19621 aaccattgga gaagatatct gctgtaattt agggtctatc aatattgctc aggctatggc
    19681 agatgcgcca cactttgaac agctcatcac aacaagcatc agagcacttg atcgtgtctc
    19741 tagagtgtct gacctgaatt gtgcgccttc tgttgagaca ggcaatgcag ccaaccatgc
    19801 agtaggatta ggcgccatga acttacatgg gtttttggca accaaccaca tctactatga
    19861 tacaaaagaa gccgttgact ttacagatct gtttttccat gccatggcct atcatgcttt
    19921 taaagcctct tgccaactag ccaaagaaaa aggggctttt gcaggatttt cgttatcaac
    19981 ctatgcagat ggcacttatt ttgccaagta tttgcaagaa gatgccaagc cacaaacagc
    20041 caaggttgcc acattgttgc aagattacgg cttcacattg ccaacggtgg cggactggca
    20101 agccctagta gcagatatca agcagtttgg cctagccaat gcacacttgc tagcggttgc
    20161 tccgacaggc tccatcagct acttatcatc atgcactccg agtctacaac cagtcgttgc
    20221 tcctgttgag gtccgaaaag aaggtagcct gggcaggatt tacgtccctg cctatcagat
    20281 tgaccaggct aactatgctt actatgaacg tggtgcctat gaggtcggcc caaaagccat
    20341 catcgacgtt gtcgctgctg cccaaaaaca cgttgaccaa gccatctcat tgaccctgtt
    20401 tatgaccgat caagcgacca cacgtgacct taaccgctcc tatatccaag cttttaagca
    20461 aaactgtgca tcgatctatt atgtgcgagt gaggcaagac gtcctagcag gcagcgaaca
    20521 gtatgacgag gatagtcttg tgacagctcc tggagctagt gatgagacca ccacagagtg
    20581 tcatagctgt atgatctaac agctactaga aagctctcgt gtcccctacc acatgcatag
    20641 gatcagtgcg ctcgaccttt caagacaagc aagcatcagc tcttgcttgt ctttttttgg
    20701 cctcaaagcc cgttagtctg ctgctatgcg aggctttttt tgagcatcag aacctcaaaa
    20761 aaaggacatg gagtcctttt tggggtgatc ggtgttgagg ctatcacaaa ctgcccttga
    20821 aatacgcttc tatgtggagc tttttgtggt cctgtgacac gtaagctcca acgacctcca
    20881 gctgacagcc tcttggaaac aagagctcaa cctctgaagg cacagccgaa taaggctcga
    20941 caaaggctgc cttggcccct tttttgacac agatgcgcac ctccaccggt cgatgggtca
    21001 tagcgccgtt tttcaaggct gtcgtgctca taaaactgtg cttagtgtag cgtgtgccaa
    21061 gctttatctt acaaaggata tgagggtcaa actgatggtt acggtagtaa gacgtgagat
    21121 cagcgtgtga aacaccgata tcacgcaaaa acgtctcgta tacatagcgg tataccacaa
    21181 tgttccacgg gatgactagc cggtgagtcg cagcatcgag ctgggccacc tgatccctta
    21241 gctcaggcgt gagttggctg agctcaccct tggtcttgtc taggctggta ttgataaggg
    21301 ctgcctgctg acaggtataa gcagccatcg cctcttgctc agcccttggg tagaggagac
    21361 ccttgcccca acgcttttta atcagtgtct cagcgccctt ttgatccgaa aaggtaaaat
    21421 aagcaggcgt cccctcacaa accgcccgct catagtgctc acagacaaaa agctcgacta
    21481 agccctcagt ttgccagacc agactaaaac agctaagaag aaggatcata gccagttgat
    21541 agcgcttttt tagcatagtt ccccctgaca ccagcccaaa aggctgaccc ttcccctagg
    21601 ccttttggct agctcatgat accaccagtt tctcagacag gctaatcttt ttgtcatctc
    21661 tttctcccta aacagactct cttatcatga tattatgcta ttatgagcga tttgtcaatc
    21721 catagcaacc gttttttagt tagataagca agcttttgac ctagaggcag acaagagtgg
    21781 cagtccctac cgtttgctgt ggtggctaat acggactaac acaaaatctc aaacgctttc
    21841 aaaaacagct cctgagctta agcttaaaaa aggctgatga acagatttgt tgaacttaag
    21901 agagattgcc atgattttct aatcatttga tgcgtaaaat gataaaaaca tgaacttgaa
    21961 atgaaagaat gcgagtagta aactaaaaat atgtcaaaaa taatgaaatg gagtgatttt
    22021 atgaaaacaa aatcaaaacg ctttttaaac ctagcaaccc tttgcttggc ccttttgagc
    22081 acaaccttac tagtgacaca gccagtaaag gcggagacgg tgcttaccca gagctcagct
    22141 gggggtagta acgggtcaga aaaaaatagt gagcagcaga gagatatatt aaaaaaacgt
    22201 tatatggatc gaacaaatgc aagcttggaa gagttagaac aagatagatt tgaaggatat
    22261 tttaatggat ataacgatgg attaaacgga cgcgagagac ccgaacgaga taatatttac
    22321 gttcctagtg gttatggaga tggcaatggg gatgttgaat atagagatgg ctatcaagag
    22381 ggatttgaag agggaaggca tgaaggtcat ccggtaacag cattcctaga agatttatgg
    22441 ggattcttaa cagatatctt tggtaacttg tttggcagca acaatagctc tcagtctcag
    22501 taaatgacac atttgttgtc ttaatgtagt tattgtaact tattgaaaag cttgaggata
    22561 ctttgcttag ttcaagcctt tcccacacta atctgctata attgggataa tccctttagt
    22621 agtcaggcca cctctgatag gggtggcctt tttgggtttt agtgacccgt gtgttgagaa
    22681 aggagcattt atgccagaaa agaaagtgtt gcccgtccgt tgcctaatat gaaccgacag
    22741 ttattcctta ttaaattgac gcaaatatcc atgctttcac tgtggtggct aataaggact
    22801 aacataaaat ctcaaacgct ttcaaaaaca gctcctgagc ttaaaaaagg ctgatgaaca
    22861 gatttgttga acttaagaga gattgccatg attttctaat catttgatgt ttaaaatgat
    22921 aaaaacatga acttgaaatg aaagaatgcg agtagtaaac tataaccgta tcaaaaaata
    22981 atgaaatgga gtgattttat gaaaacaaaa tcaaaacgct ttttaaacct agcaaccctt
    23041 tgcttggccc tactaggaac gactttgcta atgggacgtc ccgtaaatgc ggaggtgaca
    23101 tatacaggtg agagatcaac atcagtgaat ggtcctggtg aagatagtag cagtgagaaa
    23161 acggaacaaa ctagtgatag tgacaaaaga gatcggaatc gtggttatga agagggttat
    23221 aagcgcggac aagagtcaga taatcgtgtt ctagatagag ggactattga ggttccctac
    23281 aatgtaatag acggagttga atataaggat ggctatgaac agggattcgc agcaggatgg
    23341 tacgatggtc atccaatcac agcactacta gacatagctt ggtcctactt aacatatgca
    23401 tttgatgttt tgtttggtgg tacaaattct tagtaaatgg cgtatttgtt gtcttaatgt
    23461 agttattgtg acttattgaa aagcttgagg atactttgct tagctcaagc ttttcccaca
    23521 cgaatctgtt ataattaaat tatttagttg ccacgctatc tttgataacg tggcttttcg
    23581 gtgtaggtga ggttatcagg atggatttat tttcggatct taataggaga caacatgaag
    23641 ttacagatcg taaagaagac gctagagtaa acagtgactc taattcagac aaatcaaaca
    23701 gatttagtat cttacgattt atttcttgtt tacgtaattt ctggagttgt caaatatacc
    23761 tgaagctgtg tattacagtg gttctacgac gacgcttgat cttgagcttt acgacttatc
    23821 cgtgaatagg tatattaacc aaattaattt taagtaataa ggagagatac tttaaatgac
    23881 aaaagcaata aagttggtta ttctcagttt gattctattt actacagttt ttctttcagt
    23941 tagacaagct agtgctgatc ttatgatgga tggagttact tattctgaaa gtacagatag
    24001 taatacattt ttatcaaact ctggagaggt agataacata tgggaaaagg atagagattt
    24061 tggaaaacaa cgaggacttg aggctggtaa aaatggactt ggcccaacaa taaatcgagg
    24121 tgatatacca gttcctgaaa aggtaaaaca taaagataaa tatcaggatg ggtatcaaga
    24181 aggttatatc caggcatggc atcaggagca tccatttcaa ggagttatat tcgctgtatt
    24241 cgatgttttt agtttgatat ggggtgtaat aagtgagctt ttcacttgag cgaattagtt
    24301 atagtgacgc tattagagag taatattatc tgttgaaagt actgtaatgt ttaggctaca
    24361 gcgctatttt tggtacatat agctaaaagt actacagtta attttttatc acaagccatt
    24421 cccacacaaa tctgctataa ttgggatagt ccctttagta gtcaggccac ctctgatagg
    24481 ggtggctttt ttgggtttta gtgacccgtg tgttgagaaa ggagcatgta tgccagaaaa
    24541 gaaagtgttg cccgtcctaa gcatggtggt gagccttgtg tctctgtttg atcatatctt
    24601 cttttttgct acttatagtt atctctactt gccgcttgta gcaagtagtc tagctcttat
    24661 cggtctggtg caaaacaaag gcaggcaaaa ggtttggtcg ctagctgggc ttatcctagc
    24721 tgctctagcc gtggcagtag tggcctatgc ctactatgac ccaaccgtca tcccctacta
    24781 accttccccc ctctttgcac gcaatcagca acacaaaaag cacccctgac aggtgctttt
    24841 tgtgctttag ccaacctcat agcctagtaa atcctcactg aaacagtccc ttaaagttcc
    24901 accactgatc ttggatgctt tcgcttaacc gctgatgcca aggtttatgg tcttcttctc
    24961 ttatctgctt tagcatgctt tcttctagct gtttttgttg cttttcctct tccttttttt
    25021 gaagtgcttc ttcagcttct ttttcttttt ttatagtatc agagtctcta tagatataac
    25081 taccgacata aaatccgtct tctcctttaa atgagaaagt taatttttct cctttcttca
    25141 aaggatcttc tcctaagtta agaatataag gtactcctgt ataatattga cgataaggat
    25201 ctgtttttgt catatttttt tcgagaatag gactagctaa aattcctcgt tctttactgt
    25261 tatgatcaca ttgtctgtaa agatataccc accatccttg agggatatat atttttacta
    25321 ctttactacc aacatataag tcttctagag tgattgtgtt gccgctataa tctttaattt
    25381 tttgttgagg ttcatccaaa tcaaatttta ctggcttttg tttactactt tgttcatcct
    25441 ccgctctgac gctactagtt accccgatgg caaagagtgc cagtagcaag gttaatgtct
    25501 ttttcatttt ttcctctcct tttcctcatt ttttaagaca agggttatta taccaattct
    25561 tgcgctaaaa agatagagag tttatgtctt gtggttggga aatggcgcga ctattcttgg
    25621 tagggtcaaa cagggtgact aaagttccta ttagcgcaac ggatatcgtc cataaaggag
    25681 aacaatatga catttgaaga gattctcgac cctagatagt tactgacttt catagggttt
    25741 cttatctagt agtattgagg attttatcac acaaatcgtt tcatagactt gttttaaaag
    25801 ccatcacaag tccactaaaa aggggataaa aaaatgcgtc cgcaggcaag aaaaaaggca
    25861 ccttacgggg tgtcttttaa ttaatttgtc aataataatt gacgtttatt ttgtaagtgg
    25921 tataatatag ttaataaaaa actcgtgaag gataaacgct gggtcccaaa atggggtagg
    25981 tacttagtac tgagcattcc tatgtgcctg gggttttttt atttgttcga ttttttaagg
    26041 ctctcaacaa agttttgagg gtcttttttt atttcattta ctataaattc aacaaactgt
    26101 tgtgaatatg tataagtctg tgttgatcct ataagatgac aatatgaaaa ttttgtttca
    26161 ctcttaatat tatagaaatc aatcactaaa tttaatacat aagtattgaa ccctttttca
    26221 tagccaagtt ttatcgcaga cttttttagc ctttcctgaa ctgctgttac tacattgcta
    26281 taggaatatt tatgagtatc tgacggatct tttaattctt taactagtga aacaggggtt
    26341 tcagcatctt ttgaaatact aacaacgaag tcagcctcat cttttttctt agtaatataa
    26401 agattttgtc taa
//
LOCUS       DAUDYX010000024        25298 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.024, whole genome shotgun sequence.
ACCESSION   DAUDYX010000024 DAUDYX010000000
VERSION     DAUDYX010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25298
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.024"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(<1..681)
                     /gene="speh"
                     /locus_tag="VHD02_000989"
     CDS             complement(<1..681)
                     /gene="speh"
                     /locus_tag="VHD02_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeH"
                     /protein_id="HEQ5497607.1"
                     /translation="MRYNCRYSHIDKKIYSMIICLSFLLYSNVVQANSYNTTNRHNLE
                     SLYKHDSNLIEADSIKNSPDIVTSHMLKYSVKDKNLSVFFEKDWISQEFKDKEVDIYA
                     LSAQEVCECPGKRYEAFGGITLTNSEKKEIKVPINVWDKSKQQPPMFITVNKPKVTAQ
                     EVDIKVRKLLIKKYDIYNNREQKYSKGTVTLDLNSGKDIVFDLYYFGNGDFNSMLKIY
                     SNNERIDST"
     gene            complement(707..1384)
                     /gene="spei"
                     /locus_tag="VHD02_000990"
     CDS             complement(707..1384)
                     /gene="spei"
                     /locus_tag="VHD02_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047373492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeI"
                     /protein_id="HEQ5497608.1"
                     /translation="MSSVGVINLRNLYSTYDPTEVKGKINEGPPFSGSLFYKNIPYGN
                     SSIELKVELNSVEKANFFSGKRVDIFTLEYSPPCNSNIKKNSYGGITLSDGNRIDKKN
                     IPVNIFIDGVQQKYSYTDISTVSTDKKEVTIQELDVKSRYYLQKHFNIYGFGDVKDFG
                     RSSRFQSGFEEGNIIFHLNSGERISYNLFDTGHGDRESMLKKYSDNKTAYSDQLHIDI
                     YLVKFNK"
     gene            complement(1658..2947)
                     /locus_tag="VHD02_000991"
     CDS             complement(1658..2947)
                     /locus_tag="VHD02_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysin"
                     /protein_id="HEQ5497609.1"
                     /translation="MHIDGSLGAQCWDGYADYCKYLGLPYANCTNTGYARDIWEQRHK
                     NGILNYFDEVETMQAGDVAIFMVVAGVTPYSHVAIFDSDAGSGYGWFLGQNQGGANGA
                     YNLVKIPYSTTYPTAFRPKVFKNAVTVIGNIGLNKGDYFIDVSAYQQADLTATCQQAG
                     TTKTIIKVSESLAWLSDRHQQQANTSDPIGYYHFGRFGGDSNLAQREADLFLSNLPTK
                     KVSYLVIDYEDSASADKEANTNAVIAFMDKIANAGYKPVYYSYKPFTLNNIDYQQIIA
                     KYPNSIWIAGYPDYEVRTEPLWEFFPSMDGVRWWQFTSVGVAGGLDKNIVLLADDSSK
                     VDIPKIDKPQSQLTFNQKLDTNTKLDNSNVPYYEATLRTDYYVESKPNASSADKEFIK
                     AGTRVRVYEKVNGWSRINASQSDQWVEDKYLSNATQV"
     gene            complement(3033..3218)
                     /locus_tag="VHD02_000992"
     CDS             complement(3033..3218)
                     /locus_tag="VHD02_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497610.1"
                     /translation="MNNWFKKVAIKTIKTMAQTAVGLIGSSVLITDINWPTMLSAVLL
                     SGLTCILMNVSQIKEEE"
     gene            complement(3215..3511)
                     /locus_tag="VHD02_000993"
     CDS             complement(3215..3511)
                     /locus_tag="VHD02_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497611.1"
                     /translation="MIDFVQIGAFCGAALSILGVWGFIVNPFKKAMEANEFAMAQLKD
                     SIKELAYELKNLDREREITKKIIDRHEERLGRVEDEVIINKERIITLFKKGEEK"
     gene            complement(3522..4133)
                     /locus_tag="VHD02_000994"
     CDS             complement(3522..4133)
                     /locus_tag="VHD02_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEQ5497612.1"
                     /translation="MRNWKVTGKYPQLDGTGAVASTHIIIAAEDGAVIPQLIKQDLTS
                     TNDTEIIKAALEEFKKSEYVEIAMGEAVQKVDDLEKISQETAKTAKTAQTAAGLAKVS
                     AERTQRMINLQTIHVLTSGGNVEPDIYKGMLELIEPAKQGEYQAYDVFTVVDESHEDQ
                     AVEGNLVFVHVNEPFTYEAQTLKDLESEDKVTVIKYADLVKQD"
     gene            complement(4136..4297)
                     /locus_tag="VHD02_000995"
     CDS             complement(4136..4297)
                     /locus_tag="VHD02_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497613.1"
                     /translation="MQEKLLGKIINDLALKVANLTLENAQLKAQHEIELEELNAQLDE
                     ATAPKEEGK"
     gene            complement(4311..6224)
                     /locus_tag="VHD02_000996"
     CDS             complement(4311..6224)
                     /locus_tag="VHD02_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp58-like family protein"
                     /protein_id="HEQ5497614.1"
                     /translation="MSRDPTLTLDESNLVIGKDGRVHYTFTASQDNQTVRLASNCLGT
                     AHFNQLMIERGDKATGYVAPVVVEGSGESTGVFKSLEEMLSGLQSINLELTDTKNSNL
                     WSKIKLTTNGMLREYHRDNITTEIIEKADGIATRISEDSDKKLALINDTIDGIRREYQ
                     DADRELSASYQAGIEGLKATMASDKLGLQAEIKMSARGLSQKYDDEIRKLSAKITTTS
                     SGTTEAYENKLEGLRAEFTRSNQGMRIELESQISGLRAVQQSTASQISQEIRDRTGAV
                     SRVQQDLDSYQRRLQDAEDNYSSLTHTVRGLQSDVVSPTGKIQSRLTQLAGQIEQRVT
                     RDGVMSIISGAGDSIKLAIQKAGGINAKMSGNEIISAINLNSYGVTIADKHIALDGNT
                     TVNGTFTTKIAEAIKIRADQIIAGTIDAARIRVINLNASSIVGLDANFIKAKIGYAIT
                     DLLEGKVIKARNGAMTIDLQSGQINHYTNESAMRRIDSSTASQFIKMTKSGFISEIGN
                     MQAAMTVIGSNSDGSENHENKTFGGIRIWNGKSSYQSTSFVELVGNRVAIYGNKNRSP
                     WLFDSTTSGYAYLIPQNDRGIKHVIGRADRKIDQIHVGDIYVQGERVAMMLKDLALKI
                     GYIGTGGWANRIG"
     gene            complement(6237..7358)
                     /locus_tag="VHD02_000997"
     CDS             complement(6237..7358)
                     /locus_tag="VHD02_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronoglucosaminidase"
                     /protein_id="HEQ5497615.1"
                     /translation="MTETIPLRVQFKRMKAAEWASSDVVLLEGEIGFETDTGFAKFGD
                     GQNTFSKLKYLTGPKGPKGDTGLQGKTGGTGPRGPAGKPGTTDYDQLQNKPDLGAFAQ
                     KEETNSKITKLESSKADKSAVYSKAESKIELDKKLSLTGGIVTGQLQFKPNKSGIKPS
                     SSVGGAINIDMSKSEGAAMVMYTNKDTTDGPLMILRSDKDTFDQSAQFVDYSGKTNAV
                     NIVMRQPSAPNFSSALNITSANEGGSAMQIRGVEKALGTLKITHENPNVEAKYDENAA
                     ALSIDIVKKQKGGKGTAAQGIYINSTSGTTGKLLRIRNLNDDKFYVKPDGGFYAKETS
                     QIDGNLKLKDPIANDHAATKAYVDGEVEKLKALLAAKQM"
     gene            complement(7355..9406)
                     /locus_tag="VHD02_000998"
     CDS             complement(7355..9406)
                     /locus_tag="VHD02_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ5497616.1"
                     /translation="MIYLFDKLERLVATVGTDDLLSWHFKVKNNDWDQASFEVPVDYD
                     IEPFVYFGFFNYDPNQKEDVFKLFKVIDYNLEDSKFYKGLDKAESDLDTIAIIKDKRF
                     RQSSADACIDGALEGTGYQVGKVEGITNVRTLSYYYISPRAALIKIVEAFNCEFNVRY
                     TFINNKITSRYIDLKKRFGKPTGKQFEHGNNLLKIVYEESTDDIVTCLIGRGKGEEIQ
                     HEEAEPKDVEGHLPQEERRQGYGRRIEFTDVVWSVEKGDPIDKPAGQNFVALDSAREE
                     YGLSQNGELKHRWGVFVNEEIEDKTELLKATWEELQRLSIPIRIYKAEILDIGPETWK
                     GDSVAIIYDEVKIAFETRVDEIDIDKLNFNRSVVTLGDYSVVQNRESRSRKEAVQNMI
                     DESLETITDLGMTFQEFLQGIEKRIETGKKEMEDNWRKVNLEFDNFKKKVEQEGLQFN
                     TLKEQIKEVDERTDKELEEFRATLKNLALPEEAIKKITETIKVDDIPSLKQNFDDLKN
                     KVSETSETARLNAEIIGTDGKTRYNKNLLVGDPNRTKTYDEDYIEVEANDGGFKRGET
                     YTISFSQTCELLKKVAVTLTQANNKGVKLVLTPTKAKMEAQTFEVAKDKQSIEVYPLS
                     YTAVLTGDWYKSKQIDLNASEVQELALEMAYKDVVDGNNATIAGQWSDSPQMILDGGK
                     K"
     gene            complement(9403..10182)
                     /locus_tag="VHD02_000999"
     CDS             complement(9403..10182)
                     /locus_tag="VHD02_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEQ5497617.1"
                     /translation="MYEFNDTIRGTPKVTFNLKTTIGGKVLEDELNNGFGTFRTLTVS
                     GRDVVDLEHQTTSVLGRNGEYFHNATVEVRKLEIKAKITGKDNQSMRLQYEKLNRLIV
                     SHNQVFLSFSDEPDRNYLGIFKSKVVPEEVSNEQIIGLTFICYNPFKMSDVKTKKGTS
                     IQNGGLFQTKPIITLNLSSPTKEIKLLHVESQKYIRLTGTYTTDEIKIDMATGKITQN
                     GRNILGNLDMVNSRYFELLPGKNTLQCDNATITAEFREVYL"
     gene            complement(10215..13850)
                     /locus_tag="VHD02_001000"
     CDS             complement(10215..13850)
                     /locus_tag="VHD02_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEQ5497618.1"
                     /translation="MGESYSVEAVLTAVDKTFGKTLQSAIRSIDGLEKRSTGFSSVSQ
                     KASSMFKSMLGANLAGQAISAMTRTVSSGLGSMLGEMNSSAKAWKTFDANLADIGFGK
                     KQILAAKTAMQDYATKTIYSASDMASTYAQLAAVGVKDTGKLVKAFGGLAASAENPKQ
                     AMKSISQQMTQAVGRPTVAWQDFRIMLEQAPAGMAKVAKSMGKNLDELVADIQAGRVK
                     TSDFLEAVKKAGNDKSFQKMATEFKTVDQAIDGMREGLSNKLQPAFEKVNQFGIRAIE
                     AIGKQLDKVDFSKFASNLGKFLEGINIDKIVSNISSAISSVTSKVKEFWGGFKQTGAI
                     SAFSGALKSVWGALKNVASAMSGGSWKNFGSIVGGIVKHVSNFAKAIADVVGKMEPGR
                     LQSWIATFAAVGGGLKLFEKLTGQSVVGSFLDKISTKFGLFGKKAKEGTDQAANGSRK
                     SGGIISQIFNGLGNIVKSAGTAISTAAKGIGTGIKTALSGAPPIISSLGTAISTVAQG
                     IGTGLAIAFRGLGAAIAMVPPTTWLALGTAILMVGAAFALAGTQADGISQILRTIGDV
                     VVQVLQQVTDSLATLLTIIANAIGSMLPIVAGAISQIVGAVAGGLSQLIIAVSTGVSL
                     VIGAFTGLLGGISGVINSISAVIQSLTGVITAVFNGIATVISSVGSTIKDVLTGLGTA
                     FEGFGNGVKSALEGVGAVIESFGSAVRNVLDGVANILDSMGTAALNAGRGVKEMAKGI
                     KMLVDLSLGDLVATLAAVASGLGKMASSAGEMTTLGSAMSKVANGMTRLATSATIAIT
                     GLTVFATTMATIKTAVATLPPVLTMAASGFTTFTTQAVAAVTGLAAINAPITMFKAQL
                     MTITPALAQAGAGFAAFVAQSSTFSTGLASAGPTIAAFNANLMSLSATTGVLVASIAG
                     LSAVLSVVSAGFSQIGASATATVGQIQAFASSTTVVSSAFASMQSMIQSAMAAIVSSI
                     ITSFNQAASQMQSILSRMLSQARTFGSQLEQQMRQSGQRSGQNLARGLSSQQGAVINA
                     ISSMVNAAVSRANAGAGPMRQAGAYIGQGLAQGMYSALGAVTAAANALVAQAERAARA
                     KAMIHSPSRLFAKRVGQYIPQGVAMGIDKNADVVDDSVGGLFDSINSFDFNIADRLTS
                     IGAKFQGVVKSESSQSLSQQQEFVHTAQPAYINFSLGGNEYEAFVSDITNQQAKIEKI
                     RLKRSSW"
     gene            complement(13865..14194)
                     /locus_tag="VHD02_001001"
     CDS             complement(13865..14194)
                     /locus_tag="VHD02_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497619.1"
                     /translation="MIVLNCIRYLGMTDINEIGRLTLYEYDLLMAGKALAAVDESHKA
                     HKQAWINHQVTATKLVGGKKNKKEVPVYKKFKDFFDYEEEIRKITQEIDEGYDKKGMD
                     LLLKANL"
     gene            complement(14236..14589)
                     /locus_tag="VHD02_001002"
     CDS             complement(14236..14589)
                     /locus_tag="VHD02_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HEQ5497620.1"
                     /translation="MQLEIKGKTHNVKFGTRFVAEMDKNHIAERQGFKFGAGLQSSVP
                     FLIDHSVVTLAEVIYTGTITEPPRPSLNDIYDYIDEVEDIEKLFDDVLDELRQSNASK
                     LFMAQVEKDLAEAKA"
     gene            complement(14649..15302)
                     /locus_tag="VHD02_001003"
     CDS             complement(14649..15302)
                     /locus_tag="VHD02_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEQ5497621.1"
                     /translation="MPESETLSPDIHTKSHEDSPNTVKKQEEVMETQLEAKQGIHSIL
                     LFRLLKEASSEAATKLAFQTEHEVGKSRDVDGQKTKDGIIQSVGALEYDFKATSILAK
                     GDVLAAKLEKAMENGELVEIWDIDLEETSKNGDSDNKLANVWGIDKNGTNRGNGKYLA
                     TYYQGYISSFSAKKNAEENIEIEMEFAINGVGQKGFATLTDAQKAAVQYAFKDTTKG"
     gene            complement(15312..15701)
                     /locus_tag="VHD02_001004"
     CDS             complement(15312..15701)
                     /locus_tag="VHD02_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEQ5497622.1"
                     /translation="MIKTRDQSIFDEMFKRIQSLGFTVYDYKPMTEVPYPFVEMESTD
                     AEYIPNKDDIKGSVELMLSVWGMQKKRKQVSDMASAIFSQALTVESSDVFRWSLNTRQ
                     SSIQMLDDTTTVTPLKRAIVTLRFNLR"
     gene            complement(15698..16063)
                     /locus_tag="VHD02_001005"
     CDS             complement(15698..16063)
                     /locus_tag="VHD02_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEQ5497623.1"
                     /translation="MTLLVADISLKVVGTAGLKKKLELIIKKDAVKKIVRDNGTQLQR
                     KMINKAVFTKGYSTGATRRSITMQIGDGGLSVKVKPGTHYAGYLERGTRLMSKQPFVL
                     PALKEQKVKFRKDLEALVK"
     gene            complement(16044..16352)
                     /locus_tag="VHD02_001006"
     CDS             complement(16044..16352)
                     /locus_tag="VHD02_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497624.1"
                     /translation="MRYADRVTFVKTTDEQYNPDLGEYTHTEVISITKPCFVMDMGME
                     KSVQIFGDYQKDRKVIYLKQPYTKAFDYCEYEGRRYKTQANKLGAIVFYLEGDDSIGG
                     "
     gene            complement(16349..16702)
                     /locus_tag="VHD02_001007"
     CDS             complement(16349..16702)
                     /locus_tag="VHD02_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEQ5497625.1"
                     /translation="MANVNTFSIVKNVKLDLGIEDDNQDQLLEMLLNRITDHFKANYG
                     VLEIDNAFSFVLEDCLIARFNRRGSERAKTEEVEGHKTTYYDHLNEFEPYDAMIMTKL
                     NLIKDKSRKGGLYFL"
     gene            complement(16714..16980)
                     /locus_tag="VHD02_001008"
     CDS             complement(16714..16980)
                     /locus_tag="VHD02_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497626.1"
                     /translation="MAKVIKTFRDKVTTNAYYVGDDYKGDRIEELTANGYLAGNTPKL
                     DTVEEVDLDKLKSDEIKAKLDELGIEHDSKLKKAELLELLKANI"
     gene            complement(16992..18041)
                     /locus_tag="VHD02_001009"
     CDS             complement(16992..18041)
                     /locus_tag="VHD02_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HEQ5497627.1"
                     /translation="MALIHEIITSENIKGFYNAKNENVENTLGEKAFPPKQQLGLKLS
                     FIKGAAGKPVTLKAAAFDTKVPLRDRMAVELLDEKMPFFKEAMSVKEADRQQLNILSQ
                     TKNNELIDTILASIYNDQATLIAGAKARLEAMRMEVLSKGKIHIQSNGVMKDIDYGLA
                     EDQTTKPDAKWDSAGTATPLKDIEKAIEKMAERGFVPEAIIMNSKTFSLIKNAESTLD
                     VVKPMAPNGAAVTKRDLNTYLEDELQIKVILKDGMFVGDDGESRKYFPDGFATLVPNG
                     NLGYTVFGTTPEQSDLLGGEATDANVSIVETGIAITTTKTTDPVNVQTKVSMIALPSF
                     ERLEEVHIINGLQDA"
     gene            complement(18044..18424)
                     /locus_tag="VHD02_001010"
     CDS             complement(18044..18424)
                     /locus_tag="VHD02_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HEQ5497628.1"
                     /translation="MNKRKVTTSKEILHNLDYEAISVTLDSNKIGKKVVPAGTILAGK
                     DKSIFEDRKQKVETVTNEEVSTKEYVDGILLTDVDLTNGDAVGSCVYRGTINADKLAD
                     SSVAENYDDLEEVLPHIVFIKGGK"
     gene            complement(18434..18967)
                     /locus_tag="VHD02_001011"
     CDS             complement(18434..18967)
                     /locus_tag="VHD02_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEQ5497629.1"
                     /translation="MAEETQVVETVEEQVVPEAKQPQDEKKYTDADVDAIIDKKFAKW
                     KSEQEAEKSEAKKMAKMNEKEKADYEKQKLLDELQELKNDKTRNELTAVARQMFAESE
                     INVNDDVLGLVVTLDAEQTKANVTTLANAFAKVIADDRKALVRQTTPSTGGGIAKQTN
                     YGANLASKAAQQSTKLF"
     gene            complement(19111..19377)
                     /locus_tag="VHD02_001012"
     CDS             complement(19111..19377)
                     /locus_tag="VHD02_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497630.1"
                     /translation="MNKRIKKKRKLETAVVMLVAENAMQAKALRNQNRRIAELRAIIQ
                     QNTQAINREFVTAKNTILDNQLAIKSIGDDVNHIKQNYKRKWRK"
     gene            complement(19763..19948)
                     /locus_tag="VHD02_001013"
     CDS             complement(19763..19948)
                     /locus_tag="VHD02_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497631.1"
                     /translation="MDFSDFLNKKQKEWDESHPIPDFSAMSDEELLYQPMSEALVSER
                     FAKELSKEVRKRNLLSK"
     gene            complement(19952..21514)
                     /locus_tag="VHD02_001014"
     CDS             complement(19952..21514)
                     /locus_tag="VHD02_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEQ5497632.1"
                     /translation="MTTRKSKYWRDRIKKEMDAKEADDISLEQSMKQLHDYHFRNIEK
                     EIESFYQRYADKEKIDLSEARKRASELDISAYQKKAKELVAKAEKLRKEGRTVTRDDF
                     THQENADMSIYNLAMKTNALELLRLNIDLEMQELANGEHKLTKKFLDEGYRKETEFQA
                     GLLGLSVASQASVKSLADAVINANFKGAKWSDNIWDRQDKLRSIISQSVQSAILRGKN
                     GLTIARDIRREFDVSASYAKRLAITEHARVQMEVGRLSMAENGFAMFDILPEPKACDV
                     CKDIAKHGPYHLDKWIIGENSPPFHPYCRCAVVGLDKKQEEKVNDKSEKFENITPNLM
                     PLFSKFASKITDLQRKIVYSADLADTGYIRTPHAFDINNTLRNKGYNYLNVDDKLITD
                     TLDSVISINSTPKNIKVYRFDDFELLGSINEQNNNIFDSGNFMDKLNQGGLSYTNDGY
                     TSASYDVKKNVMGYRPIKTEIKVPKGSHVYLTDNEEESEIILPRGTKYDIINAKINEY
                     EEIEITMEIRKE"
     gene            complement(21495..22997)
                     /locus_tag="VHD02_001015"
     CDS             complement(21495..22997)
                     /locus_tag="VHD02_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEQ5497633.1"
                     /translation="MYTESFRDSTGKTKTLEFRFHREARMRYQAESLESLLTEKYKLL
                     REMIEHHDKVQKPRIQELLDYAEGNNHTISEIGRRKDDDMADVRAVHNYGKYISTLKQ
                     GYLVGNPIRVEYIDGTEQQQDLLKDLSVKNNFHQLNRRLVKDLSKVGRAFELIYRSMD
                     DKTEVVRLDPREVFVIYQNNLEQSSLAGVRYYNKNQLDGTTKIVELYTDNKILKFEYD
                     GDLTPIGETSSHAFGSVPITEYLNTDDGMGDYETELSLIDLYDAAQSDTANYMQDLSD
                     AILAIIGRVSFPGYVDTAEKAIEYLRKMRKARLLNLEPPVDQDGREGSVDAKYLYKQY
                     DVQGTEAYKNRIVSDIHKFTNTPDMTDSKFAGQQSGEALKWKVFGLDQERVDMQALFE
                     QSLKRRYKLIARVSQLLKEIDDFDISKLKITFTPNLPKSLQEKIEAFKALGGELSQET
                     AMAITDIVEDAKKEISLINSESKSRSQLAQKLEETSRLTDRELAHDHQKE"
     gene            complement(23009..24298)
                     /locus_tag="VHD02_001016"
     CDS             complement(23009..24298)
                     /locus_tag="VHD02_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEQ5497634.1"
                     /translation="MKTTSLIKVDLPSTIGIGYGAFWRSRNFYRVVKGSRGSKKSKTT
                     ALNFIVRLLKYPWANLLVIRRYSNTNKQSTYTDFKWACNQLKVTHLFKFNESLPEITV
                     KATGQKILFRGLDDELKITSITVDVGALCWAWFEEAYQIETEDKFSTVVESIRGSLDA
                     PDFFKQITVTFNPWSERHWLKRVFFDEETKRADTFSGTTTFRVNEWLDDVDKRRYEDL
                     YKTNPRRARIVCDGEWGVAEGLVFDNFEVVDFDVEKTIQRVKETSAGMDFGFTQDPTT
                     LICVAVDLANKELWLYNEHYQKAMLTDHIVKMIRDKNLHRSYIAGDSAEKRLIAEIKS
                     KGVSGIVPSIKGKGSIMQGIQFMQGFKIYIHPSCEHTIEEFNTYTFKQDKEGNWLNEP
                     IDKNNHVIDAIRYALEKYHIRSNESNQFEVLRAGFGY"
     gene            complement(24276..24758)
                     /locus_tag="VHD02_001017"
     CDS             complement(24276..24758)
                     /locus_tag="VHD02_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEQ5497635.1"
                     /translation="MDWRWWKIAYEELTEKQQRFVDKYITTFNATESAKQAGYSEKSA
                     YSQGQRLLKNVEIQKAMKERFLEAKDTKGDRIQDVAETLEQDTSIARGEIQISEFKET
                     DMLTGQVVIHTKREYTPSHEEQGRARDRIYKVNGAYSEKRELEHSGTVVFANEDNIPD
                     "
     gene            24964..25176
                     /locus_tag="VHD02_001018"
     CDS             24964..25176
                     /locus_tag="VHD02_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497636.1"
                     /translation="MKDKKVATNRRSIIFISVPKSSIITTNLLEPEVGINEKLAKNIA
                     VTIAENDRDADIISVKFFKLFGEILK"
BASE COUNT         6779 a         5565 c         4343 g         8611 t
ORIGIN      
        1 agttgagtct attctctcgt tattggaata tatttttagc atgctattaa agtctccatt
       61 gccaaaataa tacaaatcaa aaacaatatc tttacctgaa tttaaatcta aggtaacagt
      121 tcctttagag tatttttgtt cccggttatt atagatatcg tatttcttaa tcaataactt
      181 tctaactttt atatccactt cctgagcggt tactttcggt ttattgactg taataaacat
      241 aggcggctgt tgtttacttt tatcccacac gtttatagga actttaattt cttttttttc
      301 tgaattagtt aatgtaattc caccaaacgc ttcatacctt ttccctggac attcacaaac
      361 ctcttgtgca gatagagcat aaatatctac ttctttatct ttgaattcct gtgatatcca
      421 atctttctca aaaaaaactg acaaattttt atccttgaca ctatatttca acatatggct
      481 tgttacaata tctggagaat tttttatact atcggcttca atcaagttag aatcatgctt
      541 ataaagcgat tctagattat gtctattggt tgtattataa gaatttgctt gaacaacatt
      601 ggaatataaa agaaatgaca aacatataat catgctgtag attttcttat caatatgtga
      661 gtagcgacaa ttatatctca ttttttctcc ttaagaagtg agagttttat ttattaaatt
      721 taactaagta tatatcaata tgaagttgat cagaataagc ggtcttatta tcactgtatt
      781 tttttagcat actctctctg tcaccatgtc ctgtatcaaa aagattataa gaaattctct
      841 ctccactatt caaatgaaaa ataatatttc cttcctcaaa gccagattga aatctagagg
      901 agcggccaaa atctttaaca tcaccgaatc cgtatatatt aaaatgtttt tgaagataat
      961 atcttgattt cacatcaagt tcctgaatcg taacctcttt cttatcagta ctcactgtac
     1021 ttatatctgt atagctatat ttttgttgaa cgccgtctat gaaaatatta acaggtatat
     1081 tttttttatc aattctatta ccgtcgctta aagtaatacc tccatatgaa ttctttttta
     1141 tattagagtt acagggagga gaatactcca aagtaaatat atccaccctt ttaccagaaa
     1201 aaaaattagc tttttctacg ctattaagtt ctacttttaa ttcaatcgaa ctattgccat
     1261 aaggaatatt tttgtagaac aaactacctg aaaatggcgg accttcattt atttttcctt
     1321 ttacttctgt tggatcatat gtagagtata aattccttaa attaataact cccacactac
     1381 tcatctcata cgcgtaacac gttggaatta tagctgatat aaatacccac aatagaaaag
     1441 atgaagttaa aaacttttta aaattaacat ttacaatttt tctcacttat aagtgtaccc
     1501 tattcttctt taataataag aaatcacaaa atattttagc acaaaaaaga aaaaaatcaa
     1561 cgaaaatgct taaataacaa ttaaaatctt tataacaaaa acagcccccg caaaagagag
     1621 cggttttgtc ttatctaaag gagctttacc tcctatttta tacttgtgtt gcattagata
     1681 aatacttatc ttcgacccac tggtcagact gagaagcgtt aatacgtgac catccattca
     1741 ctttttcata gactcttaca cgagttcctg ccttgataaa ttctttatca gcgctacttg
     1801 cgtttggctt agactctaca taatagtctg ttctaagggt tgcttcgtag taaggtacat
     1861 tcgagttgtc taatttagtg ttagtatcta gcttttgatt aaaagtaagc tggctttgtg
     1921 gtttgtcaat cttaggtata tccactttgc tactatcatc tgctaataat acaatatttt
     1981 tatctaaacc acctgctact cctacacttg tgaactgcca ccagcgcaca ccatccattg
     2041 aagggaagaa ctcccaaagt ggctctgttc gtacttcgta gtctggataa ccagctatcc
     2101 aaatgctgtt tgggtactta gcgataattt gctgataatc aatattatta agcgtaaatg
     2161 gtttatagct gtaataaaca ggcttatatc cagcgtttgc aattttatcc ataaacgcaa
     2221 taactgcatt agtgttagct tctttgtcgg cacttgcaga gtcttcataa tcaatgacta
     2281 agtaagagac ttttttggtt ggtaaattgg acagaaataa atctgcttct cgttgcgcta
     2341 agttgctatc tcctccaaat cgtccaaagt gataataacc aatagggtca ctagtattag
     2401 cttgttgctg atgcctatca gacagccaag cgagtgactc ggatactttg ataatcgttt
     2461 tagtagtgcc agcttgctga caagtagcgg ttaagtctgc ttgttgataa gctgatacat
     2521 caataaaata atcgccttta tttagtccta tattacctat aacagtaact gcatttttaa
     2581 aaacttttgg tctaaatgca gtaggataag tcgttgaata tggtattttt actaaattat
     2641 atgctccgtt tgctccaccc tggttttgac ctaaaaacca tccgtatcca ctacctgcat
     2701 cactatcaaa aatagccaca tggctatacg gtgtaacacc tgcaactacc ataaaaattg
     2761 cgacatctcc agcttgcata gtctctactt cgtcaaagta gtttaaaata ccatttttgt
     2821 gacgttgttc ccatatatcc cttgcatatc ctgtatttgt acagtttgcg tatggcagtc
     2881 ctagatactt acagtagtct gcgtagccat cccaacattg cgcaccaagc gatccatcaa
     2941 tatggataaa aaatcaaagc aaccacagat aatggcacat acagtattgc gattgctata
     3001 actaatgcta atcgtgtgat tgctcgcatg acctattcct cttccttgat ttgtgacaca
     3061 ttcatcagga tacatgttag tcctgacaac agcactgctg acaacattgt tggccaatta
     3121 atatccgtga ttaacacgct tgacccgatt agaccaacgg ccgtttgtgc cattgtttta
     3181 attgttttaa ttgctacttt tttaaaccag ttattcattt ttcttctcct tttttaaata
     3241 gcgtaataat acgctccttg ttgataataa cttcgtcttc tacgcgacct aaacgctcct
     3301 cgtgtctgtc gataattttt ttggtaatct cacgttcacg atcaagattt ttaagctcat
     3361 aagctaactc cttaattgag tccttgagtt gagccatggc aaactcatta gcttccatgg
     3421 cttttttaaa gggattgacg ataaatcccc aaacacccaa gatagacaaa gcagcgccac
     3481 aaaaagcacc aatttgtaca aaatctatca tctaaccacc tctaatcttg tttaaccaaa
     3541 tccgcatatt taataactgt gactttatcc tctgattcca aatctttaag agtttgcgcc
     3601 tcataagtaa acggctcgtt gacatgtaca aagacaaggt tcccttctac cgcttgatct
     3661 tcgtgcgact catctacaac agtaaacacg tcataagcct gatactcgcc ttgtttagca
     3721 ggctcaataa gctctaacat acctttgtag atatcgggtt caacgttccc accacttgtc
     3781 aatacgtgga tggtttgcaa gttaatcatt cgctgtgtac gctctgcgga caccttagct
     3841 aatcctgcgg ctgtttgggc tgttttagcg gtcttagcgg tttcctgtga gattttttca
     3901 aggtcgtcta ctttttgcac ggcttcgccc attgcaattt caacgtattc agatttttta
     3961 aattcttcca aagcggcttt gataatctct gtgtcattag ttgaggttaa gtcttgctta
     4021 ataagttgcg ggataacagc tccatcctca gcagcgataa taatatgtgt gcttgcgact
     4081 gctcctgtgc catcaagttg tggatatttt cctgttactt tccaatttct catgtttatt
     4141 taccttcttc cttcggtgct gttgcttcat ccaattgtgc gtttagttct tccaattcga
     4201 tttcatgctg tgcttttaat tgagcatttt ctagcgttaa attagcaact ttaagtgcta
     4261 aatcgttaat aattttacct aataattttt cttgcattgt ttctcctaat ttatcctatg
     4321 cggttagccc aaccacctgt acctatatag ccaattttaa gtgctaaatc ttttaacatc
     4381 atggctacac gttcgccttg cacatagata tctccaacgt ggatttggtc tatcttacga
     4441 tcagctctcc ctatgacatg ctttatacct ctgtcatttt gagggataag gtaagcatat
     4501 cctgacgttg ttgagtcaaa gagccatgga ctacgatttt tatttccata gattgctaca
     4561 cggttaccaa ctaattcaac aaaacttgtc gattgataag atgacttccc gttccaaatt
     4621 cttatgcctc cgaaggtttt attttcatgg ttttctgacc catcgctatt agagcctata
     4681 accgtcattg cagcttgcat gttacctatc tcagatataa agcctgactt tgtcatcttt
     4741 ataaactgac tagctgtact gctatcgata cgtctcatgg ctgactcatt tgtgtaatgg
     4801 ttgatttgac cgctttgcaa gtctattgtc atcgcaccat tacgagcctt aataacttta
     4861 ccctcaagca aatcagtgat agcatagcca attttagctt tgataaagtt agcgtctaaa
     4921 ccaacgatac tacttgcgtt aaggttaatc actctaatcc tagcagcgtc aatcgtgcct
     4981 gcaataatct gatcagccct aatcttgata gcctctgcta tctttgtggt aaaggtaccg
     5041 ttgacagtcg tattgccatc gagagcgatg tgtttatctg cgattgttac tccgtaggag
     5101 ttgaggttaa ttgctgagat aatctcatta ccagacattt tggcattaat gccgccagcc
     5161 ttttggatag ctaatttaat gctgtctcca gcgccactaa taatactcat gacaccatct
     5221 ctagtaaccc gctgctcaat ttgtcctgct agttgagtaa ggcgtgattg gattttacca
     5281 gtcggggata ccacatcact ctgcaaacct ctaactgtat gggttaagct actgtaatta
     5341 tcttccgcat cctgcaaacg acgctgataa ctgtctaagt cttgttgcac acgactgact
     5401 gctcctgtcc tgtctctaat ctcttgtgag atttggctag ctgttgactg ctgtacagct
     5461 cttagtccgc tgatttgaga ctcgagctct atcctcatgc cttgattact acgagtaaac
     5521 tcagcacgta aaccctcgag cttgttttcg taggcctcgg tcgtgccgct tgaggttgtt
     5581 gtgatcttag ccgatagctt acgtatctcg tcatcatact tttgagataa gcctcgggct
     5641 gacatcttaa tctcagcttg caagccaagt ttatcgctag ccattgtggc ttttagtccc
     5701 tcgatgcctg cctgatagct tgcggatagc tccctatcag catcttgata ctcacgtctg
     5761 ataccatcaa tggtgtcatt gatgagcgct agctttttgt cagaatcctc gctaatccgt
     5821 gtggcaatac catcagcttt ttcgatgatt tccgttgtga tgttatcacg atgatactca
     5881 cgtaacatgc cgtttgtcgt cagcttgatt tttgaccaaa gattggagtt tttagtatct
     5941 gtcagctcta aattaataga ctgtagacca ctaagcatct cctcaagact tttaaacaca
     6001 cctgtcgact caccgctacc ctcaacaacc acaggcgcca catagccagt cgctttatcc
     6061 cctcgctcaa tcatgagctg attaaagtgc gctgtgccta agcagttgct ggctagtcta
     6121 actgtttggt tgtcttgact agcagtaaat gtataatgca cacgtccatc cttaccaata
     6181 acgagatttg actcgtctaa tgtcaacgtt ggatctctac tcatttatcc tcctttttac
     6241 atttgcttag ccgctaagag tgcttttaat ttttcgactt caccatcaac ataagctttg
     6301 gttgccgcat gatcattcgc tatgggatcc ttgagtttca ggttgccatc aatctgcgaa
     6361 gtttccttgg cataaaaacc accgtcaggc ttgacgtaga acttatcatc attaaggttt
     6421 ctaatcctaa gcaacttccc tgtcgtgcct gatgttgagt taatgtagat tccttgggca
     6481 gcagtacctt ttccgccttt ctgtttttta acgatatcaa tagataacgc tgcagcgttt
     6541 tcatcgtatt ttgcctcaac gtttgggttt tcgtgtgtga ttttgagcgt tcctagcgct
     6601 ttttcgacgc ctctaatttg catcgcacta ccgccttcgt tggcactggt tatattaagt
     6661 gccgaggaaa aattaggtgc gcttggctgg cgcattacaa tatttacagc attagtctta
     6721 ccgctgtaat ccacaaattg agctgactga tcaaacgtat ctttgtcaga acgtaaaatc
     6781 atcaatggtc catcagtagt atctttattt gtatacatca ccatagcagc accttccgat
     6841 ttagacatat caatgttaat cgctcctcct acggaagatg agggtttaat accactttta
     6901 ttaggtttaa actgtagttg tcctgtcact atgccgcctg ttaagctcaa ttttttgtct
     6961 agctctattt ttgactctgc ttttgagtaa acagcgcttt tatctgcttt gcttgattct
     7021 aatttggtga ttttactatt agtttcttct ttttgtgcaa acgcacctag atctggttta
     7081 ttttggagtt gatcataatc tgtcgttcca ggcttgccag cagggccccg aggaccagtt
     7141 cctccagttt taccttggag accagtgtct cctttaggac ctttgggacc agtaaggtac
     7201 ttaagcttac taaaagtgtt ttgaccatcg ccaaacttag caaaaccagt gtcagtctca
     7261 aagccaatct caccctccag taagacgaca tcactacttg cccactcggc ggctttcatc
     7321 cgcttaaatt ggactcttaa tggtatagtt tctgtcattt tttacctcca tctaaaatca
     7381 tttgcgggct gtctgaccac tgccctgcta tcgtggcatt attaccgtca accacatctt
     7441 tataagccat ctcaagggcc aattcctgca cctccgacgc gtttaaatct atctgcttag
     7501 atttatacca gtcgccagtt aaaacagccg tgtagctcaa aggataaacc tctatagact
     7561 gtttatcctt agcgacctca aacgtctgtg cttccatctt agccttggta ggtgtcagca
     7621 ctaacttaac tcccttgttg ttagcctgtg tcagcgtgac agccactttt ttgagtagct
     7681 cacaagtctg gctaaagcta atcgtgtagg tctcgccacg cttaaaacca ccgtcgttag
     7741 cttctacttc tatgtaatcc tcatcatagg ttttggtgcg gttagggtcg ccaaccagca
     7801 aatttttgtt gtagcgggtc ttaccgtctg taccgataat ctcggcattt agacgtgccg
     7861 tctcgcttgt ctcgctgacc ttgtttttta agtcatcaaa gttttgctta agagacggga
     7921 tgtcatcaac tttgatagtc tctgtgattt ttttaatcgc ttcctctggt aacgctaggt
     7981 ttttgagggt ggctctaaat tcttcaagct ctttatcggt gcgttcatca acttctttga
     8041 tttgttcttt caaggtgttg aattgcaagc cttcttgctc aaccttcttt ttaaagttat
     8101 caaattcaag gttaactttg cgccaattgt cttccatctc ctttttgcca gtctcgatgc
     8161 gtttttcgat gccttgcaaa aattcctgaa atgtcatccc taagtctgtg atagtctcta
     8221 aagattcatc tatcatgttt tggacagcct ctttacgaga ccgtgactca cgattttgga
     8281 caacgctgta atcaccaagt gttacgactg acctgttaaa attaagcttg tcaatatcaa
     8341 tctcatcaac ccgagtttca aaagctattt ttacctcatc atagataatg gctactgagt
     8401 cgcctttcca cgtctctgga ccaatgtcta agatttctgc cttgtaaatt ctgataggga
     8461 ttgacaaacg ctgcaattct tcccaagttg cttttaagag ttctgtctta tcctcgattt
     8521 cctcattgac aaagacaccc cagcggtgtt ttagctcacc attttgagac aagccgtatt
     8581 cttcccttgc gctatctagt gctacaaagt tttgaccagc tggtttgtct atcgggtcgc
     8641 ctttttcgac cgaccagaca acatcagtaa attcgattct tcgaccgtag ccttgccgcc
     8701 tttcttcctg tggcaagtgt ccctcgacat ctttaggctc agcttcttcg tgttggattt
     8761 cctcaccctt acctctgcca atcagacaag tcacaatgtc atctgttgat tcctcgtaga
     8821 caatttttag caggttgttg ccatgttcaa attgcttgcc tgttggcttt ccaaagcgct
     8881 ttttgaggtc gatataacga ctagtgattt tgttattaat aaaggtatac ctgacattga
     8941 actcgcagtt aaaagcttcc acaatcttaa tcagagccgc tcgtggactg atgtagtaat
     9001 aactaagtgt tctaacattt gttattccct caactttgcc gacttgataa cccgtaccct
     9061 ctaaagcacc atcaatacaa gcatctgcgg acgattgtct aaagcgctta tctttgataa
     9121 tggcaatggt atcaagatca ctctcagctt tgtctaagcc tttgtaaaac ttgctatctt
     9181 ctaggttgta atcaatgact ttaaagagct taaagacatc ttctttttga ttagggtcgt
     9241 agttaaaaaa accaaagtag acaaatggct ctatatcata atccaccggt acctcaaaac
     9301 tagcttgatc ccagtcgtta tttttgacct taaaatgcca cgagagcaaa tcgtcagtac
     9361 caacagtggc gaccaatcgc tccagtttat caaagagata aatcatagat aaacctccct
     9421 aaactctgca gttatggtgg cattgtcaca ctgcaaagta tttttaccag gtagtagctc
     9481 aaaatagcga ctgttaacca tatctaaatt gccaaggata ttgcgtccgt tctgggtaat
     9541 cttacccgtg gccatatcaa tcttgatttc gtcagtggta taagttccag tcaatctgat
     9601 atacttctgg ctttcgacat gcaacaactt aatttctttt gttggtgatg ataggttgag
     9661 agtgatgata ggctttgttt gaaataaccc accattttgg atagatgttc cttttttagt
     9721 ttttacatca gacattttaa acggattgta gcagatgaat gttagtccta taatctgctc
     9781 gttagaaact tcttctggaa caactttaga tttaaagata cctagataat ttctgtcagg
     9841 ttcgtctgaa aatgataaaa aaacttgatt gtgactaaca attaatctgt ttaatttttc
     9901 atattgcaaa cgcattgact ggttatcttt tccagtgatt ttagctttta tttctagttt
     9961 tctgacttcg actgtggcat tgtggaaata ctcaccattt cgccctaaaa cacttgttgt
    10021 ttgatgctct aagtccacca catcacgacc agaaacagtc aatgtcctaa aggtaccgaa
    10081 accattattg agttcgtctt ctaatacctt tccaccaatt gttgtcttta aattaaaagt
    10141 aactttcgga gtacctctga tagtatcgtt aaattcgtac attttactcc tttcaaaaaa
    10201 actaagagaa acaactacca gctgcttctc tttagtctga ttttttcaat ttttgcttgt
    10261 tgattagtga tgtcacttac aaatgcttcg tattcgtttc cgcctaaact aaagtttata
    10321 tacgctggtt gagctgtatg tacaaactct tgttgctgcg ataacgattg cgaactctct
    10381 gatttgacaa caccttggaa tttagctcca atgctagtca gtctatctgc gatattaaaa
    10441 tcaaagctat tgatgctatc aaataacccg ccaacagagt cgtcaacgac atcagcgttt
    10501 ttgtcgatac ccatagctac cccttgcggg atatattgac caactcgttt tgcaaacaac
    10561 cttgacggcg aatgaatcat cgccttggct cttgctgctc tctcggcttg tgctacaagg
    10621 gcgtttgctg cagctgttac agctcctagc gctgaataca ttccttgcgc aagcccttgt
    10681 ccgatgtacg ctccagcttg acgcatagga ccagctcccg cgttggctct tgataccgca
    10741 gcattaacca tgctagaaat agcattaata acagcacctt gttgagaaga tagcccccga
    10801 gcaagatttt gtcctgaacg ctgtcccgat tgtctcattt gttgctctag ttgagaccca
    10861 aatgtcctgg cctgagatag cattcgagat aagattgatt gcatttgaga ggccgcttga
    10921 ttaaatgatg ttataatgct gcttactatt gcagccatgg cagattgaat catagattgc
    10981 atgctagcaa atgctgacga aacaactgtt gtactagaag caaaagcttg tatttgacca
    11041 acagttgctg tcgcagaagc ccctatttgg ctaaagccag ctgatacaac agaaagcaca
    11101 gctgataaac cagctattga tgcaactagc actcctgttg ttgcagataa gctcatcaaa
    11161 ttagcattga atgctgctat tgtaggaccg gcagatgcta aacctgtact aaatgttgat
    11221 gattgagcaa caaacgcggc aaagccagcg ccagcttgtg ctagagctgg tgttattgtc
    11281 attagttgag ctttaaacat agtgattgga gcattaattg cagccaatcc agtcactgct
    11341 gccaccgcct gagtagtaaa tgtggtaaac ccactcgctg ccatcgttag gactggcggt
    11401 agagttgcaa ctgctgtctt aatagttgcc atggtggtgg caaagactgt taatccagta
    11461 attgctatcg tagcacttgt tgctagacgt gtcataccat tggctacttt gctcatagca
    11521 gaacctaatg ttgtcatttc gccagctgag ctagccatct tccctagacc gcttgccaca
    11581 gctgctaatg tagcaaccaa atctccaagg gataaatcaa caagcatctt aataccttta
    11641 gccatctctt tgacgccacg gcctgcatta agtgccgcag tccccataga atcaaggata
    11701 tttgcaacac cgtcaaggac attcctaact gcactaccaa atgattcaat tactgcccca
    11761 acaccttcta gagctgattt tacaccattc ccaaatcctt caaaagcggt tcctagaccc
    11821 gtcaatacat ctttgatagt cgaaccgaca gatgaaataa cagtagctat gccattgaat
    11881 actgcggtaa tcacaccagt tagcgattgg ataacagcgc taatggagtt aataacccca
    11941 gaaataccac caagaagtcc tgtgaaagct cctataacga gagatacccc tgttgaaacg
    12001 gctataatga gctgagataa tccgcccgct actgcgccta caatctgaga gatagctcca
    12061 gctacaattg gcaacataga gccaatagcg tttgcgataa tagttagtaa agtggctaga
    12121 ctatcagtga cctgttgtaa aacttgtaca acaacatcgc caatagtcct taaaatttga
    12181 ctaatgccat cagcctgagt tcctgctaag gcaaaagccg ctcctaccat taaaatagcc
    12241 gttcctaaag ctagccaagt ggtgggaggc accatagcga ttgcagctcc taaacctcta
    12301 aaagcgattg ctagcccagt gcctatacct tgcgcaactg ttgatattgc ggttcctaga
    12361 gaactaatga taggaggtgc cccagacaag gcggttttaa tccctgtacc gatacctttt
    12421 gcagctgttg atatggctgt accagcagac ttaacgatat tacccaagcc attaaagatt
    12481 tggctgatga ttccaccact tttacgagag ccattcgctg cttgatcggt tccttcttta
    12541 gcttttttgc caaataatcc aaattttgta ctgattttat ctaaaaaaga gccaacaacg
    12601 ctttgtcctg ttagcttttc aaataacttt aaccctcccc cgactgctgc aaaagtggct
    12661 atccagcttt gcaatctgcc aggttccatt ttaccgacaa catcagcaat agcttttgca
    12721 aaattagaca catgctttac aattccgcct acaatagagc caaagttttt ccaactgcct
    12781 ccactcatag cgctagctac attttttaac gctccccaaa cactttttaa agctcctgaa
    12841 aaagcactaa ttgctccagt ttgtttgaaa ccgccccaaa attctttaac ctttgaagtg
    12901 acagatgaaa tcgccgatga aatattagat acaattttat cgatattaat tccttcaagg
    12961 aatttcccaa gattactagc aaacttagaa aaatcaactt tatcgagttg tttaccgatt
    13021 gcttcgatcg ctctaattcc aaattggttc actttttcaa acgctggttg caatttgttg
    13081 gataagcctt ctcgcatacc gtcgatggct tggtcaacag ttttgaactc agttgccatc
    13141 ttttggaaac tcttatcatt gcctgctttt tttaccgctt ccaaaaaatc gctggtttta
    13201 accctacccg cctggatatc ggcgacgagt tcatcaagat ttttacccat agatttagcg
    13261 actttagcca tccctgcagg cgcctgttcc agcattatcc taaagtcttg ccatgcaact
    13321 gttggtcttc ctactgcttg cgtcatttgt tgactgatag acttcatggc ctgcttcggg
    13381 ttttcagcag atgcagctaa accgccaaaa gcttttacga gctttccggt atctttcaca
    13441 ccaactgccg ctaactgtgc atacgtgcta gccatatctg atgccgagta gattgtttta
    13501 gttgcatagt cttgcatcgc cgttttagct gccaaaattt gtttttttcc aaacccaatg
    13561 tccgctaaat tggcgtcaaa agttttccac gctttcgctg aactattcat ctcgccaagc
    13621 atagagccaa ggcctgatga cactgtcctt gtcattgctg agatagcttg tccggctaaa
    13681 ttcgctccta acatggattt aaacatggaa ctagcttttt gagacaccga tgaaaaaccg
    13741 gttgaacgct tttccaagcc atcgattgaa cggattgccg attgtaatgt tttgccaaag
    13801 gttttatcaa cagctgtcaa aaccgcttca acagaataag attctcccat ttaacctcct
    13861 ttctttaaag gttagctttg agaagtaaat ccataccttt cttgtcgtaa ccttcatcaa
    13921 tttcttgagt gatttttcgg atttcttcct cataatcaaa aaagtccttg aattttttat
    13981 aaacagggac ttctttttta tttttcttgc caccaacaag ttttgttgcc gtaacttggt
    14041 ggtttatcca agcttgtttg tgagctttat gtgattcatc aacagccgct agtgctttgc
    14101 ctgccattaa taaatcatat tcatacaaag ttaatcgccc gatttcattg atgtcagtca
    14161 tgccaaggta tctaatgcaa ttaagcacaa tcatctcaaa gctttcttga gaagaataac
    14221 tcttttttat atttgttacg ctttggcttc cgccaggtct ttttccacct gagccataaa
    14281 caactttgat gcgtttgatt gacgtagttc gtctaaaaca tcatcaaaaa gcttttcaat
    14341 atcttcaact tcgtcaatat agtcgtaaat atcattcaaa ctaggacgtg gtggttcggt
    14401 gattgtacct gtataaatta cttccgcaag agtaacaacg ctgtggtcga ttaaaaacgg
    14461 aacgctagat tgtagaccag ctccaaattt aaatccctga cgttctgcta tatgattttt
    14521 gtccatctcg gcaacaaatc gtgtaccaaa ttttacgtta tgagttttac ctttaatttc
    14581 caattgcatt attctttctc ctttaaaaac ttaaaaataa aagaccagta attaactggc
    14641 cttgttgatt aacctttcgt tgtatcttta aatgcgtact gtacagctgc tttttgggca
    14701 tctgttaatg tagcgaatcc tttttggcca acaccgttga tggcaaattc catttcaatt
    14761 tcaatatttt cctcagcatt tttcttagca ctgaagcttg agatgtaccc ttggtaataa
    14821 gtcgctaggt atttaccgtt tcctctgttt gttccatttt tatcaatacc ccaaacgttt
    14881 gcaagcttat tgtcgctgtc gccgtttttg cttgtttctt ctaaatcaat atcccaaatt
    14941 tctacaagct caccattctc catggctttt tctagtttag ctgctagtac gtcaccttta
    15001 gctaaaatag atgttgcttt aaagtcgtac tccaaagccc ccacggactg gataatacca
    15061 tctttagttt tttgtccatc tacgtcacgg cttttaccta cttcatgctc tgtttgaaaa
    15121 gcaagtttag ttgctgcctc actagatgct tctttcaaca aacgaaataa caagattgaa
    15181 tgaatccctt gttttgcttc taattgtgtt tccattactt cctcctgttt ttttacagta
    15241 tttggactat cttcgtgact tttggtatga atgtcagggg ataatgtttc tgattctggc
    15301 atttctacct cctatctcaa attaaatcta agtgtgacaa tcgctcgttt aagaggtgtc
    15361 acagttgttg tgtcatctaa catttgaata gatgattgac gagtatttaa tgaccagcgg
    15421 aaaacgtcag aactctctac tgtcaaagct tgcgaaaaaa tagcagatgc catgtcagac
    15481 acctgctttc gttttttctg catcccccaa acagacaaca taagttcaac agaaccttta
    15541 atgtcgtctt tatttggaat atactccgca tcagtagatt ccatttccac aaatggatat
    15601 ggaacttcag tcatcggttt ataatcgtaa actgtaaagc ctaaagactg gatgcgttta
    15661 aacatttcat caaaaataga ctgatctcta gttttaatca tttgacaagc gcctccaaat
    15721 cttttctaaa ttttactttt tgctctttta gagctggcaa aacaaacggt tgtttgctca
    15781 taaggcgagt tcctctttca aggtagccgg cataatgagt tcctggttta accttgacac
    15841 tcaatccacc atcgccgatt tgcatggtaa tagaacgtct agttgcacct gttgaatagc
    15901 cttttgtaaa taccgcttta ttaatcattt tcctttgaag ctgcgtccca ttgtctctga
    15961 caatcttctt gacggcatct tttttgataa taagctcaag tttctttttt aaaccagctg
    16021 ttccaactac ttttaaagat atatcagcca ccaatagagt catctccttc cagataaaaa
    16081 acaatagcgc caagcttatt tgcctgcgtt ttgtatctcc tgccctcata ctcgcaataa
    16141 tcaaatgctt ttgtataagg ctgctttagg tagataactt tacgatcctt ttgataatct
    16201 ccaaaaatct gtacggactt ttccatgccc atgtccatca caaaacaagg ttttgtgata
    16261 cttatgacct ctgtgtgcgt atactcacct aaatcgggat tgtattgctc atccgtcgtt
    16321 ttaacaaatg taactctatc tgcatatctc ataaaaagta cagtccccct ttacgagatt
    16381 tgtctttgat taaattaagc tttgtcataa tcatggcatc gtagggttca aactcattca
    16441 aatggtcgta gtaggttgtt ttatgacctt ccacttcctc tgttttggca cgctctgaac
    16501 ctctacggtt aaaacgagca atcagacaat cttccaagac aaaagaaaaa gcattgtcaa
    16561 tttctaacac gccataattt gctttgaagt ggtctgtaat acgatttagc aacatctcca
    16621 acaactggtc ttgattgtcg tcctcaatgc ctaaatcgag cttgacattt tttacaatgc
    16681 taaatgtgtt tacatttgcc ataaacacct ccgttaaatg ttagctttta aaagctccaa
    16741 caattccgcc tttttaagct tagaatcgtg ctcaatgccc aattcgtcaa gttttgcctt
    16801 gatttcatcg cttttgagtt tatctaggtc aacttcttcg acagtatcca gtttaggagt
    16861 atttccggcc aagtaaccat tggctgtcaa ttcctcgata cgatctcctt tgtaatcatc
    16921 gcctacataa taagcattag tcgtcacttt atctctaaaa gtcttaataa ctttggccat
    16981 aaagcacctc cttatgcatc ttgcaagcca ttaataatat gtacttcttc caagcgttca
    17041 aatgatggca aggcaatcat agataccttg gtttggacgt ttactggatc agttgtcttg
    17101 gttgttgtga ttgcgatacc agtttcgaca attgagacat ttgcatctgt cgcttcacca
    17161 ccaagcaagt ctgactgttc tggagttgtg ccaaatacag tgtagccaag gttgccgtta
    17221 ggtactaatg ttgcaaaacc atctgggaag tatttacgag attcaccatc atcaccaaca
    17281 aacatgccat cttttaggat gactttgatt tgtagttcat cttcaagata tgtgtttagg
    17341 tcacgtttag taaccgctgc cccatttggt gccataggct tcacgacatc taaggtgctc
    17401 tctgcgtttt taatcaaact aaatgtctta gagttcatga tgatagcttc tggcacaaaa
    17461 ccacgctctg ccattttttc gatagccttt tcaatgtctt taagtggtgt agctgttcct
    17521 gcgctatccc atttggcgtc tggtttggtc gtttgatctt cggccaatcc atagtcgatg
    17581 tctttcatca cgccgttaga ctggatatgg attttacctt ttgatagtac ttccatgcgc
    17641 attgcttcaa ggcgtgcttt agcacctgca ataagcgtag cctggtcatt gtagatagat
    17701 gccaagatgg tgtcgataag ttcgttattt ttagtttgag acaaaatgtt aagttgttga
    17761 cgatctgcct ctttaacaga catagcttcc ttgaaaaatg gcattttttc gtcaagcaat
    17821 tcaacagcca tgcggtcacg tagaggcacc ttagtgtcaa aagcggccgc tttaagagta
    17881 accggttttc ctgctgcacc tttaataaat gacaacttaa ggcccagttg ttgttttggc
    17941 gggaatgctt tttctcccaa tgtattttca acattttcgt ttttagcgtt ataaaaacct
    18001 ttgatatttt ccgatgtgat aatttcgtgg atcaatgcca tgtttattta cctcctttga
    18061 taaagacaat gtgtggtagt acttcttcca aatcatcata attttccgca acagatgaat
    18121 cagcaagctt gtctgcattg atagtcccac gataaacaca ggaaccgact gcatcgccat
    18181 ttgttaaatc gacatctgta agtaaaatac catcgacata ttcttttgta gagacttctt
    18241 catttgtcac ggtttctact ttctgtttgc ggtcttcaaa aatggattta tctttgcctg
    18301 ccaaaattgt tccagctgga acaactttct tgccgatttt gtttgaatcc aaagttactg
    18361 aaatagcttc atagtcgaga ttgtgtagaa tctctttaga tgttgttact ttacgtttgt
    18421 tcataatttc ctcctaaaaa agcttggtgc tttgttgtgc tgccttacta gccaagttag
    18481 caccgtaatt ggtttgtttt gcgataccac cgcctgttga cggagtggtc tggcgtacaa
    18541 gagccttgcg gtcatcagcg ataactttag caaatgcgtt tgctagcgtt gttacatttg
    18601 cttttgtttg ttctgcgtcc aaagtcacaa ctaaaccaag tacgtcatcg ttgacgttga
    18661 tttcagattc tgcaaacatt tgacgagcta ctgctgttaa ctcattgcgt gtcttatcgt
    18721 tttttagctc ttgcaattcg tctaacagct tctgcttttc atagtctgct ttttctttct
    18781 cgttcatctt agccattttt ttggcttccg atttctcagc ttcttgttct gacttccact
    18841 tcgcaaactt tttgtcgata atagcgtcca catctgcatc tgtatacttt ttttcgtctt
    18901 gcggttgttt tgcttcaggt accacttgct cttcaaccgt ctcaactact tgtgtttctt
    18961 ctgccatgat tggcacctcc tatgttttaa atcgtccccg attattaatt ccatagcttt
    19021 tatcgtcgtc aatgcttgga cataataaaa aaaccgtatt cctacgactt agtttgattc
    19081 taaaggggtc gaattcgacc agtttaaaat ttattttcgc cacttccgct tataattttg
    19141 cttgatgtga ttaacatcat caccaattga cttaatagct aattggttat ctaaaatcgt
    19201 atttttagca gttacaaact ctctatttat tgcttgggtg ttttgttgta taatcgctct
    19261 cagctctgca attcgtctgt tttgatttct aagagctttt gcttgcatag cattttctgc
    19321 aacaagcatc acaactgctg tttctaactt acgttttttc ttgatgcgtt tattcatatt
    19381 tattcccctt tgtacaacca gctaaaatca ttatcctgca atacttgata caaaatcaga
    19441 ccaatgcggt tagcttgatc ctctttgtgg ttagtatagc ctgcctcaac taaaattcca
    19501 tgaacaattt cgtggataag tgtttgatct tcgagctgct cggttaatga gtcatctagt
    19561 ttaatttgtt gtgttttgta gtgtataaga ccccaattgc catctttgcc ttacaggtcc
    19621 gatgcctttt caacagagta tgtcaaccat ccgattttta cgctttccat acctggttta
    19681 ctttcacgat tcatgtttcc tcctgttttt gggtacaaaa aaagacgggc gaccacagtc
    19741 ttccgtctat aataatatgt ttttattttg ataataaatt acgctttcta acctctttag
    19801 ataactcttt agcaaatctt tcagacacta aagcttcact cattggctga taaagcaatt
    19861 cttcatcact cattgcacta aagtcaggaa ttggatgaga ttcatcccac tctttttgtt
    19921 ttttattcaa aaaatcagaa aaatccatcg cttactcctt tcttatttcc atagtgattt
    19981 ctatttcttc atattcattt atcttcgcat taattatatc atactttgtc cctcgtggta
    20041 gtataatttc actttcttcc tcattatctg taagataaac atggcttcct ttaggtactt
    20101 ttatttcagt cttaatagga cgatatccca tcacgttttt tttaacatca taacttgcag
    20161 aagtatatcc atcgttggtg tatgataatc ctccctggtt taatttatcc ataaaattac
    20221 ctgaatcaaa aatattattg ttttgttcgt tgattgaacc taataactca aagtcatcaa
    20281 agcggtatac ttttatattt ttgggagttg aatttatcga gatgacacta tccaatgtgt
    20341 cggtaattag tttatcatca acgtttagat aattgtagcc tttatttcgc aaggtattgt
    20401 ttatatcaaa cgcatgcggt gttctaatgt acccggtatc tgctaaatca gcagagtaca
    20461 ctattttacg ttgcaaatct gttattttac ttgcaaactt actaaataac ggcatgagat
    20521 tcggtgttat gttttcaaat ttttcagatt tatcattcac tttttcttct tgtttcttat
    20581 ccaatcctac aactgcacaa cgacaatacg gatgaaacgg cggagagttt tcccctatta
    20641 tccacttgtc aaggtgatat ggaccatgct tagctatatc tttgcaaaca tcacatgctt
    20701 taggctcagg caatatatca aacatagcaa agccattctc cgccatggat aatctaccaa
    20761 cttccatctg aacccttgca tgctccgtta tcgctagtcg ctttgcgtaa gacgctgaca
    20821 catcaaattc tcgtctgata tcccttgcaa tagttaagcc atttttacct cttaggatag
    20881 cactctgaac actttgagat atgatactgc gtaacttatc ttgtctgtcc caaatgttat
    20941 ctgaccattt tgctcctttg aaattagcat tgataacggc atcagctaag cttttcacac
    21001 tcgcttggct agcaactgat aatcctaata gcccagcttg aaactcggtt tctttgcgat
    21061 agccttcatc aagaaatttc ttggttagct tgtgttcgcc gttggcaagt tcttgcattt
    21121 ctaaatcaat gtttaagcgc aatagttcca aagcattcgt tttcatggct aagttgtaaa
    21181 tagacatgtc tgcattttct tggtgggtaa aatcatctct tgttaccgtt ctcccctctt
    21241 ttcgtagctt ctcagccttt gcaacaagtt ccttagcttt cttctggtaa gcagaaatat
    21301 caagctcaga agctctctta cgggcttctg aaaggtctat tttctctttg tcagcataac
    21361 gttgataaaa cgactcaatt tctttttcga tattcctgaa atgataatcg tgcaattgct
    21421 tcatggattg ctcaagagag atatcgtctg cctctttagc atccatttct ttcttgatac
    21481 ggtcacgcca gtatttactc tttctggtgg tcatgagcta actccctatc agtcaatcta
    21541 ctggtttctt ctaacttctg cgctagttga ctacgtgatt tcgactcgct gttgataagg
    21601 ctaatttctt tcttagcatc ttccacgatg tctgtaatag ccatagctgt ctcttgcgac
    21661 aactctccac ccaatgcttt aaaggcttca atcttttctt gtagcgactt aggtaggttt
    21721 ggcgtaaatg tgattttaag cttgctgatg tcaaaatcat caatctcttt aagcagttgg
    21781 cttacacgag cgattagttt gtacctacgt ttaagagatt gctcaaataa agcttgcatg
    21841 tcgacacgct cctgatcaag accaaacact ttccacttca acgcctctcc ggattgttga
    21901 ccggcaaact tactgtctgt catgtctggc gtattggtaa atttatggat gtcggacaca
    21961 atacgatttt tataggcttc ggttccttgt acgtcatatt gtttatatag atatttggca
    22021 tctacagatc cctcacgccc gtcttggtcg acaggaggct cgaggttaag caatctagct
    22081 ttacgcatct tacgtaagta ttcaatggct ttttcggcag tgtcgacata gccagggaat
    22141 gatacacgac caatgattgc cagaatcgcg tctgacaaat cctgcatgta gttagctgtg
    22201 tcagactgcg ctgcatcata caagtcgatt aaagacaact ctgtctcgta gtcacccatg
    22261 ccgtcatctg tgttgaggta ctccgtgatt ggcaccgaac caaacgcatg agaggaagtc
    22321 tctccgatag gtgttaaatc accatcatat tcaaacttca ggattttatt atcggtgtaa
    22381 agctcgacaa tttttgtagt accatctaat tgatttttgt tatagtaccg cacaccagct
    22441 aagcttgatt gctctaggtt attttgatag ataacaaata cttcccgtgg atctagtcta
    22501 acgacctctg tcttgtcatc catgctgcga taaatcaatt caaacgctcg accaaccttg
    22561 gataggtctt ttactaatct gcggttcagc tggtggaaat tgtttttaac agataggtcc
    22621 tttaataggt cttgctgctg ctcggtacca tcaatatact ctacacgaat aggattaccc
    22681 accaaatagc cctgtttgag cgttgaaata tacttaccat agttatgcac agcacgaaca
    22741 tcagccatgt catcgtcttt cctacggcct atttcgctga tggtgtggtt atttccctct
    22801 gcataatcta gaagctcttg tatgcgtggt ttttggactt tatcgtggtg ttcaatcatt
    22861 tcacggagta gcttatattt ttcggttaac aagctttcta gactttccgc ttgatacctc
    22921 atgcgagctt cacggtggaa cctaaactct aatgttttag tctttcccgt actatctcta
    22981 aatgattctg tgtacattat tttcctttct agtaaccaaa accagcccta agaacttcaa
    23041 actgatttga ctcgttgctt ctgatatggt atttttcaag cgcatatcta atcgcatcaa
    23101 taacgtggtt attcttatct atcggttcgt ttaaccaatt accttctttg tcttgcttaa
    23161 aagtgtaagt attaaactct tctattgtgt gttcgcaaga tgggtgaata tatatcttaa
    23221 acccctgcat gaattgaatc ccttgcatga ttgacccttt acctttaata ctcgggacaa
    23281 ttccagacac ccctttactt tttatttctg caatgaggcg tttttcggcg ctatcccctg
    23341 cgatgtaaga cctatgcaag tttttatctc ttatcatttt gacaatatga tctgttaaca
    23401 tagccttttg ataatgttcg ttgtaaagcc ataactcttt gtttgcgagg tcaactgcaa
    23461 cacatataag agttgtaggg tcttgagtaa acccaaagtc cataccggcc gaggtctctt
    23521 taacgcgttg aattgttttt tcaacatcaa aatctacgac ttcaaagtta tcaaaaacaa
    23581 gaccttcagc aacgccccat tcaccatcgc acacgattct agcccgcctt ggattagtct
    23641 tgtacaaatc ttcgtagcgt cttttatcga catcatcaag ccattcgttt actctaaatg
    23701 ttgtagtccc agaaaatgta tcagcccgtt tagtttcttc atcaaaaaag acacgtttaa
    23761 gccaatgtct ttctgaccac gggttaaacg tgactgttat ctgtttaaaa aaatcaggag
    23821 catctaaact accgcggatt gattcgacaa ctgttgaaaa cttatcttcg gtctcaattt
    23881 gataagcctc ttcaaaccaa gcccagcaca aagcgccaac atcgacagta atagatgtga
    23941 tttttaactc atcatcaagt ccacggaaca gtatcttttg gcccgttgcc tttacagtta
    24001 tttctggcaa actctcatta aacttaaaaa ggtgtgtaac ctttaattga ttacacgccc
    24061 atttaaaatc ggtataagta gattgtttgt tagtgtttga gtatctacgg atgaccaata
    24121 agttagccca agggtacttc agcagtctga cgataaaatt taaagcagtc gttttagatt
    24181 ttttagatcc acggctgcct ttaactactc gataaaaatt tctagaccgc caaaaagcgc
    24241 cataacctat accgattgtt gatggcaaat ctactttaat cagggatgtt gtcttcattt
    24301 gcaaacacca ccgttccaga atgctctaat tcacgttttt ctgagtaagc tccattaact
    24361 ttataaattc tatccctagc cctaccctgt tcttcgtgac ttggggtata ttctcttttc
    24421 gtgtggataa ccacttgacc tgtcagcata tctgtttctt tgaattcaga tatttggatt
    24481 tctccacggg ctatcgatgt atcttgttcc aacgtttctg caacatcttg aatacggtct
    24541 cctttagtat cttttgcctc caaaaaacgt tccttcattg ctttttgaat ttcaacattt
    24601 ttcaacaagc gttgaccttg gctataagcg cttttttcag aatatccagc ttgttttgca
    24661 gactcagtag cgttaaatgt ggtgatgtac ttatccacaa aacgctgttg tttttcggtt
    24721 aattcctcgt atgcgatttt ccaccacctc caatccataa taaaaagcca ccacaatgtg
    24781 atgacctatt tatttttgaa attattataa gtttcaacta acaaaattaa tgttcttagc
    24841 acaaagaacg aacttaggaa catcattact gttgagctca accaacttga aactactaaa
    24901 attgaaatta aatcagtatc agtcttacta aagaatagag aaataaaaga taataaagaa
    24961 ctaatgaaag ataaaaaagt agcaactaat aggcggtcca ttatttttat ctctgtccct
    25021 aaatcttcca tcatcactac taatttatta gaaccggaag ttggtatcaa tgaaaaacta
    25081 gcaaagaata ttgcggttac aattgctgaa aatgatagag atgccgacat tatatcagta
    25141 aaatttttta aactttttgg agaaatttta aaataaaaac taaatataaa aactattata
    25201 ccaaatagta taatcggcca gtttctttta aaccttcctt ttttcatcgc atacctcaat
    25261 ataagttata aagttatagt aaacattctt tttaaatg
//
LOCUS       DAUDYX010000025        25247 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.025, whole genome shotgun sequence.
ACCESSION   DAUDYX010000025 DAUDYX010000000
VERSION     DAUDYX010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25247
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.025"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            226..474
                     /locus_tag="VHD02_001019"
     CDS             226..474
                     /locus_tag="VHD02_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEQ5497637.1"
                     /translation="MGLIWTLIVGALIGVIAGALTKKGGSMGWIANIAAGLVGSSVGQ
                     ALLGSWGPSLAGMSLIPSVIGAVIVVMITSFVLNKTNN"
     gene            511..1122
                     /gene="amaP"
                     /locus_tag="VHD02_001020"
     CDS             511..1122
                     /gene="amaP"
                     /locus_tag="VHD02_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEQ5497638.1"
                     /translation="MLSRKEVCMSKLLKISYCLVGLVLLSVFGWVVGITGGYIYLPYS
                     YRWLSWGMDSFPNLLDSALSYYYFWTALVLFVITFLALLVIILYPRIYTEVHLRHKNK
                     KGTLLLKKSAIESYVATAIQTAGLMPNPTVTAKLYKRKFKIIVKGRLASRVAVADQIS
                     GVKEGIEKGITEFFGINYPVNFKVYVKDIADSDRKHITRNRVE"
     gene            1133..1321
                     /locus_tag="VHD02_001021"
     CDS             1133..1321
                     /locus_tag="VHD02_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEQ5497639.1"
                     /translation="MEFYEKFKYPIIGGLVGLIIAILLMAFGLFKTLLAIIFIILGIY
                     GGLYAKKTGIIDQFLNRK"
     gene            1334..1873
                     /locus_tag="VHD02_001022"
     CDS             1334..1873
                     /locus_tag="VHD02_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ5497640.1"
                     /translation="MTETYIKNTSKDLTSAIRGQLTYDDKVIEKIVGLALENVDGLLG
                     VNGGFFANLKDKLVNTESVRDGVNVEVGKKQVAVDLDIVAEYQKHVPTIYDSIKSIVE
                     EEVKRMTDLDVIEVNVKVVDIKTKEQFEAEKVSLQDKVSDMARSTSEFTSHQVENVKA
                     SVDNGVEKLQDQKAEPRVK"
     gene            1914..2114
                     /locus_tag="VHD02_001023"
     CDS             1914..2114
                     /locus_tag="VHD02_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEQ5497641.1"
                     /translation="MSDEKYNAKLDQAGGKLKEGFGKISGDKSLETEGKVDKVTGKIK
                     EVIADAKDTVKGLAKGLDNKDK"
     gene            2125..2613
                     /locus_tag="VHD02_001024"
     CDS             2125..2613
                     /locus_tag="VHD02_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ5497642.1"
                     /translation="MEKTQIKSTLTYDDKVIEKIVGHALENVGGLLAVTGGFFSNIKN
                     NLVNSESVTDGVSVEVGSKEVAVDLAIIVEYGKDIPAIAESIKAIVSQNVDSMTHLKV
                     VEVNVNVVDIRTKEEHEAASVTVQDRVTSAASSTSQFVSEQTEKLKDTISDTVNSDEA
                     AK"
     gene            2776..3417
                     /locus_tag="VHD02_001025"
     CDS             2776..3417
                     /locus_tag="VHD02_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ5497643.1"
                     /translation="MISPKKEITSSKYQKIAISVAQRIANGEYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLKILTLKHGSGAIVLSKERAIEFINQYESTHSIAVLKEKIR
                     ETINDQGKAMEKMAVLVSDFLMQSQSVSKQYPLAPYEIICSQDSEHFGKSIGVLNIWH
                     QTGATIVAIEHAGQFIVSPGPYSVIEKGDHIYFVGDESVISRMKTFFNLRKGL"
     gene            complement(3560..4102)
                     /locus_tag="VHD02_001026"
     CDS             complement(3560..4102)
                     /locus_tag="VHD02_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ5497644.1"
                     /translation="MTKRHTETKAYVKTALTTLLTEQSFETLTVSDLTKKAGINRGTF
                     YLHYTDKFDMMNHFKNDTLDDLYRLLNQAKIYTDTRQVLNQTLSYLIEHREFITALAT
                     ISYLKFPQLIKDFCYQFLTTITGFQDIVTNQYHIPYPYALEVYLASIESIISYWIANG
                     YQETTEEVADIILKAVTLDN"
     gene            4220..4921
                     /locus_tag="VHD02_001027"
     CDS             4220..4921
                     /locus_tag="VHD02_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5497645.1"
                     /translation="MAFIELKQVSKSYQVGETTVFANHEVSFEINKGELVVILGASGA
                     GKSTVLNILGGMDTVDAGQVIIDGKDIAHYTSKALTQYRRNAIGFVFQFYNLVPNLTA
                     KENVELAVEIVADALDPVTILKEVGLSHRLDHFPAQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDYQTGKQILTLLQDMARTKGTTVVIVTHNAAIAPIADRVIFMHDAQVTK
                     TVINKEPASIETIDY"
     gene            4936..7572
                     /locus_tag="VHD02_001028"
     CDS             4936..7572
                     /locus_tag="VHD02_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEQ5497646.1"
                     /translation="MIKKTLWKDILRAIKNSKGRFISLFFLMALGSFALVGLKVTGPD
                     MERTASRYLERHQVMDLTVLASHQFSQADKQELDTLKGAHLEYGHLLDVSLTSNQKSL
                     RLYSVPKKVSKPVLVKGSWPKRETDLVLSSSLAKNYQIGDELAVTSPMEGLLTTTHFQ
                     VVGFANSSEVWSKSNLGSSSTGDGSLYAYAFVNPNVFKSAFNLLRIRFSNLRLTNAFS
                     KDYQKRVTQNQAHLDNLLKDNGQKRYDDLQNQYDLALKNGRAALAKETVKLAASEENL
                     TFLEGSALQEAKHQIEQGKQALAKEEKQLEQVQATKDKLEKPSYLTYNRSTLPGGEGY
                     HTYATSTTSISNVGNIFPVVLYLVAALVAFTTMTRYVDEERTSSGLLKAIGYSNKDIS
                     LKFLIYGLLASFLGTTLGIIGGTYLLSALISEILTGALTIGKTHLYSYWFYNGIAYLL
                     AMLSAVLPAYLIVKKELFLNAAQLLLPKPPSKGAKIWLEHLTFVWKALSFTHKVTIRN
                     IFRYKQRMLMTIVGVAGSVALLFAGLGIQSSLAKVVEHQFGDLTTYDILAVGSAKATA
                     TEQTDLASYLKQEPITGYQKVSYASLTLPVKGLPDKQSISILSSSATSLSPYFNLLDS
                     QEQKKVPIPTSGVLISEKLASYYKVKPGDQLVLTDRKGQSYKVTIKQVIDMTVGHYLI
                     MSNTYFKNHFKGLEAAPAYLIKVKDKDSKHIKETASDLLTLKAIRAVSQNANHIKSVQ
                     LVVTSLNQVMTLLVFLSILLAIVILYNLTTINIAERIRELSTIKVLGFYDQEVTLYIY
                     RETISLSLVGILLGIYLGKGLHTYIMTMISTGDIQFGVKVDAYVYLVPILVILSLLAV
                     LGIWVNRHLKKVDMLEALKSID"
     gene            7664..8260
                     /locus_tag="VHD02_001029"
     CDS             7664..8260
                     /locus_tag="VHD02_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEQ5497647.1"
                     /translation="MENSIEAYLADSREKVTLGTVVDCFKGKAVSSKVVPGDVGLINL
                     SDMGTLGIQYHQLRTFQMDRRQLLRYLLEDGDVLIASKGTLKKVCVFHKQNRDVVASS
                     NITVLRPQKLLRGYYIKFFLDSPIGQALLDAVDHGKDVINLSTKELLDIPIPVIPLVK
                     QDYLINHYLRGLTDYHRKLNRAEQEWEYIQNEIQKGLG"
     gene            8271..9227
                     /locus_tag="VHD02_001030"
     CDS             8271..9227
                     /locus_tag="VHD02_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEQ5497648.1"
                     /translation="MGSVERTLKMTLATIVAILIAYQLHLDYAMSAGIIALLSVLDTR
                     KSSLVVARNRLLSFFLAFGIAMMCFSLFGFTTVGFMCYLLIIIPLLYHFQIEAGLVPI
                     TVLVTHLIAKKSIALPILSNEFMLFFVGTSVALLFNAYMGPQDQQIRYYHQKVESDLK
                     GILYRFESFLLEGKGQNEGLLIKNLDKILDEALKLVYRERHNQLFQQTNYQVHYFEMR
                     RQQNRLLGQMAINVNTLMRQSKESILLSHLFHETACQLSEQNPALTLIDDIEQLLETF
                     RHGDLPQTREEFERRAVLFQLLQDLERFILLKVEFYQDYQND"
     gene            9324..10664
                     /locus_tag="VHD02_001031"
     CDS             9324..10664
                     /locus_tag="VHD02_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEQ5497649.1"
                     /translation="MNEIKCPHCHTLFTINESEYSQLLEQVRGQAFDEELQKRLINEI
                     ALLEEKAKHQLHEVVAKKETAITSLTNQLEQIEKEQAYLRQEELAKKDQLIASLEAKL
                     DKLASQNALELANQLAEKDKEVISLTNQLDKLALEKDATFHSKLATIEKERDGIKNQL
                     VLQAKESELSLASVRSDYEAQLKAANEQVEFYKNFKAQQSTKAIGESLELYAETEFNK
                     VRSYAFPNASFVKDNQLSSRGSKGDYIYREVDTNGVEILSIMFEMKNEADTTKTKHKN
                     SDFFKELDKDRREKDCEYAVLVSMLEADNDYYNTGIVDVSHEYQKMYVVRPQLFIQLI
                     GILRNAALNSLHYKQELALIKEQNIDITHFEEDLDQFKNAFAKNYQSASNNFKKAIDE
                     IDKSIKRMEEVKRFLTTSENQLRLANNKLEDVSVKKLTRQNPTMREKFEALKDQ"
     gene            10780..11664
                     /gene="truB"
                     /locus_tag="VHD02_001032"
     CDS             10780..11664
                     /gene="truB"
                     /locus_tag="VHD02_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEQ5497650.1"
                     /translation="MINGIINLKKEAGMTSHDAVFKLRKLLQEKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTEAGKVYEGQVTLGYSTTTEDASGEVVARSSLPAVLTEELVD
                     QTMTTFLGKITQTPPMYSAVKVNGRKLYEYARAGESVERPRREVTISLFERTSPLNFT
                     EDGLCRFSFKVACSKGTYVRTLAVDLGRALGVESHMSFLQRSASAGLTLETAYTLGEI
                     ADMVSKQEMSFLLPIEYGVADLPKMVIDDTELTEISFGRRLSLPSQEPLLAAFHGEKV
                     IAILEKRDQEYKPKKVLI"
     gene            11693..12625
                     /locus_tag="VHD02_001033"
     CDS             11693..12625
                     /locus_tag="VHD02_001033"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEQ5497651.1"
                     /translation="MEIEYIKDYRDINQEDDTVLILGYFDGLHRGHKALFDKAREVAN
                     KEGLKVVVFTFTESPKLAFSRFSPELLLHITYPKKRYEKFADYGVNKLYLVDFTSKFS
                     KVSSDHFITHYIKNLKAKHIVVGFDYKFGHNRTDSDYLTRNFEGQVYTIEEIKEDHRK
                     ISATWIRKLIQEGNVVKANHLLGYDLSTRGRVVHGDARGRTIGFPTANLAPIDNTYLP
                     ADGVYVTNVIVANKIYRSMTSLGKNVTFGGKELRLEVNIFDFDEEIYGEIIEIVWLDK
                     IRDMEKFEGIEDLTDRLEYDKRTALNWKKDSKLS"
     gene            12668..13072
                     /locus_tag="VHD02_001034"
     CDS             12668..13072
                     /locus_tag="VHD02_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEQ5497652.1"
                     /translation="MVTLFLSPSCTSCRKARAWLVKHEVDFQEHNIITSPLSRDELMS
                     ILSFTENGTEDIISTRSKVFQKLDVDVEELSISDLIDLIAKNPSLLRRPIIMDQKRMQ
                     IGFNEDEIRAFLSRDYRKQELRQATIKAEIEG"
     gene            13074..13351
                     /locus_tag="VHD02_001035"
                     /pseudo
     CDS             13074..13351
                     /locus_tag="VHD02_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
     gene            13341..14129
                     /locus_tag="VHD02_001036"
     CDS             13341..14129
                     /locus_tag="VHD02_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEQ5497653.1"
                     /translation="METKYAFARQIIKEAGLFIKSKMSEQLDIQVKTQFDDLVTNVDQ
                     ETQQLLMDRIHQTYPCDAILAEENDVRHPINQGNVWVIDPIDGTVNFIVQGSQFAVMI
                     AYYEQGIGQFGLIYDVMADQLLAGGGDFEVTLNGDKLPAYQEKPLERSLIGCNAGMFA
                     RNDRNLAHLIAKTLGVRVYGGAGICMVKVMKQELLAYFSFIQPWDYAAAKVLGDKLGY
                     VLLTIDGYEPDFQTRQKIMFVPKCQLTRIASFLTKDSAVTLEDQ"
     gene            14132..15442
                     /locus_tag="VHD02_001037"
     CDS             14132..15442
                     /locus_tag="VHD02_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEQ5497654.1"
                     /translation="MMSLPKEFINTYQAILGKELEDFLASFNQEPVNAFRINPLKNQL
                     KTFEHPIPNTLWGYYGKLSGKSQEHVSGLVYSQEPAAQMVAQVAAPQKGSRVLDLAAA
                     PGGKSTHLLAYLDNTGLLVSNEISKKRSKVLVENIERFGARNVVVTNESADRLAKVFS
                     HYFDTIVFDGPCSGEGMFRKDPDAIQYWHHGYPAECAKLQKSILEDALTMLKPGGELI
                     YSTCTWAPEENEDVVQWLLETYTFLELVDVPKLNGMVSGIGLPETARMYPHRYQGEGQ
                     FVAKLKDKRQEGQSTKLKAPKSNLTKDQLRLWKMFEKDHLNLTLSGTLQTFGDYLYLL
                     PDGLPALDGLKIARNGLELGVFKKKRFEPSYALGLALKPDEVRSSIELREEDFRPYVS
                     GNVITLPQSYPTGWYQLLINGNGLGFAKVVDKTIKNNFPKGLRF"
     gene            15581..16447
                     /locus_tag="VHD02_001038"
     CDS             15581..16447
                     /locus_tag="VHD02_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEQ5497655.1"
                     /translation="MKMKKKFFLLSLLVLSTFFLSACSSWIDKGESITAVGSTALQPL
                     VEAVADEFGSSNLGKTVNVQGGGSGTGLSQVQSGAVQIGNSDVFAEEKDGIDASKLVD
                     HQVAVAGLAVIANPKVKVSNLSSQQLQKIFSGEYTNWKQVGGEDLAISVINRAASSGS
                     RATFDSVIMKGVNAKQSQEQDSNGMVKSIVSQTPGAISYLSFAYVDSSVKSLQLNGFK
                     ANAKNVATNDWPIWSYEHMYTKDKPTGLTKEFLDYMFSDEVQQNIVTHMGYISINDME
                     VVKSHDGKVTKR"
     gene            16458..17393
                     /gene="pstC"
                     /locus_tag="VHD02_001039"
     CDS             16458..17393
                     /gene="pstC"
                     /locus_tag="VHD02_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEQ5497656.1"
                     /translation="MENQELAKKLASPSKNSRLETFGRTITFLCLALIVFIVAMILIF
                     VAQKGLSTFFVDKVNLFDFLLGKEWQPSVKNAAGIPYLGALPMITGSFLVTILSAIIA
                     TPFAIGAAVFMTEISPKYGAKLLQPAVELLVGIPSVVYGFIGLQVIVPFMRSIFGGTG
                     FGILSGVCVLFVMILPTVTFMTTDSLRAVPRHYREASMAMGATRWQTIWRVVLNAARP
                     GIFTAVIFGMARAFGEALAIQMVVGNSAVMPSSLTTPAATLTSVLTMGIGNTVMGTVQ
                     NNVLWSLALVLLLMSLAFNSLVKLITKERKRNYER"
     gene            17383..18270
                     /gene="pstA"
                     /locus_tag="VHD02_001040"
     CDS             17383..18270
                     /gene="pstA"
                     /locus_tag="VHD02_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEQ5497657.1"
                     /translation="MNAKKVDKVATGTLYTIAGIIVAILASLILYILVRGLPHISWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSTGAGIYLAEYAKKGPVTNFIRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLLHVH
                     HTQREAGLALGLSRWETVFYVVIPEALPGMVTGIVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNPLSVTSPISIFRQSETLAVHIWKVNSEGTIPDATLVSAGSAAVLLIFILIF
                     NFSARFIGKKLHSKMTAAK"
     gene            18286..19089
                     /gene="pstB"
                     /locus_tag="VHD02_001041"
     CDS             18286..19089
                     /gene="pstB"
                     /locus_tag="VHD02_001041"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEQ5497658.1"
                     /translation="MTEYNWNERHIITFPEETLALATKDLHVYYGAKEAIKGIDMQFE
                     KHKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGEILYQGIDVNRKDMNVYEIRKH
                     LGMVFQRPNPFAKSIYKNITFAHERAGVKDKKVLDEIVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGNLIEYDKTRNIFQNAQCQSTNDYVSGHFG"
     gene            19102..19860
                     /gene="pstB"
                     /locus_tag="VHD02_001042"
     CDS             19102..19860
                     /gene="pstB"
                     /locus_tag="VHD02_001042"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEQ5497659.1"
                     /translation="MTESILQIRDLSVYYNQKKTLKDVSLDLYPNEITALIGPSGSGK
                     STLLRSINRMNDLNPEVTITGSIVYNGHNIYSPRTDTVDLRKEIGMVFQQPNPFPMSI
                     YENVVYGLRLKGIRDKSILDHAVESSLKGASIWNEVKDRLHDSAVGLSGGQQQRVCIA
                     RVLATSPRIILLDEPTSALDPISAGKIEETLLLLKKDYTLAIVTRSMQQASRLSDRTG
                     FFLEGDLLECGPTKAMFMNPKRKETEDYISGKFG"
     gene            19928..20581
                     /gene="phoU"
                     /locus_tag="VHD02_001043"
     CDS             19928..20581
                     /gene="phoU"
                     /locus_tag="VHD02_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEQ5497660.1"
                     /translation="MLRTKFEEELDKLHNQFYSMGTEVLAQINKTVRAFVSHDRELAK
                     EVIEEDDTINNFETKLEKKSLEIIALQQPVSNDLRMVITVLKASSDIERMGDHAASIA
                     KATIRMKGEERIPVVEEQINLMGKAVKQMVEEALNAYINADDTKAYEIAASDEIIDQY
                     FRNIQTLAVEEIRKSPDAVFAGKEYFQVLMYLERIGDYARNICEWIVYLKTGKIIEL"
     gene            20786..23323
                     /locus_tag="VHD02_001044"
     CDS             20786..23323
                     /locus_tag="VHD02_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEQ5497661.1"
                     /translation="MKTVEHLIETFVPENYNIFLDINRQTKTFTGNVAINGEALDNHV
                     AFHQKDLDIKSILLDNEAVIYQVDNDNEVVRVELPETGMMTLVIEFSGSITDNMTGIY
                     PSYYTKNGEKKEVISTQFESHFAREAFPCIDEPQAKATFDLSLTFDQEIGEIALSNMP
                     EVNIDRREETGLWTFDTTPRMSSYLLAFALGELHGKTVESKKGTTVGVYATTAHPLSS
                     LDFSLDIAVRVINFYEDYFGVHYPIPQSLNIALPDFSSGAMENWGLITYREIYLLVDE
                     NSTVQSRQQVALVIAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIEAIEPSWK
                     IIEDFQTGGIPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRF
                     IGDRDFAIGLHHYFEKYQYRNTVGRDLWNILSDTSGKDVAAFMDAWLEQPGYPVLTAR
                     LENDQLILSQKQFFIGKGEEKGRLWPIPLNTNWHGLPETLTEAEMVIPNFSQLAAENE
                     GALRFNIGNTAHYITDYQGSLLDALVTELAQLDNTSALQVIQERRLLADSGLISYAEL
                     VDLIAQLDDSKSYMVAEAVQQVVSGLKRFIDEGSLAEKSFNRLVTTIYQEDFNHHGFE
                     KKADESDEDEMVRQVALGRLWLAENQTIIDGLRTIFEAYQSNIASIPAAVRRLVLANQ
                     MKYFETDSLVDIYFETYVATTDNNLRSDLTVAFSQTKQSTTIRRILVSLKDKDIIKPQ
                     DLSYWYNALLGQSFTQDIIWEWARENWDWIKSALGGDMSFDKFVIYPASNFKTPKHLA
                     EYKSFFEPKLDDMAISRNITMGINEIEARVALITKEKEAVIAALSHY"
     gene            23669..24343
                     /locus_tag="VHD02_001045"
     CDS             23669..24343
                     /locus_tag="VHD02_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ5497662.1"
                     /translation="MIKILLVEDDLSLSNSIFDFLDDFADVMQVFDGDEGLYEAESGI
                     YDLILLDLMLPEKNGFQVLKELREKDIKIPVLIMTAKEGLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRTGKFADKNISFGNLVVDLARKEVKVEGKVVELLGKEFDLLVY
                     LLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYISKIRKKLKGTRFVNRLQTLRSVGY
                     ILKNNE"
     gene            24336..>25247
                     /locus_tag="VHD02_001046"
     CDS             24336..>25247
                     /locus_tag="VHD02_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ5497663.1"
                     /translation="MNKLKKEILSDNYNHFFHFFAVFTGIFVIMTIIILQIMRFGVYS
                     SVDSSLVSVSNNASSYANRTMARISSFYFDTENNIIKALPDSDSSKLLGTPAANTDII
                     LFSANGTILNAFDAFSNYQNFHLDKYRLGSIETTSLMNFYGQEEKYHTITVRVHIKNY
                     PAVAYMMAVVNVEQLDRANERYERIIIIVMSVFWLISILASIYLAKWSRKPILESYEK
                     QKMFVENASHELRTPLAVLQNRLESLFRKPNETILENSEHLASSLDEVRNMRILTTNL
                     LNLARRDDGINPQWTHLDTDFFNAIFEN"
BASE COUNT         8018 a         4169 c         5188 g         7872 t
ORIGIN      
        1 tataaattaa ataacgagtg gtcactttat aaaatattat caaaataaca ttttatagga
       61 ttttgaaata tcggtattca aacaaaaaat aaactaacac tttattattt agtttatata
      121 gtcacttttt gaaagtcgac tattaaaaga ctattgcttg aagaataaca gctgttattc
      181 ttccttggac aaaaggtaca ggagtatcta aaaaggagaa ttatcatggg acttatttgg
      241 actttaatcg tcggtgcgct tattggtgtg attgctggag cacttactaa aaaaggtggt
      301 tcaatgggct ggattgcaaa catcgctgct ggtctagttg gttcttccgt tggtcaagct
      361 ttacttggtt catggggccc ttctttagca ggaatgtctt tgattccgtc agttattggt
      421 gccgttatcg ttgttatgat cacttctttt gtactaaaca aaactaacaa ctagtttcct
      481 ctttatttag taagtactta caaatagaga atgttaagta gaaaagaggt ctgtatgtca
      541 aaattgttaa agattagtta ttgtcttgtc ggacttgttc tattgtctgt ttttggatgg
      601 gtagttggaa taaccggagg ctatatatat ttaccttata gttatcgttg gctaagttgg
      661 ggtatggata gttttcccaa tctcttagat tcagctttat cctattatta cttctggaca
      721 gcccttgtat tatttgttat aacatttctt gccttattag ttattatttt atacccaaga
      781 atttatactg aggtccattt acgccataaa aataaaaaag gaacactttt attaaaaaaa
      841 tcggctattg aaagttatgt tgcgaccgct attcaaactg caggtttgat gccaaaccca
      901 acagtcacag ctaaattata taagcggaag tttaagatta tcgttaaagg acgtttagct
      961 tctcgtgtag cagtggcaga tcaaattagt ggcgtgaaag aagggatcga aaaaggtata
     1021 accgagttct tcggaattaa ttatcctgtt aatttcaaag tttatgttaa agatattgct
     1081 gattctgatc gtaaacatat taccagaaat cgtgtagaat aggagatcta tcatggaatt
     1141 ttatgaaaaa ttcaagtacc ctattattgg tggcttggtg gggctaatta ttgccatatt
     1201 gctcatggct tttggtttat tcaaaacgct actagcaatt attttcatta ttttaggaat
     1261 atatggtggc ctgtatgcca aaaaaacagg tattattgat caatttctta atcgtaaata
     1321 aaggagactt attatgactg aaacttatat caaaaacaca tctaaagact taacttcagc
     1381 aattcgtggc caattaacat atgatgataa agtaattgaa aaaattgttg gtttagcact
     1441 tgaaaatgtt gatgggttac tgggtgtaaa tggtggtttt tttgctaacc taaaagataa
     1501 attagtaaac accgaatctg tacgtgatgg cgtgaacgtg gaagttggaa aaaaacaagt
     1561 tgccgttgat cttgatattg tcgctgaata tcaaaagcat gtgccgacta tttatgactc
     1621 tattaaatcg atcgttgaag aagaagtgaa gagaatgacc gatctggatg tgattgaagt
     1681 taatgttaaa gtagtcgaca ttaaaacaaa agaacaattt gaagctgaaa aagtaagttt
     1741 acaagacaaa gtttcagaca tggcacgttc aacatctgaa tttacgagtc atcaagttga
     1801 gaatgttaaa gcttcagtgg ataacggtgt tgaaaaactt caagatcaaa aagctgagcc
     1861 acgtgtaaaa taatcatatg tcgtcaatta ggtaagctaa aggagggttt attgtgtcag
     1921 atgaaaaata taatgcgaaa ttagatcaag ctggtggcaa gttaaaagaa ggatttggta
     1981 agatttctgg agataaatct ttagaaactg aaggtaaagt agataaagtt actggaaaaa
     2041 ttaaagaggt tattgcagat gccaaagata ctgtaaaagg attagctaaa ggcttagata
     2101 ataaagataa ataaagggga catgatggaa aaaactcaaa ttaaaagcac tcttacttat
     2161 gacgataagg tcatcgaaaa aattgtcggt catgctttgg aaaacgttgg cggattacta
     2221 gctgtaacag gtggattttt ctcaaatatc aaaaataatt tggtgaattc tgagtctgta
     2281 actgatggtg tttcagttga agttggtagc aaggaagtag cagttgattt agcaatcatt
     2341 gtagaatacg gtaaagatat tcctgctatt gcagaatcta ttaaagctat tgtttctcaa
     2401 aacgttgaca gtatgacaca tttgaaagtt gttgaagtca atgtgaacgt tgttgatatt
     2461 cgtactaagg aagaacatga agctgctagc gttactgttc aagatcgtgt cactagtgca
     2521 gcttcaagta cttcacaatt tgtatcagaa caaacagaaa aattaaaaga tactatctct
     2581 gatactgtaa atagtgatga ggcagctaaa taagcaatcg tctaggaagt attattgact
     2641 agtggtgata agtttgagct ggtatttacc agctcttttt gtcacggtta aaaagatacg
     2701 gactattgtc atctctctaa aaatatgatt taattagata taaatttttc aaattgttag
     2761 aaaggagtaa attaagtgat aagtcctaaa aaagagataa caagttcaaa atatcaaaaa
     2821 attgctattt cagtagctca gcgtatcgca aatggcgaat atgaagtggg cgaaaagtta
     2881 aagtcaagaa ctactatcgc ctcgacattt aatgtatcac ccgaaacggc tcgtaaaggc
     2941 ctgaatattt tagctgactt gaaaattttg actttaaaac atggtagtgg agctattgtg
     3001 ctgtcaaaag aaagagctat tgagtttata aatcaatacg aatcaaccca ttccatcgct
     3061 gttttaaaag aaaaaatacg tgagactatt aatgatcagg gaaaagcaat ggaaaaaatg
     3121 gctgttttgg tcagtgattt tttaatgcaa agtcaatctg ttagtaagca atacccatta
     3181 gcaccttatg agattatttg tagtcaagat tcggaacatt ttggcaaatc tatcggtgtg
     3241 ttaaatatct ggcaccagac aggagcaact atcgtcgcta ttgaacatgc tggtcagttt
     3301 atcgtttcac caggtcctta ttctgttatt gaaaaaggcg atcatattta tttcgttggt
     3361 gatgagtcag tcatttcacg aatgaagacc ttttttaatt tacgaaaagg attataagtt
     3421 tgagggccca cctcaaactt tttttgttgc tgttgagcat tattaaaaga ggcagatcat
     3481 catggtttgt ctttccaaaa tatctgttta gacagttgtt ttggattggg agcatgattt
     3541 cagactcttt tgatgttttt taattgtcta gagtaactgc tttgagaata atatcggcaa
     3601 cctcttcagt ggtttcttga tagccattag caatccaata gctgataatg ctctcaatac
     3661 tggcaaggta aacttctaaa gcatagggat aaggaatatg gtattgattg gtcacaatgt
     3721 cttggaaacc agtaatagta gtcaaaaatt ggtaacagaa atccttgatg agttgcggaa
     3781 atttgaggta agaaatagta gcgagtgcgg ttataaattc tctatgttct ataaggtagg
     3841 acaaggtttg attgaggact tgtcgagtgt ctgtgtagat tttagcctga tttaacaaac
     3901 ggtacaaatc atccagagtg tcatttttga agtgattcat catgtcaaat ttatcagtgt
     3961 agtggaggta gaaggttcct ctattgatac cagccttttt agtaaggtca gagacagtaa
     4021 gagtttcaaa agattgttca gttagtaagg tggttaaggc ggtctttaca taagctttgg
     4081 tttcagtatg tcttttggtc attctcaggt gctccttaaa tcaacagttg cgtcagattt
     4141 gttcttgtta aatacttagg gccattatat aataaggcta taattaaatc aacactatgt
     4201 tcaaataatg gaggggcaga tggcttttat tgaattaaaa caggtttcaa aatcttatca
     4261 ggtaggagag acgactgttt ttgccaacca cgaagtgtct tttgagatta ataaaggaga
     4321 actagtggtt attttgggag catccggagc tggtaaatcg acagtcctca atattttggg
     4381 gggcatggac acagtagatg caggtcaagt aattattgat ggcaaagaca ttgctcatta
     4441 cacgtctaag gccttaactc agtatcgtcg gaatgctatt ggtttcgttt ttcaatttta
     4501 taatttggtt cctaatttga cagctaaaga aaatgttgaa ttagcagttg aaattgtagc
     4561 agatgcttta gatcctgtga ccattttaaa ggaagtagga ctcagtcatc gtctggatca
     4621 ttttcctgct cagctctcag gtggtgaaca gcaacgggtt tcgatagcac gtgccttagc
     4681 taaaaaccct aaattgcttc tttgtgatga acctacaggt gcccttgact accaaacagg
     4741 aaagcaaatt ctaaccctct tacaggatat ggcacgaact aaggggacca cggtagttat
     4801 tgtaacccac aatgctgcta ttgctccgat agctgatcgt gtcattttta tgcatgatgc
     4861 acaggttacc aagacagtca ttaataagga acctgcaagc attgagacaa ttgattatta
     4921 agaggaaggt gtcgtatgat aaagaaaacc ttatggaagg atattttgag agctatcaag
     4981 aatagtaaag ggcgattcat ttccttattt ttcctaatgg ccttaggctc ctttgcttta
     5041 gttggactta aagtcaccgg tccagatatg gaacgaacag ccagccgtta cctcgaaaga
     5101 caccaggtga tggacctaac ggtactggct tctcatcaat tttcccaagc cgataaacaa
     5161 gagttagata cgttaaaagg ggctcattta gaatatggtc atttacttga tgtcagtcta
     5221 actagcaacc agaaatctct taggctctat agcgtgccaa agaaagtgtc taagccagtc
     5281 ttggttaaag ggagttggcc aaagcgagag acagatttgg ttttatcctc ctcacttgct
     5341 aaaaactatc agattggaga tgaactagca gtcacctcac ctatggaagg tttgctgacg
     5401 acaacgcatt ttcaagtggt tggttttgcg aattcttcag aagtttggtc caagtccaat
     5461 ttaggaagtt cctcaaccgg agatggaagt ctctacgctt atgcttttgt taatccaaac
     5521 gtttttaaaa gtgcctttaa cctgctgcgc atccgtttta gtaatcttcg tctgacaaac
     5581 gctttttcca aagactatca aaaaagggta acccaaaacc aagctcattt ggataatcta
     5641 cttaaagata atggccagaa gcgttatgat gacctccaaa atcagtatga ccttgcctta
     5701 aaaaatggca gagcagcact tgcaaaggaa acagtgaaac tagctgcgag tgaggagaac
     5761 ttaacttttt tagaaggctc tgctttacaa gaagctaagc atcagattga acaaggcaaa
     5821 caagcattag ccaaggagga aaagcagtta gagcaggtgc aggctacaaa agataagcta
     5881 gaaaaaccca gttacctgac ttataatcgc tcgaccctac caggaggaga aggatatcat
     5941 acttatgcaa cttcaacgac ctccatttca aatgttggaa atatttttcc tgttgttctt
     6001 tatctcgtag ctgctctagt agcgtttacc accatgacac gttatgttga tgaagaaaga
     6061 acaagctctg gtctattaaa agccattggt tattctaaca aggatatcag tttaaagttt
     6121 cttatctatg ggcttttagc cagtttttta gggacaactt taggtattat tgggggaact
     6181 tacctcttat ctgccttgat ttcagagatc ttaacaggag ctttgactat tggaaagact
     6241 cacctttata gttattggtt ttataatggc atagcttact tgctggctat gttatctgct
     6301 gttttaccag cctacttaat tgtcaaaaag gaattattcc tcaatgcagc tcagttattg
     6361 ctgcccaaac ctcctagtaa gggggcaaaa atctggttgg aacaccttac ttttgtctgg
     6421 aaagccctgt cctttactca caaggtgacc atacgtaata tttttcgcta taaacaaaga
     6481 atgctgatga ccattgtagg cgttgcgggc tcagtagccc ttttatttgc aggcttaggg
     6541 attcagtcgt cattagccaa agtagttgag catcaatttg gtgatttaac gacttatgat
     6601 attttggctg tcggttcggc caaagcgaca gcgacagagc aaaccgactt agctagctat
     6661 cttaaacaag aacctattac agggtaccaa aaggtatctt atgccagctt aacccttcct
     6721 gtaaagggat tacctgataa gcaaagtatt tccattttat caagttcagc tacttctctt
     6781 agcccctatt ttaatctgct ggatagtcag gagcaaaaga aggttcccat tccaacctct
     6841 ggtgttttga tttctgagaa attagcctcc tattacaagg taaaaccagg tgatcagttg
     6901 gtattgactg atcggaaagg acagtcttat aaagtgacga ttaaacaggt tattgacatg
     6961 acagttggcc attacctgat aatgtctaat acctatttta agaatcattt taaaggattg
     7021 gaggctgctc ctgcctatct gattaaggta aaagacaaag atagcaagca cataaaggag
     7081 acagccagtg acttgttaac cttaaaagcg attagagcag tttcacaaaa cgccaatcat
     7141 attaaatctg ttcagctagt agtcacctct cttaatcagg tcatgaccct ccttgtcttc
     7201 ttgtctattt tattagcaat cgttatcctt tataacttaa cgactattaa tattgctgag
     7261 cgtattcgag aattatccac tattaaagtt ctgggatttt acgatcagga ggtcacctta
     7321 tatatttatc gagaaactat ttcgctatcc ctagtaggca ttcttttagg tatctattta
     7381 ggaaaaggcc tgcatactta tatcatgaca atgatttcaa ctggggatat tcaatttggt
     7441 gtaaaggttg atgcttatgt ttatctagtg ccaattctag taatccttag cttgttagcg
     7501 gtattaggta tctgggttaa tcgccattta aaaaaggttg atatgttaga agctttgaaa
     7561 tccatagatt gattggtgaa aagacagaga tagacagcac ttgaaaagtt aatcatatta
     7621 tgatagtatc taaatactat gatttaatag aaagatacta tgtatggaaa atagcattga
     7681 ggcctatcta gcagatagca gagaaaaggt aacattaggt acagttgttg attgttttaa
     7741 aggaaaagca gtctcaagta aggttgtgcc aggtgatgtg ggattgatta atttatctga
     7801 catgggcacc ttaggaattc aatatcacca attaagaact ttccagatgg atcgtaggca
     7861 gctcttgcgt taccttttgg aagacggaga tgtcttaatc gcctctaagg gaaccctaaa
     7921 aaaagtctgt gtttttcata agcaaaatcg agatgtggtg gcatcatcaa atatcactgt
     7981 cttacgccct caaaaactgt taaggggcta ctatatcaag ttctttttgg actcacctat
     8041 tggtcaagcg ctcttggacg cagtggacca tggcaaagat gtgatcaatc tctcaacgaa
     8101 agaattgttg gatattccaa ttcccgttat tcctttagtg aaacaggact atttgattaa
     8161 tcactacttg agaggattaa cagattatca tcgtaaactc aatcgagcag aacaggagtg
     8221 ggaatatatt cagaatgaaa ttcaaaaagg attaggttag gagtcttgtc atgggttcag
     8281 ttgaacgtac attaaaaatg accttggcta ccattgttgc cattttaatt gcttaccaac
     8341 ttcatctgga ttatgcaatg tcagcgggga tcattgccct gttaagtgtt ttagacaccc
     8401 gtaagtccag tttggtggtc gctaggaatc gcttgctatc ttttttcctg gcttttggga
     8461 ttgcgatgat gtgctttagc ttgtttggtt ttacgacagt aggttttatg tgttatttgt
     8521 taattattat tcctttgctc tatcactttc agatagaggc aggactggtt cctataacag
     8581 tacttgtgac acatttgatt gctaaaaaga gtattgcatt acctatttta tcgaatgagt
     8641 ttatgctctt ttttgtaggg actagtgtcg ctttactatt taatgcttat atgggtccac
     8701 aagatcagca aattcggtac tatcaccaaa aagttgaatc ggatctgaaa ggtatcttat
     8761 accgttttga aagttttctg ctggaaggaa aaggtcaaaa tgaagggctc ttgatcaaaa
     8821 accttgataa aatattagat gaagctctaa aactagttta tcgagaaagg cataatcaac
     8881 tatttcagca gaccaattat caagttcatt attttgagat gcgacgacaa caaaatagac
     8941 tattgggaca aatggctatc aacgtaaaca cgttaatgag acaaagtaag gaaagtattc
     9001 ttttgtcaca tctttttcac gaaactgctt gtcagctaag cgaacaaaat ccggctttaa
     9061 ccttgattga tgacatcgaa caattgcttg aaacctttcg tcatggtgat cttcctcaaa
     9121 ctcgggagga atttgagcga cgtgcggttt tatttcagct cttacaagac ttggaacgtt
     9181 ttatcttatt aaaggtagag ttttatcagg attatcaaaa tgactaaatc tctattgcac
     9241 acttcttatc tatgacagct tgggacttaa cttcctgagc ttttttgcta taataaaaac
     9301 aagaatctca gaatggagac aagatgaacg aaataaaatg ccctcattgt cataccctat
     9361 ttactatcaa tgagtcagag tatagtcaat tattggaaca agtcagaggg caagcctttg
     9421 atgaagaatt gcaaaaacgg ctaatcaatg agatagcctt gctagaagaa aaagcgaagc
     9481 atcaattgca tgaggttgtc gcaaaaaaag aaacagctat taccagcttg acaaaccaat
     9541 tagagcaaat tgaaaaagaa caagcctacc tcagacaaga agaactggct aaaaaagacc
     9601 agcttattgc ttctttagaa gcgaaacttg ataaattagc ctcacaaaat gctttggaac
     9661 tggccaatca attagctgag aaggacaaag aagtgattag tttgaccaat cagctggaca
     9721 aattagcact tgaaaaagat gctacgttcc actcaaagct tgctacaatc gaaaaagaac
     9781 gcgatggaat caaaaaccaa ttggtgcttc aagctaaaga aagtgaattg tcgttggctt
     9841 cggtgcgcag cgactacgaa gcacagttaa aagcggctaa cgagcaagtt gaattttata
     9901 aaaatttcaa agcgcagcaa tctaccaaag caatcggaga gagtctagaa ctctatgctg
     9961 aaacagagtt caataaagta agaagctatg cttttcccaa tgctagcttt gtcaaagata
    10021 accaattgtc tagtcgtggg tcaaaagggg actatattta ccgagaggtt gataccaatg
    10081 gtgtagaaat cctctctatc atgtttgaga tgaagaacga ggcagacacc accaaaacaa
    10141 aacataaaaa tagtgatttt ttcaaggagt tagataaaga ccgtcgcgaa aaagattgtg
    10201 agtatgctgt tttggtaagc atgttagaag ctgataatga ttactacaat acagggattg
    10261 ttgatgtcag tcatgagtat caaaagatgt atgtggttcg tcctcaactc tttattcagt
    10321 tgatagggat tctgcgaaat gcagccctga atagtttaca ttataagcaa gagttggcct
    10381 tgattaagga acaaaatatt gacatcactc acttcgaaga ggatttggac caatttaaga
    10441 atgcctttgc taagaactat cagtctgcca gcaacaactt taaaaaggct attgacgaga
    10501 ttgataaatc gattaaacgc atggaggaag tgaaacgctt tttaaccact agtgaaaatc
    10561 agttacgctt agctaataat aaattagagg atgtttcagt taaaaaactg acccgccaaa
    10621 acccaaccat gcgagagaaa tttgaagccc tcaaggacca gtaagcctga aagtgagtct
    10681 ttccctgcta aaattggtta agcgtttatt ttggcaaaac tgatgataaa gcggtagaat
    10741 agaatcagta ataattacta attagagaga aaaatagaca tgataaatgg aatcattaat
    10801 ttaaaaaaag aagccggcat gacctctcat gacgcagttt ttaaattacg gaagttatta
    10861 caagaaaaaa agattggaca tggggggacc ttagatcctg atgtggtcgg tgttctacca
    10921 attgcagttg gtaaggctac tcgtgtgatc gaatacatga cagaagctgg taaagtttat
    10981 gaaggacaag tgactctcgg ttactcaaca acgactgagg atgctagtgg agaggtagtt
    11041 gcgaggtcct ctttgccagc tgtgctaaca gaagaactcg ttgatcaaac gatgacgact
    11101 ttccttggga aaatcacaca gaccccacct atgtattcag ccgtaaaggt taatggccgt
    11161 aaattatacg agtatgcgcg tgctggtgaa agtgtggaac gtcctagaag agaagtgact
    11221 atttctctgt ttgaacgcac cagccctttg aattttactg aagacggtct ttgccgattc
    11281 tcttttaaag ttgcttgtag caaagggact tatgtgcgga ccttagcagt tgatttaggt
    11341 cgagctctag gggtagagag tcatatgtcc ttcttgcagc gatcagcttc agcaggatta
    11401 accctcgaga ctgcctatac tttgggagaa attgctgata tggtttctaa acaagagatg
    11461 tcttttttgt taccgatcga gtatggcgtt gctgatcttc ctaaaatggt tattgatgat
    11521 actgaattga ctgaaatttc atttggtcgc cgtcttagtc tccctagtca agagccactg
    11581 ttagctgctt ttcatggcga aaaagtaatt gctattttag aaaaaagaga tcaagaatac
    11641 aaacccaaaa aagttcttat ttaaatagtt tgagtaagta gaatggataa ttatggaaat
    11701 tgaatatatc aaagattatc gtgatattaa ccaagaagat gatacagtct tgatattggg
    11761 atattttgat ggattacacc gtggtcataa agccttattt gacaaagcaa gagaggttgc
    11821 aaataaagaa gggttaaaag tagtcgtttt tacgtttaca gagtctccga aattggcttt
    11881 ttcgcgtttt tctcctgagt tattgcttca tattacttat ccaaagaagc gttatgaaaa
    11941 atttgccgat tacggtgtaa ataaattata tttggttgat tttacttcta aattctctaa
    12001 ggtctcatct gatcatttta taactcatta tattaaaaac ctgaaagcaa agcatattgt
    12061 tgtcggtttt gactataagt ttgggcataa tcgtacagat agtgattatt taaccagaaa
    12121 ttttgaagga caagtttata ccattgagga aataaaagaa gatcatcgaa aaatttctgc
    12181 tacatggata aggaagctta ttcaagaagg taatgtagtt aaagcaaacc acttgttagg
    12241 ctatgactta tcgacaaggg gaagagtcgt tcacggggat gcgcgtggcc gtactattgg
    12301 tttcccaaca gccaatctag ctccaattga caacacatac ttaccagctg atggagtcta
    12361 tgttacaaat gttattgttg ctaataaaat ctatcgctct atgacgagtt taggtaaaaa
    12421 tgtgacattt ggggggaaag agttgcgtct agaagttaac atttttgact ttgacgaaga
    12481 gatctatgga gaaattattg agattgtttg gttagataag attcgagaca tggaaaaatt
    12541 cgagggaatc gaagatttga ctgatagatt ggaatatgat aaacgaacag cattaaattg
    12601 gaaaaaagat agcaaattat cttaaatttt tgtataataa ggtaagtaaa tttgatagag
    12661 gaaaaatatg gttaccttat ttttatcacc tagctgtacc agttgtcgta aagcaagggc
    12721 atggttagta aaacacgaag ttgactttca agagcataat attattacaa gtccattaag
    12781 ccgtgatgaa ttaatgtcaa tcctctcttt tacagagaat ggaacggagg atattatttc
    12841 aacacgttca aaggtttttc aaaaacttga tgttgatgta gaagagcttt caatttcaga
    12901 tttaattgat ttaattgcta aaaatccaag tcttcttcgt cgccctatta tcatggatca
    12961 aaaacgcatg caaattggtt ttaatgaaga tgaaattaga gcgtttttat cgagagatta
    13021 tcgcaagcaa gaactacgtc aagcaacgat aaaagcagaa attgaggggt gatatgtcag
    13081 gtaattatta ttatccactt gacttaagtt ggagcactga agaaatatcc tcagtgcttc
    13141 attttttgaa taaagtggag ttagcatatg aaaaaaagta gatgcaaaac aactcctaga
    13201 tagttataag acttataaaa ccattgtcaa aagtaaagct caggaaaagc aaatcgaccg
    13261 tgattttcaa aaagttagtg gttattcgac ctatcaagtt gttaaaaaag cgaaggcaat
    13321 tgagaaagga ttcttttccc ttggaaacta aatacgcatt tgcaaggcaa atcattaaag
    13381 aagcagggct ttttatcaag tcaaaaatgt cagagcagct agatattcag gtgaaaacac
    13441 aatttgatga tctggttacc aatgttgatc aagaaactca acagttactg atggatcgta
    13501 ttcatcagac ctatccttgt gatgccattt tagctgaaga aaatgacgtt cgtcacccca
    13561 ttaatcaagg aaatgtttgg gtcatcgatc ccattgatgg aacggttaac tttattgtgc
    13621 agggatcaca gtttgcagtg atgattgcct attatgaaca aggaattggc caatttgggc
    13681 ttatttatga tgtgatggca gatcaattac tagctggtgg tggtgatttt gaagttactt
    13741 taaacggtga taagcttcca gcttaccaag aaaaaccttt agagcgtagc ttaattggct
    13801 gcaatgcagg catgtttgcc agaaatgatc gtaaccttgc tcatctaatt gcaaagactt
    13861 taggtgtaag ggtttatggt ggtgcaggta tctgcatggt taaggtcatg aagcaggaat
    13921 tactggccta tttttcattt atccaaccat gggattacgc agcagcaaaa gtactaggcg
    13981 ataaactggg ttatgtttta ctaactattg acggatatga gcctgatttt caaacacgtc
    14041 aaaaaatcat gtttgttcct aaatgccaac tcactaggat agcgtctttt ctaacaaagg
    14101 actctgccgt cacacttgaa gatcaataga aatgatgtca ttaccaaaag aatttattaa
    14161 cacttatcaa gcaatactag gcaaagaatt agaagatttc ttggcaagtt ttaaccaaga
    14221 gccggtcaat gcttttcgga ttaacccctt aaagaatcaa ctaaaaacct ttgaacaccc
    14281 cattccaaat accttatggg gatattatgg taaactatct ggcaagtctc aagagcatgt
    14341 gtcggggttg gtttattccc aagaaccagc ggctcaaatg gtggcacaag tagcggctcc
    14401 tcaaaagggg agtcgtgttt tagacttggc agctgcacca ggaggaaaat caactcattt
    14461 attagcttat ttagacaata caggtctttt agtcagcaat gaaatttcta agaagcgcag
    14521 caaggtttta gttgaaaata tcgaacgttt tggcgctaga aatgttgtgg tgactaatga
    14581 atccgcagat cgtttagcca aggtattttc tcattatttt gatactatcg tctttgatgg
    14641 accatgttct ggtgaaggaa tgtttcgcaa ggaccctgat gctattcaat attggcatca
    14701 cgggtatccc gcagaatgcg ccaagttgca aaaatctatt ttagaagacg ccttaaccat
    14761 gttaaaacca ggaggagaat tgatatactc cacctgtact tgggctcctg aagaaaatga
    14821 agatgttgtg cagtggctct tagagactta taccttttta gaattggtcg atgttccaaa
    14881 actaaatgga atggttagtg gtatcggact tccagagact gctagaatgt accctcatcg
    14941 ttatcaaggg gaagggcaat ttgtggccaa gctaaaagat aagcgtcaag aaggacaaag
    15001 cactaaacta aaagcaccta aatccaattt gaccaaagac cagttacgtc tttggaaaat
    15061 gtttgaaaaa gaccatctaa accttaccct atcaggaaca ctacaaacct ttggtgatta
    15121 cctatacctc ttgccagatg gtcttcctgc tttagatggc cttaaaattg ctcggaacgg
    15181 tttagaatta ggcgtcttca agaagaaacg ctttgaaccc tcttacgcct tgggattagc
    15241 tttaaagcct gatgaggtcc gatcatctat tgagcttaga gaggaagact ttagacctta
    15301 tgtgtcaggt aacgtcatta ctttgcccca atcttatcca acaggctggt accagttact
    15361 cattaatggc aatggtctag gatttgccaa ggtcgttgac aagaccatca aaaataattt
    15421 ccctaaaggg ttaagatttt agcgtaaaaa ataagattaa aaagttaaat atttaaagaa
    15481 aaaatgcttt caaagcatag gtagatatgt tactataaag aatatgtggc acttagtttc
    15541 tacatatata ccctttattt ttacgaatag gaaataaata atgaaaatga aaaaaaagtt
    15601 ctttttgtta agtcttttgg tcctatcaac tttcttttta tccgcatgtt ctagctggat
    15661 tgataaaggt gagtcaataa ccgctgtagg atcaacagca ctacaaccct tagtagaagc
    15721 agtagctgat gaatttggaa gcagtaatct aggcaagact gtcaatgttc aaggtggtgg
    15781 ttcaggtaca gggttgtctc aagttcaatc aggagctgtc caaattggaa atagtgatgt
    15841 ctttgcggaa gaaaaagatg gtattgatgc ttctaaatta gttgatcatc aagtagctgt
    15901 tgcaggactt gcagttattg ccaatcctaa agtcaaggtt tccaatctca gtagtcagca
    15961 gttgcaaaag attttttcag gagaatatac caattggaaa caagttggag gagaagatct
    16021 tgcgatttca gtgatcaacc gagcagcaag ttctggctca cgagcaacct ttgacagtgt
    16081 tatcatgaaa ggggtcaacg ctaaacaaag tcaagagcaa gactccaatg ggatggttaa
    16141 atcgattgtt tcacaaacac caggtgccat ttcttacctt tcctttgcct acgttgattc
    16201 atctgttaaa tctttgcaat taaatgggtt taaggcaaat gctaagaacg tggctacaaa
    16261 tgattggcca atctggtcct acgaacacat gtataccaaa gataaaccaa cagggttgac
    16321 caaggaattt cttgattata tgttttcaga tgaagtacaa cagaacattg ttacacatat
    16381 gggatatatt tcgataaatg atatggaagt ggtcaaatct catgatggaa aagtaacaaa
    16441 aaggtaatgg aggaattatg gaaaatcagg aattggctaa aaaattagcc tcaccatcaa
    16501 agaattcaag gcttgaaacc tttggccgca ccattacgtt tttgtgtctg gctttgattg
    16561 tttttatcgt tgcgatgatt ctgatcttcg ttgctcaaaa agggttgtca accttctttg
    16621 ttgacaaggt taatcttttt gatttcttgc ttggaaagga gtggcaacca agtgtgaaaa
    16681 atgcggccgg cattccttat cttggtgctc tgccaatgat tacaggatcc tttttggtta
    16741 ccattttatc tgctattatt gccaccccat ttgccattgg cgcagccgtt tttatgactg
    16801 aaatctcacc taaatatggc gctaaattat tacagcctgc ggttgagctt ttggttggaa
    16861 ttccttcggt tgtttatggg tttatcggtt tgcaagtgat tgttcctttt atgcgctcta
    16921 tctttggtgg cacaggtttt ggaatcctat ctggggtctg tgtcttgttt gttatgattt
    16981 taccaacagt gacttttatg acaacagaca gtctgcgggc ggtgcctcgt cattaccgcg
    17041 aagcgtctat ggctatggga gcaacacgtt ggcaaaccat ctggcgtgtt gtccttaatg
    17101 cagctcgtcc aggaattttt accgctgtta tttttggaat ggcgagagct tttggagaag
    17161 ccttggctat ccaaatggta gtcggtaact ctgctgtaat gccaagttca ctaacgacac
    17221 cagccgcaac cttaacgtct gtcttgacaa tgggtattgg taacacggtt atgggaacgg
    17281 tccaaaataa tgtgctttgg tcccttgccc tagtcttatt attaatgagt ctggccttca
    17341 attcccttgt caaattaatc acgaaagaaa gaaagagaaa ttatgaacgc taaaaaagtc
    17401 gataaagtag caactggtac tctttatacc attgctggaa ttattgtagc tattttagct
    17461 tccttaattc tatatatcct tgtccgtggc ttgccacaca tcagctggtc ctttttaaca
    17521 ggaaaatcgt cttcgtacga agctggtggg ggaattggta tccagttgta taattccttc
    17581 ttcctattga ttgttacttt aattatttcc attcctttat caactggagc ggggatttac
    17641 ttggctgaat atgccaaaaa agggcctgtt accaacttta ttagaacctg tattgagatt
    17701 ctgtcttccc taccatctgt ggttgtgggg ctctttggtt acttgatttt cgttgtgcag
    17761 tttgaatatg gcttttctat tatttcaggg gctcttgctt tgacggtctt taatcttcct
    17821 caaatgaccc gtaatgttga agatagttta ctgcatgttc atcataccca aagagaagca
    17881 ggattagctt taggcctgtc acgctgggaa acggtttttt atgtggttat cccagaagct
    17941 ctcccgggaa tggtaacagg tattgtctta gcttcaggtc gtatttttgg tgaagcggca
    18001 gcgcttatct atactgctgg tcaatcagca ccagctctgg attggtcaaa ttggaatcca
    18061 cttagtgtta ctagtccaat ctctattttc cgtcaatctg agacccttgc tgttcacatt
    18121 tggaaagtca atagcgaagg gacaattcca gatgctactc ttgtatccgc aggaagtgca
    18181 gcagtgctat taatttttat ccttattttc aacttttcag ctcgctttat tgggaagaaa
    18241 cttcattcta aaatgacagc ggccaaataa aatagtggag aaatcatgac agaatacaat
    18301 tggaatgaac gtcatatcat cacttttcct gaagaaacgc ttgctctagc taccaaggat
    18361 ttacatgttt actatggtgc gaaagaagct atcaaaggga ttgatatgca gtttgaaaaa
    18421 cataaaataa cagccttgat tggtccatct ggttgtggca aatcaactta tcttcgtagt
    18481 cttaaccgca tgaacgatac cattgatatt gctcgggtta caggtgagat tctttatcag
    18541 ggcattgatg ttaatcgaaa agacatgaat gtttacgaga ttcgcaagca cttaggaatg
    18601 gtttttcaaa gacctaatcc ttttgctaaa tccatttata aaaacattac ctttgcccat
    18661 gaaagagctg gtgtgaaaga caaaaaagtt ttagatgaaa ttgttgagac ttcactgaaa
    18721 caagccgcgc tttgggatca agttaaagat gaccttcaca aatcagcctt caccttatca
    18781 gggggtcagc aacaacgctt gtgtattgct agagccattt ctgtcaaacc tgatattcta
    18841 ttaatggatg aaccagcctc tgcccttgac ccgattgcaa cgatgcagct ggaagaaacc
    18901 atgtttgaat tgaagaagaa ttacacgatt attattgtga cccacaatat gcaacaagca
    18961 gctcgtgcca gtgattacac tgccttcttc tatttaggca acctcattga atatgataag
    19021 acacgtaata ttttccaaaa tgcgcaatgt cagtcaacaa acgactacgt ttcagggcac
    19081 tttggttaga aaggcaatat gatgacagaa tctattttac agatcagaga cctttctgtt
    19141 tactacaatc aaaagaaaac gttaaaagat gtctctttgg acctttatcc aaatgaaata
    19201 acagctttaa ttggtccttc tggatctggg aaatcaacgc tcttgcgttc tatcaaccgt
    19261 atgaatgatt tgaatcctga agtaaccatt acaggctcta tcgtctataa cggacacaat
    19321 atttatagtc caaggacaga tacggttgat ttacgcaaag aaattggtat ggtattccaa
    19381 caacccaacc catttccgat gtctatttat gagaacgtgg tttacggcct tcgcttaaaa
    19441 ggtatcagag ataaatcaat cttagaccat gctgttgaaa gctctcttaa aggggcttcc
    19501 atttggaatg aagtcaaaga tcgtttacat gattctgcgg ttggcttatc aggcggacag
    19561 caacagagag tttgcatagc tagagttctt gctaccagcc ccagaataat ccttttagat
    19621 gaacctacct cagctcttga tcctatctca gctgggaaaa ttgaagaaac attattgtta
    19681 ttaaaaaaag attatacact ggctattgtg acccgctcaa tgcagcaagc ctcacgttta
    19741 tcagatcgaa caggtttttt cttggagggt gacttacttg agtgtggtcc tacaaaagcc
    19801 atgtttatga atcctaagcg caaagaaacc gaagactata tttctggtaa atttggttga
    19861 gaaagacgta tatggttgac ccctctgtgt ttttattata atgactagat tgtaggaaga
    19921 aaaaatcatg ttaagaacga aatttgaaga agaattagat aaattgcata accagtttta
    19981 ttcaatggga acggaagtat tggcacaaat caataaaact gttcgtgcct ttgttagtca
    20041 tgatcgtgaa ttggccaaag aagttatcga agaagatgac actattaata attttgagac
    20101 aaagcttgaa aagaaatcgc tggaaattat tgccctacaa cagccagttt cgaatgatct
    20161 tagaatggtt attactgttt taaaggcatc aagtgatatt gaacgtatgg gtgaccatgc
    20221 tgcttcgatt gccaaggcca ctattcgtat gaaaggtgag gagcgtattc cagtggttga
    20281 agaacaaatc aaccttatgg gaaaagctgt gaaacagatg gtagaagaag cccttaatgc
    20341 atatatcaat gctgacgata caaaagctta tgaaatcgca gcttctgatg agattatcga
    20401 tcaatatttc cgtaatatcc aaaccttagc tgttgaagaa atccgtaagt ctccagatgc
    20461 tgtttttgca ggaaaagaat attttcaagt attaatgtat ttggaacgta ttggtgacta
    20521 tgctcgcaac atttgtgaat ggattgttta tcttaaaact ggaaaaatta ttgaattata
    20581 agaaattatg ctataatgag actgggaatc agcttgccag tccctggttt tatacgaaat
    20641 tgtgcaagtt agaaaatgaa ctaacaagaa aagttttctt tgctgaatct gattttgtta
    20701 ggttaacaaa tatatgtaga cagagatgat acttaggtat catctgttga tctattatga
    20761 tttgttatta gaattaagga gttatatgaa gacagtagaa catctcatcg aaacatttgt
    20821 gcctgaaaat tataacatct ttttagatat taatcgtcag actaaaacat ttacggggaa
    20881 tgttgcgatt aatggtgaag ctttagataa tcatgtggct ttccaccaaa aagatttgga
    20941 cattaagtct attttgctcg ataatgaagc tgttatttat caagtggata acgacaatga
    21001 agttgtgcgt gttgaattac ctgaaacagg aatgatgacg cttgtcattg aattttcagg
    21061 tagcattaca gataatatga caggtattta cccttcatat tacactaaaa atggtgagaa
    21121 aaaagaagtc atttcaactc agtttgaaag ccattttgcg cgtgaagctt tcccatgtat
    21181 cgatgaacca caagcaaaag ctacttttga tttaagcttg acctttgatc aagaaattgg
    21241 tgagatagcg ttatcgaata tgccagaagt caatattgac cgtcgcgagg agactggtct
    21301 ttggacattt gatacgacac caagaatgtc atcttacttg ctagcgtttg cgctaggaga
    21361 acttcatgga aagactgtag aaagcaaaaa aggaacaact gttggtgtgt atgcgacaac
    21421 agcgcatcct cttagctcgc ttgatttttc attggatatt gcagttcgtg ttatcaactt
    21481 ttatgaagac tattttggag tacattaccc aatcccgcaa tctcttaata ttgcgcttcc
    21541 agatttttca tctggtgcta tggaaaattg gggtttaatc acttatcgtg aaatttattt
    21601 attagtagat gaaaattcga ctgttcaaag ccgacaacaa gttgcacttg taatcgccca
    21661 cgagatagca caccaatggt ttggaaactt ggttacaatg aaatggtggg acgatctgtg
    21721 gttaaatgag agttttgcca acatgatgga atatgtctct atcgaggcta ttgagccgtc
    21781 ttggaaaata atagaagatt tccaaacagg tggtattcct cttgctttaa aacgtgatgc
    21841 tactgatggt gttcaatcag tgcatgttga agtcaatcat ccagatgaga taaatacatt
    21901 gtttgatcct gctattgtct atgcaaaagg tagtcgtttg atgcatatgt tacgccgttt
    21961 tattggtgat cgtgattttg ccattggctt acatcactac tttgaaaaat atcaatatcg
    22021 aaatactgtc gggcgtgatt tatggaatat tttatcagat acttcaggca aagacgtagc
    22081 tgcttttatg gatgcttggc ttgaacaacc aggttatcca gttttaacag ctcgccttga
    22141 aaacgatcaa ttgattttga gtcaaaaaca attttttatt ggaaaaggtg aagaaaaagg
    22201 tcgcctttgg ccgattcctt taaacactaa ttggcatggc cttcctgaaa ctttgacaga
    22261 ggcagaaatg gtcattccaa actttagtca attggcagca gaaaatgaag gagctctacg
    22321 tttcaatatt ggtaatacag ctcattacat tacagactac caaggatcat tattagatgc
    22381 cttagtcact gaacttgccc aacttgataa tacttctgct ttacaagtta ttcaagaacg
    22441 tcgtttattg gcagatagtg gcttgatttc ttatgctgaa ttggttgact taattgctca
    22501 attggacgat tctaagtctt atatggtagc agaagcggtt cagcaagtgg tctctggttt
    22561 gaaacgcttc attgatgaag gaagcttagc agaaaaatcc tttaatcgct tggtaaccac
    22621 catctatcaa gaagacttta atcaccatgg ttttgagaaa aaagctgacg aatcagatga
    22681 agatgagatg gtacgtcagg ttgccttggg acgcttgtgg ttagcagaaa atcagacgat
    22741 tattgacgga ttgagaacta tttttgaagc atatcaaagc aatattgcaa gtattccagc
    22801 agctgttcgt cgcttggttt tagccaacca aatgaaatat tttgaaacag atagcttggt
    22861 tgatatttat tttgagactt atgtagcaac tactgataat aatttacgca gtgatttaac
    22921 ggttgctttt agtcaaacaa aacaatcaac tactattaga cgtattcttg ttagtttaaa
    22981 agataaagat attattaaac ctcaggattt atcttattgg tacaatgctt tgcttggaca
    23041 aagctttact caagatatca tttgggaatg ggctagagaa aattgggatt ggattaaatc
    23101 tgcacttggt ggagatatga gctttgataa atttgttatt tatccagcaa gtaactttaa
    23161 aacaccaaaa catctagcag aatataaatc tttctttgaa cctaagctag atgatatggc
    23221 tattagccgt aatattacca tgggaattaa tgaaattgaa gcacgtgttg ctttgatcac
    23281 taaagaaaaa gaagcagtta ttgcagctct cagtcattac tagaccataa gcaaaagagt
    23341 tgggggactt tccagctctt ttttgtagta agctagcttc ctttcttagt tccgtctata
    23401 actaaatgat tatccatagt ttaattcaaa aggagattgc actttaaagc gaaggctatt
    23461 agcctcttaa atactttttt accaggtggt gaataagaac aattataaat gatttaatca
    23521 ttatgcttta aatgccttaa aggagtatta atattttagt aaaccttaag ctttttgtag
    23581 tatagtctcg ttagaaattg ttatcagaaa tgagactaag ttaaatgaag tgatagttac
    23641 tttatttaag aacaattgag gtagagacat gataaaaata ttattagtag aagatgactt
    23701 gagcttatca aattccattt ttgatttttt agatgatttt gcagacgtca tgcaagtttt
    23761 tgatggcgat gaaggtttat acgaagcaga aagtggtatt tatgatttga ttttgttaga
    23821 ccttatgttg ccggagaaaa atggctttca agtcttaaaa gaattgcgag aaaaagatat
    23881 taaaattcct gtgttgatca tgacagctaa agagggttta gatgataaag gtcacggatt
    23941 tgaattaggt gctgatgatt atctgaccaa gccgttctat ttagaagagt taaaaatgcg
    24001 aattcaagca ctgttaaaaa gaacagggaa atttgcagat aaaaatatta gttttggcaa
    24061 tttagtggtt gacttagctc gaaaagaggt gaaggttgaa gggaaagtag ttgaattact
    24121 tggcaaagaa tttgatttgt tggtgtatct tttgcaaaat caaaacgtta ttttaccaaa
    24181 gacacaaatt tttgatcgtt tatggggatt cgatagtgat actacgattt cggttgttga
    24241 agtttacatt tctaaaataa gaaaaaaatt aaaaggtacc cgttttgtta acaggcttca
    24301 aactttaaga agcgtagggt atattctaaa aaacaatgaa taaattaaaa aaagagattt
    24361 tatcagataa ctataaccac ttttttcatt tttttgcggt ttttacaggt atttttgtca
    24421 ttatgactat tattatctta cagattatgc ggtttggcgt ttattcgtca gttgacagta
    24481 gtttagtttc tgttagtaat aatgcaagta gctatgctaa tcgtacgatg gctagaatat
    24541 cttcttttta ctttgatact gaaaataaca ttattaaggc gctgcctgat tcagatagtt
    24601 ctaagttatt aggaacgcct gcagctaata cagatatcat tttgtttagt gctaatggaa
    24661 caattttaaa tgcttttgat gcgttttcta actatcaaaa ttttcattta gataaatacc
    24721 gtttggggag tattgaaacc accagtttaa tgaattttta tggacaagaa gaaaaatacc
    24781 atacgataac tgtaagggtt catatcaaaa attatcctgc agttgcctat atgatggcag
    24841 tagtaaatgt ggaacaatta gaccgcgcta atgaacgtta cgagcgcatt attattatag
    24901 ttatgagtgt tttttggcta atttctattt tagcaagtat ttatttagcc aagtggagca
    24961 gaaaacctat tttagaaagc tatgaaaaac aaaaaatgtt tgttgaaaat gctagtcatg
    25021 aactaaggac ccctttggcg gtcttacaga atcgtctgga atcgcttttt cgtaagccca
    25081 acgaaacgat attagaaaat agtgagcatc tcgcttctag tttagacgag gttcgcaaca
    25141 tgcgcatctt aacaactaat ttattaaatt tagcaagacg agatgatggc attaatccac
    25201 agtggactca tttagataca gattttttta atgctatttt tgagaat
//
LOCUS       DAUDYX010000026        24992 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.026, whole genome shotgun sequence.
ACCESSION   DAUDYX010000026 DAUDYX010000000
VERSION     DAUDYX010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24992
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.026"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..206
                     /locus_tag="VHD02_001047"
     CDS             <1..206
                     /locus_tag="VHD02_001047"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ5497664.1"
                     /translation="TLKIHPNSLYRWIQEYEKYGESAFPGHGSALRHAQFETKKLEKE
                     HKLLQEELALLKKFQVFLKPNRK"
     gene            155..808
                     /locus_tag="VHD02_001048"
     CDS             155..808
                     /locus_tag="VHD02_001048"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEQ5497665.1"
                     /translation="MSSSKKVPGLFEAKPEIKFQFLKENSVSLNSHHACQTLQVSRSG
                     FYAYLKRRPSSRHVENEALKEMIKAIFYEHKERYGSVRITQELCRRGIHVNYKRVGRL
                     LHQLGLYAKGSRYQYKYYNRRRSSLTRPNLVNQCFQATGKNKLWLGDLTYIPTQEGIL
                     YVSVFIDVYSRKIVGWAMGRRMQDKLVTEAFNQAYNREKPKEGVIVHTDQGSQYTGA"
     gene            848..1063
                     /locus_tag="VHD02_001049"
     CDS             848..1063
                     /locus_tag="VHD02_001049"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ5497666.1"
                     /translation="MSRKGNPYDNALMESFYKTLKRELVNDAHFATIEQAQLEIFKYS
                     ETYYNPKRLHSALGYLSPVEFEKIVTH"
     gene            1190..1345
                     /locus_tag="VHD02_001050"
                     /pseudo
     CDS             1190..1345
                     /locus_tag="VHD02_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1392..1922)
                     /locus_tag="VHD02_001051"
     CDS             complement(1392..1922)
                     /locus_tag="VHD02_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HEQ5497667.1"
                     /translation="MSNFISPVYEIKSIPIEKISPNDYNPNSVAPPEMKLLYDSIKSD
                     GYTMPIVCYYDKEEDRYSIVDGFHRYRIMLDYSDIYERESGRLPVSVIDKSLDYRMAS
                     TIRHNRARGSHDVDLMSQIVKDLHECGRSDNWIAKHLGMDKDEILRLKQITGLASLFK
                     DHEFNQSWEPEELLNE"
     gene            complement(1915..3249)
                     /locus_tag="VHD02_001052"
     CDS             complement(1915..3249)
                     /locus_tag="VHD02_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HEQ5497668.1"
                     /translation="MKKYLNQNVYDALIERLHFLFNEFPIVYISFSGGKDSGLLLNIL
                     LDFRDKYYPDREIGVFHQDFEAQYSLTTKYVQETFTSLEGRKKVSLYWVCLPMATRTA
                     LSSYEMFWYPWDDKTEDIWVRPMPSQDYVINLENNSITTYRYKMNQEDLAKQFGRWYK
                     QIHGNQKTVCILGNRASESLHRYSGFINKKYGYQKECWITKQFKDVWTASPLYDWSVE
                     DIWHAYYKFSYSYNELYDLFYKAGLKPSQMRVASPFQDYAVDSLNLYRIIDQETWVKL
                     LGRVQGVNFSNIYGRTKAMGYKSIALPKGHSWKSYTQFLLSTLPVRLRNNYVRKFNKS
                     IDFWHKTGGGLAEETINELIEKGYRIARNGISNYTSFKHSRVIFLDQIPDDTDDIVTT
                     KDIPSWKRMCFCILKNDHICRTMGFGLTREQQKIVAFLKEKYKTSLKELESE"
     gene            complement(3471..3770)
                     /locus_tag="VHD02_001053"
     CDS             complement(3471..3770)
                     /locus_tag="VHD02_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497669.1"
                     /translation="MFLTWENLFLTNQSRRRLKLLVVERSYNNVGNFLKEYFGGFFEI
                     INFDDLRLTMVDMVSLSSEYDVIVTDMILEQTMDSEILFFNQMAPSVVANRLTDM"
     gene            complement(3927..4394)
                     /gene="perR"
                     /locus_tag="VHD02_001054"
     CDS             complement(3927..4394)
                     /gene="perR"
                     /locus_tag="VHD02_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEQ5497670.1"
                     /translation="MDIHSHQQALDAYENVLEHLREKHIRITETRKAIISYMIQSTEH
                     PSADKIYRDLQPNFPNMSLATVYNNLKVLVDEGFVSELKISNDLTTYYDFMGHQHVNV
                     VCEICGKIADFMDVDVMDIAKEAHEQTGYKVTRIPVIAYGICPDCQAKDQSDF"
     gene            complement(4446..4901)
                     /locus_tag="VHD02_001055"
     CDS             complement(4446..4901)
                     /locus_tag="VHD02_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEQ5497671.1"
                     /translation="MIYSFQNPSEDILKAYLESAKTIAVVGLSDRKDTAAYGVAKFMQ
                     AMDYRIIPVNPKLAGQLILGEKVYASIKAIPFEVDIVDVFRRSEFLPEVARDFLAGQA
                     KVFWAQLGLENQEVQTILRSAGKEAIVMNRCLKIDYLQLVAKPECITNL"
     gene            complement(5088..7730)
                     /gene="polA"
                     /locus_tag="VHD02_001056"
     CDS             complement(5088..7730)
                     /gene="polA"
                     /locus_tag="VHD02_001056"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEQ5497672.1"
                     /translation="MENKNKLLLIDGSSVAFRAFFALYNQIDRFKNHSGLHTNAIYGF
                     HLMLDHMMKRVRPTHVLVAFDAGKTTFRTEMYADYKAGRAKTPDEFREQFSYIREMLT
                     ALGIAYYELEHYEADDIIGTLDKMAERTEVPFDVTIVSGDKDLIQLTDENTVVEISKK
                     GVAEFEEFTPAYLMEKMGLTPNQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLHEFGS
                     LEGIYEHIDGFKTSKMKENLINDRDQAFLSKTLATINTASPITIGLDDIVYNGPDVAS
                     LSQFYDEMDFVQLKKGLASQMPQEPVAVISYQEVTNVSADLFSAEDIFYFETLRDNYH
                     REAIIGFAWGHGEQIYASTDLSLLATDSFKQVFQKPIATYDFKRSKVLLSHLGIELVA
                     PSYDARLANYLLSTVEDNELSTIARIFTDISLEEDDTVYGKGAKRAVPDKDVLLEHLA
                     RKVKVLLDSKSQMLDKLTAHEQLDLYQNIELPLANVLAKMEIEGIKVNRATLQDMAEQ
                     NKVIIEALTQEIYDMAGQEFNINSPKQLGSILFEKMQLPLEMTKKTKTGYSTAVDVLE
                     RLAPIAPIVAKILDYRQITKLQSTYVIGLQDYILADGKIHTRYVQDLTQTGRLSSVDP
                     NLQNIPIRLEQGRLIRKAFTPSHEDAVLLSSDYSQIELRVLAHISGDEHLIAAFNEGA
                     DIHTSTAMRVFGIDKAADVTANDRRNAKAVNFGIVYGISDFGLSNNLGITRKQSKSYI
                     DTYFERYPGIKAYMENVVREAKDKGYVETLFKRRRELPDINSRNFNVRSFAERTAINS
                     PIQGSAADILKIAMINLDKALQAGGFRAKMLLQVHDEIVLEVPNDELTAIKKLVKDTM
                     EAAVDLAVPLCVDESTGQSWYEAK"
     gene            complement(7861..9588)
                     /locus_tag="VHD02_001057"
     CDS             complement(7861..9588)
                     /locus_tag="VHD02_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEQ5497673.1"
                     /translation="METILQTKLPVAQLVEQLTEWLTKTFSGLFDIMQVVGSFLMDWM
                     TKTLLFIHPLLFIVLVTAGMFFLAKKKWPLPTFTLLGLLFIYNQGLWKQLMNTFTLVL
                     VASLISVLIGIPLGIWMAKNATVRQIVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLAIRDIPTELIEASDAFGSTGKQKLFKVELPLAKNTIMAGVN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGSGFVSGLALVILAIVLDRMTQLF
                     NSKPQEKAKAGKTNKWIGLAALAVFLIAALGRGIMTMASGMADKGETVNIAYVQWDSE
                     VASTHVIAEVLKNEGYHVTLTPLDNAVMWQTVANGNADFSTSAWLPVTHGQQYQKYKS
                     KLDDLGPNLKGTKLGLAVPKYMTDVNSIEDLSKQADQKITGIEPGAGIMAAAKKTLKE
                     YHNLSSWELVAASTGAMTTSLDQAIKKKDPIVVTAWSPHWMFAKYDLKYLKDPKETFG
                     STENINTIARKGLKKDLPNVYKIIDKFHWTQKDMEAVMLDINKGMSPEAAAKKWVEAN
                     KSKVSSWTK"
     gene            complement(9604..10800)
                     /locus_tag="VHD02_001058"
     CDS             complement(9604..10800)
                     /locus_tag="VHD02_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEQ5497674.1"
                     /translation="METILEVKHLSKIFGKKQKAALEMVKTGKNKSEIFKKTGATVGV
                     YDASFEVKKGEIFVIMGLSGSGKSTLVRMLNRLIEPSAGSILLEGKDISTMSADQLRE
                     VRRHDINMVFQSFALFPHKTILENTEFGLELRGVPKEERQRLAEKALDNSGLLDFKDQ
                     YPNQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDSMKQTI
                     IFISHDLNEALRIGDRIALMKDGQIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTTVELDGPQVALNRMHNEEVSMLMATNRRRQLVGSLTADAAIEARKKGL
                     PLSEVIDRDVRTVSKDTIITDILPLIYDSSAPIAVTDDNNRLLGVIIRGRVIEALANI
                     SDEDLN"
     gene            complement(11288..12379)
                     /gene="ulaG"
                     /locus_tag="VHD02_001059"
     CDS             complement(11288..12379)
                     /gene="ulaG"
                     /locus_tag="VHD02_001059"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEQ5497675.1"
                     /translation="MTKVQDITRESWILNTFPEWGTWLNEEIEQEVVPADNFAMWWLG
                     NCGIWIKTPGGANVVMDLWSNRGKATKQVKDMVRGHQMANMAGARKLQPNLRAQPMVI
                     DPFMINELDYYLVSHYHSDHIDINTAAAIINNPKLNHVKFVGPYECGEVWKNWGVPKD
                     RIMILKPGDSFEFKDIKITAVESFDRTCLVTLPIQGADAQDGDLAGLAITDDDMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGRDYDIDVVLNNYGENPIGIQDKMTSVDLLR
                     MAENLRAKVVIPVHYDIWSNFMASTDEILELWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DQNRIEYHHPRGFDDCFLEDSNIQFKALL"
     gene            complement(12632..14278)
                     /locus_tag="VHD02_001060"
     CDS             complement(12632..14278)
                     /locus_tag="VHD02_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEQ5497676.1"
                     /translation="MMILDKKSYDLLSYLLKLETPETVMAISHALNQSRRKVYYQLDK
                     INQALPKGVDQIISYPRLGILLTADQKAACRLLLEEVTDYNYVMKSDERRRLSSIYIA
                     VSTERVTIDKLMQINDVSRNTILNDLTELREELEDKQYKIQLHATKARGYYFGCHPMA
                     LIQYLYKLLVDVYQGGNTSFIDIFNRKLSEIQGLSVYFSKDILTYFHEYLFLSQASLG
                     KTINTQDSQFMLQILPFMLLSYRNMRLDSETKSALKQEFHLIWKRKEYHIAQDLAREL
                     YHNFKLHLDDIEVSMVAMLMLSFRKDQDHHVESQDYDDMRATISHFIDQLESRYQLHF
                     THKQDLLKQLTTHCKALVYRKAYGIFLVNPLTDHIKEKYEELFAMTQSCATILEQDWT
                     ISLTDDDIAYLTIHLGGELRHNNTEQEKTKLVIVSDDGIGIQKLLFKQCQRYLANGQI
                     EAVFTTEQYQSVYDLLAVDMIVATTDTLKTKIPMLIVNPILSDDDIIKLIRFSKQGRL
                     SEHSRFSTELTKAIEAVVKDESDRYALVSKIEKLIHRELL"
     gene            complement(14603..15307)
                     /locus_tag="VHD02_001061"
     CDS             complement(14603..15307)
                     /locus_tag="VHD02_001061"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEQ5497677.1"
                     /translation="MAKNLQEMRERVCAANKSLPQHGLVKFTWGNVSEVCRELGRIVI
                     KPSGVDYDLLTPENMVVTDLDGNVVEGDLNPSSDLPTHVELYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLTKAEVDGAYEQETGNVILEEFSKRGL
                     DPMAVPGIVVRNHGPFTWGKTPEQAVYHSVVLEEVARMNRLTEQINPRVEPAPRYIMD
                     KHYLRKHGPNAYYGQK"
     gene            complement(15309..16172)
                     /locus_tag="VHD02_001062"
     CDS             complement(15309..16172)
                     /locus_tag="VHD02_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEQ5497678.1"
                     /translation="MARPIGIYEKATPKQFTWRERLQFAKDLGFDFVEMSVDESDARL
                     ARLEWTKEERLDLVKAIYETGIRIPTICFSGHRRYPLGSNDPAIEAKSLKLMKQCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPETRARFIKNLRQSCDWAEEAQVMLSIEIMDDPFIN
                     SIEKYLAVEKEIDSPYLFVYPDAGNVSAWHNDLWSEFYNGHKSIAALHLKDTYAVTET
                     SKGQFRDVPFGQGCVDWQELFAVLKKTNYNGPFLIEMWSENCDTVEETKAAIKEAQDF
                     LYPLIEKAGLA"
     gene            complement(16177..16839)
                     /locus_tag="VHD02_001063"
     CDS             complement(16177..16839)
                     /locus_tag="VHD02_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEQ5497679.1"
                     /translation="MTHIPNLQVALDHSDLQGAVKAAVAVGHEVDVIEAGTVCLLQVG
                     SELVEVLRSLFPEKIIVADTKCADAGGTVAKNNAKRGADWMTCICCATIPTMEAALKA
                     MKEERGDRGEIQIELYGDWTYEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNKV
                     KTLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAEDPAAAARAFKDEIKRI
                     WG"
     gene            complement(16930..17415)
                     /locus_tag="VHD02_001064"
     CDS             complement(16930..17415)
                     /locus_tag="VHD02_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ5497680.1"
                     /translation="MNLKQAFIDNNSIRLGLSADTWQEAVQLAVQPLIDSKAVTSAYY
                     DAIIASTEKYGPYYVLMPGMAMPHAEAGLGVNRNAFALITLTKPVTFSDGKEVSVLLT
                     LAATDPSIHTTVAIPQIVALFELDNAIERLVACQSPKEVLEMVEESKDSPYLEGMDLN
                     A"
     gene            complement(17538..17816)
                     /locus_tag="VHD02_001065"
     CDS             complement(17538..17816)
                     /locus_tag="VHD02_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ5497681.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDIQSASCSVGEA
                     KGLASGYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEVL"
     gene            complement(17887..19320)
                     /locus_tag="VHD02_001066"
     CDS             complement(17887..19320)
                     /locus_tag="VHD02_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEQ5497682.1"
                     /translation="MEMLLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPIYEVFAG
                     FVKATVGYLILNVGAGGLVTTFRPILVALAKKFELKAAVIDPYFGLAAANTKLEEMGF
                     ISVATTALLIGFGVNILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQFQNA
                     FGAWAVGIICGLYWAISSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGAILAVLGPDIMSDVDLIGPGAFNPAKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVSELTNAFQGISSKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVIFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGILQVALGAVAVGLLGLTGGYHGNIDLVLPWLPFGYLFKFLGIAGYVLV
                     CIFLLAIPQLQFAKAKDKEAYYRGEAQ"
     gene            complement(19627..22128)
                     /locus_tag="VHD02_001067"
     CDS             complement(19627..22128)
                     /locus_tag="VHD02_001067"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEQ5497683.1"
                     /translation="MTFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTSDQ
                     AEAVADYKRQASLKSDLARTDLAKEKTGVWTGSYAINPVNGKEMPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFNLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKASA
                     IAKMVEWLEAEGVGNENVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANVTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQALTASGESISYVPWPSYDESKLVENDVEIVVQIKGKVKAKLVVAKDLSREEL
                     QEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVIK"
     gene            complement(22339..23478)
                     /locus_tag="VHD02_001068"
     CDS             complement(22339..23478)
                     /locus_tag="VHD02_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEQ5497684.1"
                     /translation="MRENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKHL
                     YTRFSNPTTEALEDGLACLEKATYAVAYASGMAAISTVLMLLKAGDHVIFPLEVYGGT
                     CQFATAILPNYQIETSFVDMADLATVKASIRPNTRMIYLETPSNPLLKICDISELAQL
                     AKAYGVLTVADNTFMTSLYQEPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYN
                     QLKLFQKNFGAIVGVEDAWLILRGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYP
                     GLDSHPNHDTHLQQAKNGGAVLSFELASKEELMSFTHRIQLPILAVSLGGVESILSHP
                     ATMSHACLSSQARLEQGVVDGLLRLSCGVENIEDLLADFEQALAF"
     gene            24434..24601
                     /locus_tag="VHD02_001069"
     CDS             24434..24601
                     /locus_tag="VHD02_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497685.1"
                     /translation="MKKKLSLFMIATAAVAGLSLATLGSHQTVSANDASYSNGFYGHT
                     GPDSWLLYTVW"
BASE COUNT         7528 a         5407 c         4616 g         7441 t
ORIGIN      
        1 caactttaaa aattcatccc aatagtcttt atcgatggat tcaagaatat gaaaaatatg
       61 gagaaagtgc gttcccagga catgggagcg cacttcgtca tgctcaattt gagacaaaaa
      121 aacttgaaaa agagcacaaa ctgctacagg aggaattagc tcttctaaaa aagttccagg
      181 tctttttgaa gccaaaccgg aaataaaatt tcagtttctg aaggaaaata gtgttagctt
      241 gaatagtcat cacgcttgtc aaactcttca agtctcccgt tcaggtttct atgcttattt
      301 aaaacgtcgt ccctcttcaa ggcatgttga gaatgaagct ttgaaggaaa tgattaaagc
      361 cattttctat gaacataaag agcgttacgg aagtgtacga attacccaag aactttgtag
      421 acgtggcatc catgtcaatt ataaacgtgt tggtagactc cttcatcagc taggtctcta
      481 tgctaaagga agtcgatatc aatataaata ctataaccgt cgacgttctt cattaactcg
      541 tcccaatctt gttaatcaat gtttccaagc gactggtaag aataaattat ggctaggaga
      601 cctaacctac attcctacac aagaaggaat actttatgtg tctgtcttta tagacgttta
      661 tagtagaaag attgttggtt gggcaatggg ccgacgtatg caagacaagt tggtaacaga
      721 agctttcaat caagcttata atagagaaaa gcctaaagaa ggggtaatcg tccatacaga
      781 tcagggttct caatataccg gagcatgatt tcaggaccta cttagacaga aaaaatgtaa
      841 gtccaggatg agtcgaaaag gtaatccata cgataatgca ctcatggaat ccttttataa
      901 aacattaaaa agagagcttg tcaatgatgc tcatttcgca actattgagc aagctcagct
      961 tgagattttt aaatatagtg agacttacta taaccctaaa cgtctgcatt cagctttagg
     1021 gtatctctca cctgttgaat ttgaaaaaat agttactcat tagcttaact ttatgtctat
     1081 tttttcttga caagtccagg gtatgaagtc cctttataca catactttca aaacagctct
     1141 caatcctctg tccgctacaa aaatacgtca ccgctgccct ccagctgcct cattcaacga
     1201 caaacttgaa gccacaaaca gactcatcaa gggcattaag tacaatgcct ttagatttca
     1261 gaactttgac aactttaaaa ccaaaatcct catcgctttg aacatccaaa aagagagaac
     1321 caatctgatt ctctctcgtt gttaatgata agtcacccac tacagttgac aatgagtctc
     1381 ttacttcctg tttactcatt taatagctct tctggttccc aagattgatt aaactcatgg
     1441 tccttaaaaa gagaagccaa tcctgttatt tgtttcagtc ttaaaatttc atctttatcc
     1501 attcctaaat gttttgcaat ccagttatca cttcgcccac actcatgaag atcctttaca
     1561 atctgactca tcaaatcaac atcatgactt cctctagctc tattatgtcg aatggtagaa
     1621 gccattcgat agtctaatga cttatcaata acagaaacag gtaatctccc actttccctt
     1681 tcataaatat ctgaataatc tagcattata cgatatctat gaaaaccatc aacaatacta
     1741 taccgatcct cttccttatc gtaatagcaa acaatcggca tagtataacc atcagatttt
     1801 atactatcat acaataattt catttcaggc ggagcaactg aattcggatt ataatcattt
     1861 ggagaaatct tttcaattgg aattgattta atctcataga ctggactaat aaaattactc
     1921 actttctaac tcctttaaac tcgtcttata tttctctttt aaaaaggcta ctattttttg
     1981 ttgctctctt gtcaaaccaa accccatagt tcgacaaata tgatcgtttt ttaaaataca
     2041 gaaacacatt cgtttccagc ttggtatatc ctttgttgtt acaatatcgt ctgtatcatc
     2101 aggaatttga tctaaaaaaa taacccttga atgtttaaaa gaggtataat ttgaaatacc
     2161 atttctagca atacgatatc ctttttcaat tagttcatta attgtttcct ccgcaagtcc
     2221 tccgcctgtt ttatgccaaa agtctattga tttattaaat tttctaacat aattatttct
     2281 taacctcact ggtagcgttg ataaaagaaa ctgagtgtac gacttccacg aatgaccctt
     2341 tggtaaagca attgatttat atcccattgc tttagtacgt ccataaatat tcgaaaagtt
     2401 aactccttga acacgtccca aaagctttac ccaagtttct tgatctatta ttctataaag
     2461 atttaggcta tcaacagcat agtcttgaaa aggagatgca actctcattt gtgatggttt
     2521 taaacccgct ttatagaata gatcataaag ttcgttataa gaataagaaa acttatagta
     2581 agcatgccat atgtcttcca ctgaccaatc atataaggga gatgctgtcc agacatcttt
     2641 gaattgtttg gtaatccagc attccttttg ataaccatac tttttattga taaaaccact
     2701 atatctgtgt aaggattctg aagcacggtt tcctaatata caaactgttt tttgatttcc
     2761 gtgaatttgt ttataccacc taccaaattg tttcgctaaa tcctcctgat tcattttata
     2821 acgataagta gtaattgaat tattttccaa attaataaca taatcttgag atggcattgg
     2881 tcttacccag atatcttctg ttttgtcatc ccacggatac caaaacattt cataactact
     2941 aagtgcagtt ctagtagcca tcggtaagca cacccaatac aaagaaactt tttttctacc
     3001 ttctaaagaa gtgaatgttt cttgaacata ttttgttgtt aaactgtact gtgcttcaaa
     3061 atcttgatga aacacaccta tttctctatc cggataatat ttatctctaa aatccagtaa
     3121 aatattaaga agtaacccac tatcttttcc acctgagaag gaaatataga caataggaaa
     3181 ttcattaaac aaaaaatgta atctttctat caatgcatca taaacatttt gatttaaata
     3241 ctttttcact aaacttcctc attatgcatt taaaaatatt aatacataga ttcactaaga
     3301 atctcttttt cgagcttttt gttttttaat ttttcaagtt gaataattaa accttgcttt
     3361 tctttttcaa ggtaatagac tctactcctt gtaagtctaa cttattctta atcttattta
     3421 tcgctcattt tttaaattcc gatgctctca cagatattat cattgtttag ttacatgtct
     3481 gtcaatctat tcgccaccac actaggggcc atctgattga aaaataaaat ttcggagtcc
     3541 attgtttgct caagaatcat atctgtcaca attacgtcat actctgaaga cagtgacacc
     3601 atatcaacca tggtcagcct caagtcatca aagttgataa tctcaaaaaa tcctccaaaa
     3661 tactctttta agaagttgcc aacattgtta taacttctct caaccactaa tagtttcaag
     3721 cgacgacgcg attgatttgt aagaaagaga ttttcccagg tgaggaacaa acaaatagag
     3781 gagatgattg atggttcttt cctttaattc cttatgatcc ccagcaaata actctttaac
     3841 ataagcaaca aaggtctggt attgaactgg gggtttttga tttgtaaagg cactatattt
     3901 ataatgcctt tatttaaggg ttaactctaa aaatcagatt ggtcttttgc ttggcaatcc
     3961 ggacaaatcc cgtaagcaat aactgggata cgcgtgacct tataacctgt ttgctcatgg
     4021 gcttctttgg caatatccat aacatcaaca tccataaagt cagcaatctt cccacatatt
     4081 tcacaaacta cattgacgtg ttgatggccc ataaagtcat agtaggtggt taaatcattg
     4141 ctgattttca actctgagac aaaaccttca tcaactaaaa ctttcaaatt attatagact
     4201 gtggcaaggc tcatgttagg aaagttaggc tgtaagtcac ggtaaatctt atcggcacta
     4261 gggtgttctg tggactgaat catgtaagaa ataatggctt tacgtgtctc tgtaattcga
     4321 atatgttttt cccgtagatg ctctaggaca ttttcatagg catctagggc ttgctgatgt
     4381 gaatgaatgt ccataagctg ctactcctat ccttttttcc tttattatca cgcacgtctc
     4441 tagtttcaaa gatttgtgat gcattctggt ttagcgacta attgcaaata gtctattttt
     4501 aggcagcgat tcatcacaat agcctctttg cctgccgacc gtaaaatggt ttgtacttct
     4561 tggttttcaa ggcctaattg cgcccaaaag actttggcct gacctgctaa aaagtctctg
     4621 gcaacttctg gtaaaaactc acttctgcga aagacatcta caatgtcaac ctcaaaggga
     4681 attgccttga tactggcata gaccttctct cccaaaataa gctgacctgc taatttggga
     4741 ttgactggaa tgatccgata atccatagcc tgcataaatt tggcgacacc ataagctgct
     4801 gtgtctttgc ggtcagaaag accaacaacc gcaatggttt tggctgactc caagtaagct
     4861 tttaggatat cctctgaggg attttgaaac gaataaatca cttgttcccc tatctattta
     4921 ataacctcta cagctgttca atatagcagg ataactccta aagcatggct tcaatcactt
     4981 ttaaccttat ccgtcaaaaa gctcaaagca gcttcatgac ttctcctaca attttttagt
     5041 ttattataac ataagttgtc aaaatgagaa ataacaagta agaaaactta tttggcttcg
     5101 taccagctct gcccagtact ttcatcgaca cataaaggaa cagctaaatc aacagcagct
     5161 tccatggtgt ctttgacaag tttttttata gcagtcaact catcatttgg cacttccaaa
     5221 acgatttcat catgcacttg aagcagcatt ttagctctaa aaccacctgc ttgcaaggcc
     5281 ttatccagat taatcatagc gattttgaga atatcagctg cgcttccttg aatgggcgag
     5341 ttgatggctg tacgctccgc aaaagagcga acgttgaaat tacgagagtt gatatccggt
     5401 aattcacggc gacgcttaaa gagggtctct acatagccct tgtctttagc ctcacgcacc
     5461 acattttcca tataggcttt aatcccagga tagcgttcaa aataggtatc aatgtaagac
     5521 ttggactgtt tacgggtgat accaaggtta tttgacaaac caaaatcaga aatcccatac
     5581 acaattccaa agttaacagc cttggcatta cggcggtcat tggcagtaac atctgctgcc
     5641 ttgtcaatcc caaacacacg catagccgtt gaggtatgaa tgtctgcccc ttcattaaag
     5701 gcagcaatca aatgctcatc ccctgaaata tgtgctagca cgcgcaattc aatctgtgag
     5761 taatctgaac tcaacaagac ggcatcttca tgagatgggg taaaggcctt acgaatcaaa
     5821 cgcccctgtt ccaaacgaat gggaatgttt tgaagattag ggtccacact agacaaacgc
     5881 cctgtctggg tgagatcctg cacatagcgg gtatgaatct tgccatcagc cagaatataa
     5941 tcctgtaaac caatcacata ggtggattgc aatttagtaa tctggcggta atccaaaatt
     6001 ttagccacaa ttggagcaat cggtgccaaa cgttccaaaa catcaacagc tgtagaataa
     6061 cctgttttgg tcttcttagt catctcaaga ggtagttgca tcttctcaaa caagatactg
     6121 cccagctgtt taggcgaatt aatgttaaat tcttggccag ccatatcata aatctcttgg
     6181 gtgagcgctt caataatgac cttgttttgc tctgccatat cttgcaaagt cgccctatta
     6241 accttgatcc cttcaatttc cattttagca aggacattag ccaaaggcaa ttcaatattc
     6301 tgatagaggt ctagttgctc atgggcagtg agcttgtcta gcatttgaga cttgctatct
     6361 aataatactt tgaccttacg agctaagtgt tctagtaaga cgtctttatc aggaacagcc
     6421 cgtttagcac ctttaccata gaccgtatcg tcttcttcca aagagatgtc agtaaaaatg
     6481 cgggcaatgg tgctaagctc attgtcttct acagttgaca gaaggtaatt agctagtcta
     6541 gcatcatagc taggtgctac caactcaatt cctaagtggc tcaacaagac cttgctacgt
     6601 ttaaaatcat aagtggcaat gggtttttgg aagacctgct taaaactatc cgtcgctaat
     6661 aggctaaggt ctgtggatgc gtaaatctgc tcaccatgtc cccaagcaaa accaatgatg
     6721 gcctcacgat ggtaattatc ccgaagcgtt tcaaagtaga agatgtcttc tgcgctaaac
     6781 aaatcagcag acacattggt cacttcttga taggaaatga cagctactgg ctcttgaggc
     6841 atctggctag caagaccttt cttgagttgc acaaaatcca tctcatcgta aaattgagag
     6901 aggctagcaa catctggacc attgtagaca atatcatcta agccaattgt gataggagat
     6961 gctgtattga tagtggcgag cgttttagat aggaaagctt ggtcacggtc attgatcaag
     7021 ttttctttca tttttgaggt cttgaatcca tcaatatgct cgtaaatgcc ttccaaacta
     7081 ccaaattcat gaaggagctt aaggcctgtt ttttctccaa tttttgtcac tccaggaatg
     7141 ttatccgatt tatctcccat caaggctttg aggtcaataa actggtttgg cgtcaagccc
     7201 attttttcca tgaggtaagc cggtgtgaat tcttcaaatt cggcaacccc ttttttagaa
     7261 atttcaacca cggtattttc gtcagttaac tggatcaaat ctttatcacc actgacaatg
     7321 gtcacatcaa aaggcacttc tgtgcgctcc gccattttat caagcgtccc gataatatcg
     7381 tctgcttcgt agtgttccaa ctcataataa gcaatcccaa gggctgtcaa catctcacga
     7441 atataagaaa actgctcacg aaattcatct ggggttttag cgcgacccgc cttataatcg
     7501 gcatacattt ctgttcggaa ggtggttttg cccgcatcaa aagccaccag cacgtgagta
     7561 ggccggactc ttttcatcat gtggtctagc atcaaatgaa agccataaat ggcattggta
     7621 tggagaccgc tatgattttt aaagcggtca atttgattgt aaagggcaaa aaaggcacga
     7681 aaggcaacag aggagccatc aattaatagt aatttattct tgttttccat agccttatta
     7741 taacacagag ggacgggctt ttctccttta agccattggt tttgctgata agtaaatggt
     7801 taacctaaaa agcctaggaa aattcctaga cttctgtatt tgctatttag acaaaagcac
     7861 ttattttgtc caacttgata ctttagattt gtttgcctct acccattttt tggcggcagc
     7921 ttctggactc atgcctttat tgatgtcaag catgactgct tccatatctt tttgggtcca
     7981 gtggaactta tcaatgattt tgtagacatt tggaagatct tttttcaagc ctttacgagc
     8041 aatggtattg atgttctcag tcgaaccaaa ggtttcttta ggatctttaa ggtacttcaa
     8101 gtcatacttg gcaaacatcc agtgtggtga ccaagctgtc acgacaatag gatctttttt
     8161 cttgatcgct tgatccaagg aagtcgtcat ggctcctgtt gatgccgcca ctaactccca
     8221 actagataga ttatgatact cttttagagt ctttttggct gctgccatga tacctgcacc
     8281 tggttcgatc cctgtgattt tttgatcggc ttgtttgctt aaatcctcaa tactattaac
     8341 atctgtcatg tatttaggaa cagctaaacc aagtttggtc ccttttaggt taggtcctaa
     8401 atcatctaat tttgacttgt atttctgata ttgttggcca tgggtgactg gcaaccaagc
     8461 actagttgaa aaatcagcgt tcccatttgc aacggtttgc cacatgacag cattgtcaag
     8521 cggtgtcaag gttacatggt aaccttcgtt tttcaaaact tctgcaatca catgagttga
     8581 ggcaacctca gaatcccact gcacgtaagc gatgttaaca gtttcaccct tatctgccat
     8641 accagaagcc atagtcataa taccgcgtcc taaagcagct atcaaaaaga cagcaagggc
     8701 tgctagacca atccatttat tggttttgcc agctttggct ttttcttgag gtttgctatt
     8761 gaacaactgt gtcatacggt caagaacaat cgccaagata accaaggcaa gaccactgac
     8821 aaatccacta ccaatatcag cgtgttgaag ggctgacaag acttcacgtc ctaaaccagg
     8881 agcaccaatc atggagcctg ttactaccat ggaaagggct aacatcatgg tctgattaac
     8941 acctgccata atagtatttt tagctaaagg caattcaact ttaaagagct tttgtttccc
     9001 agttgaccca aaggcatctg aggcctcaat caattctgtt ggaatgtcac gaatggccaa
     9061 atttgtaaaa cgaactgttg ggggcaaggc aaagatgact gaagcaaata ctccaggcac
     9121 cataccaata ccaaaaaagg caacggcagg aatcaagtaa acaaaagccg gcatggtttg
     9181 cataaagtcc aaaataggat tcacaatctg acggacagtg gcatttttag ccatccaaat
     9241 ccccagcgga ataccaataa gtaccgaaat caaactagct actaacacca gagtaaaggt
     9301 attcattagc tgcttccata acccttgatt gtaaataaag agcaatccta acaaggtaaa
     9361 ggttggcaaa ggccattttt tcttagcaag gaaaaacatc cctgctgtta ctagaacaat
     9421 aaaaagtaac gggtggataa ataaaagtgt tttggtcatc caatccatca aaaagctacc
     9481 aactacttgc ataatatcaa atagacctga gaaagtttta gttagccact ccgttagctg
     9541 ctccaccagt tgagctactg gtaattttgt ttgtaatatc gtttccaaaa tgcatcactc
     9601 cttttagtta aggtcttcgt ctgagatatt agccaaggct tcaatcactc gtcctcgaat
     9661 aatgacacct aacagacgat tattatcatc tgtcactgca atcggagcag atgaatcata
     9721 gataagaggc aaaatatctg taataatagt atcttttgag acagttctca catcgcgatc
     9781 aatcacttct gatagcggta accctttttt gcgcgcctct atagcggcat cggccgtcaa
     9841 actgccgact aattggcggc ggcgattcgt cgccatcaac atagacacct cttcgttgtg
     9901 catacggttc aaggcaactt gaggtccatc taattcaaca gtagttgtta acggtttgat
     9961 catgatattt tgtgctgtca agactttaga acggtccaca tcctcaacga attcacgcac
    10021 aaagtcattg gctgggttag tcaagatttc ctcaccagta ccaatttgca taatttgtcc
    10081 gtctttcatc aaagcaatcc gatcaccaat ccgcaaggct tcattcaagt catgactgat
    10141 aaagatgatg gtttgtttca tgctgtcttg caaatcaagt aattcatctt gcatctcacg
    10201 acgaatcaaa ggatcaagcg ctgagaatgc ctcgtccatt aagagaattt tagggctatt
    10261 agctagcgca cgggctaggc cgacacgctg ctgcatccca ccagatagtt ggtttgggta
    10321 ctggtcttta aaatctaata ggcctgaatt atcaagggct ttttctgcca atcgctggcg
    10381 ttcttctttg ggaacgccac gtaattccaa accaaattcg gtattttcca aaatggtttt
    10441 atgaggaaag agggcaaagc tttggaaaac catgttaatg tcatggcggc gcacctcacg
    10501 cagctgatct gctgacatgg ttgagatgtc tttaccttcc agcaagatag atcctgctga
    10561 aggttcaatc aaacgattta gcatgcggac aagggttgat ttcccacttc ctgatagccc
    10621 cataataaca aagatttcac cttttttgac ctcaaaacta gcgtcataga cacctacagt
    10681 agcgcctgtt ttcttaaaaa tctcactctt atttttgcca gtctttacca tctcaagagc
    10741 tgctttttgt tttttgccaa aaattttact gagatgtttg acttctaaaa ttgtttccat
    10801 tcgttttttc tcctaggtaa tcgtttatca tgtgaaataa tgacatagct ttggcgaaat
    10861 attagccaat atttaacaaa gtagctctac acaattaata aacgtgtggt cactttttta
    10921 cttaaaaaaa gaatctgccg aaacagaaca taactatatt atcacgtgtg gtcacttttt
    10981 gtcaaggaca ataactcgat ctggtaatgc cacaatgata ggtgatcgct ttcattcttt
    11041 taggtatggt cttttaacca gtacaatttt ttatcacaac tgatggctat ttctttgctg
    11101 attaggctat tgttacatta gaaatccttc ttttacctat aatatctctt ttgcggccat
    11161 cgtaaataac gaccacggtg actcttaaac atttgctcat ctctccaaaa aaactcacct
    11221 aagacagtgt cctatgaagc gagttagctt aatcaggcac tatcatctta ggtgagtagg
    11281 gtgttgctta tagtaaagct ttaaattgga tattagaatc ttctaagaag cagtcatcaa
    11341 atccacgagg atgatggtat tcgatacgat tttggtcttg tggataggtg tacttgccac
    11401 caacctccca gataaaggga tggaaatcgt attggagacg ttctttgcgc attttccaca
    11461 gttcaaggat ttcatctgtt gatgccataa agtttgacca aatgtcataa tgaactggga
    11521 taacgacttt agcacggaga ttttctgcca tacgaagaag gtcaacagaa gtcatcttgt
    11581 cttgaattcc aattgggttt tccccatagt tatttaacac aacgtcaata tcataatcac
    11641 gtccatgttt agcaaagtag ttcgaaaaat gagaatccgc accatgatag atggtaccac
    11701 ctggtgtttc aaaaatatag ttaacagctt tgcgagccat gtcatcatct gtgatagcta
    11761 aacctgctaa atcaccatct tgagcatcag ctccttgtat tgggagagtt accagacagg
    11821 tgcggtcaaa cgactctaca gcggtgatct taatatcttt gaattcaaag ctatcacctg
    11881 gctttaagat cataatacga tcttttggca caccccaatt tttccaaact tcaccacatt
    11941 cataaggtcc tacaaattta acatgattaa gttttgggtt attgataatg gctgccgcag
    12001 tattgatatc gatgtggtca ctatgataat gagaaaccaa ataataatcc aactcattaa
    12061 tcataaatgg atcaatcacc attggttggg ccctaagatt gggctgtaat ttacgagctc
    12121 ctgccatatt agccatttga tgtccacgaa ccatatcctt aacttgcttg gtcgctttcc
    12181 cacgatttga ccataagtcc atgacaacat tagcaccgcc tggtgtctta atccagatac
    12241 cacagtttcc taaccaccac atcgcaaagt tgtcagctgg cacgacttct tgctcgattt
    12301 cttcatttaa ccatgttccc cattctggaa acgtgttaag gatccatgat tcacgtgtaa
    12361 tatcttgtac tttagtcatt agggtttcct ccgaatttgt tcttcgtttc tatactttaa
    12421 gtatagactt tcttgtcaat gataaaaaga ccaagtgtca cacagatctg tgttcaaaag
    12481 tggactgact ttttcattag ctcagtctaa tactgtctca ttttgcacac tgtatcttgc
    12541 atcaatactg tattcaaagc tatattaaaa acctcagtta tatttttcta tgtcatagaa
    12601 aactgaggta ctttgacgac ttaacactta ctcatagcag ctcgcgatga atgagttttt
    12661 caattttaga aaccaaggca taacgatctg attcatcttt tacaacagct tcaatagctt
    12721 ttgtgagctc tgtagaaaag cgggagtgct cactcaagcg tccttgcttt gaaaagcgta
    12781 tcaatttgat gatatcatcg tcacttaaaa tagggttaac aataagcatc gggattttag
    12841 tctttagcgt atctgtagta gcaacaatca tatcgactgc taaaaggtcg taaacactct
    12901 gatactgttc tgtcgtaaaa acagcttcaa tctgaccatt tgctagatac ctttgacact
    12961 gtttgaacaa taacttttga atgcctatcc catcatctga aacaataacg agtttggtct
    13021 tttcttgctc tgtattattg tgacgcagct ctcccccaag atggatagtc aagtaggcaa
    13081 tatcatcgtc tgtcaagcta atcgtccaat cttgttccaa aatagtggca caagattgag
    13141 tcatagcaaa caattcttcg tatttttctt taatatggtc tgttaaggga ttaactaaaa
    13201 aaatcccata agctttacgg taaacgagcg ccttacagtg ggtcgtcaac tgcttcagca
    13261 aatcctgttt atgcgtgaaa tgtaactgat aacggctctc caactggtca ataaagtgac
    13321 taatggttgc tcgcatgtcg tcataatctt gactttccac gtgatgatct tgatcttttc
    13381 gaaaagacaa catcaacatc gctaccatac taacttcgat atcatccaaa tgaagcttaa
    13441 aattatggta aagctctcta gctaaatcct gagcaatgtg atattcttta cgtttccaaa
    13501 tcaaatgaaa ttcttgttta agagcagact ttgtttctga atcaagacgc atattacgat
    13561 aacttaaaag catgaaaggt aaaatctgca acatgaattg gctatcttgg gtattaattg
    13621 tctttcctaa actagcttga gataaaaaga gatactcatg aaaataggtc aaaatatctt
    13681 tagaaaaata aaccgataag ccttgtatct ctgagagttt acggttgaat atatcaataa
    13741 agctagtatt gccaccttgg taaacgtcca ctaacaattt gtacaggtat tgaataagag
    13801 ccattggatg acaaccaaaa taatatcccc tagcctttgt cgcatgaagc tgaatcttgt
    13861 attgtttgtc ttccagttct tcccttaact cagtcaggtc atttaaaatc gtgttacgag
    13921 acacatcatt gatctgcatc agtttatcaa tggtcacgcg ctcagtggaa accgcaatat
    13981 aaatcgagga taggcgtctg cgctcatcac ttttcatgac ataattataa tcagtcactt
    14041 cttctaataa taagcgacaa gcagcttttt gatcagctgt taaaagaatg ccaagtctag
    14101 gataagagat aatctgatct actccctttg gcaaagcctg attaattttg tcaagctggt
    14161 aataaacttt acgacgtgac tgatttaaag catgagaaat ggccataact gtctcaggtg
    14221 tttctagttt aagtaaataa gacagcaagt cataactctt tttatctaaa atcatcatgt
    14281 ttacaaacct cctctctgaa aatccatcct tgcttatttt atcatgattt cagtgactgc
    14341 gcagccaagt tttgaaaaac tcaccataat aagattcgct taatatgatt cgagctaatc
    14401 acctcaactg atccaacacc aacaccttat atctggtctc taagaactcc acaacatacg
    14461 attcatccag tttgtgctca ttggcgttta aatagttcat cgaaaaggac aagtcaaaag
    14521 gacttgccca aacaatgatt aacaaaacct tgaactttct ggcttctagt gagaacctgt
    14581 gctaataagc catatcgtga cctcattttt gaccgtaata agcatttggg ccatgtttac
    14641 gcaaataatg cttatccata atgtatcgtg gcgctggctc tacgcgtgga ttgatttgtt
    14701 ctgtcaagcg attcatccta gccacttctt ctaagacaac cgagtggtaa acggcttgct
    14761 ctggggtttt cccccacgta aatgggccat gattgcgaac cacgattcct ggaaccgcca
    14821 taggatctaa gcctcgcttg ctaaactctt ctaaaatcac gttgccagtt tcttgctcgt
    14881 aagccccatc aacttctgct tttgtgagag aacgagcaca cggaactggt ccatagaagt
    14941 agtcagcatg cgtagtccca taaaacggaa tatcacgacc tgcctgagcc caaccaacag
    15001 cttctgtgga gtgagtgtgg acaataccgc ccacttctgg ccaagcttta taaagttcca
    15061 catgggttgg cagatctgaa gaagggttca aatcaccttc gaccacattc ccatcaaggt
    15121 ctgtaacaac catgttttct ggtgtcaaga ggtcgtagtc caccccagaa ggcttgatga
    15181 caatacgtcc taattcacga caaacttcag agacattgcc ccacgtaaat ttgacaagac
    15241 cgtgttgtgg tagagacttg ttagccgcac agacacgttc acgcatttct tgcaagtttt
    15301 tcgccatatt atgccaaccc cgctttctca ataagtgggt aaaggaagtc ctgagcttct
    15361 ttgatagcag cttttgtttc ctccaccgtg tcacaatttt ctgaccacat ttcaatcaaa
    15421 aaaggcccat tgtagttggt tttcttgagg accgcaaaca gctcttgcca gtccacacaa
    15481 ccttgaccaa atggcacatc acgaaactgc ccttttgagg tttccgtgac agcataagta
    15541 tctttcaagt ggagggctgc aatggacttg tgaccattgt aaaattcact ccacaaatca
    15601 ttatgccagg cagaaacatt gcctgcatca ggataaacaa agagataagg cgagtcaatc
    15661 tctttttcaa ctgccaagta tttttcaata gagttgataa aagggtcatc cataatctca
    15721 atagaaagca tcacttgggc ttcttcggcc cagtcacaag attgtctcaa gtttttgata
    15781 aaacgagcac gggtttcagg cgatttttct tcatagtaaa cgtcataacc agctaattga
    15841 atggtacgaa cgcctaagtc ctgtgccaat tcaatacatt gtttcatcag tttaagggac
    15901 ttggcttcaa tagcaggatc atttgagcct agtgggtaac gtctatgacc agaaaaacaa
    15961 atggtcggga tgcgaatgcc tgtttcataa atggctttca ctaaatcaag ccgttcttcc
    16021 ttagtccact ctaagcgcgc taagcgagca tctgattcat caacagacat ttccacaaaa
    16081 tcaaaaccta agtcttttgc aaattgcaag cgttcacgcc aggtaaactg ttttggcgtt
    16141 gctttttcat aaataccaat aggacgcgcc ataaagttac ccccagatac gtttgatttc
    16201 atctttgaag gcacgcgccg ctgcagcagg atcttctgct tcagtaatgc ctcgccctgc
    16261 gataaaggta aagacatcaa ccccttcaaa aagtttcaaa gtgtctacat caagcccacc
    16321 agttactgac acgcggaagc ccatgtcaat caatgttttc accttgttaa ggtctttttc
    16381 accccaagtt tctccagcaa gaagggcatc acgtgattgg tggtaaatgg cttgggaaat
    16441 cccagcgtca agccataatt gagcttgctc ataagtccaa tcaccataaa gttcgatttg
    16501 aatttcccca cggtcaccgc gctcttcttt catagccttg agggctgctt ccatggttgg
    16561 aatcgtcgca cagcaaatgc aggtcatcca gtcagcacca cgttttgcat tgtttttagc
    16621 gaccgtaccg ccagcgtcag cacatttggt atctgctacg ataattttct ctgggaagag
    16681 gctgcgtaat acctcaacca actcgcttcc gacttgaagc aagcagaccg taccggcttc
    16741 gataacgtct acttcgtgac ccacagcaac ggctgcttta acagccccct gtaagtcaga
    16801 atggtctaaa gcaacttgta aatttggaat atgtgtcatc tctttttcct aaaactctta
    16861 gccaccgtgc aaactggtta agagttctta tcctttccat cattcgttag tatcctgtgt
    16921 aaggaaagct taagcattca agtccatacc ttctaagtaa ggactatctt tagattcttc
    16981 taccatttct agaacctctt taggactttg gcaggccacc aagcgttcaa tggcgttatc
    17041 tagttcaaac aaggccacaa tttgtgggat agccactgtt gtgtgaatag aagggtctgt
    17101 agcggccaac gtcaaaagaa cagaaacctc tttaccatct gagaaggtaa ctggttttgt
    17161 tagtgtaatc aaagcaaatg cattgcgatt aacgcctaaa cctgcttctg catggggcat
    17221 agccatacct ggcatcaaca cataataagg gccgtatttt tctgttgatg caatgatggc
    17281 gtcgtagtaa gctgacgtca ctgctttgct atcaatcaaa ggttgaacag ctaattgaac
    17341 agcctcttgc caagtatcag cacttagccc caatcggatg gagttgttgt caataaaggc
    17401 ttgttttaaa ttcataaaaa ctatctcttt ctagtgtctt gctttgcaac acacaccatt
    17461 tttactagtt ttatcctact aaagagaaag agctgaaaac cttcgtcaca actccttcgt
    17521 tttgattcag aattagctta tagcacttct tgtagtttgg ttttaatttc attatcatcc
    17581 atcaggttat caagaccaac caagtggcct ttggtacgac catcaagctc atgaatcaag
    17641 tgatttgatg ccactacaat atcatagcca gaagctagac ctttagcttc accaactgag
    17701 caagaagctg actgaatatc tgtcacgcca agttgacgaa gcgcattttc aactttcatt
    17761 ttgatcacca tagatgaccc cataccattg ccacatgctg ttaagacttt aaccattgtt
    17821 ttttcctccg aaaatgtttt attattgttt tgttaaaata gcagagctat ttcttaagat
    17881 gcagtgttat tgagcttccc cacggtaata agcttctttg tccttagctt ttgcgaattg
    17941 caattgtgga atggcaagta agaagatgca aacaagcaca taccctgcaa taccaaggaa
    18001 tttgaagagg tacccaaatg gtaaccatgg aagaaccaag tcgatattac cgtgataacc
    18061 acctgtaagt cctaaaagac ctacagcaac agcaccaagt gcaacttgca aaatacctga
    18121 aataaatgat agagctaccg ctgctttcca gccaccacgt ttatctgcat aaaccgcaat
    18181 tgccgcatta tcaaagaata cgggaacgaa tcctgtgata attaagattg gatttttaaa
    18241 gataacaaga agagcaatgg tgattaattg accaatcaaa ccaaaggcaa atcctgaaag
    18301 caccgcattt gaagaaccaa agccatagga agctgcaacg tcaaccgctg ggaatgaccc
    18361 tggaagcaat ttgcttgaga tcccttgaaa ggcattggtc aattcagaaa caaacatgcg
    18421 aacaccctgc atcaagataa agagataaac tgagaaggtg agagaggttt gcaaaatata
    18481 catgaagaaa gcttgcttag ctgggttgaa tgctccaggt ccgattaagt ccacatcaga
    18541 cataatgtct ggaccaagga cagccaagat agctccgaaa aagaccaaca tcaaagttgc
    18601 tgacgcaaca acagtatcat ggaaaatgtt taagaaggtt ggtaatttta ggttatcaag
    18661 gttttcttct tttttaccaa agaacggtgc aaccttatcc acaaaccaaa tcgcaaattg
    18721 ttgttggtga ccaatcgcaa acccaccgcc acctgtaagg cgttgagttg cttcaacggt
    18781 catgtttgag ctgatagccc agtaaagccc acagatgata ccgactgccc atgccccaaa
    18841 agcattttgg aattgaggga ttaaaagaag gacaaagacc gaaatcgtcg ctgcctgttg
    18901 gaccatgatg tgacctgtga tgaaaagggt acgaacttta gtgaccttgc gaagtgcaac
    18961 caaaagaatg ttgaccccaa atccaatcaa gagagctgtc gtagcaacac tgatgaagcc
    19021 catctcttct aacttagtat tagcagcagc caaaccaaaa taaggatcaa taacggcggc
    19081 tttcaattca aatttcttgg caagagcaac caagatcgga cgaaaagtcg ttacaagacc
    19141 gcctgctcca acgttcaaaa tcaagtaacc aacggtagcc tttacaaaac cagcaaagac
    19201 ctcataaatg ggttttttaa ggagtaaata cccgattaaa accaaaagac ctacaaagaa
    19261 cgctgggttt tgcaaaatat tttgcgagaa ccagtttagg ggagctagga gcatttccat
    19321 aagaactacc tcttttcaaa gttaataaag ataaagtgtt tatatgttca ttataggtaa
    19381 tgaaagcgtt ttaaaaaaga ccaactatca cacaaagaag tgttcaatat tagacttaag
    19441 ttaacttatt ttaaaattta actacactta ataataagga gaaaatcacc ctaaacaacg
    19501 attaaaaacc acaagaacct tcgttcttgt ggtttttcta gatgagttgc tttttgaata
    19561 gatcagctct ttggcattaa ctgtgacgtg acgcaaataa ccactgaagg gctgtgtcac
    19621 ctcaaactat ttgataacaa tattaacgag tttgtttggt actgcgatga ctttgatgat
    19681 gtctttgcct gcgatttcgg cttggacttt ttcgttagca agggcaactt cttgtaattc
    19741 ttcgcgtgaa aggtctttag caactacaag tttagctttg accttacctt tgatttggac
    19801 aacgatttcc acatcgtttt caaccaattt gctttcatca taacttggcc atggcacgta
    19861 agagattgac tcacctgagg ctgtcaaggc ttgccagagt tcctcaccta agtgcggtgc
    19921 aaatggtgca atcaattgaa caaagccttt ggcatagtcg ctaaagagtt tgtcttcctt
    19981 gttggctgca ttgacaaata ccatgagttg agcgatagct gtgttgaatt tcatttggtc
    20041 aacttgctca gtaacggcct taacggtttc gttgtaaacc ttgtcaaggg ctccgctatt
    20101 ttcctcagtg atctctttag tcgtgatcaa acggtaaaca cggtccaaga acttacgtga
    20161 gccttcaaga ccttcttcag accaagcgat ggaagcgtca agtggtccca tgaacatctc
    20221 gtaaacacga agggtgtcag caccgtactg ctcaacaaca tcatctgggt tgacgacgtt
    20281 cttaagtgat ttagacatct tagctggtgc ttgttcaagc tcttccccag tttccacatg
    20341 gaagaagctg ccatcacgtt tttcaacctt gtcagtcgct acaagcgctc cacggctatc
    20401 gcggtagctt gtccccaaaa tcatcccttg gttaaagagt ttttggaatg gctctttggt
    20461 aggtacaaca ccgagatcgt aaagaacctt gtgccagaag cgagcgtaga gaaggtgaag
    20521 cacggcatgc tctgcaccac cgacatagat atcaactggc aaccattgtt tgaggagttc
    20581 ctcatcagcc agtttttcgg tgttgtgtgg atcgatgtaa cgaagataat accagcttga
    20641 acctgcccat tgtggcatgg tgttagtctc acgacgacct ttaacaccat cttcacgggt
    20701 aacttccaac cagtctgtca cgttagcaag cgggctctca ccagtacctg aaggacgaat
    20761 atccttagtt actggaagaa ccaatggcaa ttcactctca ggaaccgctg ttgatgtccc
    20821 atcttcccag tggatgattg ggattggctc accccagtaa cgttgacggc taaagagcca
    20881 gtcacggaga cggtaagtga cattttcatt accgacgcct tctgcttcaa gccattcgac
    20941 cattttggca atagctgacg ctttgtcaag accatctagg aagtcggagt tgatgtgaag
    21001 accgtcttct gtaaaggcag cctcttcaac attaccacct tcaagaacag ggatgatgtc
    21061 taggttaaat tgttttgcga attcccagtc acgttcatcg tgggctggaa cagccatgat
    21121 agcaccagta ccgtagctag caaggacata gtccgcaatc caaactggca tttctttgcc
    21181 gttgaccggg ttaatcgcat agctacccgt ccagacacca gttttttctt tggcaaggtc
    21241 tgtacgagca aggtctgatt tgagacttgc ttgacgtttg tagtcagcca cggcttctgc
    21301 ctggtcagat gtcgtaatag catcaaccaa ggcatgctca ggagcaagaa cggcgtaagt
    21361 tgcaccaaaa agcgtgtcag gacgtgttgt aaagacagtg aagtccttat ctgtatcctt
    21421 aaccttgaag gttacattag cgccagttga tttaccaatc cagttgcgtt gcatgtcctt
    21481 gattgattct ggccagtcaa cttcttccaa gtcttctagc aagcgctctg cataggcagt
    21541 gattttgagc atccattggc gcatgggttt tctgacaact ggatagccgc cacgttctga
    21601 tgtcccatct ggaagaacct cttcgttggc gatagctgtt cccaattctt caacccagtt
    21661 aacgggaact tcagcttcgt aggctagacc tttttcataa agcttggtaa agatccactg
    21721 cgtccacttg tagtagttag ggtcagttgt gttaacttca cggtcccagt cgtatgagaa
    21781 gccaagagca ttaatctggc gtttgaagtt agcgatgttt tcagctgtaa actcggctgg
    21841 atcattacca gtatccatag cgtactgctc agcaggaagt ccaaaggcat cccaacccat
    21901 tgggtggagg acattgtgac cttgggcacg tttgaaacgg ctaagaatat cagttgctgt
    21961 atagccttct gggtgaccaa cgtggagacc agctcctgat ggatatggga acatatctag
    22021 ggcataaaac ttaggttttg atgcgtctgt tcctgtttta aaggtgtgat tatctgccca
    22081 aaaggcttgc catttgggtt caatagcggt gtgatcgtaa aatgtcatgt gaaatctccc
    22141 atgtaaaaaa tctagtcttc ttattatagc atgagatgaa gcttttagga atggtcaaga
    22201 catgactagc accttggctt ttgagacttc agacgattag ctatttttaa accctgcttt
    22261 agcatcaacc atcctcacaa aaagctaata aaaaagccag acttgctctg taaacagagc
    22321 caatctggac aaatagactt aaaaagcaag tgcttgctca aaatcggcca ataagtcttc
    22381 gatattttca acaccacaag acaagcgcag caaaccatca acaacccctt gttccaaccg
    22441 cgcttgtgac gataaacaag catgagacat cgttgctgga tgtgacaaaa tggactctac
    22501 tccacctaga ctaacggcta aaatcggcaa ctgaatacga tgggtaaagg acatcagctc
    22561 ctccttagaa gccaactcaa aggataaaac agcaccacca ttcttagctt gttgcaggtg
    22621 ggtatcgtga ttaggatgac tgtctaagcc agggtaatgt accttaagaa ctttggggtg
    22681 cttcgctaaa tagttagcga gctgctgagc attttttacc gcttgttcca tacggatccc
    22741 catagttttc atgccacgaa gaatcagcca agcgtcttcg accccaacaa ttgctccaaa
    22801 gtttttttga aaaagcttga gctggttgta gatagcctca ttattggtag cagctaaacc
    22861 agctaccaca tcactatgac cattgataaa tttggtcaca ctttctacta caatatccac
    22921 acccatagct aagggttctt gatacaaact ggtcataaag gtattatcag ccacagttag
    22981 cacaccataa gctttggcga gttgcgcaag ctctgaaata tcacaaattt tcaagagcgg
    23041 atttgacggt gtctctagat aaatcatacg cgtattaggc ctgatacttg cttttactgt
    23101 tgctaaatca gccatatcta caaagcttgt ttcaatctga taattgggta aaatagctgt
    23161 cgcaaattga caagtccctc catagacctc caatgggaaa atcacatgat ctccagcttt
    23221 taagagcatc aaaacagttg aaatagcagc cataccagat gcataagcca ccgcataagt
    23281 cgctttttct aaacaggcta acccgtcttc taaagcttca gtcgttgggt ttgaaaagcg
    23341 tgtgtacaaa tgtttttgac taggacaata cagttctgaa ttgtggaacg tcgaagtctg
    23401 atagatggga actgaagctg ctcctgtaaa ctcgtcaata accgtatagc catgtaataa
    23461 ggtcgtattt tctctcattt cgtgcacctc cgcaatagcg ttaactccaa tgaactctct
    23521 tttagacaat ggtcccaccc caatggttgc acaaaccaaa aagctcttca aaaaagcgtg
    23581 atctgctaac ctataagaaa aataaagaaa agtagtgggc accttttaaa tggataatga
    23641 atttttccaa taatatcatt tttagatcga ttacgtttat tatacaacct ttagctaagt
    23701 ttgcaagccc ttttatcatc tttgaccaag ctgccaacac ctctccagca agttaaagta
    23761 tagctacctt tggccccatc gtctcaaaag ctcaaattat cacactgaca gtcctacctt
    23821 gcatcgtcac ctactcaata tggcaatact gccatcaaaa tttcattaaa aggtcacaat
    23881 ttatttcatt taatgagaaa cagaacttgt tttcaccatg cgtcttatag tatactgcta
    23941 aaatactatt tatttttagg agagaaatat agtgaaaaac aaattatttt tagttgccct
    24001 tgcgaccgta actgtcctag ggccctcttt agcaacccct catcatcaga ccgtgcatgc
    24061 ggctgagcag gcaaaagaac cccaaaccag ctcaatgacg tgtactaacg atggatctgt
    24121 atgcgctggc tggcatagtt tatcagaagg agacagtata acttcaaatt catcttcaaa
    24181 caccaagact tccctgttaa actggttatg gagcttaata acaaatttgt ggaattctgc
    24241 aaaaaacttg ttcatctaag atctctataa tcatatccat ggaatttaaa taaccatctc
    24301 gtggcggttt tctaatccta ggtcgccaca cttcttattc agataaataa taataaaaac
    24361 attaaaataa cacttgattt attaacaaaa agttagtata atataatcac aatacaaatt
    24421 taaggagtta caaatgaaaa agaaattatc tttatttatg atcgcaactg ctgctgttgc
    24481 aggccttagc cttgcaacac taggaagcca ccaaactgtt tcagcaaatg atgctagcta
    24541 tagtaacggt ttctacggac acactggtcc tgacagctgg ttactttaca cagtatggta
    24601 aacctaatac cgtaacatag aagctatctt cttcgtaaga ctacggttat gaaaagccta
    24661 gagctgcgta agcagttcta ggctttttga ttggctaaaa cgtttttact aacgatccct
    24721 caagattaaa aagaaaaggc ataccgccta taaaaggaca agcccaaaac acaagtcgta
    24781 acagttgcta gctaaccacc accaaaaaag ctgtaacacc tcaaatataa ttataaccag
    24841 attaacatta ttaataatta atgttattta tataagataa agaaatgctt tagtatcggc
    24901 tttctttgcc tttttcggtc acacacataa caaaaagatt aaaatgtgat attgaatcat
    24961 taacataata tgagtataat ataggtatac ta
//
LOCUS       DAUDYX010000027        24849 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.027, whole genome shotgun sequence.
ACCESSION   DAUDYX010000027 DAUDYX010000000
VERSION     DAUDYX010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24849
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.027"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..180
                     /locus_tag="VHD02_001070"
     CDS             <1..180
                     /locus_tag="VHD02_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEQ5497686.1"
                     /translation="DSLTRQVKNIYFLQNTKDDMLSFSDINVMPRTQDIEREFNVKEW
                     IGDAIVHVESKLNAR"
     gene            183..707
                     /locus_tag="VHD02_001071"
     CDS             183..707
                     /locus_tag="VHD02_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEQ5497687.1"
                     /translation="MNIEEAKRAIRELEAFNYFDFKGNLIKRIDVLGIIDQLGQPKVV
                     VPKPVFEMIKFRKEHDQDLTTTFVNIHAYPDNVFHWVANNGDLFAQAWLAYPNITIEK
                     EKLYTVEIPNPNREGRGHAKFVLERQGNKVFLVKRKSKDCYYNKNSNHLTESEIRKDF
                     DWAWREGFAEEVTE"
     gene            704..970
                     /locus_tag="VHD02_001072"
     CDS             704..970
                     /locus_tag="VHD02_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497688.1"
                     /translation="MKEKTIFISKKYANDFNNDKYNLSSGYYFKNGEQHDIAIVKYGE
                     QDYLKNTDLAYVVCDKIVDGESIGFVYHGEYETWRFKLLNTEAN"
     gene            1256..1690
                     /locus_tag="VHD02_001073"
     CDS             1256..1690
                     /locus_tag="VHD02_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEQ5497689.1"
                     /translation="MTFFPEIDIQKTKSNAKRKLREYPRWRRIANDVDTQKVTATYSF
                     EPRQPYGTPSKPVERLALNRVSAEQELDAIEQAVSMILEPERRRILYDKYLAPYKKAD
                     KVIYTELCMSESFYYDTLDIALLAFAELYREGVLLAEKGVFS"
     gene            2300..2680
                     /locus_tag="VHD02_001074"
     CDS             2300..2680
                     /locus_tag="VHD02_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497690.1"
                     /translation="MARANKAKMLDAIEFKLLNPTANQAQVAEIIGITDRQLRNWEND
                     PEDKSGWNKLNDEIAEKAFGKYALSAIGTLVNLSKNAKSELVRLQASQDILDRAGYKP
                     TDKQEISIDEPIVLANSWVQDNGS"
     gene            2670..3944
                     /locus_tag="VHD02_001075"
     CDS             2670..3944
                     /locus_tag="VHD02_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEQ5497691.1"
                     /translation="MVVDLADIIPIGFKPVVQATWNPQILNIACKGGRGSGKSSNIAF
                     IISRLIIQYPVNAVCIRKTDNTLEQSVYEQIKWAISEQGLERYFKFNKSPLRITYIPR
                     GNYIVFRGAQNPERIKSLKDSRFPFAIGWIEELAEFKTEDEVKTITNSLLRGELGDGL
                     FYKFFYTYNPPKRKQSWVNKKYESQFQPSNTFVHASTYKDNPFIAKEFIAEAEATRER
                     SERRYRWEYLGEAIGSGVVPFDNLRFERITDEQVADFDNIRNGIDYGYATDPLAFVRW
                     HYDKKKNGIYAIDEYYGQKISNRQLAKWLTTKGYQSDEMFAESAEPKSNAELKNEFGI
                     KRIKGVKKGPDSVEFGERWLDDLDFICIDPKRTPNIAREFENIDYQVDRDGNPKPRLE
                     DKDNHAIDATRYAMSDDMRATKTIVKTFKGGI"
     gene            3944..5269
                     /locus_tag="VHD02_001076"
     CDS             3944..5269
                     /locus_tag="VHD02_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEQ5497692.1"
                     /translation="MPELFIVPSDTEMTKDLLSELIQKHRSFNLSYSAYKQLYEGDHA
                     ILQQKQKEQYKPDNRLVVNFAKYIVDTFNGYFIGVPVQTSHENKQVSNYLELLDGYND
                     QDDNNAELSKICSIYGHGYELVFNDENAEVGITYLTPLEGFIVYDDSIRQKPLFAVRY
                     FYNKDGVLEGSYSDASKITYFKDGEKDLEIGESDPHPFDGVPMIEYVENEERQSLLAS
                     VVTLINAFNKAISEKANDVEYFADAYLKILGAELDDETLKSLRDTRIINLKDTDAQQL
                     TVEFLQKPDADATQEHLLDRLENLIFRTAMVANISDESFGTASGIALRYRLQAMDNLA
                     KTKERKFMSGMNRRYKLIASYPTSKIGPKDWIGIKYKFTRNLPANLLEESQIAGNLAG
                     IVSEETQVGVLSIVENPQKEIEKKNSDRPTLISRKAGGLNGQDTTTVLE"
     gene            5238..6146
                     /locus_tag="VHD02_001077"
     CDS             5238..6146
                     /locus_tag="VHD02_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEQ5497693.1"
                     /translation="MAKTPQQYWNERQKQLWANLETSERALQTKLSKYYADEAKSLEK
                     EIGAYFSKYGKDNVIEYRNLLQQLSKADKDLLYRDCERFAKKYPQRADLMPVRTSIYK
                     LDRLQGLELSIKIQQLEIGAIEEAELTKHLTTAFKKGYQETAKTIGFQADKVSAELFV
                     NNDWTGKGDFSSSIWSNKDKLVDYLTNDFKTAIIRGDSFDKVVKQMSERFTVRSQSDI
                     TRLIMTEGTYVNNQAMMAPFEDSDDFEEYEFVSVLDSSTSSICRGLDGHKFKLKDRQV
                     GVNFPPMHANCRSTFAMVIPDDYLER"
     gene            6153..6422
                     /locus_tag="VHD02_001078"
     CDS             6153..6422
                     /locus_tag="VHD02_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497694.1"
                     /translation="MNKRIKKKRKLETAIVMIVAENAMQAEALRNQNKEIMELKSIVQ
                     RNALATNEELATVKAATLDNQSAIKSIGDDVDYIKQNYKRKWRKK"
     gene            6667..7236
                     /locus_tag="VHD02_001079"
     CDS             6667..7236
                     /locus_tag="VHD02_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEQ5497695.1"
                     /translation="MENEEILEQSGAQEEAKEQTFDDILSDPKKQAEFDKRVAKAIDT
                     ARNKWVAETEEKENEAKRLAKMNAEQKAQHEKAKLEARIAELEAERTLSEMKSAARTM
                     LSEANINISDALLSQLVSTDADKTKNAVEAFSEAFSEAIEKEVKERLKSPTPKKSNGN
                     TGLTKEQILAVKDTAERQKLIAENIGLFQ"
     gene            7255..8145
                     /locus_tag="VHD02_001080"
     CDS             7255..8145
                     /locus_tag="VHD02_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEQ5497696.1"
                     /translation="MVTSRTYPEENLIKSTDLKYPITIDVTNKFQENISKLLEMLGVT
                     RKISVSEGMTLKTYAGYDVTLAEGNVPEGEVIPLSKVERKIHSEKKIELKKYRKATTG
                     EDIQMYGSNEAVTNTDNALVRQLQKKIRTDFVTALKTGTGTQDALGAGLQGALASAWG
                     KLQVLFEDYGSERAIVFANSLDVAEYIAKAGITTQTAFGLTYLVDFTGTVIISTNDVT
                     KGEIWATVPENIIFAYINPNNSELAKEFNLYGDPTGYIGMNHFQENTTLTIQTLLVSG
                     MLMYPERIDGIVKVTLTPGV"
     gene            8158..8451
                     /locus_tag="VHD02_001081"
     CDS             8158..8451
                     /locus_tag="VHD02_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEQ5497697.1"
                     /translation="MAYRVKARFFDLLDNSFLYEVGDSFPRKGYEPSKERLESLLSSN
                     NTEHKPFIEVSGNGNSLDGLKVDELKAKAAELGIELPSNAKKAEIIELLQAHI"
     gene            8465..8809
                     /locus_tag="VHD02_001082"
     CDS             8465..8809
                     /locus_tag="VHD02_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497698.1"
                     /translation="MDETKQAIIDRVRVRLADETSLKEELLEELTQTAIDRINLKVGD
                     VVFNPLFNSIAVDVVVKMYRRMYFEGIDTEKADTISTKFIENVLAEYGEELASYKKDR
                     LAILNKKVVRFL"
     gene            8806..9117
                     /locus_tag="VHD02_001083"
     CDS             8806..9117
                     /locus_tag="VHD02_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497699.1"
                     /translation="MMFVNFDLVTSQKTGEKDRLGNDITKNVVKRVAKGRFTEWSADD
                     VSLYGRDLTSSARKLLTNQVSKAEAKQASHVVIDGSKYKVESVKDLGRWRLLVIKGYR
                     L"
     gene            9114..9509
                     /locus_tag="VHD02_001084"
     CDS             9114..9509
                     /locus_tag="VHD02_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497700.1"
                     /translation="MRMSMTGMPALEVKLRSMSEKRWDRVANKNLTEMFNRAARPPGT
                     PIGKNTKRHKSGELLRSRRLKKVNSSKDVITGNFGYIKDYAPHVEYGHRIVRNGKQVG
                     YANGTKYLFNNVKKQREIYRQDMLNELRR"
     gene            9511..9921
                     /locus_tag="VHD02_001085"
     CDS             9511..9921
                     /locus_tag="VHD02_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5072 family protein"
                     /protein_id="HEQ5497701.1"
                     /translation="MLKKLGLVDLHASIKQKIEDKTGLMAYDHVPEDMPSPFYFIEVV
                     DKRPEDTKVMWCEVFTVWIHAIAEAGKSKIAIYDMIEKLEEALTEELVLPEEIDILRQ
                     SEVGMQSLQEDETGEMHAIVAYEIKVSYGFKVKV"
     gene            9933..10439
                     /locus_tag="VHD02_001086"
     CDS             9933..10439
                     /locus_tag="VHD02_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEQ5497702.1"
                     /translation="MVAFDQNVYCNFDTSATKAIAGKDIILAIFDKTGANLLAISGQQ
                     GLTINRTADSIEVSSKDTKGGWKSKIPGMKEWSIDNDGLYVPSDETHKQLGEAFEKSE
                     FVCIKVINGKTKKGMFGGLASVSDYSLEAPYDDGMTYSISLEGNGALIDLSTLSSEDA
                     EKVTAMPS"
     gene            10452..10769
                     /locus_tag="VHD02_001087"
     CDS             10452..10769
                     /locus_tag="VHD02_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497703.1"
                     /translation="MDKELIIIDEKQYELKYNEKTIETVEALTGKAFMDVIVNNKGML
                     SLSMLRQYFANALYAVEGGRVSSEQGSNIFTKVLNTKGYAYVNMLVINTIQRDCPFFF
                     LGA"
     gene            10742..11200
                     /locus_tag="VHD02_001088"
     CDS             10742..11200
                     /locus_tag="VHD02_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497704.1"
                     /translation="MPFFLPRRLVDFEYLGGSGDSTDVEYDRLASQYHKDIDFAFYFV
                     NFGTTKSEFLELTRREKAFIRKAWEDKQVRESELMRNAVLNAVSNAMRKKSAKFVDLW
                     KRQQQPANMEIVEAHLEIINKNIADEGKYWVDLVYQANNMTKPSEGAENG"
     gene            11193..12998
                     /locus_tag="VHD02_001089"
     CDS             11193..12998
                     /locus_tag="VHD02_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ5497705.1"
                     /translation="MADYTLGVQITGDASKMQKAFEKAQEQAEKLKEKIKDSNNESGN
                     SFDGLTTKIDAISGKLSAYGDKISSIGKNMTTMFTLPAVAGITAVVKEYANLEQSIGG
                     VETLFKKSAKAVQKNANTAFRRAGVSANEYMEQVTSFSASLISSLGGDTAKAVGVADT
                     AMVDMSDNANKFGTNIGLIQNAYQGFAKGNYTMLDNLRLGFGGTKEEMQRLLDEAGKL
                     SGMEFDINNFSDIIQAIHIIQDNLGVTGTTAREASETISGSFSQAKASVIDFMGSLGG
                     DGSDVDAKMQAMIQSITVFADNVKEALGRIWDNLPLQEYQKNLLLIAVVGGPILVVVG
                     TIFGAIANIMTVVSGILTVLGMMTPIGWIIAAAIAAIVAAVVFWDDIVAEVGQKFELV
                     KIIGMAMWESFKDGVSSFGKSIQNVVGKIGGWFAEAWNAAADTATRIFTKVGDTISGI
                     FSGVVSGIKSLINGAIGGINKVIGITNKLPGVNIPKIPYLARGTSDWQGGFARINEGG
                     RGELVHMPNGATVVPHDVSMKYARESAKANSGVAYMDSNDEIAKSALKLANDAVKRPV
                     VLNINGREVAKTTGGDMRDYLNSRDMTLKRLRGEV"
     gene            12999..14483
                     /locus_tag="VHD02_001090"
     CDS             12999..14483
                     /locus_tag="VHD02_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEQ5497706.1"
                     /translation="MATMTFNDVDLSSLITIMSINRDIGNERQADTNDAPFIGLHIKK
                     IKTSAKIITVDFYLKDRTNEYALNQLKHKLAGIFNVNEQVKVTFSDEPDKYYLAIPIN
                     KISASDPIAWLSLVSLELLVPDGVAHSVSYKKITNYRQDGKKLLIDITNNGNVDAHPI
                     ITVKHNAENGYLAFVNKSSVFEVGDREIADAEIREKSVVAYDFKDERIVNALTAGKKN
                     VAILNDSTQVLDKNLLLNTVWGRKHLELEGSVAPSGNHAGTLTFDIPDGGSLYDYLWW
                     RQIFWVGTVSQLGFIKIMVSDENGHFLYGVETIKRTLGTKTEYNFLVTNGKGGYKITD
                     LRWTFDATHFETQNPFNEPRGWSDMTRADDTVSVFWWGSQNKRVFPELKGKKSKQVHI
                     AIGTIQGSPLVTHMYIDGFYYRKDKVPYEFNIPNAFGAGTEVVINAENDTVLVDNIPK
                     ANIIADGFFSFPKIPPGTSTLEVYSSSWSTANPDITLNFEERWL"
     gene            14484..17924
                     /locus_tag="VHD02_001091"
     CDS             14484..17924
                     /locus_tag="VHD02_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ5497707.1"
                     /translation="MLITIHNANLEKVAYIDNDKQDTLNYYDDKFSQYLNTANSTFEF
                     TVYKRGIKSDTVKEKAYLTLTERSFVSFKYNGKSYLFNVMTTDETDIEIRCYCENLNL
                     ELLNEYAGPYKAATQMSFVDYCNLFGILKNGAITIGTNETTDQKRTIEWTGQDTNLKR
                     LLSIANNFDAEIEFVTHLKNDSSLKSFVMNVYKKNDATNQGVGRRRDDIILQYGKNIE
                     SVRRKINKTGIYNAIRPSGKITTTTTTTTAKQGSVQTGSVLWSGGNLTYAGHVMQSSV
                     VNTILSLCSKYKLLPSGVFSQLYLESFWGDTPVGRADNNWGGITWTGATTRPSGINVS
                     QGQSRAEGGYYNHYASVDDYLKDYAYLLAEQGIYAVKGKLTIDEYTRGLFRVGGATYD
                     YAAAGYDHYAPLMRDIRAGINRNNNGAMDNVDNQFKNGGSTSQNTTQIAAKTKAVLAE
                     ANGLKGQRVGSGQCYALAAWYAMKLDGPGLNGGVTSFRGLIGAGAAAAQIGTDYNWGQ
                     FGWKVVQPNKVADLITGSIVNIRANAGSPVFTGAWGHTVVVKSLSGDTLTVLEQNYNN
                     VQTVQEHTYSASAYLSVVQTVCYPPEIVQGKRVEGTAQAEQPQSETTTISEEKEVLIN
                     PSLYREWKNEAGQVEFYVKNSMLYAPLSKSLYPSAFTGIETDDNWIRKDLDVDTESEE
                     KLISVALADLRKHCYPAVTYEVSGFIGDLDIGDTIKINDPEYTPSLILEARVSEQHIS
                     FTEPNQNKTVFDNYRALESKVSQGLIDRMNELAEAAKPYDLRLMTDNGNVFLNGEGRT
                     ILTAELWKGNKKFDASYQFKRDGQLVGAGLQLAVDAKDVPADRPLIITVEAYLNNELI
                     ASKQITFTNSLGEQGPAGRGIVSTEDYYLASPNRTGVTSATSGWTKTPQEITETNKYH
                     WYYHVDVYSDGTRKETTPAIIGVYGDKGSDGKQGKRGEMGPSGPPGALDKKQLQDINN
                     KIDGKADQNLTIEQINKLAELQSIANAELQAKASIDALASLQKQVQSAIAAMNASQKL
                     SEQDLITASQRAIKATNDILDLKEQWNFIDNYMSASEEGLIIGSKDGTSSVRVAKDRI
                     AFYSAGAEVASITGGMLKIDNGMFVATLQVGHFREEMYKVDGVDKHINVTRYYETIVG
                     "
     gene            17929..19791
                     /locus_tag="VHD02_001092"
     CDS             17929..19791
                     /locus_tag="VHD02_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497708.1"
                     /translation="MAEFWSNNDRGYRIKLTIDQVSQNTLANSSQVRVKLDLLNTTTT
                     FTQYSCSAYVDLNGQRIDWSDSPSVLRYNSTISLIDRTITVNHNADGTKSFGFIASFS
                     GSGGWSPGTLTVGSGTFTLTTIPRSSSVVVSSGTIGSAITININRQSSSFKHNLRYQW
                     GNKTGTIASDVDTSAVWTIPLDFASDIPNSTSGSGTIYVDTYNDSTLTGTQQVPFTVT
                     VPDSMKPTLSSISLSDAHTVAGNVVSSADYFIQVYSDIRVNFESASGSYGSTIKGYYA
                     EIVDKGQSTDQNGGTLGNMLYDGQITIRAKVIDSRGRESQPMDKTVTVLKYFPPALSF
                     DVARSGYGSDTLTVTRRASIAPLSVFGTQKNTMTLSFSVAELGSSYFSANNGSASGAW
                     ANVSSLVNSSANLYGAFSPTKSYTVKGILSDKFSRTEFTFDVGTESVVMSIAKNGIGF
                     QKIWEKGAIDAKGDAYISGKLFVNNTEVKPSFDKTEILNMVYPVGAIYMSTSSANPST
                     FIGGTWQRYAQGRTIVGVSENETEFNYVGKTGGAKTHTLTNEEMPSHSHGDKTISSGG
                     RPISSNAGWDNTNVGLYKSTDYNQINAFNKSSGGDQPHNNLQPYITTYIWLRTA"
     gene            19802..20149
                     /locus_tag="VHD02_001093"
     CDS             19802..20149
                     /locus_tag="VHD02_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEQ5497709.1"
                     /translation="MLVITSNYPEQMPDGSVSGAKVMLDGIDDHAGWHIPLTLLKEYL
                     DKPQSEVMTLCEKMIYQQLKPQKALNEKFAKLDATIEKGENMLKFATGILNQIIAAKE
                     EETTDDETVEKMD"
     gene            20299..20622
                     /locus_tag="VHD02_001094"
     CDS             20299..20622
                     /locus_tag="VHD02_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497710.1"
                     /translation="MTIDVTLFAQWCVALTAIIVFLKWVSAPVKHVLDNNKKAMEALE
                     SAIVKISDDLKDNNFKWTESKNHRDRLQKVQDQHEIRIGIVEDRLISHDEQLKTLWKV
                     KEEKK"
     gene            20622..20954
                     /locus_tag="VHD02_001095"
     CDS             20622..20954
                     /locus_tag="VHD02_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEQ5497711.1"
                     /translation="MEELIQVITGSALSILTIFAGIVVKLVKDYLLKKGGEKAVKIAE
                     IVARNAVEAVEQIAYDKDIKGIEKLTEAKIAVRDELSKHNVYLSDKQMEVFIEAAVKR
                     MNDSWKGQ"
     gene            20956..21720
                     /locus_tag="VHD02_001096"
     CDS             20956..21720
                     /locus_tag="VHD02_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ5497712.1"
                     /translation="MATVSEVISYWRGLADTGQGYDADLAWGWQCADVTNGTTTNFFG
                     VTLWGNAIDLLDSAKAQGLEVIYDAPGINPKAGDLFVMFTYGHPYGHTGIIIADSDGY
                     TIQTIEQNVDGYSDNNGDGINDQFQVGGPARYVTRAFSDGDGYIVGWIRPPYSDTSSE
                     KQSQSQAPSGFRKLKDEVGTFEVMVPALNVRREPSLNGEIVACYQYGMTGTYDSVYVG
                     DGYIWVSYVGASGMRNYMAVGDADGDYNVNPYCKFY"
     gene            21732..22334
                     /locus_tag="VHD02_001097"
     CDS             21732..22334
                     /locus_tag="VHD02_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497713.1"
                     /translation="MGYNSTNLKQVDGGDVIKQGDTSSLFSFNLLDENNNVIDLNGKQ
                     ATIYFTRNRKTYLTKTTDVIDNKVDFTINKILEIGTYYIEVHCDGYVFPSDDSVTLDV
                     RRSGQKYVVSTDLITDTTIQKLSADIEYLKSKVTQNQHLFEQVSPQTEWTITHNLIKY
                     PSVTIVDSAGNEVFGSVEYISTTKIIVRFSAPFAGKAILN"
     gene            22345..23118
                     /locus_tag="VHD02_001098"
     CDS             22345..23118
                     /locus_tag="VHD02_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497714.1"
                     /translation="MSTFLTNINLNKNELQNAVVQNLAKEPHEPKSGQFYFDSAANNL
                     KIYNGTSWVVLDKETAESIVDLINSGKSKIDKSKVDGLEEAINQASMTGQAIATALNE
                     DTGGVTVNQNKITGLTDKLAEIATNKASATRESQDALQEAKVYADQKVAGLVDSASED
                     YNTFKELETFIKQNAKGLAAISNVAKKFSKTFGDARAKEFTITHNLKTRDVNVTIRET
                     NFPYAIVYADIKVTDENSILIATGAEVLTQNQYTVTITG"
     gene            23128..23349
                     /locus_tag="VHD02_001099"
     CDS             23128..23349
                     /locus_tag="VHD02_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497715.1"
                     /translation="MRYIGESQLSKDLVTKDYVDNKVTNLASKDDIVNLGKIKDSQTG
                     AFLEVKIVPNGQVPYDTTGMIVFERSGGK"
     gene            23349..24008
                     /locus_tag="VHD02_001100"
     CDS             23349..24008
                     /locus_tag="VHD02_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497716.1"
                     /translation="MSVQEIPDKIFYRLIEQTVEAKLVWDKDTQIGSYYISIHDKYSF
                     ESGRDVNIYKILSSTQADVYKANKYNYQSSSKPDTSVPQPKPPEQPPLKNKSLYQSIT
                     IDGLPIARVICNNQVIWSQSAEPQEVYNITAYVDASVSKLNDSAIIDYNRDFAAYGVN
                     WSRVDRVAVEVDDKIVAKLQAPVYDFGVRGGTTMSVEKWDYPSNTIDKYKNPTFKILQ
                     H"
     gene            complement(24130..24837)
                     /gene="speA"
                     /locus_tag="VHD02_001101"
     CDS             complement(24130..24837)
                     /gene="speA"
                     /locus_tag="VHD02_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeA"
                     /protein_id="HEQ5497717.1"
                     /translation="MTFLGLTISQEVFAQQDPDPSQLHRSSLVKNLQNIYFLYEGDPV
                     THENVKSVDQLLSHDLIYNVSGPNYDKLKTELKNQEMATLFKDKNVDIYSVEYYHLCY
                     LCENAERSACIYGGVTNHEGNHLEIPKKIVVKVSIDGIQSLSFDIETNKKMVTAQELD
                     YKVRKYLTDNKQLYTNGPSKYETGYIKFIPKNKESFWFDFFPEPEFTQSKYLMIYKDN
                     ETLDSNTSQIEVYLTTK"
BASE COUNT         8308 a         4403 c         5375 g         6763 t
ORIGIN      
        1 gactctttaa caagacaagt taaaaacatt tattttttgc aaaatacaaa agacgacatg
       61 ctgagtttta gtgatattaa cgttatgcca agaactcaag atattgagcg agaatttaat
      121 gtcaaagagt ggattggtga tgcgatagtg catgtagaga gtaagttaaa tgcgaggtaa
      181 aaatgaacat tgaagaagcg aaaagagcga taagagaact agaggcgttt aattatttcg
      241 attttaaagg taatttaatt aaacgaattg atgtattagg tatcatcgac caactcggcc
      301 aaccaaaagt ggtagtgccg aagccggtat ttgaaatgat taagtttaga aaagaacacg
      361 accaggattt aacaacaact tttgttaata tacatgctta tccagacaat gttttccatt
      421 gggtggcaaa taacggtgat ttatttgccc aagcatggct agcttatcca aatatcacca
      481 tcgaaaaaga gaagctatat acagtggaga taccaaatcc gaacagggaa ggcagaggtc
      541 atgccaagtt tgtgcttgaa agacaaggta ataaagtttt tttggtaaaa cgaaaatcaa
      601 aggattgcta ctacaataaa aacagcaatc acctcactga atccgaaatt cgtaaagatt
      661 tcgactgggc atggcgagaa ggatttgcgg aggaggtgac ggaatgaaag aaaaaacaat
      721 ttttatatca aaaaaatatg caaatgactt taacaatgac aaatataatt tgtcctctgg
      781 ctattatttt aaaaatggtg aacaacatga tattgctatt gttaaatatg gcgaacaaga
      841 ttatttaaaa aatactgatt tagcatatgt tgtatgcgat aaaatcgttg atggagaatc
      901 tataggcttc gtttatcacg gcgaatatga aacttggcgt tttaaactat taaacacgga
      961 agcaaattaa attcccgcgc aagcgctcaa gagcctgcga tggctctgtg ggggtggacc
     1021 gaaattaaaa aatagaaacg agaacctcct tacaccaaaa caaatctaaa gcgggttatc
     1081 ggtcatccgt gattatccaa ggcgtcgcta atgctttaac acgacatcgt gcgcctgtgt
     1141 caaaaaacaa aggaaagaga ggacttttct ccacaaaaca aaaagacgtc catacggaac
     1201 gcccccttgg ttaaatttaa gcttaaataa attataccac attggggggg ctttcatgac
     1261 gttttttcca gagattgata tccaaaaaac aaaatctaat gccaagcgta aattgagaga
     1321 gtatccacgt tggcgaagga tagctaatga tgtagatact caaaaagtga cagccactta
     1381 ttcctttgag ccaagacaac cgtatggaac gccaagcaag cctgttgaga gactagcgct
     1441 caaccgtgtg tcagcagaac aagagctgga tgcgattgag caagccgtca gtatgatact
     1501 agagccagag agacgcagga ttttgtatga caaatactta gcgccttata aaaaggcaga
     1561 taaggttatt tatacagaat tgtgtatgtc agagagcttt tactatgata cacttgacat
     1621 tgctttgtta gcttttgcag agctgtacag agagggtgtg ttgcttgctg agaaaggagt
     1681 ttttagctag tttttataca gtaataagat agtttataca tatttttaca tgttattata
     1741 gtactatcaa aataacaaga agagataacc ttttaatcac tgactatttt atttagtcgc
     1801 caactttaac tacaatcaaa cttgttattt tgtagcctga tggcggtaca gcgagttgag
     1861 acgacaactg ggtatgcagg ttcgattcct gccgtctcaa tcagcgccca gaagaggcgc
     1921 gacccagtat cccgaaaggc agtcacgcac tgatatactg gtttttactt ggttgatagt
     1981 catattccta ccaatgacga ttgataaaaa agatacatag atcggctgta tagtctacgt
     2041 gtacgtattc acaacagatt gactcatttg attaaccgca agtaaaacaa gggtcgcaac
     2101 cttgcctgtg gttagtaggc ttaatggtcg tttatgcacg ttcgattcgt gcaaagcctg
     2161 tttgataaat agaagtgtcc cagaatgggg taggcaatag gcttagcatt catttgctat
     2221 ttatctatgg ttaaccaatt agtcatcaca ttgtggtgac ttttttattt ccgaaaattt
     2281 ccgaaaggag gaacatattt tggcaagagc aaataaggca aaaatgcttg atgctattga
     2341 gtttaaatta ttaaatccaa ccgctaatca agcacaagtt gcggaaataa tcggtatcac
     2401 agacagacag ctacgaaatt gggaaaatga cccagaagat aaatcgggtt ggaataaatt
     2461 aaacgatgaa atcgcagaaa aagcttttgg caaatatgct ctttctgcga ttggaacgct
     2521 tgttaatcta agcaaaaacg caaaatcaga attggtaaga ctacaagctt ctcaagatat
     2581 ccttgaccga gcaggttata aaccgacaga caagcaggaa atcagcatag acgagcctat
     2641 cgtacttgct aattcatggg tgcaagacaa tggtagttga tttagctgat attatcccaa
     2701 ttggttttaa gcctgtcgtg caagcgactt ggaatcctca aatactaaat atcgcttgca
     2761 aaggcggacg tggttctggt aaatcatcta atattgcttt tattatctcg aggttaataa
     2821 tacagtatcc tgtcaatgcg gtttgtatcc gtaaaacaga taatactctg gagcagtctg
     2881 tttatgagca aattaaatgg gctatatctg agcaagggct agagcgttac tttaaattta
     2941 acaaatcacc tctaaggata acgtacatcc ctagaggtaa ttacattgtc tttagaggag
     3001 cacaaaatcc tgaacgtatc aagtccttaa aagatagtcg ttttcctttt gctatcggtt
     3061 ggatcgagga gcttgcggaa tttaaaaccg aggacgaagt caaaactatc actaactccc
     3121 tattgcgtgg ggaactcggt gatggtcttt tttataaatt tttttataca tacaatccac
     3181 ctaaacgtaa acagtcttgg gttaacaaaa aatatgagag ccaatttcag ccgtctaata
     3241 cgtttgtaca cgcatctacg tataaagata atccttttat tgccaaagag tttatagccg
     3301 aggcagaagc cacgagagag cgttcagaac gtcgttatcg ttgggagtat ttaggagagg
     3361 ccattggttc gggtgttgtt ccatttgaca acttacgctt cgaacgtatt actgacgaac
     3421 aagtagccga tttcgataat atccgtaacg gtattgacta tggttatgca actgacccgc
     3481 ttgcttttgt acgttggcat tatgataaaa agaaaaatgg tatctatgct attgacgagt
     3541 attatggtca gaagatatca aacaggcagc tggctaagtg gttgacgact aaaggatatc
     3601 agagtgatga gatgtttgct gaaagtgctg agcctaaaag taatgctgag cttaaaaacg
     3661 agtttggcat caaacggata aaaggcgtta aaaaaggacc agattctgtc gagtttgggg
     3721 agcgctggtt agatgactta gattttatct gtatagaccc aaaacggaca ccaaatatcg
     3781 ctcgagagtt tgaaaatatc gactatcaag tcgatagaga tggcaaccct aaacctcgtt
     3841 tagaagacaa ggataaccat gcgatagatg cgacaaggta tgctatgtca gacgacatga
     3901 gagcgactaa aaccatcgtc aaaacattta aaggaggtat ctagtgccag aattatttat
     3961 tgtgccatca gatacagaga tgacaaaaga tttgttaagc gagttaatcc aaaaacacag
     4021 gtcgtttaac cttagttatt cagcgtataa gcagctatat gagggtgatc atgctatttt
     4081 gcaacaaaaa caaaaagagc aatacaagcc tgataatcgc ttggttgtta attttgcaaa
     4141 atacatcgtt gatacattta acggctattt tatcggtgtg ccagtccaaa cgagccacga
     4201 gaataaacaa gtcagcaatt atttagagtt attagacgga tacaatgatc aagacgataa
     4261 caacgcagaa ctatcaaaga tttgcagtat ttacggacat ggctatgaat tagtttttaa
     4321 tgacgagaat gcagaggttg gaattactta tctgacgcct cttgaaggat ttattgtcta
     4381 tgacgactct attagacaaa agccgttatt tgctgtgcgc tatttttata ataaggacgg
     4441 tgttttagag gggtcttatt cagacgccag caaaatcact tattttaaag atggcgaaaa
     4501 agaccttgaa atcggggaga gcgatccaca tccgtttgat ggtgtgccaa tgattgagta
     4561 tgtcgaaaac gaagagcggc aaagcttgct agctagcgtt gtgacattaa ttaatgcgtt
     4621 taataaagct atctccgaaa aagccaatga cgttgagtat ttcgcagacg cttatcttaa
     4681 aattttgggt gctgagctag acgacgagac attaaagtct cttagagaca cccgcattat
     4741 caatctaaaa gacaccgatg cacagcaatt aactgttgag tttttacaaa agccagatgc
     4801 cgatgcaacg caagagcatt tgctcgaccg actagagaac ttaattttta ggactgctat
     4861 ggttgctaat atcagtgacg aatcgttcgg tacagctagc ggcatcgctt tacgttatcg
     4921 tttgcaagct atggataact tagctaagac aaaagagcgt aagtttatga gcgggatgaa
     4981 ccgcagatat aagcttattg caagctatcc tacgtctaag ataggaccta aagattggat
     5041 tggcattaag tataaattta ctcgcaatct accagcaaat ctcttagagg agtctcaaat
     5101 tgcaggcaat ttagcgggga ttgtctcgga agaaacacag gtcggtgtgc tatctattgt
     5161 ggaaaaccca caaaaagaaa tcgaaaagaa aaatagcgac aggccaacct taattagtcg
     5221 aaaagcagga gggctaaatg gccaagacac cacaacagta ttggaatgag cgccagaagc
     5281 aactatgggc taatttagag acgtcagagc gcgctttaca gactaagctt agcaaatatt
     5341 acgctgatga agcaaagtcc ttagaaaagg aaataggggc ttattttagc aagtatggaa
     5401 aagacaacgt tattgaatat cgaaatcttt tgcagcagtt atctaaagct gataaagact
     5461 tgctttatcg agattgtgag cgatttgcta aaaaatatcc acaacgtgct gatttaatgc
     5521 ctgtcaggac ctctatttat aaattagata gactgcaagg actagaatta agcattaaaa
     5581 tacaacagtt ggaaataggt gctatcgaag aggcggaatt gactaagcat ttaacgacag
     5641 cctttaaaaa aggttatcaa gaaaccgcta aaacaattgg tttccaagcg gataaagtat
     5701 ccgctgagct gtttgttaac aatgattgga caggcaaagg tgattttagc tcaagtattt
     5761 ggtctaacaa agataagctt gtcgattatc taacaaatga ctttaagacg gcaattatca
     5821 ggggagatag ctttgataaa gtcgttaaac agatgtcaga gcgttttact gtacgttcgc
     5881 agtcggatat tacacggctg atcatgacag aagggactta tgtcaataat caagcgatga
     5941 tggcaccgtt tgaggatagt gacgactttg aggagtacga atttgtctcg gttttagata
     6001 gctctactag ctcaatttgc agaggattag acggtcataa atttaaactt aaagatagac
     6061 aggttggggt taacttcccg cccatgcacg ctaactgtcg cagcacattt gctatggtta
     6121 ttccagatga ttatttagag aggtaaaaat ggatgaataa acgcattaag aaaaaacgta
     6181 aattagaaac agctattgtg atgattgttg cagagaacgc catgcaagca gaagcactca
     6241 ggaatcaaaa caaagaaatc atggagttaa aatcaatcgt tcaacgaaac gctctggcaa
     6301 caaacgaaga gttagcgact gttaaagctg ctactttaga taaccaatca gccatcaagt
     6361 caatcggcga tgatgttgac tacatcaagc aaaattacaa acggaagtgg aggaagaaat
     6421 aatatggtgt tagagcaagc tgaactagaa atggttagaa atcaacgtct ggttatgatt
     6481 aaccctgaaa tggtaactat cacaaaaaca gaatatgatc tgttgattga aataaaaaac
     6541 aaatttttag agtcgtagca atacggcttt ttattatgtc ctgtcgcatg acaaaaaact
     6601 aggcaaataa aaatctaaag caatgcgctg gggcttttga gcaatggcgt ggggcaggag
     6661 gaaaaaatgg aaaacgaaga aatcctagaa caatctggtg cacaagaaga agctaaggag
     6721 caaacgtttg acgatatttt gtcagaccct aaaaagcaag ctgaatttga caagcgtgtg
     6781 gctaaggcta ttgatactgc acgaaataaa tgggtggctg agactgaaga aaaagaaaac
     6841 gaagctaaga gacttgcaaa aatgaatgct gaacaaaaag cacagcacga aaaagcaaaa
     6901 cttgaagcac gcattgcaga gctagaagct gaacgtacat tgtcagaaat gaagagtgct
     6961 gctcgcacaa tgctatcaga agctaatatc aacatctctg acgcactttt atcgcaattg
     7021 gtatctactg atgcagataa aaccaaaaat gcagtagaag cgttttctga ggcattttcc
     7081 gaagcaatcg aaaaagaagt caaagaacgc cttaaatcgc caacacctaa aaaatcaaat
     7141 ggcaatactg gactgacaaa agaacaaatt ttagcagtca aagatactgc ggaacgacaa
     7201 aaactcattg ccgaaaatat cggcctattc caataaaaca ggagaaatta acacatggta
     7261 acatcacgta cttacccaga agaaaatcta atcaaatcaa cagaccttaa ataccctatc
     7321 actattgatg tcacaaacaa attccaagaa aatatctcga aattacttga aatgctcggg
     7381 gttactcgta aaatctcggt atctgagggt atgaccctta agacatacgc tggttatgat
     7441 gtcacattag cagaaggtaa cgtcccagaa ggcgaagtta tcccgttatc aaaagtagag
     7501 cgcaaaatac actctgagaa aaaaatcgaa cttaaaaaat atcgtaaagc aacaaccggc
     7561 gaagacatcc aaatgtatgg ctctaacgaa gctgtaacaa atactgacaa cgcacttgtt
     7621 cgtcagttgc aaaagaaaat ccgcacagac tttgtgactg ctcttaaaac aggtacagga
     7681 acacaagatg ctcttggtgc aggattgcaa ggggctttag cttctgcttg gggtaaactg
     7741 caagtgctct ttgaagatta tggttcagag cgtgcgattg tgtttgctaa tagccttgat
     7801 gtcgctgaat acatcgctaa agctggcatc actactcaaa ccgcattcgg attgacttac
     7861 cttgtcgatt ttacagggac ggtcattatc tcgacaaacg atgtcacaaa aggggaaatc
     7921 tgggcgacag taccagaaaa tatcatcttt gcttacatca atccaaataa ttctgaatta
     7981 gctaaagaat ttaatcttta cggtgatcca accggctaca ttggaatgaa tcacttccaa
     8041 gaaaatacaa cgcttactat ccaaacgttg cttgtatctg gtatgttgat gtacccagaa
     8101 cgtattgacg ggattgttaa agtaactctt acaccaggcg tctaatgggg gtagcttatg
     8161 gcttatagag taaaagcacg tttttttgat ttgcttgaca atagcttttt gtacgaggtt
     8221 ggcgacagct ttcctcgcaa aggctatgag ccaagcaaag agcgcttaga aagcttgtta
     8281 tctagcaaca atacagaaca taaaccattt attgaggtgt caggtaatgg caatagctta
     8341 gatggtttaa aagtggatga gttaaaagct aaagcggcag agctaggaat cgaactccca
     8401 agtaatgcta aaaaagctga aatcatcgaa ttactgcaag cacacatcta gaaagtaggt
     8461 tgacatggac gaaacaaaac aagcaattat tgatcgtgtc cgtgtccgtt tagcagacga
     8521 aacgtcttta aaagaggagt tattggagga gttaacgcaa acggctatcg accgtattaa
     8581 tttaaaagtc ggcgatgtcg tttttaaccc tctttttaat tccatcgctg ttgatgttgt
     8641 tgtcaaaatg tatcgtcgta tgtattttga aggaattgac acggaaaaag cagacacaat
     8701 atcaactaaa tttattgaaa acgttttggc agaatatggc gaggagttag cgtcatataa
     8761 aaaggaccgt ctagccatct tgaataaaaa ggtggtgcgg tttttatgat gtttgttaat
     8821 tttgacttag taacatcgca aaaaacggga gaaaaagaca gactcggcaa cgacatcacg
     8881 aaaaatgttg tcaaaagagt tgctaaaggt cgttttactg aatggtcggc tgatgacgtg
     8941 tccttatacg gtcgagattt aacgtctagc gcacgcaaat tgctgactaa tcaagttagc
     9001 aaggcggaag ccaaacaagc gtcacacgtt gtaatagacg gctcaaaata caaagttgaa
     9061 tccgttaaag atcttggtag atggagacta ctcgtcatta aagggtatcg cttatgagga
     9121 tgtcaatgac aggtatgcca gccttagagg taaaactaag gtcaatgagc gagaagcgct
     9181 gggatagggt tgccaacaaa aaccttacag agatgtttaa cagagcagca agacctcctg
     9241 gtacaccgat tggtaaaaac actaaaaggc ataagtctgg cgagttgttg cgctctagac
     9301 gtctcaaaaa ggtaaactca tcaaaagatg ttatcacagg taactttggt tatatcaagg
     9361 actatgcacc tcatgtcgag tatggacaca ggattgtccg caacggcaaa caggttggct
     9421 atgcaaatgg tacgaaatac ctgtttaata acgtcaaaaa acagcgtgaa atttataggc
     9481 aagacatgct aaacgaattg aggagatgac atgttaaaaa agttgggact ggttgattta
     9541 cacgcctcaa ttaaacaaaa aatcgaagat aagacagggt tgatggctta tgatcatgtc
     9601 ccagaagaca tgccatcgcc cttttatttt atcgaggttg tggataaacg gccagaagac
     9661 acaaaggtca tgtggtgcga agtgtttacg gtttggatac atgccatagc ggaagctgga
     9721 aaaagcaaaa tcgctattta tgacatgatt gaaaaactcg aagaagcgct cacagaagaa
     9781 ttagttttgc cagaagaaat cgacattttg agacaatcag aagtcggcat gcagtcctta
     9841 caagaggatg agactggcga aatgcatgca attgtagcct acgaaatcaa ggtttcgtac
     9901 ggttttaaag taaaagttta aaaggagaaa taatggtggc atttgaccaa aatgtttatt
     9961 gtaattttga cacatcagca acaaaagcta ttgcaggtaa agacattatc ttggcaatct
    10021 ttgataaaac tggtgcaaat ttacttgcta ttagtggtca gcaagggtta acaatcaacc
    10081 gtacggctga ctcaattgaa gtatcgtcta aagatacaaa gggtggatgg aaatctaaaa
    10141 tccctggcat gaaagaatgg tctatcgata atgatggtct ttacgtgccg tcagacgaga
    10201 ctcacaaaca attgggcgaa gcttttgaaa agagcgagtt tgtttgtatt aaagttatta
    10261 acggtaaaac caaaaaaggc atgtttggag ggcttgcgtc ggtaagtgat tatagcctag
    10321 aagctcctta cgatgatggt atgacatact caatcagtct cgaaggcaat ggagcattga
    10381 ttgatttatc aacattatca tcagaagatg ctgaaaaagt aacggctatg cctagttaat
    10441 gagaggaatg aatggataaa gaattaatta ttattgacga aaaacaatac gaactaaagt
    10501 acaacgaaaa aaccattgaa accgtcgaag ctttgacagg taaagcgttt atggatgtta
    10561 ttgtgaataa caaaggtatg ttgtcgctat ctatgttgcg tcagtacttt gctaacgctc
    10621 tttatgcggt agaaggcggt cgtgtgtcta gcgagcaagg ctctaatatt tttactaaag
    10681 tgttaaatac aaaaggttat gcgtatgtaa atatgctagt gatcaacaca atccagcgtg
    10741 attgcccttt tttcttcctc ggcgcttagt cgactttgag tatctcggcg ggtctggcga
    10801 tagcacagat gtagagtatg acagattagc aagtcagtat cacaaagata ttgactttgc
    10861 tttttatttt gtcaattttg ggactacaaa atccgaattt ttggaattaa ccagacgaga
    10921 aaaagccttt atcagaaaag cttgggaaga taaacaggtg cgagaaagtg agcttatgcg
    10981 caacgctgtt ctaaatgcag tcagtaacgc tatgcgcaaa aaaagcgcta aatttgttga
    11041 tttgtggaaa cgtcagcaac agcctgcgaa catggaaatc gtcgaagcgc atttggaaat
    11101 cattaataaa aacatcgctg atgaaggcaa atattgggtt gatttagtct atcaagctaa
    11161 caacatgaca aaaccgagcg agggggcaga aaatggctga ttatacttta ggagtacaaa
    11221 taacaggtga cgcctctaaa atgcaaaaag cgtttgaaaa agcgcaagaa caagctgaaa
    11281 aattaaagga aaaaattaaa gactcaaaca acgaatctgg caattctttt gatggtttga
    11341 ccactaaaat tgacgcaata tctggcaagc tgtcagctta cggagataaa ataagctcta
    11401 ttggcaaaaa catgacaacc atgtttacat taccagcggt tgctggtata acggcagttg
    11461 ttaaagagta tgcaaatctg gaacagtcaa ttggtggtgt tgagacttta tttaaaaagt
    11521 ctgccaaagc tgtgcaaaaa aatgcaaata cagcgtttag acgagctggt gtatctgcta
    11581 atgagtacat ggagcaagta actagctttt ctgctagtct aatttcatct cttggcggag
    11641 atacagcaaa ggctgtcggt gttgctgata ctgccatggt cgacatgtct gacaacgcta
    11701 ataaatttgg tacaaacatc gggttaatcc aaaatgccta tcaaggcttt gctaaaggca
    11761 attacacgat gttagataac cttagattgg gttttggtgg gacgaaagag gagatgcaaa
    11821 gattgctgga cgaagctggt aagctaagcg gcatggaatt tgacatcaat aacttttcgg
    11881 acattataca agcaatccac attatccaag ataatttagg cgtaacagga acgacagcta
    11941 gagaagcgtc agaaactatt agcggctcat tttcgcaagc aaaagcgtca gtaatcgatt
    12001 tcatgggttc tcttggcgga gatggcagtg acgtagatgc aaaaatgcaa gcaatgatac
    12061 agtcgattac tgtgtttgca gataatgtca aagaggcttt ggggcgtatt tgggacaatt
    12121 tgcctttaca agagtatcaa aaaaacctct tgcttatagc tgtcgttggc ggacctatcc
    12181 ttgttgtggt cgggactatt tttggagcta ttgctaatat catgactgtt gttagcggta
    12241 ttttaaccgt tttaggcatg atgacgccaa taggatggat catagcagca gccatagcgg
    12301 ctattgtcgc agctgtcgtt ttttgggacg atattgttgc agaggtcggc caaaaatttg
    12361 agttagtcaa aataatcggc atggcgatgt gggaaagttt taaagatggt gtatctagtt
    12421 ttggcaagtc aatccaaaat gtcgttggca aaattggcgg atggttcgcc gaagcgtgga
    12481 atgctgcagc cgacacagcc actagaattt ttactaaggt tggggatacg attagtggca
    12541 tctttagcgg cgttgtttct ggcattaaaa gtttgatcaa cggagcaatc ggtggcatca
    12601 ataaggttat tggcattacc aataaactgc ccggtgtcaa cataccaaaa atcccatatc
    12661 tcgccagagg tacatctgac tggcaaggcg gttttgctcg tattaacgag ggcggtcgtg
    12721 gagaattagt tcatatgcca aacggggcaa ccgttgtccc gcacgatgtc agcatgaaat
    12781 acgctcgaga atctgctaaa gctaattctg gtgttgctta tatggatagt aatgatgaga
    12841 ttgccaaaag tgctttgaaa ctagctaacg atgctgttaa acgccctgtt gtgttaaata
    12901 tcaacggccg tgaggtcgct aaaacgacag gcggagatat gagagattat ctaaatagtc
    12961 gagatatgac attaaaacga ttgagaggtg aggtttagat ggctacaatg acttttaatg
    13021 atgtggactt atctagcctc atcactatca tgtctattaa tcgcgacatc ggaaacgagc
    13081 gacaagcaga tactaatgat gctccgttca tcggattgca cattaaaaag attaaaacta
    13141 gcgcaaaaat cattacagtt gacttttatt taaaagacag gacaaacgag tacgcattaa
    13201 accagctaaa acataaacta gctggtattt ttaatgttaa cgagcaagtc aaagtaactt
    13261 ttagcgacga accagataaa tattatctag ctataccgat aaataaaata tcggctagcg
    13321 acccaatcgc ttggctaagc cttgtgagct tggaattact tgttcctgac ggcgtggccc
    13381 attctgtgtc atacaaaaaa atcacgaatt acagacaaga tggcaagaag ctactgattg
    13441 acattacaaa caacggtaat gttgatgcgc atccgatcat tactgttaag cacaacgccg
    13501 agaatggtta tttagctttt gtaaataaat catccgtttt cgaggtcgga gacagggaaa
    13561 tagcagacgc tgaaatccgt gaaaagtcgg tcgtcgctta tgattttaaa gacgaaagaa
    13621 tcgtcaatgc cttaacagca ggtaaaaaga atgttgctat cttaaatgac tcgacacaag
    13681 ttcttgataa aaatttatta ctaaataccg tctggggaag aaaacatctc gagttagaag
    13741 gaagtgttgc gccaagtggc aaccatgctg gaacgttaac gtttgatatt cctgacggag
    13801 gtagcttata tgactatctt tggtggagac aaattttttg ggttgggact gtttcccagc
    13861 ttggctttat caaaatcatg gtatcagacg aaaacggaca ttttttatat ggagtagaaa
    13921 ctataaaaag aacgttagga acaaaaacag agtataactt tttggtcact aatggaaaag
    13981 gtggatataa aataactgat ttacgttgga cttttgatgc tactcacttc gaaacacaaa
    14041 acccctttaa cgaacccaga ggttggtctg atatgacaag ggctgatgac actgtatctg
    14101 ttttttggtg gggttctcaa aacaagcgag tattcccaga attaaaaggc aaaaaatcta
    14161 aacaggtaca catcgcaatc ggcactattc aaggcagtcc tttagtaact cacatgtata
    14221 ttgatggatt ttattatcgc aaggacaaag taccgtacga atttaatatt ccgaatgctt
    14281 tcggggcagg aacagaagtc gttattaatg ccgaaaatga cacagtttta gttgataaca
    14341 ttccaaaagc taacatcatc gccgatggct tttttagttt tcctaaaatt cctcctggaa
    14401 catcaacatt agaggtttat tcctctagtt ggtctaccgc caacccagat attacgctaa
    14461 attttgaaga aaggtggcta taattgctta taacaatcca caacgcaaat ttagaaaaag
    14521 ttgcttatat tgataacgat aagcaagaca ccttgaatta ttatgacgac aaattttcac
    14581 agtatttaaa tacggccaat tcgacatttg agtttacggt ttataagcga ggaattaagt
    14641 ccgacacagt caaagagaaa gcctatctga cacttacaga aaggtctttt gtgtcgttta
    14701 aatataatgg caagtcttat ctgtttaacg taatgacaac agacgagaca gacatcgaga
    14761 tacgttgtta ctgcgagaac ctcaatctcg agttgctcaa tgagtatgca ggaccttata
    14821 aagcggcaac tcagatgtct tttgtcgatt attgtaattt gtttggcatc cttaaaaacg
    14881 gtgctattac cattggcact aatgagacaa ccgaccaaaa acgcacaatc gaatggactg
    14941 gtcaagatac caatcttaaa cgcttgttat ctatcgccaa taattttgat gcggaaatag
    15001 aatttgtaac gcatcttaaa aacgattcta gtcttaaatc gtttgtcatg aatgtctaca
    15061 agaagaacga cgctactaat cagggcgttg gtcgtagacg agatgatatt attttgcaat
    15121 atggcaaaaa tatcgagagt gtcagacgta agattaataa aacaggcatt tacaatgcta
    15181 taagaccaag cggtaaaata acaacgacta caaccacaac gactgctaaa caaggctctg
    15241 tgcaaacagg gtctgttttg tggtctggcg gtaacttgac ttatgcaggt catgtaatgc
    15301 aatcatctgt tgttaacact attttaagcc tatgtagcaa atacaaactt ttaccatctg
    15361 gtgtttttag ccagctatac cttgaatcgt tttggggaga taccccagtc ggaagagccg
    15421 ataataactg gggtggtatc acttggactg gcgcaacaac taggccaagc ggaataaatg
    15481 tctcccaagg gcagtctcgt gctgaaggtg gttattataa ccattacgcc agtgttgatg
    15541 actacttgaa agattacgct tacctcttgg ccgagcaagg catttatgcc gtaaaaggta
    15601 agctaaccat tgatgagtac acaagaggtc tgtttagggt cggtggcgca acatatgatt
    15661 atgctgcagc tggatatgat cattatgcac ctttgatgcg agacatcaga gcaggtatta
    15721 accgtaataa taacggcgct atggataacg tcgataacca atttaaaaat ggtggctcga
    15781 ctagtcaaaa cactactcag atagctgcta aaacaaaagc ggtacttgcg gaagcaaatg
    15841 gactgaaagg tcaacgagta ggctctggtc agtgctatgc gttagctgct tggtacgcca
    15901 tgaaattaga tggtccaggt ctgaacggtg gtgtaactag ttttagaggg cttattggtg
    15961 ctggtgctgc cgctgctcag attggtacgg attacaactg gggtcagttt ggctggaaag
    16021 ttgtacaacc gaataaagtc gcagacttaa tcacaggctc gattgttaac atcagagcaa
    16081 acgctggcag tcctgttttt acaggcgctt gggggcatac tgttgttgtt aaatctctat
    16141 ctggagatac actcacagta ttagagcaaa actataacaa cgtgcaaaca gtccaagagc
    16201 atacatatag cgctagcgct tatttatcag ttgtacagac agtctgttat ccgccagaaa
    16261 tcgttcaagg aaaacgcgtc gaaggtactg cacaagcaga acaaccgcaa tctgaaacga
    16321 ccacaatatc tgaagaaaaa gaggtcttaa ttaatccatc actttatcgt gagtggaaaa
    16381 acgaagcggg acaagtcgag ttttacgtta aaaacagtat gctctacgca cctctgtcaa
    16441 aatctcttta cccgtcagcg tttactggca tcgagacgga cgataactgg atacgaaaag
    16501 acttagatgt tgacacagag agcgaggaaa agcttatctc tgttgctctc gcagacctga
    16561 gaaaacattg ttatccagcg gtgacttatg aagtatctgg ttttattggt gatttagata
    16621 ttggtgacac tatcaaaatc aatgacccag aatatacccc aagtttaatt ttagaagcaa
    16681 gggttagcga gcaacacatc tcgtttacag agcctaatca aaataagaca gtatttgata
    16741 attacagagc tttagagagc aaagtctcac aaggtttaat tgatcgcatg aatgaactag
    16801 cagaagctgc taaaccttac gacttgcggt taatgacaga taacggaaat gtgtttttaa
    16861 atggcgaagg tcgtacgatt ttaaccgctg aactttggaa aggtaacaaa aagtttgatg
    16921 caagctatca atttaaacgt gatggtcaat tagtcggcgc tggattgcag ttggcagttg
    16981 acgctaagga tgtaccagct gatagacctc taatcattac tgttgaggct tatttaaata
    17041 atgagctgat tgcaagtaaa cagattacgt ttactaactc gcttggagaa caaggaccag
    17101 ctggacgtgg gattgtctct acagaggact attacttagc gtcaccaaat cgtacaggtg
    17161 tcacatctgc aacatctggt tggactaaga cgcctcagga aattactgag actaataaat
    17221 atcattggta ttatcacgtt gatgtttatt cagatggcac tcgaaaagag actacaccgg
    17281 ctattattgg tgtttacggc gataaaggtt cagatggcaa gcaaggcaaa cgtggggaga
    17341 tgggaccatc aggacctcct ggcgctttag ataaaaagca actacaagac atcaataata
    17401 aaattgatgg caaagcagac cagaatttga ccatcgagca aatcaataaa ttagccgagt
    17461 tacaatctat tgccaacgct gagttacaag caaaggctag catcgacgct ttagctagct
    17521 tacaaaagca agtacagtct gcaatagcgg caatgaatgc tagccaaaag ttatcagagc
    17581 aagaccttat tacagctagt caacgggcta tcaaagcaac caacgacatc ttagacttaa
    17641 aagagcaatg gaattttatt gacaattata tgtcagcgtc tgaagaggga cttattatcg
    17701 gctctaaaga cggtacaagt tccgtgcgtg ttgccaaaga ccgtattgcc ttttactcag
    17761 ctggcgcaga agtcgcttcg attactggtg gtatgctcaa aattgataat ggtatgtttg
    17821 tggctacttt gcaagttgga cattttcgcg aggagatgta caaagttgat ggggtagata
    17881 aacacataaa tgttacaaga tattacgaaa cgattgtggg gtgattaatt ggcagagttt
    17941 tggtcaaaca atgatagagg ttaccgcatc aaacttacga ttgatcaagt atcccaaaat
    18001 acattagcaa acagtagcca agtgagggtt aaacttgatt tgctaaatac aacaacaact
    18061 tttactcaat atagttgttc tgcatatgtt gacttaaacg gtcagcggat agactggtca
    18121 gactcaccat cagtacttag atataactca acaatttctc tgatcgatag aacaataaca
    18181 gttaatcata atgctgatgg tactaaatca tttggattta tcgctagctt tagcggctct
    18241 ggtggatggt ctccaggtac tttgactgtt ggtagtggta cttttacact tactaccatc
    18301 ccacgctcaa gctccgttgt tgtttcttct ggtactattg gcagtgccat cactatcaac
    18361 atcaatagac aaagctctag ttttaagcac aacttgagat accagtgggg taataaaaca
    18421 ggtactattg caagcgatgt ggatacttcc gccgtttgga ctatccctct tgattttgct
    18481 agtgacatcc caaactcaac aagtggtagc ggcactatct atgtagatac ctacaatgac
    18541 tcaacgctaa ctggtactca acaagtgcca tttaccgtaa ctgtaccaga cagtatgaag
    18601 ccaacattat caagtattag cttgagtgat gctcataccg ttgcaggcaa cgtagtatca
    18661 agtgcggatt attttatcca agtatactct gacattagag tcaattttga atcggcaagt
    18721 ggctcttatg gttcgacaat caaaggctat tatgctgaga ttgtcgataa aggacagtct
    18781 actgaccaaa acggcggaac gctaggcaac atgctttatg atggtcaaat cactatcaga
    18841 gcaaaagtta ttgatagtcg aggtcgtgaa tcgcaaccca tggataaaac agtcacggtg
    18901 ctcaagtact ttccgccagc tttgtcgttt gatgttgcaa ggtctggtta tggttctgac
    18961 acgctaacag ttacaagacg agcgtctatc gctccgttaa gtgttttcgg aacacaaaaa
    19021 aataccatga cgcttagttt tagtgttgca gaattaggtt ctagttattt ttctgctaat
    19081 aacggctctg caagtggggc atgggcaaat gtttctagtt tggtaaactc gtctgccaat
    19141 ctttatggtg ctttctcgcc gacaaagtct tataccgtca aaggtatctt gtctgacaaa
    19201 tttagtcgca cagagtttac gtttgacgtc ggaacagaat cagttgtgat gtcaatcgct
    19261 aaaaatggca tcggattcca aaaaatttgg gaaaagggtg ctattgacgc taaaggagat
    19321 gcttatatta gcggtaagtt atttgtcaat aatacagagg ttaaaccgtc gtttgacaag
    19381 acagagattt taaacatggt ttatcctgtc ggagctattt acatgagcac atcatcagcc
    19441 aatccatcaa catttatcgg cggcacttgg caaagatatg cacaaggtag gactattgtc
    19501 ggtgtctccg aaaacgagac ggaatttaac tacgttggta aaactggtgg agccaagact
    19561 catacattaa ccaatgaaga gatgccaagt cacagtcacg gggataagac tattagctcg
    19621 ggtggtagac ctatctcatc aaatgctggt tgggacaata caaatgttgg tttatataaa
    19681 tcaacagatt acaatcaaat aaacgctttt aacaagtctt ccggaggtga tcaaccacat
    19741 aacaacttac aaccatacat cacaacgtac atttggctga gaacagctta gaggagaatt
    19801 tatgttagtt atcacatcaa attacccaga acaaatgcct gatggttccg tatctggtgc
    19861 gaaggtgatg cttgacggga ttgatgatca cgctggttgg catatcccgt tgacattact
    19921 aaaagaatat ttagacaaac ctcaaagcga ggttatgaca ctttgcgaaa agatgattta
    19981 tcaacaatta aaaccacaaa aagcattaaa cgaaaagttt gctaaactcg atgcaaccat
    20041 tgaaaagggt gaaaatatgc ttaaatttgc gactggaatt ttaaatcaaa ttatcgcagc
    20101 aaaagaagag gaaacaacag atgatgaaac tgttgaaaaa atggattaaa aggagatata
    20161 tcatgatgat taattatttc gcaatgcaaa ttgagtttgg ttggattact ttggaagatg
    20221 tacctaaaaa atatcgtgac aaagtaaaac agcttgttga atcagggaat atcggaacag
    20281 aataacgaga ggccttatat gacgatagat gttacactat ttgcgcaatg gtgtgtagcg
    20341 cttactgcaa taatagtatt tttaaaatgg gtgtctgcac ctgtcaaaca cgttttagac
    20401 aataataaaa aggctatgga agccttggaa agtgctatcg tcaaaatatc tgacgactta
    20461 aaagacaaca attttaaatg gacagaatcc aaaaaccatc gtgacagact acaaaaagtc
    20521 caagatcaac acgaaatcag aatcggaatc gtagaagata gacttattag tcatgacgag
    20581 cagctaaaaa cgctctggaa agtgaaagag gaaaagaaat aatggaagaa ttaatacaag
    20641 ttatcactgg ctcagcacta tcaattttga caatctttgc aggtattgta gtcaagttag
    20701 tcaaagatta tttgcttaaa aagggcggag aaaaagcggt caaaatcgcc gaaattgtgg
    20761 ctcgcaatgc cgtggaagca gttgagcaga ttgcttatga caaagacatc aaaggcattg
    20821 aaaaactgac agaagctaaa attgcggttc gtgacgaact gtctaaacac aatgtctatt
    20881 tgtcagacaa acaaatggaa gtctttattg aagcggctgt taaacgcatg aacgacagtt
    20941 ggaaaggtca ataatatggc aacagttagc gaagtaatta gctattggcg tggattagct
    21001 gatacaggtc aaggttatga tgccgacctt gcgtggggct ggcaatgtgc tgatgtgact
    21061 aatgggacaa ctactaactt ttttggagtg acgctttggg gaaatgcgat tgacttacta
    21121 gatagcgcga aggcgcaagg gttagaggtt atctatgacg cccctggaat aaatcccaaa
    21181 gctggtgatt tgtttgtgat gtttacttac ggacatcctt atgggcatac aggaattatc
    21241 attgctgata gcgacgggta cactatccag acgattgagc aaaatgttga tggctactca
    21301 gacaataacg gtgacggcat taacgatcag tttcaagttg gcggaccagc tcgctatgtc
    21361 actcgtgcct tttcagacgg cgacgggtat atcgtgggtt ggattagacc gccttatagc
    21421 gatacctcat ctgaaaagca atcacaatca caagcgccat caggattccg gaaattaaaa
    21481 gacgaggtcg gaacatttga ggttatggtc ccagcgctaa acgttcgccg tgaaccaagc
    21541 ttaaatggcg agattgtagc ttgttaccaa tacggcatga caggaactta tgattctgtc
    21601 tatgttggcg atgggtatat ttgggtgtct tatgtcggtg catctggcat gagaaattat
    21661 atggctgttg gagacgctga tggcgattac aatgtcaatc catattgtaa attttattaa
    21721 aagggggtta tatgggatat aactcaacca accttaaaca ggttgacggt ggcgatgtta
    21781 tcaaacaagg tgatacatcg tcacttttta gttttaattt gttggacgaa aacaacaacg
    21841 ttattgattt aaatggcaaa caagcgacta tttactttac acgtaatcgc aaaacctatc
    21901 taaccaaaac aactgatgtt attgacaaca aagttgattt tacgattaat aagattttgg
    21961 agattgggac atactatatc gaagtgcact gcgatggcta tgtctttccg tctgacgaca
    22021 gcgtaacgct tgatgttaga cgttctgggc aaaaatatgt cgttagcact gatttaatca
    22081 cagacacaac tatccaaaaa ctatcagctg atattgagta tttaaaatca aaagtgactc
    22141 aaaatcagca tctatttgag caagtgtcac cacaaacaga atggaccatt acacataatt
    22201 tgattaagta tccatctgtg acaattgttg atagtgcagg taatgaggtt tttggaagtg
    22261 tcgagtacat tagcacaaca aaaatcatcg ttagattctc agcgccattt gctggcaaag
    22321 ccattttaaa ttaaaggaga ttacatgagt acatttttaa caaatatcaa ccttaataaa
    22381 aacgagttgc aaaatgcagt cgtacaaaat ttagctaaag aaccgcatga accaaaatca
    22441 ggacaatttt attttgacag tgctgctaat aatctaaaaa tctataatgg gacatcttgg
    22501 gttgttttag acaaggaaac agcagaaagc attgttgacc ttattaattc tggtaaatcc
    22561 aaaatcgata aatcaaaagt cgatggactt gaagaagcaa ttaatcaagc gtctatgact
    22621 ggtcaagcta tcgcgaccgc tttaaacgaa gatactggtg gcgttactgt aaaccaaaat
    22681 aaaattacag ggcttacaga taaactagct gaaattgcga ctaacaaagc gtcagcgact
    22741 cgggagtcac aagatgcttt gcaagaagcc aaggtgtatg ctgaccaaaa agtagccgga
    22801 cttgttgata gtgcatcaga agattacaac acgtttaaag agctagaaac ttttattaaa
    22861 caaaacgcga aaggtctcgc agcaatctca aatgtagcca aaaaatttag taaaacgttt
    22921 ggcgatgcac gagctaaaga atttacaatc acacacaacc taaaaactcg tgatgttaac
    22981 gtcactatcc gagagactaa cttcccttat gcaatcgtct acgcagatat taaagtcaca
    23041 gacgaaaata gcatcttaat cgcaacaggt gcagaagtat taacgcaaaa tcagtatact
    23101 gtgacaatta ctggatagga ggccgctatg agatatattg gcgaatcaca actctcaaaa
    23161 gaccttgtaa caaaagatta tgtagataac aaagtgacta atctcgcgag taaggatgat
    23221 attgttaatt taggtaaaat taaggactcg caaacaggtg cgtttttgga agtcaaaata
    23281 gtcccaaacg gtcaagtccc ttatgacacc actggcatga ttgtgtttga acgctctgga
    23341 ggtaagtaat gtcagtacaa gagataccag ataaaatttt ttatcgatta atagagcaaa
    23401 cagtagaggc aaagctggtt tgggataagg acacacaaat aggtagctat tatatatcca
    23461 tacacgataa gtatagtttt gaatctggta gagatgttaa catctataaa atcttatcta
    23521 gcacacaggc tgatgtgtat aaagctaata agtataatta tcaatcatca tcaaaaccag
    23581 atactagcgt accacagccg aagccaccag agcaacctcc gctcaaaaac aagtctttgt
    23641 atcaatcaat cacgattgat ggactgccaa tagctcgcgt tatttgtaat aatcaggtca
    23701 tttggtctca atcagcagag cctcaggaag tttacaacat tacagcgtac gtagatgcta
    23761 gcgtatctaa gctaaatgac tctgccatta ttgattacaa tcgagatttt gcggcttacg
    23821 gcgtaaattg gagtcgagtt gatagagtcg ccgttgaagt cgacgataaa atagttgcta
    23881 agctacaagc tcctgtctat gattttggtg ttagaggtgg cacgacgatg tctgtcgaaa
    23941 aatgggatta cccatcaaat acaatagaca aatacaaaaa tccaacattt aaaattttgc
    24001 agcattagga ggtaaagctc ctttagataa gacaaaaccg ctcatcaaat gagcggtttt
    24061 tacattaata aaagaattgc aataaaattt ctaaatccag cagtaggtaa ggttgccaaa
    24121 agcaaaaagt tacttggttg ttaggtagac ttcaatttgg cttgtgtttg agtcaagcgt
    24181 ttcattatct ttatatatca taagatattt agattgagta aattctggtt cagggaaaaa
    24241 atcaaaccaa aaactttctt tattcttagg tatgaacttt atatatccag tttcatattt
    24301 agaaggtcca ttagtatata gttgcttatt atctgtaaga tattttctaa ctttatagtc
    24361 taattcttga gcagttacca tttttttatt tgtttcaata tcaaatgata ggctttggat
    24421 accatcgatt gatactttaa cgactatctt tttaggaatt tctaaatgat tcccttcatg
    24481 atttgttacc cctccgtaga tacatgcact cctttctgca ttttcacata aataacagag
    24541 atggtaatat tctacactat aaatatcaac gtttttatcc ttaaataaag ttgccatctc
    24601 ttggttctta agttcagttt ttaatttatc ataatttggc cctgaaacat tatatattaa
    24661 atcgtgagat aaaagttgat caacagattt cacattctcg tgagtaacag ggtcaccctc
    24721 ataaagaaaa tatatatttt gaaggttttt aactaaacta gatctgtgaa gttggcttgg
    24781 atcggggtct tgttgagcaa atacctcttg cgagattgtt agtccaagaa atgtcactaa
    24841 aacaaaaaa
//
LOCUS       DAUDYX010000028        24411 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.028, whole genome shotgun sequence.
ACCESSION   DAUDYX010000028 DAUDYX010000000
VERSION     DAUDYX010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24411
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.028"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            6..233
                     /locus_tag="VHD02_001102"
     CDS             6..233
                     /locus_tag="VHD02_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEQ5497718.1"
                     /translation="MTKKQRLKQEHLKPKKRLREERLKREYTEMYMADLIGLKNRKGY
                     SEKEDGLQPFKDYEMAIISQKFNVSESELFF"
     gene            284..1009
                     /locus_tag="VHD02_001103"
     CDS             284..1009
                     /locus_tag="VHD02_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HEQ5497719.1"
                     /translation="MNHLINVTLNENQEPVVSARDLHKELKVKTRFSEWVKQNFKILE
                     EGYDFTSVVGTTVVNNGAVREIQDYVLSLDAAKNLAMVSKTDKGKEVRKYFIQVEKDF
                     NSPEKIMARALLMADKKVHKLESQIEADRPKVLFADAVSASHTSILVGELAKLLKQNG
                     VNIGATRLFTWLRKHGYLIKRNGRDWNMPTQKSVELGLIRVKETSITHSDGHITVSKT
                     PLVTGKGQQYFINKFLNQEYLPV"
     gene            1041..1307
                     /locus_tag="VHD02_001104"
     CDS             1041..1307
                     /locus_tag="VHD02_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497720.1"
                     /translation="MEEKKFFTTYEMDLMNSVVTLQKSILTQTEHLSDQLTKKLHHLE
                     DYNSPIDDEAIRLAEVTAEFYKLLIKSPSVGATVKEIVSEGHKR"
     gene            complement(1242..2048)
                     /locus_tag="VHD02_001105"
     CDS             complement(1242..2048)
                     /locus_tag="VHD02_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HEQ5497721.1"
                     /translation="MLNIDTQFVDTIGKILSDYVSHSEITRMGEVLGYPQNDQNSGFN
                     KHKRVHNLVSDILNRTQNTDNIKLVIEYVCNPLRYINEVSIFEQLRTAINIPLSLKGL
                     IVSDSGQIVTTTTSKTLSEAKKRFETLDSRLKELKVHPHVLKFCTQELLQENYFHAVF
                     EASKGVFHRIRLLTGSAMDSASLIDQCFKPGEPIVIINGNKLQTLDEQSEYKGLKNLL
                     LAIAHLYRNSKAHKLKYYNPDNLNDALTALTLMSLAHNLLDSCSNTRRLD"
     gene            complement(2190..2426)
                     /locus_tag="VHD02_001106"
     CDS             complement(2190..2426)
                     /locus_tag="VHD02_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497722.1"
                     /translation="MIFFFLSVVSKHINQNFNVDNQLVEFVVAELNQMNAPITQKQAQ
                     HIVNILEYVSKSTSKSTIAAMTNALLELGVLKGD"
     gene            2569..2880
                     /locus_tag="VHD02_001107"
     CDS             2569..2880
                     /locus_tag="VHD02_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEQ5497723.1"
                     /translation="MPKAEITYRAVDVNETASHGDYKHLIQQWEGLTVATAKQWATEM
                     RDHPDFKQFVLNPTHRIVFIDYEGFKLFVQWKSRNRYKTKKETLSEMLENIKFEKRVG
                     A"
     gene            2882..3067
                     /locus_tag="VHD02_001108"
     CDS             2882..3067
                     /locus_tag="VHD02_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497724.1"
                     /translation="MNKLELFLLVTTIILAIITRVQHEVIKKHNSPENKRRIFREVAL
                     ENSKGWSEKRSGGKVVS"
     gene            3550..3936
                     /locus_tag="VHD02_001109"
     CDS             3550..3936
                     /locus_tag="VHD02_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEQ5497725.1"
                     /translation="MDEKKLFENFQLTFGRMISPFEIEDIQKWIHEDNMPIEVVNLAL
                     REAVENNKISWKYINKILVDWYKSGDTTVEKVRDRLQRFDDSKKQRSVTTSNVPSWSN
                     PDYKEPDLEEFALGSMDGIEDGSGDF"
     gene            3917..4150
                     /locus_tag="VHD02_001110"
     CDS             3917..4150
                     /locus_tag="VHD02_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497726.1"
                     /translation="MDQEIFNFFNKQIKKDFGKTASKETFAKFASYCAEGIEKNGVKP
                     IFNWINLYAFGTDITTAEADRLRIERYKQENVL"
     gene            4147..4287
                     /locus_tag="VHD02_001111"
     CDS             4147..4287
                     /locus_tag="VHD02_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497727.1"
                     /translation="MTKQHRETLIWYRASHQERERLLDFGLVDKARYVTLLRQLRKKY
                     AI"
     gene            4296..4502
                     /locus_tag="VHD02_001112"
     CDS             4296..4502
                     /locus_tag="VHD02_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497728.1"
                     /translation="MTPEQAEKAKIRAKQELETFSIYLDQAVDELGGVLTSREVFLAA
                     GFTYLGAGQTDIHAAVEGLCEQIQ"
     gene            4558..4887
                     /locus_tag="VHD02_001113"
     CDS             4558..4887
                     /locus_tag="VHD02_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497729.1"
                     /translation="MNIKEKIVVLRNTEDGSFLKSFKNKKDVLAYNVELTDSIQLASF
                     LPEEAYNIQKEKIDNLAETLGCDVVVIEASYDLKFIDGEDVPELTKEQKVKSMVNGMF
                     EQVFGGE"
     gene            4890..5681
                     /gene="bet"
                     /locus_tag="VHD02_001114"
     CDS             4890..5681
                     /gene="bet"
                     /locus_tag="VHD02_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HEQ5497730.1"
                     /translation="MANQLSTQQVKRDITTDPTLLTGADIKKYFDPQNLLSEKQVGQA
                     LALCKGRNLNPFANEVYIVAYKNNSGTDFSLIVSKEAFMKRAERCEGYDGFEAGITVM
                     RNGEMVEIEGSLKLPDDVLIGGWAIVYRKDRSHRYKVTVDFNEYVKLDKYGNPRSTWK
                     SMPGTMIRKTALVQTLREAFPDELGNMYTDIDGGDTFDAIKDVTPQETQEEVRARKMA
                     QIEQYKQEQTQKQTQKADTSYPVDEVSEHTDDPVQGELLDGELEY"
     gene            5691..6719
                     /locus_tag="VHD02_001115"
     CDS             5691..6719
                     /locus_tag="VHD02_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HEQ5497731.1"
                     /translation="MQELQLKVTQAQVEIIDREKFEQNINEVVAKYQNYAVTAGTIKD
                     DKQVLADLRKLKKQLSDERIKVKKELSKPADDIDGYIKQASKPLDDTIDKIATDVKEF
                     EDHQKALRLDTVKSYLSNKASEYMLDPRIFDEKAMEYTKAGNFMADGVTLKKVTMKSL
                     EDLVTFEYQKEQEVEKAKATVSGQCAEYGMTDQPYIRMLKEMTLVEVLGQIKADYLAE
                     KQKQEMRKAEEEREQLLAAQQTKEQEQAQKSAEIPQIDKETGEILDGWQLSQNNQETL
                     TGAENEFKKYNQKMTLEVYFEDTAEKDRFKTGLTQLGFDFKKNYQVSGYQNIEPLTQA
                     ELARRCGW"
     gene            6915..7253
                     /locus_tag="VHD02_001116"
     CDS             6915..7253
                     /locus_tag="VHD02_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497732.1"
                     /translation="MEQIKITETGMVVISDKALKTFVIAGHLSERWEFTSKFKKLDEP
                     SLDENGDLFEPVYELMLEARSKGQISITSSYCGKNHKKDTDEIIKVFSFIEDNKRNIF
                     ENLGIRGVLE"
     gene            7250..7762
                     /locus_tag="VHD02_001117"
     CDS             7250..7762
                     /locus_tag="VHD02_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497733.1"
                     /translation="MSNLVLSLDISTSGTGWALFKGSDLIQSGVLKHKSKSYFERGRY
                     MASQLGLIQSRALKKYDCYFSTIAVEKNSVMEPNQQSMLKIGIVTGIILGRLIADNVA
                     FINVSTWRKHWKFSYKDRSKKAMKAQSKEKALEYCGKTVKDDEADAILIGSYYVNQGY
                     LDELETHDYY"
     gene            7749..7943
                     /locus_tag="VHD02_001118"
     CDS             7749..7943
                     /locus_tag="VHD02_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497734.1"
                     /translation="MTTTKKHVVRVYNKGITATYAIYDKKLFKEHEFATKNEAMRFIR
                     QLELANDKRATEYYLREVEK"
     gene            7940..8185
                     /locus_tag="VHD02_001119"
     CDS             7940..8185
                     /locus_tag="VHD02_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497735.1"
                     /translation="MIPNFRGFNKKTKKMYSIDGFKSSERKIYRCSLADDEFRSGRLE
                     TFHFVEDNLVEKCNRPPERFYRQKQVRKSDRRLMARQ"
     gene            8237..8743
                     /locus_tag="VHD02_001120"
     CDS             8237..8743
                     /locus_tag="VHD02_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEQ5497736.1"
                     /translation="MNIEEAKELVDNSKFYGKTSSVIKAEVRDIIDQLNQPKPEVPQC
                     VADWIEECKEEDLTLSLAYDVDAFGEVAKWLYDTNDSTNIDLFAQAWLAYPNITIEKE
                     KLYTVEIPDPNHDVGTVILSKNKHREAYIAIDYTGSWKERKANHLTESEIRKDFDWAW
                     QFAKEVTE"
     gene            8740..8910
                     /locus_tag="VHD02_001121"
     CDS             8740..8910
                     /locus_tag="VHD02_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497737.1"
                     /translation="MTEELGVLYSEKWHKYYLYKTCRYISYVDNPHQATKCTRKQAEQ
                     FPQFKWVPLTKL"
     gene            9184..9624
                     /locus_tag="VHD02_001122"
     CDS             9184..9624
                     /locus_tag="VHD02_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEQ5497738.1"
                     /translation="MTFFPEIDIQKTKSNAKRKLREYPRWRRIANDVDTQKVTATYSF
                     EPRQPHGVPSKPVERLAINRVSAEQELDAIERAVNGIFDPEYRLILIDKYLLTYPKTD
                     CDIYTNLGYEKSQYYNMLDNALMSFSELYKEGVLLIEKLEKSWN"
     gene            complement(10319..10702)
                     /locus_tag="VHD02_001123"
     CDS             complement(10319..10702)
                     /locus_tag="VHD02_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEQ5497739.1"
                     /translation="MLLYPAVFTHDNETNSIGVRFPDIPEAITFGNDLGHAYEMAVEV
                     LGFALEDYTDYPKASSVSDLKGQYRDSDIALIGIDMIAYMKKYHSKKVRKNVTIPEWL
                     NDAAEDKNLNFSQVLTEALELKLQA"
     gene            complement(10747..10935)
                     /locus_tag="VHD02_001124"
     CDS             complement(10747..10935)
                     /locus_tag="VHD02_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEQ5497740.1"
                     /translation="MPRTAREIIKLLKKNGFVKVSQNGSHAKYKNFETGNITIVPMHK
                     GDIPKGTEDDILKKAGLK"
     gene            11036..11377
                     /locus_tag="VHD02_001125"
     CDS             11036..11377
                     /locus_tag="VHD02_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEQ5497741.1"
                     /translation="MRPQKLIIAGGRRMTVDYDDRSAEYRDYNRNRWKYDKQVKQFYN
                     SKIWRETSKQVLLQNDYVCAMCGGEATMTDHIVSIKKDWNKRLDRNNLQASCKACNDS
                     KAIRERRKNNY"
     gene            11545..12012
                     /locus_tag="VHD02_001126"
     CDS             11545..12012
                     /locus_tag="VHD02_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEQ5497742.1"
                     /translation="MGRNLKLVETTKKHLTKEEKIVRETAQNKASDGLKKLQKTPPEH
                     FNNVAKYEYRRIIEDLQNLPLRNLDRGLLELYCTWYAIYKETSKKLDEVGYFTNDPDK
                     GLIPSPLILTLEKAAANIRSSASQLGLTVDSRMKMFIPKEEEKPKSIFDKFGG"
     gene            12027..13781
                     /locus_tag="VHD02_001127"
     CDS             12027..13781
                     /locus_tag="VHD02_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEQ5497743.1"
                     /translation="MTIEYDYSAISDIYKDDAFYYAKMVVDEQIKSSKKVFKACLRHL
                     NDLKKIDGDNFKFIYLPEKAADPINFIEILPDVKTGKPYPLAMFQKFIIGNLYGWRKK
                     TDHSLRRFRKAMISVARKNGKTILIAGILLYEFLFGHNPSMSRQLFCTANDRTQAKIA
                     WDMAKKQLSSLRAKDADVRKATKIVRDELKNLHDESYIRALSRDTGAVDGFEPYVGVL
                     DEFAASKTNEMLELLESGQGQLDNPFILIISTAGMDLNVPMHTIEYPYITKILDGEIV
                     DDGYFGYVAEQDNEEEIKDESNWIKSNPILEVKTLHDKIMDYLRTRRRVSLETGEINK
                     VLIKNFNMWRQSSEESYIDKQSWELAQIDKPDTTKRRVWLGVDVGRVSDLFAITPVIM
                     MDDYWYLDSFSFVATKYGLTAKEKRDGVSYSNLERQGYCEITTLESGVIDDERVLEKI
                     EEMVYSNDWEVNGICFDPYQFGTLLTMIEKRHPEWPLIEVNQTTMVLNMPTKQFRDDL
                     KKGKIKHSGNPLLTMAVNNAYIKTDNNGMRIDKNKNSNKIDPLDAALDGYAVCYLEPF
                     DGSGYWTSEKILGGETLF"
     gene            13778..13945
                     /locus_tag="VHD02_001128"
     CDS             13778..13945
                     /locus_tag="VHD02_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497744.1"
                     /translation="MIGFILKNIHTLILLVGLGLLIHGLFLFGDKVGFIASGLILIIL
                     AIYVDSIGGRK"
     gene            13942..14208
                     /locus_tag="VHD02_001129"
     CDS             13942..14208
                     /locus_tag="VHD02_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497745.1"
                     /translation="MNKRIKKKRKIEQAIVLLIAENAMQAEAIKSQDRQIAVLTGIVQ
                     HNAMATNEELATVKAATLDNQVAIKAIGDDVDYIKKNYKRKWRT"
     gene            14242..15462
                     /locus_tag="VHD02_001130"
     CDS             14242..15462
                     /locus_tag="VHD02_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEQ5497746.1"
                     /translation="MSFFQPLGSSKVSYDDYISSVLAGDVSQQYLGVSALKNSDVLTA
                     TSIIAGDIARFPLLKKDITGDIIHDEDINYLLNVKSTNNASARTWKFAMAVNAILTGN
                     SFSRILRDPKTGQALQFQFYRPSETTVEETDNHEIIYTFTDTLTAKQVKCFAHDVIHW
                     KFFSHDTILGRSPLLSLGDEIDLQKGGINTLIKFFKDGFSSGILTMKGAQLSGEARQR
                     ARQDFEKMREGSVGGSPLVFDSTMEYTPLEIDTNVLQLITSNNFSTAQIAKALRVPSY
                     KLGVNSPNQSVAQLMEDYVTNDLPFYFDAITSELGLKTLNDKDRRLYHIEFDTRSVTG
                     RNVDEIVKLVNNQILTPNQGLVELGKQKSTDPNMDRYQSSLNYVFLDKKEEYQDKVGI
                     KGKGGEVNAKEDKS"
     gene            15440..16105
                     /locus_tag="VHD02_001131"
     CDS             15440..16105
                     /locus_tag="VHD02_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEQ5497747.1"
                     /translation="MPKRINLKGPLISNNSQEVYDYYGMEAVSAKSIFDNLPEDNSDI
                     ILEVNSNGGLVTVGSEIYTALRNYKGKVTAEITGMAASAASVAVMGADKVVMSPTAQM
                     MVHKALFNWVSGNSDDLDKASNALKSSDKAIVNAYVAKTGLSEDEIMDLMRNETFMSA
                     QDAVEKGFADEVMSFEAVASIDNSMLPQAVIDDYYASRNKRKKEISNMLLEIEKEEIL
                     QGL"
     gene            16130..17311
                     /locus_tag="VHD02_001132"
     CDS             16130..17311
                     /locus_tag="VHD02_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEQ5497748.1"
                     /translation="MFEEKIKEIKANIASLNQTIATKTAEVKNALESDDLETARSIKA
                     EVEETKASLAEAENDLKLYESSIEKGGAENTGGKEVPQETKTYRESVNEFIRSKGKAV
                     NEDLRFEGKNEVLISMNEVTPVAPKTDGVKKADVKPVSSEEILYTPAREVKTVVDLKQ
                     FTSIHPAKKASGKWPVLQRATDKMVSVEELEKNPKLAKPQFKEVKWEVKTYRGAIPLS
                     QESIDDADVDLVGIVAETIGQMKVNTTNGAIAEVLKKFESKEVKDLDEIKKLLNVDLD
                     PAYNVSFIVSQSFYQTMDTLKDKNGRYLLQDSITSVSGKVFLGKPVFVLADEVLGENK
                     AFVGDFKRGVLFADRKDLGLRWADNEIYGQYLQAVLRFGVSKVDEKAGYYVTFTPSES
                     L"
     gene            17262..17444
                     /locus_tag="VHD02_001133"
     CDS             17262..17444
                     /locus_tag="VHD02_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497749.1"
                     /translation="MKKLDTTLRLHPVKASEKEAKPTSKSTVEEIKRYLTSQGIDFSG
                     KTLKSDLLALAGVEEV"
     gene            17447..17749
                     /locus_tag="VHD02_001134"
     CDS             17447..17749
                     /locus_tag="VHD02_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEQ5497750.1"
                     /translation="MAVSKELLDSVKLYCKIDFDFEDDIIKEMIESAQEQICFAIDDG
                     STADTFKDSAKFALAVKKQVKEEYDHRGLSADSFRYPLANGVLNIIHQLRLRGDDS"
     gene            17746..18093
                     /locus_tag="VHD02_001135"
     CDS             17746..18093
                     /locus_tag="VHD02_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEQ5497751.1"
                     /translation="MITRKMNTRITIFSQSGGQNDDGEVVSAVRKDVYTCWAEVLKTQ
                     LRDFNYKSKFQNASDLPTNKDTKVFLIRYNPKLSIDNTMFVEFNNRIYKIDKIESDES
                     SKDITMISGVSMS"
     gene            18090..18467
                     /locus_tag="VHD02_001136"
     CDS             18090..18467
                     /locus_tag="VHD02_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEQ5497752.1"
                     /translation="MTKGLDEILANLTKLEVKAPKTAKSAVTEVAKEFEKALKANTPV
                     YEIETEERLQEDTVISGFKGANVGIVSKEIGYGKATGWRAHYPNDGTIYQRGQDFKEK
                     TINQMTPKAKQIYAEKVKEGLGL"
     gene            18464..18889
                     /locus_tag="VHD02_001137"
     CDS             18464..18889
                     /locus_tag="VHD02_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497753.1"
                     /translation="MIAETTAYKLLSNDKTLNELLDKLRGGSFKNGFKQGIFTYDIPD
                     NPIDLRKAELAPFMRINTTLDGPADYADDEILCNEQRITINFWCKTASEADQISKCID
                     DILKKGGFERYTANEKPRYKDSDIDLLMNVRKYRYFDFY"
     gene            18906..19517
                     /locus_tag="VHD02_001138"
     CDS             18906..19517
                     /locus_tag="VHD02_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ5497754.1"
                     /translation="MGKVKFGLRDFYYGVLDDKDEVTDKKKGIKHLPGMKSAKLDITN
                     ELVTVAADDGPYVVLSGGISETKLEIELLDLTSDARKDFFGITVEKGVEKYNKSLTPK
                     DVTCVFRTSDENGKAIWVGLLKGKFNLPGMEAQTKEGTPDPKPDSVTGNFVARGKDGD
                     VILIGREDASDFKINDFLKCVFNGCQDADIKEIPIAEQALLPM"
     gene            19570..19896
                     /locus_tag="VHD02_001139"
     CDS             19570..19896
                     /locus_tag="VHD02_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497755.1"
                     /translation="MYEITLKKGGVDKEFKKDFINVEDNLLAIEHQVRQSALFGDDKR
                     RLESKAHRKLNESYLQMFVDMYAGQFTVDDLKQSDMSVLNTLNDLYIAALGGEQEEEQ
                     AEKKEQ"
     gene            19926..20093
                     /locus_tag="VHD02_001140"
     CDS             19926..20093
                     /locus_tag="VHD02_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497756.1"
                     /translation="MWIQSLLKNGYTILDIKKMRLSDIELMVQALEIESVEKEEVIET
                     TLDKAFPFLFG"
     gene            20106..24029
                     /locus_tag="VHD02_001141"
     CDS             20106..24029
                     /locus_tag="VHD02_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEQ5497757.1"
                     /translation="MGNIGDLVATATLDISPFMSNTRNLKTYMKGLDNSLKAVENSFR
                     GHGGRIKGLKAVYTETGSALKGYQDLLKTQSQKYSDLKREIGDVNNATAEQKQKLIGA
                     KSAMLDTAAKVTELQNRLRALATETSVFTRFGKAAERVGGKMKSFGDSVAGVGAAFTR
                     GVTAPIVAGAGYAIKAAVDYESAFAGVKKTVDETATVSYAKLSQGIRQMAKELPASAV
                     EIAHVAEAAGQLGVKTGDILSFSRTMIDLGESTNLSAEEAATSIAKIANITGLASSEY
                     SRFGSAVVALGNNFATTEKDIVAMTNRIAASGKLAGLTNQEMLALATAMSSVGIEAEA
                     GGTAMTQSLSAIERAVSAGGEDLTKFAQIANMSSADFAKAWKEKPIVALQEFIKGLGQ
                     LDKKGESATKVLDELGLSGIRQSNMLKSLGLASETLGKALGISNKAWKENTALTDEAN
                     KRYETTESKLKMLKNEINDVAIEFGGPLVDALRNGLEAGKPIIQMAADLAKQFNSLDK
                     EQQQQIVKWGLIAAAAGPALSIFDKGVGIIGSTIQGIGKMSQGLEALSGWLRTFKSGA
                     VAASVGAEAAATSMGGMAGAVALLSNPVTWGVLLGGAAIVGIGLIADSMYKAQKRTEE
                     WGTAVSQTEAVALSNFKKKVDETNTSLQMFEAGASSVKKVTEAFDDLVGSIEKLAQSK
                     LDKNINLAKKLGLSEETINVLKSKTEAVVNNVKGMNAQIKAIMEKHNGDMSKLSETEK
                     ELVLRNQREMISTQLDLMKFSASEKKALTAALNNELDALNARQLEKVSENTVKMLDKE
                     NSAYKTKKAELKEILKQFGSDTSKLSAEELAARQEVLNRLTELNMQHNLKTKALSDQY
                     LAIQRERVQRLKESGKSQEEIHRGISQMASDMAQKLGISYDDAYRKLAYYTEKSGETL
                     KVLSRNTANATAEVAAANAQWDSLFTSDNPQQSLNELLSTAEGWNSFEIMVKNADVEP
                     TGRAALAEMLVAGGQWENMTLEQKKLVVDGQQAMIEIFDSKELLAQWQALTPEEKVLL
                     AKNLTQEPTMSAQQALDSVKQTVPADVNASDKTAGETQSAQNKIDNVKQKAPADVKAS
                     DKTGPDVASANRAVNSPKQNSPAVIRAQDNASGVAENVIWSLARIPRSVTTTITTFVR
                     KIFGHEKGTDFHPGGLAMVNDQKGPLYRELVTLPTGESFIPSGRNVILPLPRGSKVLK
                     ASRTKQLFPHYANGIGFDDTKIANLATRLKNVHDKGQVIVNNDPQIAELIKLLKDRDD
                     RNVTNNYTLNATNSSSSGDMFSQENMRRLIRELAYYTSGEGGRLA"
     gene            24029..>24411
                     /locus_tag="VHD02_001142"
     CDS             24029..>24411
                     /locus_tag="VHD02_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ5497758.1"
                     /translation="MRYIEFNGIKSNDLGLLLERERSVKSTSNDVNLIEVDGRDGVLI
                     KDNERLKAVEQDFPFSLVGDVVVNQQKISEWLHVKGWHDLALSWDKDYIYRASVVNLF
                     EIDEILKQFGRLKINFLIHPIKYLKT"
BASE COUNT         8518 a         4028 c         5350 g         6515 t
ORIGIN      
        1 ggggaatgac gaaaaaacaa cgtttaaaac aggaacatct caaacctaaa aagcgtttaa
       61 gagaagaacg tttaaaacgt gagtacacag aaatgtatat ggctgattta attggtttaa
      121 aaaatagaaa aggttactct gagaaagaag atggtttaca gccttttaaa gattatgaaa
      181 tggctatcat ttctcaaaaa tttaatgttt cagagagtga attatttttt taaacaaaaa
      241 tatctcaaaa cgagataaag cgtacaacac tagaaaggac aatatgaatc atctaattaa
      301 cgtaacacta aacgaaaatc aagaaccagt agtaagtgca agagacttac ataaagaatt
      361 aaaagtcaaa actcgtttta gtgagtgggt aaaacaaaac tttaaaattc ttgaagaagg
      421 atatgatttt acaagtgtag ttggaactac ggttgtaaat aatggggctg tcagagaaat
      481 ccaagactac gttctctcac ttgatgctgc caaaaatttg gcaatggtat ccaagactga
      541 caaaggcaaa gaagtccgta aatacttcat ccaggttgaa aaagacttca acagtcctga
      601 gaaaatcatg gcaagggcgt tgctgatggc ggacaagaaa gtgcataaat tagagtctca
      661 gattgaggcg gaccgtccca aagtactatt tgctgatgcc gtaagtgcta gccatacctc
      721 tatcttagtc ggagaacttg caaagttact caagcagaat ggggtaaata ttggagcaac
      781 ccgtctcttc acttggcttc gtaaacacgg ctatcttatc aagcgcaatg gccgcgattg
      841 gaatatgcct actcagaaaa gcgtagagct tggacttatc agagtcaagg aaactagtat
      901 cacccattct gatggccata ttacagtaag caagacacct ctggtaaccg gaaaaggtca
      961 gcaatacttt atcaacaaat ttcttaatca ggaatatcta ccagtttaga aacgtaacaa
     1021 aactaacgaa gggagaaaat atggaagaga aaaaattttt tactacatat gaaatggatc
     1081 tgatgaattc agttgtaact cttcaaaaat caatcttaac gcaaacggaa catttgtcag
     1141 atcaactaac aaaaaagctt catcacctag aagactacaa tagtccgatt gatgatgaag
     1201 caattagatt ggctgaggta acagcagaat tttacaagtt gttaatcaag tctcctagtg
     1261 ttggagcaac tgtcaaggag attgtgagcg agggacataa gcgttaaggc cgtaagagcg
     1321 tcattgagat tatctggatt gtagtatttg agtttgtgag ctttagagtt gcgataaagg
     1381 tgagcgattg caagtagcaa attttttaat cccttatact cactttgttc gtctagagtc
     1441 tgcaatttat taccgttgat aatcacaatg ggttctccgg gtttgaaaca ttgatctatc
     1501 agactagctg agtccattgc tgagcccgtt agcagacgaa tacgatgaaa aacaccttta
     1561 cttgcttcaa atacggcatg aaaatagttt tcttgtaaga gctcttgagt gcagaatttt
     1621 aaaacgtgag ggtgtacttt taattctttt aatctactat caagtgtttc gaagcgtttt
     1681 ttggcctctg ataaagtttt tgaagtagta gtagtaacaa tttgtccgct atctgataca
     1741 atcaaacctt ttagcgagag cggaatgtta atggcggttc ttaactgttc aaaaattgaa
     1801 acttcattaa tgtatcttaa aggattacag acgtattcaa tcacaagttt tatattatct
     1861 gtattctgtg tcctatttaa tatatcggac acgagattgt gcacccgttt gtgcttgtta
     1921 aatccagaat tttggtcgtt ttggggataa cctaaaactt cgcccattcg ggttatttca
     1981 gaatgcgaaa catagtcaga tagtatttta ccgattgtat caacgaattg ggtgtcaata
     2041 tttaacataa caaacctcgt ttttatttaa attataccac agaaaggaga accacatgag
     2101 accaaaacga tatccgtata gcggagagaa aaagagagcc tatcataaga tagaccctga
     2161 actgataaaa accggtaaag ttgattgcat caatctccct taagtacgcc aagctctaac
     2221 aatgcatttg tcatagctgc gatagtagat ttagaggttg acttagagac atactccaaa
     2281 atattaacga tatgctgagc ttgcttttgt gtaataggtg cattcatttg attaagttcc
     2341 gcaacgacaa attcaactaa ttggttgtca acattgaaat tttggttgat atgttttgag
     2401 acaacagaaa ggaagaaaaa aatcattgaa atttgatgat gtcgttaaaa tcgccgatgt
     2461 cttaggtgtc agcttggacg aattcagata acaaaaaaag tccgacggca atcggactca
     2521 aaactagatt aatttactta attataccac aagagagagg atattaatat gccaaaagca
     2581 gaaattactt atagagcagt tgatgttaat gaaacagctt ctcatggtga ttataaacac
     2641 ttgattcaac aatgggaggg gttgacagtt gctactgcaa agcagtgggc gacagaaatg
     2701 cgtgatcatc cagactttaa acagtttgta ttaaatccaa cgcatagaat cgtattcatt
     2761 gattacgaag gattcaaact gtttgtacag tggaaatctc gcaatcgtta taaaacaaaa
     2821 aaagagacac tgtcagaaat gcttgaaaat attaaattcg aaaaaagagt aggagcataa
     2881 catgaacaaa ttagaactat ttttattagt aacaacgatc atcttagcaa tcatcactag
     2941 agtacaacac gaagtcatta aaaaacataa ttcgccagag aataaacgca gaatttttag
     3001 agaagtggct ttagaaaaca gcaaagggtg gagcgagaag cgttctggag gaaaggtggt
     3061 cagctgatgc agtacatttt tcaaaaatac gacaagtagc tatacatccg ttagcaacga
     3121 atttatagat gattgtgaat tatccctaaa atcaaaaggc ctgcttatgg taattcttag
     3181 aaataaagaa gattggagag tatatccaga ggaattggct agaaggtcca gagatagcgt
     3241 gagagttgta aggacttgct tagaagaatt agagagagtc ggatatgtga gaacttacag
     3301 aaaatcttta gggcgaggga aaggcataca atattataga ttttgctcag ataaaaagat
     3361 atccgaacaa gcatttaaaa aaatgattag tgattttaca acttaacgtt ttacaaagtt
     3421 gtattttaca aagttgtatt ttacaaagtt gtaaaacgta acactaataa ttactaactt
     3481 ataaataaat actaaataac aataaatact aacagataat aagcatcatc atcaacatca
     3541 ggaggagcta tggacgaaaa aaagcttttt gaaaatttcc aattaacttt tggacggatg
     3601 atatcgccat ttgaaatcga agatattcaa aagtggattc acgaagataa catgccaatt
     3661 gaagttgtca accttgcctt aagagaagcg gtagaaaaca acaaaatcag ttggaagtat
     3721 atcaataaaa tcttagttga ttggtataaa tctggagata cgacagtaga aaaggtcaga
     3781 gacaggttgc aacggtttga cgatagtaaa aaacaacgaa gtgtaactac ctcaaacgtc
     3841 ccaagctggt cgaatccaga ctacaaagaa ccagatttag aagaatttgc tctaggaagc
     3901 atggacggta tagaagatgg atcaggagat ttttaatttt tttaacaaac aaatcaaaaa
     3961 agattttggt aaaacggcga gtaaagagac ttttgctaag tttgctagtt actgcgctga
     4021 aggaatcgaa aaaaatggag ttaagccaat ttttaattgg ataaacctat acgcttttgg
     4081 aactgatata acaacagcag aagcagaccg attaaggata gagcgatata aacaggagaa
     4141 tgtgttatga caaaacagca tagagaaacg cttatctggt accgagcaag tcatcaagag
     4201 cgtgagagat tgcttgattt tggactagtt gataaagcac ggtacgtgac actattgcgg
     4261 caattgcgta agaaatatgc gatttaggag gaaatatgac accagaacaa gcagaaaaag
     4321 caaaaatcag agctaaacaa gaacttgaaa cgtttagcat ataccttgac caggcagttg
     4381 atgaactcgg tggagtttta acttcgcgag aagtcttttt ggcagcggga ttcacctacc
     4441 ttggcgcagg tcagacagat atacatgctg cagtcgaggg attatgtgag caaatccaat
     4501 gattttaaat ttagtgaaga ctgggagagc aactaaaaca caaaaagagg aaatgaaatg
     4561 aacatcaaag aaaaaattgt agtgctaaga aacactgaag acggaagttt tttaaagagt
     4621 ttcaaaaaca aaaaagatgt acttgcttat aatgtggaac ttacagatag cattcaactg
     4681 gcatcatttt taccagaaga agcttacaac atacaaaaag aaaaaattga taatttggca
     4741 gaaacgcttg ggtgtgatgt tgtagttatc gaagcgtcat atgacctaaa atttattgat
     4801 ggcgaggatg ttccagagtt aacaaaagag caaaaagtta aaagtatggt aaacggaatg
     4861 tttgagcagg tttttggagg tgaatagaga tggcaaatca attatcaaca caacaagtta
     4921 aacgtgatat cacaactgat ccaactttgc tgacaggagc tgatattaaa aaatattttg
     4981 atcctcagaa cttgcttagc gaaaaacaag tagggcaagc tctggctttg tgtaaggggc
     5041 gcaaccttaa tccatttgca aacgaagttt atattgtggc ctataaaaat aatagcggca
     5101 cagatttcag tttgattgta tctaaagagg catttatgaa acgtgctgaa cgttgtgaag
     5161 gatatgatgg attcgaggct ggcattactg ttatgagaaa tggtgagatg gttgaaattg
     5221 agggctctct aaaactacct gatgatgttt tgataggtgg atgggccatt gtttaccgca
     5281 aagaccgctc acatcgatac aaagttactg ttgactttaa cgaatatgtc aaacttgaca
     5341 aatatggtaa tccacgaagt acttggaaat caatgccagg aacaatgatt agaaaaacag
     5401 cacttgttca gacacttaga gaggcttttc ctgatgaact cggtaatatg tacaccgata
     5461 ttgacggtgg cgatactttt gatgctatta aagatgtgac tcctcaggaa acacaggaag
     5521 aagtcagagc acggaagatg gcacagattg agcagtacaa gcaagaacag actcagaaac
     5581 aaactcaaaa agcagacact agctatcccg ttgatgaagt gtctgaacat actgatgatc
     5641 cagtacaagg agaactactt gatggagaac tggagtatta ggaggaaacc atgcaagaat
     5701 tacaattaaa agtcacacag gcacaagttg aaattattga tcgtgaaaaa tttgaacaaa
     5761 acatcaacga agttgtggca aaataccaaa attatgcagt cacagctgga actatcaaag
     5821 atgacaagca agtcctagct gatctacgca agctcaaaaa acagttgtct gatgagcgta
     5881 tcaaagtaaa aaaggaactc tcaaagcctg ctgacgacat tgatggatat atcaaacagg
     5941 caagcaagcc actagatgac acgattgaca agattgcaac tgatgtcaaa gaattcgaag
     6001 accatcaaaa agcgctgcga ttagatacag taaagagcta cttgtctaat aaggcatccg
     6061 agtatatgct tgacccccgt atttttgacg aaaaagctat ggagtacacc aaagctggca
     6121 attttatggc ggacggtgta accctcaaaa aagtcaccat gaaatctctt gaggacttgg
     6181 ttacctttga atatcaaaag gagcaagagg tcgaaaaagc aaaagctacc gtctcaggac
     6241 aatgtgctga gtatggtatg actgaccaac cttacatccg tatgctaaaa gagatgacgc
     6301 ttgtagaggt gctaggtcag attaaagctg attatctcgc tgaaaagcaa aagcaggaaa
     6361 tgcgaaaagc cgaggaggaa agagagcaac ttttagcagc tcagcaaact aaagaacaag
     6421 aacaggctca gaaatcagca gaaataccac aaatcgacaa ggaaacaggc gaaatcttgg
     6481 atggttggca attatcccaa aacaaccaag aaacgctcac aggagctgaa aacgagttta
     6541 aaaagtacaa tcaaaaaatg acacttgagg tgtactttga agatacggct gaaaaagacc
     6601 gtttcaagac cggtctaact cagctaggtt ttgattttaa aaaaaattat caagtcagcg
     6661 gttatcaaaa tatagagcct cttactcaag ctgaattggc tcggcggtgc ggatggtaaa
     6721 taaaaaatag ttttttaaca acctattgca aagtgaagct cagcctttgc agtatcaata
     6781 ttttccgagt gagaaaggaa agttggaata tcgtcaagtt aacaggattg atgatataaa
     6841 aaattgctac actcgtcctt gccaatgctc acacacaatt ttagggcgag tgtggatttt
     6901 aaaaggtgag aaatatggaa caaatcaaaa ttacagaaac aggaatggtc gttataagcg
     6961 ataaagcact caaaactttt gtgattgcag gacatttatc ggagaggtgg gaatttacct
     7021 ctaaatttaa aaaattagat gagccatcac ttgatgaaaa cggagatttg tttgagcctg
     7081 tatacgagtt gatgcttgaa gccaggtcga agggacaaat tagcataaca tcatcatatt
     7141 gtggcaaaaa tcacaaaaaa gacacagacg aaatcataaa agtattctcg tttatcgaag
     7201 ataacaagag aaatattttt gaaaaccttg gtattcgtgg ggtgcttgaa tgagcaatct
     7261 agttttatcg ttagacatct caacatctgg aacagggtgg gccttattta aaggctcaga
     7321 ccttatccag agtggtgtct taaaacataa gagtaaatcc tactttgagc gcggacgcta
     7381 tatggctagc caattaggac taatccagtc acgagcatta aaaaaatacg attgctattt
     7441 tagtacaatc gcagtcgaaa aaaattcagt tatggaacct aaccagcaat ccatgcttaa
     7501 aatcggtatt gttacaggaa ttatcttagg aagattaata gctgataacg tcgcctttat
     7561 aaatgtatca acgtggcgta agcactggaa gtttagctac aaagaccgct ctaaaaaagc
     7621 gatgaaagca caatcgaaag aaaaagctct cgaatattgc ggaaaaacag taaaagatga
     7681 tgaagcggat gctattttga ttggctcata ctatgtcaat caaggctatc ttgatgaatt
     7741 ggagacacat gactactact aaaaaacacg ttgtgagagt ttacaacaaa ggtattacag
     7801 cgacttacgc gatctatgac aaaaagctgt ttaaggagca cgagttcgca accaaaaacg
     7861 aagcgatgcg gtttattaga cagctagagt tagctaatga taagcgagcg acagaatatt
     7921 atttgagaga ggtagagaaa tgataccaaa ttttagaggg tttaacaaaa aaactaaaaa
     7981 aatgtatagc attgatggct ttaaatcaag tgaacgcaaa atatacagat gcagcttagc
     8041 agatgatgag tttcgctctg gtcgcttaga gacattccat tttgtagagg ataatcttgt
     8101 ggaaaagtgc aacagaccac ctgaacgatt ttatcgacaa aaacaagtac gcaaaagtga
     8161 caggcgactt atggcaaggc agtgatcaag tcattgctga gcagtgtttt ttaaaagtat
     8221 tagaggagat gcaaaaatga acattgaaga agcgaaagaa ttagtagata attcaaaatt
     8281 ttatggaaag actagcagtg ttataaaagc cgaggttcgc gacattatag accagttaaa
     8341 ccaaccaaaa ccagaagtac cgcagtgtgt ggcggattgg atagaagagt gcaaagaaga
     8401 agatttaaca ctatcacttg cctatgatgt tgatgctttt ggcgaagtgg cgaaatggct
     8461 ttatgacact aatgatagca caaacattga cctatttgcc caagcatggc tagcttatcc
     8521 aaatatcacc attgaaaaag agaagctgta tacagtggag atacccgatc caaatcatga
     8581 cgtgggcaca gttatattga gtaagaataa gcacagggaa gcttacattg caattgacta
     8641 caccggaagt tggaaggaaa gaaaagcaaa ccacctcacc gaatcagaaa tccgcaaaga
     8701 cttcgattgg gcttggcaat ttgcgaaaga ggtgactgaa tgactgaaga gttaggagtg
     8761 ttatatagcg aaaaatggca taagtattat ttatataaaa cttgcaggta tatatcttat
     8821 gttgataatc cacatcaggc tactaaatgc acccgcaaac aagcagaaca gtttccacag
     8881 tttaaatggg taccgctgac aaaattataa ccccacgcaa gcgctcaaga gcctgcaatg
     8941 gctctgtggg ggtggaccga aattaaaaaa tagaaacgag aacctcctta caccaaaaca
     9001 aatctaacgc agattatcgg tcatttgtta ttattcaagg cgctaatact gacatcgtac
     9061 gcctgtgtca aaaataaaaa aagaaagaga gggcttttct ccacaaaaca aaaagacgtc
     9121 cacacggaac gcccccttgg ttaaatttaa gcttaaataa attataccat attgggggct
     9181 ttcatgacgt tttttcctga gattgatatc caaaaaacaa aatccaatgc caagcgtaaa
     9241 ttgagagagt atccacgctg gcgaaggata gctaatgacg tagatactca aaaagttaca
     9301 gctacttact catttgagcc aagacaaccg catggagttc ctagcaaacc agttgagaga
     9361 ttggcgatta atcgtgtgtc ggcagaacag gagctggatg cgattgagag agcagtcaac
     9421 gggatatttg atccagagta tagattgata ctgattgata agtacttgct tacatatcca
     9481 aagactgatt gtgatattta tacaaatctt ggctacgaga aaagtcagta ttacaacatg
     9541 ctagataatg ctttgatgtc gttttcagag ctatacaaag agggggtatt acttatcgag
     9601 aaattggaaa aaagctggaa ttaatatgga ataattatgt actttatata taattattca
     9661 tgttattata gtactatcaa aataacaaga agagataacc attttaccaa ctgactattt
     9721 atttagtcgt caactttaac tactatcaaa cttgttattt tactaaagct tagtagcttt
     9781 gcgttaaagg tgttaaatta ctcggttaaa ccggtcgggt aagtcgcaaa tgtgaagaga
     9841 gttgttgaga cgtaaggaaa tgtcatgtgc acgcatctta tgatattgca ggttcgaatc
     9901 ctgccgtctc aatagtggtt atttcaacca ctagggcaat acagcgagag atgtagagtg
     9961 gagctatagc cgtttttgtg tagaccttat ggtattaatc gcgttcgatt cgtgatgggt
    10021 ctatcagcgt cggggagacg cgaccccaca ccctttaagg cagatacgtt ctgatgtgtg
    10081 ggtttttatg attaaccgca agtccaaaac caagggtcgc aaccttgctt gtggttagta
    10141 ggcttaatgg tcgtttatgc acgttcgatt cgtgcaaagc ctgttcgata aatagaagtg
    10201 tcccagaatg gggtaggcaa taggcttagc attcatttgc tatttatcta tggttaacca
    10261 attagtcacc aattgtggtg gctttttatt atgtaaaaaa gaaccacaac agtggctctt
    10321 atgcttgtaa tttaagttca agtgcttcag taaggacttg agagaagttg aggtttttat
    10381 cttcggctgc atcgtttaac cactcgggaa tagtcacgtt tttgcggact tttttggagt
    10441 gatatttttt catgtaggca atcatatcga tgccgattaa agcaatatca gaatcacgat
    10501 actgtccttt taaatcggaa acggagctag cctttggata gtcagtatag tcttcgagag
    10561 caaatcctaa gacttcgact gccatttcat aggcatgccc taagtcgttg ccgaaagtaa
    10621 tagcttcagg aatatctgga aaacgaacgc caattgaatt agtttcgttg tcgtgtgtaa
    10681 atacagctgg atataataac ataattcttt cctttctagg taaacaataa ccaagatggt
    10741 cagggcttat ttcagccctg ccttcttgag aatatcgtct tcggtgcctt ttggtatatc
    10801 acctttgtgc atgggcacga ttgtgatatt gcctgtttca aagtttttgt actttgcatg
    10861 gctaccgttt tgactgactt ttacaaagcc attctttttc aagagtttga tgatttctct
    10921 tgcggttctt ggcatattgc ttacctcact ttctatactt atattataca cataaataag
    10981 gtggatgtca ataggtatgc gcaaaaaata cgtatttttt attggaggtt agggtatgag
    11041 gccgcagaag ctaatcattg ctggaggtag acggatgaca gtcgactacg acgatagatc
    11101 agcagagtat cgtgattaca atcgtaatcg ctggaagtac gataagcaag tcaaacagtt
    11161 ctacaactca aaaatatgga gagagacaag taagcaagtc ttgttgcaaa acgattatgt
    11221 ctgtgcgatg tgtggtggag aagcgactat gactgaccat attgtctcaa ttaagaaaga
    11281 ttggaacaag agattagatc ggaacaactt gcaagcaagc tgtaaagctt gtaacgatag
    11341 taaagcgata cgagaaagac gtaaaaacaa ttactagaaa aacgggtgta aaaattaaca
    11401 acagtacgtt aatattcgga aactaccccc ttcattttta aacggggcta tattgttcgg
    11461 aaacttaaga acgcgccctt ttccgtgcaa aaaattccct ttttgaaatt tttaaaactg
    11521 taaagttcgt gtaaaggagg tcttatggga agaaatttaa agctagtcga aacgacaaaa
    11581 aagcatctta cgaaagaaga aaaaatagtg cgagaaaccg cgcaaaataa ggcgtctgac
    11641 ggtcttaaaa agttgcaaaa gacacctcct gaacacttta ataatgtagc taagtacgag
    11701 tataggagaa tcatagaaga ccttcaaaac ctacccctaa gaaatctcga tagagggcta
    11761 ttagagttat attgcacatg gtatgctatc tacaaagaga caagcaaaaa attggatgag
    11821 gttggttatt ttacgaacga tccagataaa ggattgatac caagccctct tattttaacg
    11881 ttggaaaaag ccgctgcgaa cattagaagt agcgcaagtc aacttgggct aacggtagat
    11941 agtcgcatga agatgtttat accgaaagaa gaggaaaaac ctaaaagtat ttttgataaa
    12001 tttggaggtt aaaaagaaag gaggtcatga caatagaata cgattattca gcaatcagcg
    12061 acatctataa agatgacgct ttttattatg caaaaatggt cgttgacgaa cagataaaat
    12121 caagtaagaa ggtatttaaa gcttgtttaa gacatttgaa tgacctcaaa aaaatagatg
    12181 gtgataattt taaattcatc tatttaccag aaaaagcagc tgatccaatt aacttcatcg
    12241 agattttgcc agatgttaaa acggggaaac cttatccgtt ggcgatgttt cagaagttta
    12301 ttatcgggaa tttatatgga tggcgaaaga aaacagatca ttccttgaga cgctttagaa
    12361 aagctatgat ttcggttgct cgtaaaaacg gcaaaacaat tctgatagct ggtatcttgc
    12421 tttatgaatt tttgtttggc cataacccat ctatgagcag acaattgttt tgtactgcaa
    12481 atgatagaac gcaggctaaa atcgcttggg atatggcaaa gaagcagtta tcatctctca
    12541 gagcgaagga cgctgatgtc agaaaggcta caaaaattgt ccgtgatgag cttaaaaact
    12601 tacatgacga atcatatatc agagcactta gtcgagatac tggggctgta gatggatttg
    12661 agccttatgt cggcgtctta gatgagttcg cagcatcaaa gactaatgaa atgctagagc
    12721 ttttagaatc tggtcaaggt cagcttgata acccgtttat cttgattatt tcgacggcag
    12781 ggatggattt gaatgtccct atgcacacaa ttgagtatcc atacatcact aagatactag
    12841 atggagaaat tgtagatgat ggctattttg gatatgtcgc agaacaagat aatgaagagg
    12901 aaattaagga tgaatcaaac tggataaaat ccaatccaat ccttgaggtt aaaactttac
    12961 atgataagat tatggactat ttacgaactc gtagacgagt atctctcgaa acaggggaaa
    13021 ttaataaagt cctgattaaa aactttaata tgtggcgtca atctagcgag gaatcctata
    13081 ttgacaagca atcttgggaa cttgctcaga ttgataaacc agacaccaca aaacgcaggg
    13141 tttggttagg ggttgatgtt gggcgtgtga gtgacttatt tgctatcaca ccagttatta
    13201 tgatggatga ctactggtat cttgatagct tttcttttgt tgctaccaaa tatgggttaa
    13261 cagccaagga aaaacgagat ggcgtctcct atagcaatct ggaacgtcaa ggttattgcg
    13321 agataacaac acttgaaagc ggggttattg atgatgagcg tgtcctagaa aaaattgaag
    13381 agatggttta ctcaaacgac tgggaagtta atggaatttg ttttgatcct tatcaatttg
    13441 gaacattact tacaatgatt gaaaaaaggc atccagagtg gcctctgatt gaggttaatc
    13501 agacgacgat ggttttgaac atgccgacaa aacaattccg tgacgacctc aaaaaaggca
    13561 aaataaagca ctctggtaac cctttgctga ccatggctgt caacaatgcc tatatcaaga
    13621 ctgataataa tggcatgaga atcgacaaga ataagaatag caataagatt gacccccttg
    13681 atgctgctct tgatgggtat gcagtttgtt acttagagcc atttgatggt tctggttact
    13741 ggacaagtga aaagatttta ggaggagaga cgctattttg attggtttta ttttaaaaaa
    13801 catacataca ttaatcttgt tagttggatt agggctgtta atacacggtc tttttttgtt
    13861 tggtgataaa gttggtttta tcgcaagtgg ccttatatta attattttgg ctatctacgt
    13921 agatagcata ggaggaagaa aatgaataaa cgcattaaga aaaaacgcaa aatcgaacaa
    13981 gctattgtgt tattgattgc agaaaatgct atgcaagcag aagcaattaa gagtcaagac
    14041 agacaaattg cagtgctaac aggaatcgta caacacaacg ctatggcaac aaacgaagag
    14101 ttagcgactg ttaaagctgc tactttagat aaccaagtag ctatcaaggc aattggtgat
    14161 gatgttgact acattaagaa aaattataag cgcaagtggc gaacatagat gttaatggtt
    14221 tagaaaggag gtgagaaatt tatgagtttt ttccaacctt tgggaagttc aaaggtgtcc
    14281 tacgatgact acatatcgtc ggtgttagct ggtgatgttt ctcaacaata tcttggggtg
    14341 tcggcgttaa aaaacagcga cgtactaact gcgacatcca tcatagcagg agatattgcg
    14401 aggtttcctt tacttaaaaa agatattact ggagatatta ttcatgacga ggatattaat
    14461 tatcttttaa atgttaaatc cacaaataat gcgagtgcta ggacttggaa atttgctatg
    14521 gcagtcaatg ccattttgac cggcaactcc ttttctcgta tcttacgaga ccccaaaaca
    14581 ggtcaggctt tgcagtttca gttttacaga ccgtcagaga caacagttga ggaaacggat
    14641 aatcatgaaa tcatctacac ttttactgat actttaacag cgaaacaggt taaatgcttc
    14701 gctcacgatg tcattcactg gaaattcttt agccatgaca caattttggg taggtctcct
    14761 ttactgtctt tgggtgatga gatagaccta caaaaaggtg gtatcaatac tttaattaag
    14821 tttttcaagg atggtttttc gagtggcatt ctaaccatga agggcgctca attaagtgga
    14881 gaagcacggc aaagagcgcg acaagacttt gaaaaaatgc gagaaggttc tgttggtgga
    14941 agtccgctag tatttgatag cacaatggaa tatacaccgc tagaaattga tactaacgtt
    15001 ttgcaattaa tcacgagtaa caatttttca accgctcaga ttgccaaggc tttacgagta
    15061 ccaagctata aacttggcgt taatagccct aaccaatctg tagctcaact gatggaggac
    15121 tatgtcacaa acgacttgcc tttttatttt gacgcgatca caagcgaatt agggcttaaa
    15181 acattgaatg acaaggacag acgtctgtat catatcgaat ttgacacacg gagtgtcaca
    15241 ggtcgtaatg ttgatgagat tgtcaaattg gttaacaacc aaatcttgac acctaatcaa
    15301 ggacttgttg aacttggtaa gcaaaaatct acagatccta atatggatag ataccagtct
    15361 agcctaaact atgtcttttt agacaaaaaa gaagaatatc aggacaaggt tggtatcaaa
    15421 gggaaaggag gtgaggtaaa tgccaaagag gataaatctt aaaggtccgc taatttcaaa
    15481 taactctcaa gaagtttacg actattacgg aatggaagcc gttagtgcaa aaagtatttt
    15541 tgataacctt cccgaagaca atagcgatat tatcttggaa gttaattcaa atggtggtct
    15601 agtcactgtc ggaagcgaaa tctatacggc tttgcgaaat tataaaggaa aagtaaccgc
    15661 agaaatcact ggtatggctg caagtgcagc atcagtggct gttatgggag ctgataaagt
    15721 cgtcatgagt ccaaccgctc agatgatggt gcacaaagca ctttttaatt gggtgtctgg
    15781 taatagcgat gacctagaca aagcttctaa cgctctgaaa tctagcgata aagctatcgt
    15841 gaatgcctat gtcgcaaaaa cagggctatc ggaagatgaa atcatggatt tgatgcgaaa
    15901 tgaaacgttt atgtccgctc aagatgccgt agaaaaaggt tttgctgacg aagtgatgtc
    15961 gtttgaagca gtcgctagca ttgataattc aatgttgcca caagcggtaa ttgatgatta
    16021 ttacgcaagt agaaataaac gtaaaaaaga aattagcaat atgctactag aaatcgaaaa
    16081 agaagaaatt ttacaagggc tataggctct ttttttattg gaggaattta tgttcgaaga
    16141 aaaaattaaa gaaattaaag cgaatatcgc tagcttaaac caaacaattg ctacgaaaac
    16201 agcagaagta aaaaatgctt tggaatcaga tgatcttgaa actgctcgct caattaaagc
    16261 agaagttgaa gaaactaaag cgagcctagc agaagcagaa aatgacttaa aattatatga
    16321 atctagcatt gaaaaaggcg gtgcagaaaa tactggagga aaagaagtgc cacaagaaac
    16381 taaaacatac cgcgaaagcg ttaacgaatt tattcgttca aaaggaaaag cggtcaatga
    16441 agatttacgc tttgaaggta aaaatgaggt gcttatttcg atgaatgagg taacaccagt
    16501 tgctcctaaa acagacggag ttaaaaaggc agacgtgaaa ccagtttcaa gcgaagaaat
    16561 cttatacaca ccagctcgtg aggttaagac agttgttgat ttgaaacaat ttactagtat
    16621 ccacccagct aaaaaagcat cagggaaatg gccggtattg caacgtgcga ctgacaaaat
    16681 ggttagcgtt gaagaattgg aaaaaaatcc taagctcgca aaaccacaat ttaaagaagt
    16741 taaatgggag gtcaagacat atcgtggagc tatcccgttg tctcaagagt caattgacga
    16801 cgcagatgta gatttggttg gtattgttgc tgaaacaatc ggtcaaatga aagtcaatac
    16861 aaccaatggt gcaattgctg aagttctcaa gaaattcgaa tctaaggaag taaaagactt
    16921 agacgaaatc aagaaacttc tcaacgttga tttagatcct gcttataacg tgtcatttat
    16981 tgtatctcaa agtttctatc aaacaatgga tacattaaag gataagaatg gtcgttacct
    17041 acttcaagat tcaatcactt ccgtttctgg gaaagtattt cttggtaaac ctgtttttgt
    17101 actagctgat gaggttttag gtgaaaataa agcatttgtc ggagatttca aacgtggtgt
    17161 attattcgcc gatcgtaaag acttagggct tcgttgggca gataacgaaa tctatggtca
    17221 atacttacaa gcggttcttc gctttggcgt ttctaaggtt gatgaaaaag ctggatacta
    17281 cgttacgttt acacccagtg aaagcctctg agaaagaggc gaaaccaact agtaaaagca
    17341 ctgtagaaga aatcaaacgc tatttaacaa gtcaaggaat tgattttagt ggtaagacct
    17401 tgaaatcaga tttacttgca ttagcaggcg ttgaagaggt atagctatgg ctgtatcgaa
    17461 agagttatta gacagtgtaa aactctattg taaaattgac tttgattttg aggacgacat
    17521 catcaaagaa atgattgaat cggcacagga acaaatctgt tttgcaatag atgatggctc
    17581 aaccgcagac acgtttaaag atagcgctaa atttgcttta gctgtcaaaa agcaagtcaa
    17641 ggaggagtac gaccatcgcg gtttgtctgc ggatagtttt cgctatccgt tggcaaatgg
    17701 cgttttaaat atcatccatc aattacgatt gcggggtgat gattcatgat tacacgcaaa
    17761 atgaacacaa ggataacgat tttcagccag tcgggtggtc agaatgacga tggcgaagtt
    17821 gtatctgctg tcagaaaaga cgtctataca tgttgggcag aagtgctgaa aactcaatta
    17881 agagacttta attacaaatc aaagtttcaa aacgctagcg atttgccaac aaataaggat
    17941 acaaaggtct ttttgattag gtataatcct aagttatcta tcgataacac gatgtttgtt
    18001 gaatttaaca atcgtatcta taagatagac aaaatcgaat ctgacgaatc tagcaaagat
    18061 attactatga ttagtggagt aagcatgtca tgacaaaagg tttggatgaa attttagcta
    18121 atctcacaaa gcttgaagta aaagctccta aaacagcgaa atcagcagta actgaagttg
    18181 ctaaggagtt tgaaaaagca cttaaagcaa atactcctgt ttatgagatt gaaacagaag
    18241 aaagactgca agaagatact gttatcagtg gttttaaagg agctaatgtc ggtattgttt
    18301 caaaagaaat cggttatggt aaagcaactg gttggcgtgc tcactatcct aacgatggga
    18361 cgatttatca acgcggtcag gactttaagg aaaaaacaat caatcagatg acaccaaaag
    18421 ctaagcagat ttacgctgaa aaagtgaagg agggactagg actttgattg ctgaaacgac
    18481 agcttataaa ttattaagta acgataagac actaaatgag ctgttggata agctcagagg
    18541 tggttctttt aaaaatggat ttaagcaagg catatttacc tatgacattc cagacaaccc
    18601 tattgattta cgcaaagcag agttagcgcc ttttatgcga attaacacaa cgctagatgg
    18661 tcctgctgat tatgctgatg atgagatact ttgtaatgag caacgtatca ccatcaattt
    18721 ttggtgcaaa acagcttcag aagctgacca aattagcaaa tgtatagatg atattttaaa
    18781 aaaaggcggt tttgaaagat acaccgcaaa cgaaaaacca agatataaag atagcgatat
    18841 tgacttactc atgaatgtaa ggaaataccg ctattttgat ttttattaaa aagaaagagg
    18901 acaaaatggg aaaagtaaaa tttggattac gtgattttta ctacggagtg ttagatgaca
    18961 aagacgaggt tacggataaa aagaagggta ttaaacatct acctggaatg aaatccgcaa
    19021 aacttgatat tactaacgaa ttagtgacag tagctgctga tgatggacct tacgttgtat
    19081 tatcaggtgg aatcagcgag acaaaacttg aaatcgagtt gttagattta acatcagatg
    19141 cacgcaaaga tttctttgga atcacagtag aaaaaggtgt tgagaagtac aataaatcac
    19201 tcacgccaaa agacgtcact tgtgtgttcc gcacaagcga tgaaaacggt aaggcgattt
    19261 gggtcggact gcttaaaggg aaatttaatc tacctggaat ggaggcgcaa acaaaagaag
    19321 gcaccccaga ccctaaaccg gattctgtta caggtaactt cgttgctcga ggcaaagatg
    19381 gagatgttat tttgattggt cgcgaggatg caagtgattt taaaatcaat gattttttaa
    19441 aatgtgtgtt taatggttgt caagatgcgg atatcaaaga aataccaatt gcggaacaag
    19501 cacttttgcc tatgtaattt ttaaggtcgc atttaagctg cgacctttta tttttgataa
    19561 ggagtagata tgtacgaaat taccttaaaa aaaggcggtg ttgataagga atttaaaaaa
    19621 gactttatca atgtcgaaga taatttgttg gctattgagc atcaagtcag acagagtgct
    19681 ctgtttggtg atgacaaacg ccgcttagag tctaaagccc acagaaagtt aaacgaatct
    19741 tacttgcaaa tgtttgttga catgtatgca ggtcaattta cagttgatga tttaaagcaa
    19801 tctgacatga gtgttttaaa tacacttaat gacctgtaca ttgcagcact cggtggagag
    19861 caagaggaag aacaagccga aaaaaaggaa caataacacc acaagaagca aaggaaaact
    19921 tactattgtg gatacaaagc ctattaaaaa acgggtacac cattttagat ataaaaaaaa
    19981 tgcgcttatc tgatatcgaa ctgatggtac aagcgctaga aattgaaagt gtcgaaaaag
    20041 aagaagtgat tgaaacgacc ttggataagg cattcccgtt cctttttggc tagaaaggag
    20101 gctaaatggg gaatataggt gatttagtgg caacagccac attagacata tcacccttta
    20161 tgtcaaatac aagaaactta aagacctata tgaaaggctt agacaactcg ttaaaagctg
    20221 ttgagaatag ttttcgcgga cacggcggac gaattaaggg gcttaaagca gtctatactg
    20281 aaacaggcag tgccttaaaa ggctatcaag acttgttaaa gactcaatct caaaagtata
    20341 gcgacttaaa aagagaaatt ggcgatgtca ataatgctac tgctgaacaa aagcaaaaac
    20401 ttattggcgc aaaatcagca atgttggata ccgctgcgaa agtcacggaa ttgcaaaaca
    20461 gattgcgagc tttagctaca gaaactagtg tctttactcg ttttggtaaa gcggctgaaa
    20521 gagtgggcgg caagatgaag tcgtttggcg attcggtagc tggagttggt gctgcattta
    20581 caagaggagt tacggctccg attgttgcgg gcgcagggta tgcgattaaa gcagctgttg
    20641 actatgagtc tgcgttcgct ggtgttaaaa agacggtaga tgaaacggcg acagtatctt
    20701 atgcgaagct atcacagggt attaggcaaa tggccaaaga gttgccagct agtgcggtag
    20761 aaatagctca cgttgctgaa gctgcagggc agttaggtgt taaaacggga gacatcctta
    20821 gtttttctcg tacaatgatt gatttaggag aatctaccaa tctatctgca gaagaagcag
    20881 cgacatctat tgctaaaata gcaaacatta caggactagc atcatcagag tattctcgtt
    20941 ttggaagtgc tgttgtcgca ttaggtaaca attttgcaac aaccgaaaaa gacatcgtag
    21001 caatgacaaa ccgtattgcc gcatctggta agcttgcagg cctgacaaat caagaaatgc
    21061 ttgccttagc aactgctatg tcaagcgttg gtatcgaggc agaagctggt ggcacagcga
    21121 tgactcagtc gttatcagct attgagcgtg cagtatcggc aggtggtgaa gatttaacta
    21181 agtttgctca aatcgctaat atgtcatccg ccgattttgc taaagcatgg aaagaaaaac
    21241 caatcgtcgc tttgcaggag tttattaagg ggcttgggca acttgataaa aaaggcgaaa
    21301 gtgccacaaa agtgctagat gaactcggtt taagtggtat ccgccagtct aacatgctta
    21361 aatccttagg tttagcatct gaaacattag gcaaggctct tggaatctct aacaaagctt
    21421 ggaaagaaaa cacggcgttg actgacgaag ctaacaaacg ttacgagaca acagagtcta
    21481 agctaaaaat gcttaaaaac gaaatcaacg atgtagccat tgaatttggt ggaccattag
    21541 ttgacgcatt gagaaacgga ctcgaagcag ggaagccaat catccaaatg gcggctgact
    21601 tagctaagca attcaactcg cttgataaag agcaacaaca gcaaattgtt aagtgggggc
    21661 ttattgctgc cgccgctggt ccagctttgt ctatctttga taaaggtgtt ggcattattg
    21721 gcagtactat tcaaggtatt gggaaaatga gccaagggtt ggaggcttta tctggttggt
    21781 taaggacatt taaatctggt gcagttgcag ctagtgttgg tgctgaggct gcagcaacct
    21841 ctatgggggg tatggctgga gcggttgcct tattaagtaa cccagtaacg tggggtgttt
    21901 tgctaggtgg tgcagcgatc gtgggtatag gcttaattgc tgatagcatg tataaagccc
    21961 aaaaacgcac agaagagtgg gggactgctg tttctcagac ggaagctgtt gctttgagca
    22021 actttaagaa aaaagttgac gaaaccaaca cttctttgca aatgttcgag gcaggcgcaa
    22081 gtagtgttaa gaaagtgacg gaagcttttg acgacttggt cggaagtatt gaaaaattag
    22141 ctcaatcaaa attagacaag aatataaact tagctaaaaa attagggttg tcagaagaaa
    22201 ccataaatgt tttgaaatcc aagactgaag ccgttgttaa taatgttaaa gggatgaacg
    22261 ctcagataaa agctattatg gaaaaacata atggcgacat gagtaagctt tctgagacgg
    22321 aaaaagagtt agtgttaaga aatcaaagag aaatgattag cacacagctt gatttgatga
    22381 agttttctgc gtcagaaaag aaagctttaa cagctgcttt aaataacgag ttagatgctc
    22441 ttaatgctag acaacttgaa aaagtatctg aaaataccgt caagatgctt gataaagaaa
    22501 attcggcata taagacaaaa aaagcagagt taaaagaaat tttaaaacaa tttggaagtg
    22561 acactagtaa attgagtgct gaagagttgg ccgctagaca agaagttttg aatagactta
    22621 ccgaactaaa catgcagcac aacctgaaga ctaaagcttt aagtgatcag tatcttgcta
    22681 tccagaggga gcgagtccaa cggttaaaag aatctggtaa gagccaagaa gaaattcatc
    22741 gggggataag ccaaatggcg tctgacatgg cacaaaagct tggcattagc tatgacgacg
    22801 cttaccgtaa actagcttat tacactgaga aatctggtga aacgttgaaa gttttatcac
    22861 gtaataccgc taatgccacg gcagaggttg cagctgctaa cgctcaatgg gatagtctgt
    22921 ttacgagtga taacccacaa caaagtttaa atgaattgtt atcaacagca gaaggttgga
    22981 acagttttga aatcatggtt aagaatgctg atgttgaacc gacaggtaga gccgcacttg
    23041 ctgaaatgct agtagctggc ggccaatggg aaaatatgac gttagagcag aagaagttgg
    23101 ttgttgatgg tcaacaagcg atgattgaaa tctttgatag caaggaattg ttagcgcagt
    23161 ggcaagcatt gacgccagaa gaaaaggttt tgctagcgaa aaacttaaca caagaaccaa
    23221 ctatgtcggc tcaacaagcc cttgatagtg ttaagcaaac agtacctgct gatgttaatg
    23281 caagtgataa aacagcaggc gaaactcaat cagcacaaaa caagattgac aatgttaagc
    23341 agaaagcgcc agccgatgtg aaggcatcgg ataagactgg accagatgtt gcaagtgcca
    23401 atagggcagt caatagtcct aaacaaaata gtccagctgt tatcagagca caagataacg
    23461 caagcggcgt tgcagaaaac gtaatatggt cattggctag aatcccaaga agtgttacaa
    23521 caaccattac aacgtttgtc cgtaaaattt ttggacatga aaaaggaact gatttccacc
    23581 ctggtggctt agccatggtc aatgaccaaa aaggaccatt atacagagag ttggttactt
    23641 tgccaactgg ggaatcgttt attccaagtg gtcgaaatgt tatccttcct ctgccgagag
    23701 gatctaaagt cttaaaggcc agccgcacaa aacagttatt cccgcactat gcaaacggaa
    23761 taggttttga cgacaccaaa attgccaatc tagcaacgcg tttaaaaaat gtgcatgata
    23821 aagggcaagt aattgtcaat aatgaccctc aaatagcaga attgattaaa ctgcttaaag
    23881 acagagatga cagaaatgtc acaaataact ataccttaaa cgctactaat agcagtagtt
    23941 cgggagatat gtttagccaa gagaacatga gacgactaat cagagaattg gcatactaca
    24001 caagcggaga aggaggaagg ttagcttaat gcgatacatt gaatttaatg gcattaaaag
    24061 caatgattta ggtttgttgc tagaacgcga gcggtctgtt aagtcaacga gcaacgatgt
    24121 caatttaata gaagtcgatg gtcgtgacgg cgtacttatt aaagacaacg aacgcttaaa
    24181 agcggttgag caagactttc ctttttcgct ggttggtgac gtggttgtca atcagcaaaa
    24241 aataagcgag tggttgcacg tcaaaggttg gcatgactta gctttgtctt gggacaagga
    24301 ctatatctat cgggctagtg ttgtcaatct ttttgagata gacgagattc taaaacagtt
    24361 cggcagactg aaaatcaact ttttaattca tcctatcaaa tacttaaaaa c
//
LOCUS       DAUDYX010000029        24026 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.029, whole genome shotgun sequence.
ACCESSION   DAUDYX010000029 DAUDYX010000000
VERSION     DAUDYX010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24026
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.029"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            150..629
                     /locus_tag="VHD02_001143"
     CDS             150..629
                     /locus_tag="VHD02_001143"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEQ5497759.1"
                     /translation="MNTNVNNAQVYRENYILALNNVDNKTEYEKFHADSLFNLGLTMY
                     SREYSFKSAIYNGFACSHIDDNLSLHPEQMRVLNLIKDNNGLIFSAPTSFGKTFVVFE
                     YIAREQPKNVVLIVPTLALVDEYKQKILKKYRDSFLEYKIYTSIDIEKEYDFNLKIA"
     gene            953..1267
                     /locus_tag="VHD02_001144"
     CDS             953..1267
                     /locus_tag="VHD02_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEQ5497760.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            1283..1669
                     /locus_tag="VHD02_001145"
     CDS             1283..1669
                     /locus_tag="VHD02_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEQ5497761.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1698..3083
                     /locus_tag="VHD02_001146"
     CDS             1698..3083
                     /locus_tag="VHD02_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEQ5497762.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            3086..3238
                     /locus_tag="VHD02_001147"
     CDS             3086..3238
                     /locus_tag="VHD02_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEQ5497763.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            3261..4466
                     /locus_tag="VHD02_001148"
     CDS             3261..4466
                     /locus_tag="VHD02_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEQ5497764.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPDHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            4509..4730
                     /locus_tag="VHD02_001149"
     CDS             4509..4730
                     /locus_tag="VHD02_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497765.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            4847..5344
                     /locus_tag="VHD02_001150"
     CDS             4847..5344
                     /locus_tag="VHD02_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEQ5497766.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            5319..5825
                     /locus_tag="VHD02_001151"
     CDS             5319..5825
                     /locus_tag="VHD02_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEQ5497767.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            5809..8256
                     /locus_tag="VHD02_001152"
     CDS             5809..8256
                     /locus_tag="VHD02_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEQ5497768.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            8259..10436
                     /locus_tag="VHD02_001153"
     CDS             8259..10436
                     /locus_tag="VHD02_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEQ5497769.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            10433..11434
                     /locus_tag="VHD02_001154"
     CDS             10433..11434
                     /locus_tag="VHD02_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEQ5497770.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            11431..12363
                     /locus_tag="VHD02_001155"
     CDS             11431..12363
                     /locus_tag="VHD02_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEQ5497771.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            12608..12724
                     /locus_tag="VHD02_001156"
     CDS             12608..12724
                     /locus_tag="VHD02_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEQ5497772.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            12740..14659
                     /gene="tet(M)"
                     /locus_tag="VHD02_001157"
     CDS             12740..14659
                     /gene="tet(M)"
                     /locus_tag="VHD02_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEQ5497773.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            14778..14945
                     /locus_tag="VHD02_001158"
     CDS             14778..14945
                     /locus_tag="VHD02_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEQ5497774.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(15005..15142)
                     /locus_tag="VHD02_001159"
                     /pseudo
     CDS             complement(15005..15142)
                     /locus_tag="VHD02_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            15558..15641
                     /locus_tag="VHD02_001160"
     CDS             15558..15641
                     /locus_tag="VHD02_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEQ5497775.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            15766..16503
                     /gene="erm(B)"
                     /locus_tag="VHD02_001161"
     CDS             15766..16503
                     /gene="erm(B)"
                     /locus_tag="VHD02_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEQ5497776.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            16858..17406
                     /locus_tag="VHD02_001162"
     CDS             16858..17406
                     /locus_tag="VHD02_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002402802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEQ5497777.1"
                     /translation="MIFGYARVSTDDQNLSLQIDALTHYGIDKLFQEKVTGAKKDRPQ
                     LEEMINLLREGVVIYKLDRISRSTKHLIELSELFEELSVNFISIQDNVDTSTSMGRFF
                     FRVMASLAELERDIIIERTNSGLKAARVRGKKGGRPSKGKLSIDLALKMYDSKEYSIR
                     QILDASKLSKTTFYRYLNKRNA"
     gene            17410..20328
                     /locus_tag="VHD02_001163"
     CDS             17410..20328
                     /locus_tag="VHD02_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HEQ5497778.1"
                     /translation="MAMKRILTTSQREQLLSVDHLSEEDFKAYFSFSDYDLEVINQHR
                     GKVNKLGFAIQLCLARYPGCSLSNWPIKSTRLTSYVSRQLHLDAIDLNSYDHRNTRAN
                     HFNEILEVFNYHRFGSANTQKQLIEYLIELALENDDSIYLMKKTIDFLTRKRIIFPSI
                     ATLEDIISRCRDKAENNLFSILLCSLTDIQIEKLESLFQIYEETKITKLAWLKDIPGK
                     ANPESFMSICKKVEVIASMGLGTINVSHINRNRFLQLARLGENYDAYDFSRFELEKRY
                     SLLIAFLVNHHQYLIDQLIEINDRILASIKRKGTRDSQEQLKEKGKLATKKLEHYASL
                     IDALHFAKDNDSNPFDEIERIMPWEDLVQDGEEAKKITGNKNHGYLEMVRNKANYLRR
                     YTPMLLRTLSFKATPAANPVLMALTQLTDLHNSGKRKIPADTSTDFVSKKWKSLVRPE
                     EGKIDRSYYELVAFTELKNNIRSGDISVEGSMIHRNIDDYLVDLSACIDSETIPDTFE
                     DYLKDREIILDLQLQFYSTVDKRISRANLKKLEKVTPSEAEIYRKKLYSIIPKIRLSD
                     LLIEVDSWTNFSQEFSHDSTGKPPSEQERKIIFAALLGLGMNIGLEKMAQSTPGISYS
                     QLANAKQWRFYKEALTRAQSVLVNYQLKLPVADFWGEGKTTASDGMRVPVGVSALKSD
                     VNPHYKSMEKGATMIRSINDRHTTHHIEVASTNTREATHTLDGLLYHETDLDIEEHFT
                     DTNGYSDQVFGMTALLGFDFEPRIRNIKKSQLFSIKSPSYYPNLSEDISGKINVKIIE
                     ENYDEIKRIAYSIQTGKVSSSLLLGKLGSYARKNRVALALRELGRIEKSIFMIDYITD
                     SELRRRITHGLNKTEAINALARELFFGRRGKFMERDIRRQLQSASALNVLINAISIWN
                     AVYLQAAYNYLVKIDPEVTKYMKHVSPINWEHITFLGEYKFDLLSIPKHLRELNIKNK
                     "
     gene            complement(20402..20623)
                     /locus_tag="VHD02_001164"
                     /pseudo
     CDS             complement(20402..20623)
                     /locus_tag="VHD02_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            21128..21550
                     /locus_tag="VHD02_001165"
     CDS             21128..21550
                     /locus_tag="VHD02_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEQ5497779.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            21547..21777
                     /locus_tag="VHD02_001166"
     CDS             21547..21777
                     /locus_tag="VHD02_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ5497780.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            22238..22441
                     /locus_tag="VHD02_001167"
     CDS             22238..22441
                     /locus_tag="VHD02_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEQ5497781.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            22523..23740
                     /locus_tag="VHD02_001168"
     CDS             22523..23740
                     /locus_tag="VHD02_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEQ5497782.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFH
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
BASE COUNT         8041 a         4149 c         4819 g         7017 t
ORIGIN      
        1 aaaaatagaa aagtttttag atagatttaa tagcgttaat tctaataaaa tgttaggatt
       61 agagacaaaa tatctgttaa tttctttacc agtaattgat aaggaaaaat ttgttgattt
      121 tgcaatcaga atggcggtga ataaagctcg tgaatacgaa tgtaaataat gcacaggtgt
      181 atagggaaaa ttacatactt gcgttaaata atgtagataa taaaacggaa tatgaaaaat
      241 ttcatgcaga ttctttattt aatttgggat taacaatgta ttcaagagaa tattcattta
      301 agtcagctat ttataatggg tttgcttgta gtcatattga tgacaattta tcattacatc
      361 cagagcaaat gcgagtttta aatctaatta aagataataa tggtctcatc tttagtgcgc
      421 caacaagttt tggaaagacg tttgttgtat ttgaatatat agcaagagag cagcctaaaa
      481 atgttgtttt gattgtacct actttagcac tagttgatga gtacaaacaa aaaatactca
      541 aaaaatatag agactcgttt ttggagtata agatttatac gagcattgat attgaaaaag
      601 aatatgattt taatttaaaa atagcataaa aatctagtta tccgcataaa aactggactt
      661 atcacacttt atcaaggtca aaaccactca atttactact aatttactac ttatgaatga
      721 gctttgatac gacgatttat ccttgaaaag tgaagatata aagatacttc caataaaatt
      781 tgaatattta ataggtagac acttcaaaaa atgaggtgtc tattttttta cccgattttg
      841 aaaggaagtg aacttatgaa aacaaaaaat caagaatcaa aaggtcgttc cccactcttt
      901 aagaccatca aacattcatt cagccaataa aaaagaaagg ataggtaaaa atatggaact
      961 taaatttgtg attcccaaca tggaaaaaac attcggcaat ttagaatttg ctggcgagga
     1021 taaagtcgtt cagcgaagaa tcaacggacg gctaactgtc ttatcaagaa gctataatct
     1081 ctattctgat gttcaaagag cagatgatat tgtggtggtg cttcctgctg aagctggcga
     1141 aaaacatttc ggctttgagg aacgtgtgaa gttagtcaat ccacgtatta ccgcagaggg
     1201 ctacaaaatc ggcactcgtg gttttacaaa ttacctttta catgctgacg acatgataaa
     1261 agaataaaga aagagaggaa aaatgatgag attagcaaat ggcattgtat tagataaaga
     1321 cacgactttt ggagaattga aattctctgc tctacgtcgt gaagtgagaa tccaaaatga
     1381 agacgggtcg gtttcagatg aaatcaagga acgtacctat gacttaaaat ccaaaggaca
     1441 aggacgcatg attcaagtaa gtattcctgc cagcgtgcct ttgaaagagt ttgattataa
     1501 cgcacgggtg gaacttatca atcccattgc ggacaccgtt gctactgcca cctatcaagg
     1561 agcagatgtt gactggtata tcaaggcaga cgatattgtg ctgacaaagg attctagttc
     1621 attcaaagct caaccacaag caaagaaaga accgacacaa gacaaatagt cgctaggtag
     1681 aaaggagact ttttcgcatg aaacagcgtg gtaaaaggat tcgcccatct ggtaaagatt
     1741 tagtctttca ttttacgata gcgtcactcc tgcctgtttt cctgctggtt gtcggactgt
     1801 ttcatgtgaa gacaatccag cagatcaact ggcaggattt taacctatca caagcagata
     1861 agattgacat tccctattta attatcagtt tcagtgtcgc aattcttatc tgcttgctgg
     1921 tagcgtttgt attcaaacgg gttcgctatg atacggttaa acaactttac caccgtcaaa
     1981 aactggcaaa gatgatactt gaaaacaagt ggtatgaatc tgaacaggtc aaaacagagg
     2041 gtttctttaa agatagtgct ggtcgtacaa aggaaaagat aacctacttc cctaaaatgt
     2101 attatcgact taaaaatggc ttgatacaga tacgggtgga aatcacgctg ggaaaatatc
     2161 aagaccaact cttacacttg gaaaagaaat tagagagtgg cttgtactgt gagctgacgg
     2221 ataaagagtt aaaggattcc tatgtggaat atactttgct ctatgacacc atagccagtc
     2281 gtatttctat tgatgaagta gaagctaaag atggtaaact tcgcttaatg aaaaacgtat
     2341 ggtgggaata tgataagctc cctcatatgt tgattgctgg tggtacaggt ggcggtaaaa
     2401 cttactttat actgacactg attgaagcct tgcttcatac agattcaaaa ctgtatattc
     2461 ttgacccgaa aaatgctgac cttgcggact taggttctgt gatggcaaat gtctactata
     2521 gaaaagaaga cttgctttct tgcattgaaa cattctatga agaaatgatg aaacgtagtg
     2581 aggaaatgaa gcagatgaag aactataaga ctggcaaaaa ttatgcttac ttaggtctcc
     2641 cggcacactt cttaatcttt gatgaatacg tcgctttcat ggaaatgctg ggaacaaaag
     2701 aaaacaccgc agttatgaat aagctgaaac agattgtcat gttaggtcgt caagctggct
     2761 tctttctaat actggcttgt caacgtccag acgcaaaata tttaggcgac ggaatccgtg
     2821 atcagtttaa tttcagagtg gctttaggtc gtatgtctga aatgggctat ggcatgatgt
     2881 ttggcagtga cgtacaaaag gatttcttct taaagcgaat caaaggtcgt ggctatgttg
     2941 atgtaggaac aagtgtcata tcagagtttt atactcccct tgtaccaaaa ggatatgatt
     3001 tcttggagga aattaaaaag ttatccaaca gcagacagtc cacgcaggcg acgtgcgaag
     3061 cggaagtcgc aggtgtggac tgatcttgct ggctggtgtg gcaatagcca cgccagcact
     3121 taaccccccg tatctaacag gggggtacaa atcgacagga aacagtcaaa aaaacattag
     3181 aaaatccttt ggttacaagg gatttacaaa atttcagcgt atgtcaaatg ggctttaaaa
     3241 gttgacatac gcctttttga ttggagggat ttttactgaa tgaacaaact tggttacagc
     3301 atttaaaaga aaaacgcttg gcttatggac tatctcaaaa ccgtttagct gttgcgactg
     3361 gtattacaag gcagtatcta agcgatattg aaacaggaaa agtcaagcca tcagaggatt
     3421 tacagcagtc cctttgggaa gctctggaac gcttcaatcc cgacgctccc cttgaaatgc
     3481 tgtttgatta tgtaaggatt cgctttccga caacagacgt acagcaggtg gtcgaaaaca
     3541 tcttacaact gaaactgtcc tattttcttc atgaggacta tggtttctat tcttattcag
     3601 agcattatgc tttaggcgac atattcgtcc tttgctccca tgaactggac aaaggagttc
     3661 tggtggaatt gaaaggtcgt gggtgcagac aatttgaaag ctatcttctg gcacaacaaa
     3721 gaagctggta tgagttcttt atggacgttt tggtggctgg cggtgtgatg aaacgccttg
     3781 accttgccat taacgataag acagggattt tgaatatccc tgtactcact gaaaagtgcc
     3841 aacaggaaga atgtatctcc gtcttccgca gttttaaaag ctatcgcagt ggcgaactgg
     3901 tacgcaaaga ggaaaaggaa tgtatgggaa acaccctcta tatcggttca ttacaaagtg
     3961 aagtttattt ctgtatctat gaaaaggact acgagcagta caagaaaaat gatattccca
     4021 ttgaagacgc agaagtaaaa aaccgttttg agattcgatt gaaaaatgag cgtgcctatt
     4081 atgcagtccg tgatttactc gtctatgaca atccagatca taccgccttt aaaattatca
     4141 atcggtatat ccgttttgta gataaagacg attccaaacc tcgttctgat tggaaactga
     4201 atgaagaatg ggcttggttt attgggaaca atcgtgaacg attaaaacta accacaaaac
     4261 cagagcctta ctccttccaa aggacgctga actggctatc tcatcaagtt gccccgacct
     4321 taaaggttgc gattaaactt gatgaaatca accagacgca ggttgtaaaa gacattctcg
     4381 accatgcgaa actgacagac cgacacaagc agattttgaa gcaacagtca gtaaaagaac
     4441 aggacgtgat aacaacaaaa aaataactca aatacaaatt cattgaatat agagaggaga
     4501 acatttttat gaattttgga caaaaccttt ataactggtt tctatcaaac gctcaatcac
     4561 tggtgctttt agcaatcgtt gtgattggct tgtatcttgg cttcaagcgt gagtttagca
     4621 aactgattgg ctttttaatt attgcgatta ttgcggttgg cttagtcttc aacgctgctg
     4681 gagtaaaaga cattttacta gagctattca atcgcattat tggtgcttaa ataaaaccgt
     4741 tcttttgtgg aatataagtg gttttcttat gttccgcaaa ggaatggtac accaaacgaa
     4801 gtgcggtagg gatttttgaa tctctacaaa gaaaggacgt gaatatatgg acgatatgca
     4861 agtctatatt gcgaatttag gcaaatacaa tgagggcgaa ttggtcggtg cgtggtttac
     4921 ctttcccatt gactttgagg aagtcaaaga gaaaatcggc ttgaatgatg aatatgagga
     4981 atacgccatt catgactacg agttaccctt tacggttgac gaatacactt ccattggcga
     5041 actcaatcga ctatgggaaa tggtatcgga attacccgaa gaattacaat cggagctatc
     5101 tgctctgctc actcattttt caagcattga agaactaagc gaacatcaag aggatattat
     5161 cattcattcc gattgtgatg atatgtatga cgtggcacgc tactacattg aagaaacggg
     5221 tgctttaggc gaagtaccag ctagtcttca aaactatatt gattatcaag cctatggtcg
     5281 ggatttagac ctttcaggaa cgtttatctc aaccaatcat gggatttttg aaatcgtcta
     5341 ttaaatctgt cggtacatta ctactggcag attttctatt ttacggggtg gctcaatcag
     5401 ctacccctat tttttatgaa aggattgatt acatgaagaa aatacgaagc tataccagta
     5461 tctggtctgt ggaaaaggta ctgtattcta tcaatgattt tagacttccg tttcccataa
     5521 cctttacgca aatgacatgg tttgtcgtgt cactctttgc agtgatgata cttggcaact
     5581 tgccccctct ttccatgata gagggagcat ttctcaaata ctttgggatt cctgtggctt
     5641 tcacatggtt tatgtctaca aaaacttttg atggtaaaaa gccttatgga tttttgaagt
     5701 ctgtcattgc ttatgcactg cgaccaaagc tgacctatgc aggaaaaaaa gtaacgcttg
     5761 gcagaaacca gccacaagaa gccattacag cagttaggag tgaattttat ggcatatcca
     5821 attaaataca ttgaaaacaa tctcgtctgg aataaagacg gggaatgtta tgcttactat
     5881 gagcttgttc cttacaatta ctcatttcta agtccagaac agaaaataca agtgcatgat
     5941 tctttcagac agcttatcgc acaaaatcgt gatggcaaaa ttcatgcttt acaaatcagt
     6001 acagaatcca gcatacgttc tgcacaagag cgttccaaaa atgaagtcac tggcaagctc
     6061 aaagcggttg cctatgacaa aatcgaccaa cagacagacg ctttaatatc catgattggc
     6121 gaaaatcaag tgaactaccg tttctttatc ggctttaagt tgcttctcaa cgatcaggag
     6181 ttttctatga aaagtcttac cgttgaagca aaaaatgctt tgtctgattt tgtctatgat
     6241 gtgaaccata agctgatggg cgattttgtt agtatgagta atgatgaaat cctgcgtttt
     6301 cagaagatgg aaaagctctt agaaaataaa atctctcgtc gtttcaaaat ccgcaggtta
     6361 gataaggacg acttcggcta tctgattgaa cacctttacg gacagacagg cactgcctat
     6421 gaagagtatg agtaccatct atcaaagaaa aagctggata atgaaacgct gattaaatac
     6481 tatgacttga ttaagcctac tcgctgtttg gtggaagaaa aacagcgata tttgaaaatc
     6541 cagcaggaag atgaaaccgt ctatgtagct tactttacca ttaacagcat tgtcggagaa
     6601 ctggacttcc cgtcctctga aatcttctac taccagcaac agcaatttac attcccgatt
     6661 gatacgtcaa tgaatgtgga aattgtagcg aatcgtaaag ccctatctac tgtccgcaat
     6721 aaaaagaaag aactgaaaga cttggataac cacgcttggc aaagtgataa tgaaaccagc
     6781 tccaatgtgg cggaagctct ggaaagtgtg aatgagctgg aaaccaattt agaccaaagc
     6841 aaggaatcta tgtacaagct gtcttatgtg gtaagggtat cagcaaatga tcttgacgaa
     6901 ctcaaacgtc gttgtaatga agtgaaagat ttttatgacg atttaagcgt aaaactggta
     6961 cgaccatttg gggatatgct cggcttacat gaagaatttt tacctgccag caagcgttat
     7021 atgaatgatt atattcaata cgtgacctct gatttcctcg ctggtttagg ttttggtgct
     7081 actcaaatgc tgggggaaaa tgaggggatt tatgttggct acagcttaga tactggacgc
     7141 aatgtctatc tgaaacctgc tcttgccagt caaggggtta agggttcagt aaccaatgcg
     7201 ttagcgtcgg cttttgttgg ttcgctgggt ggtggtaaat cctttgcgaa taaccttatc
     7261 gtctattatg cggtgcttta tggggcacaa gcagtgattg tagacccaaa agcagaacgt
     7321 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt cactctgact
     7381 tctgatgaga aaaacaaagg cttacttgac ccttatgtga ttatgaaaaa tcccaaagat
     7441 tctgaatcac tggctattga tattctgaca ttccttacgg ggatttcctc tcgtgatggg
     7501 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtacgaggg
     7561 ttgatgaaag tgattgagga attacgggtt gagaatacgc cactaagtac cagtatagcc
     7621 gaccatatcg aaagttttac agactatgac tttgcacatt tattattcag taatggttat
     7681 gtggagcagt ctatcagctt agaaaaacaa ctgaacatta tacaggttgc ggacttggta
     7741 cttcccgaca aggaaacttc ctttgaggaa tataccacta tggagctttt atccgttgct
     7801 atgctgattg tcattagtac ctttgcttta gactttatcc atacagaccg aagcattttc
     7861 aagattgtag atttagacga agcatggagc tttttacagg tagcacaagg aaaaacacta
     7921 tctatgaagc tggttcgggc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
     7981 aatacagacg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgcattt
     8041 cgttccactg accttaacga gattaaaaag accttagcct tttttggtgt agacccagag
     8101 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
     8161 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
     8221 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atagtaaaca
     8281 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     8341 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     8401 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     8461 aactggattt ttatgtggat aatagctggg gctggcttcc gtggaactgg tcggacggga
     8521 ttggaaaaca ggtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtt
     8581 tgtatgtttc caatgcgaca ggttacttag tacaggaagc ctattcctta gacttcattt
     8641 ccgctacagc agattccatt ggtaagaata tgcagacctt agctggtgtg agtgcaaacg
     8701 gattttcaac agagggtttc tatgttggat tcctcttact cttgattttg gttcttgggg
     8761 tttatgttgc ctatacggga ctgataaaga gagaaaccac aaaggcaatt catgccatta
     8821 tgaattttgt gctggtgttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     8881 ttaaaaaaat caatgacttt tcatcagaca tcagtaatgc cagtttatca cttggcacga
     8941 agattgtcat gccccattcc gatagtcaag gcaaggacag cgtggactta atcagagata
     9001 gcctgttttc catacaggtt cagcaaccgt ggctactgct tcaatacaac agttcagaca
     9061 ttgaaagtat cggtattgac cgtgtggaaa gcctgctctc caccagccca gattccaaca
     9121 atggcgaaga cagagaaaaa attgttgcgg aagaaattga agacagaagc aataccaatc
     9181 taaccattac aaagaccatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     9241 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     9301 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     9361 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     9421 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     9481 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttca
     9541 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     9601 gtcgtgtgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     9661 aacttggacg ttccatgact actctagggg ctgggtctgc cattgttaca ggtaaaaaag
     9721 gacagtcggg ttcggggagt tctgcaagga cacaagcaga tcactcccga ccagacggaa
     9781 aggaaaaatc aacacttgga aaacgtatcg gtcaaaccat cggtacagta gctgatacca
     9841 aagacagaat ggtagacact gctagtggtt tgaaagaaca ggttaaagat ttgccgacca
     9901 atgcaagata tgcagtatat caaggaaaat ccaaagtaaa agagaatgtc cgtgatttaa
     9961 ccagtagtat ttctcaaacc aaagcggaca gagccagtgg acgcaaggaa cagcaggaac
    10021 aaaggcgaaa aaccattgcg aagcgtcgct ctgaaatgga acaggtcaaa cagaaaaaac
    10081 agcctgcttc ttctgttcat gaaagaccga ctacaagaca agaacaatat catgatgaac
    10141 agacctcaaa acagtctaat attcagactt catataagga atctcaacaa gccaaacaag
    10201 agcgtccagc agttaagtcc gatttttcaa gtccaaaagt ggaacgccaa ggcaataccg
    10261 ttcaagaaaa aaccgttcaa aagccagcaa cttcaaccac tacagcagat agaacttcac
    10321 aacgtccaat cacaaaagaa cgtccgtcta ctgttcaaag agtaccacta caaaatacaa
    10381 gaagtagacc accaatcaaa accgccacca ttaagaaagt cggtaagaaa ccatgaagtt
    10441 gaaaacttta gtgattggtg gttctggatt attcttgatg gtcttctcac tgcttctgtt
    10501 tgttgccatt ttattttcag atgaacagga cagcggaatt tccaatattc attatggagg
    10561 tgtgaatgtt tccgcagaag tgctggctca taagcctatg gtagaaaaat atgccaaaga
    10621 atatggcgtt gaagaatatg tcaacatact tcttgcgatt atacaggtgg aatcgggcgg
    10681 tactgcggaa gatgttatgc agtcctcgga atccctcggt cttccaccta attcattgag
    10741 tacagaagaa tccattaagc aaggtgtgaa gtatttcagt gaattattag ccagtagcga
    10801 aaggctcagt gtagatttag aatcggttat ccagtcctac aattatggtg gtggtttctt
    10861 agggtatgtg gctaatcgtg gaaataaata tacctttgaa ctggctcaaa gtttctcaaa
    10921 agagtattca ggtggcgaaa aagtgtctta ccccaatccc atagccatac ctatcaatgg
    10981 gggctggcga tacaactatg gcaatatgtt ttatgtgcaa ctggtaacgc agtatcttgt
    11041 cacaacagag tttgatgatg atacggtaca agccatcatg gacgaagcac tgaaatatga
    11101 gggctggcga tacgtttacg gtggagcttc cccgactact tcttttgatt gtagcggact
    11161 gacacaatgg acgtatggaa aagctggaat taacttacca cgaaccgcac aacagcaata
    11221 tgatgtgacc cagcatatcc cactatcgga agcacaagct ggcgatttgg ttttctttca
    11281 ttctacctat aacgctggct cttatattac tcatgttggg atataccttg gcaataaccg
    11341 tatgtttcat gcaggcgacc caatcggtta tgccgactta acaagcccct actggcaaca
    11401 gcatttagtg ggagcaggac gaatcaaaca atgagaaagg aagatttaat gatgaaattt
    11461 agaaaaaatc agaataaaga aaaacagata ccaaaggaaa agaaacctcg tgtctataag
    11521 gtcaatcctc ataaaaaggt tgtgattgcc ttgtgggtac ttttagggct tagtttcagc
    11581 tttgcgatat tcaagcactt tacagctata gatactcata ctattcacga aacaactatc
    11641 atagaaaagg aatacgttga tactcatcat gtagaaaatt ttgtagagaa ctttgcgaaa
    11701 gtctactatt catgggagca atccgataag tccattgata atcgaatgga aagtctaaaa
    11761 ggctatctga cagatgaact tcaagctctc aatgttgata cagtacgcaa agatattcct
    11821 gtatcgtctt ctgtaagagg atttcagata tggacggtag agccaactgg cgacaatgag
    11881 tttaatgtaa cctacagtgt agaccagctc attacagagg gagaaaatac aaagaccgtc
    11941 cactctgctt atatagtgag tgtctatgta gatggttctg gaaatatggt actggttaag
    12001 aatccgacca ttaccaacat acctaagaaa tcaagttata aaccaaaagc cattgaaagt
    12061 gaggggacgg ttgattccat tacaaccaat gaaatcaatg agtttttaac gacgttcttc
    12121 aagctctatc ctacagcgac agccagtgaa ctttcctact atgtgaatga cgggatatta
    12181 aaaccaatcg gaaaagagta catctttcaa gaactggtaa atcctattca caatcgtaag
    12241 gataatcaag tcacggtatc gctgacagtg gagtatatcg accagcagac caaagcaacg
    12301 caggtatctc aatttgattt ggtacttgaa aagaacggga gtaattggaa gattatagaa
    12361 taacaaatat tggtacatta ttacagctat tttgtaatca cgtactctct ttgataaaaa
    12421 attggagatt cctttacaaa tatgctctta cgtgctatta tttaagtatc tatttaaaag
    12481 gagttaataa atatgcggca aggtattctt aaataaactg tcaatttgat agtgggaaca
    12541 aataattgga tgtccttttt taggagggct tagttttttg tacccagttt aagaatacct
    12601 ttatcatgtg attctaaagt atccagagaa tatctgtatg ctttgtatac ctatggttat
    12661 gcataaaaat cccagtgata aaagtattta tcactgggat ttttatgccc ttttgggttt
    12721 ttgaatggag gaaaatcaca tgaaaattat taatattgga gttttagctc atgttgatgc
    12781 aggaaaaact accttaacag aaagcttatt atataacagt ggagcgatta cagaattagg
    12841 aagcgtggac aaaggtacaa cgaggacgga taatacgctt ttagaacgtc agagaggaat
    12901 tacaattcag acaggaataa cctcttttca gtgggaaaat acgaaggtga acatcataga
    12961 cacgccagga catatggatt tcttagcaga agtatatcgt tcattatcag ttttagatgg
    13021 ggcaattcta ctgatttctg caaaagatgg cgtacaagca caaactcgta tattatttca
    13081 tgcacttagg aaaatgggga ttcccacaat cttttttatc aataagattg accaaaatgg
    13141 aattgattta tcaacggttt atcaggatat taaagagaaa ctttctgccg aaattgtaat
    13201 caaacagaag gtagaactgt atcctaatat gtgtgtgacg aactttaccg aatctgaaca
    13261 atgggatacg gtaatagagg gaaacgatga ccttttagag aaatatatgt ccggtaaatc
    13321 attagaagca ttggaactcg aacaagagga aagcataaga tttcagaatt gttctctgtt
    13381 ccctctttat catggaagtg caaaaagtaa tatagggatt gataacctta tagaagttat
    13441 tactaataaa ttttattcat caacacatcg aggtccgtct gaactttgcg gaaatgtttt
    13501 caaaattgaa tatacaaaaa aaagacaacg tcttgcatat atacgccttt atagtggagt
    13561 actacattta cgagattcgg ttagagtatc agaaaaagaa aaaataaaag ttacagaaat
    13621 gtatacttca ataaatggtg aattatgtaa gattgataga gcttattctg gagaaattgt
    13681 tattttgcaa aatgagtttt tgaagttaaa tagtgttctt ggagatacaa aactattgcc
    13741 acagagaaaa aagattgaaa atccgcaccc tctactacaa acaactgttg aaccgagtaa
    13801 acctgaacag agagaaatgt tgcttgatgc ccttttggaa atctcagata gtgatccgct
    13861 tctacgatat tacgtggatt ctacgacaca tgaaattata ctttctttct tagggaaagt
    13921 acaaatggaa gtgattagtg cactgttgca agaaaagtat catgtggaga tagaactaaa
    13981 agagcctaca gtcatttata tggagagacc gttaaaaaat gcagaatata ccattcacat
    14041 cgaagtgccg ccaaatcctt tctgggcttc cattggttta tctgtatcac cgcttccgtt
    14101 gggaagtgga atgcagtatg agagctcggt ttctcttgga tacttaaatc aatcatttca
    14161 aaatgcagtt atggaaggga tacgctatgg ttgcgaacaa ggattatatg gttggaatgt
    14221 gacggattgt aaaatctgtt ttaagtacgg tttatactat agccctgtta gtactccagc
    14281 agattttcgg atgcttgctc ctattgtatt ggaacaagtc ttaaaaaaag ctggaacaga
    14341 attgttagag ccatatctta gttttaaaat ttatgcgcca caggaatatc tttcacgagc
    14401 atacaacgat gctcctaaat attgtgcgaa catcgtagac actcaattga aaaataatga
    14461 ggtcattctt agtggagaaa tccctgctcg gtgtattcaa gaatatcgta gtgatttaac
    14521 tttctttaca aatggacgta gtgtttgttt aacagagtta aaagggtacc atgttactac
    14581 cggtgaacct gtttgccagc cccgtcgtcc aaatagtcgg atagataaag tacgatatat
    14641 gttcaataaa ataacttagt gtattttatg ttgttatata aatatggttt cttgttaaat
    14701 aagatgaaat attttttaat aaagatttga attaaagtgt aaaggaggag atagttatta
    14761 taaactacaa gtggatattg tgtcctgtat gtggaaataa aacacgatta aagataaggg
    14821 aagatactga attaaaaaaa ttccccctct attgtccgaa atgcagacaa gaaaatttaa
    14881 ttgaaataaa gcagttcaaa gtaactgtga ttacagagcc agacgcaaag acgcagagcc
    14941 gataaaatga gattaataca atctcatttt atcggctctt tccgttatgt atggattctt
    15001 ttaattagtc ttcgatgttt cttgcttcgt tgataccgct ggctaaagat tccattaagg
    15061 atagttcttt gtctgtaaag ctatccatgt atttctctat ctgtaatcgt cgggtgcttt
    15121 ttaccaagtt attagcaggt aggggtcccg agcgcctacg aggaatttgt atcgataaga
    15181 aatagattta aaaatttcgc tgttattttg tacatttaac ttgacggtga catctctcta
    15241 ttgtgagtta ttagtggtac agttttcaac cgttttaatt ataaaaaagt ggtgcatttt
    15301 taaattggca caaacaggta acggttattg caggtgtatt tcttatctat gggtttaaca
    15361 tggattttat cattaaaatc atgagtattg tccgagagtg attggtcttg cgtatggtta
    15421 accctaaagt tatggaaata agacttagaa gcaaacttaa gagtgtgttg attgtgcatt
    15481 atcttaaaat tttgtataat aggaattgaa gttaaattag atgctaaaaa tttgtaatta
    15541 agaaggaggg attcgtcatg ttggtattcc aaatgcgtaa tgtagataaa acatctactg
    15601 ttttgaaaca gactaaaaac agtgattacg cagataaata aatacgttag attaattcct
    15661 accagtgact aatcttatga ctttttaaac agataactaa aattacaaac aaatcgttta
    15721 acttctgtat ttatttatag atgtaatcac ttcaggagtg attacatgaa caaaaatata
    15781 aaatattctc aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg
    15841 aatttaaaag aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg
    15901 aaactggcta aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac
    15961 ttatcgtcag aaaaattaaa actgaacatt cgtgtcactt taattcacca agatattcta
    16021 cagtttcaat tccctaacaa acagaggtat aaaattgttg ggaatattcc ttaccattta
    16081 agcacacaaa ttattaaaaa agtggttttt gaaagccatg cgtctgacat ctatctgatt
    16141 gttgaagaag gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg
    16201 cacactcaag tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa
    16261 ccaaaagtaa acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa
    16321 tattggaagc tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg
    16381 tttactaaaa atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc
    16441 gttacttatg agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa
    16501 taattctatg agtcgctttt gtaaatttgg aaagttacac gttactaaag ggaatgtaga
    16561 taaattatta ggtatactac tgacagcttc caaggagcta aaggggtccc gagcgcctac
    16621 gaggaatttg tatcgataag aaatagattt aaaaatttcg ctgttatttt gtacaataag
    16681 gataaatttg aatggtacca taaacgaccg tttatggtac cttttcattt tcctgctttt
    16741 tctaaatgtt ttttaagtaa atcaagtacc aaaatccgtt cctttttcat agttcctata
    16801 tagttatact taatgagtta tggtacattt aaattataaa attaaggagg tttttttatg
    16861 atttttggct atgctcgagt gagtacggat gatcaaaatc ttagtttaca aattgatgca
    16921 cttactcatt atggaattga taaattattt caagaaaaag taactggtgc gaaaaaagac
    16981 cgaccgcaat tagaagaaat gatcaaccta ctacgtgaag gagttgtcat ttacaagtta
    17041 gatcgaattt cacgatcaac taaacatttg attgaacttt ctgaattatt tgaagaactt
    17101 agtgtcaatt ttatatctat tcaagataac gtagatactt caacgtctat gggaagattc
    17161 tttttccgag ttatggctag tttagcagaa ctggaacggg atattattat tgaacgaact
    17221 aactctggtc ttaaggcagc cagagtccga ggaaaaaaag ggggccgtcc aagtaaaggt
    17281 aagctatcaa ttgatttagc tttaaaaatg tatgacagca aagagtattc tattcgtcaa
    17341 attcttgatg cctctaaatt aagcaaaaca accttttacc gttacctcaa taaaaggaat
    17401 gcttaagata tggctatgaa aagaatttta actacttcac agcgtgaaca acttctttct
    17461 gtagaccact tatcagaaga ggattttaaa gcgtatttta gtttttctga ttatgatctg
    17521 gaggttatta atcaacaccg tggaaaggtc aataaactag gatttgcgat acaactttgt
    17581 ttggcccggt atcctgggtg ttctttaagt aattggccga ttaaatcaac cagactaact
    17641 tcttatgtga gtcgacagct ccatcttgat gcaattgatt taaattcata tgatcataga
    17701 aatacacgtg caaatcactt caacgagatc ttagaagtat tcaactatca tcgattcggt
    17761 agtgctaata cacaaaaaca gttaatagaa tatttaattg aactagcttt agaaaatgat
    17821 gactctatct atctaatgaa aaaaacaatt gatttcttaa ctcgaaaaag aattattttt
    17881 ccatctatag ctacacttga agacattata agccgctgtc gagataaagc agaaaacaac
    17941 ttattttcaa tattactctg ttcattaaca gatatacaaa ttgaaaaact agagagtttg
    18001 tttcaaattt atgaagagac gaaaataact aaactcgctt ggctaaaaga cattccaggt
    18061 aaggcaaatc cagaaagctt tatgagtatt tgtaaaaaag tggaagtgat tgcttccatg
    18121 ggactcggga caattaatgt ctcccatatt aatcggaaca ggtttcttca gctagctaga
    18181 ctaggggaaa attatgatgc atatgacttc tcccgttttg agcttgaaaa aagatactct
    18241 ttacttattg cttttttagt caatcatcat caatatctga tcgatcaact gattgagatt
    18301 aatgaccgca ttttagcaag tattaaacgc aaagggacac gtgattcaca agaacagtta
    18361 aaagaaaaag gaaaattggc tactaaaaaa ttggaacatt atgcttcttt aattgatgct
    18421 cttcactttg caaaagataa tgatagtaat ccttttgacg aaattgaacg aatcatgcct
    18481 tgggaagatt tagtacaaga tggagaagaa gctaaaaaaa ttacaggtaa taaaaatcat
    18541 ggctatttag aaatggttcg aaataaagct aattacctcc gaagatacac gccaatgtta
    18601 ttgaggaccc tttcgttcaa agcaactccg gcagcaaatc cagtcctcat ggccctaact
    18661 caactaactg atttacacaa tagtggtaaa agaaaaatac cggcagatac ttctactgat
    18721 tttgtgagta aaaaatggaa aagccttgtt cggccagaag aggggaaaat agatcggtct
    18781 tactatgagt tagtagcttt caccgagcta aagaacaata ttcgatcagg agatatttca
    18841 gttgaaggaa gtatgatcca tcgaaatatt gatgattact tagttgattt atctgcttgt
    18901 attgattcag aaactattcc agacacgttt gaggactatt taaaggatcg ggaaataatt
    18961 ttagatttac agcttcaatt ttattcgaca gttgataaga gaatttcaag agcaaacctt
    19021 aaaaagttgg aaaaagttac acctagcgaa gcagaaatat atagaaaaaa actttattca
    19081 ataattccta agataaggct tagtgatctt ttaattgagg tggacagttg gaccaacttt
    19141 tcacaagaat ttagtcatga ttctacaggg aaaccgccga gtgaacaaga aagaaaaatt
    19201 atttttgctg ctttgctggg tttagggatg aatattggtc ttgaaaaaat ggcccaatca
    19261 actcctggaa tttcttattc tcagttagcc aatgccaaac aatggcgctt ttataaagaa
    19321 gctctgactc gtgctcaatc tgttttagtt aattatcagt taaagcttcc tgttgcagac
    19381 ttttggggtg aaggaaaaac cactgcttca gacggaatgc gcgtcccagt gggcgtctca
    19441 gctctaaaat ccgatgttaa tccacattac aaaagtatgg aaaaaggagc tacaatgatt
    19501 cgatcaataa atgataggca tacgactcat catatcgagg ttgcttcaac taatacaagg
    19561 gaagctactc atacccttga tggcctactt tatcatgaaa cagatctaga tattgaggaa
    19621 cattttactg atacaaatgg gtattctgat caggtgtttg gaatgaccgc attactaggc
    19681 tttgattttg aacctcgcat cagaaatata aaaaaatcac aattattttc tatcaaatca
    19741 ccttcctact accctaactt atcagaagat ataagcggaa aaatcaatgt aaaaattatt
    19801 gaagaaaact atgatgaaat taaacgaatc gcctattcga ttcaaacagg aaaagtatct
    19861 agttctttac tattaggaaa gctaggctca tacgcacgta agaatagagt agctcttgca
    19921 ctgagagaac taggtcgcat tgaaaagagc atttttatga tagattatat tacagatagt
    19981 gagctacggc gaaggatcac tcatggacta aataagacag aagcgattaa tgctttagct
    20041 agagaactat tttttgggcg acgcggaaaa tttatggagc gcgatattcg ccgacaactt
    20101 caaagtgcta gtgcgcttaa tgtgttaata aatgcaataa gtatatggaa cgccgtctac
    20161 ttacaagcag cttataatta tctcgtcaaa atagatcccg aagtaactaa gtatatgaag
    20221 catgtatctc ctattaattg ggagcatatc acttttcttg gagagtataa atttgacttg
    20281 ttatctattc ctaaacactt aagagaattg aatataaaaa ataaataggc cttgaaacat
    20341 tggtttagtg ggaatttgta ccccttatcg atacaaattc ccactaagcg ctcgggaccc
    20401 caggtaagaa aaattcatca acggaaacat gaagtaacga tacaaggtca taaagaactt
    20461 gtatgctggg gtgttgccct ttattttcaa tattagttaa gtaccgtggg tcaatttcaa
    20521 tcaatgctcc cacttgttca cgagttaaac ctcgtttcaa tcgagcttct ttaatggcta
    20581 aaccaaaggc tctaaaatca tatttatctt cttttttacg catagtagac cacctctata
    20641 cattttattg ttcctactga attaaaaaca ggtatagaaa aacgtgttat atggtttata
    20701 ggtttatatt taataaaaag cactactaaa cgccaataaa aaaaaccgtt atatggtagt
    20761 gctatttacg ctgttaaaat attgtatatt acttccaaat ggcggtttgt tggaggtcaa
    20821 cgtcgccatg aagtacatca tatacaataa atttccttac attgggttct tgtcaaaaaa
    20881 agtcgtctat ctgcaataga taagtacgtc caccaatgtg gttttataaa tcatatagat
    20941 agaataacag aagcatgtaa acagagaaat aaatctgttt atatgctttt ttggctattc
    21001 agaacttttt tacaaagttt atttatcagt aatgcaacaa atcccccttt cacattggga
    21061 ctaagagtga aaggagataa acgagcaagg ctcacttcct ttcctagaca gaaagggggt
    21121 gagaaacatg aaaccatctt cttttcagac cacaatagaa aatcagtttg actatatctg
    21181 taaacgtgct atggaagacg agcgaaagaa ttatatgctt tatctttcaa ggattgcaaa
    21241 gcgtgaggtg tccttttcgg atgttggcga ttatcttgtt agccagtttg cgacaacaga
    21301 taactattca actgactttc agatttttac actcaatggg ttatcagtag gcgttgaaaa
    21361 tgatttgttg agtgaagcat tacgtgagtt gccagacaag aaacgtgaaa ttctactgct
    21421 gttttacttt atggacatga gcgattcaga aattgcagac ctgttgaaat tgaaccgttc
    21481 tactgtctat cggcatagaa ccagtggact agccttaatt aaaaagttta tggaggaatt
    21541 tgaagaatga aaacacaata tcctatgatt ccctttcctc tcattgtaaa ggcaacagat
    21601 ggcgataccg aagcgattaa ccagattcta catcattaca gagggtacat aacgaagcgt
    21661 tccctacgac ttatgaaaga tgaatatggc aatcaaagta tggtcgttga tgaagtctta
    21721 cgtggaagaa tggaaaccag actgattaca aagattttgt catttgaaat taagtaatat
    21781 cctctctcct ttcgtggaag cgtgctaaac cattccacgc ttcccgaaca gggaggtttg
    21841 ttattccacc aaagcatatt gagctttcaa tgtgttttga taggctaacg agccattgtt
    21901 ctttgaaaac tgaataaaag taatcgaata cgtttcgata agaaaagagc caacggaact
    21961 aaccgccatg acctatctta taaagatagc gagcgattca tgttagtgat ccgagaagca
    22021 atctttagca ggattgcctg caacgacatt cttatcgtga taatgatact cccatacagt
    22081 caatagtccg agcgtgataa aaccgtcgca ggcaatgagt atggctacat gagaaccatg
    22141 caggggtgga actcccgtga gctttgctaa agctgttcga ttgctggtaa aacaactttt
    22201 atgaaatcca aataagtgat ttggaaagga ggattttatg aagcagactg acattcctat
    22261 ttgggaacgt tataccctaa ccattgaaga agcgtcaaaa tattttcgta ttggcgaaaa
    22321 caagctacga cgcttggcag aggaaaataa aaatgcaaat tggctgatta tgaatggcaa
    22381 tcgtattcag attaaacgaa aacaatttga aaaaattata gatacattgg acgcaatcta
    22441 gcgtcgccaa agggtcttgt atatgataaa atagtattaa gtcgtatcaa ggctctttcc
    22501 ataaaggaaa ggagcaaatg ccatgtcaga aaaaagacgt gacaataaag gtcgaatctt
    22561 aaagactgga gagagccaac gaaaagacgg aagatactta tacaaatata tagattcatt
    22621 tggagaaccg caatttgttt actcgtggaa acttgtggct acagaccgag taccagcagg
    22681 aaagcgtgat tgtatctcac ttagagagaa aatcgcagag ttacagaaag acattcatga
    22741 tggtattgat gttgtaggaa agaaaatgac actctgccag ctttacgcaa aacagaacgc
    22801 tcaaagacca aaggttagaa aaaacactga aactggacgc aaatatctta tggatatttt
    22861 gaagaaagac aagttaggtg taagaagtat tgacagtatt aagccatcag acgctaaaga
    22921 atgggctatt agaatgagtg aaaatggtta tgcttatcaa accatcaata actacaaacg
    22981 ttctttaaag gcttcattcc atattgctat acaagatgat tgtgttcgga agaatccatt
    23041 tgactttcaa ctgaaagcag ttcttgatga tgatactgtc cctaagaccg tactaacaga
    23101 agaacaggaa gaaaaactgt tagcctttgc aaaagctgat aaaacctaca gcaaaaatta
    23161 tgatgaaatt ctgatactct taaaaacagg tcttcgtatt tcagagtttg gtggtttgac
    23221 acttccagat ttagattttg agaatcgtct tgtcaatata gaccatcagc tattgagaga
    23281 tactgaaatt gggtactaca ttgaaacacc aaagaccaaa agtggcgaac gtcaagttcc
    23341 tatggttgaa gaagcctatc aagcatttaa gcgagtgtta gcgaatcgaa agaatgataa
    23401 gcgtgttgag attgatggat atagtgattt cctctttctt aatagaaaga actatccaaa
    23461 agtggcaagt gattacaacg gcatgatgaa aggtcttgtt aagaaataca ataagtataa
    23521 cgaggataaa ttgccacaca tcactccaca tagtttgcga catacattct gtaccaacta
    23581 tgcaaatgca ggaatgaatc caaaggcatt acagtacatt atgggacatg ctaatatagc
    23641 catgacgctg aactattacg cacatgcaac attcgattct gcaatggcag aaatgaaacg
    23701 cttgaataaa gagaagcaac aggagcgtct tgttgcttag tagtacaaat gaatttacta
    23761 cttatttacc acttctgaca gctaagacat gaggaaatat gcaaagaaac gtgaagtatc
    23821 ttcctacagt aaaaatactc gaaagcacat agaataaggc tttacgagca tttaagaaaa
    23881 tataaaaaga taattagaaa tttatacttt gtttgaacac aaaaatttat ttattttaac
    23941 tcatgataga gtggttaatg aagacgtaat ttcgattttt tcagaaatag attttttggt
    24001 tattgatgaa gtctataagc ttaaaa
//
LOCUS       DAUDYX010000030        23891 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.030, whole genome shotgun sequence.
ACCESSION   DAUDYX010000030 DAUDYX010000000
VERSION     DAUDYX010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23891
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.030"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            84..1502
                     /locus_tag="VHD02_001169"
     CDS             84..1502
                     /locus_tag="VHD02_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEQ5497783.1"
                     /translation="MDKKIQRFSCTTILVGKKASYDGSTMVARTEDSQNGDFTPKKMI
                     VVKPEDQPRHYRSVQSSFEMDLPDNPMTYTSVPDALGKDGIWAEAGVNEANVAMSATE
                     TITTNSRVLGADPLVASGIGEEDMVTLVLPYIRSAHEGVLRLGAILEDYGTYESNGVA
                     FSDEHDIWWLETIGGHHWIARRVPDDAYVTNPNQFGIDYFEFNNPEDYLCSADLKDFI
                     DTYHLDLTYNHEHFNPRYAFGSQRDKDRHYNTPRAWIMQKFLNPEIVQDPRSFALAWC
                     QKPYRKITVEDVKYVLSSHYQDTVYDPYGSEGTPVSKKVFRPIGINRTSQTAILHIRP
                     NKPQEIAAIQWMAYGSMPFNTMVPFFTQVKTIPDYFANTYENVSTDNFYWTNRLIAAL
                     ADPHYNHHETDLDNYLEETMAKGHAMLHAVEAQLLAGETVDLEEENQKMSDYVQGETQ
                     TLLNKILFDASNLMTNRFSLSD"
     gene            1654..3201
                     /locus_tag="VHD02_001170"
     CDS             1654..3201
                     /locus_tag="VHD02_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEQ5497784.1"
                     /translation="MKKKILLMMSLISVFFAWQLTQAKQVLAEGKVKVVTTFYPVYEF
                     TKGVIGNDGDVSMLMKAGTEPHDFEPSTKDIKKIQDADAFVYMDDNMETWVSDVKKSL
                     TSKKVTIVKGTGNMLLVAGVGHDHHHEEADKKHEHNKHSEEGHNHAFDPHVWLSPYRS
                     ITVVENIRDSLSKAYPEKAENFKANAATYIEKLKELDKDYTAALSDAKQKSFVTQHAA
                     FGYMALDYGLNQISINGVTPDAEPSAKRIATLSKYVKKYGIKYIYFEENASSKVAKTL
                     AKEAGVKAAVLSPLEGLTEKEMKAGQDYFTVMRKNLETLRLTTDVAGKEILPEKDTTK
                     TVYNGYFKDKEVKDRQLSDWSGSWQSVYPYLQDGTLDQVWDYKAKKSKGKMTAAEYKD
                     YYTTGYKTDVEQIKINGKKKTMTFVRNGEKKTFTYTYAGKEILTYPKGNRGVRFMFEA
                     KEPNAGEFKYVQFSDHAIAPEKAEHFHLYWGGDSQEKLHKELEHWPTYYGSDLSGREI
                     AQEINAH"
     gene            3349..4071
                     /locus_tag="VHD02_001171"
     CDS             3349..4071
                     /locus_tag="VHD02_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ5497785.1"
                     /translation="MPKEQPLYLQIVDDLEVKIKKSMTENDKLLSERELSDLYGVSRI
                     TIRLALKELELRDLIYKKQGKGTYVSGIKEPVTDLSSTYSFTEQMKKVGKTPKTEILS
                     FEQYQVTPYLSGLLEFDLDTEVFELERLRIADDMPLMFERSYIPAQPFQGLSIADLKR
                     KALYDIFAKDYQETIRLAEEEFYASIALDYEADLLGIKKGDPVLHIIRKTYNDKNILI
                     EFTFSIARADQFRYRVQHHPNG"
     gene            4091..5290
                     /locus_tag="VHD02_001172"
     CDS             4091..5290
                     /locus_tag="VHD02_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEQ5497786.1"
                     /translation="MFTKTQEALEALGAAITTKEIKQEPRLWQETMTFFEETRDSLDS
                     FLKRVCKSANGNNVHVIFTGAGTSEYIGNTICPYLKKVGNRQCYLFESVASTDLVAAP
                     DYYLAEEETVLLVSFARSGNSPESVAAVNLVNQLVPNSYHLTITCAKDGELAKKAQQD
                     ERSYLYLMPEDANDAGFAMTGSFTCMMLAALLIFDDANSIAQKEAYVTDMILAGQAVI
                     DQERRLQELADLGFERLVYLGSAGLAKLTQEAQLKMLELTAGQVATLYESSMGFRHGP
                     KSFINDRTLVIGFVNNDAYVRQYDLDMLEEIQADGIALKILALLQGGDINFSEDQFRL
                     DTRHLLPEAYLAFPMILVAQTLALLTAVTLGNSPDTPSATGTVNRVVKGVTIHPYPTS
                     SRQCNKA"
     gene            complement(5387..5647)
                     /locus_tag="VHD02_001173"
     CDS             complement(5387..5647)
                     /locus_tag="VHD02_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEQ5497787.1"
                     /translation="MRKDIHPDYRPVVFLDTTTGYQFLSGSTKASKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAK"
     gene            complement(5762..6703)
                     /locus_tag="VHD02_001174"
     CDS             complement(5762..6703)
                     /locus_tag="VHD02_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEQ5497788.1"
                     /translation="MITTFETILDKIKAHQTIIIHRHQNPDPDALGSQAGLKEIIAQN
                     FPDKKVLMTGFDEPSLTWISQMDQVTDKDYKEALVIITDTANRPRIDDERYTLGQCLI
                     KIDHHPNDDVYGDFYYVDTSASSASEIIADFAFSQNLTLSDKAAKLLYTGIVGDTGRF
                     LYASTTSKTLSIASQLRHFEFDFAAISRQMDSFPLKIAKLQSYVFEHLTIDESGAAYV
                     LVSQETLKHFDVTLAESSAIVCAPGKIDNVQAWAIFVELTDGNYRVRMRSKEKIINGI
                     AKRHGGGGHPLASGANSANLEENQAIFRELIAVCQEI"
     gene            7097..7546
                     /locus_tag="VHD02_001175"
     CDS             7097..7546
                     /locus_tag="VHD02_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEQ5497789.1"
                     /translation="MALAKIVYASMTGNTEEIADIVANKLQELGHDVDIDECTTVDAS
                     EFENADIAVVATYTYGDGDLPDEIVDFYEDLQDLDLEGKIYGVVGSGDTFYDYFCKSV
                     DDFSDQFALTGAVKGAEPVKVDLAAEDEDIDRLEAFAEQLSQAVNNL"
     gene            7721..8005
                     /locus_tag="VHD02_001176"
     CDS             7721..8005
                     /locus_tag="VHD02_001176"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEQ5497790.1"
                     /translation="MRLEEIRQEINGIDHHLVALLEKRMALVEQVTAYKLANHLPVLD
                     QARENQILDRVSYLVKDQAFEPAIHETFKTIMSLSRQYQTQRLTGGDIND"
     gene            7998..9260
                     /locus_tag="VHD02_001177"
     CDS             7998..9260
                     /locus_tag="VHD02_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEQ5497791.1"
                     /translation="MIKSIATLPKPSLKEALGLIGLALPIGATVGVVDVIFGKGLLFL
                     SEYRDHHLFLLLPFLALAGLVIVFLYDKLGKEVRQGMGLVFQVGHGQKDQIPPILIPL
                     ILFSTWVTHLFGASAGREGVAVQIGATISHYCQRFVTSQEAARHLLIMGMAAGFAGLF
                     QTPIAAVVFALEVLLVGTLRYCALLPSLVAACVASWTSHSLGLEKFTIVLEETLTITP
                     LTLVKLIGLGLIFGLVGNSFAYLLGWFKPYLSQKLPNPYFRIAFIGALLSICLMIGHV
                     GRYSGLGTHLIAAAFSGQTILTYDWLLKMIVTVISLSAGFQGGEVTPLFAIGASLGIV
                     LAPYLGLPVLLVAALGYTTVFGSATNTFWAPIFIGIEVFGPENALAYFVTSAAAYMVS
                     HRHSIYSYQKVVMINLKDMTVNQMDLKK"
     gene            9375..9722
                     /gene="rplS"
                     /locus_tag="VHD02_001178"
     CDS             9375..9722
                     /gene="rplS"
                     /locus_tag="VHD02_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEQ5497792.1"
                     /translation="MNPLIQSLTEGQLRSDIPNFRPGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVIRHGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            9969..10040
                     /locus_tag="VHD02_001179"
     tRNA            9969..10040
                     /locus_tag="VHD02_001179"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10002..10004,aa:Arg,seq:cct)
     gene            complement(10083..10340)
                     /locus_tag="VHD02_001180"
                     /pseudo
     CDS             complement(10083..10340)
                     /locus_tag="VHD02_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            10736..11305
                     /locus_tag="VHD02_001181"
     CDS             10736..11305
                     /locus_tag="VHD02_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEQ5497793.1"
                     /translation="MKGMNYQDYIWDLGGTLLDNYELSTQAFVQTLAFFSLPGDHDAV
                     YQKLKESTAIAVAMFAPNEPEFLHVYRLREADKLAQPIWCLGAKEILEKIATSGSRNF
                     LISHRDCQVNQLLEQAGLLIYFTEVVTASNGFARKPNPESLFYLKEKYDINSGLVIGD
                     RLIDKQAGQAAGFNTLLVDGRKNLLEIVT"
     gene            11306..13258
                     /gene="gyrB"
                     /locus_tag="VHD02_001182"
     CDS             11306..13258
                     /gene="gyrB"
                     /locus_tag="VHD02_001182"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEQ5497794.1"
                     /translation="MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEG
                     LHHLVWEIVDNSIDEALAGFASHIKVFIEADNSITVVDDGRGIPVDIQAKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQEFKRGAVV
                     ADLEVIGTTDVTGTTVHFTPDPEIFTETTQFDYSVLAKRIQELAFLNHGLKISITDKR
                     SGMEQEEHFHYEGGIGSYVEFLNDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQET
                     VMSFANNIHTHEGGTHEQGFRAALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFQRFLLENPQVARKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNDANQNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKARYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKEYIQPGIDQEDQ
                     LKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVTVDDAAEADKVF
                     DMLMGDRVEPRRDFIEENAVYSTLDI"
     gene            13650..15374
                     /gene="ezrA"
                     /locus_tag="VHD02_001183"
     CDS             13650..15374
                     /gene="ezrA"
                     /locus_tag="VHD02_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEQ5497795.1"
                     /translation="MSSGIILLIVAIVLLVIIAYLVGVIIRKRNDSLITSLEERKQAL
                     FALPVNDEIEEVKSLHLIGQSQTSFREWNQKWVDLTVNSFADIENHIFEAENLNDTFN
                     FIRAKHEINSVESQLNLVEEDIASIREALNILKEQEEKNSARVTHALDLYEKLQASIS
                     ENEDNFGSTMPEIDKQMKNIETEFSQFVALNSSGDPVEASEVLDRAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKNIEARFQEIRESIRANSSELV
                     TLDLDRAREENTHIQERIDSLYEVFEREIAAYKVAAKNSKMLPRYLAHVKRNNEQLKD
                     EIARLSRKYILSETESLTVKAFEKDIKEIEDSTLAVAEQFGLQEKPFSELQVTFERSI
                     KTLTNVESGQMDVFAAVKDIEKIESQARHNLDVYVTQLHMIKRYMEKRHLPGIPQDFL
                     SAFFTTSSQLEALMDELSRGRINIEAVSRLSEVATVAIANLEDLTYQVVQNATLTEQL
                     LQYSNRYRSFEAGVQSSFEHALRLFEVENDYQASFDEISYALETVEPGVTDRFVNSYE
                     KTREHIRF"
     gene            complement(15506..15964)
                     /locus_tag="VHD02_001184"
     CDS             complement(15506..15964)
                     /locus_tag="VHD02_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEQ5497796.1"
                     /translation="MTNLEDKLLKGAWGERRLNPEQQRYYLGTYAERVVLSALLDEAQ
                     TKTVKDYLKRELPSLQLVYQPLHLKISSKLAPQLQILYMKLAKENHLPVTIITETHMT
                     SPFAFILHTDHAINLKETRLEVILKQTKNDQLSKQTPEKTKSFWKRFLKK"
     gene            16197..17504
                     /gene="eno"
                     /locus_tag="VHD02_001185"
     CDS             16197..17504
                     /gene="eno"
                     /locus_tag="VHD02_001185"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEQ5497797.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYLGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMITLDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYTYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEGLRWGAEVFHALKKILKERGLVTAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQV
                     DYLEELVNKYPIITIEDGMDENDWDGWKVLTERLGKRVQLVGDDFFVTNTEYLARGIK
                     ENAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     gene            complement(17979..19010)
                     /locus_tag="VHD02_001186"
                     /pseudo
     CDS             complement(17979..19010)
                     /locus_tag="VHD02_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677767.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(19277..19837)
                     /locus_tag="VHD02_001187"
                     /pseudo
     CDS             complement(19277..19837)
                     /locus_tag="VHD02_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284650.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            19870..>23891
                     /locus_tag="VHD02_001188"
     CDS             19870..>23891
                     /locus_tag="VHD02_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEQ5497798.1"
                     /translation="MLQTVTVTKDQQNPISITLSEDQAKSLKNKEKLKVSIKQKQSKK
                     TSKDFFFEVGIDPKVEAKQKEKLLELDKVKKQIEDSINGDAWLPEKPEGEKPVQNTNK
                     ELQLQELNKKYQMAKEAIESATTLDDVETQFDKYTKVGDKDKYPDSLRNQYTQGDKDK
                     EIEKAKKSLGDLSDKVNGKIEEDKWLSAEVKKKQQQELEARKQKVNDSLKGSDSLKSL
                     RETVEKASSKNQKKPESFEDVYVPGNEETEKTKVRDILQKTYQKTEQNIETDPWLSPE
                     QKKAQKENAKTRLDAGLKAVETTESLDKLKEVESDFLDKEKNPDSIPNQHKAGTPEVA
                     RKTFLDNFEKEAKKEIESIKNDVTLTDAEKATAKAKVEAQLQEAKGKAKESKSFDDLK
                     NIQDKFNSELPHTPGKPLKDQQSDAIAELEKKQQEIEKAIEGDKTLPRDEKEKQIADS
                     KERLKSDTQKVKDAKNADAIKKVFEEGKVNIPQAHIPGDLNKDKEKLLAELKQKAADT
                     EKAIDSDKTLTAKEKEEQKAKTKEELQKATEAVGAIDNREELDKKVPELKKAIEDTHV
                     KGNLEGVKNKAIEDLKKVHDETVAKINGDDTLDKATKEAQVKEADKALAAGKDAITKA
                     DDADKVGAAVTEHTPKIKAAHKTGDLKKVQEEANQALDKAAEKERGEINNDITLTAKD
                     KEQQLKEVETALTKAKDNVKAAKTADAINDARDKGVATIDAVHKAGQDLGARKSGQVA
                     KLEEAAKATKDKISADPTLTSKEKEEQSKAVNAELKKAIEAVNAADTADKVDDALGKG
                     VTDIKNQHKTGDPVVARREAHGKQLDRVAQETKDAIEKDPTLTTEEKAKQVKDVDAAK
                     ERGMAKLTEAKNADELDKAYGEGVTDIKNQYKSGDPIEARRGLHNKSIDKVAQATKDA
                     ITADTTLTEAEKETQRGNVDKEATKAKEELAKAKDADALDKAYGDGVTSIKNQHKSGK
                     GLDVRKDEHKKALEAVAKRVTAEIEADPTLTPEVREQQKAEVQKELELATDKIAEAKD
                     ADEADKAYGDGVTAIENAHVIGKGIEARKDLAKKDLAEAVAKTKALIIEDKTLTDDQR
                     KEQLSGVDTEYAKGIENIDAAKDAAGVDKAYSDGVRDILAQYKEGQNLDDRRNAAKEF
                     LLKEADKVTKLINDDPTLTHDQKVDQINKVEQAKLDAIKSVDDAQTADAINDALGKGI
                     ENINNQYQHGDGVDVRKATAKGDLEKEAAKVKALIAKDPTLTQADKDKQTAAVDAAKN
                     TAIAAVDKATTAEGINQELGKGITAINKAYRPGEGVKARKEAAKADLEKEAAKVKALI
                     TNDPTLTKADKAKQTGAVAKA"
BASE COUNT         7774 a         4214 c         5000 g         6903 t
ORIGIN      
        1 ggaggtgctg ctggaattat gaaaattttt gtatccagtt caatggatat ttgatataat
       61 cgtcttaaaa aggaggtcgt gaaatggata aaaaaataca gcgtttttca tgtacaacta
      121 tactcgtcgg taaaaaggca tcttatgatg gctcaaccat ggttgctcga acagaagatt
      181 ctcaaaatgg tgatttcacg cctaaaaaaa tgattgtggt gaaaccagaa gatcaaccga
      241 gacattaccg ttcggtgcaa tcttcctttg aaatggactt accggacaat cccatgactt
      301 acacgtctgt tcccgatgcg cttggtaaag atggtatctg ggcagaagca ggcgtgaacg
      361 aggctaatgt ggccatgtca gcaactgaaa ccatcactac caattctcgt gttttagggg
      421 cagatcctct agtagcttct ggtattggtg aggaggacat ggtgacctta gtgctccctt
      481 atatccgttc tgcgcatgaa ggggttttgc gccttggtgc cattttagaa gactatggca
      541 cttacgagtc aaatggtgtg gctttttcag atgaacatga catttggtgg ctggaaacga
      601 ttggtggtca tcactggatt gcaagacggg tgccagatga tgcgtatgtc accaatccca
      661 atcaatttgg cattgattat tttgaattta acaatcctga agattacctt tgctcagctg
      721 atttaaagga ctttattgac acgtatcact tagatttaac ctataaccat gagcatttta
      781 atccccgtta tgcttttggt agccaacgtg acaaagaccg tcactataat acgccacgtg
      841 cttggatcat gcaaaaattc ttaaacccag aaattgtgca agacccgcgc agctttgccc
      901 ttgcttggtg tcaaaaacct taccgtaaaa taacagtaga agatgtcaaa tatgtattga
      961 gtagtcacta tcaggacacg gtttatgacc cttacggttc agaaggaact cctgttagta
     1021 agaaagtctt tcgccctatt ggcattaacc gtaccagcca aaccgctatc ttacacattc
     1081 gtcctaataa acctcaagaa attgcggcta tccagtggat ggcttatggg tcaatgccat
     1141 ttaataccat ggtgcctttt ttcacacagg tgaagactat tccagattat tttgccaaca
     1201 cttacgaaaa tgtgtctacc gacaatttct attggaccaa ccgtctgatt gcggctttgg
     1261 cagatcctca ctacaatcat catgaaactg atttggacaa ttacttggaa gaaaccatgg
     1321 caaaaggtca tgccatgtta catgctgtgg aagcacagtt attggctggt gaaacagtcg
     1381 atttggaaga ggaaaatcaa aaaatgagtg actatgtgca aggagaaacg cagacgctgt
     1441 tgaacaagat tctctttgac gctagcaatc taatgaccaa tcgtttcagt ttgagcgatt
     1501 aaaagtgaag ctaaggctga gcaactgctt agcctttttt acgataactt tttaggaaaa
     1561 gtaatcgcaa aaagacaaaa acttggaaaa aaaatcaaag tatgctatac ttaactggtc
     1621 aatttttaac cagttaataa caaaggagga atcatgaaaa agaaaattct tttaatgatg
     1681 agtttaatca gtgtcttttt tgcttggcaa cttactcagg caaaacaagt cttagcagag
     1741 ggtaaagtga aggtggtgac aactttctat cctgtttatg aatttacaaa aggggttatt
     1801 ggtaatgatg gcgatgtttc catgcttatg aaagcaggaa cggaacctca tgattttgag
     1861 ccttctacaa aagacattaa aaaaatccaa gatgcagatg catttgttta tatggatgac
     1921 aatatggaaa cttgggtttc tgatgtgaaa aaatcattga catctaaaaa agtgaccatc
     1981 gtcaagggaa ctggtaacat gctcttggta gcaggagttg gacatgacca tcaccatgag
     2041 gaagctgaca aaaagcatga gcataataaa catagcgaag aaggacacaa ccatgctttt
     2101 gacccacacg tgtggttgtc accataccgt agcattacgg tcgttgaaaa tattcgcgac
     2161 agtctttcaa aagcttaccc agaaaaagca gagaacttca aagccaatgc cgctacttat
     2221 attgaaaaat taaaagagct tgacaaagac tatacggcag cactttcaga tgctaagcaa
     2281 aagagctttg tgacacaaca cgcagctttt ggttatatgg cacttgacta tggcttgaac
     2341 caaatttcta ttaatggtgt cacaccagat gcagaaccat cagcaaaacg tattgctact
     2401 ttgtcaaaat acgttaaaaa atatggtatc aaatacattt attttgagga aaatgcgtca
     2461 agtaaagtcg caaaaaccct agctaaagaa gcaggagtta aagcggctgt gcttagtccg
     2521 cttgaaggtt tgactgaaaa agagatgaaa gctggccaag attactttac ggtcatgcgt
     2581 aaaaaccttg aaaccttacg cttaaccact gatgtggctg gtaaagaaat tcttccagaa
     2641 aaagacacga ctaagacagt ttacaatggt tatttcaaag acaaagaagt caaagatcgt
     2701 caattatctg actggtcagg tagctggcaa tctgtttacc cctatctaca agatggtact
     2761 ttagaccaag tttgggacta taaggctaaa aaatctaaag gtaaaatgac agcagccgag
     2821 tacaaagatt actacactac tggttataaa actgacgtgg aacaaatcaa aatcaatggt
     2881 aagaaaaaga ccatgacctt tgttcgtaat ggtgaaaaga aaaccttcac ttacacatac
     2941 gccggcaaag aaatcttgac ctatccaaaa ggaaatcgcg gagttcgttt catgtttgaa
     3001 gctaaagaac caaatgctgg cgaattcaaa tacgttcaat tcagtgacca tgccattgct
     3061 cctgaaaaag cagagcattt ccacctgtac tggggtggtg acagccaaga aaaattacat
     3121 aaagagttag aacattggcc aacttactac ggttcagact tatctggtcg tgaaattgcc
     3181 caagaaatca atgctcatta atcttaactc actaagcaaa tcttctttta aggaggtttg
     3241 ctttttcttt gtggagtaaa aaaatttaat aattctcaac attaatttga cggacactta
     3301 tgattataat atactttaag tggttataac aagttagagg tgaataagat gccaaaagaa
     3361 caaccactct atctacagat tgttgatgac ttggaagtga aaatcaaaaa gagcatgaca
     3421 gaaaacgata agttgttgtc agaacgagaa ttaagtgacc tttacggtgt tagtcgtatc
     3481 acgattagac tggctcttaa ggaattggaa ttacgagact taatttataa aaagcagggc
     3541 aaggggactt atgtttcagg aatcaaagaa cctgtcactg atttatcaag tacttacagc
     3601 tttacagagc aaatgaaaaa agtggggaaa actcctaaaa cagagattct ttcttttgaa
     3661 cagtatcagg taacacctta cttgtctggt ctcttagagt ttgatctcga tacagaagtt
     3721 tttgaattag agcgcctaag gattgcagat gatatgccct tgatgtttga acgatcctat
     3781 attcctgcgc agccttttca ggggttaagc attgcggact tgaagcgtaa agctttgtac
     3841 gatatctttg caaaggacta tcaagagaca atccgtcttg cagaagaaga gttttatgcc
     3901 agcattgcct tggactatga agcagacctt ttgggaatca aaaaaggaga tcctgtctta
     3961 catattattc gtaaaactta taacgataaa aatattttga tcgaatttac tttcagtatt
     4021 gcaagagcag accagtttag gtatagggta caacatcacc ccaatggtta aagaaatggg
     4081 aggttaatac atgtttacaa aaacgcagga agctttggag gctcttggtg cagctatcac
     4141 taccaaggaa attaaacaag aaccccgttt atggcaagaa accatgacgt tttttgaaga
     4201 aacaagagat tctctggatt cttttttaaa gagagtttgt aaaagcgcta atggaaataa
     4261 cgttcatgtc atctttacag gtgcgggtac atctgagtac atcggtaata ccatttgccc
     4321 ttacttgaag aaggtgggga atcggcagtg ctatctcttt gagagtgttg cttcaaccga
     4381 tttggttgcg gcgccagatt attatcttgc tgaggaagag acggtactat tagtctcctt
     4441 tgctaggagt ggtaatagtc cagaaagtgt agcggctgtt aacctggtca atcaactagt
     4501 gccaaatagt tatcatttaa ccattacgtg tgccaaagat ggagaactgg cgaaaaaagc
     4561 acagcaagat gagcgttctt acctttattt gatgcctgaa gacgctaatg atgctggttt
     4621 tgccatgact ggtagtttta cctgtatgat gctagcggct cttttgattt ttgatgatgc
     4681 taacagtata gctcagaaag aagcttatgt gactgatatg attcttgctg ggcaagctgt
     4741 tattgaccaa gaaagacggc tacaagagct agctgattta ggatttgaac gtttagttta
     4801 tctaggttct gctggtttag ccaagttaac ccaagaagct caattaaaaa tgttagaatt
     4861 gacagctgga caagttgcga ccctctacga gtcctctatg ggctttaggc atggtccgaa
     4921 atcttttatt aatgaccgta ccttagtcat tggatttgtc aataatgatg cttacgtcag
     4981 acagtacgat ttggacatgt tagaggaaat ccaagcagat ggcattgccc ttaaaatctt
     5041 ggctctattg caaggagggg atatcaactt ttcagaggac caatttcgac tagacacccg
     5101 tcacttattg ccagaagctt acttagcttt tcccatgatt ttagtcgcgc agaccttggc
     5161 cctcttaacg gccgttacat taggaaattc ccctgacacc ccatctgcga caggaacggt
     5221 taatcgtgtg gtcaaaggag taacgattca cccttacccg actagtagtc gtcaatgcaa
     5281 caaagcttaa agcttttgtg tagaagccgt aaaacgaaat gattttgtgc tagtaggcca
     5341 aaagaaaaac cctcctgata caggaaggtt ttttgtattg tcttgcttat tttgctgcgt
     5401 ttgcgtcttt gagaccgtat tttttgttga agcggtccac acgtccatct gcttgagtga
     5461 acttttgacg tcctgtgtag aatgggtgtg aatctgatga aatttccaca cggataagtg
     5521 ggtaagtttc accttcaaac tcaacagttt ctttagaagc ttttgttgag ccgctaagga
     5581 actggtaacc tgtagttgta tcaaggaaaa caactggacg ataatctgga tgaatgtctt
     5641 ttctcatctt aaaaaattcc tttctgccat gacacttgat gcatcatagt tattaactat
     5701 tctagtgtaa caaaacttga atgacttgac aaggcttttc tttccttatt ttttaaaaat
     5761 cctaaatctc ttggcaaaca gcgatgagtt ctcggaaaat agcttgattt tcttctaaat
     5821 tagctgagtt ggctccgcta gcaaggggat gcccccctcc accgtgacgc ttagcaatgc
     5881 cattaataat cttttcttta ctgcgcatac gcacacggta gttgccgtca gttaactcaa
     5941 caaaaatagc ccaagcttga acgttatcaa ttttaccagg agcacagaca atggcagagc
     6001 tttctgctag ggtcacgtca aaatgtttta aggtttcttg gctgacaagg acataagcag
     6061 ccccactctc atcaattgtt aaatgctcaa agacgtagct ttgcagcttt gctattttca
     6121 aaggaaacga atccatttgc cttgaaatcg cagcaaagtc gaactcgaaa tgtctgagtt
     6181 ggctagcaat ggaaagggtt ttactagtgg ttgaggcata aagaaatcgc cctgtatcac
     6241 caacgatacc agtgtataag agcttagcag ccttgtcaga gagagtaaga ttctgactaa
     6301 aggcaaagtc tgcaatgatt tcacttgcgc tagaagcgct tgtgtccaca taatagaagt
     6361 caccatacac atcatcgttg ggatggtgat caatcttaat taagcactgc cccagtgtgt
     6421 agcgctcatc atcaatcctt ggtcgattcg ctgtatctgt aatgatgacc aaagcctctt
     6481 tatagtcttt atcagtcacc tgatccattt ggctaatcca agttaaacta ggctcatcaa
     6541 aaccagtcat caaaaccttt ttatctggga aattttgtgc aataatttct ttcaagccgg
     6601 cctgactacc aagagcatca gggtcaggat tttgatggcg atggataata atagtttggt
     6661 gagcttttat tttatctaaa attgtttcaa aagttgttat catgctttaa tctttctttt
     6721 tcaaaaagag gaaaataacc gtttaacata gtcttcaaaa aacggtttgt gtcataagga
     6781 ggtgtgcgtt atgttaagtg gctaatgcta gaatttttta agattgtcaa agctcataaa
     6841 ttgacatttt atctgaaaat catatcctat tttattatag cataaaagta agctctagat
     6901 aatggtaaat taatctagga agaaaagagg ttagttatca gttatgatac tatcaaagct
     6961 agttttgcaa atgcaagtga aaaaatagtt tttaagtgtc ttaagtaatc atatcaactt
     7021 tctctttaaa caattagtgc ttttcctaaa aaagtgtgct aaaataaaca tagtttagta
     7081 attttgggag gaatttatgg cattagcaaa aatcgtttat gccagcatga cgggaaatac
     7141 tgaagaaatc gctgatatcg ttgcaaataa attgcaagaa ttgggccatg atgttgacat
     7201 tgatgagtgc acaacagttg acgcttcaga gtttgagaat gctgatatcg ctgtcgttgc
     7261 gacttatact tatggtgacg gtgacttacc agatgaaatc gttgattttt acgaagattt
     7321 acaagattta gatttagaag gtaaaatcta tggcgttgtc ggctctggtg acacttttta
     7381 tgactatttt tgcaaatctg ttgatgactt ttcagatcaa tttgcgctta ctggtgccgt
     7441 caaaggtgca gagccagtca aagtagattt ggcagcagaa gatgaagaca tcgatcgttt
     7501 agaggccttt gcagagcaac tctcacaggc tgttaataat ctataaaact atttagtaaa
     7561 aatgtcaggg acttccttgg catttttaga tttttaacct aaaataaagg gaatgtcact
     7621 tcagggtagg aattgaaaaa acaatcagct tcaccttgct tctgatcctc ttattaacta
     7681 taataaaaag ataatctaat acatctagaa aaggaaagtt atgcgtttag aagagatacg
     7741 acaagaaatc aatggtattg atcaccactt ggttgcctta ttagaaaagc ggatggcact
     7801 tgtagagcaa gtaacagctt ataaactagc caatcacttg cctgtcttag atcaggcgag
     7861 ggaaaatcag atcctagata gggtttctta tctcgttaaa gaccaagcat ttgagccagc
     7921 tatccatgag acttttaaaa ccattatgtc tctttcacgg cagtatcaaa cgcaacgctt
     7981 aacaggtggt gacattaatg attaaatcaa ttgcaacact gcctaaaccc tccttaaaag
     8041 aagcgttagg actaataggc ttggcgcttc ccataggggc cacggttgga gtggttgatg
     8101 tgatttttgg taaagggctt ttgtttcttt ccgaatatcg cgatcaccac ctgttcttgc
     8161 ttcttccttt tcttgcccta gcaggtcttg ttatcgtttt tctttacgat aagttaggca
     8221 aagaggttcg tcaagggatg ggattggttt ttcaggtagg gcatggtcaa aaagatcaga
     8281 ttcctcctat tctcattcct cttattttgt tctcgacttg ggtaacccac ttatttggag
     8341 cgagtgcggg tcgagaaggg gtagccgttc aaataggagc tacgatctct cattattgtc
     8401 agagatttgt gactagtcaa gaagccgcac gacacctttt gattatgggg atggcagccg
     8461 gttttgcagg actttttcaa acgccgattg ctgctgtcgt atttgccctt gaggttttgt
     8521 tggtaggaac gcttcgctac tgcgctttat tgccttcctt agttgctgct tgcgtggctt
     8581 cttggacctc tcattctttg ggattagaga aatttacgat tgttcttgaa gaaacattga
     8641 ccataactcc cttaacattg gttaaattaa ttgggttagg ccttattttt ggtctggtgg
     8701 gaaatagttt tgcctattta cttggctggt ttaaacctta cctcagtcaa aagctaccaa
     8761 atccttattt ccggattgct tttatcggtg ctttgttatc tatctgtctg atgattggtc
     8821 acgtcggaag atatagtggt ctaggaactc atttgattgc ggcagctttt tctggccaaa
     8881 ctatcctaac ttacgactgg cttttaaaaa tgatcgtgac ggtgattagc ttatctgcag
     8941 gctttcaagg tggtgaagtg acgcctctat ttgctattgg agcttcttta ggcatcgttt
     9001 tggcccctta cttaggtctt cctgtactgt tggttgctgc acttggttac accacggtat
     9061 ttggaagtgc aacaaatacc ttttgggccc ctatctttat tgggatagag gtctttggtc
     9121 cagagaatgc cctagcatat tttgtcacct cggctgcagc ctatatggtt agtcacagac
     9181 attccattta cagttatcaa aaagtagtca tgatcaattt aaaagacatg acggtaaatc
     9241 agatggattt aaaaaaatag ctttacaata aagaagcttt ttgttagaat aaatctcgtg
     9301 tgtaatggac acacgaaaat tacggctaat ccgctaagac aagtacttaa gattagtaag
     9361 ataggagaat aaaaatgaat ccattaatcc aaagtttgac agaaggtcaa cttcgctctg
     9421 atatccctaa cttccgtcct ggtgacactg ttcgcgttca cgcaaaagtt gtcgaaggaa
     9481 ctcgcgaacg tatccagatc tttgaaggtg ttgttatttc acgtaaaggt caagggatct
     9541 cagaaatgta cactgttcgt aaaatctcag gtggtatcgg ggtagagcgt acattcccaa
     9601 tccacacacc acgtgttgac aaaatcgaag ttatccgcca cggtaaagtc cgtcgtgcga
     9661 aactttacta cttgcgtgca ttacaaggta aagctgctcg tatcaaagaa attcgtcgtt
     9721 agtttgaacc gaaacgtaac gtaatagaat aaaaagacta gagtctctag tctttttatg
     9781 tcgtcgtgac aatgtgacaa ttggtgatta acgctatgct cgtaggacac tttgaaagtt
     9841 ccttagcagt tataattagt atattgtaat aacaaaatga agcaaaataa actttcagtt
     9901 atctgatatt tcatcttgca ttaccttatt ttctaggtta aaatagaaga caaggtgaat
     9961 ataagatagg ccccttagtt caatggatat aacaactccc tcctaaggag tagttgctgg
    10021 ttcgattccg gcaggggtca ttattgtcac tgaaaaagcc tttacatcaa aggctttttg
    10081 ttttattccg gttttaaaag gggcataaaa ggggcagttt gtttatttat aatttctttc
    10141 atgtttacag ttgtgtgact gtaaatagat aatgttgttt ttggatcgct gtgaccaact
    10201 ctatccatta tcgcatttag cggtatccct ttttctgcta aaaatgatat atgcgagtgt
    10261 ctaaataagt gcgtgtgata atctccgtaa attttcaatc gcttattgat gtgtgcattt
    10321 aaaattggta aaccttttga atatggaaag ctgttagcac tctgcttgtg attaatctat
    10381 cgtatgttaa aagtgacatt taaattaaat cattttttat acatatattt caacaggatt
    10441 tatcatattg gaaaatagcg gtgcttctat tggcatttta ctattgacag gaatttccct
    10501 gtcttttgtc tcctaaagaa gatcagcttt gatttctaac ttgattagag tgggcttggt
    10561 actgtttatg aattatcagt atatactttc tggagatcaa aaaggaaagg aacagctaaa
    10621 gaggtgtatg tgttaccatt agaggaatag aacatcgcaa gactcgttta ttcagtcatt
    10681 acgatacctg ttttatttgg gaaataacta taatattaag tgaatttgtg atataatgaa
    10741 aggtatgaat tatcaagatt atatatggga tttgggaggt accttactcg ataattacga
    10801 gctttcaacc caagcttttg tccaaacgct agcttttttc agccttccag gagatcacga
    10861 tgctgtttac caaaaattaa aagaatcaac cgctatcgct gtggcaatgt ttgctcctaa
    10921 tgagccagaa tttttgcatg tgtataggct aagagaagca gacaaattgg cacaacccat
    10981 ttggtgtttg ggagctaaag aaattttgga aaagatagcg acttcaggtt cgcgaaattt
    11041 tttaatttct catcgagatt gtcaggtgaa ccaactttta gagcaagctg ggttgctaat
    11101 ctattttaca gaagtcgtga cagcttcaaa tggctttgct cggaaaccaa atcctgaaag
    11161 tttgttttat ttaaaagaaa agtatgatat taatagtgga ttagtaattg gagatcggct
    11221 aattgataaa caagcaggac aagcagcggg ctttaatacc ttgcttgttg acggtagaaa
    11281 aaacctattg gagatagtaa cgtagatgat tgaagaaaat aaacattttg aaaaaaaaat
    11341 gcaagaatac gatgccagtc aaattcaggt tctagaaggg ctggaggctg tccgcatgcg
    11401 tccagggatg tatattggct cgacagctaa agagggtttg catcatttag tctgggaaat
    11461 tgttgacaac tcaattgacg aagcattagc aggttttgcc tctcatatta aagtctttat
    11521 tgaagcagat aattccatta cagtagtaga tgatggccgt ggaattccag ttgacatcca
    11581 agccaagaca ggacgtcccg ccgtcgaaac agtttttaca gtcttacacg caggtggtaa
    11641 atttggcgga ggcggctata aggtttctgg aggattacat ggtgtagggt catctgttgt
    11701 taatgcttta tcaacacaat tagatgtacg tgtttataaa aacggccaaa ttcattacca
    11761 agaatttaaa cgcggggctg ttgtagcaga tcttgaggtc attggaacca ctgatgtgac
    11821 tggaacaacg gtacacttta cacccgatcc agaaattttt accgaaacga ctcagtttga
    11881 ttacagtgtt ttagcaaaac gtattcaaga gttagccttt ttaaaccatg gtttaaaaat
    11941 ttccattaca gataagcgtt caggtatgga acaagaagaa catttccatt atgaaggtgg
    12001 aattggttct tatgttgaat ttttaaatga taaaaaagat gttatctttg aaacgcccat
    12061 ctatacagat ggtgaattag aaggtattgc agttgaagta gccatgcaat atacgactag
    12121 ctatcaagaa acagtcatga gttttgctaa taatattcat actcatgaag gtggaacgca
    12181 tgaacaaggc tttagagcgg ctcttactcg ggtcatcaat gactacgcta agaaaaataa
    12241 aattcttaaa gaaaatgagg acaatttgac aggagaagat gttcgtgaag gtttgacggc
    12301 ggtaatttct gttaagcatc caaatcctca atttgaaggt caaaccaaaa caaaattggg
    12361 caactcagaa gtggttaaga tcactaatcg tctctttagt gaggcctttc aacgttttct
    12421 tttggaaaac ccacaagttg ctcgtaagat tgtggaaaaa gggattttgg cttctaaagc
    12481 tagaattgca gctaagcgag cccgcgaagt cacccgcaaa aaatcaggct tagaaatttc
    12541 aaacttacct ggaaaattag cagactgttc gtcaaatgat gctaaccaaa acgaactttt
    12601 catcgtcgaa ggagattcag cgggtgggtc ggccaaatca ggtcgtaacc gagagtttca
    12661 agctatcttg cctattcgcg gtaaaatttt gaacgtggaa aaagcaacta tggataagat
    12721 tcttgccaac gaagaaatta gaagtctctt taccgctatg ggtacaggtt ttggtgcaga
    12781 ctttgacgtg tcaaaagctc gctaccaaaa gctggttatc atgaccgatg ccgatgtgga
    12841 tggcgctcat attagaacct tgcttttaac tttgatttac cgctttatga ggcctgttct
    12901 agaagctggc tatgtttaca tcgcccagcc acctatttat ggtgttaagg tcggtagtga
    12961 gattaaagag tatattcagc caggtattga tcaagaagac caattaaaaa cagctcttga
    13021 aaaatatagt attggtcgtt caaaaccaac tgttcaacgt tataaaggtc ttggggaaat
    13081 ggatgaccat caactttggg aaactactat ggatcctgaa aatcgtttga tggcgcgtgt
    13141 gacagttgat gatgccgcag aagcagataa agtatttgat atgttaatgg gagatcgtgt
    13201 tgaaccaaga cgtgatttca ttgaggaaaa tgcggtttat agtacactgg atatttagtc
    13261 acctttttag tttcacaagg aatctgctac caaagaggac gaattgacta aaaatcatga
    13321 tgcatgatgc caacagcttt gttagttagt acgtcttcat ctttgttagt tagtacgtct
    13381 tcatcttgag ggacagcgtt aatgcatagg tattgagacc gccccttaat acctatgtta
    13441 gtccttatta agaaagcctt gtgagtcgct ggtacgagat tgttgaatta gtcaaatata
    13501 ttttgaaatg cattttaaag tatgttataa tcgtaatgtt atagtgtatt ttaaataaaa
    13561 ttttaataaa aggcgattga ggaatttcag tttttagtta ttactggaac aatgttgata
    13621 aatcgtgtct aaataataag gagattcaga tgtcaagcgg aattatcttg ctgattgtgg
    13681 ctatagtcct actagtgatt atcgcctatc tagtgggtgt catcatacga aagcgtaatg
    13741 attccctaat taccagtcta gaagaacgaa aacaggcctt gtttgctttg cctgttaatg
    13801 atgaaattga agaagtaaag tcacttcatt tgataggaca gagccaaacc tcttttcggg
    13861 aatggaatca gaaatgggta gacttaactg taaattcgtt tgcagatatt gaaaatcata
    13921 tttttgaagc tgaaaatttg aatgatacgt ttaattttat tcgcgccaag catgagatta
    13981 atagcgttga aagtcagctt aatcttgttg aagaagatat cgcttctatt cgtgaggcac
    14041 ttaatatctt aaaagagcaa gaagaaaaaa atagtgctcg tgtcacccat gctcttgatt
    14101 tatatgaaaa gctccaagct tctatttctg agaatgaaga taattttggt tcaactatgc
    14161 ctgaaattga caaacagatg aaaaacattg aaactgaatt ttcacagttt gtcgcactca
    14221 actcatcagg tgatcctgta gaagcttctg aagttctgga tagagcagaa gagcatacta
    14281 ttgctctagg gcagattaca gagcagattc cagctattgt cgccaagttg gaagatgatt
    14341 ttccagatca gttagatgat ttggaaacag gatatcgccg tttacttgaa gaaaactatc
    14401 attttccaga aaaaaacatt gaggcacgtt ttcaggagat tcgtgagtct attcgtgcca
    14461 attcatcaga gttagttaca cttgacttgg atcgtgctag agaagaaaac acgcatattc
    14521 aagaacgcat tgattctctt tatgaagtct ttgagcgcga aattgccgct tataaggtag
    14581 ctgctaaaaa tagtaagatg ctacctcgtt acctagcaca tgtgaaacgt aataatgaac
    14641 aactcaaaga tgaaattgca cgtttatcac gtaaatatat tctaagtgaa acggaaagtc
    14701 tgactgttaa ggcatttgag aaggatatta aagagatcga agacagtaca cttgctgttg
    14761 cagaacaatt tggtttacaa gaaaaaccat tttcagaact gcaagtgact tttgaacgca
    14821 gtataaagac gcttactaat gtcgagtcag gtcaaatgga tgtttttgca gcagttaaag
    14881 atattgaaaa aattgaatca caggcacgcc ataaccttga tgtttatgtt actcagcttc
    14941 atatgattaa acgttatatg gaaaaacgtc acttgcctgg aattccacaa gactttctta
    15001 gtgctttctt tacaacgagt tcacagttgg aagctttgat ggatgagctt agtagaggac
    15061 ggatcaatat tgaggcagtg tcacgtttgt cggaagttgc gacggttgct attgctaatc
    15121 tagaagattt aacctatcaa gtggttcaaa atgctacctt gactgaacaa ttactacaat
    15181 attctaatcg ctatcgttct tttgaagcag gagttcaaag tagttttgaa catgctttga
    15241 gacttttcga agtagaaaat gattatcagg cttcttttga tgaaatttct tatgctcttg
    15301 aaacagttga acctggggta acagatcggt ttgttaactc ttatgaaaaa acacgagagc
    15361 acattcgttt ttgatacttg aaagtcgtga tattctcacg actttttatt agctttttgg
    15421 accaatacgt gtttttgact agaatcttct ttttaatgat caaaagaaga agtagtaaca
    15481 gtgttttttg ctattgagag gatagttatt ttttcaaaaa tcttttccaa aaggatttgg
    15541 ttttttcagg agtttgtttg ctaagttgat cgttttttgt ctgctttaag atgacttcta
    15601 aacgcgtttc ctttagattt attgcgtggt cggtatgaag gataaacgca aaaggtgaag
    15661 tcatatgcgt ttctgtaata atagtaacag gtaaatggtt ttcttttgct aatttcatat
    15721 agagtatttg aagttgaggt gccagcttgg aagagatttt taagtgtaaa ggttgataga
    15781 ctaactgaag gctcggtaat tctcttttca aatagtcttt tactgtttta gtttgagctt
    15841 cgtctaataa agcacttaaa acgactcgtt cagcatacgt tcctagatag tagcgttgct
    15901 gctcaggatt taatcttctt tctccccagg ctccttttaa tagcttatct tctaaattag
    15961 tcatagatac cctcgcttct ttataataat aatagtttct ttattatatc gatttctaca
    16021 ataaaatacc aatttgaaaa ttttcggtaa atgtgttaaa aaacaagaaa gaaagtgatt
    16081 tttcttgcaa aaaacaaagg aagcttttca tgagattgct ttttaagttg attaaaggcc
    16141 tagtttgtgg tattatgaac atgtaaaata ttaaaactca taaggagagt attacaatgt
    16201 caattattac tgatgtatac gctcgcgaag tccttgactc acgcggtaac ccaacacttg
    16261 aagtagaagt ttatacagaa tcaggtgcat tcggacgtgg tatggttcct tcaggagctt
    16321 caactggtga acatgaagcc gttgaacttc gtgatggtga caaatctcgt taccttggtc
    16381 taggtactca aaaagcagtt gataacgtta ataacattat cgctgaggca atcatcggtt
    16441 acgatgttcg cgatcaacaa gctatcgacc gtgcaatgat cactcttgac ggtactccta
    16501 acaaaggcaa acttggtgct aatgctattc ttggtgtttc tatcgctgtt gctcgtgcag
    16561 ctgctgacta tcttgaagtg ccactttaca cttaccttgg cggattcaac actaaagttc
    16621 ttccaactcc tatgatgaac atcatcaacg gtggatcaca ctcagatgcc ccaattgcat
    16681 tccaagagtt catgatcatg ccagttggtg cacctacttt caaagaaggt cttcgttggg
    16741 gtgctgaagt tttccacgct cttaagaaaa ttcttaaaga acgcggactt gttacagctg
    16801 taggtgacga aggtggattt gcacctaaat ttgaaggaac tgaagacggt gtagaaacta
    16861 tccttaaagc tattgaagca gctggttacg aagctggtga aaacggcatt atgatcggtt
    16921 ttgactgtgc atcatcagaa ttctacgaca aagaacgtaa agtttacgac tacactaaat
    16981 ttgaaggtga aggcgctgct gttcgtacat ctgcagaaca agttgattac cttgaagagt
    17041 tggttaacaa atacccaatt attactatcg aagatggtat ggacgaaaac gactgggatg
    17101 gttggaaagt tcttactgaa cgcctaggca aacgtgttca attggttggt gacgacttct
    17161 tcgttacaaa cactgaatac cttgctcgtg gtatcaaaga aaatgcagct aactcaatcc
    17221 ttatcaaagt taaccaaatc ggtactttga ctgaaacgtt tgaagctatc gaaatggcta
    17281 aagaagctgg atatactgcc gttgtatcac accgttcagg tgaaactgaa gattcaacaa
    17341 tcgctgacat cgcagttgca acaaacgctg gccaaatcaa aacaggttca ttgtcacgta
    17401 cagaccgtat tgctaaatac aaccaactac ttcgtatcga agatcaactt ggtgaagttg
    17461 ctcaatacaa aggtatcaaa tcattctata acttaaaaaa atagacggta agacagaaat
    17521 cggtcattcg tagaatgcga tttcgtcgtc gcacctccgt caggctgact aggttggtca
    17581 cgacaaaagg agtggacatc cagtcagcta cgacgcaaaa gttaaagaat agaaagcttg
    17641 ggaaaatccc aagctttttg ctttctaaat agtaaaaatt tactgtgggc taatttatcc
    17701 tgagctaatg tattagcgag gtaaagttac tgaagaagac ttggatttgt actgcctcta
    17761 aaaagttgga cactatattt taaatacagg actaagtcct tttataccaa ttgtcaagca
    17821 catcaaggtt ttaactgaaa aaatgtttta gacaatagcc taggtttagc tgttgtctta
    17881 cttgttgtgc tctgattagc ataaaaaagt gtacaagaaa aaaaccttgc ctagtatttt
    17941 taagatcaag gtgttacaat gatagagtaa caacgacttt aatgattttg ggtcgaagcg
    18001 taattgtaaa gtttgttatg cgttatgagg taaagcattg tcctgacaag gcaatgtcta
    18061 gaggcaatcg tatgtggctt ggttgaagct gtttacgatt gcctttttcg tttctcatag
    18121 aagtctgcga tatgacaagg attggtatga ctagcagaag caatgtcatg gatacacttg
    18181 aaaagaatct ttggagcata aggattccca cgttcggtaa tgtgctcttg agctagacag
    18241 ttgccagact cataatgtct aaagtcaatc ccaatgaaag cattaatttg attagcagat
    18301 tgaaaacgac gaatagcacc gagttcgtca ataatacttg ttgcagttgt ttctgcgata
    18361 cttggaatag agcgtagaat cttgtcttct ggtaaaggtt gagccaacgc caccatctta
    18421 tcaaaaatag cttgacgacg ttcagaaagt ctaagtaatt ctttggcata atagcgaacc
    18481 tcttctatca tcggagaggt tttcgtaaca gtaaaataag actgcttggc taatgagaca
    18541 agtttttcca ctaagtagat cccacgcttg tcagagatac gtttagaggt agattgacgg
    18601 atgatagctg acaatgcttt gtcagctagc tccagtacaa agctattaca tggaaaagcc
    18661 gttaccacat tccagtatta ctcaccagtt ggtgcaaata agatgtgttc cagttcagga
    18721 aaagtgactt gtaagatctt gtgaaggcga ttcttaactc tcgaacaata ttatcctcag
    18781 tctcaagttt tgatagaagc gactcaagtc acgtagcagc tgataaactt cttcctggac
    18841 gtaagttggt tttctaacta gaataaattg agactgagcc aatttttcag tgtctaactt
    18901 atccgcttta cgcacccgta gactatctag ttttctaaag gattgagttg tgtataagcg
    18961 tagccatttt tctcaaggaa ggactgaagc cgacgagaat agactcctgt tgtgatggtc
    19021 tcccttttgt cttttaaaca atagtatagc cgtttttcaa aatgttatta atgatttttt
    19081 cctttattct agaagaataa gtctgatact gtttcttctt aataatctca atgccctaac
    19141 aatccatcag tcgaacggta tttctcaaat tactgcagtt gactcttgcg ttcagttccc
    19201 tcaccttgtg gatgccgttt agaacaatat tagcttgctg agccttttgg taatgtgaac
    19261 aaagcatctt tatcactcaa ggagaagaca ggttcttgtg gtaagtgtcc aacaaggtca
    19321 cgataaactc atcgtaccgt ttttggcaga agtctagaaa tgccttatga atgtaagcct
    19381 cagaaacgtc tctaaaacta aacaagaaat acaagaacaa atgcccttga ttctggtaca
    19441 aatgctgtat caggttcagg tattgcctat ctgcgataat gacactctgc ttttccttaa
    19501 caatatcttt gaaaggaatg atttcgacat tgacaggcat aaccgttgca atagctggcg
    19561 ggaagtaaat cgagagtgca ttggtcatca aatccagcgc agcagtgtta ttggtcaaaa
    19621 gatagacagg tatcggtggc aaatatgttt ttaggagttc ctctaactgt atcgtgaaga
    19681 gcagtaaatg ataggacggc agtttcccca cgataccttc tgcgctcatc cattcctcaa
    19741 cgatggtttt tagagtattg tagaagctct tgtttcgttt ttgctgtctt ctgaggtaat
    19801 ggaacaagtg tatatcaggt aacaaacttc gaaggtgatg agataagcct tttcaactct
    19861 aggggagagt tgttacagac agttacagtt acaaaagatc aacaaaatcc tatatcaata
    19921 actttatctg aagatcaagc caaatcttta aaaaataaag aaaagttaaa agtaagcatt
    19981 aaacaaaagc aatcaaaaaa gacttctaag gactttttct ttgaggtagg aattgatcct
    20041 aaagttgaag caaagcaaaa agaaaaactc ttggaactag acaaagttaa aaaacaaatt
    20101 gaagactcaa tcaatggtga tgcatggcta ccagaaaaac cggaaggcga aaaaccagta
    20161 caaaatacta ataaagaatt acagttacaa gaactgaaca agaagtatca gatggccaaa
    20221 gaggcaattg aaagtgctac aactcttgac gatgttgaaa ctcagtttga taaatacaca
    20281 aaagttggag ataaagataa atacccggat tctttacgta accaatatac tcaaggagat
    20341 aaagataaag aaatagaaaa agctaaaaaa tcactgggtg atttaagcga taaagtaaat
    20401 ggaaaaattg aagaagataa gtggctatct gccgaagtta aaaaaaaaca acagcaagaa
    20461 ttagaagcgc gtaaacaaaa agtaaacgac tctcttaaag gatctgatag tttaaaatca
    20521 ttgagagaaa cagtggaaaa agcttcttct aaaaatcaga aaaaaccaga atcttttgaa
    20581 gatgtttatg ttcctggaaa tgaagaaact gaaaaaacaa aagttcgtga tatacttcaa
    20641 aaaacttatc aaaaaacaga gcaaaatatt gaaacagatc catggttgag tccggaacag
    20701 aaaaaagctc aaaaagaaaa cgctaaaaca agattagatg caggtttgaa agctgtcgaa
    20761 actacagaaa gcttagataa acttaaagaa gttgagagtg acttccttga taaagaaaaa
    20821 aatccagatt caattccaaa tcaacataaa gctggaactc ctgaagttgc tcgcaaaact
    20881 ttcttagata actttgaaaa agaagctaaa aaagagattg aatcaattaa aaatgacgtt
    20941 acattaacag atgctgaaaa agcaacagct aaagctaaag tagaagcaca acttcaagaa
    21001 gctaaaggta aggctaagga atcaaaatcg tttgatgatt tgaagaacat tcaagataag
    21061 ttcaattctg aattaccaca cacaccaggt aagccgctta aagatcaaca atctgatgct
    21121 attgcagaat tagagaagaa gcagcaagaa attgaaaaag ctattgaggg tgataaaaca
    21181 ttaccaagag acgaaaaaga gaaacaaatt gctgactcta aggaacgctt aaaatctgac
    21241 actcaaaaag ttaaagatgc taaaaatgct gatgctatta aaaaagtatt tgaagaaggg
    21301 aaagtgaata ttcctcaagc acatatccca ggtgatttga acaaggataa agaaaaactt
    21361 cttgcagagt tgaagcaaaa agcagctgat actgaaaaag cgattgattc cgataaaact
    21421 ttgacagcga aagaaaaaga agagcaaaaa gcgaaaacaa aagaagaact tcaaaaagca
    21481 acagaagctg ttggagcaat tgacaatcgt gaagaactag ataaaaaagt cccagaactt
    21541 aagaaagcta ttgaagacac tcacgttaaa ggtaatcttg aaggtgttaa gaataaggct
    21601 attgaagatc ttaaaaaagt tcatgacgaa acagttgcta agataaatgg tgatgatacc
    21661 cttgacaaag ctactaaaga agctcaagtg aaagaagctg acaaagcttt ggcagcaggt
    21721 aaagatgcga tcactaaagc agatgatgct gataaagtgg gtgcagctgt tacagagcac
    21781 acaccaaaaa ttaaagcagc acataaaact ggtgatctta aaaaagttca agaagaggct
    21841 aaccaagctc ttgacaaagc agctgaaaaa gaacgtggag aaatcaataa tgatatcaca
    21901 ttaacggcaa aagacaaaga acaacaactg aaagaagttg agacagctct tactaaagct
    21961 aaagataacg tgaaagctgc taagacagca gacgctatca atgacgcacg tgataaaggc
    22021 gtagcaacaa ttgatgccgt tcataaagca ggtcaagact taggtgctcg taagtcaggt
    22081 caagtcgcta aacttgaaga agcagctaaa gcaacgaaag acaagatttc agctgatcca
    22141 actttgacaa gcaaagaaaa agaagagcaa tctaaagctg ttaacgctga acttaagaaa
    22201 gcgatagaag ctgttaacgc agctgacaca gctgacaagg ttgacgatgc tcttggtaaa
    22261 ggtgtcacag acatcaagaa ccaacataag acaggtgacc ctgtcgtagc tcgtcgtgag
    22321 gctcatggta agcaacttga tagagtcgct caagaaacta aagatgcgat tgaaaaagac
    22381 cctactttga cgactgaaga aaaagctaaa caagttaaag acgtagatgc cgctaaagaa
    22441 agaggcatgg ctaagcttac tgaagctaag aatgctgatg agttagacaa agcttacggt
    22501 gaaggtgtta cagacatcaa gaaccaatac aagtcaggtg accctatcga agctcgtcgt
    22561 ggattacata acaagtcaat cgacaaagtg gcgcaagcaa ctaaggacgc tatcacagca
    22621 gatacgactt tgactgaagc cgaaaaagaa acacaacgtg gcaatgttga taaagaagca
    22681 actaaagcta aagaagaact tgctaaggct aaagatgctg atgctttaga caaagcgtac
    22741 ggtgacggtg tgaccagcat caagaaccaa cacaagtcag gtaaaggtct tgacgttcgt
    22801 aaagatgagc acaagaaagc tcttgaagct gttgctaaac gtgtcactgc tgaaattgag
    22861 gctgatccaa ccttaacacc agaagtgaga gaacaacaaa aagcagaggt tcaaaaagag
    22921 cttgaacttg cgactgataa gattgctgaa gctaaagatg cagatgaagc agacaaagct
    22981 tacggtgatg gtgtcacagc gatcgaaaat gcccacgtta ttggtaaagg tatcgaagct
    23041 cgtaaagacc ttgctaagaa agaccttgct gaagctgttg ctaagacaaa agctctcatt
    23101 attgaagaca aaacgcttac tgatgatcaa cgtaaagaac agttatcagg tgttgataca
    23161 gagtatgcta aaggtatcga gaatattgat gcagctaagg atgctgcagg tgttgataaa
    23221 gcatatagtg acggtgttcg tgacatcctg gcacagtaca aagaaggtca aaaccttgat
    23281 gatcgtcgta atgctgccaa agaatttctt cttaaagaag cagacaaagt gacgaaacta
    23341 atcaatgatg atccaacctt gactcatgac caaaaagttg atcaaatcaa caaagttgaa
    23401 caagctaagt tagacgcaat caagtctgtt gatgatgctc aaacagctga tgctatcaat
    23461 gatgctcttg gtaagggtat tgaaaacatc aacaaccaat accaacatgg cgatggcgtt
    23521 gatgttcgta aagcgactgc caaaggcgat cttgaaaaag aagctgctaa agtgaaagct
    23581 cttattgcta aggatccaac cttgactcaa gctgataaag acaaacaaac agcagcggtt
    23641 gacgcagcta agaacacagc aattgcagcg gttgataaag cgacaacagc tgaaggcatt
    23701 aaccaagaac ttggtaaagg catcacagct atcaataaag cttaccgtcc aggtgaaggt
    23761 gttaaagcac gtaaagaagc cgctaaagct gatcttgaaa aagaagctgc taaagtgaaa
    23821 gctcttatta ctaacgaccc aaccttaaca aaagctgata aagctaaaca aacaggagct
    23881 gttgctaaag c
//
LOCUS       DAUDYX010000031        23400 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.031, whole genome shotgun sequence.
ACCESSION   DAUDYX010000031 DAUDYX010000000
VERSION     DAUDYX010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23400
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.031"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            137..1372
                     /gene="arcA"
                     /locus_tag="VHD02_001189"
     CDS             137..1372
                     /gene="arcA"
                     /locus_tag="VHD02_001189"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEQ5497799.1"
                     /translation="MTAQTPIHVYSEIGKLKKVLLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRDEGIEVLYLETLAAESLVTPEIREAFIDEYLSEANIRGRA
                     TKKAIRELLMAIEDNQELIEKTMAGVQKSELPEIPASEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGTGVSLNHMFSETRNRETLYGKYIFTHHPIYGGGKVPMVYDRNETTR
                     IEGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHL
                     DTVFTMVDYDKFTIHPEIEGDLRVYSVTYDNEELHIVEEKGDLAELLAANLGVEKVDL
                     IRCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKIHGSEL
                     VRGRGGPRCMSMPFEREDI"
     gene            1411..1764
                     /locus_tag="VHD02_001190"
                     /pseudo
     CDS             1411..1764
                     /locus_tag="VHD02_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            1804..2817
                     /gene="argF"
                     /locus_tag="VHD02_001191"
     CDS             1804..2817
                     /gene="argF"
                     /locus_tag="VHD02_001191"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEQ5497800.1"
                     /translation="MTQVFQGRSFLAEKDFTRAELEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVKENFGKLEGLTLVY
                     CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEEEIVTLAEGYAKESGARILITEDA
                     DEAVKGADVLYTDVWVSMGEEDKFKERVELLQPYQVNMDLVQKAGNDKLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            2987..4480
                     /locus_tag="VHD02_001192"
     CDS             2987..4480
                     /locus_tag="VHD02_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEQ5497801.1"
                     /translation="MTEEKKRGFRIPSSYTVLFIIIAIMAVLTWFIPAGAYETAKGGG
                     VISGTYKTVASNPQGFFDILMAPVRGMLGVEGTDGAIQVSFFILMVGGFLGVVNKTGA
                     LDTGIASVVRKNKGREKMLIAILIPLFALGGTTYGMGEETMAFYPLLIPVMIAVGFDS
                     IVAVAIILIGSQIGCLASTINPFATGVAADAAGVSIADGMIWRVIQWVILVGMSIWFV
                     YNYASKIEEDPSKSLVADKEEEHKELFQLQNSGEDLNKRQRNVLTIFTLTFVIMILSL
                     IPWEDFGIKFFTNINTWLTTMPILGGVIGKTMGAFGTWYFPEITMLFIMMGVLVAIVY
                     RMSEEDFFSSFLTGAGEFLGVAMICAIARGIQVIMNGGMITATILHLGETSLSGLSSQ
                     VFVILAYIFYLPMSFLIPSTSGLAGATMGIMAPLGQFSNVPAHLVITAFQSASGILNM
                     ISPTSAIVMGALALGRVDLGTWWKFIGKFIVMVMLVSVLLLVVATFF"
     gene            4497..5828
                     /locus_tag="VHD02_001193"
     CDS             4497..5828
                     /locus_tag="VHD02_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEQ5497802.1"
                     /translation="MESYITPKHQEACVAAIKQIVSYPSVCHEGENGTPFGQAIQDVL
                     EATLDLCQGLGFYTYIDPKGYYGYAELGDQKEVLAILCHLDVVPEGDRQLWKTEPFDC
                     VEAGGCLFGRGTQDDKGPTMMALFATKALIDAGVTFNKRIRFIFGTDEETLWRCMNRY
                     NDVEEQATFGFAPDSSFPLTYAEKGLLQAKLVGKGSDKLSLEIGQAYNVVPARASYQG
                     DKLETLQKELDKLGFEYVVKEGELTVYGLAQHAKDAPDGINAMIRLAKALVVLEPNKT
                     LDFLANVIDEDGRALNIFGRIEDEPSGKLSFNAAGLTLTKDKAEIRLDIRIPVLADKE
                     KLVQQLSQKAQEYGLTYEEFDYLAPLYVPLDSELVTTLLSVYRKKTGDQSPAQSSGGA
                     TFARTMKNCVAFGALFPDAVQTEHQENEHIVLADAYRAMDIYAEAIYRLTQ"
     gene            5848..6798
                     /gene="arcC"
                     /locus_tag="VHD02_001194"
     CDS             5848..6798
                     /gene="arcC"
                     /locus_tag="VHD02_001194"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEQ5497803.1"
                     /translation="MTKQKIVVALGGNAILSTDASAKAQQEALMSTSKSLVKLIKEGH
                     EVIVTHGNGPQVGNLLLQQAAADSEKNPAMPLDTCVAMTEGSIGFWLVNALDNELQAQ
                     GIQKEVAAVVTQVIVDAKDPAFENPTKPIGPFLTEEDAKKQMAESGASFKEDAGRGWR
                     KVVPSPKPVGIKEANVIRSLVDSGVVVVSAGGGGVPVVEDATSKSLTGVEAVIDKDFA
                     SQTLSELVDADLFIVLTGVDNVYINFNKPDQAKLEEVTVSQMKEYITQDQFAPGSMLP
                     KVEAAIAFVENKPNAKAIITSLENIDNVLSANAGTQIIAG"
     gene            7112..8104
                     /locus_tag="VHD02_001195"
     CDS             7112..8104
                     /locus_tag="VHD02_001195"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEQ5497804.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLIAKLDVVEVQGPILSRVGDGM
                     QDNLSGTENPVSVNVLKIPNATFEVVHSLAKWKRHTLARFGFNEGEGLVVNMKALRPD
                     EDSLDQTHSVYVDQWDWEKVIPDGKRNLAYLKETVETIYKVIRLTELAVEARYDIEAV
                     LPKKITFIHTEELVAKYPDLTPKERENAITKEFGAVFLIGIGGVLPDGKPHDGRAPDY
                     DDWTTETENGYHGLNGDILVWNDQLGSAFELSSMGIRVDEEALKRQVEMTGDQDRLAF
                     DWHKSLLNGLFPLTIGGGIGQSRMVMFLLRKKHIGEVQTSVWPQEVRDSYDNIL"
     gene            8227..8766
                     /gene="rsmD"
                     /locus_tag="VHD02_001196"
     CDS             8227..8766
                     /gene="rsmD"
                     /locus_tag="VHD02_001196"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEQ5497805.1"
                     /translation="MRVVSGEFGGRPLKTLDGKITRPTSDKVRGAIFNMIGPYFNGGR
                     VLDLFAGSGGLAIEAVSRGMSAAVLVEKNRKAQAIIQDNIIMTKAENRFTLLKMEAER
                     AIDCLTGRFDLVFLDPPYAKETIVATIEALAAKNLLSEQVMVVCETDKTVLLPKEIAT
                     LGIWKEKIYGISKVTVYVN"
     gene            8756..9247
                     /gene="coaD"
                     /locus_tag="VHD02_001197"
     CDS             8756..9247
                     /gene="coaD"
                     /locus_tag="VHD02_001197"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEQ5497806.1"
                     /translation="MLTKIGLYTGSFDPVTNGHLDIVKRASGLFDQIYVGIFDNPTKK
                     SYFKLEVRKAMLTQALADFTNVIVVTSHERLAIDVAKELRVTHLIRGLRNATDFEYEE
                     NLEYFNHLLAPNIETVYLISRNKWQALSSSRVRELIHFQSSLEGLVPQSVIAQVEKMN
                     EKT"
     gene            9234..10271
                     /locus_tag="VHD02_001198"
     CDS             9234..10271
                     /locus_tag="VHD02_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEQ5497807.1"
                     /translation="MKRLNKIKWWLVGLLALISLLLALFFPLPYYIEMPGGAYDIRTV
                     LQVNGKEDKRKGAYQFVAVGISRASLAQLLYAWLTPFTEISTAEDTTGGYSDADFLRI
                     NQFYMETSQNAAIYQALSLAGKPVTLDYKGVYVLDVNNESTFKGTLHLADTVTGVNGK
                     QFTSSAELIDYVSHLKLGDEVTVQFTSDNKPKKGVGRIIKLKNGKNGIGIALTDHTSV
                     NSEDTVIFSTKGVGGPSAGLMFTLDIYDQITKEDLRKGRTIAGTGTIGKDGEVGDIGG
                     AGLKVVAAAEAGADIFFVPNNPVDKEIKKVNPNAISNYEEAKRAAKRLKTKMKIVPVT
                     TVQEALVYLRK"
     gene            10676..11653
                     /locus_tag="VHD02_001199"
     CDS             10676..11653
                     /locus_tag="VHD02_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ5497808.1"
                     /translation="MVTIKQVAEEAGVSRSTVSRYISQKGYVSDDARHKIKAAIAKLH
                     YTPNVLAQSLKTKKNQLVGLLLPDISNPFFPRLARGAEEYLKEKGYRVMLGNISDSEA
                     LEEEYVHVLLQSNAAGIITTHDFTKRYPTLAIPVVVVDRVDQETQYGVFSDNRAGGLL
                     AAQTVWQAGAKEVLLIRGPLDNAENINERFEASFSYLQKQDVTMYVCDSQNFDFENIQ
                     LEASSNLKCYPTIDSIIAPSDIHAIAYIHELHSQGKKIPQDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYLMGRYAAELVYTIASQLTVKANRIKLPVHYVERKTIRKR"
     gene            12077..12721
                     /locus_tag="VHD02_001200"
     CDS             12077..12721
                     /locus_tag="VHD02_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEQ5497809.1"
                     /translation="MDFSERCQMKKEISPEMYNYNKFPGPKFIHFEEQVKTEGIDLLL
                     LEDVKNAFDTTSFGQRYTEVLLKYDYIVGDWGNEQLRLKGFYKDSDDIKKTNRISRLE
                     DYIKEFCNFGCAYFVLENLHPQDIKFEEERQPRRKKSPKSKSNRRKPNYSNQQPATPK
                     SKSKRGSKEKQPENQAFTSQKRRSNTKHKEKSKRNQTSQLNTKISHFIIRKKDK"
     gene            12725..13804
                     /gene="rlmN"
                     /locus_tag="VHD02_001201"
     CDS             12725..13804
                     /gene="rlmN"
                     /locus_tag="VHD02_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEQ5497810.1"
                     /translation="MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEE
                     MTNISKDFVSILNDSFCVNPLKQRVVQESADGTVKYLFELPDGMLIETVLMRQHYGHS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVM
                     GIGEPFDNYKNVMCFLRVINDDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVS
                     LHAPNNDLRSRIMRVNRSFPLEKLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQE
                     LADLTKTIRKLSYVNLIPYNPVSEHDQYSRSLKERVLAFYDVLKKNGVNCVVRQEHGT
                     DIDAACGQLRSKTMKKDREKVTATK"
     gene            complement(13886..14512)
                     /locus_tag="VHD02_001202"
     CDS             complement(13886..14512)
                     /locus_tag="VHD02_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEQ5497811.1"
                     /translation="MIAFFSICSGILLTHLFYCLLLYFPDNGQEQLSKQTVIVCDQLT
                     FAKRVFRQFQNHFITSQKIFFSLEISLIIILWASMVHWVSASYCYLLLFSLLFSLFDW
                     RSQEYPFILWLFSFVSLLLFYSINYLSLILLLLGLLAHLRPFSIGAGDFFYLASLALV
                     LDLTSLIWLIQLASLAGITACLLLGIKRIPFIPYLSFGLFWIVLLEHF"
     gene            14740..15267
                     /locus_tag="VHD02_001203"
     CDS             14740..15267
                     /locus_tag="VHD02_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEQ5497812.1"
                     /translation="MTNTLVENIYASVTHNISKKEASKNEKTKAVLNQAVADLSVAAS
                     IVHQVHWYMRGPGFLYLHPKMDELLDSLNANLDEMSERLITIGGAPYSTLAEFSKHSK
                     LDEAKGTYDKTVAQHLARLVEVYLYLSSLYQVGLDITDEEGDAGTNDLFTAAKTEAEK
                     TIWMLQAERGQGPAL"
     gene            15409..15612
                     /locus_tag="VHD02_001204"
     CDS             15409..15612
                     /locus_tag="VHD02_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEQ5497813.1"
                     /translation="MKTLYDVQQLLKNFGIFVYLGKRLYDIEMMKIELQRLYDSGLLD
                     KRDYLNAELILRREHRLELEKEG"
     gene            15617..16588
                     /locus_tag="VHD02_001205"
     CDS             15617..16588
                     /locus_tag="VHD02_001205"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEQ5497814.1"
                     /translation="MSQKLLGIDLGGTTIKFGILTAAGEVQEKWAIETNILEGGKHIV
                     PDIVASIKHRLDLYGLSSADFVGIGMGSPGAVDRDTNTVTGAFNLNWKETQEVGSVVE
                     KELGIPFAIDNDANVAALGERWVGAGENNPDVVFMTLGTGVGGGIIADGNLIHGVAGA
                     GGEIGHMIVEPENGFACTCGSHGCLETVASATGVVKVARLLAEAYEGDSAIKAAIDNG
                     EGVTSKDIFMAAEAGDSFADSVVEKVGYYLGLASANISNILNPDSVVIGGGVSAAGEF
                     LRSRIEKYFVTFTFPQVRYSTKIKIAELGNDAGIIGAASLARQFITK"
     gene            16598..16987
                     /locus_tag="VHD02_001206"
     CDS             16598..16987
                     /locus_tag="VHD02_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEQ5497815.1"
                     /translation="MSPITLILWLLLVGIVGYYTWNYFSFRKMAKQVDNETFKDVMRQ
                     GQLIDLREPAAFRAKHILGARNFPAQQFDAAIKGLRKDKPVLIYENMRPQYRVPAVKK
                     LKKAGFEDVYVLKDGIDYWDGKVKQTT"
     gene            17169..19010
                     /gene="typA"
                     /locus_tag="VHD02_001207"
     CDS             17169..19010
                     /gene="typA"
                     /locus_tag="VHD02_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEQ5497816.1"
                     /translation="MTNLRNDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELQER
                     AMDSNDLEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPAEVVDEVLELFIELGADDEQ
                     LEFPVVYASAINGTSSLSDDPADQEHTMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDCVEALPILRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVKCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            19132..19383
                     /locus_tag="VHD02_001208"
     CDS             19132..19383
                     /locus_tag="VHD02_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEQ5497817.1"
                     /translation="MFYLIMAVLIISYYLYMAPKSVRNTLGMIGLVGLVALLIVLAGL
                     SFIKIMQIPPEFFIGMGMVALGYFALKDVRKMTKKPRVK"
     gene            19739..21097
                     /locus_tag="VHD02_001209"
     CDS             19739..21097
                     /locus_tag="VHD02_001209"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEQ5497818.1"
                     /translation="MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFD
                     QNPAAQALLEEGIKVICGSHPVELLDENFEYMVKNPGIPYDNPMVKRALAKEIPILTE
                     VELAYFVSEAPIIGITGSNGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIA
                     GDTLVMELSSFQLVGVNAFRPHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESD
                     YLILNANQEISATLAKTTKATVIPFSTQKVVDGAYLNDGILYFKEQAIIAATDLGVPG
                     SHNIENALATIAVAKLSGIADDIIAQCLSHFGGVKHRLQRVGQIKDITFYNDSKSTNI
                     LATKKALSGFDNSRLILIAGGLDRGNEFDDLVPDLLGLKQMIILGESAERMKRAANKA
                     EVSYLEARNVAEATELAFKLAQTGDTILLSPANASWDMYPNFEVRGDEFLATFDCLRG
                     DA"
     gene            21097..22179
                     /locus_tag="VHD02_001210"
     CDS             21097..22179
                     /locus_tag="VHD02_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEQ5497819.1"
                     /translation="MPKKILFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     TEIEKSGLDVTFHAIATGKLRRYFSWQNLADVFKVALGLLQSLFIIAKLRPQALFSKG
                     GFVSVPPVVAAKLLGKPVFIHESDRSMGLANKIAYKFATTMYTTFEQEDQLSKVKHLG
                     AVTKVFKDANQMPESTQLEAVKEYFSRDLKTLLFIGGSAGAHVFNQFISDHPELKQRY
                     NIINITGDPHLNELSSHLYRVDYVTDLYQPLMAMADLVVTRGGSNTLFELLAMAKLHL
                     IVPLGKEASRGDQLENATYFEKRGYAKQLQEPDLTLHNFDQAMADLFEHQADYEATML
                     ATKEIQSPDFFYDLLRADISSAIKEK"
     gene            22180..23328
                     /locus_tag="VHD02_001211"
     CDS             22180..23328
                     /locus_tag="VHD02_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEQ5497820.1"
                     /translation="MAKDKEKQSDDKLVLTEWQKRNIEFLKKKKQQAEEEKKLKEKLL
                     SDKKAQQQAQNASEAVELKTDEKTDSQEIESETTSKPKKPKKVRQPKEKSATQIAFQK
                     SLPVLLGALLLMAVSIFMITPYSKKKEFSVRGNHQTNLDELIKASKVKASDYWLTLLT
                     SPGQYERPILRTIPWVKSVHLSYQFPNHFLFNVIEFEIIAYAQVENGFQPILENGKRV
                     DKVRASELPKSFLILNLKDEKAIQQLVKQLTTLPKKLVKNIKSVSLANSKTTADLLLI
                     EMYDGNVVRVPQSQLTLKLPYYQKLKKNLENDSIVDMEVGIYTTTQEIENQPEVPLTP
                     EQNAADKEGDKPGEHQEQTDNDSETPANQSSPQQAPPSPETVLEQAHG"
BASE COUNT         7459 a         4128 c         4893 g         6920 t
ORIGIN      
        1 atgtaagcga ttactcaaaa taactgtgag aaatacccta tttttatctt ttttaaggag
       61 tacactagga atataagcaa gagttaagaa tcttgacggt gaattcacaa aaaccaatta
      121 gaaagaggta attcttatga ctgctcaaac accaattcat gtttattctg aaattggaaa
      181 attaaaaaaa gtgttgctcc acagaccagg taaagaaatt gaaaatctta tgccagacta
      241 cttggaacgc ttgttgtttg atgacattcc attcttagag gatgcccaaa aagaacatga
      301 tgcttttgcc caagctttgc gtgacgaagg tattgaagtc ttgtaccttg agaccttagc
      361 tgcagaatct cttgtcactc cagaaattcg cgaagctttc attgatgaat acctaagtga
      421 agctaatatc cgtggaagag ctactaaaaa agccattcgt gagttgttga tggctatcga
      481 agacaatcaa gaattgattg aaaaaaccat ggcaggggtt caaaaatctg aacttccaga
      541 gatcccagca tctgaaaaag gcttgactga cttggttgaa tccaattacc catttgccat
      601 cgacccaatg ccaaaccttt atttcacacg ggacccattc gcaactattg gtacaggtgt
      661 ttctctcaac cacatgttct cagaaacccg taaccgtgaa accctttatg gtaaatacat
      721 tttcactcat cacccaattt atggtggtgg caaagtgcct atggtttatg accgtaatga
      781 aaccactcgc attgaaggtg gggacgaact tgttctttca aaagatgtgc ttgcggttgg
      841 tatttctcaa cgtacagatg ctgcttctat tgaaaaattg ttggttaaca tctttaaaca
      901 aaaccttggc ttcaagaaag tattggcctt tgaatttgca aataaccgta aattcatgca
      961 cttagacact gtctttacca tggttgacta tgacaaattc accattcacc cagaaattga
     1021 aggcgatctt cgcgtttact ctgtgactta tgacaacgaa gaacttcata ttgttgaaga
     1081 aaaaggtgat ttagcagaac ttcttgctgc taaccttggt gttgaaaaag ttgaccttat
     1141 ccgttgtggt ggtgacaact tagtagcagc aggtcgtgaa caatggaacg atggttctaa
     1201 cacccttact atcgcaccag gtgtggttgt ggtttataac cgtaacacca ttaccaatgc
     1261 cattcttgaa tctaaaggct tgaaattgat caagattcac ggaagtgaat tggttcgcgg
     1321 tcgtggtgga cctcgttgta tgtcaatgcc atttgaacgt gaagatattt aataagctat
     1381 ggtaaaggtg gttataggtc agaagccttt tcaacaggac aatttttagc tgaaacgaaa
     1441 atagaagcac gattgacacg cattgtaacc ttagcagatt attgtggttg cggttatggt
     1501 gccaaagtca ctgaagcgct agaaacttat accagacgag aaggctttta ccaactaacc
     1561 attcacagtg aactgacagc acaaaccttt tatgaaaact taggttatca gacctatggt
     1621 cccaagtgtt tagaagatgg tgagtattgt cagtcccttg caaaaaccat tcttaaatgg
     1681 gagaagaata tggacatagc aatgctaatt gcgattgttg gtggtctatt aggctgctat
     1741 ctctatctca caaaaaataa ttaatcaaaa cagattacag ttaacactcg aaggagacaa
     1801 tagatgacac aagtatttca aggacgtagc ttcttagcag aaaaagattt tacacgcgct
     1861 gaattagaat accttattga tttttcagca catttgaaag atttgaaaaa acgtggtgtg
     1921 cctcatcact acttagaagg taaaaacatt gccctcttgt ttgaaaaaac atcaactcgt
     1981 acgcgtgcag cttttacaac agcagccatt gacctaggtg ctcacccaga atacctcggt
     2041 gccaatgaca tccaacttgg taaaaaagaa tcaacagaag acactgctaa agtattgggt
     2101 cgtatgtttg atgggattga attccgtggt ttcagccaac gcatggttga agaattggca
     2161 gaattctcag gtgtgccagt ttggaatggt ttgactgatg aatggcaccc aacccaaatg
     2221 cttgctgact atttaacagt gaaagaaaac tttggcaaac ttgaaggcct tacccttgtt
     2281 tactgtggtg atgggcgtaa taacgttgcc aattcccttc ttgtgactgg tgctatcctt
     2341 ggtgttaatg ttcatatctt ctcaccaaaa gaactcttcc cagaagaaga gattgtgaca
     2401 ttggctgaag gctatgctaa agaaagtggc gctcgtatcc ttatcacaga agatgctgac
     2461 gaagctgtta agggcgcaga cgtcctttac acagacgttt gggtatcaat gggtgaagaa
     2521 gacaaattca aagaacgtgt tgaattgctt caaccatatc aagtcaacat ggaccttgtt
     2581 caaaaagcag gaaatgacaa gcttatcttc ttgcactgct tgccagcttt ccatgacaca
     2641 aataccgttt atggtaaaga cgttgctgaa aaattcggcg tgaaagaaat ggaagtcact
     2701 gatgaagtct tccgcagcaa atacgctcgt cattttgacc aagctgaaaa ccgtatgcac
     2761 actatcaaag cggttatggc agcaaccctt ggtaacctct ttattccaaa agtgtaataa
     2821 ggactggtac tccttaactc atttcacata tcaatgtgac ccctttaatt taacaattta
     2881 actacttaaa aacgtacttt aactacatga caagtcgttc tggttttcgc gtccttgacc
     2941 ggaactagaa cggcttattt ttatgaaaag gtaagaggta atcattatga cagaagaaaa
     3001 aaaacggggt tttagaattc cttcttctta caccgttctt tttatcatta tagccatcat
     3061 ggcagtgtta acttggttta ttccagctgg cgcctacgaa accgctaagg gcggtggtgt
     3121 tatttcagga acctacaaaa cagtagcatc caatcctcaa ggattttttg acatcttgat
     3181 ggctcccgtt cgaggcatgt taggtgttga aggcactgat ggtgctattc aagtgtcttt
     3241 ctttatctta atggttgggg gctttttagg agttgtcaac aaaacaggtg ctcttgacac
     3301 agggattgcg tcagtggttc gtaaaaacaa aggtagagaa aaaatgttga ttgctatttt
     3361 aatccctttg tttgctctag gtggaacgac ctatggtatg ggggaagaaa ccatggcctt
     3421 ttacccactt ttaattcctg ttatgattgc ggttggtttt gacagtattg ttgctgtagc
     3481 cattattttg attggttctc aaattgggtg tctagcctcc actattaacc catttgcaac
     3541 tggtgtagca gctgatgctg ctggtgtcag cattgcagat ggaatgattt ggcgtgttat
     3601 ccaatgggtt attcttgtcg gcatgtctat ttggtttgtt tacaattacg ctagtaagat
     3661 tgaagaagac ccaagtaaat cattggttgc agacaaggaa gaagagcata aggaactctt
     3721 ccagttgcaa aattctggag aagacttaaa caagcgccaa cgaaacgttt tgaccatttt
     3781 tactttgacc tttgtcatta tgattcttag tttgattccg tgggaagatt ttggcatcaa
     3841 attctttact aatatcaata cttggttaac cacaatgcct atcttagggg gagttattgg
     3901 gaaaaccatg ggagcatttg gtacttggta tttcccagaa atcaccatgc tctttatcat
     3961 gatgggtgtc ttagttgcta ttgtttatcg tatgagtgaa gaagactttt ttagctcttt
     4021 tttgactggt gcaggtgaat ttttaggtgt tgccatgatc tgtgctattg cacgcggtat
     4081 tcaagttatc atgaatggtg gtatgattac agccactatc ttacacttag gtgaaacaag
     4141 tctttctggt ttatcttctc aagtttttgt gatattagct tatattttct acctcccaat
     4201 gtccttcttg attccatcaa catcaggact tgctggggct acaatgggga ttatggcacc
     4261 gcttggacaa ttctcaaatg tccctgctca ccttgttatt acagccttcc agtcagcttc
     4321 tggaatctta aacatgattt ctccaacttc agcaatcgtt atgggagcac ttgcgcttgg
     4381 tcgcgttgac cttggtactt ggtggaaatt cattggtaaa tttattgtaa tggtgatgct
     4441 tgtaagcgtg ctattacttg tagttgcaac attcttttaa cagataggag gttcctatgg
     4501 aatcctatat cacacctaaa catcaagagg cctgtgtagc tgccattaaa cagatcgttt
     4561 cttacccttc tgtttgtcat gaaggagaaa atggcacacc ttttggacag gccattcaag
     4621 atgttctcga agcaacctta gacttatgtc aaggtcttgg cttctatact tatattgatc
     4681 ctaaaggtta ctatggctat gctgagttag gtgaccaaaa agaggtcttg gctattctgt
     4741 gccatttgga cgttgttcca gaaggagatc gtcagttatg gaagacagag ccttttgact
     4801 gtgttgaggc aggcggctgt ttatttggtc gaggcactca agacgataaa gggcctacta
     4861 tgatggcact atttgccacc aaagccttga tagatgcagg cgttaccttt aataaacgca
     4921 ttcgttttat tttcggaaca gatgaagaaa ctttgtggcg gtgtatgaat cgctacaatg
     4981 atgtagaaga acaggctacc tttgggtttg ctcctgattc aagtttccct ttaacctatg
     5041 ccgaaaaagg cctacttcag gcaaaattag tggggaaagg ttctgataaa ctgtcacttg
     5101 aaatcggaca agcctacaat gttgttcctg caagagcctc ttatcaaggt gataagcttg
     5161 agactttaca aaaagaacta gataagcttg gctttgagta cgtcgtcaaa gagggagaat
     5221 taacggttta tggtctcgcc caacatgcta aagatgctcc cgatggcatc aacgctatga
     5281 ttaggcttgc caaagcttta gtggtccttg aacctaacaa aacccttgat tttcttgcta
     5341 atgtcattga tgaagatggc agagcactta atatttttgg cagaatcgaa gatgaacctt
     5401 caggcaaatt aagctttaat gccgcagggc ttaccttaac caaagacaag gcagaaattc
     5461 gcctagatat tcggattcca gttttggcag ataaggaaaa attagtccaa caattaagcc
     5521 aaaaagcaca agaatacggc ctaacctatg aagaatttga ttatttggcc ccattatatg
     5581 tgccattaga cagtgaattg gtaacaacct tgttatctgt ctatcggaaa aaaacaggtg
     5641 atcaaagccc tgcccaatca tcaggaggag caacctttgc tagaacaatg aagaattgtg
     5701 ttgcctttgg tgcccttttc cctgatgctg ttcaaacgga acaccaagaa aatgaacata
     5761 ttgttttagc ggatgcttac cgcgctatgg atatttatgc ggaagctatt taccgactaa
     5821 cgcaataaca agatatgagg agtaattatg acgaaacaaa aaatcgtagt cgcacttgga
     5881 ggaaatgcta ttctttcaac agatgcgtca gcaaaagcac aacaagaagc cttgatgtcc
     5941 acatcaaaat cattggttaa attaatcaaa gaaggtcatg aagtcatcgt gacccacggc
     6001 aatggcccac aagttggtaa tctcttatta caacaagcag cggcagattc tgaaaagaat
     6061 ccggctatgc ctttggatac ttgtgttgct atgacagaag gtagcatcgg tttttggtta
     6121 gttaatgccc ttgataatga attgcaagcc caaggtattc aaaaagaagt ggcagctgtt
     6181 gtcactcaag tgatcgtgga tgccaaagac ccagcttttg aaaacccaac aaaaccaatt
     6241 ggtccatttc ttaccgaaga agatgccaaa aaacaaatgg cggagtctgg tgcaagtttc
     6301 aaagaagatg caggtcgtgg ctggcgtaag gttgtgccat caccaaaacc agttggtatt
     6361 aaagaagcca atgtcatcag aagtctcgtt gattctggcg ttgttgtggt tagtgctggt
     6421 ggtggcggtg tgcctgttgt ggaagatgca actagcaagt ccctgactgg tgtggaagct
     6481 gttattgaca aggactttgc tagccaaacc ctttcagaat tggtggatgc agacctcttt
     6541 atcgttttga caggagttga caatgtttac atcaacttca acaaacctga ccaagccaaa
     6601 ttggaagaag tgactgtctc tcaaatgaaa gaatacatca cacaagacca atttgcacca
     6661 ggtagtatgt taccaaaagt ggaagctgcc attgcttttg ttgaaaacaa accaaatgct
     6721 aaagccatta tcacatcgct cgaaaatatt gacaatgtac tatcagcaaa cgctggaaca
     6781 caaattatcg cagggtaatc aagaggattt ttagttctgt ttaaaagact catttaatgg
     6841 tattttctga agagtacgac tgacataaag agttgatact gtttttaaag gaaaagagtg
     6901 tgccataata aaaaacaata atagtattag agaagactaa ttttattgag aattagcctt
     6961 ttcttttggc tttgataggt gtataataac catactgttt ttttattttt caaaatatta
     7021 gaataaacaa aagtggacaa cgtataaaga gaactgctaa gtcgttgagt tcattagtcc
     7081 caaaaagatt accaaaaatg aaggaggagc tatgaagaaa agttttattc atcaacaaga
     7141 ggagatttcc tttgttaaga atacgttcac tcagtattta attgctaagt tagatgttgt
     7201 tgaagtgcaa ggacccattt taagtcgggt cggggatggt atgcaagata atttgtccgg
     7261 tactgaaaat ccagtatctg tcaatgtctt gaaaattcct aatgcaacct ttgaagttgt
     7321 gcattcatta gcaaaatgga aacgtcacac cttagcccgt tttgggttta atgaaggtga
     7381 aggtcttgtt gttaatatga aggctctccg acctgatgag gattcacttg accaaaccca
     7441 ttctgtctat gttgaccaat gggactggga aaaagtcatc cctgatggca aacgtaatct
     7501 cgcttacctt aaagaaactg ttgaaaccat ttacaaggtc attcggctaa ctgaattagc
     7561 tgtagaagct cgttatgata ttgaggctgt tttacctaag aaaataacct tcattcatac
     7621 cgaagaattg gtagctaaat accctgattt gacacccaaa gaacgtgaaa atgccattac
     7681 taaagaattt ggagcagtct ttttaattgg tattggcggg gttttacctg acggtaaacc
     7741 acacgatggc cgcgctcctg actatgatga ctggacaaca gaaactgaaa atggctatca
     7801 tgggcttaat ggtgatattt tggtttggaa tgaccaactt ggttcagcct ttgaattatc
     7861 ctcaatgggg atccgtgtgg atgaagaggc cctaaaacgt caggtggaaa tgactggtga
     7921 ccaagaccgc ttagcctttg attggcacaa atctttgttg aatggacttt tccctctaac
     7981 gattggtgga ggaatcggtc aatcacgtat ggtcatgttt ttgttgcgta aaaaacatat
     8041 tggagaagtt cagacctccg tttggcccca agaagttcgt gatagctatg ataatatttt
     8101 ataagtaatc acatcatttt gctaagtttt ccacttgtat aagagacttt aaataaatga
     8161 tcagaaatat tgaaaaaaga aatcattttt aaggtttaga gctagaaatt tgctataata
     8221 aatgttatga gagttgtatc aggtgaattt ggtgggcgtc ctttaaagac gctcgatgga
     8281 aagataacac gccctacttc agacaaagtt agaggtgcga tttttaatat gataggacct
     8341 tactttaatg gtggtcgtgt tttagatttg tttgcaggtt caggtggatt agccattgag
     8401 gctgtttctc gtgggatgtc tgctgcagtt ttggttgaga agaatcgtaa agcgcaagct
     8461 attattcaag acaatattat aatgactaaa gctgagaatc ggtttacctt gttaaaaatg
     8521 gaagctgagc gagctattga ttgcttgact ggtcgatttg acttagtctt tttagaccct
     8581 ccttatgcca aagaaacaat tgtggcaact attgaagctt tagcagctaa aaacttattg
     8641 agtgaacagg ttatggttgt ctgtgagaca gataaaacag tcttgttgcc aaaagagatt
     8701 gcaacgttag gtatttggaa agaaaaaatt tatggaatta gtaaggtaac agtttatgtt
     8761 aactaaaata ggactttata cagggtcatt tgatcctgtt acaaacggtc atctcgatat
     8821 tgttaaacgg gcaagtggcc tatttgatca aatatatgtt gggatttttg ataatcctac
     8881 caaaaaaagc tattttaaac tagaagttcg taaagcaatg cttactcaag ctctggctga
     8941 ttttacaaat gtaattgtag taacttcgca cgaacgttta gctattgatg tagctaaaga
     9001 attacgagta actcacttga ttagaggctt gcgaaatgca actgactttg agtatgaaga
     9061 aaatttggaa tattttaatc accttttagc tccaaatatt gagaccgtct atttgatatc
     9121 aaggaataaa tggcaggccc ttagctctag tcgtgtgaga gagctaattc attttcagtc
     9181 ttccttagaa ggtttggttc ctcagtcagt tattgctcaa gtggagaaaa tgaatgaaaa
     9241 gacttaacaa aatcaaatgg tggttagtgg gtctgctagc tttaatctct ttgttgctag
     9301 cgttattttt tccgctacct tattatattg aaatgcctgg aggcgcttac gatattcgga
     9361 ctgtcttaca agtcaatggc aaagaagaca aacgaaaagg agcttaccag tttgttgcag
     9421 tgggcattag tcgtgccagc ctcgctcagc tattatatgc ttggctgaca ccgtttactg
     9481 aaattagtac agcagaagat acaacaggcg gatacagcga tgctgatttc cttcgaatta
     9541 atcaatttta catggaaaca tcacaaaatg cagctattta tcaagcttta tccttagctg
     9601 gaaaaccagt tacattagat tataaaggcg tatatgtttt agacgtaaac aacgaatcta
     9661 cttttaaagg aacgttacac ttagcagata ctgtaacagg tgtaaatggt aaacagttta
     9721 ctagttcagc agaacttatt gactatgttt ctcacctaaa actaggggat gaagttacgg
     9781 ttcagtttac gagtgataat aagcctaaaa aaggagttgg ccgtattatc aaactgaaaa
     9841 atgggaaaaa tgggattggc attgccttga ctgatcatac aagtgtcaat tcagaagaca
     9901 cagtgatctt tagtactaaa ggagtaggag gacctagtgc tggtctaatg tttactcttg
     9961 atatatatga tcaaataact aaagaagatt tacgcaaggg ccgtacaatt gcaggtacag
    10021 gaactattgg caaggatggc gaagtaggag atattggtgg tgcaggtctt aaagtagttg
    10081 cagcagctga agctggtgca gatatatttt ttgttccgaa taatcctgtt gataaggaaa
    10141 ttaaaaaagt taatccaaat gctataagta attacgaaga agccaaacgg gcagccaaac
    10201 gactaaagac caaaatgaag attgttcctg ttacgactgt tcaagaggca ctggtttatc
    10261 ttcgcaaata gaataactat gaatgtatta tattcacgct attaactcat aaaaaaacgg
    10321 ttgaaccaga tagacagatc cttgaccagt cttttgggtt caatcatttt ttacataaaa
    10381 catatctaac ttgacagcgt aaaaaagatg ttataaaagt ctagtggtaa aaaggcaata
    10441 gagaattgtg taaactttag tcgattatct ggaaatagag catactaaaa acacctagtg
    10501 aaaattgatt tttttcacta gctcatccaa gaactttcgc agataatcat gagtccattc
    10561 catgacttta ttattctgga aagccttgca atagacaagt taacgttata acaactaaat
    10621 taaaaacttg agattgaaaa atccccataa acttatttta caaggaggta aggctatggt
    10681 aaccattaaa caagttgctg aagaagcagg tgtttctaga tcaacagtct ccaggtatat
    10741 ttctcaaaaa ggatatgtat ctgatgatgc aaggcataaa atcaaagcag cgatagcaaa
    10801 attacattac actccaaatg tcttggcgca gtcattgaag acgaaaaaaa atcaactagt
    10861 cggtttatta ttaccagaca tttccaatcc tttttttccg aggttagcaa ggggagcaga
    10921 agaatattta aaagaaaagg ggtatcgggt tatgctagga aatatttcag acagcgaagc
    10981 tcttgaagaa gaatacgtcc atgtccttct tcaaagcaat gctgctggca ttattactac
    11041 ccatgacttt acaaagcggt atcctactct tgctattcca gttgtagtgg tagatcgtgt
    11101 tgaccaagag acccagtacg gtgtcttttc agataatagg gcagggggct tattggcggc
    11161 tcagactgtc tggcaagcag gggcgaaaga agttctgtta ataagaggtc cgctggataa
    11221 tgctgaaaac attaatgagc ggtttgaagc tagcttttct taccttcaaa aacaagacgt
    11281 gactatgtat gtgtgtgata gtcagaattt tgattttgaa aatattcagc tagaagctag
    11341 ttccaacctc aagtgttacc caacaataga cagtattata gcaccttctg atatccatgc
    11401 tattgcttat attcatgagc tgcattctca aggtaaaaag attcctcaag atgttcaaat
    11461 aataggttat gatgacattt taatgagtca atttatttat ccttctttat cgactattca
    11521 tcaatcctct tatttgatgg gccgttatgc cgccgaatta gtttatacta ttgctagtca
    11581 attaactgtg aaagcaaatc ggattaaatt gcctgttcac tacgttgagc gaaaaacaat
    11641 aaggaaaaga taagtaataa tattagtcca gattttagca ggtatgatga agatttattg
    11701 ggatttacct tctactttaa aagatgttat ctgtccttac tttcatcgct atcagtcaaa
    11761 acgcattctt atgcaactct taaccttaag cttaaggtct ttttgataac atagaggaat
    11821 gttacacgat gatgacgcaa taaagcctaa atatagtaat tgtcgtgatt ttatagctag
    11881 tgagtaatga gatagcaagt ctctcattta cttacaaaag tcaaaaaata gtgtataatg
    11941 gttgagttac agataacact gcaaaaaaga taacaagtgt ccataattat ctaagactat
    12001 aaaggcgata tagacagata taaaaaagat gacgaatcag ttatcactgg acaattaacc
    12061 gatttagtta caactaatgg atttttcaga aaggtgtcaa atgaaaaaag aaatttcgcc
    12121 agaaatgtac aactataata aatttccagg tcccaaattt attcattttg aagagcaagt
    12181 taagactgaa ggcattgatt tgttactctt agaagatgtt aagaacgctt ttgatacgac
    12241 tagctttggt caacgttata cagaagttct cctaaagtat gactatattg ttggcgattg
    12301 gggaaatgaa cagcttcgtc ttaaagggtt ttataaggat agtgacgata tcaagaaaac
    12361 gaatcgcatc tcacgtttag aagattatat taaagaattt tgcaattttg gttgtgctta
    12421 ttttgtttta gaaaatcttc atccacaaga tattaaattt gaagaggagc gccaaccaag
    12481 acgaaagaaa tcacctaaat caaaatcaaa tcgtcgcaag ccaaactatt caaatcagca
    12541 gcccgcaaca cctaagagta aatcgaagcg gggatcaaaa gaaaagcaac ctgaaaacca
    12601 agcgtttacc agtcaaaaac gtcgaagtaa tactaagcat aaagaaaagt caaaacgtaa
    12661 tcagactagt caacttaata ccaaaattag tcattttatc atcagaaaga aagataaata
    12721 atccatgaaa ccatcgattt atagcttaac acgcgatgaa ctgatcgcgt gggcagttga
    12781 acgcggccaa aaacagttta gagctactca aatttgggat tggctctata aaaaaagagt
    12841 tcaatctttt gaggagatga cgaatatttc taaagatttt gtttctattt taaatgatag
    12901 tttctgtgtc aatcctttaa aacagcgagt tgtacaagaa tcagctgatg gtacagtaaa
    12961 atatttattt gaactgcccg atggtatgtt aattgaaaca gttttaatgc gtcaacatta
    13021 tggtcattct gtttgtgtga cgactcaagt tggctgtaac attggctgta ctttttgtgc
    13081 tagtggattg atcaaaaagc aacgtgatct aaacagtggg gaaattacag ctcaaatcat
    13141 gttagtccaa aaatattttg acgatagaaa gcaaggtgag cgggttagcc atgttgtcgt
    13201 aatgggcatt ggtgaacctt ttgataatta caaaaatgtc atgtgctttc ttcgcgtcat
    13261 caacgatgat aatggcttag caattggtgc ccgtcatatt acagtctcta cctcagggtt
    13321 agctcacaag attcgggatt ttgcaaacga gggagtacag gttaatttag cagtctcgtt
    13381 gcatgcacca aataatgatt tacgttcaag gattatgcga gtaaatcgct cttttccgct
    13441 tgaaaagctc ttttcagcta tagaatacta tattgaaaaa acgaatcgtc gtgtcacttt
    13501 tgaatacatt atgctaaatg aagttaacga tagcattaag caagctcaag aattagcaga
    13561 cttaactaaa actattcgta agttatctta tgttaacttg attccgtata atcctgtttc
    13621 agaacatgat cagtatagta gaagtcttaa agagcgagtt ctagcgtttt atgatgtgct
    13681 aaagaaaaat ggagttaact gtgtggtgcg tcaagagcac ggaacagata ttgatgcagc
    13741 ctgtggccag ttacgttcta aaacgatgaa aaaagatcgc gaaaaggtta cagcgacaaa
    13801 ataaagccca aaaaatatct ttgattgaca atgcttgatg acaaaatatt cctagtcact
    13861 aaaatgacta ggttttttat ctggcctaaa aatgctctag aagcacaatc caaaataatc
    13921 caaaactaag ataaggaata aaaggtatgc gcttgatgcc taatagtaag caagcagtaa
    13981 tacctgctaa actagcaagt tgaattagcc aaatcaacga cgttagatcc aatactaaag
    14041 ctaagctagc aaggtagaaa aaatcgcctg ctccaattga aaaaggtctt aaatgagcta
    14101 ataaacctag tagcagtaat atcaatgata aataattgat cgaataaaac aggagtagac
    14161 ttacaaagct aaaaagccac aagataaaag ggtattcttg agaacgccaa tcaaataaac
    14221 taaataataa agaaaagagt aacaagtaac agtaactagc actaacccaa tgtaccatac
    14281 tagcccataa gataattatt agacttatct ctaagctaaa gaagattttt tggctagtta
    14341 taaaatggtt ttggaactgc cgaaaaacgc gtttggcaaa agttagttgg tcacaaacaa
    14401 tgacagtctg tttacttagc tgttcttgac cattatcagg aaaataaagt agtaaacagt
    14461 aaaaaagatg ggtcaataag atgcccgaac atatactaaa aaaagctatc atacttattt
    14521 attcgaaaag aaatcaaata cttgcaatct ttccttaatc attgtagaat atagatataa
    14581 aacgataatg attctaatta agtgtctaat catccaatta ggattattcc gttattgtcg
    14641 aataggatta ggtccttaac cctttcttat gtttatcacg ttagggttag ttccccctcc
    14701 attcttgccc tttaagctga aaagtaagga gatgttatta tgacaaacac actcgttgaa
    14761 aatatttatg cttcagtaac tcacaatatt tctaaaaaag aagcatcaaa aaatgaaaaa
    14821 actaaagctg ttttaaacca agctgtcgct gacttatctg tggctgcatc tattgtgcac
    14881 caagtacact ggtatatgcg tggtccaggt ttcttgtacc ttcacccaaa aatggatgaa
    14941 ttacttgata gcttgaatgc taatttagat gaaatgagcg agcgtttgat cactattggc
    15001 ggagcgcctt attctaccct agctgagttt tctaaacact ctaaattgga cgaagcaaaa
    15061 ggtacttacg ataaaacagt tgctcagcat ttagcaagat tggtcgaagt ttacctttac
    15121 ctcagcagcc tttaccaagt tggtttggat attaccgatg aagaaggcga tgctggaaca
    15181 aatgatttgt ttacagcagc taagacagag gctgaaaaaa ccatttggat gctccaagca
    15241 gagcgtggac aaggcccagc actctaattg attatgcctt aggataaaag aagtcaatga
    15301 taaaattggc ttctttttta tggaatcatc gattcatttt cagaaacata gaaagcgatt
    15361 ccatacttgt cttgaaattt gccgtagaat ttgctaaaat ctacttatat gaaaacacta
    15421 tatgatgtgc aacaattgct aaaaaacttt ggcatttttg tttaccttgg caaacgtctc
    15481 tatgatattg aaatgatgaa gattgaactt cagcgtctct atgatagcgg cttattggat
    15541 aagagggatt acttaaatgc ggagttaatt ttgcgacgtg agcatagatt agaattggaa
    15601 aaagaaggat agacaaatga gtcaaaaatt actggggatt gatttaggtg gtaccaccat
    15661 taaatttgga attttaacag cagcaggaga agtccaagaa aaatgggcaa ttgagactaa
    15721 tattttagaa ggaggcaagc atattgttcc cgatattgta gcttccatca aacatcgctt
    15781 agacttgtat ggtctaagca gtgctgattt tgtgggaatt ggtatgggat caccaggagc
    15841 ggttgaccgt gatactaata cagttactgg ggcattcaac cttaattgga aagaaaccca
    15901 agaagtcggt tccgttgttg aaaaagaatt gggcattcct tttgccattg acaatgatgc
    15961 taatgtggct gcccttggtg aacgttgggt aggtgctggt gaaaataacc cagatgtcgt
    16021 cttcatgaca cttggaacag gtgtcggtgg aggcattatt gctgatggta acttgattca
    16081 tggtgttgca ggagcaggtg gtgaaatcgg ccacatgatt gttgagccag aaaatggctt
    16141 tgcttgtact tgtggctcac acggctgttt ggaaacagta gcttcagcaa caggagttgt
    16201 caaagtggca cgtttattgg cagaagctta tgaaggggat tcagccatca aagcagctat
    16261 tgacaatggt gaaggtgtta ccagcaaaga tatttttatg gcagctgaag caggggattc
    16321 ctttgctgat tctgttgtgg aaaaggttgg ttactacctt ggccttgctt cagcaaatat
    16381 ttccaatata ttgaatccag actcagtggt tatcggtggg ggtgtgtcag cagcaggaga
    16441 atttctacgc tcacgtattg aaaaatactt tgtgaccttc actttcccac aagtgaggta
    16501 ttcaactaaa attaagattg cagagcttgg caatgatgct ggaattatcg gtgcagctag
    16561 ccttgctcga caatttatca caaaatagga gggggatatg tctccaatca cacttatctt
    16621 atggttactt cttgtcggca ttgttggtta ctacacatgg aactatttct cttttcgaaa
    16681 aatggcaaag caggttgata acgagacttt taaagacgtg atgcgtcaag gtcaattgat
    16741 tgatttacga gagccagcgg cttttagagc taagcatatt ttaggagctc gaaattttcc
    16801 agcgcaacaa tttgatgctg ctattaaggg actacgcaag gacaaacctg ttcttattta
    16861 cgaaaatatg cgtccccagt accgtgtgcc tgcggtcaaa aaacttaaaa aagctggctt
    16921 tgaagacgtt tatgtcttaa aagacggtat tgattattgg gatggcaaag ttaaacaaac
    16981 aacctagtta agagtaacgt ggagttaagg ggaataaagg cagtcactgt ctcaaaaacc
    17041 ttaattcctt ttttgctgta tccagacttg ctgaaagtct gaaaacattt acaattgatt
    17101 aaaaccagtt ttttaaaaca ttttgtgtta tacttatcta gttaaaatat atttacttag
    17161 aggaacaaat gactaactta agaaacgata tccgtaacgt agcgattatt gcccacgttg
    17221 accacggaaa aacaacactt gtagatgaat tattaaaaca atcccatact cttgatgagc
    17281 gtaaagagct tcaagagcgt gccatggatt ccaatgacct tgaaaaagaa cgtgggatta
    17341 cgatccttgc gaaaaatacg gcagtagcct ataacgatgt tcgtattaac atcatggata
    17401 ccccaggaca cgcggacttc ggtggtgaag ttgaacgtat catgaaaatg gttgacgggg
    17461 ttgttcttgt tgtggatgcc tacgaaggaa caatgcccca gacgcgtttc gtattgaaaa
    17521 aagcacttga gcaaaacctt atcccgatcg ttgtggtgaa caagattgac aaaccttcag
    17581 ctcgtccagc agaagttgta gatgaagtgc ttgaattatt catcgaactt ggtgccgatg
    17641 atgagcaatt ggaattccca gttgtttacg catcagctat taatggaaca tcatcattat
    17701 cagatgaccc tgctgaccaa gagcatacta tggcaccgat ctttgatacg attattgatc
    17761 atattccagc gccagttgat aattcagatg agcctttgca attccaagtg tcacttttgg
    17821 actacaacga tttcgtaggt cgtatcggta tcggtcgtgt tttccgtggt actgttaaag
    17881 tgggtgacca agtgactctt tcaaaacttg atggtaccac taaaaacttc cgtgttacaa
    17941 aactgtttgg tttcttcggt ttggaacgtc gtgaaattca agaagctaaa gcaggtgact
    18001 tgattgctgt ttcaggtatg gaagatatct ttgttggaga aaccattaca ccaactgact
    18061 gtgtggaagc tctgccaatt cttcgtattg atgagccaac gcttcagatg actttcttgg
    18121 tcaataactc tccttttgca ggtcgtgaag gtaaatggat cacgtcacgt aaggttgaag
    18181 aacgtctttt agcagaattg caaacagacg tgtcacttcg tgttgaccca acagattcgc
    18241 cagataaatg gacggtttca gggcgtggag aattgcattt gtcaattctt atcgaaacca
    18301 tgcgtcgtga aggctatgaa cttcaagtat cacgtccaga agttatcatc aaagaaattg
    18361 atggtgtcaa atgtgaaccg tttgagcgtg ttcaaattga tacaccagaa gaatatcagg
    18421 gtgcaatcat ccagtctctt tcagaacgta aaggggatat gcttgatatg cagatggttg
    18481 gtaatggtca aacgcgtttg attttcttga ttcctgcacg tggtttgatt ggttattcaa
    18541 cagagtttct ttcaatgaca cgtggatatg gtatcatgaa tcatactttt gatcagtatc
    18601 taccggttgt tcaaggagaa attggtggcc gtcatcgtgg tgccttggtt tctattgaaa
    18661 atgggaaagc aacgacatat tcaatcatgc gtattgaaga acgtggaact atctttgtca
    18721 acccgggtac agaagtttac gaagggatga ttgttggtga aaactcacgt gataacgacc
    18781 ttggtgttaa cattacaaca gctaagcaaa tgacaaatgt ccgttcagcg actaaagatc
    18841 aaactgcagt tatcaagacg cctcgtatcc taacgcttga agaatcatta gagttcttga
    18901 atgacgacga atatatggaa gtaacgccag aatctatccg cttgcgtaaa caaatcttga
    18961 ataaagctgc gcgtgataaa gctaataaaa agaaaaaatc agccgaatag cttttgaagt
    19021 taaactaact aaataaaata gcaaaaagat atttaagaaa agtcattttt taaaggtctt
    19081 tttgtgttta caagttccta tggaatataa accaaaataa aggaggacgg aatgttttac
    19141 cttattatgg ctgtcttgat tatatcttat tatctataca tggctcctaa aagtgtccgt
    19201 aacacccttg ggatgattgg tttagttggc ttggttgctc tgcttattgt tttagcgggc
    19261 ttgagtttta tcaaaatcat gcaaatccct cctgaatttt ttattgggat gggaatggtg
    19321 gcattgggct attttgcttt aaaagatgtg cgtaaaatga ccaaaaaacc gagagttaaa
    19381 taaaaatact taaagattga tgattttggt ttttatacga taaaaggact gatttgtttt
    19441 agacaatatc agtcctcttt taatagttca ttttttctga aagaaagcct ttagccgatt
    19501 ttcattgagt gtgcaactaa aaagagatgc ttggtagtga cagttaagac ctctgactag
    19561 tattttatct tattttactt aaaaaaacgt accaataatc tcacaaatat gggcatgatg
    19621 tttgtatatt ggaaacggat tctaattcta aactcaagaa cggtattttt aaaatgttga
    19681 aaaagcatct aaataaaggt aaaatagaaa tcaaatagaa tggatagtgt gagcttagat
    19741 gaaagtgata agtaattttc aaaacaaaaa aatattaata ttggggttag ccaaatcggg
    19801 cgaagcagca gcaaaattat tgactaaact tggtgcttta gtgactgtta atgatagtaa
    19861 accatttgac caaaatccag cggcacaagc cttgttggaa gaggggatta aggtcatttg
    19921 tggtagccat ccagtagaat tattagatga gaactttgag tacatggtta aaaaccctgg
    19981 gattccttat gataatccta tggttaaacg cgcccttgca aaggaaattc ccatcttgac
    20041 tgaagtagaa ttggcttatt tcgtatctga agcgcctatt atcgggatta caggatcaaa
    20101 cgggaagaca accacaacga caatgattgc cgatgttttg aatgctggcg ggcaatctgc
    20161 actcttatct ggaaacattg gttatcctgc ttcaaaagtt gttcaaaaag caattgctgg
    20221 tgatactttg gtgatggaat tgtcctcttt tcaattagtg ggagtgaatg cttttcgccc
    20281 tcatattgct gtcatcacta atttaatgcc gactcacctg gactatcatg gcagttttga
    20341 ggattatgtt gctgctaaat ggatgattca agctcagatg acagaatcag actaccttat
    20401 tttaaatgct aatcaagaga tttcagcaac tctagctaag accaccaaag caacagtgat
    20461 tcctttttca actcaaaaag tggttgatgg agcttatctg aatgatggaa tactctattt
    20521 taaagaacag gcgattatag ctgcaactga cttaggtgtc ccaggtagcc acaacattga
    20581 aaatgcccta gcaactattg cagttgccaa gttatctggt attgctgatg atattattgc
    20641 ccagtgcctt tcacattttg gaggcgttaa acatcgtttg caacgggttg gtcaaatcaa
    20701 agatattacc ttctacaatg acagtaagtc aaccaatatt ttagccacta aaaaggcttt
    20761 atcaggtttt gataacagtc gcttgatttt gattgctggc ggtctagatc gtggcaatga
    20821 atttgacgat ttggtgccag accttttagg acttaagcag atgattattt tgggagaatc
    20881 cgcagagcgt atgaagcgag ctgctaacaa agcagaggtc tcttatcttg aagctagaaa
    20941 tgtggcagaa gcaacagagc ttgcttttaa gctggcccaa acaggcgata ctatcttgct
    21001 tagcccagcc aatgctagct gggatatgta tcctaatttt gaggttcgtg gggatgaatt
    21061 tttggcaacc tttgattgtt taagaggaga tgcctaatgc ctaagaagat tttatttaca
    21121 ggtggtggaa ctgtaggtca tgtcaccttg aacctcattc tcataccaaa atttatcaag
    21181 gacggttggg aagtacatta tattggtgat aaaaatggca ttgaacatac agaaattgaa
    21241 aagtcaggcc ttgacgtgac ctttcatgct atcgcgacag gcaagcttag acgctatttt
    21301 tcatggcaaa atctagctga tgtttttaag gttgcacttg gcctcctaca gtctctcttt
    21361 attattgcca agcttcgccc tcaagccctt ttttccaaag gtggttttgt ctcagtaccg
    21421 ccagttgtgg ctgctaaatt gcttggtaaa ccagtcttta ttcatgaatc agatcggtca
    21481 atgggactag caaacaagat tgcctacaaa tttgcaacta ccatgtatac cacttttgag
    21541 caggaagacc agttgtctaa agttaaacac cttggagcgg tgacaaaggt tttcaaagat
    21601 gccaaccaaa tgcctgaatc aactcagtta gaggcggtga aagagtattt tagtagagac
    21661 ctaaaaaccc tcttgtttat tggtggttcg gcaggggcgc atgtgtttaa tcagtttatt
    21721 agtgatcatc cagaattgaa gcaacgttat aatatcatca atattacagg agaccctcac
    21781 cttaatgaat tgagttctca tctgtatcga gtagattatg ttaccgatct ctaccaacct
    21841 ttgatggcga tggctgacct tgtagtgaca agagggggct ctaatacact ttttgagcta
    21901 ctggcaatgg ctaagctaca cctcatcgtt cctcttggta aagaagctag ccgtggcgat
    21961 cagttagaaa atgccactta ttttgagaag aggggctacg ctaaacaatt acaggaacct
    22021 gatttaactt tgcataattt tgatcaggca atggctgatt tgtttgaaca tcaggctgat
    22081 tatgaggcta ctatgttggc aactaaggag attcagtcac cggacttctt ttatgacctt
    22141 ttgagagctg atattagctc cgcgattaag gagaagtaaa tggcaaaaga taaagagaaa
    22201 caaagtgatg acaagctcgt tttgacagag tggcaaaagc gtaacattga atttttaaag
    22261 aaaaagaagc agcaagctga ggaagaaaaa aaactcaaag agaaattatt gagtgataaa
    22321 aaagcgcagc agcaagctca aaatgcttct gaagcagttg agcttaaaac tgatgagaaa
    22381 actgatagtc aggaaattga gtcagaaacg acgtcaaaac ctaaaaaacc caaaaaagtt
    22441 agacaaccca aggaaaaaag cgcgacacaa atcgcttttc aaaaatcctt gcctgttctt
    22501 ttgggggcgc tcttacttat ggcggtgtct atttttatga tcactcctta tagcaaaaag
    22561 aaagagtttt ctgtaagagg aaaccatcaa acgaaccttg acgaattaat caaagctagc
    22621 aaagtcaaag catctgacta ttggttaacg ttgttaactt cgcctggtca gtatgaacga
    22681 ccgattcttc gtactattcc atgggtgaaa tctgtacatc tctcttacca atttcctaat
    22741 cactttctat ttaacgttat tgaatttgaa atcatcgctt atgcacaagt cgaaaacggt
    22801 tttcagccta ttttggagaa tggaaaacgt gtggacaagg tcagggcatc agaactaccg
    22861 aaatctttct tgattcttaa tttaaaagat gagaaagcga tccaacagtt agttaagcaa
    22921 ttaacgacat tacctaaaaa attagtcaag aatatcaagt cagtgtctct tgcaaattcc
    22981 aaaacgacag cggatttact acttattgaa atgtatgacg gtaatgtagt tagagtaccg
    23041 cagtcacaac tcacattgaa acttccctat tatcaaaaat tgaaaaaaaa ccttgaaaat
    23101 gatagtatag tggatatgga agttggaatt tatactacaa cacaggagat tgaaaatcaa
    23161 cctgaagttc ctcttacgcc agaacaaaac gcagctgata aagaaggaga taagcctggt
    23221 gaacatcagg aacagacaga caatgattca gaaacgccag caaatcagag tagtcctcag
    23281 caagcaccac catccccaga aacggtcctc gaacaggccc atggctagct aatatctaag
    23341 ttgaaaaagc aatgaaaacg ttagaaattc aacgattcta acccataatg aattgcctaa
//
LOCUS       DAUDYX010000032        22943 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.032, whole genome shotgun sequence.
ACCESSION   DAUDYX010000032 DAUDYX010000000
VERSION     DAUDYX010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22943
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.032"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..230
                     /locus_tag="VHD02_001212"
     CDS             <1..230
                     /locus_tag="VHD02_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497821.1"
                     /translation="LPIHDGAVRWLKQNMVITETTNQYMVSDLNNAVFPFMLNPWVVE
                     AMQNDEELVNEFSKAYKKMESKYNKMISNRY"
     gene            525..836
                     /locus_tag="VHD02_001213"
     CDS             525..836
                     /locus_tag="VHD02_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEQ5497822.1"
                     /translation="MDKEMININANLLKEPTFGTFTRGDEEVQVVNFALSKGYGKGRE
                     YINCAAYGNKSDVAKEFSEGDFIHVYGYFNKRTKNGKTYKNFVVMSMNKIEKKEENEE
                     E"
     gene            840..1055
                     /locus_tag="VHD02_001214"
     CDS             840..1055
                     /locus_tag="VHD02_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HEQ5497823.1"
                     /translation="MDFFVQAVNVLKILVMAVGAGLGAWGVINLMEGYGNDNPGAKSQ
                     GIKQLMAGGGIVLIGLKLIPLLSNVLK"
     gene            1068..1931
                     /locus_tag="VHD02_001215"
     CDS             1068..1931
                     /locus_tag="VHD02_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497824.1"
                     /translation="MFGIFDKIEEFFKELLLGGIQANLESMFIDINDKVGAVATDIGK
                     TPMGWNSEVFNFIKSINDSVIIPIAGLIITAVLCIELINMVMQKNNMHDTDTFEFFKY
                     MIKMWIAVWLVSHAFTFSMAVFDVAQHLVNQAAGVINTSATVSGDQIVQMVEGLKDKG
                     LGELVMILFETSLVKVAIQVMSVVIMLVVYGRMFEIYVYCSVSAIPFATMGNKEWGQI
                     GTNYIKGLFAIGLQGLFLIICLGIYAVLVKTIKITDIHASTFMILGYALLLGLMMLKS
                     GTLAKSVLNVH"
     gene            1944..2210
                     /locus_tag="VHD02_001216"
     CDS             1944..2210
                     /locus_tag="VHD02_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497825.1"
                     /translation="MIEVKKKFFPVSIIVVGIGAFTISIFNRNKLKNLEDEVKSTRDD
                     VKSILCYQEQKNAFIESELEEMRNEVASCYEHFEAFSKEKEDGR"
     gene            2214..2612
                     /locus_tag="VHD02_001217"
     CDS             2214..2612
                     /locus_tag="VHD02_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEQ5497826.1"
                     /translation="MGYVLIPKDLKKVKTKVAFNLTRRQLIGFTLAGLVGIPVYLFMR
                     KFMPNDIAILFLIVSTLPIFFVTLFEKDGLTFEKYFKHIYLHKFYQPQKRVRKEVYLE
                     QEKKNTANSIRKKSKGIKGKQKKPEKRKAD"
     gene            2515..4947
                     /locus_tag="VHD02_001218"
     CDS             2515..4947
                     /locus_tag="VHD02_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEQ5497827.1"
                     /translation="MNKKRKIQQIQLEKNQKVLKENKRSLKKEKLTKQKKKASLIDLI
                     FKKEPKRYTVEDTIPYLRMLKSGICQLDEKHYNKCIAFQDINYQLALEEDKDLIFNQF
                     ANVLNSFDPSVDIEFSYINQLGRNNELKAAIQIPDKNDGYDDIRLEFREMLKSQLAKG
                     NNGLKKSKYITFGVEADSLEQATAKLERLEIDILSNLKSMGVRAEGLTGEERLKVLHD
                     ILNPDKLFSFSYKDLKPRESTKTVIAPDSFNFVPSKYFKFGKYIGAVSHLQILASELS
                     DRMLAEFLDIDDNINISFHIKAVEQTEAIKMVKRKNTDIDKMKIEENKKAVRSGYDMD
                     ILPSDLITYGDNIKMLLKDLQTRDERMFIVTIVFMNFARTVQKLDTTISQISSIASKH
                     NCKIKRLDHSQEQGFISVLPLGVNKIEIDRGLTSSSTAVFLPFTTEELFINSSNSLYY
                     GLNALSHNLIMADRKMLKNPNGLILGTPGSGKSFSAKREMANAILTTDDDVIICDPEG
                     EYGNLVKQFKGEVIKVSAKSKDYLNPLDINMNYGDGDAPLKDKANFIMSMLELVVGGS
                     GLTAEEKSVIDRCLPRIYEKYFENPIPENMPILQDLYNMLRGQEEKVGKKLATEMEIY
                     VSGSLNVFNHQSNVDLNKQLICFDIKELGTQLKKIGMLVIQDQVWNKVSQNRNTGKST
                     RYYIDEFHLLLKDEQTSQYSVEIWKRFRKWGGIPTGITQNVKDLLASKEIENIFDNTD
                     FILMLNQATGDRDWLVVKLKISKDQEKFVTNSRAGEGLIFFGNTIVPFVDNFPKDTIL
                     YQKMTTKPEEVR"
     gene            4954..7248
                     /locus_tag="VHD02_001219"
     CDS             4954..7248
                     /locus_tag="VHD02_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ5497828.1"
                     /translation="MSKKRKRDLNEKLKAREERTVTRTETDDVLDYKRKTGDDYRDKV
                     VKEENRFQDKIHEKISKRADITSEIDTSNKSKNAIKRNLQSNSFETDSGMKPIVESPE
                     KNQRIEANSDTYRVFENTASKETHRNREVGNANDGLDSQSVNVTKRKHSKKLMSDFAE
                     TEVAKTDYQTEVKENRIYDPLAKDQDGDGVIDRYDNDFRDSDVSYEPLGNKKSKLHEK
                     QKRSLKRKNYADKLFTRKGTDKKKDDNVKASKTGKEAIKDREKKNQLYKRYNKETLVG
                     GSVIGVAKLGEVTSTYLSSGSDENASVEAAEKGLGSSSKLIHGVKNYSDKRKSKKLYD
                     FEKNDRKIQSRKSKLQFRKSMDEVKKTDQYKKANAYKKFQKKKQMKSAIYKQNKTRIR
                     DRVKKTLMDTFKVSKDFIIRKAKVVAIIMAAIIIFGTFVFNFASMTMGGFANSTSSVL
                     TTSYLSRPNVLTEINQSFSNKENDLYSELENVEKNNPGYDEYIVRKNGDIGHNVHELL
                     SYITSRCGEVKNLSEVSNILDDLFKTMYHVDYKEEIEIRYKTVTETYTDEEGNEYTES
                     HEEPYEYRKLIVTLSKREMDSVVREVFAKYPDNLKHYEALFLAQGNMGEMFGNTNLIT
                     ANGGIGGGKEYEASSDVQKKIVNAAYITPSPGAGWCAMWVSQVYQNAGLGYIGGNAND
                     MYRNHTFTSDRSKLKVGMLVAVESSSSGGQAGLTYGHVGIYIGDGKVMDNIGVVRVTT
                     LDDWIATFCKHHPVGFGFPPNVQR"
     gene            7264..7494
                     /locus_tag="VHD02_001220"
     CDS             7264..7494
                     /locus_tag="VHD02_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEQ5497829.1"
                     /translation="MNRELNTVNKKIQKLLDKKAQIDKDLEPLLIRKDELLDQEYVVI
                     CRENNISLEELVKMFKEKKEKEKYSNEKQIEE"
     gene            7472..9082
                     /locus_tag="VHD02_001221"
     CDS             7472..9082
                     /locus_tag="VHD02_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HEQ5497830.1"
                     /translation="MKSKLKNKKVLAALIGVALLLVSVICVMTENKQIVFAKDGTVLS
                     ILNPIEALAETEEKFEVKIKYVFSDEKVFKEEVVKADKGQILDSGDLPMLPDDMKFID
                     DFLYYEVKGDGKDEIIRKVEKIVKDEATQTENDKDKEKKSDEAKQDKATQTEEPKKDS
                     SAQTDITKNDLDKMDKESKDLKEKVDNLSKELKDKNKLSDKQKDKIKNLEDEIEALEK
                     KIKKDKDSIDSSKENIELKKSIEELEKKTKELQEKLNSVNKIGNNVNPTISNPVPKNS
                     GSFSSFIPSTPINTGKGSDASGNVSTPTSKTENKVETKSNKKEEKQEIRYPNKLTPKQ
                     TSSNGDTSSMDGTSKPVNTNKGVASAPSKARGSVTENKDNANKDYPIHHNDGKDNKST
                     DMYSADARQFVTFTTKNGKTFHLIINHDEDSENVMLLTEVSEDDLLNMVEKKETQKEI
                     VKEELIKEEAKPVKKEESSNMGTYLILILVVAGALGAGYYFKVVKKKENEELEALEED
                     DDDDFFSEADESDETSEIGVEEIEEDEE"
     gene            9174..10880
                     /locus_tag="VHD02_001222"
     CDS             9174..10880
                     /locus_tag="VHD02_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEQ5497831.1"
                     /translation="MDLVIAEKPSVAISIAKIIGATKKKDGYYEGNGYRVSWCVGHLI
                     QMTNPDSYDEKYAKWNMEDLPIIPSEYKYEVLKSTKKQFSILKKLLNDKEVENVVNAC
                     DAGREGESIFRLVYNQANCKKKMKRLWISSMEDSAIKDGFGNLKDGSYYDDLFESAKA
                     RAIADWLVGMNISRLYSCLYKQNYSVGRVQTPTLAMIVNRDDEINNFKKEKYYTVELS
                     LDGFSLSTDRIDDRTTTEQLINLVGNFIEIIDVVQKEKITKPELPFDLTTLQRECNKY
                     FGYSAKQTLDYAQSLYEKKLITYPRTDSRCLTEDMITSVINNILGKNDFDTERIKIVF
                     NSKKVTDHHAIIPTISSLKDNISGLPESEVKVYRLILNKLHASVGYPLVENTTKIVAE
                     FDGFEFTSSGKVIIDEGFTKYLKEYKTKKTEDTLLPNIKVGDVFEIRNKEVKEKYTTP
                     PKHFTEDTLLKAMEVAGNDALEKGIEVERKGLGTPATRAGIIENLIFKGFIERDKKNL
                     IATDKGISLVTIVSDTFKSAETTAEWEMKLSDIANGKANKDDFLKGIENEIRETVGIY
                     RK"
     gene            10936..11883
                     /gene="dcm"
                     /locus_tag="VHD02_001223"
     CDS             10936..11883
                     /gene="dcm"
                     /locus_tag="VHD02_001223"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEQ5497832.1"
                     /translation="MSELNVLELFGGIGAIKKALMRQKIPHKTVDYVELDKSCVKSYN
                     ALYDEEFEPTSIVDYHPPGEKIDLLMHGSPCQDFSRSGLKKGGTKDSGTRSSLLFETI
                     RIIEEMNIKPKVVLWENVKGVLDKNLRASFFHYLKEIERLGYESKYEILNAIDFGIPQ
                     KRERIFVVSILGNNSFDFGKLEKTQTRDISEFLEKDASNLYEVRQESMLRHIRGEPKN
                     NNFRGRLKVIDKFAYTISTKQVRIPNSGIIDLCNGKYRYLTERECFRLMGFNDEDFNK
                     LKAIYPGRKGKLSSILYKQAGNSIVVNVLEAILKEILKN"
     gene            11893..20244
                     /locus_tag="VHD02_001224"
     CDS             11893..20244
                     /locus_tag="VHD02_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEQ5497833.1"
                     /translation="MRTNDFYNIIELVKRDVLYSEREYLKLLKVIGNNQRYDFLSQLS
                     IYDKNPNATACASFDMWRERFNRTVMRGQKGIPILNDSNAFQKVGYIFDISQTVSMDR
                     NVNEVELWSFDREKHENALKDMIELQGYDSSENLPENLYSLSRIYADESIYELANNLR
                     IADEDRNSFVNFMRNSISFAISNRFNLDYPIPMDNLQENFKYLDRISLMSVGNCISNA
                     CSNIIEATMVRTKNLSVNYDLTKSIGADYNKLNENNKELGGMENAIRSNDERDDNDRN
                     RIFRDGEYGRDNFDNQREDFEQSGGREEVHGRISESDLRSDEIGLSNRERRGETLSNV
                     NRPLQREEPSETLDGSSKESHRLYEEGKAEDDGSLENRKRNESRIQGNDFSLERNGNQ
                     GNSGSLEEIIEKTNEGAENAPVFYSKEDPYNLMTDEMLERVPKLYDQEHTNLADTQVH
                     AAYIIPFRSNWTWYMTEYDRETGDAFGLVLGIEPEWGYFNIHELEELNAQRLILEDFP
                     KTFRELKDRELKKQMDEHELQMVFNGELSFEDDVIPKEVSNEVIDKFEEELSPNFAEE
                     VGSIMEEYELPKDVAISRLATSKLEEALQGTNIGIGDFNFEQLEQITSAIKEYDFYGN
                     EITEIADPKLPVWKMEHLKWLIDDWNKGLDGVTSEKIKYLKELDIELAKFNVLKGYLM
                     NDEVSISQIEEFKDNIDFVTMSEFVDSLKDYAYNNQNEKVAVKVGNEFILASKQDSLG
                     ISLEDTGRKVEVDGVEYSLNRGVDFRESTKVDTLIDSGKYEAYKIADYVVETSQVEVK
                     QETLFDYLNPEEDKEKSLDLSIGKTVYMDHEAYKIDDEVSFNEILKKNDLRLAPLRNG
                     NHMMPIVSFADDKELLEKISFDRPKLLVGDGVHYKDKDYTITRFDEMGGGLKTVTIKD
                     NVEYLGGMITGSEVIPYRLESDLERLFGIGEKKQEKVSNFRIIEDIFPDKLTPSERLN
                     NNLEAISMLNRIEKGEKDLDITAQETLAKYVGWGGLADVFDESKDGQWKEARAFLKEN
                     LSPSEYEAAKESTLTAFYTPKIVIDGVYSTLSEMGFKNGNILEPSMGVGNFIGNLPDE
                     MNESKFYGVELDSVSGRIAKLLYPESDVQVKGFEETSFSNNFFDVAIGNVPFGEFKVN
                     DREYNRNNFLIHDYFFAKSIDKVRNGGVIAFITSSGTMDKKDESIRKYINARAEFLGA
                     IRLPNDTFKGVAGTEVTSDIIFLKKRDSVLERDDDWVHLAEDENGLVYNKYFADHPEQ
                     VIGNMKEVSGRFGKTLTCEPIAFLPHEINMSSLKDRIEAAGERISKDAKYEEIELLDD
                     EVTAIPATDDVKNFSYTIIDEEVYYRENSLFVKKEVSDKNKEKIKDYLELNDALKDVI
                     YKQKEDFSEAEIKESQDKLNEVYDSFSKKHGFVNNLSNTRALREDSNFPLVSSIEILD
                     EEENFKAKGDIFSKRTITKAKVIDHVDSSLEALVLSISQKGYVDFDYMTNLTDKDRNT
                     LIEELRGEIFLNIREENVSFNQKLSFDLEDGDLPFACSDETNSFKYTYVTKDEYLSGN
                     IREKIGIVDSYINRLRQAKRMLPEESENERETLVNELSRLEYQKAELQRVMPKELEAS
                     EINVRLGATWIPPKDIERFVFETLKTPGYARWDIKVKFSHLTSEWNVEGKSKDRGNDL
                     AEMTYGTSRVSAYKLIEDALNLKETKVFDQILNPDGSKTSVLNKKETMLAGQKQELIK
                     EEFKNWIFSDQDRRSRLVKAYNEKFNSIRNREYDGSNLSFDGMSTDISLRSHQKNAIA
                     RILYGGNSLLAHVVGAGKTFEMVASSMEAKKLGMCTKSLFVVPNHLTGQIGREFMQLY
                     PSANIMVADKKDFEPKNRKRFIGKIATGEYDAVIIGHSQFEKIPMSKEYQQKHIQDQI
                     DEIINYVEEYKHDRNQNFTVKELQKTRKKLETRLEKLNDDFKKDDVITFEELGVDKLI
                     VDEAHNYKNLYLYTKMRNVAGIGQSEAFKSSDMFMKCRYLDEMTGGKGIVFATGTPVS
                     NSMTELYTMQRYLQYDTLKKNGLEHFDSWASTFGETQSAFELSPEGTGYRVKTRFSKF
                     YNLPELMSMFKEVADIQTADMLNLPVPEAHFKVIKTEPSEEQKEILKSLSERADKVRN
                     KSVEPEEDNMLKITNDGKKLALDQRLINPLLPDDENSKVNVCVKNVFSIWDKTKEEKS
                     TQLLFSDMSTPKGDGSFNIYDDIRDKLVDLGIPKEEIAFIHESNTDKQKDELFAKVRK
                     GEIRILMGSTQKMGAGTNVQNKLIAMHDLDVPWRPADLEQRSGRIVRQGNENKEVNIY
                     RYITENTFDAYLWQTIENKQKFISQIMTSKTPVRVAEDVDESSLNYAEIKALATGDPK
                     IKEKMDLDNEVTKLKMLEANYKSNRYRLEDKVAKTYPEEIARTEKLIEAVKKDIENVE
                     PQGSGENKFTSITINGEIIRDKKIAREKLLEAIKGVKINESKVIGQYRNMDLEVSYNF
                     FTNEHNFTLKGATNHLGELGTSADGNITRLDNAIDKMPEKLNRLEEKLVSTKEQLENA
                     KEELTKPFEKADELKTKVLRLAELNKLLDMGEVEEKENPNPLLEDVKRAIVDFCKREY
                     EDDSYTYENFNNLFPDLAHIGIAYTTTPDEKHEIQYEISLEDFTATQYINGEPITKID
                     YFKDLGSEEKALEFLKQEMEYGDFGEFVSIDDNDLKKALGLERDEDGNIYDSLAKDLD
                     NDGVADRYDNDFRDSDYFETTYDVEDNIHTRAESKEKVSEKPSILGQIKSYQSEEKQA
                     ETRENKSKEHEER"
     gene            20298..20957
                     /locus_tag="VHD02_001225"
     CDS             20298..20957
                     /locus_tag="VHD02_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEQ5497834.1"
                     /translation="MNYKEMRNALEQMANENHEDFAKALISFEKGINDKDALDKLYQE
                     YMDNDSMSLLNDEFDYLIDELRENGQIKDSAAIEKEDNNLVNIVGNIVGEVETIEREN
                     KNGETFKVINFSVVSKDDEGNKIYTNCSAYGDKGDIPKNFKQGDFVKLFGQVRSSIDD
                     NGKEHTNVRILSSKLLKAKEQMKNQEEKKESVLGAIKKYKAEEKAKPVEKKKSSKETE
                     R"
     gene            21105..21335
                     /locus_tag="VHD02_001226"
     CDS             21105..21335
                     /locus_tag="VHD02_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ5497835.1"
                     /translation="MSKLSYKKLFKKLIDIEMKNTELMDKAKVSKSTFYKIKNGENVT
                     TDVLLRICDVLECDISEIVECVSIENGGKTGV"
     gene            21313..22398
                     /locus_tag="VHD02_001227"
     CDS             21313..22398
                     /locus_tag="VHD02_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010680157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEQ5497836.1"
                     /translation="MEEKRVSKFTVIDLFSGAGGLSKGFLDAGFDVLLGVDNDKAALS
                     TFELNHNGAKALSLDLSDKKVFKDIENTIGNRELDVIVAGPPCQGFSLSGPRNFDDER
                     NKLYLAVIKTVKTFKPKAFIIENVPGMATLYNGEIKNEILKRFKKMGYNISCKILCAA
                     DYGVPQMRKRLIFMGVRKDVGEPHFPDPIYDEKNYITCRDAISDLPSRESELGENTDI
                     YNDFPKTKYQIKMRGKCKVLSNHVGTNHTTLVKETIALVPEGGNYKDLPEGWGESRKF
                     NEAWTRYDGNKPSKTIDTGHRNHFHYKYNRVPTVRENARLQSFPDDFVFLGTKTQQYR
                     QVGNAVPPLLGFALANELIKILEKEFK"
     gene            22398..>22943
                     /locus_tag="VHD02_001228"
     CDS             22398..>22943
                     /locus_tag="VHD02_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010680158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEQ5497837.1"
                     /translation="MSKNTNKIKSIDLFAGCGGLMDGFEQSGFYKTLAAVEWEKAPCD
                     NLKIRLKDKWKYHNSEEIVMQFDIQRTDELFEGWNEEKYGASKGLDYIVNKNSGIDII
                     IGGPPCQAYSIAGRVRDENGMKDDYRNYLFESYLKVVKKYSPKLFVFENVPGLLSAKP
                     GDRNIVDIIKEQFEEAGYSILE"
BASE COUNT         8850 a         2874 c         4822 g         6397 t
ORIGIN      
        1 aattacctat acacgatggg gctgtaagat ggcttaaaca aaatatggtg ataacagaaa
       61 caacaaatca gtacatggtt tctgatttaa ataatgcagt ttttccattt atgttaaatc
      121 cctgggtagt agaagctatg caaaatgacg aagagctagt aaatgaattt tcaaaagcat
      181 ataaaaaaat ggaaagcaaa tacaataaaa tgatctccaa tagatattaa aataattgca
      241 ataaagcgac gatagaaaga aaaaatctat cgtctttttt aatccctatt ttcaatttat
      301 attgcgatca ataaatagga aaaactcgaa gagaagctaa ctagtatttc aaatgcctct
      361 cactaatata tacacgacaa aatgctagtt ccactaaata tgttacagaa agtaaaggag
      421 aaaagatgat aaggataaga agccctacct aatagacaat taaatataca aatacttggc
      481 gattgaaatt aacttttcag tcgctttttt aattggagga aaaaatggat aaagaaatga
      541 ttaatatcaa tgccaacctg ttaaaagaac ctacttttgg aacttttaca agaggtgatg
      601 aagaggttca agttgtaaat ttcgctcttt cgaaaggata tggaaaaggt agagagtata
      661 tcaactgtgc agcctatggc aataaatctg acgtggctaa ggaattttca gaaggtgatt
      721 tcattcatgt gtatggttac ttcaacaaac gaactaaaaa tggaaagaca tataagaatt
      781 ttgtagttat gtctatgaac aaaattgaaa agaaagaaga aaacgaggag gaataaataa
      841 tggatttttt cgtacaagca gttaatgtat taaagatttt ggtaatggca gtaggtgctg
      901 gtcttggagc atggggtgtt atcaacctta tggagggata tggtaatgat aaccctggag
      961 caaaatctca aggtattaaa cagctaatgg ctggtggagg catcgttcta atcggcttaa
     1021 aactaatccc attactttca aatgtattga agtaaggaga gtagcctatg tttggtattt
     1081 tcgataagat agaggaattt tttaaagagc ttcttctcgg aggtatccaa gcaaatcttg
     1141 agtcaatgtt tatcgacatt aatgataaag tcggagcagt agcaaccgat ataggcaaga
     1201 caccaatggg atggaactca gaggtattta actttattaa aagtatcaat gatagtgtca
     1261 ttattccaat tgcaggttta atcattacag cagttttatg tattgaactg ataaatatgg
     1321 ttatgcaaaa gaataatatg cacgatacag atacctttga gttttttaaa tacatgataa
     1381 agatgtggat agcggtatgg ctagtatctc atgcctttac attctcaatg gcagtctttg
     1441 atgttgcaca acaccttgta aatcaggcgg caggagtaat caatacttct gctaccgttt
     1501 caggtgatca aattgttcag atggttgagg gattaaaaga caaaggactt ggagaactgg
     1561 tgatgatact ctttgaaacc tcgcttgtta aagttgcaat acaggttatg tcggtagtca
     1621 taatgctagt tgtctatggc agaatgtttg agatatatgt ttactgttca gtttcagcaa
     1681 ttccatttgc cactatggga aataaggagt ggggacaaat tggaacaaat tatatcaaag
     1741 gtctgtttgc tattgggctt caaggcttat tcttgattat atgcttgggt atttacgcag
     1801 ttttagtaaa gacgataaag ataacggaca ttcatgcaag tacctttatg atattaggct
     1861 atgcattact tcttggtcta atgatgttaa agagtggaac gcttgcaaag agtgtattaa
     1921 acgtacatta aggaggattt ttaatgatag aagtaaagaa aaagtttttt cctgtttcaa
     1981 ttattgtagt agggattgga gcatttacta tctcaatttt taacaggaat aaattaaaga
     2041 atttagaaga tgaagtaaaa agtacaagag atgatgtgaa atctattctt tgttatcaag
     2101 agcagaaaaa tgcattcatt gagtcagaat tagaagagat gagaaatgaa gttgcttcat
     2161 gctatgaaca ttttgaagca ttttcaaagg aaaaagaaga tgggaggtaa gctatgggct
     2221 atgtactaat tcccaaagat ttaaaaaagg taaaaacaaa ggtagccttt aaccttacaa
     2281 gaagacaatt aataggattt acacttgctg gactggttgg tataccagtc tatttattta
     2341 tgagaaagtt tatgccaaat gatatagcga tactgttttt aattgtatca actcttccaa
     2401 tcttctttgt aacactattt gaaaaagatg gacttacctt tgagaaatat tttaagcaca
     2461 tatatctaca caagttttat caaccacaga agcgagtgag aaaggaggtt taccttgaac
     2521 aagaaaagaa aaatacagca aattcaatta gaaaaaaatc aaaaggtatt aaaggaaaac
     2581 aaaagaagcc tgaaaaaaga aaagctgact aagcagaaga aaaaagcttc tttgattgac
     2641 ttaatattta agaaagagcc taagagatat actgttgaag acacaatacc ttatctgaga
     2701 atgctaaaaa gtggaatatg tcagcttgat gaaaagcact ataataagtg tattgcattt
     2761 caggatataa actatcagct tgccttagaa gaagataagg atttgatttt caatcaattt
     2821 gcgaatgtct taaactcttt tgatccaagt gtagatattg agttttcata tatcaatcag
     2881 cttggaagaa ataatgaatt aaaagctgct attcagatac cggataaaaa tgatggttat
     2941 gatgatattc gattagagtt tcgtgagatg ttaaaaagtc agcttgcaaa gggaaacaac
     3001 gggctaaaga aatcgaagta tatcaccttt ggagtggaag cggatagttt ggagcaagcg
     3061 actgcaaaac ttgagcgact tgagattgat atattatcaa atctaaagag catgggagtt
     3121 cgtgctgagg gcttaactgg agaagaaaga ttaaaggtat tgcatgatat tttaaatcca
     3181 gataagctct tttctttttc ctataaagac ttaaaaccaa gagaaagtac aaagactgtt
     3241 attgcaccag attcttttaa cttcgtgcca agtaagtatt ttaagtttgg aaaatacatt
     3301 ggagctgtaa gtcatcttca aatacttgca agtgagctat cggatagaat gcttgcagaa
     3361 tttttagata tagatgataa catcaatatt tcttttcata tcaaggcagt tgagcagaca
     3421 gaagctatta aaatggtgaa aagaaaaaat acagatattg ataagatgaa gattgaggaa
     3481 aataaaaagg cagttagaag tggctatgac atggatatct taccaagtga tttgattacc
     3541 tatggagata atatcaagat gcttttaaaa gacctccaga caagagatga gcgtatgttt
     3601 atcgtaacga ttgtctttat gaattttgca aggacagtac aaaagctaga tacgactatt
     3661 tctcagattt catctattgc aagtaagcat aattgcaaga ttaaacgctt agatcatagc
     3721 caagagcaag gttttatcag tgtattacct cttggcgtaa ataagattga aatagataga
     3781 gggttaacct catcatctac agcagtcttt ttaccattta caacagagga gcttttcata
     3841 aactcgtcaa atagcttgta ctatggatta aatgccttaa gccataatct tattatggct
     3901 gatagaaaaa tgcttaaaaa tccaaatggc ttaattttag gtacgcctgg ttctggtaag
     3961 tcattttctg caaagcgtga aatggcaaat gctattctta caaccgatga tgatgtcatt
     4021 atctgtgatc cagagggtga atatggcaat cttgttaaac agttcaaggg ggaagtcata
     4081 aaggttagtg caaagagtaa ggactatctc aatccactgg atataaatat gaattatgga
     4141 gatggtgatg caccacttaa ggataaggca aattttatta tgtctatgct tgaacttgtg
     4201 gttggtggta gtggacttac ggcagaagaa aagtctgtta tagataggtg tttacctaga
     4261 atttatgaaa agtattttga aaatccaatt ccagagaata tgcctatact tcaagacctt
     4321 tacaatatgt taagagggca agaagaaaag gtgggaaaga aacttgcaac agaaatggaa
     4381 atctatgttt caggaagtct taatgtcttc aatcatcagt caaatgtaga tttaaataaa
     4441 cagcttattt gctttgatat taaagaactt ggaacgcagc ttaagaagat aggtatgctt
     4501 gttattcaag accaggtttg gaataaggtt tctcagaata gaaataccgg caaaagtaca
     4561 aggtattaca ttgacgagtt tcacttgctt ttaaaggatg aacagacttc acagtattcg
     4621 gtggaaatct ggaagagatt tagaaaatgg ggaggtattc caacaggaat tacacaaaat
     4681 gtcaaagatc tacttgcaag taaagaaatt gaaaatattt tcgacaatac ggattttatt
     4741 ctaatgctta atcaagcaac aggtgataga gattggcttg ttgtaaagct taaaatctca
     4801 aaggatcagg aaaaattcgt taccaattca agagcaggtg aaggtttgat tttctttgga
     4861 aacaccattg ttccatttgt agataacttc cctaaagaca caatcctata tcaaaagatg
     4921 actacaaaac ctgaagaagt gaggtagcgt cttatgagta aaaaaagaaa gcgagatttg
     4981 aatgagaagt taaaggcaag agaagaaaga accgtcacaa gaactgaaac agatgatgtc
     5041 ctagattaca aaagaaaaac tggagatgat tacagagata aggttgttaa agaagaaaac
     5101 aggtttcaag ataagattca cgagaagata agtaaaaggg ctgatattac tagtgaaata
     5161 gacacaagta acaagagcaa aaatgctata aaaagaaatc ttcaaagtaa cagttttgaa
     5221 actgatagtg gaatgaagcc tattgtagaa tctccagaga aaaaccaaag gatagaagct
     5281 aattcagata cttatagagt ttttgaaaat actgcttcta aagaaactca taggaataga
     5341 gaagtaggta atgctaatga tggtttggat agtcaaagcg taaatgttac taagcgaaaa
     5401 cattctaaaa aattgatgag tgattttgct gaaacagaag tagctaagac agattatcaa
     5461 acagaggtta aagaaaatcg tatctatgat cctttggcaa aagaccaaga tggtgatggt
     5521 gtcattgata gatatgataa tgactttaga gatagtgatg tatcctatga gcctttaggt
     5581 aataaaaagt ctaaacttca tgaaaagcag aaaagatcac ttaaaagaaa gaactatgct
     5641 gataagctct ttacaagaaa agggacagat aagaaaaaag atgataatgt taaagcctct
     5701 aaaacaggta aagaagctat taaagatagg gaaaagaaaa atcagcttta taaaaggtat
     5761 aacaaagaaa ccttagtcgg tggaagtgtg attggagtcg caaaacttgg tgaagttaca
     5821 agtacttatc tatcatctgg tagtgatgaa aatgcatcag ttgaagctgc cgaaaaaggt
     5881 ttagggagtt catctaaact tattcatggc gtaaagaatt attcggacaa gagaaaaagt
     5941 aagaagctat atgattttga aaagaacgat aggaaaatcc aaagtagaaa atctaagctt
     6001 cagtttagaa aaagcatgga cgaggttaag aagaccgacc aatataaaaa agcaaatgct
     6061 tataaaaagt ttcaaaagaa gaaacagatg aagtcagcta tctataaaca aaataagaca
     6121 agaattagag atagagttaa aaagacactg atggatacat ttaaggtatc taaagacttc
     6181 atcataagaa aagcaaaagt ggtagcaatc ataatggccg ccataattat ttttggaact
     6241 tttgtattta actttgcaag catgacaatg ggtggatttg ctaactctac aagtagtgtt
     6301 ttaacaacat catatttatc aagaccaaat gttttaactg agattaacca aagtttttca
     6361 aacaaggaga atgacttata tagcgagctt gaaaatgtag aaaagaataa tcccggatac
     6421 gatgaatata tcgtaagaaa aaatggagat attggtcata atgttcatga acttttatcc
     6481 tatatcactt caagatgtgg tgaagtaaag aatttatctg aagttagtaa tattttagat
     6541 gacttattta agactatgta tcatgtcgat tataaagagg aaatagagat aaggtataag
     6601 actgtaactg aaacttacac cgatgaagag ggaaatgaat atactgagag ccacgaagaa
     6661 ccttatgagt atagaaaact cattgtaact ttaagtaaaa gggaaatgga tagcgtggtt
     6721 cgagaagtct tcgccaagta tccagataat ttaaagcatt atgaagcact attccttgca
     6781 caggggaata tgggtgaaat gtttggaaac acgaatctca ttactgcaaa tggtggcatt
     6841 ggtggaggaa aagaatacga agcatcgtct gatgttcaaa agaaaattgt aaatgcagca
     6901 tatattacac cgtcacctgg agcgggctgg tgtgcaatgt gggtttcaca ggtttatcaa
     6961 aatgcaggtc ttggatatat cggaggaaat gctaatgata tgtaccgaaa tcatacattt
     7021 acttctgata gatcaaagtt aaaagttgga atgcttgtag ctgttgaaag tagcagtagt
     7081 ggtggacaag ccggacttac ttatggtcat gtcggtatct atattggtga tggtaaggta
     7141 atggataata ttggagtagt cagggttacg actttagatg attggattgc aacgttttgt
     7201 aaacatcatc cagtaggctt tggatttcca ccaaatgtac aaagatagaa aggagcggag
     7261 aatgtgaata gggaactgaa tactgttaat aaaaaaattc aaaagttatt agataagaag
     7321 gctcagatcg ataaagattt agagccttta ttaattcgta aagatgaact attagatcag
     7381 gagtatgtag tgatttgtag agagaataat atctctcttg aggaattagt gaagatgttt
     7441 aaagagaaaa aagaaaagga gaaatatagt aatgaaaagc aaattgaaga ataagaaagt
     7501 attggccgca cttattggag tagcattact tttggtttca gttatatgtg tgatgactga
     7561 aaataagcag attgtatttg caaaagatgg aactgtactt tcaattctta atccgattga
     7621 agctcttgca gaaacggaag agaaatttga agtaaagatt aagtatgtat tttcagacga
     7681 gaaagtattt aaggaagagg ttgtaaaggc agataaagga caaatccttg atagtggtga
     7741 cttgccaatg ttacctgacg atatgaaatt cattgatgat tttctttatt atgaagtaaa
     7801 aggcgatggg aaagatgaga ttatccgtaa ggtagaaaag attgtaaaag atgaagcaac
     7861 tcagactgaa aatgataaag ataaagaaaa gaagtcagat gaagcaaaac aagataaagc
     7921 cactcaaaca gaagaaccta agaaagattc atcagctcaa actgatatta ctaaaaatga
     7981 tttagataag atggataagg agtctaagga cttaaaagaa aaggtagata atcttagcaa
     8041 ggaacttaag gacaaaaaca agctaagtga taagcaaaaa gataaaatca aaaatcttga
     8101 agatgaaatt gaagctttgg aaaagaaaat caaaaaggat aaggattcta ttgattcatc
     8161 aaaggaaaat attgagctaa aaaaatctat tgaagagtta gaaaagaaga ctaaggaact
     8221 acaggaaaag cttaattctg taaataagat agggaataat gttaatccaa ctatttcaaa
     8281 tccagtacct aaaaacagtg gatcatttag tagttttatt ccaagcacgc ctataaatac
     8341 aggaaaaggt agcgatgctt ctggtaatgt ttcaacgcca acatctaaga cagaaaataa
     8401 ggtggaaact aagtcaaata aaaaagagga gaaacaggaa atccgttatc cgaataagct
     8461 aacacctaaa cagacttcaa gtaatggaga tacgtcttct atggatggaa caagcaagcc
     8521 tgtaaataca aataaaggag tagcgtcagc accatcaaag gcaagaggat ctgttacaga
     8581 gaataaggat aatgcaaaca aagattatcc aattcaccac aacgatggta aagataataa
     8641 gtctacggat atgtattcag cagacgcaag acagtttgta acttttacaa ctaaaaatgg
     8701 aaagactttt catctaatca ttaaccatga tgaagatagc gaaaatgtaa tgcttttaac
     8761 agaagtttcg gaagacgact tattgaacat ggttgaaaag aaagaaacac aaaaggagat
     8821 tgtaaaggaa gaactaataa aagaagaggc taaaccagtg aaaaaagaag agagtagtaa
     8881 tatgggaaca tacttaattc tgattcttgt agtagctgga gcattaggtg caggttatta
     8941 ctttaaggtt gttaaaaaga aagagaatga agaacttgaa gcattagaag aagatgatga
     9001 cgatgatttt ttctcagaag ctgatgaaag tgatgaaaca tcagaaatag gggttgaaga
     9061 aatcgaagaa gatgaagaat aaaatgcacc ataaactata tgattttggg tgcaagttaa
     9121 aggcgagaga gtattatctt tcgccttatt ttatttgaaa gattggagat aatatggact
     9181 tagttattgc agaaaaaccg agtgtagcta tatcaattgc aaagataata ggagctacaa
     9241 aaaagaaaga tggatactac gagggaaatg gatatagggt atcctggtgc gtgggtcatc
     9301 tgattcagat gacaaatcct gatagctacg atgaaaagta tgctaagtgg aatatggaag
     9361 atttaccgat tattccgagt gaatataagt atgaggtttt aaaatccact aagaaacaat
     9421 ttagtatctt gaagaaacta ttgaatgata aagaagtaga gaatgtagta aatgcttgtg
     9481 atgcaggtcg tgagggggaa agtattttta gacttgttta caatcaagca aattgtaaaa
     9541 agaagatgaa acgactttgg atttcatcaa tggaagatag tgcaataaaa gatggttttg
     9601 gaaacttaaa agatggaagt tactacgatg atttatttga atctgccaag gcaagagcga
     9661 ttgctgactg gctagtcgga atgaatatta gtaggcttta ttcttgcttg tataagcaaa
     9721 attatagtgt gggaagagta caaacaccta cacttgcaat gattgtaaat agagatgatg
     9781 aaattaataa tttcaagaaa gaaaaatact atacagtaga gctttcttta gatggatttt
     9841 cgctttctac agatagaatt gatgacagga ctacaacaga gcagctaatc aatttagtag
     9901 gtaatttcat agaaattatt gatgtcgttc aaaaagaaaa gataacaaag cctgagttac
     9961 catttgacct cacaacactt caaagggaat gtaacaagta ttttggatat agtgcgaaac
    10021 agacacttga ttatgctcaa agtctttatg agaagaaact tatcacatac ccaagaactg
    10081 acagcagatg cctgactgaa gatatgatta caagcgtcat taataatatc ttaggtaaga
    10141 atgactttga tactgaaaga attaagatag tgtttaactc caaaaaagtt acggatcacc
    10201 atgcgattat accgacaatc agctcattaa aagataatat ttcaggtctt ccagaaagtg
    10261 aagtaaaagt atataggctt atattaaata aacttcacgc aagtgttgga taccctttag
    10321 tagaaaatac tacaaaaatt gttgctgagt ttgatggctt tgaatttaca agtagtggca
    10381 aagtgataat tgatgaggga ttcactaagt atctaaaaga atataaaacg aagaaaacgg
    10441 aagatacatt actcccgaat ataaaggttg gagatgtatt tgaaattaga aataaagaag
    10501 taaaagagaa atatacaaca ccacctaaac attttacgga agatacactt ttaaaggcta
    10561 tggaagtagc aggtaatgac gcattagaaa aaggcattga ggtagaaaga aaaggacttg
    10621 gtacaccggc aactcgtgca ggtattattg aaaacttgat cttcaaaggt tttatcgaaa
    10681 gagataaaaa gaatttaatt gcaaccgata aaggaattag tttggtgact attgtttcag
    10741 atacttttaa atcagcagaa actactgccg aatgggaaat gaaactatct gacattgcta
    10801 atggcaaggc gaataaggat gacttcttaa aaggtataga gaatgaaata agagaaacag
    10861 taggaatata tcgtaaataa tttttagggg aatgagagct aatcttgttc ccttatttta
    10921 ttggaggtga tgaaaataag tgagttgaat gtattagaac tgtttggtgg tataggtgct
    10981 attaaaaaag ctttaatgag gcaaaaaata cctcacaaga cggttgatta tgtagagcta
    11041 gataagagct gtgttaaaag ctataatgca ctttatgatg aagaatttga gcctacaagt
    11101 atagtagatt accatccacc aggtgaaaaa atagatttat taatgcatgg tagtccttgt
    11161 caagatttca gtagaagtgg cttaaagaaa ggtggcacaa aggacagtgg tacaagaagt
    11221 agtcttcttt ttgaaacaat acgaataata gaagaaatga atatcaagcc caaggttgtg
    11281 ctttgggaaa atgtaaaggg agttctcgat aaaaatttga gagcttcctt ttttcattac
    11341 ttaaaagaaa ttgagagact aggatatgag agcaaatatg aaattctaaa tgctatagac
    11401 tttggaatac cacagaaaag ggagcgtata tttgttgtaa gcatattggg gaataattct
    11461 tttgattttg gcaaactcga aaaaacacaa acaagggata tatctgaatt tcttgagaaa
    11521 gacgcttcaa atctttatga agtaaggcaa gagtcaatgc ttagacatat aagaggagaa
    11581 cctaagaata ataattttag aggaagactt aaagtgatag ataagtttgc ctacacaatt
    11641 tccaccaagc aagtgcgtat tcctaattca ggaattattg atttatgtaa tggcaaatat
    11701 agatatttaa ctgagcgaga atgctttaga cttatgggtt ttaatgatga agactttaat
    11761 aaactcaaag ctatatatcc aggaagaaaa ggtaaattat cgtcaatact gtataaacaa
    11821 gctggaaata gcattgtcgt aaatgtgtta gaagcaatat taaaagaaat tttgaagaac
    11881 taggaggtaa agatgagaac gaatgatttt tacaacataa ttgagcttgt aaaaagagat
    11941 gttttatata gtgaaagaga atatctaaag ctcttaaaag tcattggaaa taaccaaaga
    12001 tacgactttt taagtcaact tagtatctat gataaaaatc ccaatgcaac tgcttgtgca
    12061 agctttgata tgtggagaga aagatttaat cgaactgtta tgagagggca aaaagggata
    12121 cctattctaa atgacagtaa tgctttccaa aaggtaggat atatcttcga tattagtcag
    12181 acggtatcga tggatagaaa tgttaatgaa gtggaactat ggtcttttga tagagaaaag
    12241 catgaaaatg cattaaaaga tatgattgag ctgcaagggt atgattcaag tgaaaaccta
    12301 ccagagaatc tatattctct tagcagaata tacgcagatg aaagcatata tgagcttgcg
    12361 aataatctga ggattgctga tgaagataga aactcatttg taaattttat gagaaattct
    12421 attagctttg caatttcaaa tagatttaat ctggactatc ctattccaat ggataactta
    12481 caagaaaatt ttaagtacct agatagaatt tctttaatga gtgttggtaa ctgtatttcc
    12541 aatgcttgta gcaatattat tgaagctacg atggttagaa caaaaaattt atctgtcaat
    12601 tacgacctga caaagagtat aggtgccgat tataataagc ttaacgaaaa taataaagaa
    12661 ttaggaggta tggagaatgc tattcgatca aatgacgaaa gagatgataa tgacagaaac
    12721 cgaattttca gggatggaga atacggacga gataattttg ataatcaaag agaagacttt
    12781 gaacaatctg gaggaagaga agaagttcat ggaagaattt ccgaatccga tttacgcagt
    12841 gatgagattg gactttctaa cagagaacgg cgaggagaaa cactatcaaa tgttaataga
    12901 cctttacaaa gagaagagcc tagtgaaaca cttgatggaa gttcaaaaga gagccatcga
    12961 ctttatgagg aaggaaaagc cgaagatgat ggaagcttgg aaaatagaaa gcgaaacgag
    13021 tccagaatac aaggtaatga tttcagcctt gaaagaaatg gcaatcaagg aaatagtgga
    13081 agcttagaag aaatcattga gaaaacgaac gagggagcag aaaatgctcc cgttttctat
    13141 tctaaagaag atccatataa tttaatgact gatgaaatgc ttgagcgtgt tccaaagctt
    13201 tatgaccaag aacatactaa tctagcagat actcaggttc atgcagcata tatcattcca
    13261 tttagaagca attggacatg gtatatgacg gaatatgata gggaaacagg agatgcattt
    13321 ggtcttgttt taggaattga accagagtgg ggatatttta atattcatga gcttgaagaa
    13381 ctaaatgctc aaagacttat tttagaagat ttcccaaaga cttttagaga acttaaagac
    13441 agagagttaa aaaagcagat ggatgagcat gagcttcaaa tggtctttaa tggggaactc
    13501 agttttgaag atgatgtaat tcctaaagaa gttagcaatg aagtaataga taaatttgaa
    13561 gaagaattaa gtcctaattt tgctgaagaa gttggctcta ttatggaaga atacgaacta
    13621 ccaaaagatg ttgcaattag tagacttgct acaagtaagt tagaagaagc acttcaaggt
    13681 acaaacattg gaattggtga ttttaatttt gagcagttag aacagattac gagtgccata
    13741 aaggagtatg acttttatgg aaatgaaatt actgagattg cagatcctaa gcttcctgta
    13801 tggaagatgg aacatttaaa atggcttatt gatgattgga ataaaggatt ggatggtgtt
    13861 acatcagaaa agataaagta tctgaaagag ttagatattg aacttgctaa gtttaatgtc
    13921 cttaaaggtt atctaatgaa tgatgaagta agcataagcc agattgaaga atttaaggat
    13981 aacattgatt ttgttacgat gagcgagttt gttgatagcc ttaaagatta cgcatataac
    14041 aatcaaaacg agaaagtcgc agttaaagtc ggcaatgaat ttatacttgc aagtaaacaa
    14101 gatagcttag gaattagcct tgaagatact ggaagaaaag tagaagttga tggagtggaa
    14161 tattcactta atcgaggagt agattttagg gaaagcacaa aggttgatac tttaattgat
    14221 agtggaaaat atgaggcata taagattgct gattatgttg tagaaactag tcaagttgaa
    14281 gtaaaacaag agacactttt tgattacctt aatccagaag aagataaaga aaaatcatta
    14341 gacttatcta ttggaaaaac ggtctacatg gatcatgagg catataagat tgatgatgaa
    14401 gtgagcttta atgagattct aaaaaagaat gatttaagac ttgctcctct aagaaatggt
    14461 aatcatatga tgcctatagt aagttttgca gatgataaag agttattaga aaagattagc
    14521 tttgatagac ctaaactact tgttggagac ggagttcact ataaagataa agactacact
    14581 ataactcgct ttgacgagat gggtggtggt cttaagactg ttacaataaa agataatgtt
    14641 gagtatcttg ggggaatgat tacgggttca gaagttattc cttatagatt agaaagtgac
    14701 ttagaaagac tttttggaat tggggaaaag aaacaagaga aagtatcaaa ctttagaatt
    14761 atagaagata ttttccctga taaacttact ccaagtgaaa ggttaaataa taaccttgaa
    14821 gccatttcaa tgcttaacag gatagaaaaa ggtgaaaagg atttagatat tacagcacaa
    14881 gaaactttag ctaaatatgt tgggtggggt ggactggctg atgtctttga tgaaagtaaa
    14941 gatggacaat ggaaagaagc tagagctttc ttaaaagaaa atttatcacc atcagaatat
    15001 gaggctgcta aagaatctac cttaacagct ttttatacgc ctaaaattgt aattgatgga
    15061 gtatatagta cgctttcaga aatgggattt aagaatggaa atattttaga accatcgatg
    15121 ggcgtaggta attttatcgg taatcttcca gatgaaatga atgagtcaaa gttttatgga
    15181 gttgagcttg attctgttag tggtagaatt gcaaaacttc tttatcctga aagtgatgtg
    15241 caagttaaag gctttgaaga gacaagtttt tcaaataact tctttgatgt ggcaatcggc
    15301 aatgttccgt ttggtgaatt taaggttaat gatagggaat acaatcgtaa taacttcctt
    15361 attcatgact atttctttgc taaatccatt gataaggtga gaaatggtgg tgttatagcc
    15421 ttcatcacat catctggaac aatggacaag aaagacgaaa gcatccgtaa atatataaat
    15481 gcaagagcag agtttttagg tgcaataaga cttcctaacg atacctttaa gggtgttgct
    15541 ggaactgagg tgacaagcga tattattttc ctgaagaaaa gagatagtgt acttgagcgt
    15601 gacgatgact gggttcattt agcagaagat gaaaatggtc ttgtatataa taaatatttt
    15661 gcagaccatc cagaacaagt tattggaaac atgaaagagg taagtggaag atttggaaaa
    15721 actcttacat gtgagccaat agcattttta cctcatgaaa taaatatgtc ttctttaaaa
    15781 gatcgtattg aagctgcagg tgaaagaata tcaaaagatg cgaagtatga agagatagag
    15841 ttattagatg atgaagtaac agcaatccca gcaacagatg atgtaaaaaa cttttcctac
    15901 acaatcatag atgaggaggt atattacagg gaaaattcac tttttgttaa gaaagaagta
    15961 tcggacaaaa acaaggaaaa gattaaggat tatctcgaat taaatgacgc attaaaggat
    16021 gtaatataca agcaaaaaga agactttagt gaagctgaaa tcaaagaatc tcaggataaa
    16081 ttaaacgaag tctatgacag tttttctaag aagcacggct ttgtaaataa cctgagtaat
    16141 actagagcct tacgagaaga tagtaacttc ccactggtat cttctattga gatattggat
    16201 gaagaagaaa actttaaggc aaagggagat atattctcga aaagaaccat tacaaaagcg
    16261 aaagttattg accatgtaga ttcttcactc gaagcattgg tattatctat ttcacaaaaa
    16321 ggctatgtag attttgatta catgacaaat ctaaccgata aggatagaaa tactcttata
    16381 gaagaactta gaggagaaat ctttctaaat attagggagg agaatgttag ttttaatcag
    16441 aagctttctt ttgacttgga agatggtgat ttaccatttg cttgtagcga tgagactaac
    16501 tcctttaagt atacctatgt aacgaaagat gaatacttaa gtggaaatat tagagaaaaa
    16561 ataggtattg tagatagtta tatcaatagg ttacgacaag caaaacgaat gcttccagaa
    16621 gaaagtgaaa acgaaaggga aacacttgta aacgaactaa gcagactgga atatcaaaaa
    16681 gcagaacttc aaagagtaat gccaaaagaa cttgaagcta gtgaaatcaa tgtaaggctt
    16741 ggtgcaacat ggattccacc taaagatatt gaaagattcg tatttgaaac attaaagaca
    16801 ccaggatatg caagatggga tattaaagtt aaattttcgc atttaactag tgaatggaat
    16861 gttgaaggta agagcaaaga taggggaaat gacctcgcag aaatgactta cggtacaagc
    16921 agagtaagtg cctataagct tattgaagac gctctaaacc taaaagaaac aaaggtgttt
    16981 gaccaaatcc taaatccaga tggttcaaag acttcggtgc taaataagaa agaaaccatg
    17041 cttgcaggac agaaacaaga gcttataaaa gaagaattta agaactggat atttagtgat
    17101 caggatagaa gaagtagact agtaaaagcc tataatgaaa aatttaattc tattcgtaat
    17161 cgagaatatg acggatcaaa tttaagcttt gatggaatga gtacagatat ttcgcttcgt
    17221 tcacatcaaa agaatgcaat tgcaagaata ctttatggag gtaatagctt gcttgcccat
    17281 gtggtaggag ccggcaagac ttttgaaatg gtagcaagtt ctatggaagc aaagaaactt
    17341 ggaatgtgta ctaagtcatt atttgttgtg ccaaatcatc ttactgggca aattggtcgt
    17401 gaatttatgc agctataccc atcagctaat attatggtgg ccgataaaaa agactttgaa
    17461 ccaaagaatc gaaagagatt tatagggaaa atcgcaacag gtgaatatga tgcagttatt
    17521 attggtcata gtcagtttga aaaaattcca atgtccaagg aatatcaaca aaagcacatt
    17581 caagatcaga ttgatgagat tatcaattat gtagaagagt ataagcatga tcgaaatcaa
    17641 aactttacag taaaagagct tcaaaagact agaaagaaat tagaaacaag acttgaaaag
    17701 ttaaacgatg atttcaaaaa agatgatgtt attacttttg aagagctggg agtggataag
    17761 ctgattgttg atgaggcaca taattataag aacctatatc tttatacaaa aatgagaaat
    17821 gttgcaggta taggacaatc agaagccttt aagtcctccg atatgtttat gaaatgtcga
    17881 tacctggatg aaatgactgg tggcaaagga attgtatttg ctacaggaac tccagtatcg
    17941 aattcaatga cagagcttta tactatgcaa agatatttac agtacgatac tttgaaaaag
    18001 aatggattag agcattttga ttcatgggca tcaacctttg gagaaacaca atctgctttc
    18061 gagttatctc cagagggaac aggctacaga gtgaagactc gtttctctaa gttttataac
    18121 ctacctgagc ttatgagtat gtttaaggaa gtagcggata ttcaaactgc agatatgctt
    18181 aatcttcctg ttccagaagc tcattttaaa gtcattaaaa ctgaaccaag tgaagaacaa
    18241 aaagaaatac taaagagctt gtctgaaagg gctgataagg tgcgtaataa gtccgtagag
    18301 cctgaagaag ataatatgct taagattact aatgatggta agaaacttgc cttagatcag
    18361 cgtttaataa atcctttact tccagatgat gaaaactcaa aggttaatgt atgtgttaaa
    18421 aatgtatttt caatttggga taagacaaaa gaagaaaaat caacacaact tctattttcc
    18481 gatatgtcta caccaaaggg tgatggtagc tttaatatct atgatgatat tagagataaa
    18541 cttgtagact taggaatacc aaaggaagaa attgctttta tccatgaatc caatactgat
    18601 aagcaaaaag atgaactctt tgcaaaggta cgaaaaggag aaattcgtat tttaatgggt
    18661 tctactcaga agatgggtgc tggtacaaat gtgcaaaata aactaattgc aatgcatgat
    18721 ttagacgtac catggaggcc ggctgacctt gagcaaagaa gtggtagaat tgttcgccaa
    18781 ggaaatgaaa ataaagaggt aaatatttac cgttacatca ctgagaatac ctttgatgcg
    18841 tacctttggc agacgataga gaataagcag aaatttattt cacagattat gacctcaaag
    18901 acgcctgttc gtgtggcgga agatgtggat gaaagtagtc tgaactatgc tgagattaaa
    18961 gctcttgcta ctggtgatcc gaagataaaa gaaaagatgg atttggacaa tgaagtcacg
    19021 aaacttaaaa tgcttgaggc aaactataag tccaatcgat atagattaga ggataaggtt
    19081 gcaaagacat atcctgaaga aatagcaagg actgaaaagc taattgaagc agtgaaaaaa
    19141 gatattgaaa atgttgagcc acaaggaagt ggtgaaaata aattcacttc tatcacgatt
    19201 aatggtgaga taattcgaga taaaaagata gctagagaaa agctcttaga agcgattaaa
    19261 ggcgtaaaaa ttaatgaaag taaggttata ggacagtacc gaaatatgga tttagaggta
    19321 agctataact tttttaccaa tgaacataat ttcactttaa aaggtgctac aaaccattta
    19381 ggggaacttg gtacaagtgc agatggtaac attacaagac tggataatgc tattgataaa
    19441 atgccagaaa agttaaatcg tcttgaagaa aaactcgtta gtacaaagga acaactggaa
    19501 aatgcaaaag aagaattaac aaagcctttt gaaaaagcag atgagctaaa gacaaaagta
    19561 ttaagacttg cagaattgaa caagctactt gatatgggag aagtagaaga aaaagaaaat
    19621 cctaatccat tacttgaaga tgtaaagagg gctattgtag atttttgcaa aagggaatat
    19681 gaagatgata gttatactta tgaaaacttt aataatctat ttcctgacct tgctcatatc
    19741 ggaattgctt atacgactac accagatgaa aagcatgaaa ttcaatatga aattagctta
    19801 gaagacttta cagctactca atatatcaat ggagaaccaa ttacaaagat tgattatttc
    19861 aaagaccttg gaagtgaaga aaaggcatta gaatttctaa aacaagaaat ggaatatgga
    19921 gactttggag agtttgtatc tattgatgat aatgacctta aaaaagcatt aggtcttgaa
    19981 agggatgaag atggtaatat ctatgattca cttgcaaaag atttggacaa tgatggtgtg
    20041 gcagatagat acgataatga ctttagagat agtgactatt ttgaaactac ttatgatgta
    20101 gaggataata ttcacacaag agcagaaagc aaagaaaagg tatctgaaaa gccgtctatc
    20161 ttaggacaga ttaagtcata tcagtcagaa gaaaaacaag cagaaacaag agaaaataaa
    20221 tcaaaagaac acgaagaacg ttaaggggct tactttaagc tccttttcat ttttagtaga
    20281 aaaaatggag gtatattatg aactacaaag aaatgagaaa tgcactagaa caaatggcaa
    20341 acgaaaatca cgaagatttt gcaaaggcac taattagttt tgaaaaagga atcaatgata
    20401 aagatgcctt ggacaagtta taccaggagt atatggataa tgacagtatg agcttactca
    20461 atgatgagtt tgattactta attgacgaac ttagagaaaa tggacagatt aaagacagtg
    20521 cagcaattga aaaagaagat aataatttgg tgaatattgt tggaaatata gtaggtgagg
    20581 ttgaaacaat tgaaagagaa aataaaaatg gagaaacctt taaagtaatt aatttctcag
    20641 ttgtgtcaaa agatgatgag ggaaataaga tttacactaa ttgctcagct tatggggata
    20701 agggagatat tccaaagaac tttaagcagg gagactttgt aaagcttttt ggacaggtga
    20761 gatcatccat tgacgataac ggaaaggaac atactaatgt tagaatacta tcgtctaagc
    20821 ttctaaaagc aaaggaacag atgaagaatc aagaagaaaa gaaagaatca gtacttggag
    20881 caataaagaa atataaggct gaagaaaaag caaaacctgt agaaaagaaa aaatcttcaa
    20941 aggaaacgga gagatagaat atgtcggtgt ggtcttaggg ctgcaccgat ttttttattg
    21001 caaaattagt tcgagaaaaa agaagataat actattaaaa gttctattat tatgctataa
    21061 tagaagcgat gtgatattag gatggataag actggaggat aatcatgtca aaactaagct
    21121 ataaaaaatt atttaagaaa ttaatagata tagaaatgaa gaatactgag cttatggata
    21181 aagcaaaagt aagtaaaagc acattctaca aaataaaaaa tggcgaaaat gttactactg
    21241 atgtattgct aagaatatgt gatgtattgg aatgtgatat ttcagaaatt gtagagtgtg
    21301 taagtattga aaatggagga aaaacgggtg tctaagttta cagttataga tttattttca
    21361 ggagctggtg ggctatctaa aggattttta gacgcgggtt ttgatgtttt actaggtgta
    21421 gataatgata aagcggcttt aagcactttt gagttgaatc ataatggagc aaaagcacta
    21481 tctctggatc tgtctgataa aaaggtattt aaagatatag aaaacaccat tggaaataga
    21541 gagctagatg taattgttgc cggacctcca tgccaagggt tttctttaag tggtcctaga
    21601 aattttgatg atgagagaaa taaattatat ttagccgtaa ttaaaacagt aaaaactttt
    21661 aagccaaagg cgtttattat agaaaatgtg cctggaatgg caacgttgta taacggagaa
    21721 ataaagaacg aaattttgaa acgatttaaa aagatgggat ataacattag ttgtaaaatt
    21781 ttatgtgctg ctgactatgg agttcctcaa atgagaaaaa gacttatatt tatgggagtt
    21841 agaaaagatg tcggagagcc acattttcca gatccgatct atgatgagaa gaactatata
    21901 acatgtagag atgcaattag tgatttgcca tcaagagagt ctgaattggg tgaaaatacc
    21961 gatatatata atgattttcc aaaaactaag tatcaaatta aaatgagagg aaaatgtaaa
    22021 gttctaagta atcacgtagg cacgaatcat accacactcg taaaggaaac aattgcatta
    22081 gttcctgaag gaggtaatta taaagattta cctgaaggat ggggtgagag ccgaaaattt
    22141 aatgaggctt ggactagata tgatggaaat aaaccatcga agactattga tacgggacat
    22201 cgaaaccact tccattataa gtataataga gtacctacag tgcgagaaaa tgctaggtta
    22261 caatcttttc ctgatgactt cgtatttttg ggcacaaaaa cgcagcaata taggcaagta
    22321 ggaaatgcgg tgccaccatt attagggttt gcgttagcaa atgagttgat aaaaatacta
    22381 gagaaagagt ttaaataatg agcaaaaata caaataaaat aaagtcaata gatttatttg
    22441 caggttgtgg tggcttaatg gatggttttg agcaatctgg gttttataaa actttagctg
    22501 ctgtagaatg ggaaaaagca ccttgcgata atttaaaaat tagacttaaa gataaatgga
    22561 aatatcataa ttctgaagaa attgtaatgc aatttgacat acaaaggaca gatgaacttt
    22621 tcgaaggatg gaatgaggaa aagtatggag cttcaaaagg attagattat atagttaata
    22681 aaaatagtgg gattgacata ataataggag gcccgccatg tcaggcttat tccatagcag
    22741 gaagagttag agatgagaat ggcatgaaag atgactatag aaattattta tttgaaagtt
    22801 atttgaaagt agtaaaaaag tatagtccaa aattgttcgt gtttgagaat gttccagggt
    22861 tgttaagtgc gaaaccagga gacagaaata ttgtagacat tataaaagag caatttgagg
    22921 aagctggtta ttctatttta gaa
//
LOCUS       DAUDYX010000033        22703 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.033, whole genome shotgun sequence.
ACCESSION   DAUDYX010000033 DAUDYX010000000
VERSION     DAUDYX010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22703
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.033"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            100..855
                     /locus_tag="VHD02_001229"
     CDS             100..855
                     /locus_tag="VHD02_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ5497838.1"
                     /translation="MNILGNSIKEIRKSKKMTQKELAKLTGFKQNTISNHENGNRQLD
                     EVDIRKYAKALKIEPQQLFDYSSSPTNPQVELIPSTLQKITSTSSQLEHKRQLNVLDY
                     AETQLEQQNTVESNKDIVEEPQATYYTYNYYDHAASAGTGQYLNDVQVETIELPVDYD
                     ADFVIPVHGDSMEPKYHSGDYVFVKLSVELADGDIGVFEYYGDAYIKQLLINDEGAFL
                     HSLNSKYEDIPVDRDSDFRIIGEVVGSYSGNHS"
     gene            893..1573
                     /locus_tag="VHD02_001230"
     CDS             893..1573
                     /locus_tag="VHD02_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497839.1"
                     /translation="MKFWNLVVKTLKQKNNQAVAEKVVKITESTTNIKSPSYPDLNKY
                     RVNPSGKRYDDTYITGVGYKLREILLLVWWGRTKNPRKPTSKPPRYFFYDYHLNTKKT
                     TDMFIRDGLLKKNKEGCITLTLSGKVLYDEYKILWEIHSYKGYIGELPNMDRVFHGWN
                     YNSYKANNNLLEIRHLEDIVKYNTIMRDQYKKGSNEYNAFQQDIEQDKNQIALLFNEH
                     QLLENIDN"
     gene            1709..2848
                     /locus_tag="VHD02_001231"
     CDS             1709..2848
                     /locus_tag="VHD02_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEQ5497840.1"
                     /translation="MWSEKHKSGKVNFVERYKHPYTGKWCRASVLMEKDTPRIRKEAK
                     KYLDEKISKILTSLTTTDAYLLDVMNEWWEHHQKSLKSTSIRSLDFRIRELRNLIDPE
                     VMIAKITTKYLQSIIDKIPGSYDKRKRARQLLKQTFDYAIALEYVSINPVISTQLAKP
                     VKTIKDFEDVAQKFLEKDELKRLLDEMYRRKGSIKMAYLAEFMSLNGCRIGEALAIQP
                     DNIKNDIIEIHGTLDYTSNGYRNAIKTTPKTNSSWRETLITKREKEIIQDILKINALE
                     KNTNPNYKDMGYIFISRNGVPIQDNALNTSIRAANKRLEKPIQKELTSHIFRHTLVSR
                     LAENKVPLKTIMDRVGHADSKTTQQIYTHVTKSMKNEVVDILNRL"
     gene            complement(2942..3982)
                     /gene="rfbB"
                     /locus_tag="VHD02_001232"
     CDS             complement(2942..3982)
                     /gene="rfbB"
                     /locus_tag="VHD02_001232"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEQ5497841.1"
                     /translation="MYKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGNRA
                     NIEAILGDRVELVVGDIADAELVDKLAAKTDAIVHYAAESHNDNSLEDPSPFIHTNFI
                     GTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGQGEGPGEKFTAETKYNPSSPYS
                     STKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLYGE
                     GKNVRDWIHTNDHSIGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKDAY
                     DHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKDAV
                     EAKYAKTQEVIK"
     gene            complement(4226..4819)
                     /locus_tag="VHD02_001233"
     CDS             complement(4226..4819)
                     /locus_tag="VHD02_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEQ5497842.1"
                     /translation="MTETFFDKPLACREIKEIPGLLEFDIPVRGDNRGWFKENFQKEK
                     MLPIGFPERFFEEGKLQNNVSFSRQHVLRGLHAEPWDKYISVADDGKVLGAWVDLREG
                     ETFGNVYQTVIDASKGMFVPRGVANGFQVLSETVSYSYLVNDYWALDLKPKYAFVNYA
                     DPSLGITWENLAAAEVSEADKNHPLLSDVKPLKPKDL"
     gene            complement(4819..5688)
                     /gene="rfbA"
                     /locus_tag="VHD02_001234"
     CDS             complement(4819..5688)
                     /gene="rfbA"
                     /locus_tag="VHD02_001234"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEQ5497843.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSTLMLA
                     GIKDILIISTSQDLPRFEELLGDGSEFGISLSYKEQPSPDGLAQAFIIGEEFIGDDRV
                     ALILGDNIYHGNGLTKMLQKAAAKEKGATVFGYQVKDPERFGVVEFDENMNAISIEEK
                     PEVPKSHFAVTGLYFYDNDVVEIAKNIKPSARGELEITDVNKAYLERGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRLQNAQVANLEEIAYRMGYISKEDVHELAQSLKKN
                     EYGQYLLRLIGEA"
     gene            complement(5746..6852)
                     /locus_tag="VHD02_001235"
     CDS             complement(5746..6852)
                     /locus_tag="VHD02_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEQ5497844.1"
                     /translation="MKIAIIGAGIVGSTAAYYLQQSGQKEVTIFDHGQGQATKAAAGI
                     ISPWFSKRRNKVWYRMARLGADFYQQLINDLKEDGFATDFYQQNGIYVLKKQEEKLRD
                     LYELALARKVESPIIGELAIKNRKELGNDFKGLIGFDNCLYASGAARVEGATLCETLL
                     KASGYPVIRQKVTLKQQGSGYEIAGHYFDQVILAAGAWLPDLLRPLGYQVDVRPQKGQ
                     LLDYDVHHIISDNYPVVMPEGEIDLIPFNQGKISVGASHENDKGYDLEPDWQVLEKLE
                     MQALTYLPLLKEATQKTCRVGIRAYTSDYSPFYGQVPGLKNFYTASGLGSSGLTVGPL
                     IGYELAQLLLGHEGLLTPSDYSPEPYLLRSNGKA"
     gene            complement(6887..7675)
                     /locus_tag="VHD02_001236"
     CDS             complement(6887..7675)
                     /locus_tag="VHD02_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019781659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEQ5497845.1"
                     /translation="MKAKTLIDAYEAFCPLDLSMEGDVKGLQMGSLDKDIRKVMITLD
                     IRESTVAEAIKNEVDLIITKHAPIFKPLKDLVSSPQRDILLDLVKHDISVYVSHTNID
                     IVPGGLNDWFCDLLEIKEATYLSETKEGFGIGRIGTVKEQALEELASKVKRVFDLDTV
                     RLIRYDKENPLISKIAICGGSGGEFYQDAVQKGADVYITGDIYYHTAQEMLTEGLFAV
                     DPGHHIEVLFTEKLKEKLQGWKEENGWDVSIISSKASTNPFSHL"
     gene            complement(7665..8351)
                     /locus_tag="VHD02_001237"
     CDS             complement(7665..8351)
                     /locus_tag="VHD02_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEQ5497846.1"
                     /translation="MDSQLSNRLAQVAAYVPKGVKLLDVGSDHAYLPIFLVETNQISA
                     AIAGEVVRGPYESALKNVTQSGLAEHIQVRLANGLAAFEEADDVTAITICGMGGRLIA
                     DILEAGKEKLQGIERLVLQPNNREDDLRAWLSVNAFKIVAETIMAENDKYYEIIVAEH
                     GEKALSATELRFGPYLSQEKSVVFKEKWQREMDKLAYALSCIPEEKTQERQLLLTKIQ
                     QIKEVIVHES"
     gene            complement(8423..9079)
                     /gene="nth"
                     /locus_tag="VHD02_001238"
     CDS             complement(8423..9079)
                     /gene="nth"
                     /locus_tag="VHD02_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEQ5497847.1"
                     /translation="MRIGKARLAKVLTIIGQMFPEAKGELDWETPFQLLIAVILSAQT
                     TDKAVNKVTPGLWQSYPEIEDLAFAELSDVENALRTIGLYKNKAKNIIKTAQAIRDDF
                     KGQVPKTHKELESLPGVGRKTANVVLAEVYGVPAIAVDTHVARVSKRLNISSPDADVK
                     QIEADLMAKIPKKDWIITHHRLIFFGRYHCLAKKPKCEICPVQSYCKYYQNTYGKSKA
                     "
     gene            complement(9076..9759)
                     /locus_tag="VHD02_001239"
     CDS             complement(9076..9759)
                     /locus_tag="VHD02_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEQ5497848.1"
                     /translation="MSFLEHYKSGNLVIPSALLFHYKDLFKSSDDFLVWQFFYLQNTT
                     KRDDLAPSQIAHALGKSVADINKIISSLTSQGLLDMRTIELTGEIEIIFDASPVLAKL
                     DQLFVSQTATEIDKQETPNHFKRLVDEFERELGRFLSPFELEDLEKTLRDDKTDPDLI
                     REALKEAVFNGKTNWKYIQAILRNWRKEGIVNLRQVEERRRVREGEDLSQVTISEDFL
                     SAMNLWSDS"
     gene            complement(9840..10358)
                     /locus_tag="VHD02_001240"
     CDS             complement(9840..10358)
                     /locus_tag="VHD02_001240"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEQ5497849.1"
                     /translation="MDLTNYIASIKDYPKAGITFRDISPLMADGKAYSYAIREIAQYA
                     CDKDIDMVVGPEARGFIIGCPVAVELGIGFAPVRKPGKLPRDVVSADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGIVAGCAFLIELEGLNGRHAIR
                     NYDYKVLMQFPG"
     gene            complement(10509..12719)
                     /gene="recJ"
                     /locus_tag="VHD02_001241"
     CDS             complement(10509..12719)
                     /gene="recJ"
                     /locus_tag="VHD02_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEQ5497850.1"
                     /translation="MIKSKYSWKIKDKKPDDGFFKLAKTKGLTQTAAQLIYDRGIRTE
                     EALDEFLTADLSQLHDPYLLHDMAKAVPRIRQAIEEGERILIYGDYDADGMTSASIVK
                     ETLDMMGAEPLVYLPNRFTDGYGPNQSVYKYFIEQEAVSLIITVDNGVAGHEAIRYAQ
                     EQEVDVIVTDHHSLPEELPEAFAIIHPEHPDADYPFKHLAGCGVAFKLATALLESLPT
                     DCLDLVAIGTIADMVSLTGENRVLVKNGLAMLKHTERVGLQELMSLSPIDLEHFNEDA
                     IGFQIAPQLNALGRLDDPNPAIELLTGFDDQEAQAIALMIKKKNEERKALVQDIFDQA
                     MAMVDPQKPVQVLAQAGWHPGVLGIVAGRIMETIGQTVVVLTIDNGFAKGSARSLEAI
                     NIFEALNGKRELFTAFGGHAGAAGMTLPVDNLEALSDFLCQFVIERGLDQTAKNTLTI
                     DERLSLDDLSLDILKSLDKLAPYGMDHQKPVFYVKDIRVSQARTIGQDQSHLKFKVSQ
                     GKASFDVLAFGQGSQLQEFRQATGLELAVTLSVNHWNGNTSLQFMLVDARVDGVQLLD
                     LRTKTAKVPEGIPTIEEDPNARVILINDIPEDFKTWRNQFVHKDFDAIYFKNQMKHPY
                     YLTGFGSREQFAKLYKTIYQFPEFDLRHKLTELSHYLNIEKLLLIKLIQIFEELSFVT
                     IDDGLMTVNPQAQKREISESHIYQDLKELVKFQEIMALASPKEMYDYLVKSNDQ"
     gene            complement(12716..13480)
                     /locus_tag="VHD02_001242"
     CDS             complement(12716..13480)
                     /locus_tag="VHD02_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEQ5497851.1"
                     /translation="MAQRIIVITGASGGLAQAIVKQLPKEDSLILLGRNKERLEHCYQ
                     HIDNKECLELDITNPVAIEKMVAQIYQRYGRIDVLINNAGYGAFKGFEEFSAQEIADM
                     FQVNTLASIHFACLIGQKMAEQGQGHLINIVSMAGLIASAKSSIYSATKFALIGFSNA
                     LRLELADKGVYVTTVNPGPIATKFFDQADPSGHYLESVGTFTLQPNQVAKRLVSIIGK
                     NKRELNLPFSLAVTHQFYTLFPKLSDYLARKVFNYK"
     gene            complement(13480..14409)
                     /locus_tag="VHD02_001243"
     CDS             complement(13480..14409)
                     /locus_tag="VHD02_001243"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEQ5497852.1"
                     /translation="MELQFLGTGAGQPAKQRNVSSLALKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKIRKIFITHLHGDHIFGLPGFLSSRSFQASEEQTDLDIYGPIGIKTYV
                     LTSLKVSGARVPYQIHFHEFDDKSLGKIMETDKFEVYAERLAHTIFCMGYRVVQKDLE
                     GTLDAEALKAAGVPFGPLFGKIKNGQDVELEDGRLICAKDYISAPKKGKIITIIGDTR
                     KTSASVKLAKDADVLVHESTYGKGDERIARNHGHSTNMQAAQIAHEAGAKRLLLNHVS
                     ARFLGRDCRQMEKDAATIFENVKMVQDLEEVII"
     gene            complement(14424..15059)
                     /locus_tag="VHD02_001244"
     CDS             complement(14424..15059)
                     /locus_tag="VHD02_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEQ5497853.1"
                     /translation="MNNYIDLAKTYGGFTSLDTNYLNHLLASLTDQQKLAFITPPPSV
                     INAYFAEIYQKQSPQAATDYYFNLSKALGLFTDQPSFEEEKPFVRLNLSGKAYGFAYQ
                     NDQEVALVFSEKAEPKKPELFFELTQIFPQYMVYEDKGQLKMQAKQFEQGECEDITPD
                     DTLLSKIYRLANGITMLKGFNVEELWALSQTFSGQKYYDFAQREFMIYITQ"
     gene            complement(15052..16290)
                     /gene="hflX"
                     /locus_tag="VHD02_001245"
     CDS             complement(15052..16290)
                     /gene="hflX"
                     /locus_tag="VHD02_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEQ5497854.1"
                     /translation="MIETKRQQERVILLGVELQTTEHFDMSMTELANLAKTAGVKVMA
                     SFSQKRERYDSKTFIGSGKLDEIKAIVEADEIDAVIVNNRLTARQNANLEAVLEVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQIADIERQLTQVEKNRQTIRERRVGSDTFKIGLIGYTNAGKSTIMNLL
                     TDDSHYEANELFATLDATTKQLYLENQFQATLTDTVGFIQDLPTELVAAFKSTLEESK
                     YVDLLLHVIDASDPNHSEQEKVVLNLLKELDMLNIPRLAIYNKVDIAEQFTATAFPNI
                     RISARSKDSKILLRRLIIDQIRDQFVPFRIKVHQDKAYKLYDLNRVALLDHYTFDQEI
                     EDISGYISPKQQWRLDDFYE"
     gene            complement(16363..17262)
                     /gene="miaA"
                     /locus_tag="VHD02_001246"
     CDS             complement(16363..17262)
                     /gene="miaA"
                     /locus_tag="VHD02_001246"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEQ5497855.1"
                     /translation="MTKIKIVVIVGPTAVGKTALGISLAKAFNGEIISGDSQQVYRQL
                     DIGTAKATQEEQEAAVHHLIDIREVTESYSAYDFVQDAQKAISDIVSRGKLPIIVGGT
                     GLYLQSLLEGYHLGGQVDQEAVKAYRNELEQLDDHDLYERLQVNNITIEQVNRRRAIR
                     ALELAQFADELENAETAYEPLIIGLNDDRQVIYDRINQRVNRMLENGLLEEAKWLYEH
                     YPTVQASRGIGYKELFPYFVGEMTLAEASDQLKQNTRRFAKRQLTWFRNRMAVSFTAI
                     TAPDYPQVVHDRVRDFLGQKEKS"
     gene            17396..17572
                     /locus_tag="VHD02_001247"
     CDS             17396..17572
                     /locus_tag="VHD02_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEQ5497856.1"
                     /translation="MVKKYQFVKGLATGVLATAATVAGAVFAVKKTIIDPEEEKAAFI
                     EENRKKAARRRVAR"
     gene            complement(17690..18583)
                     /locus_tag="VHD02_001248"
     CDS             complement(17690..18583)
                     /locus_tag="VHD02_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEQ5497857.1"
                     /translation="MKHLKNPPLVMSGLALGTLSFGNLLATYVSIFSYLGILAALFIY
                     GILLVGMVRNLNDTKMQLRQPLIASVFPTFFMTGVLLSSLFLKVTGGCWLGFLTWWLF
                     FLGNLVLIAYYQYRFVFSFSWDNVFPSWSVLFVGIAMAALTAPASRQFLLGQVIFWVC
                     LPLTAVILPFMAKKTYGIGLGQAVMPNISTFCAPLSLLSASYLATFPRPQVGMVIFLL
                     ISSQLLYAFVVVQLPRLLNRPFNPGFSAFTFPFVISATSLKMTLSFLGWQVLLLGEVL
                     LATALVTYVYGAYLRFLFQNK"
     gene            complement(18642..18944)
                     /locus_tag="VHD02_001249"
     CDS             complement(18642..18944)
                     /locus_tag="VHD02_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEQ5497858.1"
                     /translation="MDFLTGDFPLVFRPMYNPHRYTISQDNQALEKVKQASYKRMDIA
                     MTHLDGLIGDSGHVYRDQQTIADAYAYAMALWSQKTPKSYENYPHLAAFMAKMLEM"
     gene            complement(19339..20325)
                     /locus_tag="VHD02_001250"
     CDS             complement(19339..20325)
                     /locus_tag="VHD02_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEQ5497859.1"
                     /translation="MSEKIRVLLYYKYVSIENAQEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWVHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWLVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENETKYVRGCSPECRAHERNRYVQENGLSRQEWAERLEAIGESLPEFVGA"
     gene            20525..20827
                     /locus_tag="VHD02_001251"
     CDS             20525..20827
                     /locus_tag="VHD02_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497860.1"
                     /translation="MTYELCLEYGTYPLSRVYAYWGEDQNPPTFIQEDRLLCHKLETM
                     NHLFHDLFVTIESQFHYVGFNMPEKRAQIRILYQEVATILKSKYKDYPIKIETFLL"
     gene            complement(21190..21261)
                     /locus_tag="VHD02_001252"
     tRNA            complement(21190..21261)
                     /locus_tag="VHD02_001252"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21226..21228),aa:Arg,seq:ccg)
     gene            complement(21374..22579)
                     /gene="rpsA"
                     /locus_tag="VHD02_001253"
     CDS             complement(21374..22579)
                     /gene="rpsA"
                     /locus_tag="VHD02_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEQ5497861.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIEGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVIEEAAKEARAEVFSKISEGAVVTGTVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVSVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AQGDVVEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSVGQEVTVKVL
                     EVNAADERVSLSIKALEERPAQAEGDNKEEKRQSRPRRPKRESRRDYELPETQTGFSM
                     ADLFGDIEL"
BASE COUNT         7032 a         4855 c         4015 g         6801 t
ORIGIN      
        1 gagatatttt gtcaactatt ttttatctct ttttgagatt tttttattta cttttttgtt
       61 tcttgatgtt atatttatag tagagaaagg aaaaatatta tgaacattct cggaaattct
      121 atcaaagaaa ttcgaaaatc caaaaaaatg acccaaaaag agttagcaaa actaactgga
      181 tttaaacaaa atacaatttc taatcacgaa aatggtaata gacaacttga cgaagtagat
      241 attcgaaaat atgctaaagc actgaaaata gagccacaac agctttttga ctattcctct
      301 tctcccacaa acccccaagt tgaactcatc ccatctactc tacaaaaaat cacttctact
      361 tcttctcaat tagaacacaa gcgacaacta aacgttcttg attatgccga aacacaatta
      421 gaacaacaaa acacagtaga aagcaataag gatatagtag aagaaccaca agccacctac
      481 tacacctaca attactacga ccacgcagct tcagctggta caggtcagta tctaaatgat
      541 gtacaagtag aaacaattga attaccagtc gattatgacg ctgattttgt cataccggtt
      601 catggcgatt ctatggaacc aaagtatcac tctggggact atgtgttcgt taagctatcc
      661 gtagagctcg cagatggcga tataggcgtc tttgaatact atggtgacgc ttatatcaaa
      721 cagctactta taaatgacga gggggcattt ctgcacagtt taaatagcaa gtatgaagat
      781 atacccgtag atagagatag cgactttaga attattggtg aagtcgttgg aagttattcg
      841 ggaaatcata gctgatgagt gatagttaaa gaatttaggt taggagattg atatgaagtt
      901 ttggaatttg gtagttaaaa ctctaaaaca gaaaaataat caggctgtag ctgaaaaggt
      961 tgtaaaaatt accgaatcga ctactaatat aaagtcacca agctatcccg acttaaataa
     1021 gtatagagta aatccaagcg ggaaaagata tgatgataca tacataacag gtgtaggata
     1081 caaactacgt gagattttat tgttggtgtg gtgggggcgt actaaaaacc ccagaaaacc
     1141 gacaagtaaa ccaccacgct acttcttcta cgattatcat ttaaatacta aaaaaactac
     1201 agatatgttt attcgagatg gattgcttaa aaagaataaa gaagggtgta ttactttaac
     1261 actatcgggg aaagttcttt acgatgagta caaaatcctc tgggaaatac attcgtataa
     1321 aggatatatc ggtgaattac caaacatgga tagagtgttc cacggatgga attacaactc
     1381 ttataaggcc aataacaatt tattagagat tagacattta gaagacattg taaaatacaa
     1441 tacaattatg agagatcaat ataaaaaagg tagcaatgaa tataatgcgt ttcaacaaga
     1501 tattgagcaa gataaaaacc agatagcact attgttcaac gaacatcaat tgcttgaaaa
     1561 tatcgataat tagttaatct aaaacaaaaa accccacgct ctcaaagttt ggcgactctg
     1621 agcgtgaggc aagacagtat agtaaaaacc tgcttcgcag taggtctctt tactatacct
     1681 attttaacag aaaatgaggt aaaaaacaat gtggtcagaa aaacacaaaa gcggaaaagt
     1741 aaattttgta gaacggtaca aacacccgta tactggcaag tggtgcagag cttctgtcct
     1801 tatggaaaaa gacacgccac gaatacgaaa agaagccaaa aaatatcttg acgaaaaaat
     1861 atctaaaatc ttaacaagtc ttaccacaac cgatgcctac ctactagatg tcatgaacga
     1921 atggtgggaa catcatcaga aatcactcaa atcaacttct atccgctctc ttgattttag
     1981 aataagagaa ttgcgaaact taattgatcc agaagtgatg attgccaaga taaccacgaa
     2041 atacctgcaa agtattatcg acaaaatacc aggtagttat gacaaacgta aacgagcacg
     2101 gcagctgctt aaacaaacct tcgactacgc tatcgcttta gaatatgtct caatcaatcc
     2161 ggttattagt acccaactcg caaaacctgt taagaccatc aaagactttg aagacgttgc
     2221 ccagaagttt ttagaaaaag atgaactcaa aaggctatta gatgaaatgt atagacgtaa
     2281 aggatcgatt aaaatggctt atctggcaga atttatgagc ttaaacggct gccgcattgg
     2341 agaagctcta gctatacagc cagacaatat caaaaatgat atcattgaaa tccacggaac
     2401 tcttgactac acctcaaatg gctatagaaa cgctatcaaa accaccccta aaacaaattc
     2461 cagttggcgt gaaacattga taaccaaacg tgaaaaagaa attattcagg atatactgaa
     2521 aattaatgct cttgaaaaaa atactaatcc taattacaag gacatgggct acatcttcat
     2581 ttccagaaat ggcgttccta ttcaagacaa cgccctcaac acctctatca gagcggcaaa
     2641 taagagacta gaaaaaccga ttcaaaaaga actcaccagc cacatctttc gtcataccct
     2701 ggtgagtcgc cttgcagaaa acaaagtgcc cttaaaaact atcatggata gagttggaca
     2761 tgctgactcg aaaactactc aacaaatcta tacccacgtc acgaaaagca tgaaaaatga
     2821 agtcgtcgac atcttaaacc ggctataata taccccaaat atgccccaaa aatgccccaa
     2881 aacacaagaa aaaaccttga tatcttcaac aaacgttgat atgacaaggt tctttaatgt
     2941 tttatttaat cacttcttga gttttagcat acttggcttc tacagcatct ttttctgctt
     3001 tccaccatgt ctcattttct gtataccact taatagtttc ttccaaacct tctgaaaagt
     3061 ttgtaaattg tggttcccag ccgagttctt cacgcaattt tgtagaatca atagcgtaac
     3121 gaagatcgtg accagcacgg tcagttacgt gatcataagc gtcttttggt tgacccattt
     3181 tctcaaggat aagctcaaga acttccttgt tgtttttctc gccgtcggca ccaataaggt
     3241 atgtttcacc gatacgaccc ttagtcaaaa tagcccatac tcctatagaa tgatcattag
     3301 tatgaatcca gtcacggaca tttttccctt caccgtatag ttttggcttg atgcctgata
     3361 aaatattggt gatttggcga ggaataaatt tctcaatgtg ctggtaaggt ccgtagttat
     3421 tggaacaatt tgaaatggtc gctttcacac cgaaggaacg tacccatgcc ttaacgataa
     3481 ggtcagaagc tgccttagtt gatgagtaag gtgatgatgg attgtatttt gtttcagcag
     3541 tgaatttttc acctggtcct tcaccctgtc ctggaaggtc ttcacgaagt ggaagatctc
     3601 cataaacttc atcagttgac acgtggtgga aacggatatc gtatttacga gctgcttcaa
     3661 gcaaagtgta agttccgata aagtttgtat ggataaatgg acttggatct tccaatgagt
     3721 tatcgttgtg actctcagcc gcatagtgaa caatagcatc cgttttggca gccaatttgt
     3781 ctaccaattc agcgtcagcg atatcaccaa caactaactc aacacgatca ccaagaatag
     3841 cttcaatgtt agcacggtta cctgcatatg tcaatttatc aaggacagtt acatgaacat
     3901 ctgggtggtt attgtagaca tagtgcacaa agttagatcc gatgaaacca gctccaccag
     3961 ttacgataat atttttatac ataagatata aagggacctt tcgtttccga aaaaatctat
     4021 taaaagtaat aactctaaca aagacgtttt aggcctaaga aaacatcgct ttctaagatt
     4081 tgagtcccat gttcagctca aagagagaaa ctgaactttc ctttcatcat ttttagttgt
     4141 tacttgttac ctgaagtaga gaagaataac tcccctcata tccacctcaa atgattaatc
     4201 ccaatcatct gagtatgttg tttccctata ggtcttttgg tttcagtggt ttgacatcac
     4261 taagaagagg atggttttta tctgcttcag aaacttctgc agctgctaga ttttcccaag
     4321 taatcccaag acttgggtca gcatagttta caaaagcata cttaggtttc aagtcaagag
     4381 cccagtagtc attgacaaga taactgtaag agacagtctc tgaaagaact tgaaagccat
     4441 tagcaacccc tctaggaaca aacattcctt ttgaagcatc aatcactgtc tggtagacgt
     4501 ttccaaaagt ctctccctca cgaagatcaa cccaagctcc taaaaccttg ccatcatcag
     4561 caactgagat gtatttatcc caaggttcag catgaagtcc acgcagcaca tgctgacgtg
     4621 aaaaagaaac gttgttttgt agttttcctt cctcaaaaaa acgttctgga aagccaattg
     4681 gcaacatttt ttctttttgg aaattttctt taaaccagcc acgattatcc ccacgaacag
     4741 gaatatcaaa ctctagtaag cctgggattt ctttgatttc tcggcaagcc aatggtttgt
     4801 caaaaaaagt ttctgtcatt aagcttctcc aatcaaacga agcaagtatt gcccgtattc
     4861 gtttttcttt aaagactgcg ccaattcatg gacatcttct ttactgatgt aacccatgcg
     4921 ataggcaatt tcttccagat ttgccacttg agcgttttgc aaacgttgaa ctgtttcaat
     4981 atattgagcc gcttcaagta gactttcatg cgttccagtg tctagccatg caaaaccacg
     5041 ccccatcaat tcaacggaga ggtcaccacg ttccaagtaa gccttgttga cgtctgtaat
     5101 ttccaactca ccacgcgcgc ttggtttaat gtttttagca atttctacca cgtcattgtc
     5161 atagaaataa agtccagtca ctgcaaagtg ggacttaggc acttctggtt tttcttcgat
     5221 agagatagca ttcatgttct catcaaattc caccactcca aagcgctcag ggtctttcac
     5281 ttgataacca aaaacggtgg ctcctttttc tttggcagct gccttttgaa gcattttagt
     5341 gaggccatta ccgtggtaaa tattatctcc taaaataaga gcaacacggt catcaccgat
     5401 aaactcctct ccaataatga aagcttgtgc taaaccatct gggcttggtt gttccttata
     5461 ggataagcta atgccaaatt ctgacccatc tcctagcaat tcttcgaaac gaggaagatc
     5521 ttgggaagtt gaaatgatta aaatatcttt aatccctgcc aacatcaagg ttgacaatgg
     5581 gtaataaatc attggtttgt cataaatggg catcaactgt ttagatgcgg cacgggtcag
     5641 tggatacaag cgtgtccctg aacctcctgc aagaataata cctttcatag tagtgctcct
     5701 attcttctat ataccctatc attctatcat tttttgaaag cagtgtcacg ctttaccatt
     5761 agaccgcaaa aggtaaggct caggggaata gtcgctaggc gttaaaagtc cttcgtgtcc
     5821 tagcaataat tgagctaatt catagccaat caaaggtcca actgttaatc ccgatgaccc
     5881 taaaccacta gctgtataaa agttcttcaa tcctggaacc tgaccataaa aaggagaata
     5941 atcactggta taagctctta ttcctacacg acacgttttt tgagtagctt cttttaataa
     6001 cggcaaataa gtaagagctt gcatttcaag tttttctaaa acctgccaat caggctccaa
     6061 atcatagcct ttatcatttt cgtgactcgc accaacagaa atcttacctt gattgaaagg
     6121 aataagatct atttcacctt ctggcataac aactggataa ttgtcgctga tgatgtgatg
     6181 aacgtcataa tccaagagtt gtcccttttg tggtcgcaca tcaacctgat agcctaaagg
     6241 cctcaataag tcaggtagcc atgctcctgc tgccagaatg acctgatcaa aataatgacc
     6301 agcaatctca taaccacttc cttgctgttt cagagtgact ttttggcgaa taactggata
     6361 accactagcc ttgagtaacg tctcacataa agtagcccct tcaactctag cagctcctga
     6421 agcgtacaga cagttatcaa agcctatcaa tcctttaaaa tcattcccca gttctttacg
     6481 atttttaatg gctaactcac caataatcgg tgattctacc tttcttgcta acgctaattc
     6541 ataaagatct cttagttttt cttcctgctt ttttaaaaca tagatgccat tttgctgata
     6601 aaaatctgtt gcaaaaccat cttctttgag gtcattgatt aattgctgat aaaagtccgc
     6661 tcctaaacga gccatgcggt accaaacttt attgcgacgc tttgaaaacc aaggactaat
     6721 aataccagca gcagccttag tggcctgtcc ttgaccatgg tcaaaaatgg taacttcttt
     6781 ttgaccactt tgttgtaaat aataagcggc ggttgatcct acaatccccg ccccaataat
     6841 ggctattttc attcctggta tccttttatt ttaagacctt aacagcctat aaatggctaa
     6901 atggattggt cgaggctttg cttgaaataa tgctgacatc ccaaccgttc tcttctttcc
     6961 acccttggag tttctccttt aatttctccg taaaaagcac ctcaatatga tgccccgggt
     7021 caaccgcaaa tagtccttct gtcagcatct cctgtgctgt atggtaatag atatcccctg
     7081 taatataaac gtctgcgcct ttttgcacag catcctgata aaattcacca ccacttccgc
     7141 cacagatagc aatctttgaa atcaaagggt tttccttatc ataacggata agtcgcacgg
     7201 tatctaaatc aaacaccctt ttgaccttac ttgctaactc ttctaacgct tgttctttaa
     7261 ctgttccaat acgaccaatg ccaaagcctt ctttggtttc agacaagtaa gttgcttcct
     7321 tgatttctag caggtcacaa aaccaatcat ttagcccacc tggtacaatg tcaatattgg
     7381 tatggctcac atagacagaa atgtcatgct tgaccaaatc tagaaggata tcacgctgag
     7441 gggatgaaac cagatcttta agcggtttaa agatgggagc atgcttagtt ataatgagat
     7501 caacttcatt tttgatggct tctgctactg ttgactctcg gatatctaaa gtaatcataa
     7561 ccttacgaat gtctttatcc aatgaaccca tttgaagacc tttaacgtct ccttccatag
     7621 ataaatcaag tgggcaaaag gcttcatagg catcaattag ggttttagct ttcatgaaca
     7681 atcacctctt ttatttgttg tatttttgtc aagagaagct gacgctcttg agttttttct
     7741 tctgggatgc aggacaaggc ataagctaac ttatccattt ctcgttgcca cttctcttta
     7801 aaaacgactg atttttcctg agaaaggtat ggaccaaaac gcaattctgt cgcggataag
     7861 gccttttcac catgctcagc aacgatgatt tcataatatt tgtcattttc agccatgatg
     7921 gtttctgcaa caattttaaa agcgttaacc gataaccaag cccgtaaatc atcctcacga
     7981 ttgttgggtt gtaaaaccag acgttcaatg ccctgcaatt tttccttacc tgcctctaaa
     8041 atatcagcaa tcaaacggcc acccatgcca caaatggtaa tggctgtcac atcatcagct
     8101 tcttcaaagg ctgcaaggcc attggctaag cgcacctgaa tatgctcggc aaggccagac
     8161 tgtgtgacat ttttaagagc agattcgtag ggacccctaa ccacttcgcc agcaatggct
     8221 gctgaaatct gattagtttc taccaaaaat atgggaagat aggcatgatc actgccaaca
     8281 tctaataatt tgacaccttt tggaacatag gccgcaactt gggccaaacg gtttgataat
     8341 tgactatcca tcttgatttc tttctaaaac gtaatgattc tattatagca aaattacctc
     8401 cataaaaaaa gaaaaccaaa ggctaggctt tcgattttcc ataagtattt tgatagtact
     8461 tgcagtaaga ctgaacggga caaatctcac attttggttt tttggctaaa caatggtagc
     8521 gtccaaagaa aatcaatcga tggtgagtaa taatccaatc tttctttgga attttagcca
     8581 tcaaatctgc ttcaatctgc ttgacatcgg catctggtga tgaaatattg agcctctttg
     8641 agactctagc cacatgagta tcaaccgcaa tagctggaac accataaacc tctgccaata
     8701 ccacattggc tgtttttcgg ccaacacccg gtaaactttc aagttctttg tgggtttttg
     8761 gcacttgacc cttaaaatca tcacgaatag cctgagcagt tttaatgata tttttggcct
     8821 tatttttata gagtccaatc gttctcagag cattttcaac atcagaaagt tcagcaaaag
     8881 ctaagtcttc tatttctgga taagactgcc atagtccagg agttacctta ttgacggcct
     8941 tatctgtcgt ttgagctgat aaaatcaccg caatcaacaa ttgaaaaggt gtttcccagt
     9001 ctaactctcc cttagcctca ggaaacattt gaccaatgat ggtcaggact tttgctaatc
     9061 ttgcttttcc aattctcatg aatcactcca taaattcatc gcagatagaa aatcctctga
     9121 tatcgtcact tgagaaagat cttcgccttc tctaacacgt ctacgttctt ctacttgtcg
     9181 aagattaaca ataccttctt ttcgccaatt acgtaagata gcctggatat atttccaatt
     9241 tgttttgccg ttgaagacag cttcttttaa agcttctcgt atcaaatcag gatctgtttt
     9301 atcatcacga agcgtttttt caagatcttc taactcaaaa ggactcaaga atcgtcctaa
     9361 ttcacgttca aattcatcaa ctaatcgttt gaaatgattt ggtgtttctt gtttgtcaat
     9421 ttcagttgcc gtttgactga caaacaattg atctaacttg gctaacacag gactcgcatc
     9481 aaaaataatt tcaatttctc cagtaagttc aattgtccgc atatctaaaa gcccttgact
     9541 agtcaaagat gaaatgattt tattaatatc agcaactgat tttccaagcg catgagcaat
     9601 ttgactgggc gctaagtcgt ctcgctttgt cgtattttga agatagaaaa actgccagac
     9661 caaaaaatca tctgagctct taaaaagatc tttgtaatga aaaagcaagg cacttgggat
     9721 aaccaaatta cctgacttat aatgttctaa aaaactcata atccctcaat caaaaggcta
     9781 aaatcgaatc tatagccttt actactttaa atcccctaat cagatgctga ttaatgtgct
     9841 tacccaggaa actgcattaa gactttatag tcatagttcc taatagcatg acgaccattt
     9901 aaaccttcta attcaataag gaaggcacaa ccagctacaa taccaccaag tttttcaatc
     9961 atttcaatag ttgcttttac cgtaccgcca gtcgctagta aatcatcgac aatcaaaaca
    10021 cgttgtccag gcttaatagc atctgcatgc atagttaaag tatcaagacc atattctttt
    10081 tcgtaatcgg cagagacaac atctcttggc agttttcctg gtttacgaac gggagcaaaa
    10141 ccaattccta actcaactgc aacaggacaa ccaatgataa aaccacgcgc ttcaggtcca
    10201 acaaccatat caatatcttt atcacaagcg tactgtgcta tttcacgaat agcatagcta
    10261 tatgctttac cgtcagccat taaaggtgag atatctcgaa acgtaatacc agctttaggg
    10321 taatctttga tcgatgcaat gtaatttgtt aaatccatag ttaattcttt ctagtggcaa
    10381 attgccaaat ataaaatact cttcattata tcacgaaaca tatttttcgt aaaataagac
    10441 ttctgacatt attgtttatc tgatacttca ttcgtaacta cctcaataac tactgtcaac
    10501 tgctttactt attgatcgtt tgatttcacc aaataatcat acatctcttt cgggctagcc
    10561 aaagccatga tttcttgaaa tttcaccaat tcttttaagt cttggtaaat gtggctctct
    10621 gaaatctctc gtttttgcgc ttgggggtta accgtcatca gcccatcgtc aatcgtcaca
    10681 aaagacaact cttcaaaaat ttgaatcaac ttaatcagta aaagtttttc tatgtttaaa
    10741 tagtgactca actctgtcaa tttatgccgt aaatcaaatt caggaaactg gtagatggtt
    10801 ttgtagagct tggcaaactg ctctctgctg ccaaatcctg ttaaataata aggatgtttc
    10861 atctgatttt taaaatagat ggcgtcaaaa tccttgtgaa caaactggtt gcgccaggtt
    10921 ttgaaatctt cagggatgtc attaatcaga atgactctgg catttgggtc ttcttcaata
    10981 gtcggaatac cttcaggaac tttggctgtt tttgtcctta agtcaagaag ttgcactccg
    11041 tcaacacgag catctactaa catgaattgg agactcgtgt tcccattcca atggttgaca
    11101 gataaggtaa ctgctaattc taaaccagta gcttgtcgga actcttgcag ttgacttcct
    11161 tgaccaaaag ccagtacgtc aaaggaagct ttgccctgac tgactttaaa tttaagatgg
    11221 ctctggtctt gccctatggt acgcgcttga ctaaccctta tgtccttgac ataaaagacc
    11281 ggcttttgat gatccatccc ataaggggct aatttatcta aactctttaa aatatcaagg
    11341 ctaaggtcgt ccaaagacag tctttcatca atagttaacg tatttttggc agtctgatct
    11401 aagccacgtt ctatcacaaa ctgacaaaga aagtctgaaa gagcttccaa gttgtcaacg
    11461 ggcagggtca ttcctgctgc accagcatgt cccccaaagg ctgtaaataa ctcacgcttg
    11521 ccattcaagg cttcaaaaat attaatagct tctaaacttc tggccgagcc ttttgcaaag
    11581 ccattatcaa tggtcaaaac gacgaccgtt tgtccaatag tttccatgat acgaccagcg
    11641 acaatcccaa gaacaccagg atgccagcca gcttgtgcca agacttgaac aggtttttga
    11701 ggatcaacca tagccatcgc ttgatcaaaa atgtcttgga ctaaggcctt acgctcttca
    11761 ttttttttct taatcatcaa ggcaatcgct tgagcttctt ggtcatcaaa gcccgtcagt
    11821 agctcaatgg ctggattagg gtcatctaag cgccctaaag catttaattg cggagcaatc
    11881 tgaaagccaa tggcgtcttc attaaaatgt tccaaatcaa taggagacaa ggacatcaac
    11941 tcctgcaagc ccactctttc ggtgtgtttg agcatggcca gaccattttt gactaaaaca
    12001 cggttttcac ctgtcaagct taccatatcg gcaatggttc caatagctac caagtctaga
    12061 cagtctgttg gtaaactttc tagcaaagct gttgccaatt taaaggcaac gccacagcca
    12121 gctaagtgtt taaagggata atcagcatca ggatgctcag gatgaatgat ggcaaaggct
    12181 tctggcaatt cttcaggcag gctatgatgg tcagttacaa tgacatcaac ttcctgttct
    12241 tgagcatatc taatcgcttc atgtccggcc acgccattat caaccgtgat aattagggac
    12301 acagcttcct gctcaataaa atatttatag acgctctggt tggggccata gccatctgta
    12361 aagcggttgg gaagataaac caaaggctct gctcccatca tgtctagggt ttctttgaca
    12421 atagaagccg acgtcatgcc atcagcatca tagtcgccgt aaatgagaat tcgttcgccc
    12481 tcttcaatag cttgacgaat tcttggaaca gcttttgcca tgtcatgtag caagtaagga
    12541 tcatgcagtt gagacaagtc cgctgttaaa aactcatcta aggcttcttc agtccttatc
    12601 cctcgatcat aaatcaactg agcggcggtc tgggttagac cttttgtctt tgctagctta
    12661 aagaagccat catctggctt cttgtctttt attttccagc tatatttgga tttaatcatt
    12721 tataattaaa tacctttctt gcaagataat cagataattt agggaaaagg gtgtaaaatt
    12781 gatgggtcac cgctaaacta aagggcaaat tcaattctcg tttatttttc ccgataatag
    12841 aaaccaaacg cttagccact tgatttggtt ggagagtaaa tgtaccaacg ctttccaaat
    12901 aatgtccaga cgggtcagct tggtcaaaaa acttggtggc aatgggacct ggattcacgg
    12961 tggtcacgta aaccccttta tccgctaatt ctaagcgaag ggcattggaa aatccgataa
    13021 gggcaaactt ggtggctgaa taaatgctcg atttggctga cgcaatcaag cctgccatgg
    13081 acacaatatt aataaggtga ccttgcccct gctctgccat tttctgacca atcaagcaag
    13141 caaagtgaat gctcgctagg gtgttaacct gaaacatatc agctatttct tgggcagaaa
    13201 actcttcaaa gcctttgaaa gctccgtagc cagcattatt aatcaagaca tcaatacggc
    13261 catagcgctg gtaaatctgg gcgaccattt tctcaatggc tactggattg gtaatatcca
    13321 actcaaggca ttctttgttg tcaatatgct gataacagtg ttctaggcgt tctttgttac
    13381 gtcctaataa aatcaagctg tcttccttgg gtaactgctt aacaattgcc tgagccagtc
    13441 ctccagaagc tcccgtgata acaatgattc tttgtgccat taaataatca cttcctctaa
    13501 atcttgtacc attttaacat tttcaaatat agtggcggca tctttttcca tctggcgaca
    13561 atcacgccct aaaaagcgag ctgatacatg attgagcaag aggcgttttg cgcctgcttc
    13621 atgagcgatt tgtgctgcct gcatgttggt agaatgcccg tgatttctcg caatccgctc
    13681 gtcaccctta ccataggtgg attcatgaac caaaacatct gcatctttag cgagtttaac
    13741 actggcagac gttttgcgcg tatcgccgat aatggtgatg attttgcctt ttttaggggc
    13801 agaaatataa tctttggcac aaatcaggcg gccgtcttcc aactcaacgt cttgaccatt
    13861 tttaattttg ccaaagagtg gtccaaaagg aacacccgct gcttttaagg cttctgcgtc
    13921 tagggttcct tctaagtctt tttgaactac tcgataaccc atgcaaaaaa tggtatgcgc
    13981 cagacgctct gcatagacct caaacttatc tgtttccatt attttaccta atgatttgtc
    14041 atcaaattca tgaaaatgaa tctgataagg tacacgcgct ccagaaactt ttagactagt
    14101 taacacgtaa gttttaatgc caattggtcc ataaatatca agatctgtct gttcttcact
    14161 agcttgaaaa gaccgacttg ataaaaatcc tggtaatcca aaaatgtggt ccccatgcaa
    14221 atgagtaatg aaaatctttc ttatttttcg tggcttaatc gtcgtttcta aaatttgtct
    14281 ttgcgttcct tcaccgcaat caaacatcca gacttcattg atttcgtcta gtaatttcaa
    14341 ggcaagactt gacacattgc gctgcttggc tggttgacca gctccagttc ctaaaaattg
    14401 taattccatt caatctattc tttctattgg gtgatgtaaa tcataaattc tcgttgggca
    14461 aaatcataat acttttgacc agaaaatgtt tggctaagag cccataactc ctctacatta
    14521 aaccccttaa gcatggtgat accgtttgcc agacggtaaa tcttcgacaa gagtgtgtca
    14581 tcaggcgtaa tgtcttcaca ttctccttgc tcaaactgct tagcctgcat tttaagctgg
    14641 cctttgtctt cataaaccat atattgtggg aaaatttgtg ttaattcaaa gaagagttcg
    14701 ggtttcttag gctctgcttt ttcagaaaat accaatgcaa cttcttggtc attttggtaa
    14761 gcaaaaccat aagcttttcc agacaaattg aggcgtacaa agggcttttc ttcctcaaaa
    14821 gatggttggt cagtaaaaag ccccaaagcc ttagaaagat taaaataata atcagtcgca
    14881 gcttgcggtg actgtttttg gtagatttca gcaaagtagg cattaataac acttggtggt
    14941 ggtgtgataa aagctagttt ttgctggtct gtcaaactgg ctaatagatg gtttaggtag
    15001 ttggtatcca gactagtaaa tccaccatag gtttttgcta aatcaatata attattcata
    15061 aaaatcgtca agtctccatt gctgttttgg ggaaatgtag cccgaaatat cctctatttc
    15121 ttggtcaaac gtgtagtggt ctaaaagagc aactcgattc aaatcataca gcttgtacgc
    15181 cttgtcttga tgcactttga ttctaaaagg aacaaactgg tctcgaattt ggtcaatgat
    15241 taaacgtctc aagagaattt tgctatcctt tgaacgggct gaaatcctga tatttggaaa
    15301 agcagtagct gtgaattgtt ctgctatatc caccttgtta taaatagcta aacgcggaat
    15361 attaagcatg tctagctctt ttagaaggtt taaaaccact ttttcttgct cactatgatt
    15421 ggggtcgcta gcgtcaatca catgtaacag caaatccaca tatttgcttt cttctagggt
    15481 tgatttaaaa gcagccacta attccgttgg caagtcttga ataaagccta cggtgtctgt
    15541 taaggttgct tgaaattgat tttctaaata gagttgcttt gtagttgcat ctaaggtagc
    15601 aaataattca ttggcttcat agtggctgtc atctgtcaac aagttcataa tggttgactt
    15661 gccagcattg gtgtagccaa tcaagccaat tttaaaggta tctgaaccca cacgacgttc
    15721 tctaattgtt tggcgatttt tctctacctg agttaattgg cgttcaatat cagcaatctg
    15781 atgacgaatc gaacggcgat ttaactctag ttggctttca cctggtccac ggctcccaat
    15841 accacctgct tgacgactta acatgatgcc ctgtccaacc aaacgaggta acatatattt
    15901 caattgggca aggtgtactt gtaatttgcc ctcatggctt ctggcacgca tggcaaaaat
    15961 atctaagatt aactgcatac ggtcaatgac ttttacctct aaaacagctt ctagattagc
    16021 attttgccta gcagtcaacc gattgttcac aataactgca tcaatctcat cagcttccac
    16081 aatcgcctta atctcatcca gtttccctga gccgataaag gttttgctgt cgtaacgttc
    16141 tcgtttttgg ctgaaacttg ccataacttt gacaccagct gttttggcaa gattagctaa
    16201 ttctgtcatg gacatgtcaa aatgttctgt tgtctgaagc tcaactccta aaagaatcac
    16261 tctttcttgc tgacgctttg tctcaatcat cacctttacc aatcctccaa attccgtaaa
    16321 ttctattata acatatcttc aacagattct gctaattttg cgtcatgact tctctttctg
    16381 accaagaaaa tccctgaccc tatcatgtac tacctgtggg taatcaggag ccgttatggc
    16441 agtgaagcta acagccattc gattgcggaa ccaagttagc tgtcgcttgg caaaacgtct
    16501 cgtgttttgt tttaattgat cactagcttc tgccaaagtc atttcaccta caaaataggg
    16561 gaataattcc ttataaccta tgccacgact agcctgaaca gtcgggtaat gctcatataa
    16621 ccattttgct tcttccaata agccgttttc aagcatacga ttcacgcgct gattgatacg
    16681 gtcatagatg acttggcggt catcattcaa gccgataata agcggctcat aagccgtctc
    16741 ggcattctct aattcatcag caaactgagc caattcaagg gctcggatcg cccgtctgcg
    16801 attcacctgc tcaatggtaa tgttattgac ttgcaagcgt tcatacaagt cgtgatcgtc
    16861 caattgctcc aactcatttc gataggcttt taccgcctct tgatcaactt gacctcctag
    16921 atgataacct tcgagtaaac tttgtaagta aagaccagtt ccacctacaa taattggtaa
    16981 tttcccacga ctaacaatgt ctgaaatagc tttttgagca tcttggacaa aatcgtaggc
    17041 cgaataagac tccgtcacct cacgaatatc aattaagtga tgaaccgctg cttcttgctc
    17101 ttcttgagtg gccttggctg ttccaatatc caactgtcga taaacttgct ggctatctcc
    17161 tgaaataatt tctccattaa aagccttggc taaactaatc ccaagtgctg tttttccaac
    17221 cgctgtcgga ccaacaatca caactatttt tatttttgtc attttcttgt ctttctatta
    17281 gaaaagcgtg tccaaaaacg ccttttactt gaaaattagc ttatttttcg ctaccatata
    17341 ttataacata agattaagga ccactgaggt ccttcactag aaatggagaa taatcatggt
    17401 taagaaatat caatttgtta aaggtcttgc tactggcgtc ctagcaactg ctgctactgt
    17461 tgctggtgct gtctttgctg taaaaaaaac aatcattgac cctgaagaag aaaaagctgc
    17521 tttcattgaa gaaaaccgca aaaaagctgc gcgtcgtcgt gtagctcgct aacgtgacaa
    17581 tggatcaatt taaaagagac tagcacatta aagtgccggc ctctttttta gtaatctcga
    17641 taactagtta gtagtaactg tcttttgatg agccaagata ttagctgctt tacttatttt
    17701 gaaatagaaa tctcaggtaa gccccataaa cataagtgac taaagctgtc gccaacaata
    17761 cctctcctag caaaagaacc tgccaaccta aaaaagataa ggtcattttt aaggaagtgg
    17821 cactaatcac aaacggaaag gtaaaagctg aaaaacctgg gttaaaggga cgattgagta
    17881 agcgaggaag ctgtacaacc acaaaagcat ataacagttg tgagctgatg agaagaaaga
    17941 taaccattcc tacttgtggt ctaggaaagg ttgctaaata agacgctgaa agtaaagaca
    18001 gaggggcaca aaacgttgaa atattaggca ttactgcttg acctagacca atcccataag
    18061 tctttttggc cataaaaggt aaaatcacag cagttagggg aagacaaacc caaaaaatca
    18121 cctgtcctaa taaaaactgc ctactagcag gtgctgttaa tgccgccatt gcaattccaa
    18181 caaaaagcac ggaccaggaa ggaaaaacgt tatcccatga aaaagaaaag acaaaacgat
    18241 actggtaata agcaatcagg acaagatttc ccaaaaagaa tagccaccaa gtgagaaagc
    18301 ctaaccaaca ccctcctgtc actttgagaa agagtgaaga caacaacacc cctgtcataa
    18361 aaaaagttgg aaaaacagag gcaattagag gttgtcgtaa ctgcattttg gtatcgttaa
    18421 ggttcctaac catgccaaca agcaagatac catagataaa aagagctgcc agaattccca
    18481 aataactaaa aatcgaaaca taagtagcca acaaattgcc aaatgacaac gttcctaatg
    18541 ccaatcctga catgaccaat ggtgggtttt taaggtgttt cataagtgtc cttttctgcc
    18601 tattaacagc tatttttagt gacataatct tgtttatttt atcacatttc gagcattttt
    18661 gccataaagg ctgccagatg cggataattc tcgtaagatt ttggtgtttt ttgagaccat
    18721 agcgccatgg cgtaagcata agcatctgca atggtttgtt gatctcgata aacatgccca
    18781 ctatccccaa taagaccatc taaatgcgtc atagcaatat ccatccgctt gtaagaagct
    18841 tgtttgactt tttcaagcgc ttgattgtct tgagaaatag tgtagcgatg agggttgtac
    18901 attggcctaa aaacaagggg aaaatcacct gttaagaaat ccatgatttc gttgaatcaa
    18961 ctttctgcca aaagattatc ttggggaatg agctgagatt tcagggtttg tttcgctata
    19021 aagagcaaga taatacctgc ctgtgtgacg atttgaccat catcaagctg taaagcttaa
    19081 accattccca aaggattgat ggtaaggcag tcttcagaat ccagttttac cttctctatc
    19141 tcgtaataca gaaactcaca attcttttga aaaggttcta gacagagttg gtgttgacgt
    19201 cttcgctgtt gatggctctg catgacaaaa tgagcttggt gtccacgttc actagtatct
    19261 tgttgcaaag tctttgcaat ttggtctcat catctgtatc acaaaaaaca aaccagtttc
    19321 ctgatttgtt ttgagatatt aggcacctac aaattctggc aaggattctc caatagcttc
    19381 taaacgttct gcccattctt gacgtgataa accattttct tgcacatagc gattacgttc
    19441 atgtgcacga cattcaggcg agcatcctcg aacatacttt gtttcgtttt cttctgaagc
    19501 aaaaatttgt ttgttacaaa acggatttgc acaattgaca taacgttcac aaggtgttcc
    19561 atcaaaataa tctttactaa tgacagttgg attaacatga ttaattggca ccgagatacg
    19621 atcatcaaaa acatacatcg caccatccca aagttcacct tgaacttccg gatcttttcc
    19681 atatgttgca ataccaccgt gtaattgtcc aacatcttta aagccttctc gaaccagcca
    19741 acccgagaat ttctcacagc gaacaccacc cgtacagtac acaacaacac gtttttccat
    19801 aaacttatct ttgttgtcac gtacccattg tggtaactca cggaaattgc gaatatcagg
    19861 acgaattgct ccacggaagt gacctaagtc atactcataa tcattacgcg tatctaaaac
    19921 aacagtatct tcatcaagca aggcttcttt aaattgttta ggatttaaat actcacctgt
    19981 tgtttccaaa ggattaatat cactatcaaa gttattgtct tctaacccaa ggtgcacgat
    20041 ctctttctta taacgaacaa acattttacg gaaagcttgt tgattttcct cgtcaatctt
    20101 gaaccaaaga tcagcaaaac gctcatcgct gtggacccaa tccatatact tctgagttgt
    20161 ttcataatct ccagaaacag ttccattaat cccctcatcc gcaatcaaaa tacgaccttt
    20221 aagcccaata gatttacaaa attctagatg tttcgcagcg tactcttgcg cattttcaat
    20281 tgaaacatat ttataataga gtaaaactct gattttttct gacatttttt ctccttcatt
    20341 gttattcagt tacttcctat tatatctagg aaaaacaagt ctaggcaagt catttgtttg
    20401 tctttttttt cacaaaatag atctcaattt atttttgatg agatgagatg caatccatta
    20461 ctcctttaag cagatcatta gaagctttat ttagaaaatg ctaagataaa acaaggaggc
    20521 caaaatgact tatgaacttt gtttagaata tgggacttat ccgttgtcac gtgtttatgc
    20581 ttattgggga gaagatcaaa atcccccaac attcattcaa gaagatcgac tgctttgcca
    20641 taaactagaa actatgaatc acctgttcca tgatttattt gtaactattg aatcacaatt
    20701 ccattatgtt ggttttaaca tgcctgaaaa aagagcgcag attcggatac tatatcaaga
    20761 agtagctact atattaaaat ccaaatataa ggattatcca attaagattg agacgtttct
    20821 tctttaaaaa tctaatacta aaaaactaac tgtgcttaga cagatgacac agctagtttt
    20881 ttaggtaaaa ctcttcttaa attttaaaac gtttcgaaaa atcactaaat gctttaatag
    20941 cacttgctaa aaatggtttc accatctcat caataattaa ccttgattac ttacaatctt
    21001 atgaatctga gtcaaataaa attcaggctg tggcattaca gagatattta aaacctatac
    21061 tcatcgtttt caaggtcaga aaaataatga ttttgaaaag gttcgtcaac tcttctagat
    21121 aattttggac aaattttgga aaagaaacaa aaaaagccta tcaaataggg cttttttata
    21181 tgatttctat ccaccttgag ggaatcgaac ccccatctca agaaccggaa tcttacgtga
    21241 tatccattac actaaaggtg gtcacaactt tgtcaatacc cttatagtat atcataaaat
    21301 gaaataatat gaaataactt ttctaaccag aagacaataa aaagttgagg aaaacctcaa
    21361 ctttttagat ttattacaat tcaatatcac cgaaaagatc agccattgag aatccagttt
    21421 gtgtttctgg gagttcgtag tcacgtcttg attcacgttt tggacgacgt gggcgtgatt
    21481 gacgtttttc ttctttgttg tctccttcag cttgcgctgg gcgttcttca agagctttga
    21541 ttgataatga tacgcgctca tcagctgcat tcacttcaag aactttaact gttacttctt
    21601 gtcctacaga aagtacatct tttggatttt caacacgttt gtgtgaaatt tgtgaaatat
    21661 gaacaagtcc atcaatacct ggtaatactt caacaaaagc accgaagtca gtcaagcgtt
    21721 ttactttacc ttcaacaaca tcaccttgag caagtttttg ttcaacgccg tcccatggtc
    21781 caggtgttgt tgctttaagt gaaagtgaca cacgaccagc ttcttcgtca attgaaagaa
    21841 ctttaacttc aacttcttca ccaactgaaa caactgattt aggtgacaca ttacgttcgt
    21901 gagacaattc agtcacgtga acaagtccgt caacaccacc aaggtcaatg aaagcaccaa
    21961 aacttgttaa acgcgctaca gtacctgtaa cgactgcacc ttctgaaatc ttagagaaca
    22021 cttcagcacg agcttcttta gcggcctctt cgataacttc acgacgtgat aggatgaaac
    22081 ggttttctgc tgcatcaact tctttgattt tagcatcaaa ctcttgtcca acaaattttt
    22141 cagtgttgcg aacaaaacga gtgtcaatca ttgaagcagg gataaatcca cgaagtcctt
    22201 caaattcaac tgaaagacca cctttaacag cacgggtacc tttaacagta acaacttcgc
    22261 cttcacgacc aacaagtttg tcccaggctt tacgagcttc caaacgtttt ttagatacta
    22321 ggaaagtaac tgtatcagta tctttaccta ctacttgacg aagaacaagt acttcaactg
    22381 tgtcgccagc tttaacaaag tcgttaatat cagcatcgcg gtcgttagtc aattcacgaa
    22441 gtgtcaagac accttcaaca cctgttcctt cgataacaac gtttgcttga ccgttatcaa
    22501 cagttaaaac ttccgcagtg acaacatcac cagggttcac ttcgctaaca ctgtttagca
    22561 aatcttcaaa ttcattcatt ctaaaaaatc ctccaacaaa agctagccaa tgctagcttt
    22621 tgataacaat atattttctc aaggtaaccc gcaacgacaa caactcttat ctttgacggt
    22681 ctcagggagc acctgatacc tct
//
LOCUS       DAUDYX010000034        21430 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.034, whole genome shotgun sequence.
ACCESSION   DAUDYX010000034 DAUDYX010000000
VERSION     DAUDYX010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21430
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.034"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            145..1128
                     /gene="guaC"
                     /locus_tag="VHD02_001254"
     CDS             145..1128
                     /gene="guaC"
                     /locus_tag="VHD02_001254"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEQ5497862.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIITSRSQADTSVTLGKYQFKLPVI
                     PANMQTIIDETIAEQLAKEGYFYIMHRFDEDSRKPFIKRMHEQGLIASISVGVKAYEY
                     EFVTSLKEDTPEFITIDIAHGHANSVIDMIKHIKTELPETFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHIESPGKTVEVNGETFKEYYGSASAYQKGEHKNVEGKKIL
                     LPTKGHLSDTLTEMQQDLQSSISYAGGKDLDSLRHVDYVIVKNSIWNGDSI"
     regulatory      1251..1346
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            1433..2014
                     /locus_tag="VHD02_001255"
     CDS             1433..2014
                     /locus_tag="VHD02_001255"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEQ5497863.1"
                     /translation="MQLLEERILTDGNILGENILKVDNFLTHQVDYRLMKAIGKVFAQ
                     KYAEAGITKVVTIEASGIAPAVYAAEAMDVPMIFAKKHKNITMTEGILTAEVYSFTKQ
                     VTSTVSIAGKFLSKEDKVLIIDDFLANGQAAKGLIEIIGQAGAQVVGVGIVIEKSFQD
                     GRRLIEDMGIEVTSLARIKNFENGNLNFLEADA"
     gene            2014..3296
                     /locus_tag="VHD02_001256"
                     /pseudo
     CDS             2014..3296
                     /locus_tag="VHD02_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984674.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
     gene            complement(3360..4298)
                     /locus_tag="VHD02_001257"
     CDS             complement(3360..4298)
                     /locus_tag="VHD02_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEQ5497864.1"
                     /translation="MFVTQQLKLMGTVIDIQIESDKACQQLSRVIDLLYTYKNRFSAN
                     DSNSELMAINQVAGVKPVSVHSDLFNLIQIGKAHSLSTPSNLNIAIGPLVQAWRIGFE
                     DARVPSHNLISQQLALTDPRQVLIDDKKQTVFLQQVGMALDLGALAKGYITDKIMAYL
                     IEDGIDSALINLGGNVRVHGPNPKSPDKTFRIGIQKPDAKRGQHLGVIKVNNHSVVTS
                     GIYERQFTSKGKQYHHILDRQTGCPIETDMLSLTIMAPSSFYCDIWATRLFGLDSSMI
                     ITLLNTFDNIEGLLVTRKHHVLMSNGLRHYFQPYYH"
     gene            complement(4351..4536)
                     /locus_tag="VHD02_001258"
     CDS             complement(4351..4536)
                     /locus_tag="VHD02_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEQ5497865.1"
                     /translation="MPFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGEMKQKS"
     gene            4674..5243
                     /locus_tag="VHD02_001259"
     CDS             4674..5243
                     /locus_tag="VHD02_001259"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEQ5497866.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     GFGIVSSRIGMRREAIPISNDMDIFTFIAQLEEKPYCVLIDESQFLSKQNVYDLARVV
                     DELNVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPEI"
     gene            5278..6357
                     /gene="prfA"
                     /locus_tag="VHD02_001260"
     CDS             5278..6357
                     /gene="prfA"
                     /locus_tag="VHD02_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEQ5497867.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQTISDAEEMIKDASGDPELEEMAKEELKESKAAKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DQKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTKKLESLNN"
     gene            6357..7196
                     /gene="prmC"
                     /locus_tag="VHD02_001261"
     CDS             6357..7196
                     /gene="prmC"
                     /locus_tag="VHD02_001261"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEQ5497868.1"
                     /translation="MNYATLIRTYEDKLEQIDEDRENLAYVFREIKEWSSLDMLIHQN
                     QAVTPEDAVLLEHIFCSLSQHLSPQYITGNAYFRDLKLAVDKRVLIPRPETEELVDMI
                     LAENLDAPLNVLDIGTGSGAIAISLKKERPNWQVTASDISRAALDLAKANADAYQLDI
                     TFIESDVFSLISGTFDIIVSNPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRK
                     IIEQADNYLTKEGKLYFEIGYKQAEGIKDMLQAYFPQRHIRVVTDIFGKERMVVVDYG
                     VSS"
     gene            7180..7770
                     /locus_tag="VHD02_001262"
     CDS             7180..7770
                     /locus_tag="VHD02_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEQ5497869.1"
                     /translation="MEYLASIIESGDALVLPTETVYGLFAKALDEKAVNAVYDLKQRP
                     RDKAMNLNVADFNSILVFSKEQPRYLKKLYQAFLPGPLTIILKANDQVPYWINSGLST
                     VGFRLPSHPITTALIQKTGPLIGPSANLSGKASGRVFDHIMQDFDFQVFGYADDPFLT
                     GKDSTILDLSGERAVILRQGAITKEELLANVPELRF"
     gene            7788..8240
                     /locus_tag="VHD02_001263"
     CDS             7788..8240
                     /locus_tag="VHD02_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ5497870.1"
                     /translation="MLRPLSKTDCPMLQEINAKALGYLVSLDLLERQYERLIEDCHHY
                     FLAYADKDTNQLLGYVHAERYETLYASDGLNLLGLAVLPAYQRRGIGSALLRALESQA
                     RQEGIAFIRLNSASHRKEAHAFYRNLDYADDKTQLRFIKNLQGDDNDF"
     gene            8230..9486
                     /locus_tag="VHD02_001264"
     CDS             8230..9486
                     /locus_tag="VHD02_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEQ5497871.1"
                     /translation="MIFDKGNVEDFDKELWDAIHAEEERQEHHIELIASENMVSKAVM
                     AAQGSVLTNKYAEGYPGNRYYGGTECVDIVETLAIERAKKLFGAAFANVQAHSGSQAN
                     AAAYMALIEAGDTVLGMDLAAGGHLTHGSPVNFSGKTYHFVGYSVDADTEMLNYEAIL
                     EQAKAVQPKLIVAGASAYSRSIDFEKFRAIADHVGAYLMVDMAHIAGLVAAGVHPSPV
                     HYAHIVTSTTHKTLRGPRGGLILTNDEALAKKINSAVFPGLQGGPLEHVIAAKAVAFK
                     EALDPAFKDYAQAIIDNTAAMAAVFAQDDRFRLISGGTDNHVFLVDVTKVIANGKLAQ
                     NLLDEVNITLNKNAIPFETLSPFKTSGIRIGCAAITSRGMGVKESQTIAHLIIKALVN
                     HDQETILEEVRQEVRQLTDAFPLYKK"
     gene            9493..10470
                     /locus_tag="VHD02_001265"
     CDS             9493..10470
                     /locus_tag="VHD02_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEQ5497872.1"
                     /translation="MLDSYIKRIVIHQFSPNDTELLLSDRLVSITPRIDEYFRKKLAK
                     VFSDEAKRGQFEANNTFFTTIGDDLLETSVTIAQLWKEAFVISEDQKTNDLVFVQFDK
                     DGEPFFAFLRIALKEQFAHLSDNYEHPFTVTQNNLPSPTQTPDEALVINLKSGQYYLI
                     EKRVKHNGSFANYFSEHLLKVTPEQSVKKSIKMIEQTAQKIAEHFNQDDFTFQSKMKS
                     TLFKQLEADDVLSPEKLADQLFDDNLTARLTFVDQVKDVIPEPIKISDIDHSRQIKKL
                     ENQKLSLSNGIELTVPNAIYQDAEAVEFLLNDDGTYSILIKNIEDIKTK"
     gene            10471..11070
                     /locus_tag="VHD02_001266"
     CDS             10471..11070
                     /locus_tag="VHD02_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEQ5497873.1"
                     /translation="MFRLLKRACSFLLLFVIYQSFVIHHNVQRVLAYKPMVEKTLAEN
                     DTKANVDLVLAMIYTETKGGEADVMQSSESSSGQKNSITDSQASIEHGVNLLSHNLAL
                     AEEAGVDSWTAVQAYNFGTAYIDYIAKHGGQNTVDLATTYSKTVVAPSLGNTSGQTYF
                     YYHPLALISGGKLYKNGGNIYYSREVHFNLYLIELMSLF"
     gene            11080..12798
                     /locus_tag="VHD02_001267"
     CDS             11080..12798
                     /locus_tag="VHD02_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5497874.1"
                     /translation="MKRLRPYLKGYLKESILGPLFKLLEALFELLVPLLIANMIDISI
                     SQHNSQGILRVVLTLFGLATIGLLLSVTAQYFSSKAAVGFTRQMTDDLFKKIMFLSKE
                     DQDHLGYASLVSRLTSDSFQIQTGINQFLRLFLRAPIIVCGAMVMAYWISPSLTLWFV
                     MMVIVLLTLVFVMSHLLGPLYLLIRRETDHLVRLTSQQLQGIRVIKAFNQTQKELQAF
                     KQQNMLLSRHQYQAATLANVLNPMTFLVVNLTLLILIWQGSWQVAHRSLSQGMLVALI
                     NYLLQILAELLKMTMLMGTINQSVTAAKRINQVFVLADEVPLPLLKDGPISTHLLTIR
                     HLTFTYPGAAEPSLYDIQLSADQGEWIGIIGGTGAGKTTLIDLICQTYSQYSGEISLN
                     WQGEVPKTLTEWRNVIALVPQKAQLFKGTIRSNLLLGQSMPINDEELWRALELAQAKE
                     FVAALPEQLEAPVEAFGRHFSGGQRQRLAIARALLKPKPILILDDASSALDNETRGRL
                     FKALKEELSDVLVILVTQSIKNLQFADKILVLEQGHQLDFASHDQLKVSNALYQEMLA
                     LYVKGE"
     gene            12801..14528
                     /locus_tag="VHD02_001268"
     CDS             12801..14528
                     /locus_tag="VHD02_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5497875.1"
                     /translation="MIKTDHHLLKRVLQDLLKKPLPVCILVIASFVQVGLSVYLPVLI
                     GKAVDMSLSVNSWQTLKWLLGQMLVVIVVNTLIQWVMPLVYSRLLYQYSQQLKDKLLE
                     KIHRLPFAYLDRQTIGDLVSRVITDTEQLINGLQLVFNQFILGLLTILCTIIAMAQID
                     WLMLILVLVLTPSSLFLARFIAQKSFHYAQAQTKSRGNLAQFTEEILRQEGLVQLFNA
                     QEQSICDYHVLNKTYCEASQKAIFYASTVNPATRFINSVIYALLAGLGAVRIMAGLFS
                     VGQLTTFLNVVVQYTKPFNDISSVLAEIQSSLACAQRLYDLLDIEIKEQEHFLTFKAS
                     AVKGQIDFEEVSFSYQKDRPLLKDINFSVPAGSKVAIVGPTGAGKSTLINLLMRFYEL
                     DAGSIKLDKVPIKCYAKEELRSITGMVLQETWLKDATVHELIAYGSEEASRGEVVAAA
                     KAAHAHFFIMQLPKTYDTYLSASDDALSQGQLQLLAIARMFLKKPKVLILDEATSSID
                     IRTEAVIQEALKELMRGRTSFIIAHRLSTIQSADLILVMDQGRLVEWGTHASLMSKNG
                     CYARLQKIE"
     gene            14769..16139
                     /locus_tag="VHD02_001269"
     CDS             14769..16139
                     /locus_tag="VHD02_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEQ5497876.1"
                     /translation="MSKIVVVGANHAGTACIKTMLTNYGDANEIVVFDQNSNISFLGC
                     GMALWIGEQIAGPEGLFYSDKEELESLGAKVYMESPVQSIDYDAKTVTALVDGKNHVE
                     TYDKLIFATGSQPILPPIKGAEIKEGSLEFEATLENLQFVKLYQNSADVIAKLENKDI
                     KRVAVVGAGYIGVELAEAFQRKGKEVVLIDVVDTCLAGYYDRDLTDLMAKNMEEHGIQ
                     LAFGETVKEVAGNGKVEKIITDKNEYDVDMVILAVGFRPNTTLGNGKIDLFRNGAFLV
                     NKRQETSIPGVYAIGDCATIYDNATRDTNYIALASNAVRTGIVAAHNACGTDLEGIGV
                     QGSNGISIYGLHMVSTGLTLEKAKRLGFDAAVTEYTDNQKPEFIEHGNFPVTIKIVYD
                     KDSRRILGAQMAAREDVSMGIHMFSLAIQEGVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(16298..17281)
                     /locus_tag="VHD02_001270"
     CDS             complement(16298..17281)
                     /locus_tag="VHD02_001270"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEQ5497877.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVTQNIAQELGIIDIFKEK
                     TQGDAEDLSHALAFTSPKKIYAADYSDCHDADLVVLTAGAPQKPGETRLDLVEKNLRI
                     NKEVVTQIVASGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQAL
                     AAKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVGLYDWLQANRDIDEQGLVDLFIS
                     VRDAAYSIINKKGATFYGIAVALARITKAILDDENAVLPLSVFQEGQYEGVEDCYIGQ
                     PAIVGAYGIVRPVNIPLNDAELQKMQASANQLKAIIDEAFAKEEFASAAKN"
     gene            17472..19958
                     /gene="gyrA"
                     /locus_tag="VHD02_001271"
     CDS             17472..19958
                     /gene="gyrA"
                     /locus_tag="VHD02_001271"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEQ5497878.1"
                     /translation="MQDRNLIDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALELLRDINKNTVNFQDNYDGSEREPVVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLAESIDAVKMVMEHPDCTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYDTGKGSIVLRSRTEIETTQTGRERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRLEGITAVRDESSREGVRFVIEIRREASATVILNNLFKLTSLQTNFSFNMLAI
                     ENGVPKILSLRQIIDNYISHQKEVIIRRTQFDKDKAEARAHILEGLLIALDHLDEVIA
                     IIRNSETDVIAQTELMSRFDLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LSDTLAKPERIITIIKEEMDEIKRKYANPRRTELMVGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGVQGTGVNNDDFVRELVSTSTHDTLLFFTNFGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLEDGETIQTIINARKEETAGKSFFFTTKQGIVKRTE
                     VSEFNNIRQNGLRALKLKEGDQLINVLLTSGQDDIIIGTHSGYSVRFNEASIRNMGRS
                     ATGVRGVKLREDDRVVGASRIQDNQEVLVITENGFGKRTSATDYPTKGRGGKGIKTAN
                     ITPKNGQLAGLVTVDGTEDIMVITNKGVIIRTNVANISQTGRATLGVKIMKLDADAKI
                     VTFTLVQPEDSSIDEINTDRENSISKNKDN"
     gene            19978..20727
                     /locus_tag="VHD02_001272"
     CDS             19978..20727
                     /locus_tag="VHD02_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEQ5497879.1"
                     /translation="MVKKQKRRKIKSMSWARKLLIVVLLILGLALLFNKPIRNTLIAR
                     NSNKYQVTKVSKKQIKKNKEAKSTFDFQAVEPVSTESVLQAQMAAQQLPVIGGIAIPE
                     LGINLPIFKGLGNTELIYGAGTMKEEQVMGGENNYSLASHHIFGITGSSQMLFSPLEK
                     AQNGMSIYLTDKEKIYEYIIKDVFTVAPERVDVIDDTAGLKEVTLVTCTDIEATERII
                     VKGELKTEYDFDKAPADVLKAFNHSYNQVST"
     gene            20810..21223
                     /locus_tag="VHD02_001273"
     CDS             20810..21223
                     /locus_tag="VHD02_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEQ5497880.1"
                     /translation="MKLNAIHHVAIIVSDYHLSKDFYVNKLGFEIIRENYRPDKHDYK
                     LDLSCGRIELEIFGKVTSDPYYQAPPKRVSQPEFKSEACGLRHLAFRVTNIESYVDDL
                     KSLGIPVEPIRHDDYTGEKMTFFFDPDGLPLELHE"
BASE COUNT         6655 a         3754 c         4418 g         6603 t
ORIGIN      
        1 atctataaaa tgaaaggtaa gtttttacct aaagatacct ctaatcatca gaggtcatag
       61 gaagattatt ttgtatagag gacttagaga agaaggtgag aactaacttg tttttcttaa
      121 tctatgttaa aagaggagaa acttatgttt aatgacatcc ctgtatttga ttatgaagat
      181 attcaactga ttcctaacaa gtgcattatt actagtcgtt cacaagcaga tacaagtgtg
      241 acactcggaa aataccagtt caagctacca gttatcccag caaatatgca aaccattatt
      301 gatgaaacaa ttgctgagca gttggcaaaa gagggttatt tttatattat gcatcgtttt
      361 gatgaagata gccgcaaacc ttttatcaaa cgcatgcatg aacaaggctt gattgcatcc
      421 atctctgttg gggttaaagc ttacgaatat gaatttgtga cttctttgaa agaggatact
      481 cctgagttta tcaccattga tattgctcat ggtcatgcta acagtgttat tgatatgatt
      541 aagcatatca aaactgaatt accagaaaca tttgtcattg ctggaaatgt tggtacgcca
      601 gaagctgtac gagaactcga aaatgcaggt gctgatgcta ctaaagtagg catcggtcca
      661 ggcaaagtat gtatcactaa agttaaaaca ggtttcggta caggtggttg gcagttagca
      721 gcactgcgct ggtgcgcaaa ggcagctcgt aagcctatca ttgctgatgg tgggattcgc
      781 acacatggtg atatcgctaa atccatccgc tttggagcta gtatggtcat gattggttcg
      841 ctttttgcag ggcatattga aagtcctgga aaaacagtag aagtgaatgg tgaaacgttt
      901 aaagaatatt atggctcggc gtcagcatat caaaaaggcg agcataaaaa tgtagaaggc
      961 aaaaaaatct tattgcctac aaaaggtcac ttatcagata ccctgaccga aatgcaacaa
     1021 gatttgcaat cgtccatttc ttacgcgggt ggtaaagatt tagacagttt gcgtcatgtt
     1081 gattatgtta tcgtcaaaaa ttctatttgg aatggtgatt ccatttaaat gatatggagt
     1141 tagctaggac cagtaaagca ttttttagct taatgctgga gatgctttta tcatatctct
     1201 gtaggtattg aaaaaaataa gaaacatggt aaaatagtaa attatcataa tgaattcaat
     1261 aatgacatac ttatttatgc tgtgaattgg cgcagcgtct ctacaagaca ccttaatgtc
     1321 taacaataag taagctttta ggcttgcttg gtttttaacc ttgcaaagtg agaaactctt
     1381 atagtttctg gctttgcggc aggctttttc tatttgaatc aaaaggagat ttatgcagtt
     1441 acttgaagaa cgtatcttaa cagatggcaa tattctagga gagaatattc taaaggtaga
     1501 taatttttta actcatcaag ttgattaccg gttgatgaaa gcaattggta aagtgtttgc
     1561 tcaaaaatat gctgaggctg gcattacaaa agtggttaca atcgaagctt caggtattgc
     1621 accagccgta tacgctgcag aagcaatgga tgttcctatg atttttgcga aaaaacataa
     1681 aaacattacc atgacagaag gcattttgac agcagaagtt tattctttca ctaaacaagt
     1741 gacgagcacg gtgtctatcg ctggtaaatt cctatctaaa gaagacaagg ttttgattat
     1801 tgatgacttt ttagctaatg gtcaggcagc caaaggcttg attgagatta ttggtcaagc
     1861 aggggcacaa gtcgtcggcg ttggtattgt gattgagaaa tctttccaag atggtcgtcg
     1921 attgattgaa gatatgggca ttgaagtgac ctcattagcc cgtattaaaa actttgaaaa
     1981 tggtaatcta aacttcttgg aggctgacgc ataatgacac attcaacaaa gcaggaacat
     2041 tctcactcgc aatcagctgt ccttggctta cagcatgtgc tttcgatgta tgccggatct
     2101 attttagtac caattatgat cgcaggtgct ttaggttatt ccgctagaga gttgacttac
     2161 ctgatttcga cagatatttt catgtgtggt gtggctactt ttttacagtt aaagttaacc
     2221 aagcatacag gagtagggtt accagttgtt ttaggctgtg cttttcaatc agtggcacct
     2281 ctatccatta ttggagcgca acaaggttca agtgccatgt ttggagccct cattgcgtca
     2341 ggaatttatg tcattttagt ggcaggtatt ttttctaaga ttgctcgttt cttcccacca
     2401 attgttacag gatcggtcat taccgttatt ggcttaagtc tcgtaggtgt tgctatgggg
     2461 aatatgggag ataacgtcaa agagcctacg gcacaaagca tgatgttatc attgttgacg
     2521 attgtcatta ttcttttagt ccaaaaattt accaaaggtt ttgtgaaatc catttccatt
     2581 ttgattgggc ttgtagcggg aactttggtt tcagccatga tgggattagt agatacaact
     2641 cctgttgtag aggcttcttg gattcatgtg ccaaccccat tttattttgg catgccaact
     2701 tttgaaatca cttctattgt gatgatgtgt atcatcgcaa cggtttctat ggttgaatca
     2761 acaggtgtct acttagcgct ttcagatttg actaatgatc aattagatga aaaacgtttg
     2821 cgcaatggtt atcgctcaga agggatcgca gtctttctcg gtgggttatt taataccttc
     2881 ccttacactg gtttttctca aaatgttgga cttgtccaaa tctcaggtat taaaacccgt
     2941 cgtccgattt actacgcagc aggtatcctt gtgatcattg gcctactccc taaatttgga
     3001 gaatggcaca aatgattcca agcccagttc ttggtggagc tatgcttgtt ctctttggta
     3061 tggttgctct tcaaggaatg caaatgctta atcgtgttga tttccaaaag aacgagtata
     3121 attttattat tgctgccgtt tccatctcag caggtttagg ctttaatggc actaatcttt
     3181 ttgccagctt accagagaca gctcaaatgt tcttaacgaa tggtattgtg atcgcaacct
     3241 tgacatctgt tgtcttaaat ttagttttaa atggcaaaga taaacaagat gaataattaa
     3301 aaaacggcta attgagccgt tttttctgtg atcagttttg agcaaaccgt taaccccttt
     3361 taatgatagt atggttgaaa atagtgtctc aaaccatttg acataaggac atggtgcttt
     3421 cttgttacta gaagaccttc aatattatca aaggtgttta aaagtgtgat aatcattgag
     3481 ctatcaagac caaataaacg ggtagcccat atgtcacaat aaaaagaaga aggagccatg
     3541 atcgttaagc taagcatatc tgtttctata gggcagcctg tctgacgatc taagatatga
     3601 tgatactgtt tccctttgga tgtaaactgc ctttcataaa ttccagaagt cacaacagag
     3661 tgattgttaa ccttgatgac accaagatgt tgcccacgtt tagcatctgg tttttgaata
     3721 ccaattcgaa aagtcttgtc agggcttttc ggattaggtc catgaacgcg tacatttcca
     3781 cctaaattaa taagggcaga gtcaatacca tcttcaatca ggtaggccat aatcttatca
     3841 gtaatataac cttttgctaa ggctcctaag tctaaagcca ttcctacttg ttgtaaaaag
     3901 acagtctgtt ttttgtcatc tataagaacc tgtctaggat ctgttagagc taactgttgt
     3961 gagataaggt tatgactagg cactctagca tcttcaaatc caattcgcca ggcttgaaca
     4021 agtggcccaa tggcgatatt aagattactt ggtgttgaca agctatgagc tttaccaatt
     4081 tggattaggt taaataaatc agagtgaacg ctgacgggtt taacaccagc cacctgatta
     4141 atagccatca attcagagtt tgaatcatta gctgaaaaac gatttttgta agtatataat
     4201 aagtcaatga ctctacttag ttgttggcaa gctttgtcag attcgatctg aatatcaata
     4261 actgttccca ttagctttaa ttgttgcgta acaaacataa ttagtcctct ttttagcaga
     4321 atacccccac taaggagtgg gggaaggtca ttatgatttt tgtttcattt caccttgagg
     4381 atagtaagtt ccttcaggca tatcgttgat aaaaacatgg atattttctt taggtgcttt
     4441 tgcaatgcgt gagactactt cggtaacctc gcgagctaat tgattttttt gttcttgtga
     4501 gcgtccctca aacaagtcaa ttgttacaaa tggcataatg ttactccttt atgttattgg
     4561 gcatcattgt aacataatgc tgtgaaaaag tatacactgt tttcaatggc tgggaaaact
     4621 tcagcctttg tggtaaaata ggtagaatac tatagaaagt aggattttcc gctttggctc
     4681 aactctatta taaatatggg acaatgaatt ctggtaaaac cattgaaatt ttaaaagttg
     4741 ctcataacta tgaagaacaa ggcaaacctg ttgtcattat gacaagtgct cttgatacac
     4801 gtgatggttt tggcatcgtc tcaagccgta ttggtatgcg acgagaagct atccccattt
     4861 caaatgacat ggatattttc acatttattg cccaattgga ggaaaaacct tattgtgtcc
     4921 tgattgacga aagccaattt ttaagcaagc aaaatgttta tgatttggcg cgtgtggtgg
     4981 atgagctaaa tgttcctgtc atggcttttg gtttgaaaaa tgatttccaa aacaatcttt
     5041 ttgaaggttc aaaacatttg ctattattgg ccgataagat tgatgaaatc aaaacaattt
     5101 gccaatactg tagtaagaaa gcaaccatgg ttttaaggac agaaaatgga aaacctgtct
     5161 atgaaggtga tcagatacaa attggtggta atgaaacata tatcccagtt tgtcgaaaac
     5221 attattttaa tccagagatt taaaaggtaa tcggttttaa aggagataca aagaaaaatg
     5281 aatatttatg atcaattgca ggcagttgaa gatcgttatg aagaattagg tgagttactt
     5341 agtgatccag atgttgtttc agatacaaag cgatttatgg aattatcacg tgaagaagct
     5401 aatactcgag aaactgtcac agcttataga gaatataaac aagtcattca aaccatttca
     5461 gatgctgaag aaatgatcaa agatgccagt ggtgatcctg aattagaaga aatggctaaa
     5521 gaagaactta aagaatcaaa agcagctaaa gaagagtatg aagaaaaatt aaaaatcctt
     5581 ctattaccta aagatcctaa cgatgataaa aacattattc tagaaattcg tggtgctgct
     5641 ggtggcgatg aagcagcgct ttttgctggt gatcttttaa caatgtatca aaaatatgct
     5701 gaaactcaag gatggcgttt tgaagttatg gaatcatccg ttaatggtgt tggtggtatt
     5761 aaagaagtgg ttgccatggt ttctggtcag tcagtttatt ctaagcttaa atatgaatca
     5821 ggagctcatc gtgttcaacg tgtcccggtg acagagagtc agggtcgtgt tcatacgtca
     5881 actgcaactg ttttggtaat gccagaagtt gaagaagtag aatacgacat tgatcaaaaa
     5941 gaccttcgcg tcgatattta ccacgcgtct ggtgctggtg gtcagaatgt caataaagtt
     6001 gctactgcag ttcgtatggt acacattcct acaggaatca aagttgaaat gcaagaagaa
     6061 cgtactcagc aaaaaaaccg tgataaagcg atgaaaatca ttcgtgctcg tgttgctgac
     6121 cattttgcgc aaattgctca agatgaacaa gatgctgaac gtaaatcaac agttggtact
     6181 ggtgatcgtt cagaacgtat tcgtacttat aatttcccac aaaatcgtgt aacagatcat
     6241 cgtattggtt tgacacttca aaaattagat actattttat ctggaaaaat ggatgaagtt
     6301 attgatgcac tcgttatgta tgatcagaca aagaaattag aatctctcaa taactaatga
     6361 actatgctac attaatcaga acttatgaag ataaactaga acaaatagat gaggatcgcg
     6421 aaaatctcgc ctatgttttt agagaaatca aagaatggtc aagtctagat atgttgattc
     6481 accaaaatca agctgtgact cctgaagatg ctgttctttt ggaacatatt ttctgttctc
     6541 taagtcagca tttatctcct caatatatta caggtaatgc ttattttcgt gatttgaagc
     6601 ttgcagtcga caagcgtgta ttaattccaa ggccagaaac agaagaatta gttgacatga
     6661 ttttagctga aaatcttgat gcaccgttga atgttttaga tattggtaca ggtagtggag
     6721 cgattgctat ttctctcaaa aaagaaaggc ctaactggca agtaacggca tctgatattt
     6781 ctagagctgc tcttgacttg gcaaaagcca atgctgatgc ttaccagctt gatataacgt
     6841 ttattgaatc agatgttttt agtctcattt caggaacatt tgatattatt gtgtcaaatc
     6901 ctccctacat atcctacgag gataaggaag aagtcagtct aaatgttttg caatcagagc
     6961 ctcatttggc actttttgct aaagaaaatg gctatgctat ttaccgtaag attattgaac
     7021 aagctgacaa ttacttaact aaggagggga agctttactt tgaaattggt tataagcaag
     7081 ctgaaggtat aaaagatatg ttacaagcct attttcctca aagacatata cgagtcgtaa
     7141 cggatatatt tggcaaagaa agaatggtgg ttgttgatta tggagtatct agctagtatc
     7201 attgaatcag gtgatgccct tgttttgcca acagaaacgg tttatggtct ttttgccaag
     7261 gctttagatg aaaaagcagt aaatgctgtt tatgacttaa aacaaagacc ccgagataaa
     7321 gcaatgaacc ttaatgtggc tgattttaat agtatcttag tcttttcaaa agaacagccg
     7381 agatacctca agaaattata ccaagccttt ttaccaggtc cgttaacgat tattttaaaa
     7441 gctaatgacc aggttcctta ttggattaat tcgggtcttt caacggttgg ttttcgctta
     7501 cccagtcatc caattaccac agctttaatt caaaaaacag gccctttaat tggtccttct
     7561 gcaaacttat ctggcaaagc tagtggtcga gtttttgatc atattatgca agattttgac
     7621 tttcaagtgt ttggttatgc agatgatcct tttctaacag gtaaggattc aaccatctta
     7681 gacttatcag gtgagcgtgc tgtgatttta cgtcaaggtg ctatcaccaa agaagaatta
     7741 ttagccaacg tgcctgaact tcgcttttaa gagaggagcc gcttggaatg ttgagaccat
     7801 taagtaaaac ggactgtcct atgctacaag aaattaacgc caaagccctt ggctatttag
     7861 tttcgcttga cttgttagaa aggcaatatg aacgattgat tgaggattgc catcactatt
     7921 ttttagctta tgccgacaag gataccaatc aattattagg ctatgttcac gctgaacggt
     7981 atgagaccct ttatgcttct gatggtctta atctcttggg cttggctgtt ttaccagcct
     8041 atcagagaag gggaattggg agtgccttac taagggccct ggaatcccaa gctaggcagg
     8101 aaggcattgc ctttattcgc ctcaattctg ccagtcatcg taaagaagcg catgcttttt
     8161 accgaaacct cgactatgca gatgataaaa cacaattacg attcatcaaa aacttacaag
     8221 gagatgacaa tgatttttga taaaggtaat gttgaagact ttgataaaga gctttgggat
     8281 gccattcatg ctgaagaaga aagacaagaa catcatatcg aattgattgc atctgaaaac
     8341 atggtttcaa aagctgtcat ggcagctcaa ggttctgttt taaccaataa gtatgcagaa
     8401 ggctatccag gtaatcgcta ttatggtgga acagaatgtg tggatattgt cgaaacgctt
     8461 gctattgaac gtgctaagaa acttttcggt gccgcatttg ccaatgttca agcccattcc
     8521 ggaagtcaag ccaatgcagc agcttatatg gccttgattg aggctggtga caccgtatta
     8581 ggaatggatt tggctgcagg tggtcatctc acgcatggct ctccagtaaa tttttctgga
     8641 aaaacctacc attttgtggg ttactcagta gatgctgata ctgaaatgtt aaattatgag
     8701 gcgattttag aacaagcaaa ggcggtccag ccaaagctta tcgttgcagg ggcttcggcc
     8761 tactctagaa gcattgattt tgaaaagttt cgtgccattg ctgatcacgt aggtgcttac
     8821 ttaatggtgg acatggctca catcgctggc cttgtagcag caggtgtgca cccgagtcct
     8881 gtccattatg cgcatattgt gacatcaaca acccataaaa ccttacgtgg tcctcgtgga
     8941 ggcttgatat taaccaatga tgaagctctt gctaaaaaga ttaattcggc cgttttccct
     9001 ggtttacaag gaggacctct ggaacacgtg attgctgcca aagcggttgc cttcaaagaa
     9061 gccttggatc cagcctttaa agactatgca caagctatta ttgataatac tgcggctatg
     9121 gcagcagttt ttgcacaaga tgatcgtttc cgtctgatct caggtggtac agataatcat
     9181 gtctttttag tagatgtcac taaggttatt gctaatggta aactagcgca aaatcttctg
     9241 gatgaggtta atattactct aaataaaaat gccattcctt ttgaaacctt atctcctttt
     9301 aaaacatcag gcattcgtat tggttgtgca gctataacca gtcgtggcat gggagttaaa
     9361 gaaagccaaa ctatcgctca cctcatcatc aaagccttgg ttaatcatga tcaagaaact
     9421 attcttgaag aggttcgtca ggaagtgcgt cagttaaccg atgccttccc actctataaa
     9481 aagtaaggaa tcatgttaga tagttatata aaacgtattg tcattcatca attttctccc
     9541 aatgacactg agttactgtt atcagatcgt ttagtgagca ttacaccacg aattgatgaa
     9601 tatttccgga aaaaattggc taaagtgttc tcggatgaag ccaaacgggg tcagtttgag
     9661 gctaataata ccttttttac aaccattggt gatgatttgt tggaaacgtc tgttaccatt
     9721 gcccagttat ggaaggaagc ctttgttatt tcagaggatc aaaaaaccaa tgatttagtc
     9781 tttgtccagt ttgataagga tggcgaaccc ttttttgctt tcttgcgaat tgctttgaaa
     9841 gaacagtttg ctcacctttc tgacaattat gaacatccat ttacggtgac gcagaataat
     9901 ctgccgagtc ccacccagac accagatgaa gcactagtca ttaatctcaa atctggtcag
     9961 tactacttga ttgaaaagcg tgtgaagcat aatggcagtt ttgctaatta tttttcagaa
    10021 cacctcttga aagttacacc tgagcaatct gttaaaaagt ctatcaaaat gatagaacaa
    10081 accgctcaaa aaattgcaga gcattttaat caagatgatt ttacttttca gtcaaagatg
    10141 aaatccactc tttttaagca gttagaagca gacgacgttt tatcaccaga aaaattagct
    10201 gaccaactct ttgatgataa tttaacagcc cgtttaacct ttgtggatca agtcaaagat
    10261 gtcattccag aacctataaa aattagcgat attgatcatt ctagacagat aaaaaaacta
    10321 gaaaatcaaa agctatcgtt atcaaacggg atagaattaa cagtaccaaa tgccatttat
    10381 caggatgctg aagcggttga atttttgtta aatgatgatg ggacttattc aatcttgatt
    10441 aaaaatatag aggatattaa aaccaaatag atgtttagac ttttaaagcg tgcctgcagt
    10501 tttcttcttc tttttgtcat ttatcaatcc tttgttattc atcacaatgt tcaacgtgtt
    10561 ttggcatata agcctatggt tgagaaaaca ttagcagaaa atgatacaaa ggcaaatgta
    10621 gacttagtct tagctatgat atacaccgag acaaaaggag gagaagcaga tgtcatgcaa
    10681 tccagtgaaa gcagttcagg acaaaaaaac tcgattacag atagtcaggc cagtattgag
    10741 cacggggtga atttactgtc tcataatctt gctctagctg aggaagcagg agtagattct
    10801 tggacggctg ttcaggccta taattttgga acagcttata ttgattatat tgctaaacat
    10861 ggaggtcaaa ataccgttga tttagcgact acctattcca aaaccgttgt ggcgccaagc
    10921 ctaggcaaca ctagtggtca aacctatttt tattaccacc ccctcgcctt gatttctgga
    10981 ggtaagcttt ataagaatgg cggcaatatc tattactcac gagaagttca tttcaatctt
    11041 tatttaattg aactgatgag ccttttttag aaagttatca tgaaacgatt acgtccatat
    11101 ttgaaagggt acctaaaaga aagtatctta ggtcctcttt ttaaattatt agaagcttta
    11161 tttgaattat tagtcccttt gttaattgct aacatgattg atatatcgat tagtcaacac
    11221 aacagccagg gaattttgag ggttgtttta acattatttg gtttagcaac cattggctta
    11281 ttgctttccg ttacagccca gtatttttct tcgaaagcag ctgttggttt tacaagacaa
    11341 atgacagatg atttgtttaa aaaaatcatg tttttgagca aggaggacca agaccatctt
    11401 ggttatgcta gtctggtatc acgattgacc agtgatagtt ttcaaattca aactggtatc
    11461 aatcaatttt tgcgtctttt tctaagggcc cctattatcg tatgtggtgc tatggtaatg
    11521 gcttattgga ttagtccaag tctaacccta tggtttgtga tgatggtgat tgtcttacta
    11581 acacttgttt ttgttatgtc acatttatta ggtccccttt atctcttaat ccgccgagaa
    11641 acagatcact tggttcgttt gactagccaa cagttacaag gaatccgagt aattaaggct
    11701 tttaatcaaa cccaaaaaga actccaagca tttaagcagc aaaatatgct cttaagtcgc
    11761 caccaatatc aagctgctac cctagccaat gtcttgaatc ctatgacttt tttagtggtc
    11821 aatcttactc tgttaatctt gatttggcaa ggaagttggc aagtagctca tagatcgctt
    11881 agtcagggca tgttggttgc tttaattaat tacctcttgc agattttggc agaacttttg
    11941 aaaatgacca tgttgatggg aaccatcaat caaagtgtaa cagccgcaaa acggattaat
    12001 caggtgtttg ttttagctga tgaagtgcct ttgcccttac ttaaagatgg tcctatttca
    12061 actcatctgt tgaccatccg gcacttgacc tttacttatc caggagcagc agagccaagt
    12121 ctgtatgata ttcagttaag tgctgatcag ggagaatgga ttggcatcat tggtggtact
    12181 ggagcaggca aaacgacttt gattgacttg atctgccaaa cttattcaca atatagtggt
    12241 gagattagtc ttaattggca aggagaagtg ccaaaaacac taaccgagtg gcgaaatgtg
    12301 attgctcttg ttcctcaaaa agctcaatta tttaaaggaa cgattagaag taacctccta
    12361 ctgggacaat caatgcctat taatgatgag gagctgtggc gggctcttga attagctcaa
    12421 gcaaaagagt ttgtcgctgc cttaccagaa caattagagg cacctgttga agcctttggc
    12481 cgtcattttt caggagggca acggcaacgt ctagctattg ccagagcact tctgaagcca
    12541 aaacccattt tgattttgga tgatgccagt tccgccttag acaatgagac tcgaggacga
    12601 ctttttaaag ctctaaaaga agagttatca gatgttttag tgattctagt cacgcaatcc
    12661 attaagaatt tgcagtttgc tgataagatt ttagtgttgg aacagggcca tcaattggat
    12721 tttgccagtc atgaccagtt aaaagtaagc aatgcacttt atcaagaaat gctagcgctt
    12781 tacgtgaagg gggagtaaaa atgattaaaa cagaccatca cctcctaaag cgagtgcttc
    12841 aggatttgtt aaaaaaacct ctacctgttt gtattctcgt gatagctagt tttgtacaag
    12901 ttggtttaag tgtttacttg cctgttctta tcggtaaagc cgttgatatg agtttgtcag
    12961 tcaattcttg gcaaacttta aaatggctgt tggggcaaat gttagttgtt attgtggtca
    13021 acacattgat tcaatgggtg atgcctcttg tctatagtcg tttgctatat caatatagcc
    13081 agcaattgaa agataagctt ttggaaaaaa ttcatcgtct tccttttgca tatctagatc
    13141 gtcaaaccat tggagatttg gttagtcgag tgataaccga cactgagcaa ctgatcaatg
    13201 gtctacaatt ggtttttaac cagtttatac ttggactatt gacgattctg tgtaccatta
    13261 tagctatggc acagattgac tggctcatgt taatcttggt tttggtgtta acaccaagct
    13321 ccctcttttt agctcgtttt attgctcaaa agagttttca ttatgcacaa gcacagacca
    13381 aaagcagagg aaatttagct cagtttactg aagaaatcct tcgtcaagaa ggtttggttc
    13441 aactgtttaa tgctcaagag cagtctatct gtgattatca tgttttaaat aaaacctatt
    13501 gcgaggcttc acaaaaagcc attttttacg cctcaacagt caatccagcc actcgcttta
    13561 ttaatagtgt catttatgct ttactggcag gtttaggggc tgttcgtatc atggctggac
    13621 ttttttcagt aggtcagcta accacttttt taaatgttgt tgtccaatat accaaacctt
    13681 tcaacgatat ttcatctgtc ttggcagaga tacagagttc tttagcttgt gcgcaaaggc
    13741 tttatgacct tttagatatt gaaataaaag agcaagagca ctttctaaca tttaaggcat
    13801 cagcggttaa gggacaaatt gactttgaag aagtaagttt ttcttatcaa aaagacaggc
    13861 ccttactgaa ggatattaat ttttctgttc ctgcgggttc caaggtagcg attgtagggc
    13921 ctacaggtgc aggcaaatcg actttaatca atttactcat gcgcttttat gaacttgatg
    13981 ctggtagcat caagttagat aaagttccta ttaagtgtta tgctaaggaa gaacttaggt
    14041 ccattactgg catggtattg caagaaacct ggttgaaaga tgcgactgtt catgagttga
    14101 ttgcttacgg cagtgaagag gctagccgtg gtgaagtagt ggcagcagcc aaagcagctc
    14161 atgcacactt ctttattatg caacttccta agacttatga tacttactta agtgcttctg
    14221 atgatgcttt gtcccaaggg cagctccagt tattagctat tgccagaatg tttttgaaaa
    14281 aaccaaaagt cttgattcta gatgaagcca cctcctctat tgatattaga acagaagctg
    14341 ttattcaaga ggcactaaaa gaactcatga gaggaaggac cagctttatc attgcccatc
    14401 gtttatcaac gattcaatca gctgatttga ttcttgttat ggatcaaggt cgattggttg
    14461 agtggggaac acatgccagc ttaatgtcaa aaaacggatg ttatgctaga ttacaaaaga
    14521 tagaataaag ttcgtctttt aacaaaggtg aactttttaa ctcttcctaa agttagttaa
    14581 ttaacaaact ttaaactgtt atataaaaaa ataacagcga aaaagcggac gatctttttt
    14641 tataagtgag aaaaaacagt tgactaaaga agaaataact caaaaaagtg agcaatatat
    14701 ttggaagcgt tttttgactg tggtataata gagccctata taatattgtg aaatgaggaa
    14761 caaaatatat gagtaaaatc gttgttgttg gtgcaaacca tgctggtacg gcctgtatca
    14821 agactatgtt aacaaactac ggtgatgcta atgagattgt tgtatttgac caaaactcaa
    14881 atatttcatt tttaggctgt ggtatggcac tttggattgg tgagcaaatt gctggaccag
    14941 aaggactttt ctattcagat aaagaagaat tagagtcttt aggggctaaa gtttacatgg
    15001 aatcacctgt tcaatcaatc gactacgatg ccaaaacagt gacagcgctt gttgatggta
    15061 aaaaccacgt ggaaacttac gacaagttga tttttgcaac tggctcacaa cctatcttac
    15121 caccgattaa aggtgccgaa atcaaagaag gatcacttga atttgaagca actcttgaaa
    15181 atcttcaatt tgtgaagtta taccaaaact cagctgatgt gattgcaaag cttgaaaaca
    15241 aagacattaa acgtgtagcc gtagttggtg ctggttacat tggtgttgag ttagctgaag
    15301 ctttccaacg caaaggcaaa gaagtggttc tgattgatgt cgtggacact tgcttggcag
    15361 gttattacga ccgtgatttg actgacttaa tggctaaaaa catggaagaa catggtattc
    15421 aactagcctt tggtgaaaca gttaaagaag tagctggtaa tggtaaggtt gaaaagatca
    15481 ttactgacaa aaatgagtac gatgtggata tggttatcct cgccgttggt ttccgtccaa
    15541 acacaactct tggaaatggt aagattgatc ttttccgtaa tggtgctttc cttgtcaata
    15601 aacgccaaga aacttctatt ccaggtgttt atgctatcgg tgactgtgca actatctacg
    15661 ataatgctac tcgcgataca aactacattg ctttagcttc aaatgctgtc cgtacaggaa
    15721 ttgtagcagc acataatgct tgtggtacag accttgaagg aatcggtgtc caaggctcaa
    15781 acggtatttc catttacggc ttgcacatgg tatcaactgg tttgactctt gaaaaagcta
    15841 aacgacttgg ttttgatgct gcagtgactg agtatactga taaccaaaaa cctgaattta
    15901 tcgaacacgg taacttccca gtaaccatta agattgttta cgataaagac tcacgtcgta
    15961 tcttgggtgc tcaaatggca gcccgtgaag atgtgtcaat gggaattcac atgttctcac
    16021 ttgctatcca agaaggcgta accattgaaa agttggcatt aactgatatt ttcttcttac
    16081 cacatttcaa caaaccttac aactacatca caatggcagc acttggtgcc aaagactagt
    16141 atctgatagg aaaaagccag agcagttcag cagcactggc ttttttgctt tttaattgtt
    16201 gcaacctgaa aagttctaaa cttatgatta tcctcttata gcacgttgga aaaggaatat
    16261 caggcaaaac aaaaagagac caaagtctct tttgtgatta gtttttagca gcagaagcaa
    16321 attcttcctt agcaaaggct tcatcaatga tagctttcaa ttgatttgca gaggcttgca
    16381 ttttttgcaa ttcagcatcg ttaagtggaa tgtttactgg acgaacaatt ccatacgctc
    16441 caacgatagc tggttgacca atatagcaat cttcaacacc ttcgtattgg ccttcttgga
    16501 aaacagaaag cggaagaaca gcattttcat catcaagaat agcttttgta atacgagcta
    16561 gggcaactgc aataccgtag aaagtagcac cttttttatt gatgattgag taagcagcat
    16621 cacgtacaga aatgaaaaga tcaactaggc cttgctcatc aatgtcacga ttagcttgga
    16681 gccagtcgta aagacctaca ccagcaacgt tagcgtgtga ccaaacagca aattcagagt
    16741 caccgtgttc tcccatgatg taagcgtgaa ccgaacgcgc atcaacaccg attttagcag
    16801 ccagagcttg acggaaacgg gctgaatcta gagatgtacc tgaaccaatg acacgctctt
    16861 tagggaaacc tgagaatttc catgttgagt aagtcaaaac gtcaactggg ttagcagcaa
    16921 caagaaagat tcctttgaaa ccagatgcaa cgatttgtgt aacaacctct ttattgatac
    16981 gaaggttttt ttcaacaagg tcaaggcgtg tttcacctgg tttttgagga gcaccagctg
    17041 ttaagacaac aagatcagca tcatgacagt ctgagtagtc agctgcatag atttttttag
    17101 gtgatgtaaa agctagagcg tggcttaaat cttctgcatc tccttgagtt ttttctttaa
    17161 aaatatcaat aataccaagt tcttgagcaa tattttgtgt taccaatgca aaagcgtatg
    17221 atgaaccgac ggcaccatca ccgaccaaga ttactttttt gtgttgttta gttgcagtca
    17281 tttctaaaca tctccttaat tttattagag caaaagctca tacggtctta ttttaacata
    17341 ttgataattg tttgtcattg aattcagaaa aataatgaaa atagtttcct gataacgctt
    17401 ccaataagaa aaaataagga atttatggta taatgaaatc tagtattgac tagaaaagga
    17461 gcattcctta aatgcaagat cgaaatttaa ttgacgtcaa tctgacaagt gaaatgaaaa
    17521 cgagtttcat tgactatgcc atgagtgtca ttgtggcaag agctttgcca gatgtgcgtg
    17581 atggtctgaa acctgtgcat cgtcgtattc tctatgggat gaatgaactc ggtgtgacac
    17641 ctgataaacc gcataaaaaa tctgctcgta ttacgggtga tgtcatgggt aaatatcacc
    17701 cgcatgggga ttcttctatt tatgaagcta tggttcgtat ggctcagtgg tggagttatc
    17761 gccatatgct tgttgatggg catggcaatt ttggttctat ggatggtgat ggtgctgccg
    17821 ctcaacgtta tactgaagcg cgcatgagta aaattgcgct tgaattatta agggatatca
    17881 ataaaaatac cgttaatttt caagataact acgatggaag cgaaagagaa ccagtagtat
    17941 taccagctcg ctttcctaat ttattagtca atggagcgac agggattgcc gttgggatgg
    18001 caactaacat tcctcctcat aatttggcag agtctattga tgctgtcaag atggtaatgg
    18061 aacatccaga ctgtacaaca agagagttga tggaagtgat ccctggacct gactttccga
    18121 ctggtgccct tgttatgggg cgctcaggta ttcatagggc ttatgatact ggtaaaggtt
    18181 ctatcgtttt acgctctcga acagaaattg aaacaactca aacaggtcgg gaacgtattg
    18241 tggttactga atttccctat ggcgtcaata aaacgaaagt gcatgaacac attgttcgtt
    18301 tagcacaaga aaaaagacta gaaggtatta cagcagttcg tgacgaatct agccgtgaag
    18361 gagtgcgttt tgttatcgag attcgtcgag aggcatccgc gacagttatc ttaaataacc
    18421 tctttaaatt aacaagcctt cagacgaatt tcagttttaa tatgttggct attgaaaatg
    18481 gtgtgcctaa aatattgtca ctcagacaaa tcattgataa ttacattagt catcaaaaag
    18541 aagttattat acgtcgcacc caatttgata aagataaagc tgaagccaga gctcatattt
    18601 tagaaggctt actgattgcc cttgatcatt tagacgaagt gattgctatt attcgtaaca
    18661 gcgagacaga tgtcattgct cagacagaat tgatgtcacg tttcgacttg tctgaacgcc
    18721 aaagccaagc tattttggat atgcgtttgc gtcgtttgac tggtttggaa cgtgataaga
    18781 ttcaatcaga atatgatgat ttgttggcct tgattgctga cttatctgat actttagcca
    18841 aaccagaacg tattatcact atcatcaaag aagaaatgga tgagatcaag cgtaaatacg
    18901 ctaacccacg ccgtacagaa ttaatggtcg gtgaagtcct ttctctagaa gatgaagacc
    18961 taattgaaga agaagatgtt ttgattacct tgtcaaataa aggttatatc aagcgtcttg
    19021 ctcaagatga atttcgtgcc cagaagcgtg gtggacgtgg cgtccaagga actggggtca
    19081 acaatgacga ctttgtacgt gaattggtat caacaagtac ccatgatact ttgctcttct
    19141 ttacgaattt tgggcgtgtc tatcggttga aagcttacga aattccagaa tatggtcgta
    19201 cagcaaaagg tcttccaatt gttaatctat tgaaattaga agatggtgaa accattcaaa
    19261 ctattatcaa cgcacgtaag gaagaaacag caggtaaatc cttcttcttt acaacgaaac
    19321 aaggcattgt caaacgtaca gaagtttcag aatttaataa tatccgtcaa aacggtctcc
    19381 gtgctcttaa gttgaaagag ggagatcagc tgattaacgt ccttttgaca agcggtcagg
    19441 atgacattat cattggaact cattccggat attctgttcg ttttaatgaa gcaagcatcc
    19501 gcaacatggg ccgatcagct acgggtgttc gcggggttaa gcttcgtgaa gatgatcgcg
    19561 ttgttggcgc gtcacgtatt caagataacc aagaagttct tgtaattacc gaaaatggct
    19621 ttggtaagag aacaagtgct acagactatc ctacaaaagg taggggggga aaaggaataa
    19681 aaacagctaa tattacccct aaaaatggtc aattagctgg attggttacc gtagatggta
    19741 cagaagatat catggtgatt acaaataaag gtgtcattat tcgcacaaat gttgcaaata
    19801 tttcgcaaac aggtcgtgct actttgggtg ttaaaattat gaaactagat gctgatgcta
    19861 agattgtaac atttaccttg gttcagcctg aagacagtag tatcgatgaa atcaatactg
    19921 atcgtgaaaa tagtatttca aaaaataaag ataattaatc ataacaagga gtaaaaaatg
    19981 gtaaaaaaac aaaagcgtcg aaaaataaaa tccatgtctt gggcaagaaa actactaata
    20041 gttgtacttt taattctagg tttagcattg ctttttaaca aacctatccg aaatacatta
    20101 attgctcgaa actctaataa atatcaagta acgaaagttt cgaaaaaaca aatcaaaaag
    20161 aataaagagg ctaagtcaac atttgatttc caagccgtag agccagttag tacagagagt
    20221 gtcttgcaag cacaaatggc ggctcagcaa cttcctgtta tagggggcat tgccatacca
    20281 gagcttggca ttaatttacc aatttttaaa ggtttaggaa atactgagct tatttatggc
    20341 gcaggaacga tgaaagaaga acaagttatg ggaggagaaa ataattattc tcttgccagt
    20401 catcatattt ttggaattac aggttcatct caaatgctct tttcgccgct tgaaaaagca
    20461 caaaatggga tgtccatcta tttaacagat aaagaaaaaa tttacgaata catcataaaa
    20521 gatgttttca cggtagctcc tgaacgcgtt gatgttatcg atgatacagc tggtctcaaa
    20581 gaagtgactt tagtgacttg tacagatatc gaagcaacag aacgtattat tgtcaaagga
    20641 gaactaaaaa cagaatacga ttttgataaa gcgcccgccg atgtattgaa agcttttaat
    20701 cattcttata accaagtatc tacctagata tatcttaaat ataatattta aaaagtagga
    20761 ccatagagaa aatttatctc atcctacttt ttttgaaagg agaaatatga tgaaactaaa
    20821 tgccattcat cacgttgcta ttattgtatc tgattatcat ctgtcaaaag atttttatgt
    20881 gaataaactc ggatttgaaa ttatcagaga aaattatcgt cctgacaagc atgattacaa
    20941 attagattta tcttgtggtc gtattgaatt ggagattttt ggtaaggtga caagtgaccc
    21001 ctactatcaa gctcctccta aacgtgtaag tcaaccagaa tttaaaagtg aggcctgtgg
    21061 tttgagacac cttgcttttc gtgttaccaa cattgagagt tatgtagatg atctaaagag
    21121 cttaggaatt ccagtggagc ctattaggca tgatgattac acaggtgaga aaatgacttt
    21181 cttttttgat ccggatggtt tgcctttaga attacatgaa tagacttatt aggttaacga
    21241 ctggatcgtg ctattaaact cgttaaaaat agttatttgt tgtatcttct tttgcttgat
    21301 taatctcttt tgatcgttaa atgatttggt taactttcaa agtgaagagc aatttttgga
    21361 gtaatcttgt cgaagttttt taatagaata actataggaa aagcaaaata tttgacgata
    21421 aactcttaca
//
LOCUS       DAUDYX010000035        21175 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.035, whole genome shotgun sequence.
ACCESSION   DAUDYX010000035 DAUDYX010000000
VERSION     DAUDYX010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21175
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.035"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(525..911)
                     /gene="rplQ"
                     /locus_tag="VHD02_001274"
     CDS             complement(525..911)
                     /gene="rplQ"
                     /locus_tag="VHD02_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEQ5497881.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(926..1864)
                     /locus_tag="VHD02_001275"
     CDS             complement(926..1864)
                     /locus_tag="VHD02_001275"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEQ5497882.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELEVEGP
                     AEVTAGDILTDSDIELVNPDHYLFTIAEGHSLRATMTVAKKRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKSEPEMMKVRNLGRKSLEEVKVKLADLGLGLKNDK"
     gene            complement(1910..2293)
                     /gene="rpsK"
                     /locus_tag="VHD02_001276"
     CDS             complement(1910..2293)
                     /gene="rpsK"
                     /locus_tag="VHD02_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEQ5497883.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(2311..2676)
                     /gene="rpsM"
                     /locus_tag="VHD02_001277"
     CDS             complement(2311..2676)
                     /gene="rpsM"
                     /locus_tag="VHD02_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEQ5497884.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDIR
                     VKDLTSDQEDAIRREVDAIKVEGDLRREVNMNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(2694..2810)
                     /gene="rpmJ"
                     /locus_tag="VHD02_001278"
     CDS             complement(2694..2810)
                     /gene="rpmJ"
                     /locus_tag="VHD02_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEQ5497885.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(2836..3054)
                     /gene="infA"
                     /locus_tag="VHD02_001279"
     CDS             complement(2836..3054)
                     /gene="infA"
                     /locus_tag="VHD02_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEQ5497886.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(3172..3810)
                     /locus_tag="VHD02_001280"
     CDS             complement(3172..3810)
                     /locus_tag="VHD02_001280"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEQ5497887.1"
                     /translation="MNLLIIGLPGAGKGTQAAKIVEEFGVAHISTGDMFRAAMANQTE
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPSCLVERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNMAQGEPILEHYRKLGLVTDIEGNQEITDVFADVEKALLELK"
     gene            complement(3960..5264)
                     /gene="secY"
                     /locus_tag="VHD02_001281"
     CDS             complement(3960..5264)
                     /gene="secY"
                     /locus_tag="VHD02_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEQ5497888.1"
                     /translation="MFLKILKDALKIKTVRNKIFFTIFIILVFRIGTHITVPGVNAKS
                     LEQLSELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLVLAFAQSIGITAGFNTLSNVALVKTPDIKTYLLIGALLTTG
                     SVIVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAIATIREDYFVNVKASDLHSSYLI
                     VGILIIAVLAIVFFTTYVQQAEYKIPIQYTKLMQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFVQNGRDLPWLNRLQEIFNYQTPVGMIVYALLIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSVRPGRETEQFMSALLKKLATVGAIFLAFISLAPIAAQQALNL
                     SSSIALGGTSLLILISTGIEGMKQLEGYLLKRKYVGFMNTAE"
     gene            complement(5281..5721)
                     /gene="rplO"
                     /locus_tag="VHD02_001282"
     CDS             complement(5281..5721)
                     /gene="rplO"
                     /locus_tag="VHD02_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEQ5497889.1"
                     /translation="MKLHELKAAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRIPKRGFTNINTKEYALVNLDQLNVFDDGTEVTPAILKDAGIV
                     RAEKSGVKVLGNGELTKKLTVKAAKFSKSAEAAIIAKGGSIEVI"
     gene            complement(5936..6118)
                     /gene="rpmD"
                     /locus_tag="VHD02_001283"
     CDS             complement(5936..6118)
                     /gene="rpmD"
                     /locus_tag="VHD02_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002959367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEQ5497890.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVTAISHLVTVEDVK"
     gene            complement(6133..6627)
                     /gene="rpsE"
                     /locus_tag="VHD02_001284"
     CDS             complement(6133..6627)
                     /gene="rpsE"
                     /locus_tag="VHD02_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEQ5497891.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDGNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMIEVPMVGTTIPHEVYTNFGGAKVLLKPAVEGS
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(6646..7002)
                     /gene="rplR"
                     /locus_tag="VHD02_001285"
     CDS             complement(6646..7002)
                     /gene="rplR"
                     /locus_tag="VHD02_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEQ5497892.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKDVSKGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(7107..7643)
                     /gene="rplF"
                     /locus_tag="VHD02_001286"
     CDS             complement(7107..7643)
                     /gene="rplF"
                     /locus_tag="VHD02_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEQ5497893.1"
                     /translation="MSRIGNKVITMPAGVELTNNNNVITVKGPKGELTREFNKNIEIK
                     VEGTEITVVRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKDLEMKGVGYRAQLQGTK
                     LVLSVGKSHQDEVEAPEGITFTVANPTSISVEGINKEVVGQTAAYIRSLRSPEPYKGK
                     GIRYVGEYVRLKEGKTGK"
     gene            complement(7846..8244)
                     /gene="rpsH"
                     /locus_tag="VHD02_001287"
     CDS             complement(7846..8244)
                     /gene="rpsH"
                     /locus_tag="VHD02_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEQ5497894.1"
                     /translation="MVMTDPIADFLTRIRNANQVKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGKNGERVITNLKRISKPGLRVYAKRDDMPKVLNGLG
                     IAIISTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(8395..8580)
                     /locus_tag="VHD02_001288"
     CDS             complement(8395..8580)
                     /locus_tag="VHD02_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEQ5497895.1"
                     /translation="MAKKSMIAKNKRPAKHSTQAYTRCEKCGRPHSVYRKFKLCRVCF
                     RELAYKGQIPGVVKASW"
     gene            complement(8596..9138)
                     /gene="rplE"
                     /locus_tag="VHD02_001289"
     CDS             complement(8596..9138)
                     /gene="rplE"
                     /locus_tag="VHD02_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEQ5497896.1"
                     /translation="MANRLKEKYTNEVIPALTEKFNYTSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEISFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(9162..9467)
                     /gene="rplX"
                     /locus_tag="VHD02_001290"
     CDS             complement(9162..9467)
                     /gene="rplX"
                     /locus_tag="VHD02_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEQ5497897.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVIVEGVGMIKKH
                     QKPNTENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYSKKSGEVLD"
     gene            complement(9546..9914)
                     /gene="rplN"
                     /locus_tag="VHD02_001291"
     CDS             complement(9546..9914)
                     /gene="rplN"
                     /locus_tag="VHD02_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEQ5497898.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(9939..10199)
                     /gene="rpsQ"
                     /locus_tag="VHD02_001292"
     CDS             complement(9939..10199)
                     /gene="rpsQ"
                     /locus_tag="VHD02_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEQ5497899.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(10225..10431)
                     /locus_tag="VHD02_001293"
     CDS             complement(10225..10431)
                     /locus_tag="VHD02_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEQ5497900.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(10441..10854)
                     /gene="rplP"
                     /locus_tag="VHD02_001294"
     CDS             complement(10441..10854)
                     /gene="rplP"
                     /locus_tag="VHD02_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEQ5497901.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(10858..11511)
                     /gene="rpsC"
                     /locus_tag="VHD02_001295"
     CDS             complement(10858..11511)
                     /gene="rpsC"
                     /locus_tag="VHD02_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEQ5497902.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(11524..11868)
                     /gene="rplV"
                     /locus_tag="VHD02_001296"
     CDS             complement(11524..11868)
                     /gene="rplV"
                     /locus_tag="VHD02_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEQ5497903.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKKVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(11884..12162)
                     /gene="rpsS"
                     /locus_tag="VHD02_001297"
     CDS             complement(11884..12162)
                     /gene="rpsS"
                     /locus_tag="VHD02_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEQ5497904.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(12302..13135)
                     /gene="rplB"
                     /locus_tag="VHD02_001298"
     CDS             complement(12302..13135)
                     /gene="rplB"
                     /locus_tag="VHD02_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEQ5497905.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVSLKSKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPDADIKVGNALPLANIPVGTVVHNIELKPGKGGELVRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATIGTVGNEQQSLVNIGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(13153..13449)
                     /locus_tag="VHD02_001299"
     CDS             complement(13153..13449)
                     /locus_tag="VHD02_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEQ5497906.1"
                     /translation="MNLYDVIKKPVITEKSMIALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVNVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(13449..14072)
                     /gene="rplD"
                     /locus_tag="VHD02_001300"
     CDS             complement(13449..14072)
                     /gene="rplD"
                     /locus_tag="VHD02_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEQ5497907.1"
                     /translation="MANVKLFDQTGKEVSSVELNDAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVEGLSFAAPKTAEFAKVLSALSIDTKVLVLVEEGN
                     EFAALSARNLPNVTVATAATASVLDIVNADKLLVTKEAISTIEEVLA"
     gene            complement(14096..14722)
                     /gene="rplC"
                     /locus_tag="VHD02_001301"
     CDS             complement(14096..14722)
                     /gene="rplC"
                     /locus_tag="VHD02_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEQ5497908.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVILIKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(14970..15278)
                     /gene="rpsJ"
                     /locus_tag="VHD02_001302"
     CDS             complement(14970..15278)
                     /gene="rpsJ"
                     /locus_tag="VHD02_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEQ5497909.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGAKVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(15477..16766)
                     /locus_tag="VHD02_001303"
     CDS             complement(15477..16766)
                     /locus_tag="VHD02_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEQ5497910.1"
                     /translation="MIYNRRKIFSLALPSMIENILQMLMGMVDNYLVAQIGLVAVSGV
                     SIANNIISIYQSLFIALGAAVSSLIARSIGENNQNKQLNYMAGVLQVTLLLSVGLGLL
                     SVVGHHQVLEWLGAEASVTLVGGQYLSIVGGMIVSLGLLTSLGAIVRAQGYPKIPMQV
                     SLLINVLNAIFSALSIYVWGFGLLGVAWATVLSRLVGVFLLCQFIPIKQVAKRLMRPL
                     DKIIFDLSLPAAGERLMMRAGDVLIIGIVVRFGTTALAGNAIGETLTQFNYMPGLAMA
                     TATIILVARQLGGGKVTEIRYIIREAFILSTLMMLVMGALTYLLGPSLLPLFTQNTDA
                     QRSAMVVLLFSLLGAPATAGTLVYTAVWQGLGKAKLPFYATTIGMWVIRIGLGYVIGV
                     VWQYGLIGVWMATVLDNTSRWFILSKQFKKYQEITFH"
     gene            complement(17154..18170)
                     /gene="adhP"
                     /locus_tag="VHD02_001304"
     CDS             complement(17154..18170)
                     /gene="adhP"
                     /locus_tag="VHD02_001304"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEQ5497911.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPNVGHGKALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLKVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAVVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIVIFGAGGLGNLAVQYAKKVFNAHVVAVDINNDKLELAKEVGADIVVNGKEIE
                     DVPGYIQEKTGGAHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIKVVGSLVGTRKDLEEAFAFGAEGLVVPIVEKVPVDTAPEVFDEMERGLIQGRKVL
                     DFIS"
     gene            complement(18423..21065)
                     /gene="adhE"
                     /locus_tag="VHD02_001305"
                     /gene_synonym="adhC"
     CDS             complement(18423..21065)
                     /gene="adhE"
                     /locus_tag="VHD02_001305"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEQ5497912.1"
                     /translation="MTEGHNTVETTSVSVTIDALVQKGLAALAEMRKLDQEQVDYIVA
                     KASVAALDAHGELAKHAYEETGRGVFEDKATKNLFACEHVVNNMRHQKTVGIIEEDDV
                     TGLTLIAEPVGVICGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWVETPSLEATNALMNHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDDFVAEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKPATWIAEQAGFTVPEGTN
                     ILAAECKEVSENEPLTREKLSPVIAVLKSESREDGVEKARQMVEFNGLGHSAAIHTAD
                     AELAKEFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNAVGDNVSAVN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTELMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVLTVPGFIAADTGMDVLTHATEAYVSQMANDF
                     TDGLALQAIKIVFDNLEKSVKTADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPASTP
                     EEAVESYAKAVYDLGCRVGIQMNFKAQGIDENEWKEHSRELAYLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYAERPGRRK"
BASE COUNT         6106 a         4486 c         3816 g         6767 t
ORIGIN      
        1 aattttgttg gaaacggctt aaagtgacag attgtaaaag taaaaaagat attttcacct
       61 ccaaattaaa agaggatttt aaaatactta ttaataaaat attgaatagg ggataaaact
      121 aacaatatag tttgagcatc tgtttgcgat gcaagttttt ttcggacatt taaacgaggg
      181 gattacaccg tttatttgcc catgagaggt ctttaaacac ctgcataagg ttttcttatt
      241 ctgatgacct tattgtctct gagaaaactt tataaacgca tcagaagagg cttctgtgtt
      301 attgttttac tcagcttaaa agcgggagtg tcacctcata atacaacaaa acgcctgctt
      361 ataaaaagtt tctatagctt agataccttt cataaacaag cgtcacatat taacaatatg
      421 atgagtgttc ccgctaggaa aaatcctagc ggtagaccag agctagacta agaataatct
      481 ctcgattact ccatcatcat aacactcaac aaaattgata aaaattaaac taattcaata
      541 attgccattg gagcagcatc tccacggcgt ggttctgttt taagaatacg agtgtatcca
      601 ccgttacgtt ctgcataacg aggtgcaatt tctgagaaaa gtttttgaag agctgtcgtt
      661 gatgtgtatt tatcggtagc ttcatcataa ttttctgatg cgatttcgtt acgaacgtat
      721 gcagctgctt ggcgacgagc atgaagatca ccacgtttac caagagtaat cattttttca
      781 actgttttac ggatttcttt tgcacgtgct tctgtcgtta caattgattc gttgatcaaa
      841 agatctgtcg tcaaatcacg aagcattgct ttacgttgtg agctagtgcg tcctagttta
      901 cggtaagcca ttttgtcctc ctatattatt tatcgttttt tagtccgaga cctaagtcag
      961 caagtttaac cttaacttct tcaaggctct tacgtccaag attacggact ttcatcattt
     1021 caggctcaga tttttctgtt aagtcgaaga cagtgttaat gcctgcacgt tttaaacagt
     1081 tatatgagcg tacggataaa tcaagctcct cgatggtgcg gtcaagtacc ttttcatcgt
     1141 tcactttttc agtttctttc attacttcgg tagcttttgc aacctctgtt aaatcagtaa
     1201 acaggtttaa gtgttcgatc aaaactcgag cagatagacc taaagcatct tcaggaataa
     1261 ttgttccgtt tgtcatgatt tcaatcgtta atttatcaaa gccatcattg ctacctacac
     1321 gggcaggttc aacttgataa ttaacttttt taactggcgt atagattgaa tctactgcca
     1381 atgtacccac aggtgcatca tcttttttgt ttccttctgc tggcacataa ccacgttttt
     1441 tagcaacagt cattgttgca cgtagagaat gtccttcagc gattgtaaaa agataatgat
     1501 ccgggttaac aagttcgata tcactgtcag taagaatatc accagctgtt acctctgctg
     1561 gtccttctac ttcaagttcg ataatctttt cgtcttcgac gtatgatttc acagcaagtc
     1621 ccttaacatt aaggataatt tgcatgacat cttcacgtac acctgggatt gtatcaaatt
     1681 cgtgtagtac tccatcaatt ttaattgatg ttactgctgc acctggaagt gaagacaaga
     1741 gtacacgacg aagagaatta cctagagttg ttccgtagcc acgttcaagt ggttcgataa
     1801 caaatctacc gtaatcttta ttttcatcaa tttttgttat tattggtttt tcaaactcaa
     1861 tcattttttc acccctcgaa acgaaacttg tgtactattt gttatatgat tatacacgac
     1921 gacgttttgg aggacgagca ccattatgtg gcacaggggt cacatcacga attgcagtta
     1981 cttcaagacc tgcagctgct agagcacgaa tagctgattc acgacctgaa ccaggacctt
     2041 ttacagtcac ttcaacagtt tttagtccgt gttcttgtgc agattttgca gcagcttctg
     2101 cagccatttg agcagcaaat ggagtagatt tacgagaacc tttgaaacca agagcaccag
     2161 ctgatgacca tgcaagagca ttaccatgca catctgtaat cataacaata gtgttattaa
     2221 atgtagcgtg aatgtgtgca acaccagatt cgatgttctt tttcacacga cgtttacgtg
     2281 ttggtttagc caattgtttt acctcctatt ttattttttc ttacctgcaa tcgcaacagc
     2341 tttcccttta cgagtgcgag cattgttttt agtattttgt ccacggacag gaagtccacg
     2401 acgatgacgg attccacggt atgaaccgat ttccatcaaa cgtttaatgt tcatgtttac
     2461 ttcacgtcga aggtcacctt caactttgat tgcatccact tcacgacgga tagcatcttc
     2521 ttgatctgat gttaaatctt taacacggat atcttcagaa atacctgcag ctgctaagat
     2581 ttttttagat gttgcaagac caattccgta aacataagta agtgaaatta ctacgcgttt
     2641 atcatttgga atatcaactc cagcaatacg agccattttt tctcctttct attttatcct
     2701 tgacgttgtt tgtgttttgg atttgtcgga caaattacca taacacgacc attacggcga
     2761 ataactttac agtattcgca aattggttta accgatggtc ttaccttcat gttttaatcc
     2821 ctccaattat ttcgattatt taaagcggta tgtgatgcgt ccacgtgtca agtcataagg
     2881 actcatttct actgtaacac ggtcacctac taaaatacga atgtaatttt tgcggatttt
     2941 tcctgataca gttgctagaa tttgatgtcc attttccaat tcaacagtaa acattgcatt
     3001 tggcatcgtt tctactactt tgccttcaat ttcaatcacg tcttcttttg ccacgcaaaa
     3061 gtacccccta aatttcgatt tgatgtcctc tgagcacaga ggtaacaatt ataagtcaga
     3121 ctaaactagt atatcattac taatctactt atgcaagtag attagtataa tttatttgag
     3181 ttctagcaac gctttttcaa catctgcaaa aacgtctgtt atttcttgat taccttcaat
     3241 atctgtaaca agaccaagct tacgatagtg ttcaagaata ggttctcctt gagccatatt
     3301 gacgtccaaa cgacgtttaa cagtttcagg cttatcatct tcacgttgat agtaatcttc
     3361 ttctttataa tctactggtg ggttgaacac tttatggaac gtttcaccag ttttacgatt
     3421 gataatacga ccactcaaac gctctacaag acatgatgga tccactttaa tattaataac
     3481 accatctaag cggagtccta gttcttcaag tgttgcatct aaggcgtgtg cttgttcaat
     3541 agtacgtgga tacccatcaa gcaaaaaacc tttttctgcg atatcatcct cagctaagcg
     3601 ctcttttaca atcccgtttg tgacttcatc aggaaccaat tcacctttat caatataact
     3661 tttagctaaa cgtcccattt cggtttgatt agccattgcg gcgcggaaca tatcccctgt
     3721 tgagatgtga gcaacaccaa attcttcaac aatcttagct gcttgggtcc ctttaccagc
     3781 tcccggcaaa cctatgatta aaagattcat cttttcctcc tcttttaaaa taaatacata
     3841 gtaggaaatt attttccttg gtttttgttt tcaaatggca acaagaaatc gtttgatgat
     3901 aaccaagtct tattgtcatt taaaaacaaa atagaaggtt gacaatgtca acctgtattc
     3961 tattctgctg tattcataaa tccgacatat tttctcttta gaagatatcc ctcaagctgt
     4021 ttcatacctt cgataccagt tgaaatcaaa atgagcaaac tagttccacc taaagcaata
     4081 ctagaagaaa ggttgagagc ttgttgtgct gctattggcg ctaaagagat aaatgctaag
     4141 aagatagctc ctacggttgc tagttttttt agcaatgcgg acataaattg ttctgtctca
     4201 cgtccagggc gaacacttgg tatatatgag gaattcttct gaagattttc tgctgtcttc
     4261 tcaggattaa cttgtacaaa ggtatagaag aatgagaaca atataatcaa caaggcgtaa
     4321 actatcattc cgactggagt ttgataatta aaaatttctt gcaaacggtt taaccacggt
     4381 aaatctctac cattttgaac aaaaggaata atcgtacttg ggatagttgt aatcgagcta
     4441 gcaaagataa cgggaataac gccggctgga tttactttta atggaagata tgaacttgta
     4501 ggtgcacctt gcattagctt tgtgtattgg attggaattt tatattccgc ttgttggaca
     4561 tatgttgtaa agaaaacaat agcaagaaca gctatgatta aaatcccaac aatgagataa
     4621 gacgagtgta aatcacttgc cttaacattt acaaaatagt cttcacgaat tgttgcaatt
     4681 gcacttggaa tagacgaaat aatacccgca aagataatca ttgatacgcc attaccaaat
     4741 cctttatctg tgatttgttc tccaagccag gtaacaataa cgctacctgt tgtcagtaat
     4801 gccccgatca gtaaatatgt tttgatgtct ggtgtcttaa caagtgcaac gttcgacaaa
     4861 gtattaaacc ctgcagtgat accaatggat tgggcaaaag ccaaaacaag tgaaatatag
     4921 cgcgtcgctt gatttaattt acgacgtcca acctcacctt gtttgcccca ctcaacaaac
     4981 ttaggtaaaa tatccatctg caataactga acaacgatag aagcagtaat atatgggcta
     5041 acccccattg aaaatactga gaaatttctc atcgcattac cactaactaa gtttaacata
     5101 tttaagaaag ggagttcact taattgctct aagctcttgg catttacacc aggtacggta
     5161 atatgtgtcc caattcggaa tacaagaatg ataaatatag taaagaaaat tttattccgt
     5221 acagtcttta tctttaacgc gtcttttagt atttttaaga acataatgag tatcccctca
     5281 ttagatgact tcgattgaac cacctttagc aataatagct gcttcagcag attttgagaa
     5341 cttagctgct tttacagtca attttttagt caattcaccg ttaccaagca ctttaacgcc
     5401 tgatttttca gctcggacaa taccagcatc ttttaaaata gccggagtca cttctgtacc
     5461 atcatcaaaa acattcagtt gatcaagatt cacaagtgca tattctttag tattaatatt
     5521 tgtaaagcca cgttttggaa tacgacggaa caatggtgtt tgtccacctt caaaacctaa
     5581 acgtacaccg ccaccgctac gagctttttg acctttttga ccgcgaccag atgttttacc
     5641 attacctgat gatgtcccac gaccaacacg gttacgtact ttacgtgagc cttcagcagc
     5701 ttttaattca tgaagtttca ttttttctcc tttttttgta aaatgctagc gcctctatga
     5761 gggaaaagga ctccctatag aaactcgcct atacattaat gatacttaag ttatccaaaa
     5821 taacttaata cagatacgca ctaaccgact actgtttgtt gccaaaaaag caaactacaa
     5881 gagtcggttt tgtgctcgtt tttaattatg aaatcagaga tttcacagtt ataacttatt
     5941 taacatcttc aacggtaacc aagtgagaaa tagctgtcac cataccacgg atagcagcat
     6001 tatcttcttt gactactgaa gagtttaatt taccaagtcc aagagcaaca acagttttac
     6061 gttgttctgg cttacgtccg ataggagact tagtcaaagt aattttaatt tgagccatga
     6121 tgatcccctt tcttatgcta agtcagaaac tgagatgcca cgaagtgcgg caacttcttc
     6181 tgcacgttta agttgtttca atccttcaac agttgcacga acaatgttga ttggagtgtt
     6241 tgaaccaaga gattttgaag taatatcagc aacacctgct aattcgatga cggcacgaac
     6301 tgcaccacca gcagcaactc cagaaccttc tacagctggt ttcaataata ccttagctcc
     6361 accgaagtta gtgtaaactt cgtgagggat tgttgtacca accattggta cttcgatcat
     6421 gtttttctta gcagcttcaa ctgctttacg gatagcttct ggaacttctt gagctttacc
     6481 agtaccaaat ccaacacgac catttccatc accaacaact acaagagctg caaagcgaag
     6541 acgacgtcca cctttaacaa cttttgtaac gcggttaatc gcaacaacgc gttcttcaag
     6601 ttcaactgca ttatctttaa atgccattat tttgtgtcct ccctattaga atttcaatcc
     6661 gttttcacga gctgcatcag ccaaggcttt aacacgtccg tgatagagat atccaccgcg
     6721 gtcaaacacc acttcagaaa tacctttagc cactgcacgt tcagcaacaa gtttgccgac
     6781 tacaacggct tgttctgttt ttgttccttt agaaacgtct ttatcaagag ttgatgcact
     6841 tgcgagcgtt acacccgcta cgtcatcaat aacttgagcg tagatgcctg tattagaacg
     6901 gaatacgttc aaacgtgggc gatcagcagt tccagagagt ttaccgcgga cgcgacggtg
     6961 gcgtttttgg cggattttgt ttttatctgg tttcgaaatc acaattttca cctcttaatt
     7021 ttaatcaatt tgtctcattg acaagttgtc gattgaactt atcgagcatg ttaaggtgca
     7081 atacttccca tacccgacta agaacattat ttacctgttt taccttcttt aaggcgtacg
     7141 tattcaccaa cgtaacggat ccctttgcct ttgtaaggct ctggtgaacg caagctacgg
     7201 atgtaagcag ctgtttgacc aacaacttct ttgttgattc cttcaactga gattgaagtt
     7261 gggttagcaa cagtgaaagt aattccttct ggagcttcaa cttcgtcttg gtgagattta
     7321 cctactgaaa ggacaagttt agtaccttga agttgagcac ggtaaccgac acccttcatt
     7381 tcaagatctt ttttgaaacc ttcagaaaca cctacaacca tgttattcaa gttagcacgg
     7441 gttgtcccat ggattgtttt catttctttt gagtcgttag gacgtacaac tgtgatttca
     7501 gtcccttcaa ctttgatttc aatatttttg ttaaactcac gagtgagttc gcctttaggg
     7561 cctttaacag taataacatt gttgttattt gttaattcaa cgcctgcagg catagtaatt
     7621 actttattac caatacgtga catgtttatt ttctcctgtt aaattgtcaa gccgcatagc
     7681 gactagtttt cacgggggtt tgatagaatg atatacttca atttatattt caaaattgaa
     7741 cacgagctgt ggtctgtgtg aaaaagataa atcttccttg agtgtaaaca cgctacgtca
     7801 gatttcctat tttcactttg tccacgacgc tcttagtatc ttaaattacc aaacgtaagc
     7861 gatcacttct ccaccaacat tcttttggcg agcttctttg tcagtcaaaa gaccttctga
     7921 tgttgaaatg attgcaattc caagtccgtt aagaacttta ggcatgtcat cacgttttgc
     7981 gtaaacgcga agacctggtt ttgaaatacg ttttaagtta gtgataacac gctcaccatt
     8041 ttttccgtat tttaagaaca cacgaatgat gccttgttta tcatcttcga taacttcaac
     8101 attttttaca aaaccttcac gtttaagaat ctcagcaatc ccttttttaa tgtttgaagc
     8161 aggtacttct aacacttcgt gttttacttg attagcatta cggatacgtg ttaaaaagtc
     8221 tgcaattggg tcagtcataa ccatttttta tattctcctc ttactagcag tttgatgcta
     8281 tcacttacta gttaatgttg tccttaaagg actttggtat cagcttatgc tcacaccgtt
     8341 ttaattaagc acaagactta atctgaagaa caaattaagt tttgtgtcat atttttacca
     8401 agaagctttg acaacacctg gaatttgacc tttgtaagcc aattcacgga agcaaacacg
     8461 acaaagttta aacttgcggt aaactgaatg tggacgtcca catttttcac agcgagtata
     8521 agcttgtgta gagtgttttg caggacgttt gttcttagca atcatagatt ttttagccaa
     8581 tttatttacc ccctattatt ttgcaaaagg cattccaagg cccttaagca attcacgtga
     8641 ttcttcgtca gtatttgcag tagtgacgat tacaatatca agaccacgta ctttatcaac
     8701 atcatcaaag ctgatttctg ggaagataag ttgttctttc acaccgagag tgtagtttcc
     8761 gcgtccatca aacgattttg ttggaacgcc atggaagtca cgtacacgag gaagtgaaac
     8821 gctaaccaat ttgtctagga attcgtacat acgttcgcca cggagggtaa cttttgcacc
     8881 gatcgctaca ccttcacgaa gacggaagcc agcgattgat ttcttagctt tagtaataag
     8941 tggtttttga cctgagataa gcgctaattc agctgcagct ttctctaggt tttttgcgtt
     9001 ggatacagca tcaccaacac ccatattgag aacaattttc tcaacttttg gcacagccat
     9061 aactgatgtg taattgaatt tctctgtcaa cgcaggaatt acttcgttag tatatttttc
     9121 ttttaaacga tttgccatta tgcttctcct ttccttcgtg attaatcaag cacttcgcct
     9181 gattttttgc tgtaacgtac ttttttaccg tcaacaactt tataaccaac acgtccagct
     9241 acaccatttt tatctaatac ttgaacatta gagacatgga taggtgcttc tttttcgacg
     9301 atagctcctt gaggattttc agtgttaggt ttttggtgtt ttttaatcat tccaacacct
     9361 tcaacaataa ctttgttaac ttttggaagt gctttaagaa ctacagcttc agtgcctttg
     9421 tccttgccag caattacgcg aactttgtcg ccttttttta caaacattct ggtttctcct
     9481 attatttctt acgccccgat gggcacccta ggaaaaccta ggggactacg tttgttaacc
     9541 tgagattaaa gtacttctgg tgcaagtgat acgatcttca tgtagccacc ctcacgcaat
     9601 tcgcgtgcaa ctgggccaaa gatacgagtt ccgcgaggag ttttatcatc acggatgatt
     9661 acagcagcat tgtcgtcaaa tttgatgtat gaaccgtctg gacggcgtgc accagttttt
     9721 gtacgaacga taacagcttt aaccacatca ccttttttaa ctgctccacc aggagtagct
     9781 tgttttacag aagcaacgat tacgtcaccg atgttagcga atttacgtcc tgaaccacca
     9841 agtactttga tagtcaagat ctcacgagca ccgctattat cagcaacttt caagcgagtt
     9901 tcttgttgaa tcatttcaat tttctccttt tagtttgatt agataataac agctttttcc
     9961 actacttcca caagacggaa acgttttgta gctgaaagtg ggcgagtttc catgatacga
    10021 acgatatcgc cttctttagc aacgttgttt tcgtcatgtg ctttatattt tttagaatag
    10081 ttgatacgtt taccatagac tgggtggtta cgtttagttt caactacaac tgtgattgtt
    10141 ttgtccatct tgtcagacac tacgcgtccg taaagagttt tacgttgatt acgttccatt
    10201 tagaatttct cctttcccaa tctattattt catttctgat tgcacagttt taacacgtgc
    10261 aatttgtttc ttaacttcgt caaggcgagc agtcttttca agttgacctg ctgcagcttg
    10321 gaaacgaaga tcaaacaatt cttttttgag ctcgttttct ttcttagcaa gttcttcttg
    10381 agacaatcca cgaagctctt taacaaaatc tttaatttct tgaagtttca tgtcttctcc
    10441 ttattctgct tcacgtttta cgaatttgca cttaactggt aatttgtggc tagcaagacg
    10501 taatgcttcg cgagcaattt cttcagaaac accagcaatt tcaaacatca ctttaccacg
    10561 tttaactggt gctacccaac cttcaggtgc ccctttacca gaacccatac gtacaccgat
    10621 agcttttgca gtgtatgatt tgtgtgggaa gattttaatc caaactttac caccacgttt
    10681 catgtaacga gtcatcgcaa tacgggcagc ttcgatttga cggtttgtaa tccagtggct
    10741 agttgtggct tgaagtccgt attcaccgaa tgaaacttct ttaccacctt tagcctcacc
    10801 gcgcattttt ccacggaatt cgcgacggtg tttaacacgt ttaggtacta acatttgtta
    10861 tttgcctcct ttagtgtttt tacgagctgg aagaacttct ccacggtaaa tccaaacttt
    10921 aacgccaagt ttaccataag ttgtgtcagc ttcttcccaa gcatagtcga tatcagcgcg
    10981 aagcgtgtga agtggaacag ttccttctga ataaccttca gcacgagcga tatcagcacc
    11041 gttcaaacga ccagaaactt gagttttaat ccctttagct cctgcacgca ttgtacgttg
    11101 gattgcttgt ttttgagcac gacggaaagc aacacgttgc tcaagttgac gagcaatgtt
    11161 ttcaccaaca aggtgagcat ctaaatcagg ttgtttgatt tcgatgatgt tgatgtgtac
    11221 ttgttttcca gttaatttgt taagttgacc acgaagagcg tcaacgtttg cgccaccttt
    11281 accgataacc atacctggtt tagcagtgtg aagtgaaaca ataactttgt ttactgcacg
    11341 ttcaatttca atagttgaaa ctgatgcgtc agctaattct ttattaatga atttacggat
    11401 tgcaagatct tcatgaaggt aatccgcgta ttctttttca gcataccatt tcgcatccca
    11461 gtcacggatg attccgacac gcataccaat tggatgtact ttttgaccca cgattttacc
    11521 tccttatttt tctgatacaa ctacagttac gtgagttgta cgtttgttga ttggtgaagc
    11581 tgaacctttc gcacgtggac ggaaacgttt cattgttggt ccttcgttag cgaatgtttc
    11641 agatactacc aagtttgctt tttccaaacc aaagttgttt tctgcgttag caattgctga
    11701 gttaagagtt ttctcaataa cacgagctgc tttgtttgga gtgaatttta agattgcgat
    11761 tgcgtcagca actttcttac cacggataag atcaagtact aaacgtgttt tacgaggtga
    11821 aacacgcact gtacgagcca ttgctttagc tgaagtaatt tctgccattg tgtcctcccc
    11881 ctattaacga cgtgttttct tgtcgtcagc tgcgtgacct ttgtaagtac gcgttggtgc
    11941 aaattcacca agtttgtgac ctaccatgtc ttcttggatg taaacaggta catgtttacg
    12001 tccgtcataa actgcgattg tatatccgat gaaacttggg aaaatcgttg aacgacgtga
    12061 ccaagttttg attacttttt tcttttcgtc gtttgcttga gcttcaactt ttttcatcaa
    12121 atgctcatcg acgaaaggtc cttttttaag actacgtccc attttgtagt tttctccttt
    12181 aaataatgta ccacagcggc ttgagttcaa gagcaagact ccaaaactct accgagttgg
    12241 cggaataaat agcaaagcta ttgtaacgct atttcagcaa gttttaagca acttactaag
    12301 attatttttc gttacgacga cgaacgataa gtttgtctga tttagctttc ttgttacgag
    12361 ttttaagacc aagcgctggt ttaccccatg gagttgatgg cgctttacgt ccaactggtg
    12421 ctttaccttc accaccaccg tgtgggtgat cgttagggtt cattacagaa ccacgaactg
    12481 ttgggcggat acctttccaa cggctacgtc ctgccttacc aatgttaaca agtgattgtt
    12541 gttcgttacc aacagtaccg atagttgcac ggcatgtacc aagaatcata cgaacttcgc
    12601 ctgattgaag acgaacaaga acgtatttac cttcttgacc aagtacttga gcagaagctc
    12661 cagctgcacg aacaagttct ccacctttac caggtttcaa ctcaatattg tgaacaactg
    12721 taccgacagg aatgtttgct aatggaagtg cgttaccaac tttgatatct gcatctggac
    12781 cagaaacaat acgttgacct acttcaagac ctttaggtgc aatgatgtaa gctttcacac
    12841 cgtcagtgta atgtacaagt gcgatgtttg cagtacggtt tggatcatat tcgattgttt
    12901 taacaactgc ttcaacgcca tctttgttac gtttgaagtc gatcaaacgg taatgacgtt
    12961 tgtgtccacc accttggtga cgaactgtga tgcgaccatt gttgttacga ccagctttgc
    13021 tcttaagaga aacaagcaat gatttctcag gcgtgcttgt tgtgatttcc gcgaaatcca
    13081 aagaagtcat gttacgacgg ccatttgtcg ttggtttata aactttaata cccacgttat
    13141 ttcctcctta gattattcag cttcagctgc gaacaactcg attgctttag aatcagctgt
    13201 aagagtgatg atagcttttt tagtttttga agtgaaacct gtgtaacgac caacgcgttt
    13261 tgcttttggt ttaacgttaa cagtgtttac acttgcaact ttaactccgt caaaagcagc
    13321 ttcaacagct tgtttgatca aaagtttgtg tgcacgagta tcaacttcga aagtgtattt
    13381 gcctgcttca agagcaatca ttgacttctc agtgataact ggttttttga ttacgtcgta
    13441 caaattcatt atgcaagaac ctcctcaatt gtagagattg cttctttagt aacaagaagt
    13501 ttgtctgcgt tcacgatatc aagaacactt gcagttgctg cagttgcaac agttacgttt
    13561 ggaaggttac gtgcagaaag tgctgcaaat tcatttcctt cttcaacaag aacaagtact
    13621 tttgtatcaa tgctaagagc tgaaagcact tttgcaaatt cagcagtttt tggtgctgca
    13681 aatgaaaggc cttctacagc tacaaattta tcttcagcaa cttttgctga gtaaactgat
    13741 ttcaacgcaa ggcgacgaac tttttgtgga agtttgtatc cgtatgaacg gggagttggt
    13801 ccaaagacaa caccaccacc acgccattgt ggtgagcgga tagaaccttg acgagcgcgt
    13861 ccagttcctt tttgacgcca tggtttacgt ccaccacctg atactgctga acggttttta
    13921 accgcatgag taccttggcg aaggctagcg cgttggctaa ttacaacatc aaaaacaact
    13981 gattcgtttg gttcgatacc gaagatagcg tcgtttaatt caactgagct aacttcttta
    14041 ccagtttggt caaatagttt tacgtttgcc attttaactg ttttctcctt tcttattatt
    14101 tagcagcttt aactgctgac ttgatagtga taagagattt cttagcacct ggtacgttac
    14161 ctttaataag gataacgttc ttttctggga taacttgtac aatttcaagg ttttgaactg
    14221 ttacacggtt accacccata cgtcctgcca agcgtttgtt tttgaaaacg cggttaggcg
    14281 caacaggtcc catagaacct gggcgacgat ggtaacgaga accgtgagcc ataggaccac
    14341 gtgattgacc atggcgtttg ataacacctt ggaaaccttt accttttgat gtccctgtga
    14401 cgtcaacaac atcaccagct tcaaattgtt ctacagataa ttctgcacca acttctaagc
    14461 cttcaatgtt tttgaattca cgaatgaagc gcttaggagc tgtgtttgct tttgcaacat
    14521 ggcctttggc aggtttgtta ctcaagactt cacgtttgtc atcaaaacca acctgaactg
    14581 cttcataacc gtctgtttca acagttttaa cttgaagcac aacgtttgga gttgcttcaa
    14641 tgacagtaac agggatgaat tcgcctgatt cagtgaagat ttgagtcatt cccacttttt
    14701 tccctaagat tccttttgtc atgagaaaat atttcctttt cttaataata tttcaaaaag
    14761 ttttttatga gcgttttttg tgctcaaaaa agttttttac gagcgttttt tgtgctcata
    14821 gcaagataca aagagcgtca aactcaaaat aaaaatagaa aatctaacac aaaaacttct
    14881 gtttcgagga agatttatct ttttcatgta gagtttagct cgtgttcaag ttccatcaaa
    14941 cactaactgt acttgcaaaa tctcaccgat taaagtttga tttctacgtt gacaccactt
    15001 ggaagatcaa gtttcatcaa agcatcaaca gttttttgtg ttggattgat gatgtctacc
    15061 aaacgtttgt gtgtacgcat ttcaaattgt tcgcgagaat ctttgtattt gtgagtcgca
    15121 cgaataattg tgtaaagact gcgttcagtt ggaagtggaa ctggtccagc aactttagca
    15181 cctgtacgtg ttgcagtttc aacgattttt tctgccgctg tatcaagtgt acggtgttcg
    15241 tacgctttca aacggatacg gatttttttg tttgccatct tttctcctct cgtctattta
    15301 cgataatagg ctagctcctc aagaaaaccg acacctgttg cgtggcaatg caaccgagcg
    15361 tgtcgcaacc tcttgtatca ttgctaaagc tgtattttta cagcaccaca atataataac
    15421 agaatcattt agtcattgca agctttttag ggcatttttt caaaaaaact accttcctaa
    15481 tgaaaggtaa tttcttggta ctttttaaat tgtttagaga gaataaacca ccgactggta
    15541 ttatctaaca ctgtcgccat ccagacccct atgagtccat attgccaaac cacaccaatc
    15601 acataaccaa ggccaatgcg aatcacccac attcctatgg ttgttgcata gaagggaagc
    15661 tttgcttttc ccaaaccctg ccagacggct gtataaacta aagtgcctgc cgttgcggga
    15721 gctcctagta aagaaaaaag taaaacaacc atggcggacc tttgagcatc agtattctga
    15781 gtaaatagcg gcaagagact aggacctagc agataggtca aggctcccat aaccagcatc
    15841 atgagggtgg ataaaataaa agcttctcta ataatatacc ttatctcagt caccttaccg
    15901 ccccccagtt gcctggccac tagaataatt gttgccgttg ccatggctaa gccaggcatg
    15961 taattaaact gagtcaaggt ttctccaata gcattacctg ccaaggccgt tgtcccaaaa
    16021 cgaacaacaa tgccaataat taggacatct cctgctctca tcatcaaacg ttcaccagca
    16081 gcaggtagtg ataaatcaaa aataattttg tccaaaggtc tcatcaatcg tttcgcaacc
    16141 tgtttaatag gaataaactg acaaagtaaa aacacaccca ctaaacgaga taaaaccgtc
    16201 gcccaagcaa caccaagaag accaaatccc catacataga tagacaaggc agaaaaaatg
    16261 gcattgagta cgtttattaa taagctcact tgcataggaa tcttaggata cccttgagct
    16321 cgaacaatag ctcctaagct agttaaaagt cctagactaa caatcatacc gccgacaatg
    16381 gatagatatt gccctccaac caatgttaca gaagcttcag ctcctagcca ttctaacacc
    16441 tgatgatgtc caacaactga taacaaccct aagccgaccg aaaggaggag tgttacttgc
    16501 aagacacctg ccatatagtt taattgtttg ttctgattat tttctccaat acttcgagca
    16561 atcagactag atacagcggc tcccagcgcg ataaagaggg actgataaat actgataatg
    16621 ttgttagcta tggagacacc agagactgct acaagtccaa tttgagccac caggtaatta
    16681 tcaaccattc ccattaacat ctgtaaaata ttttctatca tagaaggcag agctaaagag
    16741 aaaatttttc ttctattata tatcatttat gtcccctaat aaaaaattct tgatcaaacg
    16801 atttttttaa acacagtcac tggactcatt cagttatttt gttccattaa tgaattcata
    16861 aagtctacct taatagcaga gtcccaagat acacgacact tagtgttaca gtaacaattg
    16921 accacatgcc aatccttaac atttacctaa cgtttataac taagaagacg aagaacaaaa
    16981 ggactccaaa cgaatttttt ctattatata atatattctt catttagcaa ctttcaatgt
    17041 atcaaaatag cactctcgag tcaaaaatgt tttctgatac tcgagagtgc tattttattt
    17101 ggaaccaaat ttaagcaact taggcttatc tcatatttaa tacatttgta tgtttagctg
    17161 atgaaatcta agaccttgcg cccttggatt aaaccacgtt ccatttcatc aaagacttct
    17221 ggagcagtgt caactggaac tttttcgaca atcggaacaa ccagaccttc tgcaccaaag
    17281 gcaaaggctt cttccaagtc tttacgtgtc cctacaagtg atccgaccac tttgatacca
    17341 tctaaaacag tttttacgat agacagttcc atgtattctg atggaagacc tacggctaca
    17401 actgtcccac cggcacggac actatcaatt gcttggttaa aagcaacttt agaaacagcc
    17461 gttaccacaa caccatgtgc tccaccggtt ttttcttgaa tatagcctgg aacatcttca
    17521 atttctttgc cgttaactac aatatctgcg ccaacttctt tagctaactc aagtttatca
    17581 ttattaatgt ccactgcgac aacatgtgca ttaaagactt ttttagcgta ttgaacagcc
    17641 agatttccaa ggccacctgc cccaaagata acaatccatt ggcctggagc agcacctgcc
    17701 tctttaatag ctttataagt tgttacacca gcacaagtaa tggaagaagc ttgagccggg
    17761 tctaaacctt ctggaacttt aactgcataa tcagctgtta ctactgcata ttcactcatt
    17821 cctccatcaa cactgtatcc agcattttta acactacgac aaagtgtctc acgtcctgta
    17881 gtacagtatt cgcaatgacc acatccttca aagaaccatg caatagagac acgatcacca
    17941 acctttaagg atgttacgcc ttctccaatt tcttctacta taccaatacc ttcgtgcccc
    18001 aaaatacgtc ctggtacctg accaaaatcg ccatgtgcaa cgtgtaaatc agtatgacaa
    18061 acgccacagt attctacttt aacaagcgct ttaccatgac caacatttgg aagatcatgc
    18121 tcaacaactt cgacacctgt actagcttga ttaacaacta cggctttcat atcagtcctc
    18181 catctaataa tgactttttt tgtaatcggt tacaaataaa ttgtacatct tttcacaatg
    18241 aaagtcaata gaaacctgaa aagtttctga aacttaactg gttaatctta tagctgtttg
    18301 cctatctgca aaatacttaa ctacattttc tgacactgga tataaatgat atatttagaa
    18361 aataaaatga tttcttatga ctttaatcat aagaaatcat ttcctggtat caacattaaa
    18421 aatcacttac gtcgtcctgg acgttcagca taaccatagt aagcatcttc aataatttct
    18481 tgcatgtgat caaccattgg aagacgtggg ttagcaggtg aacattggtc ttcataagca
    18541 aggtaagcca attcacgaga atgttctttc cattcgtttt cgtcgatacc ttgagctttg
    18601 aagttcattt gaataccaac tcggcaaccc agatcataaa cagctttcgc ataagattcc
    18661 actgcttctt ctggtgtaga agctggaagc cctaacaatt tcgcaatgtc ttggtatttt
    18721 tcatccgcac ggtagtaatt atacttaggc catgttgctg ttttagctgg acgagttcca
    18781 ttatagcgga taacatacgg caaaagaatt gcatttgtac gtccgtgtac ggtatggaat
    18841 tgtgcgccaa ttttatgcgc cattgagtga gaaataccta agaatgcatt ggcgaaagcc
    18901 atacctgcca tagttgacgc attgtgcatc ttttcacgtg cttcaaaatc ggctgtttta
    18961 actgattttt caagattatc aaaaacaatt ttaatggctt gaagagctaa accatccgta
    19021 aagtcattgg ccatttgtga cacgtaagct tcggttgcgt gtgtcaaaac gtccatacca
    19081 gtatcagcag caataaagcc tggaacagtc aataccaaag ctgggtcaac aatggctaca
    19141 gttggtgtta atgagtaatc agcgattgga tatttacgat tatttgcttt gtcagagata
    19201 acagcaaatg gtgtcacctc tgaacctgtt ccagaagttg ttgggatagc aacaaatttt
    19261 gttttcttac caagttctgg gaatttgaac gcacgtttgc ggatatccat gaatttttga
    19321 acgaggtcat ggaaatcaac ttctggttgc tcgtagaaga gccacataac tttggctgca
    19381 tccataggag atcccccgcc aagggcaata atggtgtctg gtttgaaggt acgcattaat
    19441 tcagtacctt tcataacagt tgtaatgtct ggatctggtt ctacttcagc aaagatctga
    19501 taaacaactt tattgcgacg aagatcaagt tgttcaatga tacgatccaa gaaaccaagc
    19561 tcaaccatag cgtgatctgt aacaatcatg acacgttcaa cgtcacgaca tttttgcaaa
    19621 tattgaattg aatcacgttc gaagtatgtt tttgaaggga ctttaaacca ttgcatatta
    19681 tttctacgtc ttcctacttt tttgatgttc aagagattaa cagcactaac gttatcacca
    19741 actgcgttac gtccatacga accacaacca agagtcaatg atggtaagaa agcattgtaa
    19801 acatcaccga taccaccaaa tgtagaaggt gaattccaga tgacacggat agcacgaatt
    19861 ctagtaccaa attcttttgc caactctgca tctgctgtat gaatggcagc tgagtgacca
    19921 agtccattaa attcaaccat ttgacgagct ttttcaacac cgtcttcacg tgattcagat
    19981 ttcaaaacag caatgactgg agataatttt tcacgtgtta aaggctcatt ttctgaaact
    20041 tctttacatt ctgcagcaag aatattggtt ccttctggaa cagtaaatcc agcttgttct
    20101 gcaatccaag tagctggttt tcctacaata tttggattta attttgcacc agcacagttt
    20161 ttgctatttg ctttggcacc aaaacagaat tcttcaagaa gggctttttc ttttttattt
    20221 acaaaataag tatggtaaga tttgaattct gctacaaaat cgtcatagat ttctttatcg
    20281 atgataacag cttgttcaga ggcacagacc ataccattat caaatgattt agacatcaca
    20341 atatcatgag cggcttgacg gatattagct gatttttcaa cataagctgg aacgttacca
    20401 gcacctaccc caagggcagg tttcccacat gagtatgccg ctttaaccat cgcatttcca
    20461 cctgtcgcaa ggatagtggc aatgccgtca tgattcatca aagcatttgt tgcttcaagt
    20521 gatggtgttt caacccactg cacacaattt tcaggagcac cagcggcaat agcagcatca
    20581 cgaacaatac gagcggcatg tgctgatgat tcttgtgctg atgggtgaaa ggcaaaaata
    20641 attggattac gtgtttttaa cgagatcaaa gatttaaaga tagctgttga agttgggtta
    20701 gttgtgggtg taatcccaca gataacacca accggctcag caattaatgt caaacctgtc
    20761 acatcatctt cttcaataat tcctactgtt ttttgatggc gcatgttatt aaccacatgc
    20821 tcacaagcaa atagattttt tgttgcttta tcttcaaaga caccgcgacc tgtttcttca
    20881 taagcatgtt tagcaagttc accgtgagca tccaaagcag caacagaagc tttggctacg
    20941 atatagtcaa cttgttcttg gtcaagctta cgcatctctg caagagcagc taaacctttt
    21001 tgaacaagag catcaattgt cacagaaaca gaggttgttt caacagtatt atgtccttca
    21061 gtcatttgag actcctttat cttgttaaaa gtttcacaat tttgtttaca aaattattat
    21121 agcacttttt gaaattttgt aaatcatttc acaataaatt tgtgaagttt ttttc
//
LOCUS       DAUDYX010000036        21082 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.036, whole genome shotgun sequence.
ACCESSION   DAUDYX010000036 DAUDYX010000000
VERSION     DAUDYX010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21082
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.036"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            49..675
                     /locus_tag="VHD02_001306"
     CDS             49..675
                     /locus_tag="VHD02_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ5497913.1"
                     /translation="MKKYLKKIGKMMTKMYYDENPDSLHDIHEVKVELLNHPFTFLTD
                     SGVFSKKMVDFGSQVLLETLNFKENERVLDLGCGYGPLGISLAKVQRVDATLVDINNR
                     ALDLARKNATNNQVAVTIFQSNIYENISGHFEHIISNPPIRAGKRVVHSIIEKSIDFL
                     VVNGDLTIVIQKKQGAPSAKAKMATIFGNVEILRKDKGYYVLRSIKQA"
     gene            1326..1715
                     /locus_tag="VHD02_001307"
     CDS             1326..1715
                     /locus_tag="VHD02_001307"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEQ5497914.1"
                     /translation="MGTTDLVSCAVQASEYAYVPYSHFPVGAALKTKDGTIYTGCNIE
                     NVSFGLTNCGERTAIFKAISDGHKELVEIAIYGETMQPVSPCGACRQVMAEFFAPSSL
                     VTLIAKNGQTVEMTVGDLLPYSFTDLI"
     gene            1809..2861
                     /locus_tag="VHD02_001308"
     CDS             1809..2861
                     /locus_tag="VHD02_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEQ5497915.1"
                     /translation="MNKKFIGLGLASVAVLSLAACGNRGASKGGASGKTDLKVAMVTD
                     TGGVDDKSFNQSAWEGLQSWGKEMGLQKGTGFDYFQSTSESEYATNLDTAVSGGYQLI
                     YGIGFALKDAIAKAAGDNEGVKFVIIDDIIEGKDNVASVTFADHEAAYLAGIAAAKTT
                     KTKTVGFVGGMEGTVITRFEKGFEAGVKSVDDTIQVKVDYAGSFGDAAKGKTIAAAQY
                     AAGADVIYQAAGGTGAGVFNEAKAINEKRSEADKVWVIGVDRDQKDEGKYTSKDGKEA
                     NFVLASSIKEVGKAVQLINKQVADKKFPGGKTTVYGLKDGGVEIATTNVSKEAVKAIK
                     EAKAKIKSGDIKVPEK"
     gene            3000..4532
                     /locus_tag="VHD02_001309"
     CDS             3000..4532
                     /locus_tag="VHD02_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5497916.1"
                     /translation="MTQHVIEMREITKKFGDFVANDHINLNVRKGEIHALLGENGAGK
                     STLMNMLAGLLEPTSGEIVINDKPVQIDSPSKSAKLGIGMVHQHFMLVEAFTVAENII
                     LGNEVVKNGCLDLNQASKDIKVLSEKYGLAINPSAKVSDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPAEIKELMTIMKNLVKEGKSIILITHKLDEIRAVADRVTVIRRGK
                     SIETVDVAGATSQDLAEMMVGRSVSFTTSKKAAEPKDVVLSIKKLEVDENRGVPAVKG
                     LSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVKSGSIMIKNNEVTHLSSRKITELS
                     VGHVPEDRHRDGLILDLSLAENTALQTYYKQPLSQNGILNYTKINDYARQLMKEFDVR
                     GANELVPARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIKERD
                     KGKAVLVVSFELDEILNLSDRIAVIHDGKIQGIVSPENTNKQELGILMAGGSIHKEEG
                     HV"
     gene            4525..5589
                     /locus_tag="VHD02_001310"
     CDS             4525..5589
                     /locus_tag="VHD02_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ5497917.1"
                     /translation="MSKNAQKIAVPLISVLLGFLLGAIIMVIFGYDPIWGYEGLFQIA
                     FGSVKNIGEIFRSMGPLILIALGFTVASRAGFFNVGLSGQALAGWISAGWFALLNPDM
                     PRPLLILMTALIGMIAGGIAGAIPGILRAYLGTSEVIVTIMMNYIILYVGNAIVQRGY
                     PESVKQSIDSTIQVSDNASYQTHWLSALTNNSRINIGIFFAIIAIALIWFLLNKTTLG
                     FEIRSVGLNPHASEYAGMSSKRTIILSMIISGALAGLGGVVEGLGTFENVFVQGSSLA
                     VGFDGMAVSLLAANSPLGIFFSSFLFGVLNIGAPGMNIAGIPPELVKVVTASIIFFVG
                     SHYLIERYIIRPKKLVKGGK"
     gene            5591..6547
                     /locus_tag="VHD02_001311"
     CDS             5591..6547
                     /locus_tag="VHD02_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ5497918.1"
                     /translation="MSLVTIFALLVSSMLIYATPLIFTSIGGTFSERSGVVNVGLEGI
                     MVMGAFSGIVFNLEFAETFGKATPWIAVLVGGIVGLIFSLIHAVATINFRADHIVSGT
                     VLNLLAPSFAVFLVKAMYGKGQTDNIQQSFGKFDFPGLSQIPVIGDIFFKNTSLIGYF
                     AIAFSFFAWFLLYKTRFGLRLRSVGEHPQAADTLGINVYLMKYYGVMISGFLGGIGGA
                     VYAQSISVNFAVTTILGPGFIALAAMIFGKWNPVGAMLSSLFFGLSQSLAVIGAQLPL
                     LEKIPTVYLQIAPYMVTIIILAAFFGQAVAPKADGINYIKSK"
     gene            complement(6760..8478)
                     /locus_tag="VHD02_001312"
     CDS             complement(6760..8478)
                     /locus_tag="VHD02_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEQ5497919.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSAGLIQVIGEDVDIEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVDADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGTTMFNVLTGFKFIAEKIQEFEE
                     KQNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMNKFRENGPKQFNNTDIVLLEDFQKQTATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATIGDTLDIAQEKIANIETE
                     INTFVG"
     gene            complement(8654..9223)
                     /locus_tag="VHD02_001313"
     CDS             complement(8654..9223)
                     /locus_tag="VHD02_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ5497920.1"
                     /translation="MNHRKSADISRIAIFFAIMLVIHFVSSLVFNIWPIPIKPTLVHI
                     PVIIASVLYGPRIGAILGGLMGIISVITNTIILLPTNYLFSPFVDHGTFASLIIAIIP
                     RILIGITPYYCYKLIPNQFGLIVSGIIGSLTNTIFVLSGIFIFFATVFDGNIKALLTA
                     IISSNAIVEMIISAIITFVLIPTLSRLKR"
     gene            complement(9281..9826)
                     /gene="coaC"
                     /locus_tag="VHD02_001314"
     CDS             complement(9281..9826)
                     /gene="coaC"
                     /locus_tag="VHD02_001314"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEQ5497921.1"
                     /translation="MTKHITLAVSGSISAYKAADLTSQLTKIGYDVHIIMTQAATQFI
                     TPLTLQVLSKNAIHLDVMDEHDPKVINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALALPATTPKLIAPAMNTKMYQNPITQENIKRLSTIGFTEIPPKSSLLACGDK
                     GPGALADIDVILATIDTIWKL"
     gene            complement(9819..10511)
                     /gene="coaB"
                     /locus_tag="VHD02_001315"
     CDS             complement(9819..10511)
                     /gene="coaB"
                     /locus_tag="VHD02_001315"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEQ5497922.1"
                     /translation="MTMKLIITSGGTTEPIDAVRGITNHSTGQLGKLITERFLQYHHD
                     VTLVTTKTATKPLPNEHLRIIEVETVNDLMAALKDQVPHHDILIHSMAVSDYTPVYMT
                     DLEQVSQADNLNCFLCEHNSEPKISSASDYQVLFLKKTPKVISYVKQWNPNIKLVGFK
                     LLVNVPQEELIKVARASLAKNHADYILANDLVDIQTGMHKALLISNNEVASADTKEAI
                     ADLLYERMTKHD"
     gene            10692..11543
                     /locus_tag="VHD02_001316"
     CDS             10692..11543
                     /locus_tag="VHD02_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEQ5497923.1"
                     /translation="MKGRIVITIRDLASLPIFVYGDGNKKVPGALSPFVWTEVFLKTI
                     NQEPNQLILHIWPMTRTVILGMLDRQLPYFELAKKEIGNNGYVPVTRNIGGLAVVADD
                     GILNFSLVIPDHFSESISISNAYLIMVDVIRESFSDYYQRIEYHEIKNSYCPGNFDLS
                     IAGRKFAGIAQRRIKKGIVVSIYLSVCGDQAARGQLIKAFYEAGTQGEVTKVNYPQID
                     PECMATLSELLETPFTVAEVLERLRLTLRQLGFSLTEKSPDQALLTNFDAVYERMQLE
                     VVRKEGK"
     gene            11540..12739
                     /locus_tag="VHD02_001317"
     CDS             11540..12739
                     /locus_tag="VHD02_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEQ5497924.1"
                     /translation="MKERFSPLFEPLTLPNGSQLDNRFVLSPMVTNSSTKDGYVTQDD
                     ISYALRRAASAPLQITGAAYVDPYGQLFEYGFSVTKDADISGLKELAQAMKAKGAKAV
                     LQLTHAGRFASYALTKYGFVYGPSYMQLRSPQPHEVKPLTGQQIEELIAAYAQATRRA
                     IQAGFDGVEVSSAQRLLIQTFFSTFSNKRTDSYGCQTLFNRSKLTLAVLQAVQQVIKQ
                     EAPDGFIFGFRATPEETRGNDIGYSIDEFLQLMDWVLNVAKLDYLAIASWGRHVFRNT
                     VRSPGPYYGRRVNQVVRDYLRNKLPVMATGGMNTPDKAIEALAHADFIGVSTPFVVDP
                     EFAHKIKEGCEESIHLRIRPADLKSLAIPQASFKDIVPLMDYGESLPKESRTLFRSLT
                     HNYKEIK"
     gene            12736..13734
                     /locus_tag="VHD02_001318"
     CDS             12736..13734
                     /locus_tag="VHD02_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEQ5497925.1"
                     /translation="MKVSILDYGVIDKEKTPQEALLETRCLAQVADKLGFHRFWVAEH
                     HNIYAFAISSPELLMMHLADHTKQIRIGSGGIMPLHYSSFKIAEWIMTLEALHPNRID
                     LGIGNSLGTTLVQRALSSIHCKDSYSQVVTELYQYLNPDHLSPLPIFVNPRGNTYPQI
                     WTLSNSLETAELAGQLGLGYTFGIFPYIPKDPITEAKRVSAHYRKAFRPSKLLKIPKL
                     ILAVFIVLSDTDEKAEALAKPLDIWMLGQQDFNEFKTYPEVEEARNYHLTEKQREAIA
                     ANRSRMVIGSPHTVKKQLDRLIEACQADELLAIPLVPEFANRQRTLELLADLYVTI"
     gene            13776..14108
                     /locus_tag="VHD02_001319"
     CDS             13776..14108
                     /locus_tag="VHD02_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEQ5497926.1"
                     /translation="MKKIANYLLIEKTDDRYTISMTPELQDDIGTIGYAEFTDNDHLA
                     VDDIILNLEASKTVMSVLSPLAGAVVERNEAATLTPTLLNSEKAEENWIVVLTDVDQA
                     AFDALEDA"
     gene            14101..14913
                     /locus_tag="VHD02_001320"
     CDS             14101..14913
                     /locus_tag="VHD02_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEQ5497927.1"
                     /translation="MPSSFDLLGEMIGLLQTEQLTSSWACPLPNALTKRQDLWRALIN
                     QRPALPLSKDYLNLEDAYLDDWRASFVPVSVKDCQKTNYTSLFLYHGDIRYLAVDAIV
                     NAANSELLGCFIPNHGCIDNAIHTFAGSRLRLACQAIMTEQGRKEAIGQAKLTSAYHL
                     PASYIIHTVGPRITKGHHVSPIRADLLARCYRSSLDLAVKAGLTSLAFCSISTGEFGF
                     PKKEASQIAIKTVLKWQAEHPESKTLTIIFNTFTSEDKTLYDTYLQKENNCE"
     gene            14906..15787
                     /locus_tag="VHD02_001321"
     CDS             14906..15787
                     /locus_tag="VHD02_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEQ5497928.1"
                     /translation="MSNWTTYPQKNLTQAEQLSQLIKEADALVVGIGAGMSAADGFTY
                     IGPRFETAFPDFIAKYQFLDMLQASLFDFEDWQEYWAFQSRFVALNYLDQPVGQSYLD
                     LKEILETKDYHIITTNADNAFWMAGYDPHNIFHIQGEYGLWQCSQHCHQQTYKDDTVI
                     RQMIAEQKNMKVPGQLIPHCPECEAPFEINKRNEEKGMVEDADFHAQKARYEAFLSEH
                     KEGKVLYLEIGVGHTTPQFIKHPFWKRVSENPNALFVTLNHKHYRIPLSIRRQSLELT
                     EHIAQLISATKTIYQKL"
     gene            15834..16853
                     /locus_tag="VHD02_001322"
     CDS             15834..16853
                     /locus_tag="VHD02_001322"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEQ5497929.1"
                     /translation="MYLIEPIRNGKRITDGAVALAMQVYVQENLFLDDDILFPYYCDP
                     KVEIGKFQNAVVETNQEYLKEHHIPVVRRDTGGGAVYVDSGAVNICYLINDNGIFGDF
                     KRTYQPAIEALHHLGATGVEMSGRNDLVIDGKKVSGAAMTIANGRVYGGYSLLLDVDF
                     EAMEKALKPNRKKIESKGIRSVRSRVGNIREYLAPQYQGITIEEFKDLMVCQLLQIET
                     ISQAKRYDLTEKDWQQIDALTERKYHNWEWNYGNAPQYRYHRDGRFTGGTVDIHLDIK
                     KGYIAACRIYGDFFGKADIAELEGHLIGTRMEKEDVLATLNAIDLAPYLGAITAEELG
                     DLIFS"
     gene            16981..18651
                     /locus_tag="VHD02_001323"
     CDS             16981..18651
                     /locus_tag="VHD02_001323"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEQ5497930.1"
                     /translation="MKSDIEIAQSIALQPITDIVKKVGIDGDDIELYGKYKAKLSFEK
                     MKAVEANEPGKLILVTAINPTPAGEGKSTMSIGLADALNQMGKKTMLALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLQQGNDLGIDPRR
                     IIWKRVLDLNDRALRQVIVGLGSPVNGVPREDGFDITVASEIMAILCLATDLKDLKKR
                     LADIVVAYTYDRKPVYVRDLKVEGALTLILKDAIKPNLVQTIYGTPALIHGGPFANIA
                     HGCNSVLATSTALRLADYTVTEAGFGADLGAEKFLNIKVPNLPKAPDAIVIVATLRAL
                     KMHGGVAKSDLAAENCEAVRLGFANLKRHVENMRQFKVPVVVAINEFVADTEAEIATL
                     KALCEEIKVPVELASVWANGAEGGLALAKTVVRVIDQEAADYKRLYSDEDTLEEKVIN
                     IVTQIYGGKAVQFGPKAKTQLKQFAEFGWDKLPVCMAKTQYSFSDNPSLLGAPTDFDI
                     TIREFVPKTGAGFIVGLTGDVMTMPGLPKVPAAMAMDVAENGTALGLF"
     gene            18736..20313
                     /gene="cls"
                     /locus_tag="VHD02_001324"
     CDS             18736..20313
                     /gene="cls"
                     /locus_tag="VHD02_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEQ5497931.1"
                     /translation="MIIKKKAKVKYLLHKGKHGFLRGIFSRTTIIVLLIILQLVFLFQ
                     SYAWMEQYRVWITILESVFAITIVLYLVNSDMDAISRMTWLILIMIAPLLGSLFLIYT
                     KLDWGYRGLKQRINHLVDLSAPYLSDDDAILEVLKDSTSTTYHLVQYLERSRGNFPIY
                     NNTRVTYFPTGETFFDSLKEQLFLAKKYIFLEFFIIAEGQMWGEILSILEKKVSEGVE
                     VRVLFDGMNELSTLSSDYAKRLEQIGIKAKSFLPISPFISTYYNYRDHRKIVVIDGEV
                     SFTGGINLADEYINEVERFGHWKDAGLMLEGEATDSFLILFLQMWSITEKELIIDPYL
                     SDHSLKLPSDGYVIPYGDSPLDTDKIGKNVYIDILNHAKEYVYIMTPYLILDSEMEHA
                     LRFASERGVDIRIIMPGVPDKGVPYALAKTYYKALMTSGVKIYEYQPGFVHSKVFISD
                     NTKAVVGTINLDYRSLYHHFECATYLYRVSVIADIVNDFNEAQKQSLLMTSDHLTQRP
                     WYQKLIGLLVRTIAPLL"
     gene            20653..20991
                     /locus_tag="VHD02_001325"
     CDS             20653..20991
                     /locus_tag="VHD02_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497932.1"
                     /translation="MQIIKRDGHVADFDPDKIYQAIIKAARTVYVIDDTWRQNLTHVT
                     KKVVIDLEEAHVERPTINMVQSLVENRLMDAGYITIAEHYISYRLQRDLERNGYGDKI
                     VVHLRFEQIK"
BASE COUNT         6515 a         3542 c         4366 g         6659 t
ORIGIN      
        1 aattgttaag gaacaagcgt cttgtaaacc atttcaaagt atgatagaat gaaaaagtac
       61 cttaaaaaga taggaaaaat gatgacaaag atgtattatg atgaaaatcc agatagccta
      121 cacgatattc atgaagtaaa agtagagctg cttaatcatc cctttacttt tttgacggat
      181 tcaggtgtct tttcaaagaa aatggttgat ttcggtagtc aagtattatt agagacacta
      241 aatttcaagg agaacgaacg tgtactggac ttaggttgcg gctatggtcc tcttgggatt
      301 agtctagcca aggttcaaag ggtagacgca accttagtgg acatcaataa tcgtgctctt
      361 gatttagcta gaaaaaatgc aactaataac caagtggcag taactatttt tcaatcaaat
      421 atttatgaaa atattagtgg ccattttgag catattatta gtaatcctcc tattagagca
      481 ggaaagagag tagttcatag cattattgaa aaaagcatag attttttagt ggttaatggt
      541 gacttaacga ttgtgattca aaaaaaacaa ggagctccta gcgctaaagc aaaaatggca
      601 actatttttg gtaatgttga gatactaagg aaagataaag gttattatgt attaagaagt
      661 ataaagcaag cctaatttaa ataatcaaca attgctcatt taaattaggt ttgcttaaat
      721 agtaaagagt tatttttaaa ttatagataa ctaatattat actagggtag cccctctact
      781 cgagtaacct tttttaatgt attttaactg aaaaaggctt tcataatttt aaaaaaatgt
      841 taaaataggt gtataacaac tgattaccaa gagattatta tcattatctt ctaaggtgtc
      901 attgttatgg tgacaatcaa ccatacgcta ctaaagatag gtagcgttcg atcacaggtt
      961 gatcaagtga ttgatacaga tatttataat tttacaaata tctgtatcaa tccatgttga
     1021 atagtctatg cttctaaggt gtcgacgtta atgtactgac gggaccgtta tagtctttct
     1081 ttttggagaa acgtcggatg ccagtgaact agttgaagtt aaagatgtta attcaaacag
     1141 tgctgaagag attgatatcg ttttgaacgg tcgtttactt caaagcaaag aaattaccag
     1201 tctatcggag atgatataag tgctaaagtt agatcacgtt aattatctat caagtcgggt
     1261 gcatatttcg ttaaaatatc tacaggggtt tcaatagatg tagctattat gaaaggtgat
     1321 gttaaatggg gactactgat ttagttagtt gtgctgtaca agctagtgag tatgcttatg
     1381 tcccttactc tcattttcca gtaggagcag ctttaaaaac aaaagatgga acgatctata
     1441 ctggctgtaa tattgaaaat gttagttttg gtctgacaaa ttgtggagag cgaacagcta
     1501 tatttaaagc catatctgat ggtcataaag aattggttga aattgctatt tatggagaaa
     1561 ctatgcaacc tgtatctcct tgtggggctt gtcgtcaggt aatggcagaa ttttttgctc
     1621 cttcttcttt agtcacttta attgctaaaa atggtcaaac tgttgaaatg actgttggag
     1681 atttactccc ctattctttt acagatttaa tataatctgc tttaatttct gttatctagc
     1741 cgtcttggct ttatatttac tttcagcaac ttatttgcta gaaatctttt ttaggagggt
     1801 tcatattcat gaacaagaaa tttattggtc ttggtttagc gtcagtggct gtgctgagtt
     1861 tagctgcttg tggtaatcgt ggtgcttcta aaggtggggc atcaggaaaa actgatttaa
     1921 aagttgcaat ggttaccgat actggtggtg tagatgacaa atcattcaac caatcagcat
     1981 gggaaggcct gcaatcttgg ggtaaagaaa tgggccttca aaaaggaaca ggtttcgatt
     2041 attttcaatc tacaagtgaa tctgagtatg caactaatct tgatacagca gtttcaggag
     2101 ggtatcaact gatttatggt atcggctttg cattgaaaga tgctattgct aaagcagctg
     2161 gagataatga aggagttaag tttgttatta tcgatgatat tatcgaagga aaagataatg
     2221 tagccagtgt tacctttgct gaccatgaag ctgcttatct tgcaggaatt gcagctgcaa
     2281 aaacaacaaa aacaaaaaca gttggtttcg tgggcggtat ggaaggaact gtcataactc
     2341 gatttgaaaa aggttttgaa gcaggagtta agtctgttga cgatacaatc caagttaaag
     2401 ttgattatgc tggatcattt ggtgacgctg caaaaggaaa aacaatcgca gcagctcagt
     2461 atgcagcagg tgctgatgtt atttaccagg cagcaggagg cactggagca ggtgtattta
     2521 atgaagcaaa agctattaat gaaaaacgta gtgaagctga taaagtttgg gttattggtg
     2581 ttgaccgtga tcaaaaagac gaaggaaaat acacttctaa agatggcaaa gaagcaaact
     2641 ttgtacttgc atcatcaatc aaagaagttg gtaaagctgt tcagttaatc aacaagcaag
     2701 tagcagataa aaaattccct ggaggaaaaa caactgtcta tggtctaaaa gatggcggtg
     2761 ttgaaatcgc aactacaaat gtttcaaaag aagctgttaa agctattaaa gaagcgaaag
     2821 caaaaattaa atctggtgac attaaagttc ctgaaaaata gtgtttaaga acaagaagcg
     2881 cgacctttgt cggtcgctct ttttaaactg agataatttt tatctcagta aagtttgtga
     2941 taggtgatag atcataggct ttactgaaat aaaagattgc attgaaagga aatgatccta
     3001 tgacacagca tgtcattgaa atgagagaaa tcacgaaaaa atttggtgat tttgttgcaa
     3061 atgaccacat taacttaaat gtcagaaaag gtgagattca tgccttattg ggtgaaaatg
     3121 gggcaggtaa atcaactttg atgaatatgc tagctggtct cttagagcct actagtggag
     3181 aaattgtcat taatgacaag ccagttcaaa ttgattcgcc atctaaatca gctaagcttg
     3241 gtatcggaat ggtacatcaa catttcatgt tagtagaagc cttcacggtt gctgagaata
     3301 ttattttagg aaatgaagta gtaaaaaatg ggtgcttaga tcttaaccaa gcatcgaaag
     3361 atattaaggt actatccgaa aaatatggcc ttgcaatcaa tccatcagct aaagtttctg
     3421 atatttcagt aggagcacaa caacgtgttg agattttgaa aaccttgtat cgtggtgcag
     3481 atattcttat ctttgatgag ccaactgccg ttttaacacc tgcagaaatt aaagaattga
     3541 tgactatcat gaagaatctc gtgaaagaag gaaaatcaat tattttaatt acccataaac
     3601 tggacgagat tcgtgctgtc gccgaccgtg tcacagttat tcgtcgtggt aagagtatcg
     3661 aaacagttga tgtggcagga gcaacttcac aagatttagc tgagatgatg gtgggacgct
     3721 cggtttcctt tacaactagc aaaaaagctg ctgaaccaaa agatgtggtc ttatccatca
     3781 aaaaacttga agtagatgaa aatagagggg ttccagcagt taaggggcta tctctagatg
     3841 tccgagctgg tgaaattgta gggattgctg gaattgacgg aaatggtcaa tctgagttaa
     3901 ttcaagctat tacaggttta cgtaaagtca aatctggaag tattatgatt aaaaacaatg
     3961 aagtcactca tttgtcttca cgcaaaatta ctgagttatc agtgggtcac gtgccagaag
     4021 atcgtcatcg agatggtttg atactagatc tttctttagc tgaaaataca gccttacaaa
     4081 cctattacaa acaacctcta agtcaaaatg gaatcttaaa ttatactaaa attaatgact
     4141 atgctcgtca attaatgaaa gaatttgatg ttcgcggagc caatgagcta gtaccagcta
     4201 gaggattttc tggtggtaac caacaaaagg ccattattgc tcgtgaagtg gaccgtgatc
     4261 cagatttact tattgttagc caaccaacac gtggtcttga cgtaggagct attgaatata
     4321 tccataagcg tttgattaaa gaacgtgata aaggtaaagc tgtcctcgtt gttagttttg
     4381 aacttgatga aatattgaat ttatctgatc gaattgctgt tattcatgat ggtaaaattc
     4441 aaggtatcgt atcgccagaa aataccaata aacaagaatt gggtatttta atggcaggtg
     4501 gttcaattca taaggaggaa ggtcatgtct aaaaatgcgc aaaaaattgc tgtaccattg
     4561 atttctgtct tgctaggatt tttacttggc gctattatta tggttatctt tggttatgat
     4621 cctatctggg ggtatgaagg tttattccaa attgcctttg gtagtgttaa aaatattgga
     4681 gaaatattcc gttcaatggg gccattgatt ttaatagcgc ttggctttac tgttgctagc
     4741 cgtgcaggct tctttaacgt tggtttgtct ggacaggctt tagctggatg gatttcagca
     4801 ggttggtttg cactcttaaa tccagatatg cctcgtccat tattgattct tatgacagct
     4861 cttatcggaa tgattgctgg tggtattgct ggagctattc cagggatact tcgtgcttat
     4921 cttggtacaa gtgaagttat cgttacgatt atgatgaact acatcatttt atatgtcgga
     4981 aatgctattg ttcaacgtgg ttatcctgag agtgtcaaac aaagtattga ttcaactatt
     5041 caagttagtg ataatgctag ttatcaaacg cattggttat cagccctaac taataactct
     5101 cgtattaata ttggtatttt ctttgccatt attgccattg ccctaatctg gttcttgctt
     5161 aacaaaacta ctttaggttt tgagattcgt tcagttggtc tcaacccaca tgcttcagaa
     5221 tacgcaggga tgtcatcaaa acgtactatc attttatcaa tgattatctc tggagctctt
     5281 gcaggtcttg gaggagtagt tgaaggtcta ggaacctttg aaaatgtttt tgtacaagga
     5341 tcttctttag cagttggttt tgacggtatg gcagttagct tattagcagc aaattcaccg
     5401 cttggaatat ttttctcatc attcctattt ggtgtcttaa atattggtgc tccaggaatg
     5461 aatattgcag gcattccacc agaacttgtt aaagtagtaa ctgcatcaat cattttcttt
     5521 gttggttcac attacctcat tgaacgttac attattcgcc ctaaaaaatt agtgaaaggt
     5581 ggtaaataag atgagtttag taacaatttt tgccttattg gtgtcatcta tgcttattta
     5641 tgcaacacca ctcattttca caagtattgg aggaactttc tcagagcgct caggcgttgt
     5701 taatgttggt ttagaaggta tcatggtcat gggagcgttc tctgggattg ttttcaactt
     5761 agagtttgct gaaacatttg gaaaagcaac gccttggata gccgttttag tcggtggtat
     5821 tgttggtttg attttttctt tgattcacgc tgtagcaacc atcaactttc gagctgacca
     5881 cattgtcagt ggtacagtgt tgaacttgtt agcaccttct tttgctgtct tcttggttaa
     5941 agctatgtac ggtaagggac aaacagacaa cattcaacaa tcttttggga agtttgattt
     6001 cccaggttta tcacaaattc ctgtgattgg tgatattttc tttaaaaata ctagccttat
     6061 tggttacttc gccattgctt tttcgttctt tgcttggttt ttgctttata aaaccaggtt
     6121 tggtctgcgt ttacgatcag ttggggaaca ccctcaggca gctgacacac ttggtattaa
     6181 cgtctatttg atgaaatact atggtgtgat gatctcaggt tttcttggtg gaataggtgg
     6241 agctgtttat gctcagtcaa tctccgttaa ctttgctgta acaactatct taggtcctgg
     6301 atttattgct ttggcagcta tgatctttgg taaatggaat ccagttggcg ccatgctatc
     6361 tagtcttttc tttggcttat cgcaaagttt agccgttatt ggtgctcaat taccattact
     6421 agaaaagatt ccaacggttt acttacagat tgcaccttat atggtaacga ttattatttt
     6481 agctgctttc tttggtcaag cagttgcacc aaaagctgat ggaatcaact atattaaatc
     6541 taaataacgt tatattagta agcctcttag tgaggtttaa cagcactcct aaagcaagat
     6601 agttaggtct agctttaagg gtgctttttt gggacttacc gcaagtttgg attgccagtc
     6661 accatcacaa tagaagcaag aatagactaa aaagaactaa gtcagcggct tagttctttt
     6721 tgcttgtctt tcgtttttac tgatagctct taagttgttt tagccaacaa aggtattgat
     6781 ttctgtttca atgttagcaa ttttttcttg agcgatgtca agagtatcac ctattgtggc
     6841 gatatagaac ttgatttttg gttcagttcc tgaagggcga actgcaatcc atgaatcatc
     6901 agctaacgtg tacttcaaaa cattacttgg aggagttgtt aagttagata ttgttccatc
     6961 atttttagta gcggtttgtt tttggaaatc ttctaataat acaatatcag tattgttaaa
     7021 ttgttttgga ccattttcac ggaatttgtt cataatcttc ttaatttcag cagctccatc
     7081 cacacctgaa agtgttactg aaatcgtttt ttcagcaaaa tagccatatt ctttgtaaat
     7141 ttcatcaata ccatctgcaa gtgttaaacc acgcgaacgg taataagccg cgatctcagc
     7201 aaccaaaaga actgcttgaa tagcgtcttt atcacggaca aatggtttaa taagatatcc
     7261 gaagctttct tcaaaaccaa acatataggt gtggttttgt ttttcttcaa actcttgaat
     7321 tttctcagcg atgaatttga atccagttaa aacgttaaac attgtagtgc cataactttc
     7381 ggcaattttt gttactaatt ctgttgatac gattgattta gcgagagcag cattttctgg
     7441 aagtgttcct gctgttttat gagcttccag gatgtatttc gcaataatcg ctccaatttg
     7501 atttccagaa agatttttat aagaaccatc tggttggcga atttcaacgc ctaggcggtc
     7561 tgcatcaggg tcggtagcta ctaagacatc tgcatcaact tctcgtccga gttcttcagc
     7621 aagagcaaag gcagcttggc tttcagggtt aggcgattta acagtagaaa agtctggatc
     7681 tgccttagct tgtgattcga ctacaacaac agattcaaaa ccggcttgag caagggcacg
     7741 gcgtgtcaac atctcaccag ttccgtggag aggagtatag acaattttca tgtcttttcc
     7801 aaagttattg attaaatctt gattgatatt aacatcttta acctctctaa gatattcaat
     7861 atcaacatct tcaccgataa cttgaataag acccgctgat ttgctttcat ctaaatcagc
     7921 aagttcaact gcaaatggat tttcaatagc acggataaag tctgttagtg catcagcatc
     7981 agctggtggt agttgacctc cgtcttgtcc atagacttta taaccattga aaggtgctgg
     8041 attgtggctt gcagtaacca taataccagc ataggcattt aaatggcgaa cagcaaaaga
     8101 gagttctggt gttggacgaa gagcttcaaa tacgtaagat ttaattccat gctgtgccaa
     8161 gacctgtgca gactcaaaag caaattctgg agagaagtgg cgcgaatcat aagcgatggc
     8221 tacaccacgt tttttagctt cctctccctt agtttcgatt aattttgcta atccttcggt
     8281 tgcttgacgg acaacataaa tattaatacg atttgtacca gcaccaatat aaccacgcat
     8341 accagcagta ccaaattcta agtttgtgta aaaagcatct tcttttgttt tttcatccat
     8401 tgatagtaat tcttgacgaa gatagtcagg aagctgctca aaatctaacc atttttgaaa
     8461 attttctgtg taagtcattg aaatactcct tcaatattta taaacgcttt cattatatca
     8521 ttttgaagga aaaaaaacac taattatttg cagctgttta caaaactttt tccatatttt
     8581 caagtagcta atgtaagaaa gttaaaataa ccaagttttt aaaagagaaa aactcggtta
     8641 ttgtgaaagc taattacctt tttagtcttg atagtgttgg aataagtaca aaggtaataa
     8701 tagctgagat gatcatctca acaatagcgt ttgatgagat gatggctgtt agtagtgctt
     8761 taatatttcc atcaaaaact gtcgcgaaaa aaataaatat cccagataat acaaatatag
     8821 tatttgttag agaaccaatg ataccggata ctattaaacc aaactgatta ggaatcaatt
     8881 tatagcaata ataaggggta ataccaatca aaatacgtgg gataatagca attattaaag
     8941 aagcgaaggt tccatgatcg acaaaaggtg aaaagagata atttgtaggc agtagaatga
     9001 tggtatttgt aatgacactt ataatcccca ttaatcctcc taaaatagca cctatccttg
     9061 ggccatataa gacagaagca ataataactg ggatatggac aagcgttggt ttgattggaa
     9121 taggccaaat attaaagaca agactgctca caaaatgaat gactaacatg atggcaaaaa
     9181 agatagcaat acgagaaata tctgctgatt tacggtgatt cattttactc tccaaaaaat
     9241 ataatactta attctaaaaa ggtgatcaga aaaacgactg ttaaagtttc caaatcgtat
     9301 caatagtagc taagataaca tcaatatcag ccaaagcacc aggtccttta tctccacagg
     9361 ctaataagct agattttgga ggaatttctg taaaccctat tgtcgataag cgtttaatat
     9421 tttcttgggt aatagggttt tgatacattt tggtattcat ggctggggct attaattttg
     9481 gtgtggtagc tggtagggca agagcaacac ttgttacaat attatcagcg aatccatatg
     9541 ctagatgagc aatagtatta gcagaggcag gtgcaacaat aaataaatct gtgcgttttg
     9601 ctaattcgat atggttgata acttttgggt catgctcatc cataacatct aaatgaatag
     9661 cattttttga taacacttgc agggttaatg gtgtaataaa ttgtgtagca gcctgagtca
     9721 ttatgatgtg aacatcatag ccaattttag ttaattgact cgtaagatct gctgctttat
     9781 aggcagatat actaccagaa acagctaagg taatatgttt agtcatgttt ggtcatcctt
     9841 tcatataata agtcagcaat tgcctctttg gtatctgcag atgcaacttc gttattggat
     9901 atcaaaagag ccttgtgcat acctgtttgg atatcaacta aatcattggc gagaatataa
     9961 tcagcatggt ttttagctag acttgctctg gctactttaa ttaactcttc ttgaggcaca
    10021 ttaactagaa gtttaaagcc gactagttta atattaggat tccactgttt aacataagag
    10081 ataactttgg gtgttttttt caaaaaaaga acctgataat cagatgcaga tgagattttc
    10141 ggctcactgt tatgttcaca tagaaaacaa ttaaggttat cagcttgaga gacttgttcc
    10201 aaatcagtca tgtagactgg agtatagtca gaaacagcca tactatgaat tagaatgtca
    10261 tggtgaggaa cctgatcttt gagtgcagcc attaagtcgt taactgtttc cacttcaata
    10321 atccttaggt gctcatttgg caggggttta gtggctgttt tagtagtgac tagggtaacg
    10381 tcatgatgat attgtaaaaa tcgctctgtg attaattttc caagttgccc agttgagtga
    10441 ttagtgatac ctcgtactgc atctataggt tcagttgttc cacctgatgt tattattaat
    10501 ttcatagtca tattttatca aaaaaggagc tgatagtaaa cggaagataa tgacgccaga
    10561 cattgtcttg cgtttgtatt aagatggaat agtaatagat ttctagtaat taaaacgaag
    10621 ttctggaaag caagacacta tagtagctaa aatctaaatt ttaaggtaaa atagggcaag
    10681 cggtttcaag tgtgaaagga agaattgtga taactattag agatttagcg tcactgccga
    10741 tttttgttta cggtgatgga aataaaaaag ttccaggagc tttgagtcct tttgtgtgga
    10801 cagaggtgtt tttaaaaact atcaatcaag aacctaatca actgatctta catatttggc
    10861 cgatgacaag aacagttata ttaggaatgt tagatagaca gcttccctac tttgagctgg
    10921 ctaaaaaaga gattggcaat aatggttatg ttcctgttac tcgtaatatt ggcggtttgg
    10981 ctgttgttgc tgatgatggg attttgaatt tttcgcttgt tattccagac catttttcgg
    11041 aatctatcag tattagtaat gcttatttaa taatggttga tgtcattaga gagagttttt
    11101 cggattatta tcagcgtatt gaatatcacg aaattaaaaa ttcttattgc cctggtaatt
    11161 ttgatcttag tattgcaggc cgcaagtttg caggtatcgc ccagcgtcgt atcaaaaagg
    11221 gaattgttgt gtctatttac ttgagcgttt gtggggatca agcagcgcgt ggtcaactca
    11281 tcaaggcttt ttatgaggct ggaacacaag gagaagttac caaggttaat taccctcaaa
    11341 ttgatccaga atgcatggca actctttcag aactccttga gacgcctttt acagtggcag
    11401 aagtccttga acgtcttcga ttgaccttgc gacagcttgg cttttccctc acggaaaaaa
    11461 gtccagatca agccttactg actaattttg atgctgtcta tgaaaggatg cagctagaag
    11521 tcgttagaaa ggaaggcaaa tgaaagaaag atttagtcct ttatttgagc ctttgacttt
    11581 gccaaatggg agtcaattgg ataatcgctt tgttttgtct cctatggtga ccaactcatc
    11641 cacaaaagac ggttatgtca cccaagatga tatatcctat gctttgagac gagcagcttc
    11701 tgcccctcta caaattacag gagcggccta tgtggatcct tatggtcaat tgtttgaata
    11761 tggttttagt gttacaaaag atgccgatat ttctggcttg aaagagctag ctcaagcgat
    11821 gaaggcaaag ggagctaaag ctgtcttaca actgacccac gctggccggt ttgctagcta
    11881 tgccttgact aaatatggct ttgtctatgg gcctagttac atgcagttga gatctcccca
    11941 acctcatgaa gtcaaacctt taacaggtca gcaaatagag gaattgattg cagcttacgc
    12001 tcaggccact cggcgggcaa ttcaagcagg ctttgatggc gtggaagtat cttcggcgca
    12061 gcgcttattg attcaaacgt tcttttcgac tttttcgaac aagaggacag atagttatgg
    12121 ttgtcaaaca ctttttaatc gctctaagtt aactctagct gttttgcaag ctgttcaaca
    12181 ggtgattaaa caagaagctc ctgatgggtt tatttttgga tttcgtgcta caccagaaga
    12241 aactagggga aatgatattg gttatagtat tgacgagttt ttgcagttga tggattgggt
    12301 acttaatgtt gcaaaacttg attatttagc gatagcaagt tggggccgtc atgtatttcg
    12361 aaatactgtt cgttctcctg gcccttatta tggaaggcga gttaatcagg tagttagaga
    12421 ttatttaaga aataagctac ctgttatggc tacaggggga atgaacacac ctgacaaagc
    12481 tattgaagca ctagctcatg ctgattttat tggagtatct acaccttttg tcgtggatcc
    12541 agaatttgct cataaaatca aagaagggtg tgaagagagt attcatttaa ggattcgacc
    12601 tgctgatttg aaaagtttag cgattcctca ggcttctttc aaagatattg tacctttgat
    12661 ggactacggg gaatcattgc ctaaagaatc aagaacttta tttcgtagtc ttactcacaa
    12721 ttataaggag attaaatgaa agtcagtatt cttgattatg gggtgattga taaagaaaaa
    12781 acaccccaag aagctttatt agaaacgcgc tgcttagcac aagtagccga caaacttggt
    12841 tttcatcgtt tttgggtagc agagcatcat aatatttatg cctttgcaat aagcagtcca
    12901 gagctgttga tgatgcattt agcagatcat accaaacaga ttcggatagg ttcaggaggt
    12961 attatgcctc tacattatag tagctttaag attgcagaat ggataatgac tttagaggcc
    13021 ttgcacccta atcggattga tttaggtatt ggcaactctt taggaactac gttagttcaa
    13081 agagctctat caagtatcca ttgcaaagac agctatagtc aggttgtcac agaattatac
    13141 cagtatctta atcctgatca tttgtcacca ctaccgattt ttgttaaccc taggggtaat
    13201 acttaccctc agatttggac gctaagcaat agtttggaga cagcagaact ggctggtcaa
    13261 ttaggtttag gctatacctt tggtattttc ccctatatac caaaagatcc tataacagaa
    13321 gctaaaagag tatcggcaca ttatagaaag gcttttaggc cttcaaagct attaaaaatc
    13381 cccaagttaa tattagcagt ttttattgtc ctttctgata ccgacgagaa agcagaagct
    13441 ttggctaaac cgttggacat ttggatgctt ggtcagcaag attttaatga atttaagacc
    13501 taccctgaag ttgaggaggc tagaaactat cacttaacag agaaacaaag agaagccatt
    13561 gcagcaaatc gtagtcgcat ggttattgga tcacctcaca cagtcaagaa acaattggat
    13621 aggctgatag aagcttgtca agctgacgaa ttattagcca tccccttagt tcctgaattt
    13681 gccaatagac aacgaacctt agagctgcta gcagacttat acgtgaccat ttaaccttac
    13741 caaaaacaat tccctaaact gaaaggaaag aaacaatgaa aaaaattgca aattacttat
    13801 taattgagaa aacagatgac cgctatacca ttagcatgac accagaatta caagacgata
    13861 ttggtactat tggttacgct gaatttacgg ataacgacca ccttgccgtt gatgacatta
    13921 tcttaaatct agaggcttct aagaccgtca tgtctgtctt gtcaccattg gctggggcag
    13981 ttgttgagcg caatgaggca gccacactaa caccaaccct tttgaattca gaaaaagctg
    14041 aggaaaattg gattgttgtg ctaacagatg ttgatcaagc agcttttgat gccttagaag
    14101 atgcctagtt catttgattt attaggggaa atgattggtc tgttgcaaac agagcagctt
    14161 actagttctt gggcctgtcc cttgcctaat gctttaacaa agcgacaaga cttgtggcgg
    14221 gcgctcatta accagcgccc tgctttgcct ttatcaaaag attacttgaa tctggaagat
    14281 gcttatctag atgattggcg tgcttctttt gttcctgtat cagtcaagga ttgccaaaaa
    14341 actaattata ccagtctttt tctttaccat ggggatatta ggtaccttgc tgtggatgct
    14401 attgtcaatg ctgctaatag tgagttattg ggatgtttta ttcctaacca tggctgtatt
    14461 gataatgcga ttcatacgtt tgccggaagt cgtttacgct tagcttgtca ggcaatcatg
    14521 actgaacaag gccgtaagga agccattggc caagcaaagc ttaccagtgc ttatcatcta
    14581 ccagcctctt atattattca taccgtaggg ccaagaatta ccaaaggaca ccacgtttct
    14641 ccgattcgag ctgatttgtt agcacgttgt tatcgttcta gtcttgattt ggcggttaaa
    14701 gcaggattga ctagcctagc tttttgctct atttcaacag gagaatttgg ctttcctaaa
    14761 aaagaagcct ctcagattgc tattaagaca gttttaaaat ggcaagcaga acatccagaa
    14821 tctaagacct taactatcat ttttaatacc tttacatcag aagacaagac cttatacgac
    14881 acttatttgc aaaaggagaa taactgtgag taattggaca acctatcctc agaaaaacct
    14941 cacccaggca gaacaactgt cccaattaat caaagaagca gatgcacttg tagttggtat
    15001 tggagctggc atgtctgcag ctgatggttt tacttatatc gggccgcgtt ttgagacggc
    15061 attcccagat tttattgcta agtatcagtt tttggatatg ctgcaagcta gcctttttga
    15121 ttttgaagat tggcaagaat attgggcttt tcaaagtcgc tttgtcgctt tgaattatct
    15181 tgatcagccc gtaggccagt cttatcttga tttaaaagag attttggaaa ccaaagacta
    15241 tcatattatt actactaatg ctgataatgc tttttggatg gctggttatg atcctcataa
    15301 catttttcac attcaaggag aatatggttt gtggcaatgt agccagcact gccatcagca
    15361 aacttataag gacgatacgg tcatccgtca aatgatcgca gagcaaaaaa acatgaaagt
    15421 cccaggtcaa ctgattcctc attgccctga atgtgaggcg ccttttgaaa tcaataagcg
    15481 taatgaagaa aaaggaatgg ttgaagatgc tgactttcat gcccaaaaag cacgttatga
    15541 ggctttttta tcagaacata aagagggtaa agtgctttat ctagaaattg gtgtgggaca
    15601 tacgacgcct cagtttatca aacacccctt ttggaaacgt gtctcagaaa accctaatgc
    15661 cttattcgtt accttgaatc acaaacatta ccgcatccct ctgtcaataa ggcgacaaag
    15721 cctagaactg acagaacaca ttgcacaatt gattagcgcc acaaaaacca tttaccaaaa
    15781 attgtaacca aagccaatag acggttaatt aagagataga gaaggagact aacatgtacc
    15841 ttattgaacc gattcgtaac ggtaaacgta tcaccgatgg agctgttgct ctagccatgc
    15901 aagtttatgt tcaagaaaat ctatttttag atgatgatat tttatttccc tactattgtg
    15961 atcccaaagt tgaaattgga aaattccaga atgcagttgt agaaactaat caagagtatt
    16021 taaaagagca tcatatccct gttgttcgca gagatactgg cggtggtgcc gtttatgtag
    16081 attctggtgc tgttaatatt tgctacttga ttaatgataa tggtattttt ggtgatttta
    16141 aaagaaccta tcagcccgct attgaagccc ttcatcattt gggagcaaca ggggttgaaa
    16201 tgtctggacg gaatgacttg gttattgatg ggaaaaaggt atctggcgct gcgatgacta
    16261 ttgctaatgg ccgtgtttat gggggatatt cccttttgct agatgtggac tttgaagcca
    16321 tggaaaaagc attaaaacct aatcgcaaaa aaattgaatc aaaaggcatc aggtcagttc
    16381 gtagtcgcgt tggaaacatc agagaatatt tagcaccaca ataccaaggt attacgattg
    16441 aagagttcaa agatttaatg gtttgccaat tgttacagat tgagacgatt agccaagcta
    16501 agcgttatga tttaacggaa aaagactggc aacaaattga tgctttaaca gagcgtaaat
    16561 atcacaattg ggaatggaat tatggcaacg cgcctcagta ccgttaccat agagatggcc
    16621 gttttaccgg tggaacagta gatattcatt tagatattaa aaaaggctat attgcagctt
    16681 gtcgcattta cggagatttt tttggcaagg cagacattgc agaacttgaa ggtcacttga
    16741 taggaacacg aatggaaaaa gaggatgtct tagctaccct caatgcaatt gatttagccc
    16801 cttatttagg tgcgattaca gcagaagaat taggagattt gatattttct tgataaaaaa
    16861 gatacctcag accggtaaag tgcgattaaa aacgaacaat aattgttttg attcaaataa
    16921 atgtacgtat gatatgaatt gtgatataat gacgtcatta cgtaaaattg aggagtcatc
    16981 atgaaatcag atatcgaaat tgcccaaagt atcgccctac aaccaatcac tgacattgtt
    17041 aaaaaagttg gaatagacgg cgatgacatt gagctttatg gcaaatataa ggctaaattg
    17101 tcttttgaga aaatgaaagc tgtcgaagct aatgagcctg gaaaattaat cttggtaact
    17161 gctattaacc ctactccagc aggagaagga aaatcaacca tgtctattgg actggcagat
    17221 gccttaaatc aaatgggaaa aaaaaccatg cttgccttac gtgaaccctc ccttggtcct
    17281 gttatgggga ttaaaggcgg ggcagcaggt ggtggttatg cccaagtatt gcctatggaa
    17341 gacattaatc ttcactttac tggtgacatg catgccatta caaccgctaa taatgcatta
    17401 tctgctttga ttgacaatca tttgcagcaa ggaaacgatt taggaattga tcctcgccgc
    17461 attatttgga agcgcgtgtt ggaccttaat gatcgtgctc ttcgtcaggt aattgttggc
    17521 ttaggcagtc cagtgaatgg tgtgccacgc gaagatgggt ttgatatcac agtagcttct
    17581 gaaatcatgg caattttatg tttggcaact gacttgaaag atctaaagaa acgtcttgct
    17641 gatatcgtcg tggcttatac ctatgatcgt aagcctgtct atgtgcgtga cttgaaggtt
    17701 gaaggagcat tgacattaat tctaaaagat gctatcaagc caaacttggt gcagactatt
    17761 tatggaacac cagctcttat tcatgggggg ccatttgcta atattgctca tggttgtaat
    17821 tctgtccttg ctacctcaac agctttgcga ttagcagatt acactgttac cgaagcagga
    17881 ttcggggccg acctaggagc tgaaaaattc ctaaatatca aagtacctaa tcttcctaaa
    17941 gcaccagatg ctatcgtcat tgttgcaacc ttgcgcgccc ttaaaatgca tggaggtgtc
    18001 gccaaatcag atttagccgc tgaaaattgt gaggctgttc gcttaggatt tgccaactta
    18061 aaacgtcacg tggaaaacat gcgccaattc aaagttccag ttgtggtggc tatcaatgaa
    18121 tttgtagcag ataccgaggc tgaaattgct accttaaaag cgctttgtga agagattaag
    18181 gttccagtag aattagccag tgtttgggca aatggagctg agggaggctt agcattagct
    18241 aaaacagtgg ttcgtgtgat tgatcaagaa gctgctgact acaaacgtct ctattcagat
    18301 gaggatactt tggaagaaaa agtgatcaat atcgttaccc agatttatgg tggcaaagct
    18361 gttcaatttg gccccaaggc caaaacacaa ttgaagcagt tcgcagaatt cggttgggat
    18421 aaattgcctg tttgtatggc taagacccaa tacagttttt cagataaccc atccttgctt
    18481 ggagcgccaa ccgattttga tattaccatt cgtgaatttg ttcctaaaac aggggcaggt
    18541 tttattgttg gtttaacagg tgatgtgatg accatgcctg gtttgcctaa agttccagct
    18601 gccatggcaa tggacgtagc agaaaatggc acagccctcg gtctctttta ggaaaaattg
    18661 ctcgaataat tcgagcaagc gctcctttct tcccgcttta ctgtgatata ataaggtaaa
    18721 tgagattttg gagggatgat tatcaaaaag aaagcaaaag taaaatattt gctccataaa
    18781 gggaaacatg gatttttaag aggaattttt agtagaacaa caatcattgt attattaatt
    18841 attttgcaat tagttttctt atttcaatct tatgcctgga tggagcagta ccgtgtttgg
    18901 ataacgattc ttgaaagtgt ctttgctatt actattgttt tatatttagt taatagtgat
    18961 atggatgcta tttcaagaat gacgtggtta attttgatta tgattgctcc attactaggg
    19021 tcactgtttt taatttatac aaaattggat tggggttaca gagggctaaa acaaagaata
    19081 aatcatcttg tagatctgtc tgcaccttac cttagcgatg atgacgctat attagaggta
    19141 ttaaaggata gcacatcaac cacatatcat ttagtacagt acttagaaag aagtcgtggc
    19201 aactttccga tttataataa tacaagggta acttacttcc caacaggtga aacttttttt
    19261 gatagtttaa aagaacagtt attcttagct aaaaagtaca tttttcttga attttttatt
    19321 attgcagaag gccaaatgtg gggagaaatc cttagtattc tagaaaaaaa agtcagtgaa
    19381 ggtgtagaag ttagggtgtt gtttgatggc atgaacgaac tatcaacgct atcatcagat
    19441 tacgccaaga gattagaaca aatagggatt aaagctaaat catttttacc gatttcaccc
    19501 tttatctcta cctattacaa ttatcgagat caccgaaaaa ttgtcgttat tgatggggag
    19561 gtatcattca ctggaggtat taatctagca gatgagtaca ttaatgaagt agagcgtttt
    19621 ggccactgga aagatgctgg tttaatgctt gagggtgaag caacagacag ctttttaatt
    19681 ttgtttttac aaatgtggtc tatcacagaa aaagaactga ttattgatcc ttatctttca
    19741 gatcattctt taaaacttcc ttcagatggc tatgttattc cctacggtga ttccccgctt
    19801 gatactgata aaataggtaa aaatgtttat atagacattt taaatcatgc taaagagtac
    19861 gtttatatca tgacacctta ccttatttta gatagcgaga tggaacacgc tttacgattt
    19921 gcatcagaac gtggagtaga tatccgaatt attatgccag gagtgccaga taagggggta
    19981 ccatatgcct tagctaaaac ctattataaa gctttgatga cttctggagt aaaaatttac
    20041 gagtatcaac cagggttcgt tcattcaaag gtatttatct ctgataatac aaaagcagtt
    20101 gtcggaacaa ttaacttaga ctatcgtagc ctttatcatc attttgaatg cgcgacttat
    20161 ttatatcgtg tgtcagttat tgctgatatt gtgaatgact ttaatgaagc tcaaaagcaa
    20221 tcactattga tgacatcaga tcatttgacg caacgtcctt ggtatcaaaa attgatagga
    20281 ttattagtac gaacaattgc ccctctcttg taattttcgt aaaatatgtt ataatatctg
    20341 tatgcgatga gtcgattgtg actgcaaagt cacttttgcg caggggaggt cattaaggcg
    20401 ggaagccgac tttgataatc tgtgtgaacc attttatcac aatcttattt attaagaatg
    20461 ccccttggat tgagatattc tcaatccttt ttttaatttt attatcttag ttaaaattta
    20521 gaatgtatca gtgattactg tgtttcaaaa taagaaagta gtttttcggg tatgagtgct
    20581 atgacttgac aaatgctaat aacatttgca taatgtaagt agagatgaaa acttatgaaa
    20641 agagatgatc ttatgcaaat tattaagcgt gatggccacg ttgctgattt tgatccagat
    20701 aaaatttatc aagcaattat taaagcagca aggacggtct atgttattga tgacacgtgg
    20761 cgacaaaact taacacatgt cactaaaaaa gtagttattg atttagaaga agcacatgta
    20821 gaacgtccta ctatcaatat ggtccaatcc cttgtggaga atcgtttgat ggatgctgga
    20881 tatattacca ttgcagaaca ttacatttct tatcgtcttc agcgagattt agagcgcaat
    20941 ggctatggtg ataagattgt ggttcacttg cgttttgaac aaataaaata aaaacgcttc
    21001 taatggcatt ttagtgtctt tgaggggata aggtttaaat acctaacaga taaaaatctg
    21061 ttaggtattt ttttgctact at
//
LOCUS       DAUDYX010000037        20187 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.037, whole genome shotgun sequence.
ACCESSION   DAUDYX010000037 DAUDYX010000000
VERSION     DAUDYX010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20187
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.037"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            138..1106
                     /locus_tag="VHD02_001326"
     CDS             138..1106
                     /locus_tag="VHD02_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEQ5497933.1"
                     /translation="MVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVG
                     EEAANVGAVAHLSYDDIIFSNHRGHGQSIAKDMDLNKMMAELAGKATGVSKGRGGSMH
                     LADFEKGNYGTNGIVGGGYALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHESVNM
                     AATWKLPVIFFIINNRYGISMSINNATNTPHLYTRAEAYGIPGFYCEDGNDVMAVYET
                     MGKAVEHVRGGNGPAIVEVESYRWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLT
                     SEGIATDDELDAIQAQVKKEVDDAYEFAQNSPDPELSVAFEDVWVD"
     gene            1165..2163
                     /locus_tag="VHD02_001327"
     CDS             1165..2163
                     /locus_tag="VHD02_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEQ5497934.1"
                     /translation="MSETKLMALREAVNLAMTEEMRKDENIFLMGEDVGVYGGDFGTS
                     VGMIEEFGPKRVKDTPISEAAISGAAIGAAITGLRPIVDVTFMDFLTIMMDAIVNNGA
                     KNNYMFGGGLITPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGIKVVAPGNANDAKGL
                     LKSAIRDNNIVLFMEPKALYGKKEEVNQDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVLQAAEEVAADGINVEVVDPRTLIPLDKELIIESVKKTGKLMLVNDAYKTGGFIGEI
                     ATMITESEAFDYLDHPIVRLASEDVPVPYARVLEQAILPDVEKIKAAIVKMANKGN"
     gene            2348..3757
                     /locus_tag="VHD02_001328"
     CDS             2348..3757
                     /locus_tag="VHD02_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEQ5497935.1"
                     /translation="MAFEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKIVRPAGDTVPVTEVIGYIGAEGESVDTIASSEKTTEIPVPASAD
                     AGPTVAPKENVASPAPQVAATAIPQGNGGKVRATPAARKAAAEMGIDLGQVPGTGPKG
                     RVHKEDVENFKGAQPKASPLARKIAADKGIDLATVSGTGFNGKVMKEDIKAILEAAKP
                     AEAKAPAAKEEKVVDLPEGVEHKPMSAMRKAISKGMTNSYLTAPTFTLNYDIDMTEMI
                     ALRKKLIDPIMAKTGLKVSFTDLIGMAVVKTLMKPEHEYMNASLINDANDIELHRFVN
                     LGIAVGLDDGLIVPVIHGADKMSLSDFVLASKDVIKKAQTGKLKAAEMSGSTFSITNL
                     GMFGTKTFNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRLVDGMNG
                     AKFMVDLKKLMENPFELLI"
     gene            4084..5847
                     /gene="lpdA"
                     /locus_tag="VHD02_001329"
     CDS             4084..5847
                     /gene="lpdA"
                     /locus_tag="VHD02_001329"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEQ5497936.1"
                     /translation="MAVEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKITRQAGETVPVTEVIGYIGAEGESVEVSSPAASDVNVARTTEDL
                     EAAGLEVPKAPAQAASAAPKAALADDEYDIIVVGGGPAGYYAAIRGAQLGGKIAIVEK
                     SEFGGTCLNVGCIPTKTYLKNAEILDGIKIAAGRGINLASTNYTIDMDKTVDFKNTVV
                     KTLTGGVQGLLKANKVTIFNGLGQVNPDKTVTIGSQTIKGRNVILATGSKVSRINIPG
                     IDSKLVLTSDDILDLREMPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAM
                     DKEVSLELQKILSKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVAEKALLSIGRVP
                     QMNGLENLNLEMDRNRIKVNDYQETSIPGIYAPGDVNGTKMLAHAAYRMGEVAAENAI
                     HGNTTRKANLKYTPAAVYTHPEVAMVGLTEEQAREQYGDVLIGKNSFTGNGRAIASNE
                     AHGFVKVIADAKYHEILGVHIIGPAAAEMINEAATIMESELTVDELLLSIHGHPTFSE
                     VMYEAFADVLGEAIHNPPKRK"
     gene            6156..6233
                     /locus_tag="VHD02_001330"
                     /pseudo
     CDS             6156..6233
                     /locus_tag="VHD02_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6521..8938)
                     /gene="hylA"
                     /locus_tag="VHD02_001331"
     CDS             complement(6521..8938)
                     /gene="hylA"
                     /locus_tag="VHD02_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronidase"
                     /protein_id="HEQ5497937.1"
                     /translation="MNTYFCTHHKQLLLYSNLFLSFAMMGQGTAIYADTLTSNSEPNN
                     TYFQTQTLTTTDSEKKVVQPQQKDYYTELLDQWNSIIAGNDAYDKTNPDMVTFHNKAE
                     KDAQNIIKSYQGPDHENRTYLWEHAKDYSASTNITKTYRNIEKIAKQITNPESCYYQD
                     SKAIAIVKDGMAFMYEHAYNLNRENHQTTGKENKENWWVYEIGTPRAINNTLSLMYPY
                     FTQEEILKYTAPIEKFVPDPTRFRVRAANFSPFEASSGNLIDMGRVKLISGILRKDDL
                     EISDTIKAIEKVFTLVDEGNGFYQDGSLIDHVVTNAQSPLYKKGIAYTGAYGNVLIDG
                     LSQLIPIIQKTKSPIEADKMATIYHWINHSFFPIIVRGEMMDMTRGRSISRFNAQSHV
                     AGIEALRAILRIADMSEEPHRLALKTRIKTLVTQGNAFYNVYDNLKTYHDIKLMKELL
                     SDTSVPVQKLDSYVASFNSMDKLALYNNKHDFAFGLSMFSNRTQNYEAMNNENLHGWF
                     TSDGMFYLYNNDLGHYSENYWATVNPYRLPGTTETEQKPLEGTPENIKTNYQQVGMTS
                     LSDDAFVASKKLNNTSALAAMTFTNWNKSLTLNKGWFILGNKIIFVGSNIKNQSSHKA
                     YTTIEQRKENQKHPYCSYVNNQPVDLNNQLVDFTNTKSIFLESDDPAQNIGYYFFKPT
                     TLSISKALQTGKWQNIKADDKSPEAIKEVSNTFITIMQNHTQDGDRYAYMMLPNMTRQ
                     EFETYISKLDIDLLENNDKLAAVYDHDSQQMHVIHYEKKATMFSNHNLSHQGFYSFPH
                     PVKQNQQ"
     gene            9171..10160
                     /locus_tag="VHD02_001332"
     CDS             9171..10160
                     /locus_tag="VHD02_001332"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007893175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEQ5497938.1"
                     /translation="MKYIVNKSHNPAFNIALEAYAFRELVEEDELFILWINEPAIIIG
                     KHQNTIQEINKEYIDEHGIHVVRRLSGGGAVYHDLNNLNYTIISNKTAEGAFDFKTFS
                     QPVIATLADLGVTANFTGRNDIEIDGKKICGNAQAYYKGRMMHHGCLLFDVDMTVLGD
                     ALKVSKDKIESKGVKSVRARVTNILNELPEKITVEEFSDKILTKMKETYPDMTEYVLS
                     EDELAKIEQSAKEQFGSWDWTYGKAPEYTIERNVRYPAGKISTFANVENSIIKNLKIY
                     GDFFGIKDVQDIENLLIGCKYEYRDVFERLKTIDTTQYFSRMTVEEVAKAIVS"
     gene            complement(10269..11060)
                     /locus_tag="VHD02_001333"
     CDS             complement(10269..11060)
                     /locus_tag="VHD02_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEQ5497939.1"
                     /translation="MTYTSLKSPENTDYLYNLNIAHLYGNLMNTYGDNGNVLMLKYVA
                     EKLGARVTVDIVSLNDAFDQDYYDLVFFGGGQDYEQTIVAKDLPSKKAALADYIAKNK
                     VVLAICGGFQLLGQYYVQANGVKIDGLGIMGHYTLNQHQNRFIGDIKIHNDEFNETYY
                     GFENHQGRTFLYDDEKPLGRVVYGNGNNKEDQTEGVHYKNVYGSYFHGPILSRNVNLA
                     YRLVTTALKEKYGSAITLPSYDDILKQEVAEEYADIKRKAAFDKA"
     gene            complement(11060..12403)
                     /locus_tag="VHD02_001334"
     CDS             complement(11060..12403)
                     /locus_tag="VHD02_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEQ5497940.1"
                     /translation="MKMKTLLGIIAGKAAQSILTKLGRGSTYPGQLALACNKDILKDL
                     SKDYDIVVVTGTNGKTLTTALTVGILKEAFGEIITNPSGANMITGITSTFLAAKKGKS
                     GRQIAVLEIDEASLPRITTYLKPSLFVYTNIFRDQMDRYGEIYTTYQMIVDGARNAPK
                     ATILANGDSPIFSSKDIVNPVQYYGFDTAKHAPQLAHYNTEGILCPKCEHILQYRLNT
                     YANLGDFICLNCGFKRPQLDYRLTELTAITHQSSEFVIDGQNYKINVGGLYNIYNALA
                     AVSVAEFFGVSPEKIKAGFNKSKAVFGRQETFTIGDKSCTLILIKNPVGASQALEMIQ
                     LADYPFSLSVLLNANYADGIDTSWIWDANFELITQMPITEINAGGVRHSEIARRLRVT
                     GFDDTKIKQAEKLEQIMEAIEKQESKHAYILATYTAMLEFRSLLADRHVVEKEMN"
     gene            12510..13361
                     /locus_tag="VHD02_001335"
     CDS             12510..13361
                     /locus_tag="VHD02_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEQ5497941.1"
                     /translation="MNNLSSIDIKFLLSLFADPWLLAVHLLDILIVAYLIYRFIKALT
                     GTKIMSLVQGVIFFLVLRFIAEWIGFTTITYLMNQVITYGVIAGVVIFTPEIRAGLEK
                     FGRSTQVFLQKQYVSSESALVDALIKSVAYMGPRKIGALIAIEQTQTLQEYIATGIPL
                     NADISSQLLINIFIPNTPLHDGAVIVGQNKIVAACAYLPLSESKAISKEFGTRHRAAI
                     GLSENSDALTIIVSEETGAISVTRKGQFLHDLSTDEFETVLRTYLMSDSNVSLPWYKK
                     ILGGKSK"
     gene            13358..14314
                     /locus_tag="VHD02_001336"
     CDS             13358..14314
                     /locus_tag="VHD02_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEQ5497942.1"
                     /translation="MKRFLNSRPWLGMVSVFFAILLFLTAASSNHNNSSSQIYSPIET
                     YTHSLKDVPIDMKYDSDKYFISGYSYGAEVYLTSTNRIKLDSEVNNDTRNFKIVADLT
                     HSHPGTVNVNLRVENLPSGVTATVSPDKISVTIGKKESKVFPVRGSVDAKQIANGYEI
                     SKIETGVNKVEVTSDESTIALIDHVVAKLPDDQVLDRNYSSRVTLQAVSADGTILASA
                     IDPAKTNLSVAVKKITKSVPIRVEAVGMMDDSLSDIQYKLSKQTAVISGSREVLEDID
                     EIIAEVNISDVTKNTSKTVSLSSSQVSIEPSVVTVQLTTTKK"
     gene            14368..15723
                     /locus_tag="VHD02_001337"
     CDS             14368..15723
                     /locus_tag="VHD02_001337"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEQ5497943.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETERPKV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGNDGFKLADDQELEIEALLDAPEDTLPRPSAEGLGTLVDYPEGLRKY
                     EKFLVTTGTDLSGMTVALDTANGAASVSARDVFLDLNAEIAVIGEKPNGLNINDGVGS
                     THPEQLQELVKETGADLGLAFDGDSDRLIAVDETGEIVDGDRIIFIIGKYLSEKGLLA
                     HNTIVTTVMSNLGFHKALDKQGINKAITAVGDRYVVEEMRSSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLAKVMKETGKSLSELAAEVTIYPQKLVNIRVENSMKDRAMEVPAI
                     ANIIAKMEDEMAGNGRILVRPSGTEPLLRVMAEAPTDAEVDYYVDTIADVVRTEIGCD
                     N"
     gene            15847..16488
                     /locus_tag="VHD02_001338"
     CDS             15847..16488
                     /locus_tag="VHD02_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5497944.1"
                     /translation="MTKQQIEKLADLLPPFAATLYLDMIPEFQEGYASYVYQTQKEDI
                     QKRRIKQLTKNFERLLDTPSFYVMPVTRKTAEQLLADKGLAYHLLSTVAANQVFAAAV
                     NEDQTLFQVLRNGARVAFVILQEKGEFLEMTLGILETVTENEVPAAIVEAVQTYLLTD
                     MQEKLPSLSIMVSQESSKLFYQEQGFVLLAKGCKPGNENDSDVCQLVKYFSKP"
     gene            16551..17681
                     /locus_tag="VHD02_001339"
     CDS             16551..17681
                     /locus_tag="VHD02_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEQ5497945.1"
                     /translation="MSKKPTSAYVHIPFCTQICYYCDFSKVFIQNQPVDAYLKALIQE
                     FESYDIHDLKTLYIGGGTPTAITAKQLEYLLNHLERNLNLDDLEEFTIEANPGDLTPE
                     KIAVLQRSAVNRISLGVQTFNNKQLKQIGRSHNEEQIYSTIANLKTAGFHNISIDLIY
                     ALPGQTLEQVKENVAKALALDIPHLSLYSLILEHHTVFMNKMRRGKLNLPTEDLEAEM
                     FEYIISEMEANGFEHYEISNFTKPGFESRHNLMYWDNVEYFGCGAGASGYLNGIRYQN
                     RVPIQHYLKAVEAGNARLNEEVLRKEEMMEEELFLGLRKKTGVSIQRFQEKFGISFEE
                     RYGNIVRELQNQGLLVKDDAFVRMTKKGLFLGDSVAERFILD"
     gene            17691..18443
                     /locus_tag="VHD02_001340"
     CDS             17691..18443
                     /locus_tag="VHD02_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEQ5497946.1"
                     /translation="MGLSYQEELTLPFELCDVKSDIKLPLLLDYCLMVSGRQSAQLGR
                     SNNNLLVDYKLVWIVTDYEITIHRLPHFQETITIETKALSYNKFFCYRQFYIYDQEGG
                     LLVDILAYFALLNPDTRKVATIPEDLVAPFKTDFVKKLHRVPKMPLLEQSIDRDYYVR
                     YFDIDMNGHVNNSKYLDWMYDVLGCAFLKTHQPLKMTLKYVKEVSPGGQITSSYHLDQ
                     LTSYHQIISDGQLNAQAMIEWRAIKQTESEID"
     gene            18443..19207
                     /locus_tag="VHD02_001341"
     CDS             18443..19207
                     /locus_tag="VHD02_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEQ5497947.1"
                     /translation="MPYKGYLIDLDGTIYQGKNRIPAGERFIKRLQEKGIPYLLVTNN
                     TTRTPEMVQSMLAKQFNVETGIETIYTATMATVDYMNDMNRGKTAYVIGETGLKSAIA
                     AAGYIEELENPAYVVVGLDSQVTYEMLAIATLAIQKGALFIGTNPDLNIPTERGLMPG
                     AGALNALLEAATRVKPVFIGKPNAIIMNKSLEVLGIQRSEAVMVGDNYLTDIMAGIQN
                     DIATILVTTGFTRPEEVPTLPIQPDHVLSSLDEWRL"
     gene            19207..19839
                     /locus_tag="VHD02_001342"
     CDS             19207..19839
                     /locus_tag="VHD02_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEQ5497948.1"
                     /translation="MVENTKLLCSWVWLLALAILITIYSTWLWYPLEVDHLKLEQVVF
                     MSKDAILHNYNGLLNYLTNPFVTRLEFANFHSSADGLKHFADVKWLFHLTQVVFLGLL
                     YPTLKTFTQRLKTKRFWLLQKPLILAALFPLMIGLMASFIGFEHFFTLFHQVLFVGDS
                     SWLFDPLKDPVIWILPEVFFLHCFLFFMIVYEIILWSLVGLARWQRLNVE"
BASE COUNT         6267 a         3579 c         4189 g         6152 t
ORIGIN      
        1 cagttttgtt tgtaatttca caaactattg catttctaaa aaaccaaata aggtaaggta
       61 gaaagcgatt taattatttg caaattgtga aaaaaagttt tatcataaaa aggtaatatg
      121 ataatatgga ggccgaaatg gtaacagttt ctaaagaaca acatttggat atgttcctta
      181 aaatggaacg tattcgtgaa tttgattcac gtattaataa actcgtccgt cgtggatttg
      241 tacaaggcat gactcacttc tcagttggtg aagaagcagc caatgttgga gcggtggctc
      301 acttgtctta cgatgacatt attttttcaa atcaccgtgg acatggacaa tcaatcgcta
      361 aagacatgga ccttaataaa atgatggctg agcttgctgg taaagcaact ggtgtttcta
      421 aagggcgtgg tggctcaatg cacttggctg attttgaaaa aggaaactat ggtaccaatg
      481 gtatcgttgg tggtggttac gctcttgcag ttggtgctgc cctcacccaa caatacaaag
      541 gaactaacaa tatagctgtt gctttctcag gtgatggcgc tactaacgaa ggatcattcc
      601 acgagtctgt taatatggct gcaacatgga aacttcctgt tattttcttc atcatcaaca
      661 accgttatgg aatttcaatg agcatcaata atgcaacaaa tacaccacac ttatacacac
      721 gtgcagaagc ttacggaatt cctggtttct actgtgaaga tggcaatgac gtgatggctg
      781 tctatgaaac aatgggcaaa gccgttgaac atgttcgtgg tggaaatggc ccagctattg
      841 ttgaggtaga atcataccgt tggtttggtc actcgactgc agatgctggt aaataccgta
      901 ctaaagaaga agtggatgag tggaaagaaa aagatccaat gatcaaatat cgtacttatc
      961 tgacaagcga aggcattgcc actgatgacg aattggatgc tattcaagca caagtcaaaa
     1021 aagaagttga cgatgcttat gaatttgcac aaaacagtcc agatccagaa ctttcagtag
     1081 ccttcgaaga cgtttgggtt gactaagatc accttgtcag taagaagcaa tcgtatttga
     1141 ataataaatg gagaaataaa aatgatgtca gaaacaaaat taatggcttt gcgtgaagca
     1201 gtcaaccttg ctatgactga ggaaatgcgc aaggacgaaa acattttcct tatgggagaa
     1261 gacgtagggg tttacggagg agacttcggg acttctgttg gaatgattga agaatttggt
     1321 cctaaacgtg ttaaagatac tccaatttca gaagctgcta tctctggtgc agctattggt
     1381 gcagcgatta caggactacg tccaattgtc gacgttacct tcatggattt ccttaccatc
     1441 atgatggacg ctatcgtgaa taatggtgct aaaaataatt acatgttcgg tggaggcctt
     1501 attacccctg taaccttccg tgtggcatca ggttctggta ttggttccgc tgcgcaacac
     1561 tcacaatctc ttgaagcatg gttgactcac attccaggaa tcaaagttgt tgctcctggt
     1621 aatgccaacg atgctaaagg cttattaaaa tcagctatcc gtgataataa cattgttctt
     1681 ttcatggaac caaaagcgct ttatggtaaa aaagaagaag ttaaccaaga tcctgacttc
     1741 tatatcccac ttggcaaagg tgacatcaaa cgtgaaggaa ctgatttgac aattgtgtca
     1801 tatggtcgta tgttggagcg tgtccttcaa gcggctgaag aagttgcagc ggatggtatt
     1861 aacgttgaag ttgttgaccc acgtaccctc atcccacttg ataaagagtt gattattgaa
     1921 tctgttaaga aaacaggtaa attgatgctt gtcaacgatg cctacaagac cggtggattc
     1981 attggggaaa ttgcaaccat gattaccgaa agtgaagctt ttgattacct tgatcatcca
     2041 atcgttcgtc ttgcaagtga agatgttcca gtaccttatg cacgtgtact tgaacaagcg
     2101 atcttaccag acgttgagaa aatcaaagct gccattgtga aaatggcaaa caaaggcaac
     2161 tagaagataa ggcaagtggt ctgataaagg ctatgtttat cagtaacgtc tcagctaaaa
     2221 actattttga aagtttttag aagaactgag ttgtcttatc ccgtgtcgcg gcacctcacc
     2281 tttcgtatgt tttcgtagca aggtgaagat gttgatggcg cttatcaaat cgtgaaagga
     2341 tgtagaaatg gcatttgaaa ttattatgcc aaagctgggc gttgacatgc aagaaggcga
     2401 gatcatcgag tggaaaaaac aagaaggtga taccgtcaat gaaggtgata tccttcttga
     2461 aatcatgtca gacaaaacca atatggaact tgaggcagaa gactcaggtg tgctattgaa
     2521 aattgtccgt ccagctggtg atactgtacc tgtaactgaa gttatcggtt acattggtgc
     2581 agagggtgaa tcagtagata ctattgcttc aagtgaaaaa acaacagaaa ttccagttcc
     2641 tgcttcagca gatgctggac caacagttgc tccaaaagaa aatgtggcaa gtccagctcc
     2701 tcaagtggca gcgacagcta ttccgcaagg aaatggcgga aaagttcgtg ctactccagc
     2761 tgcacgtaag gcagcagcag aaatgggcat tgaccttggc caagttccag gaacaggacc
     2821 aaaaggacgt gtccataaag aagacgttga aaactttaaa ggtgctcaac cgaaagcttc
     2881 accacttgct cgtaaaattg cagcagacaa aggtattgat ttggcaaccg tatcaggtac
     2941 tggctttaac ggtaaggtca tgaaagaaga catcaaggct attcttgaag cggctaaacc
     3001 agctgaagca aaagcaccag cagctaaaga agaaaaagtt gttgaccttc cagaaggtgt
     3061 ggaacacaag ccaatgtcag ctatgcgtaa ggcaatctct aaaggtatga caaactctta
     3121 ccttactgcg cctaccttta cgcttaacta cgatattgac atgacagaga tgattgccct
     3181 tcgtaagaag ttaattgacc caatcatggc taaaacaggc cttaaagtca gctttacaga
     3241 cttgattggt atggcagtgg ttaaaacctt gatgaaacct gagcatgagt acatgaatgc
     3301 ttccctcatc aatgatgcta atgatattga attgcaccgc tttgtgaacc ttggtattgc
     3361 cgtaggtctt gatgatggtc ttatcgttcc tgttatccat ggggctgaca agatgagctt
     3421 gtcagatttc gtgcttgctt caaaagatgt gattaaaaaa gcgcaaactg gtaagttgaa
     3481 agcagccgaa atgtctggat caaccttctc tatcacaaac ttgggaatgt ttggtacgaa
     3541 gacatttaac ccaattatta accaaccaaa ctcagcaatt cttggtgttg gagcaactat
     3601 cccaactcca acggttgtgg atggtgagat tgttgcccgt ccaattatgg caatgtgcct
     3661 aaccatcgac caccgtttag ttgatggtat gaacggtgca aaattcatgg ttgatctcaa
     3721 gaaattgatg gaaaatccat ttgaattgtt aatctgatca atcagtttta aacaagaaaa
     3781 gcattcaata ataaaaggga ttttgctaag tttagacttg gtgccctttt ctcaaaaata
     3841 tagaaaggat gtagaacgag gagctctcat gcaaatgagt gataagcaat tgaatccgag
     3901 cgaaaagctc ggataaaaat ggtaagagtt ggctaatctt agcgtcgtag acgtctgaac
     3961 tttcctcaat ttttgtttcg gaaagtccta gtcgtcctga cgtgggtgcg tcaacgaaat
     4021 caaagatttc tgacttaccg caaaaattca atcgctttct ccttgctctt ggtatcttaa
     4081 attatggctg ttgaaattat tatgccaaaa ctcggtgttg acatgcaaga aggtgaaatc
     4141 atcgagtgga aaaaacaaga aggtgatact gtcaatgaag gcgatattct tcttgaaatc
     4201 atgtcagata aaaccaatat ggaacttgaa gcagaagact caggtgttct tcttaaaatt
     4261 acacgtcaag caggtgaaac agtacctgta acagaagtta tcggctatat cggtgctgaa
     4321 ggtgaatctg ttgaggtttc tagcccagct gcttcagatg tgaatgttgc tcgtacaaca
     4381 gaagatttag aagctgctgg acttgaagtg ccaaaagcac cagctcaagc tgcttcagct
     4441 gcaccaaaag ctgcacttgc tgatgatgag tatgacatca tcgttgttgg tggtggccct
     4501 gctggttatt atgcagctat tcgtggtgct caacttggtg gtaaaattgc catcgttgag
     4561 aaatctgaat ttggtggaac ttgcttgaac gtagggtgta tcccaaccaa aacttacctt
     4621 aaaaatgctg aaatccttga tggtattaag attgcagcag gacgtgggat taaccttgcc
     4681 tcaactaact ataccattga catggacaaa acagttgact ttaaaaacac cgttgttaaa
     4741 accttaacag gcggcgttca aggtcttcta aaagctaata aagtcactat ctttaatggg
     4801 cttggtcaag tcaaccctga caagactgta accattgggt cacaaaccat taagggtcgc
     4861 aacgttatcc ttgctacagg ttctaaagta tcacgtatca atatcccagg tattgactct
     4921 aaacttgtct taacgtcaga tgatatcctt gaccttcgtg aaatgccaaa atcactagca
     4981 gttatgggcg gtggtgttgt tggtatcgag cttggacttg tttgggcatc ttacggtgtg
     5041 gatgttaccg ttattgaaat ggctgaccgt attatcccag ctatggataa agaagtctct
     5101 cttgaacttc aaaaaattct ttctaagaaa ggcatgaaga tcaaaacatc tgttggtgtc
     5161 tctgaaattg ttgaagcaaa taaccaattg accttgaaac ttaacaatgg tgaagaagtt
     5221 gtcgctgaaa aagctcttct ttctatcggt cgtgtaccac aaatgaacgg tctggaaaat
     5281 cttaaccttg aaatggatcg taaccgtatc aaagttaatg actaccaaga gacatcaatt
     5341 ccaggtatct atgcgccagg tgacgttaac ggaacgaaaa tgcttgctca cgctgcttac
     5401 cgtatgggtg aagtggctgc agaaaatgcg atacatggca acacaactcg taaagctaac
     5461 cttaaataca caccagcggc tgtttacaca caccctgaag tggcaatggt tggtttaact
     5521 gaagaacaag cgcgtgaaca atatggtgat gtgcttattg gtaaaaacag ctttactggt
     5581 aatggacgcg cgattgcttc aaatgaagca catggttttg ttaaagttat tgctgatgcc
     5641 aaataccacg aaatcttagg tgttcatatt attggtccag ctgcagctga gatgattaac
     5701 gaagcagcta ctattatgga atctgaatta accgttgatg aattgttatt atcaattcat
     5761 ggacacccaa ccttctctga agtgatgtac gaagcctttg cagatgtgct tggcgaagct
     5821 atccataacc caccaaaacg taagtaaaac gtctttgtct tattaactaa tgaaaaatat
     5881 catcacataa cagatcacaa aaaagactag cacaactatg aactgcaccc caaacgttag
     5941 atataaaact ctaacgtttg gggtgttttt gtatgacatt aagctatgaa gataagctag
     6001 acatttagcg ctaaaaaaac gagagaactc cgtttgaaag aagcatctct gaaaagatga
     6061 agacaaaaaa ccattataaa gaatggacaa agacgagctc tgattagact ttcttttaag
     6121 aacggctaaa ttgactcgtt ctacctacta ttatcatcga ttattgcaac aataaacgta
     6181 tcaaaataaa acgaaaagga cttagtcctc tacaacacag aactaaatcc tttacttcaa
     6241 atagagtagc caacttttgg ggctcagtgc agtttccagt ttttttgtct ttaataacgt
     6301 ttcattgtta acccttactt tccaataaca gctaaatcct taagtctttc ttacgaaagg
     6361 gttttgtgat atggttaaaa gaagcaacta aagctccacc taagaattct aaagaggagg
     6421 actgataccc agtatgcggt aagcgtcgtt gtcttttgtg gggaatttta tgagagttaa
     6481 gagcattgtt tttagctgca attccttgat gagcaagctt ttattgttga ttttgcttga
     6541 caggatgagg aaaactataa aagccttgat gagaaagatt atgatttgaa aacatcgttg
     6601 cttttttttc atagtgaatg acgtgcatct gttgactatc atgatcgtag acagcggcca
     6661 gtttgtcatt gttttctaac aagtcgatat caagcttgct aatataggtt tcaaattctt
     6721 gacgagtcat atttggaagc atcatatagg cataacgatc gccatcttga gtatggtttt
     6781 gcatgatagt gataaaggta tttgaaactt ctttgatggc ttctggtgat ttgtcatcag
     6841 cttttatgtt ttgccatttc cctgtttgaa gtgccttact tatgctaagt gttgttggct
     6901 tgaagaagta gtaaccaata ttttgagcgg gatcatcact ttcaaggaaa atacttttag
     6961 tgtttgtaaa atcaactagc tgattattca agtcaacggg ttgattgtta acataagaac
     7021 agtaagggtg cttttgattt tcttttcgtt gctcaatagt tgtatacgcc ttgtgagatg
     7081 actggttttt gatattgcta ccaacaaaga ttattttgtt tcctaagata aaccaccctt
     7141 tattgagagt gaggctttta ttccaattag tgaaggtcat agcagctaga gcacttgtgt
     7201 tattaagttt tttacttgca acaaaagcgt catcagagag actagtcatg ccaacttgtt
     7261 gatagttcgt tttaatattc tcaggagttc cctctagtgg tttttgctca gtttctgtgg
     7321 ttccaggtaa gcggtaggga tttaccgttg cccaatagtt ttcactgtag tgtcctaaat
     7381 cgttattgta taggtaaaac attccatcag aagtaaacca gccatgaaga ttttcattat
     7441 tcatagcttc ataattttga gttcgattcg aaaacattga tagaccaaaa gcaaaatcgt
     7501 gtttattatt atatagtgcc aatttatcca tactattgaa actagctacg taactatcaa
     7561 gtttttggac tggaacagaa gtatcactta gcagttcttt cataagtttg atatcgtgat
     7621 aggttttcaa attatcatag acattgtaaa aagcattccc ttgtgtgacg agtgttttta
     7681 tacgtgtttt aagtgccaaa cggtgaggct cttcagacat gtcagcaata cgtaaaatag
     7741 cacgaagtgc ttcaatacca gcaacatgag attgagcatt aaaacgactg atagaacgcc
     7801 ctcgagtcat atccatcatt tctccacgaa cgatgatagg gaaaaaagaa tggttaatcc
     7861 aatgatagat agtagccatt ttatccgctt ctataggaga ctttgttttt tgaataatag
     7921 gaattaattg cgataagcca tctataagaa cattaccgta agctccagtg taagcaatgc
     7981 cttttttata aagtggactt tgagcgttag taaccacgtg atcaattaaa gaaccgtctt
     8041 ggtaaaaacc atttccttcg tcaactagcg tgaaaacttt ctcaattgct ttgattgtat
     8101 cactaatttc gagatcatct ttacgaagaa taccggaaat gagtttaaca cgtcccatat
     8161 caattaaatt tccgctactg gcttcaaaag gtgaaaaatt ggcagcgcga accctaaaac
     8221 gagtagggtc aggcacaaat ttttcgattg gagctgtgta tttaaggatt tcttcttgag
     8281 taaaataagg atacatcaag gataaggtat tattaatagc acgaggagtt ccaatttcat
     8341 aaacccacca attttctttg ttttcttttc cagttgtttg atgattttca cgatttagat
     8401 tataagcgtg ttcatacatg aaggccatac cgtcttttac aatagcaata gctttactat
     8461 cttgataata gcatgattca ggattagtga tctgttttgc tattttttca atattgcggt
     8521 aagttttcgt gatattagta gaagcggaat aatcctttgc atgttcccaa aggtaagttc
     8581 tattttcgtg gtcaggccct tgatagcttt taataatgtt ttgagcatcc ttttcagctt
     8641 tattatgaaa agtgaccatg tcaggattgg ttttatcata agcatcgttg cctgcgataa
     8701 tactgttcca ttggtctaac aattcagtat agtagtcttt ttgttgtggc tgtactacct
     8761 ttttttcgct atctgtagta gtgagcgttt gcgtttgaaa gtaagtatta ttaggttctg
     8821 aatttgaagt cagtgtatcg gcataaatgg cagttccttg gcccatcata gcaaagctaa
     8881 ggaataggtt tgaataaagt agtaattgtt tatggtgtgt gcaaaaataa gtattcactg
     8941 ataaattcct ccaatataaa aatgagataa taaaagtaga ttggtttact aagtaaaaat
     9001 ataccacgtt aaagtatttt tataaaccct tacataaata atggctgagt ttttgtgcac
     9061 taataagtgt aggtactgaa gatgtcatac agaataggac acttgtcttt tatatttgtt
     9121 ttcattatac tagagaggta gattacattt ttgaaaggat tttttctcct atgaaataca
     9181 tcgttaacaa aagtcacaat cctgccttta acatcgcttt ggaagcctat gcttttagag
     9241 aactagtgga agaagacgaa ttgtttatcc tttggatcaa tgagccagct attattattg
     9301 gcaaacatca aaacaccatt caagaaatca ataaagaata cattgatgaa catggtattc
     9361 atgttgtgcg acgtttatct ggtggtggtg cggtttatca tgatttgaat aacttgaact
     9421 acactatcat ttcaaataaa acagcggaag gcgcatttga tttcaaaacc ttctcacagc
     9481 ctgtgattgc gactttagct gatttgggtg taacagccaa ctttacaggc cgtaatgata
     9541 ttgaaattga tggtaaaaaa atctgcggta atgctcaagc ttactacaaa ggtcgcatga
     9601 tgcaccatgg ttgccttttg tttgatgtgg atatgaccgt tcttggcgat gctcttaagg
     9661 taagtaagga taagattgag tctaaaggag tgaagtctgt tcgtgctcgc gtgaccaata
     9721 ttttaaatga attaccagaa aaaattaccg ttgaggaatt ttcagataaa atcttgacca
     9781 agatgaaaga aacgtaccca gacatgacag aatatgtctt gtcagaggat gagttggcta
     9841 agattgaaca atctgccaaa gagcaatttg gtagctggga ttggacctac ggcaaagcgc
     9901 cagaatatac gattgaacgc aatgttcgtt accctgcagg taaaatttca acctttgcaa
     9961 atgttgaaaa ttctatcatt aaaaacctta aaatctatgg tgatttcttt ggaatcaaag
    10021 atgtgcaaga cattgagaac ttattaattg gttgtaaata tgagtaccgt gatgtttttg
    10081 aacgtttaaa aacgattgat acgacccagt acttctcacg catgaccgtt gaagaagttg
    10141 ctaaagctat tgtttcttag tggttttaat cacttaagaa gctctgtcag aagaaaaaac
    10201 tataaaactc ctaagcgaaa tatcgtgtcc tgcttaggag tttttgtttg atgacatgaa
    10261 agaagaactt aagctttatc aaaggcagct ttacgtttaa tgtcggcata ttcttcagcg
    10321 acttcttgtt tcagtatatc atcatagcta ggtagtgtaa tagctgatcc gtacttttct
    10381 ttgagagctg ttgttacgag acggtaagca aggttcacgt tgcgtgataa gataggacca
    10441 tggaaataac tgccgtagac gtttttatag tgaacacctt cagtttggtc ttccttatta
    10501 ttaccattgc cataaaccac tcgtccaagt ggcttctcat catcgtataa gaaggtacga
    10561 ccttgatgat tttcaaagcc ataataggtt tcgttaaatt cgtcattatg aattttaata
    10621 tctccaataa agcgattttg gtgctgatta agggtgtagt gtcccataat tccaagtcca
    10681 tcaattttaa ccccattagc ttggacatag tattgcccca gtaattggaa gccaccacag
    10741 atggcaagca ccactttatt cttagcgata taatccgcta aagcagcttt tttgctaggc
    10801 aaatccttag caacgatggt ttgctcatag tcttgtccac caccaaaaaa aacaaggtcg
    10861 tagtaatctt ggtcaaaggc atcattgagg gaaacaatat caacggtaac tctagctcca
    10921 agtttttcag cgacatactt gagcatcaag acattcccat tgtcaccgta ggtattcatc
    10981 aggtttccgt agagatgagc aatgtttagg ttatagaggt agtcagtgtt ttctggagat
    11041 tttagtgaag tgtaagtcat tagttcatct ccttttcaac aacatggcgg tcggctagca
    11101 agctacggaa ttctaacatt gcagtatagg ttgctaaaat ataagcatgt ttgctttctt
    11161 gtttttcaat agcttccatg atttgctcga gcttttcagc ttgttttatc ttagtgtcgt
    11221 caaatcctgt gactctaagt cttctggcaa tttcagagtg acggacgccg ccagcattaa
    11281 tttctgtaat gggcatttgg gtaatgagtt caaaattagc atcccaaatc cagctggtat
    11341 caatgccgtc agcgtaatta gcatttaaaa gcacagataa gctaaatggg taatcagcta
    11401 gctgaatcat ttcaagcgct tgactggctc caacaggatt cttgataagg ataagggtgc
    11461 atgatttatc accaatagta aaagtttctt gtcttccaaa aaccgcctta cttttgttaa
    11521 agcccgcctt gattttctcg ggagaaacac cgaaaaattc agcaacagaa accgcagcaa
    11581 gcgcattgta aatattataa agaccaccaa catttatctt gtagttttgt ccatcaataa
    11641 caaattcaga actttggtga gtaatagcgg tgagttcagt taatcgataa tccaactgtg
    11701 gtctcttgaa gccacaattt aaacaaataa agtctcctag attcgcataa gtattgagac
    11761 gatactgaag aatatgttcg cattttggac ataaaatccc ttctgtgttg taatgagcta
    11821 gctgcggagc atgcttagca gtatcaaaac cataatactg aactggatta actatgtcct
    11881 tagaagagaa aataggacta tctccattgg ctagaatagt tgcttttgga gcatttcgag
    11941 caccatctac aatcatctga taagtagtat aaatctctcc gtagcggtcc atctgatccc
    12001 taaaaatatt agtataaaca aaaagactag gttttaagta agtggtaatt ctaggaagac
    12061 tagcttcgtc aatttctaaa acagcaattt gccttccaga tttccctttt ttagcagcta
    12121 aaaaagtaga agtaattcca gtaatcatat tggcaccact tggatttgtg attatctcgc
    12181 caaatgcctc tttcaaaata ccgacagtaa gagcagttgt taacgtttta ccattagtac
    12241 cagtgacgac cacaatatca tagtctttag acaaatcctt taaaatatct ttattacaag
    12301 ctagagccaa ttgaccagga taagtagatc ctcgccctag ttttgttaaa atggattgag
    12361 cagctttgcc agcaataatg ccgagtaatg tcttcatttt catgcttata ttttatcata
    12421 agtttattga ttttagatag ggaaaaatct ttgatcatga tatgatataa tatagctaat
    12481 aacttttttg atatagaaat gagagttgta tgaataattt atctagtatc gatattaaat
    12541 ttttattaag cttatttgcg gatccttggc tgctggcagt gcatctttta gatattctaa
    12601 ttgttgccta tttgatttat cgttttatta aagcattgac tggaactaaa atcatgtctt
    12661 tagttcaagg ggtcattttc tttttggttc ttcgttttat tgcagagtgg attggcttta
    12721 cgacaatcac atacttaatg aaccaagtca ttacttatgg tgtaattgca ggagtagtca
    12781 tttttacccc tgaaattcgg gcaggactgg agaaatttgg tcgttctacg caagtttttt
    12841 tacaaaaaca gtacgtcagc agtgaatctg ctttggtcga tgctttgatt aaatctgtgg
    12901 cctatatggg tccacgcaag ataggtgcac tgattgctat tgaacagact caaaccttac
    12961 aagaatatat tgcgacagga attccgctga atgccgatat ttcaagtcaa ctactcatca
    13021 atatttttat tccaaatacg ccgcttcatg atggagctgt tatcgttggt cagaataaga
    13081 ttgtggcagc ctgtgcttac ttgcctttgt cagaatcaaa ggcaatttcc aaagaatttg
    13141 ggacccgtca tagagcggct attggattat ctgaaaactc tgatgcacta actatcattg
    13201 tttcagaaga aacaggagct atttcagtaa ctcgcaaagg ccaattttta cacgatttaa
    13261 gcacagatga atttgaaacg gtacttagga cctacctgat gagtgattca aatgttagtt
    13321 taccatggta taagaaaatt ctaggaggga aatctaaatg aaacgatttt taaatagtcg
    13381 tccttggtta ggaatggtat cagtcttttt tgcgattctc ctttttttaa ctgcagcatc
    13441 gagcaatcat aataattcta gctcgcaaat ttatagcccg attgaaacct atacgcatag
    13501 ccttaaagat gtaccaattg atatgaagta tgatagtgac aaatatttta tcagtgggta
    13561 ttcttatggt gcggaagttt atctaacctc cactaatcgt atcaaattag attctgaagt
    13621 gaacaatgat acacgtaatt ttaagattgt ggcggatcta acacatagtc atccaggtac
    13681 agtaaatgtc aacctaagag tagaaaacct tccttctggg gtgacagcta ccgtatctcc
    13741 agataaaata tcagtcacaa ttggcaaaaa agaatctaag gtatttcctg ttagaggaag
    13801 cgttgatgct aaacaaattg caaatggtta tgaaattagc aaaattgaaa cgggggttaa
    13861 taaggttgaa gtgaccagtg atgaatctac cattgcttta attgatcacg ttgtggcaaa
    13921 gttacctgat gatcaagtgt tagataggaa ttatagtagt cgagtgacct tacaagctgt
    13981 ctcagctgat ggaactattt tagcaagtgc aattgatcct gcaaaaacaa atttatcagt
    14041 tgctgtaaaa aaaataacga aatcagtccc gattagagtt gaggcagtgg ggatgatgga
    14101 tgatagctta tcagatattc aatacaaatt gtcaaaacaa acagctgtta tctcaggtag
    14161 tcgagaggtc ttagaagata ttgatgaaat tattgcagaa gttaacattt ctgatgtcac
    14221 taaaaatact agtaagaccg tgagtttgtc ttctagtcag gtgtcaattg agccgtcagt
    14281 agtgaccgtt cagttgacaa ccaccaaaaa ataatggata ataactagga aaattgaatg
    14341 gaggaagcca ttaggcttat ataaattatg ggaaaatatt ttggaacaga tggtgttcgt
    14401 ggtgaagcaa atgttgagct gacacctgag ttagctttta aactgggccg attcggtggt
    14461 tatgttttaa gtcaacatga gaccgaaaga cctaaagttt ttgtggcgcg tgacacccgt
    14521 atttcaggag aaatgttgga gtcagccttg attgctggtc tactatctgt tggtattgaa
    14581 gtttataaac ttggtgtttt agcgacacct ggtgtctctt atttagttag aactgaaaaa
    14641 gcaagtgcag gtgtgatgat ttctgcaagt cataaccctg cgcttgacaa tggcatcaaa
    14701 ttctttggga atgacgggtt taagttagct gatgaccaag aattggaaat tgaagctctc
    14761 ttagatgctc cggaggacac gcttcctcgc ccgagtgcag aaggacttgg aaccttggta
    14821 gattatccag aaggacttcg taaatatgaa aagttcttgg taacaacagg tactgactta
    14881 agcggtatga cagttgcctt agacactgct aatggagctg cttcagtgtc agcacgtgat
    14941 gtctttttag atttaaatgc agaaattgct gtcattggcg aaaaaccaaa tggtcttaat
    15001 attaatgacg gtgttggttc aacgcatcca gaacaacttc aagagttagt taaggaaaca
    15061 ggagcagatc ttggtttagc ctttgacggc gatagcgacc gtctgattgc tgtggacgaa
    15121 actggtgaga ttgttgacgg tgatcgtatt atctttatca ttggtaaata cttgtcagaa
    15181 aaagggcttc ttgctcacaa taccattgtc acaacggtga tgtctaacct cggttttcat
    15241 aaagctttag ataaacaagg tattaataaa gcaattacag cagttggaga ccgctatgtg
    15301 gttgaagaaa tgcgtagctc aggatacaat ctcggtggtg aacaatcagg ccatgtcatt
    15361 atcatggact acaataccac tggagatggt cagttgacag ctatccaatt agccaaggtg
    15421 atgaaggaaa ctgggaaatc actttcggaa ttagctgctg aagtaaccat ttaccctcaa
    15481 aaactggtga atattcgtgt ggaaaacagc atgaaagacc gtgctatgga agttccagct
    15541 attgcgaaca tcattgcaaa aatggaggat gaaatggctg gtaatggtcg tattctcgtg
    15601 agaccaagtg gtacagaacc tcttcttcgt gtcatggcag aggcgccaac agatgccgaa
    15661 gttgattatt atgtcgatac tattgctgat gtcgtccgta cagaaatcgg atgcgataac
    15721 taacgagttg agtacaaaca agaaacctta tttctaaggc ttctgtcaac aattggaaag
    15781 aggcttgggg cgtttagccc aagtctcttt ttataaattg ctaaagtaga agagaaagga
    15841 tatcggatga caaaacagca aatcgaaaaa ctagcggact tactgcctcc ttttgcagcc
    15901 acactttact tagatatgat tcctgaattt caggaaggtt atgcttcata tgtttatcaa
    15961 acccaaaaag aagatattca aaagcgccgc atcaagcagt taactaaaaa ttttgaacga
    16021 ttactagata ccccatcttt ttatgtgatg cctgtgacaa gaaaaacagc agagcaattg
    16081 ctagcagata aaggcctagc ttatcatttg ttatcgacag ttgcagctaa tcaagtgttt
    16141 gcggctgctg ttaacgagga tcagacgctt tttcaagtgc ttcgaaatgg agcaagagta
    16201 gcctttgtga ttcttcaaga aaagggggaa tttctagaaa tgactttagg gattcttgaa
    16261 acagtgacag aaaacgaggt tccagcagct attgtggagg ctgtccagac atatcttctg
    16321 acagatatgc aagagaaact cccttcttta agcattatgg tcagtcaaga atcaagcaag
    16381 cttttttacc aagaacaagg gtttgttttg ctagctaagg gttgcaagcc aggaaacgaa
    16441 aatgactctg acgtttgtca gctcgtaaaa tacttttcca agccatgaag aatcaaggga
    16501 aatggtttat tcatagaaac attttatcat caaagtggta taataagacc atgtcaaaaa
    16561 aacctacttc agcctatgtg cacattcctt tttgcacaca aatttgttat tattgcgatt
    16621 tttcaaaagt ctttatccaa aatcaacctg ttgatgctta tttgaaagcc ttgattcaag
    16681 aattcgaatc ctatgacatt catgacttaa agactcttta tattggagga ggtacgccta
    16741 cagctatcac tgctaaacag ttggagtatc tcctaaatca ccttgaacga aacctcaatt
    16801 tagatgatct tgaagaattc acaattgagg caaatcctgg tgatttgacg ccagaaaaaa
    16861 ttgcggtgct tcagaggtca gctgtcaatc ggatttcctt gggggtgcag acttttaata
    16921 acaagcaatt aaagcagatt ggccgtagcc ataatgaaga acaaatttat agcaccattg
    16981 ccaatttaaa aactgcaggc tttcacaata tttccattga tttaatttat gccttgccag
    17041 gtcaaacctt agaacaagtc aaggaaaatg ttgctaaagc tttggcactt gatattcccc
    17101 atcttagtct ttatagcctt attttggaac atcacaccgt ttttatgaac aaaatgcggc
    17161 gtggtaaact taacttacca acggaagact tggaagcaga aatgtttgag tatattattt
    17221 cagaaatgga agctaatggt tttgagcatt atgaaatttc caattttacc aaaccaggtt
    17281 ttgaaagtcg ccataacttg atgtattggg ataatgtgga gtattttgga tgtggtgcag
    17341 gggcttcagg ttaccttaat ggcattcgtt atcaaaatcg ggtgcccatc cagcattatc
    17401 ttaaagcggt tgaagcagga aacgctagac taaatgaaga agtgctgcgc aaagaggaaa
    17461 tgatggaaga ggaactcttt ttgggcttac gtaaaaagac aggagtctcc atccagcggt
    17521 tccaagaaaa atttggtatt tcttttgagg agcgttatgg aaatattgtt agggagcttc
    17581 aaaatcaagg actgcttgtc aaagatgacg cctttgtcag aatgactaaa aagggtcttt
    17641 tcttaggtga ttctgttgct gagcggttta tattagatta ggagagtatt atgggattaa
    17701 gttatcagga ggagttgaca cttccttttg aattatgtga tgtcaaatca gatataaaat
    17761 tgcccctttt attagactat tgtttgatgg tttctggtag acagtctgcg caattaggac
    17821 gaagtaacaa caatctttta gttgattaca agcttgtttg gattgtaacg gattatgaga
    17881 tcactattca tcgcttgcca cattttcaag aaaccatcac cattgaaaca aaagcccttt
    17941 cctataataa atttttttgt tatcgccaat tttatattta tgatcaagag gggggtcttt
    18001 tagtggatat cttagcctat tttgctttgt taaacccaga tacgcgaaaa gtggcaacta
    18061 ttccagaaga tttagtagcg ccttttaaga ctgattttgt taaaaagcta caccgtgttc
    18121 ctaaaatgcc tcttttagaa caatcaattg atcgtgatta ttatgtgcgt tattttgata
    18181 ttgatatgaa tggtcatgtc aacaacagta aatatttaga ttggatgtat gatgtgttgg
    18241 ggtgtgcgtt tttaaaaacg catcagcctc ttaagatgac tttgaaatat gttaaagaag
    18301 tctcaccagg cggtcaaatt acttccagtt accatttgga ccaattaact tcttaccatc
    18361 aaatcatctc agatgggcag ctgaatgccc aagccatgat tgaatggcga gcgattaaac
    18421 aaacagaaag cgagatagac tagtgcctta taagggttat ttaattgatt tagacggaac
    18481 gatttaccaa gggaaaaacc gtattccagc aggggagcgg tttatcaaac gcctacaaga
    18541 aaaaggcatt ccctatttat tagtgactaa taataccaca agaacacccg aaatggtgca
    18601 aagcatgctg gctaaacagt ttaatgttga gacaggcatt gagactatct acacagctac
    18661 catggcaacc gttgattaca tgaatgacat gaaccgtggc aagacagctt acgttattgg
    18721 agaaacaggg ctcaaatcag ctattgcagc agcaggttac atagaagaac ttgagaatcc
    18781 cgcttacgtg gtggttggtt tagacagtca ggtgacctat gagatgttgg ctatagccac
    18841 cttagctatt caaaaaggtg ccctctttat tgggacaaat cctgatttaa acattccaac
    18901 cgaacgtggt ttgatgccag gagcaggtgc attgaatgcc ttactagaag ctgccacacg
    18961 tgtaaaacct gtttttattg gcaagcccaa cgctatcatt atgaataaat cactagaggt
    19021 attgggcatt cagcgctctg aagccgtcat ggttggcgat aattacttga ctgatatcat
    19081 ggcaggtatt caaaatgata ttgcaacgat cctcgtgaca acaggcttta ccagacctga
    19141 agaggtacca accttaccaa ttcagccaga tcatgtccta tctagtcttg atgagtggag
    19201 gttgtgatgg tagaaaacac taaattacta tgtagctggg tatggttatt agcgttagct
    19261 attctcataa cgatctattc aacgtggctg tggtatcctc ttgaggtgga ccatctaaag
    19321 ttagaacaag ttgtttttat gagcaaggat gctatcttgc ataactataa cggactacta
    19381 aattacctca ccaatccttt tgtaacaagg ttggaatttg ctaattttca ctcatcggca
    19441 gacggactca agcattttgc agatgtgaag tggctttttc acctaactca agtcgttttt
    19501 cttggcctcc tttacccaac ccttaagact ttcacacaaa ggttaaaaac caaacgtttc
    19561 tggctccttc aaaaaccttt aatactagca gctttgtttc ctcttatgat tggtctaatg
    19621 gctagtttca ttgggtttga acattttttt actctttttc atcaggtgct ttttgtagga
    19681 gatagcagct ggctctttga tcccttgaaa gatcctgtga tttggatcct tccagaagtc
    19741 tttttcttgc actgtttcct tttctttatg atagtttacg aaatcatcct gtggagctta
    19801 gtaggtttag caagatggca gcgcctaaat gtagaatgat aaaacaagcg ttttgaaagc
    19861 gcttgttttt tagtttgcag tcagagtaga atagaagtat caaaaaaaag caccgactcg
    19921 gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat gctgttttga
    19981 atggttccaa caagattatt ttataacttt tataacaaat aatcaaggag aaattcaaag
    20041 aaatttatca gccataaaac aatacttaat actatagaat gataacaaaa taaactactt
    20101 tttaaaagaa ttttgtgtta taatctattt attattaagt attgggtaat attttttgaa
    20161 gagatatttt gaaaaagaaa aattaaa
//
LOCUS       DAUDYX010000038        20150 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.038, whole genome shotgun sequence.
ACCESSION   DAUDYX010000038 DAUDYX010000000
VERSION     DAUDYX010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20150
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.038"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            5..78
                     /locus_tag="VHD02_001343"
     tRNA            5..78
                     /locus_tag="VHD02_001343"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Asn,seq:gtt)
     gene            158..231
                     /locus_tag="VHD02_001344"
     tRNA            158..231
                     /locus_tag="VHD02_001344"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:192..194,aa:Arg,seq:acg)
     gene            227..808
                     /locus_tag="VHD02_001345"
     CDS             227..808
                     /locus_tag="VHD02_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEQ5497949.1"
                     /translation="MHNEKSNRTLVSCCFFVVLFILGIVKKKRRLKMSIETRAAFEKV
                     KPIILKLKRHYYIQLWDRDDWLQEGHIILLQLLERYPELIEEEERLYRYFKTKFSSYL
                     KDLLRRQESQKRQFHKLAYEEIGEVAHAIPSRGLWLDDYVAYQEVIASLENQLNSQER
                     MQFQALIRGERFKGRRALLRKISPYFKEFAQQL"
     gene            1367..1894
                     /locus_tag="VHD02_001346"
     CDS             1367..1894
                     /locus_tag="VHD02_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEQ5497950.1"
                     /translation="MSIDDYRPTYMVEAIYDLRANDLLRHGITAVLVDLDNTLIAWNN
                     PDGTPEVRAWLDEMTIADISVVVVSNNKHSRVERAVSRFGVDFISRALKPFAYGIEKA
                     IARYGFDRNEVIMVGDQLMTDIRASHRAGIKSVLVKPLVASDAWNTKINRWRERRVMA
                     KLEEKYGKLSYQKGI"
     gene            1894..3012
                     /gene="yqeH"
                     /locus_tag="VHD02_001347"
     CDS             1894..3012
                     /gene="yqeH"
                     /locus_tag="VHD02_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEQ5497951.1"
                     /translation="MEELFCIGCGIQIQTEDKEKAGFTPAAALKKGMETGELYCQRCF
                     RLRHYNEITDVHITDDEFLRLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFISGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPLDVMLTSAQNKYAIKDLIERINEL
                     RNGRDVYVVGVTNVGKSTLINAIIQEITGNKDVITTSRFPGTTLDKIEIPLDDGTFIF
                     DTPGIIHRHQMAHYLSPKELKIVSPKKEIKPKTYQLNPEQTLFLGGLSRFDFINGERQ
                     GFTAFFDNQLELHRTKLAGADAFYDKHVGTLLTPPDKKELTAFPKLVRHEFTIDQKMD
                     IVFSGLGWIRVNGQKDSKAIVAAWAPEGVAVIVRKAII"
     gene            3037..3345
                     /gene="yhbY"
                     /locus_tag="VHD02_001348"
     CDS             3037..3345
                     /gene="yhbY"
                     /locus_tag="VHD02_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEQ5497952.1"
                     /translation="MLTSKQRAFLKSEAHSLKPIVQIGKNGLNDHIKTSIRQALDARE
                     LIKVTLLQNTDEDIHEVAEILEEEIGCDTVLKIGRILILYKVSAKKENRKLSPKVKAI
                     "
     gene            3414..4046
                     /locus_tag="VHD02_001349"
     CDS             3414..4046
                     /locus_tag="VHD02_001349"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEQ5497953.1"
                     /translation="MALELLTPFTKVELEEEKKESNRKQIGILGGNFNPIHNAHLVVA
                     DQVRQQLGLDQVLLMPECKPPHVDAKETIDEKHRLRMLELAIEDVEGLAIETCELERQ
                     GISYTYDTMLYLTEQHPDVDFYFIIGADMVDYLPKWHRIDELVKLVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRDFIKKGRQPNYLLPKRVLDYITQEGLYQ"
     gene            4043..4636
                     /gene="yqeK"
                     /locus_tag="VHD02_001350"
     CDS             4043..4636
                     /gene="yqeK"
                     /locus_tag="VHD02_001350"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEQ5497954.1"
                     /translation="MTYEDYLPYSRTELLAKIAEQMSPKRFKHVLGVEKAALSLAECY
                     GCNPDKAGLAALLHDYAKECPDQVFLDLIDKYQLSPELAKWNNNVWHGMVGIYKIQED
                     LGLKDKDILRAIEIHTVGAAEMTLLDKVLYVADYIEEGRIFPLVDDARKIAKLDLNQA
                     VAYETVNTVAYLASKAQPIFPQTLDTYNAFCSYLKEY"
     gene            4660..5013
                     /gene="rsfS"
                     /locus_tag="VHD02_001351"
     CDS             4660..5013
                     /gene="rsfS"
                     /locus_tag="VHD02_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEQ5497955.1"
                     /translation="MKKEELLKIVVEATDEKRAKDILALDLEGLTSLTDYFVIASATN
                     SRQLEAIADNIREKVKETGGDASHVEGNSQAGWVLLDLTDVVVHLFLEDERYHYNLEK
                     LWHEAPAVALDAYLA"
     gene            5061..5804
                     /locus_tag="VHD02_001352"
     CDS             5061..5804
                     /locus_tag="VHD02_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ5497956.1"
                     /translation="MEHNYEKFASVYDAVMDDSLYDLWTDFSLRHLPKSKGRNRLLEL
                     ACGTGIQSVRFAQAGFDVTGLDLSQDMLAIAKKRAQSAKKKIDFIQGNMLDLSQVGQF
                     DFVTCYSDSICYMQDEVDVGDVFKEVYDVLANDGIFIFDVHSTYQTDECFPGYSYHEN
                     ADDFAMVWDTYADEVPHSVVHELTFFIQEDDGRFSRFDEVHEERTYELLTYDILLEQA
                     GFKSFKLYADFEDKEPTETSKRWFFVAYK"
     gene            6026..7132
                     /locus_tag="VHD02_001353"
     CDS             6026..7132
                     /locus_tag="VHD02_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEQ5497957.1"
                     /translation="MTVTGIIAEFNPFHNGHKYLLETAEGLKIIAMSGNFMQRGEPAL
                     IDKWIRSEMALKNGADIVVELPFFVSVQSADYFAQGAIDILCQLGIQQLAFGTENVID
                     YQKLIKVYEKKSEQMTAYLSTLEDTLSYPQKTQKMWEIFAGVKFSGQTPNHILGLSYA
                     KASAGKHIQLCPIKRQGAAYHSKDKNHLLASASAIRQHLNDWDFISHSVPNAGLLINN
                     PHMSWDHYFSFLKYQILNHSDLTSIFQVNDELASRIKKAIKVSQNIDHLVDTVATKRY
                     TKARVRRILTYILVNAKEPTLPKGIHILGFTSKGQAHLKKLKKSRPLITRIGAETWDE
                     MTQKADSIYQLGHQDIPEQSFGRIPIIIKNERLN"
     gene            7614..8330
                     /locus_tag="VHD02_001354"
     CDS             7614..8330
                     /locus_tag="VHD02_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ5497958.1"
                     /translation="MGRKWANIVAKKTAKDGATSKVYAKFGVEIYVAAKQGEPDPELN
                     TALKFVIDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADSVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            8533..9375
                     /locus_tag="VHD02_001355"
     CDS             8533..9375
                     /locus_tag="VHD02_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ5497959.1"
                     /translation="MIKKQYLGRATIALASTLVLAACGSSKTAESGNQGSSKEVLFAT
                     VGTTAPFSYEKGGQLTGYDIEVAKAVFKGSDDYRVSFKKTEWSSIFTGLDSGKYQMGG
                     NNISFTKERSAKYLFSYPIGSTPSVLVVPKDSDIKSFDDIQGHTTQVVQGTTSVAQLE
                     DFNKKHSDNPVTLKFTNENITQMLTNLSEGKADFKIFDAPTVNAIIKNQGLDNLKTIE
                     LTSTEQPFIYFIFSQDQEKLQSFVNKRIKELTADGTLSKLAKEHLGGDYVPSDKELKL
                     PTAN"
     gene            9702..10547
                     /locus_tag="VHD02_001356"
     CDS             9702..10547
                     /locus_tag="VHD02_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEQ5497960.1"
                     /translation="MSFKKWIGIVGIALASTVLVACSEKQDDKNTLTIGVMTKTESDQ
                     ARWDKVEELLKKDNITLKYKEFTDYSQPNKAVANGEVDINAFQHYNFLNNWNKENKEH
                     LVAIADTYISPINLFSGTSQDGKAKYKSVADLPNGAQIAVPNDATNESRALYVLQSAG
                     LIKLNVSGDQLATIANISENKKKLDIKELDASQTARALVSADAAVVNNSYAVPAKIDY
                     KTSLFKEKADDNSKQWINIIAGQKDWEKSEKADAIKKLIKAYQTDEVKKVVEKTSNGI
                     DVSVW"
     gene            10802..11866
                     /locus_tag="VHD02_001357"
     CDS             10802..11866
                     /locus_tag="VHD02_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEQ5497961.1"
                     /translation="MNEAIIQLDHIDITFRQKKRVIEAVKDVTVHINQGDIYGIVGYS
                     GAGKSTLVRVINLLQAPTNGKITVDGDVTFDQGKVQLSANALRQKRRDIGMIFQHFNL
                     MAQKTAKENVAFALRHSSLSKTEKEHKVIELLELVGLSERADNYPAQLSGGQKQRVAI
                     ARALANDPKILISDEATSALDPKTTKQILALLQELNRKLGLTIVMITHEMQIVKDICN
                     RVAVMQNGVLIEEGSVLDIFSNPKEALTQEFITTATGIDEALEKINQQDIVKHLPANA
                     LLAQLKYAGTSTDEPLLNSIYRQFEVTANILYGNIEILDHIPFGDMIVVLEGQAENIL
                     AAEKALHEAGVDVSILKRGA"
     gene            11867..12559
                     /locus_tag="VHD02_001358"
     CDS             11867..12559
                     /locus_tag="VHD02_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ5497962.1"
                     /translation="MSQLIQTYLPNVYELGWSGDAGWGLAIWNTLYMTIVPFIVGGAI
                     GLLLGLLLVLTGPDGVIENKTICWVIDKVTSVFRAIPFVILIAILASFTYLLLRTTLG
                     ATAALVPLTFATFPFYARQVQVVFSELDKGVIEAAQASGATFWDIVKVYLSEGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGNVAISYGYNRFNNDVTWVATIIILLIIFAIQF
                     IGDSLTRRFSHK"
     gene            complement(12613..13983)
                     /gene="brnQ"
                     /locus_tag="VHD02_001359"
     CDS             complement(12613..13983)
                     /gene="brnQ"
                     /locus_tag="VHD02_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEQ5497963.1"
                     /translation="MKQKNVYIVIGFMLFALFFGAANLIYPAFLGIYSGHQILWSIIG
                     FCLTGVSLPLLGVIAVAKSGSGDVESLARPISKWYAIFYSSILYLSIGPFFAIPRTGA
                     TSFSVGIAPILGDNTTNKAIYAILFFGLSYFLAIKPSKLAENIGKFLTPTLLVVISIL
                     VIASFVHPAGNYGDAFNAGVGVNNAFKDFPFIAGLIQGYGTMDALASLVFAILVIEAT
                     KQFGAKTDKEVTKITLISGAIAILLLALVYIFVGRIGATSQSLFPFIDGSFTLHGNPV
                     NGGQILSHASRFYLGGIGQAFLAVVIFLACLTTSTGLITSSAEYFHKLVPALSHIAWA
                     TIFTLLSAFFYFGGLSVIINWSAPVLFLLYPLTVDLIFLVLAQKCFNNDPIVYRTTIG
                     LTFIPAIFDALLTLSQMTGLFHLPEAVVTFFQKTVPLGQFSMGWIIFAAIGFLIGLIL
                     SKTKKS"
     gene            14217..15431
                     /gene="sstT"
                     /locus_tag="VHD02_001360"
     CDS             14217..15431
                     /gene="sstT"
                     /locus_tag="VHD02_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEQ5497964.1"
                     /translation="MKKIYDLWVRVSLIKKIGIGVVIGVMLGILAPDLTGFSILGKLF
                     VGGLKAIAPLLVFALVSQAISHQKKGKQTNMTLIIVLYLFGTFASALVAVLTAYLFPL
                     TLVLNTPVNTELSPPQGVAEVFQSLLLKLVDNPINALATANYIGVLSWAIIFGLALKA
                     ASQETKHLIKTAAEVTSQIVVWIINLAPIGIMSLVFTTISENGVGILSDYAFLILVLV
                     GTMVFVALVVNPLIAVLITRQNPYPLVLRCLRESGLTAFFTRSSAANIPVNMQLCQKI
                     GLSKDTYSVSIPLGATINMGGAAITINVLTLAAVHTFGIPIDFLTALLLSVVAAVSAC
                     GASGVAGGSLLLIPVACSLFGISNDLAMQVVGVGFIVGVIQDSCETALNSSTDVLFTA
                     IAENAFWKRKKA"
     gene            complement(15486..16160)
                     /locus_tag="VHD02_001361"
     CDS             complement(15486..16160)
                     /locus_tag="VHD02_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEQ5497965.1"
                     /translation="MLKRKTVGVLGLGIFGRTVARELSNFDQDVIAIDIRESHVKEVA
                     DLVTKAAVGDITDKEFLLAVGIEHCDTVVIASGNNLESSVLAVMHCKKLGVPTIIAKA
                     KNKIFEEVLYGIGATKVITPERDSGKRVASNLLRRHIESIIYLEHGISMIEFVIPKSW
                     EGQSLSELDVRRKYELNVIGMRQKEVKTLDTNVKPFEPLEPNTIIVAIANDHTFEKFD
                     YLGYLK"
     gene            complement(16170..17561)
                     /locus_tag="VHD02_001362"
     CDS             complement(16170..17561)
                     /locus_tag="VHD02_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEQ5497966.1"
                     /translation="MSGGNMKRSFIKSLSVTQRLTFSFAIVILIGTLLLSMPFTHYQN
                     GPNTVYLDHFFNVVSMVCVTGLSVVPVAEVYNGIGQTIAMALMQIGGLGLVTLIAVST
                     FALKRKMRLSDQTLLQSALNRGDSKDLKHYLFFAYKVTFSIEAFAAIVIMIDFIPRFG
                     WKNGIFNSIFLAVSAFCNAGFDNLGSSSLKDFMLNPTLNVIITFLIISGGLGFAVWVD
                     LGVAFKKYFFERPHCYGATFRKLSNQSRLVLQTTAVILFLGTFLTWFLEKDNSKTIAN
                     FSLHQQLMVSFFQTVTMRTAGFATISYNDTLAPTNILYMIQMVIGGAPGGTAGGIKVT
                     TAAITFLLFKAELSGQSEVTFRNRIIANKTIKQTMTVLIFFFAVLMIGFILLLSVEPH
                     IAPIPLLFESISAIATVGVSMDLTPQLSTAGRLIVIVLMFVGRVGPITVLISLIQRKE
                     KTIQYATTDILVG"
     gene            complement(17631..18344)
                     /gene="rsmG"
                     /locus_tag="VHD02_001363"
     CDS             complement(17631..18344)
                     /gene="rsmG"
                     /locus_tag="VHD02_001363"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEQ5497967.1"
                     /translation="MTPQDFYRTLEEDGFSLSSKQKEQFDTYFKLLVEWNTKINLTAI
                     TEENEVYLKHFYDSIAPILQAFLANEPIKLLDIGAGAGFPSLPMKILFPNLEVTIIDS
                     LNKRISFLTLLAQELGLENVHFFHGRAEDFGQDKAFRGQFDVVTARAVARMQVLSELT
                     IPFLKIGGKLIALKAQAADQELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKK
                     KETPNKYPRKAGLPNKKPL"
     gene            18494..19051
                     /locus_tag="VHD02_001364"
     CDS             18494..19051
                     /locus_tag="VHD02_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEQ5497968.1"
                     /translation="MPTVLIILVVLGVLALWLMISYNSLVKSRMHTKEAWSQIDVQLK
                     RRNDLIPNLIETVKGYASYEQKTFEKITDLRARVANASTPQETMAASNELSKQVTSLF
                     AVAENYPDLKANENFLKLQEELTNTENKISYSRQLYNSTTSNYNLQLESFPSNIAGKL
                     FGFKPSEFLQTPEAEKEVPKVEFNF"
     gene            19098..19994
                     /gene="htpX"
                     /locus_tag="VHD02_001365"
     CDS             19098..19994
                     /gene="htpX"
                     /locus_tag="VHD02_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEQ5497969.1"
                     /translation="MLYQQISQNKQRTVVLLVVFFALLALIGASAGYLLLDNYAMGLV
                     LALVIGVIYATSMIFQSTSLVMSMNNAREVTEKEAPGFFHIVEDMAMVAQIPMPRVFI
                     IEDPSLNAFATGSSPQNAAVAATTGLLEVMNREELEGVIGHEISHIRNYDIRISTIAV
                     ALASAVTVISSIGGRMLWYGGGSRRQRDDGDDDVLRIITLLLSLLSLLLAPLVASLIQ
                     LAISRQREYLADASSVELTRNPQGMIKALEKLQLSQPMKHPVDDASAALYINEPRKKR
                     SFSSLFSTHPPIEERIERLKNM"
BASE COUNT         6545 a         3285 c         4145 g         6175 t
ORIGIN      
        1 tttatccgcc atagctcagt tggtagtagc gcatgactgt taatcatgat gtcgtaggtt
       61 cgagtcctac tggcggagta gagagaggta ggaagcgtcc tatctttttt tgcattttag
      121 agaagagtgt ggtaagatag aggcaagata tgcgttggca cccttagctc aactggatag
      181 agtacctgac tacgaatcag gcggttagag gttcgactcc tctagggtgc ataatgaaaa
      241 aagcaacagg acattagtgt cctgttgttt ttttgtggtt ctctttatac tagggatagt
      301 taaaaagaaa aggagactca aaatgtcgat agagacaaga gcagcgtttg aaaaagttaa
      361 gcccattatt ttgaaattaa agcgacacta ttatattcaa ttgtgggata gagatgactg
      421 gttacaagaa ggacatatta tcttattaca gttactagaa aggtacccag agttaattga
      481 ggaagaagag cgtttatatc gctattttaa aacaaaattt tcatcttatt tgaaagattt
      541 attacgccgt caagaaagtc aaaagcgtca gttccataag ttagcatatg aagagatagg
      601 ggaggttgca catgccattc catcgagagg gttatggcta gatgactatg tggcttatca
      661 agaggtaata gctagcttag agaaccaatt aaattcgcaa gagcgtatgc agtttcaagc
      721 acttatcagg ggcgaacgtt tcaagggaag acgtgctcta cttaggaaga tcagtcccta
      781 ttttaaggaa tttgcacagc agttgtaagc aagacctata aggaattgga ggtaaatcta
      841 tgaaaaaagt gtaatcattt tttgatttac agttattatc atgtgttatc tggcgactgt
      901 cctgtttaca agtaagttca caagtatggg ataaaatagt ataaactata gtaaatactg
      961 aatagtcaaa gaaaaatatc ggcataggtc agaagagctg atatgtttaa tactttttgc
     1021 ggatatctac tgtatttaaa tgtatttgat atccttgtca aggatatacg tctaaacaag
     1081 ttcaaaacga aatcatttgt gattatctta tcaggagttg actcttgata agatttttgt
     1141 atatcagtac tgtggtacgt ttagatagct tggcagtgaa tttatagagt gtcaaacaac
     1201 gagtgttttg tgttacacta accattagaa gagtctattg ttgagcatat caaggtgtta
     1261 tcttttcgag ttgaggtatc atatctagta tatttaacta tggttttaaa tgctatttca
     1321 aaaaaaatat gataaaatag aaagtactaa ttattttgga gatgacatga gtatcgatga
     1381 ttataggccg acctatatgg tagaggctat ttatgattta agagcaaatg atttgttgcg
     1441 tcatggtatt acggctgttc tagtggattt agataatacc ttaattgcat ggaataatcc
     1501 tgatggcaca ccagaggttc gcgcttggct tgatgaaatg actatcgcgg atattagcgt
     1561 ggttgttgtt tcaaataata aacattctcg tgttgagcgt gccgtttcac gctttggtgt
     1621 tgactttatt agccgggctt tgaagccatt tgcttatggt attgaaaaag ccattgctcg
     1681 ttatggattt gatcgcaacg aggttatcat ggttggagat cagctaatga ctgatatcag
     1741 agctagtcat agagctggaa taaaatcagt tttagtcaaa ccacttgttg cttctgatgc
     1801 ttggaatact aaaattaacc gctggcgtga acgccgtgtg atggcaaaat tagaagagaa
     1861 atatggtaaa ttatcatatc aaaaaggaat ttaatggaag aattattttg tataggctgt
     1921 ggtatccaga ttcaaactga agataaagaa aaagctggtt ttacgccagc tgctgctttg
     1981 aaaaagggta tggaaacagg agaactctat tgtcagcgct gctttagatt gaggcattat
     2041 aatgaaatta cagatgtcca tattaccgat gatgaatttt taagacttct tcacgaagtg
     2101 ggtgatagtg atgctcttgt ggttaatgtg attgatattt ttgactttaa tggttctatt
     2161 attccaggtc tatctcgctt tatttctggt aatgatgtct tactggttgg gaataaaaaa
     2221 gatattttgc caaagtctgt taaggatggt aaagttactc aatggttgac ggaacgtgca
     2281 cacgaagaag gtcttcgccc tttagacgtt atgttaacaa gcgctcaaaa taaatatgcg
     2341 atcaaagact tgattgagcg tatcaatgag ttacgtaatg gtcgagatgt ttatgttgtg
     2401 ggggtcacta atgttggtaa atcaaccttg attaatgcga ttatccaaga gattactggt
     2461 aacaaggatg tcatcacgac gtctcgtttt cctgggacaa ctcttgacaa aattgaaatt
     2521 cctttggatg atgggacgtt catttttgat acaccaggaa ttattcatag gcaccagatg
     2581 gctcattatt tatcccctaa agagctaaaa atagtgagtc ctaagaagga aatcaagcct
     2641 aagacgtatc aattaaaccc agaacaaact ctctttttag gtggtttgtc tcgctttgac
     2701 tttatcaatg gggagcgtca agggtttaca gctttttttg acaatcaact agaattacat
     2761 cgtacaaaat tagctggagc agatgctttt tatgataagc atgttggaac tttgttaacc
     2821 ccgccagata agaaagagtt gactgcattt cctaaattag ttcgtcatga atttacaatt
     2881 gatcagaaaa tggatattgt tttttctggt ctaggatgga ttcgtgttaa tggtcaaaaa
     2941 gactcaaaag cgatagttgc tgcatgggct cctgaagggg tcgctgtcat tgttcgtaaa
     3001 gcgattattt aaaaataaaa aagaagttgg aaaaaaatgc ttacaagtaa acaaagagct
     3061 ttcttaaaat ctgaggctca tagcttaaaa ccgattgtac aaattggcaa aaatggatta
     3121 aatgaccaca ttaagactag tattcgtcaa gctttggatg ctagagaatt gataaaggtc
     3181 actttgttgc aaaatactga tgaagatatt catgaagtag cagagatttt agaagaagaa
     3241 attgggtgtg atacggtttt gaaaattggt cgtatcttga ttctctataa ggtttctgct
     3301 aagaaagaaa accgcaagtt gtcccctaaa gttaaagcaa tctagtcctt ttatttgtat
     3361 agggactggt tttatgagac gaggactatt gtcgcgagaa ttttggagaa agtatggcat
     3421 tagaattgtt gacgcctttt acgaaggtgg aattagagga agaaaaaaaa gaaagcaacc
     3481 gcaaacagat tgggatttta ggagggaatt ttaatcctat tcacaatgca caccttgtgg
     3541 ttgcagatca agttcgccag caattaggat tagatcaggt gctattgatg cctgaatgta
     3601 aaccaccgca tgtggatgcc aaagagacta ttgatgagaa gcatcgtctt cgcatgttgg
     3661 aattggctat tgaagatgtt gaagggctag ctattgaaac ctgtgaactt gaacgtcagg
     3721 gaatcagcta tacctatgat acgatgcttt atttgactga gcagcatcct gatgttgatt
     3781 tttattttat catcggagct gatatggttg attatttgcc aaagtggcac cgtattgatg
     3841 aactggtcaa actcgttcaa tttgttggag ttcaacgtcc aaaatataaa gctggaacct
     3901 cttacccggt catttgggta gatcttcctt tgatggatat ttcttctagt atgattaggg
     3961 attttattaa aaaaggtcgt cagcccaatt atctcttacc taagcgggtt ttagactaca
     4021 ttactcaaga aggtttgtat caatgacata tgaagattat cttccttata gcaggaccga
     4081 actgttagca aaaattgcag agcagatgag ccctaaacgg tttaaacatg tacttggcgt
     4141 ggaaaaggca gccttgtcct tggcagagtg ttatggttgt aacccagaca aagcagggtt
     4201 agcagctctt ttacatgatt atgccaagga gtgccctgac caggtctttt tagacttaat
     4261 tgacaagtac caattatctc cagaattagc taaatggaat aataatgtct ggcatggtat
     4321 ggttggtatt tataagattc aagaagacct agggctaaaa gacaaagata tcttgcgagc
     4381 tattgaaatt cacaccgttg gcgctgctga gatgaccttg ctggataagg tcttatacgt
     4441 tgctgattat attgaagaag gtcgcatttt tcctttggtt gatgacgcta gaaaaatcgc
     4501 taagctcgat ttgaatcagg ctgttgccta tgaaacagtg aacactgtcg cttatttagc
     4561 ctcaaaagcg cagcctattt tcccacaaac tctggatacc tataatgcgt tttgcagcta
     4621 tttaaaggaa tactaatgga taatgatatg aggaataaaa tgaaaaaaga agaactctta
     4681 aaaattgttg tggaagcaac tgatgaaaag cgtgctaaag atattttagc cttggattta
     4741 gaggggttaa ctagtttgac cgattatttt gtgattgcga gtgcaacaaa tagtcgtcaa
     4801 ttagaggcta ttgcggataa tattcgtgaa aaggtaaaag agactggtgg agatgctagc
     4861 catgttgaag gtaatagcca agcaggctgg gtcttgcttg atttgaccga tgtggtggta
     4921 catctttttt tagaagacga gcgttaccac tataatcttg aaaaattgtg gcatgaagcg
     4981 cctgctgtgg ctctagatgc ctatttagct taatgttaca aagagacctt gtcaatacga
     5041 cagggttttt gaggatgata atggaacata attatgaaaa atttgcgtca gtttatgatg
     5101 ctgtgatgga tgattctcta tatgacttgt ggacagattt ttcacttcga catttaccaa
     5161 agtctaaggg tcgaaatcgt ttgttagagt tggcctgtgg aaccggtatt cagtcagttc
     5221 gttttgctca agcaggtttt gatgtgactg gtttagactt aagtcaagac atgctggcta
     5281 ttgctaaaaa gcgtgcgcaa tctgctaaaa agaaaattga ttttattcaa ggtaacatgc
     5341 tggatttgtc acaggtgggg cagtttgatt ttgtaacctg ctattcggat tccatttgct
     5401 atatgcaaga cgaagttgat gttggcgatg tttttaaaga agtttatgat gttttagcaa
     5461 acgatggtat ctttattttt gatgtgcatt caacctatca aacggatgag tgtttccctg
     5521 gttattccta tcatgagaat gcagatgatt ttgcaatggt ttgggatacc tatgctgacg
     5581 aggttcctca ctctgttgtt cacgaactca ctttctttat ccaagaagac gatggacgtt
     5641 tttctcgctt tgatgaagtg catgaggaac gaacgtatga gttattgact tatgatattt
     5701 tgttggaaca agcagggttc aagtcattta agctctatgc ggattttgag gataaggagc
     5761 cgactgagac tagcaagcgt tggttttttg tggcatataa atagaagaaa aaagtagcct
     5821 ttttagctat aggatttaat gaattatctt gaatgttact tatatgaaaa gagttcatta
     5881 ggcattaaat gctattatca aagcttagaa gtattggaga cattacgata aagaaagcat
     5941 tttctattat acgttgtgct taagcatttg atttaactct tattagccct ataaaagatt
     6001 atttagtaca atttgaggag attctatgac tgttaccgga attatagctg agtttaatcc
     6061 gtttcacaat ggacacaaat atttgctaga aacagcagag ggactaaaaa tcattgctat
     6121 gtcaggaaat tttatgcaaa gaggagagcc tgctcttatt gataaatgga ttcgatcaga
     6181 gatggctctt aaaaatggtg cggatattgt tgtggaattg ccctttttcg tttcagttca
     6241 atcagcagac tattttgctc aaggagctat tgatattctt tgtcaattag gaattcagca
     6301 attagcattt gggacagaga atgtgattga ttatcaaaaa ctgattaagg tctatgagaa
     6361 aaaatcagag caaatgactg cctatttatc gacgcttgaa gatacactta gctatccaca
     6421 aaaaacacaa aaaatgtggg aaatctttgc tggagttaaa ttttcagggc aaacgccaaa
     6481 tcatattttg gggttaagtt acgcaaaggc tagcgctgga aagcatatac aactatgtcc
     6541 tatcaagcgt caaggggctg cctaccattc gaaagacaaa aatcatctgc tagcttctgc
     6601 aagtgccatt agacagcacc tgaatgattg ggatttcata agtcattcag taccaaatgc
     6661 aggtttactt ataaacaatc cccatatgtc atgggaccat tatttttcat tcttaaaata
     6721 tcaaattctg aaccattctg atttaactag tatttttcag gtcaatgatg aattggcaag
     6781 tcgtattaaa aaagcgataa aagttagtca aaatattgac cacctagttg acactgttgc
     6841 taccaaacgc tatacaaaag ctcgtgtaag acgtatttta acctacattt tggttaatgc
     6901 taaggaacct actttaccta aaggaattca tattctaggg tttacctcaa aaggacaagc
     6961 acatttaaaa aaattaaaaa aaagcaggcc tctgattact agaattggag cagaaacttg
     7021 ggatgagatg acacaaaaag cagacagcat ctatcaactt gggcatcagg atatcccaga
     7081 acaaagtttt ggaagaatac ctattataat aaaaaatgag aggctcaatt aaactctaat
     7141 tgagtttttt tgggggcttg actctaatat tacgttgatg gtttatactt cagttaatcc
     7201 aatgaggaaa ttttataaca ataattaaaa aagtcagtca aaagatatgc tttcgaagaa
     7261 caggctaacg tttttacaag attatatcag taatcatttt gacaattgta gtaatggaat
     7321 gttgccaaaa tttagaatgt tgtgtgttat gaatgacaag attttaaagg aatactaatt
     7381 aaacgggagg tcgtgaaccg gaagctgttg tcactcaagc gccaaaatct gtctgtaaca
     7441 taatattgat gtatgggaac gcaattattt ggtttttatt gtgatgctta aaacgatttt
     7501 atatttctat tttaccaaag cttttcaaag tgctataata agggagttag agtcaatatt
     7561 agtaacgtta atgttgacaa tattcaataa aatactatta aaaggagaca aaaatgggac
     7621 gtaaatgggc aaatattgtt gctaaaaaaa ctgctaaaga tggagcaaca tcaaaggtct
     7681 atgccaaatt tggtgttgaa atttatgtgg ctgccaaaca aggcgagcca gatccagagt
     7741 taaatacagc tcttaaattt gtcattgatc gtgcaaagca agcacaagtg ccaaagcatg
     7801 ttattgataa agcgattgat aaagctaaag gaaacacaga tgaaactttc gtagagggac
     7861 gctatgaagg ttttggtcca aatggttcaa tgattattgt ggatactttg acatcaaatg
     7921 ttaaccgtac agcggcaaat gtacgtacgg cttacggtaa gaacggtggc aatatgggag
     7981 cttcaggatc tgtatcctac ttatttgata aaaaaggggt catcgttttt gctggtgatg
     8041 atgctgacag tgtctttgaa caattacttg aagcggatgt agacgtagat gatgttgaag
     8101 cagaagaggg aacaataaca gtttataccg ccccaacaga tcttcataaa ggtatccaag
     8161 cacttcgcga taatggtgta gaagaattcc aagttactga acttgaaatg attcctcaat
     8221 cagaagtagt attggaaggt gatgaccttg aaacttttga aaagcttatt gatgcactcg
     8281 aatcagatga tgatgttcaa aaagtgtatc ataatgtagc agatttttaa attgacatag
     8341 aataataaaa agagttgaaa actatttcag ctctttttaa gtgatgcttt tataagtaag
     8401 taccaagtaa catagtcgtt gttagaagat aatgaacaat agtaaatcag tatcaacttg
     8461 ataggaatca attattagtg aactctaatt aaaaatggta aactagacac aagaacaaaa
     8521 aggagagata ggatgattaa aaaacaatat ttaggaaggg caacaattgc cctagcatca
     8581 acccttgtac tagcagcttg tgggtcatca aaaacagctg agtcaggaaa tcaggggagt
     8641 tctaaagaag tcttatttgc aacggttgga acaacagctc ccttttctta tgaaaaaggt
     8701 ggtcaactaa ctggctacga tatcgaggtg gctaaagcag tctttaaagg atcggatgat
     8761 tacagggtgt cctttaaaaa aacagaatgg tcttctatct ttacaggttt ggactcagga
     8821 aaataccaaa tgggtggtaa caatatttcc tttaccaaag agcgttcagc taagtacctt
     8881 ttttcttatc caatcggatc aacaccttct gttttagttg ttccaaaaga cagtgatatc
     8941 aagagctttg atgatatcca agggcatacg acacaagttg ttcaagggac tacaagtgtt
     9001 gcacagttag aagactttaa taagaaacac agtgacaacc cggttactct aaaattcacc
     9061 aatgagaaca ttacccaaat gttaaccaat ttgagtgaag gcaaggcaga tttcaagatt
     9121 tttgacgctc caacggttaa cgccattatt aaaaatcaag ggttagataa tctcaaaaca
     9181 attgaactca cctcaacaga gcaaccattt atttatttta tctttagtca agatcaagag
     9241 aaattgcaaa gctttgttaa caagcgtatt aaagagttga ctgctgacgg aactcttagc
     9301 aagcttgcca aagagcacct tggcggtgat tacgtgccat cagataaaga gttgaaactt
     9361 cctacagcta actaaaccat ctattaacca gttattcatc tagaagactg gtttttctga
     9421 tcggaaacaa tattatagtt tatgaggtta tgttataaaa attgcaaata gccttaatca
     9481 aaatgagatg ttaaagtgct ttttgagaag tgaatattgt aagaagtatg ctttgggttt
     9541 tagtcctcaa ataccaagtt ttttgttgac taaacaaata aggatggtta gcatagcata
     9601 tagtttaaaa ctatgctata tgctagccat tatcaatttg aaaactatat ctaattaaga
     9661 tacaataatg atcgtaaaca aaatgaatcg aggataacgc tatgtctttt aaaaaatgga
     9721 ttggaatagt cggaatcgct ttagcatcaa ctgtattggt tgcttgttct gaaaaacaag
     9781 atgataaaaa cactctaact attggggtga tgacaaaaac agaatctgat caagcgagat
     9841 gggataaagt tgaagaatta ttgaaaaaag acaatatcac tttgaagtac aaagaattta
     9901 cagattactc gcaacctaat aaggcagttg ctaatggtga agtcgatatt aatgccttcc
     9961 aacactataa tttcttaaat aactggaata aggaaaataa ggaacatttg gtggccattg
    10021 ctgataccta tatcagccca attaaccttt tctctggaac cagtcaagat gggaaggcta
    10081 aatacaaatc agtagctgat ctgccaaatg gtgctcaaat tgcagtacca aatgatgcga
    10141 ccaatgaaag ccgtgccctc tatgtgctgc aatcagctgg cctcattaaa ttgaatgtgt
    10201 ctggtgatca attagcaacc attgccaata tctcagagaa caaaaagaaa ttagatatca
    10261 aggaattaga tgctagccaa acggcacgtg ctttagtatc tgctgatgca gctgttgtca
    10321 ataatagtta cgctgttcct gcaaaaattg actacaaaac ctctcttttc aaagaaaaag
    10381 cagatgacaa ttcaaaacaa tggattaaca ttattgcagg tcaaaaagat tgggaaaaat
    10441 cagagaaggc tgatgctatt aaaaaactca tcaaggccta ccaaactgat gaggtgaaga
    10501 aagtcgtgga aaaaacttca aatggtatag atgtatccgt atggtaaatt ttagccattg
    10561 ttataacaag agtgtggtcg tgtttaaaac acatgctttc ttttcttgca tgcttgagga
    10621 agagtttacg attaatttac ctttagaaag ctatttaaga ccttttccct tttaggattt
    10681 agaaaccttg ggaagactat tgcatgttat cttaatagtc atgattatgc aggcgatgaa
    10741 aacattgcta tcgtagatta ctgtcgccat atcgtactaa tagaggagtt attaaaatct
    10801 tatgaatgaa gcaataattc aattagatca tattgatatt accttccgtc aaaaaaaacg
    10861 ggttatcgaa gctgttaaag atgtcacggt tcatatcaat caaggagata tttatggcat
    10921 tgttgggtat tcaggtgccg ggaaatcaac tcttgtgcgt gtgattaacc tgctacaggc
    10981 accaacaaac gggaaaatca ctgttgatgg ggatgtgacc tttgaccagg ggaaagttca
    11041 gttatcagcc aatgcccttc gccaaaaacg acgtgatatt ggcatgatct tccaacattt
    11101 caatttgatg gcccaaaaaa cagccaaaga aaatgtagca tttgcgctcc gtcattcgtc
    11161 gttaagtaag actgagaaag aacacaaagt tatcgaactg ctagaacttg taggtttatc
    11221 agagcgagca gataactacc ctgcccagtt atcgggtggg caaaaacaaa gggtggctat
    11281 tgcgcgtgct ctagctaacg atccaaaaat tttgatttca gatgaggcca catcagcact
    11341 agatccaaaa accactaaac aaattttggc gctcttgcaa gaattaaacc gcaaacttgg
    11401 ccttaccatt gttatgatta ctcatgaaat gcaaattgtt aaggatattt gtaatcgtgt
    11461 tgctgttatg caaaatggtg ttttgattga agaaggttct gtccttgata tcttttcaaa
    11521 tccaaaagaa gccttgacac aggaatttat cacaacagct acagggattg atgaggcttt
    11581 agaaaaaatt aaccaacaag acattgttaa acatttgcct gcaaatgccc ttctagctca
    11641 gttgaaatat gcaggaactt ctacggatga gcctctactt aatagcattt accgtcagtt
    11701 tgaagtgaca gctaatattt tgtatggcaa tattgagatt ttagatcata ttccatttgg
    11761 tgacatgatt gttgtcttgg aagggcaagc tgaaaatatt cttgcggctg agaaagcctt
    11821 gcatgaagct ggtgttgatg ttagtatttt aaagagagga gcctagatgt cacagttaat
    11881 tcaaacttat ttgccaaatg tttatgaact cggatggtct ggagatgctg gttggggctt
    11941 agcgatttgg aatactttat acatgaccat tgtgcctttt attgttgggg gtgctattgg
    12001 tctcttatta ggcttgttat tagttttaac aggaccggac ggtgttattg aaaacaaaac
    12061 gatttgttgg gttattgata aagttacctc tgtttttaga gccattcctt ttgttatttt
    12121 gattgctatt ttagctagct ttacctattt gcttttaaga acaactttag gagcaacagc
    12181 ggccttggta cctttaacct ttgcgacttt tcctttttat gcccgtcagg tccaagttgt
    12241 tttttcagaa ttagacaagg gtgttattga agcagcacaa gcttcaggtg ctactttctg
    12301 ggatattgtc aaagtgtatc tcagtgaagg tttaccagat cttattcgtg tttctacagt
    12361 caccttaatt tctttagttg gggaaactgc catggcagga gctatcggtg ctggaggatt
    12421 aggaaatgta gcgatttctt atggttataa tcgttttaac aatgatgtga cttgggtagc
    12481 gactattatt attcttttaa ttatttttgc tatccaattt attggtgata gcttgactag
    12541 aaggtttagt cataaataaa cgaaacaaca ccctaagtaa aaacttaggg tgttgttgtt
    12601 tagatgatgt cattagcttt tcttcgtttt acttagtata agccctatta aaaaaccaat
    12661 agcagcaaat ataatccatc ccattgagaa ttgccctagt ggaacagttt tttggaaaaa
    12721 agttacaacg gcttctggta aatgaaataa tccagtcatt tgtgatagtg ttaggagtgc
    12781 atcaaatatg gcaggaataa aggttagacc aattgtagtt cgatagacaa taggatcatt
    12841 attgaagcat ttttgtgcca aaacaaggaa aattaaatcg actgttaatg ggtataaaag
    12901 gaataaaaca ggagctgacc agttgataat gactgataag ccaccaaaat aaaagaaagc
    12961 tgatagtaaa gtaaagatag ttgcccaagc aatatgagat aaagcaggca ctaatttatg
    13021 gaagtattca gcacttgacg tgattaagcc tgttgaagtg gttagacagg ccaggaaaat
    13081 cacaacagct agaaatgctt gtccgatgcc acctaggtaa aaacgagaag catgacttaa
    13141 gatttgaccg ccattaactg gattaccatg aagggtaaag ctgccatcaa taaaaggaaa
    13201 taatgattgt gatgttgctc caatacgacc gacaaagata tagacaagtg ctaatagcaa
    13261 aatggcaata gccccagaaa taagtgttat tttggtcact tctttgtccg tcttagcgcc
    13321 aaattgtttg gtagcctcaa tgactaaaat agcaaaaaca agagaagcta gtgcatccat
    13381 agtgccataa ccttgaatta atcctgctat aaaaggaaaa tctttaaagg cattattaac
    13441 accgacccca gcgttaaaag catcaccgta atttccagca ggatggacaa aggacgcgat
    13501 aaccaaaata gaaataacaa ctaacaacgt tggcgttaaa aattttccga tattttcagc
    13561 tagcttacta ggtttgatag caaggaagta ggacagacca aaaaatagta tagcataaat
    13621 agctttattg gttgtattat ctcctaagat aggagcgata ccgactgaaa aagaagtggc
    13681 tcctgttctt ggaatagcaa aaaatgggcc aatagataaa tacaaaatgg aagaatagaa
    13741 gattgcatac catttagaga tggggcgtgc caaactttca acatcgcctg atccagattt
    13801 agcaacagca atgacaccga gtaaaggcaa ggagacacca gttaaacaaa aaccaatgat
    13861 agaccataga atctgatgac ctgagtagat gcctaaaaaa gctgggtaaa tgaggtttgc
    13921 tgctccaaaa aatagcgcaa atagcatgaa tccaatgacg atatatacat ttttttgttt
    13981 catttataaa tagtctttct attgatacta atccgaagat tctaacaatt ttaattatat
    14041 tatgtgtcaa aataacaaca aaggttatta tatctaagat tacttcgata gtcaatagaa
    14101 attgactgaa aattcttagc acaaaaaatg gttaagatat gtagctcatt cgatttgacg
    14161 aatagataga attatattat tataaaaaac atgtaacttt agtaaggatg taacgtatga
    14221 aaaaaatata tgatttatgg gttagggtta gtctaatcaa aaaaataggt atcggtgttg
    14281 taataggagt catgttgggc attttggctc ctgatcttac aggttttagt attttaggaa
    14341 agttatttgt tggtggttta aaggctatcg cacctttgct tgtatttgct ttggtttccc
    14401 aagccatctc ccatcagaaa aaagggaaac agactaatat gacattaatc attgttctgt
    14461 atttatttgg tacctttgcg tcagctttag tagcagtact gacagcttat ctattccctt
    14521 tgacgttagt gttaaataca cctgtaaata cagaattatc accacctcaa ggtgttgctg
    14581 aagtttttca gtcacttcta ttaaaattag tggataatcc gatcaatgct ttagctactg
    14641 ccaattatat tggtgtgcta tcatgggcaa ttatttttgg actagcatta aaggctgcaa
    14701 gtcaagaaac aaagcatctt ataaaaacag cggcagaagt tacttcacaa atagttgttt
    14761 ggatcatcaa tctggcacct attgggatta tgagtctggt gttcactacg atttctgaaa
    14821 acggtgttgg aatcttatct gattatgctt ttttgatact agttttagta ggaacaatgg
    14881 tttttgtcgc cttagtggtt aatccactta ttgcggtttt gattacacga caaaatcctt
    14941 atccccttgt cttgagatgt ttacgtgagt caggtcttac agcatttttc acacgaagtt
    15001 ctgcagccaa tattccagtc aatatgcaat tatgccaaaa aattggattg agtaaagata
    15061 cttattcagt gtcaattcca cttggagcaa ctattaatat gggtggggca gcaattacca
    15121 tcaatgtcct aacgcttgct gctgtacaca cttttggtat tcctattgat tttctaacag
    15181 ccctcttact tagcgttgtt gctgctgtct ctgcttgtgg tgcttctggt gttgctgggg
    15241 gatcgttgtt acttattcct gtagcatgta gtttgtttgg catttcaaat gatcttgcga
    15301 tgcaggtggt tggtgttggt tttattgttg gtgttatcca ggattcttgt gagacagcct
    15361 taaattcgtc aaccgatgtt ctttttacag ctattgctga aaatgctttt tggaaacgta
    15421 aaaaagccta gctaggttta acatagtaaa aaacatcctt ggaaaacgcc aaggatgttt
    15481 ttttattatt taagatagcc aaggtaatca aatttttcaa aggtgtggtc attagcgatg
    15541 gctacaatga tagtattcgg ttctaaaggt tcaaaaggtt tgacattggt atctaaggtt
    15601 ttaacttctt tttgacgcat gccaatcaca tttaactcat atttacgacg aacatctagt
    15661 tcggaaagag attgtccttc ccagctttta ggaatgacaa attcaatcat ggaaatgcca
    15721 tgttcgaggt agatgatgct ttcgatatga cgtcttaaca gatttgaagc tacacgtttt
    15781 ccagaatcac gttcaggggt gatgacttta gttgccccga tgccatatag aacttcttca
    15841 aaaattttat ttttagcctt ggcaataatg gttggaacac cgagtttttt acagtgcatg
    15901 actgcaagaa cagaagattc caagttattc cctgaagcga taacgacagt atcacaatgt
    15961 tcaatcccta cagctaatag aaattctttg tcagtaatat ctccaacagc ggctttagtg
    16021 accaagtcgg ccacctcttt aacatgactt tctctgatat caatagcaat aacgtcttga
    16081 tcaaaattac ttaattctct ggcaacagta cggccaaaaa taccaaggcc aagaacgccg
    16141 acagttttac gttttaacat gttttctcct taacccacta aaatatcagt agtggcatat
    16201 tgaatggttt tttcttttcg ttgaatcaga ctgatgagga cagtaattgg gccaacgcgg
    16261 ccgacgaaca tcaagacaat gacaataaga cgtccagcag tagacaattg tggtgtcaaa
    16321 tccatagata ccccaactgt cgcgatggca gaaatagatt caaataagag tggtatagga
    16381 gcgatatgag gctccacgct aagtaataaa ataaaaccta tcataagaac tgcaaaaaag
    16441 aagataagaa ctgtcatggt ctgcttaatt gtcttatttg cgataattcg gttacgaaag
    16501 gtaacttctg attgccctga aagttccgct ttaaagagta agaaagtaat ggcagcagtc
    16561 gtcactttaa taccaccagc tgttccacca ggagcaccac caataaccat ttgaatcata
    16621 taaagaatgt tagtcggtgc tagggtatcg ttataagaga ttgttgcaaa gccagctgtt
    16681 ctcatagtga ctgtttgaaa aaatgaaacc ataagttgct gatgtaaact aaaattggca
    16741 atggttttgc tgttatcttt ttctaaaaac caggttaaga aggttccaag aaaaagaata
    16801 acagctgtgg tttgtagtac tagacgagac tgattggata atttacgaaa agttgcccca
    16861 taacaatgag gtctttcaaa aaagtatttt ttaaaagcta ctcctaaatc aacccaaacc
    16921 gcaaaaccaa gtccaccaga gataatcaag aacgtaatga taacatttaa tgtaggattt
    16981 aacataaagt cttttaaact agaagatcct aaattgtcaa aaccagcgtt acaaaatgcc
    17041 gacacagcta gaaaaatact attgaagata ccatttttcc acccgaagcg ggggataaag
    17101 tcaatcatga taactatcgc agcaaaggct tctatactaa aggttacttt ataagcaaaa
    17161 aaaaggtagt gctttaaatc tttgctatca cctcgattta acgctgattg gagcaaggtt
    17221 tgatcgctca atcgcatttt tcgcttgagg gcaaaagtac tcacagctat taacgtcacc
    17281 aagccaagac caccaatctg cattagtgcc atagcaatgg tttgtccgat accgttatat
    17341 acttcagcga cgggaacaac agaaagtcca gtcacacaaa ccatggaaac cacattaaag
    17401 aaatgatcaa gatagacggt attaggacca ttttgataat gggtaaaggg catggataac
    17461 aagagagtcc caatcagaat cacaatggca aaactaaatg ttaagcgctg tgtgactgaa
    17521 agagatttga taaaagagcg tttcatgttt cctccagaca taatatctcc tactataata
    17581 acaaaaataa gttaaaaaag ctaagtttca tcttagcctt tggtactatt ttatagaggt
    17641 tttttgttag gcaagccagc ttttcttgga tacttgttag gtgtttcctt tttcttctca
    17701 acaatggtga tgaagcgaga atctccattt ggcaattggt agctatggtt tttaatgact
    17761 ttaccaaaaa gaagacaaag agcattctta gcttcttcta actcttggtc agcagcttgt
    17821 gcctttaagg cgattaattt tcctccaatt tttaaaaagg ggatggttaa ttcagataga
    17881 acctgcatcc gagcaaccgc tctggcagta acgacatcaa attgacctcg aaaggccttg
    17941 tcctgtccga aatcttctgc tctaccatga aaaaaatgga cattttccaa gcccaattcc
    18001 tgagcaagga gtgtcagaaa agaaatccgc ttattaagag aatcaataat ggtaacctca
    18061 aggttcggga aaagaatttt catgggtagg ctaggaaaac cagctccagc tccaatgtcc
    18121 aataatttga ttggttcatt agctaaaaag gcctgtaaaa taggagcgat ggaatcataa
    18181 aaatgcttga ggtaaacctc attttcttct gtaatagctg ttaaattaat tttagtgttc
    18241 cattccacta gtaatttaaa gtaggtgtca aattgttctt tttgtttcga tgataaggaa
    18301 aaaccatctt cctctagtgt tcggtaaaag tcttggggtg tcatttgtat cctctgtttt
    18361 tgatagttgt taccatttta tcataaaata gcccataaca aaatccaata aatcatcaga
    18421 aataacacct cattacttgc tattagccta gaaattgcta taattgagac acaaagatta
    18481 aaggagaccc actatgccaa cagttttgat tatcttagtc gttttaggag tgcttgcgct
    18541 atggttaatg attagttaca atagcctggt taaatctcgg atgcacacaa aagaagcttg
    18601 gagtcagatt gatgtgcaat taaagcgtcg taatgacttg attccaaatc ttattgaaac
    18661 cgtaaaggga tatgctagct acgagcaaaa gacatttgaa aaaatcactg atttgcgtgc
    18721 gcgtgttgcg aatgcctcaa ctcctcaaga aaccatggcg gcttctaacg aattgagtaa
    18781 acaagtgacc agtttgtttg ccgttgctga aaattaccca gacttaaaag ctaacgaaaa
    18841 cttcttgaaa ttacaagaag agttgaccaa tacggaaaat aaaatctcat attctcgtca
    18901 actctataat tcaacaacgt ctaattacaa ccttcaatta gaatctttcc caagcaatat
    18961 cgctggtaaa ttatttggtt ttaaaccaag tgaattctta caaacaccag aagctgaaaa
    19021 agaagttcca aaagttgaat ttaactttta aatatgaaat tatcatctag taggcaatca
    19081 taagagaaag gagaattatg ctttatcaac agatttcaca aaataagcaa cgaacagttg
    19141 ttttattagt tgttttcttc gctctcttag cacttattgg agcgtcagca ggctatctgc
    19201 tgttagataa ttatgccatg ggcttggttc ttgctcttgt cattggggtg atttatgcta
    19261 ccagtatgat ttttcaatcc accagtcttg taatgagtat gaacaatgct agagaagtta
    19321 cagaaaaaga ggctccaggc ttcttccata ttgtcgagga tatggctatg gtggctcaga
    19381 ttccgatgcc gagagttttt attattgaag atccttcttt aaatgctttt gcgacaggat
    19441 ctagccctca aaatgctgct gttgcagcaa cgacaggttt acttgaagtt atgaatcgtg
    19501 aagaacttga aggtgttatc ggccatgaaa ttagccacat ccgaaattat gatatccgta
    19561 tttcgactat tgctgtagct ttggcgagtg ctgttaccgt tatttctagt atcggtgggc
    19621 gtatgctatg gtatggtggc ggttctcgca ggcaaagaga tgacggagat gatgatgttt
    19681 taagaatcat tacgttactt ttgtcgttat tatctttgct tttagcgccc ctagtagcaa
    19741 gtttgattca gttggctatt tctcgtcaaa gagaatatct agctgatgct agttctgttg
    19801 aactaacaag aaatccccaa ggtatgatta aggctcttga aaaattgcag ttatctcagc
    19861 caatgaagca tcctgttgat gatgctagtg cggccttgta tattaatgag cctcgcaaaa
    19921 aaaggagctt cagttcatta ttcagcaccc atcctcctat tgaggagagg attgaaaggt
    19981 taaaaaacat gtgagcgttt ggggtttttg ctcaagcctc acttttttat gtctttgggc
    20041 agataatcta aaatggcagg aattatcaaa aatatctgaa caaaaagaaa aaaatgagtg
    20101 aaacaatggt taaaaataga atgatttaaa tcttaaaatt aaaaaacgtc
//
LOCUS       DAUDYX010000039        19899 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.039, whole genome shotgun sequence.
ACCESSION   DAUDYX010000039 DAUDYX010000000
VERSION     DAUDYX010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19899
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.039"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            80..1912
                     /gene="lepA"
                     /locus_tag="VHD02_001366"
     CDS             80..1912
                     /gene="lepA"
                     /locus_tag="VHD02_001366"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEQ5497970.1"
                     /translation="MNSQDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRHEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGIVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYVAASIKTVAD
                     TRVGDTVTLANNPAKEALHGYKQMNPMVFAGIYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVHTTDEDMIE
                     VSNPSEFPDPTRVAFIEEPYVKAQIMVPQEFVGAVMELSQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYDMSEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKIIVEKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDAKK"
     gene            2150..3118
                     /locus_tag="VHD02_001367"
                     /pseudo
     CDS             2150..3118
                     /locus_tag="VHD02_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164232585.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            3304..3741
                     /gene="msrB"
                     /locus_tag="VHD02_001368"
     CDS             3304..3741
                     /gene="msrB"
                     /locus_tag="VHD02_001368"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEQ5497971.1"
                     /translation="METSDELKQRIGDLSYEVTQHAATESPFTGEYDDFFEKGIYVDI
                     VSGEVLFSSLDKFNSGCGWPAFSKPIENRMVTNHDDSSYGMRRVEVKSREAGSHLGHV
                     FSDGPKEAGGLRYCINSAALKFIPYDQMEKEGYAQWLTLFDET"
     gene            3871..4890
                     /locus_tag="VHD02_001369"
     CDS             3871..4890
                     /locus_tag="VHD02_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEQ5497972.1"
                     /translation="MSTHLRKLPGLLLCLLLALPAWCLGRLFPIIGAPVFAILLGMLL
                     ALFYEHRDKTKEGISFTSKYILQTAVVLLGFGLNLTQVMAVGMQSLPIIISTIATALL
                     VAYGSQKWLRIDVNTATLVGVGSSICGGSAIAATAPVIKAKDDEVAKAISVIFLFNML
                     AALLFPSLGQLLGLSNEGFAIFAGTAVNDTSSVTATATAWDALHHSNTLDGATIVKLT
                     RTLAILPITLGLSLYRAKKEHDIVTEEGFSLRKSFPRFILFFLLASLITTLMTSLGVS
                     ADVFHSLKTLSKFFIVMAMAAIGLNTNLVKLIKTGGQAILLGAICWVAITLVSLAMQL
                     SLGIW"
     gene            5097..5522
                     /locus_tag="VHD02_001370"
     CDS             5097..5522
                     /locus_tag="VHD02_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ5497973.1"
                     /translation="MKRKFLIGSHGRLASGLQSSIDILAGMGQALETIDAYVDDSDYT
                     SQIDDFIAGVAADEQGLIFTDLLGGSVNQKMVTAVMNSGKDNIFLITNSNLATLLSLV
                     FLKPGEALTKDEIVTVINESQVQLVDLVPETNSEDDFFD"
     gene            5541..6032
                     /locus_tag="VHD02_001371"
     CDS             5541..6032
                     /locus_tag="VHD02_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEQ5497974.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEDSIKLFNDPRATDKRIFVIVNSVKDACAIAKEVPDLEAVN
                     VANVGRFDKSDPASKVKVTPSLLLNPEEMAAAKELVSLPELDVFNQVLPSNTKVHLSQ
                     LVN"
     gene            6049..6858
                     /locus_tag="VHD02_001372"
     CDS             6049..6858
                     /locus_tag="VHD02_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEQ5497975.1"
                     /translation="MLVPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGLLLGDM
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISHIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNAFVEHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDLELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            6855..7682
                     /locus_tag="VHD02_001373"
     CDS             6855..7682
                     /locus_tag="VHD02_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEQ5497976.1"
                     /translation="MTSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASAGSAMGAVVFLLLYNIPAFIIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQDYLDQLFIGIVPLMVTLAAFWLLRKKVNINWIMFGIMFLGIILGLLGIC"
     gene            7818..9467
                     /locus_tag="VHD02_001374"
     CDS             7818..9467
                     /locus_tag="VHD02_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ5497977.1"
                     /translation="MRGEQVEEHFKKQLQDDISRHFSYQSLMLSLLLIGLFIIFSLAP
                     QQLGLYRDINATATRYHRLISKQEALLDDLGKNSLLPFLNKNLSTADLSKHYFHLRHS
                     SQTSPELLLFSPNQGLLFASNPHLGNVFSKSVYIQEVLRAAHPPKTLFKVAMDSEDGH
                     YLMIIKPMIDQNQLKGYAFLVMSGKDFLHPAKTLTSELVIADKLDNTFTFSNREFIAS
                     SLDKINSQYLHHYFVFQDNRAFITRKVALQGGLWLYMYRPLIPMVSVMLFSLISSAVI
                     FVILQRKSSGLANRIAAKNSRAINQMVRDMSAISRQEKRRIDLESQDEFQYLSDQINQ
                     MVERLQQLHDKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSHYDSALTEKIV
                     IQLTKLLRYSLTDSSKPVLLKDDLSVIESYLVINQVRFEELQYSINLSPDLDSLAVPK
                     LFLLPLIENAIKYGLKERHDVKINIACYYQDDHIIFSVRDNGSGIDAHHQKVIREQLE
                     AGESHHGLINSYRRLKYHFSEVSLVFDQGDKQFNVSYHVKE"
     gene            9471..10259
                     /locus_tag="VHD02_001375"
     CDS             9471..10259
                     /locus_tag="VHD02_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ5497978.1"
                     /translation="MYKLVIIEDEHIIRKWLRYAIDYKALDILVIGEAKDGKEGAVLI
                     KESQPDIVLTDINMPIMTAFDMFEATKDQTYAKIILSGYADFPNARSAIHYGVLEFLT
                     KPIEKAALWECLQTIIAKIEKQKGSNQKTDACVYIPLPQMTDQIPEVVKDILEWVHAH
                     FQDKISTSRLAHDLGYSESYIYQNIKKHLQMPLSDYINQYRINQAIQLMQQEPDLMVY
                     EIAQAVGIYDYRYFDRVFKKYLGQTVKAFKEEHFMKDTDRSKPC"
     gene            10253..11299
                     /locus_tag="VHD02_001376"
     CDS             10253..11299
                     /locus_tag="VHD02_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ5497979.1"
                     /translation="MLKLKRKWLLSFLLVIIILAIVNVAMCIFSSSKKDSAKELVILT
                     PNSQTILTGTIPAFEEKYGVKVRLIQGGTGQLIDQLGRKDKPLNADIFFGGNYTQFES
                     HKDLFESYVSPQVSTVISDYQLPSHRATPYTINGSVLIVNNELARGLHITSYEDLLQP
                     ALKGKIAFADPNSSSSAFSQLTNILLAKGGYTSSEAWEYVKRLLLSMNSIRATSSSDV
                     YESVAEGKMIVGLTYEDPCINLQKSGANVSIVYPDEGTVFVPSSVAIIKKAKAMKEAK
                     QFINFILSSDVQNAFGQSTSNRPIRRDAQTSHEMKPLDEIITLKEDYGYVTRHKKKIL
                     NTYNRLHEALERGK"
     gene            11414..12811
                     /locus_tag="VHD02_001377"
     CDS             11414..12811
                     /locus_tag="VHD02_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEQ5497980.1"
                     /translation="MTYQTIYPYTNEVLHTFDNMTDQGVADVLERAHLLYKKWRKEDH
                     LEERKAQLHQVANILRRDRDKYAEIMTKDMGKLFTEAQGEVDLCADIADYYADKADEF
                     LMSTPLETDSGQAYYLKQSTGVILAVEPWNFPYYQIMRVFAPNFIVGNPMVLKHASIC
                     PRSAQSFEELVLEAGAEAGSITNLFISYDQVSQVIADKRVVGVCLTGSERGGASIAEE
                     AGKNLKKTTLELGGDDAFIIFDDADWDQLEKVLYFSRLYNAGQVCTSSKRFIVLDKDY
                     DRFKELLTKVFKTAKWGDPMDPETTLAPLSSAQAKADVLDQIKLALDHGAELVYGGEA
                     IDHPGHFVMPTIIAGLTKDNPIYYQEIFGPVGEIYKVSSEEEAIEVANDSNYGLGGTI
                     FSSNQEHAKAVAAKIETGMSFINSGWTSLPELPFGGIKHSGYGRELSELGFTSFVNEH
                     LIYIPNKTNNSNTKV"
     gene            12913..14709
                     /gene="uvrC"
                     /locus_tag="VHD02_001378"
     CDS             12913..14709
                     /gene="uvrC"
                     /locus_tag="VHD02_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEQ5497981.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKEGTIIYVGKAKNLKKRVRSY
                     FRGSHDTKTELLVSEIVDFEYIVTESDTEALLLEINLIQKNMPKYNIKLKDDKSYPFL
                     KITNESFPRLVITRYIKKNDGLYFGPYPDSYTANEVKKLLDRIFPFKKCKNPINKVCF
                     YYHLGQCCAHTICHTDKAYWDRLIDDVKHFLNGKDDKIIEDLRSKMLAASEEMAFERA
                     AEYRDLISGIATMRTKQRVMSKDLQDRDIFGYYVDKGWMCVQVFFVRQGKLIQRDVNL
                     FPYYNDAEEDFLTYMGQFYQDKQHFIPKEVFIPEAIDEELVAAIVPTKIIKPKRGEKK
                     QLVALATKNARVSLQQKFDLLEKDIKKTSGAIENLGQLLRIDKPVRIEAFDNSNIQGT
                     SPVAAMVVFVDGKPSKKDYRKFKIKTVVGPDDYASMREVLFRRYSRVKKEGLQAPNLI
                     IVDGGVGQVNVAKDVIEKQLGLTIPVAGLQKNDKHQTHDLLFGNPLEVVPLPRRSEEF
                     FLLHRIQDEVHRFAVTFHRQVRRKNSFSSTLDHISGLGPKRKQLLLRHFKTITAIASA
                     TSEEIQALGIPKTVVEAIQQQITDNKNDRSSP"
     gene            14893..15495
                     /locus_tag="VHD02_001379"
     CDS             14893..15495
                     /locus_tag="VHD02_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEQ5497982.1"
                     /translation="MKFLELNKKRHAIKTFNDQPVDYEDLRTAIEIATLAPSANNIQP
                     WKFVVVQEKKAELAKGLPLANKVQVEQAQYVVALFSDTDLALRSRKIARIGVKSLPDD
                     LIGYYMETLPPRFAAFNEVQTGEYLAINAGIVAMNLVLSLTDQKIASNIILGFDKSTT
                     NEILDIDPRFRPELLITVGYSDEKPEPSYRLPVDEVIERR"
     gene            15620..17029
                     /gene="pepV"
                     /locus_tag="VHD02_001380"
     CDS             15620..17029
                     /gene="pepV"
                     /locus_tag="VHD02_001380"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEQ5497983.1"
                     /translation="MTTIDFKAEVDKRKEAMLADLVDLLRINSERDDTLADDKHPFGP
                     GPVKALEHFLAMAERDGYKTRNIDNYAGDFEFGQGDEVLGIFGHLDVVPAGSGWDTDP
                     YEPVIKDDRIYARGSSDDKGPTMACYYALKIIKELGLPVSKKVRFIVGTDEESGWGDM
                     DYYFAHNGLKNPDFGFSPDAEFPIINGEKGNITEYLHFAGDNNGAFVLHRFQGGLREN
                     MVPESATAVITSPHDLDVLEAALEQFLSEHGVKGSMKATDGKIEVTIIGKSAHGSTPE
                     AGVNGATLLAKFLNQFTFEGAAKDYLYVAGEVLHEDFAAEKLGLAYTDDRMGALSMNA
                     GVFTFDSQSADNTIALNFRYPKGTDAATLKAGLEKLPGVIKVSLSEHEHTPHYVPMDD
                     ELVATLLAVYEKQTGLKGYEQVIGGGTFGRLLERGVAFGAMFPGDENTMHQANEYMPL
                     ENIYRSAAIYAEAIYELIK"
     gene            complement(17097..18473)
                     /gene="mnmE"
                     /locus_tag="VHD02_001381"
     CDS             complement(17097..18473)
                     /gene="mnmE"
                     /locus_tag="VHD02_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002888396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEQ5497984.1"
                     /translation="MSITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKN
                     LEQVASHTINYGHIIDPKTGTIIDEVMVSVMLAPKTFTRENVVEINTHGGIAVTNEIL
                     QLLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLS
                     QLINDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLREKTQEFQSLLENLLRTAKRG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTDQDRALLNLSQDSNRI
                     ILLNKTDLEQKIELEQLPADLIPISVLTNQNINLIEDRINQLFFDNAGLVEQDATYLS
                     NARHISLIEKAVQSLEAVNDGLALGMPVDLLQVDLTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
     misc_feature    18645..18780
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            18809..19309
                     /locus_tag="VHD02_001382"
     CDS             18809..19309
                     /locus_tag="VHD02_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEQ5497985.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVDQDTVLRR
                     SLRESGVEFKVIKNSILTRAAEKAGLDELKDVFVGPSAVAFSNEDVIAPAKVINDFTK
                     TADALEIKGGAIEGAVSSKEEIQALATLPNREGMLSMLLSVLQAPVRNVAYAVKAVAE
                     NKEGAA"
     gene            19374..19739
                     /gene="rplL"
                     /locus_tag="VHD02_001383"
     CDS             19374..19739
                     /gene="rplL"
                     /locus_tag="VHD02_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEQ5497986.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGAEEAAKDSFDVELTSAGDKKVGVIKAVREITGLGLKEAKGLVDGAPANVKEGVAAA
                     EAEEIKSKLEEAGATITLK"
BASE COUNT         6133 a         3649 c         4218 g         5899 t
ORIGIN      
        1 taatccgtaa gaaaagatag gcaaaggcct accttttttg ttataataga aggaaagact
       61 aaatgtatag gaaaatagaa tgaacagtca agatttaaaa aaacgtcagg agaagattcg
      121 taatttctcc attattgcac atattgacca tggcaaatct actctcgctg accgcatttt
      181 ggaaaagaca gagacggttt cgtctcgtga aatgcaggct cagttattgg actccatgga
      241 tttggagcgt gagcgtggga ttaccatcaa gttaaacgcc attgagctta attacacggc
      301 taaagatggg gaaacctaca ttttccacct tattgatacc ccagggcatg tggactttac
      361 ttatgaagta tcgcgttcgt tggcagcctg tgagggagcg attctagttg tggatgcagc
      421 gcagggtatt gaagcgcaga cccttgctaa tgtttaccta gcccttgata atgatttgga
      481 aattttaccg gttatcaata aaattgattt gcctgctgct gaccctgaga gagtccgcca
      541 tgaagtagaa gatgtcattg gacttgatgc ttctgaggct gttctggcgt cagccaaggc
      601 tggtatcggg atcgaagaga ttcttgagca aattgttgag aaggttcctg ctcctacggg
      661 tgatgtggat gcgcctttac aggccttgat ttttgactct gtttacgatg cttatcgagg
      721 cgttatcctg caggttcgga ttgtgaacgg tattgtcaag cctggcgata aaatccagat
      781 gatgtcgaat ggaaaaacct ttgatgtcac agaagttggg attttcactc ctaaggcagt
      841 tggacgagat ttccttgcaa cgggagatgt tggttatgtt gcggcttcta tcaaaacggt
      901 agcagatacc cgtgtggggg atacggtgac tttagctaat aaccctgcta aagaagcctt
      961 gcatggctac aagcagatga acccgatggt ctttgcaggg atttatccga ttgaatcaaa
     1021 taaatacaat gacttgcgtg aagcgcttga aaaattacag cttaacgatg ccagtttaca
     1081 gtttgaaccc gaaacgtcac aagcgcttgg ctttggtttt cgatgcggct ttttaggctt
     1141 gcttcacatg gatgtgattc aagaacgttt ggagcgtgag tttaacattg atttgatcat
     1201 gacagcacca tctgtagtct accatgttca cacaacagat gaggacatga ttgaagtgtc
     1261 taacccttca gaatttccag acccaacccg ggtggctttt attgaagagc cttatgttaa
     1321 agcgcaaatc atggtgccac aagagttcgt aggggctgtt atggaattat cgcagcgcaa
     1381 gcgtggtgat tttgtgacca tggattacat tgatgataac cgtgtcaatg tgatttacca
     1441 aatcccgctg gctgaaattg tctttgattt ctttgataaa ttgaaatcct caacgcgagg
     1501 ctatgcgagt tttgattatg acatgtcaga ataccgcagg tcacaattgg tgaaaatgga
     1561 cattcttttg aacggcgata aggtcgatgc gcttagcttc attgttcata aagaatttgc
     1621 ttatgaacgc gggaaaatta ttgttgagaa attgaaaaaa atcattccac gtcaacaatt
     1681 tgaagtgccg atccaagccg ctattggtca aaaaattgtt gcgcgttcag atatcaaggc
     1741 tcttcgtaaa aacgttttag cgaagtgtta tggtggtgac gtgtctcgta aacgtaaatt
     1801 gctcgaaaaa caaaaggctg gtaaaaaacg gatgaaggct attggttctg tcgaagtccc
     1861 tcaagaagcc ttcttgagtg ttctttcaat ggatgacgat gccaagaaat aaccaagcct
     1921 aatcgcttag tcttcagagg agaactcttg tgggcctaca tgaccaaaaa attgattatc
     1981 tcataaaaaa agaactttac aatctctttc acataacact ataatggtat tatagtgtta
     2041 tctcattatt aggtgagcct gcatttaatc ttattaaaga gaaagaggaa acaaaaactg
     2101 tgaacaaaac aaaacaaaac aaaacaaaac aaaacaaaac aaaacaaaac aaaacaaaac
     2161 aaaacatagc cttctttgtc gctatggctt aacgtcagcg gctgctcttt tgctggcctt
     2221 tggaggtgca agtgcggtta aggcggatca acagccaact attgaagaga cacagagtca
     2281 attacaggac ttacgcgaag ctttccctaa actagaagat agttatcaag agtgggatat
     2341 ctttaggcaa aacaaaattt ataagcattt agatgagttt gagcataagc taaatagcta
     2401 tttgaaagta ttagatggaa gtttatctca gttaaagggt gaaaaaggtg aacgtggtcc
     2461 agtaggtccc gctggtccac aaggccttca aggtaccaaa ggtgaccgcg gtgaaactgg
     2521 tgaacaaggt caacgaggcg aaaccggtcc cgctggccca caaggtcccg caggtccagt
     2581 aggtccagct ggaaaagatg gtgaagctgg agctcaaggt ccagtaggtc cagctggcaa
     2641 ggacggccaa gatggtaaag atggtcttcc aggcaaggac ggccaaaacg gtaaagatgg
     2701 tctcccaggt aaagacggta aggacggcca agatggcaaa gatggtcttc caggtaaaga
     2761 cggtaaagat ggcctcccag gtaaagacgg taaggacggt caaccaggta aaccggctcc
     2821 taaaacacca gaggtccctc aaaaaccaga taccgcacca catacaccaa aaacccctca
     2881 gatccctggc caatcaaaag acgtgacacc tgcccctcaa tccccttcta atagaggtct
     2941 aaacaaacca caaacacaaa gtggtaatca gctcgcaaaa acaccagcag ctcacgacac
     3001 acacagacaa ttgccagcaa caggcgaaac aaccaatcca ttctttacag cagctgctgt
     3061 agctatcatg acgacagctg gagttgtagc tgttgcaaaa cgtcaagaaa acaactaatc
     3121 ctctttttgc tacctaactg atggaaagta aaactcagag agaacctcaa ggttctctct
     3181 tttttgaatc aagatagtag ctctaggtat aacgtcttgt ctgaggtgct gtgaacaaat
     3241 cagttactgc tcacctcttt ttttggtaaa atgtaagcac taaaacatca aaaggagttt
     3301 tccatggaaa catcagatga gttaaaacaa cggattggtg atctttctta cgaggtcacc
     3361 cagcatgcag caacagaatc tccctttaca ggggaatatg atgatttttt tgagaagggt
     3421 atttatgtcg atatcgtcag tggtgaggtc ttgttttcat cactggataa atttaattct
     3481 ggctgtggtt ggcctgcctt ttcaaaacct attgaaaatc gtatggtcac aaaccacgat
     3541 gactcttctt atgggatgag acgagttgag gttaaaagcc gtgaagcagg atcgcatttg
     3601 gggcatgtct ttagtgacgg ccccaaagaa gctggaggct tgcgttactg tatcaattca
     3661 gcagctctta aattcattcc ttatgaccag atggaaaaag aaggctatgc ccaatggctc
     3721 acattgtttg atgagacata acagggtctc aaaatgacta aaaaagaagc gtcagtgatt
     3781 cccataagca ctggattggt gaggcgcacg ggtcaatttt acaagccttt gtaaaaggtc
     3841 tataataggg aatgaaaaaa ggaggttagt atgtcaactc acttaagaaa acttccaggc
     3901 ctgttacttt gcttattgtt agctcttcca gcctggtgtt tagggcgctt gtttcctatt
     3961 attggagcac ctgtttttgc tattctttta ggaatgttgt tagccttgtt ttatgaacat
     4021 cgtgacaaga ctaaagaggg aattagtttt acatccaagt atattttaca aacagcagtg
     4081 gttttgcttg gttttggatt aaacctaacc caagttatgg cagtgggcat gcagtcttta
     4141 ccgattatca tttcaactat tgcgacagct cttttggtag cttatggctc acagaaatgg
     4201 ctgcgcatag atgtcaatac agccaccttg gtgggtgtag ggtcttccat ttgtgggggg
     4261 tctgctattg cagcgacagc tcctgtcatt aaggcaaagg atgacgaggt tgctaaggca
     4321 atttcagtca tttttctctt taatatgtta gcagctttgc tatttccaag tctaggacaa
     4381 ttactgggct tatctaatga aggttttgct atttttgctg ggacagctgt taacgacacc
     4441 tcttcagtaa ccgcgacagc aacagcctgg gatgcccttc accattccaa tacactagat
     4501 ggcgcaacca ttgtcaaatt gactcgcacc ttggctattc ttccaattac tttaggctta
     4561 tccctttacc gagcgaaaaa agagcacgac atcgttacag aagaaggctt cagccttagg
     4621 aagtcattcc ctcgcttcat cctcttcttt ttattagctt ccctcatcac gacattgatg
     4681 accagtttgg gtgtttctgc cgatgttttc cattccctaa aaaccttatc aaaattcttt
     4741 atcgtgatgg ctatggcggc tatcggttta aacacaaact tggttaaact gattaagacg
     4801 ggcggtcagg ctatcctttt aggggctatt tgctgggtag ctatcaccct tgtcagttta
     4861 gccatgcaat taagtttggg catttggtaa tcagacacga gaaatcagtt tgactgattt
     4921 ctttttttgt atcttatttg tgtagaattg tctacatttt aaagagaata atgcatttta
     4981 aaaacgtttt catttggtaa aataaaccta tcgttaaacc agttcagtaa agacaactgg
     5041 ttgagataag caattaccgt ttattcagcg gtttttgtta atagaggagg ataggaatga
     5101 aacgaaaatt tctcatcggg agtcacggca gactagccag tggtttgcaa agctctattg
     5161 acattttagc aggtatggga caggcacttg aaaccattga tgcttatgtt gatgatagtg
     5221 actacactag ccagattgat gactttatcg ctggggtagc agcagatgag cagggcctta
     5281 tttttactga tttgttaggc ggcagtgtga atcagaaaat ggtaaccgct gtaatgaaca
     5341 gtggcaaaga caatatcttt ttaatcacaa acagtaactt agccacttta ttgtcgttgg
     5401 tgtttttaaa accaggcgaa gcgcttacta aagatgaaat tgtaaccgtt atcaatgaat
     5461 cgcaagttca gttagtggat ttggtccctg agacaaattc agaagatgac ttttttgatt
     5521 aatatctagg aggaagacaa atgattactc aaattcgtgt tgacgaccgt ttgattcacg
     5581 ggcaagtggc agttgtatgg accaaagagt taaatgcgcc gcttttagta gtggctaatg
     5641 atgaagctgc aaaaaatgaa atcacacaaa tgacgcttaa aatggctgtg ccaaacggca
     5701 tgaaactgct tattcgttca gttgaagact cgattaagct ctttaatgat ccacgcgcaa
     5761 ctgacaagcg tatttttgtg attgtcaatt ctgttaaaga tgcttgtgcc attgctaaag
     5821 aagttccaga cttagaagct gttaatgtgg ctaatgtggg gcgttttgac aaatcagacc
     5881 cagcaagcaa agtcaaagtg acaccaagtc ttttacttaa cccagaagaa atggcagccg
     5941 caaaggaatt agtcagttta ccagaacttg atgtctttaa ccaagtctta ccatcaaata
     6001 ccaaggtcca tttgagccaa ttagtcaact aaagagaggt aaaaaagtat gttagtacca
     6061 gcaacaatgg cagctttagc tgtattgatt tgttttgggg gaaattattt aaccggtcaa
     6121 agtatgatgg aaagacccct agtagttgga ctggtcacag gtcttctttt aggagatatg
     6181 aaggtcggta tcttaatggg ggcctctcta gaagctcttt tcctaggaaa tgtgaatatt
     6241 gggggtgtta ttgcagctga gcctgtaaca gcgactgcca tggcaacaac ctttaccatt
     6301 atttcacata ttgaccaaaa agcagccatg accctagctg ttccaattgg aatgttggca
     6361 gcctttgtgg ttatgttttt gaaaaacgtc ttcatgaata tctttgctcc aatggttgat
     6421 aaagctgcag cagctaacca tcaaggtaaa ctagtgatgc tccactatgg cacttggatt
     6481 atctattact taatcattgc ctctatttct ttcattggta ttttagtagg tagtggacct
     6541 gttaatgcct ttgtggaaca cattcctcaa aatctcatga atggcttgag tgctgcggga
     6601 ggccttcttc cagctgtcgg ttttgccatg ctgatgaaat tattgtggac caacaaatta
     6661 gctgtttttt acctattggg ttttgtctta acagcttatt tgaaattacc agcggttgca
     6721 gttgctgccc taggagcagt tatttgcgtt attagttccc aacgtgatct agaattagac
     6781 gcaattacta gaggtgcaat tagtaaacaa acaacctttg actctaaaga atcagaagag
     6841 gaggacttct tcgcatgaca tcacaagata atttgactaa agaagaccgc aaaatgcttc
     6901 gctcagtctt ttggcgctca tggacgatga atgctagtcg tactggggca acacaatacc
     6961 acgcagtagg ggttatttac actttactac cagtcatcaa tcgtttttat aagactgata
     7021 aggataaagc agaggcactg gtccgtcata caacatggtt caatgccacc atgcacatca
     7081 ataactttat catgggtctt gtggcatcga tggaaaagaa aaacagtgaa gatcctgatt
     7141 ttgatgctag tgctatcacc gctgttaagg cttcgttgat gggacctata tctggagttg
     7201 gagattcctt cttctgggga attttaaggg ttattgcggc aggtatcggg atttccttgg
     7261 caagtgcagg ctcagccatg ggagctgttg tcttcttact cttgtacaat attcctgctt
     7321 ttattattca ctactatagc ctttatggtg gttattctgt aggtgcagga tttatcaaaa
     7381 aactttacga atctggtggt ataaaaatcg tcactaaaac ctcaagcatg cttgggttaa
     7441 tgatggtagg ctcaatgacg gcttcaaatg taaaatttaa aacgattctt acagttgctg
     7501 cgaaaggtgc caaagaagca gcttctattc aagattacct tgaccaattg tttattggta
     7561 ttgtaccatt aatggtaaca ctagcagctt tttggcttct acgaaagaaa gtgaatatca
     7621 actggattat gtttggtatc atgttccttg gcattatcct tggactttta ggtatttgct
     7681 aataatctcc tagcaatgaa ggtaagtaag acaagttttc ttgttttgct tacctttttc
     7741 cttttaagat gatgagactt gttttgatga aaaacgtagt agcattaaga aatttgttat
     7801 aatgaaagga gcttaagatg cgaggtgaac aggtggaaga acattttaaa aaacaattac
     7861 aagatgatat ttctagacat ttttcgtacc aatcgttaat gttatcattg ttgctaattg
     7921 gtctttttat tattttttca ttagcgccac aacaattagg cctctatcgt gatatcaatg
     7981 ccactgcaac ccgttatcac cgtttgatta gcaaacaaga agccttgctg gatgacttag
     8041 ggaaaaatag cttactacct tttttaaata aaaacctcag cactgctgat ttaagcaagc
     8101 actattttca tttgcgccat agcagccaaa cgtcaccaga acttttacta ttttcaccta
     8161 atcaaggcct tttatttgct agtaacccac atttaggaaa tgtttttagt aaatctgttt
     8221 atattcaaga agtcttgaga gcagctcatc ctccaaaaac cttgtttaaa gttgctatgg
     8281 atagtgaaga tggtcactac ttgatgatca ttaagccaat gatagatcaa aaccaactaa
     8341 agggatatgc ttttttagtg atgagtggta aagattttct tcatcctgca aaaacattga
     8401 cgtcagagtt ggtcattgct gataagctgg acaatacgtt tacgttttcc aatcgtgagt
     8461 ttatagcatc tagtctagat aagatcaaca gtcagtattt acaccattat tttgtttttc
     8521 aagataatcg agcctttatc acaagaaagg ttgccttaca aggaggtctt tggctttaca
     8581 tgtataggcc cttgattcca atggtatcgg tgatgttatt ttcacttatt tcatcagctg
     8641 ttatctttgt gattttgcaa cgtaaatcaa gtggtttagc taaccgaatt gcagctaaaa
     8701 attcaagagc aatcaatcaa atggttagag atatgagtgc gatctctcgg caagaaaaaa
     8761 gacgtattga ccttgagagt caagatgaat ttcaatattt atctgatcaa atcaatcaaa
     8821 tggtagagcg attgcaacag ttacatgata aaacgttaga tttggaaact caaaaattat
     8881 tatttgaaaa acggatgtta gaagctcaat tcaatccgca tttcctctac aatacgctag
     8941 aaaccatttt gattacgagc cattatgatt ctgccctaac agaaaaaatt gttatccaat
     9001 tgacaaaatt gttgcggtat agtctcacgg attctagcaa acctgtcttg ctcaaagatg
     9061 atttaagtgt catcgagtct tatttagtga ttaatcaggt acggtttgaa gaattgcaat
     9121 atagcattaa cctatcccca gacctcgata gtcttgcagt gcctaaattg ttcttgttac
     9181 ctttgataga aaatgccatc aaatatggct tgaaagaacg acacgatgtc aagattaaca
     9241 ttgcttgtta ttatcaagat gaccacatta ttttttctgt gagagataat ggttcgggaa
     9301 ttgatgctca tcaccaaaaa gtgattcgag agcaattgga agctggagag tcacaccatg
     9361 ggttaattaa ctcttatcgt cggcttaagt atcatttttc agaagtatcc ttggtttttg
     9421 atcaaggtga taaacagttt aatgttagct accatgttaa ggagtagtct atgtacaaat
     9481 tagtcattat tgaagatgaa catattatta ggaaatggtt gcgatacgct attgattata
     9541 aagctttaga tattttggtc attggcgagg ctaaagatgg caaagaagga gctgttttaa
     9601 tcaaagagag tcaaccagat atcgttttaa ccgatattaa catgcccatc atgacagctt
     9661 ttgacatgtt tgaagcaaca aaagatcaaa cttacgccaa aattatttta tctggctatg
     9721 ccgattttcc aaatgcaagg tctgccatac actacggtgt acttgaattt ttaacaaaac
     9781 cgattgaaaa agcagctctt tgggaatgtc ttcaaaccat tattgctaaa atagaaaagc
     9841 aaaaggggtc taatcaaaaa acagatgctt gcgtctatat tcccttaccc cagatgactg
     9901 atcagattcc tgaagtagtc aaagatattt tggagtgggt acatgctcat tttcaagaca
     9961 agatcagcac gagccgccta gctcatgact tagggtatag tgagagttat atctatcaaa
    10021 atatcaaaaa gcatcttcag atgcctttaa gtgattacat taatcaatat cggattaatc
    10081 aggcaattca attgatgcaa caagaaccag atttaatggt atatgaaata gcacaagctg
    10141 taggtattta tgactatcgc tattttgata gggtttttaa aaagtatcta ggacaaactg
    10201 taaaggcttt taaagaagag cattttatga aagataccga taggagtaag ccatgttaaa
    10261 actcaaacgc aaatggctat taagcttttt gttagtaata attattttag caatcgttaa
    10321 cgttgcaatg tgtatattct catcgtcaaa aaaagatagt gctaaagaat tagttatctt
    10381 gactcctaat agccaaacta ttttaacagg gactattcca gcctttgagg aaaagtatgg
    10441 ggttaaagta agattaatcc aaggtgggac gggccaactt attgatcaat taggtcgaaa
    10501 agataagcca ttaaacgctg atattttctt tggtggcaat tacactcaat ttgaaagcca
    10561 taaagattta tttgaatcct atgtttctcc gcaggtttct actgtcattt cagattacca
    10621 attgcctagt catcgtgcaa ccccatatac gatcaatggc agtgtactga ttgttaataa
    10681 cgaattagca agaggacttc atattaccag ttatgaggat ttgctacaac cagctttaaa
    10741 aggcaaaatt gcttttgctg acccaaatag ctcatctagc gctttttcac aattaacaaa
    10801 catcttactg gctaaaggag gctatacgag ctcagaagct tgggagtatg tgaaacgttt
    10861 attactgtct atgaattcta tcagagcaac aagttcttca gatgtttacg aatctgttgc
    10921 agaaggtaaa atgattgttg gtttgaccta tgaagatcct tgcatcaatc tgcaaaaaag
    10981 cggtgcaaac gtttcgattg tttatcctga tgaggggaca gtatttgttc cgtcatcagt
    11041 cgctattatc aaaaaagcaa aggcaatgaa agaagctaag caatttatta attttatttt
    11101 atcatcagat gtacaaaatg cctttgggca gtcaactagt aatagaccta tccgacgaga
    11161 tgctcaaaca agtcatgaaa tgaaaccatt agatgaaatt attactttaa aagaagatta
    11221 cggatatgtt actagacata agaaaaaaat attgaatacc tataatcgac tacatgaagc
    11281 attagaaaga ggaaagtaga ctctaggctt gttgttgcta tactagtagt gttacaaaat
    11341 agcgtagtca ttttttgaaa tcgatttcat aatctggtag actaaggtta tcacgaaaag
    11401 gaggctatat gtgatgactt atcaaactat ttacccttat acaaatgaag tccttcatac
    11461 atttgataat atgaccgacc aaggtgtggc agatgtcctt gaaagagctc accttctcta
    11521 taaaaaatgg cgcaaggaag atcatttaga agaacgtaaa gcccagcttc atcaagtggc
    11581 taatattctt cgccgtgacc gcgataaata tgctgaaatc atgaccaaag acatgggaaa
    11641 actgttcaca gaagcccaag gtgaagtaga cctttgtgcc gatattgctg attactacgc
    11701 tgataaggca gatgaatttt tgatgtctac gccactagaa acagattcag gtcaagctta
    11761 ctatttgaaa caatcaactg gtgttattct agccgttgaa ccttggaatt tcccttacta
    11821 tcaaatcatg cgggtttttg cgccaaactt tattgttggg aacccaatgg ttttaaaaca
    11881 cgcgtccatt tgcccacgct ctgcccaatc tttcgaagaa ttagttcttg aagctggtgc
    11941 tgaagcaggt agtattacca atttgtttat ttcttatgac caagtatcac aagtgattgc
    12001 tgataaacgt gtggttggtg tctgcttgac aggttccgaa cgtggaggag ctagcattgc
    12061 agaagaagct ggtaaaaact tgaaaaaaac aacccttgaa ttgggtggag atgatgcctt
    12121 tattatcttt gatgacgcgg attgggatca attagagaag gtgctttact tctctcgtct
    12181 ctacaatgca ggacaagtat gtacctcatc gaaacgtttt atcgtgcttg acaaagacta
    12241 tgaccgtttc aaagaattat taacaaaagt cttcaaaaca gctaaatggg gtgacccaat
    12301 ggatcccgaa acgacgttgg cgccattatc atcagctcaa gctaaagcag atgttcttga
    12361 ccaaattaag ttggccttgg atcatggtgc ggagttggtt tatggtggtg aagcgattga
    12421 tcatccaggt cattttgtca tgccaaccat tattgctggc ctcacaaaag acaaccctat
    12481 ttactatcaa gaaatctttg gcccagtcgg tgaaatctac aaagtgtctt ctgaagaaga
    12541 agcgattgaa gtggcgaatg attctaacta tggccttgga ggtactatct ttagtagcaa
    12601 tcaagaacat gcgaaagccg tagctgctaa aattgaaact ggtatgtctt ttatcaactc
    12661 aggttggaca agtcttccag aactcccatt tggtgggatt aaacattcag gctatggtcg
    12721 tgaattgagt gagcttggct ttacatcttt tgtgaatgaa caccttatct atattccgaa
    12781 taaaacgaat aatagcaata ctaaagtttg atctagattg ttaaaggaga tctgctcgcc
    12841 ttaataaaca atcaaggcgt tacactcttc ttttttaaag tcaaaatttt aaaaatttga
    12901 taaactagta acatgaacga actgattaaa cataaactag aactcttacc agacagccct
    12961 ggctgttact tacataaaga taaagaagga actattatct atgtcggtaa ggcaaagaat
    13021 ttgaaaaaac gcgtccggtc ctattttcgt gggagccatg ataccaaaac agagttacta
    13081 gtatctgaga ttgtagattt cgaatatatt gtaacagagt cagataccga agcgcttttg
    13141 ttggaaatta acttgattca aaaaaatatg cctaagtaca acatcaagtt aaaagatgat
    13201 aaatcttatc cctttctcaa aatcacaaat gagtcttttc ctcggttagt gattacaaga
    13261 tatattaaga aaaatgatgg attgtacttt gggccctacc cagattctta cactgctaat
    13321 gaagttaaaa agttattaga taggattttt ccatttaaaa agtgtaaaaa tcctatcaat
    13381 aaggtctgtt tttattacca tttaggccag tgctgtgctc ataccatttg tcatacagac
    13441 aaggcctatt gggatcgttt aattgatgat gttaagcact ttttaaatgg taaagatgac
    13501 aaaattattg aagatcttcg ttctaagatg ttagcagctt ctgaagaaat ggcatttgaa
    13561 cgtgccgctg aatatcgaga tttgatttca ggtattgcca ccatgcgcac caagcagcgg
    13621 gtgatgagca aggatttgca agatagagat atctttggct attatgttga taagggatgg
    13681 atgtgtgtcc aagttttctt tgtgcgtcaa gggaaattaa ttcagcgtga tgttaacctt
    13741 tttccctatt ataacgatgc agaagaagat ttcttaactt atatggggca attttaccaa
    13801 gataaacagc atttcatccc taaagaagtc tttattccag aagctattga tgaagaactg
    13861 gtagccgcta ttgtgccaac caaaatcatc aaacccaaac gcggtgaaaa aaaacaactg
    13921 gtagctttgg ctactaagaa tgctcgtgtt agtctgcaac aaaaatttga tttattagaa
    13981 aaagatatca aaaaaacgag tggtgctatc gaaaatttag gtcagttact taggattgac
    14041 aagcctgtgc gcattgaagc ctttgataat tctaacattc aagggacaag tccagttgcg
    14101 gctatggttg tttttgttga tggcaaacct agtaagaaag attatcgtaa atttaagatt
    14161 aaaacagtgg tgggaccaga tgattatgcc agtatgagag aagtgctttt tcgaaggtac
    14221 agtcgggtga aaaaggaggg actgcaagct cctaacttga ttattgttga tggaggcgtt
    14281 ggacaagtca atgtggccaa agatgtgata gaaaaacaac ttggactcac tattcctgtt
    14341 gcgggacttc aaaaaaacga taagcaccag acccatgatt tgctttttgg aaacccgctt
    14401 gaagttgtac cgttgccgcg ccgttctgaa gagtttttct tactgcatcg gattcaagac
    14461 gaagtgcatc gctttgcagt tacatttcat agacaagtgc gacgtaaaaa ttctttttca
    14521 tcaacattag atcatatttc agggcttggc ccaaagcgaa aacagttatt actaagacac
    14581 tttaaaacca taacagcgat tgcatcagct acctctgagg agattcaagc tttaggtata
    14641 cctaaaacag tagttgaagc aatacagcaa cagataacag acaataaaaa tgatagatcg
    14701 tctccttaaa taagtgttat gttttcagta ctgatgaggt aaaaaggcgc tacttgttac
    14761 gcacgacatt tcagttcgtt attttgaaac tttcagaaag ttgcgaagtg tagtgagttg
    14821 atgattttaa aaactatttt atggtaaaat aaaatggtat aaaaaaaaca gatttagata
    14881 ggaagttttg aaatgaaatt tctagagtta aacaaaaaac gccatgccat taagaccttt
    14941 aatgaccagc cagttgacta cgaggatttg cgaacagcca ttgagattgc aaccttagca
    15001 cctagtgcca ataacattca accttggaaa tttgtagtcg ttcaagagaa aaaagctgag
    15061 ttagcaaaag ggttacctct agcaaataaa gtgcaggttg aacaggctca atatgtagtg
    15121 gcgctatttt cagacactga tttagcttta cgttctcgta aaattgcccg tatcggtgtc
    15181 aaatcattac cagacgactt aattggttat tatatggaga ctttgccacc acgttttgct
    15241 gcctttaatg aggttcaaac cggtgagtac ttagctatta atgcaggcat tgtggctatg
    15301 aacttggtgt tatccttgac ggatcaaaag attgcttcca atattatcct tggttttgat
    15361 aagtcaacga ccaatgagat tttagacatc gatccgcgtt ttcgtccaga attattgatt
    15421 actgttggct atagtgacga gaaaccagaa ccaagttatc gtttgcctgt tgatgaagtc
    15481 atcgaaagaa gatagagtca ggacaattag ttaaatgtaa tgtccagatg cgctacggag
    15541 cgaccactat tgttagactg atactatttt ggcttatttt aagttaaaca ttatccctaa
    15601 aacgaataag gagagtataa tgacaaccat tgattttaaa gcagaagttg acaaacgtaa
    15661 agaagccatg ttagcagatt tagttgacct attacgcatc aattcagaac gcgatgacac
    15721 attagctgat gacaaacatc cctttggacc aggaccagtt aaggctttag agcatttcct
    15781 cgcaatggct gagcgtgatg gctacaaaac gcgtaatatt gataactatg caggtgactt
    15841 tgaatttggt caaggtgatg aagtactagg aatctttggt caccttgatg ttgtgccagc
    15901 tggtagcggt tgggatacag acccttatga gccagtgatt aaagacgatc gcatctatgc
    15961 gcgtggatct tcggatgata agggaccaac catggcgtgc tactacgctc tgaaaatcat
    16021 taaagaattg ggtcttcctg tgtctaaaaa agttcgtttc attgttggta ccgatgaaga
    16081 atctggctgg ggggatatgg attattactt tgcccataac ggtttgaaaa atccagactt
    16141 tggtttctca ccagatgctg aatttccaat tatcaatggt gaaaaaggaa atatcaccga
    16201 atacctccat ttcgctggtg acaacaatgg tgcttttgtg cttcatcgct tccaaggtgg
    16261 gttgcgtgaa aacatggtac cagaatctgc aacagctgtc atcacgtcac cgcatgacct
    16321 tgatgtgcta gaagcagctt tggagcaatt tttatcagaa catggtgtta aaggttctat
    16381 gaaggctact gatggtaaaa ttgaagtaac tattattggt aagtctgctc acgggtcgac
    16441 tccagaagcg ggtgttaatg gtgccacctt gttggctaaa ttcctgaatc aatttacctt
    16501 tgaaggggct gctaaggatt atttatatgt ggcaggtgag gtgcttcatg aagactttgc
    16561 ggcagaaaaa ctaggccttg cttataccga tgaccgtatg ggagccctga gcatgaacgc
    16621 aggtgtcttc acctttgata gtcaatcagc tgacaatacc atcgccttga atttccgcta
    16681 tccaaaagga actgatgcgg caaccctaaa agcaggattg gaaaaattac caggagtaat
    16741 taaggtaagc ctttctgagc acgaacatac acctcattat gtgccaatgg atgatgaatt
    16801 agtagctacc ttacttgctg tttacgaaaa acaaacaggt cttaaaggct acgaacaagt
    16861 tatcggcgga ggaacctttg ggcgcttgct agaacgtggt gtggcctttg gagcaatgtt
    16921 cccaggtgat gaaaacacta tgcaccaagc taatgaatat atgcctttag aaaatattta
    16981 tcgctcagca gctatttatg ctgaagcaat ctacgaatta attaaataag atgcttatga
    17041 cagcaaaaag agctggaaca ctgttccagc tcttttgtga tgattagaga ctgttgttat
    17101 ttaccaagac agaattgact aaagagttgg gtaatgagtt catcaggagc agcatcccct
    17161 gtgatttctc cgagaatttc ccaagttctg gttaaatcaa cctgtagtaa atcaactggc
    17221 atgccaagcg ccaagccgtc attgacagct tctaaactct ggactgcctt ttcaatcaaa
    17281 gaaatgtggc gggcatttga caggtaagtg gcatcttgtt ctactagtcc agcattatca
    17341 aagaagagct ggttgatacg atcttcgatc agattaatgt tctgattggt taacaccgaa
    17401 ataggaataa ggtccgctgg caactgttcc aattcaatct tttgttccaa atctgtttta
    17461 ttcagcaata taatgcggtt actgtcctga ctgagattta acaaagctcg gtcttggtca
    17521 gtcagttttt cagaagcatt cagcacaaga agaacaaggt cagcttcttg aagagctttt
    17581 ttagaccgtt caactccgat ttgttcgacc agatcatctg tctcacgaat accggccgta
    17641 tcgacaagtt ttagaggaac tcccttgata ttaacgtatt cttcaatgac atctcgggtg
    17701 gttcctgcaa tatcagtcac aatagccttg tcttctcgca acaaattatt aagtaagctt
    17761 gacttaccga cgtttggacg accaataata gctgttgata agccctcacg cagaatttta
    17821 ccgcgtttag ctgttcgaag gagattttcc aaaaggcttt ggaattcttg tgttttctca
    17881 cgtagcagag cagtggtcat ttcttccaca tcgtcgtatt cagggtagtc aatattgact
    17941 tcgacttgag ctaaggtgtt gagaatttct tgtcgggtgt cattgataag ctgggaaagg
    18001 gagccatcca gttgcttgac tgcaatcgtc atagccttgt ctgttttggc acgaataatg
    18061 tccatgacag cctcagcttg agtcaaatca actcgcccat ttaagaaggc acgtttggtg
    18121 aattcgccag gctctgccat tctagcgcct tggcgaatca atagttgcaa gatttcattt
    18181 gtcacagcaa taccaccgtg cgtattgatt tcaaccacat tttcacgggt aaaggttttg
    18241 ggggctagca tgactgacac cataacttca tcgataatag ttcctgtttt aggatcaatg
    18301 atatggccat agttaatagt gtgtgaggcg acttgctcta agttctttcc cttaaaaaca
    18361 gattgtgcaa tggcaagcgc atctgtccct gatagacgaa caatgccaat agctccttca
    18421 ccaagagggg ttgaaatggc ggtaattgta tcaaattctt tagttatact catgccctct
    18481 attttaaatc aagtcctagt agaaagcaag gtattatttt gtatagaaaa taggactagc
    18541 accattttga gtttagctaa tactaaaaaa gaataaatgt aaaaaaatca tttttttaat
    18601 aatttttatt gacaatagta ttataaaaga atataatgat ataggttttg ataaaaaact
    18661 gacctaagac agcaggggag catgctcata atattcctgc cgaggcacaa aacgtgatta
    18721 aagaaaataa cgtatttgta ctttcgtgtc taggtttagg tgcgattttt ttgtacctag
    18781 cccaaaaata aaaacggagg taaaactaat gagtgaagca attattgcta aaaaagctga
    18841 acaagtcgaa cttattgctg agaaaatgaa agcagcagca agtatcgttg ttgttgattc
    18901 acgtggtctt acagttgatc aagatactgt tcttcgtcgt tcacttcgtg aaagtggcgt
    18961 tgagttcaaa gttatcaaaa actcaatttt gactcgtgca gctgaaaaag caggtcttga
    19021 tgagttgaaa gatgtattcg taggaccatc tgcagtagca ttttctaacg aagatgttat
    19081 cgcaccagct aaagttatca acgactttac taaaactgct gacgcacttg aaatcaaagg
    19141 tggtgcaatc gaaggcgctg tttcttcaaa agaagaaatc caagcacttg caacattgcc
    19201 aaaccgcgaa ggaatgcttt ctatgcttct ttctgtactt caagctccag tccgcaacgt
    19261 tgcatacgct gtcaaagctg ttgcagaaaa caaagaaggc gctgcttaag acgtctcaga
    19321 gtagcttaag gctacgctta acattttaaa ttaattattt ggaggaaatc acaatggcat
    19381 tgaacattga aaacattatt gctgaaatta aagaagcttc aatccttgag cttaacgatc
    19441 ttgtaaaagc tatcgaagaa gaatttggtg taactgcagc tgctcctgta gctgctgcag
    19501 ctgctggtgg tgctgaagaa gctgctaaag attcattcga cgttgaattg acatctgctg
    19561 gcgacaaaaa agttggcgtt atcaaagctg ttcgtgaaat cacaggtctt ggtcttaaag
    19621 aagctaaagg tcttgttgat ggagcacctg ctaacgttaa agaaggcgtt gctgctgcag
    19681 aagctgaaga aatcaaatct aaacttgaag aagctggagc aacaatcact cttaaataag
    19741 aagcagatag caatttgaga gcgaaatccc gatttatcag tcttttagag cttatagcga
    19801 tttgaagaaa ctgataaaat gatttggttt tttgccaaaa atcctgccaa aaaaatcttt
    19861 ggcaggattt ttattttcta taaaaattaa gctgagtag
//
LOCUS       DAUDYX010000040        19215 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.040, whole genome shotgun sequence.
ACCESSION   DAUDYX010000040 DAUDYX010000000
VERSION     DAUDYX010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19215
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.040"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(65..1018)
                     /gene="manA"
                     /locus_tag="VHD02_001384"
     CDS             complement(65..1018)
                     /gene="manA"
                     /locus_tag="VHD02_001384"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEQ5497987.1"
                     /translation="MSEPLFLKSTMHDRIWGGTKLRDVFAYNIPSDTTGEYWAISAHP
                     NGVSTVTNGRYQGQPLNTLYAQEPALFGNPKEEVFPLLTKILDANDWLSVQVHPDDAY
                     GREHEGELGKTECWYIISAEEGSEIVYGHQAKSKEDLRAMIEAGAWDDLLTRVPVKAG
                     DFFYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRKDVNGNLRDLHIEKSIDVLTI
                     GKPENSVPATMVLDNMVATTLVSTPFFTVYKWVTSQMVDMKQAAPYLLVSVLKGQGKL
                     YVDQKAYELEKGMHFILPNDVKSWSFDGQLEMIVSHPNQLV"
     gene            complement(1113..1997)
                     /locus_tag="VHD02_001385"
     CDS             complement(1113..1997)
                     /locus_tag="VHD02_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEQ5497988.1"
                     /translation="MGKLYGSIEAGGTKFVCAVGDEEFTVVDKTQFPTTTPEETIAQT
                     IAYFKTFEADLAGMAIGSFGPIDIDPSSETYGYITTTPKSGWANVDLLGQLSAAFKIP
                     FDVTTDVNSSAYGEVLARPGVESLVYYTIGTGIGAGAIQHGHFIGGLGHTEAGHTYVM
                     PHPDDMAKGFLGVCPFHKGCLEGMAAGPSIEARTGVRGERLDQEADVWDIQAFYIAQA
                     ALQATMLYRPQVIVFGGGVMAQEHMVLRVHDKFTTLLSGYLPVPDLTDYIVTPAVADN
                     GSATLGNFALAKLAAQGK"
     gene            complement(2261..5248)
                     /locus_tag="VHD02_001386"
     CDS             complement(2261..5248)
                     /locus_tag="VHD02_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEQ5497989.1"
                     /translation="MDKHLLVKRTLGCVCAATLMGAALATHHDSLNTVKAEEKTVQVQ
                     KELSSIDSLHYLSENSKKEFKEELSKAGQESQKVKEILAKAQQADKQAQELAKMKIPE
                     KIPMKPLHGPLYGGYFRTWHDKTSDPTEKDKVNSMGELPKEVDLAFIFHDWTKDYSLF
                     WKELATKHVPKLNKQGTRVIRTIPWRFLAGGDNSGIAEDTSKYPNTPEGNKALAKAIV
                     DEYVYKYNLDGLDVDVEHDSIPKVNGEASDENLKRSIDVFEEIGKLIGPKGVDKSRLF
                     IMDSTYMADKNPLIERGASYINLLLVQVYGSQGEKGVFQNDTKLVTDTPEERWQGYSK
                     YIRPEQYMIGFSFYEENAQEGNLWYDINSRKDEDTANGINTDITGTRAERYARWQPKT
                     GGAKGGIFSYAIDRDGVAHQPKKYAKQKEFKDATDNIFHSDYSVSKALKTVMLKDKSY
                     DLIDEKDFPDKALREAVMAQVGTRKGDLERFNGTLRLDNPAIQSLEGLNKFKKLAQLD
                     LIGLSRITKLDQSVLPANMKPGKDTLETVLETYKKDNKEEPATIPPVSLKVSGLTGLK
                     ELDLSGFDRETLAGLDAATLTSLEKVDISGNKLDLAPGTENRQIFDTMLSTVSNHVGS
                     NKQTVKFDKQKPTGHYPDTYGKTSLRLPVANEKVDLQSQLLFGTVTNQGTLINSEADY
                     KAYQNQQIAGRSFVDSNYHYNNFKVSYENYTVKVTDSTLGTTTDKTLATDKEETYKVD
                     FFSPADKTKAVHTAKVIVGDEKTMMVNLAEGATVIGGSADKEMSRNVFDGSLGGDRSN
                     LLGMSGPKSTIIFNLKESGIVKHWRFFNDRARSPQNPAEDIQEIKLQVFDSEKYDIET
                     LLKTPNQFDKDDYWITVDSYKSKSETVNNYDQVLKMNSAKYWRVTVDTKGGNYSWPSL
                     PELQILGYPLPNADTIMKTVTIAKGLSQQKDKFSQKMLDELKIKEMALETSLNSKIFD
                     VTAINANAGVLKDCIEKRQLLKK"
     gene            complement(5479..7362)
                     /locus_tag="VHD02_001387"
     CDS             complement(5479..7362)
                     /locus_tag="VHD02_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEQ5497990.1"
                     /translation="MDNRQIAAEVIEALGGRENVRSVAHCATRLRVMVYDEGKIDKEK
                     AEAIDKVKGAFFNSGQYQMIFGTGTVNNIYDEVVALGLPTSSTSEQKAEAGKQGNIFQ
                     RAIRTFGDVFVPIIPAIVATGLFMGVRGLVTQPAIMDLFGVHEYGENFLMYTRILTDT
                     AFVYLPALVAWSAFRVFGGNPIIGIVLGLMLVSNELPNAWVVASGGDVKPLTFFGFVP
                     VVGYQGTVLPAFFVGLVGAKLEKWLHKKVPEALDLLVTPFLTFAIMSTLGLFVIGPVF
                     HSLENLVLAGTQAVLHLPFGIAGLIVGGIQQLIVVTGIHHIFNFLEAQLIANTGKDPF
                     NAYLTAATAAQAGATLAVAVKTKSTKLKGLAFPSTLSALLGITEPAIFGVNLRYPKVF
                     VSGLIGGALGGWVAGLFGIAGTGFGITVLPGTLLYLNGQLLQYLVTMLVGLGVAFAIA
                     YTWGYQDRETLPLPAVEVDQTADQPALAEETLYSPLNGTVVDLAAVSDPVFSSGAMGQ
                     GLAIKPEDNTLYSPVDGKVEIVFETGHAYAITSSQGAEVLLHIGIDTVSMAGDGFESL
                     VAVGQAVKKGDLLGHFDPSKIAEAGLDDTTMMIVTNSADYQSVDILAQGHVLIGDQVA
                     LIK"
     gene            7604..9043
                     /locus_tag="VHD02_001388"
     CDS             7604..9043
                     /locus_tag="VHD02_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEQ5497991.1"
                     /translation="MDLPQAIRYRPYKEWSSKDYQAITEKMAQSPWHSQFHVEPKTGL
                     LNDPNGFSYFNGHYHLFYQNWPYGAAHGLKQWVHMTSTDLVHFTETRSRLLPDHAHDS
                     HGAYSGSAYAIDDKLFLFYTGNVRDANWVRTPLQVGAWMDKQGNISKIPQVLIEQPDD
                     VTEHFRDPQLFSYQGQFYAIIGAQGLDGKGKIKLYKAVDNHVDNWRFIADLDFDDSGT
                     EYMIECPNLVFVDDKPVLIFSPQGLAKADLDYQNIYPNTYKIFESFNPETGQLLGGGV
                     LQNLDFGFEAYATQAFSSPDGRVLAVSWIGLPDIDYPTDRYDYQGALSLVKELRIKDG
                     ILYQTPVSALQNLRGPAELFHNKIDSSNCYELELTIPGQKKLDLLLFTDQKGNGLRLK
                     VDTTKGQLSIDRSRAGVQYAQDYGTVRSCQIPQGHVTLNVYVDNSILEIFINQGQKVL
                     TSRVFPTQGQTGIQVVKGQAFGHYYEMRY"
     gene            9048..10013
                     /locus_tag="VHD02_001389"
     CDS             9048..10013
                     /locus_tag="VHD02_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ5497992.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINKKGYLSQKTVNKVNKAMREL
                     GYKPNNLARSLQGKSTQLIGLIFPNISNIFYAELIEHLEIELFKQGYKTIICNSEHNP
                     VKEREYLEMLAANQVDGIISSSHNLGIEDYERVEAPIVAFDRNLAPNIPVISSDNFEG
                     GKLAAQTLQKHGCQNIVMITGNDNSDSPTGLRQLGFNYQLKRSAEIIKLPNNLSPVRR
                     EMEIKSILATRKPDGLFVSDDLTAILIMKVAKQLHITIPEDMKVIGYDGTTFIQQYVP
                     QLATIRQPIDEIAKLSVEILIKKIKKEKTSKDYILPITLLPGASI"
     gene            complement(10154..10606)
                     /gene="nusB"
                     /locus_tag="VHD02_001390"
     CDS             complement(10154..10606)
                     /gene="nusB"
                     /locus_tag="VHD02_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEQ5497993.1"
                     /translation="MTNSFQNSRRDLRERAFQALFNIEMGAELLAASQFAYGYDKVTG
                     EDAQVLELPIFLLSLVTGVNNHKEELDNLISTHLKKGWSLERLTLTDKTLLRLGLFEI
                     KYFDETPDRVALNEIIEVAKKYSDETSAKFINGLLSQYVSEAPSANKS"
     gene            complement(10599..10988)
                     /locus_tag="VHD02_001391"
     CDS             complement(10599..10988)
                     /locus_tag="VHD02_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ5497994.1"
                     /translation="MTTEYIGEIVISPRVLEVITGIATTQVEGVHSLHNKKMADSFNK
                     ASLGKGVYLQTEEDGSVTADIYVYLQYGVKVPTVSMNIQKTVKSAVYDMAEVPISAVN
                     IHVEGIVAEKTPKPDLKSLFDEDFLDD"
     gene            complement(11034..11591)
                     /gene="efp"
                     /locus_tag="VHD02_001392"
     CDS             complement(11034..11591)
                     /gene="efp"
                     /locus_tag="VHD02_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEQ5497995.1"
                     /translation="MIEASKLKAGMTFEAEGKLIRVLEASHHKPGKGNTIMRMKLRDV
                     RTGSTFDTTYRPDEKFEQAIIETVPAQYLYKMDDTAYFMNTDTYDQYEIPVANVEQEL
                     LYILENSDVKIQFYGSEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATLETGLVV
                     NVPDFIEAGQKLIINTAEGTYVSRA"
     gene            complement(11687..12148)
                     /locus_tag="VHD02_001393"
     CDS             complement(11687..12148)
                     /locus_tag="VHD02_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEQ5497996.1"
                     /translation="MTNRLSWQDYFMANAELISKRSTCDRAFVGAVLVKDNRIIATGY
                     NGGVSATDNCNEAGHYMEDGHCIRTVHAEMNALIQCAKEGISTDGTEIYVTHFPCINC
                     TKALLQAGITKITYKAHYRPHPFAIELMEKKGVAYVQHDVPQIVLGGDKEQ"
     gene            complement(12183..13256)
                     /locus_tag="VHD02_001394"
     CDS             complement(12183..13256)
                     /locus_tag="VHD02_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEQ5497997.1"
                     /translation="MSGFLEQRLGHCLRQMAEKGLEALLVTHLTNSYYLTGFSGTAAT
                     VLITAKRRVLITDSRYTLLAKASVEGFDIIESRTPLKVVAELLEADQIDCLGFEDQVS
                     FSFYQAMQAELSGITLLAQSGFVEHLRFIKDTSEIDTIAKACSISDKAFEDALDFIKP
                     GTTTERDLANFLDFRMRQYGASGTSFDIIVASGYRSAMPHGRASDKVIQNKESLTMDF
                     GCYYNHYVSDMTRTIHIGQVTDEEREIYALVLAANKALIAKAIAGMTYSDFDGIPRQL
                     ITEAGYGSHFTHGIGHGIGLDIHENPFFGKSEQLLQAGMVVTDEPGIYLDNKYGVRIE
                     DDLVITKTGCQVLTLAPKELIVL"
     gene            complement(13372..16200)
                     /gene="uvrA"
                     /locus_tag="VHD02_001395"
     CDS             complement(13372..16200)
                     /gene="uvrA"
                     /locus_tag="VHD02_001395"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEQ5497998.1"
                     /translation="MQNKIIIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSAEQIVEQVLALPERTRMQILAPIVRRKK
                     GQHKTIFEKIQKDGYVRVRVDGDIFDVTEVPELSKSKMHNIEVVIDRLVNKDGIRSRL
                     FDSVEAALRLGDGYLMIDTMDGNELLFSEHYSCPVCGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDLDLVVPDPSKSLREGALAPWNPISSNYYPTMLEQAMASFGVDMDTP
                     FEALTEEERDLVLYGSGDREFHFHYVNDFGGERNIDIPFEGVVTNVNRRYHETNSDYT
                     RNVMRGYMNELTCATCHGYRLNDQALCVHVGGEEGPHIGQISELSIADHLQLLEELEL
                     TENESTIAKPIVKEIHDRLTFLNNVGLNYLTLSRAAGTLSGGESQRIRLATQIGSNLS
                     GVIYILDEPSIGLHQRDNDRLIESLKKMRDLGNTLIVVEHDEDTMMQADWLIDVGPGA
                     GEFGGEIIASGTPKQVAKNKKSITGQYLSGKKFIPVPLERRSGNGRFIEIKGAAQNNL
                     QSLDVRFPLGKFIAVTGVSGSGKSTLVNSILKKAVAQKLNRNADKPGKYHSISGIEHI
                     ERLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVPCEVCHGRRYNSETLEVHYKEKNIAEVLDMTVDDALV
                     FFSAIPKIARKIQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHTDDIARLLKVLERFVDDGNTVLVIEHNLDVIKSADHIIDLGPEGGVGGGQ
                     IVATGTPEEVAQVKESYTGHYLKVKLQQ"
     gene            complement(16414..16596)
                     /locus_tag="VHD02_001396"
     CDS             complement(16414..16596)
                     /locus_tag="VHD02_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEQ5497999.1"
                     /translation="MLTYDEFKQAIDHGYITGDTVAIVRKNGQIFDYVLPHEKVKNGE
                     VVTEEIVEEVMVELDK"
     gene            16829..17815
                     /locus_tag="VHD02_001397"
     CDS             16829..17815
                     /locus_tag="VHD02_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEQ5498000.1"
                     /translation="MDMSKKLRNSLARIVVAAFASFAVMAIPTYHHSTVLAKTVSVNQ
                     TYGEYKDYYTVIGESNIDQSAFPKIYKTTERVYKGQGTSEKRVTVSDVVYNPLDGYKR
                     STGAYGVVTKDMIDMSKGYREKWETNPEPSGWFRFYNRADNEEISEKEYDSRRTKSYK
                     VTNNVPVVLTTLKGKQYNSHLFVRSHLFADSLGGKSIRKNAITGTQMQNVGTRKGGMQ
                     YIEKKVLSHITKNPDVYVFYSAIPEYQGTELLARSVLVSALSSDGVINETVRVFNTAD
                     GFNINYEKGGLLTESPVSEIDNIEDSTTDEIENSVDDSEEIVYNDTTTEEEN"
     gene            complement(17929..18444)
                     /locus_tag="VHD02_001398"
     CDS             complement(17929..18444)
                     /locus_tag="VHD02_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498001.1"
                     /translation="MKSKKLVFGQIDLNMCRDFDLVQALDYDFKEKKLYNKGRGFAIV
                     LVSIQNLTFAIPLRSNIPKKYQLKYKLRDSKKYGCVEGLDIGKALVVEDPKYILNRTF
                     KLREQVDYFKIVDNDILIVNKLIKAIIDYNQAVANSDQHKLTDPKRFKFSTFPNYTDR
                     LKSITDADYLR"
     gene            complement(18766..>19215)
                     /locus_tag="VHD02_001399"
     CDS             complement(18766..>19215)
                     /locus_tag="VHD02_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEQ5498002.1"
                     /translation="IEDSDGYTMRTVEQNIDGNADALYVGAPARFNTRDFTGVVGWFY
                     PPYQGDTVTQPVSTEPQTSDTIVETAKTGTFTLDVAEINIRRWPSLASEVVGIYKQGD
                     TVSFDSEGYANGYYWISYVGGLGMRDYLAIGQTDKDGNRISIWGKLN"
BASE COUNT         5736 a         4199 c         3448 g         5832 t
ORIGIN      
        1 taaagttatc agagaaacag agagtcataa ttttatcctt ttttctcata ttgataccat
       61 ggtattaaac gagttgattg gggtgactca caatcatttc taattgacca tcaaatgacc
      121 acgatttaac atcgttaggc aagataaagt gcatgccttt ttctagctca taagccttct
      181 ggtccacata gagttttcct tgacctttta aaacactcac taaaagataa ggcgcagctt
      241 gtttcatgtc aaccatttga ctagtcaccc atttgtaaac agtgaaaaat ggcgttgaga
      301 ctaaagttgt tgctaccata ttatccaaaa ccatagtggc tggcacgcta ttttctggct
      361 taccaatcgt taagacatca atagactttt caatatgaag gtcacgaaga ttaccattaa
      421 cgtccttacg gtcaaaatca taaacccgat aagtggtatc agatgactgc tgggtttcta
      481 aaataagaat tcccttgccg atcgcatgca tggtgccact tggaacatag aaaaaatcgc
      541 ctgctttgac tgggacacga gtcagcaaat catcccaagc acctgcttca atcatggcac
      601 gaaggtcttc tttggacttg gcctgatggc cgtatacaat ttcagaaccc tcttcggcag
      661 aaataatata ccagcattct gtcttgccta attctccttc atgttcacgt ccataagcat
      721 catcaggatg aacttggaca cttagccagt cattagcatc taaaatctta gttaacagtg
      781 gaaaaacttc ttcctttgga ttcccaaaca aggccggctc ttgcgcatat aatgtgttta
      841 aaggttgtcc ttgataacgt ccattagtaa ctgtcgaaac accattagga tgagcagaaa
      901 tagcccaata ctcacctgtg gtgtcacttg ggatgttgta agcaaagaca tctctcaact
      961 tggtgccacc ccaaatcctg tcgtgcatgg ttgattttag aaataatggt tctgacataa
     1021 tattcctcgc ttctgatgtt aatctctgtt tattatagca tgttctagct gactgcgctt
     1081 tcttttacta gacaagcagg caaaaacaga ccctattttc cttgtgcagc caacttggcc
     1141 aaagcaaaat ttcccagagt agcggagcca ttatcagcaa cagctggagt aacaatgtag
     1201 tctgtcaaat cagggactgg caaataaccg cttaaaaggg tagtgaattt atcatgcacg
     1261 cgcaacacca tatgttcttg cgccatgaca cctccgccaa aaacaatcac ttgaggacgg
     1321 taaagcatag ttgcttgcag cgctgcttgg gcaatgtaga aggcttgaat gtcccaaaca
     1381 tcagcttcct gatcaagtcg ttcacctctc actcccgttc tggcctcaat acttggacct
     1441 gctgccatcc cttccagaca ccctttatga aaagggcaaa cacctaaaaa tcccttggcc
     1501 atatcatcag gatgaggcat gacataggta tgacccgctt ctgtatgacc taatccccca
     1561 ataaagtggc catgttgaat agctcccgcc ccaataccag tacctatggt gtaatagacc
     1621 aaactttcca caccaggacg agcaagcacc tcaccataag cagagctatt aacatctgtt
     1681 gtcacatcaa aaggaatctt aaaagcagcc gaaagttgtc ctaacaaatc cacattagcc
     1741 cagcctgact taggagtggt tgtgatgtaa ccgtaagtct ctgacgacgg atcaatatca
     1801 attggtccaa aagaaccaat cgccattcca gccaaatcgg cctcaaaggt tttaaaatat
     1861 gcgatggttt gcgcgatggt ttcttctggt gtggtggtcg gaaattgtgt tttatccacc
     1921 actgtaaact cctcatcacc cacggcacag acaaactttg tcccaccagc ttcaatgctt
     1981 ccatataatt ttcccatgtt ttctcctttt tcttgttctt ttttatcttt atgatgacac
     2041 aggctcttat caagacagcc atgtctcacg gacaagcgcc actttctggt agtctaacga
     2101 ttatttagct ttatgtcatc aaaacctata aaagactaaa aatctgggac tattcatctc
     2161 agatttttag tcttttggtt aatcaattcc agcaaacctt tatcatagtc acattttgtt
     2221 aatttaatct gaatgttgct atctaagaaa gttgctttgt ttattttttt agcagctgcc
     2281 ttttttcaat acaatctttc aaaactccag cattagcatt aatagcagtt acatcaaaaa
     2341 tcttactgtt caaagaagtt tctaaagcca tctcttttat ttttaactca tcaagcatct
     2401 tttgagaaaa cttatctttt tgttgagata gacctttagc aatagttact gttttcatga
     2461 tagtgtcggc gttaggtaac ggataaccta aaatttggag ttcagggagt gatggccaac
     2521 tataatttcc tcccttagta tctacggtta ctctccagta tttggctgaa ttcattttca
     2581 aaacttgatc ataattatta acagtttcgg attttgattt ataactatca accgtaatcc
     2641 agtaatcgtc tttatcaaat tgattaggcg tctttaatag tgtttcaata tcgtattttt
     2701 ctgagtcaaa gacttggagt ttaatttctt ggatgtcttc ggccggattc tgaggtgacc
     2761 gtgctctatc attaaaaaat ctccagtgtt ttactattcc agattctttt aaattaaaaa
     2821 taattgtaga ttttggtcct gacatgccta gtagattaga tcggtctcct cctaaagaac
     2881 catcaaatac gtttctagac atttccttat cagcacttcc tccaataact gttgcgcctt
     2941 ctgccaaatt aaccatcatg gttttttcgt caccaacaat cactttagca gtatgaacag
     3001 cttttgtctt atctgctggg ctaaagaagt caaccttata ggtctcttct ttatcagttg
     3061 ctagcgtttt gtcagtagtg gttcccaatg tggaatcagt tactttaacg gtatagttct
     3121 cataagaaac tttaaagtta ttgtaatgat agtttgaatc aacaaagcta cgtccagcaa
     3181 tctgctgatt ttggtaagcc ttatagtctg cttcgctatt gattagggtt ccttgattcg
     3241 tcacagtccc aaacaaaagc tggctttgca aatcaacttt ttcatttgcc actggtaagc
     3301 gcagactagt tttcccatag gtatctgggt aatgcccagt tggtttttgc ttgtcaaatt
     3361 tcactgtttg tttattgctt ccaacatgat tgctgacagt tgatagcata gtatcaaaaa
     3421 tttgtcgatt ttctgttcct ggagccaaat caagtttgtt gccagaaata tcaacttttt
     3481 ctaaagacgt tagagtagcg gcatcaagac cagccaaggt ttcacggtca aaacctgaca
     3541 aatctaattc tttcagacca gttaaaccag aaaccttcaa agatactggt gggatagtag
     3601 caggttcttc tttgttatcc tttttatagg tttcaagaac tgtttccaag gtatctttgc
     3661 ctggcttcat attagcgggt aaaacagatt ggtcgagctt tgtaatgcga gataagccaa
     3721 tcaagtctaa ttgagctaat tttttaaatt tatttagacc ttctaaactt tgaatcgctg
     3781 gattatccaa tcgtaatgtg ccattgaaac gttccaaatc accttttctg gttccaacct
     3841 gcgccatcac agcttctcgc aaagccttat ctgggaaatc tttctcatca atcagatcat
     3901 acgacttatc ttttagcata actgtcttta atgccttgga gacactataa tctgagtgga
     3961 agatgttatc agttgcgtcc ttaaactctt tctgtttagc atatttttta ggttgatgag
     4021 ctacaccatc tcggtcaata gcgtaggaga agatacctcc cttagcccca cctgtcttag
     4081 gttgccacct tgcataccgt tcggcacgcg ttccagttat gtcagtgtta attccatttg
     4141 ccgtgtcctc gtccttgcga gaattaatat cataccaaag attcccttct tgagcatttt
     4201 cctcatagaa agaaaaacca atcatgtatt gttcaggacg aatatacttg ctataacctt
     4261 gccatcgttc ttcaggggta tcggtgacta atttggtatc attttgaaag acacctttct
     4321 ctccttgtga accatagacc tgtaccagta ataaattaat ataagaagct cctcgctcaa
     4381 tcaatgggtt tttatcagcc atataggtgc tatccataat aaataaccgc gatttatcaa
     4441 caccttttgg tccaattaat ttcccaattt cttcaaaaac atcaatagat cgcttgagat
     4501 tttcgtcact agcttctcca tttacttttg gaatactatc atgttcaaca tccacatcta
     4561 agccatcaag gttgtactta taaacatatt catcaacaat agctttggct aaagctttat
     4621 ttccctctgg tgtatttggg tatttactgg tatcttctgc aataccactg ttatcacctc
     4681 cagctaggaa acgccatgga atggtacgaa tgacacgtgt cccttgcttg tttaactttg
     4741 gcacatgttt ggtggccaat tctttccaaa aaaggctata atcttttgtc caatcgtgga
     4801 aaataaaggc tagatctact tctttaggaa gctctcccat cgagttaact ttgtcttttt
     4861 ctgttggatc tgatgttttg tcatgccaag ttctaaagta accaccgtag agaggaccat
     4921 gtaacggttt catcggtatt ttctcaggaa ttttcatttt ggcaagttct tgagcttgtt
     4981 tatctgcctg ctgagctttt gctaatatct ctttgacctt ttgagattct tgccccgctt
     5041 ttgagagttc ttctttaaat tcttttttgc tattctctga cagataatgc aagctatcta
     5101 tagaagataa ttctttctga acctgaacag tcttctcctc cgcttttaca gtattgagtg
     5161 aatcatggtg ggtcgctaag gcagctccca tcaacgttgc agcacaaaca caccctagtg
     5221 ttctttttac caacaaatgt ttatccattt ggacactcct tatttttggt actaagtcat
     5281 ggcttcccag ttgatatacc atctatgtaa gcgcattcct atagttcgcc ttaagtataa
     5341 caacacttta ttaaaaaaaa caaggtacaa agtataggta aaataacaca aatttaaggt
     5401 ttttaggctt ttctcatttt tttgccaacc tgccattcat ggcaggacaa aagagctgct
     5461 tagttagctc ttttgtcctt atttgataag tgccacttga tcaccaatca aaacatgtcc
     5521 ttgagcaaga atgtccacgc tttgatagtc agcgctattg gtcacaatca tcatggtggt
     5581 gtcatctagt ccagcttctg caatcttgct aggatcaaag tgtccaagaa gatcaccttt
     5641 tttaactgcc tgacctactg ctacaagaga ttcaaagcca tctcctgcca tcgacacggt
     5701 atcaatacca atatgaagta acacttctgc tccttgactt gacgttatag cataggcatg
     5761 acctgtttca aagacaattt caactttacc atcaactggt gagtataggg tattatcttc
     5821 tggcttaatt gctaagcctt gacccatagc acctgatgaa aaaacagggt ctgaaacagc
     5881 tgctaaatct acaactgttc cattcaaagg actatataaa gtttcctctg ctaaggctgg
     5941 ctgatcagcc gtttgatcaa cttcgacagc tggtaacgga agggtttctc tatcttgata
     6001 accccaagta taagcaattg caaaggctac cccaagaccg acaagcatag tcacaaggta
     6061 ttgtaataat tggccattca agtaaaggag agttcctggt aaaaccgtaa tcccaaaacc
     6121 agttcctgca atgccaaaga gcccagcgac ccaaccacct aaggcaccac caataagacc
     6181 tgaaacaaag acttttggat aacggaggtt gaccccaaaa atagctggtt cagtaatccc
     6241 taaaagagct gacaaggttg atggaaaggc cagacctttt agttttgttg atttggtttt
     6301 aacagcaaca gctaaggtag ctccagcttg agcagctgtt gctgctgtta ggtaagcatt
     6361 gaaaggatct tttccggtat tggcaatcag ctgcgcttct aggaagttaa agatatggtg
     6421 aataccagta acgacgatca actgttggat accaccaaca ataagaccag caatgccaaa
     6481 cggcaaatgc aagacggcct gtgtcccagc cagaacaagg ttttcaagag aatggaaaac
     6541 tggtccaatc acaaatagtc ccaaggtact cataatagca aacgttaaaa acggtgtgac
     6601 caataagtct aaagcttctg gaaccttttt gtgcaaccat ttctccaact tagctcctac
     6661 cagaccaaca aagaaggcgg gtaaaacggt accttgataa ccaacaacag gaacaaatcc
     6721 aaagaaggtt agcggcttaa catctccacc agaggcaact acccaggcgt ttggcaattc
     6781 attggacacc aacatcaagc ctaaaacaat accgataata ggattacccc caaagaccct
     6841 aaaggctgac caagccacca aagctggcaa gtagacaaag gctgtatccg ttagaatacg
     6901 agtgtacatg agaaaatttt ccccgtactc atgcacccca aataaatcca taatagctgg
     6961 ctgggtcacc aaaccacgaa cccccataaa gagacccgtt gctacaatag ctggaataat
     7021 gggaacaaag acatccccaa acgtacgaat cgcccgttgg aagatattgc cttgtttgcc
     7081 tgcttctgct ttttgctcac tggttgatga tgttggtaaa ccaagagcaa cgacttcgtc
     7141 ataaatgtta ttgacggtac cagttccaaa aatcatttga tattgaccag agttaaagaa
     7201 agctccttta accttgtcaa tagcttctgc tttttcctta tcaatctttc cttcatcata
     7261 aaccatcacg cgaaggcgag ttgcacagtg ggcaacactt ctcacatttt ctcggccacc
     7321 taaagcctca atcacttcag ctgcaatctg acgattatcc atgcctaaaa gtctcctttt
     7381 tcatttggtg ttagtccctc atccattctg ccagctttgt ggctaagaat gtgcatgacc
     7441 aacgctagtg aatccgcttc acttcttgac tatcatttta tcactaatat tttttatgtc
     7501 aagcgtttaa catattttta ttttatatga tttaaattct tgatttttaa acagaaaacg
     7561 tttagtatat tcttgaagac gactataaaa caaggagatt ttgatggatc taccacaagc
     7621 tattcgctac cgcccttaca aagagtggag ctcaaaagac taccaagcta ttactgaaaa
     7681 aatggcccaa tccccatggc acagccaatt tcatgtcgaa cctaagacag gattactcaa
     7741 tgaccccaat gggttttctt atttcaacgg tcactaccat cttttttatc aaaactggcc
     7801 ttatggggct gcgcatggtc tgaaacaatg ggtacacatg acatccacag acctcgtcca
     7861 ttttacggaa acaaggagcc gccttctgcc agaccacgct cacgatagtc atggggctta
     7921 ctcaggttct gcctatgcta ttgatgacaa gctcttcctc ttttacactg gtaatgttcg
     7981 cgatgccaat tgggtcagaa caccattaca ggttggcgca tggatggata aacaaggaaa
     8041 tatttccaaa ataccacagg ttttgattga acagcccgat gatgttacag aacattttcg
     8101 ggatcctcag cttttttctt atcaaggtca attctatgct attattggcg cccaaggttt
     8161 agatggcaaa ggaaaaatca aactttacaa agctgtggac aatcatgtgg acaactggcg
     8221 atttatagcc gatttggact ttgacgattc tggtacggaa tacatgattg aatgccccaa
     8281 ccttgtgttt gtcgatgaca agcctgtttt gattttcagc ccacaaggac tggctaaagc
     8341 tgatttagat tatcaaaata tctaccccaa tacctataaa atttttgaaa gcttcaatcc
     8401 agaaactggc caattgctgg gtgggggggt ccttcaaaat cttgactttg gttttgaagc
     8461 ttatgccact caagccttta gtagtccaga tggcagggtt ctagccgtgt cttggattgg
     8521 gttaccagat attgattatc cgacagatcg ttatgactat caaggagctc ttagtttagt
     8581 gaaagaatta aggattaaag acggaattct ctatcaaaca cctgtttcag ccctacaaaa
     8641 tcttcgaggg ccagcagaat tatttcataa taaaatagac agttctaact gttatgaatt
     8701 ggaattgacg attccaggcc aaaaaaagct tgacctgctc ctatttactg accaaaaagg
     8761 gaatggccta agattgaaag tggatacaac aaaaggacag ttaagcattg accgaagtcg
     8821 ggcaggggtt caatatgccc aagactatgg tacggtgcgc tcttgccaaa tcccacaagg
     8881 ccatgttaca cttaatgtct atgtggacaa ttccattctt gaaatcttta ttaaccaagg
     8941 gcaaaaagtc ctaactagtc gtgttttccc aacccaagga caaacaggta tccaagtagt
     9001 aaaaggacaa gcttttggac attattatga aatgaggtat taggcaagtg gtcgcaaaat
     9061 taacagacgt ggcagccttg gcgggtgtca gtccaacaac tgtttcgcga gtaatcaaca
     9121 aaaaaggcta cctttcccaa aaaacagtga ataaggtgaa taaggccatg cgtgaattgg
     9181 gctacaagcc taacaatctc gctagaagtt tgcaaggtaa atcaacccaa ctcattgggc
     9241 ttatcttccc caacattagc aatatctttt acgctgaatt aattgaacat ttggaaattg
     9301 aattgtttaa acagggctac aaaactatta tttgcaatag tgaacataat cccgtcaaag
     9361 aacgcgaata cttagaaatg cttgcggcca atcaagtgga cggcattatt tcttccagtc
     9421 ataatttggg aattgaggat tatgagcgtg tcgaagctcc tattgtagct tttgaccgta
     9481 atctggcgcc aaatattccg gttatttctt cagataattt tgaaggtggt aaattagccg
     9541 cacaaacttt gcaaaaacac ggttgtcaaa acattgtcat gattacaggt aatgacaatt
     9601 ctgattcgcc cacagggttg cgccagctag ggtttaacta ccagctcaaa cgaagtgcag
     9661 aaatcatcaa attaccaaat aacctatctc ctgttagacg cgaaatggaa attaaatcca
     9721 ttttggcaac ccgaaaacct gatggccttt tcgtttctga tgatttaact gctatcctca
     9781 ttatgaaagt agccaaacaa cttcacatta ctattccaga ggatatgaaa gtcataggtt
     9841 atgatggaac gacatttatc cagcaatatg ttcctcaatt agcgaccatt cgtcaaccta
     9901 ttgatgagat tgctaaattg agtgtcgaaa tactcatcaa aaaaattaaa aaagaaaaaa
     9961 caagtaaaga ctatatttta ccgattacgc tacttccagg agcaagtatt taaacaaaac
    10021 ataaccaatc actaattact ctaaaagaca aacaagcata ctctttctag atgcgagagt
    10081 atgcttgttt gtcttttaat attcgtacat aaattgaaaa aatcggatac aatcctaaga
    10141 ttgctccgac ttattatgat ttatttgctg aaggagcttc cgagacatat tggctaagta
    10201 agccattaat aaatttagca gaggtttcat cagaatattt tttagctact tcaatgattt
    10261 cgtttagtgc aacacggtct ggtgtttcgt caaaatactt gatttcaaaa agacctagtc
    10321 gtaataacgt tttgtctgtt agtgtcaagc gttctaacga ccaacctttt ttcaaatgag
    10381 ttgatatcaa gttatccaac tcttctttat gattattgac acccgtcaca agacttaata
    10441 aaaaaatcgg cagctccaat acttgagcgt cctcccctgt tactttatca taaccatagg
    10501 caaactgaga agctgccaat aactcagctc ccatctctat gttaaataaa gcctgaaagg
    10561 cacgttcacg caaatctctt cttgaatttt gaaagctgtt agtcatccaa gaaatcctca
    10621 tcaaaaagtg acttcaaatc tggttttggt gtcttttccg caacaatacc ttcaacatgg
    10681 atattaactg cagaaatagg cacttctgcc atatcgtaca cagcagattt gactgttttt
    10741 tgaatattca tagatacagt cgggacctta actccatatt gcaagtaaac ataaatatct
    10801 gctgtaacac ttccatcttc ttctgtctgg agataaaccc ctttaccaag acttgctttg
    10861 ttaaagctat ctgccatttt cttgttatgg agtgagtgga caccctcaac ttgtgtcgta
    10921 gcaatccccg taataacctc aagcactcgt ggtgaaatga cgatttcacc gatatattca
    10981 gttgtcatag catttccttt ctctttttta gactaatctg ttttgacaaa ggattaagca
    11041 cgagaaacgt aagtaccttc tgcagtgtta atgattagtt tttggccagc ttcgataaag
    11101 tctggaacgt taacaacaag tcctgtctca agagttgcag gtttccctga acccgtcacc
    11161 gtcgctcctt taatagatgg ttgtgtttcc gcaacggtca attcaacagt tgttggaacc
    11221 gttaccccaa tcacttcact tccataaaat tggattttca cgtctgagtt ttcaagaatg
    11281 taaagcaatt cttgctcaac gttagcaact ggaatttcgt actgatcata agtatcagtg
    11341 ttcatgaagt aagcagtgtc atccattttg tataggtatt gtgctgggac agtttcaatg
    11401 atggcttgct caaatttttc atctgggcgg taagttgtgt caaaagtaga acctgtacgc
    11461 acatcacgta gtttcatacg catgatagtg tttcctttac ctggtttgtg gtggctagct
    11521 tcaaggacac ggattaattt tccttctgct tcaaatgtca tacctgcttt aagcttactt
    11581 gcttcaatca ttattattac ctcttactaa aatatttatc acttcatttt accacaaaag
    11641 tggccttggc tcaaatcttt tatgaccgtc cttttaggta cactttctat tgttccttat
    11701 cgcctcctaa aacaatttga ggcacatcat gttgcacata ggcaacccct tttttctcca
    11761 tcagttcaat ggcaaagggg tgcggacggt aatgtgcttt ataggtaatc tttgtaatcc
    11821 ccgcctgcaa aagcgcctta gtgcagttaa tacaaggaaa atgagtcacg taaatttcag
    11881 ttccgtccgt tgaaattcct tctttcgcac attgaatcaa agcattcatt tcagcatgaa
    11941 cagtgcgaat acaatgtccg tcttccatat aatgacccgc ttcgttgcag ttatccgtag
    12001 cagaaactcc gccattgtaa cctgtggcaa taatgcggtt atcctttacc aaaacagctc
    12061 ccacaaaggc gcgatcacag gtagagcgct tggaaatcag ctctgcattg gccataaaat
    12121 agtcttgcca tgataaacga tttgtcattg cttctcccct aatcctttcc tgaaccattc
    12181 ttttacaata caattaattc tttgggtgcc aaggtcaaga cttgacaacc agtttttgtg
    12241 ataaccaagt cgtcttcgat acggacacca tatttgttat ccaaatagat acctggctcg
    12301 tctgtgacca ccattccagc ttggagaagt tgctcagatt tcccaaaaaa tggattctcg
    12361 tggatatcaa gcccgatacc atgaccaatg ccatgtgtga agtgactgcc ataacccgcc
    12421 tcagtgatga gttggcgcgg aataccgtca aagtcactat aagtcatgcc agcgatagct
    12481 ttagcaatta aagccttatt agcagcaaga acaagagcat aaatctcacg ttcttcatca
    12541 gtaacttggc caatatgaat ggtcctcgtc atatcactaa catagtgatt gtagtaacac
    12601 ccaaagtcca tggtcaagct ctctttattc tggataacct tgtcactggc gcgtccatga
    12661 ggcatggcag agcgatagcc tgaagctaca atgatatcaa atgatgtgcc gctggcacca
    12721 tactgacgca tacgaaaatc taaaaaatta gccaggtcac gttcagtggt tgtccctggt
    12781 ttaataaaat caagagcatc ttcaaatgct ttgtctgaga tcgagcacgc tttagcaatg
    12841 gtatcaattt cagaagtatc cttaataaaa cgtaaatgct ccacaaaacc tgactgagca
    12901 agcaaggtta ttcctgacag ttctgcttgc atggcctgat aaaaagaaaa cgatacctgg
    12961 tcctcaaaac caaggcaatc tatttgatca gcctctaaca attctgccac aaccttaagc
    13021 ggcgtgcggc tttcgataat atcaaatccc tcaacactag ctttagcaag caaggtataa
    13081 cgtgaatctg tgatcaaaac acgacgtttg gccgttatca aaacagttgc tgcagttcca
    13141 gaaaaacctg tcaagtaata actattggtt aaatgggtga ctagaagagc ctctagcccc
    13201 ttctctgcca tctgccttag gcagtgacct aatcgttgct ctaaaaatcc tgacatcgcg
    13261 aactcctttg gtaacttttg agaatattgt agcatgaaaa aactacttta agtttaccat
    13321 gatttgagag catttaaaag attaaaaagg agctcaaacg aactcctaat cctattgttg
    13381 tagttttact ttcaaataat gaccagtata gctttcttta acttgagcta cctcttctgg
    13441 tgtccccgtg gcaacaatct gaccacctcc gacaccacct tctggtccta agtcaataat
    13501 atggtcagca gatttgataa cgtccaaatt atgctcaatc acaagaaccg tattaccatc
    13561 gtctacaaag cgttccaaaa cttttaatag ccttgcaata tcatctgtgt gtaaaccagt
    13621 cgttggctca tctaggatat acaagctctt acctgtcgag cgtttgtgca attcactagc
    13681 taatttcatc cgctgcgctt ctcctccaga tagagtagta gctggttgac ccaaggtcac
    13741 ataacccaaa ccaacatcct taatggtttg aatttttcgg gcaattttag ggatagcaga
    13801 gaaaaatacc aaggcatcat cgacagtcat gtcaagtact tcagcaatat ttttttcttt
    13861 ataatgcacc tcaagcgtct cagaattata ccgtctgccg tggcaaacct cacaaggcac
    13921 ataaacatct ggcaaaaagt gcatttcgat tttgataatc ccatcacctg aacaagcctc
    13981 acaacgccca cctttgacat taaaagagaa acggcctttt ttgtagccac gaatcttagc
    14041 ttcgttggtt tgggcaaaca agtctcgaat atcatcaaag acacccgtat aggtggctgg
    14101 atttgaccgt ggcgtccttc caatcggact ttggtcaatg tcaatcaagc gttcaatatg
    14161 ttcaatacca gaaatagagt gatatttacc aggcttatca gcattacggt ttaatttttg
    14221 ggcgacagcc tttttcaaaa tactattaac caaggttgat ttccctgagc ctgatacccc
    14281 tgtgaccgca ataaatttac ccagtgggaa tctcacgtcg aggctttgaa gattattctg
    14341 cgctgctcct tttatttcaa tgaatcgccc attaccagaa cgacgctcta atggcactgg
    14401 aatgaatttt ttaccagaca aatattgacc cgtgatggat tttttattct tagccacttg
    14461 tttcggcgtt ccagaggcaa taatttctcc accgaactca cctgccccag gccccacatc
    14521 aatcagccag tctgcctgca tcatggtgtc ttcatcgtgt tctaccacaa ttaaggtatt
    14581 acccaaatca cgcatttttt tcaggctttc gatcaaacgg tcattgtctc tttggtgcaa
    14641 tccaatagaa ggctcatcca agatatagat cacccctgat aagttagaac caatttgtgt
    14701 cgctaacctg attcgttgac tttcaccacc tgataaagtc ccagcagccc gtgacaaggt
    14761 cagataattc agtcccacat tatttaagaa ggtcaggcgg tcatgaattt ctttgacaat
    14821 cggtttagca atggtacttt cattttctgt gagttccaat tcttccaaca actgcaagtg
    14881 gtcggcaatc gataattctg aaatttgacc aatatgaggt ccttcttcgc cacccacatg
    14941 cacacacaag gcttggtcat tcaaacggta accatgacaa gtcgcacagg tcaattcgtt
    15001 catataacca cgcatcacat tgcgcgtata atcactatta gtttcgtgat aacgacgatt
    15061 aacattagtc acaacacctt caaaaggaat atcaatgtta cgctcaccac caaaatcatt
    15121 cacatagtga aaatgaaatt ctctatctcc tgaaccataa agcactaaat ctctttcttc
    15181 ctcagtcaag gcctcaaaag gtgtatccat atccacccca aaacttgcca tagcttgctc
    15241 aagcatggtt gggtaataat tggaggaaat aggattccaa ggcgccaaag ctccctctct
    15301 caaagactta cttgggtcag gaacaaccaa gtccaaatcc acttccaact taatgcctag
    15361 cccatcacag gtcggacaag agccgaacgg cgcattaaaa gagaagagtc ttggttctaa
    15421 ttcaggaacc gtaaacccac agacaggaca ggaataatgc tcagaaaaga gcagttcatt
    15481 gccatccata gtgtcaatca taaggtagcc atctcccagc cttaaagctg cttcaaccga
    15541 atcaaataaa cggctacgaa taccatcctt attaaccaga cggtcaataa ccacctcgat
    15601 gttgtgcatc ttacttttgg acagctccgg cacttctgtc acgtcaaaaa tatccccgtc
    15661 aacccgcaca cggacataac catctttttg aattttctca aaaatggttt tgtgttgacc
    15721 ttttttccgg cgaacaatag gcgctaaaat ctgcatgcgt gtccgttcag ggagggctaa
    15781 gacctgctct acaatttgtt ctgcagaaga tgctgtaatg gccccatgcc cattaataca
    15841 gtaaggtgtg cccacacgcg catatagtaa acgtaaataa tcattgattt ccgtaaccgt
    15901 accaaccgtt gagcgggggt tcttactggt agttttttga tcaatggaaa tagcagggct
    15961 aagaccgtca atggaatcca catctggttt ttccatatta cccaagaact gtctagcata
    16021 agctgataaa ctttctacgt aacgacgttg cccttccgca taaatagtat caaaggctag
    16081 actagatttt cctgaccctg ataaccctgt cacgacaact aatttgtctc gtggaatttc
    16141 cacgtcaata ttttttaaat tgtgggctct tgccccatga attattattt tattttgcat
    16201 cttgaatgtg cctaaaggca ccctcctcat cgagctttct tatattataa caaaaaagaa
    16261 ccttaatatg gtatcatttg ctatcattgc ccaatcactt gataaatcaa cattttacaa
    16321 ccttacatta tctagtcaat tacactgatt tttaaaataa cgtagtaatt ttccgctggg
    16381 gagcatctga ttttggactt ggaaaagtct tttttatttg tctaattcca ccatcacttc
    16441 ttccactatc tcctcggtca caacttctcc attttttact ttctcatgtg gcaaaacata
    16501 atcaaaaatc tgtccgtttt tgcgcacaat cgctactgtg tctcctgtga tatacccatg
    16561 gtcgattgct tgcttaaatt cgtcgtatgt tagcatagat tacctcctta tcttattatt
    16621 cgtattttac aggaaataat atagttagat ttaaaactta tttaaaattt aataacatct
    16681 actttattac aaataaaacc acttcttatt aacgaatatt aaataaaaag aaattttagt
    16741 acaatattgg caacaaatta attttattaa ttttaagttt ttttaattca aagtggtata
    16801 cttaacctgt aaataattaa aggaggacat ggatatgtct aaaaagctta gaaactctct
    16861 cgccagaata gttgttgcag cattcgcatc atttgctgtg atggcgattc caacttatca
    16921 tcacagtaca gtcttagcta aaacagtctc agtaaatcag acttatggtg aatataaaga
    16981 ctattatact gtaattggtg agtcaaacat tgaccaatct gcttttccta agatatataa
    17041 aacaaccgag agagtctata aaggacaagg tacaagcgaa aaaagagtaa ccgtatctga
    17101 tgtagtttat aacccattag atggctataa acgttcaaca ggcgcttacg gcgttgtcac
    17161 aaaagatatg attgacatgt ctaaaggata ccgtgaaaaa tgggaaacaa atcccgaacc
    17221 aagcggttgg ttcagatttt ataatagagc cgataatgaa gaaattagtg agaaagaata
    17281 tgattcaaga cgtactaagt catacaaagt taccaacaat gtacctgtag tgttaacaac
    17341 attaaaaggt aaacaataca acagtcactt atttgtacgt agccatcttt ttgccgatag
    17401 ccttggagga aagtccatac ggaaaaacgc tattactgga actcaaatgc aaaatgtagg
    17461 aacacgtaaa ggtgggatgc aatatattga gaaaaaagtt ttaagccata tcactaagaa
    17521 tcctgatgtt tatgtattct acagcgccat ccctgaatat caggggactg agttattagc
    17581 aagatcagtt ttagtatctg ctttatcttc cgatggagta attaatgaaa ctgttcgtgt
    17641 tttcaacacc gctgatggat ttaatattaa ttatgaaaag ggcggactct taacagaaag
    17701 ccctgtaagt gaaattgata atattgaaga ttctacaaca gacgaaatag aaaattctgt
    17761 ggatgattca gaagaaatag tttataacga tactactacc gaagaagaaa actaagattt
    17821 caaaaaattt ttagacacaa aaaaaccgcc cagaaataat ctgagcggtt ggttttttat
    17881 ttcggctaat tcttactatg gtaattggtt catgattgtg cgagggtgtc aacgaagata
    17941 atcggcatcg gtaatgcttt ttaatctgtc ggtatagttt ggaaatgtag agaatttgaa
    18001 acgtttagga tctgttaatt tatgttgatc actattagct acagcttgat tataatctat
    18061 gatagccttt atcaacttgt taacaataag tatatcatta tcaacaatct taaaataatc
    18121 aacctgctct cgtaatttga acgttctgtt taaaatatat tttggatctt caactactag
    18181 agctttgcca atatcaagcc cctctacgca tccgtatttt ttagagtctc ttaatttata
    18241 ttttagttga tattttttag gaatattact tctcaaaggg attgcaaaag ttagattttg
    18301 gatacttacc aacacaatag caaaacctcg gcctttgtta tatagcttct tctctttaaa
    18361 atcatagtct aaagcttgta ccaaatcgaa atctcgacac atgtttaaat caatctgacc
    18421 gaatactaat tttttactct tcattatttg ccttctaaaa ataaaaaatc ctaccaactg
    18481 ggtattggta ggacgcattg ccaacgggat acttctgtta acgagcgcgt ccctccgccc
    18541 cctcattgcc aaaatggcga gagtttaacg acatggaaag gtcggaacat agagataatg
    18601 cttttactgt ttatgttcat ttacattatt acataatatg ttgcattcgt caaatataga
    18661 atctttaaga cacaaaaacc gccctcgatt aagagagcgg ttttgtctta tctaaaggag
    18721 ctttacctcc ataatttttt gctaccgaca ttgatgtcgg ttacactaat ttaatttacc
    18781 ccaaatactg atgcggttcc cgtctttatc agtctgtcca atagctaggt agtcacgcat
    18841 acctaagcct ccaacatagc taatccaata atagccatta gcgtagccct cgctatcaaa
    18901 gctgacagta tcaccttgct tgtagatacc tacaacctcg ctggctagac ttggccagcg
    18961 tctgatattg atctctgcaa catcaagggt aaaggtgcct gtttttgctg tctctacgat
    19021 agtgtcagaa gtttgcggct cggtgctgac tggttgcgtg actgtatccc cttgatatgg
    19081 tgggtaaaac catccaacaa cgccagtaaa gtcacgagta ttaaaacgag ctggtgcgcc
    19141 gacatataag gcgtctgcat tgccatcaat gttttgttca acagtccgca tagtgtagcc
    19201 gtcgctatcc tcaat
//
LOCUS       DAUDYX010000041        18682 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.041, whole genome shotgun sequence.
ACCESSION   DAUDYX010000041 DAUDYX010000000
VERSION     DAUDYX010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18682
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.041"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            129..1730
                     /locus_tag="VHD02_001400"
     CDS             129..1730
                     /locus_tag="VHD02_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ5498003.1"
                     /translation="MYVSKLFTSQQWRELKLISYLTENSNAIGVKDKELSKALNISML
                     TLQSCLTNMQFMKEVGGITYKDGYINIWYHQCCGLQEVYQKALRESPSLKLLELLFFR
                     DFSSLEELAEELFVSLSTLKRLIKKTNTYLSHTFAISIVTSPVQVSGNERQIRLFYLK
                     YFSEAYKISEWPFGDILNLKNCERLLSLLIKEVDVKVHFTLFQHLKILSGVNLIRYYK
                     GYSCSYNNKKTSHRFSQLIQHSSEIQDLSRLFYLKFGLHLDEYTIAEMFSNHLNDKLE
                     IGCAFEIINQDPTSGGRQVTNWIHLLDEMEIKLNLSITNKYEVAVTLHNASVLNEEDI
                     TANYLLFDYKKSYLNFYQKEHPRIYEAFVTSVEKLMQADNAQVSKELINQLTYCFFIT
                     WENSFLKVNQKDEKVRLLVIERSYNSVGNFLKKYIGEFFSITNFDELDCLTIDLVEIE
                     KQYDVIVTDVMVGKSEELEIFFFYKMIPEAIIDRLNEFLNVSFTDNNVMVKNLEAPSP
                     SKSHSDKEVQKPEKPDNSVNQATSS"
     gene            1918..3177
                     /locus_tag="VHD02_001401"
     CDS             1918..3177
                     /locus_tag="VHD02_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407887.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEQ5498004.1"
                     /translation="MSKRNPNKHYSLRKLKTGTASVAVALTVLGTGLATGQTIKADTS
                     EQEHPNITKIKEQLLRNKLVPATQWYQAYTKSEELSRQVEKLQTLVQSKTSNYEKIKS
                     QLQELEQSKKALEDTFYSTLRDHSSLFKEQDKEIKNMRAQLEFKNKQISDLTNENAQL
                     KEAIEGYVQTIQNASREIAAKQQELAAAKSQLEAKNAEIEALKQQDASKTEEIAKLQS
                     EAATLENLLGSAKRELTDLQAKLDTATAEKAKLESQVTTLENLLGSAKRELTDLQAKL
                     DAANAEKEKLQSQAAALEKQLEATKKELADLQAKLAATNQEKEKLEAEAKALKEQLAK
                     QAEELAKLKADKASGAQKPDTKPGNKEVPTRPSQTRTNTNKAPMAQTKRQLPSTGEET
                     TNPFFTAAALTVIASAGILALKRKEES"
     gene            3402..4499
                     /locus_tag="VHD02_001402"
     CDS             3402..4499
                     /locus_tag="VHD02_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561044.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEQ5498005.1"
                     /translation="MARKDTNKQYSLRKLKTGTASVAVAVAVLGAGFTNQTTVKADSS
                     SRDITEAGVSKFWKSKFDAEQNRANELEKKLSGYEKDYKTLEQEYENLEETLQKFGND
                     YERLENKNNEYANQLGKNQEEREKLELEYLRKSDKEYKEHQQYRQEQEERQKNLEELE
                     RQNKREIDKRYQEQLQKQQQLETEKQISEASRKSLSRDLEASRAAKKGLEAEHQKLKE
                     EKQISDASRKSLSRDLEASREAKKKVEADLAEANSKLQALEKLNKELEEGKKLSEKEK
                     AELQARLEAEAKALKEQLAKQAEELAKLKGNQTPNAKVAPQANRSRSAMTQQKRTLPS
                     TGEAANPFFTAAAATVMVSAGMLALKRKEEN"
     gene            4834..8280
                     /gene="scpA/B"
                     /locus_tag="VHD02_001403"
     CDS             4834..8280
                     /gene="scpA/B"
                     /locus_tag="VHD02_001403"
                     /EC_number="3.4.21.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C5a peptidase ScpA/B"
                     /protein_id="HEQ5498006.1"
                     /translation="MRKKQKLPFDKLAIALMSTSILLNAQSDIKANTVTEDTPATEQA
                     VETPQPTAVSEEAPSSSKETNPPQIPDDAEETVADKANDPAPQAPAKTADTPATSKAT
                     IRDLNDPSQVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKEDLEKA
                     KKEHGITYGEWVNDKVAYYHDYSKDGKTAVDQEHGTHVSGILSGNAPSETKEPYRLEG
                     AMPEAQLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDET
                     KKAFDYAKSKGVSIVTSAGNDSSFGGKTRLPLADHPDYGVVGTPAAADSTLTVASYSP
                     DKQLTETATVKTDDHQAKEMPVLSTNRFEPNKAYDYAYANRGTKEDDFKDVKGKIALI
                     ERGDIDFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRKDGLLLKDN
                     PQKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAK
                     LSGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYF
                     SPRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELY
                     YQATVQTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLSQMK
                     NGYFLEGFVRFKQDPKKEELMSIPYIGFRGDFSNLSALEKPIYDSKDGSSYYHEANSD
                     AKDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGT
                     FAKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGN
                     VVWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPIS
                     SGAKEQHTDFDVIVDNTTPEVATSATFSTEDRRLTLASKPKTSQPVYRERIAYTYMDE
                     DLPTTEYISPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLE
                     GHSNKPEQDGSDQAPDKKPETKPEQDGSGQTPDKKPEAKPEKDSSGQTPGKTPQKGQP
                     SRTLEKRSSKRALATKASTKDQLPTTNDKDTNRLHLLKLVMTTFFLGLVAHIFKTKRT
                     ED"
     gene            8377..9558
                     /locus_tag="VHD02_001404"
     CDS             8377..9558
                     /locus_tag="VHD02_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HEQ5498007.1"
                     /translation="MFRAENNKHSRYSIRKLSVGVTSIAIASLFLGKVAYAVDTVQSI
                     SSTQENTANTSRKWYDIDDRGFIPVGISLETAKEEFKKEVEESHLPQEQKETYKQKID
                     AETDKDELLFTYHSEYMTAVKNLPTSTEPVEASVQETQASVSDSMVTDDSTSVTTDSP
                     KESPVAPASSPESEDSSVASSEETSSPETPVAPETPEEPAAPSPSPESEEPSVAAPSE
                     ETPTPSTPEEPLAPVQPAESEEPSVAASSEETPTPSTPEEPAAPSPSSESEESSVAAT
                     TSPSPSTPAESETQTPPAVTKDSDKPSSAAEKPAASSLVSEQTVQQPTSKRSSDKKEE
                     QEQSYSPNRSLSRQVMAHESGKYLPSTGEKAQPLFIATMTLMSLFGSLLVTKRQKETK
                     K"
     gene            9810..10730
                     /gene="lmb"
                     /locus_tag="VHD02_001405"
     CDS             9810..10730
                     /gene="lmb"
                     /locus_tag="VHD02_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEQ5498008.1"
                     /translation="MKKGFFLMAMAVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVDVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGRLDPKHKDSYTKNAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
     gene            10743..13214
                     /locus_tag="VHD02_001406"
     CDS             10743..13214
                     /locus_tag="VHD02_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEQ5498009.1"
                     /translation="MKKTYGYIGSVVAILLATHIGSYQLGKHHMGSATKDNQIAYIDD
                     SKGKAKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYRFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPGRRKAPIPDVTPNP
                     GQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEEDGL
                     IFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEIELADRYLAGQTEDNDSGSEHSK
                     PSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHFHY
                     IGFGELEQYELDEVANWVKAKGQADELAAALDQEQGKEKPLFDTKKVSRKVTKDGKVG
                     YMMPKDGKDYFYARDQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSSLP
                     MHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRDQIATIKYVMQHPEVRPDVWSKPGHE
                     ESGSVIPNVTPFDKRAGMPNWQIIHSAEEVQKALAEGRFATPDGYIFDPRDVLAKETF
                     VWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPKEKQQAD
                     KSNENQQPSEASKEEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIFQP
                     EGVQFYNKNGELVTYDIKTLQQINP"
     gene            13383..13583
                     /locus_tag="VHD02_001407"
     CDS             13383..13583
                     /locus_tag="VHD02_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEQ5498010.1"
                     /translation="MSEEKLKSKIEQASGGLKEGAGKLTGDKELEAKGFVEKTIAKGK
                     ELADDAKEAVEGAVDAVKEKLK"
     gene            complement(13666..14292)
                     /locus_tag="VHD02_001408"
     CDS             complement(13666..14292)
                     /locus_tag="VHD02_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5498011.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEIDLEVAPGQILGIFGQSGCGKTS
                     LSRVLAGFLQPKSGEVLVDGSHLPNKAFRPVQLIQQHPEQTMNPLWPMKKSLEEAYYP
                     SQDLRDAFGIQEKWLKRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIVISHDFAMLEKLCDACYMIEENRTQLFKHT"
     gene            complement(14276..15079)
                     /locus_tag="VHD02_001409"
     CDS             complement(14276..15079)
                     /locus_tag="VHD02_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5498012.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEVKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNAAVTGDMIYRGQSLTSKRIKQLRGKEMTLIPQSVN
                     YLDPSMKVKHQVRLGISENAKATQEGLFQQFGLKESDGDLYPFQLSGGMLRRVLFTTC
                     ISDTVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGDGEQLQTEFARCLWRTLPQQDFLKGVTHDLRG"
     gene            complement(15091..15912)
                     /locus_tag="VHD02_001410"
     CDS             complement(15091..15912)
                     /locus_tag="VHD02_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ5498013.1"
                     /translation="MILKRRTMVLWQLGIAISLILSILALNLYFYRTPLETNAALRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVFLATVFGVLAGLGNSLID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYDLKN
                     KAFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVIFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            complement(15909..16886)
                     /locus_tag="VHD02_001411"
     CDS             complement(15909..16886)
                     /locus_tag="VHD02_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ5498014.1"
                     /translation="MKRTTIIIIWKIIRCITLIFGVSVLTFVLLKQSPVDPVMASVNY
                     DTSLTPAQYKAIAHHYGLDKPALVQYFIWLKNVIQGHLGTSLVYRQPVSDIIRSRAGA
                     SFILMGLSWILSGLIGFILGTLSAFHQGKLLDRVVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRVKHLMLPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETQRQIFKNHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            complement(16999..18627)
                     /locus_tag="VHD02_001412"
     CDS             complement(16999..18627)
                     /locus_tag="VHD02_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEQ5498015.1"
                     /translation="MSKYLKYFSIITLFLTGLILVACQQQKPQTKERQRKQRPKDEIV
                     VSMGAKLPHEFDPKDRYGVHNEGNITHSTLLKRSPELDIKGELAKTYHLSEDGLTWSF
                     DLHDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQVNIHLTEAHS
                     TFTAQLTEIPIVPKKHYNGKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFK
                     KWTWVLLDENTALAALESGDVDMIYATPELASKKVKGTRLLDIPSNDVRGLSLPYVKK
                     GVITDSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKPAYSIIDKTPFWN
                     PKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGDLDAAFDLYYPTNDQLRANLAVEVA
                     EQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHHPSLAGKGWTNIT
                     FYNNPTVTKYLDKAMTSSDLDKANEYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIG
                     DKRINVGKQGVHSHGHDWSLLTNIAEWTWDESTK"
BASE COUNT         6492 a         3717 c         3648 g         4825 t
ORIGIN      
        1 tttttggatt ttaatgaaag aatttgaaag gcattttatg gtatactttt tcttgatata
       61 ggtcgtactg acttaacgaa atacctcacg aaataacaag aaaattaaaa agaagggtat
      121 acaaggtaat gtatgtaagt aagttgttta caagtcaaca gtggagagaa ctaaaattaa
      181 tctcatattt aacagaaaac tccaatgcta tcggagttaa agataaggag cttagcaagg
      241 ctctcaatat ttccatgtta acattacagt catgcctcac taatatgcag ttcatgaaag
      301 aagtaggtgg aattacctac aaagacggct atattaacat ttggtatcac cagtgttgtg
      361 ggctacaaga ggtctatcaa aaagctcttc gagaatcgcc ttctttgaaa ttactagagc
      421 ttttgttttt cagagacttt agttccttag aagagttagc tgaagagctg tttgtcagct
      481 tatctaccct caaacgcctc atcaaaaaaa ccaacactta cttgtctcat acctttgcta
      541 ttagtatcgt gacaagtcca gtgcaggtat caggaaatga gaggcaaatc cgcctgtttt
      601 acctcaagta tttttcagaa gcctataaaa tctcagaatg gccatttggg gacattttaa
      661 acctgaaaaa ctgtgaacgc ctattgagct tattgatcaa agaagtagat gtcaaagttc
      721 attttacact atttcagcac ttaaaaatct taagtggtgt caacttgatc cgttactaca
      781 aggggtattc ttgcagttat aacaataaaa aaaccagtca ccgtttttct caactcatcc
      841 aacattcctc agaaatccag gatctttctc gcttattcta cctcaaattt gggttacatc
      901 tcgacgagta taccatagct gaaatgtttt ctaaccatct taacgacaag ctagaaattg
      961 gctgtgcttt tgagattatc aatcaagacc cgacatcagg aggcagacaa gtaaccaact
     1021 ggatccatct attagatgag atggaaatca agctcaatct cagcatcacc aataaatacg
     1081 aagttgctgt aaccctccat aacgcttcgg tgttaaacga agaagacatt acagccaatt
     1141 atttgctgtt tgattataaa aagagttacc tgaactttta ccaaaaagaa caccctcgta
     1201 tctatgaggc ctttgtgaca agtgtcgaga agctgatgca ggcagataat gctcaagtta
     1261 gcaaagaatt gatcaatcag ctcacttatt gtttctttat tacctgggaa aatagtttct
     1321 taaaagtaaa tcaaaaagat gaaaaagtac gacttctagt gatagagaga agttacaata
     1381 gtgtcggtaa ttttttgaaa aagtacatcg gtgagttttt cagcattact aattttgatg
     1441 agttagattg tttgacaatt gatctagtag agattgaaaa acagtacgat gttatcgtga
     1501 cagatgttat ggtgggtaaa agcgaagagt tagagatttt ctttttctac aagatgatcc
     1561 cagaagctat tattgataga ttaaacgaat ttttgaacgt tagtttcaca gataacaacg
     1621 ttatggtcaa aaacctcgaa gccccttccc cttcaaaatc tcatagtgac aaagaggtac
     1681 aaaagccaga aaagccagac aattcagtta atcaagcaac atcatcatag gatttcagac
     1741 gtcatggtaa gaaaaaaaag aatcatttac agcccataga ggtaaggtca aaagctgaaa
     1801 gcagctcaaa aaaactgacc tttacctttt ggctttttta ttttagaata attttattgg
     1861 agagatgctt aataatttaa gcacaattct tagaaattga gaaataagga gtaaacaatg
     1921 tctaaaagaa atccaaacaa acactactca ctgagaaagt taaaaacagg tactgcatca
     1981 gtagcagtag ctttgacagt tttggggact gggttagcaa ctggacaaac aataaaagct
     2041 gataccagtg aacaagaaca cccgaacatt acaaagatca aagaacagct tttacgcaat
     2101 aagttggtcc ctgctacaca atggtaccaa gcctatacta aaagcgagga acttagtcgt
     2161 caggtcgaaa aacttcagac cttggtccaa tcaaagacct caaattatga aaagataaag
     2221 tcacagttac aagagttgga gcaatctaaa aaggctttgg aggacacttt ttacagtaca
     2281 cttagagatc actcaagtct tttcaaagaa caagataaag aaatcaaaaa tatgagggcc
     2341 cagcttgagt ttaaaaacaa gcaaatctca gatttaacca acgaaaacgc acagttaaaa
     2401 gaagccattg aaggttatgt gcaaactatc caaaacgcta gtcgtgaaat cgcagcaaaa
     2461 caacaagaac ttgcagctgc aaaaagccag ttagaggcaa aaaatgctga gattgaggca
     2521 ttgaaacaac aagatgcctc taagactgaa gaaattgcta aattgcaatc agaagcagca
     2581 actttagaaa accttctagg ttcagccaag cgtgagttga ctgacttgca agctaagcta
     2641 gatacagcaa ctgctgaaaa agcaaaacta gaatcacaag taacaacctt agaaaacctt
     2701 ctaggttcag ctaagcgtga attgactgat ctgcaagcta agctagatgc agctaacgct
     2761 gaaaaagaaa agctccaatc acaagcagca gccctagaaa aacaactaga agcaactaaa
     2821 aaagagttag ctgatttaca ggctaaatta gcagcaacca accaagaaaa agaaaagtta
     2881 gaagctgaag caaaagctct taaagagcaa ttagcaaaac aagctgaaga gcttgctaag
     2941 ctaaaagcag ataaagcttc aggggctcaa aaaccagata ctaaacctgg caataaagag
     3001 gttccaacaa gaccatcaca aacaagaaca aacactaata aagctcctat ggcgcaaaca
     3061 aagagacaat taccgtcaac aggcgaagaa acaaccaacc cattcttcac tgcagcagca
     3121 ttgacagtga tcgcatctgc aggcatactt gccctaaaac gcaaagaaga aagctaagcc
     3181 caactcacaa tatcttttct agcccaagaa aaaaacaaaa aaagaggaag accattcctc
     3241 tttttttgaa cggttgaaaa gcaaaaaggt caaaaaggca ctaaagtctc aaaaacctgg
     3301 tctttacctt ttaccgctta ttctttagaa tagaattatt agagagaagt cttagaaaaa
     3361 tgaggctaat tccctaaaga tgaaaaaata aggagcaaat aatggctaga aaagatacga
     3421 ataaacagta ttcgcttaga aaattaaaaa caggtacagc atcagtagcg gtcgctgtgg
     3481 ctgttttagg agcaggcttt acaaaccaaa caacagttaa ggcggactct tctagtagag
     3541 atatcactga ggcaggtgta tctaagtttt ggaagagtaa gtttgatgca gagcagaata
     3601 gagcaaacga gcttgaaaaa aaacttagtg gatacgaaaa agattataaa acattagaac
     3661 aagagtatga aaatctagaa gaaactttac aaaaattcgg aaatgattat gaaagattag
     3721 aaaacaaaaa taatgagtac gcaaatcaac ttggtaaaaa tcaagaagaa cgcgaaaaat
     3781 tagagcttga atacctcaga aaatcagata aagagtataa agagcatcaa caatatcgac
     3841 aagaacaaga agaacgtcaa aaaaatctag aggaacttga acgtcaaaat aaacgagaaa
     3901 tagacaaacg ctatcaagaa caactccaaa aacaacaaca attagaaaca gaaaagcaaa
     3961 tctcagaagc tagtcgtaag agcctaagcc gtgacctcga agcttctcgt gcagctaaaa
     4021 aaggccttga agctgagcac caaaaactca aagaggaaaa acaaatctca gacgcaagcc
     4081 gtaagagcct aagccgtgac cttgaagcgt ctcgcgaagc taagaaaaaa gtagaagcag
     4141 acttagccga agcaaatagc aaacttcaag cccttgaaaa actaaacaaa gagcttgaag
     4201 aaggtaagaa attatcagaa aaagaaaaag ctgagttaca agcaagacta gaagcagaag
     4261 caaaagctct taaagagcaa ttggctaaac aagctgaaga acttgccaaa ctaaaaggca
     4321 accaaacacc aaacgctaaa gtagccccac aagctaaccg ttcaagatca gcaatgacgc
     4381 aacaaaagag aacgttaccg tcaacaggcg aagcagctaa cccattcttt acagcagcag
     4441 ctgcaacagt gatggtatct gcaggtatgc ttgctctaaa acgcaaagaa gaaaactaag
     4501 cctttaaaac ttggtttttg taacgatgca atagacaaaa gcaagcaagg ccaaaaactg
     4561 agaaagtcct aaaaagctgg cctttacccc tcaaaattaa tgttttataa taaagatgtt
     4621 agtaatataa ttgataaatg agatacattt aatcattatg gcaaaagcaa gaaaaatagc
     4681 tgtatcatat gcaaataacc cctgtttgct ctttaaaaaa gacgttatcc ttatttctcc
     4741 acgcacagat ggacagctag gagagaatcg tttgattctc tcttttctta atggtcataa
     4801 agacaaagtc tctcatcatg aaaggacgac acattgcgta aaaaacaaaa attaccattt
     4861 gataaacttg ccattgcgct catgtctacg agcatcttgc tcaatgcaca atcagacatt
     4921 aaagcaaata ctgtgacaga agacactcct gctaccgaac aagccgtaga aactccacaa
     4981 ccaacagcgg tttctgagga agcaccatca tcatcaaagg aaactaaccc tccacaaatt
     5041 cctgatgacg cagaagaaac agtagcagat aaggctaatg atccagcccc tcaagctcct
     5101 gctaaaactg ctgatacacc agcaacctca aaagcgacta ttagggattt gaacgaccct
     5161 tctcaggtca aaaccctgca ggaaaaagca ggcaagggag ctgggactgt tgttgcagtg
     5221 attgatgctg gttttgataa aaatcatgaa gcgtggcgtt taacagacaa aaccaaagca
     5281 cgttaccaat caaaagaaga tcttgaaaaa gctaaaaaag agcacggtat tacctacggc
     5341 gagtgggtca atgataaggt tgcttattac cacgactata gcaaagatgg taaaaccgct
     5401 gtcgatcaag agcacggcac acacgtgtca gggatcttgt cagggaatgc tccatctgaa
     5461 acgaaagaac cttaccgcct agaaggtgcg atgcctgagg ctcaattgct tttgatgcgt
     5521 gtcgaaattg taaatggact agcagactat gctcgtaact acgctcaagc tatcagagat
     5581 gctgtcaact tgggagctaa ggtgattaat atgagctttg gtaatgctgc actagcttac
     5641 gccaaccttc cagacgaaac caaaaaagcc tttgactatg ccaaatcaaa aggtgttagc
     5701 attgtgacct cagctggtaa tgatagtagc tttgggggca agacccgtct acctctagca
     5761 gatcatcctg attatggggt ggttgggaca cctgcagcgg cagactcaac attgacagtt
     5821 gcttcttaca gcccagataa acagctcact gaaactgcta cggtcaaaac agacgatcat
     5881 caagctaaag aaatgcctgt tctttcaaca aaccgttttg agccaaacaa ggcttacgac
     5941 tatgcttatg ctaatcgtgg gacgaaagag gatgatttta aggatgtcaa aggcaaaatt
     6001 gcccttattg aacgtggcga tattgatttc aaagataaga ttgcaaacgc taaaaaagct
     6061 ggtgctgtag gggtcttgat ctatgacaat caagacaagg gcttcccgat tgaattgcca
     6121 aatgttgacc agatgcctgc ggcctttatc agtcgaaaag atggtctctt attaaaagac
     6181 aatccccaaa aaaccatcac cttcaatgcg acacctaagg tattgccaac agcaagtggc
     6241 accaaactaa gccgcttctc gagctggggt ctgacagctg acggcaatat taagccagat
     6301 attgcagcac ccggccaaga tattttgtca tcagtggcta acaacaagta tgccaaactt
     6361 tctggaacta gtatgtctgc gccattagta gcgggtatca tgggactgtt gcaaaagcaa
     6421 tatgagacac agtatcctga tatgacacca tcagagcgtc ttgatttagc taaaaaagta
     6481 ttgatgagct cagcaactgc cttatatgat gaagatgaaa aagcttattt ttctcctcgc
     6541 caacaaggag caggagcagt cgatgctaaa aaagcttcag cagcaacgat gtatgtgaca
     6601 gataaggaca atacctcaag caaggttcac ctgaacaatg tttctgataa atttgaagta
     6661 acagtaacag ttcacaacaa atctgataaa cctcaagagt tgtattacca agcaactgtt
     6721 caaacagata aagtagatgg aaaacacttt gccttggctc ctaaagcatt gtatgagaca
     6781 tcatggcaaa aaatcacaat tccagccaat agcagcaaac aagtcaccgt tccaatcgat
     6841 gctagtcgat ttagcaagga cttgctttcc caaatgaaaa atggctattt cttagaaggt
     6901 tttgttcgtt tcaaacaaga tcctaaaaaa gaagagctta tgagcattcc atatattggt
     6961 ttccgaggtg attttagcaa tctgtcagcc ttagaaaaac caatctatga tagcaaagac
     7021 ggtagcagct actatcatga agcaaatagt gatgccaaag accaattaga tggtgacgga
     7081 ttacagtttt acgctctaaa aaataacttt acagcactta ccacagagtc taacccgtgg
     7141 acgattatta aagctgtcaa agaaggggtt gaaaacatag aggatatcga atcttcagag
     7201 atcacagaaa ccatttttgc aggtactttt gcaaaacaag acgatgatag ccactactat
     7261 atccaccgtc acgctaatgg caagccatat gctgcgatct ctccaaatgg ggacggtaac
     7321 agagattatg tccaattcca aggtacgttc ttgcgtaatg ctaaaaacct tgtggctgaa
     7381 gtcttggaca aagaaggaaa tgttgtttgg acaagtgagg taaccgagca agttgttaaa
     7441 aactacaaca atgacttggc aagcacactt ggttcaaccc gttttgaaaa aacgcgttgg
     7501 gacggtaaag ataaagacgg caaagttgtt gctaacggaa cctacaccta tcgtgtccgc
     7561 tacactccga ttagctcagg tgcaaaagaa caacacactg attttgatgt gattgtagac
     7621 aatacgacac ctgaagtcgc aacatcggca acattctcaa cagaagatcg tcgtttgaca
     7681 cttgcatcta aaccaaaaac cagccaaccg gtttaccgtg agcgtattgc ttacacttat
     7741 atggatgagg atctgccaac aacagagtat atttctccaa atgaagatgg tacctttact
     7801 cttcctgaag aggctgaaac aatggaaggc gctactgttc cattgaaaat gtcagacttt
     7861 acttatgttg ttgaagatat ggctggtaac atcacttata caccagtgac taagctattg
     7921 gaaggccact ctaataagcc agaacaagac ggttcagatc aagcaccaga caaaaaacca
     7981 gaaactaaac cagaacaaga cggttcaggt caaacaccag acaaaaaacc agaagctaaa
     8041 ccagaaaaag atagttcagg tcaaacacca ggtaaaactc ctcaaaaagg tcaaccttct
     8101 cgtactctag agaaacgatc ttctaagcgt gctttagcta caaaagcatc aacaaaagat
     8161 cagttaccaa cgactaatga caaggataca aatcgtttac atctccttaa gttagttatg
     8221 accactttct tcttgggatt agtagctcat atctttaaaa caaaacgcac tgaagattag
     8281 tacaactgat cagatggcta ctattgatga tgagttagat cagtcttctt ttcaaaagaa
     8341 ggcatagcag tataaaaata gatatgttag gacaatatgt ttagagcaga aaataacaaa
     8401 cacagccgct attccattcg caaactgagc gttggggtaa cgagtatagc aattgcgagt
     8461 ctctttttag gaaaggttgc ctatgccgta gataccgtgc agtcaatctc ttcgactcaa
     8521 gaaaacacag ccaatacatc ccgaaaatgg tatgatattg atgatagggg ctttattcct
     8581 gtaggtataa gcttagaaac tgccaaagag gaatttaaaa aagaagtaga agaatcacac
     8641 ctacctcaag aacaaaaaga aacgtataaa caaaaaattg atgctgaaac agacaaagat
     8701 gaactattat ttacgtatca tagtgagtat atgacagctg ttaagaatct tccaacgtct
     8761 actgagccag ttgaggcatc cgtgcaggag acacaggcat cagtttcaga ttcgatggtg
     8821 acagatgatt caacatcagt tacgactgat tctcctaagg aaagtccagt agccccagct
     8881 tcatcacctg agagtgagga ttcttcagta gcatcttctg aggaaacctc atctccagaa
     8941 actccagtgg ccccagaaac tcctgaagaa ccagcagctc catctccatc acctgagagt
     9001 gaagaacctt cagtagcagc tccttctgag gaaaccccaa ctccatcaac tccagaagaa
     9061 ccactagctc cagttcaacc agctgagagt gaggaacctt cagtagcagc ttcttctgag
     9121 gaaaccccaa ctccatcaac tccagaagaa ccagcagctc catctccatc atctgagagt
     9181 gaggaatctt cagtagcagc tacgacaagc ccgtctccat caactccagc tgaatcagag
     9241 actcagacgc caccagctgt tactaaagac tctgataagc catcttcagc agctgaaaaa
     9301 ccagcagcct cttcacttgt ttcagaacaa accgttcaac aaccaacttc aaagagatct
     9361 tctgataaga aagaagagca agaacagtct tactctccaa atcgctcatt gtcaagacag
     9421 gttatggccc atgagtcagg taagtacttg ccttcaacag gtgaaaaagc acagccactc
     9481 tttatagcta ctatgacttt gatgtctcta tttggcagtc ttttagtcac aaaacgccaa
     9541 aaagaaacta aaaaatagta ttacttaaac tgataagagc ttatttaata gagagaacca
     9601 atttggttct ctctattttt ttatatgtgc agcctcatca aaaagaagtt aggacaaata
     9661 gctgtcaaca attgcctttt gcttatccct tcagttgctg tgattttatc atacagtgat
     9721 aaaagatggt agttgccatt gacaaagtat tgataaagga gtaaaattaa ctggttaata
     9781 actggttaaa ttataattga ggaggtacta tgaaaaaagg tttttttctc atggctatgg
     9841 ctgtgagttt agtaatgata gcagggtgtg ataagtcagc aaaccccaaa cagcctacgc
     9901 aaggcatgtc agttgtaacc agcttttacc caatgtatgc gatgacaaaa gaagtatctg
     9961 gagacctcaa tgatgtgagg atgatccaat caggtgcagg cattcattcc tttgaaccgt
    10021 ctgtaaatga tgtggcagct atttatgacg cggatttgtt tgtttaccat tcacatacct
    10081 tagaagcttg ggcaagggat ctagacccta atttaaaaaa atcaaaggtt gatgtgtttg
    10141 aagcgtcaaa acctttgaca ctagatagag tcaaagggct agaagatatg gaagtcacac
    10201 aaggcattga ccctgcgaca ctttatgacc cacatacctg gacggatccc gttttagctg
    10261 gtgaggaagc tgttaatatc gctaaagagc taggacgttt ggatcctaaa cacaaagaca
    10321 gttacactaa aaatgctaag gctttcaaaa aagaagcaga gcaactaact gaagaataca
    10381 ctcaaaaatt taaaaaggtg cgctcaaaaa catttgtgac gcaacacacg gcattttctt
    10441 atctggctaa acgattcggc ttgaaacaac ttggtatctc gggtatttct ccagagcaag
    10501 agccctctcc tcgccaattg aaagaaattc aagactttgt caaagaatac aacgtcaaga
    10561 ctatttttgc agaagacaat gtcaatccca aaattgctca tgctattgcg aaatcaacag
    10621 gagctaaagt aaagacatta agtccacttg aagctgctcc aagcggaaac aagacatatc
    10681 tagaaaatct tagagcaaat ttggaagtgc tctatcaaca gttgaagtaa aggagattat
    10741 tagtgaagaa aacatatggt tatatcggct cagttgttgc cattttacta gctactcata
    10801 ttggaagtta ccaacttggt aagcatcata tgggttcagc aacaaaggac aatcaaattg
    10861 cctatattga tgatagcaaa ggtaaggcaa aagcccctaa aacaaacaaa acgatggatc
    10921 aaatcagtgc tgaagaaggc atctctgctg aacagatcgt agtcaaaatt actgaccaag
    10981 gctatgtgac ctcacatggt gaccattatc atttttacaa tgggaaagtt ccttatgatg
    11041 cgattattag tgaagagttg ttgatgacgg atcctaatta ccgttttaaa caatcagacg
    11101 ttatcaatga aatcttagac ggttacgtta ttaaagtcaa tggcaactat tatgtttacc
    11161 tcaagccagg tagcaagcgc aaaaacattc gaaccaaaca acaaattgct gagcaagtag
    11221 ccaaaggaac taaagaagct aaagaaaaag gtttagctca agtggcccat ctcagtaaag
    11281 aagaagttgc ggcagtcaat gaagcaaaaa gacaaggacg ctatactaca gacgatggct
    11341 atatttttag tccgacagat atcattgatg atttaggaga tgcttattta gtacctcatg
    11401 gtaatcacta tcattatatt cctaaaaaag atttgtctcc aagtgagcta gctgctgcac
    11461 aagcctactg gagtcaaaaa caaggtcgag gtgctagacc gtctgattac cgcccgacac
    11521 cagccccagg tcgtaggaaa gccccaattc ctgatgtgac gcctaaccct ggacaaggtc
    11581 atcagccaga taacggtggc tatcatccag cgcctcctag gccaaatgat gcgtcacaaa
    11641 acaaacacca aagagatgag tttaaaggaa aaacctttaa ggaactttta gatcaactac
    11701 accgtcttga tttgaaatac cgtcatgtgg aagaagatgg gttgattttt gaaccgactc
    11761 aagtgatcaa atcaaacgct tttgggtatg tggtgcctca tggagatcat tatcatatta
    11821 tcccaagaag tcagttatca cctcttgaaa tagaattagc agatcgatac ttagccggcc
    11881 aaactgagga caatgactca ggttcagagc actcaaaacc atcagataaa gaagtgacac
    11941 atacctttct tggtcatcgc atcaaagctt acggaaaagg cttagatggt aaaccatatg
    12001 atacgagtga tgcttatgtt tttagtaaag aatccattca ttcagtggat aaatcaggag
    12061 ttacagctaa acacggagat catttccact atataggatt tggagaactt gaacaatatg
    12121 agttggatga ggtcgctaac tgggtgaaag caaaaggtca agctgatgag cttgctgctg
    12181 ctttggatca ggaacaaggc aaagaaaaac cactctttga cactaaaaaa gtgagtcgca
    12241 aagtaacaaa agatggtaaa gtgggctata tgatgccaaa agatggcaag gactatttct
    12301 atgctcgtga tcaacttgat ttgactcaga ttgcctttgc cgaacaagaa ctaatgctta
    12361 aagataagaa acattaccgt tatgacattg ttgacacagg tattgagcca cgacttgctg
    12421 tagatgtgtc aagtctgccg atgcatgctg gtaatgctac ttacgatact ggaagttcgt
    12481 ttgttatccc tcatattgat catatccatg tcgttccgta ttcatggttg acgcgcgatc
    12541 agattgcaac aatcaagtat gtgatgcaac accccgaagt tcgtccggat gtatggtcta
    12601 agccagggca tgaagagtca ggttcggtca ttccaaatgt tacgcctttt gataaacgtg
    12661 ctggtatgcc aaactggcaa attatccatt ctgctgaaga agttcaaaaa gccctagcag
    12721 aaggtcgttt tgcaacacca gacggctata ttttcgatcc acgagatgtt ttggccaaag
    12781 aaacttttgt atggaaagat ggctccttta gcatcccaag agcagatggc agttcattga
    12841 gaaccattaa taaatctgat ctatcccaag ctgagtggca acaagctcaa gagttattgg
    12901 caaagaaaaa cgctggtgat gctactgata cggataaacc caaagaaaag caacaggcag
    12961 ataagagcaa tgaaaaccaa cagccaagtg aagccagtaa agaagaagaa aaagaatcag
    13021 atgactttat agacagttta ccagactatg gtctagatag agcaacccta gaagatcata
    13081 tcaatcaatt agcacaaaaa gctaatatcg atcctaagta tctcattttc caaccagaag
    13141 gtgtccaatt ttataataaa aatggtgaat tggtaactta tgatatcaag acacttcaac
    13201 aaataaaccc ttaaccaaaa gaagatctca ttgttaaagc actgctttgt caaagcaagt
    13261 tacggtgatt ttgaagtcat tttatgtaac gagtagtgat aaaagttgga taatagcggt
    13321 tttcttttgc aaagaaatgg tatccatgtt agaataataa aaaaaagagg aggattctaa
    13381 tcatgtcaga agaaaaatta aaatcaaaaa ttgagcaagc atctggtggc ctaaaagaag
    13441 gtgctggtaa gttgacaggt gataaagagc tagaagcaaa aggatttgtt gaaaaaacaa
    13501 ttgctaaagg caaagaactt gcagacgatg caaaagaagc ggttgaaggt gctgtagatg
    13561 ccgttaagga aaaattaaaa taattaaaaa aaaccagctg taagctggtt tttttaatgg
    13621 tgtgattttg ttaggaaaag actcaatagg taataaccgt aggtgttagg tatgcttgaa
    13681 cagttgagta cgattctctt caatcatata gcacgcgtca caaagttttt ctaacatagc
    13741 aaaatcatga ctaataacga tgagacctaa atttctatcc tttacaatct ctaacaggct
    13801 tttccataca ctagcttgtg taatgctatc caacatagta gtcatttcat ctgcaataag
    13861 gtatttcgtc tctggatgta atgaacgcac aatcgaaaag cgttgcaatt cccctcccga
    13921 gagttcacta gggcgacgct ttagccattt ttcttgaatg ccaaaagcat ctcgcaaatc
    13981 ttgacttgga tagtaggctt cttccaaact ttttttcata ggccataaag gattcatggt
    14041 ttgttcagga tgttgttgga taagttgtac aggtctaaat gctttgttag gcaaatgact
    14101 gccatcaact aatacttcac cagatttagg ctgcaaaaaa ccggcaagca ctctggataa
    14161 actcgttttt ccacacccac tttgtccaaa tatccctaag atttgaccag gtgctacctc
    14221 taaatcaatc tccttaaaaa gccattgatc ttttttatga taaaagccaa gctttttagc
    14281 ctctaaggtc atgagtaact cctttcaaaa agtcttgctg agggagtgtg cgccataaac
    14341 atctcgcaaa ttccgtttgt aattgctccc catctccgct aaaaaagcta gctggggctg
    14401 tttcaatagc ttttccctct ttaaaaatag ttatacgatc cgcaatttga ctagctgcta
    14461 caatatcatg agtgataaat atgacgctta ttcctttatc tgcaaaggag cgtagttgat
    14521 ctaaaaccat ttgcagagca tctggatgta atccaggggt aggctcatcc gcaataatca
    14581 aagaaaccgt atcactaata cacgttgtaa acaaaacacg tcggagcatt ccgccagaaa
    14641 gttggaaagg atacaagtca ccatcacttt cttttaaacc aaactgttga aacaatcctt
    14701 cttgagtagc cttagcattt tctgagatac ccaagcgcac ctgatgcttg actttcatag
    14761 atggatctaa ataattaacg gattgtggaa tcaacgtcat ttcttttcct cgcaactgtt
    14821 tgatgcgttt agaagttagt gattgaccac gataaatcat atctcctgtt acagctgcat
    14881 ttttaggaag aatatccata atagcatgtg ctaataaact cttgcctgaa ccactagcac
    14941 ctataatagc taataactca ccttttttaa cttctaaatt cagcgcttgg atcggtgtgg
    15001 attgaaatgg ttttaaaaat cttccgtatt gagtgaaggt gatggagagg tctttaatgc
    15061 ttaataatgt ttctgtcata tctctacctc ctaaaaatga tccgtctgag ggtaaaagag
    15121 tttctttaaa gattctccga tagtatcaaa ggcattgaca accaaaataa gataaaggcc
    15181 tggaaaaatc accaaccacc aattgccaag agagatatgc ttagctgcct ctgacaaaat
    15241 gataccaacc gaaggttgtt cggcagaaag gccaaatcct aagaaagtca tggatgcttc
    15301 atgcaagatg acgtgcggaa ataagaggat aaacccaatg aaaatttgag aagcaatcaa
    15361 aggcaggata tgatgcctca caatataata aggcgttttt cccatgcttt tagagagctg
    15421 gacaaaggct ttattcttta gatcatagac ttcattgcgg ataagccttg ctagagaggg
    15481 ccaatgggta acagctgttg caatgataac cccttgagcc cctttcccaa caacaaaaga
    15541 aatgagaatc ataaaaatca aatgaggcat accaataaac aaatcaacta accatgctat
    15601 tattttatca ataaggctat ttcctaaacc tgcaagcact ccaaaaacgg tcgcaagaaa
    15661 gactcccata agggcaccta ataagccgac ttgtaaagag aaataaagcc ctttaatcgt
    15721 tctgacaaac atatccctac ctaaaccatc tgtcccaaaa agatggttta aggaaggggc
    15781 gaggttgcgt aaagctgcat tggtttccaa aggcgtccta tagaaataaa ggtttaaggc
    15841 tagaatacta agaatgagag aaatggcgat acctagttgc cataaaacca tcgttcgacg
    15901 tttcaatatc atacttttct ccttaactgt ggattgatga tgctatttaa aatgtccgca
    15961 ataagattgc ccgcaaaaac aaataatgtc cctatcatca cgatagctag aagtagcggt
    16021 gtatcacttt taagtcctgc ttcagtgagg gtagacccta agccaggata tgagaaaact
    16081 tgctcagcaa gaacagatcc tccaaacaat tctccaaaat aggaaaaatg cagtgtaata
    16141 gctggtacga tagcatttct aagacaatga tttttaaaaa tttgccgctg tgtttcccca
    16201 cgcgctctgg caaataagac atattcacta gaaagcaccg acatcatttt agttctcgta
    16261 tgaagagtga cattggcaat gcctagaata cttagcgtga aaacaggtaa cataaggtgc
    16321 ttaacccgat cagctaacgt aatatcttga ctcaaagtcc caatcgggga agaaatacca
    16381 atcgggaacc accccagctg gacagaaaag attaataaaa aaatgaggcc aatccaaaac
    16441 gttggtactg atatctgaag gtaagaaaac cacctgacaa ctcggtcaag taatttccct
    16501 tgatggaaag cggataacgt tcctaagata aatccaataa gacccgataa gatccaagag
    16561 agtcccataa gtatgaaaga agcacctgcc cgtgatctaa taatatcact aacaggttgc
    16621 cgataaacga gcgaggtccc taaatgtccc tgtatcacat ttttcaacca aataaaatat
    16681 tggactagag ctggcttatc caagccatag tggtgagcaa tcgctttgta ctgagcaggg
    16741 gttagtgatg tgtcataatt gacacttgcc atgacgggat ctactggaga ttgttttaac
    16801 aaaacgaagg tcaaaacaga taccccaaaa ataagtgtga tacatctgat gattttccag
    16861 atgataataa tggtagtacg tttcacaatg aaatctcctt aatcgataag aaaaggagcc
    16921 gaggaaacga tcagcacagt tttgtgtatc ttgttttctc agactccaaa cttaaagtcg
    16981 ctgtattaac taaacgcgtt acttagttga ttcatcccaa gtccactcgg caatgttagt
    17041 caataatgac caatcatgac catgactgtg gacgccttgt ttacctacat tgatacgttt
    17101 atcaccaata taagtatggt taaggctcac caaccataca tttggcaaat ctccaagagt
    17161 agaagcacct gttttgccat cccactgcgc taacttccaa tattcgttag ctttgtcaag
    17221 gtcagaagat gtcattgctt tgtcaaggta cttagtcacg gtaggattgt tataaaacgt
    17281 aatattggtc caacctttac ctgctaggct tggatgatgt gattcataaa attgctgcgc
    17341 gtgatgacgt cctccggcat aaagtaaggc tgagtcatgt gacttcgttg ccatttcatc
    17401 ccagttacta gctttgagtt taatagtaat ccctagggct ttggcttgct ctgctacttc
    17461 aacggctaag ttcgctcgca attgatcatt agtagggtag tacagatcaa acgctgcatc
    17521 aaggtcacct tttttacggc taccgtctgc ttgttctttc catcccgctt ttgtcaacaa
    17581 ttgcttagct ttagctactt tattatcttt aatggctgtt tttggattcc aaaatggtgt
    17641 tttatcaata attgaataag ctggtttacc ataaccattt aaaaccgtat cgagaacttt
    17701 ttgtctattt aaaccaatag tcaaggcttt gcggattgct ggatcgctag tgacatcatt
    17761 tcctacagga taaccatcag gagaatcagt gatgacgcct tttttcacat aaggtaatga
    17821 taagccgcgc acatcatttg atggaatgtc aaggaggcga gtgcctttga ctttcttact
    17881 agcaagttct ggtgtcgcgt agatcatatc aacatcacca gattctaaag cagctagtgc
    17941 tgtgttttca tcaagtaaga cccaagtcca ctttttaaag tatggttttt tcccatgcca
    18001 ataagggtta cgaacaaaaa tagcttgttc tccagcctta tattctttta ccatgtaagg
    18061 tcctgaaccg ataggattgc tcttatactt accattgtaa tgttttttag ggacgattgg
    18121 gatttcagtc aactgtgctg taaatgtcga atgcgcctca gtcaaatgga tattgacctg
    18181 atttttccca actacttcaa cgttcttgat gaaggttaga tcccaagcct ttccatctgc
    18241 tttcaacata tcataagtaa acttaacatc atcagcagta acaggctcac catttgagaa
    18301 tttaaaatca tcatgcaagt caaacgacca agtcagccca tcttcagaga gatggtatgt
    18361 tttagcaagc tctcctttta tatctagttc aggagaacgt ttcaatagag tgctatgagt
    18421 gatattccct tcattgtgga ctccataacg gtcctttgga tcgaattcat gagggagctt
    18481 tgcccccata gaaacgacaa tttcgtcttt tggacgttgt ttgcgctgac gttcttttgt
    18541 ttggggcttt tgttgttgac atgcaactaa aataagccca gtcaaaaata acgtgataat
    18601 agagaagtat tttaggtatt ttgacacaat caaatctcct tatttctaat taataaataa
    18661 taatagtaac gactccatct ca
//
LOCUS       DAUDYX010000042        18645 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.042, whole genome shotgun sequence.
ACCESSION   DAUDYX010000042 DAUDYX010000000
VERSION     DAUDYX010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18645
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.042"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            366..806
                     /locus_tag="VHD02_001413"
     CDS             366..806
                     /locus_tag="VHD02_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498016.1"
                     /translation="MTKKNHGVFWGLLLGSAAASIAYLSLSSSKKDQLLKDSAKKIDD
                     LNAYLQDKSKQVLDAVSEKVQESKDAVEVYGGIAAETVEEKWQDTKDAVEVYGGIAAE
                     TVEESLGQAKEKVEGIGEATSQTIQSKMEKLTSDKTDADDDDEK"
     gene            complement(856..1767)
                     /locus_tag="VHD02_001414"
     CDS             complement(856..1767)
                     /locus_tag="VHD02_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEQ5498017.1"
                     /translation="MKTVRIFYNPNSGKKEDQLAGQVKDYFCQHGFSEDSVEVITPKD
                     ADQAFQLAKQAAKDKIDLVIPLGGDGTLNKIIGGIYEGGAHCLIGLVPSGTVNNFAKA
                     MHIPLQITEALDTILTGQIKQVDICKANQQYMISSLTLGLLADIAADVTAEEKRRFGP
                     LAFLKDSIRILKRNRSYAISLISHNHRIHLKTKFLLITMTNTIAGFPSFSPGAQADDG
                     YFQVYTMKKVSFFKFLWHINDFKQGDFSKAEEISHFQANTLSLLPQAKKQAILPRTRI
                     DGDKSDYLPIQLDIIPKAISIIVPTED"
     gene            complement(1884..2534)
                     /locus_tag="VHD02_001415"
     CDS             complement(1884..2534)
                     /locus_tag="VHD02_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEQ5498018.1"
                     /translation="MNQTFKQSLPLSFSEEVANSVTHAIGAFAMLILLPISASYAYQT
                     YDLKAAIGISIFVISLFLMFLSSTIYHSMAYGSVHKYILRIIDHSMIYIAIAGSYTPV
                     ALSLVSGWLGYIIIVLQWGITLFGILYKIFAKRINEKFSLMLYIVMGWLVVFILPVII
                     QKTSLAFGLLMLFGGLSYTIGAVFYAKKRPYFHMIWHLFILLASALQFIAITFFML"
     gene            complement(2531..2971)
                     /locus_tag="VHD02_001416"
     CDS             complement(2531..2971)
                     /locus_tag="VHD02_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEQ5498019.1"
                     /translation="MIFPSLPYWKDLPDLDLYLDQVLLYVNQCTDFSEVSDNKSLTAS
                     MINNYVKHGYVTKPIKKKYQKQQLARLIAISLFKTVFPIQDISRVLEELQAQADSESL
                     YNTFVTCWNQKAPIEEDIPPIVQVACQTVKDYHKTIYLLQEVTQ"
     gene            3190..4038
                     /gene="ylqF"
                     /locus_tag="VHD02_001417"
     CDS             3190..4038
                     /gene="ylqF"
                     /locus_tag="VHD02_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEQ5498020.1"
                     /translation="MATIQWFPGHMSKARRQVQENVKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADATRTKEWKAYYESQGIKTLAINSKEQSTVKKVTEAAK
                     ELMADKIQRLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFKTHY
                     PSRLTKRFKNIPLEEEAPEIIMTLTRQLGFKDDYDRFYTLFVKEVRDGKLGQYTLDQV
                     GDMDVD"
     gene            4028..4819
                     /locus_tag="VHD02_001418"
     CDS             4028..4819
                     /locus_tag="VHD02_001418"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEQ5498021.1"
                     /translation="MSTSIKAIKESLEAVTSLLDPLFQELATDTRSGVQKALKSRQKA
                     IQAELAEEERLEAMLSYEKALYKKGYQAIAGIDEVGRGPLAGPVVAACVILPKYCKIK
                     GLNDSKKIPKSKHETIYQAVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLE
                     SQLTQPDYLLIDAMTLDIAISQQSILKGDANSLSIAAASIVAKVTRDQMMANYDRIFP
                     GYGFAKNAGYGTKEHLQGLKAYGITPIHRKSFEPVKSMCCDSTNP"
     gene            4884..5720
                     /gene="dprA"
                     /locus_tag="VHD02_001419"
     CDS             4884..5720
                     /gene="dprA"
                     /locus_tag="VHD02_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEQ5498022.1"
                     /translation="MNHFELYKLKKAGLTNKNILNILDYQEHQEKSLSLRDMAVVSGC
                     KHPSHFIEAYKQLDIQNLKIEFKQFPSISILDKHYPMALKEIYNPPVLLFFQGNLDLL
                     EKPKLAIVGSRRSSDTGVKSVRKILKELGNRFVIVSGLARGIDTSAHLACLKNGGQTI
                     AIIGTGLDRFYPKENRELQTFLGKNHLVLTEYGPGEEALSYHFPERNRIIAGLSRGIL
                     VVEAKNRSGSLITCQIGIEEGRDIFAVPGNILDGKSEGCLQLIKEGATCVTSGMDILS
                     EY"
     gene            5827..7956
                     /gene="topA"
                     /locus_tag="VHD02_001420"
     CDS             5827..7956
                     /gene="topA"
                     /locus_tag="VHD02_001420"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEQ5498023.1"
                     /translation="MGTKTTTKPKTGTKKSTTKKKSIAKKNLVIVESPAKAKTIEKYL
                     GRSYKVVASVGHIRDLKKSSMSINFDNNYEPQYINIRGKGPLINSLKKEAKAAKKVYL
                     ASDPDREGEAISWHLSHILGLDPQDNNRVVFNEITKDAVKHAFVEPRQIDMDLVDSQQ
                     ARRVLDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENDIKAFVPKEYWSIDG
                     LFKKGTKKFQATFYGINGKKTKLDNNNDVKEVLAKLTNEDFLVSKVDKKERRRNAPLP
                     YTTSSLQQDAANKINFRTRKTMMVAQQLYEGIHLGENGTQGLITYMRTDSTRISPVAQ
                     NDAAQFIINRFGANYSKHGNRVKNTSGAQDAHEAIRPSSVNHTPDSIAKYLNKDQLKL
                     YTLIWNRFVASQMTAAVFDTVKVNLEQNGVIFVANGSQMKFDGYMAVYNDSDKNKMLP
                     EMEEGENVKKVSTSPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTLEVIQRRYY
                     VKLSAKRFEPTELGEIVNKLIVEFFPDIVDVAFTAEMEGKLDQVEIGEEEWQHVIDQF
                     YQPFVKELNKAESEIEKIQIKDEPAGFDCDVCGHPMVIKLGRFGKFYACSNFPECRNT
                     KAITKEIGVTCPVCHKGQVIERKTKKNRIFYGCDQYPDCEFISWDLPIGRACPKSGDY
                     LIEKKVRGGKQVMCSNETCDYKEEKIK"
     gene            complement(8031..8915)
                     /locus_tag="VHD02_001421"
                     /pseudo
     CDS             complement(8031..8915)
                     /locus_tag="VHD02_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            8928..9494
                     /locus_tag="VHD02_001422"
                     /pseudo
     CDS             8928..9494
                     /locus_tag="VHD02_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
     gene            9509..10501
                     /locus_tag="VHD02_001423"
     CDS             9509..10501
                     /locus_tag="VHD02_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEQ5498024.1"
                     /translation="MKLKLYNVRGEEAVLAKKWADDNGIEISLTESSLTPETVKEAEG
                     FDGIANAQIGPLDDAIYPLLKEMGIKQIAQRSAGVDMYNLDLATENDIIITNVPSYSP
                     ESIAEFTVTIALNLIRHVELIRENVKKQNFTWRLPIRGRVLGDMTVAIIGTGRIGLAT
                     AKIFKGFGCKVVGYDIYQSDAAKAVLDYKESVEEAIKDADLVSLHMPPTAENTHLFNA
                     DLFKSFKKGAILMNMARGALIETQDLLDALDAGLLSGAGIDTYEFEGPYIPKNFEGQE
                     ITDSLFKALINHPKVIYTPHAAYYTDEAVKNLVEGALNATVEIIKTGTTTTRVN"
     gene            10606..11271
                     /locus_tag="VHD02_001424"
     CDS             10606..11271
                     /locus_tag="VHD02_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEQ5498025.1"
                     /translation="MTYIALIGDIIQSKQLTDRSKVQKTLAAYLDDLNKTFAPYIISK
                     LSLTLGDEFQGLFQVDTPIFHLIDLINHHMDIPIRFGVGVGSILTDINPDISIGADGP
                     AYWHAREAIRYIHQKNDYGNTTLALRTGHHNQDDVLNSLLAAGDAIKANWRASQWEIF
                     DTLLDLGIYEEYFDQQRLGKQLSLSSSALSKRLKSSHVKIYLRTRQSALNCLKQIKEE
                     TDD"
     gene            11264..11980
                     /locus_tag="VHD02_001425"
     CDS             11264..11980
                     /locus_tag="VHD02_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEQ5498026.1"
                     /translation="MINGVSHYLAQTPTLTLFLICHVLSDYQLQSQQVADLKEKHLTY
                     LGYHLIGVSIPLICLTLIIPQAWLMSLLVMISHALIDWLKPKMANSLKWKREWIFLLD
                     QCLHIAISSFAGLRLAGVTLPNWLPISILMTVLFILVITKPTNIVFKLFFSKYQPDQG
                     EKMDTIIGAGATIGILERIVIGLCMIMGQFASIGLVFTAKSIARYNKISESPAFAEYY
                     LIGSLFSILSVFIAAWICFF"
     gene            12143..13489
                     /gene="trmFO"
                     /locus_tag="VHD02_001426"
     CDS             12143..13489
                     /gene="trmFO"
                     /locus_tag="VHD02_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEQ5498027.1"
                     /translation="MSQSTATYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKATP
                     QHKTTNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEANRVPAGGAMAVDR
                     EGYAESVTAELENHPLIDVIRDEITEIPDDAITVIATGPLTSDALAEKIHAVNGGDGF
                     YFYDAAAPIIDKSTIDMSKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPL
                     NSFEKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQ
                     LRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLL
                     TETFQSRNNPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKREEALVFPQTTAIGS
                     LPHYVTHADSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIASRALADLDTCLASL"
     gene            13666..15063
                     /locus_tag="VHD02_001427"
     CDS             13666..15063
                     /locus_tag="VHD02_001427"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HEQ5498028.1"
                     /translation="MQQQVAITETVLRDGHQSLMATRLSIEDMLPVLTILDKIGYYSL
                     ECWGGATFDACIRFLNEDPWERLRTLKKGLPNTRLQMLLRGQNLLGYRHYADDIVDKF
                     ISLSAQNGIDVFRIFDALNDPRNIQQALRAVKKTGKEAQLCIAYTTSPVHTLNYYLSL
                     VKELVEMGADSICIKDMAGILTPKAAKELVSGIKAMTNLPLIVHTHATSGISEMTYLK
                     VAEAGADIIDTAISSFSGGTSQPATESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRD
                     HYRETGILNPKVKDTEPKTLIYQVPGGMLSNLLSQLAEQGLADKYEEVLAEVPKVRAD
                     LGYPPLVTPLSQMVGTQALMNIISGERYKVVPNEIKDYVRGLYGQSPAPLAEGIKEKI
                     IGDEAVIACRPADLIEPQMIYLRDEIAPYARSEEDVLSYASFPQQARDFLGRREDPFY
                     DVPVQEVTVQLDIQD"
     gene            complement(15174..16226)
                     /gene="citC"
                     /locus_tag="VHD02_001428"
     CDS             complement(15174..16226)
                     /gene="citC"
                     /locus_tag="VHD02_001428"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HEQ5498029.1"
                     /translation="MPYYTVSKVFPSDKTTMASVKNLLHQEGIRLDAHLDYTCAIMNA
                     QNDVIATGSYFGNSLRCLCVSSAYQGEGLLNRIVSHLIDEEYALGNYHLFVYTKTSSA
                     AFFKDLGFTEIVHIDNHISFLENKKTGFQDYLMTLNKPEQTPGKVAAIVINANPFTLG
                     HQFLVEKAARENDWVHLFMVSEDQSLVPFSVRKMLIQEGLRHLDNIIFHETGPYLISQ
                     ATFPAYFQTEDNDVIKSQALLDTAIFLKIAQTLQITKRYVGEEPTSRVTAIYNEIMAE
                     QLQQAGILLDILPRKAINQQQDPISASTARQALKDNDWDLLAKLLPKTSLDYFCSLEA
                     QPIIKKIQATSSVKHY"
     gene            complement(16315..16797)
                     /locus_tag="VHD02_001429"
     CDS             complement(16315..16797)
                     /locus_tag="VHD02_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEQ5498030.1"
                     /translation="MPKRIRKALGVVGKLMSIVPDTTEIIGKTIDNSRPIIEKRMEQK
                     HEKEMQLRTIDDVINLPVDQAQAHLEQLGFVVATIPAKPHKKWLHSNLNEVVAMSPKS
                     GKYKIGSLIKLYYITVDVLEKSQDLLDQENLRTVERNQKIADTFESVKHIRFPFKKWR
                     "
     gene            complement(17491..18561)
                     /gene="xerS"
                     /locus_tag="VHD02_001430"
     CDS             complement(17491..18561)
                     /gene="xerS"
                     /locus_tag="VHD02_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEQ5498031.1"
                     /translation="MRRELLLEKIETYKAIMPWYVLDYYQSKLAVPYSFTTLYEYLKE
                     YKRFFDWLMDADLTQAPKIADIDLSTLEHLTKKDLEAFVLYLRERPSLNTYSTKEGLS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLASRAENIKQK
                     LFLGDETLAFLDYVDKEYEQKLSNRAKSSFRKNKERDLAIIALLLASGVRLSEAVNLD
                     LKDVNLNMMIIEVIRKGGKRDSVNVAGFAKGYLESYLAVRQRRYKAEKQDLAFFLTEY
                     RGVPNRMDASSIEKMVGKYSEDFKIRVTPHKLRHTLATRLYDATKSQVLVSHQLGHSS
                     TQVTDLYTHIVNDEQKNALDNL"
BASE COUNT         5895 a         3397 c         3654 g         5699 t
ORIGIN      
        1 ttgttgaaat gttttcgtat taatgttttt ttatctatta cgatagataa ttttacttca
       61 atttttgatt gaaaatacta aaataagtga tgtggtcact ttgacaacca tttattggtc
      121 cttttagata agacaatttt gtggctcttc agttgaaagg atgtgttgtt gatgttagag
      181 gaaacaatca tgttaaatta actcaggctt aaagaccaaa aaaggagtga agtctattat
      241 gaaaaaaaga aattttaaac ttacagaaat gttacaattg gttttattag tggttcctgc
      301 tgtaaaactt gctaaaaaaa tttttaacga tagtaaaaca ccgtctaaaa gaaagtaggt
      361 ctacaatgac taaaaaaaat catggcgttt tttgggggtt gttgcttgga tcagcagcag
      421 ctagcattgc ttacttaagt ttatcgtctt ccaaaaaaga tcaactcttg aaagatagtg
      481 ctaaaaaaat agatgatctc aacgcttact tgcaagataa aagtaagcaa gttctggatg
      541 cagtatcgga aaaagtacaa gaatccaaag atgctgttga agtttatggt ggtattgccg
      601 cagaaaccgt tgaagaaaag tggcaagata ccaaagatgc tgttgaagtt tacggtggta
      661 ttgccgcaga aacagttgaa gaatctttgg gacaggctaa agaaaaggta gaaggaattg
      721 gcgaagctac tagtcaaact atccaatcta aaatggaaaa actcaccagc gataagacag
      781 atgctgatga tgatgatgaa aaataggttt taaaatacct ttaagataag acaaaaagtg
      841 ccattaggca cttttttaat cttctgttgg aacaataatc gaaatggctt ttggaatgat
      901 atctaattga atgggcagat agtcactttt atccccatca atcctggtac gtggtaatat
      961 cgcttgtttt ttagcttgtg gtagtaagct aagcgtattg gcttgaaaat gactgatttc
     1021 ctctgctttg gaaaaatcac cctgtttaaa atcattaata tgccataaaa atttaaagaa
     1081 agagactttt ttcatggtat aaacttggaa atagccatcg tcagcttgag caccaggaga
     1141 aaaagaagga aaaccagcaa tggtatttgt catcgtgatt aagagaaact tcgtttttag
     1201 gtgaatacga tggttgtggc taattaaaga gatggcatag ctgcgatttc ttttcagtat
     1261 tcgaatacta tcttttaaaa atgctagagg accaaagcgt cgtttttcct cagccgttac
     1321 gtcggcagca atatcagcca gtaatcctaa ggttaaactg gaaatcatgt attgctggtt
     1381 agctttgcaa atatcaactt gtttgatttg tcccgtcaag atagtatcca acgcctcagt
     1441 aatttgtaaa gggatatgca tagccttggc aaaattattg actgtccctg agggcactaa
     1501 gcctattaaa caatgagctc ctccttcata gatgccaccg atgattttat ttagtgtccc
     1561 atcgccacca agagggataa ccaagtcaat cttgtcctta gcagcttgtt ttgctaattg
     1621 aaaagcctgg tcagcatctt taggggtaat aacttctaca gaatcttcgg aaaaaccatg
     1681 ttggcaaaaa tagtctttga cttgcccagc aagttggtct tctttctttc cagaattagg
     1741 attgtaaaaa atccgaacgg ttttcatcag gtctctcctt tgacagctta ttacttgcta
     1801 tccattatac cacttctaag aattgatgtc ttacaccaac tcatcacatt aagcctctca
     1861 atcagtctga gcatctattt atcttaaagc ataaagaaag tgatagcgat aaattggagg
     1921 gcagaggcca ataaaataaa gagatgccaa atcatgtgga agtagggtct ttttttggca
     1981 taaaataccg ctccaatggt ataagacaag ccaccaaata acatgagaag accaaaagca
     2041 agactagttt tttgaatgat gactggcaag ataaacacaa cgagccagcc cataacgata
     2101 taaagcatca aactgaattt ttcattgata cgtttagcaa aaattttgta taaaatccca
     2161 aaaagcgtaa tgccccactg caaaacaata atgatatagc ctagccaacc agaaacaagg
     2221 gataaggcaa caggtgtata acttcctgcg atagcaatgt aaatcatact gtgatcaata
     2281 atgcgtagaa tgtatttgtg aaccgaacca taagccatgg agtggtaaat cgtagaggac
     2341 aaaaacatca aaaagagact gatgacaaag atggagatac caatagcggc tttaaggtca
     2401 taagtttgat aagcatagct ggctgaaata ggaagtagaa tgagcatagc aaatgctccg
     2461 atagcgtgtg tcacgctgtt tgctacttcc tcactaaaag ataagggtaa actttgttta
     2521 aaggtttggt tcattgggtc acctcctgta ataaataaat agttttgtgg taatctttga
     2581 ccgtttgaca ggcaacctgc acaatcggtg gaatgtcctc ctcaatggga gccttctgat
     2641 tccaacaggt tacaaaagta ttgtaaagac tttcagaatc agcctgtgcc tgaagttcct
     2701 ccaatacgcg actaatatct tgaataggaa ataccgtttt aaacaaactg atggcaatta
     2761 agcgcgctag ttgttgcttt tggtattttt tcttgatagg tttagtcaca tacccatgct
     2821 tgacgtaatt attaatcata gatgccgtta gtgatttatt atctgacact tctgaaaaat
     2881 cagtgcactg gttgacataa agtaagactt gatccaaata taagtctaaa tcagggaggt
     2941 ccttccaata aggtaatgat ggaaaaatca tagtttcgtc tctctatcta gttttgataa
     3001 ctagattata tgattaatga aaacttattg caagcaaaaa ctgtaaagac ttaaaagagg
     3061 aaagtagaaa tggcgaagtg aagctaatca tgctataatg ttaaagaatt gattgggata
     3121 gatgccttct gtcgcaatca gactatttta tcacgaagaa gatgatgcta cgaaaggaat
     3181 ttaaactcta tggctactat tcaatggttt cctgggcaca tgtccaaagc cagaagacag
     3241 gttcaggaaa atgtaaagca tgttgacttt gtaactattt tagtggatgc acgcttacca
     3301 ctttctagtc aaaatcccat gcttacaaaa attgttggtg ataaacctaa attaatgatt
     3361 ttaaataaag ctgacttggc agatgcaact cgaactaaag agtggaaggc ttattatgaa
     3421 agtcaaggta ttaaaacatt agccatcaat tccaaagaac aatcaacggt taaaaaagta
     3481 acagaagcag ccaaggaact catggctgat aaaattcaac gcttgcgaga gcggggcatt
     3541 caaaaagaaa ccttgcgaac catgattatt ggcattccaa atgctggtaa atctacctta
     3601 atgaatcgct tggctggtaa gaaaattgct gtagttggca ataaacctgg tgtgactaaa
     3661 ggacaacaat ggttgaagtc caataaggaa ttggaaattc tagacactcc tggtatttta
     3721 tggccaaaat ttgaggacga attggtgggc ttaaaattag ctttaacagg tgctatcaag
     3781 gaccaattac taccaatgga tgaggtcact atttttggtt tgaattactt taaaacacat
     3841 tatcctagtc gccttactaa gcgttttaag aatattcctc ttgaggaaga agctcctgaa
     3901 atcattatga ccttgacacg ccaactggga tttaaagatg attacgaccg tttctacact
     3961 ttattcgtca aggaagttcg tgatggtaaa ttaggtcaat acaccttaga tcaggtggga
     4021 gatatggatg tcgactagta ttaaagctat taaagaaagc ctggaagctg ttactagcct
     4081 cttggacccc ctctttcaag aattggcaac cgacactagg tcaggcgtcc aaaaagctct
     4141 aaaaagccga caaaaggcta ttcaggccga gttagcagaa gaagaacgat tagaagccat
     4201 gctttcttat gaaaaagctc tttataaaaa aggttatcaa gccattgcag gtattgatga
     4261 ggtgggacgt ggtcccttag caggtcccgt tgtggcagct tgtgtgattt tacctaagta
     4321 ttgtaaaatt aaaggcctta atgattctaa aaaaatccct aaatctaagc atgagaccat
     4381 ttatcaggca gtgaaagaaa aggctttggc tatcggtatc ggtattattg acaatcagct
     4441 tattgatgag gtcaatattt atgaagcaac caaactagct atgctagaag ccattaaaca
     4501 gttagagagc caactcacac agccagatta tctcttgatt gatgccatga cattggatat
     4561 tgctatttcg cagcagtcta ttcttaaagg cgatgccaat tccttgtcta ttgcagcagc
     4621 atcaattgta gctaaggtca ccagagatca gatgatggct aactatgatc gcatttttcc
     4681 tggttatggc ttcgctaaaa atgcaggcta tggcaccaaa gaacatttac agggattaaa
     4741 agcttacggc ataacgccta tccatcgtaa aagttttgaa cctgttaaat ccatgtgctg
     4801 cgattcaact aatccttaaa aagttctgtt tttagcagaa tttttttctt ttttacgaat
     4861 aaatgaagta aggaggtaac ttagtgaatc attttgaact ttataagcta aagaaagctg
     4921 gactgactaa taaaaacatt ctcaatattc ttgactacca agaacaccag gaaaaatcgt
     4981 tgtcacttcg agatatggcc gttgtttctg gttgtaagca tccgtctcac tttatagaag
     5041 cctataagca gttagatatt caaaatttaa aaatagaatt taaacagttt cctagtattt
     5101 ctatcttaga taagcattac ccaatggcat tgaaagaaat atacaatcca cctgtcctct
     5161 tgttttttca gggaaattta gaccttcttg agaaacctaa attagccatt gtcggctcca
     5221 gacgctcaag cgataccgga gtaaagtctg tccgtaaaat tcttaaagaa ctcgggaacc
     5281 gttttgtgat tgttagcgga cttgctcgag gtatcgacac tagtgctcat ttagcctgcc
     5341 ttaaaaatgg gggacaaacc atcgctatta ttggaacagg gttggatcgc ttttacccta
     5401 aagaaaatag ggagttgcaa actttcttag ggaaaaatca tcttgtgcta acagaatacg
     5461 gtccaggaga agaagcttta tcttatcact ttccagaacg gaatcgtatt atcgcaggcc
     5521 ttagccgagg tattcttgtc gttgaagcaa aaaatcgttc aggttccttg attacttgtc
     5581 aaattgggat agaagaaggc cgagacattt ttgctgtccc aggaaacatt ttggacggga
     5641 aatccgaagg ttgccttcag ttaattaaag agggagcaac atgcgtcacg tcgggcatgg
     5701 atatcctttc agagtactaa aaaagttaaa gtttcttcct atgtagcatt tttttattga
     5761 cagtttctta aaaatgggat attatgctat aagctttaaa caaactgtta taggaagtgt
     5821 gtcgaattgg gaacaaaaac tacaactaag ccaaaaacgg gtactaaaaa gtccaccact
     5881 aaaaagaaat caatagcgaa aaaaaatctc gttattgttg aatcacctgc caaagctaag
     5941 actattgaaa aatatcttgg acgcagttat aaagttgttg cttcggtagg acatattcgt
     6001 gacttaaaaa aatcgtctat gtctattaat tttgataata attatgagcc acaatacatt
     6061 aatatccgtg gtaagggacc ccttatcaat tctttaaaaa aagaagccaa agctgctaaa
     6121 aaagtttatt tagctagtga cccggatcgt gagggagagg ctatttcttg gcatttgtca
     6181 catatcttag gacttgatcc tcaagataat aaccgagtgg tttttaatga aattacgaaa
     6241 gatgcagtta aacatgcctt tgtggagcct cgtcagattg atatggactt ggtagattct
     6301 caacaagcac gtcgtgtttt ggaccgtatt gtgggatatt ccatttcacc aattctttgg
     6361 aaaaaggtca aaaaaggact ttcagcagga cgtgtccagt cggttgcttt aaaactcatc
     6421 attgaccgtg aaaatgacat caaagcattt gttccaaagg aatactggtc tattgatggt
     6481 ctttttaaaa aaggaactaa aaagtttcaa gcgacttttt acggtattaa tggcaaaaaa
     6541 acaaagcttg ataataacaa cgatgtcaaa gaggtattag caaagctaac aaatgaagat
     6601 tttcttgtca gcaaggttga caagaaagag cgacgtcgta atgctccctt accttacaca
     6661 acatcatctc ttcaacaaga tgctgctaat aaaatcaatt tcagaacgcg taaaaccatg
     6721 atggtagccc aacaactgta tgaggggatt catttaggag aaaatggaac tcaaggttta
     6781 attacctata tgcgtactga ttctactcgt atcagtccag tagcacaaaa cgatgctgct
     6841 caatttatta tcaatcgctt tggagctaat tattcaaaac atggtaatcg tgtcaaaaat
     6901 accagtggtg cccaagatgc tcacgaggcg attcgtccat ctagtgttaa tcatacgcca
     6961 gactctattg ccaaatacct caacaaggat cagttaaaac tttatacgct tatttggaat
     7021 cgttttgtgg ctagccaaat gacagcagct gtttttgata ccgtaaaggt gaacttggag
     7081 caaaatggcg tgatttttgt tgccaatggt agccaaatga agtttgatgg ttacatggct
     7141 gtttacaatg attccgataa aaacaaaatg ttacctgaaa tggaagaagg agaaaacgtt
     7201 aaaaaggtat caacttctcc agaacagcac ttcacgcaac ctccggctcg ctactccgaa
     7261 gcaactctga ttaaaacttt agaagaaaat ggggtcggtc gaccatcaac ttatgcccca
     7321 accttagaag tgattcaacg caggtactat gttaaattat ctgctaagcg ctttgagcca
     7381 actgagcttg gggaaattgt caacaagctt attgtcgaat ttttcccaga tattgttgat
     7441 gttgccttta ctgccgagat ggaaggtaag ctagatcaag ttgaaatcgg tgaagaagaa
     7501 tggcaacatg tcatcgatca attctatcaa ccctttgtca aagaattgaa taaggctgag
     7561 tctgaaattg aaaagattca aatcaaagat gagccagctg gttttgactg tgatgtttgt
     7621 ggccacccaa tggtcattaa gttaggacgt tttgggaaat tttacgcgtg tagcaacttc
     7681 ccagagtgcc gtaatacaaa agccatcact aaagaaattg gtgttacttg tccggtttgt
     7741 cataaaggac aagttatcga gcgtaaaacc aaaaaaaata ggatatttta tggttgtgat
     7801 cagtatccag attgtgagtt tatctcgtgg gatttaccta ttggacgtgc ttgccctaag
     7861 tcaggtgact atcttattga gaaaaaagtt cgtggtggca aacaagtaat gtgtagcaat
     7921 gagacctgtg attacaagga agaaaaaatt aaataaaaaa agaattggaa ctccttgtct
     7981 tgttccaatt ctttttttat ttacaaaggt atttttaact acaaaaatcg ctagttcgtt
     8041 tttaaaaggc ttaaaaattc ctcaataact ggactgaaac tcgcatcatc tcggtaagca
     8101 atcccaatag ggtaaaaatt atttttatct tgcaaaggaa tccaaactaa gttatctacc
     8161 tgattaagac tctcaaaatc actcgggagg atagtcaagg tatttaaatc gtgcaaacta
     8221 tgaataagaa cttcccaatc attgtgttta aagacaatat ctggttcaaa accgagagct
     8281 cggcattttc taggaagcat ttcgcctaac atgtagtgat cgtttaaaga agcaatctta
     8341 tagcctttta aatctttaag ttttaactga ggatgttgcg ccaaagggtg ctgtttgagg
     8401 agaacgatac tgaccttata gccttttgtg gacgtttgta atagctcaat ggtaatatcc
     8461 ttttgaatac ttagaaagga gaggagacca atatcgattt ttccttggga caataattct
     8521 tataatttgc gagaaccatc ctgtataagt gatacttcaa catttgagtg agtagccata
     8581 aagattgaaa tttgtttcat gaattgtata gcaaacagtg atgttaattc aacacgaatc
     8641 atctcttttt ttcttgattt aattgctgaa tatccgcaac aatttgatta aagtcatcca
     8701 caaggtcttt gcctttgtga taaagaatat ctccagcttc ggttagtagt agctgtcaat
     8761 cactttgtgt aaaaagcgtg gtgttcaaac taggactcta gccttttaat tgattgagaa
     8821 agagtatgtt gcgttacaaa caaactttta gcagcgtggg aatgattttt tgtttcagca
     8881 actgcaataa aataggttaa ttatctaata tccatagtag atccttcatg attagtcagg
     8941 gaattgcgtc tctacttggt ttacaaccta ttttgatgtc tctccttatt gccatgattt
     9001 tctgcttcct tattgtttct ccaattacaa cggtaggaat tgctcttgcg attaacttat
     9061 ctggtattgg ttcaggtgct gcaaacttag gtatttgtgc cgcaagcttt ggactttgtt
     9121 tagcaggttg ggctgttaat tcaaaaggaa cttcacttgc tcacgtcttg ggatcaccga
     9181 aaatttcaat ggccaacgtt ttatcaaaac caaaaatcat gttgccaatg ttatgttctg
     9241 cagctgtact tggtgttatt ggtgctatct ttaatattca aggaacacct gctagtgcag
     9301 gctttgggat tagttgtttg atcggaccta tcaatgccct taatttagct aaaggcggat
     9361 ggtctctagt aaatatccta cttatcatta tcatttttgt aagggcacca attgttctca
     9421 atatgatctt taactacctt tttatcaagg tgcttaaagt gattgatcca atggattaca
     9481 aacttgatat ctaagaaagg aacttttgat gaaattaaaa ttatataatg ttagaggcga
     9541 agaagcagtc ttagcaaaaa aatgggctga tgacaatggg attgagattt ccttgacaga
     9601 gtcttcctta actcctgaaa cggttaaaga agctgaaggc tttgacggga ttgccaatgc
     9661 ccaaattggt cctttagatg atgcaatcta tcctctctta aaggaaatgg ggattaaaca
     9721 aatcgctcag cgcagtgctg gtgtcgatat gtataacctt gatttggcca ccgaaaatga
     9781 cattattatc acaaatgttc ctagctattc tccagaatcc attgcagaat ttacggttac
     9841 tattgccctt aatttaattc gtcatgtgga gttgattcgt gaaaatgtta aaaaacaaaa
     9901 tttcacttgg agactcccta tccgcggccg tgttttaggt gatatgacag ttgcgattat
     9961 cggaactggc cgtattggtc tagctactgc taaaatcttt aaaggttttg gctgcaaggt
    10021 cgttggatat gatatttacc aaagcgatgc tgccaaagct gtcttggact ataaggaatc
    10081 tgtagaagaa gcgattaaag atgctgatct tgtttcctta cacatgccac caactgcaga
    10141 gaacacacac cttttcaatg ctgatttatt taaatcattc aaaaaagggg ctattttgat
    10201 gaatatggcg cgtggtgctc tcattgagac gcaagattta ctggatgctt tggatgcagg
    10261 cttattgagc ggagctggta ttgacactta cgaatttgaa ggaccttata ttcctaagaa
    10321 ttttgaaggt caagaaatta cagattcact atttaaagct ttgattaacc atcctaaggt
    10381 tatttatacg cctcatgcag cttattatac tgatgaagcc gttaaaaacc tagttgaagg
    10441 tgcccttaat gctacagtag agattatcaa aactgggaca acgacaactc gtgttaacta
    10501 acaactgatt tgacgtccat tataaaggaa taattccaaa aattccttta taatggactt
    10561 ttttgtatta gtccgttata atagactgta agaaaggggg ctcttatgac atatatagcg
    10621 cttattggag atattatcca gtccaaacaa ttgacggatc gttccaaagt tcaaaaaact
    10681 ttagcagctt atttagacga cctcaacaaa acctttgctc cttatattat ttctaaatta
    10741 tccctaacgt tgggagatga atttcaagga ctttttcagg tggatactcc tattttccat
    10801 ctgattgatt tgattaatca tcacatggat atccctattc gatttggagt aggagtaggt
    10861 tctattctaa cagacatcaa tcctgatatt agtattgggg ctgatggacc tgcttattgg
    10921 catgctcgtg aggctattcg ttatattcat caaaaaaatg attatggtaa tactacatta
    10981 gctcttcgga ctggtcatca caaccaggat gatgtattaa acagtttact agcagctgga
    11041 gatgctatta aagccaactg gcgagctagt caatgggaaa tttttgacac gcttttagat
    11101 ttgggcattt atgaggaata ctttgatcaa caacgactag gaaagcaatt atctcttagc
    11161 tctagcgctt tgtcaaaacg tctaaaaagt agtcatgtta aaatttatct gagaacacgg
    11221 cagagtgcgc taaactgtct caaacaaatc aaggaggaaa ctgatgatta atggtgtctc
    11281 tcactaccta gctcaaaccc ctacacttac cctgtttctc atttgtcatg tgttatcaga
    11341 ctaccagtta caaagtcagc aagtagctga tttaaaagaa aaacatttga cttatttagg
    11401 ttaccacttg attggagtta gcattcccct aatttgttta accctcatta tcccacaagc
    11461 atggttgatg agcctattgg ttatgataag tcacgcgctt attgactggc taaagccaaa
    11521 gatggctaac tctctaaaat ggaaaagaga gtggattttt ctgctggatc aatgcttaca
    11581 tatcgctatt agttcatttg ctgggctaag gttagcaggg gtgaccttgc ctaattggct
    11641 tccgatatct attttgatga ccgttttatt tatcctagtc attaccaaac caactaatat
    11701 tgtctttaaa ctctttttca gcaaatacca gcctgaccaa ggagaaaaaa tggacacgat
    11761 cataggagcg ggggctacca taggaattct agagagaatt gttattggtt tgtgtatgat
    11821 catggggcaa tttgcttcaa taggtctggt ctttaccgct aaatctattg ctcgctacaa
    11881 taaaatttcg gaaagtccag cttttgcaga atactacctg attgggtctt tatttagtat
    11941 tctcagtgtt tttatcgcag catggatttg tttcttctag ttgaaaaggg ttcccgtaga
    12001 aagaggttat cgcttagtct ctttctcttt ctctttcttt ttctttcttc ttatttctgt
    12061 tataataagt caggtaacta cattagaaag atttgtgggt ctgtcatcta agagatgtca
    12121 ttcccgaaga ggtattagtg acttgtctca atcaactgca acttatatta atgttattgg
    12181 agctgggcta gctggttctg aagctgccta tcagattgct aagcgcggta tccccgttaa
    12241 attgtatgaa atgcgtggtg tcaaagcaac accgcaacat aaaaccacta attttgccga
    12301 attggtctgt tccaactcat ttcgtggtga tagcttaacc aatgcagtcg gtcttctcaa
    12361 agaagaaatg cgtcgcttgg attccattat tatgcgtaat ggtgaagcta atcgtgtacc
    12421 tgctggagga gcaatggctg ttgaccgtga ggggtatgca gagagtgtca ctgcagagtt
    12481 ggaaaatcat cctctcattg atgtcattcg tgatgaaatc acagaaatcc ctgacgatgc
    12541 cattacggtt atcgcgacgg gaccgctgac ttcggatgcc ctggcagaaa aaattcacgc
    12601 ggtaaatggt ggcgatggat tctatttcta cgacgcagca gcgcctatca ttgataaatc
    12661 tactattgat atgagcaagg tttacctcaa atctcgctac gataaaggcg aagctgctta
    12721 cctcaactgc cctatgacca aagaagaatt catggctttc catgaagctc tgacaaccgc
    12781 agaagaagcc ccgctgaatt cttttgaaaa agaaaagtat tttgaaggct gtatgccgat
    12841 tgaagttatg gctaaacgtg gcattaaaac catgctttat ggacctatga aacccgttgg
    12901 attggaatat ccagatgact atacaggtcc tcgcgatgga gaatttaaaa cgccatatgc
    12961 cgtcgtgcaa ttgcgtcaag ataatgcagc tggaagcctt tataatatcg ttggtttcca
    13021 aacccatctc aaatggggtg agcaaaagcg tgttttccaa atgattccag ggcttgaaaa
    13081 tgctgagttt gtccgctacg gcgtcatgca ccgcaattcc tatatggatt caccaaatct
    13141 tttaaccgaa accttccaat ctcgtaacaa tccaaacctt ttctttgcag gtcagatgac
    13201 tggagttgaa ggttatgtcg aatcagctgc ttcaggttta gtagcaggaa tcaatgctgc
    13261 tcgtttgttc aaaagagaag aagcgcttgt ttttcctcag acaacagcta ttgggagttt
    13321 gcctcattat gtgactcatg ccgacagtaa gcatttccaa ccaatgaacg tcaactttgg
    13381 catcatcaaa gagttagaag gcccacgcat tcgtgacaaa aaagaacgtt atgaagctat
    13441 tgctagtcgt gctttggcag atttagacac ctgtttagcg tcgctttaac actaaaaaag
    13501 agttcctttt cagttgagtt gctgaagagg acttttttac ttgctgattt gagcgctagc
    13561 tatgcaaaag aatgcacaaa aaatacatga atagactatg gtcactagaa gcaatattat
    13621 ataatgaata gtactcatta gttgattcag tttggaggtt ttagcatgca gcagcaagtc
    13681 gcaataacag aaacagtttt acgggacggt catcaaagtt tgatggcaac gcgtttatct
    13741 attgaggata tgcttcctgt ccttaccatc ttagacaaga ttggttatta ttccctagag
    13801 tgttggggag gagcaacctt tgatgcctgt attcgttttt tgaacgaaga cccttgggaa
    13861 agactgcgca cgttaaaaaa aggcttacct aatacccgtt tacaaatgtt attaagaggc
    13921 caaaaccttt taggctaccg tcattatgca gatgacatcg ttgacaaatt cattagttta
    13981 tcagctcaaa atgggattga tgttttccgc atttttgatg ccctaaatga cccacgaaat
    14041 atccagcaag ccttaagagc tgtgaaaaag acgggtaaag aggctcaact gtgtattgcc
    14101 tatacaacta gcccagtgca cacgcttaac tattaccttt ctttagtgaa agaattggtt
    14161 gagatgggag ctgattctat ctgcatcaaa gatatggctg gtattctaac tcccaaagcg
    14221 gctaaagagt tagtatctgg tatcaaagcc atgactaact tacctcttat tgtgcatacc
    14281 catgctacca gtggtatttc agaaatgact tacttaaaag tagcagaagc aggagctgat
    14341 attattgata cggcgatttc ctctttttca gggggaacca gtcagcctgc cacagaatca
    14401 atggcgattg ccttgacgga tttaggcttt gacacaggct tggatatgca agaggtagcc
    14461 aaagttgcag aatatttcaa taccattcgt gaccattacc gagagacagg gattttaaat
    14521 cccaaagtca aagacactga gccaaaaaca ttgatttacc aagttccagg tggtatgcta
    14581 tcaaacctat tgagccaatt agccgagcaa ggcctagctg ataaatacga agaagtctta
    14641 gcagaagtac ctaaagtaag ggctgatctt gggtatccgc cactcgtaac gccattatca
    14701 caaatggttg gcacacaggc cttgatgaat atcatctcag gggaacgcta caaggtagtt
    14761 ccaaatgaaa ttaaagacta tgttcgaggg ctatacggtc aatcaccagc gccattagca
    14821 gaaggtatca aagagaaaat cattggtgac gaagcagtca ttgcttgcag accagccgac
    14881 ctaatcgagc ctcaaatgat ttatctacgt gacgagattg ctccatacgc tcgttcagaa
    14941 gaagacgtgt taagctatgc gagtttccca cagcaagcca gagatttctt gggacgccgt
    15001 gaagatcctt tctatgatgt tccggttcaa gaagttactg tacaactgga cattcaagac
    15061 taacagggaa tgactaatcc tcttaaccat taatctcctt ggtgacacgc atttgctgta
    15121 agaacaatac caaaaaccta cctgattttg ataatcagat aggttttttt atcttagtaa
    15181 tgtttgacag atgaagtggc ttgtatcttt ttaataatgg gttgggcttc tagcgaacaa
    15241 aaataatcta aagaagtttt gggaaggagt tttgccagaa ggtcccaatc attatctttc
    15301 aatgcctgcc tagccgttga ggcactgata ggatcttgct gctgattgat agctttcctt
    15361 ggcaaaatat ctaggaggat accagcttgc tgcaactgct ctgccatgat ttcattgtaa
    15421 atagcagtta ctcggctggt tggctcttct ccaacgtatc tttttgtaat ctgtaaggtt
    15481 tgagcaatct ttagaaaaat agcagtatcc agcaaagctt ggcttttgat cacatcattg
    15541 tcttccgttt ggaaataagc tggaaaggtt gcctgactaa tcaaataagg acctgtttca
    15601 tgaaagataa tgttatcaag atgtctcaat ccttcttgaa tcaacatttt tcgtactgaa
    15661 aaaggaacca agctttggtc ctcactaacc ataaacagat gaacccaatc attttctctt
    15721 gcagctttct ctactaaaaa ttgatgccct aaggtaaagg ggttagcgtt gataacaata
    15781 gcagcgactt taccaggtgt ttgctcaggc ttattaagtg tcatcagata atcttgaaaa
    15841 ccagtttttt tattttccaa gaaactgatg tgattgtcga tatggacaat ttcagtaaaa
    15901 ccaagatctt tgaaaaaagc agcagaagag gtcttcgtat aaacaaaaag atgatagttg
    15961 ccaagggcat attcttcatc aatgagatga ctcacaatcc tattaagaag tccttcacct
    16021 tgataggcac tggatacgca aagacagcgc aagctatttc caaagtaaga accggtagca
    16081 atcacatcat tttgcgcatt catgatggca caagtataat ctaaatgagc atccaaacga
    16141 ataccttctt gatgaagcag gttctttaca ctagccattg tcgttttatc tgaaggaaag
    16201 acttttgaaa cagtgtagta aggcatttct tctccttatc tttaatttta ttgaatcata
    16261 acataactta gtaccaaaac aggctgatct atctaaaggc aactctgttt tttatcaacg
    16321 ccatttttta aaaggaaatc gaatgtgttt gacagactca aaagtatcag cgattttttg
    16381 attgcgttcc acagtccgca agttttcttg gtctaataag tcttgacttt tttccaaaac
    16441 atcaacagta atgtagtaga gtttgatcag gcttcctatc ttatatttac cagattttgg
    16501 agacatagca accacttcat tgagattact atgaagccat tttttatgag gcttagcagg
    16561 tatagtggct actacaaagc caagttgttc caaatgagct tgtgcctgat caacaggaag
    16621 attaatcaca tcatctatcg tgcgtagttg catctctttt tcatgttttt gttccatacg
    16681 tttttcgata attggtcgac tgttatcaat cgtttttcca ataatttcag tagtgtcagg
    16741 aacaattgac atcagtttcc caactactcc tagagctttt cttattctct ttggcatctt
    16801 ttatctcctc aagtcgtttc tttttattat aacaaattgt ctaaaaatag tatgcgataa
    16861 accttgttct aaaagattaa atagatcaat tcaaggaaaa aataggaggg gatccgacaa
    16921 aaaatacaaa gattctaaaa aagtcaagct attttagcct ttgtcaatcg tatcagttca
    16981 aatagaaaat acaagttttg acaagaattt atttatgtaa caaactttgt ttttgattct
    17041 tcagactatc tagcgataat gttatctgat tcttgttttc aaccaattga tgttaaagaa
    17101 gctgtcttca tgattctcta acattccttc agttttagct ttatattact ctaagttatc
    17161 agtaatgaca ttaagtagtt taataagaat aatcctaaaa gctactttac aaaataacgt
    17221 attatcatac taaataaaag taccgacata ctaaaaacag gcttatctca tctttttgat
    17281 gagaacctta ctagatttaa tctataaaca tcacaaaaaa gtcattcaaa gaagggacaa
    17341 agttagcagt gatattcctt tttctaagtg gcttgagtag tttggaaaac ggcctaacac
    17401 caaactgtca cggttaatag tagccatcta ttaaacttaa ttttgaagct atttttacct
    17461 ttaatgttac ataaaaacaa ttatgtaaca ttaaaggtta tctaaagcat ttttttgctc
    17521 atcattgaca atatgggtat aaagatccgt gacttgagta gaggaatggc ctaattgatg
    17581 actaactaac acctgagatt tagtagcatc atatagtctt gtcgctagcg tatgacgcaa
    17641 tttgtgagga gtcacacgaa ttttaaaatc ctcagagtac ttgccaacca tcttttctat
    17701 actagaagca tccattcgat ttggaacgcc acggtattct gttaaaaaga aagcaagatc
    17761 ctgtttttca gccttgtaac gtctctgacg aacagctaaa taagactcca aataaccttt
    17821 cgcaaaacct gctacattga ctgaatcacg ttttccgcct ttgcgaataa cttcgataat
    17881 catcatattt agattgacat cttttaaatc aagattcact gcttcagaca aacgaacacc
    17941 agaagcaagt agtagagcaa taatagccaa atccctctcc ttatttttac ggaaagaaga
    18001 tttggcacga ttagacaatt tttgctcata ttccttatcc acataatcca agaaagctag
    18061 agtttcatca cctaaaaaca gtttctgctt aatattttcc gctctggaag ccagggtttc
    18121 ttttttcttt tttgttgaca cttttttcat aacattccga taaaaataag gctccccttg
    18181 gtcattttca acctcttcag tcaagtactt gtaaaggctt gatagggcag acaaggtccg
    18241 attaatagtg gtttgagaaa gcccctcttt ggtggaatag gtgttgagag aaggtcgttc
    18301 ccgcaaataa agcacaaagg cctctaaatc cttcttggta aggtgctcaa gcgtgctcaa
    18361 gtcaatatca gcaatctttg gcgcctgcgt taaatcagca tccatcagcc aatcaaaaaa
    18421 gcgtttgtat tcttttaaat actcatataa ggtggtaaag ctgtatggaa cagctaactt
    18481 ggattggtaa taatctaaaa cataccaagg catgatggcc ttataggttt caattttttc
    18541 tagtaataat tcgcgtctca tatcatcata attccttaac aataacaatt ttttcaaaaa
    18601 tgaattttcg caaattgtct catgtatttt atgtcattca ttctc
//
LOCUS       DAUDYX010000043        17150 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.043, whole genome shotgun sequence.
ACCESSION   DAUDYX010000043 DAUDYX010000000
VERSION     DAUDYX010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17150
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.043"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(5..1996)
                     /gene="uvrB"
                     /locus_tag="VHD02_001431"
     CDS             complement(5..1996)
                     /gene="uvrB"
                     /locus_tag="VHD02_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEQ5498032.1"
                     /translation="MIDKRDDKPFKLKSKYKPSGDQPQAIESLVDNIEGGEKAQILLG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSAVSLRPGQEISRDTLLNQLVDIQFERNDIDFQRGCFRVRGDVVEVFPASRDEHAFR
                     VEFFGDEIDRICEIESLTGKTIGEVDHLVLFPATHFVTNDEHMEQSIAKIQAELAEQL
                     QLFESEGKLLEAQRLRQRTEYDIEMLREMGYTSGVENYSRHMDGRSPGEPPYTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDQARKQMLVDYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGEYEMSQTNTIIEQIIRPTGLLDPEIDVRPSMGQMDDLLGEINQRVARD
                     ERTFITTLTKKMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNVDGHVIMYADKMTDSMQ
                     RAIDETARRREIQIAYNKAHGIVPQTIKKDIRGLISISKTSHNDISKEEMDYESMSRG
                     ERKEAINALQKQMQEAAELLDFELAAQMRDLILELKLMD"
     gene            2189..4363
                     /locus_tag="VHD02_001432"
     CDS             2189..4363
                     /locus_tag="VHD02_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEQ5498033.1"
                     /translation="MTHKIKVLLLAIMSIFLTCNIASAETIAIVSDTAYAPFEFKDSD
                     QIYKGIDVDIINEVAKRQSWDFSMSFPGFDAAVNAVQSGQASALMAGTTITNARKKVF
                     HFSEPYYDTKIVIATRKANAIKKYSDLKGKTVGVKNGTAAQAFLNNYKKKYDYTVKTF
                     DTGDLMYNSLSAGSIAAVMDDEAVIQYAISQNQDIAINMKGEPIGSFGFAVKKGSGYD
                     YLVNDFNTALKAMKADGTYQAIMTKWLGTDDKATTSQATGNPSAKATPIKDSYKIVSD
                     SSFAPFEFQNGKGKYVGIDIELIKAIAKQQGFKIEIANPGFDAALNAVQSSQADGVIA
                     GATITDARKAIFDFSDPYYTSNIILAVKAGKNIKNYEDLDRKTVGAKNGTSSYSWLKE
                     NAPKYGYNVKAFDDGSSMYDSLNSGSVDAIMDDEAVLKYAISQGRRFETPLEGISTGE
                     VGFAVKKGTNPELIEMFNNGLAALKKSGQYDDIIDKYLDSKKAATPSEKGADESTISG
                     LLSNNYKQLLAGLGTTLSLTLISFAIAIIIGIIFGMMAVSPTKSLRLISTVFVDVVRG
                     IPLMIVAAFIFWGVPNLIESMTGHQSPINDFLAATIALSLNGGAYIAEIVRGGIEAVP
                     AGQMEASRSLGLSYGTTMRKVILPQAVKLMLPNFINQFVISLKDTTIVSAIGLVELFQ
                     TGKIIIARNYQSFRMYAILAIIYLIMITLLTRLAKRLEKRLN"
     gene            4363..5103
                     /locus_tag="VHD02_001433"
     CDS             4363..5103
                     /locus_tag="VHD02_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ5498034.1"
                     /translation="MTELKIDVQGLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLETITSGKVMVDGFELSDPKTNIDKARENIGMVFQHFNLFPHMTVLENI
                     IFAPVELGKESKEVAKKHGMALLEKVGLSDKADAFPGSLSGGQKQRVAIARSLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGQF
                     LEDGTPEEIFDHPKHPRLIEFLDKVLNV"
     gene            5261..6748
                     /locus_tag="VHD02_001434"
     CDS             5261..6748
                     /locus_tag="VHD02_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEQ5498035.1"
                     /translation="MLHKEKPDYNRNQYGFYTLDQIVPDEHFLRQVEAVIDFDFIYDL
                     VEDTYSSDNGRPSLDPVMLVKIPLIQCFYGIRSMRQTIKDIEVNTAYRWFLGLTLDNK
                     VPHFTTYGKNYSRRFQEKGLIESIFTHILGLCINTGLIDPMEIFIDGTHIKAAANNRK
                     FINREVEKQAKFMSEQLEIEINRDREKHGKKPLGPVAKKISTTDPESGWFHKGDHKEV
                     FAYSAQVACDKYGWALAYSVEAGNIHDSQAFPALFAKLEPLKPQFIIADSGYNIPSIA
                     KFLIDKEITSVLPYTRPRGKKDLLRPKEFIYDEHFDCVICPEHQALTYRTTTREGYRE
                     YKSDPVICANCPLLSVCTTSKNHLKVVTRHIWKDYLEQCEDIRHQRGMKELYQHRKET
                     IERLFGTAKEYHNLRYTREKGKSKMEDKVGLTLACLNLKKRVKRMVGEPFYFVQMRWF
                     KHGNRHFYLKTLKKTNTKSMFVFNLRQGNRFPCLFWLLLLFKPAK"
     gene            complement(6797..6949)
                     /locus_tag="VHD02_001435"
     CDS             complement(6797..6949)
                     /locus_tag="VHD02_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498036.1"
                     /translation="MTEKNGATFLMAVGFFLIAISPMVGNKYSHIVTGLALTGLGYYL
                     TKHKRP"
     gene            complement(6946..8325)
                     /locus_tag="VHD02_001436"
     CDS             complement(6946..8325)
                     /locus_tag="VHD02_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEQ5498037.1"
                     /translation="MSHFSDKFMDISGKIGSQRHLVAIRDSFISMMPITMAGSVAVLL
                     NVFLRDIPNNMGWTGFAKAMQPVIDINGYVYFGTIGIMALFFAFAFGYNLAVMYKINP
                     LAGGLISFGSFIATLPQTLTISTPLENASANLISNLKEMGLSVVTAGGGSSIETSQWG
                     AIALKYVGATGLFTALIIGFLSSFVYAALTKRNITITLPDNVPPAVNKAFAAIIPGTV
                     AIYASAIFAYLIFALTGSSLSDVISTYIQLPLLGLSQGIGSVILLTFLVQLLWFFGLH
                     GHNVLAPVMDGIYMVALTENTAAYNTAHSAANLPYLWTRGSFDAYAQMGGSGVTLALI
                     IAIFIFSKREEHKTIAKLSAPMGVFNINEPITFGMPIVLNPTFVIPWLIVPPICASIA
                     YFATAIGLIPPVFLSVPWITPVGLYAYLATGGNIMAGLVSLFNLFVAFLIWAPFVILA
                     NKEKASDLA"
     gene            complement(8503..8952)
                     /locus_tag="VHD02_001437"
     CDS             complement(8503..8952)
                     /locus_tag="VHD02_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEQ5498038.1"
                     /translation="MIISAEGDVSVHHCFEAMLISLQEDHRQNTLRQLSRDDIKAGIN
                     YVKHYGKYKERTVRVLITALEAPQLYTACFSSNHGIKELAYRLEALGENRTRITYSYD
                     YHPLDIFQKANHFIVGKLFKKSLERQSQAQLAALIQYAKQLQQPSSL"
     gene            complement(8949..9284)
                     /locus_tag="VHD02_001438"
     CDS             complement(8949..9284)
                     /locus_tag="VHD02_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEQ5498039.1"
                     /translation="MTEPSNLEAIMGLIMYGGEAKGKAVEAIHAARDGQFEEAQHLLQ
                     EATNSLNIAHKSQTHLLSQEAAGDAIELSLLMVHGQDHMMTALAFIDLAKELVTVYDK
                     MSAIQRGES"
     gene            complement(9287..9598)
                     /locus_tag="VHD02_001439"
     CDS             complement(9287..9598)
                     /locus_tag="VHD02_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ5498040.1"
                     /translation="MKSIMLVCNAGMSTSMLVTKMQKAAQARDLEVSIWAVPVSEADN
                     EVAANSIDVLLLGPQVKFLLKDFKDKFEPDIKVDAINMADYGLMNGEKVLETALAMME
                     E"
     gene            complement(9621..11615)
                     /locus_tag="VHD02_001440"
     CDS             complement(9621..11615)
                     /locus_tag="VHD02_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEQ5498041.1"
                     /translation="MLSKKELAIITFLIHHKEQFVSSATLAEVIGMSDRTVRKYLKEL
                     ISSLPEHGAHIISKQGRGYCLEIDHSMAFDIFWQESVTSKKRLADVTQVEESVDREDY
                     VLHKFFFEDAVQDFEELCQELYISRTTLKHVLAGIKERIIPYQLELEITHQHIQIRGK
                     EEDIRHFIMDYFFVTSFDNTLSTMVGNTFLEGINFAEIMIIVLDECRDAKLKLSDFVM
                     NNLVLHIALMVQRIRSGCPLELFSIPIAIRQSDEYQVALRILYRVEEVMGIRFPKEEA
                     NYIALHLKVKHSVGKHWQDDNTDEKLQDHLKACIAKISQLTGMTLETDTNLFQGLLAH
                     MMPLTTRLENHIQLTNPLTEEIKSQYPEIFTLTKQTFSDLLVCQKNDVSDDEWAYISL
                     HLMAAIERYSNRYKLRVLVVCATGYGSAMMLKNRLEKEFEGRLRIVDVISYYEITEER
                     LKTVDLIISSISLANLMFLTPVITVSVFLSNQDIETIRQFIGEQEGIKKEVSLPSQMS
                     LAKAEQLLTGVFSPNRFLYLDEKISKEDLLLRMIACLDEAGTETFVEDFYHQMVLREN
                     YSPVIYGEVLAFPHPANPMTYSEQVVVAICREPLEWDKAHQAVHFVFLLSPSKGHNHR
                     LKYVSPGLASFVNQVELQQALLEEPNYSKFMTVFTPLIHD"
     gene            complement(11721..12815)
                     /locus_tag="VHD02_001441"
     CDS             complement(11721..12815)
                     /locus_tag="VHD02_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEQ5498042.1"
                     /translation="MVDLGFSLYPERYDVTKSKAYIDLCHSYGAKRLFMSLLQLAPAD
                     HQMFHCYAELIAYANQLGIRVIADVSPSFISQAGWSDQLIERAHAFGLAGLRLDEALP
                     LAEIVTLTRNPFGLKIELNMSTDKQLLMSLLATDAEQSNIIGCHNFYPHEFTGLSWQH
                     FKDMSRFYHEHDIETAAFITAQSASEGPWLLAEGLPTVEDHRHLPIGLQVELMKAIGT
                     IDNILISNQFISEEELAACTQALARPVTTIKVRPIIDLTEVEEKIIGYPHCYRGDVSD
                     YVIRSTMPRLVYAQESIAPRDQSKEVKRGSIIIDNDRYHRYKGELQITLKNFTVSSKA
                     NVVAEVREDYLSLLDDLRPWQEFCLEIDPS"
     gene            complement(12824..14224)
                     /locus_tag="VHD02_001442"
     CDS             complement(12824..14224)
                     /locus_tag="VHD02_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEQ5498043.1"
                     /translation="MKQRKHRYQFPDGFLWGSSTSGPQSEGTVPGDGKGPSNWDYWFS
                     IESAKFHHQIGPEKTSTFYENYKGDIALLKETGHTIFRTSIQWSRLIPEGVGEVNPKA
                     VTFYREVFQDIIAQGIKLIVNLYHFDLPYALQEKGGWENKATVWAYETYAKTCFELFG
                     DLVNTWITFNEPIVPVECGYLGYYHYPCKVDAKAAVQVAYNTQLASSLAVKACHKLHP
                     DHKISIVLNMTPAYPRSDAPEDVKAARIAELFQTKSFLDPSVLGVYPAELVSILEEAD
                     LLPQYSADELEVIKNNTVDFLGVNYYQPLRVQAPSKSQQEGDPLILDIYFEPYDMPGK
                     KVNPHRGWEIYEPGLYDIALNLKEHYGNIEWLVTENGMGVEGEEAFLADGQIQDDYRI
                     TFIEDHLIQLHKALGEGANCKGYLLWTFIDCWSWLNAYKNRYGLVALDLESQKRTIKK
                     SGYWFKALSESNGFDK"
     gene            14449..15144
                     /locus_tag="VHD02_001443"
     CDS             14449..15144
                     /locus_tag="VHD02_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEQ5498044.1"
                     /translation="MAYLNYFTKTEWALWLSSVLAILISALLFGKQAPLALIASLIGV
                     TSLIFSAKANPIGQGLVIIFSIIYAYLSLRNSYYGELMTYLFMTLPMTIFSLFTWLNH
                     PFEGKKSQVTISRLTPTDRRCLFVFTILVTLIFYGILASIQTAYLLVSTLSIATSFSA
                     VYLSYKRSPYFALAYGLNDLVLILLWMHAAQTDASQYAVVICFATFLINDSYTFYSWL
                     MLQRYQEKKVKKG"
     gene            complement(15149..15307)
                     /locus_tag="VHD02_001444"
     CDS             complement(15149..15307)
                     /locus_tag="VHD02_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498045.1"
                     /translation="MGDKPISFKDKDGNFVSAADVWNAEKLEELFNLLNPNRRLRLER
                     EKLKKDEA"
     gene            complement(15376..16689)
                     /gene="obgE"
                     /locus_tag="VHD02_001445"
     CDS             complement(15376..16689)
                     /gene="obgE"
                     /locus_tag="VHD02_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEQ5498046.1"
                     /translation="MSMFLDTAKISVQAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFRVDEGLRTLMDFRYNRKFKAKSGEKGMTKGMHGRGAEDLIVFVPQGTTVRDAET
                     GKVITDLVEHGQEVVIAKGGRGGRGNIRFATPRNPAPEIAENGEPGEERQLELELKIL
                     ADVGLVGFPSVGKSTLLSVVSSAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYEDYVSINNELETYNLRLME
                     RPQIIVANKMDMPEAQENLKAFKKKLAAQYDEFDDLPMIFPISSLAHQGLENLLEATA
                     ELLAKTDEFLLYDEADLVDEEAYYGFAETEKDFEITRDDDATWVLSGEKLERLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDPVRIGKFEFEFVD"
     gene            complement(16746..16874)
                     /locus_tag="VHD02_001446"
     CDS             complement(16746..16874)
                     /locus_tag="VHD02_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEQ5498047.1"
                     /translation="MAFGENGPRKKTTFEKVTMFVVILMVLVTVGGLIASALSVLM"
BASE COUNT         5162 a         3650 c         3150 g         5188 t
ORIGIN      
        1 aaacctagtc cattaattta agttctaaaa tcaagtctcg catttgagct gccaattcga
       61 aatcaagtaa ttcagctgcc tcttgcattt gtttttgcag agcattgatc gcttcttttc
      121 gttctcctct tgacatactt tcataatcca tctcttcttt tgatatatca ttgtggcttg
      181 ttttacttat actaataagg cctctgatgt ctttttttat agtttgtgga acgatgccgt
      241 gcgctttgtt atatgctatt tgaatctcac gacgacgagc cgtttcgtca atagcccgct
      301 gcatagaatc tgtcatcttg tccgcataca taatgacatg accatctacg tttcgagctg
      361 ctctaccgat ggtctgaatc aagccgcgtt cattacggag gaaaccctct ttatcagcat
      421 ccaaaatggc tacaagacta acttctggca catcaatccc ttcacggagc agattgattc
      481 caatcaagac atcaaaaaca ccaagtcgta aatcacgaat aatttctgtc cgctctaagg
      541 ttttaatatc actgtgcata tacttgacct tgacacccat ctcttttaga taatcagtca
      601 aatcttctgc cattttttta gttaaggttg tgatgaacgt ccgttcatcg cgcgctacgc
      661 gctggttaat ttctcccaac aaatcatcca tttgccccat acttggcctt acatcaattt
      721 caggatcaag tagcccagtc ggacgaataa tttgctcaat aattgtatta gtctgtgaca
      781 tttcgtattc accaggcgtt gctgaaacat agacaatttg gtgcacatga ctttcaaatt
      841 cctcgcgtct taagggacga ttatcaagag ctgatggcaa tcgaaaacca tagtcaacta
      901 gcatttgttt tctggcttgg tcaccattat acatgccttt gatttgtccc attgtcatgt
      961 gactttcatc aatcataatt aaaaaatctt ctgggaagaa atctaacaag gtataaggag
     1021 gttctcctgg tgacctgcca tccatatgac gggaatagtt ttcgactccg ctagtatagc
     1081 ccatttcacg taacatttca atatcatatt ccgttctttg gcgtaatcgc tgtgcttcta
     1141 gtagtttacc ttcagattca aataactgta actgctctgc taactctgct tgaattttgg
     1201 ctattgattg ctccatatgc tcatcatttg ttacaaagtg agtagctgga aataaaacca
     1261 aatggtcaac ttctccaata gttttcccag ttaaactttc gatttcacat atacggtcaa
     1321 tctcatcccc gaaaaattcc acccgaaaag catgttcatc cctggatgct ggaaagacct
     1381 caacaacatc gccacgtaca cgaaaacagc cgcgttgaaa atcaatatca ttacgctcaa
     1441 attgaatatc tactaattga tttagcaaag tgtcacgtga tatctcctga cctggacgta
     1501 ggctgactgc tgaatctgcg tactcttttg gcgaacctag tccataaata caagatacag
     1561 aagctaccac tatgacatcg ttacgctcaa gtaaagaaga ggtggctgaa tggcgaagtt
     1621 tatcgatttc atcattgact gaactatctt tttcgatata ggtgtcgctt gatgggacat
     1681 atgcttcagg ttggtagtaa tcataataag aaacaaaata ttccacagca ttgtctggaa
     1741 aaaattcttt aaattcacca tataattgtc ctgctaaggt tttattgtga gctatcacta
     1801 aggtaggttt atttacttta ctaattactt ggctcatggt gtatgttttt cccgttcctg
     1861 tagcacctag gagaatctga gctttttctc cgccctcgat attatcaact aggctttcaa
     1921 tagcttgtgg ctgatcacca gacggtttat actttgattt tagtttaaat ggtttatcat
     1981 cacgtttatc aatcatttta taccctctaa tagtcttact ctcttataga ctattttaac
     2041 atagataagc cagaatcacc tccaataagc gtaaaaaaca tgtcatttac atgttatgtt
     2101 ttatgacata taaataacaa atttcttgct taatgttcaa tattttgtta taattaacca
     2161 tattttttag ttttataagg agattagtat gacgcacaaa ataaaagtat tgctgcttgc
     2221 gataatgtct atttttttga catgcaatat tgcaagtgct gaaactattg ctattgtttc
     2281 agatacagct tatgccccat ttgaatttaa agactcagat caaatttaca aaggaattga
     2341 cgttgatatt attaatgaag tagccaaacg tcaatcttgg gatttcagta tgagtttccc
     2401 gggttttgat gcagctgtaa atgctgttca atctggtcaa gcgagtgctc taatggccgg
     2461 tacaaccatt acgaatgctc gtaagaaagt ctttcatttc tcagagccat attacgatac
     2521 caaaattgtc attgcgacac gtaaagccaa tgccatcaaa aaatacagtg acttaaaagg
     2581 aaaaacggtc ggtgttaaaa atggaacagc ggctcaagcc tttttgaata actataaaaa
     2641 aaagtatgat tatactgtta aaacatttga cacaggtgat cttatgtata atagtttatc
     2701 tgctggttct attgccgctg ttatggatga tgaggcggtt atccaatacg caatcagcca
     2761 aaaccaagat attgctatta acatgaaagg agagcccatt ggaagctttg ggtttgctgt
     2821 caaaaaggga agcggatatg attatctagt taatgatttc aatacagctc ttaaagctat
     2881 gaaagctgat ggtacctacc aagctatcat gaccaagtgg ttaggcacag atgataaagc
     2941 taccaccagt caggcaacgg gaaatccatc tgccaaagct acacctataa aggacagtta
     3001 taaaattgtc tctgattcgt cttttgcacc ttttgaattt caaaatggta agggcaaata
     3061 cgttggtatt gacatagaat taatcaaagc tattgctaaa caacaaggtt tcaaaattga
     3121 aatcgctaat ccaggtttcg atgctgcctt aaatgctgtg caatctagcc aagcagatgg
     3181 ggtcattgct ggtgcaacta ttactgacgc tcgtaaagct atctttgatt tttctgatcc
     3241 ttattatact tctaatatca ttttagctgt taaagctgga aaaaacatca agaactatga
     3301 agacttagac agaaaaacag tcggtgctaa aaacggcact tcatcttact cttggttaaa
     3361 agaaaacgct cctaaatatg gttataatgt caaggcattt gatgatggtt ctagcatgta
     3421 tgatagctta aattcaggtt ctgtagatgc tatcatggat gatgaggcgg ttcttaaata
     3481 cgctatctct caaggtcgtc gctttgaaac acctcttgag ggcatttcta ctggtgaagt
     3541 tggttttgct gtcaagaaag gaactaatcc agaattaatc gaaatgttca acaatggctt
     3601 agctgctctc aaaaaatctg gtcagtatga tgacattata gataaatacc ttgactctaa
     3661 gaaagctgca actccttctg aaaaaggtgc tgatgagtct actatttcag gcctattatc
     3721 aaataactac aaacaactat tggcaggact tggaaccacg ctcagtttaa cccttatttc
     3781 atttgctatt gctataatta tcgggatcat ctttgggatg atggccgtgt caccaactaa
     3841 atcacttcga ctcatttcaa cggtctttgt ggacgttgtt cgagggattc ctttgatgat
     3901 tgtggctgcc ttcattttct ggggagtacc aaaccttatc gagagtatga ccggccacca
     3961 gtcaccgatt aatgatttct tagctgctac aattgcactg tcacttaatg gcggagccta
     4021 tattgctgaa attgttcgcg gtggtatcga agctgttcca gcagggcaaa tggaagctag
     4081 tcgaagtctt ggtttgtctt acggaaccac gatgagaaaa gtaattctcc cacaagctgt
     4141 gaaactaatg ttacctaact ttatcaatca gtttgttatt tcattgaagg atacaacaat
     4201 cgtctcagca attggtttag tggaactctt ccaaacaggt aaaatcatta ttgctagaaa
     4261 ttaccagtcg ttccgcatgt atgctatttt agcaattatt taccttatca tgattacact
     4321 cttaacaaga cttgcaaaac gtttagaaaa gaggcttaac taatgacaga attaaaaatt
     4381 gatgttcaag gtttacacaa atcttatggt caaaatgaag tcttaaaagg aattgatgcc
     4441 aaattctacg aaggtgatgt cgtttgtatc attggtcctt ctggttctgg gaaatcaacc
     4501 tttctacgta ccctcaacct tctagagacg attaccagtg ggaaggtcat ggtggacggt
     4561 tttgaattat cagatcctaa gactaatatt gacaaggcac gtgaaaatat cgggatggta
     4621 ttccaacact tcaacctttt ccctcacatg actgttcttg agaatattat ctttgctcct
     4681 gttgaattag gtaaagagtc taaagaagtt gctaaaaagc atggcatggc acttctagaa
     4741 aaagtcggac tttctgataa ggccgacgcc ttcccaggaa gtttatccgg cgggcaaaaa
     4801 cagcgtgtcg ccatcgccag aagtttagca atgaacccag atattatgct ctttgatgaa
     4861 ccaacttctg ctcttgaccc cgaaatggtt ggggacgttt tgaacgttat gaaagacttg
     4921 gcagaacaag gcatgaccat gttaattgtt actcacgaaa tgggatttgc tcgccaagta
     4981 gctaaccgtg ttatctttac agacggtggt caattcttgg aggatggtac tcctgaagag
     5041 atttttgatc atcctaagca ccctcgtctc attgaatttt tagacaaggt cttaaacgtc
     5101 taatagagca gacaccatac taacttccaa aaaataataa ggcaaggaaa caggtttcct
     5161 tgcctcagac tgaagacaaa gtccttagaa tcacttttct aggggctttt catgatactt
     5221 aggagtataa tataagaaaa aggacttgtg aggttctcct atgttacaca aagaaaaacc
     5281 tgactataac cgcaatcagt acggtttcta tacccttgac cagatagtgc ctgatgaaca
     5341 tttcctgcgc caagtggaag ctgtgattga cttcgacttc atctatgacc tagtagaaga
     5401 cacctattcg tctgataatg gtcgccctag ccttgatcct gtcatgttgg ttaaaatccc
     5461 tctgattcag tgcttttatg gcattcgttc catgcgtcag accatcaagg atattgaagt
     5521 gaatactgcc tatcgctggt ttttaggttt gactttggat aacaaggtgc cacatttcac
     5581 aacctatggc aaaaattatt cccgtcgctt tcaagaaaaa ggcttgattg agtctatttt
     5641 tacgcatatt cttgggctat gtatcaatac aggcctgatt gacccgatgg agattttcat
     5701 agatgggact catattaagg ctgcggctaa taatcgcaag ttcatcaacc gagaggtgga
     5761 aaaacaagcc aagttcatga gtgaacagct agaaatcgaa atcaatcggg atagggagaa
     5821 gcatgggaaa aagccgctag ggcccgtcgc taaaaaaatc tccacaaccg atccagaaag
     5881 tggctggttt cacaaaggtg atcataagga agtcttcgct tattctgctc aggtggcttg
     5941 tgacaagtat ggatgggcgc ttgcttatag tgttgaggct ggcaatatcc atgatagtca
     6001 ggcttttcct gcactttttg ccaagcttga acccttgaaa ccacagttta ttattgcgga
     6061 ttccggctat aacattccca gcattgccaa gtttctgatt gataaggaga tcacttctgt
     6121 ccttccgtac acacgaccaa gaggaaagaa agaccttcta cgtccgaaag aatttattta
     6181 tgatgagcac tttgattgtg tcatctgccc tgaacatcaa gccttgacct accgcactac
     6241 gaccagagaa ggctaccgag aatacaaaag tgaccctgta atttgtgcca attgtcccct
     6301 gttatctgta tgtacgacca gtaagaacca cctaaaagtt gtcacacggc atatttggaa
     6361 agactatttg gaacagtgcg aggacattcg tcatcaaagg gggatgaagg agctctacca
     6421 gcatcggaaa gagacgattg aacgactgtt tggcacagcc aaggaatacc acaatctccg
     6481 ctatacgaga gagaaaggca agtccaaaat ggaagataag gttggactga ctttggcatg
     6541 cttaaatctc aaaaaacggg tcaaaaggat ggtgggagag cctttttatt ttgttcagat
     6601 gaggtggttt aagcacggaa acagacattt ttatctaaaa acactaaaaa agacaaacac
     6661 caaatcgatg tttgtcttca atctgaggca aggaaacagg tttccttgcc ttttttggtt
     6721 actgttatta ttcaaaccag caaaataggg atactcttat gactctctcc ttcataagag
     6781 tgtcccctat gcttttttat gggcgtttat gtttagtgag gtaataacct aatcccgtaa
     6841 gagctagtcc cgtcactatg tgtgagtatt tgttaccaac catcggacta atggcaatga
     6901 gaaaaaagcc aacagccatt aaaaaagttg ctccattttt ttctgtcatg caaggtcact
     6961 cgctttttct ttattggcca aaatgacaaa tggtgcccag ataaggaagg caacaaagag
     7021 gttaaacagt gaaaccaaac ctgccataat attacctcca gtagctaggt aagcataaag
     7081 tccaacaggg gtaatccaag gtactgataa gaataccggt ggaatcaaac ctattgcagt
     7141 agcaaaataa gctatgctag cacaaattgg tggcacaatt agccatggaa tcacaaaggt
     7201 tggattcaaa acaattggca tcccaaatgt aattggttcg ttgatattga aaacacccat
     7261 tggtgcggac aatttggcaa tggttttatg ctcttctcgt tttgagaata tgaagatagc
     7321 aataatcaat gccaaggtta ctcctgaacc acccatttga gcataagcat caaaggaccc
     7381 gcgtgtccaa aggtatggta aattagcagc gctatgcgct gtattgtaag cagctgtatt
     7441 ttctgttaaa gctaccatat aaataccatc catcactggg gctaagacat tatgaccatg
     7501 caagccgaag aaccagagca gttgaactaa gaaggttaac aagataactg aaccaatacc
     7561 ctgtgataag cctagtaatg gtaattggat ataggttgaa atgacatcac ttagggaaga
     7621 acctgttaat gcaaagatga ggtaggcgaa aattgccgaa gcgtaaatcg caactgttcc
     7681 tggaataatg gcagcaaagg ctttattaac cgcaggtggt acattatctg gtaaggtaat
     7741 ggtaatatta cgtttggtca aagccgcata cacaaaactt gataaaaagc caataattaa
     7801 agctgtgaac aagcctgtag caccgacata ctttagagct attgctcccc attgactagt
     7861 ttcaatactt gaacctccac ccgcagtaac gactgaaagc cccatttctt ttaaattgct
     7921 aattaggtta gcagaggcat tttcaagtgg tgtcgaaatg gttaaagtct gaggtagggt
     7981 ggcaataaat gagccaaaag aaatcaatcc tcctgccaat ggattaatct tatacataac
     8041 agctaaattg taaccgaaag caaaggcaaa aaagagcgcc atgataccaa ttgtcccaaa
     8101 ataaacatag ccattgatgt cgataacagg ttgcatagct tttgcaaacc ctgtccatcc
     8161 catattgttt ggaatatcgc gtaaaaaaac gttcaataag acagcaactg aacctgccat
     8221 ggtaataggc atcattgaaa taaaggaatc acgaatagct actaaatgcc gttgtgaccc
     8281 aatttttcct gaaatatcca taaacttatc agaaaaatgc gacataaatc ttctcctctt
     8341 tttatttttt taatgtaaca aaggtaacct taaaagcagc ggtgtctgcc taacacaatt
     8401 atctcaaaat catccttgtt aaacgtgatg cttagagata accggtaatc gactcttatc
     8461 caaatgaacc cttctctctc atccaaggtc tgtgaataat cttcacaaag acgatggttg
     8521 ctgcaactgt ttagcatatt ggataagggc tgctaactga gcttgagatt ggcgttctag
     8581 gctttttttg aacagtttgc caacaataaa atgatttgct ttttgaaaaa tatctagagg
     8641 atgatagtca taagaatagg taattctagt ccgattctcc cccagagcct ctaatcgata
     8701 agccaattcc tttatcccat gattggaact aaaacaagca gtatataatt gcggagcttc
     8761 tagggccgta ataagcaccc tcactgtacg ctctttgtat ttaccataat gtttaacata
     8821 gtttatcccc gctttgatat catctcttga caactgtctc aaggtatttt gtcgatgatc
     8881 ttcttggagc gatatcagca tggcttcaaa acaatgatga acggaaacat ctccttctgc
     8941 tgaaatgatc atgactcacc cctctgtatt gctgacattt tgtcgtaaac ggtaaccaat
     9001 tccttggcta aatcaataaa ggctaaggca gtcatcatgt gatcttgccc atgcaccatc
     9061 aataaagaca attcaatcgc atcaccagct gcttcttggg agagcaaatg tgtctgcgat
     9121 ttatgggcaa tatttaacga gtttgtcgct tcttgaagta agtgctgagc ctcctcaaac
     9181 tgcccatctc gtgctgcatg aatagcctct acggccttgc ccttggcttc gcctccatac
     9241 atgattaagc ccataatagc ttctaagttg gatggttccg tcatgcctac tcctccatca
     9301 tcgctagggc tgtttcaagg actttttcac cgttcataag accataatca gccatgttaa
     9361 tggcatctac tttaatatct ggttcaaact tatctttaaa atcctttagt aagaatttga
     9421 cttgtggacc taacaagaga acgtcaatgc tattagcagc cacttcgttg tcagcttcac
     9481 tcacaggaac tgcccaaata gaaacttcaa ggtcacgcgc ttgggctgct ttttgcatct
     9541 tagtcaccaa catactagtt gacataccgg cattacaaac taacataata gatttcataa
     9601 ggtttctcct tttaatcgtt ttaatcatga ataagtgggg taaacactgt catgaatttg
     9661 gaataatttg gttcttctag taacgcttgc tgaagctcca cctgattgac aaaagatgcc
     9721 aaacctggtg atacatattt taacctatga ttatgccctt tagacgggga taatagaaag
     9781 acaaaatgaa cagcttgatg cgccttgtcc cactcaagag gctctcggca aatagccact
     9841 actacctgtt cagaataagt catgggatta gcaggatgcg gaaaggcaag cacttctcca
     9901 tagatgactg ggctgtaatt ttccctcagt accatttgat gatagaagtc ctctacaaac
     9961 gtttctgttc ccgcctcgtc aagacaagca atcatccgta aaagcaagtc ttcctttgag
    10021 attttctcgt ctaaataaag aaaacgatta gggctaaaaa cacctgttag aagctgctct
    10081 gcttttgcta aactcatctg tgacggtaaa ctgacctctt tcttgatacc ttcttgttct
    10141 ccaataaatt gtcgaatagt ttcaatatcc tgatttgaca aaaaaacact gacagtaatc
    10201 actggtgtta aaaacatcag attggcaagg ctgatagagg aaataatcaa atcaacggtt
    10261 ttcagtcgct cttcggttat ttcatagtaa ctgataacat cgacaatacg caaacgtcct
    10321 tcaaattcct tttccaagcg atttttcaac atcatggcac taccataacc tgtggcacaa
    10381 actactagaa ccctcaattt ataacggttt gaataacgct caatggccgc cattaaatgc
    10441 aaactaatat aagcccattc atcatctgag acatcattct tttgacatac aagcaaatcc
    10501 gaaaaggtct gcttggtaag ggtaaatatt tcaggatact ggcttttaat ttcctcagtt
    10561 aaaggattag tcagctgaat atggttttca agacgtgtgg tcaagggcat catgtgggct
    10621 aataaacctt gaaataaatt ggtatctgtt tctaaagtca tgcctgtcaa ttgactaatc
    10681 ttggcaatac atgcttttaa atggtcctgc aacttttcat cggtattatc atcttgccaa
    10741 tgcttaccaa cagaatgttt gacctttaga tgcagtgcaa tataattagc ctcctcttta
    10801 ggaaaacgta tccccatcac ttcttccact cgatacaaaa tgcgcagagc tacttgatac
    10861 tcatcagact gacgaatagc tattggaatg gaaaacagtt ctaatggaca ccccgatcga
    10921 atacgctgaa ccattagggc aatatgcaag actagattat tcatgacaaa atcagaaagt
    10981 ttcaacttag catcccgaca ttcatccaag acaataatca tgatttctgc aaaattaata
    11041 ccttcaagaa aggtattacc taccatggtg gacaaggtat tatcaaaact agtaacaaaa
    11101 aaatagtcca tgataaaatg cctaatgtcc tcttctttac ctcttatttg aatatgttgg
    11161 tgagttattt caagctctaa ctggtaaggt attatgcgct ccttaatgcc tgctaaaaca
    11221 tgttttaaag ttgtccgact gatatacaat tcttgacaaa gctcttcaaa atcctgaacg
    11281 gcatcttcaa aaaagaattt atgaagcaca taatcttctc gatcaaccga ttcttccact
    11341 tgagtcacgt ctgctaaacg ttttttagac gtcacacttt cttgccaaaa gatgtcaaat
    11401 gccatagaat ggtcaatttc taagcagtaa cctcgacctt gtttagaaat gatgtgagct
    11461 ccgtgttcag gaaggctact gatcagttct ttgaggtatt tcctaaccgt acggtcactc
    11521 ataccaataa cctcagccag agttgcacta gagacaaact gctctttgtg atggattaga
    11581 aatgttatga tggccagttc tttttttgat aacattagtc ctccttacta ctttagaaaa
    11641 cgctctctac cctcttttag tgtaccaaat atttcctgaa aaaggtgacc atcattttcc
    11701 gcattgattg tgaaaagaat ctatgatggg tcaatctcca aacaaaattc ctgccaaggt
    11761 cgcaaatcat ctaacaaact aagatagtcc tctctgacct cagcaaccac attagctttt
    11821 gaactaaccg taaaattttt caaagtaatt tgcagttctc ccttatatcg atgatagcgg
    11881 tcattgtcaa taataatgct acctcgtttg acctccttag attggtctct tggagcaatc
    11941 gattcctgag cataaactag acgtggcatg gttgatcgaa tcacataatc agatacatcg
    12001 ccacgataac aatgcggata gccaatgatt ttctcctcaa cttctgtcaa atcgatgata
    12061 ggcctaacct tgatcgtcgt gactggtctt gctaaagcct gagtacatgc tgccaattct
    12121 tcttccgaaa tgaattggtt agatatgagg atattatcaa tggttcctat cgctttcatt
    12181 aattctactt gcaggccaat gggcaagtgc ctatggtctt ccactgtcgg caagccttct
    12241 gccagtagcc aagggccttc agatgctgac tgagcggtaa taaaagcagc cgtttcaata
    12301 tcatgctcat gataaaaacg tgacatatcc ttaaaatgtt gccatgacaa accggtaaat
    12361 tcatgtggat aaaaattatg gcaaccaata atattgctct gttctgcatc tgtcgctagc
    12421 aatgacatca ataattgttt atcagtgctc atattaagtt caattttaag cccaaaggga
    12481 tttcttgtta aagttacaat ctctgctaga gggagagcct cgtctaagcg taagcccgct
    12541 aacccgaagg catgagctcg ttcaataagc tgatctgacc aaccagcttg gctaataaag
    12601 cttggactaa cgtcagcaat tactcgaatg cctaattgat ttgcataagc aatgagctct
    12661 gcgtagcaat gaaacatctg gtgatcagct ggtgccagct gtaagagact cataaacaag
    12721 cgcttggctc catagctatg acataagtca atataagctt tactcttggt aacatcatac
    12781 cgctcagggt atagagaaaa tcctaaatca accatattgc ctcctattta tcaaaaccat
    12841 ttgactcgct caacgcctta aaccagtaac ctgatttttt tatggttcgc ttttgactct
    12901 ctaaatccaa agctaccaaa ccatatcggt ttttataagc atttaaccaa gaccagcaat
    12961 caataaaagt ccacaagagg tagcctttgc agttggctcc ttctcccaaa gccttatgaa
    13021 gctggataag atgatcctcg ataaaggtga tgcggtagtc atcttgtatc tggccatctg
    13081 ctaaaaaagc ttcttcacct tcgacaccca taccattttc cgtgactagc cattcgatgt
    13141 taccatagtg ttcttttaaa ttaagggcaa tatcataaag tcctggttca taaatttccc
    13201 aaccccggtg aggattgact tttttaccag gcatatcata aggttcaaaa taaatatcta
    13261 agataagggg gtccccctct tgctgtgact tactaggtgc ctgaacccgc aaaggctgat
    13321 aataattaac tcccaaaaaa tcaacagtat tgtttttgat aacctctaat tcatcagcac
    13381 tatattgagg taataaatca gcttcctcta aaatggaaac taactctgct gggtaaacac
    13441 ccaaaactga tggatctagg aaggatttgg tttggaaaag ctctgcgatt ctagcagcct
    13501 tgacatcttc aggagcatca ctccgaggat aagcaggtgt catgttaagc acaatactaa
    13561 tcttgtggtc gggatgaagc ttatgacaag ctttaaccgc cagtgaactg gccagttgtg
    13621 tgttgtaagc cacttgaact gctgccttgg catctacttt acaagggtag tgataataac
    13681 ctaaatagcc acattcgact ggaacaatcg gttcattaaa ggtaatccaa gtgtttacta
    13741 aatcaccaaa aagctcaaaa caggtttttg cgtaggtttc ataagcccag acagtagctt
    13801 tgttttccca gccacctttt tcttgcaagg catagggaag gtcaaaatga tagaggttaa
    13861 caatcaattt aataccttga gcaataatgt cttgaaatac ttctcggtaa aatgtcaccg
    13921 cctttggatt aacctccccg actccttctg gaatcaatcg agaccactgg attgaggtcc
    13981 gaaaaatcgt gtggcctgtt tctttcaaaa gggcaatgtc acccttatag ttttcataaa
    14041 aggttgaggt tttttctgga ccaatttggt gatgaaactt agctgactca atactaaacc
    14101 agtaatccca gttgctaggt cctttgccat ctccaggaac cgttccttcg ctttgaggcc
    14161 cagatgtgga acttccccat aaaaagccat ctggaaattg ataacggtgt tttctttgtt
    14221 tcatatcctt tgtcctttct ttgctcactt ctctttttat tataagaaga aaaacaggtg
    14281 atgtaaacgc tttaaaaaat ctgttttttc cttatcatca cggaaaataa gtgatttaaa
    14341 gcttgacata ttttattatc ttaaagagcc tatctaacct gccctgctac ttcttatcaa
    14401 aaccaagccc ctattgacag atgccttagc ctatgtttga ataggggtat ggcttattta
    14461 aactacttta caaaaaccga gtgggcactt tggttaagct ctgtcttggc cattttaatt
    14521 agcgcccttt tattcggtaa gcaagctccc ttagctttaa tagcttcttt gattggtgtg
    14581 acctctctca tttttagtgc taaagctaat cctattggtc aagggctcgt cattattttt
    14641 tccattatct atgcctatct ttcattacga aacagttact atggagagtt aatgacctat
    14701 cttttcatga ccctccctat gactattttt tcacttttta cttggttaaa ccatcctttt
    14761 gaagggaaaa aatcacaagt gactatttca cgattaacac ccactgatcg aagatgtctt
    14821 ttcgttttta ctatactggt cactcttatt ttctacggca tcttggcttc tattcaaaca
    14881 gcctatttac ttgtctccac tttatcgatt gccacttctt tctcagccgt ttatctcagc
    14941 tataagcgaa gcccttattt tgctttggct tatggactca atgatctagt cctcattctc
    15001 ttatggatgc atgctgctca aacagatgcc agccaatatg ctgtggtcat ctgctttgcc
    15061 acctttctta ttaatgatag ttacactttt tatagttggc taatgctcca acgttaccaa
    15121 gaaaaaaagg tcaaaaaagg atgagggcct aagcctcatc ctttttcaat ttttcacgtt
    15181 ctaagcgcaa acgtctattg ggatttaaaa gattaaacaa ttcttctaat ttctcagcat
    15241 tccaaacatc tgctgccgat acaaaatttc catccttatc cttaaaggat atcggtttat
    15301 cacccataat cagaaacctc catcttaaag ataaaacaca aggttgctat ggcaatgcca
    15361 tttctaaaga ccacattaat ccacaaattc aaattcaaat ttgccaatac gaacaggatc
    15421 cccgtctttt gctccacgtt cacgaagggc ttcgtcaacc cccataccgc gcaattgacg
    15481 ggcaaatttc atgatggatt cgtcacgttc catattggtc atgacaaaga gacgttccaa
    15541 tttctcacca gacaagaccc aagtagcatc atcatctcgg gtaatctcaa agtctttttc
    15601 agtttctgca aagccatagt aagcttcctc gtccaccaga tcagcttcat catataataa
    15661 gaattcatcc gtcttagcaa gtaattctgc tgttgcttct aacaagttct ctaagccttg
    15721 atgtgctagg cttgaaatag ggaaaatcat tggtaggtca tcaaactcgt catattgggc
    15781 agccaatttt ttcttgaagg ctttcaagtt ttcttgagcc tctggcatgt ccattttatt
    15841 agccacaata atctgtgggc gctccatcaa tcgcaaatta taggtttcca gctcgttatt
    15901 aatagaaaca taatcttcgt aaggatcacg accctcacta gcagacatgt caatcacatg
    15961 aagaataacc cgtgtccgct cgatatgacg gaggaattga gttcctaaac caaccccttg
    16021 gctagcacct tcaatcaaac ctggcagatc tgccatagca aagctatcac ctgacttggt
    16081 acgaaccatt ccaagattag gaacaatagt ggtaaaatga taggcaccaa ttttcggttt
    16141 agctgatgac acaacactta acaaggttga tttcccaaca gatgggaatc cgaccaagcc
    16201 aacgtcagct aagattttga gttctaactc taactgacgt tcctcacctg gctcaccatt
    16261 ttcagcaatc tctggcgcag gattacgagg agttgcaaaa cgaatattcc cacggccacc
    16321 acgaccaccc ttagcaatga ccacttcttg accatgttct actaagtctg taattacttt
    16381 tccagtttca gcatcacgaa ccgttgtccc ttgaggaaca aagacaatca aatcttctgc
    16441 accacggccg tgcatgcctt tggtcatacc tttttcacca gatttcgcct tgaatttacg
    16501 attataccga aaatccatca aggtacgcaa accttcgtcc actcggaaga taactgagcc
    16561 acctttacca ccgtctcctc cccaaggacc gccatttggg acgtactttt cacgacgaaa
    16621 agctaccata ccatcgccac cacggcctgc ttggacactg atttttgccg tatctaaaaa
    16681 catactcata ctttttttat tctctaactt tcttttactt taaaaaaacg cctttcagcg
    16741 tttccttaca ttaatactga tagggcactc gcaataagcc cgccaacagt tacaagaacc
    16801 attaggataa caacaaacat tgttactttt tcaaaggttg tttttttgcg aggtccattt
    16861 tctccaaatg ccacgttaaa tacctcatct attgattttt cttcttatat attaacatga
    16921 aagatcgttt taggcaaata ccagatgaat atttacgcta tgtagactat tactgagtat
    16981 tgtatcttgt gaatttcata tgaatatgat acattagcat ttgcaaaata atgttacaga
    17041 taaaaacctc ctcgtctggt gaactgcacc ccaaaagtta gacacaaagt ctaacggttg
    17101 gggtgctatt tttttatgaa atttaatcaa gaaacgaaag ttaagattta
//
LOCUS       DAUDYX010000044        16559 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.044, whole genome shotgun sequence.
ACCESSION   DAUDYX010000044 DAUDYX010000000
VERSION     DAUDYX010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16559
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.044"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            82..258
                     /locus_tag="VHD02_001447"
     CDS             82..258
                     /locus_tag="VHD02_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEQ5498048.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
     gene            complement(386..748)
                     /gene="mscL"
                     /locus_tag="VHD02_001448"
     CDS             complement(386..748)
                     /gene="mscL"
                     /locus_tag="VHD02_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEQ5498049.1"
                     /translation="MVKELKAFLFRGNIIELAVAVIIGGAFGAIVTSFVNDIITPLIL
                     NPALKAANVENITQLSWNGVKYGSFLGAVINFLIIGTSLFFVVKAAEKAMPKKEKEAA
                     APTQEELLTEIRDLLAQK"
     gene            878..2692
                     /gene="dnaG"
                     /locus_tag="VHD02_001449"
     CDS             878..2692
                     /gene="dnaG"
                     /locus_tag="VHD02_001449"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEQ5498050.1"
                     /translation="MGFLWGGDDLAIDKEMISQVKNSVNIVDVIGEVVKLSRSGRHYL
                     GLCPFHKEKTPSFNVVEDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQIIADKTGM
                     SLNIPPSQAVLASQHKHPNHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLDDQ
                     LIEHFNIGLAPDESDYLYQALSKKYEEGQLVASGLFNLSDQSNTIYDAFRNRIMFPLS
                     DDRGHIIAFSGRIWTAADMEKRQAKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVF
                     LMEGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAK
                     SLELLKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSRISSVEFFIDYLKPTNVD
                     NLQSQIVYVEKMAPLIAQSPSITAQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQK
                     RQGQIAQAVSNLVTLPIPKSLTAIAKTESHLMHRLLHHDYLLNEFRHRDDFYFDTSTL
                     EILYQRLKQQGYITSYDLSEMSEEINRAYYNVLEENLPKEVALGEIDDILSKRAKLLA
                     ERDLHKQGKKVRESSNKGDHQAALEVLEHFIAQKRKME"
     gene            2701..3810
                     /gene="rpoD"
                     /locus_tag="VHD02_001450"
     CDS             2701..3810
                     /gene="rpoD"
                     /locus_tag="VHD02_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEQ5498051.1"
                     /translation="MTKQKEITTFNVQVAEFIRHHKKEGTAIDDDVTEKLVIPFALDA
                     DQIDDLLERLTDGGISITDKEGNPSSKYIVEEPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTSEEEKELAVAVAKGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMEMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLRDFIED"
     gene            4046..4384
                     /locus_tag="VHD02_001451"
     CDS             4046..4384
                     /locus_tag="VHD02_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEQ5498052.1"
                     /translation="MSDTPKYTQDQVIAIKNRILEALETVIDPELGIDVVNLGLIYEI
                     RFNDNGYTEIDMTLTTMGCPLADLLTDYIHDALQDVPEVTKTEVKLVWYPAWTVDKMS
                     RYARIALGIR"
     gene            4522..5376
                     /gene="rfbD"
                     /locus_tag="VHD02_001452"
     CDS             4522..5376
                     /gene="rfbD"
                     /locus_tag="VHD02_001452"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEQ5498053.1"
                     /translation="MILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNEDKVEA
                     VFAQVKPTLVYHCAAYTAVDAAEDEGKALNEAINVTGSENIAKACGKYGATLVYISTD
                     YVFDGNKPVGQEWVETDRPDPKTEYGRTKRLGELAVERYAEHFYIIRTAWVFGNYGKN
                     FVFTMEQLAENHSRLTVVNDQHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDT
                     TWYDFAKEILKDKAVEVVPVDSSAFPAKAKRPLNSTMNLDKAKATGFVIPTWQEALKA
                     FYQQGLKK"
     gene            5495..6649
                     /locus_tag="VHD02_001453"
     CDS             5495..6649
                     /locus_tag="VHD02_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEQ5498054.1"
                     /translation="MQDVFIIGSRGLPAKYGGFETFVEELISHQSSKNIRYHVACLSD
                     TKHKVHFDYKGADCFYLNPPKLGPARVIAYDMMAITYALSYSDQHQIQNPIFYVLGNT
                     VGAFIAPFVKQIHNRGGRFFINPDGLEWKRSKWSKPVQAYLKFSEKQMTRQADLVISD
                     NIGIDRYLKQVYPWSKTCFIAYGTQTQPSRLTTADSKVRAYFQTFDIREKDYYLILGR
                     FVPENNYETAIKEFMASSTKRDLVIICNHEDNAYFKQLLAETECDKDPRIKFVGTLYD
                     KELLAYIREQAYAYIHGHEVGGTNPGLLEALAHTNLNLVLGVDFNQSVAKSAALYWSK
                     QKGQLAELINQVDAGFDSDRLGKEAKAIIQEHYTWEKIVGEYEALFLNEH"
     gene            6639..7571
                     /locus_tag="VHD02_001454"
     CDS             6639..7571
                     /locus_tag="VHD02_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEQ5498055.1"
                     /translation="MNINILLSTYNGERFLAEQIQSIQRQTVNDWTLLIRDDGSTDGT
                     QDIIRTFVKEDKRIQWINEGQTENLGVIKNFYTLLKHQKADVYFFSDQDDIWLDNKLE
                     VTLLEAQKHEMTAPLLVYTDLKVVTQHLAICHDSMIKTQSGHANTSLLQELTENTVTG
                     GTMMITHALAEEWTTCDGLLMHDWYLALLASATGKLVYLDIPTELYRQHDANVLGART
                     WSKRMKNWLTPHHLVNKYWWLITSSQKQAQLLLDLPLKPNDHELVTAYVSLLDMPFTK
                     RLATLKRYGFRKNRIFHTFIFRSLVVTLFGYRRK"
     gene            7574..8377
                     /locus_tag="VHD02_001455"
     CDS             7574..8377
                     /locus_tag="VHD02_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ5498056.1"
                     /translation="MNFLTKKNRILLREMVKTDFKLRYQGSAIGYLWSILKPLMMFTI
                     MYLVFIRFLRLGGNVPHFPVALLLANVIWSFFSEATSMGMVSIVSRGDLLRKLNFSKH
                     IIVFSAVLGALINFLINLVVVLIFALINGVTISGYAYLSLFLFIELVVLVLGIALLLS
                     NVFVYYRDLAQVWEVLLQAGMYATPIIYPITFVLDSHPLAAKLLMLNPVAQMIQDFRY
                     LLIDRANVTIWQMSTNWFYVVIPYLVPFVILFIGIFVFKKNADRFAEII"
     gene            8377..9582
                     /locus_tag="VHD02_001456"
     CDS             8377..9582
                     /locus_tag="VHD02_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5498057.1"
                     /translation="MINREIAVKVQHVSKTFKLPTEATKSFRTTLVNRLRGIKGFTEQ
                     HVLKDINFDVYKGDFFGIVGRNGSGKSTLLKIISQIYVPEKGQVTVDGKMVSFIELGV
                     GFNPELTGRENVYMNGAMLGFTKDEVDDMYNDIVDFAELHHFMNQKLKNYSSGMQVRL
                     AFSVAIKAQGDVLILDEVLAVGDEAFQRKCNDYFMERKDSGKTTILVTHDMGAVKKYC
                     NRAVLIEDGLVKAYGEPFDVANQYSVDNTETAEDAMNAEKISVSDIAKDLKVSLISNP
                     RITPNDTITFEVSYEVLKDDFTYIAFSLTDIDRNIWVYNDNSLDYPTQGIGRKCISYQ
                     CQLSQLNDIKLKLEVTVRDKNGQMLLFSTAEQSPQIVIQRNDIDQDDLSALDSASGLY
                     QRNGKWVFH"
     gene            9607..10614
                     /locus_tag="VHD02_001457"
     CDS             9607..10614
                     /locus_tag="VHD02_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEQ5498058.1"
                     /translation="MYKVSIICTNYNKAPWISDALDSFLSQVTDFEVEIIVIDDASTD
                     DSREILKSYQKKSSGKIKLLFNETNIGITKTWIKACLYAKGKYIARCDGDDYWTDSFK
                     LQKQVDVLEASKRSRWCNTDFDFVNSQGDLLYANAFSSGHTPLTDTYEKVLALKGMTM
                     ASTWLVDADLMRQVNQKINVDTPDDTFDIQLELFQLTQLTYIKDSTTVYRMTTDSDSR
                     PTDTPKMIHRIQKLLDTQLNYLKKYNQVDTKEMSELLLQQDAKQEIRIHELSCFIQEL
                     QQTIVDKTKQQETREVELQNVIEEQKNQLSELRQQYHAIINSRQWKYTSKLIAFIRRK
                     K"
     gene            10611..12356
                     /locus_tag="VHD02_001458"
     CDS             10611..12356
                     /locus_tag="VHD02_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-Rha alpha-1,3-L-rhamnosyltransferase"
                     /protein_id="HEQ5498059.1"
                     /translation="MNRILLYVHFNKYNKISAHVYYQLEQMRSLFSKIVFISNSKVSH
                     EDLKRLKNHCLIDEFLQRKNKGFDFSAWHDGLITMGFDKLEEFDSLTIMNDTCFGPIW
                     EMAPYFENFEEKETVDFWGITNNRGTKAFKEHVQSYFMTFKNQVIQNKVFQQFWQSII
                     EYENVQEVIQHYETQLTSILLNEGFSYQTVFDTRKAESSFMPHPDFSYYNPTAILKHH
                     VPFIKVKAIDANQHIAPYLLNLIRETTNYPIDLIVSHMSQISLPDAKYLLSQKYLNCQ
                     RLAKQTCQKVAVHLHVFYVDLLDEFLTAFEDWNFHYDLFITTDSDIKRKEIKEILQRK
                     GKTADIRVTGNRGRDIYPMLLLKDKLSQYDYIGHFHTKKSKEADFWAGESWRKELIDM
                     LVKPADSILSAFETNDIGIIIADIPSFFRFNKIVNAWNEHLIAQEMMSLWRKMDVKKQ
                     IDFQAMDTFVMSYGTFVWFKYDALKSLFDLELTQNDIPSEPLPQNSILHAIERLLVYI
                     AWGNSYDFRIVKNPYELTPFIDNKLLNLREDEGAHTYVNFNQMGGIKGALKYIIVGPA
                     KAMKYIFLRLMEKLK"
     gene            12353..14827
                     /locus_tag="VHD02_001459"
     CDS             12353..14827
                     /locus_tag="VHD02_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEQ5498060.1"
                     /translation="MIKDTFLKTNWLNISHHITLLVFGFYFSFYSLAKELVSSTAQPV
                     NYYTHLLNVSFVGYIISLIGLSYYLSRQVSRQLFLKTSFIVISYLIVSYWVQITQHLN
                     DKRFDIWSLTKNQFYQFQALPSLLIILVMATLIKILVAYFAIEKDRFGLLGYQGNTFS
                     VALILAVVPINDIHLLKLISSRFSELVRAGNSQIALLKISGLLIVLLVIFATIIYVVL
                     NALKHLKSNKLSFSVAATTSLFLALVFNYTFQYGVKGDEALLGYYVFPGATLFQIVAI
                     TLVAILAYVITNRYWPTTFFLLILGTIISVVNDLKESMRSEPLLVTDFVWLQELGLVT
                     SFVKKSVIVEMVVGLAICIVVAWYLHGRVLAGKLFMSPVKRASAVLGLFIVSCSMLIP
                     FSYEKEGKILSGLPIISALNNDNDINWLGFSTNARYKSLAYVWTRQVTKKIMEKPTNY
                     SQETIASIAQKYQKLAEDINKDRKNNIADQTVIYLLSESLSDPDRVSNVTVSHDVLPN
                     IKAIKNSTTAGLMQSDSYGGGTANMEFQTLTSLPFYNFSSSVSVLYSEVFPKMAKPHT
                     ISEFYQGKNRIAMHPASANNFNRKTVYSNLGFSKFLALSGSKDKFKNIENVGLLTSDK
                     TVYNNILSLINPSESQFFSVITMQNHIPWSSDYPEEIVAEGKNFTEEENHNLTSYARL
                     LSFTDKETRAFLEKLTQINKPITVVFYGDHLPGLYPDSAFNKHIENKYLTDYFIWSNG
                     TNEKKNHPLINSSDFTAALFEHTDSKVSPYYALLTEVLNKASVDKSPDSPEVKAIQND
                     LKNVQYDVTIGKGYLLKHKTFFKISR"
     gene            15006..15701
                     /locus_tag="VHD02_001460"
     CDS             15006..15701
                     /locus_tag="VHD02_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEQ5498061.1"
                     /translation="MKKLIIIPAYNESSNIVNTIRTIESDASDFDYIIIDDCSTDNTL
                     AICQKQGFNVISLPINLGIGGAVQTGYRYAQRCGYDVAVQVDGDGQHNPCYLEKMVEV
                     LVQSSVNMVIGSRFITKEGFQSSFARRIGIKYFTWLIALLTGKKITDATSGLRLIDRS
                     LIERFANHYPDDYPEPETVVDVLVSHFKVKEIPVVMNERQGGVSSISLTKSVYYMIKV
                     TLAILVVRLKGNR"
     gene            15703..16044
                     /locus_tag="VHD02_001461"
     CDS             15703..16044
                     /locus_tag="VHD02_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEQ5498062.1"
                     /translation="MTVTASLFISFIVLTFVFFLINLIKKDKLAIKYSLLWFILALLI
                     LLFTWLPNILNKMSHFLGIHSPTNMLFFLGFCLSLAIIFSLTNNISLQNDKVKRLTQE
                     VALMKKEKTND"
     gene            16037..>16559
                     /locus_tag="VHD02_001462"
     CDS             16037..>16559
                     /locus_tag="VHD02_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEQ5498063.1"
                     /translation="MINPSKQNQTIFLWNMLGSLSTAVISVILLMVVTRLLTSADSDI
                     YAFAYSFANMMVVVGLFQVRNYQATDINEKYSFSQYLVARLMTCLLMLAITVIYLTLT
                     KTDSYKSTIVFLVCFYRSTDAFSDLYQGMFQQHERLDIAGKSLAYRNTLIFMVYTAII
                     LYSKNLTLALVAVC"
BASE COUNT         5490 a         2673 c         3141 g         5255 t
ORIGIN      
        1 agaggtttga cttcttttaa aaaataatgt aatataattg ggaacgtgaa aatgttacaa
       61 tatcttgagg aggtgaaaca catgtcaaaa acagtagtac gtaagaatga atctcttgac
      121 gatgctcttc gtcgcttcaa acgttctgtt actaaagctg gtactcttca agaatcacgt
      181 aaacgtgaat tctacgaaaa accttctgta aaacgtaaac gtaaatcaga agcagctcgc
      241 aaacgtaaaa aattctaatg gtaaaaaata aaaacagttc tcacaattag aactgttttt
      301 attttttatc cagaagaagc aagggagctt ttttgatatc aaaaaaagca gttagacatg
      361 cctaactact ttatcaaaaa tcgctttatt tttgagccaa caaatcacgg atttctgtca
      421 ataattcttc ttgagtagga gctgcagctt ctttttcttt tttaggcata gctttttcag
      481 cggcttttac aacaaagaaa agactggtac cgatgataag aaaattaata acggctccta
      541 aaaagctacc atatttaaca ccattccatg acaattgagt gatgttttca acatttgcag
      601 ccttcagagc tgggtttaaa ataagcggtg tgataatatc gttcacaaaa gaagtgacaa
      661 tagcaccaaa agctcccccg atgataactg ctacagcaag ttcaataatg tttcctctaa
      721 acaaaaatgc ttttaattct ttaaccatta aatattcccc cttttaatat ctctaagtta
      781 agcatagcat aattagtaaa caaaatccaa tggaatcact tcagttttta aatttacaaa
      841 aaagagtttc ggtggtataa taaaacgtaa aactaaaatg ggatttttat ggggaggtga
      901 cgatttggca attgacaaag aaatgatttc ccaagtaaaa aatagcgtta atattgtcga
      961 tgtcattgga gaagtggtca aactttcccg atcagggcgg cattatctcg ggctttgccc
     1021 atttcataag gaaaagacac cctctttcaa tgttgttgaa gacagacaat tttttcactg
     1081 ctttggctgt ggaaaatcag gggatgtttt taaatttatt gaggaatacc gccaagtccc
     1141 cttcttagaa agtgttcaga ttattgccga taagactggt atgtcgctta atataccgcc
     1201 aagtcaggca gtacttgcta gccaacacaa gcaccctaat cacgctttga tgacacttca
     1261 tgaggatgct gctaaatttt accatgcagt tttgatgacc actaccattg gtcaagaagc
     1321 taggaagtac ctttaccaga gaggcttgga tgaccaatta attgagcatt tcaatattgg
     1381 tttagcccca gatgagtcag attatcttta tcaagctctt tctaaaaaat acgaggaagg
     1441 tcaattggtt gcttcaggat tgtttaactt gtccgatcaa tccaatacca tttacgacgc
     1501 ctttcgaaat cgtatcatgt ttcccttatc agatgaccga gggcatatta ttgccttttc
     1561 aggacgtatc tggacggcag ctgatatgga aaagagacag gccaagtata aaaattcaag
     1621 aggaacagtt ctttttaaca aatcttatga attgtatcat ctggacaaag caaggcctgt
     1681 tattgccaaa acccatgaag tgtttctaat ggaagggttt atggacgtga ttgccgctta
     1741 ccgttccggc tatgaaaatg ctgttgcttc aatggggacg gcattgactc aagaacatgt
     1801 caatcacctt aagcaagtca ctaaaaaagt tgttttgatt tatgatggtg acgatgctgg
     1861 acaacatgct attgcaaaat cactagaatt gcttaaagat tttgttgtcg aaattgtcag
     1921 aatccccaat aaaatggatc ctgacgaatt tgtacaacgg cattccccag aagcatttgc
     1981 agatttgctt aagcagtcac ggatcagtag tgttgaattt tttattgatt acctaaaacc
     2041 tactaatgta gacaatctgc aatcacaaat tgtttatgtg gagaaaatgg caccattgat
     2101 tgctcaatca ccatccatca cagctcaaca ttcgtatatt aacaagattg ctgatttgtt
     2161 gccaaacttt gactattttc aagtagaaca atcagtaaat gcattaagga ttcaagatag
     2221 gcaaaagcgt caaggtcaaa tagctcaagc cgtcagcaat cttgtgacct taccaatacc
     2281 aaaaagtttg acagctattg ctaagacaga aagtcatctc atgcatcggc tcttacatca
     2341 tgactattta ttaaatgaat ttcgacatcg tgatgatttt tattttgata cctctacctt
     2401 agaaatactt tatcaacggc tgaagcaaca aggatacatt acatcttatg atttgtcaga
     2461 gatgtcagag gaaattaacc gtgcttatta caatgtttta gaagaaaacc ttcccaaaga
     2521 agtagctctt ggtgagattg atgatatttt atccaaacgt gccaaacttt tagcagagcg
     2581 cgatcttcac aaacaaggga aaaaagttag agaatctagt aacaaaggcg atcatcaagc
     2641 ggctctagaa gtactagaac attttattgc gcagaaacga aaaatggaat agaggaaatt
     2701 atgacaaaac aaaaagaaat aacaactttt aacgttcaag ttgcggagtt tattcgccat
     2761 cacaaaaaag aaggaacagc gattgatgat gacgtgaccg aaaaacttgt cattccattt
     2821 gctttggatg ctgatcagat tgatgattta cttgagcggt taacagatgg tggtatctcg
     2881 attacggata aagaaggtaa cccatcatca aaatacattg ttgaagaacc aaaaccagaa
     2941 gaattaactg atgaagaatt aattggtagt aattcagcta aagtaaacga ccctgtccgc
     3001 atgtacctca aagaaattgg tgtggtaccg cttttaacaa gtgaagagga aaaagaattg
     3061 gctgttgcag ttgccaaggg agatttaatg gctaaacaac gtttagcgga agcaaacctt
     3121 cgtcttgttg tctcaattgc aaaacgttat gttggacgtg gaatgcaatt tcttgattta
     3181 attcaagaag gtaacatggg actcatgaaa gctgttgata aatttgatta ttcaaaagga
     3241 ttcaaattct caacttatgc aacatggtgg attcgtcaag ctataacacg cgcaattgca
     3301 gatcaagcac gtacgattcg tatccctgtt catatggttg aaacgattaa taaattagtc
     3361 cgtgaacaac gtaaccttct tcaagaactt ggtcaagatc caacgccaga acaaatcgca
     3421 gaacgtatgg aaatgactcc agacaaggtt cgtgaaattc taaaaattgc tcaagaacct
     3481 gtttctttag aaacaccaat tggggaagaa gatgatagtc acttaggtga ttttatcgaa
     3541 gatgaagtta ttgaaaatcc agtagattat acgacgcgtg ttgttcttcg tgagcaattg
     3601 gatgaagttt tagatacgct tactgaccgt gaagagaacg ttcttcgcct acgctttggc
     3661 cttgacgatg gtaaaatgcg tacgttagag gacgtaggta aagtctttaa tgttactcgt
     3721 gagcgcattc gacagattga agctaaagcc cttagaaaac ttcgccatcc tagtcgtagt
     3781 aaacaattaa gagactttat agaggactaa aatcgtccag tggacgattt taggtcgttc
     3841 ctttgaaata agaaaggagc gaaaatccta agtggcactt atcagcggta cacctacaaa
     3901 ttagaaatcg gaccgaatgt ggtgggtatt tttaaggttg gacttgatat cttaaacaag
     3961 cccagagggc aattcctcta aataggcaaa gctttagtgg tttttttact tatctacgac
     4021 atattcaaaa aatgaaagga aagccatgtc tgatacacca aaatatacgc aagatcaggt
     4081 gatagctatc aagaatagaa ttcttgaagc tttagaaact gttattgatc ccgagctagg
     4141 tattgatgtt gttaatctag gtttgattta tgaaattcgg ttcaatgata acggttatac
     4201 agagattgat atgacactca caacaatggg ttgtcctttg gcagacttgt tgacagatta
     4261 catccatgat gccctgcaag atgtgccaga agtcactaag actgaggtca aattagtctg
     4321 gtaccctgct tggacggttg ataaaatgag ccgctatgct aggattgcgc tgggtattcg
     4381 ctaatcattg atagtaaagg gatgaaatat tccctttttt gatgtagaat aaaaggaaat
     4441 acaggtgaaa gcctttggtt aatgttagag aacactaaga tactgagaaa agtgttctac
     4501 ctatggcaaa aaggagaact gatgatttta attacaggaa gcaatggtca attagggaca
     4561 gaacttcgat acttattaga tgaacgaggt gttgattatg tagccgttga tgtcgcagaa
     4621 atggacatca caaatgaaga caaagtcgaa gctgtttttg cgcaggtcaa gccaacattg
     4681 gtctatcatt gtgctgctta tacggcagtt gatgcagcag aagacgaagg aaaagccttg
     4741 aatgaggcta ttaatgtaac aggctcagaa aatattgcca aggcttgtgg aaaatatggg
     4801 gctacccttg tttacatttc aacagactac gtatttgatg gcaataaacc agtcggtcaa
     4861 gaatgggtag aaacagatcg tccagaccct aagacagaat atggccgtac aaaacgttta
     4921 ggagaactag ctgttgaacg atacgcagag catttttata ttatccgtac tgcttgggta
     4981 tttggaaatt atggtaaaaa ctttgtcttt accatggagc agttagcgga aaatcactcc
     5041 cgtttgactg ttgtcaatga ccaacacggt cggccaacgt ggacaagaac cttggcagaa
     5101 ttcatgtgtt atttgacaga gaatcaaaaa gcctttggtt actatcactt gtcaaatgat
     5161 gctaaggaag acaccacttg gtatgatttt gccaaagaaa tcttaaaaga caaggctgtt
     5221 gaagtggtgc cagttgattc atcagctttc ccagcaaaag ccaaacgtcc tttaaattca
     5281 accatgaatc ttgataaggc aaaagcaaca gggtttgtta ttccaacatg gcaagaagcc
     5341 ttgaaggcat tttaccaaca aggactgaaa aagtaagtat tctaaaaaga gttggctttc
     5401 taaggccaac tctttttcat gggtttaagg tcgcattagc cttctaattg tggtataata
     5461 tcactgatta tattttagaa tggggtagta tgcaatgcag gatgttttta tcattggtag
     5521 cagagggtta ccagcgaaat acggtggctt tgaaaccttt gtcgaagagt tgattagtca
     5581 tcaatcatct aaaaacattc gttatcacgt tgcttgttta agcgatacga agcataaggt
     5641 tcattttgat tataaggggg cagattgctt ttaccttaat cccccaaaac ttggtcctgc
     5701 tagagttatt gcttatgata tgatggcaat tacctatgcc ctgtcttata gcgaccaaca
     5761 ccagattcag aaccctattt tttatgtgtt gggcaatact gtaggcgctt ttattgcgcc
     5821 atttgtgaag cagattcaca atcgaggtgg acgctttttt atcaatccag acgggttaga
     5881 gtggaaacgg tctaaatggt caaaaccagt gcaggcctac ctgaagtttt ccgaaaagca
     5941 aatgactaga caggctgatt tggtcatttc agataatatt ggcattgacc gctaccttaa
     6001 acaggtttat ccttggtcta aaacatgttt tattgcttat ggaacgcaga cacaaccttc
     6061 acgtttgaca acagcagata gcaaggtcag agcttatttt caaacctttg atattagaga
     6121 aaaggattac tatctcattt taggacgctt tgttcctgaa aataattacg aaactgctat
     6181 taaagaattt atggcttcct caactaagcg tgatttagtg attatttgta atcatgaaga
     6241 caatgcttac tttaagcaat tacttgctga gacagagtgt gataaagacc cacgcattaa
     6301 atttgttggt accctttatg acaaagaact attagcctac attcgagaac aggcttatgc
     6361 ctatatccat ggtcatgagg taggagggac taatccaggc cttctagaag cactagccca
     6421 taccaatctt aatttggttt taggagttga ttttaaccag tcggtggcta agtctgctgc
     6481 gctgtattgg tccaagcaaa aagggcaatt agcagagctg attaaccaag ttgatgcagg
     6541 ttttgatagt gatcgtctgg gcaaagaagc caaagctatt attcaagaac attacacttg
     6601 ggaaaaaatt gtaggggagt acgaggcttt atttttaaat gaacattaat attttactat
     6661 ccacctacaa tggagaacgc ttcttagcag aacaaattca aagtatccaa aggcaaacag
     6721 ttaacgactg gaccttattg attagagatg atggatcaac tgatggcacc caggatatta
     6781 ttcgcacatt tgtcaaggaa gacaaacgaa ttcagtggat caatgagggg cagacagaaa
     6841 atctgggagt tattaagaat ttttacaccc tcttaaagca ccaaaaagca gatgtctatt
     6901 ttttcagtga ccaagatgat atatggttag acaataaact ggaagtgacc ttattggagg
     6961 ctcaaaaaca tgaaatgaca gctcctttat tggtttatac agacctcaaa gtggtaaccc
     7021 aacatttagc tatttgccat gacagtatga ttaagaccca atctggtcat gcaaacacaa
     7081 gcctacttca agaattaacc gaaaataccg taacaggcgg cacaatgatg attactcatg
     7141 ctctggctga ggaatggacc acttgtgacg gtttattgat gcatgactgg tatttagctt
     7201 tactagcttc tgctactggg aaattagttt accttgatat tccaacagaa ttataccgtc
     7261 agcacgatgc taacgtgttg ggagctagaa cttggtctaa acgcatgaaa aattggttaa
     7321 ccccccatca tttagtcaac aaatattggt ggttgattac ttcaagccaa aaacaggctc
     7381 aattactatt agatttacca ttaaaaccaa atgaccatga attggtaacg gcctatgttt
     7441 ctttattgga tatgcctttt accaaacgct tagctacctt gaaaaggtac ggttttagaa
     7501 aaaatcgcat ctttcacacc tttatttttc gtagtttagt tgttacctta tttggttaca
     7561 ggagaaagta agaatgaatt ttttaacaaa gaaaaaccgt attttactga gagaaatggt
     7621 aaaaacggat tttaaattac gctatcaagg gagcgcaatt ggctatctat ggtctatctt
     7681 aaaaccgctt atgatgttta cgattatgta cttggtattt attcgttttc ttcgcctggg
     7741 tggaaatgta cctcattttc cagtagcgct tttattggca aatgtcatct ggtctttttt
     7801 ttcagaagca actagcatgg gaatggtatc tattgtatct cggggagact tgttgcgaaa
     7861 attgaacttt tctaagcaca tcattgtttt ttcggcagtg ttaggagctt taattaattt
     7921 tcttattaat ttggttgttg ttttaatttt tgcgttgata aatggtgtga ctatatcagg
     7981 gtatgcttat ctctctcttt ttctttttat agaattagtt gttttagtgc ttggaattgc
     8041 attgttattg tcgaatgtct ttgtttatta tcgtgactta gctcaagtct gggaagtact
     8101 attacaagca ggtatgtatg ccactccaat catttatccg atcacttttg ttttagatag
     8161 ccaccctttg gcggcaaagt tgttgatgct aaatccagta gcacaaatga ttcaagattt
     8221 tcgttattta ttgattgaca gagccaacgt aacgatttgg cagatgtcaa ccaattggtt
     8281 ttacgttgtt attccatatt tagtaccatt tgttatatta tttattggca tctttgtctt
     8341 taagaaaaat gccgatagat ttgcggagat tatttaatga ttaatagaga aattgcagta
     8401 aaagtacagc atgttagtaa aacatttaaa ctgcctacag aagcaactaa aagttttcgc
     8461 acgactctgg ttaatcgcct aagagggatt aaaggattta ctgaacaaca tgtgctaaaa
     8521 gatattaact ttgatgttta taaaggcgat ttttttggaa ttgttgggcg taacggctcg
     8581 ggaaaatcaa cgctccttaa aattatttcc cagatctatg ttccagaaaa aggacaggtt
     8641 acagtagatg ggaagatggt ttcttttata gagcttggag tgggattcaa tccagaatta
     8701 acaggacgag aaaacgtcta tatgaacggc gccatgctgg gatttaccaa agacgaagtt
     8761 gatgatatgt acaatgatat tgttgatttt gcagagttgc atcattttat gaatcaaaaa
     8821 ttaaaaaatt attcttctgg catgcaggta cgtttagcat tttcagtggc tattaaagcc
     8881 caaggtgacg ttcttatttt agatgaggtg cttgctgtag gagatgaagc ttttcagcga
     8941 aaatgtaatg attattttat ggaaagaaaa gacagtggta agacgactat tttagtgaca
     9001 catgatatgg gagctgttaa gaagtattgt aaccgtgcag ttcttattga agatggtctt
     9061 gttaaagcat atggagaacc atttgatgtt gctaatcagt atagtgtaga taatacagag
     9121 acagctgagg atgcaatgaa tgctgaaaaa atatctgtat ctgatattgc caaagatttg
     9181 aaagtgtcat taatctcaaa tccaagaata acaccaaatg acacaatcac atttgaagtt
     9241 tcttatgaag ttttaaaaga tgattttacc tacattgcct tttctttaac ggatattgat
     9301 agaaacattt gggtttacaa cgacaattct ttagattatc caacacaagg tataggtaga
     9361 aaatgcattt cctaccaatg tcaactatct caacttaatg atataaaact aaaattagaa
     9421 gtaactgtcc gagataagaa cggtcagatg ctactctttt caacagcaga acaatcaccc
     9481 cagatagtga ttcaaagaaa cgatattgat caagatgatc tgtcagctct agattcagca
     9541 agtggcttgt atcaacgtaa tgggaagtgg gtttttcatt aatgagtaat agctagaagg
     9601 gaatttatgt ataaagtttc aattatttgc accaattata acaaagctcc ttggatatct
     9661 gatgcacttg acagtttttt aagccaagtg acagattttg aggttgaaat tattgttatt
     9721 gatgatgctt ctacagatga ctcacgagag attttaaaga gttatcaaaa aaagtcttca
     9781 ggaaaaatta aattactctt taacgaaact aatattggaa taacaaagac ttggataaaa
     9841 gcatgccttt atgcaaaagg caaatatatt gctagatgtg atggtgatga ttactggacg
     9901 gatagtttta aactccaaaa acaagttgat gtcttagaag cttccaaacg gtcacgatgg
     9961 tgtaatactg attttgactt tgtaaatagt caaggcgact tattatatgc taatgctttt
    10021 tcatctggtc atacaccttt gacagatact tacgaaaaag ttttagcctt aaaagggatg
    10081 acgatggcat cgacatggtt agttgatgct gatttaatga gacaagtaaa ccaaaaaatc
    10141 aatgtagata caccagacga tacttttgac attcaactag agttatttca actgactcaa
    10201 ctgacctata ttaaagactc aacgacggtt tatcgcatga caaccgattc tgattcaaga
    10261 cctactgata caccaaaaat gattcatcgt attcaaaaac tacttgatac tcagttaaat
    10321 tacttaaaaa agtataatca ggtagataca aaagaaatgt ctgaactatt attacaacaa
    10381 gatgctaagc aagaaatacg aattcatgag ttgagttgct ttattcaaga attacaacaa
    10441 actatcgtag ataaaacaaa gcagcaagaa acacgtgaag tggagttgca aaatgtcata
    10501 gaagaacaaa aaaatcaact atctgaatta agacaacaat accatgccat tattaattca
    10561 cgtcaatgga aatacacgtc taagctcatt gcttttatta ggagaaaaaa atgaatcgta
    10621 ttttattata tgttcacttc aacaaataca ataaaataag tgcacatgtt tactatcaat
    10681 tggaacaaat gagatcactg ttttctaaga ttgtttttat ctcaaatagt aaagtgagtc
    10741 acgaagattt aaaacgtctc aaaaaccact gtcttattga tgagtttttg caacgcaaaa
    10801 ataaaggttt tgatttttct gcatggcacg atggcttgat tactatggga tttgacaagt
    10861 tggaagagtt tgactcattg acgattatga acgatacttg ttttggtcct atttgggaga
    10921 tggctcctta ttttgaaaat tttgaagaaa aagagacagt tgacttttgg ggaattacta
    10981 ataatagagg tacaaaagct tttaaagaac atgtccaaag ttattttatg acgtttaaaa
    11041 atcaagtcat tcaaaacaaa gtttttcaac aattttggca atcgataata gaatatgaaa
    11101 acgttcaaga agtgattcag cattatgaaa cgcaattaac atccattctc ttaaatgaag
    11161 gctttagtta tcagacggtc tttgatacta gaaaagctga gtcgtcattc atgccacatc
    11221 cggatttttc atattataat ccaacagcaa ttttaaaaca ccatgtgcct tttataaaag
    11281 taaaagctat tgatgctaac cagcatatcg ctccttactt attgaatctg atacgagaaa
    11341 caaccaatta tcctatagat cttattgttt cacatatgtc gcaaatctct ttgccagatg
    11401 ctaagtacct tttatctcaa aaatacctaa actgccaacg gttagcaaaa caaacttgtc
    11461 aaaaggtagc tgttcattta catgtttttt acgttgattt gctagatgaa tttttaacag
    11521 ctttcgaaga ctggaacttc cattatgatc tttttattac aacagatagt gatatcaaaa
    11581 gaaaagagat caaagaaata cttcaaagaa aaggtaaaac tgctgatata cgcgttacgg
    11641 gaaatagagg aagagatatt tatccaatgt tattactaaa agataaactt tctcaatatg
    11701 attatattgg tcatttccat acgaaaaaat caaaagaagc tgatttttgg gcaggagaat
    11761 cttggcgaaa agaattgatt gatatgctcg tgaaaccagc agatagtatt cttagcgctt
    11821 ttgagacaaa tgatattggt attataatcg cagatattcc aagttttttt agatttaata
    11881 agattgtcaa tgcttggaat gagcatttaa tcgctcaaga aatgatgtct ttgtggagaa
    11941 aaatggatgt taaaaaacaa attgattttc aagctatgga tacatttgtt atgagttatg
    12001 gaacatttgt atggtttaaa tacgatgctc taaaatcact ttttgattta gaattaactc
    12061 aaaatgatat tccaagtgaa cctttaccac aaaactctat attacatgcg attgagcggt
    12121 tacttgtcta tatagcgtgg gggaattctt atgattttag gattgttaaa aatccctatg
    12181 agttaacacc ttttattgac aataaactgt taaatttacg tgaagatgaa ggagctcata
    12241 cctacgttaa ctttaatcaa atgggcggta tcaaaggagc attaaaatat attattgttg
    12301 gtcccgctaa agcaatgaag tatatattct tgcgtttaat ggagaaactt aaatgattaa
    12361 agacacattt ttaaaaacca attggttaaa tattagtcac catattaccc ttcttgtttt
    12421 cggtttttat ttcagttttt atagtttggc gaaagaacta gtaagctcca cggcacaacc
    12481 ggtaaactat tatactcatt tactaaatgt ttcttttgtg ggatatatta tatcactgat
    12541 tggattatct tattatttga gtcgccaagt tagtcgacag ttgtttttga aaactagttt
    12601 tattgtgata tcttatctaa ttgtcagcta ttgggtacaa ataacacagc acctgaatga
    12661 taaacggttt gatatctggt cattaactaa aaatcaattt tatcaatttc aagctctacc
    12721 ttctttactc attattttag tgatggccac tttaataaaa atattggtag catattttgc
    12781 aatagaaaaa gatagatttg ggctattagg ctatcaaggt aatacttttt ctgtagctct
    12841 gattttagca gttgtgccaa ttaatgacat acatctgtta aaactaataa gttctcgatt
    12901 ttctgaatta gtaagagcag gtaatagcca aattgcactg ttaaaaataa gtggactgtt
    12961 gatagtttta cttgtcatat ttgcaacaat catatacgtg gttttaaatg ctctaaaaca
    13021 ccttaagtca aataaacttt cattttcagt agcagctact actagtttgt ttttagcatt
    13081 agtttttaac tatacgtttc agtatggcgt aaaaggtgat gaagcattgc taggatatta
    13141 tgttttccct ggagctactc tttttcagat agtagctatt acactagttg ctattttagc
    13201 atacgtgata acgaatagat attggccaac taccttcttt ttgcttattc tgggaacaat
    13261 tatttctgtt gttaatgatt taaaagaatc aatgagaagc gagccgttat tagtaactga
    13321 ttttgtttgg ttacaagaat taggtttagt gacaagcttt gttaaaaaat cggtgattgt
    13381 agaaatggtt gtaggacttg ctatttgtat tgtggtagct tggtatctac atggccgagt
    13441 tttagcaggg aaattattta tgagccctgt caaacgggca agtgctgtat taggtttatt
    13501 tattgtatct tgtagtatgt taataccatt ttcttatgaa aaagaaggta aaatattatc
    13561 tggtcttccg attatttcgg ctttaaataa tgataatgat ataaactggt taggtttttc
    13621 aacaaatgct aggtacaaat ctttagcata tgtttggaca agacaggtga ccaagaaaat
    13681 aatggaaaaa ccgacaaatt atagccaaga aacaatagcg agtatcgctc agaagtacca
    13741 aaaattagca gaagatatta ataaagacag aaaaaataat attgctgacc aaacggttat
    13801 ttatctttta agtgaaagtt tgtcagatcc tgatagagta tcaaatgtta ctgttagcca
    13861 cgatgtttta cctaatatca aggcaatcaa aaatagcaca actgcgggac tcatgcagtc
    13921 agactcctac gggggtggaa ctgctaacat ggagtttcaa acgttaacaa gcttaccttt
    13981 ttataatttt tcttcttcag tatctgttct ttattcagaa gtctttccta aaatggccaa
    14041 acctcatacg attagtgagt tttaccaagg aaaaaatcgt attgcgatgc atcctgctag
    14101 tgctaacaat tttaatagaa aaacagttta tagtaattta ggtttttcca aattcttagc
    14161 tctatcgggt tctaaggata agtttaagaa cattgaaaat gtcggtttat tgactagcga
    14221 taaaactgtc tataataata ttttatcttt aattaatcct agtgaaagcc aatttttctc
    14281 agttattaca atgcaaaatc atattccttg gtcatccgat tatcctgaag aaattgttgc
    14341 tgaaggaaaa aatttcacgg aagaagaaaa tcacaaccta acaagttatg ctcggttatt
    14401 atcgtttact gataaggaaa caagagcatt tttagaaaaa ttaacacaaa ttaacaagcc
    14461 tatcacagtg gtgttttacg gagatcattt acccggttta tatcctgata gtgcttttaa
    14521 caagcatatt gaaaataaat accttactga ttattttatt tggagtaatg gtactaacga
    14581 gaaaaaaaat catccgctta tcaactcaag tgattttact gcagctttat ttgagcatac
    14641 tgattcaaaa gtatcacctt actatgcttt gttaacagag gtactgaata aagctagtgt
    14701 cgataaatca ccagatagtc ctgaagttaa agctattcag aatgatttaa aaaatgtcca
    14761 atacgatgtg actataggaa aaggttacct tttgaaacac aaaacttttt ttaagatatc
    14821 acgttaagaa aaatgctagg ctgtccgagt atagacgagc atcaaggttt ggtaatttgc
    14881 tcaaatgatt gtcaacagag tatctagggc caggtttaaa agattataat tcttaaggtc
    14941 ttattcgttt ataaaaaatg aatactcttt taaaatctta attgaaatga ataggagaag
    15001 ttttcgtgaa aaagttaatc attattcctg cttacaatga aagcagtaat attgtcaata
    15061 ctatacgtac tattgaatca gatgcctcgg attttgacta tatcattatt gatgattgct
    15121 caacggataa tacgttagca atatgtcaaa aacaggggtt caatgttatt tctttgccca
    15181 ttaacctggg aattggcggt gcggtgcaaa ctggctatcg ttatgcacaa agatgtggat
    15241 atgacgttgc agttcaagta gatggagatg gtcagcacaa tccatgttat ttggaaaaaa
    15301 tggttgaggt attagttcaa tcttcagtaa atatggtaat tggatcacga tttatcacaa
    15361 aagaagggtt tcagtcatca tttgctcgac gtatcggcat aaagtatttt acatggctta
    15421 ttgcgctact aacaggaaaa aaaataacag atgcaacgtc aggtctaagg ttaattgacc
    15481 gttcattgat tgaacgtttt gctaatcatt atccagatga ttatcctgaa cctgaaacag
    15541 ttgttgatgt attggttagt cattttaaag tgaaagaaat tcctgttgta atgaatgagc
    15601 gacaaggcgg tgtgtcatct atctcattga cgaaatcggt ctattatatg attaaagtga
    15661 ctttagctat tttagttgtt agattgaaag gaaatcgtta aaatgacggt aacagccagt
    15721 ttatttataa gttttattgt attaaccttt gttttctttt tgattaactt aatcaaaaaa
    15781 gacaaactag cgattaagta ctctttattg tggtttattt tagctttatt aattttacta
    15841 tttacgtggt tacctaacat attaaataaa atgtctcact tcctgggtat tcattcacca
    15901 accaatatgc tattcttttt aggtttttgt ttgagtttag ctattatttt ttcactgact
    15961 aataatatct cacttcaaaa tgataaagta aaacgtctga cccaagaagt ggcccttatg
    16021 aaaaaagaga aaaccaatga ttaatccttc aaaacaaaac caaacgattt ttttatggaa
    16081 tatgttagga agtttatcaa cagctgtaat atcagttata ttacttatgg tggttactag
    16141 gttactaact tcggctgatt cagatattta cgcctttgcc tattcttttg ctaatatgat
    16201 ggtagttgtt ggactttttc aggttcgtaa ttatcaagca acagatatta atgaaaaata
    16261 ttcctttagt cagtacttag tggcaagact gatgacttgt ttattaatgt tagccataac
    16321 agtaatttat ttaacgttaa ctaagacaga tagctacaag agtacaattg tctttctagt
    16381 ctgtttctat agatcgacag atgctttttc ggatttatat cagggaatgt tccaacaaca
    16441 tgaacggctg gatattgcag gtaaatcgct agcttacaga aatactctta tttttatggt
    16501 gtacacagca ataatattat attccaaaaa tcttacttta gcattagtag ctgtctgta
//
LOCUS       DAUDYX010000045        16357 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.045, whole genome shotgun sequence.
ACCESSION   DAUDYX010000045 DAUDYX010000000
VERSION     DAUDYX010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16357
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.045"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(<1..638)
                     /locus_tag="VHD02_001463"
     CDS             complement(<1..638)
                     /locus_tag="VHD02_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HEQ5498064.1"
                     /translation="MLTSKHHNLNKLVWRYGLTSAAAVLLAFGGGASSVKAEGSAAPL
                     TSEQRQQEIEKIKQEIQAKYPKLTEEKYHSYGVFGRKYLDYDKYQKGLEEYTQEALKK
                     IVELIEKGATQGPAGPKGETGPAGPAGPEGKPGKAGEKGDRGEKGEAGIQGPQGEKGD
                     TGAQGPQGEAGAPGEKGEKGDRGETGAQGPVGPQGEKGETGAQGPQGEAGKPG"
     gene            1019..1480
                     /gene="nrdI"
                     /locus_tag="VHD02_001464"
     CDS             1019..1480
                     /gene="nrdI"
                     /locus_tag="VHD02_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEQ5498065.1"
                     /translation="MPQITLVFISLSGNTLSFVKRLSLYLADNYDYHVKQINIKDLKH
                     ETFPVKEEFVAILPTYLEGGNGVDSGEVEILTTPLGEFIAAHGNAQHCLGIIGSGNKN
                     FNHQYCLTAKQYAKRFGFPLLGDFELRGTPDDISRLAQVIMEASSRHSSNS"
     gene            complement(1875..2693)
                     /locus_tag="VHD02_001465"
     CDS             complement(1875..2693)
                     /locus_tag="VHD02_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEQ5498066.1"
                     /translation="MTKVLTLNTHSWMQANTLKKLVALAEHILAEKYDIICLQEINQL
                     IESELATDLPRYQALSGTPSIHKDHFALLLIHYLQKRGQHYYWSWAYNHIGYDIYQEG
                     VAILSKQPIHVSDILVSAMADETDYHTRRSLIAKTTLDGKEVAVVNVHLSWFDKGFLG
                     EWEKLEKELLTLNCPLLLMGDFNNPTDQDGYQVMMGSPLNLQDSHKVADHVFGDHSIV
                     ADIDGWQGNKEALKVDHVFTSKDFIIRSSKITFEGGDAPVVSDHYGLEVTLDWR"
     gene            complement(2776..4962)
                     /locus_tag="VHD02_001466"
     CDS             complement(2776..4962)
                     /locus_tag="VHD02_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEQ5498067.1"
                     /translation="MKTSFKQLFRFEFWQKFGKCLMVVIAVMPAAGLMISIGNSIPMI
                     NHDSAFLASLGNIIAQIGWAVIVNLHLLFALAIGGSWAKERAGGAFASGLAFVLINRI
                     TGAFYGVSSAMLADPEAKITSLLGTQMIVKDYFTSVLESPALNTGVFVGIIAGFVGAT
                     AYNKYYNYRKLPEILTFFNGKRFVPFVVILRSIFVALILVVVWPVIQSGINSFGMWIA
                     SSQDSAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYEVMTGAAAGTKVFGQ
                     DPLWLAWVTDLVHLKGSDASAYSHLMDSVTPARFKVGQMIGATGTLMGVALAMYRNVD
                     ADKKHTYKMMFISAAAAVFLTGVTEPLEYLFMFAAMPLYIVYALVQGASFAMADLVNL
                     RVHSFGNIELLTRTPMALKAGLGMDVINFVWVSILFAVIMYFIADMMIKKMHLATAGR
                     LGNYDADILGDRNTQATPTQVADSNSQVVQIINLLGGSGNIDDVDACMTRLRVTVKDP
                     AKVGAEDDWKKAGAMGLIQKGNGVQAVYGPKADILKSDIQDLLDSGALIPEVNMSQLT
                     SKPTPAKDFKHVTEEVLSVADGMVLPITGVKDQVFAAKMMGDGFAVEPTHGNIYAPVA
                     GLVTSVFPTKHAFGLLTDNGLEVLVHVGLDTVALNGVPFSVKVSEGQRVHAGDLLVVA
                     DLAAIQSAERETTIVVAFTNTTEIQDVTLTSLGNQPAKTKVATVEL"
     gene            complement(5319..6068)
                     /locus_tag="VHD02_001467"
     CDS             complement(5319..6068)
                     /locus_tag="VHD02_001467"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEQ5498068.1"
                     /translation="MQQYFIKGKAEKKVTITDKDTIKHMFQVMRLTDEAEVVLVFDDG
                     VKYLAKVTNSMAHELEIIEILPDQVELPVKVTIASGFPKGDKLDTIAQKVTELGASAL
                     WGYPADWSVVKWDGKKLAKKEDKLAKIVLGAAEQSKRNRVPEVHLFEHKAEFLKSLSS
                     FDHIFIAYEETAKAGQLATLAREVKEVKPGAKILFIFGPEGGISPAEITQFEAAGAIK
                     VGLGPRIMRTETAPLYALSALSYALELQASK"
     gene            complement(6068..7021)
                     /locus_tag="VHD02_001468"
     CDS             complement(6068..7021)
                     /locus_tag="VHD02_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEQ5498069.1"
                     /translation="METWQEVTVHVHRDAQEAVSHVLIETGSQGVAIADSADYIGQKD
                     RFGELYPDVEQSDMIAITAYYPSSTNLADVIATINEQLAELASFGLQVGQVTVDSQEL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYDASAGEKVIKLDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVIDVGTGSGVLSIASSLLGAKTIYAYDLDDVAVRVAQENIDLNQ
                     GTDNIHVAAGDLLKGVSQEADVIVANILADILVLLTDDAYRLVKKEGYLILSGIISEK
                     LDMVLETAFSAGFFLETHMVQGEWNALVFKKTDDISGVIGG"
     gene            complement(7092..7562)
                     /locus_tag="VHD02_001469"
     CDS             complement(7092..7562)
                     /locus_tag="VHD02_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEQ5498070.1"
                     /translation="MAKYGFLSVLEEEMDKHFQYDYAMDWDKKNHAVEVTFVLEAQNK
                     EAIKTIDDSGEVTQDDIVFEDYVLFYNPAKSQFDAADYLVTIPFDAKKGFSREFLAYF
                     AQFLNDVAIEGHSDLMDFLADDSKADFFLEWNAQAFEEGQQGLEEAASYPYPRY"
     gene            complement(7627..7848)
                     /locus_tag="VHD02_001470"
     CDS             complement(7627..7848)
                     /locus_tag="VHD02_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEQ5498071.1"
                     /translation="MFDYIEGLIERRHSFIKEHFVASIDNFLNFREYRMLEGKGSISM
                     KFAKEKASREYVEFNKLQKINPDKGHKKI"
     gene            8038..8154
                     /locus_tag="VHD02_001471"
                     /pseudo
     CDS             8038..8154
                     /locus_tag="VHD02_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            8256..9119
                     /locus_tag="VHD02_001472"
     CDS             8256..9119
                     /locus_tag="VHD02_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISSpy2 family transposase"
                     /protein_id="HEQ5498072.1"
                     /translation="MSHLQYTAKSHHLQWNVTQLSTICHQLYRDYCPEGLKHRRNISL
                     SKVSDESLLVLLVLQAELGIKSQRHFYRICQLFPCGHLLERSRFNRRARQLIWLVQVI
                     RQAMNARISPDTIVIIDSFPLPLCQPVRNHRAHIFKGVADIGYNTSKHLWFYGFKVHM
                     LVTLSGYILNYVVTPASVHDIKAVHELLEECDQSIILADLGYLSRELKNNLEQKGYQL
                     WTPLRQNMTGAKQHNHWRVMAMRRTIETRFSELCSLFDIEHTLTRGVTGLQLRIEQII
                     LAYNLKYVEFN"
     gene            complement(9263..9604)
                     /locus_tag="VHD02_001473"
     CDS             complement(9263..9604)
                     /locus_tag="VHD02_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498073.1"
                     /translation="MKKTLLLSAAALMVASTVALSTNSVSASSYLNNPYYNTSRFLRQ
                     NGTQVLREARYASELKIRNLLDQYGIVGEEYKGYFNCYYRQARQALSLGQVDDVINSL
                     ERNLQERQDND"
     gene            10338..12095
                     /gene="pabB"
                     /locus_tag="VHD02_001474"
     CDS             10338..12095
                     /gene="pabB"
                     /locus_tag="VHD02_001474"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEQ5498074.1"
                     /translation="MHRKTIIDFKELGQRYLFEEPLVELVAKSLDQVGPVIEKVQHYQ
                     QLGYYVVGYLSYEAAAFFDNALQTHNDRLGNEYLAYFTVHKTCQKKDLPLDYDSITIP
                     NQWVSATQKEAYQKAIETIHREMQQGNTYQVNYTIQLTQELNAADSLAIYNKLVVEQA
                     AGYNAYIAHDEFAVISASPELFFKQEGNRLTTRPMKGTTKRGINSWLDQQEHDWLQAD
                     GKNRSENMMIVDLLRNDMGKICQIGSVCVDRLCEVERYSTVWQMTSTIVGDLKADCDL
                     IDILTALFPCGSITGAPKVSTMAIITSLEPKPRGIYCGSIGICLPDGRRFFNVPIRTI
                     QLSHNQATYGVGGGITWQSKWEDEYEEVHQKTAFLYRHKQIFDLKTTAKVEHKKIALL
                     EQHLNRLKEAATYFAYPYNEKALQKQLSTYLENKDNAAYRLTISLSKDGKMRLSDQPL
                     EPLSADFLTAQLSLQDKDVTASPFTYFKTSYRPHIEQKSYEQVFYNQAGQLLETSIGN
                     LFIQLGQTLYTPPVAVGILPGLFRQELLATGQAQEKEVTLADLKEASAIFGGNAVRGL
                     YPLNLELTHLDALLAKSQA"
     gene            12128..12694
                     /locus_tag="VHD02_001475"
     CDS             12128..12694
                     /locus_tag="VHD02_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEQ5498075.1"
                     /translation="MILLIDNYDSFTYNLSQYLSEFDETIVLYNQDPNLYDMAKKANA
                     LIFSPGPGWPKEANQMPKLIQDFYQTKPILGVCLGHQAIAETLGGTLRLAKRVMHGRQ
                     STIETQGPASLFRSLPQEITVMRYHSIVVDQLPKGFSVTARDCDDQEIMAFEHHTLPL
                     FGLQFHPESIGTPDGMTMIANFIAAIPR"
     gene            12727..13995
                     /locus_tag="VHD02_001476"
     CDS             12727..13995
                     /locus_tag="VHD02_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEQ5498076.1"
                     /translation="MPDHLALRMRPKTISEVIGQKHLVGEGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTRYAFRTFNATIDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGTIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKTAIQ
                     LAISDKERGFPFLVTIDDEALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDGSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPDAQVHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYL
                     AMDAALADLRTSGNLPIPRHLRDGHYAGSKELGNAKDYLYPHAYPEKWVKQQYLPDKL
                     VGHHYFEANETGKYERALGSNKERIDKLSD"
     gene            14084..14280
                     /gene="ssrS"
                     /locus_tag="VHD02_001477"
     ncRNA           14084..14280
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VHD02_001477"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            14281..14353
                     /locus_tag="VHD02_001478"
     tRNA            14281..14353
                     /locus_tag="VHD02_001478"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14314..14316,aa:Lys,seq:ctt)
     gene            14494..14934
                     /locus_tag="VHD02_001479"
     CDS             14494..14934
                     /locus_tag="VHD02_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ5498077.1"
                     /translation="MKIIEYKGNYRDDLIFMVLEAKNALRRIPSINPDLLTIETSYLE
                     KGDCFWLAVNEESRVIGSIAYSSIPKTNEVFLHRLFIKPSLKHQGIGSQLLTFAEDYL
                     RKIGKSHVRIHLGQPKEQWFESYRFYPKHGYQFYDDTHLIKKLI"
     gene            14989..15459
                     /locus_tag="VHD02_001480"
     CDS             14989..15459
                     /locus_tag="VHD02_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEQ5498078.1"
                     /translation="MKIAIIGHSGSGKSTLARFLGQHYHCEVFHLDQLHFSSNWQERS
                     DHDMIADLSTCLLKQDWIIEGNYANCLYEERMSEADYIIYVNFSRFHCVYRAFKRYLN
                     YRGKTRPDMADNCQAKFDVAFVKWILLDGRSRNQLKKYQSVVQKYSHKTIVLTN"
     gene            15744..>16357
                     /locus_tag="VHD02_001481"
     CDS             15744..>16357
                     /locus_tag="VHD02_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEQ5498079.1"
                     /translation="MKKTKLIFSFTSIFIAIISRPVFGLEVDNNSLLRNIYSTIVYEY
                     SDTVIDFKTSHNLVTKKLDVRDARDFFINSEMDEYAANDFKAGDKIAVFSVPFDWNYL
                     SKGRVTAYTYGGITPYQKTSIPKNIPVNLWINGKQISVPYNEISTNKTTVTAQEIDLK
                     VRKFLISQHQLYSSGSSYKSGKLVFHTNDNSDKYSLDLFYVGYR"
BASE COUNT         4831 a         3573 c         3117 g         4836 t
ORIGIN      
        1 cctggtttgc ctgcttcacc ttgtgggcct tgtgcgcccg tttcaccttt ttcaccttgt
       61 gggcctactg gaccttgagc tccagtttca ccgcggtcac ctttttcacc cttttcgcct
      121 ggagctccag cttcaccttg tgggccttga gctccggtgt cacctttttc accttgtggg
      181 ccttggattc cagcttcacc tttttcaccg cggtcgcctt tttctccagc tttacctggc
      241 ttaccttctg gaccggctgg gccagcagga ccagtctcac ctttaggacc ggctgggcct
      301 tgtgttgcac ctttttcaat taattctaca atttttttaa gtgcttcttg agtatactcc
      361 tctaatcctt tttgatattt atcgtaatca agatattttc tgccaaaaac tccataactg
      421 tggtattttt cttcagtaag ttttggatat ttagcctgga tttcctgttt aattttttca
      481 atttcttgtt gtctctgctc cgatgtcaga ggagcagctg aaccctcagc cttaacgctg
      541 cttgccccgc ctccaaaggc tagaaggacg gcagcggctg aggttagccc gtagcgccag
      601 accagtttgt tgagattatg gtgttttgat gtcaacatat gttgttctct ctttctctat
      661 tagatgatgt gttttagata aaatatcatg gtagctagac ctgattattt ataatagtat
      721 tatatagagt gtgaaaacga ttgtaaagtt ctcttttgtt gttcatcttt tagattttga
      781 ctgattgctc ttttgtggag atcagagggc cactttggca cttgcctgcc tcgtttttaa
      841 gtcgagagca tccggatcct ttctttaagg tcactctttt ttgaaatgtt actttttttg
      901 actgcttgta ccttcttgcc tggttgaata ccgctaggtt tcttctttac ttgtttgttg
      961 gtcttgattg tggtaagctt atgaagaaac cttgtaggat aaaaaaaagg agacacttat
     1021 gcctcagatt acccttgttt ttattagcct cagtggcaac acccttagct ttgtcaagcg
     1081 attatccctt tatttagcag acaattacga ttaccatgtc aaacagatta acattaaaga
     1141 tttgaagcac gagacttttc ccgtcaaaga agagtttgtg gcgattctac ccacttatct
     1201 agaaggcgga aatggcgttg actctggtga agtggagatc ctcaccactc ctttgggaga
     1261 gtttattgct gcccatggca acgcccagca ttgtcttggg attattggtt caggcaacaa
     1321 aaactttaac catcagtatt gcctaacagc caaacagtat gccaaacgct ttggctttcc
     1381 actgctaggt gattttgagc tacgagggac tcctgatgac atttcccggt tagcccaggt
     1441 catcatggag gccagctcca gacactcgtc taacagctag acagtctata gatgatcata
     1501 gctgttttaa aaaagagcgc ttttaggaca ccaattaggg aagtccccta ccctacgcct
     1561 tgtgatttct tagcctttga aaagagcgat cacagtgctt cttggggctt gggatcacac
     1621 caagcactcc tagaaatacc cccatgcaga ctttagcaag gcaactgaac gcttggtggt
     1681 actttctgtg aagctcagat ggttggtgac aaaaaaagcc ctgtttttct agggttactg
     1741 acatgactaa agaggtctaa ccagctctat cttgagctgg ttagacctct ttgtgttctc
     1801 ctaatccccc tagacgatcg cagtttctta tgtgtcttgg cttctcctat gtggtgtgcc
     1861 tgttagcaca tgctctaacg ccagtctaat gtcacttcta gaccatagtg gtcactgacg
     1921 actggcgcat ccccgccttc aaaggtaatc ttagaagacc ggatgatgaa gtccttactg
     1981 gtaaagacat ggtccacctt gagggcttct ttgttacctt gccagccatc aatatcagcc
     2041 acgatgctat gatcaccaaa cacatggtca gcaaccttat ggctatcttg taggtttagg
     2101 gggctaccca tcatcacttg ataaccgtct tgatccgttg gattgttaaa atctcccata
     2161 agtagcaagg gacagtttag tgtgagcagc tctttttcta atttctccca ctcccctaaa
     2221 aaccccttgt caaaccatga caagtgcaca ttgacaacag cgacttcttt gccatctaac
     2281 gtggttttgg ctatgagtga gcgtctggtg tggtagtctg tttcatcagc catcgctgac
     2341 acgagaatat cggaaacatg aataggttgt tttgataaga ttgccacgcc ttcctggtaa
     2401 atgtcatagc caatatgatt ataagcccaa gaccaataat agtgctgccc tcgtttttgt
     2461 aaataatgga tcaagagcag ggcaaaatga tctttatgaa tgcttggtgt ccctgaaaga
     2521 gcttgatatc ttggtaaatc tgtggccagc tcgctctcga ttaactgatt gatttcttgc
     2581 aagcaaatga tatcgtattt ttcagctaaa atatgctcag ccaaagcaac tagtttttta
     2641 agagtgttgg cttgcatcca cgaatgggta ttcagtgtta ataccttggt cattaatcct
     2701 cctttataag acaagaagag ccatagggcc taaaccagct atggctatta cctatcacta
     2761 tctcgtgatg ttttgttaaa gttctacggt ggctacttta gtcttagcag gttgattgcc
     2821 taaagaagtc aaggtcacat cttggatctc tgttgtgttg gtaaaagcaa caacaatggt
     2881 tgtttcacgc tctgctgatt ggatggcagc aagatctgct acgactaata ggtcacctgc
     2941 atgaacccgt tgcccttctg agactttgac tgaaaaaggc acaccattta aggcgacggt
     3001 atcaagtccc acgtgcacca gcacttcaag accattatct gtcagtaaac caaaggcatg
     3061 cttggtcgga aagacactgg tcacaaggcc agctacgggt gcatagatat taccatgggt
     3121 tggttcaacc gcaaacccat ctcccatcat cttagcagca aacacctggt ctttcacacc
     3181 tgtgattggg agaaccatcc cgtctgccac tgatagcact tcttctgtca cgtgtttaaa
     3241 atcttttgcg ggagtcggtt tgctagtgag ctgtgacata ttgacttctg gaataagagc
     3301 acctgagtcc agcaaatctt gaatatctga tttcaaaata tctgctttag ggccatagac
     3361 cgcttgaacg ccgttgcctt tttggatcaa gcccatagcg ccagcttttt tccaatcgtc
     3421 ctcagcgcca accttagcgg ggtctttgac cgtcactcgt aagcgcgtca tacaagcatc
     3481 aacatcatca atattgcctg acccaccaag gagattaatg atttgtacga cttgagagtt
     3541 gctatctgct acttgggtcg gtgttgcttg agtgttacgg tcacccagta tatcggcatc
     3601 gtagttacct aaacggccag ctgttgcgag atgcattttc ttgatcatca tatccgcaat
     3661 aaagtacatg ataactgcaa agaggataga aacccaaaca aagttaatca catccatgcc
     3721 taggcctgct ttaagggcca taggggtacg ggtcaataat tcaatgttcc caaatgagtg
     3781 aacacgaagg ttcacaaggt cagccatggc aaatgaagct ccttgaacaa gggcatagac
     3841 gatgtaaagt ggcattgctg caaacataaa taggtactca agtggctcag ttacccctgt
     3901 caaaaagacc gctgccgctg ctgagatgaa catcattttg tatgtgtgtt ttttatcggc
     3961 atctacatta cggtacatgg ctaaggcaac ccccatcaat gttccggtag ctccaatcat
     4021 ttgtcctacc ttaaagcgag ctggagtcac actatccatt aagtgactat aggctgaagc
     4081 gtctgatcct ttaaggtgaa caaggtcagt tacccaagca agccacaaag ggtcttgacc
     4141 aaatactttt gtccccgctg ctgcacctgt catgacctca taagttccac caagagctgt
     4201 atagttcatt gggatcgtca acatgtggtg aagaccaaat ggcaacaaga gacgttccaa
     4261 ggtaccatat aggaatgggg ctaggattgg agctgaatct tgtgaagagg caatccacat
     4321 cccaaaactg ttaatcccag actggatcac cggccaaaca acaaccaaaa taagggctac
     4381 aaaaatagaa cgtaaaataa caacaaatgg tacaaaacgc ttcccattaa agaaggtcaa
     4441 aatttcagga agtttacgat aattatagta tttgttatag gccgttgctc ctacgaaccc
     4501 tgcaataatc ccaacaaaaa cccctgtgtt taaagctgga gattccaaga cactggtgaa
     4561 ataatcttta acgatcattt gagtgccaag gaggcttgtg atttttgctt ctggatcagc
     4621 tagcatagca ctagacacgc cataaaaagc acctgttatc cgattgatca agacaaaagc
     4681 aagacctgag gcaaaggccc ctccagctct ttctttagcc caactaccac caatggccaa
     4741 ggcaaatagc aagtgaaggt taacaataac agcccaacca atttgagcga taatgttgcc
     4801 tagagatgcc aaaaatgctg agtcgtggtt aatcattgga atagagtttc cgatactgat
     4861 cattaaacca gccgctggca taacagcaat aacgaccata agacatttcc caaatttttg
     4921 ccaaaattcg aaacgaaaca gttgtttgaa tgatgttttc attctatctc tcctttatta
     4981 tcccctttta gccatagctg cccttaaggc gcttttgtga cacaatgata tagactacaa
     5041 ctcagcgatg caatcgcttg cattgatctg atacctttat tatactagcc tcgcagcaat
     5101 ttgtaaagcg ttttcttaga cagatgttag tcgtttttct tcattaaatg gtagtgtaac
     5161 ttaccaaaaa gaggacaaga gcccatttcc ttttttggtg atgagcccat caacgctctt
     5221 caaaaaaaca agaagcaata aaaaaaggcc gttgacacaa tcgtgaactg cctgttcgtt
     5281 tctttagcct cttctttcta aagtaagcag cagcttactt attttgatgc ctgcaattcc
     5341 aaagcataac taagagcact taaggcatat agcggcgctg tctctgttcg cataatgcga
     5401 gggcctaagc ccaccttgat ggcacctgct gcctcaaact gagtgatttc agctggggaa
     5461 attccccctt ctggtccaaa gataaagagg atttttgccc ctggcttaac ttcttttact
     5521 tcacgcgcca aggtcgctag ctgacctgcc ttagctgttt cctcataagc aatgaagata
     5581 tgatcaaagc tagaaaggct ttttagaaac tctgctttat gttcaaaaag gtgaacttca
     5641 ggtaccctat ttcgtttgct ctgctcagcc gctcctaata caatcttagc caatttgtct
     5701 tcttttttag ctagtttttt gccatcccat ttgactactg accagtctgc ggggtaaccc
     5761 catagcgcac tggctcccaa ttcagtcact ttctgggcaa tcgtgtctaa cttatcgcct
     5821 ttaggaaatc cagaagcaat agtcacttta acaggcaatt ccacctgatc tggcaagatc
     5881 tctataatct ctaactcatg agccatacta ttggtcacct tggctaaata cttcacccca
     5941 tcatcaaaga ccaagacaac ttcagcctcg tcagtcaagc gcatcacttg aaacatgtgc
     6001 ttgatggtat ctttatcggt aatcgttacc tttttttctg ctttgccttt aataaaatac
     6061 tgttgcatta gccgccaatc actcctgaga tatcatctgt ttttttaaag acaagggcat
     6121 tccactcacc ttgaaccata tgcgtctcaa ggaagaatcc tgcgctaaaa gcagtttcca
     6181 gaaccatatc caatttctca gaaatgatgc ctgataagat aaggtaacct tcctttttga
     6241 ccaaacggta agcatcatca gtcaataaga ctaagatatc cgccaagata ttggctacga
     6301 tcacatccgc ttcttggcta acccctttta aaaggtcgcc agcagccaca tgaatgttgt
     6361 ctgttccttg attcaaatca atgttttctt gagccactcg aacagccacg tcatctaagt
     6421 cataagcgta gatggtttta gcacctagta aggaactggc aatcgagagg acacctgatc
     6481 cggtccctac atcgataact gtctcaccac cacgaaggac ttgttccaag gcaaaaaggc
     6541 tcatcttagt tgtgggatgt gtccctgtcc caaatgccat tccaggatct aatttaatca
     6601 ctttttcccc agcagaagcg tcataatctg tccaactagg gacaatggtt aaatcgtgcg
     6661 tgatacgagc tggttcatag tattttttcc aattgtctgc ccagtcttcc tcagccaatt
     6721 cttgactatc cacagtaacc tgaccaacct gtaacccaaa actagctaac tccgctaact
     6781 gttcattaat ggtcgcaata acatccgcca aattagtgct acttgggtaa taggctgtga
     6841 tagctatcat gtcagactgc tccacatctg ggtacaactc cccaaagcgg tccttttgcc
     6901 caatatagtc tgcgctatcc gcgatagcaa ccccttgact gcctgtctca atcaacacat
     6961 gtgaaacagc ctcttgcgca tcacgatgca catgaaccgt cacttcttgc catgtttcca
     7021 tagttatgtc ctttctattt tgaccttttg gttattgtcc taatagtgtt agcaccatct
     7081 cgtgtgtcac cttagtacct tgggtaaggg taacttgctg cttcttctag tccttgttga
     7141 ccttcttcaa aagcttgcgc gttccactcc aagaagaaat cagccttgct gtcatctgct
     7201 aagaaatcca tcaaatcgct gtgcccttca atagcaacat cgttgagaaa ttgcgcaaag
     7261 taagctaaga attcacgaga aaagcccttt ttagcgtcaa aaggaatggt aaccaaataa
     7321 tctgctgcat caaattgaga cttggcaggg ttgtaaaaga gcacatagtc ctcaaaaaca
     7381 atatcatctt gggtcacttc gccactatca tcaatggttt ttatggcctc tttattttga
     7441 gcttctaaca caaaagtcac ttcaaccgca tgatttttct tgtcccaatc catagcataa
     7501 tcatattgaa aatgcttgtc catttcttct tctaatactg ataaaaagcc gtattttgcc
     7561 atctttttta tacctaatct ttctaactgt tgctgtataa ggctttgtaa gacctcatta
     7621 gtttacttaa atttttttgt gtcccttatc agggttaatt ttttgtaact tattaaattc
     7681 tacatactca cgactagctt tttcttttgc gaatttcata gaaatagagc ctttaccttc
     7741 tagcattcta tattcacgga aattaagaaa gttatctata cttgcaacaa aatgctcctt
     7801 aataaatgaa tgacgtctct ctatcaaacc ttcaatgtaa tcaaagatgg attattgcct
     7861 ttgacaatat gatcaacact aatattatag cacaaaggag ctaacgatgg gtgctgaaga
     7921 acaactgaaa gaattccata cgccaatagc cttgatttga tgtataacga gctttaccag
     7981 tttgatatag aatatcattc ctttagacgt aaactccttg aaataagccg agaatgcgcc
     8041 ctagaggaat tagacgccat caagacaaga gactacgttc attggtggaa ctatcatcgc
     8101 attcacagta gcctcagtta ccaaacacat atgaccttga gaaacatcgc ctaactaaat
     8161 atactttact cgtggtgcta gttaatgtca gaatacaata aaaagcccaa aaggactata
     8221 ctgtaagtgt tcaaacaaac aggaggttag ccccaatgag ccacttacag tataccgcta
     8281 aatcccatca cttacaatgg aatgtcacac aattatctac gatttgccat cagctatatc
     8341 gtgattattg tccagaaggc ttaaaacacc gtcgtaacat tagcttgtcc aaggtctcag
     8401 atgagtctct actagtcttg cttgtcttgc aagctgaatt aggtattaag tcacaacggc
     8461 atttttatcg tatctgtcaa ctctttcctt gcggtcatct ccttgaaaga agtcgtttta
     8521 atcgacgagc aagacaatta atttggttag tccaagtcat tagacaagcc atgaatgctc
     8581 gaatttctcc tgatacaata gtcatcatcg acagttttcc tctaccactt tgtcaacctg
     8641 tccgtaatca tagagcccat atttttaaag gagtggctga tattggttat aacacctcta
     8701 agcacctgtg gttctatggg ttcaaagtcc acatgttagt gactttatca ggctatattc
     8761 ttaactatgt ggtcacacca gcttctgttc atgatattaa agcggttcac gagttattag
     8821 aagaatgtga ccagtccatc attttagcag acttgggcta ccttagccgt gagctgaaaa
     8881 ataatctgga acaaaaaggc tatcagctat ggacaccctt gcgtcaaaat atgacagggg
     8941 ctaaacagca taatcattgg agagtaatgg ctatgagacg aactattgaa actcgatttt
     9001 cagaactttg ttcccttttt gatatagaac acacactgac tagaggagtt acaggattac
     9061 aattgagaat cgaacaaatt atccttgctt ataatctgaa atacgttgag tttaactagc
     9121 accacgagta tactttataa atgttgcaca gaaaactgtt gccttttcaa agcttggctg
     9181 gagtttgtgg aacattacag gggtggagga ctaaagcaac aaaaaaagaa gcaagagatg
     9241 aattgcttct tttacagata gattagtcgt tgtcttggcg ttcttgtaag ttcctttcca
     9301 aactattgat gacatcatca acttgtccta aacttagagc ttgacgagct tgcctatagt
     9361 agcagttaaa atagccttta tattcctcac caacgattcc atactgatct agtaggtttc
     9421 taatttttag ctcggaagca tatctagctt ctctaagaac ttgagtgccg ttttgtctta
     9481 gaaagcggct agtattatag taaggattat ttaagtatga agatgcactt acagaattag
     9541 ttgatagagc aacggtgcta gctaccatta atgcagcggc acttagtaat agtgtttttt
     9601 tcataaaaaa taacatttag gaatagtttt ctttaaatta ttttattaga acactgaaaa
     9661 aacaagatag aaatatcact gatatagtct ctgtaacaaa aaataatctt cgtattttaa
     9721 gctatattac gatgcataat cctctcatta gtcaatgaaa acggataaat aaaacacaat
     9781 taaatttaac ataattaaca aaggcggtgg ttgttgatcg aaatgaagat gccatagctg
     9841 agatacataa tgtaataaat caagtaaata agactgcgcc aaaaataaaa gatagtgagc
     9901 actttgataa tttattaaag aactctcata ataatctcgt aataaggttt ccgttgaatt
     9961 aaacaacata atatcaataa ccaaagatcc attctattat gatgaaattt attctggagc
    10021 tgtaaaagaa ctattggcta aatgtttgtc ttatgttgat tatttgaaag atttagctaa
    10081 gcaatctaag acagaaccta attttggtga aaatcataaa ttatataaaa ctattatgga
    10141 tgagttcaag agtcaagtta aaacactggc attgaaagat aaaaaaatgg aatactgctc
    10201 acctcacgat aagagtatat tgtagtcaat caagactgaa cattcttagt gaacgttcag
    10261 tctttttacg tggccaaaag acagttgtta cctgttctca ttaggcttag caaccgcttt
    10321 tatgatataa tttgggtatg catagaaaaa cgattattga tttcaaagag ctcgggcaac
    10381 gctatctttt tgaagagcca ttagtagaat tggtagccaa gtccttagat caggtcggtc
    10441 cagtcattga aaaagtacaa cactaccaac agctgggcta ttacgtggtg ggctatctca
    10501 gttatgaagc agctgctttt tttgacaatg ccttacaaac ccataatgac agactaggca
    10561 acgagtacct tgcctatttt acagtgcaca aaacctgtca aaaaaaagac ctacctcttg
    10621 attatgacag cattactatc cctaaccaat gggtcagcgc aactcaaaaa gaagcctatc
    10681 aaaaagccat tgaaaccatt caccgtgaga tgcaacaggg caatacttat caggttaact
    10741 ataccattca attaactcaa gaactcaatg cagctgacag cttagccatt tacaataaac
    10801 tcgtcgttga acaggctgct ggctacaatg cctatatcgc ccatgatgag tttgccgtta
    10861 tttcagctag tcctgagttg ttctttaaac aagagggcaa caggttaact actagaccta
    10921 tgaaaggcac caccaaacga gggattaata gctggctgga tcaacaagaa catgactggc
    10981 tccaagctga cgggaaaaat cgctctgaaa acatgatgat tgtggatttg ctccgcaatg
    11041 acatgggaaa aatttgccaa ataggcagtg tttgtgttga taggctctgt gaggtcgagc
    11101 gatactctac cgtctggcaa atgacctcaa ctattgttgg tgacctcaaa gcggactgtg
    11161 acctgataga cattctcaca gctctctttc cttgtggctc tatcacgggg gctcctaaag
    11221 tctccaccat ggcaattatc acatctctgg agccaaaacc aagggggatt tattgtggaa
    11281 gtattggcat ttgcctacct gatggtcgcc gtttcttcaa tgttcccatt agaaccatcc
    11341 agctcagtca taatcaagct acttatggtg tcggaggcgg catcacttgg caaagcaagt
    11401 gggaagacga atacgaagaa gtccatcaaa aaacagcttt tctctatcgg cacaaacaaa
    11461 tctttgacct caaaacaacc gccaaggtgg aacacaaaaa aatagctctc cttgagcaac
    11521 acctcaatcg ccttaaagaa gcagccactt attttgctta cccttataat gaaaaagctt
    11581 tgcagaaaca actgtcaacc tatttggaaa ataaggataa tgctgcctac cgcttgacga
    11641 ttagtttatc caaagatgga aagatgagac tctctgatca gcctttggag cctttatcag
    11701 ccgattttct aacagctcag ctctctttac aggacaagga cgtgacagct tcgcctttta
    11761 cctactttaa gaccagctac cggcctcata ttgagcaaaa atcttatgaa caggtgttct
    11821 ataaccaagc ggggcaatta ttggaaacgt ccatcggcaa tctttttatc cagctgggtc
    11881 agaccctcta cacaccacca gtggctgtgg ggattttacc aggacttttt cgccaagagc
    11941 tgctagctac tggtcaagct caggaaaaag aggtgacact agctgattta aaagaggcta
    12001 gcgctatttt tggaggaaat gctgtccgtg gcctctatcc tctcaacctt gagcttactc
    12061 accttgatgc cctcttagct aagagtcaag cctaaactgc cactcatctt ttgttattaa
    12121 ggaacctatg atactcttaa ttgataatta cgattcattt acctacaacc tctcacaata
    12181 tctaagtgaa tttgacgaga cgattgtctt gtataaccaa gacccaaact tatatgacat
    12241 ggccaaaaaa gctaacgctc taatcttttc acctggtcct ggttggccca aggaagccaa
    12301 ccaaatgcca aaactcattc aagactttta ccaaacaaaa cctatcttag gagtgtgtct
    12361 gggacaccaa gctatcgctg aaactctagg aggaacctta cgcttggcaa aacgcgtcat
    12421 gcatgggaga caaagcacca ttgaaacgca aggccctgct agtctttttc gctccctgcc
    12481 acaagagatc accgtcatgc gttaccattc tatcgttgtg gatcagttac caaaaggttt
    12541 tagcgtaact gctagagact gtgacgatca agaaatcatg gcctttgaac accacaccct
    12601 gccacttttt gggctacaat ttcacccaga aagcatcgga actcctgatg ggatgaccat
    12661 gatcgccaac ttcatcgcag ccattccccg ttaatctcat cccccaaaag aaaggaggac
    12721 agttacatgc ctgatcattt agcgctcaga atgcgcccca aaaccatttc agaggtcatc
    12781 ggacaaaaac accttgttgg cgaaggcaaa atcatccgtc gcatggtgga agcaaataga
    12841 ctatcgtcca tgatcctcta tggccctcca ggaatcggta aaacttccat tgcttctgct
    12901 attgctggta ctactagata tgccttccgg acctttaatg ccaccattga cagcaaaaaa
    12961 cgtctccaag aaattgcgga agaagctaaa ttttctggtg gcttggtatt attactagac
    13021 gagattcatc gccttgacaa gacaaaacaa gattttcttt tacccttact tgagaacggc
    13081 accattatca tgattggcgc aaccactgag aatcccttct tttcagtgac acccgctatc
    13141 agaagcaggg tccagatttt tgaattggaa ccactttcta acgaggatat taaaacagcc
    13201 attcagctag caatttccga caaagaacgg ggatttcctt ttttagtgac aattgatgat
    13261 gaggcgctag atttcattgt taccgccaca aatggagacc tacgatctgc ttataattcc
    13321 ttggatctag cagtcatgtc tacttctcct aatgaagatg gtagccgtca tattagtcta
    13381 gagacaatgg aaaatagtct gcagcgtagt tatattacca tggataaaaa cggggacggg
    13441 cattatgatg ttttatctgc cttacaaaaa tccattagag gttctgatgt caatgctagc
    13501 ctgcactacg ccgctcgttt agtagaagct ggagacttgc ctagtttagc aagacgattg
    13561 accattattg cttacgaaga tattggctta gccaaccctg acgcccaagt tcataccgtc
    13621 accgcactag atgccgctca acgcattggt tttccagaag ctcgtattct tatcgctaac
    13681 gtggtcattg atttggctct atcacctaaa tcaaattcag cttatttagc tatggatgca
    13741 gctttagctg atttgagaac atcaggtaac ctccccattc caaggcattt gagagatggg
    13801 cattatgctg gcagtaaaga actgggcaat gccaaagatt acctctaccc tcacgcctat
    13861 cctgaaaaat gggtcaaaca acaataccta ccagataaat tagtaggaca tcattatttt
    13921 gaagccaatg aaaccggtaa atacgaacgc gcacttggtt caaataaaga gcgtattgac
    13981 aagctctcag actgactgct agttttaaaa atcgcaaccg atttcaaaaa tcgataattt
    14041 tgcttgaaaa aattttaaaa agtggtatta taaaagtaca gaaacattgc tgtggcatac
    14101 gaatccacat ctaagtgttg accgactatt tctttgtatt ttcgggaaac aaagactctt
    14161 tctagtctgc aagccgtttc acgcggacgc aacttccaag aaagagcgcg ttacccacct
    14221 gcttatactg cgcgggctca atacaaaaac gtggatcacc ggtgccaata cagcattctt
    14281 gcctccttag ctcagttggt agagcagtag actcttaatc tatgggtcac aggttcgagc
    14341 cctgtagggg gtatacaact atgcatgaaa aaacctttgt ttaaaggctt tttttgcttg
    14401 tcagaagagg atttgttcca tcatttatcc ccgcagtaat ttttttatgg tatgatacca
    14461 attaaagcgc ttaagattaa ggtggagact gttatgaaaa taattgagta caaaggaaat
    14521 tacagagatg acctcatttt tatggtcttg gaagcaaaaa atgccttaag gagaattccc
    14581 agcattaacc cggatctttt gacaattgaa acaagttatt tggaaaaagg ggattgtttt
    14641 tggctagcgg tcaatgaaga aagtcgtgtt attggctcaa tcgcttattc ttctattccc
    14701 aaaacaaatg aggtcttctt gcaccgtctc ttcatcaaac caagcttaaa acatcaaggc
    14761 attggcagtc aacttctgac gtttgcagaa gattatctaa ggaaaatcgg caagagtcat
    14821 gttcgtatcc acttaggtca acctaaagaa caatggtttg agtcttatcg gttttatcct
    14881 aaacatggtt atcaatttta tgatgacacg catctcatca agaaactaat ctaaacacag
    14941 ttcataccct ttatagtcat ttcatacgtc atttttagga ggtatccatt gaaaattgcc
    15001 atcattggac atagtggctc tggaaagtca accttagcac gatttttagg ccaacattac
    15061 cattgtgagg tattccattt agaccaacta cacttctcat cgaactggca agaacggtca
    15121 gatcatgaca tgatagctga tttatcaaca tgcctgctca aacaagattg gattattgag
    15181 gggaattatg ccaattgtct ctatgaagag cgcatgagtg aagctgatta cattatctat
    15241 gttaatttca gtcggtttca ttgtgtctat cgagcattta agcgttattt aaactatagg
    15301 ggaaaaacgc gtcctgatat ggctgacaac tgtcaggcaa aatttgacgt tgcgtttgtg
    15361 aaatggattc tactagatgg acgttcaaga aaccaattaa aaaaatacca atccgttgtc
    15421 caaaaatact ctcataaaac cattgtctta acaaattaaa agcagctaag tcattatatg
    15481 aatacgatca actaattaga gtgcaaaact aaaataacca cgggtaatcc gggctattca
    15541 gactgaaagc ctttgtttaa aggctttttt gcttgtttca taactaatcg gcttaattaa
    15601 aagcgtcaat ccacctttag tatcatgtct caattttcat ttgatagtca gcaagtatat
    15661 ctacaaaatt gttaatgatt atttcttttg aaagtgttat attaaaacat aataaaataa
    15721 taactcctga aaagaggcta tttatgaaaa aaacaaaact tattttttct tttacttcaa
    15781 tattcattgc aataatttct cgtcctgtgt ttggattaga agtagataat aattcccttc
    15841 taaggaatat ctatagtacg attgtatatg aatattcaga tacagtaatt gattttaaaa
    15901 ccagtcataa cttagtgact aagaaacttg atgttagaga tgctagagat ttttttatta
    15961 actccgaaat ggacgaatat gcagccaatg attttaaagc tggagataaa atagctgtgt
    16021 tctccgtccc atttgattgg aactatttat caaaaggaag agtcacagca tatacgtacg
    16081 gcggaataac accctaccaa aaaacttcaa tacctaaaaa tatccctgtt aatttatgga
    16141 ttaatggaaa gcagatctct gttccttaca acgaaatatc aactaacaaa acaacagtta
    16201 cagctcaaga aattgatcta aaggttagaa aatttttaat atcacaacat caattatatt
    16261 cttctggttc tagctacaaa agtggtaaat tagtttttca tacaaatgat aattcagata
    16321 aatattctct cgatcttttt tatgtaggat atagaga
//
LOCUS       DAUDYX010000046        16207 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.046, whole genome shotgun sequence.
ACCESSION   DAUDYX010000046 DAUDYX010000000
VERSION     DAUDYX010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16207
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.046"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..183
                     /locus_tag="VHD02_001482"
     CDS             <1..183
                     /locus_tag="VHD02_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEQ5498080.1"
                     /translation="DSLTRQVKNIYFLQNTKDDMLSFSDINVMPRTQDIEREFNVKEW
                     IGDGIEHIENPLIEWI"
     gene            448..885
                     /locus_tag="VHD02_001483"
     CDS             448..885
                     /locus_tag="VHD02_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEQ5498081.1"
                     /translation="MGNIPTTKANNFLEELKTIPHLIETLERDANLMSRSLVKSPQWS
                     DMRVSGGVKQSQEDKNIKMLHMVSYYSDQIERLKDRRQEMANLIVQSMGICESHVLLT
                     TYLDCDGDYERARERLNIGNRNKYFMFVRRGKESLELILKNTN"
     gene            1047..2213
                     /locus_tag="VHD02_001484"
     CDS             1047..2213
                     /locus_tag="VHD02_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEQ5498082.1"
                     /translation="MEFVDKKLSEITPYKNNPRNNDEAVGPVAESIKEFGFKVPIVVD
                     KNGEIVNGHTRYKAAQKLGLETVPVIVADDLSEEQIKAFRLADNKVGEIAVWDLDLLN
                     EELNDILDLDMSAFGFDVLDNLDDLIEDEKDLDDFTGTVPDEPKSKLGDIYQLGSHKL
                     MCGDSTNGADVKKLMNGELADLLLTDPPYNVAYEGKTKDSLTIKNDSMDNDSFRQFLV
                     NAFSSANEVMKPGAVFYIWHADSEGYNFRGACFDIGWTVRQCLIWNKNSMVLGRQDYH
                     WKHEPCLYGWKDGAGHLWASDRKQTTVIDYEKPQRNGVHPTMKPVGLFDYQIKNNTKG
                     SDIVLDLFGGSGTTLIACESNGRHARLMEYDPKYVDVIIKRWEELTGESVIQLN"
     gene            2556..3032
                     /locus_tag="VHD02_001485"
     CDS             2556..3032
                     /locus_tag="VHD02_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498083.1"
                     /translation="MANEDNLIPNSKRTPSELREIAKKGGIASGKARREKANLRKAVE
                     LVLGSTVPSALLREQLEQLDISPTNQSAIALKLVENALKGDVRSAELLAKITTTEVKD
                     SLDRKEQRQRIKAAELATDEQRTRIELLKVKLDAEKGAKPDTSLMRALLDAVEGGD"
     gene            3115..4326
                     /locus_tag="VHD02_001486"
     CDS             3115..4326
                     /locus_tag="VHD02_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEQ5498084.1"
                     /translation="MTPRSGKTTAGHFRYARYLIESEDENHLVTAYNQEQAYRLFIDG
                     DGTGLMHIFDGNCEIKHDERGDHLLITTPKGNKRVYYKGGGKVNSVGAITGMSLGSVV
                     FCEINLLHMDFIQECFRRTWAAKLRYHLADLNPPAPQHPVIKDVFDVQNTRWTHWTMD
                     DNPILTAERKQNIINSLKKNPYLYKRDVLGQRVMPQGVIYGLFDTEKNVLDALIGEPV
                     EMYFCADGGQSDATSMSCNIVTRVRDNGRISFRLNRVAHYYHSGADTGQVKAMSTYAL
                     ELKVFIDWCVKKYQMRYTEVFVDPACKSLREELHKLGVFTLGAPNNSKDVSSKAKGIE
                     VGIERGQNIISDGAFYLVNHSEEEYDHYHFLKEIGLYSRDDNGKPIDKDNHAMDEFRY
                     SVNVFVHRYYN"
     gene            4340..5842
                     /locus_tag="VHD02_001487"
     CDS             4340..5842
                     /locus_tag="VHD02_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEQ5498085.1"
                     /translation="MGVIQKIKNLVTRSKYVMTTQSLTNITDHPKIAISKLEYDRITT
                     NLKYYKSDWDSVLYLNTDGETKKRDLNHLPIARTAAKKIASLVFNEQAEIKVDDDAAN
                     EFISETLKNDRFNKNFERYLESCLALGGLAMRPYVDGDKVRVAFVQAPVFLPLQSNTQ
                     DVSSAAVVIKSVKTINGKEVYYTLIEFHEWQSSDDYVISNELYRSDDKAKVGSRVPLS
                     EVYKDLKDEAKVTDVTRPIFTYLKTPGMNNKDINSPLGLSIFDNAKTTIDFINTTYDE
                     FMWEVKMGQRRVAVPESLTALTVRTADGDVVPRPRFESDQNVYIRMGGRDLDSSAIQD
                     LTTPIRADDYIKAINEGLSLFEMQIGVSAGLFSFDGKSMKTATEIVSENSDTYQMRNS
                     IVTLVEQSLKELVISIFEIAKAYDLYQSEVPSMDNISISLDDGVFTDRDAELDYWIKV
                     VNAGFGTREMAIQKVLNVTEEKAQEIAAEINTGIVDEINQQRTDTHLYGE"
     gene            5847..7340
                     /locus_tag="VHD02_001488"
     CDS             5847..7340
                     /locus_tag="VHD02_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor capsid protein"
                     /protein_id="HEQ5498086.1"
                     /translation="MKKKPIKLNDEQLLLEASQLSDMYHQLTLDLFDQVIERIKARGS
                     ASLADNPYLWQANKLHDVGLLNADNIKLIAKYSGVAEAQLRHVIENEGFKIYKNTSEQ
                     LEEALGRESQVISNIQDDLSNYARQAIDDVHNLTNTTLPFSVIGAYQGIIQDAVAGVV
                     TGLKTPDQAINQTVIKWFKKGFYGFTDKAGRKWRADSYARTVINTTTWRVFNEAKEAP
                     AREFGIDTFYYSKKATAREMCAPLQHQIVTTGEAREEGGIKILALSDYGHGEPDGCLG
                     INCKHTKTPFVVGVNSKPELPEHLKNITPAQAKANANAQAKQRAIERSIRKSKELLHV
                     AKQLGDKELIRQYQSDVRSKQDALNYLINNNAFLHRNQAREKRYNNPYTKTQSEVEVR
                     KEKAKLDKRRDVESAIIGVETSEGIPLKITKHLAERAVLRNIAPIDIVDSIKEPLKIA
                     PIKYDNLDRPSQKYIGKCVSTVINPIDGNIVTVHATSTRIRKKYGGN"
     gene            7340..7567
                     /locus_tag="VHD02_001489"
     CDS             7340..7567
                     /locus_tag="VHD02_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498087.1"
                     /translation="MKLKSILNDSQIDFVKNELPGLPVDIDVNSEKYDVFCEGIETYY
                     QTESFDEKYNITAKGKLAESIIDLLTDKGYW"
     gene            7654..7920
                     /locus_tag="VHD02_001490"
     CDS             7654..7920
                     /locus_tag="VHD02_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498088.1"
                     /translation="MNKRIKKKRKLETAVVMLVAENAMQVEAIKNQNKQIMELKSIVQ
                     RNALATNEELATVKAATLDNQSVIKAIGDTVDYIKKNYKRKWGK"
     gene            8046..8660
                     /locus_tag="VHD02_001491"
     CDS             8046..8660
                     /locus_tag="VHD02_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498089.1"
                     /translation="MHMENVTNENVDQETTDLENNQQEDKAFTQDDLNRVGTKEHSKG
                     YNKAVKDLGFDDVESAKDALKAFQEWQESQRSESEKQTEILNAKDKELEEARANNKAL
                     NAKLAAMSLGVNAESIDDVIALSERLVTDETSIEDAIKTVLGKYPHFGQTKDKAPKIT
                     VAGNPSADNGQGSVSKEDFAKMSYQEKLNLKLKNKSLYDQLKGN"
     gene            8664..9482
                     /locus_tag="VHD02_001492"
     CDS             8664..9482
                     /locus_tag="VHD02_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HEQ5498090.1"
                     /translation="MAVGTTKMAQMLDPEVLADMIDAEVGKAIRFAPLAEVDTTLEGQ
                     PGTTLTVPKWDYIGDAEDVAEGEAIPMTQLGFKKTTMKIKKAGKGVEITDEAILSGYG
                     DPVGQAAKQIVEAIDHKVDADVLTALSGSTQTVSAPASVEGVSQALDIFNDEDDAETV
                     IVMNPADASTLRLNATKEWLGATEVGANRVVSGVYGEVLGVQIVRSRKCPKGTAYMVR
                     KGALRIMLKRNTMVETDRDITKAINQIVANKHYGVYLYKAEKAVKITLKDAAKK"
     gene            9536..9952
                     /locus_tag="VHD02_001493"
     CDS             9536..9952
                     /locus_tag="VHD02_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498091.1"
                     /translation="MEVVIIAFLTQKEFEDLGFDDVEDFEKMEKRASHAVNLYCRNRY
                     DYKDLKKEIALVQKAVKRAIAYQIAYLNDSGVMTAEDKQSFAGISLGRTSISYTVGHG
                     QGSQQKTLADRFNLCLDAENELLVVGLGYTGISYDR"
     gene            9942..10274
                     /locus_tag="VHD02_001494"
     CDS             9942..10274
                     /locus_tag="VHD02_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEQ5498092.1"
                     /translation="MIDKRLLIDELQVKLVKDKGDYGGFVYDEPFTLSPVRFDRNLAT
                     AGKDNARQETKPSVIFIYPKYCKTVADRSWVDAVVIDGDTEYTVDKVIPVYHPLTNKI
                     FCFEVEVI"
     gene            10274..10630
                     /locus_tag="VHD02_001495"
     CDS             10274..10630
                     /locus_tag="VHD02_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEQ5498093.1"
                     /translation="MAKVVVELGGIKRKVSPQALAKGKLIMNNQVMMSMNPYVPYRDG
                     ALRGSSRANSVGVTWSGPHARAQFYGGAYNKYKSFKFKKYTTPGTGKRWDKRALANAT
                     IVKDWEKSLLRGMGFK"
     gene            10627..11022
                     /locus_tag="VHD02_001496"
     CDS             10627..11022
                     /locus_tag="VHD02_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEQ5498094.1"
                     /translation="MTNDFATVLRQFVEGLDLGIKPRLDYLTRQEDLAIYPMPGGKVN
                     NEYMDGTREISLPFEIAIKTKNQELASTVMWTINSALSNFDLKLPSLNHSYTFISLDV
                     EKPFLNDLSDQGFYIYVLDITAHLENRRE"
     gene            11026..11511
                     /locus_tag="VHD02_001497"
     CDS             11026..11511
                     /locus_tag="VHD02_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ5498095.1"
                     /translation="MRQKNALRGHFIAPYVKGEEKTEVTKEKLLELARWIKDISDDTD
                     EKTEDEAYYDGDGTEETTVVGVKGAYTFEGTYDPEDKAQAHIASLKYKLGDERKVWHL
                     IVSADGKTQWLGVATVTEIIAGSGAAADFEAFGCKITYNSLPKESKEIIPKKNELSVA
                     M"
     gene            11550..11984
                     /locus_tag="VHD02_001498"
     CDS             11550..11984
                     /locus_tag="VHD02_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498096.1"
                     /translation="MSGIVVDLKRTGFPVKIGQVELWFDTSQERLIEFFDIETEVNRR
                     LNEYEKQVIEANLDNEIEGKGVTKDVAQSALDLEAKYLEINYDLLFGEGTFAQLYAEY
                     PDKEALENTLEIVCREIEVKLKELAIEREKIVKQKTKKYKKE"
     gene            11988..12569
                     /locus_tag="VHD02_001499"
     CDS             11988..12569
                     /locus_tag="VHD02_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage Gp15 family protein"
                     /protein_id="HEQ5498097.1"
                     /translation="MKLNDPLVESFEFRGEIYPIDLSFNKVLDVFDVIDDDFLNEAEK
                     CFLCLDILLDRTDLPFTYAVDLWVYIKTNFIDAERPEKPQLDIKGNPMPVVKEKEDNK
                     KVIDLSLDAEFIYASFRQAYQINLLKEQNRLSWIEFKALLNALPDDTVMQRIIAIRQW
                     EDDGEGSKKYRDNMRKLKAKYSLDDGEEEDDGS"
     gene            12559..15819
                     /locus_tag="VHD02_001500"
     CDS             12559..15819
                     /locus_tag="VHD02_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEQ5498098.1"
                     /translation="MAADGKVTILVDVDGKQVKVLNSELDKVAKHGDKGSSSLKKFAV
                     GAGVFKLASTAVDLVSQSLGKAITRFDTLEKYPRVMKAMGHSAEDVARSTDKLANGID
                     GLPTTLDEVVGTAQRLTSITKDINKSTNLTLALNNAFLASGASSEAASRGLEQYAQML
                     SAGKVDMQAWKTLQETMPYALQQTAEAFGFAGASAQKDFYEALKNGQITFDQFSNKLI
                     ELNDGVGGFAELAKENSKGIETSFNNIKNAIAKGVANSIKALDDLSKAATGKGIADHF
                     DSLKVVINASFSAINASIKASTPLFKLLFSVIGAGISVVKALSPALVGVASGLAAMRA
                     VNETITMIKALNRAWVMASASMSIGATTIKTVTAVQAVSTTMTKADMVARLSQLGVLK
                     ASTVIYGVMTGAISLSTAATIAGTAAVTALKAALVALTGPVGWVVGAIGALVAVGVSL
                     WSWLTKESDETKKLKKEQEGLVESNKQLRDSVREGVQERKKGLESVKESTAAHQKLAD
                     EIIKLAAKENKTAGEKRNLKNKIDELNGSIDGLNLAYDKNSNSLSHNADQIKSRISAM
                     EAESTWQTAQQNLLNIEQKRSEVSKKLAENAELRKKWNEEANVSDSVRKEKIAELTEE
                     EAKLKNMQTQLQEEYNKTSATQQAAADAMAAAEESGSARQVIAYENMSEAQRTAIDNM
                     RTKYSELLETTTSIFDAIEQKTALSVDQMNANLEKNRAATEQWATNLEILAQRGVDQG
                     ILEQLRRMGPEGATQTQVFVDATDAELAPLQENFRAATETAKNAMGSVLDSAGVEMPE
                     KVKGMVTNVSTGLQTELQAANFAQLGQEIPNGVSQGISQGAGKASDASVKMGQEVKRS
                     FQGELGIHSPSRVFTEYGGHITDGLSNGVTNGTSKVMQTMQSLAQQMSQKGQQIVNDM
                     RSKSNQITDAFSTMSGPMHSHGVNAMQGLANGIYAGSGAALAAAQSIAARITATIQSA
                     LDIHSPSRVMRDEVGRFIPQGIAVGIDADRKVIDSSMQKLKESMTINATPEIASGFGG
                     GVAGIANKITNNSNNSFTLNVKVDESDGNSREKYQRLFREFSWYIQQQQGRLGDVK"
     gene            15816..>16207
                     /locus_tag="VHD02_001501"
     CDS             15816..>16207
                     /locus_tag="VHD02_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ5498099.1"
                     /translation="MTAFIKFDGKKSSDFDLRIINDVEHDSSFYDVDQVKVPGRDGVV
                     LKDNKRLEAIERSYPLRLYSKRRLTEVETDISNWLNVKGWKDLELSWEPDYIYKATHI
                     TPFSIKEVLRNFGRLKINFLIHPIKYLKT"
BASE COUNT         5481 a         2805 c         3695 g         4226 t
ORIGIN      
        1 gactctttaa caagacaagt taaaaacatt tattttttgc aaaatacaaa agacgacatg
       61 ctgagtttta gtgatattaa cgttatgcca aggactcaag atattgagcg agaatttaat
      121 gtcaaagagt ggattggtga tgggatagaa cacattgaaa atccactaat tgaatggata
      181 taaccaataa cggtatcccg cacgttcgac tcgtgcggtt ggtattgacc gaaattgaaa
      241 aatagaaacg aggacctcct tacaccaaaa caaaatctaa cgctggttat cggtcactgg
      301 ctattattta aggcgctaat actgacatcg tacgcctgtg tcaaaaaaca aaggaaagag
      361 aggacttttc tccacaaaaa aagacgtcca tgcgaacgcc tccatgatta aatacccaac
      421 aatattatat catgaatgga gagttagatg ggcaacattc cgacaacaaa agctaataat
      481 tttttggaag aattaaagac tatcccacat ctcatagaga cccttgaaag agacgctaac
      541 ctaatgagtc ggtcgcttgt taagtctcct cagtggtctg atatgcgagt gtctggtggg
      601 gttaaacaat cacaggaaga caaaaacata aagatgctgc atatggttag ctattatagt
      661 gatcagattg agcgtttaaa agaccgtcga caagaaatgg ctaatttgat tgtgcaaagc
      721 atgggcattt gtgagagtca cgttttactc acgacttatc ttgactgtga tggagactat
      781 gagagagcca gagaacgctt aaacatagga aatcgtaata aatactttat gtttgttagg
      841 cgaggaaagg aaagtctgga attgatacta aaaaatacta attagataca aattgatact
      901 acataatact aatagctgtg ttaatattgt agtatagcaa aataacaaga agagataacc
      961 ttttaaccaa tttgctattt atctatggtt aaccaattag tcatcacatt gtggtgactt
     1021 tttattatgt aaaaaaggaa taaaacatgg agtttgtgga caaaaaatta agtgaaataa
     1081 caccttataa aaataaccct agaaacaatg atgaggctgt tggaccagtt gctgaatcta
     1141 ttaaagagtt tggttttaaa gtgcctattg tcgttgataa aaacggcgaa atcgtaaacg
     1201 gccacactcg ttataaagca gcccaaaaat tagggctaga aacagttcct gttattgtcg
     1261 cagatgactt atctgaagag caaattaaag cgttcagact tgctgacaat aaagttggtg
     1321 agattgcggt ttgggattta gatttgttaa acgaagaatt aaacgacatt ttagacctcg
     1381 atatgtctgc ttttggattt gacgttttag ataatttaga tgatttaatc gaagatgaaa
     1441 aagatttaga cgattttaca gggactgtgc cagatgagcc aaagtcaaaa cttggtgaca
     1501 tttatcaact tggtagtcac aagttgatgt gcggcgacag tacaaatgga gccgatgtta
     1561 aaaaacttat gaatggagaa ttagctgatt tactacttac agacccacca tacaacgtgg
     1621 cttatgaggg taaaacaaaa gatagtttaa ctataaaaaa cgacagtatg gataatgaca
     1681 gttttagaca gttcttggtt aatgctttta gttctgcaaa tgaagttatg aagccaggcg
     1741 ctgtttttta tatttggcat gctgattcag aaggctataa ttttagaggt gcttgttttg
     1801 atatcggttg gacagtacgt caatgcttaa tttggaataa aaactcaatg gttctcggtc
     1861 gccaagatta tcattggaag cacgaacctt gtctttatgg ctggaaagat ggcgctggtc
     1921 acttgtgggc ttcagacaga aagcaaacaa cagttattga ttatgagaaa cctcaacgaa
     1981 atggcgttca tccaacaatg aaacctgtcg gactttttga ttaccaaatc aaaaacaaca
     2041 caaagggaag cgacattgtc cttgatttgt ttggtggctc tgggacaacg ttaattgctt
     2101 gtgagtcaaa tggtcgtcac gctagattaa tggaatatga cccaaaatat gtagatgtca
     2161 ttataaaacg ctgggaagaa ttaactggag aaagtgttat acaactaaac taaaaatccc
     2221 ttaaaaccgt tgacttaaca gtgtttcaga gctatcatgt gatcacaaag acaaagaggt
     2281 aaataacatg ctagtaacaa aagtaattca caaaatctaa aatattgaac caagcgaaaa
     2341 agattcggcc ttaaaatata gatacgccaa cgaacttgag tatctcgttt acaatctgtt
     2401 ttatagcgca attaaagaaa taactgcaaa agatatcaaa aataactttt tttatcaaga
     2461 tgatcaagcc tttattatta attggctagg actataaaat aattttaaaa gggcgaaagc
     2521 ctttttttta tatttaaaag taaggaagtg aggcgatggc aaatgaagat aatttgatac
     2581 caaactccaa acgaaccccg agcgaactcc gagaaattgc aaaaaaagga ggcatagctt
     2641 ccggcaaagc taggagggaa aaagcgaatt tgagaaaagc tgtggagctg gtgttaggat
     2701 caactgtacc aagcgccttg cttcgagaac aattagaaca attggatatt tcccccacaa
     2761 atcaatcagc tattgcactc aagcttgtgg aaaatgcttt aaagggtgat gttagatctg
     2821 cagagctatt agctaaaatt acaactaccg aggtcaaaga tagcctggat agaaaagaac
     2881 aaagacagcg aattaaggcc gcggagctcg ccacagacga gcaacgaacg cgcatcgagc
     2941 tgcttaaagt caaacttgac gcagagaagg gcgctaagcc tgatactagc ttaatgagag
     3001 ccttgttaga tgctgtggaa ggaggtgatt agccttggag attatctttt caaataaaca
     3061 aaaagacgtc attagacgcc cttttaacta cgaattagag gtcaacgagg gcacttgacc
     3121 cctcgtagcg gaaagaccac agctgggcat tttcgttatg ctaggtatct gattgagtca
     3181 gaagatgaaa atcaccttgt gactgcttat aatcaagaac aagcttatcg tttgtttatc
     3241 gacggcgatg gtacgggttt gatgcatata tttgacggta actgtgaaat aaaacacgac
     3301 gagcgtggag atcacttgtt aatcacgaca ccaaaaggca ataagcgcgt ttattataaa
     3361 ggcggcggta aagttaacag tgttggtgct attacaggta tgtctttagg atcagtagta
     3421 ttctgcgaga ttaacttact gcacatggat tttatccagg agtgttttag gcgtacttgg
     3481 gcggctaagc tacgttatca tctagcagat ttaaatcccc cagcacctca acatccagta
     3541 attaaagatg tctttgatgt tcagaacacg aggtggactc attggaccat ggatgataac
     3601 ccaatactaa ccgcagagcg taaacaaaac attatcaaca gtcttaaaaa aaatccatat
     3661 ctatacaaac gagatgtact tggacagcgg gtcatgcctc agggagttat ttatggcctt
     3721 tttgacacgg aaaaaaatgt tttggatgct ttgattggcg aaccagtaga gatgtatttc
     3781 tgtgcagatg gaggtcaatc agatgccacc tctatgtctt gtaatatcgt aacaagagtt
     3841 agagataacg gtaggataag cttcagactt aatcgtgtag ctcactacta ccacagcgga
     3901 gctgacactg gccaagtaaa agctatgtca acctacgctt tagagttaaa agtttttata
     3961 gactggtgcg ttaaaaagta tcagatgcgc tatacagagg tatttgtgga tcctgcctgt
     4021 aaatctttga gagaggagct gcataagtta ggagtattta ctctgggagc tccgaacaat
     4081 tctaaagatg tatctagcaa agcaaaaggt attgaggtcg gtatcgaacg cggccaaaac
     4141 attatctcag atggcgcttt ttatcttgtt aatcatagcg aagaagagta tgaccactac
     4201 cactttttaa aagagatagg gctgtacagt cgtgacgaca atggcaaacc tattgataaa
     4261 gataaccatg ccatggacga gtttagatac agcgtcaacg tgtttgtgca tcggtattac
     4321 aactaaagga gttgcttaaa tgggagtaat ccaaaaaata aaaaatcttg ttacaaggag
     4381 taaatacgtg atgacaacgc agagtcttac aaatataact gatcatccta aaatagctat
     4441 cagtaagcta gagtacgatc gtataacaac caatctaaag tattataaga gcgattggga
     4501 tagtgtttta tacttaaaca cggacggcga gactaaaaaa agagatctta accatctacc
     4561 aattgcacgg acagctgcta aaaagattgc cagtctagtc tttaacgagc aggcagagat
     4621 taaggttgat gatgatgcgg ctaacgaatt tattagtgag acactaaaaa acgacaggtt
     4681 taataaaaac ttcgagcggt atctggagag ttgtttagcg ttaggcggat tagctatgag
     4741 gccttatgtg gatggtgata aagttagggt agcatttgtt caagcgcctg tttttttgcc
     4801 gctgcagagt aatacgcaag acgtttcgag tgccgccgtc gtcattaagt ccgttaagac
     4861 aatcaacggc aaagaggtct actatacctt gatagagttc catgagtggc agagctctga
     4921 tgattatgtt atctcaaatg agttatatcg ctcggatgat aaagccaaag taggtagccg
     4981 agtgccgtta tctgaggtat acaaggactt aaaagacgag gcaaaagtta cagatgtgac
     5041 taggcctatc tttacttatc tcaagacccc tggaatgaat aacaaggata ttaatagccc
     5101 acttgggcta tctatctttg ataacgccaa gacaacgatt gactttatca atacgaccta
     5161 tgacgaattt atgtgggaag ttaagatggg tcaacgtcga gttgctgtgc cagagagttt
     5221 gaccgcttta actgttcgta ccgctgatgg cgatgttgtt ccaaggcctc ggttcgagtc
     5281 tgatcaaaac gtttatatcc gtatgggcgg cagggattta gactcgagcg caatacagga
     5341 cctaacaacc cctattagag ctgatgacta tatcaaggct atcaacgagg gcttgtcgtt
     5401 gtttgaaatg caaataggcg tatccgctgg gttatttagt tttgatggca aaagcatgaa
     5461 gactgcaaca gaaatcgtct ctgaaaactc agacacctac caaatgcgta acagtattgt
     5521 tactttagta gagcaatcgc taaaagagtt agttatctct atttttgaga ttgctaaagc
     5581 ttatgatttg taccaaagcg aagttccaag catggataac atcagcataa gtcttgatga
     5641 cggtgttttt acagatcgag acgctgagtt agactactgg ataaaagttg ttaatgctgg
     5701 ctttggcact cgtgagatgg ccatccaaaa agtgctaaac gtgacagagg aaaaagccca
     5761 agaaatagct gcagaaatta atactggaat cgttgacgaa atcaatcaac agcgcactga
     5821 tacacattta tacggagagt gattagatga aaaagaagcc tattaagtta aatgacgaac
     5881 agcttctttt ggaagctagt cagttatctg atatgtatca tcagctgact cttgatttat
     5941 ttgatcaagt gattgagagg ataaaagcca gaggctcagc gagcttagcc gataatcctt
     6001 atctttggca agctaataag ttacatgacg ttggactgct taatgcagat aacatcaagc
     6061 ttattgcaaa gtattctggc gttgctgaag ctcaacttcg tcatgtcatc gagaacgaag
     6121 gattcaaaat ttataaaaat acatccgaac agctagaaga ggctttgggc agagagtcac
     6181 aagttatcag taatatccaa gatgacttat ctaactatgc cagacaggct attgatgatg
     6241 tgcataattt gactaacacc accttgccat ttagtgttat aggagcttat caagggataa
     6301 tccaagacgc tgttgctggt gtggtgacag gcttaaaaac gcctgaccaa gctatcaatc
     6361 aaactgtgat taaatggttt aaaaaggggt tttatggttt tacagataaa gctgggagaa
     6421 agtggagagc agactcttat gctcgtaccg ttatcaatac tacgacttgg cgagtcttta
     6481 acgaagccaa agaagcccct gctagggagt ttggcattga taccttctat tactcaaaaa
     6541 aagctacagc tagagagatg tgtgcacctt tgcaacatca aattgtcact actggcgaag
     6601 cgagagaaga aggagggata aaaatcttag ctttatctga ttacgggcat ggtgagcctg
     6661 atggatgctt gggaatcaac tgcaagcaca ctaaaacgcc gtttgtcgtc ggtgtgaata
     6721 gtaagccaga attgccagag catctaaaaa atatcactcc tgcacaagct aaagctaatg
     6781 cgaatgcgca agctaagcag agggcaatcg agagatcaat acgtaagagt aaagagctac
     6841 tgcacgttgc gaagcaattg ggtgataaag agttgattag gcaatatcaa tcggatgtta
     6901 gaagtaaaca agatgcactc aattatctga taaacaacaa tgccttttta catcgcaatc
     6961 aagccagaga aaagcgttac aataatcctt ataccaaaac tcaaagtgaa gtcgaagtta
     7021 gaaaagaaaa agctaaatta gataaacgta gggatgttga aagtgctata ataggagtag
     7081 aaactagtga agggataccg ctaaaaataa caaagcattt agccgaaagg gcggtgctga
     7141 gaaatatagc acctattgat attgtcgatt ctataaaaga accgttgaag atagctccta
     7201 ttaagtacga taaccttgat agaccttccc agaaatacat tggtaagtgt gtctcgacag
     7261 taataaaccc gatagacgga aatattgtta cagttcatgc tactagcacg agaatccgca
     7321 aaaaatatgg aggaaattga tgaaattaaa aagtatttta aatgatagtc aaatcgattt
     7381 tgtcaaaaat gaacttccgg gattacctgt ggatatagat gttaattccg aaaagtatga
     7441 tgttttttgt gaaggtatag agacttacta ccagacagaa agctttgatg aaaagtacaa
     7501 tataacagct aaaggtaaac tagctgagag tattattgat ttactgactg ataaaggtta
     7561 ttggtgacta gtacaccaaa ctacgactta ttaaatctag agcatttagc taaaagctag
     7621 gtgctttttt gtacttaaaa aataggaggg aacatgaata aacgcattaa gaaaaaacgt
     7681 aaactagaaa cagcagttgt gatgcttgtt gcagaaaatg ccatgcaggt tgaagcaatt
     7741 aaaaatcaaa acaaacaaat catggagcta aaatcaatcg ttcaacgaaa cgctctggca
     7801 acaaacgaag agttagcgac tgttaaagct gctactttag ataaccaatc agttatcaag
     7861 gcaattggtg acacggttga ctatattaag aaaaactaca aacggaagtg ggggaaataa
     7921 agtttaaccg tgtcgaattc gaccccttta gaaatcaaag tcgtagcaat acggcttttt
     7981 attatgcctt tatccgcagg cgttaaagaa cggaaatcag cgacctatcg catttatagg
     8041 agggaatgca catggaaaat gtgacaaacg aaaatgtcga ccaagagact actgacttgg
     8101 aaaataatca acaagaagat aaagcattta cacaagatga tctcaaccga gtgggaacta
     8161 aagagcattc taaaggctat aacaaagcag ttaaagactt gggttttgat gatgtagagt
     8221 ctgccaagga tgcactaaaa gccttccaag agtggcaaga gtcacaaaga agcgagtcag
     8281 aaaaacaaac agaaatcttg aatgctaaag acaaagagct tgaggaagct cgggcaaaca
     8341 ataaggctct taatgcaaag ctagcagcaa tgtctttagg tgtgaacgct gaatctattg
     8401 atgatgtgat tgcactatct gagcgtcttg tcactgacga gacaagcata gaggatgcaa
     8461 tcaaaacggt tttgggtaag tatcctcact ttggccaaac aaaggataaa gctcccaaaa
     8521 tcacagtggc aggcaacccg tctgctgata acggacaagg ttcggtgtct aaggaagact
     8581 ttgcaaagat gtcttaccaa gaaaaactga acctaaaact aaaaaataag agtctttatg
     8641 accaattgaa aggaaattaa aaaatggcag taggaacaac taaaatggca caaatgctag
     8701 acccagaggt tctagcggat atgattgacg cagaagtagg aaaagcgatt cgctttgctc
     8761 ctcttgcaga agtagataca accttagaag ggcaaccagg taccacacta acagtaccta
     8821 agtgggatta catcggtgac gcggaagacg tggccgaagg agaagctatc ccaatgactc
     8881 aacttggatt taaaaagacc acaatgaaaa tcaaaaaagc gggtaaaggt gtagaaatta
     8941 ctgacgaagc tattttatct ggatatggcg atccagtagg ccaggcagcc aagcaaatcg
     9001 tagaagctat tgaccacaaa gttgatgcgg atgtattgac tgcccttagt ggttctactc
     9061 aaaccgttag cgctcctgct tctgttgagg gagtatctca agcgctcgac atctttaacg
     9121 atgaggacga tgctgagaca gttattgtca tgaacccagc agatgcatct actctacgtt
     9181 taaatgctac gaaggagtgg ctaggtgcta ccgaggttgg agcaaatcgt gttgtctctg
     9241 gtgtttatgg tgaagtttta ggggtacaaa ttgtgcgttc tcgcaaatgc cctaaaggaa
     9301 ctgcctacat ggttcgcaaa ggtgcactac gtatcatgct taaacgtaac acaatggttg
     9361 aaacagaccg agatatcaca aaagcgatta atcaaatcgt agctaataag cattatggtg
     9421 tttatcttta taaggcagaa aaagccgtca aaattacact taaagatgcc gcaaaaaagt
     9481 agctaaggct aaggctgcca agagagagga agatgacgct ctcgagtctt agccgatgga
     9541 ggtggttatt atcgcttttt taacgcaaaa agaatttgaa gatttgggtt ttgacgatgt
     9601 agaagatttt gaaaaaatgg aaaaacgtgc tagccacgct gtcaatcttt actgtcgtaa
     9661 tcgctatgat tacaaagatt taaaaaaaga aatagcccta gtgcaaaagg ctgtaaagcg
     9721 ggcaatcgct tatcagatag catatttaaa tgactcagga gtaatgacag cagaggataa
     9781 acaatccttt gctggaatat ctctaggacg gacaagtatt agctacactg tcgggcatgg
     9841 ccaaggcagc caacaaaaaa ctctggcaga caggtttaat ctctgtctag atgcagagaa
     9901 tgagttacta gttgttgggt tgggatatac gggtattagc tatgatcgat aaaagactgc
     9961 ttattgacga actgcaggta aaacttgtca aagataaagg tgattatgga gggtttgtct
    10021 atgacgaacc ttttacactc tctccagtta gatttgaccg taatcttgca accgcaggta
    10081 aagataatgc aaggcaagag actaaaccgt cggttatctt tatttatcct aaatactgta
    10141 agacagtagc cgataggtca tgggttgatg ctgttgtcat tgatggtgat actgagtaca
    10201 ccgttgataa agtgatacca gtctaccacc cactaacaaa caaaattttt tgttttgaag
    10261 tggaggttat ctaatggcta aggtagtggt agagttggga ggcatcaagc gtaaagtatc
    10321 tccgcaagca ttagctaaag gaaagctcat catgaacaac caagtcatga tgtccatgaa
    10381 cccttatgtt ccttacagag acggagcttt gagaggaagc tcgagagcta acagtgtagg
    10441 tgtaacgtgg agcggaccac acgccagagc ccagttttat ggtggtgctt acaacaagta
    10501 caagtccttt aaatttaaaa agtacacaac accaggtaca ggcaaacgtt gggataaacg
    10561 tgcgttagct aatgcaacaa ttgtcaagga ttgggaaaaa tccttactga gaggaatggg
    10621 atttaaatga caaatgactt tgcaactgtt ttgaggcaat ttgtcgaagg attagactta
    10681 ggcataaagc ctagacttga ctatctaaca agacaagaag acctagccat ttatccaatg
    10741 ccaggaggta aggtaaataa cgagtacatg gacggtactc gtgagataag cctgcctttt
    10801 gagattgcaa tcaaaactaa aaatcaggag ttagcaagca ctgtgatgtg gactattaac
    10861 agtgctttgt ctaactttga cttaaaatta ccaagtctta atcattcgta cacatttatt
    10921 agccttgatg tcgaaaagcc gtttttaaat gacttaagcg atcaaggttt ttatatttat
    10981 gtgctggata ttacagcaca cttagaaaat agaagggaat aactaatgag acaaaagaac
    11041 gccctacgtg ggcattttat cgcaccttat gtaaaaggtg aggaaaagac agaagttaca
    11101 aaagagaagc ttttggaatt ggctagatgg attaaagaca tctcagacga taccgacgaa
    11161 aagacagaag atgaggcgta ctatgacgga gatggaacag aagaaacaac cgttgtagga
    11221 gttaagggcg cgtacacttt cgaagggacg tatgatccag aagacaaagc tcaagctcac
    11281 attgcaagtc ttaagtataa gctaggagat gagcgaaaag tttggcattt gattgtatcg
    11341 gctgatggga aaacccagtg gctaggggtt gcaacagtga cagaaattat tgctggttct
    11401 ggtgctgccg ccgattttga agcctttggt tgcaagatta cttataactc tctgccaaag
    11461 gaaagcaagg aaattatacc taaaaaaaat gaattgagcg tagcgatgtg agacaagggt
    11521 gggctaaacc acccttttaa ggaggaatta tgtctggaat tgtagttgac ctaaagcgca
    11581 cagggtttcc ggtcaaaatt gggcaggtag agctttggtt tgatacaagc caagagcgat
    11641 tgattgagtt ttttgatatt gagacagaag taaatcgtcg cctcaatgaa tacgaaaaac
    11701 aggttatcga agccaactta gacaacgaaa ttgaaggtaa aggggttacg aaagatgttg
    11761 cccaaagtgc tttagatttg gaagctaaat acttagaaat caattatgat ctactattcg
    11821 gagaaggtac atttgcacag ctctacgcgg agtatcccga caaagaagca ctcgagaata
    11881 ctctggaaat tgtttgcagg gaaatcgagg tcaaactcaa agagttagct atcgaaagag
    11941 aaaaaatcgt taagcagaaa acgaaaaagt acaaaaagga gtagcctatg aaactaaacg
    12001 atccattagt agagtcgttt gagtttagag gtgaaatcta tcctattgat ttgagcttta
    12061 acaaggtttt agatgtcttt gatgttattg atgatgattt tttaaacgag gcggaaaagt
    12121 gttttttgtg ccttgatatt ttgctagata ggacagattt accttttact tacgcggtgg
    12181 atctttgggt ttatatcaaa accaatttta tcgatgcaga aaggcccgag aaacctcagc
    12241 tcgacatcaa agggaatcca atgcctgtag taaaagaaaa agaggataac aaaaaagtca
    12301 ttgatttgag tttagacgct gagtttatct atgcgagctt taggcaagcc taccaaatca
    12361 atcttttaaa agagcaaaac agattgtctt ggattgaatt caaggctctt ttaaatgctc
    12421 ttccagacga tactgtcatg cagcgtatca tagccattag acagtgggaa gatgatggtg
    12481 aaggcagtaa aaaatacaga gataacatgc gtaagctaaa ggctaagtac agtttagatg
    12541 acggagagga ggaagacgat ggcagctgat ggtaaggtaa cgatacttgt tgacgttgat
    12601 ggtaaacagg taaaggtgct caatagtgag ttagataaag ttgccaagca cggtgacaaa
    12661 ggcagctcct ctcttaaaaa atttgcggtt ggtgcaggag tctttaaatt agcttcgact
    12721 gcagttgatc tggttagtca atctcttggc aaggctatca caagatttga cacgcttgaa
    12781 aaatatccaa gggtgatgaa agcaatgggc catagcgctg aggatgttgc gagatcaaca
    12841 gataagctgg ctaatgggat tgatggtttg ccaacaactc tagacgaggt tgtcggaacc
    12901 gctcaacgtt tgacttctat cactaaagat atcaataagt cgaccaatct cacactagct
    12961 ttaaacaatg cctttttagc ttcaggagct tcatcagagg ctgcaagccg agggctggag
    13021 caatatgccc aaatgctatc agctggtaag gttgatatgc aagcttggaa aaccctccaa
    13081 gaaacaatgc cttatgcctt acaacaaact gcggaagctt ttggatttgc aggggcatcg
    13141 gctcaaaagg atttttatga ggcgttaaaa aacgggcaaa taacatttga ccaattttct
    13201 aataagttga ttgagttaaa tgatggtgtc ggcggttttg cagaactagc caaagaaaat
    13261 agtaaaggga ttgaaacctc ttttaacaac atcaagaacg ctattgcaaa aggtgtggcc
    13321 aatagcatta aggctttgga tgatttgtct aaggctgcaa caggtaaggg catagctgat
    13381 cattttgata gtttgaaagt tgttatcaat gcctctttta gcgccatcaa tgcaagtatt
    13441 aaagctagta caccgctatt taaacttttg tttagtgtta ttggtgctgg aatatcagtc
    13501 gtcaaagctc tgtcgcctgc cttagttggt gtagcatctg gtctagctgc catgagggca
    13561 gttaatgaga ctataacaat gattaaagcg ctaaatagag cttgggttat ggcatctgca
    13621 tcaatgagta ttggagcaac aaccattaag actgtgactg cggtacaagc ggtaagtacc
    13681 acgatgacta aagcagatat ggttgcaaga ctatctcagt taggtgtctt aaaagccagt
    13741 accgtgattt atggtgttat gacaggcgct atcagtttat ctactgctgc aaccatagcc
    13801 ggtactgctg cggtaactgc gttaaaagca gcacttgtag ccttaacagg tcccgttggt
    13861 tgggtagtag gtgcgattgg tgcgttagtt gctgttggag taagtttgtg gtcatggcta
    13921 actaaagagt cagatgagac taaaaagctg aaaaaagaac aagaagggct agttgaaagt
    13981 aataaacagc taagagattc tgtccgtgag ggcgtgcaag agcgtaagaa gggccttgag
    14041 tccgtcaaag aaagcactgc tgctcatcaa aaattagctg atgaaatcat taagttagca
    14101 gccaaagaaa acaaaactgc aggcgaaaaa cgaaatctga agaataagat tgacgagctt
    14161 aatggatcta ttgatggctt gaacttggcc tacgataaaa actcaaactc tctttcgcac
    14221 aacgcagatc aaattaagtc acgcattagc gccatggaag cagaaagcac atggcaaaca
    14281 gcacaacaaa acctgttaaa tattgaacag aaacgtagtg aggttagtaa aaagctagct
    14341 gaaaatgccg agctacgtaa aaagtggaat gaagaagcta acgtctccga ttccgtccga
    14401 aaagaaaaga ttgcagaact cacagaagaa gaagctaaac ttaaaaatat gcagactcaa
    14461 ctgcaggagg agtataacaa gacatcagct actcaacaag ctgctgcaga cgctatggct
    14521 gccgctgaag aatcaggatc agcaagacag gttatagcgt acgaaaatat gtcagaagct
    14581 caacgaactg ccatagacaa tatgcgcact aagtactctg aacttttaga gacaacgaca
    14641 tctatttttg atgctatcga acaaaagacg gcattatcag tagatcaaat gaatgccaac
    14701 cttgaaaaaa atagagctgc tactgaacag tgggctacta atttggagat tttggctcag
    14761 cgtggtgtgg atcaaggtat tttggagcaa ctaaggcgca tgggtcctga aggagccaca
    14821 cagacgcaag tttttgtgga tgcaacagat gccgagctag cacccttgca ggaaaacttt
    14881 agagcagcca cagaaactgc taaaaatgca atggggagcg ttttagactc agcaggtgtg
    14941 gaaatgccag aaaaagttaa agggatggtc actaatgttt ctacgggatt acagacggaa
    15001 ctgcaagctg ctaactttgc tcaacttggc caagaaatcc ctaatggagt ttctcaaggt
    15061 ataagtcaag gggcaggtaa agcaagtgac gcaagtgtca aaatgggtca agaagttaaa
    15121 cgctcttttc aaggagagtt gggtatccac tcgccatcgc gagtatttac tgagtacggt
    15181 ggccatatta ctgatggctt gagtaatggt gtgacaaatg gaacgtcaaa agttatgcaa
    15241 accatgcaga gcttggctca acagatgtct caaaaaggac agcagattgt taatgacatg
    15301 cgtagcaagt cgaaccaaat cacagatgct tttagcacga tgagtggtcc aatgcactct
    15361 catggtgtta atgccatgca aggtttggcc aatggtattt atgcagggtc gggggcagct
    15421 ttagcggcag ctcaaagcat tgcggcacgt atcaccgcaa caattcaaag tgccttagat
    15481 atccactcgc catctcgtgt tatgagggat gaggttggac gttttatccc tcagggtatc
    15541 gctgtaggta ttgatgcgga tagaaaagtc attgactcat ctatgcaaaa gctaaaagag
    15601 tcaatgacga ttaatgcgac cccagaaata gcctctggat ttggcggagg agttgcgggg
    15661 attgctaata agatcacaaa taactcaaat aacagtttta cccttaatgt caaggttgat
    15721 gaatccgacg gtaatagccg cgagaaatat caacgcttat tcagagaatt tagctggtat
    15781 attcaacaac aacaaggaag gttaggtgat gttaaatgac agcttttatc aagtttgatg
    15841 gtaaaaaatc ttcagatttt gatttgagaa ttattaatga cgttgagcat gactcgtcct
    15901 tttacgatgt tgatcaagtt aaggtacctg gtcgtgatgg tgtggtttta aaggacaata
    15961 aaaggcttga ggctattgaa cggtcttacc ctttacgtct atatagcaaa agacgactca
    16021 ccgaagtaga aactgacata agcaattggc tgaatgtaaa aggttggaaa gacttagagt
    16081 tgtcatggga gcctgattat atctataaag caacacatat cacccctttt agcataaagg
    16141 aggttttaag gaatttcggc agactgaaaa tcaacttctt aatccaccct atcaaatatt
    16201 taaaaac
//
LOCUS       DAUDYX010000047        15535 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.047, whole genome shotgun sequence.
ACCESSION   DAUDYX010000047 DAUDYX010000000
VERSION     DAUDYX010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15535
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.047"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            140..709
                     /locus_tag="VHD02_001502"
     CDS             140..709
                     /locus_tag="VHD02_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEQ5498100.1"
                     /translation="MGKEIKVKCFLRRSAFGLVAVSASVLVGSTVSAVDSPIEQPRII
                     PNGGTLTNLLGNAPEKLALRNEERAIDELKKQAIEDKEATTAIEAASSDALEALADQT
                     DALQSEEAEVVQSDNAASDAWEKAATPIALDVKKTKDTKPVVKKEERQNVNTLPTTGE
                     ESNPFFTAAALAIMVSTGVLVVSSKCKEN"
     gene            947..1489
                     /locus_tag="VHD02_001503"
     CDS             947..1489
                     /locus_tag="VHD02_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ5498101.1"
                     /translation="MDIWTKLAVFAFFETPKVILRPFRYEDHWDFYSMVNDTKNLYYV
                     FPEQKTKAASDYLLVHSFIKFPLGQWAIEDKATHQVIGSIRIEHYDAKTRCADIGYFL
                     NYAFWGQGIMTEVVIKLVYLSFHEFGLKTLRIITHLENKASQKVAKKAGFQLKTCFKG
                     SDRNTHKICIYKMYQLTNDR"
     gene            1498..2781
                     /locus_tag="VHD02_001504"
     CDS             1498..2781
                     /locus_tag="VHD02_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEQ5498102.1"
                     /translation="MSKHQDILDYLEKLAIGKKVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETKPRSGTVRIEKKGRVRIDRLTYSEIARISDSEVLAGHAGLGHEFSRFSIGA
                     MTQQNIRRYLVKGGLLIVGDRETIQLLALENHNAILVTGGFPVSKRVIEMANNQRIPV
                     MVTHYDTFTVATMINHALSNIRIKTDLKTVEQVMIPITDYGYLCEDSSVEEFNTLIKK
                     TRQVRFPVLDYKRKVIGVVSMRDVVDQLPTTKLTKVMSKNPITARPNTSLANISQKMI
                     FEDLNMLPVTDEENNLLGMITRRQAMENLPNHQPNNPYTYSEQILSNLEETVDYYQVV
                     VEPTMIDSAGNMSNGVISEFLKEISIRALTKKHQKNIIIEQMMVYFLHAIQIEDELKI
                     YPKIITENRRSSTIDIEIFVDDQVIAKAIITTKIN"
     gene            2797..3657
                     /locus_tag="VHD02_001505"
     CDS             2797..3657
                     /locus_tag="VHD02_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEQ5498103.1"
                     /translation="MITLKSAREIEAMDRAGDFLAGIHIGLRDIIKPGVDMWEVEAYV
                     RRRCKEDNVLPLQIGVDGHMMDYPYATCCGLNDEVAHAFPRHYILKEGDLLKVDMVLS
                     EPLDKSIVDVAALDFDNVPEMKKWTGSYTGGLADSCWAYAVGTPSDEIKQLMDVTKEA
                     MYRGIEKAVIGNRIGDIGAAVQEYAESFGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLKEGMVLTVEPMINTGTWEIDTDIKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            3659..4624
                     /locus_tag="VHD02_001506"
     CDS             3659..4624
                     /locus_tag="VHD02_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEQ5498104.1"
                     /translation="MAEKKWFDKVLSKWQYEPIQVFMRHLQSAEMDLSAIAVAYYLIL
                     TAFPLIVIAANIFPYLNIDIADLLRLMKQNLPKDIFRPASAIVENIFSKPSGSVLGVA
                     TLTGLWTMSRSLTSLQKAINKAYGASQHRDFFIGHLVGLLTSLIILFLLAFALIFSTF
                     SKAAIQVLDKHYHLSDNITTIFLLLIQPITVLIIFVGLMLLYFLLPNVKIKKIRYILP
                     GTLFTSFVMTFLSNLVGNYVVYNVERMVDIKMFGSVMIFIIMLWFIFLARILILGAIF
                     NATYQEMSLGKLEGRSGDMIAILKKTLGNDSDLSPSQSIEDSHTD"
     gene            4735..6018
                     /gene="aroA"
                     /locus_tag="VHD02_001507"
     CDS             4735..6018
                     /gene="aroA"
                     /locus_tag="VHD02_001507"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEQ5498105.1"
                     /translation="MKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLK
                     GEDVLSTIQAFRNLGVRIEEKDDQLVIEGQGFQGLTAPCQTLNMGNSGTSMRLIAGLL
                     AGQPFSVKMIGDESLSKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNRNLQPITY
                     TLPISSAQVKSAILLAALQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVG
                     PQQLTAQEITVPGDISSAAFWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQI
                     VYEDMNKKEQVTSIRVVYSHLKGTIISGGLIPRLIDELPIIALLATQAQGTTCIKDAQ
                     ELRVKETDRIQVVTDTLNSMGANIKATADGMIIKGPTVLYGANTSTYGDHRIGMMTAI
                     AALLVKQGQVHLDKEEAIMTSYPTFFKDLERLCHD"
     gene            6011..6502
                     /locus_tag="VHD02_001508"
     CDS             6011..6502
                     /locus_tag="VHD02_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEQ5498106.1"
                     /translation="MTKVLLGFMGVGKTTVSKHLSMHCKDMDAIIEAKIGMSIAAFFE
                     QHGEIAFRTIESQVLKDLLFANDNSVIVTGGGVVVLQENRQLLRKNHQHNILLVASFE
                     TLYQRLKHDKKSQRPLFLKYSKEAFYEFYQQRMVFYEGLSDLVIRVDHRTPEEVANII
                     EGY"
     gene            6710..8161
                     /locus_tag="VHD02_001509"
     CDS             6710..8161
                     /locus_tag="VHD02_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEQ5498107.1"
                     /translation="MTKYPMGGLSHHEELRYFYLLRNLSYLSENEKKEFAFLKSKLEI
                     GRAYAPSKQHYRKSKRQEPYFEDDYYNDYSPNDLLEDDDVNHDSSFVPYGLPIYPKED
                     RYLNKKTKLTARRPIDAPQPIDEDDAFLTESVARCALPRSQKRKHKKKGCMKWFFNIL
                     GLLLMTVLMGLGLMFAKGVFDISTNKANYKPAVSQAFDGQETQDGTNILVLGSDQRVT
                     QGSTDARTDTIMVVNVGNHAKKIKMVSFMRDTLINIPGYSYNDNSYDLKLNSAFNLGE
                     QEDHHGAEYVRRALKHNFDIDIKYYMMVDFETFAEAIDTLFPNGVKIDAKFATVGGVA
                     VDSVEVPDDLRMKNGVVPNQTIEVGEQRMDGRTLLNYARFRKDDEGDFGRTVRQQQVM
                     SAVMSQIKDPTKLFTGSAAIGKIYALTSTNVSFPFVVKNGVSVLGSGKNGVEHVTIPE
                     NGDWVDEYDMYGGQALYIDFDKYQKTLAKLGLR"
     gene            8210..9598
                     /gene="rlmD"
                     /locus_tag="VHD02_001510"
     CDS             8210..9598
                     /gene="rlmD"
                     /locus_tag="VHD02_001510"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEQ5498108.1"
                     /translation="MVSPRKGKRIRMLKKNDIIQVAISDLSHEGAGVAKHDGFVFFVD
                     NALPEEVIDMRVLKVNKNSGFGKVEAYHYLSPARNADVNLTYLRTGIADLGHLTYEDQ
                     LTFKKKQVQDSLYKIAGISDVTVESTIGMTEPLAYRNKAQVPVRRVNGQLETGFFRKH
                     SHDLIPISDYYIQDKEIDRLINFTRDLLRRFDIKPYDETEQTGLLRNIVVRRGHYSGE
                     MMLVLVTTRPKVFRVDQVIEKIVEAFPAVVSIIQNINDKNTNAIFGKDFKTLYGKDTI
                     TDSMLGNNYAISAQSFYQVNTIMAEKLYQTAIAFSDLSKDDIVIDAYSGIGTIGLSFA
                     KTVKAVYGVEVIEAAVRDAQQNAALNGITNAYFVADTAEHAMATWAKDGIKPSVILVD
                     PPRKGLTESFIQASVAMGPQKIIYISCNPATMARDIKRYQELGYKLTKVQPVDLFPQT
                     HHVECVVLLIKE"
     gene            9637..10431
                     /locus_tag="VHD02_001511"
                     /pseudo
     CDS             9637..10431
                     /locus_tag="VHD02_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060407.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
     gene            10516..10917
                     /locus_tag="VHD02_001512"
     CDS             10516..10917
                     /locus_tag="VHD02_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEQ5498109.1"
                     /translation="MSDLTQEMTLNVISIFDNYQIELAEKGHLILSTEVTETALNYYG
                     NAHGGYLFTLCDQVGGLVARTTGVESVTLQANANYLKAGHKGDKLMVEGRLVHGGRTT
                     QVVDVTIHNQTGALLTKISLTMFVTGRRHNG"
     gene            complement(11022..12506)
                     /locus_tag="VHD02_001513"
     CDS             complement(11022..12506)
                     /locus_tag="VHD02_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEQ5498110.1"
                     /translation="MKTIAFDSNKYLNLQRDHILERISQFDGKLYMEFGGKMLEDYHA
                     ARVLPGYEPDNKIKLLKELKEQVEIVIAINANNIEHSKARGDLGISYDQEVFRLIDKF
                     NTLDIYVGSVVITQYNNQPAADAFRKQLEKNGIASYLHYPIKGYPTDINHIISSEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSQMYHDQINGVKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLETYGKTAVNYNRDIEVFPVLNRTFERILSKSPYA
                     SPTDMGVNMVGFSIVNEEAAIEASKQEIIRRYYQTLVDFKAERVTESAVKKIELLMND
                     IGVTPDDRHVTVAAHQKAEQTGQPALALQLPNGQIVTGKTSELFGPTAAVIINAIKTL
                     AKIDKTTHLIEPEYVKPIQGLKVNHLGSHNPRLHSNEILIALAITAMTSEEANLAMKE
                     LGNLKGSEAHSTVILTEEDKNVLRKLGVNITFDPVYQYHKLYRK"
     gene            12880..14100
                     /locus_tag="VHD02_001514"
     CDS             12880..14100
                     /locus_tag="VHD02_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ5498111.1"
                     /translation="MSTLSLDTTNKRALVAAIAASGTDDLNVMFLAFSMSSIMTDLGL
                     SGTQGGWIATITNLGMLVGGLLFGLLADRHHKFKVFKWTILLFSVATGLIYFTQSLPY
                     LYLMRFIAGIGVGGEYGIAIAIMAGIVPPEKMGRMSSLNGIAGQLGSISSALLAGWLA
                     LSLGWRGLFLFGLLPILLVIWMTLAIDDQKIWDHYGQEEEECSQPIKINELFKTKSLT
                     AQTLALMVMTTVQIAGYFGMMNWLPTIIQTSLNLSVKSSSLWMVATIVGMCLGMLCFG
                     QLLDCFGPRLIYSLFLLASSICVYLFQFANSMASMVIGGAIVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNVILNVGRALGGFSSVAIGSILDASGISMVMIFLASLYVISFGAMW
                     TIGQLKAERYQQLR"
     gene            complement(14220..14585)
                     /locus_tag="VHD02_001515"
     CDS             complement(14220..14585)
                     /locus_tag="VHD02_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEQ5498112.1"
                     /translation="MTIYSHTYETRPNHSAKAMGSGTLNVLATPALVAFMENASYLFA
                     QEQLDSDFTTVGSEIAIQHLAASAIGDPVTIIITALKEEGRKYDFRLEAFVGEQLIGK
                     ACHTRVRVNSKHFMAKVAE"
     gene            14699..15430
                     /locus_tag="VHD02_001516"
     CDS             14699..15430
                     /locus_tag="VHD02_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEQ5498113.1"
                     /translation="MRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEY
                     KDLVTYQGTPLVYESFVYYLLNKPSGYVSATQDRQQATVMELLDDTARQKAVFPVGRL
                     DKDTRGLLLLTNNGQLAHDLLSPKKHVTKEYLAKVAGIMTEADKDYFARGISLKDHQC
                     LPAHLEVLASDLQQQTSLVKITIQEGKFHQVKRMVAACGKEVLDLQRLSMGPLKLDPS
                     LAEGEFRRLTPEELQSLAPYCQSSL"
BASE COUNT         4842 a         2689 c         3285 g         4719 t
ORIGIN      
        1 aaaataatat ataaattaga taataaactt gattttatgc aattaatctg tataataaaa
       61 gatggaatta ttaattataa tttccaaatt atcggtaatt taatatgcta atgcatataa
      121 aaataaaaaa ggagaaacaa tgggaaaaga aataaaagtg aaatgctttt tgcgtagatc
      181 agcttttgga ttagttgcgg tgtcagcatc agtattagtc ggttcaacag tatctgctgt
      241 tgactcacct atcgaacagc ctcgaattat tccaaatggc ggaaccttaa ctaatcttct
      301 tggcaatgct ccagaaaaac tggcattacg taatgaagaa agagccattg atgaattaaa
      361 aaaacaagct attgaggata aagaagctac gacagctata gaagcagcaa gttcagatgc
      421 cttagaagca ttggcggatc aaacagacgc tttacaatca gaagaagctg aagtagttca
      481 atcagataac gctgctagtg acgcctggga aaaagcagca actccaatcg ctttagatgt
      541 taagaaaact aaagatacaa aacctgtagt taaaaaagaa gaaagacaaa acgttaatac
      601 ccttcctaca actggtgaag agtctaaccc attctttaca gctgctgcgc ttgcaataat
      661 ggtaagtaca ggtgtgttag ttgtaagttc aaagtgcaaa gaaaattagt tgctatttgt
      721 tctagcaaat gaaacaaggg aatcgaaaga ttctcttttt ctgatattta aaaatgctac
      781 agtttataat agcttgaaag actaaatgtt ctcaaactca ttagaacaag ctttaataaa
      841 actaacccag ttgtatcaaa ggatagataa agactaagac tgttatagaa tatttgcaaa
      901 agatttgtta taatggaccc aatacaggta ttgtgaaagg tataatatgg atatttggac
      961 aaagcttgca gtgtttgctt tttttgagac tccgaaagta atattacggc cttttcgcta
     1021 tgaagatcat tgggattttt acagcatggt taacgacact aaaaaccttt attatgtttt
     1081 tccagaacaa aaaactaagg cagcaagtga ctatctttta gtacatagtt ttataaagtt
     1141 tcctttaggt cagtgggcaa tagaagataa agcaacccac caagtaatag gttctattag
     1201 aattgagcat tatgatgcta aaacgcgttg tgctgatatt ggctattttt taaactatgc
     1261 cttttgggga caaggaatta tgacagaagt cgtaataaaa cttgtttatt tatcttttca
     1321 cgaatttggt ctcaaaacgt tgcgtattat aactcattta gaaaataagg ccagccagaa
     1381 agtagctaaa aaagcaggtt ttcaactaaa aacctgtttt aaaggaagtg atcgtaatac
     1441 tcataaaatc tgcatttata aaatgtacca actaactaat gataggtgat aatcattatg
     1501 agcaaacatc aagatatttt agattattta gaaaaacttg ctattggtaa aaaagtgagt
     1561 gttagaagca tttcaaacca cttaaaagta agcgatggaa cagcctaccg tgctattaaa
     1621 gaggcagaaa atcgaggtat tgtcgaaacc aaaccaagaa gtggaactgt tcgtattgaa
     1681 aaaaaaggac gggttcgtat tgaccgtctg acctattccg aaattgctcg gatcagtgat
     1741 tcggaagtgc tagcgggaca tgctggttta ggacatgaat ttagtaggtt ttcaattggt
     1801 gctatgactc aacaaaatat tcgtcgttac ttggtcaaag gagggttatt aatagttggg
     1861 gatcgtgaaa cgattcaact cttagctctt gaaaaccata atgccatttt ggtgacgggc
     1921 ggatttccag tatccaaacg tgttattgaa atggcaaata accaacgaat cccagttatg
     1981 gtaactcatt atgatacctt tactgtggca acaatgatta atcacgcgct atctaatatt
     2041 cgtattaaaa cagatctcaa aacagttgaa caggtaatga tacctattac agattatggt
     2101 tacctttgcg aagacagctc tgttgaagaa ttcaatacat taataaaaaa aacacgacaa
     2161 gtcaggtttc ctgtattaga ttataaaaga aaagtgattg gtgtagttag tatgcgtgat
     2221 gtggtggatc agttgccaac aactaagctt accaaggtca tgtctaaaaa tccaataacg
     2281 gctagaccaa atactagtct tgctaatatt agccagaaaa tgatctttga agatttaaac
     2341 atgttacctg tcactgacga agaaaataat ttacttggta tgattactcg tagacaagca
     2401 atggaaaatt tgccaaatca ccaaccaaat aacccctata cttatagtga gcaaatactt
     2461 tctaaccttg aagaaacggt tgattattac caagtagtag ttgagccaac aatgattgat
     2521 agtgctggta acatgtcaaa cggtgttatt tcagagttct taaaggaaat tagtatcaga
     2581 gccctcacga aaaagcacca aaaaaatatc attatcgaac aaatgatggt atacttttta
     2641 catgctattc aaattgagga tgaattaaaa atatatccta aaatcattac tgaaaataga
     2701 cgaagcagca ccattgatat tgaaattttt gtggatgatc aagtgattgc taaagctatt
     2761 attacaacga aaataaatta gaggagtaac aaaacaatga taacattaaa atcagcccgt
     2821 gaaatagaag cgatggatag agcaggagac ttcttagcag gtatccatat tggcttaagg
     2881 gatattatta agcctggtgt ggatatgtgg gaagtggaag catatgttcg tcgccgttgt
     2941 aaagaagaca acgttcttcc tttgcaaata ggggttgatg gtcacatgat ggactatcct
     3001 tatgcgacct gttgtggtct aaatgacgaa gtagcacatg cctttccccg tcattacatt
     3061 ctgaaagaag gggatttgct taaagttgac atggtactca gtgagccttt ggataaatca
     3121 attgttgatg tagcagcttt agattttgat aacgtgcctg agatgaagaa atggacagga
     3181 tcttataccg gtggtttagc tgactcttgt tgggcttatg ctgttggaac accgtctgac
     3241 gaaataaagc aattaatgga tgtcaccaaa gaagccatgt accgtggtat tgaaaaagca
     3301 gtaattggca atcgtattgg tgatattggc gcagctgttc aagaatacgc tgaaagcttc
     3361 ggttatggtg tcgtgcgtga tcttgttggt cacggtgttg gtcctacaat gcatgaagag
     3421 ccaatggtac caaactatgg aacagcaggt cgtggacttc gtctaaaaga aggcatggtc
     3481 ttgactgttg agccaatgat taatacagga acttgggaaa ttgataccga catcaagaca
     3541 ggttgggctc ataaaaccct agatggtggt ttatcttgcc aatacgaaca ccaatttgtg
     3601 attactaaag atggtcccgt tattttaaca agtcaaggag aagaaagaac ttactaaaat
     3661 ggcagaaaaa aaatggtttg ataaggtact gtccaagtgg cagtatgaac ccattcaagt
     3721 ctttatgagg catttacaaa gtgcagaaat ggatttatct gccattgcgg ttgcgtatta
     3781 ccttatctta acggcctttc cattaattgt tattgcagcc aatatttttc cttacctcaa
     3841 tattgatatt gctgatttgt tacgtttaat gaagcaaaat ttgcctaaag atatttttag
     3901 accggcttct gcaattgtag aaaatatttt ctctaaaccg tctggaagtg ttttgggagt
     3961 agcgactttg acaggattat ggacaatgtc tcgaagtttg acatcgctgc aaaaagctat
     4021 taataaagcc tatggcgctt ctcagcatcg agatttcttt atcggacact tggttggttt
     4081 gttgaccagt ttaattattt tatttttatt agcctttgcc ttaatctttt caacattttc
     4141 taaagctgct attcaagtgc tggataagca ttaccatttg agtgataata tcacaacgat
     4201 tttcttgctc ctcattcaac ctattacagt tttgattatt tttgttggct tgatgctact
     4261 atatttttta ttacccaatg ttaagattaa aaaaattcgc tacattttac caggaaccct
     4321 atttacatct tttgtgatga catttttgag taatctggtt ggcaattacg ttgtgtataa
     4381 tgttgagcgg atggtagata ttaaaatgtt tggttcagtt atgattttta tcatcatgct
     4441 atggtttatt ttcttagcaa gaatcctgat tttaggagct atatttaatg cgacctatca
     4501 agaaatgtcc ttggggaaat tagaaggtcg tagtggtgat atgatcgcta tccttaaaaa
     4561 aacacttggg aatgattcag atctatctcc ttcacagtct atcgaagata gtcacactga
     4621 ttgatacaaa aaagataata agtcgtggag tgagaaacta ttttcttgct tcatttttta
     4681 tgaggatgaa tagaaaatca ttgatcttag caaatagaaa ggcagatgaa acggatgaaa
     4741 ttgcgaacaa atgcaggacc attacaggga acgattcaag ttccaggaga caagtccatt
     4801 agtcaccggg cagtcatatt aggagccgtt gctaaagggg aaacacgagt aaaaggactc
     4861 ttaaaaggag aagacgtgct ttccacgatt caagccttta ggaatttggg ggtccgtatc
     4921 gaagaaaaag atgaccagct tgtgatagaa ggtcaaggtt ttcaaggatt gaccgctcct
     4981 tgtcagacac ttaatatggg aaattctgga acctctatgc gcctcattgc tggattattg
     5041 gcaggtcaac cttttagtgt caaaatgatt ggcgatgaaa gcttatccaa acgtcctatg
     5101 gatcggattg tttatccgtt aaagcaaatg ggggttgaga tatctggaga aacagatagg
     5161 caatttcctc cactacaatt gcaaggtaat cgtaatttac aaccaatcac atataccttg
     5221 cctatctctt ctgctcaggt taagtcagct attttattag cagctttgca agcaaaagga
     5281 acaacacagg ttgttgaaaa agagattact cgaaaccata cagaagaaat gattcaacaa
     5341 tttggtggac gattgatagt tgatggtaaa cgcattactt tggtgggacc ccagcagctc
     5401 actgctcaag agataactgt ccccggagat atttctagtg cagctttttg gcttgtcgcc
     5461 ggcttaatta ttcctggctc ggagctcctt ttaaagaatg ttggagtcaa tccgacacga
     5521 acagggattc ttgaagtggt tgaaaaaatg ggagcacaga ttgtctatga agatatgaat
     5581 aagaaagagc aagtaactag tattcgagtg gtatattctc acttgaaagg aactatcata
     5641 tcaggaggtc ttatccctcg attgattgat gaacttccca tcattgcact tcttgcaacc
     5701 caagcacaag gaacaacctg tattaaagat gctcaagaat tacgagtcaa agaaacagat
     5761 cgtattcaag tagtaactga tactcttaac agtatgggag ctaatatcaa agcgacagca
     5821 gatggcatga ttatcaaagg ccctacggta ctatatgggg ctaacacaag tacctatgga
     5881 gatcatcgta ttggtatgat gacagcaatt gcagccttgc ttgttaaaca aggacaggtc
     5941 catttggata aagaagaagc catcatgacg tcttatccga cgtttttcaa agatttagaa
     6001 aggctgtgtc atgactaaag tgttattggg atttatggga gtggggaaaa caacggtttc
     6061 gaaacaccta agtatgcact gtaaagatat ggatgctatt attgaagcta aaatcggtat
     6121 gtctattgca gctttttttg aacagcatgg agaaatagca tttcgaacaa ttgaaagtca
     6181 agtgttaaaa gatctattgt ttgcaaatga taacagtgtc atcgtaacag gtggcggtgt
     6241 agtggtttta caagaaaatc gacaattact gagaaaaaat caccagcata atattttatt
     6301 ggtagcttct tttgaaactt tgtaccagcg tttaaaacat gataaaaagt ctcaaagacc
     6361 tctatttctt aagtactcta aggaagcatt ttatgaattt tatcaacaac gtatggtatt
     6421 ttatgaggga ctttcagatt tagtaatcag agttgaccat cgtacgcctg aagaagttgc
     6481 caacatcatt gagggttatt agcaggtcat tgtcgtaatc tgatatttat ggttttaaac
     6541 aggtatctgt ctaaagcact tcttatggca agttggcttt attaaatatt aggaaataat
     6601 cttgattatt ctataatgat taaaattttg agaggacaat ctggctaata taggttataa
     6661 tggtagtatt cgttaaaagt gcaattgtaa tactaagaga tgataagata tgacaaaata
     6721 ccccatgggt ggccttagtc accatgaaga attacgttat ttttatctcc taaggaattt
     6781 gagttacctt agtgagaatg aaaaaaaaga gtttgctttt ttaaaatcta aattggaaat
     6841 tggtcgagct tatgcgcctt ctaagcagca ttataggaag tcaaaacggc aagaacctta
     6901 ctttgaagat gactactata acgactactc accaaatgat ttactagaag atgatgacgt
     6961 caatcatgac tcttcttttg ttccatatgg tttaccgata tatcctaaag aagacagata
     7021 tttaaacaaa aaaacaaaac ttacagctcg tagacctata gatgctccgc agcctattga
     7081 tgaagacgat gcttttttaa cagagtctgt tgctcgttgt gctcttccta gatctcaaaa
     7141 aagaaagcat aagaaaaaag gatgtatgaa atggtttttt aacattcttg gcttattgct
     7201 aatgactgtg cttatggggt tagggttgat gtttgcaaaa ggggtttttg atatttctac
     7261 taataaagct aattacaaac cagctgtttc ccaagctttt gatggtcaag aaactcaaga
     7321 tggtacgaac attctggttt tgggttctga ccaacgagtt actcaaggtt ctacagatgc
     7381 tcgcacagat accattatgg ttgtcaatgt cggcaaccat gctaaaaaaa taaaaatggt
     7441 ctcttttatg cgtgacactt tgattaacat tccaggatac agttataatg ataattcgta
     7501 tgatcttaaa ttaaatagtg catttaatct tggagagcaa gaagatcacc acggagcaga
     7561 gtacgttcga cgggccttaa aacataattt tgatatcgac attaagtatt atatgatggt
     7621 agattttgaa acatttgcag aagctattga caccttgttt ccaaatggtg ttaagattga
     7681 tgccaaattt gcgacagtag gtggtgtagc tgttgactcc gtagaagttc cagatgacct
     7741 gcgaatgaaa aatggggttg ttcccaacca aaccatcgaa gttggagaac aacgcatgga
     7801 tggtagaacc ttgctcaact atgctcgttt ccgtaaagat gacgaaggag atttcggacg
     7861 aaccgtccga cagcaacagg ttatgtcagc tgttatgtcc caaatcaagg acccaactaa
     7921 actctttaca gggtctgcgg ctattggtaa aatctatgcg ctgacttcga ccaatgtttc
     7981 ttttccattt gttgttaaaa atggtgtttc tgttttagga agtgggaaaa atggagttga
     8041 gcacgtgacc atcccagaaa atggggattg ggttgacgaa tacgatatgt atggaggaca
     8101 agccctttac atagattttg ataagtatca gaaaacgtta gccaaattag gtctgcgcta
     8161 agatatggta taatggagag taactaaagt tgctcttctt ttttgagcaa tggttagccc
     8221 tagaaaagga aaaagaataa gaatgttgaa aaaaaatgat attatccaag ttgctatttc
     8281 agatttaagc catgagggag caggtgtcgc taaacacgat ggttttgttt tctttgttga
     8341 taatgcattg ccagaagaag tgattgacat gcgtgtctta aaggtcaata aaaatagcgg
     8401 ttttggcaaa gtagaagcct atcattacct gtcgccagct agaaatgcag atgtcaattt
     8461 aacttatttg cgaacaggca ttgcagacct tggacatcta acttatgaag accaactgac
     8521 tttcaaaaag aaacaagttc aggacagcct ctataaaatt gctggaatca gtgacgtgac
     8581 agtcgaaagc acgattggga tgacagaacc tttagcttac cgaaataagg ctcaagttcc
     8641 tgttagacgt gttaacggtc agctagaaac aggctttttc cgcaagcatt ctcatgactt
     8701 gattcctatt tcggattatt atattcagga caaagagatt gatcgtctca ttaattttac
     8761 ccgtgattta ttacgccgct ttgatattaa accgtatgac gaaacagaac aaacaggtct
     8821 cttgagaaat attgttgttc gccgaggcca ttactctgga gaaatgatgc tggtcttagt
     8881 gactacacgc ccaaaagttt tccgtgttga ccaagtcatt gaaaaaatcg ttgaagcttt
     8941 tccagcagtg gtgtctatta ttcaaaatat taatgataag aacaccaatg caatctttgg
     9001 taaagatttc aaaacgcttt acggcaagga taccattact gatagcatgc ttggaaacaa
     9061 ctatgccatt tctgctcagt ctttctacca agtgaatacg attatggcag aaaaactcta
     9121 tcaaacagct atagcctttt cagacctttc taaagatgac attgtgattg acgcctactc
     9181 aggtatcgga accattggcc tatcatttgc taaaacagtt aaagctgttt atggtgttga
     9241 agtgattgaa gcagctgtta gagatgctca acagaatgcc gctcttaatg gcatcaccaa
     9301 tgcctacttt gtcgcagata ccgcagaaca tgccatggca acatgggcga aagatggcat
     9361 taaaccaagt gttattctgg tagacccgcc acgaaaaggc ttaacagaaa gtttcatcca
     9421 agccagcgtg gcaatgggac cacaaaaaat catctacatt tcctgcaacc cagccaccat
     9481 ggcgcgtgac atcaagcgtt atcaagagtt gggttacaaa ctgaccaaag tgcagcccgt
     9541 agatttgttt ccgcaaacgc atcacgtcga gtgtgtagtg ttgctaatca aggagtaagg
     9601 tagctgcctc cacatgacaa aaataaggag aaacgaatgc ctattaccca acacgagatt
     9661 ccccttttag aatgtgatag taatccatca gccgtcgtta tacctacaca tgaaggtcta
     9721 gctatttagc ttccagctaa gtgtgtctat gctttcttag aagacgagat tgaagctttt
     9781 gctttagacc atcaaggtaa aaaaagtagc agaattcatt tcagccacaa aggtttaccc
     9841 tgtttatcag atatcttata aaggtcaaga catttgccta gctcaagctc ccgttggctc
     9901 agctcctgcg gcgcaattca tggactggct gattggttat gaggttaaag aaatgatcag
     9961 tacaggaacc tgtggcgttt tggttcctat cgcagaaaac cgttttttag tgccagttaa
    10021 ggctttgcga gatgaaggaa caagttatca ttacgtggca ccatctcgtt atattgatat
    10081 tgatcctaag atgcttagac tgattgagaa aagcttttag cacaggggtt agcttatcaa
    10141 gaggtcatca cctggtcaac agatggtttt taccgagaaa ccaaagaaaa agtggcttat
    10201 cgccaggaag aagggtgttc ggttgttgag atggaatgtt ctgccttagc agcagtagct
    10261 caattaagag gcatcctttg gggacaactc ttatttacag cagatactct agcagatgta
    10321 gaggtttatg atcaacgcaa ctggggcgct gattccttta gttttgcgct ccatttatgc
    10381 ttggaagtac tcaatacgct agaaaaggat ggaaaggcta ctgatttttg aaacatgagc
    10441 tctgccttct gttcctattt gtagtataat gaggcttgta ttgcttttag actaagctta
    10501 gaaaagagaa aaacaatgtc agatttaaca caagagatga ccttaaatgt tattagtatt
    10561 tttgataatt accaaattga acttgctgaa aaaggacacc ttattttaag tactgaagtg
    10621 actgagacgg ctttgaatta ttatgggaat gcccatggtg gttatctttt caccctatgt
    10681 gatcaagtag gtggtttggt tgctagaact actggcgtag aatctgtcac cttacaggcc
    10741 aatgctaact atttaaaagc agggcacaag ggagacaagc tgatggttga aggtcgattg
    10801 gttcatggag gtcgaaccac gcaggtggtt gatgtgacga ttcataacca aacaggagct
    10861 cttctaacaa aaataagctt gaccatgttt gtgactggcc gacgtcataa cgggtgatta
    10921 atgaatcaat aaaggaaaaa agtcccaact ctctgagaat tgggactttt ttcctttatt
    10981 gagagatacc ataaatcacc cgttagacgg gtggttatgg tttatttacg gtaaagtttg
    11041 tgatattggt aaacagggtc aaaggtaata tttaccccaa gttttcggag tacattttta
    11101 tcttcttcgg ttaagataac ggtagaatgg gcttcgctac ctttcaaatt gccaagttct
    11161 ttcatggcta gattagcttc ttcactagtc atagcagtaa tggccaacgc aatcagaatt
    11221 tcattggaat gaagccgcgg attgtgactg cctaagtggt tcactttgag gccttgaata
    11281 ggtttcacgt attcgggttc aatcaaatga gtggttttat caatctttgc caaggtttta
    11341 atcgcattga tgataacagc tgcagttggt ccaaagagtt cagatgtttt acctgtcaca
    11401 atctgcccat ttggtagttg gagagcaagg gcaggttggc ctgtttgctc tgctttttga
    11461 tgagcagcta cagtaacgtg acgatcgtca ggggtgacac cgatatcatt catcaagagc
    11521 tcaatttttt tgacagcaga ttctgtgact cgctctgcct taaaatccac gagggtttgg
    11581 taataacgac ggatgatttc ttgtttagaa gcttcgatag cagcttcttc attgacgata
    11641 gaaaaaccaa ccatgttaac acccatatcg gttggtgagg cataaggaga cttgctcaaa
    11701 atacgctcga aggtccgatt taagacagga aagacctcga tatcacggtt gtaattaacg
    11761 gccgttttgc cataagtttc caaatggaag gggtcaatca tattcacatc atctaggtct
    11821 gccgtggctg cctcataagc cagattgaca gggtgatgaa gaggaagatt ccaaactggg
    11881 aaggtttcaa atttagcata gccagactta accccattga tttggtcgtg atacatttgg
    11941 gacatgcaag tggctaattt tccagaccca gggcctggtg ctgtaacaac gataaggtta
    12001 cgactcgttt tgatgtaatc atttttcccc atcccctcag atgagataat atgattaata
    12061 tctgtcgggt agcccttgat aggataatgc aggtaagaag caatgccatt tttttcgagt
    12121 tgcttgcgaa aggcatctgc agcgggttgg ttattgtatt gagtaataac gacagaacct
    12181 acgtaaatat ctaacgtgtt aaatttgtca attaagcgaa agacttcttg gtcataagaa
    12241 atgcctaaat caccacgcgc tttagaatgt tcaatattat tagcgttaat ggcaattaca
    12301 atttcgactt gttctttgag ctctttgagt aatttaatct tattgtcagg ttcatagcca
    12361 ggaagcacac gggcagcatg gtaatcttct aacattttac caccaaattc catgtaaagc
    12421 ttgccatcaa attggctgat acgttctaaa atatgatctc tttgcaagtt taaatacttg
    12481 ttagaatcaa aagcaatagt cttcattttt tcacctctgt aaagcattat aacaaagaaa
    12541 ttccaagaaa gaaaaggttt tttgcaggga tacctcattt tgtgactttt taaaaaaact
    12601 taggcttggt tcttagaaga aaaatgtatc cgctatgttc aaaagaactt gacaattaaa
    12661 atgagcttta ctatactagt aacaattaaa tactatttta aacatgttcc gtgaagcatg
    12721 aaagtcttaa cagtaaagag agtttttttg tcatctctta ttatttagtg aactttgatg
    12781 gacttgcgga cttgtttcca agtctttttt tgacctttgg acaaagggta ttcaattaat
    12841 taaggtagtc agtgagctac tattaagaag gagacaatta tgtcaacatt atcattggac
    12901 accacaaata agcgagcttt agtggcagct attgcagctt caggaacgga tgacttgaat
    12961 gtcatgtttc ttgccttttc catgtcctcc attatgacag acttggggct tagtggcact
    13021 cagggtggct ggattgcgac cattaccaat ttaggaatgt tggtgggagg cctcttattc
    13081 gggttgctgg ctgataggca ccacaagttt aaggtcttta aatggacgat tttactgttt
    13141 tcagtagcaa caggattaat ttattttacc cagtctttgc cctacttgta cttgatgcga
    13201 tttattgctg gcataggagt tggaggtgag tacggtatag ccattgctat aatggcaggg
    13261 attgtaccac cagaaaaaat gggtcggatg tcttctttaa atggtattgc tggccagctt
    13321 ggctccatta gttcggctct tttagcaggg tggctggctc taagtctagg ctggcgtggc
    13381 ctttttctct ttggattact tccgattctc ttggtgattt ggatgacttt agccatagat
    13441 gaccaaaaaa tttgggatca ttatgggcaa gaggaggaag aatgcagtca accaattaaa
    13501 atcaatgaac tattcaaaac aaaatcctta acagctcaaa cccttgcttt aatggtcatg
    13561 acgactgtgc agattgcggg ctattttgga atgatgaatt ggttgccaac cattattcaa
    13621 acaagcttaa acctttcagt aaaaagttcg tccttgtgga tggtggctac tattgtcggc
    13681 atgtgtttgg ggatgctgtg ttttggtcag cttttagatt gctttggtcc acgtctgatt
    13741 tattccctct ttttattagc atcatctatt tgtgtttacc tttttcaatt tgccaattcc
    13801 atggcgagta tggttattgg aggtgctatt gtcggttttt ttgtcaatgg gatgtttgct
    13861 ggctacgggg ctatgattac cagactttat ccccatcaca ttcgatccac ggctaataat
    13921 gttattttga atgttggccg tgctctagga ggattttcgt ccgttgctat tggtagtatt
    13981 ttagatgcct caggtatttc aatggtcatg attttcctag cttctctgta tgtgattagt
    14041 tttggagcca tgtggactat tggtcagtta aaagcagaac gctaccaaca attgcgttag
    14101 caaagagatg ggtgttagta tttgagttac tttcactcac aaaactattt tattgattga
    14161 gataagcaaa aaggtaagcg cttagggatt gcttaccttt ttggttcttt tcttttgttt
    14221 tactccgcta ctttggccat aaaatgttta ctgttcaccc taactctagt atgacaggct
    14281 tttccaatga gttgttctcc cacaaaagct tccaagcgga aatcgtattt tcggccttct
    14341 tctttaaggg ctgtaatgat gattgtaaca ggatctccaa tggctgaagc tgccaaatgt
    14401 tggatagcta tctcgctgcc aaccgttgta aagtcactgt cgagctgttc ttgggcaaat
    14461 aagtaagaag cattttccat aaaagcaaca agagcaggag tagctaggac attcagcgtt
    14521 cctgagccca tggctttagc cgaatgattg ggacgggttt cataagtgtg agaataaata
    14581 gtcataagtt aatctcctcg tttctataag cctattataa tatattaagc gcttacagtc
    14641 atgaagccac tgttttttat gttatactta ataatgaatt ttctttgaaa gggttatgat
    14701 gcgcttagat aaatttttag tggcaactgg tgttggaact aggagccaag tcaaacttct
    14761 cttaaaaaag aaggctattt tcgtcaatca aaaggttgaa acctctgcca aagcacacat
    14821 tgatgaatac aaggatttag tgacgtatca aggcacgccc ttggtctatg aatcctttgt
    14881 ttattacctg ttaaacaagc cgtcagggta tgtgtcagca acccaggaca gacagcaggc
    14941 tactgttatg gagttgttag atgacacggc taggcaaaaa gcagtttttc ctgtgggtag
    15001 gttagataag gatacccgtg gacttttgct cttgaccaat aatggacaac tagcccatga
    15061 ccttttatcc cctaaaaaac atgttacgaa agagtacttg gctaaagtag caggcatcat
    15121 gactgaagct gataaagatt attttgctag aggtatttcc ttaaaggatc accagtgcct
    15181 acctgctcat ttagaagtgc ttgctagtga tttacagcag caaacaagtt tggttaaaat
    15241 taccatacaa gaaggaaaat tccatcaggt gaaacgcatg gtagcagcct gtggaaagga
    15301 agtgcttgac ttacagcgtc tcagcatggg accattaaaa ctagacccta gccttgcaga
    15361 aggggagttt aggcgcctga cgcctgagga attgcaaagc ctggcacctt attgtcagag
    15421 ctcactataa aaagaaacct cccctgtcag tactgacccc aaaaagttgg acaaataatt
    15481 attgaaagga tttagttctg tattggacag gactaagtcc ttttagtttt gcttt
//
LOCUS       DAUDYX010000048        14669 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.049, whole genome shotgun sequence.
ACCESSION   DAUDYX010000048 DAUDYX010000000
VERSION     DAUDYX010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14669
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.049"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            27..731
                     /locus_tag="VHD02_001517"
     CDS             27..731
                     /locus_tag="VHD02_001517"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEQ5498114.1"
                     /translation="MTNQLIYKGKAKDIYSTKDENVIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNKAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVIAGSFSK
                     RFGVEEGRVLETPIVEFYYKNDDLNDPFINDEHVKFLGIVNDEEIAYLKGETRRINEL
                     LKGWFAQIGLNLIDFKLEFGFDQEGTIILADEFSPDNCRLWDKNGNHMDKDVFRRDLG
                     NLTDVYQVVLEKLIAL"
     gene            855..4485
                     /locus_tag="VHD02_001518"
                     /pseudo
     CDS             855..4485
                     /locus_tag="VHD02_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
     gene            4646..6100
                     /locus_tag="VHD02_001519"
     CDS             4646..6100
                     /locus_tag="VHD02_001519"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEQ5498115.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNSSFMGKVKE
                     ALNTVKGGFAYLLMTENKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDRGIQCDRYTDETQLAICSMEYVYFARPDSTIHGVNVHTVRKNMGK
                     RLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAADEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEKTSFYIQKVK"
     gene            6128..7150
                     /locus_tag="VHD02_001520"
     CDS             6128..7150
                     /locus_tag="VHD02_001520"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEQ5498116.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVREPVLVSGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNNPVKFEEVVSGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQLIDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGKELLPELEGKQLK
                     DVLLEPTRIYVRAALPLIKEELVNGIGHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     DSVVIK"
     gene            7318..7872
                     /gene="purN"
                     /locus_tag="VHD02_001521"
     CDS             7318..7872
                     /gene="purN"
                     /locus_tag="VHD02_001521"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEQ5498117.1"
                     /translation="MKIAVFASGNGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKAAYEQAIVDLLDKHEIDLVCLTGYMKIVGETLLLAYEGRIINIHP
                     AYLPEFPGAHGIEDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPAVLDSLGVERKVI"
     gene            8056..9603
                     /gene="purH"
                     /locus_tag="VHD02_001522"
     CDS             8056..9603
                     /gene="purH"
                     /locus_tag="VHD02_001522"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEQ5498118.1"
                     /translation="MTKRALISVSDKSGIVDFAKELKNLGWDIISTGGTKVALDNAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDITYDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADA
                     GQTTFETRQRLAAKVFRHTAAYDALIAEYFTTQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVVLKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAKKMHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEATALEFAWKAIKYVKSNGIIITNDHMTLGLGAGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQDSIDAANKHGLTMIFT
                     GVRHFRH"
     gene            complement(9661..10785)
                     /locus_tag="VHD02_001523"
     CDS             complement(9661..10785)
                     /locus_tag="VHD02_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEQ5498119.1"
                     /translation="MKKFHRFLVSGVILLGFNGLVPTMPSTLISQQENLVHAAVLGDN
                     YPSKWKKGNGIDSWNMYIRQCTSFAAFRLSSANGFQLPKGYGNACTWGHIAKNQGYPV
                     NKTPSIGAIAWFDKNAYQSNAAYGHVAWVADIRGDTVTIEEYNYNAGQGPERYHKRQI
                     PKSQVSGYIHFKDLSSQTSHSYPRQLKHISQASFDPSGTYHFTTRLPVKGQTSIDSPD
                     LAYYEAGQSVYYDKVVTAGGYTWLSYLSFSGNRRYIPIKEPAQSVVQNDNTKPSIKVG
                     DTVTFPGVFRVDQLVNNLIVNKELAGGDPTPLNWIDPTPLDETDNQGKVLGDQILRVG
                     EYFTVTGSYKVLKIDQPSNGIYVQIGSRGTWVNADKANKL"
     gene            11038..12303
                     /gene="purD"
                     /locus_tag="VHD02_001524"
     CDS             11038..12303
                     /gene="purD"
                     /locus_tag="VHD02_001524"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEQ5498120.1"
                     /translation="MKLLVVGSGGREHAIAKKLLASKGVDQVFVAPGNDGMTLDGLDL
                     VNIVVSEHSRLIAFAKENEISWAFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTFSDFEKAKAYIEEQGAPIVVKADGLAVGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFVNGDKFYIMPTAQDHKRAF
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVETIVKPVLEGMIAEGRPYLGVLYAGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKTDGDIITYYAGVKFAENSELLLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDPTGLFYRNDIGSKAIRE"
     gene            12581..13072
                     /gene="purE"
                     /locus_tag="VHD02_001525"
     CDS             12581..13072
                     /gene="purE"
                     /locus_tag="VHD02_001525"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001875905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEQ5498121.1"
                     /translation="MKTPIISIIMGSKSDWATMQKTAEVLDNFGIAYEKKVVSAHRTP
                     DLMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDSLYS
                     IVQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSG
                     ELI"
     gene            13059..14132
                     /gene="purK"
                     /locus_tag="VHD02_001526"
     CDS             13059..14132
                     /gene="purK"
                     /locus_tag="VHD02_001526"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEQ5498122.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGHKVITLDPANDCPAARV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENVDADGLDAVVSAGQLPQGTDLLRISQNR
                     IVEKDFLTNKAGVTVAPYKVVTSSLDLEGLDLTKTYVLKTATGGYDGHGQKVIRSAED
                     LPEAQQLANSAQCVLEEFVNFDLEISVIVSGNGQDVTVFPVQENIHRNNILSKTIVPA
                     RISDQLADKAKKMAVQIAKKLQLSGTLCVEMFATADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPTIHLHAPAVMLNVLGQHVQQAIDYVTENPSAHLHMYVKLE
                     AKHNRKMGHVTVFAEDADEVEEF"
     gene            14153..>14669
                     /locus_tag="VHD02_001527"
     CDS             14153..>14669
                     /locus_tag="VHD02_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498123.1"
                     /translation="MELREFKAWLLKNHFEEDELYKESIKCYQVEAYKAAYIFSYLAN
                     HKFIAKLAIDYSGVPSNFMSKCPDDEVRKSSWQKRVNSLKNEDEWEQTVNEKLIQAWE
                     GDNVFKLPFKIKEKYSHMRVLRNNAAHAKDCRISESTVLELWNEIEYIYPYFVINGTI
                     DAWMEDLEKLVR"
BASE COUNT         4194 a         2877 c         3459 g         4139 t
ORIGIN      
        1 aaacagaaag aaaggcgaat aatcaagtga ctaaccagct tatttataaa ggaaaagcga
       61 aagatattta tagtacaaaa gatgaaaatg tgattagaac ggtctataaa gaccaagcta
      121 ctatgttgaa tggagctcgt aaggagacta ttgatggcaa gggagcacta aataatcaga
      181 tttcatctct tatttttgag aaattaaata aggctggtgt ggtgacacat tatattgagc
      241 aaatttcaaa aaacgaacaa ttgaataaaa aagttgatat tattccactt gaggtggtgt
      301 tacggaatgt gatagcaggc tcattttcaa aacgttttgg agtggaagaa ggacgtgttc
      361 ttgaaacgcc aattgtggaa ttttactaca aaaatgatga tctgaatgat ccttttatca
      421 atgatgagca tgtgaagttt ttgggtattg ttaatgatga ggagattgct tatctgaaag
      481 gagagactcg ccgtattaat gaactcttga aaggttggtt tgctcagatt gggcttaact
      541 tgattgactt caagttggag ttcggatttg atcaagaagg tacaattatc ttagcagatg
      601 aattttcgcc tgataactgt cgcctatggg ataagaatgg taaccatatg gacaaggatg
      661 tttttagaag agaccttggc aacctgacgg atgtctatca agttgtactt gagaagttga
      721 tagcattgta actagaagag gaaggaattg aatccgttcg ggaaactccg aaaaaaagat
      781 ggaacttact gaaatctcaa tttcagatgt cctcctattt tccagtcgct cccttgtcgg
      841 acttggtatc ttatatgaat aaacgtattt ttgttgagaa aaaggctgac tttggtatta
      901 aatcggctag ccttgtgaaa gagttgacgc ataatctaca actgacttct ttgaaggctt
      961 tgcgtattgt gcaggtctat gatgtcttca atttggctga ggatttgctg gcgcgtgctg
     1021 agaagcatat tttctctgag caggtgacag actgtctttt gacggaaact gaaatcactg
     1081 cggagcttga taaggttgcc ttctttgcca ttgagtcgct tcctggtcaa taaggatatt
     1141 gcagaagcag agcttgaagc cgttaagaac tatcttttga accctgttga ttcgcgtttc
     1201 aaggacatta ctttgccgct tgaagagcag gctttctctg tgtctgataa gacgatccct
     1261 aatcttgatt tcttcgagac ttataaggct gacgatttta cggcttataa ggtagagcag
     1321 ggcttggcta tggaggtcga tgatcttctc ttcatccaag attatttcaa atcaatcggg
     1381 cgtgtgccaa ctgagactga gttgaaagtt ttggatacgt attggtcaga ccactgtcgt
     1441 cacacaacct ttgaaactga attgaagaac attgattttt cagcttctaa attccaaaaa
     1501 caattgcaga caacttatga caaatatatc gccatgcgtg atgagcttgg tcgttctgaa
     1561 aagccacaaa cacttatgga tatggcgact atttttggtc gttatgagcg tgccaacggt
     1621 cgtctggacg atatggaagt ctcagatgaa atcaatgcct gctcagttga gattgaagta
     1681 gatgttgatg gtgtgaaaga gccttggctc ctcatgttta agaatgagac acacaatcac
     1741 ccaacagaaa ttgagccatt cggtggagcg gcgacttgta tcggtggtgc tattcgtgac
     1801 ccattgtcag gacgttcata cgtttatcag gctatgcgta tttcaggcgc aggcgatatc
     1861 acgactccga ttgcggaaac acgtgctggt aaattgccac aacaggttat ttctaagact
     1921 gcggcgcacg gctattcttc atatggtaac caaattggac ttgcgacaac ttatgtgcgt
     1981 gagtacttcc accctggctt cgtagccaaa cgtatggagc ttggagctgt ggttggtgct
     2041 gcacctaagg aaaatgtggt tcgtgaaaaa ccagaagcag gtgatgtggt aatcttgctt
     2101 ggtggtaaaa cagggcgtga cggtgtcggc ggtgcgacag gttcatctaa ggttcaaacg
     2161 gttgaatctg tggaaacagc aggagcagag gtacaaaaag ggaatgccat cgaagagcgt
     2221 aagattcaac gtcttttccg tgctggcaat gtcactcgtc tgatcaagaa atcaaatgac
     2281 ttcggtgcag gtggtgtctg tgttgccatc ggtgaattgg ctgacggtct tgaaatcgat
     2341 ttggacaagg tgcctcttaa ataccaaggt cttaatggta ctgaaattgc aatctcagaa
     2401 tctcaagagc gtatgtcagt cgttgttcgt ccaagtgatg tggatgcctt catcgcagcc
     2461 tgcaacaagg aaaatatcga tgcagtcgta gttgcgactg tgactgaaaa accaaatctt
     2521 gtcatgactt ggaatgatga aacaattgtt gatttggaac gccgtttcct tgataccaat
     2581 ggtgtccgtg tcgttgttga tgctaaagtc gttgacaagg acttgacagt tccagaagca
     2641 cgcataacat cagcagagac acttgaagca gatacgctta aggtcttgtc tgacctcaac
     2701 catgctagtc aaaaaggtct ccaaaccatc tttgactcat ctgttggtcg ttcaaccgtt
     2761 aaccacccaa tcggtggtcg ttaccaaatc acaccgacag aaagttctgt tcaaaaattg
     2821 ccagttcaac atggtgtgac aacaactgca tctgttatgg ctcaaggtta caatgcttat
     2881 attgcagagt ggtcacctta tcacggtgct gcctatgctg ttattgaagc gacagctcgc
     2941 ttggtagcaa caggtgctga ctggtctcgt gcacgtttct cttaccaaga gtatttcgag
     3001 cgtatggata aacaggcaga gcgttttggt cagccagtat cagctcttct tggttctatt
     3061 gaggctcaga ttcaacttgg cttgccatca atcggtggta aggactctat gtctggtact
     3121 ttcgaagact tgacagtacc accaaccttg gtagctttcg gtgtgacaac agcggacagc
     3181 cgcaagattc tctctcctga gtttaaagcg gctggtgaga atatctacta cattccaggt
     3241 caagctattt cagaagatat tgattttgac cttatcaagg ctaacttcag tcagtttgaa
     3301 gctattcaaa ctcaacataa gattacagct gcctcagccg ttaaatacgg tggtgtccta
     3361 gaaagttttg ctctcatgac ttttggtaac cgtatcggtg cttctgttga aattgcagag
     3421 cttgacagca gcttgacagc tcaactcgga ggttttgtct ttacatcagc tgaggaaatt
     3481 gctgacgcgg tgaaaatcgg tcaaactcag gcagacttta cagtcactgt caatggaaat
     3541 gaccttgctg gcgctagcct tctagcagcc ttcgaaggca aattggaaga ggtttaccca
     3601 acagaatttg aacagacaga tgttcttgaa gaagttcctg ctgtggtatc agatactgtt
     3661 atcaaggcta aggaaacaat tgaaaaacca gtggtttaca ttccagtctt ccctggtacc
     3721 aactcagagt acgattcagc taaggccttt gaacaggttg gagctagtgt caacttggta
     3781 ccatttgtaa ccttgaatga ggttgctatt gctgagtcag ttgacactat ggttgctaat
     3841 attgctaagg caaatatcat cttctttgct ggaggtttct cagcagcgga tgaaccagat
     3901 gggtctgcta agtttatcgt caatatcttg cttaacgaga aggtccgcgc agctattgac
     3961 agcttcatcg aaaaaggtgg ccttatcatc ggtatctgta atggtttcca agcccttgtt
     4021 aaatcaggtc ttcttccata cggaaacttc gaggaagctg gtgagacaag tccaactctc
     4081 ttctataacg atgctaatca gcatgttgcc aagatggttg agactcgtat cgcaaatacc
     4141 aactcacctt ggttggcagg agttgaggtc ggcgatattc atgccattcc agtttcacat
     4201 ggtgaaggta aatttgttgt cagcgcttct gaatttgcag agctaagaga caatggtcaa
     4261 atctggagcc aatatgtgga ctttgacgga caaccatcta tggattctaa atacaatcca
     4321 aacggctctg tcaatgccat cgaagggatt accagcaaga atggtcaaat catcggtaag
     4381 atgggacact cagaacgctg ggaagacgga ctcttccaaa atattcctgg taacaaagac
     4441 caaaccctct ttgcaagtgc tgtaaaatac tttacaggga agtaaaaagg ttcagtgaac
     4501 ctttttagcc ctcgccttga attagtgaag ggcgaggtta gaaagaatct cgcttaattt
     4561 ttgagaaata gtaaacacgg gcgataagtt gtgcgaaaaa ctttctattt tcgctttact
     4621 tgacagcctt tgtatcgagg taaaaatgac atacgaagta aaatctctaa atgaagaatg
     4681 tggagtcttt ggtatctggg gacatcctca ggcagctcaa gtcacttact ttgggcttca
     4741 tagccttcaa catcgcggtc aagaaggagc tggtattgtt tcaaatgaca atgggaaact
     4801 ctatggttat cgaaatgttg ggctcctttc tgaagttttt aagaatcaat ctgaattaga
     4861 taatttaact gggaatgcgg ctattggaca tgttcggtat gctactgcag gttctgcaga
     4921 tattcgcaat attcagcctt ttctttataa atttcatgac gggcaatttg ctttatgcca
     4981 taatggtaat ttgacaaatg ctatttcctt aaggaaagaa ttagaaaagc aaggtgcaat
     5041 tttcaatgcc tcctcagata ctgaaatttt gatgcacttg attcgtcgaa gccataactc
     5101 aagtttcatg ggaaaggtaa aagaagctct aaatacagta aagggtggct ttgcctatct
     5161 actgatgaca gaaaataaac tcattgctgc tcttgaccct aatgcctttc gtcctttgtc
     5221 aattggacaa atgcaaaatg gtgcctgggt tatttccagt gagacctgtg cttttgaggt
     5281 ggtaggcgca aaatgggtta gagatgttga acctggcgaa gttattctta tagacgatcg
     5341 tggtattcaa tgtgatcgtt atactgatga aacgcaactg gcaatttgtt cgatggaata
     5401 tgtctatttt gcaaggccgg attcaactat acatggtgtt aatgttcata cggttcgaaa
     5461 aaatatggga aagcgtcttg cacaagaatt taaacaggat gctgatattg taattggtgt
     5521 cccaaattcg tccttatcgg ctgctatggg ctttgctgaa gaatccggat taccaaatga
     5581 gatgggtctt gtaaaaaatc agtatacgca gcgaaccttt attcaaccga cacaagaatt
     5641 aagggaacaa ggtgttcgaa tgaaactatc agcggtatct ggtgttgtca aaggaaagcg
     5701 cgttgttatg attgatgact caattgtaag aggaacgact tctagaagga ttgtcggatt
     5761 attaagagaa gcaggagcta gtgaagtaca tgttgctata gctagtccag aattaaagta
     5821 tccttgtttt tatggtattg atattcaaac aagacgagaa ttaatttcag caaatcacgc
     5881 tgctgacgaa gtgtgtgata ttattggcgc agatagtctg acctatctct cactagatgg
     5941 gttgattgaa tcaattggac tcgaaacaaa agcaccaaat ggtggtttat gcgtagctta
     6001 ctttgatgga cattatccaa caccgcttta tgattatgaa gaagaatacc tcagaagttt
     6061 agaagaaaaa acaagttttt atattcaaaa agtgaaataa gctctgctaa ttatataagg
     6121 agaaaacatg tctgaaaaaa atgcttatgc aaaatctggt gttgatgttg aagctggtta
     6181 tgaagtggtt gaaagaataa aaaaacacgt tgctcgtaca gaacgtgcgg gagtcatggg
     6241 agctctaggt ggctttggtg ggatgtttga cttgagtaaa acaggagtta gagagcctgt
     6301 tttggtctca gggacagacg gtgttggcac aaaactcatg cttgctatca agtacgacaa
     6361 gcacgacaca atcggtcaag actgtgttgc catgtgtgtc aatgatatta ttgcagcagg
     6421 tgctgagccc ctttacttcc ttgactacgt agcgacagga aaaaataatc cagtcaaatt
     6481 tgaagaagtt gtttctggcg ttgcagaagg ttgtgttcaa gcaggagcag cactcattgg
     6541 aggtgagacc gctgaaatgc ctgggatgta tggcgaagat gattatgacc ttgctggctt
     6601 tgctgttggt gtggctgaaa aatctcagct catcgacggt tcaaaagtaa aggaaggcga
     6661 tattcttttg ggacttgctt caagtggtat ccattcaaac ggttattctt tggtacgtcg
     6721 tgtctttgct gactatactg gtaaagagct gcttccagaa cttgaaggaa aacaacttaa
     6781 ggatgtcctt cttgagccaa ctcgtattta tgttagagca gctctgccat taatcaagga
     6841 agaactagtc aacggtatcg gccacatcac aggtggtggc tttatcgaga atgttcctcg
     6901 tatgtttgca gatgatttgg ctgcggaaat cgatgaggac aaggtgccag tgcttccgat
     6961 tttcaaggcg cttgaaaaat atggtgacat caagcacgaa gaaatgtttg aaatcttcaa
     7021 tatgggtgtc ggtcttatgc tagctgttag tcctgaaaat gttaaccgcg tcaaagagct
     7081 cttggatgaa ccagtttatg aaatcggtcg tatcatcaag aaagcagacg atagtgtggt
     7141 gattaaataa gtcactaaat tttataagaa aggagtgttt agttagtttg cctaactgaa
     7201 cacgggatta aatttctagg aaaaaagata aaatgactta gacgcaagcg tcgttgttcg
     7261 ttttcctatt tttctacgaa atttttcggc aagccgaatc atccctttgt atcgaatatg
     7321 aaaatcgctg tttttgcttc tggtaatggt tccaactttc aggtcatagc agagcagttt
     7381 ccagttagtt ttgtcttttc agatcatcgt gatgcctatg ttttagagcg tgctcagaat
     7441 ttagccattc caagcttcgc ttttgaactc aaggagtttg agaataaggc ggcttacgag
     7501 caagccatcg ttgatttgtt ggacaaacac gagattgatt tggtctgtct aacgggctat
     7561 atgaagattg ttggagaaac cttactcttg gcctatgagg ggcgtattat caatattcac
     7621 ccagcctacc tgcctgaatt tccaggtgcc cacggtatcg aggatgcttg ggaagctggt
     7681 gttgaccagt ctggcgtgac catccactgg gttgactctg gtgtggatac cggtcaggtc
     7741 atccaacaag tgcgcgtgcc acgcctagca gacgatagcc tagaaagctt tgaaacccgt
     7801 atccatgaaa ccgaatatca actctaccca gctgtcttag atagcttggg agtggagagg
     7861 aaagtaatct agcctttatc ttaaaatttt aggtaaagtt aggaaagata gtaaagaaga
     7921 attaacgttc caaaaagaaa ggaattgaac ccgtctggaa ggcacagaaa aaagataaac
     7981 atctcctagg cgcaagcgtc ttcgtcgagt ttcctatttt tccttggcct tctagtcgga
     8041 cttggtatct taaatatgac taaacgtgct ttaatctcag tgtctgacaa gtcaggaatt
     8101 gttgactttg caaaagaatt gaaaaacttg ggttgggata ttatctcaac tggtgggact
     8161 aaggttgccc ttgataatgc tggcgttgag accattgcca tcgacgatgt gactggattc
     8221 ccagagatga tggacggtcg tgttaagacc ctccacccaa acattcacgg tgggcttttg
     8281 gctcgtcgcg acgctgacag ccaccttcag gctgctaagg acaacaatat cgagctgatt
     8341 gacctcgtgg ttgtcaacct ctatcccttc aaggagacta tccttcgtcc agatataacc
     8401 tatgatttgg cggtggaaaa catcgacatc ggcggtccat caatgcttcg ctcagccgct
     8461 aaaaatcacg ctagcgtgac cgttgtggtt gacccagcgg attatgcgac tgttttggga
     8521 gaattggctg acgctggtca gacgacattt gaaacgcgtc aacgcttggc agctaaggtc
     8581 tttcgtcaca cagcagccta cgacgctttg attgctgagt acttcacaac tcaagtggga
     8641 gaggctaagc ctgaaaaatt aaccatcact tacgacctta aacaggctat gcgttacgga
     8701 gaaaatccac aacaagacgc tgatttctat caaaaagcct tgccaacaga ctactcaatt
     8761 gcttctgcta aacagctcaa cggaaaagaa ttgtccttca ataatatccg tgatgccgat
     8821 gcagcaatcc gtattatccg tgatttcaaa gatcgtccaa ctgttgttgt cctcaaacac
     8881 atgaacccat gtggtatcgg acaggctgat gatattgaga cagcttggga ttacgcttat
     8941 gaagctgacc cagtttcaat ctttggtgga attgtcgtcc ttaaccgtga agttgacgca
     9001 gcgacagcta agaagatgca ccctatcttc ttggaaatca tcatcgcacc atcatactca
     9061 gaagaagcgc tagctattct cacaaataaa aagaaaaact tgcgaatcct tgagttgccg
     9121 tttgatgccc aagctgccag cgaagtggaa gctgagtaca ctggcgtagt tggtggactt
     9181 ttggtgcaaa accaagacgt tgtggctgaa aatccatctg actggcaagt ggtgacagac
     9241 cgccaaccaa cagaacaaga ggcgactgcc cttgagtttg cctggaaggc tatcaagtat
     9301 gttaagtcta acgggattat tattactaac gatcacatga cgcttggact cggtgcaggt
     9361 caaaccaacc gtgtcggctc agtcaagatt gctatcgagc aggctaaaga ccaccttgac
     9421 ggtgccgttc tagcatcaga tgccttcttc ccatttgcgg acaacattga agaaatcgct
     9481 gccgcaggga tcaaagcaat catccaacca ggtggttcag ttcgtgacca agactctatt
     9541 gacgccgcaa acaaacatgg cttgaccatg atcttcacag gtgtgagaca ttttaggcat
     9601 taataaaaaa atgtggtcta attaaaacca cattttttat taaaagaaaa tataagttaa
     9661 ctataatttg ttagctttat cagcatttac ccatgttcca cgagatccga tttgaacata
     9721 aataccatta cttggttgat caatttttaa tactttataa ctaccagtga cggtaaaata
     9781 ttcacccaca cggagaattt gatctcctaa aacttttcct tggttatctg tttcatctaa
     9841 tggtgtggga tcaatccagt ttagtggagt tgggtctcct ccggctaatt ctttattaac
     9901 gatcaaatta ttaacaagct gatctacacg aaaaacgcca gggaaggtaa cagtatcacc
     9961 gaccttaatg gaaggttttg tattgtcatt ttgaaccaca gactgtgcgg gctctttaat
    10021 gggaatatag cgtcggtttc cagaaaaact gaggtagcta agccatgtat aacctccagc
    10081 agtcacgact ttatcgtaat aaacagattg acctgcttca tagtaagcaa gatcagggct
    10141 atcgatactg gtttgtcctt tgactggtaa tctggttgta aagtgataag ttccagaggg
    10201 gtcaaatgaa gcttgagaaa tgtgttttag ttgtcttggg taggaatgac ttgtctgaga
    10261 tgataagtct ttaaaatgga tataaccact tacctgagat tttggaattt gacgcttatg
    10321 gtatctttca gggccttgtc cagcgttgta attatactct tcgatagtga cagtgtctcc
    10381 acggatatca gctacccatg ctacatgacc gtaagcagca tttgactgat aagcgttttt
    10441 atcaaaccaa gcgatagccc ctatgcttgg tgtcttattc acaggataac cctgattttt
    10501 cgcgatatgt ccccacgtgc aggcattacc gtagccttta ggtaactgaa aaccattagc
    10561 agagcttaaa cgaaaagctg caaaagaagt gcattggcgg atatacatgt tccacgaatc
    10621 gattccattg ccttttttcc acttactcgg atagttatcg cctaaaacag ctgcatgaac
    10681 aagattttcc tgttgcgaaa taagtgtaga tggcatagta ggtactagac cattaaaacc
    10741 taaaaggatt actcctgaga ccaaaaaacg atgaaatttt ttcattaatt acctcctgta
    10801 acttattatt cgaaagcaag aaacaaaaaa tgttattttt agatgattat tttgaaaatt
    10861 ggctttactt gatattaaat aactgtttga ttggctaata gagtgttcct tatttcaaga
    10921 attgtggtct actatcacat ttttacaatg tttcaattat tgataactaa tacattatgt
    10981 aaaaaagcga acgtttatga tataatatct aattataata cgaaagaggt ggatgttttg
    11041 aaattacttg ttgttggttc aggtggtcgt gaacatgcga ttgctaagaa gttgttagcg
    11101 tctaagggtg tggatcaggt ttttgtggca cctggtaatg atggtatgac cttggatggt
    11161 ctggacttgg tgaatatagt agtttccgaa cattccaggt tgattgcctt tgctaaggag
    11221 aatgagattt cttgggcctt tattggtcct gatgatgcgc tagcagctgg tatcgttgat
    11281 gatttcaaca gtgctggact cagagctttc ggtccaacaa aggcagcagc ggagctagag
    11341 tggtcaaaag actttgccaa ggaaatcatg gtcaaataca atgtcccaac agcagcctat
    11401 ggcacatttt cagactttga aaaagccaaa gcctacatcg aagagcaggg cgcacctatc
    11461 gtggtcaagg ctgatggctt ggcggtaggt aagggtgtgg tcgtggctga aaccgttgag
    11521 caggcggtag aggcggcgca agagatgctt ctggacaata agtttggcga ttcaggagcg
    11581 cgtgtggtta tcgaggaatt cttggatggc gaggagtttt ccctctttgc ctttgttaat
    11641 ggtgataagt tctacatcat gccgacagct caggatcaca agcgtgcctt tgacggagac
    11701 aaggggccaa acactggtgg tatgggtgcc tatgcgccag ttcctcatct acctcagagc
    11761 gtggtagaca cagcggttga gacgatcgtt aaacctgtcc ttgagggtat gattgccgaa
    11821 gggcgtcctt atctaggcgt cctctatgcg ggacttatcc tgacggctga tggtcctaag
    11881 gttatcgagt tcaactcacg tttcggtgac cctgaaactc agattatcct ccctcgcctg
    11941 acttccgatt tcgctcagaa tattgacgat atcatgatgg gcattgagcc ttacatcact
    12001 tggcagaagg acggtgtgac tttgggtgtt gtcgttgcct cagaaggcta tccactcgat
    12061 tatgagaaag gtgtgccact gcctgaaaag accgacggtg atatcatcac ctactacgca
    12121 ggagttaagt ttgcagaaaa tagcgagcta cttctctcaa acggaggacg tgtctatatg
    12181 ctcgtcacca cagaagacag cgtcaaagca gggcaggaca aaatctatac ccaactggcc
    12241 caacaagacc caacaggtct cttctaccga aatgacatcg gaagcaaagc tataagagaa
    12301 taagactaac cattcaaata ttagcgtcag caatcaacga ccgaaggtcg ctccaaaaag
    12361 aaagaagaaa ggaactgaac acgggactaa tttcctagga aaaaaataaa actacctgga
    12421 cgcaagcatc atcgttagtt ttctgcgtcg taggcgtctg atgctaaagc acctagtcgc
    12481 cctgacgtag gttcgtcttc gaaatcaaag atttcggact taccgcatat ttttctacgg
    12541 aaagtttcga caagtcgaaa cgcccctttg tatcttaatt atgaaaactc caattatttc
    12601 tattatcatg ggctccaaat ctgactgggc aaccatgcaa aaaacggctg aagttctaga
    12661 caactttggc attgcttacg agaagaaagt cgtctctgct caccgcacgc ccgacctcat
    12721 gttcaagcat gctgaggaag cacgtggtcg tggcatcaaa atcatcatcg caggagcagg
    12781 tggggcagct cacttgcctg gtatggtggc agctaaaaca acgctgcctg ttatcggtgt
    12841 gcctgtcaaa tcacgtgccc tttcaggttt ggattcactc tattcgattg tgcagatgcc
    12901 tggcggtgtg cctgtggcaa ccatggctat cggagaagcg ggagcgacta acgctgccct
    12961 gacagccctc cgtattctct ccatcgagga tcaaaatctg gctgatgcgc tagctcattt
    13021 ccatgaggaa caaggaaaaa tcgcagagga gtcaagtggt gaactcattt aagaccattg
    13081 ggattatcgg tggtggtcag ctggggcaga tgatggcgat tgcggctatc tacatgggcc
    13141 ataaggtcat cacgctggat cctgctaacg actgtcctgc cgcccgcgtt agcgaggtga
    13201 ttgtggcacc ttacgatgat gttgaggctt tgggacaatt agctgcgcgt tgcgatgttt
    13261 tgacctatga gtttgagaat gtcgatgccg atggtctgga tgccgttgtg tcagctggtc
    13321 agctaccgca ggggactgat ctgctccgca tttcccaaaa ccgtatcgtt gaaaaagact
    13381 ttctgacaaa taaggctggt gtgactgtcg ctccttataa ggtggtaacg tctagccttg
    13441 acctagaggg gcttgatttg accaagacct atgtcctcaa gacagcgaca ggtggttatg
    13501 acggtcatgg gcaaaaggtt attcgctccg cagaagacct accagaggcg caacaattag
    13561 ccaactcagc tcagtgtgtc ttggaagagt ttgtcaattt tgaccttgaa atctcagtta
    13621 tcgtgtctgg aaatggtcag gatgtgacgg tctttcccgt tcaggaaaat atccaccgca
    13681 ataatatcct gtcaaaaacc atcgtgccag ctcgtatctc agatcaactg gctgataagg
    13741 ctaagaaaat ggcggtgcag attgccaaga aactccagct gtcaggaact ctctgtgtgg
    13801 aaatgtttgc gaccgcagat gatatcattg tcaatgaaat tgccccacgt ccccacaact
    13861 cagggcacta ctcaatcgag gcttgcgact tttcacagtt tgacacccac attttgggcg
    13921 tgctaggagc gccgctccca accatccacc tccatgctcc agccgtcatg ctcaatgtcc
    13981 tcggacaaca cgtccagcag gcgattgact atgtgactga aaaccctagc gcccacctcc
    14041 acatgtatgt taaactagaa gcgaaacata accgcaaaat gggacacgtg acggtgtttg
    14101 cagaagatgc ggatgaggtg gaggagtttt agaacaggag tagattggta agatggagtt
    14161 aagggaattt aaagcgtggt tattaaaaaa ccattttgaa gaagatgagt tatacaagga
    14221 atctataaag tgttatcaag tggaagcata taaggctgcc tatatttttt cgtatttagc
    14281 aaatcataag ttcattgcta aattagctat tgactacagt ggtgtcccat caaattttat
    14341 gtctaaatgt cccgatgatg aagttagaaa gtcgagttgg caaaaacgtg tgaattcctt
    14401 aaaaaatgaa gatgagtggg agcagacggt taacgaaaaa ttaattcaag catgggaagg
    14461 tgataatgta ttcaaattac ctttcaaaat taaagaaaaa tattctcata tgagggtatt
    14521 gcgtaataat gcagcccatg cgaaagactg tcgaatttct gaatcaacag ttttagaatt
    14581 atggaatgaa attgagtata tatatcctta ctttgtaatc aacggaacga ttgatgcatg
    14641 gatggaagat ttagaaaaac tagttagat
//
LOCUS       DAUDYX010000049        14310 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.050, whole genome shotgun sequence.
ACCESSION   DAUDYX010000049 DAUDYX010000000
VERSION     DAUDYX010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14310
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.050"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            55..1602
                     /gene="sfbI"
                     /locus_tag="VHD02_001528"
     CDS             55..1602
                     /gene="sfbI"
                     /locus_tag="VHD02_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein SfbI"
                     /protein_id="HEQ5498124.1"
                     /translation="MVSSYMFARGEKMNNKMFLNKEAGFLVHTKRKRRFAVTLVGVFF
                     LLLACAGAIGFGQVAYAADEKTVPNFKSPDPDYPWYGYDSYRGIFARYHNLKVNLKGS
                     KEYQAYCFNLTKYFPRPTYSTTNNFYKKIDGSGSAFKSYAANPRVLDENLDKLEKNIL
                     NVIYNGYKSNANGFMNGIEDLNAILVTQNAIWYYSDSAPLNDVNKMWEREVRNGEISE
                     SQVTLMREALKKLIDPNLEATAANKIPSGYRLNIFKSENEDYQNLLSAEYVPDDPPKP
                     GDTSEHNPKTPELDGTPIPEDPKRPDESSEPALPPLMPELDGEEVPEVPSESLEPALP
                     PLMPELDGEEVPEKPSVDLPIEVPRYEFNNKDQSPLAGESGETEYITEVYGNQQNPVD
                     IDKKLPNETGFSGNMVETEDTKEPEVLMGGQSESVEFTKDTQTGMSGFSETVTIVEDT
                     RPKLVFHFDNNEPKVEENREKPTKNITPILPATGDIENVLAFLGILILSVLSIFSLLK
                     NKQNNKV"
     gene            1826..2674
                     /locus_tag="VHD02_001529"
                     /pseudo
     CDS             1826..2674
                     /locus_tag="VHD02_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407229.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
     gene            2960..5230
                     /locus_tag="VHD02_001530"
     CDS             2960..5230
                     /locus_tag="VHD02_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus ancillary protein 1"
                     /protein_id="HEQ5498125.1"
                     /translation="MNNKKLQKKQDAPRVSNRKPKQLTVTLVGVFLMFLTLVSSMRGA
                     QSIFGEEKRIEEVSVPKIKSPDDAYPWYGYDSYDSSHPYYERFKVAHDLRVNLNGSKS
                     YQVYCFNINSHYPNRKNAFSKQWFKRVDGTGDVFTNYAQTPKIRGESLNNKLLSIMYN
                     AYPKNANGYMDKIEPLNAILVTQQAVWYYSDSSYGNIKTLWASELKDGKIDFEQVKLM
                     REAYSKLISDDLEETSKNKLPQGSKLNIFVPQDKSVQNLLSAEYVPESPPAPGQSPEP
                     PVQTKKTSVIIRKYAEGDYSKLLEGATLRLTGEDILDFQEKVFQSNGTGEKIELSNGT
                     YTLTETSSPDGYKIAEPIKFRVVNKKVFIVQKDGSQVENPNKEVAEPYSVEAYSDMQD
                     SNYINPETFTPYGKFYYAKNKDKSSQVVYCFNADLHSPPESEDGGGTIDPDISTMKEV
                     KYTHTAGSDLFKYALRPRDTNPEDFLKHIKKVIEKGYNKKGDSYNGLTETQFRAATQL
                     AIYYFTDSTDLKTLKTYNNGKGYHGFESMDEKTLAVTKELINYAQDNSAPQLTNLDFF
                     VPNNSKYQSLIGTEYHPDDLVDVIRMEDKKQEVIPVTHSLTVKKTVVGELGDKTKGFQ
                     FELELKDKTGQPIVNTLKTNNQDLVAKDGKYSFNLKHGDTIRIEGLPTGYSYTLKETE
                     AKDYIVTVDNKVSQEAQSASENVTADKEVTFENRKDLVPPTGFITDGGTYLWLLLLVP
                     FGLLVWFFGRKGLKND"
     gene            5223..5744
                     /gene="lepB"
                     /locus_tag="VHD02_001531"
     CDS             5223..5744
                     /gene="lepB"
                     /locus_tag="VHD02_001531"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEQ5498126.1"
                     /translation="MTNYLNRLNENPLFKAFIRLVLKISIIGFLGYILFQYVFGVMIV
                     NTNQMSPAVSAGDGVLYYRLTDRYHINDVVVYEVDNTLKVGRIAAQAGDEVSFTQEGG
                     LLINGHSPEKEVPYLTYPHSSGPNFPYKVPTGTYFILNDYREERLDSRYYGALPINQI
                     KGKISTLLRVRGI"
     gene            5766..6794
                     /locus_tag="VHD02_001532"
     CDS             5766..6794
                     /locus_tag="VHD02_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QVPTGV class sortase B protein-sorting
                     domain-containing protein"
                     /protein_id="HEQ5498127.1"
                     /translation="MKKNKLLLATAILATALGTASLNQNVKAETAGVVSSGQLTIKKS
                     ITNFNDDTLLMPKTDYTFSVNPDSAATGTESNLPIKPGIAVNNQDIKVSYSNTDKTSG
                     KEKQVVVDFMKVTFPSVGIYRYVVTENKGTAEGVTYDDTKWLVDVYVGNNEKGGLEPK
                     YIVSKKGDSATKEPIQFNNSFETTSLKIEKEVTGNTGDHKKAFNFTLTLQPNEYYEAS
                     SVVKIEENGQTKDVKIGEAYKFTLNDSQSVILSKLPVGINYKVEEAEANQGGYTTTAT
                     LKDGEKLSTYNLGQEHKTDKTADEIVVTNNRDTQVPTGVVGTLAPFAVLSIVAIGGVI
                     YITKRKKA"
     gene            6810..7535
                     /gene="srtB"
                     /locus_tag="VHD02_001533"
     CDS             6810..7535
                     /gene="srtB"
                     /locus_tag="VHD02_001533"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HEQ5498128.1"
                     /translation="MMMTIVQVINKAIDTLILIFCLVVLFLAGFGLWDSYHLYQQADA
                     SNFKKFKTAQQQPKFEDLLALNEDVIGWLNIPGTHIDYPLVQGKTNLEYINKAVDGSV
                     AMSGSLFLDTRNHNDFTDDYSLIYGHHMAGNAMFGEIPKFLKKDFFNKHNKAIIETKE
                     RKKLTVTIFACLKTDAFDQLVFNPNAITNQDQQRQLVDYISKRSKQFKPVKLKHHTKF
                     VAFSTCENFSTDNRVIVVGTIQE"
     gene            7552..8139
                     /locus_tag="VHD02_001534"
     CDS             7552..8139
                     /locus_tag="VHD02_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEQ5498129.1"
                     /translation="MRKYWKMLFSVVMILTMLAFNQTVLAKDSTVQTSISVENVLERA
                     GDSTPFSIALESIDAMKTIEEITIAGSGKASFSPLTFTTVGQYTYRVYQKPSQNKDYQ
                     ADTTVFDVLVYVTYDEDGTLVAKVISRRAGDEEKSAITFKPKRLVKPIPPRQPNIPKT
                     PLPLAGEVKSLLGILSIVLLGLLVLLYVKKLKSKL"
     gene            complement(8298..9503)
                     /locus_tag="VHD02_001535"
     CDS             complement(8298..9503)
                     /locus_tag="VHD02_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEQ5498130.1"
                     /translation="MVIFDLKHVQTLHSLSQLPISVMSQDKALIQVYGNDDYLLCYYQ
                     FLKHLAIPQAAQDVIFYEGLFEESFMIFPLCHYIIAIGPFYPYSLNKDYQEQLANNFL
                     KHSSHRSKEELLSYMALVPHFPINNVRNLLIAIDAFFDTQFETTCQQTIHQLLQHSKQ
                     MTADPDIIHRLKHISKASSQLPPVLEHLNHIMDLVKLGNPQLLKQEINRIPLSSITSS
                     SISALRAEKNLTVIYLTRLLEFSFVENTDVAKHYSLVKYYMALNEEASDLLKVLRIRC
                     AAIIHFSESLTNKSISDKRQMYNSVLHYVDSHLYSKLKVSDIAKRLYVSESHLRSVFK
                     KYSNVSLQHYILSTKIKEAQLLLKRGIPVGEVAKSLYFYDTTHFHKIFKKYTGISSKD
                     YLAKYRDNI"
     gene            9888..13373
                     /locus_tag="VHD02_001536"
     CDS             9888..13373
                     /locus_tag="VHD02_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEQ5498131.1"
                     /translation="MTQKNSYKLSFLLSLTGFILGLLLVFIGLSGVSVGHAETRNGAN
                     KQGSFEIKKVDQNNKPLPGATFSLTSKDGKGTSVQTFTSNDKGIVDAQNLQPGTYTLK
                     EETAPDGYDKTSRTWTVTVYENGYTKLVENPYNGEIISKAGSKDVSSSLQLENPKMSV
                     VSKYGKTEVSSGAADFYRNHAAYFKMSFELKQKDKSETINPGDTFVLQLDRRLNPKGI
                     SQDIPKIIYDSANSPLAIGKYHAENHQLIYTFTDYIAGLDKVQLSAELSLFLENKEVL
                     ENTSISNFKSTIGGQEITYKGTVNVLYGNESTKESNYITNGLSNVGGSIESYNTETGE
                     FVWYVYVNPNRTNIPYATMNLWGFGRARSNTSDLENDANTSSAELGEIQVYEVPEGEK
                     LPSSYGVDVTKLTLRTDITAGLGNGFQMTKRQRIDFGNNIQNKAFIIKVTGKTDQSGK
                     PLVVQSNLASFRGASEYAAFTPVGGNVYFQNEIALSPSKGSGSGKSEFTKPSITVANL
                     KRVAQLRFKKMSTDNVPLPEAAFELRSSNGNSQKLEASSNTQGEVHFKDLTSGTYDLY
                     ETKAPKGYQQVTEKLATVTVDTTKPAEEMVTWGSPHSSVKVEANKEVTIVNHKETLTF
                     SGKKIWENDRPDQRPAKIQVQLLQNGQKMPNQIQEVTKDNDWSYHFKDLPKYDAKNQE
                     YKYSVEEVNVPDGYKVSYLGNDIFNTRETEFVFEQNNFNLEFGNAEIKGQSGSKIIDE
                     DTLTSFKGKKIWKNDTAENRPQAIQVQLYADGVAVEGQTKFISGSGNEWSFEFKNLKK
                     YNGTGNDIIYSVKEVTVPTGYDVTYSANDIINTKREVITQQGPKLEIEETLPLESGAS
                     GGTTTVEDSRPVDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDIDGKELAGATMELR
                     DSSGKTISTWISDGQVKDFYLMPGKYTFVETAAPDGYEIATAITFTVNEQGQVTVNGK
                     ATKGDTHIVMVDAYKPTKGSGQVIDIEEKLPDEQGHSGSTTEIEDSKSSDLIIGGQGE
                     VVDTTEDTQSGMTGHSGSTTEIEDSKSSDVIIGGQGQVVETTEDTQTGMYGDSGCKTE
                     VEDTKLVQSFHFDNKEPESNSEIPKKDKPKSNTSLPATGEKQHNMFFWMVTSCSLISS
                     VFVISLKSKKRLSSC"
     gene            complement(13640..14305)
                     /locus_tag="VHD02_001537"
     CDS             complement(13640..14305)
                     /locus_tag="VHD02_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498132.1"
                     /translation="MKRCNKYLFTSLLAASMLFSSYKSVHAHDNIDEKGKVHLYWQGN
                     YYVDNYVDYTKALTADEKSIEWTVTFNSAKEQWVYPDFSVFLPKGVKAPTEITYEHHY
                     WDGTVHSEKHNNTQWHYDWKSQQTNFNQEFDKFPGYTGWSPSLDKFYKLKNDGKFSHV
                     LVDTYGRQSHTYFSHKMVWKFKTELEEDYKNKWDKLPFIAGIKQNNPLAASFPSYKGE
                     FGE"
BASE COUNT         4927 a         2325 c         2859 g         4199 t
ORIGIN      
        1 aaacattaat ttggtgaaat atacaatcaa aaaaatatct ttcttgacaa taacatggta
       61 agctcatata tgtttgcgag aggagagaaa atgaataaca aaatgttttt gaacaaagaa
      121 gccggttttt tggtacacac aaaaagaaaa aggcgatttg ctgtcacttt agtgggagtc
      181 ttttttctgc ttttggcatg tgcgggtgct atcggttttg gtcaagtagc ctatgctgcg
      241 gatgagaaga ctgtgccgaa ttttaaaagc ccagatccag attatccctg gtatggttat
      301 gattcgtata gaggaatatt tgcaagatat cacaatttaa aagtaaatct aaaaggaagt
      361 aaggagtatc aagcgtattg ttttaaccta acaaaatact ttcctcgccc cacttatagt
      421 actacaaata atttttacaa gaaaattgat gggagtggat cagcgttcaa atcttatgca
      481 gcgaatccta gggttttaga tgagaattta gataaattag aaaaaaatat actgaatgta
      541 atttataatg gatataaaag taatgcaaat ggttttatga atggtataga agatcttaat
      601 gctatactag taactcaaaa cgctatttgg tactattcag atagtgctcc attaaatgat
      661 gttaataaaa tgtgggaaag agaggttcgg aatggggaga ttagtgagtc acaagttact
      721 ttaatgcgtg aggcattgaa aaaactaatt gatcccaatt tagaagctac tgcagctaat
      781 aaaatcccat caggatatcg tttaaatatc tttaagtctg aaaatgaaga ttaccaaaat
      841 cttttaagtg ctgaatatgt acctgatgat ccccctaaac ctggtgatac gtcagaacat
      901 aatcctaaaa ctcccgagtt ggatggcact ccaattcccg aggacccaaa acgtccagat
      961 gagagttcag aacctgcgct tcccccatta atgccagagc tagatggtga agaagtccca
     1021 gaagttccaa gcgagagctt agaacctgcg cttcccccat tgatgccaga gttagatggt
     1081 gaagaagtcc ctgaaaaacc tagtgttgac ttacctattg aagttcctcg ttatgagttt
     1141 aacaataaag accagtcacc tctagcgggt gagtctggtg agacggagta tattaccgaa
     1201 gtctatggaa atcaacagaa ccctgttgat attgataaaa aacttccgaa tgaaacaggt
     1261 ttttcaggaa atatggttga gacagaagat acgaaagagc cagaagtgtt gatgggaggt
     1321 caaagtgagt ctgttgaatt tactaaagac actcaaacag gcatgagcgg tttcagtgaa
     1381 acagtgacca ttgttgaaga tacgcgtccg aagttagtgt tccattttga caataatgag
     1441 cccaaagtgg aagagaatcg ggaaaagcct acaaaaaata taacacctat ccttcctgca
     1501 acaggagata ttgagaatgt tttggccttt cttggaatcc ttattttgtc agtactttct
     1561 atttttagcc ttttaaaaaa caaacaaaac aataaagtct gacgtaaaag tgttccataa
     1621 gaaataatgc gcaaatatgc tgaatagtgg ataactgcta cagtcaagac aagtgttaat
     1681 tcaaaaggta ctacttatac atcgtataat agacttacaa gaaatcaagt tattgttaaa
     1741 cgtcgtaaag cttaacgctt tcaaaagtcg aaatcaatcg agactgtctt taggcagtct
     1801 cgatttttat tgataaggaa ctgcaatgac agaaagacta aaaaatctag ggatactctt
     1861 attattttta ttgggaacag ccatttttct ttaccctacg ctaagtagtc agtggaatgc
     1921 ctatcgtgat cgtcaattgt tatcaactta tcacaaacaa gtgactcaaa agaagccctc
     1981 agaaatggag gaggtgtggc aaaaggctaa ggcgtataac gctaggcttg ggattcagcc
     2041 cgttccagat gctttttcat ttagggatgg cattcatgat aaaaactacg aaagcctact
     2101 tcaaattgaa aacaatgata ttatgggata agttgaagtg ccctctatta aagtgacgtt
     2161 acccatatac cactatacga cagacgaggt gctaactaag ggagcaggtc acttagttgg
     2221 aagtgcctta ccagttggtg gcgacgggac tcacacggtt atttctgctc atagaggcct
     2281 tccatcggca gaaatgttta ctaatttgaa tttagtgaaa aggggagata ccttttactt
     2341 tcgtgtgtta aataaagtat tggcttataa agttgatcag atactaactg ttgaaccaga
     2401 tcaagtcaca tctctttctg gggtaatggg aaaagatcat gctactctgg ttacctgcac
     2461 accatatggt gttaatacca agcgtctatt ggtcagaggt catagaatta catatcatta
     2521 caaaaagtat caacaggcta aaaaagcaat gaaactcgtg gataaatcaa gaatgtggac
     2581 agaagtagtg tgtgctgctt tcggggtggt tatagctatt attttagtat ttatgtattc
     2641 aaagatgact gataaaaaag tgattaaaaa ctaacttgcc ctgaaccttc tttatcgtat
     2701 tgaaactgtc ataaaagtaa aataatctgg tagttactat cccctttgat gaagtttaat
     2761 taaatctcat caagagaaaa atattaggcg attacaaatg attttcccaa aaattatttt
     2821 ttggaacgca taatatggaa aaaaagatga taagaacaac tgttttaaac atcaaataaa
     2881 tgatatagta gctatgaaat agcattgtga ctgtccggta aagggatggg actgaagatt
     2941 ttattcagaa ggagagggaa tgaataacaa aaaattgcaa aagaagcaag atgctcctcg
     3001 ggtatcaaac agaaagccaa aacaattaac tgtcacttta gtgggagtat ttttaatgtt
     3061 tttgaccttg gtaagttcca tgagaggtgc tcaaagcata tttggagagg aaaagagaat
     3121 tgaagaagtc agtgttccta aaataaaaag tccagatgat gcctaccctt ggtatggcta
     3181 tgattcatat gactctagtc atccttacta tgaacgtttt aaagtagcac atgatttaag
     3241 ggttaattta aatggaagta agagctacca agtatattgc tttaatatca attctcatta
     3301 tccgaataga aaaaatgctt tttctaaaca atggtttaag agagttgatg ggacaggtga
     3361 tgtgttcaca aattatgctc agacacctaa gattcgtgga gaatcattga ataataaact
     3421 tttaagtatt atgtacaacg cttatcctaa aaatgctaat ggctatatgg ataagataga
     3481 accattaaat gctattttag taactcaaca agctgtttgg tactattctg acagttctta
     3541 tggtaatata aaaacgttat gggcatctga gcttaaagac ggaaaaatag attttgaaca
     3601 agtaaaatta atgcgtgaag cttactcaaa actaattagt gatgatttag aagaaacatc
     3661 taaaaataag ctacctcaag gatctaaact gaatattttt gttccgcaag ataaatctgt
     3721 tcaaaattta ttaagtgcag agtacgtgcc tgaatcccct ccggcaccag gtcagtctcc
     3781 agaaccgcca gtgcaaacaa aaaaaacatc agtcattatc agaaaatatg cggaaggtga
     3841 ctactctaaa cttctagagg gagcaacttt gcgtttaaca ggggaagata tcctagattt
     3901 tcaagaaaaa gtcttccaaa gtaatggaac aggagaaaag attgaattat caaatgggac
     3961 ttatacctta acagaaacat catctccaga tggatataaa attgcggagc cgattaagtt
     4021 tagagtagtg aataaaaaag tatttatcgt ccaaaaagat ggttctcaag tggaaaatcc
     4081 aaacaaagaa gtagcagagc catactcagt ggaagcgtac agcgatatgc aagatagtaa
     4141 ctatattaat ccagaaacgt tcactcctta tgggaaattt tattacgcta aaaataagga
     4201 taaaagttca caagttgtct actgttttaa tgctgattta cactctccac ctgaatcaga
     4261 ggatggggga ggaactatag atcctgatat tagtacgatg aaagaagtca agtacacaca
     4321 tacggcaggt agtgatttgt ttaaatacgc gctaagaccg agagatacaa atccagaaga
     4381 cttcttaaag cacattaaaa aagtaattga aaaaggctac aataaaaaag gtgatagcta
     4441 taatggatta acagaaacac agtttcgcgc ggctactcag cttgctatct attactttac
     4501 agacagcact gacttaaaaa ccttaaaaac ttataacaat gggaaaggtt accatggatt
     4561 tgaatctatg gatgaaaaaa ccctagctgt aacaaaagaa ttaattaatt acgctcaaga
     4621 taatagtgcc cctcaactaa caaatcttga tttcttcgta cctaataata gcaaatacca
     4681 atctcttatt gggacagaat accatccaga tgatttggtt gacgtgattc gtatggaaga
     4741 taaaaagcaa gaagttattc cagtaactca cagtttgaca gtgaaaaaaa cagtagtcgg
     4801 tgagttggga gataaaacta aaggcttcca atttgaactt gagttgaaag ataaaactgg
     4861 acagcctatt gttaacactc taaaaactaa taatcaagat ttagtagcta aagatgggaa
     4921 atattcattt aatctaaagc atggtgacac cataagaata gaaggattac cgacgggata
     4981 ttcttatact ctgaaagaga ctgaagctaa ggattatata gtaaccgttg ataacaaagt
     5041 tagtcaagaa gctcaatcag caagtgagaa tgtcacagca gacaaagaag tcacttttga
     5101 aaaccgtaaa gatcttgtcc caccaactgg ttttattact gatggtggaa cctatctgtg
     5161 gttattattg cttgtcccat ttggtttgtt agtgtggttc tttggtcgta aaggactaaa
     5221 aaatgactaa ttacctaaat cgcttaaatg agaatccact atttaaagct ttcatacggt
     5281 tagtacttaa gatttctatt attggatttc taggttacat tctatttcag tatgtttttg
     5341 gcgtcatgat tgttaacaca aatcagatga gtcctgctgt aagtgctggt gatggagtct
     5401 tatattatcg tttgactgat cgctatcata ttaatgatgt ggtggtctat gaggttgata
     5461 acactttgaa agttggtcga attgccgctc aagctggcga tgaggttagt tttacgcaag
     5521 aaggaggact gttgattaat gggcattcac cagaaaaaga ggtcccttac ctgacgtatc
     5581 ctcactcaag tggtccaaac tttccctata aagttcctac gggtacgtat ttcatattga
     5641 atgattatcg tgaagaacgt ttggacagtc gttattatgg ggcgttaccc atcaatcaaa
     5701 tcaaagggaa aatctcaact ctattaagag tgagaggaat ttaaaaaaat ttggagagag
     5761 agaaaatgaa aaaaaataaa ttattacttg ctactgcaat cttagcaact gctttaggaa
     5821 cagcttcttt aaatcaaaac gtaaaagctg agacggcagg ggttgttagc agtggtcaat
     5881 taacaataaa aaaatcaatt acaaatttta atgatgatac acttttgatg cctaagacag
     5941 actatacttt tagcgttaat ccggatagtg cggctacagg tactgaaagt aatttaccaa
     6001 ttaaaccagg tattgctgtt aacaatcaag atattaaggt ttcttattct aatactgata
     6061 agacatcagg taaagaaaaa caagttgttg ttgactttat gaaagttact tttcctagcg
     6121 ttggtattta ccgttatgtt gttaccgaga ataaagggac agcagaagga gttacatatg
     6181 atgatacaaa atggttagtt gacgtctatg ttggtaataa tgaaaaggga ggtcttgaac
     6241 caaagtatat tgtatctaaa aaaggagatt ctgctactaa agaaccaatc cagtttaata
     6301 attcattcga aacaacgtca ttaaaaattg aaaaggaagt tactggtaat acaggagatc
     6361 ataaaaaagc atttaacttt acattaacat tgcaaccaaa tgaatactat gaggcaagtt
     6421 cggttgtgaa aattgaagag aacggacaaa cgaaagatgt gaaaattggg gaggcatata
     6481 agtttacttt gaacgatagt cagagtgtga tattgtctaa attaccagtt ggtattaatt
     6541 ataaagttga agaagcagaa gctaatcaag gtggatatac tacaacagca actttaaaag
     6601 atggagaaaa gttatctact tataacttag gtcaggaaca taaaacagac aagactgctg
     6661 atgaaatcgt tgtcacaaat aaccgtgaca ctcaagttcc aactggtgtt gtaggcaccc
     6721 ttgctccatt tgcagttctt agcattgtgg ctattggtgg agttatctat attacaaaac
     6781 gtaaaaaagc ttaagtgatt agtcaaagaa tgatgatgac aattgtacag gttatcaata
     6841 aagccattga tactctcatt cttatctttt gtttagtcgt actattttta gctggttttg
     6901 gtttgtggga ttcttatcat ctctatcaac aagcagacgc ttctaatttc aaaaaattta
     6961 aaacagctca acaacagcct aaatttgaag acttgttagc tttgaatgag gatgtcattg
     7021 gttggttaaa tatcccgggg actcatattg attatcctct agttcaggga aaaacgaatt
     7081 tagagtatat taataaagca gttgatggca gtgttgccat gtctggtagt ttatttttag
     7141 atacacggaa tcataatgat tttacggacg attactctct gatttatggc catcatatgg
     7201 caggtaatgc catgtttggc gaaattccaa aatttttaaa aaaggatttt ttcaacaaac
     7261 ataataaagc tatcattgaa acaaaagaga gaaaaaaact aaccgtcact atttttgctt
     7321 gtctcaagac agatgccttt gaccagttag tttttaatcc taatgctatt accaatcaag
     7381 accaacaaag gcagctcgtt gattatatca gtaaaagatc aaaacaattt aaacctgtta
     7441 aattgaagca tcatacaaag ttcgttgctt tttcaacgtg tgaaaatttt tctactgaca
     7501 atcgtgttat cgttgtcggt actattcaag aataacgaaa ggaggagact tttgagaaaa
     7561 tattggaaaa tgttattttc tgtcgtaatg atattaacca tgctggcctt taatcagact
     7621 gttttagcaa aagacagcac tgttcaaact agcattagtg tcgaaaatgt cttagagaga
     7681 gcaggcgata gtaccccatt ttcgattgca ttagaatcaa ttgatgcgat gaaaacaata
     7741 gaagaaataa caattgctgg ttctggaaaa gcaagctttt cccctctgac cttcacaaca
     7801 gttgggcaat atacttatcg tgtttatcag aagccttcac aaaataaaga ttatcaagca
     7861 gatactactg tatttgacgt tcttgtctat gtgacctatg atgaagatgg gactctagtc
     7921 gcaaaagtta tttctcgaag ggctggagac gaagaaaaat cagcgattac ttttaagccc
     7981 aaacggttag taaaaccaat accgcctaga caacctaaca tccctaaaac cccattacca
     8041 ttagctggtg aagtaaaaag tttattgggt atcttaagta tcgtattact ggggttacta
     8101 gttcttcttt atgttaaaaa actgaagagt aagctataaa acccattttt aagagtcaca
     8161 catttttagt tgctctattt ttataatgaa acttattgac atcttcatag tcttgcttga
     8221 ctttggagat gttttctatg ctgataggtt tttgtaccat ataagataat gattgatgaa
     8281 aagagtgtct tgaattctta aatattatct cggtatttag caagatagtc ttttgaagaa
     8341 atacccgtgt attttttaaa gattttatga aaatgggtag tgtcataaaa atataagctt
     8401 ttagccactt ctccaacagg aattcctcgt tttaagagta gttgagcttc tttgattttt
     8461 gtacttagaa tataatgttg taaggaaaca tttgagtatt ttttaaagac tgaacgtaag
     8521 tgagattcgg aaacatatag gcgcttagcg atatcagata cctttaattt ggaatacagg
     8581 tgactatcga cataatgaag cacactattg tacatttgac gtttatcaga aatactttta
     8641 ttggttaatg attcggaaaa atggatgatg gctgcacagc gaattctcaa aactttgagc
     8701 aagtcactcg cttcttcatt taaggccatg tagtatttga caaggctata atgctttgct
     8761 acgtcagtat tttctacaaa actgaattcc agtaacctag ttaaatagat aacagtgagg
     8821 ttcttttccg cccttagagc agaaatagaa gatgaggtga tacttgataa ggggatgcga
     8881 ttgatttctt gcttgagcaa ttgtggattg cctagcttta ccagatccat aatatgattt
     8941 aggtgctcta aaacaggcgg tagttggcta gatgctttgc taatatgctt aaggcgatga
     9001 atgatatcag gatcagcagt catctgtttt gaatgctgca acaattgatg aattgtttgt
     9061 tggcaagtcg tctcaaattg tgtgtcaaaa aaagcgtcaa tagctatcaa aaggttccgc
     9121 acattattaa ttggaaaatg tgggacaagt gccatatagg ataagagctc ttctttgcta
     9181 cgatgagaag aatgttttaa aaaattatta gctaattgtt cctgatagtc tttattaagt
     9241 gaataagggt agaaaggtcc aatggcaata atgtagtgac aaagaggaaa aatcataaag
     9301 gactcttcaa ataaaccctc ataaaaaata acatcttgtg cagcttgagg aatagctaga
     9361 tgctttaaaa attgatagta acataataaa tagtcgtcat taccatatac ttgaataagt
     9421 gccttatctt gtgacatcac tgaaataggt aattgagaca agctgtgtaa tgtttgcaca
     9481 tgttttaaat cgaatataac catttgatag attgcccccc atccttagtt gtatcatttt
     9541 ccttagactt gtaaaagctt tagtaaatgt taatggtgaa aattcctatt tggtaggcac
     9601 actaaaaggt cttcctaata gtaaactctc tctccatcat ctcctgcctt aattatacta
     9661 tgaatagcta agcagatcaa gataaaggat ttgtacgtta agcgcttgaa aaagaaagag
     9721 ttacagataa tgacataaaa aacgctaaaa aaccatcaaa ataaatactc tgaccataag
     9781 atgtagactt gacaactgaa aatggtaaaa taactatttg acagttggcc tgtagtcttt
     9841 agttttggac ataggctgtc gcttatgaat gtggagagag aaaataaatg acacaaaaaa
     9901 atagctataa gttaagcttc ctgttatccc taacaggatt tattttaggt ttattattgg
     9961 tttttatagg attgtccgga gtatcagtag gacatgcgga aacaagaaat ggagcaaaca
    10021 aacaaggatc ttttgaaatc aagaaagtcg accaaaacaa taagccttta ccgggagcaa
    10081 ctttttcact gacatcaaag gatggcaagg gaacatctgt tcaaacgttc acttcaaatg
    10141 ataaaggtat tgtagatgct caaaatctcc aaccagggac ttatacctta aaagaagaaa
    10201 cagcaccaga tggttatgat aaaaccagcc ggacttggac agtgactgtt tatgagaacg
    10261 gctataccaa gttggttgaa aatccctata atggggaaat catcagtaaa gcagggtcaa
    10321 aagatgttag tagttcttta cagttggaaa atcccaaaat gtcagttgtt tctaaatatg
    10381 ggaaaacaga ggttagtagt ggcgcagcgg atttctaccg caaccatgcc gcctatttta
    10441 aaatgtcttt tgagttgaaa caaaaggata aatctgaaac aatcaaccca ggtgatacct
    10501 ttgtgttaca gctggataga cgtctcaatc ctaaaggtat cagtcaagat atccctaaaa
    10561 tcatttacga cagtgcaaat agtccgcttg cgattggaaa ataccatgct gagaaccatc
    10621 aacttatcta tactttcaca gattatattg cgggtttaga taaagtccag ttgtctgcag
    10681 aattgagctt attcctagag aataaggaag tgttggaaaa tactagtatc tcaaatttta
    10741 agagtaccat aggtgggcag gagatcacct ataaaggaac ggttaatgtt ctttatggaa
    10801 atgagagcac taaagaaagc aattatatta ctaatggatt gagcaatgtg ggtgggagta
    10861 ttgaaagcta caacaccgaa acgggagaat ttgtctggta tgtttatgtc aatccaaacc
    10921 gtaccaatat tccttatgcg accatgaatt tatggggatt tggaagggct cgttcaaata
    10981 caagcgactt agaaaacgac gctaatacaa gtagtgctga gcttggagag attcaggtct
    11041 atgaagtacc tgaaggagaa aaattaccat caagttatgg ggttgatgtt acaaaactta
    11101 ctttaagaac ggatatcaca gcaggcctag gaaatggttt tcaaatgacc aaacgtcagc
    11161 gaattgactt tggaaataat atccaaaata aagcatttat catcaaagta acagggaaaa
    11221 cagaccaatc tggtaagcca ttggttgttc aatccaattt ggcaagtttt cgtggtgctt
    11281 ctgaatatgc tgcttttact ccagttggag gaaatgtcta cttccaaaac gaaattgcct
    11341 tgtctccttc taagggtagt ggttctggga aaagtgaatt tactaagccc tctattacag
    11401 tagcaaatct aaaacgagtg gctcagcttc gctttaagaa aatgtcaact gacaatgtgc
    11461 cattgccaga agcggctttt gagctgcgtt catcaaatgg taatagtcag aaattagaag
    11521 ccagttcaaa cacacaagga gaggttcact ttaaggacct gacctcgggc acatatgacc
    11581 tgtatgaaac aaaagcgcca aaaggttatc agcaggtgac agagaaattg gcgaccgtta
    11641 ctgttgatac taccaaacct gctgaggaaa tggtcacttg gggaagccca cattcgtctg
    11701 taaaagtaga agctaacaaa gaagtcacga ttgtcaacca taaagaaacc cttacgtttt
    11761 cagggaagaa aatttgggag aatgacagac cagatcaacg cccagcaaag attcaagtgc
    11821 aactgttgca aaatggtcaa aagatgccta accagattca agaagtaacg aaggataacg
    11881 attggtctta tcacttcaaa gacttgccta agtacgatgc caagaatcag gagtataagt
    11941 actcagttga agaagtaaat gttccagacg gctacaaggt gtcgtattta ggaaatgata
    12001 tatttaacac cagagaaaca gaatttgtgt ttgaacagaa taactttaac cttgaatttg
    12061 gaaatgctga aataaaaggt caatctgggt caaaaatcat tgatgaagac acgctaacgt
    12121 ctttcaaagg taagaaaatt tggaaaaatg atacggcaga aaatcgtccc caagccattc
    12181 aagtgcagct ttatgctgat ggagtggctg tggaaggtca aaccaaattt atttctggct
    12241 caggtaatga gtggtcattt gagtttaaaa acttgaagaa gtataatgga acaggtaatg
    12301 acatcattta ctcagttaaa gaagtaactg ttccaacagg ttatgatgtg acttactcag
    12361 ctaatgatat tattaatacc aaacgtgagg ttattacaca acaaggaccg aaactagaga
    12421 ttgaagaaac gcttccgcta gaatcaggtg cttcaggcgg taccactact gtcgaagact
    12481 cacgcccagt tgatacctta tcaggtttat caagtgagca aggtcagtcc ggtgatatga
    12541 caattgaaga agatagtgct acccatatta aattctcaaa acgtgatatt gacggcaaag
    12601 agttagctgg tgcaactatg gagttgcgtg attcatctgg taaaactatt agtacatgga
    12661 tttcagatgg acaagtgaaa gatttctacc tgatgccagg aaaatataca tttgtcgaaa
    12721 ccgcagcacc agacggttat gagatagcaa ctgctattac ctttacagtt aatgagcaag
    12781 gtcaggttac tgtaaatggc aaagcaacta aaggtgacac tcatattgtc atggttgatg
    12841 cttacaagcc aactaagggt tcaggtcagg ttattgatat tgaagaaaag cttccagacg
    12901 agcaaggtca ttctggttca actactgaaa tagaagacag taaatcttca gaccttatca
    12961 ttggcggtca aggtgaagtt gttgacacaa cagaagacac acaaagtggt atgacgggcc
    13021 attctggctc aactactgaa atagaagata gcaagtcttc agacgttatc attggtggtc
    13081 aggggcaggt tgtcgagaca acagaggata cccaaactgg catgtacggg gattctggtt
    13141 gtaaaacgga agtcgaagat actaaactag tacaatcctt ccactttgat aacaaggaac
    13201 cagaaagtaa ctctgagatt cctaaaaaag ataagccaaa gagtaatact agtttaccag
    13261 caactggtga gaagcaacat aatatgttct tttggatggt tacttcttgc tcacttatta
    13321 gtagtgtttt tgtaatatca ctaaaatcca aaaaacgcct atcatcatgt taagataagc
    13381 tgttggtgac aatagcgaaa aagggtccac ttattagtgg gcctcttttt ctggaatcga
    13441 tcgtctcggt tcactagata agtcatttta agtagttcaa aaaggccgag ccttaaaagg
    13501 ttttctgcct ttttaatctt gttgggtact tacgaaagta tttcggccac ttcaagcata
    13561 atacagtcag agtctgagca atcatgttgt ctcagactct gactgtatta tgcaaaagat
    13621 gatggactgg cagcttcatt tactcaccga actccccttt ataacttggg aatgatgctg
    13681 ctaatgggtt gttttgttta atgcctgcta taaacggtaa tttatcccat ttatttttat
    13741 aatcttcttc aagctcagtt ttaaacttcc aaaccatttt atgagaaaaa taagtgtgtg
    13801 attgacggcc ataggtgtct actaaaacgt gagaaaattt tccgtcgttt tttagtttgt
    13861 aaaatttatc tagagaagga ctccagccag tataaccagg gaatttatca aattcttgat
    13921 taaaatttgt ttgttggctt ttccaatcat aatgccattg tgtattattg tgtttttcag
    13981 aatgaactgt accgtcccaa taatgatgct cataagttat ctctgtagga gcttttacac
    14041 ccttaggaag aaaaactgag aaatcagggt aaacccattg ttctttagct gaattaaagg
    14101 ttacagtcca ttcgatactt ttttcatctg cagttaatgc tttagtatag tcgacatagt
    14161 tatcaacata gtaatttccc tgccagtaaa ggtgcacttt acctttctca tcaatgttat
    14221 catgggcatg tactgattta tacgaactaa acagcatcga agctgctagt agtgaagtga
    14281 agagatattt attacatcgt ttcatatcat
//
LOCUS       DAUDYX010000050        13642 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.051, whole genome shotgun sequence.
ACCESSION   DAUDYX010000050 DAUDYX010000000
VERSION     DAUDYX010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13642
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.051"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(306..1751)
                     /locus_tag="VHD02_001538"
     CDS             complement(306..1751)
                     /locus_tag="VHD02_001538"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEQ5498133.1"
                     /translation="MSKPIRVRYAPSPTGLLHIGNARTALFNYLYARRHGGTFIIRIE
                     DTDRKRHVEDGERSQLENLKWLGMDWDESPETHENYRQSERLALYQQYIDQLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEFIGMSADEKAKYIAEREAAGIVPTVRLAV
                     NESGIYKWTDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVVDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     PEAVFNFIALLGWNPGGEEEIFSREQLIALFDENRLSKSPAAFDQKKMDWMSNEYLKH
                     ADFETVYALCKPFLEEAGRLTEKAEKLVELYKPQLKSADEIIPLTDLFFSDFPELTEA
                     EKEVMAGETVSTVLQAFKAKLEAMSDEDFKPENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPNTIYLLGRDKSIEHIKNML"
     gene            complement(1933..2643)
                     /locus_tag="VHD02_001539"
     CDS             complement(1933..2643)
                     /locus_tag="VHD02_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEQ5498134.1"
                     /translation="MVDNRMRFTIDQNMQFPLVEIDLGHGGSVYLQQGSMVYHTENVT
                     LNTKLNGKGSGLGKLVGAIGRSMVSGESMFITQAMSNGDGKLALAPNTPGQIIALELG
                     EKQYRLNDGAFLALDGSAQYKMERQNIGKALFGGQGGLFVMTTEGLGTLLANSFGSIK
                     KITLDGGTMTIDNAHVVAWSRELDYDIHLENGFMQSIGTGEGVVNTFRGHGEIYIQSL
                     NLEQFAGTLKRYLPTSSN"
     gene            complement(2774..3271)
                     /locus_tag="VHD02_001540"
     CDS             complement(2774..3271)
                     /locus_tag="VHD02_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEQ5498135.1"
                     /translation="MSYFEHFMAANQAYVALHGTAHLPLKPKTKVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFTNEGF
                     AKHIHEHLGMDVSGQDFLPFQDVEDSVREDMTKIRASSLISDDVVINGAVYDVDTGKM
                     TQVME"
     gene            complement(3460..4821)
                     /gene="radA"
                     /locus_tag="VHD02_001541"
     CDS             complement(3460..4821)
                     /gene="radA"
                     /locus_tag="VHD02_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEQ5498136.1"
                     /translation="MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKN
                     ARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRVLGGGVVPGSLILIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYASGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
                     NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNARRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGE
                     IGLTGEIRRVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIEVVGVTTVGQVLKA
                     VFS"
     gene            complement(4902..5348)
                     /locus_tag="VHD02_001542"
     CDS             complement(4902..5348)
                     /locus_tag="VHD02_001542"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEQ5498137.1"
                     /translation="MTKIRGFELVSSFTNPDLLPKRETTHAAGYDLSVAEAVTIAPGE
                     IKLVPTGVKAYMQDGEVLYLYDRSSNPRKKGIILINSVGVIDADYYGNEANEGHIFAQ
                     MQNITDHPVTLAVGERIVQGVFMPFLIADGDQARGERTGGFGSTGQ"
     gene            complement(5458..6225)
                     /locus_tag="VHD02_001543"
     CDS             complement(5458..6225)
                     /locus_tag="VHD02_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEQ5498138.1"
                     /translation="MIDLQEILANMNPNQKINYDRVMQQMAKVWEKESVRPSILMHVC
                     CAPCSTYTLEYLTQFADITVYFANSNIHPKDEYHRRAYVTQQFVSEFNAKTGNTVQFL
                     EADYVPNEYVRQVRGLEEEPEGGDRCRVCFDYRLDKTAQKAVELGFDYFASALTISPH
                     KNSQTINDVGIDVQKVYTTKYLPSDFKKNNGYRRSVEMCEEYDIYRQCYCGCVYAAKM
                     QGIDLVQVKKDAKAFMADKDLDNDFTHIRFSYRGDEM"
     gene            complement(6343..8127)
                     /locus_tag="VHD02_001544"
     CDS             complement(6343..8127)
                     /locus_tag="VHD02_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5498139.1"
                     /translation="MKTARFFWFYFKRYRFSFTVIAVAVILATYLQVKAPVFLGESLT
                     ELGKIGQAYYVAKMSGQTHFSPDLSAFNAVMFKLLMTYFFTVLANLIYSFLLTRVVSH
                     STNRMRKGLFGKLERLTVAFFDRHKDGEILSRFTSDLDNIQNSLNQSLVQVVTNIALY
                     IGLVWMMFRQDSRLALLTIASTPVALIFLVINIRLARKYTNIQQQEVSALNAFMDETI
                     SGQKAIIVQGVQEDTMTAFLKHNERVRQATFKRRLFSGQLFPVMNGMSLINTAIVIFV
                     GSTIVLSDKSMPAAAALGLVVTFVQYSQQYYQPMMQIASSWGELQLAFTGAHRIQEMF
                     DETEEVRPQNAPAFTSLKEAVAINHVDFGYLPGQKVLSDVSIVAPKGKMIAVVGPTGS
                     GKTTIMNLINRFYDVDAGSITFDGRDIRDYDLDSLRQKVGIVLQESVLFSGTITDNIR
                     FGDQTISQDMVETAARATHIHDFIMSLPKGYNTYVSDDDNVFSTGQKQLISIARTLLT
                     DPEVLILDEATSNVDTVTESKIQRAMEAIVAGRTSFVIAHRLKTILNADHIIVLKDGK
                     VIEQGNHHELLHQKGFYAELYHNQFVFE"
     gene            complement(8130..9836)
                     /locus_tag="VHD02_001545"
     CDS             complement(8130..9836)
                     /locus_tag="VHD02_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5498140.1"
                     /translation="MLKDAILRYKWYALGSFFMVIGVVASSLLQPHYLKSVLSAVIQN
                     NQEKIYEVGKPLLIIALLGLMSGAINTVLAAKIAQGVSADMREKTFRKIQDFSYANIE
                     AFNAGNLVVRLTNDINQIQSLVMMMFQILFRLPILFIGAFIMAVQTFPQLWWVIVVMV
                     ILIALIMGLVMRQMGPRFGKFQRLMDKINRIAKENLRGVRVVKSFVQEQQQYTKFKET
                     SNDLLALNLSIGYGFSLMQPALMLVSYLAVYVSINVVSTMVETDPTVIGNIASFMTYM
                     MQIMFSIIVVGSMGMQVSRAFVSMARIRQILSTEPAMTFENEKEETISGSIVFDDVSF
                     TYPNDDEPTLKHISFAIEPGQMVGIVGATGSGKSTLVQLIPRLFDPQDGQILLGGKPI
                     KTLSQTTLRQSVSIVLQKAILFSGTIADNLRQGSAKADIDAMQKAAQIAQAKEFIDRM
                     DSRYESQVEERGSNLSGGQKQRLSIARGVINHPKILILDDSTSALDAKSEKRVQEALS
                     HKLEGTTTVIVAQKISSVVKADKILVLDQGQLIGEGTHAELVANNAIYREIYETQKGR
                     EE"
     gene            complement(9829..10278)
                     /locus_tag="VHD02_001546"
     CDS             complement(9829..10278)
                     /locus_tag="VHD02_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEQ5498141.1"
                     /translation="MSQVIGDLRELIHQIEQISDEIAKKYDVEHLAGPQGYVLVFLAK
                     HQNQEIFVKDIEKQLRISKSVASHLVKRMVKNGFINVMPSQVDKRYKQVVLAQVGRDK
                     LPLLRECRKDIEHYFLKEITKEELLTAKKVIEQLKQNMLTYKGDNDA"
     gene            10576..11592
                     /locus_tag="VHD02_001547"
     CDS             10576..11592
                     /locus_tag="VHD02_001547"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEQ5498142.1"
                     /translation="MTKQKVAILGPGSWGTALSQVLNDNGHDVRLWGNIPDQIEEINT
                     KHTNRHYFKDIVLDKNITATLDLGQALSDVDAVLFVVPTKVTRLVARQVAAILDHKVV
                     VMHASKGLEPETHERLSTILEEEIPAHFRSEVVVVSGPSHAEETIVRDITLITAASKD
                     IEAAKYVQSLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGYGDNAKAAVI
                     TRGLAEITRLGVKLGADPLTYSGLSGVGDLIVTGTSVHSRNWRAGAALGRGEKLEDIE
                     RNMGMVIEGIATTKVAYEIAQDLGVYMPITTAIYKSIYEGADIKESILGMMSNEFRSE
                     NEWH"
     gene            11625..12524
                     /gene="galU"
                     /locus_tag="VHD02_001548"
     CDS             11625..12524
                     /gene="galU"
                     /locus_tag="VHD02_001548"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEQ5498143.1"
                     /translation="MTKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALKSGIEEILIVTGKSKRSIEDHFDSNFELEYNLQAKGKIELLKLVDETTSINLHFIR
                     QSHPRGLGDAVLQAKTFVGNEPFVVMLGDDLMDITNPNVKPLTKQLIDDYEETHAATI
                     AVMRVPHEDVSNYGIIAPQAKAVKGLYSVDTFVEKPQPQDAPSDLAIIGRYLLTPEIF
                     SILEKQEPGAGNEVQLTDAIDTLNKTQRVFAREFKGKRYDVGDKFGFMKTSLDYALKH
                     PQVKDDLKAYIIQLGKALEKTKP"
     gene            complement(12623..13294)
                     /locus_tag="VHD02_001549"
     CDS             complement(12623..13294)
                     /locus_tag="VHD02_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEQ5498144.1"
                     /translation="MTQLLKRYPITIFLLGLTGLIFIAMQVVYGHLATGAQAIYQVGG
                     MFGLLVKAMPDQLWRLVTPIFIHIGFGHFFVNGLTLYFVGQIVEDLWGSRRFLLLYVL
                     SGVMGNAFTFWLTPETVAAGASTSLFGLFAAIVVLSFLGKNQALKDLGKSYQTLIVVN
                     LLMNLFMPNVSMAGHIGGVVGGALLSIVFPTKMRVITVKKTKRMLALVSYGIILVGVL
                     VLGFL"
BASE COUNT         4156 a         2989 c         2459 g         4038 t
ORIGIN      
        1 actaaactag tctctttcag catataccaa aaaaatattg tacactaatt gattcataaa
       61 aaccatttct ttaaatcaca ctaatcaaaa cctcaccgtt tgctgtcatt atcacgtaag
      121 catctcacca cctctctttc aaatccaatc tccaaaatag aaataaacta tactattttt
      181 tactttacca aggtagcaag ctcagataac gataaaaaag actgaagaac ttaatcttca
      241 atctttcgtt gtcgtgactt tttaactcct taaagcgttt aaaaataaag gtgaacgata
      301 cagccttata gcatgttttt gatgtgctca atggatttat cacgtcccaa taaataaatc
      361 gtatttggta attctgggcc atgcatttca ccagatactg caatacgaat aggcataaag
      421 aggttttttc ctttgattcc tgtttctttt tgaacagcct taatttgtgg gaaaatattt
      481 tctggtttga agtcttcatc tgacatagct tccaacttag ccttgaaagc ttgcaagact
      541 gtcgaaactg tttcacctgc catcacttct ttttcggctt ctgttaattc agggaaatca
      601 gaaaagaaaa ggtcagtcaa tggaataatc tcatctgcag attttaattg aggtttgtat
      661 aattctacta gcttctcagc tttttctgtc aaacggcctg cctcttcaag gaaaggtttg
      721 cagagggcat aaactgtttc aaaatccgca tgtttaagat actcattgct catccaatcc
      781 attttcttct ggtcaaaggc agctggcgac ttgctaaggc gattttcatc aaaaagggca
      841 atcagttgct cacgagagaa gatttcctct tctccgccag gattccagcc aagaagggcg
      901 atgaagttaa agacagcttc aggcatataa ccttttttgc ggtaatcttc aatgaattgg
      961 agggtattgg tgtcgcgttt tgataatttt ttacccgttt cagaattgat gataagagtc
     1021 atatgaccaa actctggcgc ttcccagcca agagcttcat agaccatcaa ttgttttggc
     1081 gtattggcaa tatggtcatc gccacggata acgtgagaaa tttgcatatc atgatcatca
     1141 acaacaacgg caaagttata agttgggtac ccatctttct tttgaataac ccagtcacca
     1201 ccgatgttac caccttcaaa ctcgatatcg cctttaacca tgtcagtcca tttgtaaata
     1261 ccggactcat taacagcaag acggactgtt ggaacgatac ctgctgcttc acgctcagca
     1321 atgtacttag ctttttcgtc agctgacata ccaataaatt cattgatata gcgaggcgtt
     1381 tcgccagctg cttcttggcg ttcacgctct gctgccaatt cttcctcagt cacgtaagac
     1441 ttataggctt ttccttcagc taacaattga tcaatatatt gttgataaag tgctagacgt
     1501 tcagactggc ggtaattttc atgcgtttct gggctttcat cccaatccat tcctaaccat
     1561 tttaggtttt caagttgaga acgttcacca tcttcaacat gacgtttacg gtcagtatct
     1621 tcaatacgaa tgataaaagt acctccgtga cgacgggcat aaaggtaatt aaatagggct
     1681 gtgcgggcat tcccgatatg taaaagccct gttggacttg gtgcataacg cacacgaatt
     1741 ggtttagaca tgcttcttct cctgttacat atatcactca gaaacctgtg gcacgaaaaa
     1801 caagcaacaa atgatgattt gagcttgcca agaccttttc agacttcttc taaaatcttt
     1861 cttattatag catattttac ttgcttaggc gaaaaaaatc agccctaaaa agctgatttt
     1921 atgttatttt gactaatttg aactagttgg tagataacgt ttaagagtcc cagcaaattg
     1981 ctcaaggtta agactttgga tataaatctc accatgacct ctaaaggtat taacaactcc
     2041 ttcacctgta ccaattgact gcataaagcc attttctaaa tgaatatcat aatcaagttc
     2101 tctgctccat gctacaacat gggcattatc aatagtcatt gtgccaccat caagtgtgat
     2161 tttcttgata gatccaaacg agttagcaag taaggtaccc aagccttcag ttgtcatgac
     2221 aaaaagtccg ccttgaccac caaaaagtgc ttttccaata ttttgacgtt ccattttgta
     2281 ctgtgctgaa ccatctaggg ctagaaaggc accatcattt agacgatatt gtttttcacc
     2341 tagttctaaa gcaataatct gtcctggtgt attgggtgct aatgctaatt taccatctcc
     2401 atttgacata gcttgtgtaa tgaacattga ttcaccagaa accatggaac ggccgatggc
     2461 tccaactaat tttcctaaac cagatccctt accattcaat ttggtattca aggttacatt
     2521 ctcagtatga taaaccatac ttccttgttg taagtaaact gatcccccat gtcccagatc
     2581 aatctcaact agtggaaatt gcatgttttg atcaatcgta aatctcattc tattgtcgac
     2641 catcttactc tccttgtttg tatgaataac ttgatacaat tattataacg cttccctatt
     2701 tctttgtcta atattttcaa taatttttaa aaataaaaaa gagctgaatt cagctctaca
     2761 acttggctac taactattcc attacttgtg tcatctttcc agtatccaca tcgtagacgg
     2821 caccgttaat aacgacatca tcagagataa gagaggaggc tcttatctta gtcatatcct
     2881 cgcggacact gtcttccaca tcttggaagg gaaggaaatc ttgccctgaa acatccatac
     2941 ctaagtgctc atgaatatgc ttggcaaagc cttcattagt aaaagtctgc gcgccacaat
     3001 ctgtatggtg gagaaccaca atctctcgcg ttcccatttg ctgctgagaa ataaccagcg
     3061 accgaatcat gtcttcggtg acacgtccgc cagcattcct taaaatatga gcatctccta
     3121 gagccagccc cagagcttgg gccacatgta gccgtgaatc catacaagtc acaatcgcta
     3181 ctttagtctt gggttttaag ggcaaatgag ctgtcccatg caaggccaca taagcctgat
     3241 tggcagccat aaaatgttca aaataagaca tcaataaact ccttttgaga aacagcagag
     3301 ctatttttgc tgttagtttg tcttatctta acatctttta tggaaaatag ctggattaac
     3361 ctcttagacg gtcggtatcg ttcgaaaagt taaaaaaaca aatgattggg atactttctt
     3421 gacaaatttc ctcctcattt gctcttagtt tatgatcctc tagctaaaaa ctgcctttaa
     3481 aacctgacca accgtagtca ctccgacaac ctcaatacct tgagggatat caatgccttg
     3541 caaggcattt ttaggagcat agaccttggt aaatccaagc tttgctgctt cattaatccg
     3601 ttgctcaata cgggtcactc gccgaatctc acctgttaac ccgatctcgc ctaagaaagc
     3661 ttcttgaggg ctggttggtt tttccttata actggatgcg atagccacag cgactgccaa
     3721 atcaatggca ggttcgtcta gtttgacacc accagccgac ttgaggtagg catcttgatt
     3781 ttgtaagagt aaaccacagc gcttttctaa aactgccatt attaggctta cgcgattgaa
     3841 atcaagtccc gtagtggttc gtctggcatt gccaaaaacg gttggtgtca ctaaagattg
     3901 cacctctgct aagataggac gacttccttc catcgttaca acgacagccg atcctgttgc
     3961 cccatctaag cgttcctcta aaaagacttg actaggattt aagacctcta ccaagccgcc
     4021 agactgcatt tcaaagatgc caatctcgtt agtggagcca aagcggtttt tcacagcccg
     4081 aagaatgcga aaggtgtgat gcctttcccc ttcaaagtag agaacggtat ccaccatgtg
     4141 ttctaacata cgaggcccag ccaaggttcc ttccttggtg acatgaccca caataaaggt
     4201 tgcaatgttg ttcgtctttg caagctgcat caattctgca gtgacttctc tcacctgact
     4261 gactgatcct tgaacacctg taatatcagg gctcatgatg gtctgaatgg agtcaataat
     4321 taaaaaatct ggtttaatat tttcaatttc tgtgcgaata gcttgcatat tggtttcggc
     4381 atagaggtaa aattcattgt caatatcacc cagacgctca ctccgcagtt taatctgctc
     4441 tgctgattct tccccagacg catagagaac cgttccctta ttagcgagct gcgtagatac
     4501 ctgcaagagt aaggtggatt ttccaatacc tggatcacca ccaattaaaa tcaagcttcc
     4561 aggaaccaca ccccctccaa gaactctatt aaattcagac atgtcagtct gtgttctgtg
     4621 ataactaata ttatccacat cttttagttt aaccggtctg gacttttctc cagctagact
     4681 aacacgggca tttttgactt ctttgacctc tacttcttcc acaaaagaag accaggccga
     4741 gcaatttgga cagcgcccta aatactttgg cgactggtag ccacactcct gacagataaa
     4801 ggttgctttt ttctttgcca tagtaatctc cctcccctag acaagacaaa cccactctta
     4861 aagaataggt ctgctgtcat aagcattttt gttaaatcgc tttactgccc tgtcgaacca
     4921 aaaccacctg ttcgttcacc tctggcttgg tcaccatcag caattaaaaa aggcataaaa
     4981 accccctgaa cgatcctctc acccacagct aatgtcacag ggtgatctgt aatgttttgc
     5041 atttgtgcaa aaatatgtcc ttcattagcc tcatttccat aataatctgc atcaatcaca
     5101 cctactgaat taatcaggat aatccctttt tttcgtggat ttgacgaacg atcatagagg
     5161 taaagcacct caccatcttg catataggcc ttaacccctg ttggtaccaa cttaatctcc
     5221 cctggggcaa tggttacagc ctcagcaaca gacaggtcat aaccagctgc atgagtcgtc
     5281 tcacgttttg gtagtaaatc aggattagta aagcttgaaa caagctcgaa accacgaatt
     5341 tttgtcatta tgataggtat atcctttcaa gtcaatcata tcctcttatt atagactatt
     5401 cgtaaaagaa tagcaaaaaa gcggtcaact ttaaacggac cacttttaat catgccatca
     5461 catctcatcc ccacgataac taaagcggat gtgggtgaaa tcattatcca aatccttgtc
     5521 tgccataaaa gctttggcat cttttttcac ttgaaccaag tcaatacctt gcattttggc
     5581 agcatagaca cagccacaat agcattgacg gtaaatatca tactcctcac acatttcaac
     5641 ggatctgcga taaccgttgt ttttcttgaa atcacttggc aggtatttgg tggtgtaaac
     5701 cttttgcaca tcgatgccca catcattaat ggtttgcgaa tttttatgag gactgatggt
     5761 tagggcactg gcaaagtagt caaaacctag ttccactgct ttttgagcgg tcttgtctag
     5821 tcggtaatca aaacagacac ggcaacggtc cccaccttct ggttcctctt ctaagcctcg
     5881 cacttgtcga acgtactcat tggggacata gtccgcttcg agaaactgaa cagtattgcc
     5941 tgttttagcg ttgaactcgg agacaaactg ttgtgtcaca taggctctgc gatggtattc
     6001 gtccttaggg tgaatattag aattggcaaa gtaaaccgta atgtcagcaa actgcgtcag
     6061 gtattctagg gtgtaagtgg agcatggggc gcagcagaca tgcattaaaa tactgggacg
     6121 cacggattcc ttctcccaga ccttggccat ctgttgcatg accctgtcat aatttatctt
     6181 ttgattggga ttcatgttag cgagaatctc ttgtaaatca atcattgtgg ctactttcta
     6241 attgtttttt ctcttgcttg ctattatacc atatcaggaa agaaagcttg actgctttat
     6301 tgttcaccaa aaaactccgc taagacgcgg agctgatacc atctattcaa agacaaattg
     6361 attgtgatac aattcggcat aaaagccttt ttgatgcaat agctcatgat gatttccttg
     6421 ctcaatgacc ttgccatctt tcaacacaat aatgtgatct gcatttaaaa tggttttgag
     6481 gcggtgagca atgacaaagc tagttcgacc tgccacgata gcttccatgg cccgttgaat
     6541 tttactttcg gtaaccgtat caacatttga agtggcctca tccaaaatca acacttcagg
     6601 gtcagtcagt agcgtcctag caatagaaat caactgcttt tgacctgttg aaaagacatt
     6661 gtcatcatct gagacatagg tattgtaccc ttttggtaag gacatgataa agtcatgaat
     6721 atgggtcgca cgcgcagcag tttcaaccat gtcttgacta atggtctgat caccaaaacg
     6781 aatattatcc gtaatggttc ctgaaaaaag aactgactct tgcaacacaa tccctacctt
     6841 ttgacgaaga ctatccaaat cgtagtcacg aatatcacgg ccatcaaagg taatcgaacc
     6901 tgcatccaca tcgtagaaac ggttaatcaa gttcataata gtggtctttc cagaacctgt
     6961 cggtccaacc acggcaatca ttttgccctt gggtgcaacg attgacacat ctgataaaac
     7021 tttttgccca ggaagatacc caaaatcgac gtggttaatc gccactgctt cttttaagct
     7081 ggtgaacgct ggtgcatttt gtggacgaac ttcttcggtt tcatcaaaca tttcttgaat
     7141 acggtgagca ccggtaaagg ccagctgcaa ttctccccaa ctagacgcga tttgcatcat
     7201 gggttggtaa tattgctggg aatattgtac aaaagtaacc actaaaccaa gcgctgccgc
     7261 tgctggcata gatttgtcac tgaggacaat tgttgaaccg acaaaaatca cgatagccgt
     7321 gttaataagg ctcattccat tcatgactgg aaataattgt cctgagaaca gacggcgttt
     7381 gaaggtggct tgtcgaaccc tttcattatg ctttaaaaag gctgtcatcg tatcttcttg
     7441 gacaccttgt acaataatag ccttttgtcc tgaaatggtt tcatccataa aagcatttaa
     7501 agcactgact tcttgctgtt ggatattggt gtattttctt gccaaacgga tgttaatcac
     7561 taaaaaaatg agagcaactg gggttgatgc gatggttaac aaagctaaac ggctatcttg
     7621 cctaaacatc atccagacca ggccgatgta aagggcaata ttagtcacca cttgaaccaa
     7681 ggattggttc agcgagtttt gaatattatc caaatcactc gtgaaacgag aaagaatctc
     7741 cccatcttta tggcggtcaa aaaaggcgac ggttaaacgt tctaatttac caaacaagcc
     7801 cttgcgcatg cggttggtcg aatgtgagac aacacgtgta agtaagaaac tatatattag
     7861 attagctaaa acagtaaaga aataagtcat caaaagctta aacatcacgg cattaaaagc
     7921 tgataaatca gggctaaaat gtgtctgacc actcatctta gcaacgtaat aagcctgacc
     7981 gatttttccc aactcagtca aggactcccc taagaagaca ggagctttta cttgtaaata
     8041 agttgctaag ataacggcaa cagcaatgac agtaaacgag aaacgatagc gtttaaaata
     8101 aaaccagaaa aaacgtgctg ttttcatcac tactcctccc ttcctttttg tgtctcataa
     8161 atttcacgat aaatcgcatt attagccacc aactctgcgt gtgttccttc tccaattaac
     8221 tgtccttggt caaggactaa gatcttatct gccttaacta ccgatgaaat tttttgagca
     8281 acaataactg tagttgtacc ttctaattta tgacttaagg cttcttgaac gcgtttttca
     8341 gattttgcat caagagctga agtggaatcg tctaatataa ggattttggg atgattaatg
     8401 acaccacgtg caatagacag acgttgcttt tgtccgccag acaagttact acctcgctct
     8461 tcaacttggc tctcataacg gctatccatg cgatcaataa attcttttgc ttgcgcgatt
     8521 tgagcggctt tttgcatagc atcaatatca gcttttgctg agccttggcg aaggttatca
     8581 gctatcgttc ctgaaaagag aatagccttt tgcaacacga tggaaaccga ttggcgcagg
     8641 gttgtttgac ttagggtttt aatcggcttt cctcctaaca aaatttggcc atcttgagga
     8701 tcaaataagc gagggatgag ctgtacaagc gttgatttcc cagatcctgt tgcaccaacg
     8761 atgccgacca tttgcccagg ttcaatagca aaagaaatgt gcttcaaggt tggctcatca
     8821 tcattaggat atgtaaaact aacatcgtca aatacaatgc ttcctgagat ggtttcttcc
     8881 ttttcatttt caaatgtcat ggcaggttct gtagacaata tttgcctaat tctagccata
     8941 gaaacaaaag cacgactaac ttgcatcccc atggaaccaa ccacaataat tgaaaacata
     9001 atttgcatca tataagtcat gaaagaggca atattcccaa tcactgttgg atctgtttcg
     9061 accatggtcg aaacaacatt tatagaaaca taaactgcca aataagacac caacattaaa
     9121 gccggctgca ttagtgaaaa accataacca atagataaat ttaacgccaa aagatcattg
     9181 gacgtttctt taaatttagt gtattgttgt tgttcttgta caaatgattt aaccacgcga
     9241 acgccacgga gattttcctt agcgatgcga ttaatcttat ccattaaccg ttgaaattta
     9301 ccaaaacgag gacccatttg acgcatcacc aaacccatga tgagggcaat taagataacc
     9361 attacaacaa tgacccacca taattgaggg aatgtctgaa ctgccatgat aaacgcgcca
     9421 ataaaaagga tcggcaaacg aaagagaatt tgaaacatca tcatgactaa actttggatt
     9481 tgattgatat cattagttag cctaaccaca agattaccag cattaaaagc ttcaatattg
     9541 gcatatgaaa agtcttggat ttttcgaaag gtcttctccc tcatatcagc agatacacct
     9601 tgagctattt tagcagctaa aaccgtatta atcgctccag acattaaccc taaaagggca
     9661 ataataagta atggtttccc aacctcataa attttctctt gattattttg aataacagcg
     9721 cttagcacag atttaagata atgtggttgc aagagactac ttgcaacaac cccaataacc
     9781 ataaaaaatg agcctaaagc ataccattta taacgtaaga ttgcgtcttt aagcatcgtt
     9841 gtctccttta taagttagca tattttgctt gagctgttca attacttttt tcgctgtcag
     9901 caactcttct tttgtaattt cttttaaaaa atagtgctcg atatccttac gacactcccg
     9961 caacaaaggc aatttatctc taccaacctg cgctaacact acttgcttat aacgcttatc
    10021 cacttgggaa ggcatcacat tgataaaccc atttttgacc atacgtttca ctaaatgact
    10081 agcaactgac tttgagatac gaagttgttt ttcaatatct ttgacaaata tttcttgatt
    10141 ttggtgttta gctaaaaaaa caagaacata accttgagga cctgctagat gctctacatc
    10201 atattttttt gcaatctcat cactaatttg ttcgatttga tgtatcaatt cacgtaaatc
    10261 acctatcact tgtgacatct cttccctcct tcttttcctt aatagtttct atgataagta
    10321 ttttagttct ctagataact attgtcaatt aaaatatctt tatttttaaa aataagtttc
    10381 cgaacttaaa taactaaaaa gaacaaggca aagccatgac aacagctcac ctgatgaagc
    10441 ttattaaaca acattattaa tgtagctagc tctcttgaga acgccttatt agccaagacc
    10501 atataccttt tctaatagag ctaatcaagg tgttatgtta taatagagtg actagctctt
    10561 gaaggagaat ttagaatgac aaaacaaaaa gtagctattt taggacctgg ttcttgggga
    10621 actgctcttt ctcaggttct taatgacaat ggccatgacg tccgtttatg gggaaatatt
    10681 cctgatcaaa ttgaagagat aaataccaag cataccaata gacactactt caaagacatt
    10741 gtgttagaca aaaacattac ggcaactctt gatttaggtc aagccctatc agatgttgat
    10801 gcggtgcttt ttgttgtgcc aactaaagta acacgattag ttgctagaca agttgccgcc
    10861 attttagatc ataaagtagt ggtcatgcat gcctcaaaag gtctagaacc agagacccac
    10921 gaacgtttat caactatcct agaagaagaa attccagctc attttcgtag tgaagtggtc
    10981 gttgtatcag gtcctagtca tgctgaagaa actatcgttc gcgatattac tctcatcacg
    11041 gcagcctcaa aagatattga agctgcaaag tatgttcagt ccctctttag taaccactac
    11101 ttccgacttt ataccaatac cgacgtcatc ggagttgaaa cagctggtgc cctcaaaaac
    11161 attattgctg ttggagcagg agctctccat ggacttgggt atggagataa tgccaaagca
    11221 gcggtaatta ctcgtgggtt agctgaaatc acccgtctag gcgtcaagct tggtgctgac
    11281 ccgttaacct acagtgggtt atctggtgtt ggtgatctaa ttgtaacagg tacttctgtc
    11341 cattcacgta actggcgggc tggagctgcc cttggacgtg gtgaaaaatt agaagatatt
    11401 gaacgcaaca tgggtatggt tatcgaaggc atcgcaacaa ctaaggtagc ctatgaaatt
    11461 gcccaagacc tcggagttta catgccaatt acaacagcta tttataagtc catctacgag
    11521 ggcgctgata tcaaagaaag catcctcggc atgatgtcaa atgaatttcg ttctgaaaat
    11581 gaatggcatt aagccatacg tcactaaaat aagaggaggt gctgatgacc aaagtcagaa
    11641 aagctattat tcccgcagca ggtttaggca cacgtttttt acctgcaacc aaggcccttg
    11701 ccaaggagat gctacctatt gttgataaac caactattca atttatcgtt gaagaagctc
    11761 taaaatcagg tatcgaagaa attcttatcg ttactggaaa atccaaacgc tccatagaag
    11821 atcattttga ttctaatttt gaattagaat ataatttaca ggctaaagga aaaatcgaac
    11881 ttctaaagtt agtagacgag accacttcta ttaaccttca ctttattcga cagagtcatc
    11941 caagaggatt aggagacgca gttcttcaag caaagacctt tgttggcaat gaaccttttg
    12001 ttgtcatgct aggtgatgat ctgatggata ttacaaatcc aaatgtaaaa ccattaacca
    12061 aacaattgat cgatgactat gaagaaactc atgctgcaac cattgccgtc atgagggttc
    12121 ctcacgagga tgtctctaac tacggtatca ttgctcctca agcaaaagca gtaaaaggac
    12181 tctatagtgt ggatactttt gttgaaaaac cacaacctca agatgcacct agtgatttag
    12241 caattattgg acgttacttg ctcacaccag aaatttttag tattttagaa aaacaagaac
    12301 ctggtgcagg caatgaagtt caactgactg atgctattga taccctcaat aagacacagc
    12361 gtgtctttgc tcgtgagttt aaagggaaac gctatgatgt tggcgataaa tttgggttta
    12421 tgaaaacttc tttggattat gccctgaaac accctcaagt caaagatgac ttgaaagcct
    12481 acatcatcca actgggcaaa gcactagaga agacaaaacc ataaaaataa ggagggaaaa
    12541 tccctcctta tttttatggt ttttaagtca tttcatccta ctagccatct gagaagtaac
    12601 agtgctaacc actcacttat ttttataaaa acccaagcac taacacacca accaaaataa
    12661 taccatagct cactaaagcc aacattcgct tggttttctt cactgttatc actctcatct
    12721 tagttggaaa aactatactc agtaacgcac cacccacgac accgccaata tggcctgcca
    12781 tactcacgtt tggcataaaa agattcatca gtaaattgac tacaattaac gtctggtatg
    12841 acttgcccaa atctttcaac gcttggttct tacccaaaaa actcaatact acaatagcag
    12901 caaaaagtcc aaataacgac gtcgatgctc ctgcagctac tgtttcagga gtcaaccaaa
    12961 aagtaaaggc attgcccata acacctgata aaacataaag taataaaaaa cggcgtgagc
    13021 cccagaggtc ttcgacgatt tgaccaacaa aatatagtgt taatccattg acaaaaaaat
    13081 gaccaaagcc gatatgaata aatatcggag tcaccaatcg ccacaactgg tctggcattg
    13141 cttttactag taatccaaac atacctccaa cttgataaat agcttgagca ccagttgcta
    13201 aatgtccata taccacttgc attgctataa aaattaagcc tgttaaccct aaaaggaaaa
    13261 tagtaatagg atatcgtttt aataactgtg tcatctaaga taaacctcct aaacagcaat
    13321 atcataaatc gtttgatttg gcaatcgtat ttgcccgctg ataagcttga ttgctaacaa
    13381 catccactaa taaagcttga tcattttgac gttttaagtc tacatccata gttcgtaaag
    13441 actagaagag ttctttgaga atctcttttt tcatcatctt cttatcatac cataaattag
    13501 gaaaaaggtt caaggctgac aagaaactag ccacgccgta cgcatgaaaa ctttctcttc
    13561 atccttttgt gcccggacat caattttgaa aagattcctt tttaaatcaa tcatctgctt
    13621 agcctctttc tccaaataat tg
//
LOCUS       DAUDYX010000051        13354 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.052, whole genome shotgun sequence.
ACCESSION   DAUDYX010000051 DAUDYX010000000
VERSION     DAUDYX010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13354
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.052"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(109..2016)
                     /locus_tag="VHD02_001550"
     CDS             complement(109..2016)
                     /locus_tag="VHD02_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEQ5498145.1"
                     /translation="MIILQGNKLERSFSGDVLFQNISLQVDERDRIALVGPNGAGKST
                     LLKLLVGEETPTSGEVNTKKDLTLSYLAQNSRFESDKTIYEEMLKVFEALRQDEKRLR
                     QMEMDMATVSGQDLTRLMTDYDLLTEHFRQQGGFTYESDIKAILNGFKFDESMWQMTI
                     AELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIETIAWLENYLANYQGALIIVSHD
                     RYFLDKVATVTLDLTPHGLDRYVGNYSRFMTLKAEKLVAEEKQFDKQQKEIAKLEDFV
                     QKNIVRASTTKRAQARRKQLEKIERLDKPTSARKSAHMTFQADKPSGNVVLTVEKAAI
                     GYNQHVLSEPINLDVHKLDAIAIVGPNGIGKSTLIKSVIGQIPFIKGEVKYGANVEIG
                     YYDQTQSHLTSSNTVLEELWQDFSTTPEVDIRNRLGAFLFSGDDVKKSVAMLSGGEKA
                     RLLLAKLSMENNNFLVLDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRLAT
                     KVLEITENGSTLYLGDYDYYLEKKAELEELARLAAGETVEETKEASATDYQLQKANQK
                     ERRRLTRRYEEIEARLETIEERIGAIQEDMHASNDTAQLIAWQKEWDQLDQEQETLME
                     EWETIAEQIES"
     gene            complement(2062..2388)
                     /locus_tag="VHD02_001551"
     CDS             complement(2062..2388)
                     /locus_tag="VHD02_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498146.1"
                     /translation="MKQLLAIILWLPKLIVKMFWHLIKGFLQTILLVTIIIIGLMYYS
                     NHSDSVLANKISIVTEQVVQIFDILTQKPSAKTRHGSGNSHSTFLVSAKEGNQRLKKS
                     SISFGN"
     gene            complement(2418..3203)
                     /locus_tag="VHD02_001552"
     CDS             complement(2418..3203)
                     /locus_tag="VHD02_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEQ5498147.1"
                     /translation="MASIAIEYHSVVLGMERKVNVIYPDQSEIPKKDQGDKDIPVLYL
                     LHGMGGNENSWQKRTAIERLLRHTNLIVVMPSTDLGWYTDTAYGLNYYRALSQELPQV
                     LAAFFPNMTQKREKTFVAGLSMGGYGAFKWALKSNRFSYAASFSGALDFSPETLLEGK
                     LGELAYWQGVFGQFDDPDLDKHYLKNMVAESDGKTKFYAWCGYEDFLFATNEKAIADF
                     QAQGLDIDYHKGHGKHEWYYWNQQLEVLLEWLPINYQKEERLS"
     gene            complement(3336..4997)
                     /locus_tag="VHD02_001553"
     CDS             complement(3336..4997)
                     /locus_tag="VHD02_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEQ5498148.1"
                     /translation="MTDIKMIALGGVREYGKNFYLVEINDSMFILDAGLKYPENEQLG
                     VDLVIPNLDYVIENKGKVQGIFLSHGHADAIGALPYLLAEVSAPVFGSELTIELAKLF
                     VKSNNSTKKFNNFHVVDSDTEIEFKDGLVSFFRTTHSIPESMGIVIGTDKGNIVYTGD
                     FKFDQAAREGYQTDLLRLAEIGKEGVLALLSDSVNATSNDQIASESEVGEEMDSVISD
                     ADGRVIVAAVASNLVRIQQVFDSATAHGRRVVLTGTDAENIVRTALRLEKLMITDERL
                     LIKPKDMSKFEDHELIILEAGRMGEPINSLQKMAAGRHRYVQIKEGDLVYIVTTPSTA
                     KEAMVARVENLIYKAGGSVKLITQNLRVSGHANGRDLQLLMNLLKPQYLFPVQGEYRD
                     LAAHAKLAEEVGIFPENIHILKRGDIMVLNDEGFLHEGGVPASDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVAITVSKKEKRIISKAKVNTRGFVYVKKSHDILRESAELVNTT
                     VGNYLKKDTFDWGELKGNVRDDLSKFLFEQTKRRPAILPVVMEVR"
     gene            complement(5201..5959)
                     /locus_tag="VHD02_001554"
     CDS             complement(5201..5959)
                     /locus_tag="VHD02_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5498149.1"
                     /translation="MTKIIELINATVDVDNGFEDAKTILDNVTLTIYEHDFLTILGGN
                     GAGKSTLFNVIAGTLSLTRGQIRILGQDVTHWPAEKRALYLSRVFQDPKMGTAPRMTV
                     AENLLIARQRGEKRSLASRKITEHLASFEDLVKRTGNGLEKHLETPAGLLSGGQRQAL
                     SLLMATLKKPALLLLDEHTAALDPKTSQSLMQLTDEFVTKDGLTALMITHHMEDALTY
                     GNRLIVMKDGNIIKDLNQMEKEQLTITDYYQLFD"
     gene            complement(5956..6825)
                     /locus_tag="VHD02_001555"
     CDS             complement(5956..6825)
                     /locus_tag="VHD02_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ5498150.1"
                     /translation="MIISSVSQGLIWGVLGLGIYLTFRILNFPDMTTEGSFPLGGAVA
                     VTAISLGWNPFLSTLLGMLSGALAGFLTGLLYTKGKMPTLLAGILVMTSCNSIMLMVM
                     GRANLGLHDHKRIQDCLPFSIDLNSLLTGLITVVIVISVLIYFLYTNLGQAYIATGDN
                     KDMAKSFGINTDWMEVMGLVVSNSLIALSGALVSQQDGYADVSKGIGVIVIGLASIIV
                     GEVLYSTGLTLLERLIAIVIGSILYQFLISVVITLGFNTSYLKLISALVLALCLMIPV
                     VKERYFKGVRLTR"
     gene            complement(7170..8168)
                     /locus_tag="VHD02_001556"
     CDS             complement(7170..8168)
                     /locus_tag="VHD02_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEQ5498151.1"
                     /translation="MKNKSLIATLLVLTVIVIGSLLSKGVSKENRDLANRQNITIGIL
                     QFVTHEALDDIKRGIEDQLKKQMPQKQNVVIKVMNAEGDQSKIQTMSRQLVQSGSDIV
                     IGIATPAAQGLAATSKDIPVVMSAVSDPVGSRLVMQLDQPEANVTGLSNKVPVKQTID
                     LMKKLTPHVKTVGILYASNEDNSLSQVKEFRRLARKKGYQVISYAVPSTNEVPATMSV
                     MLGKVDAVFIPQDNTIASAFSSVMTTSKAAKIPVYTSVDRMVEKGGLAAISQNQYDLG
                     VQTANQVLKLIKGKRVVDVPVKVVDIGQPLINKNVAAELGIAINREDFPSASFIEN"
     gene            8522..10174
                     /gene="fbp54"
                     /locus_tag="VHD02_001557"
     CDS             8522..10174
                     /gene="fbp54"
                     /locus_tag="VHD02_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein Fbp54"
                     /protein_id="HEQ5498152.1"
                     /translation="MSFDGFFLHHLTNELKENLLYGRIQKVNQPFERELVLTIRNHRK
                     NYKLLLSAHPVFGRVQITQADFQNPQVPNTFTMIMRKYLQGAVIEQLEQIDNDRIIEI
                     KVSNKNEIGDAIQATLIIEIMGKHSNIILVDRAENKIIESIKHVGFSQNSYRTILPGS
                     TYIEPPKTAAVNPFTITDVPLFEILQTQELTVKSLQQHFQGLGRDTAKELAELLTTDK
                     LKRFREFFARPTQANLTTASFEPVLFSDSHATFETLSEMLDHFYQDKAERDRINQQAS
                     DLIHRVQTELDKNRNKLSKQEAELLATENAELFRQKGELLTTYLSLVPNNQDSVILDN
                     YYTGEKIEIALDKALTPNQNAQRYFKKYQKLKEAVKHLSGLIADTKQSITYFESVDYN
                     LSQASIDDIEDIREELYQAGFLKSRQRDKRHKRKKPEQYLASDGKTILMVGRNNLQNE
                     ELTFKMAKKGELWFHAKDIPGSHVIIKDNLDPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLHKPSGAKPGFVTYTGQKTLRVTPDQAKILSMKLS"
     gene            complement(10233..11480)
                     /locus_tag="VHD02_001558"
     CDS             complement(10233..11480)
                     /locus_tag="VHD02_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEQ5498153.1"
                     /translation="MLNSEKMIASLDQQDLAHAEKYFQKALKEDDADSLIALGEYLES
                     IGFLPHAKRIYLQLADDYPELNINLAQIAAEDDAIEEAFLYLDKVSKDSPNYLSALLV
                     MADLYDMEGLTEVAREKLLQAVSISPEPLVIFGLAEIDMSLQYFKEAIDYYAQLDNRQ
                     ILELTGISTYQRIGRAYASLGKFEAAIEFLEKAVAIEYEDETVFELATLLYDQENYQK
                     ANLYFKQLETINPDYPGYEYGYALSLHEEHKTSEALRLVQQGLRKNAFDSQLLLLASQ
                     LSYELHDRQNAENYLLQAKEVAVDDEEILMRLVTLYFDAERFEEVIALNRETIDNVLT
                     KWTIAKAYHALEQEEVALALYNEISADLAENPEFLQDYAYLLREFGQFHKAIQMATAY
                     LRQVPDDVNMQDFLDHIKHQQSE"
     gene            complement(11470..12651)
                     /locus_tag="VHD02_001559"
     CDS             complement(11470..12651)
                     /locus_tag="VHD02_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEQ5498154.1"
                     /translation="MPLIPEKSRTDSLFYKWFLNNQATMALVITLLAFLTIFVFTKIS
                     FLFMPVISFFAVIMLPLVISTILYYLTKPLVDLINHLGPNRTTSIFIVFGLITLLFVW
                     AISGFVPMVQTQLTSFIEDLPKYVGKVNEEANKLLENEWLVSYKPQLQDMLTHTSQKA
                     LDYAQSFSKNAIDWAGNFAGAIARITVAIIISPFILFYFLRDSSHMKNGLVNVLPLKL
                     RVPMVRVLGDINKQLSGYVQGQVTVAIVVGFMFSIMFSLVGLKYAITFGIIAGFLNMI
                     PYLGSFLAMIPVVIMAMVQGPFMLVKVLVIFMIEQTIEGRFVAPLVLGNKLSIHPITI
                     MFLLLTAGSMFGVWGVFLVIPIYASVKVVIKELFDWYKKVSGLYDEEVLVIEEVKDHV
                     K"
     gene            complement(12709..13185)
                     /locus_tag="VHD02_001560"
     CDS             complement(12709..13185)
                     /locus_tag="VHD02_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEQ5498155.1"
                     /translation="MTQLATICYIDNGDSLLLLHRNKKENDVHKGKWISVGGKLEAGE
                     TPDECARREILEETHLTVTEMAFKGIITFPEFTPGHDWYTYVFKVTGFEGDLISDEES
                     REGTLEWVPYDQVLEKPTWEGDYDIFKWILEDRPFFSAKFTYDQNNQLMDKSVTFY"
BASE COUNT         4136 a         2853 c         2268 g         4097 t
ORIGIN      
        1 taccgacctt gacataaaaa aaacacatca ttttaaaacc tctttcacac cttcaaacaa
       61 gtaacaaagc gataaaaata agcttagcaa aactgctaaa gcttattttt atgattctat
      121 ttgttcagcg atggtttccc attcttccat aagagtttct tgttcttgat ccaattggtc
      181 ccattccttt tgccaagcga ttaactgcgc cgtatcattg gaggcatgca tgtcttcttg
      241 aatagcacca atgcgctctt caatggtttc aagtcttgct tcaatctcct catatcggcg
      301 ggtcaagcga cgacgctctt tttgattagc tttttgtagc tgataatcag tggcagaggc
      361 ttcttttgtt tcctcaactg tctctcctgc tgctaggcgg gctagttctt ctagctctgc
      421 ctttttctca aggtaataat catagtcacc taagtaaagg gttgagccat tttcggtaat
      481 ttccagaacc ttagttgcca aacgattaat gaaatagcgg tcgtggctga caaaaagcaa
      541 ggtgccgtca aagtcaatga gggcattctc tagcacttct ttactgtcaa tgtctaggtg
      601 gttagtcggc tcatccagaa caagaaaatt attgttttcc atagataact tcgcaagaag
      661 cagacgggct ttttctccac ctgacagcat ggccaccgat tttttgacat catctccaga
      721 aaagagaaaa gcccctaatc ggtttctgat atcgacttct ggcgtagttg aaaaatcctg
      781 ccataattct tccaaaactg tgttgctaga cgtcaaatgt gattgggttt ggtcatagta
      841 acctatttca acattggcac cgtatttcac ttctcctttg atgaaaggaa tctgtccaat
      901 aacagattta atcagagtgg atttaccaat accatttgga ccaacaatag caatcgcatc
      961 taatttgtga acgtctaggt taataggctc agataaaaca tgttggttat aaccaatagc
     1021 agctttttca accgtcaaca ccacattgcc agacggctta tctgcctgaa aagtcatgtg
     1081 agctgatttt ctagcactag tcggtttgtc taagcgttct attttttcca attgcttacg
     1141 tctcgcctga gcacgttttg tagttgaagc cctgacaata tttttttgaa caaaatcttc
     1201 taacttggca atttcttttt gctgtttgtc aaactgcttt tcttcggcta ctaatttttc
     1261 agcttttaaa gtcataaaac gagaataatt gccaacgtag cgatccaatc catgaggagt
     1321 taaatccaag gtcactgttg ccaccttatc caaaaaataa cggtcgtgac tcacaatgat
     1381 tagagctcct tgataattgg ccaagtagtt ctctaaccag gcaatggttt caatatctaa
     1441 gtggtttgtt ggctcatcaa ggaccaatag ttctggcttt tctaaaagca ttttagccaa
     1501 ggccagacga gtgttttgac ctcctgacag ctcagcgata gtcatctgcc acatgctctc
     1561 atcaaactta aacccattta aaatggcttt gatgtcagat tcatacgtaa agccgccttg
     1621 ctgacgaaaa tgctctgtaa gcagatcata atctgtcatc agtcgggtta aatcttgacc
     1681 tgaaacggtt gccatatcca tttccatctg acgcaggcgc ttttcatcct gtcgtaatgc
     1741 ttcgaaaacc ttgagcattt cctcatatat cgttttgtca gattcaaaac ggctattttg
     1801 agccagataa gacaaggtta ggtctttttt agtattaact tctccgctcg ttggggtttc
     1861 ttccccaacc aatagcttga gtaaggttga cttaccagct ccatttggcc ccactaatgc
     1921 aattctgtct ctttcatcaa cttgcagtga tatgttttga aaaagaacat ctcctgagaa
     1981 agagcgttct aacttgtttc cttgtaaaat aatcatactt tcattctaac aaaaaaatgc
     2041 ccttttgagg acattttcag cctagtttcc aaaagaaatc gaactctttt taagtcgttg
     2101 attaccttct ttagcgctaa caaggaaagt ggaatgacta ttaccactcc catgacgcgt
     2161 ctttgcagat ggcttttgtg ttaagatatc aaaaatttgc accacttgct cagtaactat
     2221 cgagatctta ttggctaaaa cggaatcact gtgattagaa tagtacatca gccctataat
     2281 aataatcgtt actaataaga tagtttgtaa aaaccctttg ataagatgcc aaaacatttt
     2341 cactattagc ttaggcagcc acaaaatgat cgccagtaat tgtttcattt gtcacctcat
     2401 gttgtactag gaaatggtta agataacctt tcttcttttt ggtagtttat cggtaaccac
     2461 tctaacagaa cttctaattg ttggttccaa tagtaccact catgtttacc atgaccttta
     2521 tgataatcaa tgtcaagacc ttgagcctga aaatcagcaa tagctttttc attagtggca
     2581 aaaagaaaat cctcatagcc acaccaagca taaaatttgg tttttccatc actttctgcc
     2641 accatatttt taaggtaatg cttgtcaagg tcagggtcat caaactggcc aaaaacgccc
     2701 tgccaatagg ctaattctcc cagttttccc tcaagcaagg tctcaggaga aaaatccaaa
     2761 gcacctgaaa acgatgccgc ataagaaaac cgattgcttt tgagtgccca cttaaaggca
     2821 ccataaccac ccatagagag cccagcaaca aaggtttttt ctcgtttctg ggtcatatta
     2881 ggaaagaaag ctgctaagac ctgcggtaac tcttgtgaca acgcccgata atagttaagt
     2941 ccataagccg tgtctgtata ccaacctagg tctgtcgaag gcataacgac tatcaaattg
     3001 gtatgccgca acaagcgttc aatagctgtt cgtttttgcc aagagttttc attgcctccc
     3061 ataccatgta acaaatataa aacaggaata tccttatccc cttgatcttt tttagggatt
     3121 tcagactggt caggataaat cacatttacc ttacgctcca ttcctaaaac taccgaatgg
     3181 tattcaattg caatacttgc cattcattac actcctttat accaaaataa tcctatcacg
     3241 taaatctact gtatttttgg gtgaacagtt tcctggttta ccagttgtta aaatcaaaga
     3301 aggtaggcag tttttgccta accttcctat tttcactaac gaacttccat cacaaccggc
     3361 agaatcgctg ggcgcctttt ggtttgttca aataagaatt tagacaaatc atctctgaca
     3421 ttccctttta attcgcccca gtcaaaggta tcttttttta agtaattgcc aacagttgta
     3481 ttaactaatt ctgcactttc tcttaaaata tcatgacttt tcttgacata cacaaatcca
     3541 cgcgtattaa ctttagcctt agaaataatg cgtttttctt ttttagaaac agtaatagca
     3601 acaatgaaaa taccatcttc tgacaagact ttacggtctc gtaaaacgat attacccaca
     3661 tcaccgatag cattaccgtc aatcatgaca tcactggcag gtacacctcc ttcatggaga
     3721 aaaccttcgt cattaagcac catgatatcg ccacgcttca gaatatgaat attctctgga
     3781 aaaataccaa cttcttcagc tagctttgca tgggcagcta aatcacgata ttctccttgc
     3841 actggaaaaa gatactgggg ttttaacaaa ttcatcaata gttgcaaatc acgcccatta
     3901 gcatggcctg acacgcgtaa gttttgagtg attaatttaa cagaaccacc ggccttgtaa
     3961 atcaagtttt ctacacgcgc caccatagct tctttagccg tacttggtgt agttacaata
     4021 taaacaaggt ctccttcttt gatttgaaca taacggtgac gtcccgctgc cattttttgt
     4081 aagctattaa ttggctcacc catacgacca gcttcaagga tgattaattc atggtcttcg
     4141 aatttagaca tatccttagg tttaattaaa agacgctcat ccgtaatcat taatttttct
     4201 agacgtaaag ctgttctgac aatattttct gcatctgttc cggttaaaac aacacgacgc
     4261 ccatgggcag tggcggaatc aaatacttgc tgaatccgaa caagatttga ggctactgca
     4321 gctacaatga cacggccatc agcatcagag ataacactgt ccatttcttc accaacttca
     4381 gattcgctgg caatctgatc gttgcttgtc gcattgactg aatctgataa aagagctaga
     4441 accccttctt tcccaatctc agctaaacgt agcaaatccg tctgataacc ttctcttgct
     4501 gcttgatcaa atttgaagtc accggtataa acaatgtttc ctttatctgt tccaataaca
     4561 atacccatac tttctgggat tgaatgcgtt gttctaaaga acgaaactag gccatcttta
     4621 aattcaattt cagtatcact gtctaccaca tgaaaattat taaatttctt agtgctgtta
     4681 ttacttttca caaatagctt ggctaattca atagtcaatt cagatccaaa tacaggagct
     4741 gatacttcag ctaacaaata aggaagggct ccgatggcgt cagcatggcc atgactcaag
     4801 aaaatccctt gtactttacc tttgttttca attacataat ctaaattggg gatgactaaa
     4861 tctaccccaa gttgctcatt ttcggggtac ttgagccctg cgtctaaaat aaacatagaa
     4921 tcgttaattt ctaccaaata gaagtttttt ccgtattcac ggacccctcc caaggcaatc
     4981 attttaatat cagtcatgat actcctttaa atattttatt attacaaacg gtccttacaa
     5041 cgtaagtcga attactgtta tgtagctatg ctactatctt ctatagtata tcatatttga
     5101 ggcgtttttt ctctaaatag ccttgatact ttcagattat tttcagtacg gaaatcaaaa
     5161 aatcactgac ttattattcg tcaatgattt agatcgttga ttaatcaaat agttggtaat
     5221 agtcagtaat cgtcagttgt tctttttcca tttgatttaa atctttaata atgtttccat
     5281 ctttcattac aatcagacga ttcccataag ttaatgcatc ttccatatgg tgggtaatca
     5341 tcaaagctgt taagccatct tttgtcacaa actcatctgt caattgcatt aaagattgac
     5401 tcgtcttagg gtcaagggca gccgtatgtt catctaataa taagagagct ggctttttca
     5461 acgttgccat taataaactt aaagcctgtc tttgtccacc agacaaaaga cctgctggtg
     5521 tttctaaatg cttttctagg ccatttcctg ttcgtttaac caaatcctca aaactagcta
     5581 agtgctctgt tatttttcga gaagccaagg agcgtttttc tcctctttga cgtgcgatta
     5641 agagattttc agccactgtc atccgagggg ctgttcccat tttgggatct tgaaagactc
     5701 gcgataggta aagagctctt ttttctgccg gccaatgcgt cacatcctga ccaagaatcc
     5761 taatttgccc ccgtgtcaaa cttagcgttc ctgcaattac attaaaaagg gttgattttc
     5821 cagctccatt tcctcctaaa atagttaaaa aatcatgttc atagatagtt agtgtcacat
     5881 tgtccaatat tgttttagca tcttcaaaac cattatctac gtcaactgtt gcattgatca
     5941 attcaataat ttttgtcatc gtgttaacct cactccctta aagtagcgct cttttaccac
     6001 tggtatcatg aggcacagtg ctaaaacaag agcactaatt aatttcaaat aacttgtatt
     6061 aaaacctagc gtaataacta ctgaaattaa aaattgataa agaatcgagc ctattacaat
     6121 agcaatcaaa cgttctaaaa gagtcagtcc agttgagtat agtacttcac caacaattat
     6181 gctcgccaaa ccaataacaa taactccaat cccttttgag acatctgcat agccatcttg
     6241 ttggctcact agagcacctg ataaagctat taaactatta gaaaccacaa gtcccataac
     6301 ttccatccaa tccgtgttaa taccaaaact cttagccata tctttattgt ctcccgtagc
     6361 gatataagct tgacctagat tagtgtaaag gaaatagatc aaaacgctaa taacaatcac
     6421 aacagttatc agacctgtta aaaggctatt taaatcaatt gaaaaaggta aacaatcctg
     6481 aatacgttta tgatcatgta aacccaagtt tgctcttccc attaccatta gcataatgga
     6541 gttacatgac gtcatcacca aaattccagc taaaagtgtt ggcatttttc ctttcgtata
     6601 taacagccct gttaaaaaac ctgctaaagc acctgataac ataccaagta aggttgatag
     6661 aaatggattc caccctaagc taatagcggt gaccgcaaca gctcctccta aaggaaaaga
     6721 accttctgta gtcatgtcag ggaagtttaa aatcctaaag gtcaggtaga ttcctaatcc
     6781 taaaactccc catataaggc cttgtgaaac tgatgaaata atcatatttt tcctcatatt
     6841 tatattagcg ggtaactctc gccagtataa accctttctt tacattaatt tttagatttg
     6901 ttttatactt agttcagcac cgtggcttag tcttttttcc aatcacttta acgaaactaa
     6961 gacagaaaac aaaaaaatcg agaatcatgt ttaaacttac tcaaaaggca agatttttta
     7021 cgtgtctgaa atagagcatt ttaaaactaa aaatgataaa aataaccctt acatcctcaa
     7081 gacgattgtc tgacttggca tccaaattca tctcagtgag ggtgtttttt atggtttttt
     7141 cttgaccttt tatatcacat gtcattgtat taattctcaa taaaagaagc tgaaggaaaa
     7201 tcttccctgt taatagcaat acctaattct gctgctacat tcttattaat tagcggttga
     7261 ccaatatcca caactttgac aggaacatct acaacacgct ttcctttgat taattttaac
     7321 acctgattag ctgtttgaac tcccaaatcg tactgatttt gagaaatagc tgctaaacca
     7381 cctttttcaa ccattcgatc aacgctagtg tagacaggaa tctttgcagc cttactggtt
     7441 gtcatgactg atgaaaatgc acttgctata gtattgtctt gggggataaa taccgcgtct
     7501 actttaccta acataactga catcgtggca ggaacttcgt tagtagaagg aaccgcgtat
     7561 gaaatgactt gatagccctt tttgcgagct aaccgcctaa actctttaac ttgagataag
     7621 gaattatcct cattacttgc atataaaatt ccaactgttt tgacatgtgg tgtcagcttt
     7681 ttcattaaat caattgtctg tttaacaggc actttattag atagaccagt tacattagct
     7741 tcaggttgat ccaattgcat cactaatcta gatcctactg gatcagatac tgctgacatg
     7801 acaactggga tatccttgct ggtggctgct aagccttgcg ccgccggggt tgcgatacca
     7861 atcacgatat cacttcctga ttgcactaat tgcctactca ttgtttggat tttactctga
     7921 tccccctcag cattcataac cttaatcact acattttgtt tttgaggcat ctgctttttg
     7981 agttgatctt caatacctcg cttaatatca tcaagagcct catgagtaac gaattgtaag
     8041 ataccaattg taatgttttg tcggttagcc aaatcacggt tttcctttga tactcctttt
     8101 gataatagag agcctatcac tattacagtt aaaacaagga gggtagctat taaactcttg
     8161 tttttcatct gaattcctca catttcatgt caatctcttt aaaacgttga tgatgagaat
     8221 acttctcaaa agaaaaagca cttagtaatg attactaggc gcccttgtga aatccaatgt
     8281 cagcgcatag atatcttttt agaagtactc taaaagtcgt atctatgcca attcataaac
     8341 acaacttatc tatgccagcc ttgccaattt actaagtgtt gtgtttttac catcattgac
     8401 ttcctttctc gtttttttaa cattataacc taaatcaaaa aaattgcaag ctctttcgtc
     8461 catattttta gtcaagtatt tgtggtataa tgaagagatt agaattcaga aaggagaaac
     8521 catgtctttt gacggctttt tcttacacca tttaaccaac gaattaaaag agaacttgct
     8581 ttacggtcgt atccaaaagg tcaatcaacc ctttgaacgg gaacttgtct taaccattcg
     8641 taaccatcgc aagaattata aattgctatt gtctgcccat cctgtttttg gaagggtaca
     8701 aatcacccaa gcagactttc aaaatcctca ggtacctaat acctttacca tgattatgcg
     8761 taaatacctc cagggtgcag tcattgagca gttagaacag attgataatg accgcattat
     8821 cgaaatcaag gtatctaata aaaatgaaat cggagatgct attcaggcaa ccctaatcat
     8881 tgaaatcatg gggaagcaca gcaatatcat cttggtggac cgtgcagaaa ataaaatcat
     8941 cgaatccatt aaacatgttg gcttttcaca aaactcttac cgcaccattt tacctggctc
     9001 tacctacatt gagcccccta aaacagctgc tgttaatcct tttacaatta cagatgttcc
     9061 tctatttgaa atcctccaaa cacaagaact aacagttaaa agcttgcaac agcattttca
     9121 aggtttgggg cgagacaccg caaaagagtt ggccgagctt ctgaccacag acaaattaaa
     9181 acggttccgt gagttttttg ctagacctac tcaggcaaat ctgaccacag cttcttttga
     9241 gcctgttctt ttctcagata gccatgcgac gtttgagact ttatcagaga tgcttgatca
     9301 cttttatcaa gataaggctg agcgtgaccg tatcaatcag caagccagtg atttgattca
     9361 ccgtgtgcaa actgaactgg acaaaaatcg taacaagcta agtaagcaag aagctgagct
     9421 gctagccact gaaaatgctg aattattccg tcaaaaagga gaattattga ccacctacct
     9481 cagtctggtc ccaaacaacc aagactctgt gattttagac aattattata caggggaaaa
     9541 aattgagatt gccttagaca aggctctgac accaaatcaa aatgctcaac gttattttaa
     9601 aaaataccaa aagctaaaag aagctgtcaa acacttatct ggtttgattg ccgataccaa
     9661 gcaaagtatt acttactttg agagcgtgga ctacaattta tctcaagcaa gtatcgacga
     9721 catcgaagat attcgcgaag aattatatca ggctggcttt ttgaaaagtc gccaaagaga
     9781 caaacgccat aaacgtaaaa agcctgagca gtacctggct tcagatggaa aaaccattct
     9841 catggtggga cgcaataact tgcaaaatga agaactcacc tttaaaatgg ctaaaaaagg
     9901 agaactctgg ttccatgcta aggatatccc tggaagtcat gtcatcatca aagacaatct
     9961 tgacccaagt gacgaggtta aaactgatgc tgccgaacta gctgcttatt attcgaaagc
    10021 taggttatca aatctcgttc aggtcgatat gattgaagct aaaaaattac acaaacctag
    10081 tggcgccaag ccaggatttg tgacttatac tggtcaaaaa actttacggg ttactcctga
    10141 ccaagccaaa attctctcta tgaaattatc ctgataagca actaaaaaac agctctccta
    10201 ggaaagttat ttcccctagg agaactgttt ttttattctg attgttggtg cttgatatgg
    10261 tcaagaaagt cttgcatatt cacatcatca ggcacttgtc tgaggtaagc ggtagccatt
    10321 tggatagcct tgtgaaactg gccaaactcc cttaataaat aggcataatc ctgtaaaaac
    10381 tccggatttt ctgccaaatc agccgaaatc tcattataaa gagccaaggc aacttcttct
    10441 tgttccaagg cgtggtaagc ctttgcaatg gtccatttgg ttaagacatt atcaatcgtc
    10501 tcacgattaa gagcaataac ctcctcaaaa cgctcggcat caaaataaag ggtcactaaa
    10561 cgcatgagaa tctcttcgtc atcaacagct acttcctttg cttgaagcag gtaattttca
    10621 gcattctgcc tatcgtgtaa ttcataagaa agctgcgatg ctagtaataa taattgactg
    10681 tcaaatgcat ttttacgtag gccctgttga accaagcgca aagcttctga cgtcttatgc
    10741 tcttcatgaa gactcaaagc ataaccatat tcataacctg gataatctgg attgatggtt
    10801 tccaactgtt taaagtaaag attagctttt tgataatttt cttggtcata caagagggtc
    10861 gccaattcaa aaacagtttc atcttcgtat tcaatggcca cggccttttc caagaattcg
    10921 atggctgcct caaatttccc taagctagca taggctcgtc caatgcgttg ataagtggaa
    10981 atacctgtca attcaaggat ttgacgattg tctaactgtg catagtaatc aatagcttct
    11041 ttaaaatact gcaaggacat gtcaatttct gccaaaccaa aaatgaccaa tggctctggg
    11101 ctgatgctca ctgcttgaag caatttttca cgcgccacct ctgtcagtcc ttccatgtca
    11161 tagaggtcag ccattactaa gagagctgac aaataattgg ggctatcttt tgaaacctta
    11221 tccaaataca agaaagcttc ttcaatggcg tcgtcttctg ctgcaatctg agctaaattt
    11281 atattgagtt ctgggtaatc atcagctaac tgcaaataaa tacgcttggc atgtggcaaa
    11341 aaacctatac tttctaagta ttctcctaag gcgattaaac tatctgcatc atcttctttt
    11401 aaagcttttt ggaaatattt ttcagcgtgc gctaggtctt gctgatccaa ggaagcaatc
    11461 attttttcac tatttaacat ggtccttaac ctcctcaatc accaatactt cttcgtcata
    11521 caaaccgcta acttttttgt accagtcaaa taattcttta atcacaactt tgacagaagc
    11581 gtagattggg atgactaaga agacacccca aacgccaaac attgatcctg cagtcaacaa
    11641 taagaacatg atcgtaatcg gatgaatgct cagcttattt ccaagaacaa gcggtgccac
    11701 aaaacggcct tcgatggttt gctcaatcat aaagataaca agcaccttaa ccaacataaa
    11761 tgggccttgt accatagcca taatcacaac aggtatcatg gcaaggaaac ttcctaagta
    11821 aggtatcatg ttaagaaagc cagcaataat cccaaaagtg atggcatatt tcagtcccac
    11881 aaggctaaac atgatagaaa acataaagcc gacaacaatt gcaaccgtaa cctgcccttg
    11941 gacatagcct gataattgct tgttaatatc tcccaagact cgaaccatag gcacccgtaa
    12001 tttgagaggt aagacattca caaggccatt tttcatgtgg ctactgtctc gtaaaaaata
    12061 aaagagaata aagggcgaaa taataatagc cacggtaatc cgagcaatgg caccagcaaa
    12121 attaccagcc cagtcaatag cattttttga aaaactttgc gcgtaatcca aagccttttg
    12181 acttgtatgg gtaagcatgt cttggagttg gggcttgtag ctgactaacc attcattttc
    12241 aagaagttta ttggcttctt cattgacctt gcccacatat ttgggcaaat cctcaataaa
    12301 actggtaagt tgggtctgta ccatgggaac aaagcctgaa atcgcccaga caaaaagtaa
    12361 ggtaatcaaa ccaaaaacaa taaaaataga ggtggtacga ttcgggccca aatgattaat
    12421 aaggtcaact aagggtttgg tcagataata cagaatggtc gaaataacca agggcaacat
    12481 gatgaccgca aaaaaagaaa tcactggcat aaataaaaaa gagattttag taaaaacaaa
    12541 aatcgttaaa aaggctaaaa gtgttattac taaagccatg gtagcttgat tatttaaaaa
    12601 ccatttgtaa aataagctat ctgttctaga cttttcaggt attaagggca ttaagatagg
    12661 gttcctttct ttgggaaaat gataagtttt actggtaacc tgggtaaatc aataaaaggt
    12721 cacagattta tccatgagtt ggttgttttg gtcataggta aatttggcag agaaaaaagg
    12781 acggtcttct aaaatccatt taaaaatgtc ataatcacct tcccaagttg gcttttccaa
    12841 cacttgatcg taaggtaccc attccaaggt tccttctcgt gactcttcat ctgaaatgag
    12901 gtctccttca aatcctgtta ctttaaaaac ataggtatac cagtcatgac caggggtaaa
    12961 ttctggaaaa gtaatgattc ctttaaaggc catctctgta acagtcaaat gggtttcctc
    13021 taaaatctca cgacgggcac actcatctgg ggtctcacca gcctcgagct tacccccaac
    13081 tgaaatccac ttgcctttgt ggacatcatt ttctttttta ttgcggtgta ataatagcaa
    13141 agaatcgcca ttatcaatgt aacaaatggt tgctaattga gtcatctatc tctttccttt
    13201 ccatctttgt ttctattata ccaatctcta gaaaatatgc tatcatttta tgagtcaaag
    13261 tgtgaatact tagtcacgct caaaaccttc taaagatgcc ttaaaaaagt gtagataaga
    13321 gcctacaatg aaaaacatca atttaaacta tcaa
//
LOCUS       DAUDYX010000052        12934 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.053, whole genome shotgun sequence.
ACCESSION   DAUDYX010000052 DAUDYX010000000
VERSION     DAUDYX010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12934
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.053"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(382..2958)
                     /locus_tag="VHD02_001561"
     CDS             complement(382..2958)
                     /locus_tag="VHD02_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEQ5498156.1"
                     /translation="MKNNNKWIIAGLASFLFPLSIIFIILLSMGIYYNSDKTILASDA
                     FHQYVIFAQNFRNIMHGSDSFFYTFTSGLGINFYALMCYYLGSFFSPLLFFFNLTSMP
                     DAIYLFTLIKFGLIGLAACYSFHRLYPKINAFLMISISVFYSLMSFLTSQMELNSWLD
                     VFILLPLVVLGLNKLITENKTRTYYLSISLLFIQNYYFGYMIALFCILYALVCLLSLN
                     DFNKMFIAFVRFTAVSICAALTSALVILPTYLDLSTYGENLSPVKQLVTNNAWFLDIP
                     AKLSIGVYDTTKFNALPMIYVGLFPLMLSVIYFTLESIPLKIKLANACLLTFIIISFY
                     LQPLDLFWQGMHSPNMFLHRYAWSFSIVILLLACETLSRLKEVTQIKAGFAFIFLIIL
                     TSLPYSFSQQYNFLPLTLFLLSVFLLLGYTISLFSFRNSQIPPTFISAFILIFSLLES
                     GLNTYYQLQGINKEWGFPSRQIYNSQLKDINNLVNSVSKNSQPFFRMERLLPQTGNDS
                     MKFNYYGISQFSSVRNRLSSSLLDRLGFQSKGTNLNLRYQNNTIIMDSLLGIKYNLSE
                     GPLNKFGFTKLKTSGNTTLYQNHYSSPLAILTRNVYKDVNLNVNTLDNQTKLLNQLSG
                     KSLTYFNLQPAQLISGANQFNGQISAQASDYQNSVTLNYQINIPKHSQLYVSIPNIIF
                     SNPDAKEVRIQTDNHNFIYTTDNAYSFFDLGYFADAKVATFSFVFPKNKQISFKEPHF
                     YSLSIESYLEAMNSIKQKNVHTYAKSNTVITDYNSKTKGSLIFTLPYDKGWSAQKDGK
                     NLPVKKAQGGFLSVTIPKGKGRVILTFIPNGFKLGLSLSCVGIIAYMLLYKYIDIKSK
                     LL"
     gene            complement(3041..4660)
                     /locus_tag="VHD02_001562"
     CDS             complement(3041..4660)
                     /locus_tag="VHD02_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ5498157.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPSTGHISLGPDERLSVLRQNHFDYEEERAIDVVIMGNEQLYNIMKEKDAI
                     YMKADFSEEDGVRAAELEGIFAELGGWEAESEASQLLQNLNIPEDLHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSISWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKQSSELAARLQADRNAKAEEKIKELQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREMGNDFLTVENLSVTIDGEKI
                     IDNISFILRPGDKAAIIGQNDIQTTALMRALADDIDYEGTIKWGVTTSRSYLPKDNSK
                     DFATEESILEWLRQFASKGEDDDTFLRGFLGRMLFSGDEVKKSVNVLSGGEKVRVMLS
                     KLMLLKSNVLILDDPTNHLDLESISSLNDGIKDFKESVIFASHDHEFIQTIANHIVVI
                     SKNGVIDRIDETYDEFLDNPEVQARVAELWK"
     gene            complement(4739..5611)
                     /locus_tag="VHD02_001563"
     CDS             complement(4739..5611)
                     /locus_tag="VHD02_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ5498158.1"
                     /translation="MKKRGIDLLLVTIGSFITAIGFNTMFVDNHIASGGMVGIAVVIK
                     ALFGISPSLFLMASNIPLLLMCYFFLGKQNFIKTLYGSWIYPIAIRSTNSLPTLTHNQ
                     LLAAIFGGIICGIGLGMVFWGNSSTGGTGILTQILHKYSPLSLGVAMTIVDGISVLMG
                     FIALSADDVMYSTIGLFVIGYVISVMENGFDSSKNVMIISKDYQAIREYITTVMDRGV
                     TKLPIRGGYTTSDKIMLMAIVSSHELPTLQEKILEIDDTAFIVVMPAAQVMGRGFSLT
                     KQYKREDKDVLLPM"
     misc_binding    5734..5975
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6030..7052
                     /gene="trpS"
                     /locus_tag="VHD02_001564"
     CDS             6030..7052
                     /gene="trpS"
                     /locus_tag="VHD02_001564"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEQ5498159.1"
                     /translation="MTKPIILTGDRPTGKLHLGHYVGSLKNRVFLQNENKYKMFVFLA
                     DQQALTDHAKESELIQESIGNVALDYLSVGLDPKQSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPSGFLVYPVSQAADITAFKANLVPVGNDQKPMIE
                     QTREIVRSFNHTYHTDCLVEPEGIYPENEKAGRLPGLDGNAKMSKSLGNGIYLSDDAD
                     TVRKKVMSMYTDPNHIKIEDPGQIEGNMVFHYLDIFARKEDQADIEAMKEHYQRGGLG
                     DVKTKRYLLDILERELAPIRERRLEYAKDMGEVFRMLQEGSQKARTVAAKTLSEVKSA
                     MGINYF"
     gene            7360..8841
                     /gene="guaB"
                     /locus_tag="VHD02_001565"
     CDS             7360..8841
                     /gene="guaB"
                     /locus_tag="VHD02_001565"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEQ5498160.1"
                     /translation="MSNWDTKFLKKGYTFDDVLLIPAESHVLPNEVDLKTKLADNLTL
                     NIPIITAAMDTVTGSKMAIAIARAGGLGVIHKNMSITEQAEEVRKVKRSENGVIIDPF
                     FLTPEHKVSEAEELMQRYRISGVPIVETLANRKLVGIITNRDMRFISDYNAPISEHMT
                     SEHLVTAAVGTDLETAERILHEHRIEKLPLVDNSGRLSGLITIKDIEKVIEFPHAAKD
                     EFGRLLVAAAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIYQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAASDIVFQMLGGIRSGMGY
                     VGAGDIQELHENAQFVEMSGAGLIESHPHDVQITNEAPNYSVH"
     gene            9044..9907
                     /locus_tag="VHD02_001566"
     CDS             9044..9907
                     /locus_tag="VHD02_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEQ5498161.1"
                     /translation="MEGIFYALIPMFTWGSIGFVSNKIGGKPSQQTLGMTFGALLFSL
                     AVWLIVRPEMTLQLWLFGILGGFIWSIGQTGQFHAMQYMGVSVANPLSSGSQLVLGSL
                     IGVLVFHEWTRPMQFVVGSLALLLLIIGFYFSSKQDDANAQVNHLHNFSKGFRALTYS
                     TIGYVMYAVLFNNIMKFEVLSVILPMAVGMVLGAITFMSFKISIDQYVIKNSVVGLLW
                     GIGNIFMLLAASKAGLAIAFSFSQLGAIISIVGGILFLGETKTKKEMRWVVTGIICFI
                     VGAILLGVVKS"
     gene            complement(9964..11070)
                     /gene="recF"
                     /locus_tag="VHD02_001567"
     CDS             complement(9964..11070)
                     /gene="recF"
                     /locus_tag="VHD02_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEQ5498162.1"
                     /translation="MWIKELELKHYRNYDHLLASFSSGLNVFIGNNAQGKTNFLEAIY
                     FLSLTRSHRTRADKELIHFDHSTVSLTGKIQRISGTVDLEINLSDKGRVTKINALKQA
                     KLSDYIGTMMVVLFAPEDLQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQR
                     NSYLKSAQQIDAAFLAVLDEQLAGYGARVMEHRIDFINALEKEANTHHQAISNGLESL
                     SLSYQSSVVFDKKTNIYQQFLHQLEKNHQKDFFRKNTSVGPHRDNLAFYINGMNANFA
                     SQGQHRSLILSLKMAEVSLMKALTGDNPILLLDDVMSELDNTRQTKLLETVIKENVQT
                     FITTTSLDHLSQLPEGIRIFHVTKGTVQVDSDIH"
     gene            complement(11072..11485)
                     /gene="yaaA"
                     /locus_tag="VHD02_001568"
     CDS             complement(11072..11485)
                     /gene="yaaA"
                     /locus_tag="VHD02_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEQ5498163.1"
                     /translation="MIYKLFTEFITLQALLKELGIIQSGGAIKGFLAETTVLFNGEDE
                     KRRGKKIRIGDKISLPDQDLIITIVEPSQEEKEQFAEEIAEKTRVAALVKQMNQANKK
                     TSSKHKNRQSTTKKSLRATKKTKGKPTAPVRFPGI"
     gene            complement(11793..12707)
                     /gene="hasC"
                     /locus_tag="VHD02_001569"
     CDS             complement(11793..12707)
                     /gene="hasC"
                     /locus_tag="VHD02_001569"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     HasC"
                     /protein_id="HEQ5498164.1"
                     /translation="MTKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALKSGIEEILVVTGKAKRSIEDHFDSNFELEYNLQAKGKNELLKLVDETTAINLHFIR
                     QSHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNASAKPLTKQLMEDYDKTHASTI
                     AVMKVPHEDVSSYGVIAPQGKAVKGLYSVDTFVEKPQPEDAPSDLAIIGRYLLTPEIF
                     DILERQTPGAGNEVQLTDAIDTLNKTQRVFAREFKGNRYDVGDKFGFMKTSIDYALEH
                     PEVKEDLKNYIIKLGKALEKSKVPTHSK"
BASE COUNT         4154 a         2118 c         2441 g         4221 t
ORIGIN      
        1 caggattgta ttttgcataa aaatagaagt tctgttcttt aattgtatag atgataacat
       61 taagtttaat tacaaacatt aactgaagct taatttttta cattttgaaa gcatgtctcc
      121 catttaatac tgatgaatat aagattattg taaggagtca aaagattgaa atcgtatcag
      181 cgatgattat tgtcatttca atctttgaaa gagagtagtt taacattaac cctcacgaaa
      241 aatctgacct ttatattatt tgagtttatt agtaaaatcg ataaaagcat ctcaagtatt
      301 agaatatttg tctagctctt gagatgcttt tagtatattg actcatttgc caaattgata
      361 aaaaaatctg atttttttgg actaaagtaa tttagacttt atatctatgt acttatacaa
      421 aagcatataa gcgataattc ctacgcaaga tagagataac cctaatttaa aaccattagg
      481 aataaaggta aggataacac gtccctttcc tttaggaata gtaactgata gaaatcctcc
      541 ttgtgctttt ttgactggaa gatttttccc atctttttgt gctgaccaac ctttatcgta
      601 aggaagtgta aaaataagag aacctttcgt ttttgaatta taatcagtga ttaccgtatt
      661 acttttagcg taagtatgaa catttttttg tttaatacta ttcattgctt caaggtaaga
      721 ttcaatagac aaactataaa aatgaggttc cttaaaacta atttgtttat tttttggaaa
      781 aacaaacgaa aatgtagcaa ctttggcatc ggcgaaatat cctaaatcaa aaaaagagta
      841 agcgttatct gtagtatata tgaaattatg attatctgtc tgaatacgca cctctttagc
      901 atcaggattt gaaaatataa tattgggtat gctaacatag agttgactat gtttagggat
      961 gttaatttga taattaaggg taacggagtt ttgataatca gaagcttgtg cagatatttg
     1021 tccgttaaat tgattagcac cagaaataag ttgagctggc tgtaagttaa aataggttaa
     1081 agatttccca cttagttggt taagtaattt ggtttggtta tcaagggtat tgacatttag
     1141 attgacatct ttgtaaacat tacgtgttaa tatagctaaa gggctactat agtgattttg
     1201 ataaagagta gtattcccgc tagtttttag ttttgtaaat ccaaatttat ttagaggtcc
     1261 ttcgctaaga ttatatttta taccaagtag actgtccata ataatagtat tgttttggta
     1321 tctaaggttt aaattcgtgc ctttagactg aaatcccaat cgatccaata aagaactaga
     1381 tagtctattt cttacagagg aaaattgtga aatgccgtaa taattaaatt tcatgctatc
     1441 gttccctgtt tggggaagta gtctttccat tctaaaaaaa ggttgactat tttttgacac
     1501 agagttgaca aggttgttaa tatcctttaa ttgactatta tatatctgtc gtgatgggaa
     1561 tccccactcc ttattaattc cttgaagctg gtagtaggtg tttaaccctg attcaagaag
     1621 gctaaagata agtatgaaag cagaaataaa agtaggtggg atttgagaat ttctaaacga
     1681 aaatagtgaa atagtataac ctaataataa aaaaacacta agtaaaaaaa gagttaaagg
     1741 tagaaaatta tattgttgag aaaagctata aggaagagat gtcagtataa tgaggaaaat
     1801 aaaagcaaaa cctgctttta tttgagtcac ttcttttagt cgagagagag tctcacatgc
     1861 gagtaatagg ataactatgg aaaaagacca agcgtagcga tgcaaaaaca tatttggtga
     1921 gtgcatcccc tgccaaaaaa gatcaagtgg ctgtaggtaa aaacttatta taataaaagt
     1981 taacaagcag gcattggcta attttatttt taaagggata ctttctaaag taaaataaat
     2041 aacactaagc attaggggaa ataatcctac gtaaatcata ggcagagcat taaacttggt
     2101 agtatcgtac actcctattg agagcttagc aggtatatcc aaaaaccaag cattgttcgt
     2161 aactaactgt tttaccgggg atagattctc tccataagtt gacaaatcta gataggtagg
     2221 aagtattacc agagcacttg ttaaagcagc acatattgac acagctgtaa acctaacaaa
     2281 agcgataaac attttgttaa aatcattgag acttaaaaga caaactaagg cgtaaagaat
     2341 acaaaaaaga gcaatcatgt agccaaagta gtaattttga atgaataata atgatatcga
     2401 aagataataa gttctggttt tattttctgt gataagttta tttaatccaa gtacaacaag
     2461 tggaagaaga atgaaaacat ctaaccaaga atttagttcc atttgacttg tcaagaagct
     2521 cattaagcta taaaaaactg agatggaaat catcaagaaa gcattgattt ttggatataa
     2581 tctatgaaaa gaatagcatg cagctaatcc tattaaccca aattttatca aggtaaacaa
     2641 atagatagca tctggcatag aggttaaatt aaaaaagaaa agtaatggag aaaagaaact
     2701 gccaagataa taacacatta aagcataaaa atttatccct agtccgcttg taaaggtata
     2761 aaaaaaacta tcagaaccgt gcatgatgtt acgaaagttc tgcgcaaaaa taacatactg
     2821 atgaaaagca tcactagcta gaattgtttt atcactatta taataaatgc ccatcgatag
     2881 aaggatgata aatataatac taagagggaa caaaaaacta gcaagtccag caattatcca
     2941 tttattatta tttttcataa attatatggt ctttcgttta caaattttaa aaaagaggct
     3001 aggaagagat cttcttagcc acctcattta tcataaggtt ttatttccaa agttcagcaa
     3061 ctcgggcttg gacttctgga ttgtcaagga attcgtcata agtttcatct atacggtcga
     3121 taacaccatt ttttgaaata acaacgatgt ggttagcaat cgtttgaata aattcatggt
     3181 catgactggc aaagataaca gattctttga agtctttgat cccatcatta agacttgaaa
     3241 tagattccaa atccaagtga tttgttggat catcaagaat aagaacgttt gatttcaata
     3301 gcatcaactt agaaagcatg acacgcactt tttcgccacc ggaaagaacg ttaacagatt
     3361 ttttaacttc gtctccagag aaaagcatcc gtcctaggaa accacgtaaa aaagtatcgt
     3421 catcttcacc ttttgaggca aattgacgta accattcaag aatagattct tccgtggcaa
     3481 aatcttttga attgtcttta ggcaagtaag aacgacttgt tgtcactccc catttaatgg
     3541 ttccttcgta gtcaatatca tctgctaaag cacgcataag agcggttgtt tgaatatcat
     3601 tttgaccaat aatagcagct ttgtcacctg gacgtaaaat gaaactgata ttatcaatga
     3661 ttttctcccc atcgatcgtt acagaaagat tttcaacagt gaggaagtca ttccccattt
     3721 cacgctctgc tttaaaatta ataaatgggt atttacggct tgagggaaca atttcttcta
     3781 attcaatctt atcaagcatt tttttacgag aggttgcttg ttttgattta gatgcgtttg
     3841 ctgagaaacg ggcaacgaat tcttgtaatt ctttgatttt ttcttcggct ttagcattgc
     3901 ggtcagcttg taaacgagcg gctagctcag aagattgttt ccagaaatcg tagttaccca
     3961 caaagagttt gattttacca aagtcaaggt cggccatgtg agtacatact ttattcaaga
     4021 agtgacggtc atgggaaaca acgatgactg tattttcaaa atcaatcaag aaatcttcta
     4081 accatgaaat agattgaata tcaagaccat tggttggctc gtcaagtaat aaaacgtcag
     4141 gcttaccaaa gagcgcttta gcaagaagga ctttcacttt atctccatta gccagttcac
     4201 tcatattttg gtagtgaagg tcttctggaa tatttagatt ttggagaagt tgagaagctt
     4261 cactttctgc ctcccagcca cctaattcag caaagatacc ttctagctca gcggcacgaa
     4321 ccccatcttc ttctgaaaaa tctgctttca tatagatagc gtctttttct ttcatgatat
     4381 tgtaaagttg ctcattaccc atgattacaa catcaatcgc acgttcttct tcataatcaa
     4441 aatgattttg gcgaagaaca gataagcgtt cgtcaggtcc taaagagata tgaccagtac
     4501 ttggttcaat atctcctgca aggattttaa gaaaagttga ttttccggca ccattcgcac
     4561 cgattaagcc gtaagtattg ccggctgtga atttgatatt aacatcatca aatagttttc
     4621 gatcactaaa gcgtagtgat acatcagaaa ctgtaagcaa tttgtcacct cattttattt
     4681 atctaagctt cattgtacct aaaatagtcc cctttttcaa tcagaaaagg gctaatcact
     4741 acataggcaa taaaacgtct ttgtcttcac gtttgtattg tttagtcaaa ctaaatcctc
     4801 ttcccataac ttgagctgct ggcatgacaa caataaaggc tgtgtcgtca atttcgagga
     4861 ttttttcttg gagagtgggt aattcgtgac ttgatacaat agccattaac ataattttat
     4921 cagaagtagt ataaccaccg cgaataggaa gtttagtcac tccacgatcc ataacagttg
     4981 tgatatattc tcgaatagct tgataatctt tggaaataat cataacattt ttggatgagt
     5041 caaagccatt ttccataacg ctaatcacat aaccaattac gaaaagtcca atcgtagaat
     5101 acatcacgtc atctgcagaa agtgcaataa atcccataag gacactaata ccatctacaa
     5161 ttgtcattgc aactccaaga gataaaggag agtatttatg aagtatttgt gttagaattc
     5221 cagttcctcc tgtcgaagag tttccccaaa aaaccattcc taaaccaatg ccacagataa
     5281 taccaccaaa aatagctgct aacaactgat tgtgtgtcag agtaggtaat gaattggttg
     5341 aacggatggc aataggatat atccaagaac cataaagtgt tttaataaaa ttttgttttc
     5401 caagaaagaa gtaacacatt aataataagg gaatattact agccattaaa aaaagtgacg
     5461 gactaatgcc aaatagagct tttataacaa ctgcaatacc aaccataccc cctgatgcaa
     5521 tatgattgtc gacaaacatt gtattgaaac caatagctgt tatgaatgag ccaattgtga
     5581 caagtagtaa atcaatccct cgttttttca tgcttttaat ctcctttata actggttaaa
     5641 aatagtagac tcattatctc ttaaatgtac tgagaccgtc aagtcatatt ttcaaaaaat
     5701 ctggtgaact tgacaagaag tttaaaattt catagaataa atttgaatta gaagagtatt
     5761 cttttcatgt ttttagagag ttagtggttg gtgcaagcta acaacagaaa aggtgaaatg
     5821 gactaatgat tatttgaaag gaaaccataa ctttcaggtt ggcacccctt accgtgcagc
     5881 ttctttcatt gagaagattg agatagtatc tcgttttgat aaagcagatg ctaattgagg
     5941 tggtaccgcg tattacttgt aataacgccc tcacgtttta atagcgtggg gactttttgc
     6001 tatatcaaga gatagttagg gagaaaatga tgacaaaacc tattatttta actggagata
     6061 gaccaactgg aaaattacat ttaggtcatt atgtcggaag tcttaaaaat cgtgtctttt
     6121 tacaaaatga aaacaagtat aagatgtttg ttttcttggc cgatcaacag gcactgacgg
     6181 atcatgctaa agaatccgaa ttaattcaag aatctattgg aaatgttgcc ttggattacc
     6241 tctctgtcgg tttggatccg aagcaatcaa cgatttttat tcaaagtcag attccagagc
     6301 tagctgaatt gagcatgtat tatatgaatc tggtatcatt agcacgtttg gaaagaaatc
     6361 ccactgttaa aaccgaaatt gctcagaaag gttttggcga aagtattcca tctggttttt
     6421 tggtttatcc agtatcacag gccgctgata ttacagcatt taaagcgaat ttagtacctg
     6481 taggtaacga tcagaaaccg atgattgaac aaacacgtga aattgtgaga agttttaatc
     6541 atacttacca tacagactgt ttagtagaac ctgaaggtat ttatccagaa aatgaaaaag
     6601 ctggacgctt acctggtctt gatggcaatg ccaaaatgtc taaatcattg ggaaatggaa
     6661 tctatctttc agatgatgca gataccgttc gcaaaaaagt gatgagcatg tatactgatc
     6721 caaatcatat taaaatagaa gatcctggtc aaattgaagg gaatatggtc tttcattatt
     6781 tggatatttt tgctagaaaa gaagatcaag ctgatatcga agcaatgaaa gagcattatc
     6841 aaagaggtgg tttaggagat gtgaaaacga aacgctacct tctagatatt ttagaacgtg
     6901 aattagcacc tattcgtgaa agacgtttgg agtacgctaa agatatggga gaggtgttcc
     6961 gtatgttaca agaaggtagt caaaaagcaa gaactgtggc agccaagact ttatcagaag
     7021 tgaagtcagc aatgggtatt aattattttt aatgttttag agaaaaagct aagttagtaa
     7081 tgataagata attactatta tcaaagcagg acctaatttg tcatttcttt acaggatagt
     7141 agagattatg ttttatatag tcttattttt gattaaaaat aagacccaaa tgaatttttt
     7201 gattaataaa aaacggataa tactaatata ttatgctgat agtgacttca gctgtaggaa
     7261 tattttattg acaaatgatt ttagcgtgat atgatagtaa caataaaaaa tagaccaggg
     7321 taatattccc taaaaataaa agaaaagagg attgtctgga tgtcaaattg ggacactaaa
     7381 tttttgaaaa aaggctacac ttttgacgat gtcttgctta ttccggcaga gagtcatgtt
     7441 cttccaaatg aagttgattt aaagacaaaa ctcgcagaca atttgacatt aaatattcct
     7501 attattacag cagctatgga tacggttact ggtagtaaaa tggctattgc aattgcgcgt
     7561 gcgggtggtt taggtgttat tcataaaaac atgtctatta ctgagcaagc agaagaagtt
     7621 cgcaaggtaa aacgttctga aaatggtgtc atcattgatc cattcttctt aacacctgaa
     7681 cacaaggttt cagaagctga agaattaatg cagcgctacc gtatcagtgg tgtgccaatt
     7741 gttgaaactt tagcaaaccg taagttggtc ggtatcatta ctaatcgtga tatgcgtttt
     7801 atttctgatt acaatgcacc aatctctgaa catatgacta gtgagcacct cgttacggct
     7861 gctgttggta cagatcttga aactgcagaa cgtattcttc atgaacaccg tattgaaaaa
     7921 cttcctttgg ttgataatag tggtcgtctg tctggtttaa tcactatcaa agatattgaa
     7981 aaagtaatcg aattcccaca tgctgctaaa gatgaatttg gtcgtcttct ggtcgctgct
     8041 gcggtaggtg ttacctcaga tacttttgag cgtgccgagg ccttgtttga agctggtgca
     8101 gatgcgattg ttattgacac agctcatggt cattcggctg gtgttcttcg taaaattgct
     8161 gagattcgtg ctcatttccc aaatcgtact ttgattgctg gaaatattgc cactgctgaa
     8221 ggtgcgcgtg ctctttatga tgcaggtgtt gatgttgtta aagtgggtat tggtcccgga
     8281 tctatctgta ccactcgtgt ggttgcaggt gtcggtgttc ctcaagtgac tgctatttac
     8341 gatgctgctg ctgttgctcg tgaatatggc aaaacgatta ttgctgatgg tggtatcaag
     8401 tattctggcg atattgttaa ggctcttgca gccggcggta atgctgttat gcttggatca
     8461 atgtttgcag gtactgatga agctccaggt gaaactgaaa tctaccaagg ccgtaagttc
     8521 aaaacttatc gtggaatggg ttccattgca gctatgaaga aaggatcaag cgatcgttac
     8581 ttccaaggtt ccgtaaatga agctaataaa ctagttccag aaggtattga aggccgtgtt
     8641 gcttataaag gagctgcttc agatattgtc ttccaaatgt taggtggtat ccgctcaggt
     8701 atgggctatg ttggcgcagg tgacattcaa gaattacatg aaaatgctca atttgttgaa
     8761 atgtcaggtg caggcttgat tgaaagtcat ccgcatgatg tccaaatcac aaatgaagca
     8821 ccaaactatt ctgtgcactg ataatttaat ttagtaactt taagggctga catattgtgc
     8881 cagctctttt tttatattaa aaaatgataa aatataataa caattttcgg ataactttta
     8941 ttgttttttc ttagtaaata agctataatt agtctttaca tgaaaccatt ttatgataga
     9001 attattataa tctttaaaga tttactataa aggagataac attttggaag gtatttttta
     9061 tgcacttatt cccatgttta catggggaag cattggattt gttagtaaca aaattggagg
     9121 aaaaccttcg caacaaactc taggaatgac ttttggggct ttattatttt cattagctgt
     9181 ttggttaatt gttagacctg agatgacact gcaactttgg ctttttggaa tattgggtgg
     9241 ttttatctgg tcaattggtc aaactggtca atttcatgcc atgcaataca tgggagtctc
     9301 cgttgccaat cctttgtcaa gtggctctca acttgttctg ggaagtttga ttggtgtcct
     9361 cgtttttcat gaatggacaa gacctatgca atttgtagta ggaagtctag cactattact
     9421 tctgattatt ggtttttact tttctagtaa acaagatgat gctaatgcac aagtcaatca
     9481 ccttcataat ttttcaaaag gatttagggc gctcacttat tcaacaattg gttacgtgat
     9541 gtatgctgta ttatttaata acatcatgaa atttgaagtt ttgtcagtca ttttgcccat
     9601 ggcagtaggt atggttttag gagctataac tttcatgtca tttaaaattt ccattgacca
     9661 gtatgtgatt aaaaatagcg ttgttggatt actttggggc attgggaata tttttatgct
     9721 tttagctgca tcaaaagcag gacttgctat tgcctttagt ttctcacaat taggtgctat
     9781 catttcgatt gttggaggca ttttattcct aggcgaaacc aaaaccaaaa aagaaatgcg
     9841 ttgggttgtc acaggaatta tttgttttat cgtaggtgct attttattag gtgtggtcaa
     9901 atcttaatcg ccacataata tcaaaaaaag gctcagttta actgaaccct ttttttgatg
     9961 ttattagtga atatcagaat caacttgaac ggttcctttt gttacgtgaa aaatacggat
    10021 accttctggc aactgtgaca ggtgatccaa acttgttgtt gtgataaagg tttggacatt
    10081 ttctttaatt actgtttcaa gtaactttgt ttgtcgtgta ttgtctagct cgctcatgac
    10141 atcgtctagt aaaagaatgg ggttatctcc agtcaaggcc ttcattaaac taacttcagc
    10201 catttttaag gaaagaataa ggctgcggtg ctgaccttga ctagcaaagt tagcattcat
    10261 cccgttaatg taaaaggcta agttatcgcg atgtggacca acgctagtgt ttttacggaa
    10321 gaaatctttc tgatgatttt tttcaagttg gtgtaaaaat tgttggtaaa tattagtttt
    10381 tttgtcaaag acaactgacg actgatagga gagagagaga ctttctaaac catttgagat
    10441 ggcttggtga tgagtatttg cttctttctc taacgcattg ataaaatcaa tacgatgttc
    10501 catgacacgg gctccatagc cagctaattg ctcatctaga acagctagaa aagcggcatc
    10561 aatttgttgg gcacttttta aatagctatt acgctgttta agcacgtggt tataatggga
    10621 taactcagat aagtaaacag gcttaatttg accaaggtca atgtcgataa atttgcgacg
    10681 aaggctaggt gctcccttaa ctagttgcaa atcttctggt gcaaaaagca caaccatcat
    10741 cgttccaata taatcagata attttgcttg ctttagcgca ttaatctttg tcacacgtcc
    10801 tttatctgat agattgattt ctaaatcaac agtaccacta atacgctgaa tcttaccagt
    10861 aagagaaaca gttgagtgat caaaatgaat caattcttta tcagccctcg tgcgatggct
    10921 tctagtaagt gatagaaaat agattgcctc gagaaaattg gtttttcctt gagcattatt
    10981 accgataaag acatttaaac ccgacgaaaa tgaggcaagc aggtggtcat aattgcgata
    11041 atgttttaac tctaactctt taatccacat actagatacc tgggaagcgg acaggtgcag
    11101 ttggtttacc tttagttttc ttagttgctc ttagtgattt tttggtagta gattgacgat
    11161 ttttatgttt agaagaagtc tttttgttag cttggttcat ttgtttcact agagccgcta
    11221 cacgtgtctt ttcagctatt tcttcggcaa attgttcttt ttcttcttga ctaggctcaa
    11281 caatagtgat aattaagtct tggtcaggta gagaaatctt atcccctatt ctgatttttt
    11341 tgcctcgacg tttctcatct tcaccattaa atagaacggt tgtttcagcg agaaatccct
    11401 tgatagcgcc accactttgg ataattccta attctttaag aagggcttgt agggtgataa
    11461 attctgtaaa taatttgtat atcattgtca tctccatttt gtcattcaag tcattatagc
    11521 ataaaacaga aataaataat aatagctgaa taagagctat ttataaagta tcatactcgt
    11581 ccaaataact atttagtttt gaagttcttg ttaattagtt ttaacaaaag ctgtacttat
    11641 gtttatcatt tttctacgta taaatcctat tattaatatg tccctctagt aaagttgtat
    11701 aacacaaaac ttttatattt tggaacgttg acaattttca agatataaac ttaagttgct
    11761 ataaacaagg aaaaaagtaa agtaccaaca cattactttg aatgtgttgg tactttactt
    11821 ttttccaaag cttttcctag tttgataatg taatttttca agtcctcttt gacctctggg
    11881 tgttctaagg catagtcgat agatgttttc atgaatccaa atttatcccc aacatcgtaa
    11941 cgattgcctt taaattctcg tgcaaagaca cgctgagttt tattgagggt atcgatagca
    12001 tctgtgagtt gcacttcgtt acctgctcca ggggtctgtc tttccaaaat atcaaaaatt
    12061 tcaggggtta ggaggtaacg accaataata gccaaatcac taggcgcatc ttctggttgt
    12121 ggtttttcaa caaaggtgtc tacactgtaa aggcccttga cagccttgcc ttgaggagcg
    12181 ataaccccat agctagacac atcttcatga ggaactttca tcacagcgat agtggatgca
    12241 tgcgtcttgt catagtcctc catgagttgt ttggtgagag gtttagcgga tgcatttgta
    12301 atgtccatta agtcatctcc aagcatgacc acaaagggtt cattgcccac aaaggctttg
    12361 gcttgtaaga cagcatctcc cagtcctctt gggtggcttt gacggataaa atgaaggtta
    12421 atggcagtgg tttcatccac taatttcaac agttcatttt tccccttagc ttggaggttg
    12481 tattctaatt caaagtttga atcaaaatgg tcctcgatag agcgtttagc ttttccggtc
    12541 accacaagga tttcctcgat gccagatttt agcgcttctt cgacgataaa ctggatggtt
    12601 ggtttatcaa cgatgggcag catctctttg gcaagagctt tggtagcagg taaaaaacgt
    12661 gttcctagac ctgcagcagg aataatggct tttctgactt tggtcatcag taactcctca
    12721 ctttttataa tctatggctt agtgacattc gttttcagaa cggaattcat ttgacatcat
    12781 gttacgtttt catatgattt ttcgactatt tcatagtgct caatcatacc accaactgca
    12841 gttcttttaa agtaaaaatg aatattattg ttatagatga agacatggct agtctctatt
    12901 aaaaatatct ctactgtaaa ctttattttt aaca
//
LOCUS       DAUDYX010000053        12921 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.054, whole genome shotgun sequence.
ACCESSION   DAUDYX010000053 DAUDYX010000000
VERSION     DAUDYX010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12921
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.054"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(<1..1187)
                     /locus_tag="VHD02_001570"
     CDS             complement(<1..1187)
                     /locus_tag="VHD02_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEQ5498165.1"
                     /translation="MTTKYQTIISNIEQDIQKQRLKKGDKLPSIRVLSKVYHCSKDTV
                     QRALLELKYRHLIYAVPKSGYYVLGNVSIPDNVLNLSLEDYNNMAYEDFRLCLNEALS
                     AKDKYLFHYYHKTEGLEELREALLLYLAENSVYSNKDQLLITSGTQQALYILSQMPFP
                     NAGKTILLEKPTYHRMEAIVAQLGLPYQTISRHFNGLDLELLESLFQTGDIKFFYTIS
                     RFSHPLGLSYNTKEKEAIVRLAQRYQVYILEDDYLGDFVKLKEPPIHYYDTHHRIIYL
                     KSFSMSVFPALRIGALVLPSGLKPHFLTQKSLIDLDTNLLMQKALALYLENGMFQKNL
                     RFIKRYLKQRERQLALFLKQNCPDIHYQLTPTHLVIDYTTSDSYRNFTLDKSDRIIIT
                     GKKR"
     gene            1277..2143
                     /locus_tag="VHD02_001571"
     CDS             1277..2143
                     /locus_tag="VHD02_001571"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HEQ5498166.1"
                     /translation="MKRIIVANDLVGVGKVALSASIPLMASCLLEQVLLPTCLLSSHT
                     GGGQSPVKHTTSSILDVFLTDWDNKRIKFDGIFIGYFTTTRDGQKLLEWLSRKQLPLI
                     LDPIMADNGKLYQGFSQKHIEIMIKLTQKAELVLPNISEACLLTQTAYLAKNYDEEDI
                     RQVARKLAKLGPKQVVISGISFEEGSIGFAYYQSDSDQLTFHFGKQFPYHFYGTGDIA
                     SAIITAGFYYQLPLTQILDLVVSFLEKVLQNTLTLKRDLILGVAYESHLNYLTKSFKK
                     LLEEKNVISKIS"
     gene            2121..2678
                     /locus_tag="VHD02_001572"
     CDS             2121..2678
                     /locus_tag="VHD02_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ5498167.1"
                     /translation="MSSQKLANYIELSMYAALIFISVQVLRVPVGMQFIHLGNALVVI
                     AVLIYGSKKGALVASLGLGIFDVLNGYASVVWITILESLAICLILHLVYERAMQSSKK
                     PKAIITITVIAALSKLTINLIKYLLLAYIGTQLPLSVSFWVALGKIGGSFGTSFVTLV
                     AVPLLYPVIKNLKEKVTIGQAKRGF"
     gene            2812..4356
                     /locus_tag="VHD02_001573"
     CDS             2812..4356
                     /locus_tag="VHD02_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEQ5498168.1"
                     /translation="MSILEVKQLSHGFGDRAIFENVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGHLQPDEGKVEWSKYVTAGHLDQHTVLESGQTVRDVLRTAFDELFKTENRIN
                     EIYASMADDKADIAVLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMESDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNHLDAEHIEWLKRYLQHYENAFVLISHDI
                     SFLNDVINIVYHVENQSLVRYTGDYYQFQAVYEMKQSQLEAAYERQQKEIANLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPNFEFKQARTPSRFIFQTKNLVIGY
                     DYPLTKEPLNITFERNQKIAIVGANGIGKSTLLKSLLGVIEPLEGHIVTGDFLEVGYF
                     EQEVTGVNRQTPLEVVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQAKVR
                     FCLLMNRENNVLILDEPTNHLDIDAKNELKRALKAYKGSILMVCHEPDFYNGWVTDTW
                     DFSKLT"
     gene            4720..5943
                     /locus_tag="VHD02_001574"
     CDS             4720..5943
                     /locus_tag="VHD02_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEQ5498169.1"
                     /translation="MTNLTFYAKIGISEEEHDSFVKEHQQISVLQGSDWAKIKNQWQN
                     ERIGIYKEEKQVASLSLLIKLLPLGRSIIYIPRGPVMDYLDRDLVAFTMKTLKDYGKT
                     KKALFIKYDPAILLKQYALGQEEEEKPLALAAIKNLQEAGVHWTGLTMEIADSIQPRF
                     QANIYTQENLKMQFPKHTRRLIKDAKQRGVETYRASQSELHKFSKIVSLTEKRKNISL
                     RNEAYFQKLMTTYGDKAYLHLAKVNIPQKLDQYRQQLILINQDITRTQAHQKKRLKKL
                     EDQKASLERYITEFEGFTDQYPEEVVVAGILSISYGNVMEMLYAGMNDDFKKFYPQYL
                     LYPNVFQDAYQDGIIWANMGGVEGSLDDGLTKFKANFAPTIEEFIGEFNLPVSPLYHI
                     ANTMYKIRKQLKNKH"
     gene            complement(5985..7547)
                     /gene="guaA"
                     /locus_tag="VHD02_001575"
     CDS             complement(5985..7547)
                     /gene="guaA"
                     /locus_tag="VHD02_001575"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEQ5498170.1"
                     /translation="MTEISILNDVQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TAQELREINPIGIVLSGGPNSVYADNAFGIDPEIFELGIPILGICYGMQLITHKLGGK
                     VVPAGQAGNREYGQSTLHLRETSKLFSGTPQEQLVLMSHGDAVTEIPEGFHLVGDSND
                     CPYAAIENTEKNLYGIQFHPEVRHSVYGNDILQNFAISICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHGLLRKDEGDQVMGMLG
                     GKFGLNIIRVDASKRFLDLLADVEDPEKKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGIALGMPEEI
                     VWRQPFPGPGLAIRVMGAITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            7714..9147
                     /locus_tag="VHD02_001576"
     CDS             7714..9147
                     /locus_tag="VHD02_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498171.1"
                     /translation="MLRKKRILGLFRPVELIFLGLLLSLVVSYLAWTNSFATLHNILA
                     TVGIVERSKDQQPRYHIGQAIQVQKSGPYHQWIGTINKQVEDIAENYRVSYHYEVVFP
                     IGKVTVSLPEHNLKEPDKPRFKKGDIVKLSSLTKKPHIKVYQGQLATIKQVKKRYDYS
                     LGGYQYDINLKDNLRLDGISEQDFVKPYYIRFNKGNSPEQNNRLLRKAFAYAKQHPNS
                     VISFPKGQFHIGSLPSQKDYFELPSDTAIIGHQTEFIIHGKMLWFGFPTGPKAEQGVR
                     NLVLTGVHFKANDLKKGDHFMIMADHGTDWHIYDNKFTMVHKRNSHIFDLGSLQNSLF
                     EKNQFIGYAPELVQDQQLLSKAQGHDFFSEVIQFDAAVHHFAWDGGLLSNIAPNYEAF
                     NQTRHLCHNITVSQNQFLPYIDPTGCLRAYSGSIGQHSSKVGVIRVLNNVFTSSIVTK
                     AKLTSWFMEPIHFPPNSPVIVAGNIIN"
     gene            9230..9928
                     /locus_tag="VHD02_001577"
     CDS             9230..9928
                     /locus_tag="VHD02_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ5498172.1"
                     /translation="MLPAYIKIHDAIKKDIDLGIWPIGSRLPSERHLAEHFTVSRMTL
                     RQAITLLVEEGILERRIGSGTYVASHRVQEKMRGTTSFTEIIRSQGRQPSSKLLSYQK
                     QLASDTEVKELNLDKTDLVIRMERIRYADSVPLVYEIASIPEKFIKTVKRADITEHFF
                     HSLIANGYEIGKSKQTIYAKLASERVASYLEVAKGHAILALTQVSYFTDGKPFEYVRS
                     QYIGDRFEFYLENN"
     gene            10017..10358
                     /locus_tag="VHD02_001578"
     CDS             10017..10358
                     /locus_tag="VHD02_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEQ5498173.1"
                     /translation="MNIMEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEI
                     ADEFGVSRQAVYDNIKRTEKILETYEMKLHMYSDYVVRSEIFDDMIAHYPHDEYLQEK
                     ISILTSIDNRE"
     gene            10373..11935
                     /gene="ffh"
                     /locus_tag="VHD02_001579"
     CDS             10373..11935
                     /gene="ffh"
                     /locus_tag="VHD02_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEQ5498174.1"
                     /translation="MAFESLTQRLQDVFKHIRGKKKLSESDVQEVTKEIRLALLEADV
                     ALPVVKTFIKRVRERAIGHEIIDTLDPTQQILKIVNEELTSILGSETAEIDKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEENARPLMIAADIYRPAAIDQLKTLGQQINVPV
                     FDMGTDHSAVDIVRKGLEQARENRNDYVLIDTAGRLQIDEKLMEELRDVKALAQPDEI
                     LLVVDSMIGQEAANVAYEFNHQLSITGVVLTKIDGDTRGGAALSVREITGKPIKFTGI
                     GEKITDIETFHPDRMSSRILGMGDLLTLIEKASQEYDEKKSLELAEKMRENTFDFNDF
                     IEQLDQVQNMGPMEDLLKMIPGMAGNPALANIKVDENQIARKRAIVSSMTPAERENPD
                     LLNPSRRRRIAAGSGNSFVEVNKFIKDFNQAKSMMQGVMSGDMSKMMKDMGINPNNLP
                     KTMPAGMPDMSSLEGMMGQGGMPDLSGLGGDMDMSQLFGKGFKGKIGQFAMKQAMKRQ
                     ANKLKKAKKKRK"
     gene            complement(12070..12768)
                     /locus_tag="VHD02_001580"
     CDS             complement(12070..12768)
                     /locus_tag="VHD02_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ5498175.1"
                     /translation="MFSGHQLKTVRLSKGITQSELGRLLHVNKMTISNWEKGKNIPNE
                     KHLNALLHLFNVTSDYFDPNYRLLTPYNQLTISNKEKVIGYSERLLNHQIDKKSKDLI
                     DKPSQLYAYRVYESLSAGTGYSYFGDGNFDVVFYDEQLEYDFASWVFGDSMEPTYLNG
                     EVVLIKQNSFDYDGAIYAVEWDGQTYIKKVFREDEGLRLVSLNKKYSDKFAPYSEEPR
                     IIGKIIANFRPLEI"
BASE COUNT         4266 a         2169 c         2469 g         4017 t
ORIGIN      
        1 cgcttttttc cagttattat tattctatca gatttatcta atgtaaagtt tctataagag
       61 tctgatgtcg tgtaatcaat cactaaatga gtaggggtta gttgatagtg aatatcaggg
      121 caattttgtt ttaaaaagag agccaattga cgttcccgct gttttagata acgcttaata
      181 aagcgaagat ttttttgaaa catcccattt tctaaataaa gagctaatgc tttttgcatt
      241 agtaagttcg tgtctaaatc aatcaatgat ttttgggtta aaaaatgagg ttttaaacct
      301 gacggtaaaa ctagagctcc gatacggagt gcaggaaaaa cactcattga aaaagatttt
      361 agatagatga ttcggtgatg agtgtcataa taatgaattg gtggctcttt aagtttcaca
      421 aaatctccta aataatcatc ttctaaaata taaacctggt atcgttgtgc tagacgaaca
      481 atagcttcct tttccttagt gttataagaa agtcccagag gatgtgaaaa tcgtgaaatc
      541 gtgtaaaaaa atttaatatc gccagtttga aataaagatt ctaagagttc taaatcaagg
      601 ccattaaagt gtctagagat agtttgatag ggtaatccta actgagcaac aatagcttcc
      661 atccgatgat aagtaggctt ttcaaggaga attgttttgc ccgcattagg aaaaggcatt
      721 tgtgataaaa tataaagcgc ttgttgcgtc ccagaggtga ttaatagttg gtctttgtta
      781 ctgtaaacac tattttcagc taagtaaaga agcaaagctt cccttagttc ttctagtcct
      841 tcggttttgt gataataatg aaagaggtat ttgtctttag cacttaaagc ctcatttaag
      901 cagagtcgga aatcttcata agccatatta ttataatctt caagacttag attaagaaca
      961 ttatcgggta tgcttacgtt acccaaaaca taataaccac ttttaggaac agcataaatt
     1021 aagtgacgat attttaattc taaaagagcc cgctgaacag tatctttgct acaatgataa
     1081 accttactta ataccctaat tgaaggtagc ttgtcccctt tttttagtcg ctgtttctgg
     1141 atatcttgtt cgatattgga aatgattgtt tgatattttg ttgtcataaa ttgtccccat
     1201 acagttaagt gttataacta ttgtatctta tgcctctttt tcttacaata gttattatag
     1261 aaaagaggta atagctatga aacggattat tgtggcaaat gatctggttg gtgttggtaa
     1321 ggtagctttg tctgctagta ttcctttaat ggcttcttgt ttgttagagc aagttttatt
     1381 accaacttgc cttttatcaa gccatactgg cggaggacag agccctgtga agcatactac
     1441 atcaagtata ttagacgttt ttttaacaga ttgggacaac aaaagaatta agtttgacgg
     1501 catttttata ggttatttta cgacaacaag agatgggcaa aagctgctag aatggctttc
     1561 aagaaagcaa cttcccttaa ttttagatcc aattatggca gataatggaa aactttatca
     1621 aggcttcagt caaaaacata tagagattat gataaaactt actcaaaaag ctgagcttgt
     1681 tttacctaat attagtgagg cttgtctatt aacacaaaca gcttatttag caaaaaatta
     1741 tgatgaagaa gatattagac aggtggctag aaaattagct aagcttggac ctaaacaggt
     1801 tgtcattagc ggaatctctt ttgaggaggg cagcattggt tttgcttact atcagtcaga
     1861 ttcagatcaa ttaacattcc attttgggaa gcaattccct taccatttct atggtacagg
     1921 agatatagca tcagctatta taacagcagg attctattat cagctaccac taacacaaat
     1981 tctagatctt gttgtttcat ttcttgaaaa agtattgcaa aataccctta cattaaaaag
     2041 agatttaatc ttaggtgttg cttatgaatc tcatttaaat tacttaacta aatcgtttaa
     2101 aaagttgttg gaggaaaaaa atgtcatctc aaaaattagc taattatatt gaattgagta
     2161 tgtatgccgc tttaattttt atttcagtac aagtgttgcg tgtccctgtc ggtatgcagt
     2221 ttattcatct tggcaatgca ttagttgtta ttgcagtctt aatctatggt agtaaaaaag
     2281 gagctctagt tgctagtctt ggtttaggga tctttgatgt tttgaacggc tatgcttcag
     2341 tcgtttggat aactatctta gaatccttgg ctatttgttt aattttgcat ttggtatatg
     2401 aaagagctat gcaatctagt aaaaaaccaa aagccattat aacaataaca gttattgctg
     2461 cattgtcaaa attaactatt aaccttataa aatacttact gttagcttat ataggtactc
     2521 agctaccact ttcggtatct ttttgggtag ctcttggaaa aattggtggt agtttcggaa
     2581 catcttttgt gactttagtt gctgttcctc ttttgtatcc tgttatcaaa aacttaaaag
     2641 aaaaggttac aatcggacaa gcaaaaagag gtttttgata caagtacctt atcgataaaa
     2701 aagcaaggta cacttttagt caaatattga caaaaggtta caattttgcc ataatgaaat
     2761 aactataaag ccgttttgac ggcttttttg atgacagtta aggaagaaac gatgagtatt
     2821 ttagaagtta agcagttgag tcacggtttt ggggatcggg ctatttttga aaatgtgtca
     2881 tttcgcctct taaaaggcga acatattgga ctagttgggg caaatggtga aggaaaatca
     2941 acctttatga gtatagtcac aggacattta cagcctgacg aaggaaaagt agagtggtcg
     3001 aagtatgtca ctgcaggtca cctggatcaa catacagtgt tggaatcagg acaaaccgtt
     3061 cgtgatgtct tgcgaactgc ttttgatgag ttatttaaga ccgagaatcg tattaatgag
     3121 atttacgcgt caatggcaga tgataaagct gatattgctg ttttgatgga agaagtaggt
     3181 gagcttcaag atcgtttaga aagtcgtgat ttctatactt tggatgctaa gattgatgaa
     3241 gtagcgcgtg cgcttggtgt tatggatttt ggaatggagt cagatgttac atccttatca
     3301 ggtgggcaac gaacaaaggt tttattagcc aaattactat tagaaaaacc tgatattctg
     3361 ctattagatg aaccaactaa ccatttggat gctgagcata ttgaatggtt aaaacgctat
     3421 ttacaacatt atgaaaatgc ttttgtgttg atttcgcatg atatttcttt cttaaatgat
     3481 gtgattaata ttgtttatca tgttgaaaat caaagtttag ttcgctatac tggggattat
     3541 taccaatttc aagctgttta tgagatgaaa caatctcaac ttgaagcagc ctatgaacgt
     3601 caacaaaaag agattgctaa cttgcaggat tttgtcaacc gaaataaggc tcgggtagcg
     3661 acacgtaaca tggcaatgtc tcgccaaaaa aaacttgata agatggatat aattgaactt
     3721 caagctgaga aaccaaaacc aaattttgaa tttaagcaag ctagaactcc cagtcgattc
     3781 atttttcaaa caaaaaatct tgtgattggt tatgattacc cattgaccaa agaaccctta
     3841 aatataacgt ttgaaagaaa ccaaaaaatt gctattgttg gggccaacgg tattggaaaa
     3901 tctactttgc taaaaagttt attaggtgtt atcgagcctt tagaaggtca tattgtcaca
     3961 ggggattttt tagaagttgg ctactttgaa caagaagtga caggtgttaa ccgacaaact
     4021 ccgctagaag tagtttggga tgcttttcct gccttaaatc aggcagaagt tcgagcggca
     4081 ctagctcgtt gcggactaac atcaaaacat attgaaagtc aaattcaagt actttcgggt
     4141 ggtgaacaag caaaagttcg tttttgtttg ttgatgaatc gtgaaaataa cgtgcttatt
     4201 ttagacgaac caacaaatca tcttgatatt gatgctaaaa atgagctcaa acgtgcttta
     4261 aaagcatata agggttctat tttaatggtt tgtcatgaac ctgatttcta caatgggtgg
     4321 gtaaccgata cttgggattt tagtaagtta acctaaacta ttccttaatt ttttacacag
     4381 aaattactaa aatcagtaaa gatatagaat aaatactgct agatatgtga tggtttaggt
     4441 aactgatcac caaccaaaaa agatgtgact acatcttttt ttatctaatg gaatggtgta
     4501 aagtcgttta ataatagctt attttttgat gatcagttgt tcatgaaatt gttggtgatt
     4561 aacaagtcat ttaagataca aacgttatta ctaaaaagtg attcaaatag tttccctatt
     4621 tttatgtttt tttaacattt attacgaata aatagataaa gacagtgaag atgattagta
     4681 agtacatctg ctaagattac gaatagatgg ggacaataaa tgacgaattt aacgttttat
     4741 gcaaaaatcg gtatttcaga agaagagcat gactcttttg ttaaagaaca tcagcaaatt
     4801 agcgttttac aaggtagtga ttgggcaaaa atcaaaaatc aatggcagaa tgagcgaatt
     4861 ggtatctata aagaggaaaa gcaggttgcc tctttatcac ttttgattaa gctattgcca
     4921 cttggaagaa gcattattta tattccaaga gggccagtca tggattatct tgaccgtgat
     4981 ttggtggcat ttaccatgaa aacactaaag gattatggta aaactaaaaa ggccctcttt
     5041 atcaaatatg atccagctat cctgttaaaa caatacgcac tgggacagga agaagaagaa
     5101 aaacctttag ctttagcagc tattaagaat ctccaagaag ctggtgttca ttggactggt
     5161 ttaacaatgg agattgcaga tagtatccaa cctcgtttcc aagctaatat ttacactcaa
     5221 gaaaacctta agatgcaatt tcctaagcat accagacgtt taataaaaga tgctaagcag
     5281 cgtggtgtag aaacatatcg tgccagtcaa tcagaacttc acaaattttc caagattgtc
     5341 tccttaacag aaaaacgtaa aaatatttct ttgcgtaacg aagcttactt tcaaaagttg
     5401 atgactactt atggggataa ggcctactta catctagcaa aagtgaatat tcctcaaaaa
     5461 ctagatcaat accgccagca attaattctt attaaccaag atattactcg cacccaagct
     5521 catcaaaaga agcgtttaaa aaaattagaa gatcaaaaag cttctttaga acgttatata
     5581 actgaatttg aaggctttac agaccaatat cctgaggaag ttgttgtagc aggtatatta
     5641 tctatttctt atggaaatgt tatggaaatg ctttatgctg ggatgaatga tgattttaag
     5701 aagttttatc ctcagtatct gctgtatcct aatgtttttc aggatgctta tcaagatggt
     5761 attatttggg ctaacatggg aggagtagaa ggctcgcttg atgatggact taccaaattt
     5821 aaggccaact ttgctccgac aatagaagaa tttataggag aatttaatct ccctgtcagc
     5881 ccactttatc atattgctaa taccatgtac aaaatacgaa aacagttaaa gaataaacat
     5941 taaaagactc gccaaaatga gcgagtcttt taatgtttat tctgttattc ccattcaact
     6001 gttgcgggtg gtttacttgt gatgtcgtag acgatacggt taacgtggtc aacttcattt
     6061 acgatacgtg ttgagatttt tttcaagaca tcccaaggaa gttgagcaaa gtcagctgtc
     6121 ataccatcaa tagacgtaat agcacgaatg gcgatggtat aatcataagt acgaccatct
     6181 cccatgacgc ctacagaacg gacacctgtg ttaactgtaa agtattgcca cacgtcacga
     6241 tcaagtccag ccttagcaat ttcttcacga aggatagcgt ctgattcgcg aacggtttca
     6301 agtttttctt cagtaattgc tcccatgaca cggatagcaa gtccaggacc tggaaatggt
     6361 tggcgccaaa caatttcttc aggcattcca agagcgattc caagcgctcg aacttcatct
     6421 ttgaaaagag tgtttaaggg ctcaatcaat tcaaactgca tgtcttcggg gagaccaccc
     6481 acattgtgat gtgatttgat ggtttgagca gtttctgttc ctgactcaat gatatcagta
     6541 taaagtgttc cttgggcaag gaagtcaaca ccttttaatt tgctggcttc atcatcaaaa
     6601 acatagacaa attcattacc aataatttta cgttttttct caggatcttc aacgtctgca
     6661 agaaggtcta agaaacgttt tgaagcatcc acacggataa tatttaggcc aaatttgccc
     6721 ccaagcattc ccataacttg atcgccctcg tctttacgaa gaagaccgtg atcaacgaaa
     6781 atacaagtta attggtcacc gatagctttt tgaagtagaa caccaacaac tgaagaatca
     6841 actccaccag aaagacctag aagaacttta cgatcgccta cagtttcacg aattttagca
     6901 atttccatgt caataaaatt atccattgac caatcaccac gcgcgccaca aattgatata
     6961 gcaaagtttt gaagaatgtc atttccataa acagagtgtc ttacttctgg gtggaactga
     7021 ataccgtaaa ggtttttctc agtattttca atagctgcat agggacagtc atttgagtct
     7081 ccaacaaggt ggaaaccttc tggaatttca gtaacagcat caccatggct cattaaaacg
     7141 agttgttctt gaggtgtgcc tgaaaataat tttgacgttt cacgaagatg aagggttgac
     7201 tgaccgtatt cacgattacc agcttgtcca gcaggaacaa ctttaccacc taatttatgg
     7261 gtgattaatt gcataccgta acagatacca agaatcggaa tccctagttc aaagatttca
     7321 gggtcaatgc caaaggcgtt atcagcgtaa acagagttag gccctcctga taaaacgata
     7381 cctatgggat tgatctcacg aagttcttga gcggtgattt tatggctttt tagttcggag
     7441 aaaacaccaa actctcgaat acgtctagca ataagctgat tgtactggct accataatca
     7501 agaacgataa ttttttgaac atcattcaaa attgaaattt cagtcattat aatcctttca
     7561 ttctatacca accgtcaggc tgatagttgt caacttattg tagcatattt tcatcgaaaa
     7621 gacattacaa tttctctagc tcgttggaaa aaaacgtaat cgttatgtta caatagaggc
     7681 tatgaaaggg aattaagaca acagggaggg cctatgctaa gaaaaaagag aattttgggt
     7741 ttatttcgac ctgttgaatt aattttccta ggattattgt tgagtttagt tgtttcgtac
     7801 cttgcgtgga ccaatagttt tgcaacactt cacaatatat tagcaactgt cggaatagtg
     7861 gaacgaagca aagatcaaca gcctcgctat cacattggac aagctattca agtacaaaaa
     7921 agtggtccat atcatcaatg gattggaact attaataaac aggtagaaga tatagccgaa
     7981 aattaccgag tgagttatca ttatgaagtg gtatttccaa taggaaaagt cactgtttct
     8041 ttgccagaac ataacctgaa agagcctgat aaaccgaggt ttaaaaaagg agatatcgtt
     8101 aaattatctt cattaactaa aaagccacat ataaaagtat atcaaggtca attagcgact
     8161 attaaacaag ttaaaaaacg ctatgactat tcgttaggag gatatcagta cgatattaat
     8221 ctgaaagata atctaagatt agatggaatt tcagagcaag attttgttaa accttattat
     8281 attaggttca ataaaggaaa ttcccctgag caaaacaatc gtcttttgcg aaaagctttc
     8341 gcttatgcaa agcagcatcc aaatagcgtt atatcttttc caaagggaca atttcacatt
     8401 ggctctttgc cttcacaaaa agattatttt gagcttccat ctgatacagc tattattggt
     8461 catcagacag agttcattat tcacggtaaa atgttgtggt ttggattccc tacaggacca
     8521 aaggctgaac aaggtgttcg taatctcgtg ttgactggag tgcatttcaa agcaaatgat
     8581 ttgaaaaaag gagaccactt tatgattatg gctgatcatg gtactgattg gcatatttac
     8641 gataacaaat ttactatggt tcataagcgt aatagtcata tttttgattt aggatctcta
     8701 caaaattcat tgtttgagaa aaaccaattt attggctatg cgccagaatt agtacaagac
     8761 caacagctgc tatcaaaggc tcaagggcat gatttttttt cagaagtcat tcagtttgat
     8821 gctgctgttc atcattttgc atgggatgga ggtctactta gtaatattgc tccaaactat
     8881 gaagcattta accaaactcg acatctatgt cacaatatta ctgtaagcca aaatcaattt
     8941 ttaccttata tagatccgac tggttgcctg agagcctata gtggttctat tggtcagcat
     9001 tcctcaaaag taggagttat tagggtttta aataatgttt ttacctcatc cattgttact
     9061 aaagcgaagc tcactagttg gtttatggaa cctattcatt ttccaccaaa ttcaccagtt
     9121 attgtcgcag gtaatatcat taattgatgc caaatacctc aatatttaca attcaatttt
     9181 catcaaaaat atgataaaat agatgaataa ccgacttgag gagtgtggca tgttaccagc
     9241 ttatataaaa attcatgatg caattaaaaa agacattgat ctgggcatct ggccaattgg
     9301 tagtcgctta ccaagtgaac gacatttagc ggaacatttc accgttagtc gaatgactct
     9361 acggcaggct attacattac tagtagaaga agggattctt gaaaggcgga taggaagtgg
     9421 tacttatgtt gccagtcatc gtgttcaaga aaaaatgcga ggaacaacaa gtttcacaga
     9481 aattatacgc tcccaaggac gacaaccctc ctctaaatta ttatcctatc aaaagcaatt
     9541 agctagtgat accgaagtta aagaattgaa cttagacaaa acagacttgg tcattcgaat
     9601 ggaacgtatc cgctatgcag acagtgtccc tttggtgtac gaaattgctt caatcccaga
     9661 aaaatttata aaaacggtta aacgagctga tattacagaa cacttttttc actcattgat
     9721 agcaaatggt tacgagattg gtaaaagtaa acagacaatc tacgctaagc tagcaagtga
     9781 gcgtgtagcc tcctacttgg aagttgctaa aggacatgcc attttggctc taactcaggt
     9841 gtcttacttt acagacggca aaccttttga gtatgtccgc agtcagtata tcggtgaccg
     9901 ttttgaattt tacttagaaa ataattagta aagaagtcat ttatgacttc cttttttaca
     9961 cgaacagcat aaggcaatta atttgctact gttaaaaaca atcatagtat gataaaatga
    10021 acattatgga aattgaaaaa acaaacagga tgaatgccct ttttgaattt tatgcagctt
    10081 tgctaacaga taaacaaatg aattatattg agctgtatta tgccgatgat tatagcttag
    10141 ctgagattgc tgatgaattt ggtgtcagtc gtcaggctgt ctatgataat attaagcgta
    10201 cggaaaaaat tttagagact tatgagatga aacttcatat gtattcggat tatgttgttc
    10261 gaagtgaaat ctttgacgac atgattgctc actaccctca tgatgagtat ttgcaagaaa
    10321 aaatttccat tctaaccagt attgacaata gagagtaaga ggagttttga ctatggcttt
    10381 tgaaagctta acccaacgtt tgcaagatgt gtttaaacac attcgcggta aaaagaaatt
    10441 atcagaatca gatgttcaag aagtgacaaa agagattcgt ctagctcttt tagaagccga
    10501 tgtcgcttta ccagttgtta aaacctttat taagcgcgta cgtgagcgcg ctattggcca
    10561 tgagattatt gatactcttg atccaacaca acaaattctt aagattgtta atgaagaatt
    10621 aacgagtatt ttaggctcag aaaccgcgga gattgataaa tctccaaaaa ttccaactat
    10681 tatcatgatg gtcggtttgc aaggggctgg taaaaccact tttgcaggaa aactcgctaa
    10741 taaattaatt aaagaagaaa acgcgcgacc actgatgatt gctgccgata tttaccgtcc
    10801 agcggccatt gatcagttaa aaacactggg gcaacaaatc aatgtacctg tttttgatat
    10861 ggggacagac cactcagcag tcgatattgt cagaaaaggt ttggaacaag ctcgtgaaaa
    10921 ccgcaatgac tatgtcttga ttgatactgc gggtcgcttg caaattgatg aaaaactgat
    10981 ggaagagctg cgcgatgtca aggctttagc acaaccagat gaaattcttt tggttgtaga
    11041 tagcatgatt ggtcaagaag ctgccaatgt agcctacgag tttaatcatc agttgagcat
    11101 cacaggggtt gtgttaacaa agattgatgg tgatacccgt ggaggtgcag cactatctgt
    11161 ccgtgaaatc actggaaaac caattaaatt tacaggtatt ggtgaaaaaa tcacagatat
    11221 tgaaacattt cacccagacc gcatgtccag tcggattttg ggaatgggtg acttactcac
    11281 tcttattgaa aaagctagcc aggaatatga tgagaaaaaa tccttagagt tagctgaaaa
    11341 aatgcgtgag aacacctttg atttcaatga tttcattgaa cagttagatc aagtgcaaaa
    11401 tatggggcct atggaagacc tcttgaaaat gattccagga atggctggta acccagcttt
    11461 ggccaatatc aaagttgacg aaaaccaaat tgctcgcaaa cgtgctattg tctcttcgat
    11521 gacccccgct gaacgtgaaa accctgactt actaaacccg agtcgtcgcc gtcgtattgc
    11581 tgctggttcc gggaacagct ttgtggaagt taataaattt atcaaagact ttaaccaagc
    11641 caaaagcatg atgcaagggg ttatgtcggg cgatatgtct aagatgatga aagatatggg
    11701 cattaatccc aataatcttc ctaaaaccat gcctgctggt atgccagata tgtcttcctt
    11761 agaagggatg atgggacaag gtggtatgcc cgatttatct ggccttggtg gtgatatgga
    11821 tatgagtcaa ctatttggta aagggttcaa ggggaaaatt ggacaatttg ccatgaaaca
    11881 ggccatgaaa cgtcaagcta acaaactaaa aaaagcaaag aaaaaacgaa aataaaagag
    11941 acggaaattt tcgtctcttt ttgatgtgaa attcatttat cttgtataca agattattaa
    12001 aattatggaa gaccgcgtta catgcatgat gagttcattt aaaatttgaa ataacctaat
    12061 caatctaact taaatttcta agggcctaaa attagcgata attttgccaa taatgcgagg
    12121 ttcttcgcta tagggagcaa acttatcaga atatttttta tttaaggaca ctagacgtaa
    12181 tccctcatct tcacgaaata cctttttgat atatgtttgc ccatcccatt cgactgcata
    12241 aattgctcca tcgtaatcaa aactattttg ttttataaga acaacttcgc catttaaata
    12301 agttggctcc atagaatctc caaaaaccca agacgcaaaa tcgtattcta attgttcatc
    12361 gtaaaagaca acatcaaagt taccatcacc aaaataggag taaccagtac cagcagataa
    12421 actttcatag acccgataag catataattg tgatggttta tctatgagat ctttagattt
    12481 tttgtctatt tgatgattta acaatcgctc tgaatagcca attacttttt ctttattaga
    12541 tattgtcagc tggttataag gcgttagcaa tctatagttt gggtcgaaat aatcagatgt
    12601 cacattgaat agatgcaata aggcatttaa atgtttttca tttggtatat tcttaccttt
    12661 ttcccaatta gatattgtca ttttattgac atgcaacaat cttcccaatt ctgattgagt
    12721 tattcccttt gataatcgta ctgtttttag ttgatgtcca gaaaacattt ggtagctcct
    12781 ttagtaaagt tataacttta ctaatagtat caaaaaaata aaacgaaagc aagtgtaaca
    12841 tttcctacaa ttaattcatg tgatagcaat actgtcaaaa aggggaaaca cctaaaacat
    12901 agcgactaga ttcgtgtact a
//
LOCUS       DAUDYX010000054        12066 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.055, whole genome shotgun sequence.
ACCESSION   DAUDYX010000054 DAUDYX010000000
VERSION     DAUDYX010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12066
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.055"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(<1..2259)
                     /locus_tag="VHD02_001581"
     CDS             complement(<1..2259)
                     /locus_tag="VHD02_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ5498176.1"
                     /translation="MTDDKFDLLPLLDYIDPSKLSYQDWVNVGFALKHEGYTAMDWDI
                     WSQSDSRYKKGECFAKWDSFQGNGLGTVTGATITQLAKENGWTSDYRTSDEAHELDWD
                     STIDRDYKIIDKNWIEAKEIREPTNWSPVHDLITYINTLFESTDKVGYVTETYPIEID
                     KGIIYKPTKGAYDRTAGQLIQELQKNPNDIGAVLGDYKEEAGAWIRFNPLDGNGVKND
                     NVTDFRYALVESDSMELGKQYALFKELELPIATLVHSGKKSLHAIVKVDAKDYQEYRK
                     RVDYIYQICKKNGLDIDTQNRNPSRLSRMPGIMRNGHKQFLIDTNIGKTNYEEWYQWI
                     EDLNDDLPEPETLADEWDNLPELAPELIKGVLRQGHKMLIAGPSKAGKSFALIELSIA
                     LAEGQKWLGWQCEQGKVLYVNLELDRPSALHRFKDVYDAMGLPPANVANIDIWNLRGK
                     TVPMDKLAPKLIRRSLKKNYQAVIIDPIYKVLTGDENSADQMAHFTNQFDKVATELGC
                     SVIYCHHHSKGSQGGKKSMDRASGSGVFARDPDALIDLVELELTEDLIKARSEKAAAK
                     IYQQALQEKALGYYQQEVTLDDLESRYQMQQHFDKAIKDIMVKQPYLEAVKKAQHEVE
                     IATAWRVDGTLREFAKFQPVNMWFSYPKHEVDTTGVLADIQLEDSKPLWKKAKEARKS
                     KEENLKERNQKLETAYSALFDGSTPVTVQEIREYLDLKSNKSVENYIKEHDGYDVKKG
                     VVFQISDNKETEK"
     gene            complement(2249..2878)
                     /locus_tag="VHD02_001582"
     CDS             complement(2249..2878)
                     /locus_tag="VHD02_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498177.1"
                     /translation="MGEYCHLKELDGLRFGSLTVINRNRNNSKGGNARWNCLCDCGNK
                     TVVIGSKLRSGYTKSCGCARKNDNAKGYSSTRLYRIWKGMMNRCYNHKNDNYKYYGGK
                     GISICDEWLTFINFRTWSLSNGYKESLTIDRINPKGNYTPLNCRWVSMKMQQNNKTNN
                     RYLSYLGQEYTIAEFSEKLNVTYWTVINQLKLGWSVERIVEEARMKNDR"
     gene            complement(2888..4471)
                     /locus_tag="VHD02_001583"
     CDS             complement(2888..4471)
                     /locus_tag="VHD02_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEQ5498178.1"
                     /translation="MQLRPYQEEARSAVQHEWEDGKKRTLLVLPTGCGKTIVFSKIIE
                     DRVRLGERVLVLAHRSELLEQASDKLMTATGLGTALEKAENTSLGSWFRVVVGSVQTM
                     QREKRLSKFPTNHFDTIIIDEAHHAISDGYQRVLQHFDSSNVLGVTATPDRGDKQNLG
                     KYFDSLAYEYSLVDAIKSGYLSKITAVTIPLTLDLSTVSQQAGDFKASEIGTALDPYL
                     EQIADEMVKQCANRKTVVFLPLVKTSQKFRDILNQKGFKAAEVNGESKDRAEVLEDFD
                     NDKYNVLCNSMLLTEGWDCPTVDCVVVLRPTKVRALYSQMVGRGTRLAPGKENLLLLD
                     FLWHTERHELCRPAHLIAKTPEVAQKMVDNMTEETEVTFELLEAEEVASKDVVAEREE
                     ALAKQLAEMRKRKRALVDPLQFEMSINAEDLVDYVPEFGWEMAPVSDKQKNALEKFGI
                     FTDDIGNAGKATKLLERLAKRRETGLTTPKQIRMLERYGFKNVGMWKFEVASNMINRI
                     AASGWRLPKGINAREYQPE"
     gene            complement(4471..5106)
                     /locus_tag="VHD02_001584"
     CDS             complement(4471..5106)
                     /locus_tag="VHD02_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498179.1"
                     /translation="MTEYNNNFDRELGWDDEITQDSVGFVQLVPGDYQFTVTNMERAR
                     HTPNPKSPGKLPACNKAVVTIQIETSEGIAQLTHNLFLHTTTEGMLSAFFGAIGQKKH
                     GEPLKMNWNTVIGAKGVARINKRKGTGDYADREYDNIKSMIYADEVDWTKVLNKDVAQ
                     PQQMTHQQSAQPTYQGQQAQGTNFAQQPATQSYQQGQMQTPQQPQGGWGGF"
     gene            complement(5128..5862)
                     /locus_tag="VHD02_001585"
     CDS             complement(5128..5862)
                     /locus_tag="VHD02_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498180.1"
                     /translation="MTENIKEALEYAVNTAHEKPKTILGSDGKEYYDRNKYSLVELKT
                     KYYPKTLNLNTLDSLIDYLKSDMNNINSKRLMVIVEGPREVIVCEEDDDDLNRNVLVT
                     VEAIIPNVQFGRYDSPSDFNISLQSLFVNADDRNTVIEFASALKIENGSEIVDDGIGQ
                     TATIKQGVASLAKAKAPNPVTLRPYRTFSEVEQPSSEFIFRINQLANMALFEADGGKW
                     RLDAINNIANYLKEELADQTNITILA"
     gene            complement(5864..6286)
                     /locus_tag="VHD02_001586"
     CDS             complement(5864..6286)
                     /locus_tag="VHD02_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498181.1"
                     /translation="MNKTIKLDLSAIGEGGLQEKVDKELEKVFDNILDPNTETKVARK
                     VTITLTMKPDDARQTVSTAMEVKSTLAPQTGVATTVLVGQKDGKVYANELKSAMPGQT
                     YFDDEAVLRTDIGEPIDHLEKGINEDVIDFNKQKKVGN"
     gene            complement(6326..7417)
                     /locus_tag="VHD02_001587"
     CDS             complement(6326..7417)
                     /locus_tag="VHD02_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEQ5498182.1"
                     /translation="MQITRGKRARAQRVIIYGPEGIGKSSFAANFPEPLFIDTEGSTD
                     NMDVARMDKPTSYTMLKNQIAWIKANPTCCKTLVIDTIDWAESLIVDDVCAQHGKKGI
                     EDFGWGNGYTYTKEEVGRFLNMLQELIELGINIVLTAHAQMRKFEQPDEMGAYDRWEL
                     KLGKKTSSQTAPLVKEWADMVLFANYKTVVMTSESKKKKATGGQRVLYTQHHPAWDAK
                     NRHGLPDEMPLDYAVIAHLFAQVPTQPVPQTPPVQETPASQTAHESVHEQAEKAPEQP
                     PMQPTSAPVAYPPSMPKALTDLMSAEQVTPDELVAVANIRGHFPPMTPIENFPSDYWN
                     MIVANWLATLEVIKTQVRTVEPPFTVEGA"
     gene            complement(7432..8751)
                     /locus_tag="VHD02_001588"
     CDS             complement(7432..8751)
                     /locus_tag="VHD02_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ5498183.1"
                     /translation="MSITINKLEIENVKRIKAVKIEPSATGLTIIGGNNNQGKTSVLD
                     AIAWALGGNKYKPSQAMREGSQVPPTLKITMSNGLIVERKGKNASLKVIDPNGQKGGQ
                     QLLDSFVEELAINLPKFMDSTPKEKADVLLEIIGVGDQLAELELKEKEIYNQRHAIGV
                     IADQKEKFAKEMTYYPDAPKQLVSISELIQQHQAILAKNGENAQKRQNVERIRYDYNQ
                     SILEVDRLRKLLADAEAKTNKLSEDLKIANTDAMDLHDESTAEIEANIADIDEVNRKV
                     RANFDKDKAEEDAKQQREQYNILTNDIESIRQQKRDLLINADLPLEGLSVDDGKLLYL
                     GQEWDNMSGSQQLIVATAIVRKLKPDCGFVLIDKLEQMDNITLEQFGKRLEQEGLQAI
                     ATRVSIGEECAIIIEDGYSVENKAHNFKTAEGGNFAETVAPTWKGEF"
     gene            complement(8751..8978)
                     /locus_tag="VHD02_001589"
     CDS             complement(8751..8978)
                     /locus_tag="VHD02_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498184.1"
                     /translation="MKQQLINDNEFLRDENRRLNNELAEHYFVVTAKANLLDVIIASD
                     FILKSTLDKCIAELDEIDQIALLKAWNKGEL"
     gene            complement(9102..9239)
                     /locus_tag="VHD02_001590"
     CDS             complement(9102..9239)
                     /locus_tag="VHD02_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498185.1"
                     /translation="MKNKKEQWTPKVHCFRKDGSQCEPENITVPISFAGYYQILLELG
                     V"
     gene            complement(9308..9538)
                     /locus_tag="VHD02_001591"
     CDS             complement(9308..9538)
                     /locus_tag="VHD02_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003131906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ5498186.1"
                     /translation="MTINLKRLKAERIASGMTQCEVAQSMGWKTRTPYAKRENGIVSI
                     GADELAKITLIFGLPIEKIAIFFDKDVPVMER"
     gene            9722..10483
                     /locus_tag="VHD02_001592"
     CDS             9722..10483
                     /locus_tag="VHD02_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ5498187.1"
                     /translation="MRTNSEIISLIQSKVEERKMSMSELARNVGIAKSTMSRYFNKTR
                     EFPLNRADDFAKALNISTEFLLGIDLNSEVDGSELLGIYRELEEQRRVIVLDTAKEQL
                     EEQTKIINFSDKKTDIEDIFEVKGTTFAAAASGFGRGFEADDYDTYTVYTDEEPPHYD
                     YAIGIRGDSMLPKYEQGDMLYIVDKGMSTYSGQLCIVVHNGNTYFKKVYTEHDGLRLV
                     SLNKKYSDIFIGYPPAEDTYIKIYDVVGSFTPIEF"
     gene            10510..10875
                     /locus_tag="VHD02_001593"
     CDS             10510..10875
                     /locus_tag="VHD02_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498188.1"
                     /translation="MTLKQLLKSKNLGEYFEAKNNAEKMNEIENRGLKQVMKNSVRQT
                     QLNSEIKQVIKKEKEAVRCPKCKSTNVGFMQQGKKTFSVKKAVAGTLLIGGTVMGFLG
                     EKGKKQWHCNECSCIFETK"
     gene            10999..>12066
                     /locus_tag="VHD02_001594"
     CDS             10999..>12066
                     /locus_tag="VHD02_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEQ5498189.1"
                     /translation="MRKVAIYSRVSTINQAEEGYSIQGQIEALTKYCEAMEWKIYKNY
                     SDAGFSGGKLERPAITELIEDGKNNKFDTILVYKLDRLSRNVKDTLYLVKDVFTANNI
                     HFVSLKENIDTSSAMGNLFLTLLSAIAEFEREQIKERMQFGVMNRAKSGKTTAWKTPP
                     YGYRYNKDEKTLSVNELEAANVRQMFDMIISGCSIMSITNYARDNFVGNTWTHVKVKR
                     ILENETYKGLVKYREQTFSGDHQAIIDEKTYNKAQIALAHRTDTKTNTRPFQGKYMLS
                     HIAKCGYCGAPLKVCTGRAKNDGTRRQTYVCVNKTESLARRSVNNYNNQKICNTGRYE
                     KKHIEKYVIDVLYKLQHDKEYL"
BASE COUNT         3443 a         2405 c         2142 g         4076 t
ORIGIN      
        1 cttttccgtt tctttgttat cagaaatttg gaacacaaca ccttttttga catcgtaacc
       61 atcgtgttcc ttgatataat tttcaacgct tttattggat tttaaatcca agtattcacg
      121 aatttcttgc actgtaaccg gtgttgaacc atcaaataag gcgctataag ctgtctcaag
      181 tttttggttc cgttccttga gattctcttc tttacttttc ctagcttctt tagctttttt
      241 ccaaagaggt ttactatctt ccaactgaat atcagccaac acgccagttg tatccacctc
      301 gtgcttggga taactaaacc acatattaac tggctgaaac ttcgcaaact cacgtagcgt
      361 tccatcaact cgccacgctg tggcaatttc tacctcatgc tgggcttttt tgacggcctc
      421 aagatatggc tgtttaacca tgatatcctt gatagctttg tcaaaatgtt gttgcatctg
      481 ataacgactt tctaggtcgt ctagcgttac ttcctgttga tagtatccta gagctttttc
      541 ctgcaacgct tgttggtaaa tcttagcagc tgctttttct gagcgtgcct tgataaggtc
      601 ttcagtcaac tcaagctcca ctaagtcaat caatgcatca gggtcacgag caaatactcc
      661 agaaccactt gccctgtcca tagatttttt gccaccttga cttcctttag aatggtggtg
      721 gcaataaatt acgctacatc caagttcagt ggccacctta tcaaattgat ttgtaaaatg
      781 ggccatctga tcagcactgt tttcatcgcc agtgagaact ttataaatag ggtcgataat
      841 aacagcttgg taatttttct tcagtgagcg tctgataagt ttaggtgcta atttatccat
      901 tggtactgtt tttccacgga ggttccaaat gtcaatgttt gccacattag cgggaggtaa
      961 tcccatcgca tcataaacat ctttaaagcg gtgcaaagct gatggtctgt ctagctccaa
     1021 attgacatag aggactttcc cttgttcaca ttgccaacct aaccatttct gtccctcagc
     1081 caaagcaata gataactcaa tcaatgcaaa tgacttaccg gcttttgatg gtcctgcaat
     1141 tagcatttta tgtccttggc gtaacactcc tttaattaat tcaggagcca gctctggcaa
     1201 attatcccat tcatcagcta gcgtttctgg ctctggtaaa tcatcattta aatcttcaat
     1261 ccattgatac cattcttcat aattggtttt accaatattt gtatcaatca aaaattgctt
     1321 gtgaccattt cgcataatac ctggcattcg tgatagacga cttggattac gattctgtgt
     1381 gtcaatatca aggccgtttt tcttacagat ttgatagatg taatcaaccc gtttgcggta
     1441 ctcctgataa tctttagcat ctactttgac aatagcgtgt aatgattttt taccgctatg
     1501 gaccaatgtc gctattggca attctagttc tttaaataaa gcatactgct taccaagttc
     1561 catactatcg gattcaacca aggcatacct aaagtctgtt acattgtcgt ttttgacacc
     1621 attgccatct aaagggttaa atcgtatcca tgcaccagct tcttctttat agtcaccgag
     1681 gacagcacca atgtcattag gatttttttg taattcttgg ataagctgac cagctgtcct
     1741 gtcataggct ccttttgttg gtttatagat aatgccctta tctatttcaa tcgggtatgt
     1801 ttctgttaca tatccgactt tgtcagttga ttcaaaaaga gtgttgatgt aagtaatcaa
     1861 atcgtgaacg ggtgaccagt tagtaggttc tctaatttct tttgcttcga tccagttctt
     1921 gtcaataatt ttatagtcac gatcaatagt gctatcccaa tcaagctcgt gggcttcatc
     1981 actcgtacgg tagtcagatg tccacccgtt ttctttcgct aactgtgtga tagttgcgcc
     2041 agtgacagtc ccaagtccat taccttggaa actatcccat ttagcgaaac attcaccctt
     2101 tttgtatcta ctgtcagact gtgaccaaat gtcccaatcc atagcagtat atccttcgtg
     2161 ttttagggca aaaccaacat tcacccagtc ttgatatgat agttttgatg gatcaatata
     2221 atcaagtaaa ggtaataaat caaatttatc atctgtcatt tttcattctc gcttcttcta
     2281 ctattctttc tacactccaa cctaatttca attgatttat tacagtccaa taggtaacat
     2341 ttaacttctc tgaaaattca gcaatagtat attcttgacc taaatatgat aaatacctat
     2401 tattcgtttt attgttttgc tgcattttca tacttaccca tctgcaattt agtggagtat
     2461 agttaccttt tggatttatt ctatcaatgg ttaatgattc tttatatcca ttagagagcg
     2521 accatgttct aaaattaata aaagttagcc attcatcaca gattgaaata ccttttccac
     2581 cataatattt gtaattatcg tttttgtggt tatagcatct attcatcatg cctttccaaa
     2641 ttctgtaaag tcttgtagat gaataacctt tagcgttgtc atttttacgt gcacatccac
     2701 atgattttgt ataaccactt cttaatttac ttccaataac aactgtttta tttccgcagt
     2761 cgcaaagaca attccatcgt gcattgccac ccttagaatt gttacggttt ctattaatga
     2821 ctgtcaatga tccaaatcta agtccatcta attcttttaa gtgacaatat tctcccattt
     2881 ttgtctccta ttcaggttgg tattctcgag cattgatgcc ttttggtagg cgccatccgc
     2941 tagcagcaat gcggttaatc atgtttgatg ctacttcaaa tttccacatt ccaacgtttt
     3001 taaatccgta tcgttctagc attctgattt gtttaggtgt tgttaggcca gtttctctcc
     3061 tcttggccaa tcgctccaat aatttagtag ctttacctgc atttccaatg tcgtcggtaa
     3121 aaataccaaa tttttctaaa gcgttctttt gtttatcaga aacaggagcc atttcccagc
     3181 cgaattctgg tacatagtcc actaagtctt cagcattgat agacatttca aattgcaacg
     3241 gatcaactaa tgcacgttta cgcttacgca tttctgctaa ctgcttagcg agtgcttctt
     3301 ctcgttctgc gacaacatct ttgctagcta cttcttcagc ttctaacaat tcaaaggtta
     3361 cttcagtttc ctctgtcatg ttatcgacca ttttttgagc aacttctggt gtctttgcaa
     3421 ttagatgagc aggacgacaa agttcatggc gttcggtatg ccataaaaaa tctaataata
     3481 gtagattttc tttccccggc gctaatcgtg tgccacgacc aaccatttga ctataaagag
     3541 ctcttacttt tgtgggtctc agtacaacta cgcaatcgac tgtcgggcaa tcccacccct
     3601 ccgttaaaag cattgaattg catagtacat tatatttatc attatcaaag tcttctagga
     3661 cttcagcgcg gtctttcgat tccccattga cctctgctgc tttaaatcct ttttggttta
     3721 gaatatcacg aaatttttgg gaagttttaa caagcggaag aaatacaact gttttacggt
     3781 ttgcgcattg cttcaccatt tcatcggcaa tttgttctag gtacgggtca agcgccgtcc
     3841 caatctcact tgctttaaaa tctccagctt gctgactaac tgtagataag tccaaagtca
     3901 acgggatagt aactgcggta attttagata agtaaccaga cttgatggca tctactaagg
     3961 aatattcata agctaaacta tcgaaatact tacctagatt ttgtttgtca ccgcgatctg
     4021 gtgtcgctgt cactcccaaa acattgctac tatcaaagtg ttgcaaaact ctttggtaac
     4081 catcagagat ggcatgatgg gcttcatcaa taataattgt gtcaaaatga tttgtaggaa
     4141 acttactcaa acgcttctct ctctgcatcg tttgaactga tccaactaca accctgaacc
     4201 atgaccctaa agaagtattc tctgcttttt ctaatgctgt ccctaatcca gttgctgtca
     4261 tcaatttatc actagcctgt tctaaaagtt cggacctatg agccagaacc aaaactctct
     4321 ctccgagcct tacacggtct tcgataattt tcgaaaagac aatagtttta ccgcaaccag
     4381 ttggaaggac aagaagagtt ctcttcttgc cgtcttccca ctcgtgttgc actgctgacc
     4441 ttgcctcttc ttggtaaggt ctaagctgca ttagaatcct ccccatccgc cttgaggttg
     4501 ttgaggtgtt tgcatttgac cttgttgata tgactgcgtt gctggttgtt gtgcaaaatt
     4561 agtgccttgt gcctgttgtc cctgatatgt aggttgagca gattgttgat gtgtcatttg
     4621 ctgtggttgt gcgacatctt tatttaatac ttttgtccaa tcaacttcat cagcgtaaat
     4681 catagatttg atgttatcgt attcgcgatc agcataatcg ccggtgcctt tacgtttatt
     4741 aatacgagca acccccttag ctccgatgac ggtattccag ttcattttaa gtggttcccc
     4801 atgtttcttt tgaccaatag ctccaaagaa agctgatagc ataccttctg ttgtagtatg
     4861 taggaataag ttatgagtta attgcgcgat tccttctgat gtttctattt gaatcgtaac
     4921 gaccgccttg ttacacgctg gtaattttcc tggactcttc ggattaggtg tgtgacgagc
     4981 acgctccata ttggttactg tgaattgata atcaccaggc actaattgaa caaaacctac
     5041 actgtcttgt gtaatttcat catcccatcc caattcacgg tcaaagttgt tattgtattc
     5101 agtcattttt atttctccta ttttctatta agctaaaatt gtaatgttag tttgatcagc
     5161 aagttcttct tttaagtagt tagcaatatt attgatagca tctaagcgcc actttccacc
     5221 atctgcttca aacaaagcca tattagcaag ttgattaatg cggaaaatga actcacttga
     5281 aggttgttct acttcagaaa atgtgcggta tggacgtaat gtaactgggt ttggcgcctt
     5341 agcttttgca aggcttgcca caccttgttt aattgtggca gtttgaccaa tgccatcatc
     5401 aacaatttct gagccatttt cgatttttaa tgcactagcg aattcaataa ctgtgttacg
     5461 atcgtcagcg ttaacgaata atgattgcag actaatatta aaatcagaag gtgaatcata
     5521 gcggccaaat tgaacatttg gaataattgc ctccacagtt actaacacat ttcgattaag
     5581 gtcgtcatca tcttcttcgc aaacaataac ttcacgaggg ccttctacaa tgaccataag
     5641 acgcttggaa ttaatattgt tcatatctga tttaagataa tcaattaaac tgtcaagcgt
     5701 gtttaaattc aatgtttttg gataatactt agttttaagt tcaaccaaag aatatttgtt
     5761 gcgatcataa tactctttac catcactacc caagatagtc ttaggttttt cgtgagctgt
     5821 atttactgca tattccaaag cttctttaat gttttctgtc atgttagttt cctactttct
     5881 tttgcttatt aaaatcaata acatcttcat taataccttt ttcaaggtga tcaattggtt
     5941 caccgatgtc agttcttagc actgcctcat cgtcaaaata agtttgacca ggcatcgcgc
     6001 ttttgagctc gttagcataa accttaccat ctttttgacc aacaaggacc gttgtagcca
     6061 cgcctgtctg tggtgctagc gttgacttaa cttccatagc tgttgaaaca gtttgacggg
     6121 catcgtctgg tttcatagtt aacgtaatag ttactttacg ggctactttt gtttctgtat
     6181 taggatctaa gatattgtca aagacttttt ccaattcctt gtctactttt tcttgtaagc
     6241 caccttctcc gatggcagac aagtctaatt tgattgtttt attcattgtt tgttatacct
     6301 cgctataatt tctaattccc aaaacctatg cgccttccac agtaaagggt ggctcgacag
     6361 tacgcacttg cgttttaatc acttctaacg tagctaacca attggccaca atcatattcc
     6421 aataatctga cgggaagttt tcaattggtg tcattggcgg gaagtgtcct cggatgtttg
     6481 ctactgccac aagctcatca ggagtgacct gctctgccga catcaagtca gtcaatgctt
     6541 ttggcatact tggtgggtag gctaccggcg cagatgtcgg ttgcataggt ggttgttctg
     6601 gtgctttttc agcttgctca tgtacggatt catgtgcggt ttgtgatgca ggagtttcct
     6661 gtactggtgg cgtttgtggc actggttgag ttggtacttg tgcgaataga tgtgcgatta
     6721 ctgcatagtc aagaggcatt tcgtctggta acccatgacg gttttttgca tcccacgctg
     6781 ggtgatgttg cgtgtatagc acacgttgac caccagttgc ctttttcttt tttgattcgc
     6841 tagtcataac tactgttttg tagttggcaa acaagaccat atcagcccat tctttgacca
     6901 agggggcagt ctgtgagctt gtctttttac caagtttcaa ctcccaacgg tcataagctc
     6961 ccatctcgtc aggttgttca aattttctca tttgtgcatg ggcagttaat acaatattga
     7021 taccgagttc aatcagttct tggagcatat tcaagaatcg acctacttcc tcttttgtgt
     7081 aggtataacc attgccccaa ccaaagtcct cgataccttt cttgccatgt tgagcacaga
     7141 catcatctac tattaagctt tctgcccaat cgattgtgtc aatgaccagt gtcttgcaac
     7201 atgttggatt cgctttaatc catgcaattt gattttttag catagtgtag cttgttggtt
     7261 tatccatacg ggccacatcc atattgtctg ttgatccctc cgtatcaatg aacaatggtt
     7321 caggaaaatt agcagcaaaa ctagatttac cgattccttc tggtccataa atgatgacac
     7381 gttgagcacg tgctcgttta cctcttgtaa tttgcatatt tacctctctt tctaaaactc
     7441 acctttccaa gtcggtgcta ctgtttcagc aaaattacca ccctctgctg ttttaaagtt
     7501 atgagctttg ttttccactg agtatccatc ttctataatg atggcacact cttcaccaat
     7561 tgatacacgg gttgcaatag cttgcaaacc ctcctgctct agccgtttac caaattgttc
     7621 aagtgtgata ttgtccatct gttcgagttt atcaatcaaa acaaagccac aatctggttt
     7681 tagtttacgt acaatagcag ttgctactat tagttgttgg ctgcctgaca tgttatccca
     7741 ctcttgacca aggtaaagta actttccatc atctactgat aagccttcaa gcggcaaatc
     7801 tgcattaatc aataagtctc ttttttgttg acgaattgac tcaatatcat tagtcaatat
     7861 gttatattgt tcacgctgct gtttagcatc ttcttcagct ttatctttat caaagttagc
     7921 gcgcactttg cggttgactt catcaatatc ggcaatattc gcctcgattt cagctgttga
     7981 ttcatcatgt aaatccatag cgtcagtatt tgctattttt aaatcctcgc ttaacttatt
     8041 cgtcttagct tctgcatctg ctaataactt gcgtaaacgg tcaacttcca agatagattg
     8101 attataatca tagcgaatac gttctacatt ttgacgcttt tgagcattct ctccgttttt
     8161 ggctaagata gcttggtgtt gttggataag ctcagaaata cttaccagtt gtttaggtgc
     8221 gtcaggataa tatgtcatct ccttagcaaa cttttctttt tggtctgcta taacaccgat
     8281 agcgtgacgt tgattgtata tttctttttc ttttagctct aactctgcta gctgatcacc
     8341 gacaccaata atttcaagca agacatctgc tttctctttt ggtgtactat ccataaattt
     8401 cggcaagtta atagccaatt cttctacaaa gctatctagc aactgctgac cgcctttttg
     8461 gccgtttgga tcaattactt taagactggc atttttgcct ttccgttcaa caataagtcc
     8521 atttgacatg gtaattttaa gcgtaggtgg cacttgcgat ccttcgcgca tagcttgact
     8581 aggcttgtat ttattaccgc ctagtgccca agcaatagca tctaatacgc ttgttttgcc
     8641 ttggttatta ttaccaccga taatggtcaa gcctgttgca gatggttcaa tctttaccgc
     8701 tttaatacgc ttaacgtttt cgatttcaag tttgtttatt gttattgaca ttataattct
     8761 cccttgttcc atgcttttaa cagggctatt tgatcaattt catccaattc agcaatgcat
     8821 ttatctagcg tacttttcaa gataaaatca ctagcaataa tgacatctaa taaatttgct
     8881 ttagctgtaa ctacaaagta atgttccgcc aattcattat ttaacctgcg attttcgtct
     8941 cgcaaaaatt cgttgtcgtt aattagttgt tgtttcatac tttttttctc atataagcgt
     9001 ggaatgcatc atactgcttt tcagatgaca atcgcttagt atcgtgttta atcggttcct
     9061 gtttttttgg ttttgcgaaa ataaaatcta atagtttcat gttatactcc tagttctaat
     9121 aaaatttgat agtaacctgc aaaagaaatt ggcactgtta tattttctgg ctcacattgt
     9181 gaaccatctt ttcgaaaaca gtgaactttt ggggtccatt gctctttttt gtttttcatg
     9241 gtaaaattgc ctcataagaa tctgtctgtc ccgtaaactg ctattttaca gggctttttt
     9301 atttgcatca tcgttccatt acgggaacgt ctttatcaaa aaaaattgct attttttcga
     9361 ttggtaatcc aaaaatcaaa gtaatttttg caagctcatc agctccaatt gaaacaatac
     9421 cgttctctct ttttgcatat ggtgttcgtg ttttccaccc catagattgt gcaacttcac
     9481 attgtgtcat accgctagca attcgctcgg cttttagtcg cttcaaattg attgtcatat
     9541 acatactcct ttttttattt ttgttcccgt tttggaacac ctttattata aaacctacgt
     9601 tccaaattgt caacgatttt ttctaaaaaa aatacgttta tttgtttttt agcgttttat
     9661 tgtatttttt tgggaacgat ggtataatga tattactaaa taacattaag gataacaaaa
     9721 catgagaaca aacagcgaaa ttatttcttt gatacagagc aaagtagaag aaagaaagat
     9781 gtcaatgagt gagttggcta gaaatgttgg tattgcaaaa tcaacgatgt ctagatattt
     9841 taataaaaca agagaatttc ctttaaacag ggcagatgat tttgctaaag cacttaatat
     9901 atcaacagag tttttacttg gtattgattt aaatagcgaa gtagatggtt cggaactgct
     9961 tggaatatac cgcgaattag aagaacaacg aagagtaatt gttttagata cagcaaaaga
    10021 gcaattggaa gaacaaacaa aaatcatcaa tttcagtgat aaaaaaactg atatcgaaga
    10081 tatttttgaa gttaaaggaa cgacttttgc tgcagcagca agcggatttg gtagaggttt
    10141 tgaagcagat gactatgata cttatacagt ttatacagac gaagaacctc cacattatga
    10201 ttatgctatt ggcataagag gcgatagcat gttaccaaag tacgagcaag gagatatgct
    10261 ttatatcgtt gataaaggaa tgtcaacata cagcggacaa ttatgtattg ttgtacataa
    10321 cgggaacact tattttaaaa aagtttacac tgaacatgat ggacttcgat tagtatcact
    10381 taataaaaaa tatagcgata tttttattgg ctatccacca gcagaagata catacattaa
    10441 aatatatgac gttgttggta gttttacacc gattgaattt taactaatgt ttttagagag
    10501 gacaatgata tgacgttaaa acaactttta aaatcaaaaa atctgggaga atattttgaa
    10561 gcaaaaaaca atgcagaaaa gatgaatgag attgaaaacc gtggcttaaa gcaagtaatg
    10621 aaaaactcag tacgacagac ccaattaaat agcgaaataa aacaagtgat aaaaaaagaa
    10681 aaagaggcgg taagatgtcc aaaatgtaaa agtactaatg ttggatttat gcagcaaggt
    10741 aaaaagacat tttctgttaa aaaggccgtc gcaggaactc ttttaatagg tggaacagtt
    10801 atgggatttt taggagaaaa agggaagaag caatggcact gtaacgagtg cagctgtata
    10861 tttgagacaa aataaaaaaa gccccacgct ctcaaagttt ggcgactctg agcgtgaggc
    10921 gaatctagta tagtaaaaac ctgctttaag taggtctctt tactgtactc attttaacaa
    10981 aaaatgaggt aaaaaacaat gagaaaagta gctatttact ctagagtatc aacaataaat
    11041 caagccgaag aaggatattc cattcaagga cagattgaag ctttaacaaa gtattgtgag
    11101 gcaatggaat ggaaaattta caaaaactac tctgatgcag gtttttcagg cggtaaactt
    11161 gaacgaccgg caataacaga attgattgaa gacggtaaaa acaataaatt tgatactatt
    11221 ttagtctata aactagacag gctatcaaga aatgtaaaag acacacttta cttagtcaaa
    11281 gatgtattta ctgctaacaa tatccatttt gtcagcttaa aagaaaatat cgacacttct
    11341 tcagcaatgg gaaatctctt tctcactctc ttgtcagcta ttgcagagtt tgaaagagaa
    11401 cagattaaag agcgaatgca gtttggtgtt atgaatagag caaaatctgg aaaaacaacg
    11461 gcttggaaaa caccacctta tggatataga tacaacaaag acgaaaaaac attgtcggtc
    11521 aacgagttag aggctgctaa tgtcaggcag atgtttgaca tgataatctc tggctgctca
    11581 atcatgtcaa ttacaaatta cgcgagagac aatttcgtcg ggaatacgtg gacacatgta
    11641 aaagtaaaaa gaatattaga aaacgaaaca tataaaggat tagtgaagta tagggaacaa
    11701 actttctcag gtgatcatca agccattatc gacgaaaaaa catataataa agcgcaaata
    11761 gctttagcac ataggacaga tacgaaaaca aatactagac cttttcaagg gaaatatatg
    11821 ctttctcaca tagcaaaatg cggttattgt ggtgcccctt taaaagtatg cacaggaaga
    11881 gctaagaacg atggtacgag aaggcaaact tacgtttgtg ttaataaaac agaaagtttg
    11941 gctagacgaa gtgttaataa ttataataac caaaagattt gtaacactgg tcgatatgaa
    12001 aaaaaacaca tcgaaaaata tgttattgat gttctttata aacttcaaca cgataaagaa
    12061 tatctt
//
LOCUS       DAUDYX010000055        11279 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.056, whole genome shotgun sequence.
ACCESSION   DAUDYX010000055 DAUDYX010000000
VERSION     DAUDYX010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11279
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.056"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(112..1254)
                     /locus_tag="VHD02_001595"
     CDS             complement(112..1254)
                     /locus_tag="VHD02_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEQ5498190.1"
                     /translation="MTINRVLKDDNLLQYTEKEFIEDMKILVAIDSFKGSVTSPELNT
                     SVAQALLSVDKQLVIETRAIADGGEGSLAALSQTVAGRWHQVKTIDLLRRPIKVAYYR
                     HAKQAFIESASIIGIDKITPNSVTYAQATSYGLGLAVKDAIQKGATQIEIMLGGTGTS
                     DGGKGFLESLNYDFMTGRSYLDTLASPVTLLGLTDVTNPYHGPQGFAAVFGPQKGGSL
                     SQIEETDQIASNFAKKVFCQTTIDLQTIPGSGAAGGLGGAIVLLGGTLTSGFSRIAEL
                     LNLDNSLQSCDLVITGEGCLDTQSQSGKVPVAIARMAKKYQVPTIALCGSVKIETGLA
                     AEDFLAVFSIQQQPISLEAAIDKTTTLSNIKILAANLMLLIAQFNK"
     gene            1535..4513
                     /locus_tag="VHD02_001596"
     CDS             1535..4513
                     /locus_tag="VHD02_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEQ5498191.1"
                     /translation="MGNQTKTELELEKELIHLLETGESQWTYRKELKTEDALWDNFFK
                     ILAQNNTQYLNEEPLTASEKEQIKNQLNFVNYYEAAKWLAGENGIAKVQVQREDAKLG
                     TIRLEVVKADNVAGGTSVYEIANQVAFSGSRDRRGDVTLLINGLPMIQIELKSQNHQC
                     IEAFNQVKKYDKEGQFRGIFSTLQMFVVSNKTDTRYIAAAKENKLNPNFLTQWVDQNN
                     KPQKDLFAFAKEVLSIPRAHQMVMTYSVIDDDKKALILLRPYQIHAIEAVAEASRHRK
                     SGYIWHTTGSGKTLTSYKVARNILQIPAVEKSIFVIDRKDLDNQTASAFQSYAQNDIF
                     DVNETEDTRQLIKNLESSDRRVVVTTIQKLNAMISQMESYDTPKFKKLKERLAHLNVV
                     FVVDECHRAVTPERQRYLTNTFRNSRWYGFTGTPIFVENKRAQLGDLAQTTEQQYGKC
                     LHQYTVKEAIHDKAVLGFQVEYKTTIPDMPEDSIPEEAYDHEEHMLAVLNSIINQSRK
                     KLGFNNGIGQTFEGLLTVKSIARAQAYYDLIQKVKAGETDLVISKKVKEKLPDFPKVA
                     ITYSITENDNASISRQDKMTKNLEDYNHLFGTNFTIDNLQGYNRDLNDRLARKKDKFK
                     DRHEQLDLVIVVDRLLTGFDAPCLSTIFIDRQPMKPQHIIQAFSRTNRIFESRKHYGQ
                     VVTFQTPLRFKEAVDKALSLYSNGGENDVLAPSWEEEKARFFEKVTVLKNIVPDPDAF
                     PTIESAQTAFLKQYAKAFQAFDKLFASVQVYSDFNETLLSEVGLSDEVIDTYKGTYQN
                     VIAEIRKRREDDEAIPEINIDYELESVQMDDINYHYILTLIQAFVDQEQEALQERLND
                     NPMDQYIQDLAKSNPAMADSLAELWQDIQKEPKAYEGKSIVYELDNLIGDKIQRAIKH
                     FADQWKADPDKLAFVATNYHSANSTKQVGMSTLKESLDYQAYKEKQSDSAMNKLKYKS
                     QFERELVQFIRDQIQPLKGH"
     gene            4526..5721
                     /locus_tag="VHD02_001597"
                     /pseudo
     CDS             4526..5721
                     /locus_tag="VHD02_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527759.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            5734..6525
                     /locus_tag="VHD02_001598"
     CDS             5734..6525
                     /locus_tag="VHD02_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEQ5498192.1"
                     /translation="MAEKTTSLRQALWHSADQLRGQMDANDYKNYLLGLIFYKHLSDK
                     LLIAVCDNLEEHFNTFTDAQKIFEDAYQDQGLKDDLISVVTGDLGYFIEPTLTFEKLI
                     QDVYHNTFQLESLAQGFRDIEQSGEDFENLFEDIDLYSKKLGSTPQKQNQTISNVMKT
                     LNEIDFEAVDGDTLGDAYEYLIGEFASESGKKAGEFYTPQAVSHLMTQIVFLGREDQK
                     GMTLYDPAMGFRVIIMTQANSQVNTRVLELLPKFKIKKMNRWCAV"
     gene            6585..7433
                     /locus_tag="VHD02_001599"
     CDS             6585..7433
                     /locus_tag="VHD02_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HEQ5498193.1"
                     /translation="MSKVKKETIEAKGFAIQIYTEDFQNDYISLTDIARYKSDEPSDV
                     IKNWMRRKDTIEFLGLWESLNNMNFNSVEFDRIKSEAGYNSFTLSPKKWVEKTDAIGI
                     ISKGGRYGGAFAHTDIAFEFASWISAEFKMYVIQDYKRLKSDENSKLSLGWNLNREIS
                     KINYKIHTDAIKEYLLKDLTNEQLSYKYASEADMLNVALFNKRAKQWREENPELKGNM
                     RDYASLNELLVLANMESYNAILIGKGMDQKERMIELRKLARTQLMSIEKLNNTGIKSL
                     EDKSKK"
     gene            7444..7725
                     /locus_tag="VHD02_001600"
     CDS             7444..7725
                     /locus_tag="VHD02_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498194.1"
                     /translation="MRWALKIILFPIILILLILIAFLKFIIKVSGMILGIISFLVFIG
                     AVACFIQKDMATGIAALLLAFLISPYGLPKIALWITAYLEVAKDTLKEI"
     gene            7801..8838
                     /locus_tag="VHD02_001601"
     CDS             7801..8838
                     /locus_tag="VHD02_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEQ5498195.1"
                     /translation="MSMYLFDEQPILANKALARELGLNEALVLQQINYWIEINKKSGN
                     NYHDGKYWTYNSIRAWQEKDFDYLSVDTVKRTFSKLEKAGYLLVGNYNKDPRDKTKWY
                     TINDKKLEELYLELNQRKQEKERKILEQVSKSAMPNALGQNAPMEEGKINQCNEAFST
                     DAFDENLPVHYGNLHEPLPEITTNNSSKITSDISTEKSSHLPIYERDLFYSGDGMEEG
                     RKQIKSYKSCLEELRESTGYNEHMKLGNKIIAKTFDEIIKVLADVMVLNAEDTVTINQ
                     TKLPAYVVQERFRELDSSHMEYLVNALSENESKIRNVRAFILTAAYNAPSNMDAYYTA
                     LVSYDMREGGY"
     gene            8838..9323
                     /locus_tag="VHD02_001602"
     CDS             8838..9323
                     /locus_tag="VHD02_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEQ5498196.1"
                     /translation="MINEEISNRVVNIEVNVLKATYQEILSQVKKLQQRSKQHGGLDK
                     LIKAEGNEVKLKDMVKKGQLEEINVKDGELKELKKELNKHGVKFSVMKDKETGTHSVF
                     FQAKDTKVMNKAFQNVLSNIEKKEKNKESIHKNIKKFKELAKNTISKDKVKNKQKEQS
                     L"
     gene            9320..11101
                     /locus_tag="VHD02_001603"
     CDS             9320..11101
                     /locus_tag="VHD02_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEQ5498197.1"
                     /translation="MIDKILKDIKGLFKVQDKAKFLKQNIPYLAFFYVGNIFSHHVRA
                     YTGGDVIDKIFQGILELNTMSLLPSIHVADILMGVGVAALIKFIVYTKGKNAKKFRQG
                     KEYGSARWGTRKDIEPYMDEKFQNNILLTQTERLTMNGRPANPKYARNKNVLVIGGSG
                     SGKTRFYVKPNLMQMHSSYCVTDPKGTIVIECGKMLEDNGYEIKILNTINFKKSMKYN
                     PFAYLRSEKDILKLVQTIIANTKGEGEKAGEDFWVKAEKLYYTALIGYIFYEAPREEK
                     NFATLLDMIDASEVREDDETYMNPIDRLFEALEKKEPTHFAVKQYKKYKLAAGKTAKS
                     ILISCGARLAPFDIQELRDLMKEDELELDTLGDRKTALFVIISDTDDTFNFVVSIMYS
                     QLFNLLCDKADDEYGGRLPVHVRCLLDEFANIGLIPKFEKLIATIRSREISASIILQA
                     QSQLKAIYKDNADTIVGNCDSTLFLGGKEKTTLKELSETLGKETIDLYNTSETRSNAN
                     SYGLNYQKTGKELMSQDEITVMDGSKCIFQLRGVRPFLSDKFDITKHKNYKLLEDYDK
                     KNVFDIEDYIKRKGKVKFNRNTVITRL"
BASE COUNT         4043 a         1888 c         2128 g         3220 t
ORIGIN      
        1 caaaatgttt ctgtttttca tactatgcta atgagttagc tcgtgcgtct ccttcttcat
       61 aggttacttt taaccgccag atgaatgcta cacaatcgaa aatagatgag tttacttatt
      121 aaactgagct atcaatagca taaggttagc tgctaatatt ttgatattag aaagtgttgt
      181 tgttttatct atcgctgctt ctaaggaaat tggctgctgt tgtattgaaa aaacagccaa
      241 aaagtcttct gctgctagtc ctgtctcaat ctttacagaa ccacatagag caatcgttgg
      301 aacttgatat tttttagcca ttcttgcaat cgcaacaggt accttgccag attgtgattg
      361 tgtatccagg catccttctc cagtaataac taaatcacaa gactgaaggg aattatccag
      421 atttagtagc tctgcaatcc tgctaaaacc actagttaag gtgcctccta ataggacaat
      481 ggcacctcct aatcctccag ctgctccact tcctggtatt gtctgtaaat caatggttgt
      541 ttggcaaaaa acttttttag caaagttact tgcaatttga tcagtttctt caatttgact
      601 aagactccca cctttttgag ggccaaagac cgcagcaaat ccttgagggc catgatatgg
      661 attggtcaca tcagtcagcc ctaatagcgt aacaggtgat gctaaagtat caagataaga
      721 tcttcctgtc atgaaatcat agtttaaact ttccaaaaac cctttgccac catcagaagt
      781 gcctgttcct cctagcatga tttcgatttg agtagctcct ttttgaatgg catctttaac
      841 tgccaaaccc aaaccatagg aagtggcttg tgcataggtt actgaattgg gggtgatctt
      901 atcaatacca ataatactcg cagattcaat aaaagcttgc tttgcgtgac gataataagc
      961 gactttaatc gggcgtctta atagatcaat ggtttttact tgatgccacc tccccgccac
     1021 ggtttgtgag agggctgcta aacttccttc tcctccatca gcgatagctc ttgtttcaat
     1081 aactaactgc ttatcgacag aaagaagggc ttgagctaca ctagtgttaa gttctggaga
     1141 cgttaccgat cccttaaaag aatctatggc taccaaaatt ttcatatctt caataaattc
     1201 cttttctgta tattgtagca gattatcgtc ctttaaaacc ctattaatag tcaagctaga
     1261 tctgtcttaa gttttttatc tattttgaga actcgacctt tttactaatg ttgtcttatt
     1321 actttcttca ccttaaatta ttgtcaagca aactcaaaaa tacaagaact ttctcgattg
     1381 catttacata gtcatagctt tgtttagttg ctaggatctt aagcattcaa ctaatcaaaa
     1441 aaagactgtt tggaaggttt caatagcttt gccattctaa aaaggatccg ccaatgatat
     1501 aatattacta tatcatgtcg aatggggatt aaaaatggga aatcaaacta aaacggaact
     1561 tgaacttgaa aaagagttga tccatctctt agaaacaggt gaaagtcaat ggacatatcg
     1621 taaagaatta aaaactgaag atgctttatg ggataatttc tttaagatct tagcgcaaaa
     1681 taacacgcaa tatctgaacg aggagccttt aacagctagt gaaaaagagc agattaagaa
     1741 tcaacttaac tttgtcaatt actatgaagc ggctaaatgg ttagctggtg aaaacggcat
     1801 tgctaaagta caagtgcaac gcgaagatgc aaaactagga acgattcgtt tagaagtcgt
     1861 taaagcagac aatgtggctg gtggaacttc tgtttatgag attgccaatc aagttgcttt
     1921 ctctggttct cgtgatagac gaggtgatgt gacacttctc atcaatggct tacctatgat
     1981 ccaaattgaa ttaaaatctc agaatcatca atgcattgaa gcttttaacc aagtcaaaaa
     2041 gtatgataaa gaaggccagt ttagaggtat cttttcaacc ttacaaatgt ttgtggtctc
     2101 aaataaaaca gatacaaggt atatcgctgc agctaaagaa aataaattaa accctaactt
     2161 cttgacccaa tgggtagatc aaaacaacaa accacaaaaa gatctctttg cctttgccaa
     2221 agaagtccta tctatccctc gtgctcacca aatggtcatg acttactctg tcattgatga
     2281 tgataaaaaa gcactaattc tccttcgtcc ttatcagatt catgcaatcg aagccgtcgc
     2341 tgaagcgagt cgtcaccgca agtctggcta tatctggcac acaacaggtt ctggaaaaac
     2401 cttaacttcc tataaagtgg ctcgcaatat ccttcaaata ccggccgttg aaaaatccat
     2461 ctttgtcatt gaccgaaagg atttagataa tcagaccgct tctgcttttc aatcctatgc
     2521 gcaaaatgat atctttgatg tcaatgagac agaagatacg agacaactca tcaaaaattt
     2581 agaatccagt gatcgtcgtg ttgtggtcac caccattcaa aaactcaatg ccatgatcag
     2641 tcagatggaa agttatgaca ctccaaaatt caagaaacta aaagagcgtc ttgcacactt
     2701 aaatgttgtc tttgttgtgg acgaatgcca ccgtgctgtc acaccggaac gtcaacgtta
     2761 tctcacgaat acttttcgta acagtcgctg gtatggcttt accggcactc ctatctttgt
     2821 ggaaaataag cgtgctcaat taggtgattt agcccaaaca acggaacagc aatacggcaa
     2881 atgtctccat caatacaccg ttaaagaagc cattcatgat aaagcggttc taggctttca
     2941 agttgagtat aagacaacca ttcctgatat gcctgaagac agtattccgg aagaagctta
     3001 tgatcatgaa gaacatatgt tagctgtctt aaatagtatc attaatcaat cacgtaagaa
     3061 gcttggcttt aataatggga ttggtcagac attcgaaggc ttattaaccg ttaagagtat
     3121 tgctcgtgcc caagcctatt atgatctgat tcagaaagtt aaagctggtg aaacggattt
     3181 agtcatttct aagaaggtca aagaaaaact accagacttt ccaaaggtag ctattactta
     3241 ttctatcact gaaaatgaca atgcgtctat cagccgacaa gataaaatga ccaaaaatct
     3301 agaagactat aaccacctct ttggcactaa ctttaccatc gataacttac aaggctataa
     3361 cagagattta aatgatcgct tagcccgtaa aaaagataaa ttcaaagacc gtcatgaaca
     3421 attagacctc gtgattgtgg ttgaccgtct cttaacaggt tttgatgcgc cttgtttatc
     3481 aacgatcttt attgatcgcc agcctatgaa acctcagcac attattcaag ctttctcacg
     3541 aaccaaccgt atctttgaaa gtagaaaaca ttacggtcag gttgtgactt tccaaacacc
     3601 acttcgtttt aaagaagctg ttgacaaagc gcttagcctc tactctaatg ggggtgaaaa
     3661 cgacgttttg gccccttctt gggaagaaga aaaagcaaga ttctttgaaa aagtaacagt
     3721 attaaaaaat atagttcctg atccagacgc tttcccaact attgagagcg cacagacagc
     3781 ttttctcaaa caatacgcca aagcctttca agcgtttgat aaactctttg catctgtaca
     3841 ggtctatagt gactttaatg aaacactgtt gagtgaggtt gggttatcag atgaagtgat
     3901 tgatacttat aaaggcactt accaaaatgt cattgcagaa atacgaaagc gaagagaaga
     3961 tgacgaagcc atcccagaga tcaatatcga ctatgagcta gaaagtgttc agatggatga
     4021 tatcaactat cactatatct tgaccttaat tcaagccttt gtcgatcaag aacaagaagc
     4081 tcttcaagaa aggctcaatg acaatcctat ggatcaatac atccaagatt tggcaaaatc
     4141 aaaccctgct atggctgact cactagctga gttatggcaa gatatccaaa aagaacccaa
     4201 agcctatgaa gggaaaagca ttgtttatga gttagataac ctcattggtg ataagatcca
     4261 aagggccatc aaacactttg cggaccaatg gaaggcagat cctgacaaat tagcctttgt
     4321 ggcgactaat tatcatagtg ctaactctac caaacaagtg ggcatgtcaa ccttaaaaga
     4381 atccctagac taccaagcct ataaagaaaa acaaagtgat tctgccatga ataaactcaa
     4441 atacaagagc caatttgaac gggagttagt tcaattcatt agagatcaaa tccaaccctt
     4501 aaaagggcac taggaaagga aagagatgac aaaatctaaa cagcctcaat atcgctttga
     4561 cggttttaaa ggggagtggg aagaaaaaga actaggggat attgttcaaa ttacaatggg
     4621 ccaatctcca tcaagtcaaa attatactac aaacccttct gattatattc tagtacaagg
     4681 aaatgccgat ataaaaaatg gatatgtttt tccgcgtgtc tggacaacac aaattacaaa
     4741 acaagctgac aaaggtgata ttattcttag tgttagggct ccagttggtg atgttggaaa
     4801 gacaaattat catgttatta ttgggcgtgg agttgctgct ataaaaggta atgaatttat
     4861 ctttcaaata ttaaaatact tgaaagaaat tggttattgg aaaagaatta gtacaggttc
     4921 aacttttgat agcattagct ctagtgatat caaatatgct aaaattcaaa ttccctccct
     4981 gaacaagaag ccattggcga acttttccaa acggttgatc aattgattca gttgcaggat
     5041 caaaagctag caacccttaa agaacaaaag caaaccttcc tcagaaagat gttcccacct
     5101 caaatacaaa aagtccctga aatccgttta caaggtttta aaggagagtg ggaagagaag
     5161 aaattggggg aagtatctac tcatagaagt ggaacggcaa ttgaaaaata ttttgattcg
     5221 gaaggtgaat ttaaagtcat ttcaataggt agttatggaa ctaataacct ctatgtagac
     5281 caaaatatta gagcagtctc aaatgagctt actaattcta aattagttgc ctccggtgaa
     5341 ttaacaatgg tactcaatga taagacagcg aatggggcaa taatcggaag atgtctactt
     5401 attacagaaa ataataaata tgttgttaat caaagaacag aaattattag accagatatt
     5461 aatatttcct cttactatct tttccattat ttaaatggtg aatttcgtaa tggtatcatt
     5521 aaaattgctc aaggtggcac tcaaatctat gtaaattatt catctgttga acaattaaag
     5581 ataaatatac ctacattaaa agaacaagaa gccattggta atttcttcca aactttagac
     5641 caacaaattg ctcaatctga agaaaaacta acagaactca aagcactcaa acaaacatta
     5701 ctcaatcgtt tattcgtata agaaaggact cacatggcag agaaaacaac ttcactccgt
     5761 caagcactat ggcattcggc cgaccaatta cgtggtcaaa tggatgccaa tgactataaa
     5821 aactatcttc ttggactcat cttttacaag cacttatctg ataaattact catagctgtt
     5881 tgtgataatt tagaagaaca ctttaatact tttacagatg ctcaaaaaat ctttgaagat
     5941 gcttatcaag atcaaggcct aaaagatgac ctcatttctg ttgttactgg tgatttaggt
     6001 tactttatcg aaccgacact gacctttgaa aaactcatcc aagatgttta tcacaatact
     6061 ttccaattag aatcattagc tcaaggtttt cgcgatattg aacaaagtgg tgaagacttt
     6121 gaaaacttat ttgaagatat tgacctctac tctaaaaaat taggctctac tcctcaaaaa
     6181 caaaatcaaa ccatttctaa tgtcatgaaa acattaaatg aaattgactt tgaggcagta
     6241 gatggggata ctttaggtga tgcttatgag tacttgattg gtgaatttgc cagtgagtca
     6301 ggaaaaaaag caggtgagtt ctatacccca caagctgtat ctcacttaat gactcagatt
     6361 gtctttttag gtcgtgaaga tcaaaaagga atgaccctct atgaccctgc tatggggttt
     6421 cgtgtcataa taatgacaca ggcaaatagt caagtaaata caagggtttt agagcttcta
     6481 ccaaagttta aaatcaaaaa aatgaatcgt tggtgtgctg tttagagcag ttattatgaa
     6541 aataaatgtt gtaaacagta catcagcata aaggaggaac tcatatgtct aaggtgaaaa
     6601 aagagacgat tgaagcaaaa ggatttgcta ttcaaattta tactgaagac tttcaaaacg
     6661 actatataag tctaacggat attgctagat ataaaagcga tgaaccaagt gatgtaatta
     6721 aaaattggat gagaagaaaa gatacaattg aatttcttgg tttatgggaa agcttaaaca
     6781 acatgaattt caattcggtc gaattcgacc gaattaaatc ggaagctgga tataattcct
     6841 ttacactgtc ccctaagaaa tgggtagaaa aaacagacgc aatcggaatt atctcaaaag
     6901 gaggaagata cggtggtgca tttgcacata cagatattgc ctttgaattt gcttcatgga
     6961 tttctgctga atttaagatg tatgtaattc aggattacaa aagacttaag tcagatgaaa
     7021 actctaagct atcattaggt tggaatttga atagagaaat ttccaagatt aactacaaaa
     7081 ttcatacaga cgcaatcaaa gaatatctct taaaagatct taccaacgaa cagttgtctt
     7141 acaagtatgc aagtgaagca gatatgctca atgtagcctt gtttaacaag agagctaaac
     7201 agtggcgtga agaaaatcct gagttaaagg gtaatatgag agactatgca agtctgaatg
     7261 aattacttgt tcttgcaaat atggaaagct ataatgcaat tcttattggt aaaggtatgg
     7321 atcaaaaaga aagaatgata gaacttagaa agcttgccag aactcagctg atgtcaattg
     7381 aaaagttgaa caatacaggt attaagagtt tagaggataa atcaaagaaa tagagaggtt
     7441 gttatgagat gggcattgaa aattatctta tttccaataa tattgatctt gttgatacta
     7501 attgcttttc taaaatttat tataaaagtt agtggaatga ttttaggtat aatatctttc
     7561 ctagtattta ttggagcagt agcttgcttt atacaaaaag atatggcaac aggaatagca
     7621 gcattactac tggcattctt gatcagtcct tatgggctac ctaaaattgc actttggatc
     7681 acagcatatc ttgaagtagc gaaagataca ttaaaggaaa tataagagag agttaagacg
     7741 atggaatata aatctatcgt ctttttctat gcctaaattt cagagaaagg aggcgatgat
     7801 atgagtatgt atttatttga tgaacaacct atactggcta ataaagcatt agccagagaa
     7861 ttaggcttaa atgaagctct tgtgcttcaa caaataaatt actggattga gataaataag
     7921 aagtctggta acaattatca tgatggcaag tattggacat ataactccat acgagcatgg
     7981 caagaaaaag attttgatta tttaagtgtt gatacagtaa agagaacttt ttctaagctt
     8041 gaaaaagcgg gctatctttt agttggaaac tataataaag atcctagaga taaaacgaag
     8101 tggtacacaa taaatgacaa gaaattagaa gaactttatc ttgaactaaa tcaaagaaaa
     8161 caagagaaag aaagaaaaat attagaacaa gtaagtaaaa gtgctatgcc taatgcatta
     8221 gggcaaaatg caccaatgga agagggcaaa atcaaccaat gcaatgaggc atttagcacc
     8281 gatgcatttg atgaaaatct accagtgcat tatggcaact tgcatgaacc attaccagag
     8341 attactacaa ataattcatc aaagattact tcagatattt ctacagagaa gtcctcccac
     8401 ctacccatct atgaaagaga tctgttttat tcaggggacg gaatggagga aggacgtaaa
     8461 caaataaaat catataaatc ttgtttagaa gaattaagag aaagcacagg atacaacgaa
     8521 catatgaaac tgggcaataa gattattgct aaaacctttg atgaaataat taaagtttta
     8581 gctgatgtta tggtattaaa tgcggaagat acagtcacaa taaatcaaac gaaattacca
     8641 gcatatgttg ttcaagagag atttagagaa ttagattctt cgcatatgga gtatcttgta
     8701 aatgctttat cagagaacga atcaaaaata agaaatgtaa gagcatttat cttaacggca
     8761 gcatataatg caccaagcaa tatggatgcc tattatacag cacttgttag ttatgatatg
     8821 agggaaggag gttactaatg ataaatgaag aaatttctaa tagagtagtt aatattgaag
     8881 ttaatgtctt aaaggcaaca tatcaagaaa tcttaagcca agtaaagaag ttacaacaga
     8941 gatcaaaaca acatggtggc ttagataaac ttatcaaggc tgagggaaat gaagtcaaac
     9001 tcaaagatat ggttaagaaa ggtcagcttg aagaaatcaa tgttaaagat ggtgaattaa
     9061 aagaactaaa aaaagaattg aataaacatg gtgttaaatt ctcagtgatg aaagataagg
     9121 agactgggac acattcagta ttcttccaag caaaagatac aaaagttatg aataaagctt
     9181 ttcaaaatgt gctatcaaat attgagaaaa aagaaaaaaa taaggagtca attcacaaga
     9241 acataaagaa atttaaagaa ctagctaaaa atactatttc taaggataaa gtcaagaata
     9301 aacaaaagga gcagagccta tgatagataa gatactaaaa gacatcaaag gcttattcaa
     9361 agtgcaagat aaggcaaagt ttctaaaaca gaacattccc tatcttgcat ttttctatgt
     9421 aggcaatatc ttttctcatc atgtacgagc ctatactggc ggcgatgtga tagataaaat
     9481 ctttcaagga atattggaac ttaacaccat gagcttactg ccaagtattc atgtggctga
     9541 tattttaatg ggcgtgggag tagcagcttt aattaaattt attgtctata ccaaaggtaa
     9601 aaacgcaaaa aagttcagac aggggaaaga gtatggctca gcaagatggg gaacgagaaa
     9661 agatatagag ccgtatatgg atgaaaagtt tcaaaataat atcttgctta ctcaaactga
     9721 gcgattaacc atgaatggca gaccggcaaa tccaaagtat gcaagaaata aaaatgtttt
     9781 ggttataggt ggttcaggct ccggaaagac gagattttat gtaaaaccga acctaatgca
     9841 aatgcacagt agctattgtg ttacagatcc taagggaacg atagtcattg aatgtggtaa
     9901 gatgcttgaa gataacggtt atgagataaa aatcttaaat accatcaact tcaaaaagag
     9961 tatgaagtat aatccattcg cctaccttag aagtgaaaaa gatatattga aactggtaca
    10021 gacaattatt gcaaatacca agggagaggg tgaaaaagca ggcgaagatt tttgggtgaa
    10081 agccgaaaaa ctctactata ctgctcttat tggctacatc ttctatgaag ctccaagaga
    10141 agaaaagaac tttgcaacac tccttgatat gatagatgct tcagaagtca gggaagatga
    10201 tgaaacatat atgaatccta ttgataggct atttgaagca ttggaaaaga aagagcctac
    10261 gcactttgca gtaaagcaat ataaaaagta caaattggct gcgggaaaaa cagcaaagtc
    10321 tattcttatt tcatgtggtg caaggcttgc tccatttgat attcaagaac tacgggactt
    10381 aatgaaagaa gatgaacttg aacttgatac acttggagat agaaagacag cactttttgt
    10441 tattatctct gatacagatg ataccttcaa ttttgttgta tctattatgt actcacagct
    10501 atttaaccta ctttgtgata aggcagatga cgagtatgga ggaagattac ctgttcatgt
    10561 aaggtgctta cttgatgagt ttgcaaacat cggcttaatt ccgaagttcg agaaattgat
    10621 tgcaacaatc agaagtagag agatttcagc aagtatcata ttacaagcac aatctcagct
    10681 aaaggcaatt tacaaggata atgcagatac gattgtaggc aactgcgata gcactttatt
    10741 tcttggtgga aaggaaaaga caacacttaa agaactgtct gaaacactgg gtaaagaaac
    10801 catagatctg tataacacat cggaaacaag atctaatgct aatagctatg gactgaatta
    10861 ccaaaagaca ggaaaagaat tgatgagcca agacgagata acagttatgg atggtagcaa
    10921 gtgtattttt cagcttcgag gtgtcagacc atttttgtca gataaatttg atattacaaa
    10981 gcacaagaat tacaagctcc ttgaggacta tgataaaaag aatgtgtttg atattgaaga
    11041 ctatatcaag agaaaaggaa aagttaagtt taatagaaat acagtaatta caagattgta
    11101 atatttttct gttatagctt tatgtggcat gggtatattt ggtataataa aagtcatatt
    11161 agagatagag gagatgtatg tgtgaatttt gattttaaga ttgtggattt ttttaaattg
    11221 cctactaaaa ttatgtgtgc tattagcata gctactggac ttgttttatt tttgccaga
//
LOCUS       DAUDYX010000056        10983 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.057, whole genome shotgun sequence.
ACCESSION   DAUDYX010000056 DAUDYX010000000
VERSION     DAUDYX010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10983
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.057"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            5..1723
                     /locus_tag="VHD02_001604"
     CDS             5..1723
                     /locus_tag="VHD02_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEQ5498198.1"
                     /translation="MTVFLVIFLIYFNNFFVKKRSQELGVLAILGFSKKELTQLLTLE
                     NLVILVLSYLVSLLLGPTLYFLAVLVITHLLELTLEVQWFITVNEIIESLGILVVVFL
                     INVITNGLIISKQSLIEFVNFSRKAEKKIKIRKVRAIIAITALLLSYILCLATVFSST
                     RNMLLSIGMVPVSLLIIVLVVLGTVFTIRYGLAFVVSLLKENKKRLYRPLSNIIYPKF
                     NYRIATKNKLLTVLGGLLTVTVSVAGMMVMLYAYSLNGIERLTPSAIEYNVESENGQV
                     NVTTILENDQVSLVDVDLLRLNTNPEVTITESGQTIPYFDIINYSDYKELMKAQGRIN
                     SIEGSKSLPLLINYYPTEISLGKTFNLGNAYDVTVKQVSTHNVFSFSTSVTTLVVSDK
                     LYAKLSSCFPEKEMTIRTFNGNSIRSSEAFYNQFSMVPDVISSYSKEYTVKTANIATY
                     IFITFLSILFIICTGSILYFTSLIEIMENKEEYGYLSKLGYSKKMIHRILRYETGILF
                     LIPVFIGIVNGGMLLIYYKYLFMDTLVAGNIIMLSLLLCLLFFLIIYGTFYVLTLRSV
                     TSIIKN"
     gene            1763..3325
                     /locus_tag="VHD02_001605"
     CDS             1763..3325
                     /locus_tag="VHD02_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEQ5498199.1"
                     /translation="MIRIKNITKSKFFGTAIILLQQLIALLILVYNRENLSLLFSEKV
                     ALVMTLIDTAFIWLATILRQKQGDIFKRIISIISLTIWQYLVSVLTRGTPLFLSSGLQ
                     IILLYCYTVEITNLILYGHKQFKDKLDKGLLIIIFVSITSLFVNSILFNFLFLMVFTI
                     LHLYPLLVIVLYYRSFRQQISVVRRSLILFSLLLLVILGSELYGEMLDVNQAFNNLGW
                     YLFPLIMSVIYYFKTIHDKLSFVTQRWLGDYKARIELLFLLLILCWIVLMKVLVKDFL
                     LFFIIVDASTLFCLVVISCIFYYLENSKQNFDYENRRLNYFMKSEENMRVEFSNYLHD
                     DVLQNIIAIKNLLSLENSNITHGFIVNELNDLVSGIRDEMDTYHPIVPANQTMKENIQ
                     SLFDDIVKSRKSNTLLYFNCSDNMVVPSPYGDIVYRFIKELINNAIKYGDGKDIRLSL
                     TIQSDIIIIEESNQVVEKVHSINYGRGLKSFQETLAAFDGDLELQMDTKQFTIRILLP
                     IDWKLCYEDFIN"
     gene            3306..3911
                     /locus_tag="VHD02_001606"
     CDS             3306..3911
                     /locus_tag="VHD02_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ5498200.1"
                     /translation="MKILLIDDHRLFAKSIQLLFQQYDEVDVIDTITSHFNDVTIDLS
                     KYDIILLDINLTNISKENGLEIAKELIQSTPHLKVVMLTGYVKSIYRERAKKVGAYGF
                     VDKNIDPKQLISILKKVDSGKKYFEQIESQDYVESLTDQEIAILNLSKKGFSIKEIEE
                     TLQISRRTVFNHLTHIYSKLLVNNKQEAIYKAEQLGYFMDF"
     gene            complement(4463..5362)
                     /locus_tag="VHD02_001607"
                     /pseudo
     CDS             complement(4463..5362)
                     /locus_tag="VHD02_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
     gene            complement(5446..6939)
                     /locus_tag="VHD02_001608"
     CDS             complement(5446..6939)
                     /locus_tag="VHD02_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEQ5498201.1"
                     /translation="MSKIALYIRLSVEDQMKKDESESIINQRYFLNDFLDRNDELKSF
                     QREEYIDDGYTGTNEKRPSFQRMLEEVKSGKINAIIVKDLSRFMRDYISLGDYLENIF
                     PFLGIRFIAINDGYDSAKEKGNGTDLDIQFKGLLYDFYTKDISQKVKTVTTELKKQGK
                     FLAWSPPFGYMKDPDDKHNIIIDDKTAWIVRKVYDLALTGLASRKIAAVMNEENISTP
                     NERKKELTNMDYEYNIVSSDTRDAPTWTNGTVVDILSNENYTGTYVFNMQEKSILTPG
                     SFKFNPKEEWGRVYEHHEAIISREEFEKVQAIKENNSFLKGKNTDYPWRKHSPLQGFA
                     RCPTCNHILGLTQSKFKRPDGSMRIHKYFHCRICKCNNVEHKNSRVDKLEDQVLALIK
                     EKYGEAEVKPKEKISIKDIEKKMEKLQAKKMSDFEKYKLGKMTKAKFVESKNQIDNEF
                     DRLEEKIKLSSNETEVVIDNELTREVMKKYVESVICEGSIVQKIIWR"
     gene            complement(6932..8599)
                     /locus_tag="VHD02_001609"
     CDS             complement(6932..8599)
                     /locus_tag="VHD02_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004837868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEQ5498202.1"
                     /translation="MARTSKRYIRKNEEQAQKVFYKAGIYTRLSSERKEEWREKSSSI
                     ETQVLCCKEYALKENIKVVNIYTDYEYSGTNFERPQFQEMMQDIRDRRINCIIIRDLS
                     RLGREYLEMGRLIDKVFPFLGVRFISVNDKVDTVKDLDSKKSFEVTLKNIVNDMYAKD
                     ISVKIKTSKHNRARNGYFIGSVPPYGYKVVKLKEGQKLEVDENVRFIVEEMFRLTLGG
                     KSQYEVAKHFNTMGYATGMVHYKTGRIYRQDGDPQWNKGTISKMLTNRAYTGTLVQGV
                     KQQNLAKGMKQQFVDESQYIVYENAHEPIISKEDFEKVLQGRADRLKNNAFGAEIHNF
                     ERDYENRYKGLIFNNATGKELYRRTRIYGINHDRLYYSFQNDTFTGKIDNEVRVFIME
                     RELDKAMSEKVADFITKATSKAKLIERVSKRFSESIGKLNENISKLKIKAEKEEFLIQ
                     KTYEEYSLGKIDREVYILKREITLRHIATINNEVIAIEQVVKDLERDNKTSIKWIKDV
                     FSAKKVEKLPADLIHNLVEKIIVYGNHNFEIVFKFNMDSLMGGVEDE"
     gene            complement(8599..10263)
                     /locus_tag="VHD02_001610"
     CDS             complement(8599..10263)
                     /locus_tag="VHD02_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004027196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEQ5498203.1"
                     /translation="MRNTACMYLRLSREDGDSNESNSISNQRQIIKSYAKEKNIDLSY
                     EYVDDGYSGSNFERPDFKNMIDDLNKGKFSIIMVKDLSRFGRDYIESGKYLQKIFPEK
                     GIRFISVNDNYDSDNADVSDTHLILPIRNFINDSYCRDISMKVKSSKEVKRKNGEFIG
                     SFAPFGYKKDDKNKHQLVVDTEVAHIIERIFNMKIEGYSSKAIADFLNSIGTVTPSKH
                     KENNGDNFNTGFVVKKAKWDAKMVNRIISNKVYIGVLEQGKTTKLNYKSKHEVDVSKE
                     DWIAIENAHESIVSKSIFALANKMLLRDVKQSKDKPYILSGMLYCKDCGSPMIRRKVR
                     DKIFYICSEYNNAGECSRHSVKEDYVTHATIHALNEYLSKYNELLKKVSEIDVSKFTL
                     KVDFESLNAEKRKYERLRQSLYMDLEEELITTEEFERFRKNYLIKIREIEKQIITKQN
                     IVEELKMKINDKGSFVSEIVPNLESDELNRLCLVSFIDCIEIGEDNAINFVFNNIETV
                     NLLQAIVDSDKAKSKDTHKARLISMGKLFGEHLERAEPKLAVGGVC"
     gene            complement(10362..10517)
                     /locus_tag="VHD02_001611"
     CDS             complement(10362..10517)
                     /locus_tag="VHD02_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498204.1"
                     /translation="MNKKKEVKKDKDKKQIADDYSKKITYSTSESEKLDLLDIVEMYL
                     CKSCVRI"
BASE COUNT         3369 a         1906 c         1625 g         4083 t
ORIGIN      
        1 ttttttgact gtatttttgg tgatattttt aatttatttc aataatttct ttgttaaaaa
       61 acgtagtcaa gaacttggag tcttagcaat actagggttt tcaaaaaaag aattaacaca
      121 attactgact ttagaaaatc ttgttattct agttttaagt tacttggtaa gtttactatt
      181 gggaccgacc ttatattttt tagcggtact ggtaattact catctattgg aattaacatt
      241 ggaagttcag tggtttatta cagttaatga gattatagag tctttaggaa tattagtcgt
      301 agtttttctg attaatgtca tcacaaatgg acttatcatt agtaaacagt ctttgattga
      361 atttgttaat ttctcaagaa aggctgagaa aaaaattaag ataagaaaag tcagagctat
      421 tattgctatc actgcattgc tattgtcata tattttatgt ttggcgacag tgttttcatc
      481 cacacgaaat atgctattaa gcatagggat ggtaccggtt tctctattga taattgtctt
      541 agttgtttta ggaacagtgt tcaccatcag atatggattg gcttttgtag tttcgttgtt
      601 aaaagaaaat aaaaaaaggt tataccgtcc tctgtctaat atcatctatc ccaaatttaa
      661 ctatcgtatt gcaacaaaaa ataaattatt aacagtcttg ggaggtcttt taacagtaac
      721 cgtttcagtt gccggaatga tggtaatgct ctatgcttat tctcttaatg ggatagagag
      781 gttgactcca tctgccatag aatataatgt tgaatcagaa aacggtcaag tcaatgttac
      841 aactatttta gagaacgacc aagtgagctt ggttgatgtt gacctattga gattgaacac
      901 taacccagaa gtaactatca cagaatctgg gcaaacaatt ccttattttg atataattaa
      961 ttacagtgat tataaagagt taatgaaagc tcaagggaga ataaattcta ttgaaggtag
     1021 taagtcactc ccattgttaa taaattatta tccaacagaa attagccttg gaaaaacctt
     1081 taacttagga aatgcatatg atgttactgt aaaacaagta tcaacgcata atgtttttag
     1141 tttttctaca agtgtcacga ccttggttgt ttctgataaa ttatatgcta aacttagttc
     1201 ttgttttcca gagaaagaaa tgacaattag gacttttaat ggaaattcga ttaggtcaag
     1261 tgaagcattt tacaatcagt ttagtatggt tcctgatgtt atcagtagtt atagtaagga
     1321 atacacagta aagactgcta atattgcgac ttatatcttt ataactttcc tatccatact
     1381 ctttattatt tgtacaggta gtattctgta ctttacaagc ctcatcgaaa tcatggaaaa
     1441 taaagaagaa tatggctatc taagtaagct aggttatagt aaaaaaatga ttcatcggat
     1501 tcttcgatat gaaacaggta tacttttcct tattcctgta ttcattggga ttgtaaatgg
     1561 tggtatgttg cttatttact ataaatattt attcatggat acattggtag caggcaatat
     1621 cataatgtta tctttattgc tttgtctgct tttcttcttg ataatatatg gcacatttta
     1681 tgtattgaca ttgcggtcag tgacatccat aatcaaaaat taattttaaa aatcttttaa
     1741 ctttaacgat taagtaaata atatgataag aataaaaaac ataacaaaga gtaagttctt
     1801 tgggactgca ataatcttgt tgcaacaatt aatagccttg ttaattttgg tctataatcg
     1861 agagaacctg tctcttcttt tctctgaaaa agtagctctt gtaatgaccc ttattgatac
     1921 agcttttatt tggctggcaa caatattacg ccaaaaacaa ggtgatattt ttaaaaggat
     1981 tatttctatc atctccttaa caatttggca atacttagta tctgtgctga caagaggaac
     2041 tcctctcttt ttaagcagtg gactacagat tatattgctc tattgttata ccgttgagat
     2101 tactaattta atattatatg ggcacaaaca atttaaagat aagttagata agggcttgtt
     2161 aataattatt tttgtttcta taactagcct ttttgttaat agcattttat ttaatttttt
     2221 attcctgatg gtttttacta ttttacacct gtatccgtta ctagttatag tgctatatta
     2281 taggtccttt aggcaacaaa tatcagttgt tagacgttca ttgatactat tttctttact
     2341 tttgctggta atacttggta gtgaacttta tggagaaatg ctagatgtta atcaagcctt
     2401 taataatttg ggatggtatc tgtttcccct tataatgagt gtaatatact attttaagac
     2461 catacatgat aaattgagtt ttgttaccca aagatggtta ggtgattaca aagctcgtat
     2521 agaactatta tttctacttc ttatcctttg ttggattgta ctcatgaagg tattagttaa
     2581 agatttttta ttatttttta taattgttga cgcaagcacg ttattttgtc tagtggtaat
     2641 ttcttgcatc ttttattatt tagaaaactc caaacaaaat tttgactatg aaaatagaag
     2701 actgaactat tttatgaaat cagaggaaaa catgagagtg gagttctcaa attatttgca
     2761 tgatgatgtt ttacaaaata tcatagcgat aaaaaacttg ctttctctag aaaatagtaa
     2821 tataacacat ggttttattg ttaatgagtt gaatgattta gtttctggta ttagagatga
     2881 aatggatact taccacccaa tagttcccgc taatcaaaca atgaaagaaa acatacagtc
     2941 tctttttgat gatattgtta aaagtagaaa aagtaatacc ctattgtatt ttaactgttc
     3001 agataatatg gtagtgccat ctccatatgg agatattgtt tataggttta taaaagagct
     3061 aataaacaat gctataaaat atggagatgg aaaagacatt cgtttatctt taacgattca
     3121 atctgatatt attatcattg aggagagcaa tcaagtagtg gaaaaagtac actctataaa
     3181 ttatggtaga ggattgaaat catttcaaga aacactagca gcttttgatg gagatctaga
     3241 actacaaatg gatactaaac aatttactat cagaatatta cttcccatag attggaaatt
     3301 gtgttatgaa gattttatta attgatgacc acagactatt tgcaaagagt atccaactgt
     3361 tgttccaaca gtatgatgaa gtagatgtca tagatacgat tacttctcat tttaacgatg
     3421 taacgattga tttgtctaag tacgatatta tattgcttga tattaattta acaaatattt
     3481 ctaaagaaaa tggattggag atagcaaaag aactcataca gtctactcct catttgaaag
     3541 ttgttatgtt gacaggatat gttaaatcaa tctatagaga gagggcaaaa aaagtaggag
     3601 catacggttt tgtagataaa aatatagacc ctaaacaact catttctatc ttaaaaaaag
     3661 tggattctgg aaaaaagtat tttgaacaaa tcgaatctca ggattatgtt gaatctttaa
     3721 ctgaccagga aatagctatc ttaaatttga gtaagaaagg attctctata aaagaaatag
     3781 aggaaacgtt acaaattagt aggagaacag tatttaatca tttgacccac atctactcaa
     3841 agcttttggt aaataataag caagaggcta tctacaaagc cgaacaatta gggtatttta
     3901 tggatttctg agttgcagat aatcaaacga cataagtttt acttgtgtcg ttgctaggga
     3961 tttcctgtta aatacccaga tttaaactca ttgtttttga gttctttttg attgaaattg
     4021 attgagataa cgcttttata aaaagtaggg caatgcctga tatgaaaagc tttttaaatt
     4081 tagtcaaaca aaagttgttt aaaccaggtc taaaaaaact cgtaaagctt cacaactccc
     4141 agaacgttaa tatatgctta tatatcaacg attggaacta ctttgtggtt cgcaccatgg
     4201 tttttgtgga aggatcaaaa gttgtcctga aaatttctct taaccgtgtt taagctttct
     4261 aaacgtttga gaaatcattg taacttcaaa taactatttt aaataaaaca aagaaacgga
     4321 caaagttaga tgagatgatt ctattttgat ttataggtga agaaggagaa tcttgtttga
     4381 taagtactca ataaaggttc actactcaat tttaaataaa acgccacagt agtaatcatc
     4441 tactgtggtg ttttttggtc ttttagtttc taaaagtatc caattcgtct tgtgcttctt
     4501 tggttgttcc gactagttgt ttcatgagtt gatctagttt ggcatttgtt ttggcaattt
     4561 cttgatcaat gtcactgagc tgtttagcta attctggcag tggtttgacg gggacttcct
     4621 caaaggtatc gacatagcgt gggatattta agttatagtc attttcaatg atttcatcaa
     4681 atgaggctaa atatgaaaac ttatcagaat tatcacgact cttataggca ttaaggacct
     4741 ttttgatatg gctatctgtc attgtgtttt gatttttgcc tttatcaaac tcttttgaag
     4801 catcgataaa gaagacatct ttgttggttc tgttcttctt taatatgata atagtcgtag
     4861 ggatgcttgt attataaaag atatttgatg ggagtccgat aatggtatcg atagcccctt
     4921 gttcgagtag tttttggcga atcttgcctt ctgcagcacc acggaaaagg acaccgtgtg
     4981 gtaagacaat cgccatagtt cctgtatttt ttaaatgata gaaaccatga agtaaaaagg
     5041 caaagtcagc ttttgatttt ggtgctaata caccataact tgaaaaccgt ggatctgtta
     5101 agaaaccagc agtcgctgac catttcaaag aataaggtgg gttcatcaac accccatcaa
     5161 agttggttgg ttcatctgtt ggccagtctg catccaaggt atcagcgtta cttaagtgtt
     5221 ggttttcgat ggcaacgcca tgaagcatca tgttcatacg agccaagtta taagtagaag
     5281 tattgatttc ttgaccatag taagaaacgg tatcggattg atgactatat ttcttagcat
     5341 ttaaaaggag tgatccagat ccctctcgtg tcaagcgact gacacaaaat acattgtcta
     5401 tttttagaca acaaaaagag agtgatttct cactccctct tgcatctatc tccatatgat
     5461 tttttgaact atactacctt cacagataac tgactcaaca tactttttca taacctctcg
     5521 tgtaagttca ttatctatta ctacttctgt ttcattagaa gatagtttta tcttttcttc
     5581 aagcctatca aattcattat ctatctggtt tttactttct acaaacttag ctttcgtcat
     5641 tttcccaagc ttatattttt caaaatcaga catttttttc gcctgaagtt tttccatctt
     5701 cttctctata tctttaatac ttattttttc tttgggcttc acttctgctt caccatattt
     5761 ctctttaata agtgcaagca cctgatcctc taacttatct actcttgagt ttttatgttc
     5821 tacattattg cacttgcaga tcctacaatg aaaatactta tgtattctca tacttccatc
     5881 tggtcgctta aattttgatt gagtaagtcc cagaatatgg ttacaagtag gacatcttgc
     5941 aaatccttgt aatggagaat gctttctcca tggatagtca gtatttttcc cttttaagaa
     6001 actattattt tctttgatag cttgaacctt ttcaaactct tctcttgaga taatagcttc
     6061 atgatgttca taaactctac cccattcctc tttgggatta aatttaaaac taccaggagt
     6121 caatatcgac ttttcttgca tattgaagac ataagtgccg gtgtaatttt cattggataa
     6181 aatatctacc actgtaccat ttgtccatgt tggtgcatct cttgtatctg atgagactat
     6241 gttatattca taatccatat tggtaagttc ttttttacgc tcgtttggtg ttgatatatt
     6301 ttcttcattc ataactgctg caatctttct tgatgcaagt cctgtaagtg ctaaatcata
     6361 taccttcctc acaatccaag ctgtcttatc atcaataatg atgttgtgct tatcatctgg
     6421 atctttcata taaccaaatg gtggactcca agcaagaaac tttccttgct ttttaagttc
     6481 agtagtaact gtctttactt tctgagaaat atcttttgta taaaagtcat atagcaaacc
     6541 tttaaactga atatcaaggt ctgttccatt tcctttttcc ttagcactat catagccatc
     6601 attaatcgca ataaacctaa tgccaagaaa agggaatata ttctccagat aatcaccaag
     6661 tgaaatgtaa tctctcataa accttgataa gtcttttaca atgatggcat tgattttacc
     6721 actcttaact tcctcaagca ttctttgaaa agatggtctt ttctcatttg ttcctgtgta
     6781 tccgtcatca atatattctt ctctttggaa gcttttcaat tcatcatttc tatcaagaaa
     6841 atcattcagg aaataccttt ggttgatgat actttcgctc tcatctttct tcatctgatc
     6901 ttctacagat aatctgatat agagtgcaat tttactcatc ttcaacacct cccatcaaac
     6961 tatccatatt aaatttaaag actatttcaa aattatgatt gccatagaca attatctttt
     7021 ccactaaatt atgaattaaa tcagctggta atttttcaac cttttttgcc gaaaatacat
     7081 ccttaatcca cttaatagat gttttattat ccctttcaag gtcttttaca acctgttcaa
     7141 tcgcaataac ttcattattg attgttgcaa tatgtctaag ggtaatttct cttttaagga
     7201 tatatacttc cctgtcaatc ttgcctaagc tatattcttc ataagttttt tgaattagga
     7261 attcttcctt ctccgcctta attttaagct ttgaaatatt ttcgtttagc ttacctatgc
     7321 tttcagaaaa tcttttagat actctttcta ttaacttcgc cttgcttgta gccttagtaa
     7381 taaaatctgc taccttttca gacattgctt tatcaagctc tctttccata ataaagactc
     7441 ttacttcatt atctatcttg cctgtaaaag tatcgttttg aaaagagtag taaagcctat
     7501 catggtttat cccataaatt ctggttcttc gataaagctc cttgcctgtt gcattattga
     7561 aaatcagtcc cttatatcta ttctcataat ctctttcaaa gttatgaatt tctgcaccaa
     7621 aagcattatt ctttaatcta tctgctcgtc cttgcaacac tttttcaaag tcctcttttg
     7681 aaataattgg ttcatgagca ttttcataaa caatatactg gctttcatct acaaattgtt
     7741 gcttcattcc ttttgcaaga ttttgctgct tgacaccttg tactaaagtt cctgtataag
     7801 ctctattcgt aagcattttg gaaatagtac ctttattcca ctgcggatca ccatcctgtc
     7861 tataaattct acctgttttg taatggacca ttcctgtagc ataacccata gtattaaagt
     7921 gctttgcaac ctcatattgg ctttttcctc caagtgttag tcggaacatt tcttcaacaa
     7981 tgaatcgaac attctcatca acttctaact tctgtccctc ttttaatttc actaccttat
     8041 atccataagg tggaacagaa ccaataaaat atccatttct tgccctattg tgcttacttg
     8101 tctttatctt tacagaaatg tctttagcat acatatcatt gacgatattt tttagagtta
     8161 cttcaaatga tttcttagaa tctaaatcct taacagtatc aactttgtca ttaacagaaa
     8221 taaaccttac tccaaggaac ggaaatacct tatctataag tcttcccatc tcgagatatt
     8281 ctcttccaag tcttgataag tcccttatga taatacaatt aattctccta tcccttatat
     8341 cctgcatcat ctcctgaaac tgtggtcttt caaagtttgt tccactatac tcatagtctg
     8401 tgtagatatt tacaactttg atattttcct ttaaagcata ctctttacaa caaagcacct
     8461 gtgtttcgat agacgatgat ttctcacgcc attcttcttt tctttcactg gataatcttg
     8521 tatagatacc agctttataa aatacttttt gtgcttgttc ttcgtttttt cttatatatc
     8581 ttttggaagt ccttgccatt aacaaacacc tccaactgca agcttaggtt cagctctttc
     8641 taaatgctca ccaaataact tgcccattga tattagtcta gccttatgtg tatccttaga
     8701 tttagcctta tcactatcta caatcgcttg aagtagattt actgtttcaa tattgttgaa
     8761 gacaaagtta attgcattat cctctccaat ctcaatgcag tctataaaag acaccaagca
     8821 taatctattt aattcatcac tttcaagatt aggaactatc tcagaaacga aactaccttt
     8881 atcgtttatc ttcatcttta attcttctac gatattttgc tttgtaataa tttgtttctc
     8941 gatttctcta atcttgataa gataattttt tctaaatcgc tcaaattcct cagttgtaat
     9001 aagctcttct tctaaatcca tataaagaga ttgtctaagt ctttcatatt ttcttttttc
     9061 tgcatttaat gactcaaaat caacctttaa tgtaaacttt gacacatcta tttcgctaac
     9121 ttttttaagc agttcattgt acttagatag atattcattt aatgcatgaa tagttgcatg
     9181 agtaacatag tcttctttta cactatgtct tgaacactct ccagcattgt tatattcaga
     9241 gcaaatgtag aaaatcttgt ctcttacttt tcgcctaatc ataggacttc cacaatcttt
     9301 gcaataaagc atacctgata atatatatgg cttatcttta gactgtttca catcacgaag
     9361 tagcattttg tttgcaagtg caaagatgga ttttgatact atgctttcat gagcattttc
     9421 tatggcaatc cagtcttctt tggaaacatc gacttcatgc tttgatttat aatttaactt
     9481 tgtggttttt ccttgttcca ggacaccaat ataaacctta tttgaaataa tacgatttac
     9541 catcttagca tcccattttg cttttttcac aacaaaacca gtattgaagt tatcaccatt
     9601 attttcctta tgcttggatg gtgtaactgt tccaatactg tttagaaagt cggcaatagc
     9661 ctttgaggaa taaccctcta ttttcatatt aaatattcgc tcaatgatat gagctacttc
     9721 tgtatcaact acaagctgat gtttgttctt atcatctttc ttatatccaa aaggagcaaa
     9781 tgatccaata aactcgccat tcttcctttt cacctctttt gatgatttca ctttcataga
     9841 aatatctcga caataactgt cattgataaa atttctaatt ggaagtatta aatgtgtatc
     9901 actaacatct gcattatcac tatcatagtt atcatttaca gatatgaagc gaataccttt
     9961 ttcaggaaat atcttttgca gatacttccc agactcaata taatctctgc caaaacgaga
    10021 taagtctttt accataatga tagaaaactt tcctttattc aggtcatcaa tcatattctt
    10081 gaaatctgga cgctcaaaat tagaaccact atatccatca tctacatatt cataagatag
    10141 atctatgttc ttttctttag cgtaagattt gattatttgt ctttgatttg aaatagaatt
    10201 gctttcatta ctgtccccat cttctcttga aagacgaaga tacatacacg cagtatttct
    10261 cataacaatc ctcctaaaat gatattttgg caaatagtca tttcggaaga ttacttctac
    10321 cacctatatt ttaccgcacc ctatttctct ttgtcagccc ctcaaattct gacacaggat
    10381 ttacatagat acatctcaac aatatcaagt aaatcaagct tttcactttc gctcgtagag
    10441 taggttattt tcttagaata atcatcggct atttgcttct tatccttatc tttcttaact
    10501 tcttttttct tattcatagt cttaacctca ctattttttg taataagttt tatgacattg
    10561 gtaggtgcat tggctgctac attggaatct caccaccgcc ccttattaag acgagccggc
    10621 ttaaactatt gaagtatcat tatcaccatt cgcatcatag agatagattg ccacacctat
    10681 cctttatgta ttcctattta tcgctctttt tcttttatcc ttggcactta ccagtattca
    10741 tttaaaccaa tttctcagca gaaaagtctt ggcgtaggtc ataattctcc acaaatggat
    10801 aacgtcccac cttggtctat tcagttgtca aagatcaatt agctttctgc tatgtaggat
    10861 tctttgctta ataacggaat agattttctc ttctacctca aaagcaaaaa ggataaattt
    10921 cccctacact ttaatagtga aaatttatcc ctcttggtaa ccagatttat gaaatttttt
    10981 aaa
//
LOCUS       DAUDYX010000057        10906 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.058, whole genome shotgun sequence.
ACCESSION   DAUDYX010000057 DAUDYX010000000
VERSION     DAUDYX010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10906
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.058"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            66..1289
                     /locus_tag="VHD02_001612"
     CDS             66..1289
                     /locus_tag="VHD02_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEQ5498205.1"
                     /translation="MPSMKHILKSLSILLVGFLGGLIAIITFNNLYPHSPSKINSGKA
                     TTSNMVFNNTTNTTKAVKAVQNAVVSVINYQDNPSSSLSNPYTKLFGEGRSKENKDAE
                     LSIFSEGSGVIYRKDGNSAYVVTNNHVIDGAKRIEILMADGSKVVGELVGADTYSDLA
                     VVKISSDKIKTVAEFADSTKLNVGEVAIAIGSPLGTQYANSVTQGIVSSLSRTVTLKN
                     ENGETVSTNAIQTDAAINPGNSGGPLINIEGQVIGINSSKISSTPTGSNGNSGAVEGI
                     GFAIPSTDVIKIIKQLETNGEVIRPALGISMVNLNDLSTNALSQINIPTSVTGGIVVA
                     EVKEGMPASGKLAQYDVITEIDGKTVNSISDLQSSLYGHDINDTIKVTFYRGTTKKKA
                     DIKLTKTTQDLTKTQ"
     gene            1348..2154
                     /locus_tag="VHD02_001613"
     CDS             1348..2154
                     /locus_tag="VHD02_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEQ5498206.1"
                     /translation="MFLILSRNSLMTKELLIDLPIEDIVTNPYQPRIQFNQRELQDLA
                     TSIKSNGLIQPIIVRKSDIFGYELVAGERRLKASKMAGLKKVPAIIKKISTLESMQQA
                     IVENLQRSNLNAIEEAKAYQLLVEKKHMTHDEIAKYMGKSRPYISNTLRLLQLPAPII
                     KTIEEGKISAGHARALLTLSDDKQQLYLTHKIQNEGLSVRQIEQLVTSTPSSKLSKKT
                     KNIFATSLEKQLAKSLGLSVNMKLTANHSGYLQISFSNDDELNRIINKLK"
     gene            2387..3742
                     /gene="dnaA"
                     /locus_tag="VHD02_001614"
     CDS             2387..3742
                     /gene="dnaA"
                     /locus_tag="VHD02_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEQ5498207.1"
                     /translation="MTENEQIFWNRVLELAQSQLKQATYEFFVHDARLLKVDKHIATI
                     YLDQMKELFWEKNLKDVILTAGFEVYNAQISVDYVFEEDLMIEQNQTKINQKPKQQAL
                     NSLPTVTSDLNSKYSFENFIQGDENRWAVAASIAVANTPGTTYNPLFIWGGPGLGKTH
                     LLNAIGNSVLLENPNARIKYITAENFINEFVIHIRLDTMDELKEKFRNLDLLLIDDIQ
                     SLAKKTLSGTQEEFFNTFNALHNNNKQIVLTSDRTPDHLNDLEDRLVTRFKWGLTVNI
                     TPPDFETRVAILTNKIQEYNFIFPQDTIEYLAGQFDSNVRDLEGALKDISLVANFKQI
                     DTITVDIAAEAIRARKQDGPKMTVIPIEEIQAQVGKFYGVTVKEIKATKRTQNIVLAR
                     QVAMFLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMISQDESLRIEIETIKNKI
                     K"
     gene            3897..5033
                     /gene="dnaN"
                     /locus_tag="VHD02_001615"
     CDS             3897..5033
                     /gene="dnaN"
                     /locus_tag="VHD02_001615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEQ5498208.1"
                     /translation="MIQFSINRTLFIHALNATKRAISTKNAIPILSSIKIEVTSTGVT
                     LTGSNGQISIENTIPVSNENAGLLITSPGAILLEASFFINIISSLPDISINVKEIEQH
                     QVVLTSGKSEITLKGKDVDQYPRLQEVSTENPLILKTKLLKSIIAETAFAASLQESRP
                     ILTGVHIVLSNHKDFKAVATDSHRMSQRLITLDNTSADFDVVIPSKSLREFSAVFTDD
                     IETVEVFFSPSQILFRSEHISFYTRLLEGNYPDTDRLLMTEFETEVVFNTQSLRHAME
                     RAFLISNATQNGTVKLEITQNHISAHVNSPEVGKVNEDLDIVSQSGSDLTISFNPTYL
                     IESLKAIKSETVKIHFLSPVRPFTLTPGDEEESFIQLITPVRTN"
     gene            5108..5305
                     /locus_tag="VHD02_001616"
     CDS             5108..5305
                     /locus_tag="VHD02_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEQ5498209.1"
                     /translation="MYQIGSFVEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            5635..6750
                     /gene="ychF"
                     /locus_tag="VHD02_001617"
     CDS             5635..6750
                     /gene="ychF"
                     /locus_tag="VHD02_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEQ5498210.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASRGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNILQKIKPVLEDGKSARTIEFTEDEAKVVKGLFLLTTKPVLYVANVDEGKV
                     ANPDGIDYVKQIRDFAATENAEVVVISARAEEEISELDDEDKEEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LMAYGSEKAVKEAGRLREEGKEYVVQDGDIMEFRFNV"
     gene            6820..7389
                     /locus_tag="VHD02_001618"
     CDS             6820..7389
                     /locus_tag="VHD02_001618"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEQ5498211.1"
                     /translation="MVKMIVGLGNPGSKYEKTKHNIGFMAIDNIVKNLDVTFTDDKNF
                     KAQIGSTFINHEKVYFVKPTTFMNNSGIAVKALLTYYNIDITDLIVIYDDLDMEVSKL
                     RLRSKGSAGGHNGIKSIIAHIGTQEFNRIKVGIGRPLKGMTVINHVMGQFNTEDNIAI
                     SLTLDRVVNAVKFYLQENDFEKTMQKFNG"
     gene            7392..10895
                     /gene="mfd"
                     /locus_tag="VHD02_001619"
     CDS             7392..10895
                     /gene="mfd"
                     /locus_tag="VHD02_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEQ5498212.1"
                     /translation="MNILELFSQNKKVQSWHSGLTTLGRQLVMGLSGSSKALAIASAY
                     LDDQKKIVVVTSTQNEVEKLASDLSSLLDEELVFQFFADDVAAAEFIFASMDKALSRI
                     ETLQFLRNPKSQGVLIVSLSGLRTLLPNPDVFTKSQIQLTVGEDYDSDTLTKQLMTIG
                     YQKVSQVISPGEFSRRGDILDIYEITQELPYRLEFFGDDIDSIRQFHPETQKSFEQLE
                     GIFINPASDLIFEASDFQRGIEQLEKALQTAQDDKKSYLEDVLAVSKNGFKHKDIRKF
                     QSLFYEKEWSLLDYIPKGTPIFFDDFQKLVDKNARFDLEIANLLTEDLQQGKALSNLN
                     YFTDNYRELRHYKPATFFSNFHKGLGNIKFDQMHQLTQYAMQEFFNQFPLLIDEIKRY
                     QKNQTTVIVQVESQYAYKRLEKSFQDYQFRLPLVSANQIVSRESQIVIGAISSGFYFA
                     DEKLALITEREIYHKKIKRRARRSNISNAERLKDYNELAVGDYVVHNVHGIGRFLGIE
                     TIQIQGIHRDYVTIQYQNSDRISLPIDQIGSLSKYVSADGKEPKINKLNDGRFQKTKQ
                     KVARQVEDIADDLLKLYAERSQQKGFSFSPDDDLQRAFDDDFAFVETEDQLRSIKEIK
                     ADMESMQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHYENFKA
                     RFENYPVEVDVLSRFRSKKEQAETLERVRKGQIDIIIGTHRLLSKDVVFSDLGLIVID
                     EEQRFGVKHKETLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLENNPGLVREAIIREMDRGGQIFYVYNKVDTIEKKVSELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFIENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIASKQGKTTVRQKGNTEINLQIDAYLPDDYISDERQK
                     IDIYKRIREIQSREDYLNLQDELIDRFGEYPDQVAYLLEIALLKHYMDNAFAELVERK
                     NNQVIVRFEVTSLTYFLTQDYFEALSKTHLKAKISEHQGKIDIVFDVRHQKDYRILEE
                     LMLFGERLSEIKIRKNNSVFK"
BASE COUNT         3763 a         1745 c         2003 g         3395 t
ORIGIN      
        1 ttatttttaa ggtataatta agcaaagttc tataagctat ggacaaataa ttcggaggac
       61 aaaacatgcc aagtatgaaa catatcttaa aatccttaag tattttacta gttggatttt
      121 taggaggatt aatagcaatt attacattca ataatctcta cccacattct ccttcaaaaa
      181 taaattcagg taaggcaaca actagtaata tggtttttaa caacacaacc aatactacta
      241 aagctgtcaa agctgttcaa aatgcagttg tatcagttat taattatcaa gataaccctt
      301 cttcatccct ttctaaccct tatacaaaac tctttggaga agggcgttca aaagagaata
      361 aggatgctga attatctatt tttagtgaag gatctggggt catttatcga aaagatggca
      421 actccgctta cgttgttact aataaccatg ttatcgacgg agctaaacgg attgaaattc
      481 ttatggcaga cggatctaaa gttgttggtg aattagttgg agctgatact tattcggatt
      541 tagctgttgt taagatctct tcagataaga taaaaacagt agctgaattt gcagactcta
      601 caaaactaaa tgttggagaa gttgctattg ctatcggcag cccactagga acacaatacg
      661 ctaattctgt tactcaagga atcgtctcta gtttaagtcg tactgtaact ttaaaaaacg
      721 agaatggtga gactgtctca acaaatgcta ttcagacaga tgctgctatt aaccctggaa
      781 actctggtgg accactaatt aatattgagg ggcaagtaat cggaattaat tcaagtaaaa
      841 tttcttctac cccaactggt agcaacggta atagtggtgc tgttgaagga attggttttg
      901 ctatcccatc tactgacgtt attaaaatta ttaaacaact agaaactaat ggtgaagtta
      961 ttagacctgc tcttggaata tccatggtca atttaaatga cttatccaca aatgctctta
     1021 gccaaattaa tattccaact agtgtaactg gtggtattgt agtagcagaa gttaaggaag
     1081 gaatgccggc atctggcaaa cttgctcagt acgatgtgat tacagaaatt gatggcaaaa
     1141 cagttaattc aattagtgat ttacaaagta gtctatacgg acacgatatt aatgatacta
     1201 ttaaagtaac tttttataga ggaactacaa agaaaaaagc agatatcaaa ttaacaaaaa
     1261 ctactcaaga tttgactaaa acgcagtaaa caagctacct aaatactatt acattttatc
     1321 acataaaaca gtccaaagga ctgttttatg tttcttattc tatccaggaa ctcccttatg
     1381 acaaaagaat tattgataga cctaccaatt gaagatattg ttacaaatcc ctatcagcca
     1441 aggatacaat ttaatcaaag agaactccaa gacctagcaa cttctattaa atcaaacggc
     1501 ctcattcaac ctattattgt tcgaaaatct gacatttttg gctatgagtt agttgctgga
     1561 gaacgacgac ttaaagcttc aaaaatggct ggtctaaaaa aagttccagc tatcatcaag
     1621 aagatctcta cactcgagag catgcaacaa gctatagttg aaaatttaca acgttctaac
     1681 cttaacgcta tcgaagaagc taaagcctat cagttattgg ttgaaaaaaa acacatgact
     1741 cacgatgaga ttgctaaata tatgggaaaa tcaagacctt atattagcaa caccttacgt
     1801 ctgttacaac tcccagcacc tatcattaaa acaattgaag aaggaaaaat tagtgcggga
     1861 cacgcgcgtg ctcttttaac tttgagtgat gataagcaac aactgtacct cactcataaa
     1921 atacaaaatg aaggcctaag tgttaggcaa attgagcaac tggttacttc tactccaagt
     1981 tcgaagctat ctaaaaaaac taaaaatatt tttgccactt ctttagagaa acaattggct
     2041 aaatcattgg gactctctgt caatatgaag ctgacagcaa accatagtgg gtaccttcag
     2101 atatcttttt ccaatgatga tgaattaaac agaattatca acaagctaaa gtagcttgtt
     2161 gatattctgt tttttctttt ttagttttcc acatgaaaaa tagttgaaaa caatagcggt
     2221 gtcaccttaa aatggctttt ccacaggttg tggagaaccc aaattaacag tgttaattta
     2281 ttttccacag gttgtggaaa aactaactat tatccatcgt tctgtggaaa actagaatag
     2341 tttatgatag aatagttcta gaattatcca caagaaggaa cctagtatga ctgaaaatga
     2401 acaaattttt tggaacaggg tcttggaatt agctcagagt caattaaaac aggcaactta
     2461 tgaatttttt gttcatgatg cccgtctatt aaaggtcgat aagcatattg caactattta
     2521 cttagatcaa atgaaagaac tcttttggga aaaaaatctt aaagatgtta ttcttactgc
     2581 tggttttgaa gtttataacg ctcaaatttc tgttgactat gttttcgaag aagacctaat
     2641 gattgagcaa aatcagacca aaatcaatca aaaacctaag cagcaagcct taaattcttt
     2701 gcctactgtt acttcagatt taaactcgaa atatagtttt gaaaacttta ttcaaggaga
     2761 tgaaaatcgt tgggctgttg ctgcttcaat agcagtagct aatactcctg gaactaccta
     2821 taatcctttg tttatttggg gtggccctgg gcttgggaaa acccatttat taaatgctat
     2881 tggtaattct gtactattag aaaatccaaa tgctcgaatt aaatatatca cagctgaaaa
     2941 ctttattaat gagtttgtta tccatattcg ccttgatacc atggatgaat tgaaagaaaa
     3001 atttcgtaat ttagatttac tccttattga tgatatccaa tctttagcta aaaaaacgct
     3061 ctctggaaca caagaagagt tctttaatac ttttaatgca cttcataata ataacaaaca
     3121 aattgtccta acaagcgacc gtacaccaga tcatctcaat gatttagaag atcgattagt
     3181 tactcgtttt aaatggggat taacagtcaa tatcacacct cctgattttg aaacacgagt
     3241 ggctattttg acaaataaaa ttcaagaata taactttatt tttcctcaag ataccattga
     3301 gtatttggct ggtcaatttg attctaatgt cagagattta gaaggtgcct taaaagatat
     3361 tagtctggtt gctaatttca aacaaattga cacgattact gttgacattg ctgccgaagc
     3421 tattcgcgcc agaaagcaag atggacctaa aatgacagtt attcccatcg aagaaattca
     3481 agcgcaagtt ggaaaatttt acggtgttac cgtcaaagaa attaaagcta ctaaacgaac
     3541 acaaaatatt gttttagcaa gacaagtagc tatgttttta gcacgtgaaa tgacagataa
     3601 cagtcttcct aaaattggaa aagaatttgg tggcagagac cattcaacag tactccatgc
     3661 ctataataaa atcaaaaaca tgatcagcca ggacgaaagc cttaggatcg aaattgaaac
     3721 cataaaaaac aaaattaaat aacatgtgga aaagaatatc ttttatgaaa tagttatcca
     3781 caagttgtga acaaccattt agtcttggat tctctcgttt atttagagtt atccactata
     3841 tacacaagac ctactactac tacttattat tatacttatt aaataaagga gttctcatga
     3901 ttcaattttc aattaatcgc acattattta ttcatgcttt aaatgcaact aaacgtgcta
     3961 ttagcactaa aaatgccatt cctattcttt catcaataaa aattgaagtc acttctacag
     4021 gagtcacttt aacagggtct aacggtcaaa tatcaattga aaacactatt cctgtaagta
     4081 atgaaaatgc tggtttgcta attacctctc caggagctat tttattagaa gctagttttt
     4141 ttattaatat tatttcaagt ttgccagata ttagtataaa tgttaaagaa attgaacaac
     4201 accaagttgt tttaaccagt ggtaaatcag agattacctt aaaaggaaaa gatgttgacc
     4261 agtatcctcg tctacaagaa gtatcaacag aaaatccttt gattttaaaa acaaaattat
     4321 tgaagtctat tattgctgaa acagcttttg cagccagttt acaagaaagt cgtcctattt
     4381 taacaggagt tcatattgta ttaagcaatc ataaagattt taaagcagta gcgactgact
     4441 ctcatcgtat gagccaacgt ttaatcactt tggacaatac ttcagcagat tttgatgtgg
     4501 ttattccaag taaatctttg agagaatttt cagcagtatt tacagatgat attgagaccg
     4561 ttgaggtatt tttctcacca agccaaatct tgttcagaag tgaacacatt tctttttata
     4621 cacgcctctt agaaggaaat tatcccgata cagaccgttt attaatgaca gaatttgaga
     4681 cggaggttgt tttcaatacc caatcccttc gccacgctat ggaacgtgcc ttcttgattt
     4741 ctaatgctac tcaaaatggt actgttaagc ttgagattac tcaaaatcat atttcagctc
     4801 atgttaactc acctgaggtt ggtaaggtaa acgaggattt agatattgtt agtcagtctg
     4861 gtagtgattt aactatcagc ttcaatccaa cttaccttat tgagtcttta aaagctatta
     4921 aaagtgaaac agtaaaaatt catttcttat caccagttcg accattcacc ctaacaccag
     4981 gcgatgagga agaaagtttt atccaattaa ttacaccagt acgaacaaac taagtaagaa
     5041 aaagctccct tttaggagtt tttttgttat tataaatatt aacgataata taagtggaga
     5101 aaagggaatg tatcaaattg gatcatttgt tgaaatgaaa aaacctcatg cttgtgtaat
     5161 taaagaaact ggtaaaaagg ctaatcaatg gaaggtactg agagtaggag ctgatattaa
     5221 aattcagtgt actaactgtc agcatgtgat tatgatgagt cgttatgatt ttgagcgaaa
     5281 attaaaaaaa gtactgcaac catgaaagtc cttttaaata aataattcta gctatatttg
     5341 caaccaatac tttcctaaaa aattgctagt atgccgttgg aaaattagct attctaatgt
     5401 tatcgaaaga agaaaggtgg ccattgacaa tgacgaaagt tgtagcacag ttacttagtt
     5461 tctcggcaaa ctatatcaaa atgagaataa tacaccaaac tttgataatt taaatgaagg
     5521 tgaaagagtg gcataatgat aagcaaatct gaaattttct tcttattcat accgcttttt
     5581 cttctatttt ttggtataat aatcttgatt gaaatttgaa tggagattcg ctaaatggct
     5641 ttaacagcag gtattgtagg cttaccaaat gttggtaaat caaccttatt taacgcaatt
     5701 acaaaagcag gagcagaagc tgctaattat ccttttgcga ctattgatcc caatgttggt
     5761 atggtagagg ttccagatga gcgtctgcaa aaattgacag aattgattac acctaaaaaa
     5821 acagttccga caacctttga atttactgat attgcaggta ttgttaaagg tgcttctaga
     5881 ggggaaggtc taggaaataa atttttagct aatattcgtg aagtcgatgc tattgttcat
     5941 gtggtacgtg cttttgatga tgaaaatgtc atgcgtgaac aaggtcgtga ggatgctttc
     6001 gttgatccta tagcagatat tgacactatt aatcttgaat taatcttagc tgatttagag
     6061 tcaatcaata aacgttatgc gcgtgttgaa aaaatggcac gaactcaaaa agataaagaa
     6121 tcagtagcag agttcaatat tttgcaaaag attaaacctg ttttggaaga tgggaaatca
     6181 gctaggacaa ttgagtttac agaagatgaa gcaaaagttg ttaaaggtct ctttttatta
     6241 acaactaaac ctgttttata tgtagctaat gtcgatgaag gtaaagttgc taatccagat
     6301 ggtattgatt atgtcaaaca aattcgtgac tttgcagcta ctgaaaatgc tgaagtagtt
     6361 gttatctcag cgcgtgcaga agaggaaatt tcagagcttg acgatgagga taaagaagaa
     6421 tttttggaag ctatcggtct tactgaatca ggcgttgata aattaaccag agcagcttat
     6481 catctcttag gccttggaac ctattttaca gcaggtgaaa aagaggttcg tgcttggacg
     6541 tttaagcgtg gtataaaagc tccacaagct gctggtatta tccattcaga ttttgaaaga
     6601 ggttttattc gtgcagtaac catgtcttat gatgatctaa tggcctacgg ttcagaaaaa
     6661 gccgtcaaag aagctggacg cttgcgtgaa gaaggaaaag aatatgttgt ccaagatggg
     6721 gacatcatgg aattcagatt taatgtgtaa ttatattaaa acaatatcag aaggttggaa
     6781 gaacattcca gcccttttgg cattttagaa agagaaaata tggtaaaaat gattgttggt
     6841 ctgggaaatc caggctctaa atatgaaaaa acaaagcaca atattggttt tatggctatt
     6901 gacaatattg tcaagaacct tgacgttacc tttacagatg ataaaaattt taaagcacag
     6961 ataggaagta cttttattaa tcatgaaaaa gtttactttg tgaaacctac tacttttatg
     7021 aataatagcg gcatagcagt aaaagcatta ctaacctact ataatattga cataacagat
     7081 ttaattgtta tctatgatga tttagacatg gaagtcagta aattacgttt acgtagtaag
     7141 ggttcagcag gaggacataa tggcattaag tcaatcattg cccacattgg aactcaggaa
     7201 tttaaccgaa tcaaagttgg tattggacga cctttaaaag gtatgactgt tattaaccat
     7261 gtgatgggcc aattcaatac cgaagataat attgctattt cgttaactct tgacagagtt
     7321 gtcaatgctg tcaagtttta tttacaagaa aatgattttg aaaaaacaat gcagaaattt
     7381 aatggataat catgaatatt ttagaattat ttagtcagaa taagaaagtc caatcctggc
     7441 actctggatt aaccacctta ggaagacaac tggtaatggg gttatcgggt tcaagtaaag
     7501 cattggctat agcttccgct tatttagatg atcaaaaaaa aatagttgtg gttacatcaa
     7561 ctcaaaatga ggttgaaaaa ttagccagcg atttatctag tttacttgat gaagaacttg
     7621 ttttccaatt ttttgcagac gatgtggctg cagcggaatt tatctttgcg tcaatggata
     7681 aagctctatc aagaatagaa accctgcaat ttttaaggaa tcctaaatct cagggcgttt
     7741 taattgttag tttatcaggc ttaagaactt tattgccaaa cccagatgtt tttacaaaga
     7801 gtcagattca actaacagtt ggagaagatt atgatagtga tactcttact aaacaactga
     7861 tgacaattgg atatcagaag gtctcacagg ttattagtcc gggagaattt agccgtcgag
     7921 gggatatttt agatatctat gagattacac aagaattgcc ttatcgattg gaattttttg
     7981 gtgatgatat tgatagtatt aggcaatttc atccagaaac tcaaaaatct tttgaacaac
     8041 tagaaggtat ttttattaat ccagcaagtg atcttatttt tgaggctagt gattttcaac
     8101 gtggcattga gcaattagaa aaggctctac aaacagcaca agatgataaa aaatcttatt
     8161 tagaagatgt attagctgtt tcaaaaaacg gttttaaaca taaggatatc cgtaaatttc
     8221 aatcattatt ttatgaaaaa gagtggtcat tattagatta tattcctaag ggaacgccaa
     8281 tcttttttga tgattttcaa aaactagttg ataaaaatgc aagatttgat ttagagattg
     8341 ctaatctctt gacagaagat ttacagcaag gaaaggctct ttccaatctt aactacttta
     8401 cagataatta tcgagagctt aggcactata agccagcgac cttcttttca aattttcata
     8461 agggacttgg aaatatcaaa tttgatcaga tgcatcagct aactcagtat gccatgcagg
     8521 aattttttaa tcaatttcct ttgttgattg atgagattaa acggtatcaa aaaaatcaaa
     8581 caacggttat tgtacaggta gagtctcagt atgcttacaa acgacttgaa aaatcttttc
     8641 aagattacca atttcgcctt cctttagtga gtgctaatca aattgtttca cgtgaatcac
     8701 aaattgtaat tggagctatc tccagtggtt tttattttgc tgatgaaaag ttagcgttaa
     8761 tcacagagcg tgaaatttat cataaaaaga tcaaacgacg cgctagacga tctaatatta
     8821 gcaatgctga gcgtttgaaa gattacaatg agttagcagt aggtgattac gtggttcata
     8881 atgtccatgg tatcggtcgc tttcttggaa ttgaaacgat tcagattcag ggaatccatc
     8941 gagattatgt tactattcaa tatcaaaatt cagaccgtat ttctcttcca attgaccaaa
     9001 ttggtagctt atcaaagtac gtttctgctg atggaaaaga acctaaaatt aataaactca
     9061 atgatggtcg tttccaaaag acaaagcaaa aggtcgctag acaagtagag gatattgctg
     9121 atgaccttct aaaattatat gctgaaagaa gtcagcaaaa aggattttca ttttcaccag
     9181 atgatgactt gcagcgcgct tttgatgatg attttgcttt tgtagaaaca gaagatcaac
     9241 ttaggtctat aaaggaaatt aaagctgata tggagagcat gcagcctatg gatcgtcttt
     9301 tagtaggcga tgtaggattt ggtaagacag aagtagctat gagggcagcc tttaaagcgg
     9361 tgaatgatca caaacaagta gctgtcttag ttccaaccac agtcttggcc cagcagcatt
     9421 atgaaaactt caaagcacgc tttgaaaatt accctgttga ggttgacgtc ttaagtcgtt
     9481 tccgtagtaa aaaagagcaa gctgaaacac tagaacgtgt acgaaaaggt caaattgaca
     9541 ttattatcgg aacccatcga ctcctatcaa aagatgtggt cttttctgat ttaggattga
     9601 ttgtgattga tgaggaacaa cggtttggtg ttaagcataa agaaacttta aaggaattaa
     9661 aaactaaggt tgacgtctta accttaacag ctactccgat tcctagaacc ttacacatgt
     9721 ctatgttagg tatccgagat ttatcggtta ttgagacccc accaaccaat cgttatcctg
     9781 ttcaaaccta tgttttggaa aataatccag gtctcgttag agaagctatc attcgtgaaa
     9841 tggatcgtgg aggacaaatt ttttacgttt acaataaagt tgacactatt gaaaagaaag
     9901 tttcagagct acaagaatta gtcccagaag cttctatagg ttttgttcat gggcaaatga
     9961 gtgaaattca acttgaaaat accttgattg actttataaa tggtgattat gatgtccttg
    10021 tggctacaac aatcatcgaa acaggagttg acatttctaa tgtaaacact ttgtttattg
    10081 agaatgctga tcatatggga ttgtcaactt tatatcaact acgagggcgc gtcggaagaa
    10141 gtaatcgtat tgcttatgct tacctgatgt atcgtcctga taaggtccta acagaggttt
    10201 ctgaaaagcg tttagaggct attaaaggct ttactgaatt aggttcaggc ttcaagattg
    10261 ctatgcgaga tttgtctata cgaggagcag gtaatatttt aggggcttct cagagtggct
    10321 ttattgattc ggtcggtttt gaaatgtatt ctcagttatt ggaacaggct attgctagca
    10381 agcaaggaaa aacgactgtt cgccaaaaag gtaatactga aatcaatctt cagattgacg
    10441 cctatttgcc agatgattac atatcagatg agcgtcaaaa aattgacatt tacaagcgta
    10501 ttcgagaaat tcaatcaaga gaagattatc tcaatttgca ggatgagctg atagatcgtt
    10561 ttggagagta tcctgatcaa gttgcctatt tgttagagat agctctgcta aaacattata
    10621 tggacaatgc ctttgcggaa ttagttgaac gcaaaaataa tcaggtcatt gttcgatttg
    10681 aagtaacttc tttaacttat ttcttgactc aggattattt cgaagcctta tcgaaaaccc
    10741 atcttaaagc taaaattagt gaacatcagg ggaaaatcga tatcgtcttt gacgttcgcc
    10801 atcaaaaaga ttatagaatt ttagaagaat tgatgttatt tggagaaagg cttagtgaga
    10861 taaaaatcag aaaaaacaat tcagttttta aataataatt tgaaac
//
LOCUS       DAUDYX010000058        10425 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.059, whole genome shotgun sequence.
ACCESSION   DAUDYX010000058 DAUDYX010000000
VERSION     DAUDYX010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10425
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.059"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            188..616
                     /gene="lacA"
                     /locus_tag="VHD02_001620"
     CDS             188..616
                     /gene="lacA"
                     /locus_tag="VHD02_001620"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEQ5498213.1"
                     /translation="MAIIIGADKAGQELKEVIKDYLKEGKYEVVDVSENEVRDFVDTT
                     LAVAKEVNASEDNLGIVIDAYGVGSFMVATKIKGMVAAEVSDERSAYMTRGHNNARII
                     TLGSEISAPGIAKNIIKGFVEGKYDGGRHQVRVDMLNKMC"
     gene            651..1166
                     /gene="lacB"
                     /locus_tag="VHD02_001621"
     CDS             651..1166
                     /gene="lacB"
                     /locus_tag="VHD02_001621"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEQ5498214.1"
                     /translation="MKIAVGCDHIVTYEKIAVVDYLKSQGHKVIDCGTYDNVRTHYPI
                     FGKKVGEAVASGEAELGVVICGTGVGITNAVNKVPGIRSALVRDMTSAIYSKEELNAN
                     VIGFGGKIIGGLLMNDIIDAFLAAEYKPTEENKKWIEKMDSLQHASQDQNNPHFFDEF
                     LEKWDRGEYHD"
     gene            1212..2141
                     /locus_tag="VHD02_001622"
     CDS             1212..2141
                     /locus_tag="VHD02_001622"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEQ5498215.1"
                     /translation="MILTVTLNPAIDVSYPLDELKCDTVNRVVDVTKTPGGKGLNVSR
                     VLNDFGETVKATGCVGGESGDFIINHPPDSILSRFYKISGDTRTCIAILHEGNQTEIL
                     EKGPLLSVDEIDGFTHQFKYLLNDVDVVTMSGSLPAGMPDDYYQKLIKIANLNGKKTV
                     LDCSGNALEAVLKGDSKPTVIKPNLEELSQLLGKEMTKDFEALKEVLQDELFEGIEWI
                     IVSLGADGVFAKHKDTFYNVDIPKIKIVSAVGSGDSTVAGIASGLANDEDDRALLTKA
                     NVLGMLNAQEKTTGHVNMANYDKLYQSIKVKEV"
     gene            2145..3128
                     /gene="lacD"
                     /locus_tag="VHD02_001623"
     CDS             2145..3128
                     /gene="lacD"
                     /locus_tag="VHD02_001623"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEQ5498216.1"
                     /translation="MTITLTENKRKSMEKLSIDGVISALAFDQRGALKRMMAQHQTKE
                     PTVEQIEELKSLVSEELTPFASSILLDPEYGLPASRVRSEEVGLLLAYEKTGYDATTT
                     SRLPDCLDVWSAKRIKEAGAEAVKFLLYYDIDGDQDINEQKKAYIERIGSECRAEDIP
                     FYLEILTYDEKISDNASPEFAKVKAHKVNEAMKVFSEERFGVDVLKVEVPVNMKFVES
                     FADGEVLFTKEEAAQAFRDQEASTDLPYIYLSAGVSAKLFQDTLVFAAESGAKFNGVL
                     CGRATWAGSVKVYIEEGPQAAREWLRTEGFKNIDELNKVLDKTASPWTEKM"
     gene            3152..3469
                     /locus_tag="VHD02_001624"
     CDS             3152..3469
                     /locus_tag="VHD02_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEQ5498217.1"
                     /translation="MNREETTLLGFEIVAYAGDARSKFLEALKAAQEGDYAKAEELIA
                     AGSDCLNDAHNAQTSLLQKEAAGDDLAYSVTLMHGQDHLMTTILLQDLMKHMIELYKR
                     GAK"
     gene            3469..5166
                     /locus_tag="VHD02_001625"
     CDS             3469..5166
                     /locus_tag="VHD02_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEQ5498218.1"
                     /translation="MNGLIAQIEKGKPFFEKMSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIESLLMTPYNYSMGILGLFVAGTTAKALTDSMNRSLPSTNQ
                     INFMSTMLAAIVGFLLMAANPAKEGGFLTGFMGTKGLLTAFIAAFITVNVYKTCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTLSVVLLYVIELIVHSTLGVNVAESIGKLLAPLFQ
                     AADGYVGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAETNLQLLQAGEHADKIL
                     TSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNKAIGRASVVPTFFGVNEPILFGAPL
                     VLNPIFFIPFILAPIVNVWIFKFFIDTLGMNSFTANLPWTTPAPLGLILGTNFQVLAF
                     ILAALLIVVDVIIYYPFLKIYDEQILAEEAAGTNSSDALKEKVAANFDTKKADAILEK
                     SAAKETKEITDQTNVLVLCAGGGTSGLLANALNKAAKEYGAPVTAAAGSYGAHREILP
                     QYQLVILAPQVASNYEDMKVETDKLDIKLAKTEGAQYIKLTRDGQGALDFVKEQMEK"
     gene            5239..6645
                     /gene="lacG"
                     /locus_tag="VHD02_001626"
     CDS             5239..6645
                     /gene="lacG"
                     /locus_tag="VHD02_001626"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEQ5498219.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLSEEFGVNGIRISIAWSRIFPTGKGEVNPKGVEYYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVNYAELCFKEFSEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLFKDSGYSGEIGVVHAL
                     PTKYPFDANNPDDVRAAELEDIIHNKFILDATYLGKYSDKTMEGVNHILEVNGGELDL
                     REEDFAALDAAKDLNDFLGINYYMSDWMQAFDGETEIIYNGKGEKGSSKYQIKGVGRR
                     KAPVDVPKTDWDWIIFPQGLYDQIMRVKADYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVISDAISDGANVKGYFMWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAYWYKKVAETQVIE"
     gene            7148..7303
                     /locus_tag="VHD02_001627"
     CDS             7148..7303
                     /locus_tag="VHD02_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A2 lantipeptide"
                     /protein_id="HEQ5498220.1"
                     /translation="MSFMKNSKDILTNAIEEVSEKELMEVAGGKKGSGWFATITDDCP
                     NSVFVCC"
     gene            7385..9010
                     /locus_tag="VHD02_001628"
     CDS             7385..9010
                     /locus_tag="VHD02_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4135 domain-containing protein"
                     /protein_id="HEQ5498221.1"
                     /translation="MIKRNELKLEYQQHQFYEYFNSIFDYDILDTSISENIYLLREKT
                     HKLFYSEFENQLFETIMFLSMKTLVLDINHFSKEIENKSEAYEQCIQQIREENGINHF
                     FDRYPYLLKQINKEVGLIEESYSLLFDRFLEDLSEIRSCFNISEPLSNVAFSLGDSHS
                     KKQTVVKIAFKEKSVYYKPKSYHSHSILLELTSLLKSSNIPSFSLPKSLVKADYCWQL
                     GVAYTSSNKDEVAKIYFKYGVLAAFSEIFSITDLHMENVIVSGGDLYLIDVETFFQRK
                     LNVQNQNFEGITVDTYQRIYETSLSNGLFPVQFEKNSAPNVSGISGKGGKRKKGKYEL
                     INKNRGDMKLVKTDYFQEDGYNIPTLNGKVVEPLDYANEVIAGFRECYTFLMSQRAKV
                     KKILEDFPKLKTRAIFRNTSDYGKFLQASTNPKYLFSEKKRENLFSILHESKHIEQFI
                     VVSEIKDLMNGDIPYFSMDTRGNVYNSVGTLIGNLGDTTSLFDSITTLNDERLKFTCE
                     LLEIVLKKPIKHWEREKGKSYQFLSISSEHYLL"
     gene            9012..9545
                     /locus_tag="VHD02_001629"
     CDS             9012..9545
                     /locus_tag="VHD02_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5498222.1"
                     /translation="MDKTDFRNISAFVSQDSPIFDGDVMYNISLGRESVSGEQVIETC
                     KRVSLYEDIRSMPMKFHTPLFRDNPSLSGGQKQRISLARELVTTPRILVLDEPTSALD
                     VKTERIIQKNVEALHCTRVLVTHRLNTVEKADKILIMDNGKIIDYGSHHCLYKNNEYY
                     RDLYDSYMNNYQEEEIK"
     gene            9542..10279
                     /locus_tag="VHD02_001630"
     CDS             9542..10279
                     /locus_tag="VHD02_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5498223.1"
                     /translation="MNQLLLKNVCKRYPNQPNYALDHINLTVRKGEFVAVMGRSGSGK
                     TTLLNVTSIIDKIDSGSIYCADKEISAFSDNEATSFRKNDIGFVFQDYMLLDSLTIRE
                     NISVALSLKNVDSSKINDLINNYAKRFNLYEQLEKYPYQLSGGQRQRVSIIRAIIKEP
                     EIIFADEPTGALDLKSSEGTMRILSEINKTEKVTILMVTHDVLSASYADRVVLLKDGK
                     LHMEIDKEDCGETFYDVISQALSDRGE"
BASE COUNT         3512 a         1577 c         2067 g         3269 t
ORIGIN      
        1 aaaataaaca tattttgttt attttttata aaaataaaca aaaaaatgtt gacttttatg
       61 tttggtgggt gtatgattag gtcatagaaa taatgaaagc gttatttcaa aagatgttgc
      121 aaatcgttaa ttaactatct acgtaggttt tccacattta cttattctaa atcataggag
      181 aaataaaatg gcaattatta tcggtgctga caaagcaggt caagaactta aagaagttat
      241 caaagattac cttaaagaag gtaaatacga agtcgttgat gttagtgaaa acgaagtacg
      301 tgactttgta gatacaactt tagccgttgc aaaagaagtt aatgcctccg aagataactt
      361 aggtattgtc attgatgcat atggtgttgg ttctttcatg gtagctacta aaatcaaagg
      421 aatggtggca gctgaagtat cagatgagcg ctcagcatac atgacaagag gacacaacaa
      481 tgctcgaatc atcacgcttg gttctgaaat tagtgctcca ggaattgcaa agaacattat
      541 caaaggcttt gtcgaaggta aatatgatgg tggacgtcat caagttcgtg tcgacatgct
      601 taataaaatg tgctaattga ttgaaatagg ttaaaacaag gagaacaaaa atgaaaattg
      661 ctgtaggttg tgaccatatt gttacttatg aaaaaatcgc tgttgtggat tacctaaaat
      721 cacaaggtca taaagttatt gattgtggta cgtatgataa tgttcgcaca cactatccaa
      781 tttttggtaa aaaagttggt gaagcagtag caagtggtga agcagaacta ggtgttgtta
      841 tctgtggtac tggtgttggt attacaaatg ccgtaaacaa agttcctgga attcgttcag
      901 cactcgttag agatatgaca tctgctatct actcaaaaga agagctaaac gcaaacgtta
      961 ttggttttgg tggaaaaatc atcggtggtt tattgatgaa tgatattatc gatgctttct
     1021 tagccgcgga atacaaacca actgaagaaa ataaaaaatg gattgaaaaa atggatagct
     1081 tgcaacatgc tagccaagat caaaacaacc cacacttctt tgatgaattt ctagaaaaat
     1141 gggaccgtgg tgaatatcac gactaatatt tgaaagaatt atgataagga tatatagaaa
     1201 aaggagctat tgtgatttta acagttactt taaatccagc tattgacgtc tcctaccctt
     1261 tggatgagct aaaatgcgat actgtcaatc gagttgtaga cgttacaaag acacccggtg
     1321 gtaaaggctt gaatgtttcc cgagttctaa atgactttgg tgagacagtc aaagctacag
     1381 gttgtgtagg tggagaaagc ggtgatttta tcattaatca tcctcctgat tcgattttaa
     1441 gtcgcttcta taaaatttca ggtgatacta gaacttgtat agccatttta cacgaaggta
     1501 atcaaacgga gattttagaa aaaggaccgc tgttaagtgt agatgaaatt gatggattta
     1561 ctcatcaatt taagtattta ctgaatgatg ttgacgtagt gacaatgtca ggcagtttgc
     1621 ctgctggtat gccagatgat tactatcaga agttaatcaa gattgccaat cttaatggta
     1681 agaaaacggt tctggattgt tctggaaatg ctctagaagc agtattgaaa ggtgatagta
     1741 aaccaacagt tatcaagcct aatcttgaag aactttctca acttttaggt aaagaaatga
     1801 caaaagattt tgaagcctta aaagaagtct tacaagatga actatttgaa ggaattgaat
     1861 ggataatcgt ttctcttggt gctgatggtg tatttgcaaa gcacaaggat acattttata
     1921 acgttgacat tcctaaaatc aaaattgtaa gcgctgtagg ttcaggggat tctacagtgg
     1981 caggtattgc ttcaggttta gcgaatgatg aagatgatcg agctttatta acaaaagcaa
     2041 atgtcttagg gatgttaaat gcgcaagaaa aaacaactgg gcatgttaac atggcaaatt
     2101 acgataagtt atatcaatct attaaagtca aagaggtata aaaaatgaca attactttaa
     2161 ctgaaaacaa acgtaaaagc atggaaaaac taagtataga tggtgttatt tctgctttag
     2221 cttttgacca acgtggagct ctcaaacgta tgatggctca acatcaaaca aaagagccaa
     2281 cagtagaaca aattgaagag cttaaaagtt tagtttctga agaactaaca ccatttgcat
     2341 catcaatttt acttgatcct gaatatggct tgccagcaag tcgtgttcgt tcagaagaag
     2401 taggcttgtt gttggcatat gagaaaacag gatacgatgc aacaacaact agtcgcttac
     2461 ctgattgctt agatgtttgg tctgcaaaac gtattaaaga agctggagca gaagcagtta
     2521 aattccttct ttactatgat attgatggag atcaagatat caacgagcag aaaaaagctt
     2581 acattgaacg tattggttct gaatgccgtg cagaagatat tccattctac ttagaaattc
     2641 taacttatga tgagaaaatc tctgacaatg ctagccctga gtttgcaaaa gttaaagctc
     2701 ataaagttaa tgaagcaatg aaagtcttct cagaagaacg ctttggtgtt gacgtcttga
     2761 aagtcgaagt tccagtaaat atgaaatttg tagaaagctt tgcagatggt gaggttctct
     2821 ttacaaaaga agaagcagct caagctttcc gtgatcaaga agcttctact gatttaccat
     2881 atatttactt gagtgcaggt gtttctgcca aattgttcca agatactttg gtgtttgcag
     2941 cagaatcagg tgctaaattc aacggtgtct tatgtggacg tgctacctgg gcaggatctg
     3001 taaaagtcta tatcgaagaa ggaccacaag cagctcgtga atggttgcgt acagaaggtt
     3061 tcaaaaatat tgacgaatta aacaaagtgc tagataaaac tgcaagtcct tggactgaaa
     3121 aaatgtaaca ttattttcgg aggaaactac aatgaataga gaagaaacta ctcttttagg
     3181 ttttgaaatc gtagcctatg ctggtgatgc ccgttcaaaa tttttggaag cgcttaaagc
     3241 agcgcaagaa ggagattatg ctaaagcaga agaattaatc gctgcaggta gtgactgctt
     3301 gaatgatgct cacaatgctc agacaagtct actacaaaaa gaagcagctg gagatgactt
     3361 agcttatagt gtcactttaa tgcacggtca agatcattta atgacgacca ttttattaca
     3421 agatttaatg aaacacatga ttgaacttta taagagagga gcaaaataat gaatggatta
     3481 attgctcaga ttgagaaagg gaaacctttc ttcgagaaaa tgtctcgaaa tatctatctt
     3541 cgcgctattc gtgatggttt tatcgctggt atgccagtta tcttgttttc aagtatcttt
     3601 atcttgattg cctatgttcc aaatgcatgg ggcttccatt ggagtaagga tattgagagt
     3661 ctcttgatga cgccatacaa ctactcaatg ggtattttag gtttgtttgt ggctggtacg
     3721 acagccaaag ctttaactga ctcaatgaat cgatcattac caagcacaaa ccaaatcaac
     3781 ttcatgtcta ccatgctcgc tgctattgta ggttttttac ttatggcagc caatccagca
     3841 aaagaaggtg gtttcttaac tggatttatg ggaacaaaag gtttgttgac agccttcatc
     3901 gcagctttta ttaccgtaaa tgtctataaa acctgtgtga aaaacaatgt cactattcgc
     3961 atgccagaag aggtgccacc aaacatctct caagttttta aagatttgat tccatttacg
     4021 ttatcagttg tactgcttta tgtgattgaa ttgattgttc atagtacttt aggcgttaac
     4081 gttgccgagt caattggtaa acttcttgca ccattgttcc aagcagcaga tggctatgtt
     4141 ggaatcacta ttatcttcgg tgcatttgct ttcttctggt ttgttggtat tcatggacca
     4201 tctattgttg aaccagccat tgcagcgatt acttacgcaa atgctgaaac caaccttcaa
     4261 ttacttcaag ctggtgaaca tgctgacaag attttgactt cagggactca aatgttcatc
     4321 gttaccatgg gtggtactgg tgcaactctc gtcgttccat tcatgttcat gtggttaaca
     4381 aaatcaaaac gtaacaaagc cattggacgt gcatcagttg ttccaacatt ctttggtgtt
     4441 aacgaaccca ttttgtttgg tgctccactt gttttgaacc caatcttctt tattccattt
     4501 atcttggcac caattgtcaa tgtatggatt ttcaaattct tcattgacac acttggaatg
     4561 aacagtttca ctgcaaactt accttggaca acaccagctc cattgggtct tatccttggt
     4621 acaaacttcc aagtgctagc attcattttg gcagctttgt taatcgtagt tgatgtcatc
     4681 atctactacc cattcttgaa aatttatgat gagcaaattc ttgctgaaga agcagcaggt
     4741 acgaattcat cagatgccct caaagaaaaa gtagcagcaa actttgatac taaaaaagct
     4801 gatgctattc ttgaaaaatc agcagctaaa gaaaccaaag aaatcactga tcaaactaat
     4861 gttctcgttc tttgtgccgg tggtggtaca agtggtttgc ttgcaaacgc tttgaataaa
     4921 gcagctaaag aatatggtgc acctgtcact gcagcagcag gaagttatgg tgctcaccgt
     4981 gagattttac cacaatatca acttgttatc cttgcacctc aagtagcttc taactatgaa
     5041 gatatgaaag tggaaactga taaacttgat attaaattag ctaaaacaga aggggctcaa
     5101 tacatcaaat tgactcgcga tggtcaaggc gcattagact tcgttaaaga acaaatggaa
     5161 aaataatatg tcgctaggga aggttggggg tactcaatct tcctttatag tgaaatgaaa
     5221 atttagaaga ggtatatcat gactaaaaca ttacctaaag attttatttt tggtggtgct
     5281 acagctgctt accaggctga aggcgctacc cacacagatg gtaaaggacc agtagcttgg
     5341 gataaatact tagaagacaa ctattggtac acagctgagc cagcaagtga tttttataat
     5401 cgttaccctg tcgatttgaa acttagtgaa gaatttggtg ttaatgggat ccgtatctct
     5461 attgcctggt ctcgtatttt tccaacagga aaaggagaag ttaaccctaa aggagtagaa
     5521 tactaccaca atctttttgc agagtgtcat aagcgtcatg ttgagccttt tgttacactt
     5581 caccattttg atacaccaga agcacttcat tcaaatggtg atttcctcaa tcgtgagaac
     5641 attgaacatt ttgtaaatta tgcagaactc tgttttaaag aattctcaga agttaactat
     5701 tggacaacct tcaacgaaat tggaccaatt ggcgatggtc aatacttggt tggtaaattc
     5761 cctccaggta tccaatatga tcttgctaaa gttttccaat cacatcataa catgatggtc
     5821 tctcatgctc gtgcagtcaa actctttaaa gatagtggtt attcaggtga aattggtgtt
     5881 gtccatgctc ttccaactaa gtatccattt gacgctaaca atcctgatga tgttagagca
     5941 gctgaactgg aagatatcat ccataataaa tttattcttg atgcaactta tctaggtaag
     6001 tattcagata aaacaatgga aggtgttaac catatccttg aggtgaatgg tggtgaactt
     6061 gatcttcgtg aagaagattt tgctgcacta gacgctgcaa aagatttgaa tgacttcctt
     6121 ggtattaact actatatgag tgattggatg caagcttttg atggtgagac tgaaatcatt
     6181 tacaatggta agggtgaaaa aggcagctct aaataccaaa tcaagggtgt tggtcgaaga
     6241 aaagcacccg ttgatgttcc aaaaacggac tgggactgga ttatcttccc acaaggctta
     6301 tatgatcaaa tcatgcgtgt caaagccgat tatcctaact acaagaaaat ttacattaca
     6361 gagaatggtc ttggctacaa agatgagttt gtagataata ctgtctatga tgatggacgt
     6421 atcgattatg tgaaaaaaca cttagaagtt atttctgatg ctatttctga tggtgcaaat
     6481 gttaaaggat actttatgtg gtcactgatg gatgtctttt catggtcaaa tggctatgaa
     6541 aaacgttacg gtctcttcta tgtcgatttt gaaactcaag aacgttatcc taagaagagt
     6601 gcctactggt ataaaaaagt agcagaaact caagtgattg aatgattttg agatactttt
     6661 ttaatacatt tttacacttc atgttcgttt actgaattat agtcacaaaa agtagatgat
     6721 gttgacattt ttcaagcata gtattatttg ctgttgatgg taatccatta gttgtaagat
     6781 cgggttataa gtgtttttag ttgatgaaaa tgggttttga aagttttatt taacatcctt
     6841 tttcagatat ttgacttctg tcattcaaac tcctgacctg attaaataag taaagtaacc
     6901 attttctgat tagagattga tgttataacg agaagcgatt agattgtaaa atagctgtaa
     6961 agaacgagtt ttgtttttta tatcagaggg ctatttgtta taaagtgacc tcttatgtga
     7021 catctcaaaa atcttactaa aaaatatgaa aaaagtcatt tttgcacttt ttactcattg
     7081 aaaatgaaga cgtattattt acaataaaag tgtagaaaat agtcactaca tactgatagg
     7141 aaggagaatg agttttatga aaaattcaaa ggatattttg actaatgcta tcgaagaagt
     7201 ttctgaaaaa gaacttatgg aagtagctgg tggtaaaaaa ggttcaggtt ggtttgcaac
     7261 tattactgat gactgtccga actcagtatt tgtttgttgt taattaaaac aatctggata
     7321 agcagactaa aatttcctgt ttagacttag tctaggcagg atttttaagt agtggtagga
     7381 aaaaatgata aaaagaaatg aattaaagtt agagtatcag caacatcagt tttacgaata
     7441 ctttaactca atttttgatt acgacatatt agatacttct attagtgaaa atatttattt
     7501 gttaagggaa aagacacata agttatttta tagtgagttt gaaaatcagt tatttgaaac
     7561 gatcatgttc ctttcgatga aaacactagt tttagatata aatcattttt ccaaggaaat
     7621 tgaaaataag tcagaggcat atgagcagtg tatacagcag ataagagaag aaaacggaat
     7681 aaatcatttt tttgacaggt atccgtattt gctaaaacaa attaataaag aagtaggact
     7741 gatagaggaa tcatattctt tattatttga tagattttta gaagacttat ctgagataag
     7801 gtcgtgcttt aacattagtg agcccctaag caatgttgcc ttttcacttg gtgacagtca
     7861 ctctaaaaag caaacagttg ttaaaattgc gtttaaagag aaatctgttt actacaagcc
     7921 taaaagttat cattcacaca gtatcttgtt agaacttact agtttattga aatctagtaa
     7981 tataccttca ttcagtcttc ctaaaagctt agttaaagca gattattgtt ggcaacttgg
     8041 tgtagcttat acaagttcca ataaagatga agtagcgaaa atttacttta agtatggtgt
     8101 tttagcagca ttttctgaaa tattttctat tacggactta catatggaaa atgtgattgt
     8161 ttcaggcgga gatttatatt taattgacgt tgaaacattt tttcaaagaa agttgaatgt
     8221 acaaaatcag aattttgaag ggattactgt agacacctat cagagaattt atgagacatc
     8281 actttcaaac ggtttatttc ctgtacaatt tgagaaaaac agcgctccta acgtttctgg
     8341 aataagtgga aaaggtggaa aaagaaaaaa gggaaagtat gaactgataa ataaaaatcg
     8401 tggagatatg aagttagtga aaactgacta ttttcaggaa gatggttata atattccgac
     8461 attaaatggg aaagtggtgg aacctctcga ctatgctaat gaggtaattg caggttttag
     8521 agaatgctat acatttctca tgtcgcaacg tgcaaaggtt aaaaaaattt tagaagattt
     8581 cccaaaattg aaaactagag ccatattcag aaatacttcc gactatggaa aatttttaca
     8641 agcctctacg aatccaaaat atcttttctc agaaaaaaag agggagaatt tattttcgat
     8701 actacatgag tcaaaacata ttgaacaatt tattgttgtt agtgaaatta aagatttgat
     8761 gaatggtgat attccttact tttcaatgga taccagagga aacgtctata attctgtagg
     8821 gacattaatt ggaaatttag gagatacaac ctcgttattt gatagtataa ctactttgaa
     8881 cgatgaaaga ttgaagttca cttgtgaatt gcttgaaata gtcttgaaaa aaccgattaa
     8941 gcattgggag agggaaaagg gcaaatctta tcagttttta tcaatctcct cagaacatta
     9001 ccttctctaa aattgataaa actgatttta ggaatatatc ggcttttgta tctcaagatt
     9061 ctcccatatt tgatggggat gtgatgtata acatttcgct agggagagaa tctgtttcag
     9121 gagaacaggt tattgaaact tgtaaaaggg tatcactata tgaggatatc aggagtatgc
     9181 caatgaagtt tcatacccca ctttttcgag acaatccatc actatctggg gggcaaaaac
     9241 aacgaatttc tctagcaaga gagttagtaa ctacccctag aatcttagtt cttgatgaac
     9301 ctacatcagc tttagatgta aaaactgaaa gaataatcca aaaaaatgtt gaggctttac
     9361 attgtacgag ggttttggtt acccatagac ttaatacagt tgaaaaagct gataagattt
     9421 taataatgga taatgggaaa attattgact atggtagtca tcattgttta tataaaaata
     9481 atgagtacta tcgtgattta tatgattcgt acatgaacaa ctatcaggag gaagagataa
     9541 aatgaatcaa ttactattga aaaatgtttg caaaaggtat ccaaaccaac cgaattatgc
     9601 tttagaccat atcaatttga ctgttagaaa aggagaattt gtagctgtca tgggacgtag
     9661 tggtagtgga aaaacaacac ttttaaatgt tacttcaatt attgataaaa ttgatagtgg
     9721 gagtatttat tgtgcagaca aagaaattag tgcattttct gataacgagg caactagttt
     9781 tagaaaaaat gatattggtt ttgtttttca agattatatg ctgttagaca gtctaaccat
     9841 tagggagaat atttctgttg ctctatcttt aaaaaatgtt gattcatcaa aaattaatga
     9901 tttgataaat aactatgcaa aaagatttaa tttatatgag cagttggaaa aatatccgta
     9961 tcaactttct ggaggtcaaa gacagagggt cagtattatc agagcaatca ttaaagaacc
    10021 agagattatt tttgctgacg aaccaactgg agcacttgac ttaaaatctt ctgaaggaac
    10081 aatgaggatt ttgagtgaaa tcaataaaac agaaaaggta actattttaa tggttactca
    10141 tgacgtttta tcagcttctt atgcggatag agttgtactt ctcaaagatg gtaaacttca
    10201 catggagatt gataaagagg attgtggaga gacgttttat gatgtaatta gtcaagcctt
    10261 atccgataga ggagagtaat atgatttggt ccattacaaa atctaacatc aaaaaaaact
    10321 tttcgttata tcgtatctat tttctagcta cgattggttt attaagtatt tttatagctt
    10381 ttctaaattt tatctcagat aaaatcatta cagaaaaaat tgggg
//
LOCUS       DAUDYX010000059         9916 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.060, whole genome shotgun sequence.
ACCESSION   DAUDYX010000059 DAUDYX010000000
VERSION     DAUDYX010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9916
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.060"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(152..2416)
                     /gene="glgP"
                     /locus_tag="VHD02_001631"
     CDS             complement(152..2416)
                     /gene="glgP"
                     /locus_tag="VHD02_001631"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEQ5498224.1"
                     /translation="MTRFTEYVETKLGKSLTQASNEEIYLSLLNFVKEEASHKAKNSA
                     KRKVYYISAEFLIGKLLSNNLINLGIYKDIKEELAAAGKSIAEVEDVELEPSLGNGGL
                     GRLASCFIDSISSLGINGEGVGLNYHCGLFKQVFKHNEQEAEPNYWIEDDSWLVPTDI
                     SYDVPFKNFTLKSRLDRIDVLGYKRDTKNYLNLFDIEGVDYGLIKDGISFDKTQIAKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAVQLIIDEAIERGSNLHDLADYAYVQINDT
                     HPSMVIPELIRLLTEKHGFDFDEAVAVVKNMVGYTNHTILAEALEKWPTAYLNEVVPH
                     LVTIIEKLDALVRSEVSDPAVQIIDESGRVHMAHMDIHFATSVNGVAALHTEILKNSE
                     LKAFYDLYPEKFNNKTNGITFRRWLEFANQDLADYIKELIGDEYLTDATKLEKLMAFA
                     DDKAVHVKLAEIKFNNKLALKRYLKDNKDIELDEHSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKKGNLPKRKITVIFGGKAAPAYIIAQDIIHLILCLSELINNDPEVSPYLNV
                     HLVENYNVTVAEHLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGMENIYTFGKDSDTIINLYATASYVAKDYYDNHPAIKAAVNFIISPELLAFGNEE
                     RLDRLYKELISKDWFMTLIDLEEYIEVKEKMLADYEDQDLWMTKVVHNIAKAGFFSSD
                     RTIEQYNEDIWHSR"
     gene            complement(2451..3944)
                     /gene="malQ"
                     /locus_tag="VHD02_001632"
     CDS             complement(2451..3944)
                     /gene="malQ"
                     /locus_tag="VHD02_001632"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEQ5498225.1"
                     /translation="MNKRASGILMHISSLPGKFGIGTFGKSAFEFVDLLAETKQTYWQ
                     ILPLTTTSFGDSPYQSFSAIAGNTHFIDFELLVDDELLEAADLCDITFGTNPEAVDYA
                     QLFQVRRPLLEKAVRAFVAEQENVCKLEAFETASSWLTDFAEFMALKEYFNNKALQDW
                     DDETVIKRQEDSLNNYRELLAKKITYHKVCQYFFYQQWSALKTYANHKGIEIIGDMPI
                     YVSADSVEVWTMPELFKVDSDKKPLFIAGVPADGFSEDGQLWGNPTYNWSAHEKSNFA
                     WWIYRIQESFKLYDQLRIDHFKGFSDFWEIPAGDKTARNGHWASAPGIALFSAVREAL
                     GELPIIAENLGYIDEKAEQLLASTGFPGMKILEFGLFDITSQSIDLPHYYDRNCVAYT
                     GTHDNEVVNGWYDNLSEGQVHFVNNYLHKHADESITKAMLRTIFASVCDTAILCIQDL
                     LDKDGKSRMNMPNTIGGNWQWRMLDGELNQDHKDYLIYLTDLYGRAR"
     gene            complement(4058..5077)
                     /locus_tag="VHD02_001633"
     CDS             complement(4058..5077)
                     /locus_tag="VHD02_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ5498226.1"
                     /translation="MVTIKDVAQKAGVNPSTVSRVLKDNRSISMKTKEKVRKAMADLG
                     YVPNVAAQILASGLTHNIGLVFPPITTSDRLSEPFFMEILSTITNEAKESHFTVSIAT
                     GISLDDLIEQVKLMHLQKRVDGFIILYSDQDDPVKKYLMTNNLPFVIVGAPQGDTNKT
                     TYIDNDNQLMAKTAVEHLYQKGHHNILFITDDLLSEVTSERYLGYLKGCFKRSLKTKP
                     MLLFDRKDPVSVEILMETISSFKATALVVVGDVLAIRMVQLLSFYDIKVPDDMSIITF
                     NNSNYAKLIHPYLTTFDINVENLGKMSFKQLLDIINSNEQNLSQTIFVPFSLKQRESV
                     RDISP"
     gene            5321..6568
                     /locus_tag="VHD02_001634"
     CDS             5321..6568
                     /locus_tag="VHD02_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ5498227.1"
                     /translation="MKSWQKVIVGGASLTLASTLLVGCGSGSKDKKEAGADSKTIKLW
                     VPTGSKKSYADTIAKFEKDSGYTVKVVESEDPKAQEKIKKDASTAADVFSLPHDQLGQ
                     LVESGTIQEVPEKYNKEIAATSTDQALIGAQYKGKTYAFPFGIESQVLFYNKSKLAAE
                     DVTSYDTITTKATFGGTFKQANTYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAV
                     LKWIADQASNKGFVSLDANNVMSKFGDGSVASFESGPWDYEAAQKAIGKENLGVAIYP
                     KVTIGGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLASYLTNAESQENQFKTRN
                     IVPANKEVQSSEAVQSNELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFN
                     GKIKESDYLTKLQQFDKDIAATK"
     gene            6842..8203
                     /locus_tag="VHD02_001635"
     CDS             6842..8203
                     /locus_tag="VHD02_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ5498228.1"
                     /translation="MTQSNLTPNISVIEALKRGSWDIKLSSIIMGFANFANKQFIKGI
                     LFLISELIFLVAFVSQIIPAIRGLVTLGTQTQGMTTKTIDGINIQVAVDGDNSMLMLI
                     FGLASLIFCLVFAYIYWCNLKSARNLYLFKQKGQKIPSFKEDLATLTNGRFHMTLMAI
                     PLIGVLLFTILPLIYMICLAFTNFDHNHLPPKSLFDWVGLANFGNVLSGRMAGTFFPI
                     FSWTLIWAVFATVTNFFFGIILALLINTKGLKWKKMWRTIFVITIAVPQFISLLIMRN
                     LLNDEGPLNALLNKIDLINGSLPFLSDPLWAKFSIIFVNMWIGIPFTMLIATGIIMNL
                     PSEQIEAAEIDGASKFQVFKSITFPQILLIMTPNLIQQFIGNINNFNVIYLLTGGGPT
                     NSEYYQAGTTDLLVTWLYKLTVTAADYNLASVIGILIFTVSAIFSLLAYTRTASYKEG
                     AAK"
     gene            8203..9039
                     /locus_tag="VHD02_001636"
     CDS             8203..9039
                     /locus_tag="VHD02_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ5498229.1"
                     /translation="MKNKRRFQLGLVYATLIILSIIWLFPIAWVILTSFRSEGTAYVN
                     YFIPKTFTLNHYINLFTNETFPFGKWFMNTLIVATFTCIISTFITVAIAYSLSRIKFK
                     FRNGFLKLALILNMFPGFMSMIAIYYILKALGLTQTLTALVLVYSSGAALGFYIAKGF
                     FDTIPYSLDESAMIDGATRMDIFFKITLPLAKPIIVYTALLAFMGPWIDFIFAQVILG
                     DATSKYTVAIGLFSMLQPDTINNWFMAFTAGSVLIAVPITLLFMFMQKYYVEGITGGS
                     VK"
     gene            9226..9916
                     /locus_tag="VHD02_001637"
                     /pseudo
     CDS             9226..9916
                     /locus_tag="VHD02_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871253.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         3044 a         1949 c         1708 g         3215 t
ORIGIN      
        1 cctccataga tcgctaaaca gacttgcttt acgcttaatg taataattaa cctaagtctg
       61 aagtaaaatc ataagacagc agaaactact gtcttatgat ttagtttaag catgttacgg
      121 taccgctacc ccacattgca attattattt tttatcgtga atgccaaata tcttcgttat
      181 actgctcaat ggtacggtca gatgagaaga atccagcttt tgcgatgtta tggaccactt
      241 ttgtcatcca taaatcttgg tcttcatagt ctgctaacat tttttctttc acttcaatgt
      301 actcttcaag gtcaatcaaa gtcatgaacc agtcttttga aatcaattct ttataaagac
      361 gatcaagacg ttcttcgttg ccaaatgcta gcaattctgg actgataata aagttcactg
      421 ctgctttaat agcaggatgg ttatcatagt aatcttttgc tacataagaa gcagtcgcat
      481 aaaggttgat gatggtgtca gaatctttac caaaggtgta gatattctcc atgcctgcaa
      541 gctcagcgat ttctacgtta gcaccgtcca ttgtaccaag tgttaaagca ccgttaagca
      601 tgaatttcat attaccagtt ccagaagctt ctttggatgc tagtgaaatt tgctcagaaa
      661 tatcagttgc tggaatcaag tgctctgcta ctgtcacgtt gtaattttca actagatgca
      721 cattaaggta tgggcttact tcagggtcat tgttaatcaa ttcagacaag caaaggatca
      781 agtgaatgat gtcttgagca atgatgtaag caggcgctgc tttacctcca aagataacag
      841 tgattttacg ttttggaagg ttgccttttt taatttccaa atatttgtga atcacgtaaa
      901 gagcattcat ctgttgacgt ttgtactcgt ggaaacgttt gatttgggta tcaataatag
      961 aatgttcatc aagctcaatg tctttattgt ctttaaggta acgtttaaga gctaatttgt
     1021 tgttgaattt gatttcagcc aatttaacat gaacagcttt gtcatctgca aaggccatca
     1081 atttttctaa ttttgttgcg tcagtcaagt actcatcgcc aataagttct ttgatgtaat
     1141 cagccaagtc ttggttagca aattctagcc aacgacggaa agtaatcccg ttggttttgt
     1201 tgttgaattt ttctgggtaa aggtcataga aagcttttaa ttcgctgttt ttcaagattt
     1261 ctgtgtggag tgctgctacc ccattgacac ttgttgcaaa atgaatatcc atatgggcca
     1321 tgtgcacacg acctgattcg tcaataattt gaacagctgg gtcagacact tctgaacgaa
     1381 caagagcatc caatttttca atgatggtta ccaagtgtgg cactacttcg tttaagtaag
     1441 cagttggcca tttttcaagg gcttctgcaa gaatagtgtg gttagtgtaa ccaaccatat
     1501 ttttcacaac agctaccgct tcatcaaagt caaagccatg tttttcagtc aagagacgaa
     1561 ttaattctgg gatgaccatt gatggatgcg tgtcattgat ttgcacgtaa gcgtagtctg
     1621 caaggtcatg aaggtttgaa ccacgttcga tagcttcatc aatgattaat tgcactgcat
     1681 ttgacaccat aaagtactgt tggtaaatac ggagcaattc cccatttttg tcagaatcgt
     1741 ctgggtagag gaacaaggtc aagtttttag caatttgcgt tttatcaaat gaaataccgt
     1801 ctttgattaa tccgtaatca acgccctcga tatcaaacaa gttaaggtag tttttagtgt
     1861 cgcgtttgta acccaaaaca tcaatacgat caagacgaga ttttaaggta aagtttttga
     1921 agggcacatc gtaagaaatg tctgttggaa ccaaccatga gtcatcttca atccagtagt
     1981 ttggctcagc ttcttgctca ttgtgtttga agacttgctt aaacaaccca caatgatagt
     2041 ttaaaccaac cccttcacca ttaataccaa gagatgaaat agagtcaatg aaacatgatg
     2101 ccaaacgtcc taaaccacca ttccctagtg atggttctaa ttcaacatct tcgacttctg
     2161 cgatagattt gccagcagct gccaattctt ctttgatgtc tttgtaaatt cctaggttaa
     2221 tcaggttgtt tgatagtaat ttaccaatca aaaactctgc tgagatatag taaactttgc
     2281 gtttagcaga atttttagcc ttgtggctag cttcttcttt gacaaagttt aataatgata
     2341 gataaatctc ttcattgctg gcttgcgtaa gtgattttcc tagtttagtt tcaacatatt
     2401 ctgtaaagcg tgtcatgtct tcctcttttc aataatgtaa catttaaata ttatcttgcg
     2461 cgaccataaa gatctgttaa ataaataagg tagtctttgt gatcttggtt caattcaccg
     2521 tcaagcatac gccattgcca attacctcca attgtatttg gcatattcat acgactctta
     2581 ccatctttat ctaataaatc ttggatgcaa agaatggctg tatcacaaac agaagcaaag
     2641 attgttctta gcatggcttt tgtaatactt tcatctgcat gtttgtgaag atagttattt
     2701 acaaagtgta cttgtccttc actaagattg tcataccatc cgttaaccac ctcgttatca
     2761 tgagtccctg tgtaagcgac acaattacgg tcatagtaat gtggcaaatc tatactttga
     2821 ctcgttatat caaataaacc aaattctaat atcttcatgc caggaaagcc tgttgatgct
     2881 aatagctgct ctgctttttc atcaatataa ccaaggtttt ctgcgatgat aggaagctct
     2941 cctaaggcct cacgaaccgc agaaaataac gctatacctg gggctgaggc ccaatggcca
     3001 tttctagcag ttttatcacc agcaggaatt tcccaaaaat ctgagaagcc tttaaaatga
     3061 tcgattctta gctgatcata tagtttgaaa ctttcttgaa tacgataaat ccaccatgca
     3121 aaattagact tttcatgagc agaccaattg taggttggat ttccccacag ctgaccatct
     3181 tcactaaagc catctgctgg tacacctgca ataaacaatg gctttttatc actatctact
     3241 ttaaatagct caggcatcgt ccaaacttct acactgtccg cagaaacata aataggcata
     3301 tcaccaataa tttctatccc tttatggtta gcgtaagttt tcaaggctga ccattgttgg
     3361 taaaagaagt attgacagac tttatgatag gtaatctttt ttgcaagcaa ctcacggtaa
     3421 ttattaagtg agtcttcttg gcgtttaatg acagtttcgt cgtcccaatc ttgtaaggct
     3481 ttattgttaa aatattcttt taaagccata aattcagcaa aatcagttaa ccagctagaa
     3541 gctgtttcaa aagcttctaa tttacacaca ttttcttgtt cagcaacaaa agctctcact
     3601 gctttttcta aaagtggacg cctaacttga aaaagctgag catagtctac tgcttcagga
     3661 tttgtgccga atgtaatatc acacaaatct gctgcttcta aaagttcatc atctactagc
     3721 aattcaaaat caatgaaatg tgtgttccca gcaatagctg aaaatgactg ataaggagaa
     3781 tctccaaaac tcgtcgttgt taaaggcaga atttgccaat aggtttgttt cgtctctgct
     3841 agaagatcaa caaattcaaa agctgatttt ccaaaagtgc ctatcccaaa tttcccaggc
     3901 agggaactaa tatgcattaa gataccgctt gcacgtttgt tcatgatttt tctccttgtg
     3961 aaatctattc atgatgagat tataacgcaa gcgcttgcgt aagtcaacca gtttcctaaa
     4021 aaaagtaaca ttccttaaaa atgctacttt tttgttctta aggactaata tcacgaacgc
     4081 tttctctttg ttttaaacta aaaggaacaa aaatagtctg actaagattc tgttcattgg
     4141 aattgataat atcaagtaat tgcttaaaag acatttttcc aagattttca acatttatat
     4201 caaatgtcgt taaataaggg tgaattaatt tagcgtaatt agaattatta aaagtgataa
     4261 ttgacatatc atctggcact tttatatcat aaaaagataa taactgtacc atacgaatag
     4321 ccagaacatc accaacaaca accaaggcag ttgctttaaa ggaagatata gtttccatca
     4381 atatttctac gctaacagga tccttacgat caaataacag cattggcttc gtttttaaag
     4441 atcgcttaaa acaacccttc aaatagccaa gataacgctc agaagttact tctgatagta
     4501 aatcatctgt tataaataat atgttgtgat ggcctttttg ataaaggtgt tctactgctg
     4561 ttttagccat tagctgatta tcattatcaa tataagtggt tttattagtg tcaccttgtg
     4621 gagcacctac aatgacaaat ggtaagttat ttgtcatcaa atattttttg acaggatcat
     4681 cttgatcaga ataaagaata ataaaaccat caactcgttt ttgaagatgc atcagtttca
     4741 cttgctctat caggtcatca agagatatac ctgtagcaat cgacacagta aaatgagatt
     4801 ctttagcttc gttagtaata gtcgacaaaa tttccataaa gaaaggttca cttaatctat
     4861 cgctagttgt aataggagga aaaacaagtc ctatattgtg tgtcaaaccg ctcgctagaa
     4921 tctgtgctgc gacatttgga acatatccca aatctgccat cgcttttcta accttctctt
     4981 ttgtcttcat agaaatagat cgattatctt ttaataccct actcaccgtc gaagggttaa
     5041 cacctgcctt ttgagcgaca tcttttattg ttaccatatt aacctcgtta ctatatttac
     5101 caaagataaa ccttatcatg ggtttgtcat gtatttcgtt tccattatac aattatcttt
     5161 caaaaaatca acttcaatca agattccctt gtcattttaa ctcaaattct ctaaactatt
     5221 tttttaaaaa aacgaccttt ttttaaaaaa aggcttgact ttttctgcaa gcggttgcat
     5281 aatattaagt gtaagaaata tatcttagga ggaaatagct atgaaatcat ggcaaaaagt
     5341 tatcgtcggc ggagcaagtt tgacacttgc aagtaccttg ttagtgggat gtggatcagg
     5401 ttcaaaagat aaaaaggaag ctggcgctga ctcaaagact attaaacttt gggtaccgac
     5461 tggctctaaa aaatcatatg cagataccat tgcaaaattt gaaaaagatt ctggttacac
     5521 cgttaaagtg gttgaatcag aagatccaaa agcacaagaa aaaatcaaaa aagatgcctc
     5581 aactgctgcc gatgtctttt cacttcctca tgatcaactt ggtcaacttg tagaatctgg
     5641 tactattcaa gaagttccag aaaagtacaa taaagaaatt gctgctactt ctactgatca
     5701 ggcgcttatc ggtgcacagt acaaagggaa aacatatgct ttcccatttg gtattgaatc
     5761 acaagttctt ttctacaata aatcaaaatt agctgctgaa gacgttactt catatgatac
     5821 aattactaca aaagcaactt ttggtggtac ctttaaacaa gctaacactt acgctactgg
     5881 tccattattc atgtcagttg gtaacacttt atttggggaa aacggtgaag atgtaaaagg
     5941 tactaactgg ggcaatgaaa aaggcgctgc tgttcttaaa tggatcgcag accaagcttc
     6001 aaataaaggt tttgttagct tagatgctaa caacgttatg tctaaatttg gtgacggttc
     6061 agtagcttcc tttgaatcag gaccgtggga ctatgaagct gctcaaaaag ctatcggtaa
     6121 agaaaacctt ggtgttgcta tttatccaaa agtaactatt ggaggcgaaa cggttcaaca
     6181 aaaagcattc ttgggtgtaa aactttacgc agtcaaccaa gcaccagcta aaggtgatac
     6241 aaaacgtatc gcagctagct acaaattggc atcctacttg actaatgctg aaagccaaga
     6301 aaaccaattc aagactcgta acattgtccc agccaacaaa gaagttcaat cttcagaagc
     6361 tgttcaatca aacgaacttg ctaaaaccgt tatcactatg ggttcttctt cagactacac
     6421 tgtcgtaatg ccaaaactta gccaaatggg cacattctgg actgaaagtg ctgctattct
     6481 tagtgatgcc ttcaacggta aaatcaaaga aagcgactac cttactaagt tgcaacaatt
     6541 cgacaaagat atcgctgcaa caaaataata tgatctcatg tcatcactgc tatactaaaa
     6601 caagcacaag gtaagtactt ctaaaaagtt ctttccactt accttatgaa gagtggaact
     6661 aggctccatc acattttatt caagcttagt ctcactctta cttgtttttg ttttactagt
     6721 agcttagtgc tacttaaatt tttaactctt taaaagttta cgtaacactt agctatttca
     6781 tcctaacaag agattgaatc tgaactacca ctctagtatc ctaaaataaa ggagaatcta
     6841 tatgactcag tcaaacttga caccaaatat atctgtaata gaagccttaa aaagaggcag
     6901 ttgggatatt aaactttcga gtattatcat gggatttgcc aactttgcta acaaacagtt
     6961 tatcaaagga atcttatttt taataagtga acttatcttt ctagtagctt ttgtctccca
     7021 aatcatccct gctatccgtg gccttgtcac tcttggtact caaactcagg gaatgactac
     7081 aaaaactatt gatgggatta acatccaagt tgctgttgat ggcgataact ctatgctaat
     7141 gcttattttt ggtttagcat cactcatttt ctgcctagtc tttgcttaca tttactggtg
     7201 taatctcaaa agtgctcgta acctttatct tttcaaacaa aaaggtcaga agataccaag
     7261 cttcaaagaa gatcttgcca cacttacaaa cggtcgcttt catatgacct taatggctat
     7321 cccactgatt ggtgtgcttt tattcaccat tcttcctttg atttacatga tctgtttggc
     7381 ctttaccaat tttgatcata atcacttgcc tccaaaatct ttatttgatt gggtgggact
     7441 tgctaacttt ggaaatgttt taagcggccg tatggcagga accttttttc ctatcttctc
     7501 atggactttg atttgggctg tttttgccac agtaactaat ttcttctttg gtattattct
     7561 tgctttgtta atcaatacca aaggattaaa gtggaaaaaa atgtggcgaa ccatctttgt
     7621 tatcaccatt gcagttccac aattcatctc attattgatt atgcggaact tactcaatga
     7681 cgaaggccca ctaaatgctc ttctcaataa aatcgacttg attaatggct cactgccatt
     7741 tttatccgat cctctttggg caaaattctc aatcattttt gtcaacatgt ggatcggtat
     7801 tccatttacc atgttgattg cgactggtat tatcatgaac cttccaagtg aacaaattga
     7861 ggctgctgaa attgatggcg ctagcaaatt ccaagtcttc aagtctatca cgttccctca
     7921 aattctcttg atcatgacac caaacttgat tcaacaattt atcggaaata tcaataactt
     7981 taacgtcatt tacctcctta ctggtggtgg tccaacaaat tcagaatact atcaggcagg
     8041 aacaacagac ttgctggtca cttggctcta taaattaacc gtcacagctg ctgactacaa
     8101 tttagcttct gttatcggta tcttaatctt tacagtttca gctatcttta gcttacttgc
     8161 ttatacaagg acagcatcct acaaggaagg agcggctaaa taatgaaaaa caaacgtcga
     8221 tttcaacttg gacttgttta tgctacttta atcatcctct caatcatttg gctcttccca
     8281 atcgcttggg ttattcttac tagcttccgt agcgaaggaa ctgcttacgt caattatttt
     8341 attcctaaga cttttacatt aaatcactac attaatcttt ttactaatga aacctttcct
     8401 tttggaaaat ggtttatgaa tactcttatt gtagccactt ttacatgtat tatttcaacc
     8461 ttcataactg ttgccatagc ctactctctt agccgaatta agtttaagtt tcgcaatggc
     8521 tttttaaaac tagcccttat tttaaatatg ttccctggtt tcatgagcat gattgccatc
     8581 tactatatct taaaggcctt aggtttgacg caaaccctaa cagcacttgt ccttgtttat
     8641 tcctcaggtg cagcccttgg tttttacatt gcaaaaggat tttttgatac cattccttat
     8701 tcacttgatg aatcagctat gattgatggc gctactcgta tggatatttt ctttaaaata
     8761 acccttcctt tagcaaaacc aatcattgtc tatactgccc ttcttgcttt tatggggcct
     8821 tggattgact ttatctttgc acaagttatt ttaggtgatg ctacaagtaa atataccgtt
     8881 gctattggac tattttcaat gcttcagcca gataccatta acaactggtt tatggccttc
     8941 actgctggtt ctgtcttaat cgccgtccca atcactctcc tcttcatgtt tatgcaaaaa
     9001 tactatgttg aaggcatcac aggtggttct gttaaataag ttacaacata ccaaaccttc
     9061 taagggcaag ccctttagga ggtgtttgat aaataaggcc gtagataatc atattagtcc
     9121 tcacatttat cttcagatcc cgcgttgtaa aatgaagtgc aacaaaaaag acatgttcgt
     9181 ctgatatact agaattcccc aattcagtat ggaaagcaga tgaatatgtc taatattaat
     9241 tctaccagaa aatcctccta ttctcatctt tcagccacag aacggggtga aattgctgct
     9301 tatcttaaaa tgggaaagaa acccgttgag attgctcgac ttctgggccg tcaccgttct
     9361 acaatctgcc gagaaataaa acgtggttca gtagatcagg taaaggataa gaatggaaaa
     9421 caaaccttct tcaacgccta tttcgctgat agtggacaac gtgtctatga aaccaatcga
     9481 tttgcctaag atggttagta ttagaaaacg ttctaagaaa gtcatgaaaa cgaataagaa
     9541 gactttaggt aaatctatcg aagaacgtcc agaatatatt aatgatcgtt ctgaatttgg
     9601 acattgggag attgatttgg ttctcggcaa gaaaaccaaa ggtgaagctg ttatgttgac
     9661 tttggtagag cgccagacac gctatgcact aggagttaag ctagaagaca agcagtccca
     9721 aaccattaac cgtgctgtca cgcatctcat cagtcattat cctattgcat ccatcacagc
     9781 ggataatggt tctgagttta gtttactctc agacttaaaa gctgttgacg tttactttgc
     9841 acatccctat tcttcacatg agagaggaac aaacgagaac ttcaatggcc tcctcagaga
     9901 atatgtccct aaagga
//
LOCUS       DAUDYX010000060         9532 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.061, whole genome shotgun sequence.
ACCESSION   DAUDYX010000060 DAUDYX010000000
VERSION     DAUDYX010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9532
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.061"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            99..287
                     /locus_tag="VHD02_001638"
     CDS             99..287
                     /locus_tag="VHD02_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEQ5498230.1"
                     /translation="MLYIDEFKEAIDKGYILGDTVAIVRKNGKIFDYVLPHEKVRDDE
                     VVTVERVEEVMVELDYIK"
     gene            complement(439..786)
                     /gene="ssrA"
                     /locus_tag="VHD02_001639"
     tmRNA           complement(439..786)
                     /gene="ssrA"
                     /locus_tag="VHD02_001639"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            878..1492
                     /locus_tag="VHD02_001640"
     CDS             878..1492
                     /locus_tag="VHD02_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEQ5498231.1"
                     /translation="MTKRNSKAYILWQKIIKILGIIALIGTFFLAFWLYRLGILNDSN
                     ALKDLVQRYRLWGPFVFIVVQIIQIVFPVIPGGLTTVAGFLIFGPVTGFIYNYVGIII
                     GSIVLFLLVKTYGRKFILLFVNDKTFYKYERRLETPGYEKLFIFCMASPVSPADIMVM
                     ITGLTDMSLKRFVTILLITKPISIIGYSYLFIFGKDVISWFLQH"
     gene            complement(1625..2410)
                     /locus_tag="VHD02_001641"
     CDS             complement(1625..2410)
                     /locus_tag="VHD02_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ5498232.1"
                     /translation="MFGKLLKYEFRSIGKWYFALNAFVIAIAAILSFTIKLFAQSNSD
                     GLFGVLTNKMLPLTLGLTFGSLIAGSLLSTLLIIIKRFSKSVFGREGYLTLTLPVNSH
                     QIILSKLLASFICSVFNTIILAFAIAIVIVPMFNINELLEGFFNSFKVDYFINMLTVL
                     AYVLLSTFTSILLIYLSISIGQLFSNRRGLMAFIAYFILVILISVAATYVHSHIFNIN
                     TSAGSFPFTEQKTIYLLILEQFIEMIMFYLATNFIIKNKLNLQ"
     gene            complement(2420..3118)
                     /locus_tag="VHD02_001642"
     CDS             complement(2420..3118)
                     /locus_tag="VHD02_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5498233.1"
                     /translation="MAHLLQLHHVSKSYREKKAIDDLTITIPNGKIIGLLGPNGSGKT
                     TLIKLINGLLQPNKGEIVIDGYRPCVETKKIISYLPDTTYLNENMRIKDMLEFFSDFY
                     SDFDKSKATSLLRDLELDPEDRFKTLSKGNKEKVQLILVMSRKARLYVLDEPIGGVDP
                     AARDYILKTIINSYCENASVIISTHLISDIEPILDEVIFLKQGRLFLSGNADDLRQEY
                     QQSIDSLFRETYKV"
     gene            complement(3118..3489)
                     /locus_tag="VHD02_001643"
     CDS             complement(3118..3489)
                     /locus_tag="VHD02_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ5498234.1"
                     /translation="MSWKFDEKSPIYAQIAQHVMIQIISQEIKSGDQLPTVREYAEIA
                     GVNPNTMQRAFTELEREGMVYSQRTAGRFVTDDQKLIARKRRELAISELESFITNMTK
                     MGFSHTEIIPVLTSFLKEDSQ"
     gene            3674..6784
                     /locus_tag="VHD02_001644"
     CDS             3674..6784
                     /locus_tag="VHD02_001644"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEQ5498235.1"
                     /translation="MFAQLDTKTVYSFMDSLIDLNHYFERAKQFGYHTIGIMDKDNLY
                     GAYHFIKGCQKNGLQPVLGLEVEILYQERQVLLNLIAQNTQGYHQLLKISTAKMSGKL
                     HMDYLCQHLEGIAVIIPSKGWSDTLVVPFDYYIGVDQYTDLSHMDSKRQLIPLRTVRY
                     FAQDDMETLHMLHAIRDNLSLAETPVVESDQELADCQQLTAFYQTHCPQALQNLEDLV
                     SGIYYDFDTNLKLPHFNRDKSAKQELQDLTEAGLKEKGLWKEPYQSRLLHELVIISDM
                     GFDDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVAYALNITGIDPVQHDLLFERFLN
                     KERYSMPDIDIDLPDIYRSEFLRYVRNRYGSDHSAQIVTFSTFGPKQAIRDVFKRFGV
                     PEYELTNLTKKIGFKDSLATVYEKSISFRQVINSRTEFQKAFAIAKRIEGNPRQTSIH
                     AAGIVMSDDTLTNHIPLKSGDDMMITQYDAHAVEANGLLKMDFLGLRNLTFVQKMQEK
                     VAKDYGCQIDIAAIDLEDPQTLALFAKGDTKGIFQFEQNGAINLLKRIKPQRFEEIVA
                     TTSLNRPGASDYTTNFIKRREGQEKIDLIDPVIASILEPTYGIMLYQEQVMQIAQVYA
                     GFTLGKADLLRRAMSKKNLQEMQKMEEDFIASAKHLGRAEETARGLFKRMEKFAGYGF
                     NRSHAFAYSALAFQLAYFKAHYPAVFYDIMMNYSSSDYITDALESDFQVAQVTINSIP
                     YTDKIEASKIYMGLKNIKGLPRDFAYWIIEQRPFNSVEDFLTRTPEKYQKKVFLEPLI
                     KIGLFDCFEPNRKKILDNLDGLLVFVNELGSLFSDSSFSWVDTKDYSATEKYSLEQEI
                     VGVGMSKHPLIDIAEKSTQTFTPISQLVKESEAVVLIQIDSIRIIRTKTSGQQMAFLS
                     VNDTKKKLDVTLFPQEYAIYKDQLKEGEFYYLKGRIKERDHRLQMVCQQVQMAISQKY
                     WLLVENHQFDSQISEILGAFPGTTPVVIHYQKNKETIALTKIQVHVTENLKEKLRPFV
                     LKTVFR"
     gene            6864..7877
                     /gene="pfkA"
                     /locus_tag="VHD02_001645"
     CDS             6864..7877
                     /gene="pfkA"
                     /locus_tag="VHD02_001645"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEQ5498236.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGM
                     VDGDIFPLGSKEVGDKISRGGTFLYSARYPEFAQLEGQLAGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSH
                     GRTFVVEVMGRNAGDIALWAGIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHI
                     IVLAEGVMSGEAFAQKLKEAGDKSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVEL
                     LKEGKGGLAVGIHNEELVESPILGTAEEGALFSLTEEGKIIVNNPHKARLDFAALNRS
                     LSQ"
     gene            7940..9442
                     /gene="pyk"
                     /locus_tag="VHD02_001646"
     CDS             7940..9442
                     /gene="pyk"
                     /locus_tag="VHD02_001646"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEQ5498237.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAE
                     LIEAGANVFRFNFSHGDHKEQGDRMATVRLAEEIARQKVGFLLDTKGPEMRTELFADD
                     AKEFSYVTGEKIRVATTQGIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKV
                     IDKDIATRQFIVEVENDGIIAKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVEEVREICRETGNDHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVFQKMIITKVNAAGKAVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDRNAQTLLNEYGRLDSSAFPRTNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRALMINWGVIPVLAE
                     KPASTDDMFEVAERVAVEAGLVQSGDNIVIVAGVPVGTGGTNTMRVRTVK"
BASE COUNT         3041 a         1572 c         1938 g         2981 t
ORIGIN      
        1 aaaaaacatt aacttatatt aataatttta ttataaaata acaaaaaaac aatagttatt
       61 tttatctttt tacgaataga taggtaggag gatgaaatat gttatatata gatgagttta
      121 aagaagcgat tgataagggg tatattttag gtgacacagt agcgatagtg cgtaaaaacg
      181 gaaagatatt tgattatgtg ttaccacacg aaaaagtgag agatgatgaa gttgtgacgg
      241 tagagagagt ggaagaagtg atggtggaat tagactatat caaatgaaga aaactttgag
      301 tagacagaaa aataattgaa gagttgtcaa tttagttgtc aataatatag aaagttatgg
      361 taactcatga acaagacaaa aagaaaaaac cttgatgtaa caaggtttta gtaagttatg
      421 attacttacg gtaagcattg atggagctgg tgggagtcga acccacgtcc aaacacctgc
      481 caacatattt gtctacgtcc ataggttatg tcttcattta acaaactaaa gacacataac
      541 tcaagccatt agtttgcgag tctatcaatc tcttgtcacg aaactagaca atttcgtaac
      601 cgtagcttgc tattttgaga cttctaacag gacacaagca atcttgtaga agtcacacta
      661 gctggttttt aggcagctaa agcgtaagag tttgtatttt ttgcagttat atttaactgg
      721 caatttacat ctgccgatgg gacgcaaaac atgcctcata atgcctgtcg aatccgtaac
      781 aaccccaaat agataattaa ccaatttctt acactaacta ttctatcaaa acttttttta
      841 aatagcaaaa ttttacagct tatggtatga ttagtctatg acaaaacgta attcaaaagc
      901 ctatatatta tggcaaaaaa ttatcaaaat tttaggaatt attgcactaa ttggtacttt
      961 tttcctcgct ttttggctgt atagactagg tatattaaac gatagtaatg ctcttaaaga
     1021 cttagtgcag cgctatagat tatgggggcc gtttgttttt attgtcgtgc agatcataca
     1081 gattgttttt cctgtgattc ctggaggcct tacgacagtt gcaggttttt taatctttgg
     1141 ccctgtaaca ggttttattt ataattatgt aggcatcatt attggcagta tagtcctctt
     1201 cttgcttgtt aagacttatg gtcgtaaatt tatcttactc tttgtaaatg acaaaacatt
     1261 ttacaagtat gaaaggcgtt tggagacacc aggatatgaa aaattattta ttttttgtat
     1321 ggcatcgcct gtttctccag ctgatattat ggttatgatt acgggattga cggacatgtc
     1381 tttaaagcgc tttgtaacta ttcttttaat aactaaacca atttctatta tcggctacag
     1441 ttatttattt atttttggaa aagatgttat atcttggttc ttacagcatt aaggtttggt
     1501 gttaatttga taaaccgcgc ttctatttga tggaagacgg tttttttatt tactatgaac
     1561 actgaaaaaa actttctttt aaactatagt gtgtcatagt ttaaaagaaa gtcttaacgc
     1621 ttacttactg taggttgagt ttatttttga taataaaatt agtggcgagg taaaacatta
     1681 tcatttcaat aaattgttcc aaaataagaa gataaattgt tttttgctca gtaaatggga
     1741 aactaccggc acttgtatta atattaaaga tgtgactgtg aacatatgtt gcagcaacgc
     1801 taatcagaat aactaatata aaatatgcaa taaaggccat caagcctcgc cgattggaaa
     1861 aaagttgacc tatagaaatg gaaagataaa ttaataagat actcgtaaat gttgataata
     1921 ggacataggc tagtacagtt agcatattga tgaaataatc caccttaaaa ctattaaaga
     1981 atccttctaa tagttcgttg atgttaaaca ttggtacaat tacaatagcg atagcaaatg
     2041 caaggatgat agtattgaaa acactgcaaa taaaagaagc tagtagtttt gataaaataa
     2101 tttgatgcga attgacgggt aaagtcaacg ttaagtatcc ttcccgtcca aaaacacttt
     2161 tgctaaaacg tttgataatg ataagtaatg ttgataagag ggaaccagcg atcagggagc
     2221 caaaagttaa acctaatgtc agaggcaaca tcttattggt tagtactcca aatagtccat
     2281 cactattact ttgagcaaac agttttatcg taaatgacaa aatggcagcg atggcaataa
     2341 cgaaagcatt aagcgcaaag taccattttc caatagatct aaattcgtat tttaataatt
     2401 ttccaaacat gatatcctcc taaaccttat aagtttccct aaagagggaa tcaatggatt
     2461 gctggtattc ttgtcttaga tcatcagcat tgccagataa gaaaagtctt ccttgtttca
     2521 agaaaatgac ttcgtcaagg ataggttcaa tgtccgaaat caagtgggtt gagataataa
     2581 cagaagcatt ttcacaataa ctattaatga tggtttttag gatgtagtct cttgcagcag
     2641 ggtcaactcc accaataggt tcatcaagta catagagtct ggctttacga ctcatcacaa
     2701 gaattaattg tactttttct ttgtttcctt tggatagagt tttgaagcgg tcttcagggt
     2761 ctagttctaa atctcttaga aggcttgttg ctttggattt gtcaaagtcg ctgtaaaaat
     2821 cactaaagaa ctccaacatg tccttaattc tcatattttc attgagataa gtagtatctg
     2881 gtaaataaga aataattttt tttgtctcaa cacagggacg gtagccatca atgacaattt
     2941 ctcctttatt tggttgtaaa aggccattaa tgagtttaat taaagttgtt ttaccacttc
     3001 catttggacc taataatccg ataattttac cgttcgggat agtaatggtt aagtcatcaa
     3061 tagctttttt ttcacggtaa gattttgaaa catgatgtag ttgcaatagg tgtgccatta
     3121 ttgtgaatcc tcctttaaaa agctcgtcaa aacagggata atctcagtgt gactaaatcc
     3181 catttttgtc atattggtga taaatgattc taattcacta attgctaact ctcgtctttt
     3241 acgagcaatt aatttttgat cgtcagttac aaaacggcca gcagtacgtt gcgaataaac
     3301 cattccttcc cgttcaagtt ctgtaaacgc tcgttgcatg gtgttaggat taacacctgc
     3361 gatttctgca tattcacgaa cagttggaag ttgatcacca ctcttgattt cttggctaat
     3421 aatttggatc atgacatgtt gtgctatctg cgcataaata ggagattttt catcaaattt
     3481 ccaagacatg tggtctcctt tctgagtaat atagatattt gtactaatta aatggtacag
     3541 aaaaaaaagg ggaatgtcaa gttttatgcc atttttcact ctttgagaag tagacgtaga
     3601 ttagagagaa aattgaagca agaaaacctt tttcatagta taatagggaa caagataacc
     3661 aaaggaggaa cttatgtttg ctcaacttga tactaaaact gtatactcat ttatggatag
     3721 tttaattgac ttaaatcatt attttgaacg agcaaagcaa tttggttacc acaccatagg
     3781 aatcatggat aaggataatc tttatggtgc ttaccatttt attaaaggtt gtcaaaaaaa
     3841 tggactgcag ccagttttag gtttggaagt agagattctc tatcaagagc ggcaggtgct
     3901 ccttaactta atcgcccaga atacacaagg ctatcatcag cttttaaaaa tttccacggc
     3961 aaaaatgtct ggcaagcttc atatggatta cctctgccaa catttggaag ggatagcggt
     4021 tattattcct agtaagggtt ggagcgatac attagtggtc ccttttgact actatatcgg
     4081 tgttgatcag tatactgatt tatctcatat ggattctaag aggcagctta tacccctaag
     4141 gacagttcgt tattttgcgc aagatgatat ggaaaccctg cacatgttgc atgccattcg
     4201 agataacctc agtctggcag agacccctgt ggtagaaagt gatcaagagt tagcagattg
     4261 tcaacaacta accgccttct atcaaacaca ctgccctcaa gctctacaga atttagaaga
     4321 cttagtgtca ggaatctatt atgatttcga tacaaattta aaattgcctc attttaatag
     4381 agataagtct gccaagcaag aattgcaaga cttgactgag gctggtttga aggaaaaagg
     4441 attgtggaaa gagccttatc aatcacgctt actacatgaa ttggtcatta tttctgacat
     4501 gggctttgat gattattttt tgattgtgtg ggatttactt cgctttggac gcagtaaagg
     4561 ctattatatg ggtatgggac gtggctcggc ggcaggtagt ctagtggctt atgctctgaa
     4621 cattacaggg attgatccag ttcaacatga tttgctattt gagcgctttt taaacaaaga
     4681 acgttatagc atgcctgata ttgatatcga tcttccagat atttaccgtt cagaatttct
     4741 acggtatgtc cgaaatcgtt atggtagcga ccattcggcg caaattgtga ccttttcaac
     4801 ctttggccct aaacaggcta ttcgtgatgt tttcaaacgg ttcggggttc cagaatacga
     4861 actgaccaat ctcactaaaa aaattggttt taaagatagc ttggctactg tctatgaaaa
     4921 gtcaatctct ttcaggcagg ttattaatag tagaactgaa tttcaaaagg cctttgctat
     4981 tgccaagcgt atcgaaggaa atccaagaca aacgtccatt cacgcagctg gtattgtgat
     5041 gagtgatgat accttgacca atcatattcc tctaaaatcg ggcgatgaca tgatgatcac
     5101 ccagtatgat gctcatgcgg tcgaagctaa cggcctgtta aaaatggatt ttttggggtt
     5161 aagaaatttg acctttgttc aaaaaatgca agagaaggtt gctaaagact acgggtgtca
     5221 gattgatatt gcagccattg atttagaaga cccgcaaacg ttggcacttt ttgctaaagg
     5281 ggataccaag ggaattttcc aatttgaaca aaatggtgct attaatcttt taaaacggat
     5341 taagccacaa cgttttgaag aaattgttgc cactaccagt ctaaatagac caggggcaag
     5401 tgactatacc actaatttca ttaaacgaag agaaggacaa gaaaaaattg atttgattga
     5461 tcctgtgatt gcttccattt tagagccaac ttacggtatt atgctttatc aagaacaagt
     5521 tatgcagatt gcacaggttt atgctggttt tacgttaggc aaggccgact tgttaaggcg
     5581 tgccatgtct aaaaaaaatc tacaagaaat gcaaaaaatg gaagaagact ttattgcttc
     5641 tgctaagcac ctagggagag ctgaagaaac agctagagga ctttttaaac ggatggaaaa
     5701 atttgcaggt tatggtttta accgcagcca tgcctttgcc tattcagctt tagcttttca
     5761 attggcttat ttcaaagccc attacccggc tgttttttac gatatcatga tgaattattc
     5821 tagcagtgac tatatcacag atgctctaga atcagatttt caagtagcgc aagttaccat
     5881 taatagtatt ccttacactg ataaaattga agctagcaag atttacatgg ggctgaaaaa
     5941 tattaagggg ttgccaaggg attttgctta ttggattatc gagcaaagac catttaatag
     6001 cgtagaggat tttctcacta gaactccaga aaaatatcaa aaaaaggttt tccttgagcc
     6061 tctgataaaa ataggtctgt ttgattgctt tgagcctaac cgtaaaaaaa ttctggacaa
     6121 tttggatggt ttactggtat ttgttaatga gcttggttct cttttttcag attcttcctt
     6181 tagttgggta gatacgaaag attactcagc aactgaaaaa tattctttgg aacaggagat
     6241 cgttggggtt ggcatgagca agcatccttt aattgatatt gctgagaaaa gtacccaaac
     6301 ttttactccc atttcacagt tagtcaaaga aagcgaagca gtcgtactga ttcaaataga
     6361 tagcattagg atcattagaa ccaaaacaag tgggcagcaa atggcttttt taagtgtgaa
     6421 tgacactaag aaaaagctcg atgtcacact ttttccacaa gagtatgcca tttataaaga
     6481 ccaattaaaa gaaggagaat tctattactt aaaaggtaga ataaaagaaa gagaccatcg
     6541 actgcagatg gtgtgtcagc aagtgcaaat ggctattagt caaaaatatt ggttattagt
     6601 tgaaaaccat cagtttgatt cccaaatttc tgagatttta ggtgcctttc caggaacgac
     6661 tccagttgtt attcactatc aaaaaaataa ggaaacaatt gcattaacta agattcaggt
     6721 tcatgtaaca gagaatttaa aggaaaaact tcgtcctttt gttctgaaaa cggtttttcg
     6781 ataaaaatgg acaattagtg aagtattttg caaatgaatg tgctataata agaccgttaa
     6841 attaattaaa ggagtaccag aaaatgaaac gtattgctgt tttaaccagt ggcggcgacg
     6901 ctcctggtat gaacgctgct attcgtgcag tcgttcgtaa agcaatttca gaaggaatgg
     6961 aagtttatgg tatcaaccgt ggctatgcag gtatggttga tggcgacatt ttcccactgg
     7021 gttcaaaaga agttggtgat aaaatctcac gtggtggaac tttcttatac tcagctcgtt
     7081 atccagagtt tgctcaactt gaaggtcaat tggctggtat tgagcaactt aaaaaacatg
     7141 gtattgaagg tgttgttgtt attggtgggg atggttctta tcatggcgct atgcgcttga
     7201 cagagcacgg cttcccagcg gtaggtatcc caggaacaat cgataacgat attgcgggaa
     7261 ctgactacac tatcggattt gatacagcag ttaacacagc agttgaagct attgataaac
     7321 ttcgtgacac ctcatcaagt cacggacgta cttttgtggt tgaagtgatg ggtcgcaatg
     7381 cgggtgatat tgctctttgg gcaggtattg ctagtggtgc tgatcaaatc attgttccag
     7441 aagaagagtt tgatattgaa aaagtggcat caacaatcca atatgatttt gaacacaaag
     7501 gtaaaaatca ccacattatc gtcttagctg aaggtgttat gagcggtgaa gcttttgctc
     7561 aaaaactgaa agaagctggc gataagagtg accttcgtgt gacaaatctt ggccacattc
     7621 ttcgtggggg ttcaccaact gctcgtgacc gtgtcattgc ttcttggatg ggttcacatg
     7681 ctgttgagct acttaaagaa ggcaaaggtg gtttggctgt tggtattcac aatgaagaac
     7741 ttgttgaaag cccaatcctt ggtactgcag aagaaggtgc tttgttcagc ttgactgaag
     7801 aaggcaagat tattgttaat aatccacaca aagctcgttt agactttgca gctttgaacc
     7861 gttcattgtc acaataagtt tagttaaaat tgttaaatcg tcaattattg acaaaaaaat
     7921 aagggagttt cagagtaaaa tgaataaacg cgtaaaaatc gttgcaacac ttggtcctgc
     7981 ggttgaaatc cgtggtggta aaaaatacgg agaagatggc tactgggctg gtcaacttga
     8041 tgttgaagaa tctgcaaaaa aaatcgctga attaattgaa gctggtgcga atgttttccg
     8101 tttcaatttc tcacatggtg accacaaaga acaaggtgac cgtatggcaa ctgttcgcct
     8161 tgcagaagaa attgctcgtc aaaaagttgg tttccttttg gatactaaag gtcctgaaat
     8221 gcgtacggaa ttgtttgcag atgatgctaa agagtttagc tatgttactg gagaaaaaat
     8281 ccgtgtagcg actacacaag gtattcaatc aactcgcgat gttattgctt tgaacgttgc
     8341 tggtagcctt gacatctacg atgaagtaga agtaggtcat actattctta tcgatgatgg
     8401 taaacttggt ttgaaagtga tcgacaaaga tatcgcaaca cgtcaattta tcgttgaagt
     8461 agaaaacgac ggtattattg ctaaacaaaa aggtgttaat atccctaaca ctaaaattcc
     8521 tttcccagca cttgcggaac gcgacaatgc tgatatccgt tttggtcttg aacaaggact
     8581 taactttatt gcaatctcat ttgttcgtac agcaaaagac gttgaggaag ttcgtgaaat
     8641 ctgtcgcgaa actggtaacg accatgtgca attatttgct aagattgaaa accaacaagg
     8701 tatcgataat cttgatgaaa tcatcgaagc tgctgatggt atcatgattg cacgtggcga
     8761 catgggtatc gaagtaccat ttgaaatggt tccagttttc caaaaaatga ttatcactaa
     8821 agtgaatgca gctggtaaag cagttatcac agcaacaaac atgcttgaaa caatgactga
     8881 aaaaccacgt gcgacacgtt ctgaagtatc tgacgtcttc aatgctgtta tcgatggtac
     8941 tgatgcaaca atgctttcag gagagtccgc taacggtaaa tacccggttg aatctgttcg
     9001 tacaatggcg actattgatc gtaatgcaca aactcttctt aacgaatatg gtcgtttaga
     9061 ttcatctgcc tttccacgta ccaataaaac agatgttatt gcttcagctg ttaaagatgc
     9121 cactcattca atggacatca aacttgttgt gactattact gagacaggta atacagctcg
     9181 tgcgatttct aaattccgtc cagatgcgga catcttagct gtaacatttg atgaaaaagt
     9241 acaacgtgct ttgatgatta actggggtgt tattcctgtt cttgctgaaa aacctgcatc
     9301 tactgatgac atgtttgaag tagcagagcg tgttgctgtg gaggcaggct tagttcaatc
     9361 tggggataat attgttattg ttgctggtgt tccagttggt actggtggaa cgaatactat
     9421 gcgtgttcgt acagtaaaat aaatgtgtta taactattta cctaaaggtt agaaggcaag
     9481 cttctaacct tttttgatat caaaaatatc atgttaattc actagtgtta at
//
LOCUS       DAUDYX010000061         9370 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.062, whole genome shotgun sequence.
ACCESSION   DAUDYX010000061 DAUDYX010000000
VERSION     DAUDYX010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9370
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.062"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(162..1475)
                     /locus_tag="VHD02_001647"
     CDS             complement(162..1475)
                     /locus_tag="VHD02_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEQ5498238.1"
                     /translation="MTIIIMDSNSAHETDNLSVSFLNFCYNSLMKRHFLLLTFYLFLT
                     GLTAGLVAFILTKAIHLIQSLSFGFSQGSFSTMIASVPPQRRALSLLFAGLLAGLGWH
                     LLAKKGKDIQSIQQIIQDDISFSPWTQFWHGWLQLTTVSMGAPVGREGASREVAVTLT
                     SLWSQRCNLSKADQKLLLACASSAALGAVYNAPLATILFILEAILNRWSLKNIYAACL
                     TSYVAVETVALLQGRHEIQYLMPQQHWTLGTLIWPVLAGLILSLFAHAYKHLLEHLPK
                     ADAKSQWFIPKVLIAFSLIAGLSIFFPEILGNGKAGLLFFLHEEPHLSYISWLLVAKA
                     VAISLVFASGAKGGKIAPSMMLGGASGLLLAILSQYLIPLSLSNTLAIMVGATIFLGV
                     INKIPLAAPVFLVEITGQSLLMIIPLALANLIFYFSYQFYRFILK"
     gene            complement(1450..2409)
                     /gene="nrdF"
                     /locus_tag="VHD02_001648"
     CDS             complement(1450..2409)
                     /gene="nrdF"
                     /locus_tag="VHD02_001648"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEQ5498239.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSLQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKKEIEEIFEWTNNNEFLQEKARIINDIYANGDALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMSDDDYNYGL"
     gene            complement(2731..4890)
                     /gene="nrdE"
                     /locus_tag="VHD02_001649"
     CDS             complement(2731..4890)
                     /gene="nrdE"
                     /locus_tag="VHD02_001649"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEQ5498240.1"
                     /translation="MSLKDLGDISYFRLNNEINRPVNGKIPLHKDKEALKAFFAENVL
                     PNTMSFTSITEKIEYLISNDYIESAFIQKYHPEFITELAGIIKSENFRFKSFMAAYKF
                     YQQYALKTNDGEHYLENLEDRVLFNALYFADGQEDLAKDLAVEMINQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARKNEDMYLFSPYNVEKEYGIPFNYLDITNMYDELVANPKI
                     TKTKIKARDLETEISKLQQESGYPYIINIDTANKANPINGKIIMSNLCSEILQVQTPS
                     LINDAQEFVEMGTDISCNLGSTNILNMMTSPDFGRSIKTMTRALTFVTDSSSIEAVPT
                     IKHGNSQAHTFGLGAMGLHSYLAQHHIEYGSPESIEFTDIYFMLLNYWTLVESNNIAR
                     ERQTTFVGFENSKYANGSYFDKYVTGHFVPKSDLVKSLFKDHFIPQAEDWKALRDAVQ
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLSTDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFLRSELPMELYEWKTQSKQTTRDLS
                     ILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI"
     gene            complement(4910..5128)
                     /locus_tag="VHD02_001650"
     CDS             complement(4910..5128)
                     /locus_tag="VHD02_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEQ5498241.1"
                     /translation="MITVYSKNNCMQCKMTKKFLEQHGANFQEINIDEHPEKVDYVKS
                     LGFTSAPVIEADNLVFSGFQPAKLKELI"
     gene            5521..5784
                     /locus_tag="VHD02_001651"
     CDS             5521..5784
                     /locus_tag="VHD02_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEQ5498242.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADAEDAIAAIEETMTKEGLA"
     gene            5789..7522
                     /gene="ptsP"
                     /locus_tag="VHD02_001652"
     CDS             5789..7522
                     /gene="ptsP"
                     /locus_tag="VHD02_001652"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEQ5498243.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVADTNAEEARL
                     DVALQAAQDELSVIRENAVESLGEEAAAVFDAHLMVLADPEMISQVKETIRAKQTNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGD
                     VIAVNGITGEVIIDPSEDQVLAFKEAGAAYAKQKAEWSLLKDAHTETADGKHFELAAN
                     IGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQMRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAIFDEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDSAKMEEYANRALTECSTAEEV
                     LELSKEYVSED"
     gene            7707..9134
                     /locus_tag="VHD02_001653"
     CDS             7707..9134
                     /locus_tag="VHD02_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019322885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEQ5498244.1"
                     /translation="MAKQYKNLVNGEWKLSENEITIYAPATGEELGSVPAMTQAEVDA
                     VYASAKKALSDWRALSYVERAAYLHKAADILVRDAEKIGAILSKEVAKGHKAAVSEVI
                     RTAEIINYAAEEGLRMEGEVLEGGSFEAASKKKIAIVRREPVGLVLAISPFNYPVNLA
                     GSKIAPALIAGNVVALKPPTQGSISGLLLAEAFAEAGIPAGVFNTITGRGSVIGDYIV
                     EHEAVNFINFTGSTPIGEGIGKLAGMRPIMLELGGKDSAIVLEDADLALAAKNIVAGA
                     FGYSGQRCTAVKRVLVMDKVADQLAAEIKTLVEKLSVGMPEDDADITPLIDISAADFV
                     EGLIKDATDKGATALTAFNREGNLISPVLFDHVTTDMRLAWEEPFGPVLPIIRVTTVE
                     EAVKISNESEYGLQASIFTTNFPKAFGIAEQLEVGTVHLNNKTQRGTDNFPFLGAKKS
                     GAGVQGVKYSIEAMTTVKSVVFDIQ"
     gene            9229..>9370
                     /locus_tag="VHD02_001654"
     CDS             9229..>9370
                     /locus_tag="VHD02_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deacetylase"
                     /protein_id="HEQ5498245.1"
                     /translation="MKKLNVILVGLLSILMLSLAIVFINRWKLNEDSQRIVLAEKKKN
                     TSD"
BASE COUNT         2975 a         1660 c         1967 g         2768 t
ORIGIN      
        1 ctcttcctca aggatgagtt ttacagcaga gagtttgaag gctttatcaa aggtttttct
       61 tggcataatg actattcctc gttttcactt aattttgtgt ctactttagt ataccaagtc
      121 caattcatcc gaagcttttt attaaaaaag taattaacta atcatttaag gatgaaacga
      181 taaaactgat agctaaaata aaaaatgagg ttagctaatg ctaaagggat aatcattagt
      241 aacgattggc ctgtaatttc aaccagaaaa actggtgccg ccaagggtat cttattgatc
      301 acacctaaaa agattgttgc accaaccata atggctaacg tatttgatag agacaaggga
      361 ataaggtact gactaaggat agctagtagt aagccactag cacctccaag catcatagaa
      421 ggagcaattt tacccccttt tgctccagaa gcaaaaacta aagagatagc gacagctttg
      481 gcaacaagta gccagctaat atagctcaga tgtggttctt catggagaaa aaagagtaat
      541 ccagctttgc catttcctaa aatctctggg aagaaaatgc tgagtcctgc gataaggcta
      601 aaagcgatga gaactttcgg aataaaccac tgacttttgg catcagcctt aggaaggtgt
      661 tccaaaagat gtttgtaagc gtgagcaaag agggaaagga taagaccagc caggacgggc
      721 caaataaggg ttcctagcgt ccaatgttgc tgaggcatta ggtattgaat ctcatgtcgg
      781 ccttgtaata aagcaactgt ttccacagcc acatagctcg ttaggcaggc agcgtatata
      841 tttttaaggg accaacggtt aagaatagct tccaagataa ataaaatagt ggctaaggga
      901 gcattgtaga cagcaccaag ggcagcgcta gaagcgcaag ctaataaaag tttttgatcg
      961 gcttttgaca agttgcatct ttggctccaa agagacgtca aggtaacagc gacttcccgt
     1021 gaagctcctt ctcggccgac aggagctccc atagatacgg tcgttaattg gagccaacca
     1081 tgccaaaact gtgtccaggg actaaatgag atgtcatctt gaatgatttg ctgaatagat
     1141 tgaatgtcct ttcctttttt agccaatagg tgccacccaa gacctgcaag aagtccagca
     1201 aagagaagtg aaagagctcg tctttgtggt ggaacgctag caatcatagt gctaaaagaa
     1261 ccttgactga atccaaaact gagggactga atcaggtgaa tggcttttgt taagataaaa
     1321 gcaacaaggc cagctgttaa acctgttaaa aagaggtaaa aggtcaagag taaaaagtgt
     1381 cgtttcataa gactattata acaaaagtta agaaaagaga ctgatagatt atcagtctcg
     1441 tgagcactat tagagtccat aattataatc gtcatcagac atagcttcta cagaaccaag
     1501 aagatagcca ttccctacct gtgagaaaaa gtcatggttt gatgttcctg ttgaaatacc
     1561 attcataaca attgggttaa catcgtttgc agtatctggg aataaaggat cttgtcctaa
     1621 attcataaga gctttgttag cattgtagcg taaaaaggtc ataacttcct cagtccaccc
     1681 tacgccatca taaagcgttt tggtgtattt ttcttcgttt tcatacagct gataaaggag
     1741 gtcatacatc caatcacgaa aattctcttg ctcatcttct ggtagttcgt taaaaccaag
     1801 ctggaatttg taaccgatat aagtaccatg tacagattca tcacgaataa ttagtttaat
     1861 gatttcagca acatttgcta gcttattatt tcccaaatag taaagagggg tgaaaaagcc
     1921 agaataaaaa aggaaagttt cgagatatgt tgaggcaact ttcttttgta aggcatctcc
     1981 attagcgtaa atgtcattga taatacgtgc tttttcttga agaaactcat tattattagt
     2041 ccactcaaag atctcttcaa tttctttttt ggtgtttaag gttgaaaaga tagacgaata
     2101 agatttagcg tgaacagatt ccatgaattg aatattgttt aagacagctt cttcgtgagg
     2161 agtgcgaaca tcagcacgaa tagcttcgac accagtttct gattgcatgg tatcaagtag
     2221 ggttaatcct ccaaaaacct tgccaacaag atctttttct tgaagagaaa gtttgcgcca
     2281 atcgtctaag tcatttgata aagggatacg ggtatcaagc caaaattgtt cggtcaactt
     2341 ttcccaagtt gacttgtcaa tgacgtcttc gatttcattc cagttaattg cttcataata
     2401 tgtagtcata gaaaaatgat ctcctaaaga gtagtaaagt gcgttaagga tatcgagata
     2461 aaccagtaga accgaccaaa gttctagtgg taataataag tttaaataga ttcaaaaggg
     2521 taacgaatga gttagtgagt ggtttaggca gatcgcctaa agcgactgcc gtagaagtaa
     2581 tagatgcttt agcatcatta gggcatatcg aggaattacg acacggagta aggaaagtcg
     2641 ataatacttt ccttacatag ttagtgagtg gtttaggcag atcgcctaaa gcgactgccg
     2701 tagaatttag catcattact tcttaaccaa ttaaatgaca caagattcac attggtttgc
     2761 gcccacttct tccccatcat ccgtaaaggt acggatatag tagatagatt taatgccttt
     2821 attgaaagcg tagtttcgta agatggataa atcacgagtg gtttgtttgc tttgtgtttt
     2881 ccactcataa agctccatag gcaactcact acgaaggaat agagttaatg acaagccttg
     2941 gtccacatgt tcggtcgcag cggcatagac atcaataact ttgcgcatgt ccatatcgta
     3001 agcagatgta tagtaaggaa tggtatccgt agacaaacca tttgcaggat agtagatttt
     3061 accaattttc ttttcttgac gctcttcgat gcgttgtgtg attgggtgaa tagaagcaga
     3121 gcagtcattg atataagaaa tagagccatt tggagcaact gctagacggt tttgatgata
     3181 aagaccatct ttttgaacgg cgtcgcgaag agctttccaa tcctctgctt gaggaataaa
     3241 gtgatcttta aagagacttt tgaccaaatc agattttgga acaaagtgtc ctgtaacgta
     3301 tttatcaaag taactaccat tagcgtactt agagttctca aagccaacaa aggtagtttg
     3361 gcgctcacga gcgatgttat tggattcgac caaggtccaa taattcagga gcataaagta
     3421 aatatcagta aactcgatgg attctggact gccatattca atatgatgtt gagcaaggta
     3481 agaatgtagt cccatagctc caaggccaaa agtatgagct tggctattgc catgtttaat
     3541 ggttggaaca gcttcaatgc ttgatgaatc agtaacaaaa gttagggcac gtgtcatggt
     3601 cttaatagaa cggccaaagt ctggtgaggt catcatgttc aggatattag tggaacctaa
     3661 gttacatgaa atatcagttc ccatttctac aaactcttgc gcatcattga taaggctagg
     3721 tgtttgaact tgtaaaattt cagaacacaa gttgctcatg atgatttttc cattgatagg
     3781 attagcttta ttagctgtat caatattgat gatataaggg taaccagatt cttgttgtaa
     3841 ttttgaaatc tctgtttcaa gatcacgagc tttaatttta gtcttagtaa ttttagggtt
     3901 tgccactaac tcatcgtaca tattggtaat gtcgagatag ttaaagggaa tgccatattc
     3961 tttttcaaca ttgtaaggac taaagagata catgtcctcg tttttacgag ctaattcgta
     4021 gaatttatca ggaacggtaa tccctagtga caaggtttta acacgtacct tttcatcggc
     4081 attttctttt ttagtagata agaaagcaat gatatcagga tgaaaaacat ttaggtaaac
     4141 aacaccagct ccttgacgtt gcccaagttg atttgaataa gaaaaactat cttcaaataa
     4201 tttcataaca ggaacaactc ctgaggctgc accagcatag cctttgattg gtgcgccagc
     4261 ttcacggagg ttagacaagg taatcccaac acctccacca atacgggata attgcaaagc
     4321 agagttgata gaacgtccga tagagttcat gtcatcagtt acttgaatca agaaacaaga
     4381 gaccaattca ccacgacggc ttcgaccagc atttaaaaag gaaggagtag ccggttggta
     4441 acgttggtta atcatttcaa cggctaaatc ttttgctaag tcttcttgac catctgcaaa
     4501 atacaaagca ttaaacaaga cgcggtcttc aaggttttct aaataatgct ctccatcatt
     4561 tgtttttaag gcgtattgct ggtagaactt gtaggctgcc ataaatgatt taaagcgaaa
     4621 attttctgat ttgattatgc cagctaattc agtaataaat tcagggtggt atttctgaat
     4681 aaaagctgat tcaatgtaat catttgagat taaatactca attttttccg taatggaagt
     4741 aaaagacatg gtgtttggca gcacattttc agcgaaaaaa gcttttaaag cttctttgtc
     4801 tttatgaagt ggaattttac cattaacagg acggttaatt tcattattta ggcgaaaata
     4861 tgaaatatcg ccaagatctt tgagactcat attatttttc cttgtcttat taaatcaatt
     4921 cttttaactt tgcaggttgg aagccagaaa atacgaggtt atctgcttcg ataacaggag
     4981 cagatgtgaa gccaagactt ttcacataat ccactttttc aggatgttca tcgatattaa
     5041 tttcctggaa gttggctccg tgttgttcta aaaatttctt agtcattttg cactgcatgc
     5101 aattattttt ggaatatact gtaatcatgt cttgtccttc taaaattagt aatggttcca
     5161 tcttaaatgg aattactata atagcatata aaaaataaag ctaaaaagca agctataaac
     5221 ttatctgaaa cacaacatat tgtgttgaaa taaaaaacag aaacctaaat attgtgtcaa
     5281 tctggattct tatggttaga gctctagcgt tttggtgata gaagctaagc agttttaata
     5341 aagcattaaa gtctgaaaaa taaattgtga aaaacgtgaa aataaaagaa aataatttca
     5401 cattttttgt cgtttaaata tttaatcttg aaaataaaaa agcattgtgc tataatcggt
     5461 tgttgtaagg gttatcatgt gatggctcaa aaatacacaa cttaaaagga gaaccatact
     5521 atggcttcaa aagactttca cattgttgca gaaacaggta ttcatgcgcg tccagcgact
     5581 ttgcttgttc aaacagctag caaatttgct tcagacatca ctcttgacta caaaggtaaa
     5641 gcagtaaatc taaaatcaat catgggtgtt atgagtcttg gtgttggtca aggtgcagat
     5701 gttactatct cagctgaagg tgcagatgct gaagatgcca ttgcagcaat tgaagaaaca
     5761 atgactaaag agggattggc ataagactat gacagaaatg cttaaaggaa ttgcagcctc
     5821 agacggcgtt gctgttgcta aagcatatct actagttcag ccggatttgt catttgagac
     5881 tgttacagtc gcagatacaa atgcagaaga agctcgcctt gatgttgcac tccaagctgc
     5941 acaagacgag ctttctgtta tccgtgaaaa tgcagtagaa agcttaggtg aagaagcagc
     6001 agccgttttt gatgcccatt taatggttct tgctgatcca gaaatgatca gccaggttaa
     6061 agaaacgatt cgcgcaaaac aaacgaatgc agaaacaggt cttaaagaag tgactgacat
     6121 gttcatcacc atctttgaag gcatggaaga taacccatac atgcaagaac gtgcagcgga
     6181 catccgcgac gttgcaaagc gtgtgttggc tcacctttta ggtgtaaaac ttccaaatcc
     6241 agctacaatc aatgaagaat caatcgttat cgcacacgat ttgacacctt cagatactgc
     6301 tcaacttaac aaacaatttg taaaagcatt tgttacaaat atcggtggtc gtacaagtca
     6361 ctcagctatc atggcacgta cacttgagat cgctgcggta cttggaacaa atgatattac
     6421 aaaacgtgtt aaagatggtg atgtgattgc cgttaatggt atcactggtg aagtgattat
     6481 cgatccaagc gaagatcaag tacttgcttt taaagaagct ggtgcggctt atgccaaaca
     6541 aaaagcagag tggtctctcc ttaaagatgc gcatactgaa acagctgatg gcaaacactt
     6601 tgaattggct gctaatatcg gtacgcctaa agacgttgaa ggtgttaatg acaatggcgc
     6661 tgaagctgtt ggcctttacc gtactgagtt cttgtacatg gattctcaag acttcccaac
     6721 tgaagacgaa caatacgaag cttacaaggc agtgcttgaa ggcatgaatg gcaaacctgt
     6781 tgtggttcgt acgatggata ttggtggcga caaggaactt ccttactttg accttccaaa
     6841 agaaatgaat ccattccttg gtttccgtgc tcttcgtatt tccatctctg aaactgggga
     6901 tgccatgttc cgcacacaaa tgcgtgcgct tcttcgtgcc tctgttcacg gacaacttcg
     6961 tattatgttc ccaatggttg cccttcttaa agaattccgt gctgcaaaag caatctttga
     7021 tgaagaaaaa gcaaacttgc ttgcagaagg cgttgcggtt gctgatgaca tccaagttgg
     7081 tatcatgatt gagattcctg cagctgctat gcttgcagac caatttgcta aggaagttga
     7141 tttcttctca attggaacaa acgaccttat ccaatacact atggcagcag atcgtatgaa
     7201 cgaacaagta tcataccttt accaaccata caacccatca atattacgtt tgatcaacaa
     7261 tgtgatcaaa gcagcgcacg ctgaaggtaa atgggcaggt atgtgtggtg agatggcagg
     7321 tgaccaacaa gctgttccac ttcttgtcgg aatgggcttg gatgagttct ctatgtcagc
     7381 aacttcagta cttcgtacgc gtagtttaat gaagaaactt gactctgcta agatggaaga
     7441 atatgcaaat cgtgcgctta cagaatgttc aacagcagaa gaagttcttg aactttctaa
     7501 agaatacgtt tctgaagatt aacgggttaa agagttggtt taaaccagct cttttttgct
     7561 agctaaaaat acaattactt tttggtgtga aatcatttga gttatcaaat aattatcaga
     7621 attttcagat atttagtgta gccaaatctt gaattatatc ggaaaagtga tagaataaga
     7681 ttactatcat aaaaggagat aaaacgttgg caaaacaata taaaaattta gtgaacggtg
     7741 aatggaaact atcagaaaac gagattacca tttacgcacc agcaacaggt gaagagttag
     7801 gatcagttcc agcgatgacg caggcagagg tagatgctgt ttacgcttca gctaaaaagg
     7861 ctctatcaga ttggcgcgcc ttgtcttatg tggaacgtgc agcttacctt cataaagcgg
     7921 ctgatatttt agtacgtgat gctgaaaaga tcggcgcgat tctttcaaaa gaagtagcca
     7981 aaggtcacaa ggcagctgtc agtgaagtta ttcgtaccgc tgaaatcatt aattatgcag
     8041 cagaagaagg gcttcgtatg gaaggtgaag ttcttgaagg tggtagcttc gaagccgcaa
     8101 gtaagaagaa gattgctatt gttcgtcgtg aaccagttgg tttagttctt gccatctcac
     8161 cttttaatta tcccgttaac ttggcaggtt ctaaaattgc tccagccctt attgcaggaa
     8221 atgttgttgc tcttaaacca ccaacacaag gctctatttc tggtttgtta ctagcagaag
     8281 cttttgcaga agctggtatt ccagcaggtg tctttaatac cattacaggg cgaggttctg
     8341 ttattgggga ttatatcgtt gagcacgaag cggttaactt tatcaacttt acaggttcta
     8401 ctccaattgg ggaaggaatt ggtaaattag cgggtatgcg accaattatg cttgagcttg
     8461 gtggtaagga ttctgctatc gttttggaag atgcagatct tgctttagca gcgaaaaata
     8521 ttgtagccgg tgcttttggt tactcaggac aacgttgtac agcggttaaa cgcgttcttg
     8581 tgatggacaa ggtggcggat caattggcgg ctgagattaa aacccttgtt gaaaaactaa
     8641 gtgtcggaat gcctgaagac gatgctgata ttacaccatt aattgatata tcagctgctg
     8701 attttgttga agggttgatt aaagatgcaa ctgataaggg agctactgct ttgacagcct
     8761 ttaatcgtga aggcaatctt atttcacccg ttctctttga tcatgtgaca actgacatgc
     8821 gtttggcgtg ggaagagccg ttcggcccag tattaccaat tattcgtgta accactgtag
     8881 aagaagccgt caagatttct aatgagtctg aatatggttt gcaagcttct atttttacaa
     8941 ctaatttccc aaaagctttt ggcattgctg agcaattaga agttggaact gttcacctta
     9001 acaataaaac acaacgtgga acagataatt tcccattctt aggcgctaaa aaatcaggtg
     9061 caggggtaca aggagttaaa tattctatcg aagctatgac aactgttaaa tctgttgtat
     9121 ttgatatcca gtaataaaaa aaataaacaa aaagttaggt taacttaact ttttgtttat
     9181 ttttttaatg ttttttgtta caatagtcta tacagatgga ggcaactatt gaaaaaatta
     9241 aatgttattc ttgttggttt attaagcatt ctgatgttga gtttagctat tgtgtttatt
     9301 aatcgttgga aactaaacga agatagtcag cgtatagttt tggctgaaaa gaaaaaaaac
     9361 acgtcagatt
//
LOCUS       DAUDYX010000062         8673 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.063, whole genome shotgun sequence.
ACCESSION   DAUDYX010000062 DAUDYX010000000
VERSION     DAUDYX010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.063"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(<1..133)
                     /locus_tag="VHD02_001655"
     CDS             complement(<1..133)
                     /locus_tag="VHD02_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEQ5498246.1"
                     /translation="MIDFIISIDDCAVELDSRQSWKIRYPLSTILFLVFVCQLAGIET
                     "
     gene            688..2196
                     /locus_tag="VHD02_001656"
     CDS             688..2196
                     /locus_tag="VHD02_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ5498247.1"
                     /translation="MLDYYLESDIIDQKILLAILLTEKELTMAAACSQTTLTALKIKQ
                     YLRQFNALFKGYLRIELIKSRIYCEVLNDKREAFFYDIFALSDTLKMLTFLLLDNPKH
                     KSIAVYTRKQGISQSKAYRLIHKLKHYLQDIGLNIVDNTVIGDELKIRYLIALLHKEY
                     GIILYDIQPADIETIHAFIFAAQKNLQPSAFLDRRFLFFDVLLMLTWKRHRYPVHLPH
                     LALFEHLKSLPIFDNIKTIAVDELAPRTRVTFSFDDFDYLFLIYLTTDNSFLSGYWTS
                     HQRQQLYHLITKDPDYHLLIHRLQALVGKYSDIHEHIPNLIPFFKRTLYNLQTLITFD
                     GYYFDQYQGNMLLLDKLETVIKDWLYDTGRQGSISSGHLHLMCLYLEQILESSIAPIN
                     ITVIESQETVGNVIANFITSTIPSYKVELSRVNILSDNIYPYDKPVDLVVTSQKLLPF
                     LKELGVFPKETRLFGLSLDCIQQQREDLIKTILALHQNHYQKRLEELWRIPS"
     gene            complement(2545..3894)
                     /locus_tag="VHD02_001657"
     CDS             complement(2545..3894)
                     /locus_tag="VHD02_001657"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEQ5498248.1"
                     /translation="MSHITFDYSKVLESFAGQHEIDFLQGQVTEADKLLREGTGPGSD
                     FLGWLDLPENYDKEEFARILTAAEKIKSDSEVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTAKERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVIRVDK
                     PRKNVIIPELAEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPAQDEF
                     TLGYTIYFFELAIAVSGYMNAVNPFDQPGVEAYKRNMFALLGKPGFEALSAELNARL"
     gene            complement(4353..5057)
                     /gene="speG"
                     /locus_tag="VHD02_001658"
     CDS             complement(4353..5057)
                     /gene="speG"
                     /locus_tag="VHD02_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeG"
                     /protein_id="HEQ5498249.1"
                     /translation="MKTNILTIIILSCVFSYGSQLAYADENLKDLKRSLRFAYNITPC
                     DYENVEIAFVTTNSIHINTKQKRSECILYVDSIVSLGITDQFIKGDKVDVFGLPYNFS
                     PPYVDNIYGGIVKHSNQGNKSLQFVGILNQDGKETYLPSEVVRIKKKQFTLQEFDFKI
                     RKFLMEKYNIYDSESRYTSGSLFLATKDSKHYEVDLFNKDDKLLSRDSFFKRYKDNKI
                     FNSEEISHFDIYLKTY"
     gene            complement(5159..5257)
                     /gene="ffs"
                     /locus_tag="VHD02_001659"
     ncRNA           complement(5159..5257)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VHD02_001659"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5457..6689
                     /locus_tag="VHD02_001660"
     CDS             5457..6689
                     /locus_tag="VHD02_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HEQ5498250.1"
                     /translation="MGVMMKQKIKILTVIGLMTVGMSACHNTSKPSNTDSVFSLTGKK
                     RQQIVKQVRQRYYFQQLSKTEQENYLTLYDSLAQFREIISLTPVSKKSLIKTIDAFVM
                     DNPEFYWITSADYRFEFSDQTVFVTFPIPEDAKNVYQDLQAIGNDIVANTPSKDRYEQ
                     VKYFYEVIIRDTDYNKKAFEAYQSGSQSQVASNQDIKSVFIDHLSVCNGYAQAFQFLC
                     QKAGIPVAYIRGTGTSQQPQQSFAHAWNAVQINNTYYGVDVTWGDPVFDNHLSHQKQG
                     TINYSFLCLPDYLMALSHQPSKDIAFNTKERFENVWTIPSCTDDSLLYSKRHQSYIST
                     FDNDAILASLENQLLNRQEPLSLQFAHQDDYQQMVTDLTTNQTGYHNLFNQYWNNYTG
                     FTYGLLPETLSISFASRN"
     gene            complement(7303..7818)
                     /locus_tag="VHD02_001661"
     CDS             complement(7303..7818)
                     /locus_tag="VHD02_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEQ5498251.1"
                     /translation="MPYSLEEQTYFMQEALKEAEKSLQKAEIPIGCVIVKDGEIIGRG
                     HNAREESNQAIMHAEMMAINEANAHEGNWRLLDTTLFVTIEPCVMCSGAIGLARIPHV
                     IYGASNQKFGGADSLYQILTDERLNHRVQVERGLLAADCANIMQTFFRQGRERKKIAK
                     HLIKEQSDPFD"
     gene            complement(7818..8597)
                     /locus_tag="VHD02_001662"
     CDS             complement(7818..8597)
                     /locus_tag="VHD02_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEQ5498252.1"
                     /translation="MKLTTLGSWGAYPYQDAGTTSYLVTGHDGFQLLMDAGSRALNEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLRHYFQLYPKHLWQPKVLPIYGHDEDRHEFA
                     KLSIPDVSEGRAYDVTGVEQIGPFDITFLKTVHPVVCYAFRIVERATGQVLVFTGDSG
                     YFEGLADFAKEADLFLADVYLYEGNENHMAHLTSKEAGLMASQAGVKKLVLTHMPPVP
                     PEGIDPDNHLEVLRQETQAYAGNIPVDLALPHKSWNLGSLS"
BASE COUNT         2671 a         1738 c         1466 g         2798 t
ORIGIN      
        1 aggtttcaat gccagctaat tgacaaacga agacaagaaa tagaatggtt gataaggggt
       61 agcgaatttt ccaagattga cgactatcca attcaactgc gcaatcatca atagaaataa
      121 taaaatcaat catcgtagtc acctcatcta tagtgtacta ctttgattga ttttgtcatt
      181 atgaatctag ttttctgaaa acaaaagttt cttcatgcgt ttggcgtgga aactagctct
      241 ctcaaaaaag attaaaaata aaggcattct aaagaatcat ttaagaaaaa tttaaggttg
      301 ctatcgtaga atgaaaacat cctaaaaagc ggtttaccta aaccgttggg agaataggtt
      361 ttccccttgt tttccctttt cataaatctc ctgggcacct atcttcttag gtgccctccc
      421 ttgtttaaac ctgaccagtt aaccggctgg ttaggttttt aaatttaaaa agtcaacagc
      481 aaaaatatga tatcacccgc gtgttcttag ctctctcaat tatgggctaa aaacacgcat
      541 caatcaatct attgatgaag tttgctgctt tttgatgatc tttattgtga ctatactggt
      601 aatcctattg attttagttg acttttacgg tagattcgct acaatatata ggattataca
      661 atttaattta aggagagaaa aattacattg ttagattatt atttggaatc cgatattatt
      721 gaccagaaaa tcctactggc tattttgttg acagaaaaag aactgacgat ggcagcagct
      781 tgttctcaaa caactttgac tgctttaaaa ataaagcagt atcttcgaca atttaacgcc
      841 ttatttaaag ggtacttacg tattgagctt attaaatctc gtatttattg tgaggtatta
      901 aatgataagc gagaagcatt tttttatgat atttttgcct tatcagatac tctaaaaatg
      961 ttgacattct tattactaga taatcccaaa cacaagtcaa tcgctgttta tacacgaaaa
     1021 caagggatat cccagtcaaa agcttatcgt cttattcata aattaaaaca ttatttacaa
     1081 gacattggtt taaatattgt agacaatact gttatcggag atgaacttaa aatacggtat
     1141 ttgattgctc ttttacataa agagtacggc attatattat atgatataca acctgcagac
     1201 attgaaacga ttcatgcttt tatctttgca gcacaaaaaa acttacaacc ttctgccttt
     1261 ttagatagac gcttcttgtt ttttgatgtg ctgttgatgt tgacttggaa gcgccatcgc
     1321 tatcctgttc atttgcctca tttagctctt tttgaacatc tcaaatcctt gcctattttt
     1381 gacaatatca aaaccattgc cgtggatgag ctagcacctc gaactcgtgt cacattttcg
     1441 tttgatgatt ttgactatct ctttttaatt tatttaacga ctgataactc cttcttgagt
     1501 gggtactgga caagtcatca gcgacaacaa ctttaccatt taatcaccaa agatcccgat
     1561 tatcatttac taattcatcg tcttcaggca ctagttggaa aatattccga tattcatgag
     1621 catatcccta acttaattcc ttttttcaaa agaactctct acaatttaca aactttaatt
     1681 acctttgatg gttattattt tgatcaatat caaggcaaca tgctactact agataagctt
     1741 gaaaccgtta tcaaagattg gctttatgac acaggccgtc aaggcagtat tagttctggt
     1801 cacttacact tgatgtgttt ataccttgag cagatcttgg aaagctctat tgctccaatc
     1861 aatattactg ttattgagag ccaagaaact gttggtaacg tcatagctaa tttcatcacc
     1921 tcaactatcc cttcttacaa agttgaactg tcacgggtca atatcttatc tgataatatt
     1981 tatccctatg ataaacctgt agatctggtt gtgaccagtc aaaagctctt gcctttttta
     2041 aaagaactcg gtgtttttcc caaagagacc cgcttgtttg gtttgagttt ggactgtatc
     2101 caacagcagc gcgaagattt aatcaaaacc atcttagcct tgcaccagaa ccactaccaa
     2161 aaacgtttag aagaactttg gagaattcct tcttaatttt ttgacagacc ctcctagata
     2221 ctttcacagt ctaggagggt ctttaaggtt gtcacttgca accttgataa gctctctttt
     2281 taaccattgc tatttgccta gaaacagaac tggaactgtt ttagcggttc aactcctctt
     2341 gtaggatctt cttccctttt ttgaaggtct tatctgaaat ttggcataat aaagtgcgct
     2401 cccatgttct ggaagcgcac tttattatcg cttttactta accttgtatc cacttgatga
     2461 tggaagtgcc gccccaattc ttagattgct tggctaactt ttaagctctt gtacaaggaa
     2521 gaagtctttt tgactatctg ttgattagag gcgagcatta agctctgctg ataatgcttc
     2581 aaaacctggt tttccaagaa gcgcaaacat gttacgtttg taagcttcaa cacctggttg
     2641 atcaaatggg ttaacagcat tcatgtagcc tgaaaccgcg atagcaagct caaagaagta
     2701 aatagtgtaa ccaagggtaa attcgtcttg agctggaaga gtgacaaaca tgtttggcac
     2761 gccgccatct gtgtgggcaa gaagcacacc atctgtagct ttcttgttaa cgaaatcaac
     2821 gtctttacct tgaaggtaac caagtccatc taggtcttct gctaattcag gaatgataac
     2881 atttttacga ggtttatcaa cacgaataac tgtttcaaag aggttacggt aaccttcttg
     2941 gataaattga ccaagtgagt gtaagtctgt tgagaagtta gctgaagttg ggtaaatccc
     3001 tttttggtct tttccttctg attcaccggc taattgtttc caccattctc caaagtattg
     3061 aagagatggc tcgtagttag ccaagatttc agtgatgtag cctttacggt aaagcacatt
     3121 acgaacagca gcgtattggt aagcaatgtt ttcagagatt ttgtctgaag aaaggtcttt
     3181 acgcgctgca tttgcacctt ccataagagc tgtgatatct gcacctgatg cagcaattgg
     3241 aagcaaacca acagcagtca agactgagaa acgaccacca acgttgtctg gcaccacaaa
     3301 tgtttcccag ttgttagcgt cagcttctac cttaacagca cctttcactt tgtctgttgt
     3361 agcgtagata cgtttgttag cttcttcttg accatatttt ttaaccaaaa gttctttgaa
     3421 gacacggaaa gcaatcgctg gctcagtagt tgttcctgat ttagaaatca cgtttactga
     3481 gaactctttg tcttgaacgt actctacaag gtctgctaag taagttgatg aaatagagtt
     3541 tccagcgtaa agaatttgtg gggctttgcg ctctttggca gtttgtaagt tagcaaaatg
     3601 atgatttaaa aaatcaatcg ctgctttggc accaaggtat gacccaccga taccgatcac
     3661 cacaagaact tcgctgtctg acttaatttt ttcagcagca gtcaagatac gtgcaaattc
     3721 ctctttgtca tagttttctg gaagatcaag ccaacccaag aaatctgaac ctggtccagt
     3781 tccttcacgc aataacttgt cagcttctgt gacttggcct tgtaaaaaat caatttcatg
     3841 ctgtcccgca aatgactcga ggacttttga ataatcaaat gtaatatgtg acatgagttt
     3901 cgctccctta aaattttaag tttcattagt atgataacct tttctatcta atatttcaat
     3961 ggtttttagt ggttttcacc tgtgaaaaag ctaggttttt tgaaaaggtt ttcgtttttt
     4021 cagacctaaa acatgtgtca atgcaagcat attctcatca tagtggtttt ttaagagaaa
     4081 ataggatttc atatttccta aaaatatcat gaaaaaaggc cctgaggccg ctttaccgta
     4141 ataacttatt ttaaggattt tatcatttca atacgggtaa taaccccatc atgcatatga
     4201 tcaatgcata aagccacacc tacaaaggca attgaagccc caatcacctg taaatacttt
     4261 ctcatgctat cttcttaaca ttacttactc tcattatcta aaaatggcaa tcatttgaat
     4321 acttaaaaag caaaaggggg gggagaatag cactagtacg tttttaagta gatatcaaaa
     4381 tgactaattt cttcactatt aaaaatctta ttatctttat accttttaaa gaaactgtct
     4441 cgacttaaaa gcttatcatc cttattaaat aaatcaactt cataatgttt actatcttta
     4501 gtagcaagga aaaggctccc cgatgtataa cgcgattccg aatcatagat attgtatttt
     4561 tccattagaa attttcttat tttaaaatca aattcctgta aagtaaactg tttctttttt
     4621 atgcgaacaa cctcagaggg caaataagtt tcttttccat cttgatttaa aattcctaca
     4681 aactgtaatg atttatttcc ttgattcgaa tgttttacaa taccaccata aatattatct
     4741 acataaggtg gggaaaaatt ataagggaga ccaaaaacat cgaccttatc cccttttata
     4801 aactgatcag taatgcctaa agatacaata gaatcaacat aaagaataca ttccgatctt
     4861 ttttgtttag tattaatatg tatgctattt gtagtaacaa atgcaatttc tacattttca
     4921 taatcgcatg gggtaatatt ataggcaaac cttaaacttc tttttaaatc ttttaaattt
     4981 tcatctgcat aagctaattg acttccatag ctaaaaacac atgataatat gataattgtc
     5041 aaaatgtttg ttttcattta aaacttttta tgtcctgttt tatacctctt aacctaaata
     5101 aaaatatcaa tcggtttcaa caactgatga cactatagaa ggaagagatt actttagtaa
     5161 agtcaacaaa aaaagagcac acacccgaaa atcgcttagg gctgctggat tcctcccctg
     5221 acccacttca cgcacaagtg ttgctccgcc tattattata tcatattacg gacagtttgc
     5281 aagttttcca actaagtaaa atcactttca agtgagcata gcttcatttt gggacaagat
     5341 ggactgaata cctagaaaaa tcatgaccct ttccaaacac gattttgctt tttgaaaatc
     5401 tttccctctc cccattccag ctattctcta tgttataata agattattag attagaatgg
     5461 gagttatgat gaaacaaaag attaaaatct taacagtaat tggccttatg acagttggta
     5521 tgtctgcgtg tcacaacact agcaaacctt ctaataccga ttctgttttt tctttaaccg
     5581 gaaaaaagcg ccaacaaatc gtcaaacaag taagacaacg gtattatttc caacaattat
     5641 ccaaaaccga acaagaaaat tacctcactt tatacgatag tctagcacag tttcgtgaaa
     5701 tcatcagttt gacgccggtt tctaaaaaaa gcttaattaa aacgattgac gcctttgtga
     5761 tggataatcc cgaattttac tggattacat ctgccgatta tcgatttgag ttttctgatc
     5821 aaacggtctt tgtcaccttt cctatcccag aagatgccaa gaacgtttat caagacttgc
     5881 aagcaatcgg taatgatatc gtcgcaaaca cgccatcaaa ggatcgctat gagcaggtca
     5941 aatattttta tgaggtcatc attcgagaca cagactacaa taaaaaagcc tttgaagcct
     6001 atcaatcagg cagtcaatcc caggtcgctt ctaatcaaga cattaaaagt gtttttattg
     6061 atcatttatc tgtttgtaat ggctatgccc aagcctttca gtttctttgt caaaaagctg
     6121 gcattccagt cgcctatatc cgtggaactg gtacatctca gcaacctcag caatcttttg
     6181 cacatgcatg gaatgctgtt caaattaata acacttatta tggcgttgat gtcacttggg
     6241 gtgaccctgt ttttgataac catctctcac atcaaaagca aggaactatc aattacagtt
     6301 ttttatgcct accagattat ctaatggcct tatcacatca gccaagcaaa gacattgctt
     6361 ttaatactaa agaacgtttt gaaaatgttt ggactatacc ttcttgtaca gatgattctt
     6421 tgctttattc taaacgtcat caaagctata tctcaacgtt tgacaatgac gccatccttg
     6481 caagccttga aaatcagttg ttaaatagac aagagccact gtctctacaa tttgctcatc
     6541 aagatgatta tcaacaaatg gtaactgatt taacgacgaa tcaaacaggt tatcataacc
     6601 ttttcaatca gtattggaat aattatacgg gctttaccta tggactctta ccagagactc
     6661 ttagtatttc atttgctagt aggaactagg tttagacaca tgaaacacaa agttaagatg
     6721 attatctttg tcaaacatta caagaaattg atatactaac acaattgaag gcgattatta
     6781 ttctaaccgt cttcaataga tataaccttt tcagattgag cacatctaat gcttgtaaca
     6841 ttagcaacag tgatgagctc ttttttaatg ctttagcaaa atatatcagc taagacaaaa
     6901 gattaaataa ttcaagccat gcctataagt cacttgtttt taatccctgt gtcagtatca
     6961 atctacgcca cagataactg acagctctga gccctagaag tctgagcttg ataagtttac
     7021 taaaaacaaa aataaggtga gccattacta tctgttttta gatagtaaat ctttttaaga
     7081 atgaccaact catctagtca tcacgtgttt tatttttcga ttgttttcat ctcgattggg
     7141 tctgaaaaag agcgcttcta gtacttggaa acgccctttt gtatattcta tcttcgtatg
     7201 ttgaatctaa caaccattgg aaaaatggtc taacgtttca ctcagtattt tgacaggcta
     7261 cccttcacca aaaatgcatg ttataacaaa ctccttaaaa ctctagtcaa agggatctga
     7321 ctgttccttg atcaaatgct tagctatttt tttgcgctct cgcccttggc ggaaaaaggt
     7381 ttgcatgatg ttagcacagt ctgctgctag caggcctcgt tcaacttgca cgcgatggtt
     7441 taagcgctca tctgtcaaaa tctggtaaag gctgtctgct ccaccaaatt tttgattgct
     7501 ggctccataa ataacatgcg gaatccttgc tagcccaatg gcaccactac acatcacaca
     7561 aggctcgatg gtcacaaaga gtgtggtatc taataaacgc cagttgcctt catgagcgtt
     7621 agcctcgtta atagccatca tttcagcgtg cataatggct tggttactct cttcacgcgc
     7681 attgtgtcca cgaccaataa tctcaccatc cttgacaatg acacagccaa tggggatttc
     7741 agctttctgt aaggattttt cggcttcttt taaagcctct tgcatgaaat aagtttgctc
     7801 ttctaaacta tatggcatca agaaagactc cccaagttcc aagatttgtg tggtaaggca
     7861 aggtcaacag gaatattacc tgcataggct tgtgtttctt gcctcaacac ttccaagtga
     7921 ttgtcaggat caataccttc tggaggaacc ggtggcatgt gggtcaagac aagcttctta
     7981 acacctgcct ggctagccat tagtcctgct tccttactgg tcaaatgggc catatgattt
     8041 tcattgcctt cgtataagta aacatctgct aagaaaaggt ctgcttcttt tgcgaagtct
     8101 gccaatcctt caaaataacc actgtctcca gtaaagacaa gtacctgacc agtcgctctt
     8161 tctacaattc gaaaggcata acacacaact ggatgaactg ttttaagaaa ggtaatgtca
     8221 aaaggaccaa tctgttcaac tccagtgaca tcataggctc taccttctga aacatcagga
     8281 atgcttaact tagcaaattc gtgtcggtcc tcatcatgcc cataaatggg caatacttta
     8341 ggctgccaca ggtgtttagg ataaagttga aaataatggc gcaacacgcc taaatcggcc
     8401 acgtggtctg gatggtaatg gctaataatc actgcatcca aatcgagagg actgatttct
     8461 ttttccagct cattgagcgc tcgactacca gcatccatca ataactgaaa gccgtcatgt
     8521 cctgtgacca aataagaggt tgtgcctgca tcttgatagg gataggctcc ccaagaccct
     8581 aaggtggtta atttcatctc aaacccttct ttcttgcttt aactcttatt ataacaaaaa
     8641 ttagtgtcaa agaggattga gacccaccaa aaa
//
LOCUS       DAUDYX010000063         8534 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.064, whole genome shotgun sequence.
ACCESSION   DAUDYX010000063 DAUDYX010000000
VERSION     DAUDYX010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8534
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.064"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..1409
                     /locus_tag="VHD02_001663"
     CDS             <1..1409
                     /locus_tag="VHD02_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEQ5498253.1"
                     /translation="ILYANSLNLPLKFISLLFVVSTLLIVFLPKKLEVATIIFILVFG
                     TISAIISPINDIPDEYVHYSRTVYISEGDINLTNNNKKLRISKDVDKLIKQSGKTFIT
                     SNLKATKHSTREYSYPYIKGTNAYYSFSYIPQALGILVGNALDLPILLTYYFGRLCNL
                     ISYAMLAFIAIKLSGSFKQVIAVVTLLPMNIYLAASFNQDGFAIGLVLVTIGLFINLL
                     SSKDKSNYNTKFFLYLVLCGLLVLSKFTYFLLVCLPLFIPNEKFGKNTKLVILKKLGG
                     LLLIFLFAAMWFRLYGQVKTPYVADFLKEVNVSQQVKNMLESPIVYSSIIIRHMVINL
                     INMNNIFQFGALSYGITNLFPLYVCFFFFVYISNASKITINIVEKMGIIFVISAIIGA
                     TVLAMYLTWTPVGSSTVLGVQSRYLIGIIPLVLLLFSSQQQKFKQIEDILSDKLAIHV
                     SLLFILAMLMSTIFRYYH"
     gene            1503..2726
                     /gene="pepT"
                     /locus_tag="VHD02_001664"
     CDS             1503..2726
                     /gene="pepT"
                     /locus_tag="VHD02_001664"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEQ5498254.1"
                     /translation="MKYDNLLDRFIKYVKVNTRSDPDSETTPSTESQEAFALTILKPE
                     MEAIGLQDVHYNPVNGYLIGTLPANNPTLTRKIGFIAHMDTADFNAENVNPQIIDNYQ
                     GGDITLGSSNYKLDPKAFPNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTS
                     QPQIEHCDIKVAFGPDEEIGVGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALE
                     VTFLGRNVHPGTAKDQMINALELAIDFHEKLPAKERPEYTDGYQGFYHLTGLTGTVEE
                     ARASYIIRDFEEASFEARKVKVENIAQSMNAHLGTKRVLVELNDQYYNMKKVIEKDMT
                     AIELAKEVMEELAIKPVIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLQT
                     MERAVDVIIGLVCKA"
     gene            2767..3255
                     /locus_tag="VHD02_001665"
     CDS             2767..3255
                     /locus_tag="VHD02_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEQ5498255.1"
                     /translation="MIKLFGKIRYHWQPELSWSIIYWSIAFAPIFVGLSLLYERTEIP
                     SRVFILFAIFAVLVGIGLHRYFIIENNGILRIVSFKLFGPRKLLISTITKIEVTKSTL
                     CLHVEDKSYLFYMRKWPKKYFLDALAVNPYFQGEVILSDNFIKLDYFEVYQHDKKALT
                     RG"
     gene            complement(3242..3439)
                     /locus_tag="VHD02_001666"
     CDS             complement(3242..3439)
                     /locus_tag="VHD02_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEQ5498256.1"
                     /translation="MKVSIIPEKCIACGLCQTYSSLFDYHDNGIVTFSSSSETSQSIC
                     PSDKDAILAVKSCPTKALTLE"
     gene            3488..3964
                     /locus_tag="VHD02_001667"
     CDS             3488..3964
                     /locus_tag="VHD02_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEQ5498257.1"
                     /translation="MAKEPWEEKIVDDTIGTRTRKSRNAFISTPWLTALLSVFFVIIV
                     AILFIFFYTSNSGSNRQAETNGFYGASTHKKTRKASNAKKTSSSSTTTDTTPSSEETL
                     ASSEGTGETLTVLAGEGAASIAARAGISVEQLQALNPEHMTQGYWYANPGDQVTIK"
     gene            3979..4659
                     /locus_tag="VHD02_001668"
     CDS             3979..4659
                     /locus_tag="VHD02_001668"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEQ5498258.1"
                     /translation="MKAIKIAIDGPASSGKSTVAKIIAKNLGYTYLDTGAMYRSATYI
                     ALTHGYTGKEVALILEELEKNPIFFKKAKDGSQLVFLGDEDVTLAIRQNDVTNNVSWI
                     SALPEIREELVHQQRRIAQAGGIIMDGRDIGTVVLPDAELKIFLVASVEERAERRYKE
                     NLEKGIESDFETLKEEIAARDYKDSHRKVSPLKAAEDALIFDTTGVSIDGVVQFIQEK
                     AEKIVDMS"
     misc_feature    4683..4806
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            4821..5351
                     /gene="infC"
                     /locus_tag="VHD02_001669"
     CDS             4821..5351
                     /gene="infC"
                     /locus_tag="VHD02_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEQ5498259.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQSLADA
                     SNVDLVLIQPQAVPPVAKLMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLADFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPISDKK"
     gene            5393..5590
                     /gene="rpmI"
                     /locus_tag="VHD02_001670"
     CDS             5393..5590
                     /gene="rpmI"
                     /locus_tag="VHD02_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEQ5498260.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAFTSHRFHGKTKKQRRHL
                     RKAGLVSSGDFKRIKAMVTGL"
     gene            5649..6008
                     /gene="rplT"
                     /locus_tag="VHD02_001671"
     CDS             5649..6008
                     /gene="rplT"
                     /locus_tag="VHD02_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEQ5498261.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVAD
                     AAAFTALADAAKAKLGK"
     gene            complement(6299..8509)
                     /locus_tag="VHD02_001672"
     CDS             complement(6299..8509)
                     /locus_tag="VHD02_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEQ5498262.1"
                     /translation="MTSLGKNDKEVATVKKFKTLITGFINTRLGFIITLLFCYWIKTL
                     WAYHTDFSLDLGNIYQVFLTIINPIPLAFLLLGGALYVKNTRAFYICSWVVYIILNIL
                     LISNSIYYREFSDFITVSAMLASSKVSAGLGDSALNLLRIWDIIYILDFIILISLSIT
                     KKIKNDQRPFNKRAAFAITALSSLLLSINLFLAEIDRPELLTRGFSNTYIVRALGLPA
                     FTLYSGNQTYQAQKERNGATAEELIDVKTYVKGHYAAPDPQYFGIGKGKNIIVLHLES
                     FQQFLIDYKLKEGDKEYEVTPFINSLYHSNATLAFPNFFHQVKAGKTSDAETMMENSL
                     FGLNSGSFMVNYGGENTQFATPSILTQKGGYTSAVFHGNVGTFWNRNNAYKQWGYNYF
                     FDSSYFSKQNSKNSFQYGLNDKYMFKDSIKYLEQMQQPFYTKFITVSNHYPYTSLKGE
                     SSEEGFPLAKTDDETINGYFATANYLDAALKSFFDYLKATGLYDNSIFVLYGDHYGIS
                     NSRNSSLAPLLGKDSETWSEYDNAMLQRVPYMIHIPGYTNGSIKETFGGEIDALPTLL
                     HILGIDTSQFVQLGQDLLSPQNSQIVAQRTSGTYMTPEYTNYNGRLYNTQTGLEITNP
                     DEMTIAKTKEIRSAVAQQLAASDAIQTGDLLRFDTQNGLKAIDPNQFIYTKQLKQLKD
                     ISAKLGSESTSLYSKNGHKSTQKLFKAPSYLELNPVEADAATSELKEDNTKNRE"
BASE COUNT         2783 a         1383 c         1634 g         2734 t
ORIGIN      
        1 ttattttata tgccaatagt ttaaatctac ctttaaaatt tattagtctt ctttttgtgg
       61 tgagcacttt actgatagtg tttttaccaa aaaagttaga ggttgctacg atcattttta
      121 ttttagtatt tggaacgata tctgctatca tatcacctat aaatgatatt cctgatgaat
      181 atgttcatta ttcaaggaca gtttatatct cagaaggtga tattaatctt accaacaata
      241 acaaaaagtt aagaatatcc aaagatgttg ataagcttat taaacagtca ggaaaaacat
      301 ttataacatc aaacttgaaa gcaactaaac acagcacaag agagtatagc tatccttaca
      361 tcaaaggaac taatgcatat tatagttttt cttatatacc acaagctttg ggtattttag
      421 taggaaatgc gctggattta cctatacttt taacatatta tttcggaaga ttgtgcaacc
      481 ttattagcta cgctatgctt gcttttatag ctatcaagtt gtctggttct tttaaacaag
      541 tgatagctgt tgttacttta ctacctatga acatttattt agcagcatct tttaatcaag
      601 acggctttgc tatcggttta gttttagtaa caattggatt gtttattaac ttgctctcat
      661 caaaagataa atctaactat aataccaaat tcttccttta tttagttcta tgtgggttac
      721 tagttctttc aaaattcaca tactttttat tagtatgcct tccattattt attcctaatg
      781 aaaaattcgg aaaaaatacc aagttagtca tcttgaaaaa attgggagga ctattactca
      841 tttttctttt tgctgcaatg tggtttagac tttatggaca ggttaaaacc ccatatgtcg
      901 cggattttct aaaagaagta aacgttagcc agcaagttaa aaatatgcta gaatccccta
      961 tagtctatag cagtattatt attagacata tggtaattaa tctgattaat atgaataata
     1021 tttttcagtt tggcgcatta agctatggta taacgaacct tttcccgctt tatgtgtgtt
     1081 tcttcttttt tgtttatatt agtaatgcta gtaaaataac tattaatatt gtagaaaaaa
     1141 tgggtataat ctttgttatt tcagcgatta taggtgcaac tgtattagct atgtacctga
     1201 cttggacacc agtagggtcc tcaactgtct taggtgttca aagtcgttat ctgataggaa
     1261 ttatcccatt agtgttgctt ctttttagta gtcaacagca aaagttcaaa caaatagagg
     1321 atatcctttc tgacaaactg gctattcatg tttcgctttt gtttattctc gcaatgttaa
     1381 tgagtactat ttttagatat tatcattaaa ctcttaatta gaggctttaa aaaacttagc
     1441 ttgaataagt attttaaagc cttttatgct ataattaaat tgagaaatta ttgaggtggc
     1501 ctatgaagta cgataacttg ttagatcgat ttataaaata tgtcaaagtg aacacaagaa
     1561 gcgatccaga cagtgagact actcctagta cggagagtca agaagcattt gccttaacga
     1621 ttttaaagcc tgaaatggag gcaattggtt tgcaagatgt tcattataat ccagttaatg
     1681 gctatcttat tggaactctg cctgccaata atccaacctt gacgcgtaaa attggtttta
     1741 tagcccatat ggatacggct gatttcaatg ctgaaaatgt taatccgcag attattgaca
     1801 attatcaagg aggggacatt acacttggca gttctaatta taagttggat ccaaaagcat
     1861 ttccaaatct taataattat atcggtcaaa cattgattac cactgatggg actacacttt
     1921 taggggcaga tgataaatct ggtattgctg aaatcatgac agcaatagaa ttcttaacat
     1981 cacaaccaca aatagagcac tgtgacatta aagtggcttt tggccctgat gaagagattg
     2041 gggtcggagc tgataagttt gaggtggctg attttgaggt ggattttgct tatactatgg
     2101 atggcggtcc tttaggcgaa ttgcaatatg aaactttcag cgctgcagct ctagaagtga
     2161 cttttttagg cagaaatgtt catcctggta cggctaagga tcaaatgatt aatgcccttg
     2221 agttagcaat agattttcat gagaagctac ctgctaaaga gcgacctgaa tacacagatg
     2281 gttatcaagg cttctatcac ttaacgggac taactggaac agttgaagag gcacgcgcat
     2341 cttacattat tcgtgatttt gaagaagcat catttgaagc tagaaaagtg aaagttgaaa
     2401 atatagctca atcgatgaat gctcatctag gaacaaaacg tgtcttggta gagcttaatg
     2461 accaatacta caatatgaaa aaagttatcg aaaaagatat gaccgctatt gagttagcta
     2521 aagaagtaat ggaagagctt gctatcaagc ctgtaatcga acccatacgt ggaggaacag
     2581 atggctctaa aatttcattt atgggtattc ccactcctaa tatttttgca gggggtgaga
     2641 atatgcacgg gcgttttgag tttgttagct tacaaacgat ggagagagct gtcgatgtca
     2701 ttattggact tgtttgcaaa gcctaattat catgtaattg atatttagtg gaaggggctt
     2761 ataaaaatga ttaaactatt tggtaaaata aggtatcact ggcagccaga gttgtcttgg
     2821 tctattattt attggtctat tgcgtttgca cctatttttg taggtttatc tttgttgtat
     2881 gagcgcactg aaatacctag tcgggttttt attctatttg ctatttttgc tgtcctagtt
     2941 ggcattggat tacatcgtta tttcattatt gaaaataatg gtattttaag aatcgtatct
     3001 tttaaattgt ttggtccacg taagctactt atttctacta ttactaagat tgaggtgacc
     3061 aaatcaacac tttgtctgca cgttgaagat aaaagttatc tcttttatat gcgcaaatgg
     3121 cctaaaaaat attttcttga tgccttagcg gttaatccat acttccaagg agaagttata
     3181 ttaagtgata actttattaa attagactat tttgaagttt atcagcatga taaaaaagcc
     3241 cttactcgag ggtaagagct ttagttgggc aagatttgac agctaaaata gcgtccttat
     3301 ctgaaggaca aattgactga cttgtttcag atgagcttga aaatgtgacg ataccattgt
     3361 catgataatc aaaaagtgat gaataggttt ggcatagacc acaagcgatg catttttctg
     3421 gaatgataga tactttcata actattattc taatatgatt ttgttaaatt aacaaggggg
     3481 gacgatcatg gctaaagaac catgggaaga aaaaattgtt gatgatacta tagggacacg
     3541 aacacgtaaa tcaagaaatg ctttcattag cacgccttgg ttgactgctt tattaagtgt
     3601 attctttgtc atcattgttg ctatactttt tattttcttc tatacatcaa atagcggtag
     3661 taatagacaa gctgaaacaa atggttttta tggagcatcc actcataaaa aaacaaggaa
     3721 agcttctaac gctaaaaaaa catcaagtag ttcaacaact acagacacaa caccttctag
     3781 cgaagaaaca cttgcttcta gtgaaggaac tggcgaaacc cttactgtat tggcaggtga
     3841 gggggcagct tctattgcag ctcgtgcagg tatttctgtg gagcagctac aagcacttaa
     3901 tccagagcac atgactcaag gatattggta tgccaatcca ggagatcaag tcactattaa
     3961 ataattgaag aggaaaaaat gaaagcaatt aaaattgcga ttgatgggcc agcttcaagt
     4021 ggaaaaagta cagtagccaa gattattgca aaaaatcttg gctatactta tctagatact
     4081 ggtgctatgt atcgctcagc aacttatatc gcactgacac atggctatac gggtaaagaa
     4141 gtagccctta ttttagagga acttgagaaa aatcctattt tctttaaaaa agcaaaagat
     4201 ggctcccaat tagtttttct tggagatgag gacgtgacct tagccattag gcaaaatgat
     4261 gtgactaaca atgtttcttg gatctcagct ttgccagaaa ttagagaaga gctggttcat
     4321 caacaaagac gcattgcaca agctggtggc attatcatgg atggtcgtga cattggaacg
     4381 gttgttctcc cagatgcgga actaaaaatc tttttagtgg cttctgtcga agagcgggca
     4441 gagcgccgct ataaagaaaa cctagaaaaa ggaattgaat cagattttga gacgctaaaa
     4501 gaagagattg cagctcgtga ttacaaggat agtcatcgta aggtttcacc tctaaaagca
     4561 gctgaagatg cccttatttt tgataccaca ggtgtttcta ttgatggcgt ggtgcaattt
     4621 atccaagaaa aagcagaaaa aatagttgac atgtcctaga gcctatgtta gaatattaac
     4681 aatgagaaaa gtagaagtga gagcttctcg ccttgcgact taggttgtct ggccctaaca
     4741 tgtcgaattc ctatggaata ttatgtgtgc gggcttgatt tatcaagtcc gctttcgttt
     4801 ttctctaaaa aaataaagag gtgaagatca tagctaaaaa ggatctattc attaatgatg
     4861 aaattcgcgt tcgcgaagtt cgtctcgttg gtcttgaagg tgaacaatta ggtattaaac
     4921 cattgtctga agcgcaatca ttggctgatg cttcaaatgt tgatttggtt ttaatccagc
     4981 cacaagctgt tcctcctgtt gccaaactta tggactatgg aaagttcaaa tttgagtatc
     5041 aaaagaaaca gaaagaacaa cgtaaaaaac aaagcgttgt gactgtgaaa gaagttcgtc
     5101 tcagtccagt tatcgataaa ggtgactttg aaacaaaact tcgtaacggt cgtaaattcc
     5161 ttgaaaaagg aaataaagtt aaggtttcta ttcgtttcaa aggacgtatg attactcata
     5221 aagagattgg tgccaaggta ctagctgact ttgctgaagc gactcaagat attgctatca
     5281 ttgagcaacg agcaaaaatg gatggacgcc aaatgtttat gcaacttgca ccaatttcag
     5341 acaagaagta attgtcacag tttcattata tctttaagag gagaatacta aaatgccaaa
     5401 acaaaaaaca caccgcgcat cagctaaacg ttttaaacgt acaggttcag gcggtttgaa
     5461 acgcttccgc gcctttacat cacaccgttt ccacggaaaa actaaaaaac aacgtcgtca
     5521 tcttcgtaaa gctggtttgg ttagctcagg agatttcaaa cgtatcaaag caatggttac
     5581 tggtctttaa ttttaaaggc agtagacatt tacgagtatt ataagaatta tttgaaggag
     5641 aactattaat ggcacgtgtt aaaggtggcg ttgtttcacg caaacgtcgt aaacgtattt
     5701 taaaattagc taaaggttac tatggagcaa aacacatctt gttccgtact gcaaaagagc
     5761 aagtgatgaa ctcttactac tatgcatacc gtgaccgccg tcagaaaaaa cgtgatttcc
     5821 gtaaattatg gatcactcgt atcaatgcgg ctgctcgtat gaacggtttg tcatactcac
     5881 aattgatgca cggtttgaaa cttgctgaaa tcgaagtaaa ccgtaaaatg cttgctgatt
     5941 tagcagtagc tgatgcagca gctttcacag ctcttgcaga tgcagccaaa gcaaaacttg
     6001 gcaaataaga aacgctatac ctagaaaata actttttatt acctatagaa agttataaag
     6061 aaacaaaaat gaaggagacg atggacgtct tctttttatt atactcaata taataaaaag
     6121 aagtttccca tgttttatta ccagtaatgt gggatattta gatggtagca agaagtttta
     6181 tagttgattt gttttcttta ggtctaatta gcatattttg attactgata aacttgaata
     6241 tcgccaataa aaaagaagca aaattattat tttgcttcta gtcagatcat taaattgatt
     6301 attctctatt tttggtgtta tcctctttta gttcagaagt tgccgcgtca gcttctacgg
     6361 gatttagttc taagtaagat ggtgctttaa aaagtttctg agttgattta tgaccatttt
     6421 tactgtataa acttgttgac tctgatccga gttttgctga aatatctttc agttgtttta
     6481 actgcttagt gtagataaac tggttaggat caatagcttt tagaccattt tgagtatcaa
     6541 agcgcaggag atcacccgtt tgaatagcgt cgctagctgc tagttgttga gcaacagcag
     6601 agcgaatttc cttagttttg gctatagtca tttcatcggg attagtgatt tctaaacctg
     6661 tttgcgtgtt gtacagacgt ccattatagt tagtgtattc aggagtcata taagtcccag
     6721 atgttcgttg tgccacaatc tggctatttt gaggtgataa taaatcttgt cctaattgaa
     6781 caaactgact agtgtcaata ccaagtatgt ggagtaaagt aggaagagca tcgatttcac
     6841 cgccaaaggt ttctttgata ctaccattcg tataacctgg aatatggatc atatacggga
     6901 ctcgttgtag catagcatta tcatattcag accaagtttc agaatcttta ccaagaagtg
     6961 gagcaagact agaattacga gaatttgaaa taccataatg gtcaccatat aaaacaaaaa
     7021 tagaattgtc gtacaaacca gtggctttca agtaatcaaa aaaagattta agggcagcgt
     7081 ctaaataatt ggcagtagca aagtaaccat tgatcgtttc atcgtctgtt ttggctaaag
     7141 gaaaaccttc ttcactactt tcgcctttta gactagtata aggatagtga ttactcacag
     7201 tgataaactt ggtataaaaa ggttgttgca tctgttcaag atattttatg gaatccttaa
     7261 acatgtactt atcatttaac ccatattgaa aagagttctt actattttgt ttagagaagt
     7321 agctagaatc aaaaaaataa ttatagcccc attgtttata agcattattg cgattccaga
     7381 aagttccaac gttaccatga aagacagcgc tggtatagcc acctttttgg gttaaaatac
     7441 ttggagtagc aaattgtgta ttttcaccac cgtagttcac cataaaagaa ccactattta
     7501 aaccaaataa ggaattctcc atcatggttt cagcatcaga agttttacct gctttaacct
     7561 gatgaaaaaa gttagggaat gctaaggtag catttgagtg ataaagcgag ttgataaaag
     7621 gcgttacctc atattcttta tctccttctt ttagtttata atctattaag aattgttgaa
     7681 agctttctaa atgaagaaca ataatatttt tgccttttcc aattccaaaa tactgaggat
     7741 caggtgctgc ataatgtcct ttgacataag ttttaacatc aattaattct tctgcagtag
     7801 ctccatttcg ttctttttga gcttgatagg tttgattgcc gctatagagg gtaaacgctg
     7861 gtagtcctaa agctctcaca atataagtat ttgaaaagcc acgtgttaat agttccggac
     7921 gatcaatttc tgctaaaaaa agattaatag aaagcaatag actagataaa gcggtgatgg
     7981 caaatgctgc gcgcttgtta aaaggacgtt ggtcattttt gattttttta gtgatagata
     8041 aactaattag aataataaaa tcaagaatat agataatatc ccagatgcgt aaaaggttta
     8101 aagcagagtc ccctaatcca gcggagactt tactactagc tagcatggca ctcactgtaa
     8161 taaagtctga aaattctcgg taatatatag aatttgatat caagagaatg ttaaggataa
     8221 tataaaccac ccaagaacag atataaaagg ctcgggtatt cttaacgtaa agagcacccc
     8281 ccaaaagtaa aaaagctagt gggattggat tgataatagt tagaaagact tggtatatat
     8341 ttcccaaatc tagtgaaaag tctgtatgat atgcccacag ggtctttatc cagtaacaaa
     8401 agagtagagt aattataaaa cccaatcttg tgttgataaa acctgtgata agtgttttaa
     8461 atttcttcac agtagcaact tccttatcgt tttttcctaa agatgtcatc tccaaataaa
     8521 atctcagatt aaat
//
LOCUS       DAUDYX010000064         8417 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.065, whole genome shotgun sequence.
ACCESSION   DAUDYX010000064 DAUDYX010000000
VERSION     DAUDYX010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8417
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.065"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            513..830
                     /locus_tag="VHD02_001673"
     CDS             513..830
                     /locus_tag="VHD02_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEQ5498263.1"
                     /translation="MIVRNGKNFLTRDAADQYYTIGGTSLVGEKTHETVLRETLEEVG
                     IRAKVNQLAFMVENHFDIDDVFWHNIEFHYLVSPLEDPKLEMIENASDRFMNGFQSIN
                     WIK"
     gene            954..2852
                     /gene="mnmG"
                     /locus_tag="VHD02_001674"
     CDS             954..2852
                     /gene="mnmG"
                     /locus_tag="VHD02_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEQ5498264.1"
                     /translation="MTHEFTESYDVIVIGAGHAGVEASLATSRMGCKTLLATINLDML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KSLYAREMKHTVEKQANLTLRQTMIDDILVEDGRVVGVLTATGQKFAAKAVVVTTGTA
                     LRGEIILGELKYSSGPNNSLASVTLADNLKKLGLEIGRFKTGTPPRVKASSINYDQTE
                     IQPGDDKPNHFSFMSKDADYLKDQIPCWLTYTNQTSHDIINQNLYRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRDTEEVYVQGLSTSLPEDVQKDLIHSIKGL
                     EKAEMMRTGYAIEYDIVLPHQLRATLETKLISGLFTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGRDIGLVDDERWKAFEIKKNQFDNELKRLNSIKLKPVKATNDRVQELGFKPLTD
                     AMTAKEFMRRPEIDYATAVSFVGPAAEDLDAKIIELLETEIKYEGYIRKALDQVAKMK
                     RMEEKRIPANIDWDAIDSIATEARQKFKKINPETIGQASRISGVNPADISILMIYLEG
                     NGKAHRKY"
     gene            2942..4918
                     /locus_tag="VHD02_001675"
     CDS             2942..4918
                     /locus_tag="VHD02_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEQ5498265.1"
                     /translation="MKKFRFETIHLIMMGLILFGLLALCVSIMQSKILILLAIFLVLL
                     FVVALLWYQKEAYQLSDLAHIELLNEQTEDNLKTLLDNMPVGVVQFDQETNAVEWYNP
                     YAELIFTTEEGFIQNSLIQQIITEKRREDISQTFEVSGNKYTSYIDVSSGIFYFFDSF
                     VGNRQLADASMLRPVVGIISVDNYDDITDDLSDADTSKINSFVANFIDEFMESKRIFY
                     RRVNMDRYYFFTDFKTLNDLMDNKFSVLEEFRKEAQDAQRPLTLSIGISFGEENHSQI
                     GQVALENLNIALVRGGDQIVIRENADHTNPIYFGGGSVSTVKRSRTRTRAMMTAISDR
                     IKMVDNVFIVGHRKLDMDALGSAVGMQFFAGNIIENSFAVYNPDEMSPDIERAIERLQ
                     ADGKTRLISVSQAMGLVTPRSLLVMVDHSKISLTLSKEFYEQFQNVIVVDHHRRDDDF
                     PDNAILTFIESGASSAAELVTELIQFQNAKKRLNKIQASVLMAGIMLDTKNFSTRVTS
                     RTFDVASYLRSKGSDSVEIQNISATDFEEYKQINEIILQGERLGDSIIVAAGEKNHLY
                     SNVIASKAADTILSMAHVEASFVLVETASHKIAISARSRSKINVQRVMEKLGGGGHFN
                     LAACQLTDISLPQAKHLLLKTINMTMKETGEVES"
     gene            4915..5367
                     /gene="rplI"
                     /locus_tag="VHD02_001676"
     CDS             4915..5367
                     /gene="rplI"
                     /locus_tag="VHD02_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEQ5498266.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATSQSI
                     GELKGKQKAEEKAQAEILAEAQAVKAVLDEDKTRVQFQEKVGPDGRTFGSITAKKISE
                     ELQKQFGVKVDKRHIVLDHPIRAIGLIEVPVKLHKEVTAEIKLAITEA"
     gene            5397..6764
                     /gene="dnaB"
                     /locus_tag="VHD02_001677"
     CDS             5397..6764
                     /gene="dnaB"
                     /locus_tag="VHD02_001677"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEQ5498267.1"
                     /translation="MRLPEVAELRVQPQDLLAEQSVLGSIFISPDKLIAVREFISPDD
                     FYKYAHKIIFRAMITLSDRNDAIDATTIRTILDDQDDLQSIGGLSYIVELVNSVPTSA
                     NAEYYAKIVAEKAMLRDIIARLTESVNLAYDEILKPEEVIAGVERALIELNEHSNRSG
                     FRKISDVLKVNYEALEARSKQTSNVTGLPTGFRDLDKITTGLHPDQLVILAARPAVGK
                     TAFVLNIAQNVGTKQKKTVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWN
                     NVTIAQGALAEAPIYIDDTPGIKITEIRARSRKLSQEVDGGLGLIVIDYLQLITGTKP
                     ENRQQEVSDISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDA
                     DIVAFLYRDDYYRKEGDDAEEAVEDNTIEVILEKNRAGARGTVKLMFQKEYNKFSSIA
                     QFEER"
     gene            6781..7053
                     /locus_tag="VHD02_001678"
     CDS             6781..7053
                     /locus_tag="VHD02_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEQ5498268.1"
                     /translation="MSDAFTDVAKMKKIKEDIRAHEGQLVELTLENGRKREKNKIGRL
                     IEVYSSLFIIEYSDSSDTPGAIDNSYVESYTYSDILTEKTLIRYLD"
     gene            7783..8394
                     /gene="rpsD"
                     /locus_tag="VHD02_001679"
     CDS             7783..8394
                     /gene="rpsD"
                     /locus_tag="VHD02_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEQ5498269.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKIKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVDPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
BASE COUNT         2784 a         1333 c         1717 g         2583 t
ORIGIN      
        1 gattatttca tgattattag tacctattat ctaatactaa atgtgtaaaa aattattgga
       61 aatttctagt tatactttta tggttttgtt atcggcgttc ttacttgtag acttagaaac
      121 aacggtatct gtattttact actttagaag atatcaaaaa atcgtatgat aatactgatg
      181 acatttttat ttctcgcctc gctagattag acatagtgat acttttgtaa tcattttaaa
      241 aacattacaa tagattgaca gagctgtaaa aacattgaca ttactgtaaa ggtctgtgat
      301 gaaatcaata ttgacaatca ctaatcattt ttttaaaata gatagtaaca atcggaatga
      361 tggtcaaagc tcatgaaagt tgtaggtttt tttgtcctgc aatcttctag ggttttggcc
      421 cttttatggt ttagctctag aaatgagatg gctaatagga ctggaattta aaatcactag
      481 aggtcagata actttagttt acgagctagc gcttgattgt tagaaatggt aagaattttt
      541 taactagaga tgcagctgac caatattaca ccattggtgg tacaagtttg gtaggtgaaa
      601 aaacgcatga aactgtttta cgtgaaacct tagaggaagt tggtattcga gctaaggtta
      661 atcaactggc ttttatggtt gagaatcatt ttgatatcga tgatgttttc tggcataata
      721 ttgaatttca ttatttggta agccccttag aagaccctaa actagaaatg atagagaatg
      781 caagcgacag atttatgaat ggattccaat caatcaactg gatcaaatga atcttgttcc
      841 tgagttttta caaaccgagt tggctaaatg gccaggacat attgtccgca ttgaaggcta
      901 aactaaagcg tatgatttta aatgaataga ctgaaataaa ggattttaaa aaaatgacac
      961 atgaatttac agaaagctat gacgtcattg tcataggtgc aggtcatgct ggtgttgaag
     1021 ccagtttggc cactagtcgt atgggttgta aaactttatt ggcaacaatc aaccttgata
     1081 tgttggcttt tatgccgtgt aatccttcta ttggaggatc tgccaaaggg atagtagtac
     1141 gagaaatcga tgctcttggc ggcgaaatgg gtaaaaatat tgataaaacc tatatccaga
     1201 tgaaaatgct taatacaggt aagggaccag ctgtacgcgc tctccgagct caagcagata
     1261 aatccctgta tgcgcgtgag atgaaacata cggttgaaaa acaggctaat ttgactcttc
     1321 gccaaacgat gattgatgat attctagttg aagatggacg agttgtaggt gttttaacag
     1381 ccacaggaca aaagtttgca gctaaggcag ttgtagtgac aacaggaaca gctctgcgtg
     1441 gcgaaattat tttaggagaa ctcaaatatt cttctgggcc taataatagt ttggcatcag
     1501 taaccttagc agataattta aaaaaactcg gcttagaaat tggcagattc aaaacaggaa
     1561 cgcctcctcg tgtcaaagca tcttctatta attacgatca aacggaaatt cagccaggtg
     1621 atgataaacc taatcatttt tcatttatgt caaaagatgc tgattacctc aaagatcaaa
     1681 ttccctgctg gttgacttat actaatcaaa ctagtcatga tatcattaat cagaaccttt
     1741 acagagcacc gatgttttct ggcattgtta aaggtgtagg ccctcgttac tgcccatcta
     1801 ttgaagataa gattgttcgc tttgctgata aagaacgtca tcaacttttc ttagagccag
     1861 aggggcgtga tacagaagaa gtgtatgttc aagggttgtc aacgagtctt ccagaagatg
     1921 tccaaaaaga tttgattcat tcgatcaaag gacttgaaaa ggctgaaatg atgcgcactg
     1981 gttatgctat tgaatatgat attgttttac ctcaccagct acgtgccaca cttgaaacca
     2041 agctaatttc aggactcttt acggctgggc aaactaatgg aacttctggg tatgaagagg
     2101 ctgctggcca aggacttatt gctggaatca atgctgcttt gaaagttcaa ggtaagccag
     2161 aacttatttt gaaacggagc gatgcctata ttggcgttat gattgacgat ttggttacca
     2221 aaggaacctt ggaaccttat cgattgttga catcgcgagc tgagtatcgt ttgattttac
     2281 gtcatgacaa cgctgatatg cgtttgacag agattggtcg cgacattggg ttggttgatg
     2341 atgagcgttg gaaggccttc gaaattaaga aaaaccaatt tgataacgaa ttaaaacgtc
     2401 tgaacagtat caaattaaaa ccagtaaaag caaccaacga tcgtgtgcag gaacttggtt
     2461 ttaaaccatt gacggatgct atgacagcta aagaatttat gcgtcgccca gaaattgatt
     2521 atgcgactgc agtctctttt gttggtcctg cggctgaaga tttagatgct aaaatcattg
     2581 aacttttaga aacagaaatc aagtacgaag gctatattcg caaagcttta gatcaggttg
     2641 ctaagatgaa acgcatggaa gaaaaacgta ttcctgcaaa tattgattgg gatgctattg
     2701 attctattgc aactgaggcg cgtcagaaat ttaagaaaat taatcctgaa acaattggac
     2761 aagctagtcg tatttcagga gtcaatcctg cggacatcag tatcttgatg atttatttag
     2821 agggaaacgg aaaagctcat cgaaaatatt aaatggaaat gaaattgtaa tctcaaagca
     2881 agtggaagct ctgtatttca gggctttttt atggtataat gtgtgcttaa gaggttataa
     2941 gatgaaaaaa tttcgttttg aaaccattca tttgattatg atgggattga ttttatttgg
     3001 actactggca ctttgtgtca gtattatgca atcaaaaata ttaatattat tagctatttt
     3061 tctcgttttg ctatttgttg ttgctctttt atggtatcaa aaagaagctt atcaattatc
     3121 agacttagct catattgagc tcttaaatga acaaacagaa gataacctaa aaacgctact
     3181 tgataatatg cctgttggag tagttcaatt tgatcaagag accaacgctg tagaatggta
     3241 taatccttat gcagaattaa tttttacaac tgaagagggt tttatacaaa acagtttgat
     3301 tcagcaaatt attacggaaa aacgtcgtga agatatttct caaacatttg aagtttctgg
     3361 taataaatac acttcttata ttgatgtatc atcaggaatt ttttattttt ttgatagctt
     3421 tgtgggaaac aggcaattag ctgatgctag tatgttaaga ccggttgttg gcattatttc
     3481 ggttgataat tatgatgata tcactgatga tttatcagat gcagatacct caaaaattaa
     3541 ttcatttgta gctaacttca ttgatgagtt tatggagtca aaacggattt tttaccgtcg
     3601 tgttaatatg gatcgctact atttctttac agactttaaa acattaaatg atttgatgga
     3661 taataaattt tctgtcttag aagagtttcg taaagaagct caagatgctc agcgcccttt
     3721 aacactcagc ataggaattt cttttggtga agaaaatcat agccagattg gacaggtagc
     3781 tcttgagaat cttaatattg cgcttgttcg tggtggtgat cagattgtca ttcgtgaaaa
     3841 tgcggatcat acgaatccaa tttatttcgg gggagggtct gtttctacag ttaaacgttc
     3901 aagaacacgt acccgtgcta tgatgacagc tatttcagat cgaatcaaga tggtggacaa
     3961 tgtttttatt gttggacata ggaaacttga tatggatgca ctaggttcag ctgttggtat
     4021 gcaatttttc gcaggtaaca ttattgaaaa tagttttgcc gtttacaatc cagatgagat
     4081 gagtccagat attgaaaggg ctattgagcg attgcaggct gacggaaaga ctcgtctaat
     4141 cagtgtatct caagcaatgg gtttagtgac tccaagatca cttttggtaa tggttgacca
     4201 ttctaagata tctttaacac tttctaaaga attttatgaa cagtttcaaa atgttattgt
     4261 agttgatcat cacagaagag atgatgattt tcctgataat gctatcttaa cttttattga
     4321 aagtggagca agtagtgctg cggagctcgt cactgagtta attcaatttc aaaatgctaa
     4381 aaaacgttta aataagatcc aagctagtgt tttaatggca ggtattatgc ttgatactaa
     4441 aaatttttct acgcgagtga caagtcgtac ctttgatgtc gctagttact taagaagcaa
     4501 aggaagtgat agtgttgaaa ttcaaaatat atccgcaaca gattttgaag aatataagca
     4561 aataaatgaa attattttac aaggagaacg tcttggtgac agtatcattg tagctgcagg
     4621 agaaaaaaat catctttata gcaatgttat tgctagtaaa gctgcagata cgatactgtc
     4681 aatggctcac gttgaggcta gctttgtatt agttgaaaca gcttctcata agattgctat
     4741 ttcagctaga agtcgtagta aaatcaatgt tcaacgtgtg atggaaaaat taggtggagg
     4801 aggccacttt aatcttgctg cctgtcagtt aacggatatc agtcttcccc aagcgaagca
     4861 cttattattg aaaactatta atatgacaat gaaagaaaca ggagaagtag aatcatgaaa
     4921 gttatttttt tagcagatgt aaaaggaaaa ggtaaaaaag gagaaatcaa agaggtccca
     4981 acaggctatg ctcaaaattt ccttattaag aaaaatttag ctaaagaagc aactagtcaa
     5041 agcatcggtg aattaaaagg aaaacaaaaa gctgaagaaa aagctcaagc tgaaatatta
     5101 gcagaagctc aagctgtcaa ggctgttttg gatgaggaca aaacacgtgt tcaatttcaa
     5161 gaaaaagttg gcccagatgg acgtacattt ggttccatca cagctaaaaa gatttcagaa
     5221 gaattgcaaa agcaatttgg tgtgaaagtt gataaacgtc atattgtttt agatcatcct
     5281 atccgcgcca ttggtctaat tgaagttcct gtgaaattac ataaggaagt aacagctgag
     5341 attaaattgg ctatcacaga agcataatat aaaaaacatc acaagatttg gaggttatga
     5401 ggttgcctga agtagctgaa ttacgagttc aaccccaaga tttactagca gagcaatctg
     5461 ttcttgggtc aatctttatc tcacctgata agctgattgc agtgagagaa tttatcagtc
     5521 cagacgattt ttataagtac gctcataaaa ttatctttcg ggcaatgatt accctcagcg
     5581 atcgtaatga tgccattgat gcaaccacta taagaacaat cctagatgat caagatgatc
     5641 tgcaaagtat tggtggctta tcctatattg ttgaactagt taatagtgtc ccaactagtg
     5701 ctaatgcaga atattatgct aaaattgtag ctgagaaagc tatgttgcgt gatattattg
     5761 ctaggttgac agaatctgtc aacctagctt atgatgaaat tttaaaacca gaagaggtta
     5821 tcgctggagt tgagagagct ttaattgaac tcaatgaaca tagtaatcgt agtggatttc
     5881 gcaaaatttc agatgtgcta aaagttaatt acgaggcttt agaagcacgt tctaagcaga
     5941 cttcaaatgt tacaggttta ccaactggtt ttagagacct tgacaagatt acaacaggtt
     6001 tacacccaga tcaattagtt attttagctg ctcggccagc agtggggaag actgcctttg
     6061 ttcttaatat tgcgcaaaat gtggggacta agcaaaaaaa gactgttgct attttttctt
     6121 tggaaatggg tgctgaaagt ttagtagatc gtatgcttgc agcagaagga atggttgatt
     6181 cgcacagttt aagaacaggg caactcacag atcaggattg gaataatgta acaattgctc
     6241 agggagcttt ggcagaagca ccgatttata ttgacgatac gcccgggatt aaaattactg
     6301 aaatccgcgc aagatcacgg aaattgtctc aagaagtgga tggtggttta ggtcttatcg
     6361 taattgacta cttacagttg attacaggaa ctaaacccga aaatcgtcag caagaggttt
     6421 cagatatttc aagacagctt aaaatcctag ctaaagaatt gaaagtacca gttattgccc
     6481 taagtcagct ttctcgtggc gttgagcaaa ggcaagataa acgaccagtt ttatcagata
     6541 ttcgtgaatc aggatctatt gagcaggatg ccgatattgt agccttctta taccgggacg
     6601 attattaccg taaagaaggt gatgatgctg aagaagctgt tgaagataac acaattgaag
     6661 ttatcctcga gaaaaataga gctggggcgc gtggaacagt caaactgatg tttcaaaaag
     6721 aatacaacaa attctcaagt atagcccagt ttgaagaaag ataagaaaga gagacaaaaa
     6781 atgagcgatg catttacaga tgttgcaaaa atgaaaaaaa ttaaagagga cataagagcc
     6841 catgaaggac agttagtaga attaaccctt gagaatggac ggaaacggga aaaaaataaa
     6901 attggtaggc ttattgaggt ttacagttcg ctctttatca ttgagtatag tgatagctct
     6961 gatactccag gagcaattga taattcttat gtcgaatcat acacctattc agatattttg
     7021 acagaaaaaa cattaattcg ttatttagat tgatttttgc ctcatagagg ctttttcttt
     7081 acaataaatt gatataatta ttagcgatag aagattattc tgcctgtatt ctttttattt
     7141 tgaaaaaatt cctataaaag ataatatttt taggttagat tgaaggaaaa aagaggtctt
     7201 caccatggtt aagcaaatga tcttagtact aaggttaaaa gattatcgtg aaataaagtt
     7261 ttgacacaag tgtaattaat agaggaactt gaagtatccg gtaaaactat cttgaagtga
     7321 aaaaacgagt agacctcccc tgataaatac aatatactag ccatttcaga gcaattaaga
     7381 gtttccagtg aagtgctttt gatagagagc taagaaaaaa acagtcaagg tttaagaact
     7441 gcaaattagc atttaaatta ttttttgtta atgacagttg tctttattac cataactata
     7501 acttcttata atatagtctt cttaattcat cagtggtggt tcgtcccata tctgacctta
     7561 aaatattaaa ctgatcatca gatgatagat agactgactt tacaaaacaa ctttcttatg
     7621 gtaaactgtt ctttgtgcga aataacagca gaaaaatatg aagctcgtca acaggtggtt
     7681 atctattcaa gtaacctttg ctgtttaggc gaaaaccccc tgcacgaatc aggatttttt
     7741 aaaccgttat ttcctcaaaa aatttagagg aggacatgta atatgtcacg ttatactggt
     7801 ccatcatgga aacaatcacg tcgccttggt ttatcactta caggcacagg taaagaattg
     7861 gcacgtcgta actacgttcc tggtcaacac ggtccaaaca accgtagcaa actctcagaa
     7921 tatggtttgc agttagctga gaaacaaaaa cttcgtttct catacggttt aggtgaaaaa
     7981 caattccgta acttgttcgt tcaagctaca aaaatcaaag aaggaactct tggtttcaac
     8041 tttatggttc ttttagaacg tcgtcttgac aacgttgttt accgtctagg tttagcaact
     8101 actcgtcgtc aagcacgtca attcgttaac cacggtcaca tccttgttga cggtaaacgc
     8161 gttgatatcc cttcataccg tgttgatcca ggtcaagtga tttcagttcg cgaaaaatca
     8221 atgaaagtac ctgcaatcct tgaagctgtt gaagctactc ttggacgtcc agctttcgta
     8281 tcatttgatg ctgaaaaact tgaaggttca ttgactcgcc ttccagaacg cgatgaaatt
     8341 aacccagaaa tcaatgaagc acttgtcgtt gaattctata acaaaatgct ttaattttaa
     8401 gattaaactt acaaaaa
//
LOCUS       DAUDYX010000065         8330 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.066, whole genome shotgun sequence.
ACCESSION   DAUDYX010000065 DAUDYX010000000
VERSION     DAUDYX010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.066"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..1255
                     /locus_tag="VHD02_001680"
     CDS             <1..1255
                     /locus_tag="VHD02_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEQ5498270.1"
                     /translation="DEDLYYVKLENGLTVYFIKKIGFLEKTAMLTVGFGSLDNKLTVD
                     DESRDAPAGIAHFLEHKLFEDESGGDISLKFTQLGAETNAFTTFNQTSFFFSTASKFQ
                     ENLELLQYFVLSANITDESVSREKKIIGQEIDMYQDDADYRAYSGILQNLFPKTSLAN
                     DIAGSKASIQKITKILLETHHTYFYQPTNMSLFIVGDIDIDETFLAIQRFQTTLSYPD
                     RKRVTVDPLHYYPVIKSSSVDMDVTTAKLVVGFRGYLTLTQHSLLTYRIALKLFLSML
                     IGWTSKIYHTLYEDGKIDDSFDVDVEIHHHFQFVLISLDTPEPIAMSNYIRQKLATIK
                     ISKEFTNEHLNLLKKEMYGDFIQSLDSIEHLTHQFSLYLSDSDKETYFDIPKIIERLT
                     LKDVVTIGKAFFEKADASDFTVFPK"
     gene            1305..2330
                     /locus_tag="VHD02_001681"
     CDS             1305..2330
                     /locus_tag="VHD02_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ5498271.1"
                     /translation="MSEKSLGEVLRESRIGKNITLDDIELKTGISSHYLLAMELDQFK
                     IIPEEKFDQFLKEYADIVELDFNTLKRRYRYQVNSKKDSDIKSVTEIVEEKLSKKRLQ
                     EKLLSHQSMGYGQPLEVSKNKETLVIPVKTVGLDSKSPLKTISPVSQFKSDNRLIPKK
                     NVSRMRRNSTDSRSKTSLFPIAILGTVAFAIISVIILGVWQQFEKSQRAKEAEVALLK
                     ASEQSTNGLKNNSNDRKTQVTTEGSGNYLIATVTKSKETVDISVSLTEAQSSWISLTN
                     SEIGEGGITLTQDSPTYTATLPAEITESLLTLGVTKGVSVTIDGQPVDLSPLTSTDLS
                     YITFKIQ"
     gene            2344..2886
                     /gene="pgsA"
                     /locus_tag="VHD02_001682"
     CDS             2344..2886
                     /gene="pgsA"
                     /locus_tag="VHD02_001682"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEQ5498272.1"
                     /translation="MIKKENIPNLLTLVRIAMIPFFLFITSSSNKVGWHIFAAVIFAI
                     ASFTDYLDGYLARKWHVASNFGKFADPLADKMLVMSAFIMLVGLGLVPAWVSAVIICR
                     ELAVTGLRLLLVETGGKVLAAAMPGKIKTATQMLSIILLLCHWIFLGNVLLYIALFFT
                     IYSGYDYFKGASFLFKDTFK"
     gene            2888..3727
                     /locus_tag="VHD02_001683"
     CDS             2888..3727
                     /locus_tag="VHD02_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEQ5498273.1"
                     /translation="MSAIIELKKVTFNYHKDQEKPTLDGVSFHVKQGEWLSIIGHNGS
                     GKSTTIRLIDGLLEPESGSVIVDGDLLTITNVWEIRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIAHEDIKERVNHALELVGMQNFKEKEPARLSGGQKQRVAIAGAVAMKPK
                     IIILDEATSMLDPKGRLELIKTIKNIRDDYQLTVISITHDLDEVALSDRVLVMKDGQV
                     ESTSTPEQLFARGDELLQLGLDIPFTTSVVQMLQEEGYPVDYGYLTEKELENQLCQLI
                     SKM"
     gene            3703..4545
                     /locus_tag="VHD02_001684"
     CDS             3703..4545
                     /locus_tag="VHD02_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEQ5498274.1"
                     /translation="MSINLQNVSYTYQAGTPFEGRALFNINLDILDGSYTAFIGHTGS
                     GKSTIMQLLNGLHVPTTGIVSVDKQDITNHSKNKEIKSIRKHVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGVSPEEAEALAREKLALVGISENLFEKNPFELSGGQMRRVAIAGIL
                     AMQPKVLVLDEPTAGLDPKGRKELMTIFKKLHQSGMTIVLVTHLMDDVANYADFVYVL
                     DKGKIILSGKPKTIFQQVSLLEKKQLGVPKVTKLAQRLVDRGIPISSLPITLEELREV
                     LKHG"
     gene            4538..5338
                     /locus_tag="VHD02_001685"
     CDS             4538..5338
                     /locus_tag="VHD02_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEQ5498275.1"
                     /translation="MDKLILGRYIPGDSLIHRLDPRSKLLAMIIYIVIIFWANNVVTN
                     LLMLTFTLAVVFLSKIKLSFFLNGVKPMIGIILFTTLFQMFFSQGGKVIFSWWFISIT
                     DLGLSQAILIFMRFVLIIFFSTLLTLTTTPLSLSDAVESLLKPLTRFKVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIQKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGEGRTKYRQLDWQLKDSLAIGIVSLLGLLLFFLKTSL"
     gene            5710..6324
                     /locus_tag="VHD02_001686"
     CDS             5710..6324
                     /locus_tag="VHD02_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEQ5498276.1"
                     /translation="MFKKENLKQRYFNFGLVALALTILAIIFAFSSKNADTKSYAKKS
                     ESKMVTIDKAPKNNHAITKEESKEKAKSIASEPIPTVENSVAPTVTEEAPVVQQEVTQ
                     TVQQVSSVAYNPNNVVLSNGNTAGIVGSQAAAQMAAATGVPQSTWEHIIARESNGNPN
                     AANASGASGLFQTMPGWGSTATVEDQVNAALKAYSAQGLSAWGY"
     gene            complement(6738..7610)
                     /gene="sdaAA"
                     /locus_tag="VHD02_001687"
     CDS             complement(6738..7610)
                     /gene="sdaAA"
                     /locus_tag="VHD02_001687"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEQ5498277.1"
                     /translation="MFYTIEELVKQADQQFNGNIAELMIATEVEMSGRNREDIIKIMS
                     RNLQVMKAAVTEGLTSTKSISGLTGGDAVKMDNYIKKGNSLSDTTILNAVRNAIAVNE
                     LNAKMGLVCATPTAGSAGCLPAVLATAIEKLDLSEKKQLEFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSAAAMSAAALVKAAGGTSHQASQAIAFVIKNLLGLVCDPVAGLVEV
                     PCVKRNALGASFALVAADMALADIESQIPVDEVIDAMYQVGSAMPTAFRETAEGGLAA
                     TPTGRRYSVEIFGE"
     gene            complement(7622..8293)
                     /gene="sdaAB"
                     /locus_tag="VHD02_001688"
     CDS             complement(7622..8293)
                     /gene="sdaAB"
                     /locus_tag="VHD02_001688"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEQ5498278.1"
                     /translation="MNTQKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVYSIFGDIPD
                     EVTFHLYNSFAKTYRGHGTDKALVAGIMGMDTDNPDIKNSLEIAHQKGIKIYWDILKD
                     SNAPHPNTVKISVKKADKTLSVTGVSIGGGNIQVTELNGFSVSLSMNTPTIVTVHKDI
                     PGMIAKVTDILSSNNINIATMNVTRESAGEKATMIIEVDSRECQEAANQIAKIPHIYN
                     VNFFD"
BASE COUNT         2683 a         1394 c         1541 g         2712 t
ORIGIN      
        1 tgacgaagac ctttactatg taaaattaga aaatggttta actgtttatt ttataaaaaa
       61 aatagggttt ttagaaaaaa cagctatgtt gacagtaggt ttcggttctc ttgacaataa
      121 gttgacagtc gatgacgaat cacgagatgc tccagcaggt attgcacact ttttagaaca
      181 taaactcttt gaagatgaat caggtggaga tatttcatta aagtttacac agctaggagc
      241 agagacaaat gcctttacaa ctttcaacca aacgagtttc ttcttttcaa cagctagtaa
      301 atttcaggaa aatttggaac tccttcaata ttttgttctt tctgctaata taacagatga
      361 atctgttagt agagaaaaaa aaataatcgg gcaagaaatt gatatgtatc aagatgatgc
      421 tgactaccgt gcatatagtg gaatccttca gaacttattt ccaaaaacaa gtttagctaa
      481 tgatattgct ggctcaaaag catccattca aaagatcacg aaaatattat tggaaacgca
      541 tcatacttat ttttaccaac ctactaatat gagtctattc attgtcggtg atattgatat
      601 tgacgaaact tttttagcta ttcaaaggtt tcaaaccaca ttatcctatc cagatagaaa
      661 aagggtaact gttgatcctt tacactacta tcctgtcatt aaatcatctt ctgttgacat
      721 ggatgttact actgccaagc tggttgttgg ttttcgaggg tatctgacct taactcagca
      781 ttcactatta acgtatcgta ttgccttgaa gctattttta tcaatgctta taggttggac
      841 ttctaaaatc tatcatacgc tatatgaaga tggtaaaata gacgattcat ttgatgtgga
      901 tgttgaaatt catcatcatt ttcaatttgt attaatatca cttgatacac cagagcctat
      961 cgcaatgtct aactatatcc gtcaaaaatt agccactata aagatatcaa aagagtttac
     1021 aaatgagcac ttaaatctat taaaaaaaga aatgtatggt gactttattc agagtttaga
     1081 ctctatagaa catttaaccc atcagttcag tctatacctg tctgattctg ataaagaaac
     1141 atattttgat attccaaaaa ttatagaaag actcactcta aaagatgttg ttacaattgg
     1201 aaaagcattt tttgaaaaag ctgatgcttc agacttcaca gtcttcccaa aatagatgaa
     1261 aatctgttat aatagggtta ctatattatt tttgaggtac ctgtatgagt gaaaaaagtc
     1321 ttggtgaagt gttaagagaa tcgcgtattg gaaaaaatat cacactagat gatatagaat
     1381 tgaagacagg gatttcttcc cattacttgc tggcaatgga attggatcag tttaaaatta
     1441 ttccggaaga aaaatttgat caatttttaa aagaatatgc tgacattgtt gagttagatt
     1501 tcaatacttt aaaaagacga taccgttatc aagttaattc caaaaaagac agtgatataa
     1561 aatctgtaac tgagattgtt gaagagaagt taagtaagaa acgattacaa gagaagttgt
     1621 tatcacatca gagcatgggg tatggtcaac cgttagaagt ttctaaaaac aaagaaacct
     1681 tagttatccc tgtaaagact gttggtttag atagcaaatc gccactaaaa acgatatcgc
     1741 ctgttagcca atttaaatca gataacaggt taattcctaa aaaaaatgtg tcacgtatga
     1801 ggcgaaatag cactgacagt cgttctaaaa cgtcactatt tcccatagct atattaggaa
     1861 cggttgcttt tgcaattatc agtgttatca ttttaggtgt ttggcaacaa ttcgaaaaga
     1921 gtcaaagagc taaagaagct gaagttgctt tgcttaaagc ctctgagcag tctactaacg
     1981 gtttaaaaaa taattcaaat gatcgcaaaa ctcaagtcac aactgagggg tcgggtaatt
     2041 atttaatagc gactgttact aaatctaaag agaccgttga tatcagtgtg tctttaactg
     2101 aggcacaaag tagttggatt tctctaacaa attcagaaat tggtgaaggt ggtataacat
     2161 tgactcaaga ctcgccaact tatacagcga ctttacctgc agaaataaca gaatcattgt
     2221 tgacacttgg tgtgacaaaa ggtgtatctg taacaattga tggacaacct gttgatctat
     2281 cacctttaac aagtactgac ttaagttata tcacgtttaa gatccaataa ttaaggtaga
     2341 ctaatgataa aaaaagaaaa tattcctaat ctattaactc ttgttcgaat cgcaatgatt
     2401 cctttctttc tttttattac ttcctcatct aataaggtgg ggtggcatat ttttgcagct
     2461 gtgatttttg caattgccag ttttaccgat tatttagatg ggtatcttgc gcgtaagtgg
     2521 catgtagcca gtaattttgg aaagtttgcc gatcctctag cagataagat gcttgtcatg
     2581 agtgccttta tcatgttagt tggacttggc ttagttcctg cgtgggtgtc agctgttatt
     2641 atttgccgag aattggcagt aactggtctt cgattactac ttgttgaaac cggaggaaag
     2701 gtccttgcag ctgctatgcc gggaaaaatc aaaacagcga cacaaatgtt gtctattatt
     2761 ttattacttt gccattggat attcctagga aacgtcttac tctatattgc tctttttttc
     2821 actatttatt ctggatatga ttattttaaa ggagcaagct ttctttttaa ggatacgttt
     2881 aaataacatg tcagctatta ttgaacttaa aaaagttaca tttaattacc ataaagacca
     2941 agaaaaacca acattagatg gcgtatcgtt tcatgtgaaa caaggtgagt ggctgtctat
     3001 cattggccat aacggatctg gtaaatcaac gactattcgg ttaattgatg gattattgga
     3061 accagaatca gggtctgtta ttgttgatgg agatctacta accataacta atgtttggga
     3121 aattcgtcat aaaattggta tggtctttca aaaccctgat aatcagtttg ttggagcaac
     3181 tgttgaagat gatgttgctt ttgggcttga aaataaaggt attgcacatg aagatataaa
     3241 agagagggtt aatcatgctt tagagttagt cggcatgcag aactttaaag aaaaagaacc
     3301 agcccgttta tctggtggcc aaaaacagcg cgtagctatt gcaggtgcag ttgctatgaa
     3361 gcctaaaatt attattttag atgaagctac tagtatgctt gaccctaaag gacgattaga
     3421 gttaatcaaa actataaaaa acatccgtga cgactaccag ctgactgtta tttccattac
     3481 tcatgactta gatgaagttg ctcttagtga tagagtttta gtgatgaaag atggtcaagt
     3541 ggaatcaaca tcaacaccag aacaattatt tgcaagaggg gatgaactac tccaattagg
     3601 tcttgatatc ccttttacaa catctgttgt acagatgctt caagaagaag gttatcctgt
     3661 tgactatgga tatctcacag aaaaggaatt agaaaatcag ttatgtcaat taatctccaa
     3721 aatgtaagtt atacttatca agcagggact ccttttgaag ggcgtgccct ttttaacatt
     3781 aatttagata ttttagatgg ttcttatacg gcttttatcg gacatactgg atccgggaaa
     3841 tcgaccatta tgcaattgct aaatggttta cacgttccga caacgggaat tgtcagcgta
     3901 gataaacaag atataacgaa tcactctaaa aataaagaga ttaaatctat tcgaaaacat
     3961 gtaggattag tttttcaatt tccagaaagt caactatttg aagagacagt tttgaaagat
     4021 gttgcttttg gccctcaaaa ttttggggtc tctccggagg aagcagaagc tttagcgcgt
     4081 gaaaaactag ctcttgttgg gatttcagaa aatctttttg aaaagaatcc atttgaactc
     4141 tcagggggtc aaatgcgtcg tgttgctatt gcgggtatcc ttgctatgca gccaaaggtt
     4201 ttagttttag atgaaccgac tgctggtctt gatcctaaag ggcgtaaaga attaatgact
     4261 atttttaaaa agttacacca atctggcatg actattgttt tagtaaccca tctgatggat
     4321 gatgtggcca attacgcaga ttttgtatat gttcttgaca aaggtaaaat tattttatca
     4381 ggaaaaccca aaacaatttt tcaacaagtc agtttgcttg agaaaaaaca gttaggagtt
     4441 cctaaggtga ctaaacttgc tcaaaggctt gttgatagag gaatacctat ctcttctttg
     4501 cctattacct tagaggagct aagagaggta ctaaaacatg gataagctga ttcttggtcg
     4561 ttatattccc ggagattctc tcattcatcg tcttgaccca agaagtaaat tattagcgat
     4621 gattatttat attgtcatta tcttttgggc taacaatgtt gtgacaaatt tgttgatgtt
     4681 gacttttact ttagcagttg tttttttgtc aaaaatcaag ttgtctttct ttttaaatgg
     4741 ggttaaacca atgattggta ttatcctctt cacgacttta tttcagatgt ttttctcaca
     4801 aggaggaaaa gtaattttta gttggtggtt cattagcata actgatttag gattgagcca
     4861 agctatttta atttttatgc gtttcgttct aattattttc ttttcaacgc tgttaacgtt
     4921 gacgacaaca cctttaagcc tatctgatgc agttgaatca ttattaaaac ctttaacaag
     4981 atttaaagtt ccagctcacg agatcggact aatgctttct ttaagtttac gttttgtgcc
     5041 tactttaatg gatgatacca cgcgtattat gaatgcacaa agggcccgtg gagttgattt
     5101 tggagaggga aatttaattc aaaaagttaa atccattatt cctattttaa tccctctttt
     5161 cgcttcaagc tttaaaagag ccgacgcatt agctattgct atggaagcta gaggttatca
     5221 gggaggagaa gggcgaacta aatatcgtca attagactgg cagctaaaag atagtttagc
     5281 tattggaata gtttctttat tgggtttgct actttttttc ttgaaaacct ctttgtaata
     5341 ttttttagca taaaactttt ctttattagt aagaagtgaa ttgtacctca aaagttagat
     5401 acaacatcta atcattgagg tacttttctt tatttgagga attgaaaacc gctatctcaa
     5461 ctatattaat catcccaaca ataacaaatc gaacgaaatc taaaaggact tagtcctgtt
     5521 caatacagaa ctaaatcctt tgtttaatga tattatcccg ctttttaatg ttgctaagaa
     5581 gaagagtttt atgatttttt acaacttctt ataatatcaa tgtagttgta atatttttaa
     5641 tatatcttca ataacgaggt aataattaaa agatattatg gataacggag aaaagtgagg
     5701 tattattgaa tgtttaagaa agaaaattta aaacaacgtt attttaattt tggattagta
     5761 gcgttagctc taacaatatt agccatcatt tttgccttct caagtaaaaa tgctgatact
     5821 aagtcttatg ctaagaagtc agaaagtaaa atggtaacaa tcgacaaggc tccaaaaaat
     5881 aatcatgcta ttactaaaga agaaagcaaa gaaaaagcaa agagcattgc ttcggagcct
     5941 attcccacag tagaaaactc tgtagctccg acagtaacag aggaagcacc ggttgttcag
     6001 caagaagtga ctcaaactgt tcagcaggta tcttcagtag cctataatcc aaacaatgtg
     6061 gtactttcca atggaaatac tgctggtatt gtaggaagtc aagcggcggc acagatggca
     6121 gcagcaacag gtgttccaca atcaacttgg gaacatataa ttgcgcgtga atctaatgga
     6181 aatcctaacg cagctaatgc ttctggggca tcagggttgt tccagacaat gccaggttgg
     6241 ggttctacag caacggttga agatcaagtc aatgcagcct tgaaagccta tagtgcacaa
     6301 ggtttatcag cttggggtta ctaaataaac atggctggaa gaaataaaac ctattgttag
     6361 tatttactaa caataggttt ttggtattta attgtcttta tgatttgtag tgatgaatta
     6421 gtctcaaaac aaaacattgc cttatcaggg aatatagctc gtattattta ggaaaactga
     6481 ccagcactta agtgcctctg atatttaagg cagttatact gataacaagc aggaaaaaaa
     6541 ctcttaaatg tttataaggt cattagcaat tttcagtatt ttacactact acacttttga
     6601 gttttaacaa tttactagaa gttgtttcta aggggaaaca acatagcttg atagctatct
     6661 attagtcatt ttagtgaata tatagcataa aaataaaacg ctttcaaata tttctaagaa
     6721 tttttctaac tatctattta ttcgccaaat atttctacgc tgtaacgtcg tccagttgga
     6781 gtagcggcta gaccaccttc tgctgtttca cggaaagcag ttggcatggc agagccgact
     6841 tgatacatag catcaataac ttcgtcaaca ggaatttgag attcaatatc agctaaagcc
     6901 atatcagctg cgacaagggc gaaacttgct cctagggcat tgcgtttgac acaaggcact
     6961 tctactaggc cggcaacagg atcacagaca agtcctaata aatttttgat aacaaacgca
     7021 atagcttgac tagcttgatg agatgttcca ccagctgctt taacaagagc agctgcactc
     7081 atagcagctg cagaaccaac ttctgcttgg catccacctt ctgcaccaga aatagaggcg
     7141 ttatttccaa taacaagacc aaaagctcct gcagtaaata aaaactctaa ttgttttttt
     7201 tctgataagt caagtttttc aatagctgtt gcgagcactg ctggtaagca tcctgcactt
     7261 cctgcagtgg gtgttgcaca gacaagtccc attttagcat ttaattcgtt tacagctata
     7321 gcatttctaa cagcattaag aatagtagta tcagaaaggc tgttcccttt tttaatgtag
     7381 ttatccattt tgaccgcatc gccccctgtc aaaccactga tagatttggt tgaggttagt
     7441 ccttctgtta cagctgcttt cataacttgt aaattgcgtg acatgatttt gataatatct
     7501 tctcgatttc ttccagacat ctccacttcc gtggcaatca tgagttcagc aatatttcca
     7561 ttaaattgtt ggtcagcttg tttgacaagt tcttcaatag tataaaacat agctttcctt
     7621 tctagtcaaa gaagttgaca ttataaatat gaggaatttt tgcaatttgg ttggctgctt
     7681 cttggcactc tctagaatcg acttctataa tcatagtagc tttttcacca gcagattcac
     7741 gtgtaacatt cattgtggcg atattaatat tattggaaga taggatatca gttacctttg
     7801 cgatcattcc gggaatatct ttgtgtacag taactatagt aggagtattc atggataacg
     7861 agacagaaaa accatttagt tctgttactt gaatgtttcc tcctccaatt gaaacaccag
     7921 taacactaag tgttttgtca gcttttttga cactgatttt aacagtattg ggatggggag
     7981 cattgctatc ttttaagata tcccaataga tttttatccc tttttgatga gcaatttcta
     8041 gagaattttt aatatcagga ttatcagtat ccatacccat aattcctgca acaagagcct
     8101 tatcagtacc atgtcctctg taggttttag caaatgaatt ataaaggtga aacgtcactt
     8161 catcaggaat atctccaaag atggaataga ctaccttacc aattctgacg gcaccagctg
     8221 tgtgactact tgatggacca atcattactg gcccaatgat gtcaaaaact gattgaaatt
     8281 tttgtgtatt cataggcaac tccgtttttt atttctatgt ttattataac
//
LOCUS       DAUDYX010000066         8279 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.067, whole genome shotgun sequence.
ACCESSION   DAUDYX010000066 DAUDYX010000000
VERSION     DAUDYX010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8279
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.067"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(<1..312)
                     /locus_tag="VHD02_001689"
     CDS             complement(<1..312)
                     /locus_tag="VHD02_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HEQ5498279.1"
                     /translation="MILGDEDALAKIAISYQQNTKKEDAVCEKHGCRYITVLKTGLTV
                     CPDCHREELDNQNALHVQKQYERELENKRLYYLKKLSIMDDELENASFDNFRADTAKH
                     KE"
     gene            complement(299..1129)
                     /locus_tag="VHD02_001690"
     CDS             complement(299..1129)
                     /locus_tag="VHD02_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEQ5498280.1"
                     /translation="MADNKKYYYLKLKENFFESDEAIILESMPDGYIYSNILLKLYLR
                     SLKNDGLLMFNNLIPYNAQMLATITRHQIGTVEKAIQIFRDLQLIEILDNGAIYMTNI
                     QNFVGKSSTDADRKRIEYAKAKQLGQISMKCAEKSPPEIEIELDKERELDKKIDINID
                     KELEVEEEIKDSSSAANVNNLKIISDYFQQEIGILSPNQFEQLSDYITITKMEVDVVK
                     EAITRAADNSKRSFGYVNSILRNWRQNGILTMVKVEEEQRQFQSRKSKQEEVSEYDTW
                     "
     gene            1343..1804
                     /locus_tag="VHD02_001691"
     CDS             1343..1804
                     /locus_tag="VHD02_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498281.1"
                     /translation="MIALVHSIYYNMVKIKLSDCYISRVDKGYDWMYDFSISNLSNVS
                     VIIKKIELYNKDGKLISDNGFNPFQKYEADMQNEADDYYGLSMPNYYMPLDYQWESSP
                     FKSDTEVYPSSRENFSYYLDEKPVKIKITTDKRIHQFRKYQLFFPHFDNNS"
     gene            1935..2144
                     /locus_tag="VHD02_001692"
     CDS             1935..2144
                     /locus_tag="VHD02_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498282.1"
                     /translation="MGPNYFHVQFKLGEKVSYNTPSAEGREVIPIKGAEVTKMIFADG
                     NELLSVIHNETVDVYASFPIVLEYH"
     gene            complement(2254..2454)
                     /locus_tag="VHD02_001693"
     CDS             complement(2254..2454)
                     /locus_tag="VHD02_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ5498283.1"
                     /translation="MTRFAIQRLREEKKMSQEELANKSGVSRTTISLIETDKSTTVKL
                     STLQKLAVALDVPIGYFFKHNV"
     gene            2528..2914
                     /locus_tag="VHD02_001694"
     CDS             2528..2914
                     /locus_tag="VHD02_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498284.1"
                     /translation="MFDYFKLYIIVLETFVETKPDDAYSLFDNLSLNSEFVKFSKDLD
                     KNIVISGTLEVIDNLLDDCLIKGKRRPTKDITFYFFDGVTTTGYLYLQSLKDNNFSSR
                     LKNVLKEEGIPLTPTSITRTIAKLTL"
     gene            complement(2903..3112)
                     /locus_tag="VHD02_001695"
     CDS             complement(2903..3112)
                     /locus_tag="VHD02_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498285.1"
                     /translation="MRPKKYPYSQKNYPAPKLHNVIETDNQFLIDDKRIHYVIEDSVK
                     TKTLGDGYVEVTLSIIAKSFTKSQG"
     gene            3166..3765
                     /locus_tag="VHD02_001696"
     CDS             3166..3765
                     /locus_tag="VHD02_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498286.1"
                     /translation="MDLLKNTNFLIPLISAIVSVSSIFISNWLGYRSQIRKLKFDEEK
                     EIYLTLYVPLIKWMNSQSFNNKSYYWLVAFPRYTTNAQDFLTGLLLKNFEKLPVSVAM
                     RYSEYTLNSATSLHFYRNTEYDYDYETFAKKASELFDLIIEQLLTEGTILSQKLSLPN
                     LSKSTLENFLADKKNYIGPRFLSLETHNKPLRPERPLPF"
     gene            complement(3795..3953)
                     /locus_tag="VHD02_001697"
     CDS             complement(3795..3953)
                     /locus_tag="VHD02_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498287.1"
                     /translation="MNWKKLMLGNLEHTFTSHDGKEKTSIEFEGGLLPALLVLGGITW
                     LAAWFITK"
     gene            4310..5134
                     /locus_tag="VHD02_001698"
     CDS             4310..5134
                     /locus_tag="VHD02_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ5498288.1"
                     /translation="MKLGEIIKNFREEKKLSMDRFAEKSGLTKGYISMLEKNEHPKSK
                     KPIIPTEETLLKVAKGMGVDIDFVLSKLDSDQEIQINISPKNMLNMDNPSTPTTPKVE
                     LIPSTLQKINSTSSQLEHSRQIIVLDTAETLLEQQKEIKNNEDTIAELFSYNYYDHAA
                     SAGTGQYLNDVQVEKIELPVDYDADFVIPVYGDSMEPKYHSGDYVFVKLSVELTDGDI
                     GVFEYYGDAYIKQLLINDEGAFLHSLNSKYEDIPIDRDSDFRIIGEVVGSYSGNHS"
     gene            5170..6063
                     /locus_tag="VHD02_001699"
     CDS             5170..6063
                     /locus_tag="VHD02_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P63C domain-containing protein"
                     /protein_id="HEQ5498289.1"
                     /translation="MENEVLHATHEGKLVLDNTTLDVAVLNNGQRIITQAGVFKALDR
                     PSRGNTRVIGIPTFMDAKNLQPLISKDLRGVINKIEYRDLNGRIQLGFDANILPLVSD
                     LYLKAREIGAIKSEAQMRTAQKAEMLVRSLAKVGVTALVDEATGYQYERERFELQKIF
                     KAYISEELLKWQKMFPDDFYMEIFRLNGWSYDVQSIKRRPGVVGKWTNELIYKRLPDG
                     VLEELKRRTPKTKKGQYKARFFQTLTPDIGHPDLNAQIYKVLGIMRISSNWKDFKEKF
                     NVMTSREDGIIELDLEFDEDK"
     gene            6184..7272
                     /locus_tag="VHD02_001700"
     CDS             6184..7272
                     /locus_tag="VHD02_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEQ5498290.1"
                     /translation="MKIKSYKKENGETAYKFLLYAGYVNGKRKYIRREGFKTKQAARE
                     TLISLQAELDKPKSSMTFGALTDQWLKEYEKTVQCSTYLKTERNINKHILPKLDKVKI
                     GDINPLLIQRLTEEWCNDLKYGGKILGLVRNILNLAVRYGYINNNPALPITPPKIKRR
                     RKMNNNFYTLDQLKQFLELVEKTDNIEKIALFRLLAFTGIRKGELLALTWDDLNGNTL
                     SINKAVTRTQVGLEIDVTKTKSSDRLISLDDETLEILLELHETFPTSTLMFQSESGGI
                     MTPSLPRKWLLQIIKGTDLPQITIHGFRHTHASLLFESGLSLKQVQHRLGHGDLQTTM
                     NVYTHITQSAIDDIGTKFNQFVTNKQLD"
     gene            7635..8255
                     /locus_tag="VHD02_001701"
     CDS             7635..8255
                     /locus_tag="VHD02_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEQ5498291.1"
                     /translation="MTKKHLLTLLLISFFTSFLVACSTTKDKEPQPSDSEIITPRLHQ
                     AAHQDKRANFEKIKLATVDSSFTGGTSLEELISLFGEPSQHDPKTAGEVTIDAYTWQF
                     DQVTLTVNLYQNSSIVKTISNFTFARELGLSQKEYQQLQKGMSYEDVKKILTEPDNYS
                     QASSSDHQTLQAIWVSGLKTDTSGANISLVFENNQLTEMSQVGLEE"
BASE COUNT         2758 a         1453 c         1374 g         2694 t
ORIGIN      
        1 ctctttgtgt ttagccgtat cagccctaaa attgtcaaat gatgcatttt ccaactcgtc
       61 gtccataatt gataattttt tgagatagta cagtcgcttg ttttcaagct ctctctcata
      121 ttgcttttgg acgtgcaaag cgttttgatt gtctaattct tctctgtgac aatctggaca
      181 tactgtcaaa ccagtcttga ggactgtgat gtatctacaa ccatgttttt cacagacagc
      241 atcttctttc ttggtgtttt gttgataaga tatagcgatt ttagcaagtg cgtcttcgtc
      301 accaagtatc atattccgat acctcttctt gtttagattt tctagattgg aattgccttt
      361 gttcttcttc aactttaacc atcgtaagga ttccgttttg tctccaattt cgcaatatag
      421 agttaacgta tccaaaagaa cgtttagaat tgtcagcagc tctcgttatc gcttccttaa
      481 caacatccac ttccattttt gtgatggtga tatagtctga caactgttca aactgattcg
      541 gagataagat gccaatttct tgttgaaaat aatcagatat aatttttaag ttatttacat
      601 tagcagcaga agatgaatct tttatctctt cttctacttc taactcttta tctatattta
      661 tatctatctt tttatctaac tctctctctt tatctaactc tatctctatc tctggtggag
      721 atttctccgc acatttcatg gagatttgtc caagttgttt agcctttgca tattcaattc
      781 tttttctgtc agcatctgtg cttgatttac caacaaaatt ttgaatattt gtcatatata
      841 tcgcaccgtt atcgagaatt tcgattaact gtaggtctct aaaaatttga atagcctttt
      901 cgacagtgcc aatctgatgc cttgtaattg tggcaagcat ttgtgcgttg taaggaataa
      961 gattgttaaa cattaataag ccatcatttt ttaaacttct tagatataac ttgagcaaaa
     1021 tattgctata gatatagccg tcaggcatac tttctaaaat gattgcttca tcactttcaa
     1081 agaaattttc ctttaatttt aagtaataat actttttgtt atctgccatt tatctccctt
     1141 cttcaaaaat agctttcaca tttcttatct tttaacgtct caataattcc atctaaaaca
     1201 tctgatcggt ttttaagctc attgtactct ttgacagata ttgtgataaa atctttgtta
     1261 tcttttgtag tatcgctata tccgagcagg taagcgaccg atacatcaaa atgatctgct
     1321 agtaattgcg ctaattgttg ctgtgattgc tcttgttcac tctatctact acaacatggt
     1381 taagataaaa ttatctgatt gttacatttc aagagtagat aaaggttacg attggatgta
     1441 tgattttagt atcagtaact tatcgaatgt ttcagtcatt attaaaaaaa ttgaacttta
     1501 caacaaagat ggaaaactaa taagcgataa tggcttcaat ccctttcaaa aatacgaagc
     1561 tgacatgcag aatgaagctg atgattatta tggattgtca atgccgaatt attatatgcc
     1621 attagattac caatgggagt catcgccatt taagtcagat actgaagtat atccatctag
     1681 tcgagaaaat ttctcttact atttagatga aaagccagtt aaaatcaaaa tcacaactga
     1741 taagcgtatt catcaattcc gaaaatatca gttattcttt ccccattttg acaataatag
     1801 ttaagatagc gatattcgtt aataaaatta agataagtgc tgccgttagt agcatttttt
     1861 cttttctcct tttattagtt ttgttcctcc tgcgtgctat aataaagcta tcattacgaa
     1921 aggaggaaaa aagcatgggt cctaattatt ttcatgtcca atttaagtta ggcgaaaaag
     1981 tatcgtacaa cacgccttca gcagaaggaa gagaagtcat ccctatcaaa ggtgctgaag
     2041 ttacgaaaat gattttcgcc gatggtaacg aattgttaag cgttattcac aacgaaaccg
     2101 ttgacgttta cgctagcttc ccaattgttc ttgagtatca ttaattcgtt atttctaaac
     2161 cagcaatcgc ccgctactgc gtttgttggt ttttcttttc cgtaaaaaat tcgatttgct
     2221 tgcatagtgt cctttccagt tttgtttaac acgttaaaca ttatgtttaa aaaaatatcc
     2281 aataggtacg tctaaagcga ctgccaattt ttgaagagtg cttagcttaa ccgtagtaga
     2341 tttatcggtt tcaataagag atatagtagt tcgtgagact ccagatttat tggctagctc
     2401 ttcttgagac attttctttt cttcacgcaa tctttgaatt gcaaacctcg tcattccccc
     2461 acctcctttc tatctgtttt tagttcctcc aatctgctat aatatgggca gaaaggaggt
     2521 gaatgtgatg tttgactatt tcaaacttta tataattgtc ttggaaacct tcgtggaaac
     2581 caaaccagac gacgcttata gcttatttga taatctaagt cttaacagtg aatttgtaaa
     2641 attctctaaa gacttagata aaaacattgt aatatcaggc actttagaag taattgataa
     2701 tttacttgat gattgcttga taaaaggaaa acgtcgccca acaaaagata ttactttcta
     2761 ttttttcgat ggcgtaacaa caactggtta cttatatctt caaagtctta aagataataa
     2821 tttttcgagc cgtttaaaga atgtccttaa agaagaaggt atccctctta caccaacttc
     2881 tatcacaagg actatcgcca aactaacctt gtgattttgt aaaactcttg gcaattatgg
     2941 aaagtgttac ttcaacatag ccatcgccaa gggtttttgt ctttacagaa tcttcaataa
     3001 cataatgaat ccttttatca tctattaaga actgattatc ggtttcaatg acgttatgaa
     3061 gcttcggcgc tggataattt ttttggctat acggatattt tttggggcgc atctattcca
     3121 ccccctttct gtggtataat ttaaataaaa tgattggaga aaatcatgga tttattgaaa
     3181 aataccaatt ttttaattcc gttaatttca gcaatagtct ctgtttcttc tatttttata
     3241 agcaattggc ttggttatag aagtcaaatc aggaaattaa aatttgatga agaaaaagaa
     3301 atatacctaa ccctctatgt accgttaata aagtggatga attcgcaatc attcaataac
     3361 aaaagttatt attggctagt ggcgttccct agatatacaa caaatgcaca agattttctg
     3421 acaggcttgt tgttaaaaaa ttttgaaaaa ttaccagtat cagttgctat gagatactct
     3481 gaatacaccc taaattctgc aacctcttta catttttatc gtaacaccga atacgattat
     3541 gattatgaaa catttgccaa aaaagcatct gaattatttg atctcattat tgaacaattg
     3601 ctaacagaag ggacaatatt atcccaaaag ctaagcttac caaacttatc caaatccact
     3661 ttagagaact ttttggcgga caagaaaaac tatatcggtc ccagattttt atcactagaa
     3721 acccataaca aacccctaag acctgaaaga ccactgccat tttaactccc ccctttccgc
     3781 cccttgtggg ctttttattt tgtaataaac caagctgcta accaagtgat accacctaac
     3841 accaacagcg ctggcaataa gccaccttca aattcgatgc ttgttttttc tttgccatca
     3901 tgactagtaa acgtgtgttc taagttgcca agcattagct ttttccaatt catgcaacct
     3961 ctcctttcat tcttgcggag atacagccaa tgtgctaaac taaacttacc ccgttagggg
     4021 gagaggactt cttagccctc taattatcct caccactcta ttgagtagtg aatcttaagc
     4081 ttaaaccaaa gaatcttgat ttcgacttct agttctttgc gtttaggctt tttgtttagc
     4141 ctagatttca tcagctgtac ctcctttcgt tttgcttaat cccttaagct tgattatagt
     4201 ttaacacgtt aaacattcat tgtcaagcgt gttaaacaaa aatattgaat tattttttgt
     4261 tgtgttgtat aatgtattaa acaatcattc taggaagagg tttttaacat tgaaattagg
     4321 ggaaataata aaaaatttca gggaagaaaa aaagttatca atggataggt ttgctgaaaa
     4381 atctggtctt actaaggggt atatttcaat gcttgaaaaa aacgagcatc cgaaatctaa
     4441 aaaaccaatt atccctacag aagaaacttt gttaaaagta gcaaaaggga tgggggttga
     4501 tattgatttt gttttgagta aattagattc cgatcaagaa atacagatta atatttctcc
     4561 taagaatatg ttaaatatgg ataatccctc cactcccacc accccaaaag tcgaacttat
     4621 cccatctact ctacaaaaaa taaactctac ttcttctcaa ctagaacaca gtagacagat
     4681 aattgtttta gatacagctg agactttatt ggaacaacag aaagaaatta aaaacaacga
     4741 agatactatt gccgaattat tttcttacaa ctactacgac cacgcagctt cagctggtac
     4801 aggtcagtat ctaaatgatg tacaagtaga aaaaattgag ttaccagtcg attatgacgc
     4861 agactttgtt atccctgttt atggtgattc catggaaccg aagtatcact ctggggatta
     4921 tgtatttgtt aagctatccg tagagcttac agatggcgat ataggcgtct ttgaatacta
     4981 tggtgacgct tatatcaaac agctacttat aaatgacgag ggggcatttc tgcacagttt
     5041 aaatagcaag tatgaagata taccgataga tagagatagt gattttagga ttattggtga
     5101 agttgttgga agttattcgg gaaatcatag ctaacgaagc tacggttgtg aatgagttta
     5161 ggagaaaata tggaaaatga agttttacat gcaactcacg agggtaaatt agtcttagat
     5221 aatacaactc ttgatgtagc agttttaaat aacggtcagc gaatcatcac ccaagctggt
     5281 gtttttaaag cattggacag acctagtaga ggcaataccc gtgtgatcgg aatacctact
     5341 tttatggatg caaaaaacct tcaaccattg atatcaaagg atttaagggg tgtgatcaac
     5401 aaaattgaat atagggattt aaatggtagg attcaattag gttttgatgc taacatttta
     5461 ccgctagtat cagatttata tttaaaagct agagaaattg gagctatcaa atcagaagct
     5521 cagatgagaa ctgcacaaaa agcggaaatg ttagttcgct cacttgcgaa agttggtgtt
     5581 actgctttag tagatgaagc tactggttat caatatgaga gagaacgttt tgagcttcaa
     5641 aaaatattta aagcatatat tagtgaagaa ttgctaaaat ggcaaaagat gtttccagat
     5701 gatttttata tggaaatttt tagattgaac gggtggagct acgatgttca atcaattaaa
     5761 aggcgtccag gtgttgttgg taaatggaca aacgaattga tttataaaag actgcctgat
     5821 ggagtgttag aagaattaaa aagaagaact ccaaaaacaa agaaaggtca atataaagct
     5881 agatttttcc aaactttaac accagatata ggacatcctg atttaaacgc tcaaatttat
     5941 aaagttcttg ggattatgag aatttcatct aactggaaag atttcaaaga gaaatttaat
     6001 gttatgacat ctagagaaga cggtataatt gaattagatt tagaatttga tgaagacaaa
     6061 taaaaaaagc cccacgctct caaagtttgg cgactctgag cgtgaggcaa aaccgtataa
     6121 gaaacaacca ttaaaaaggt cgttttcttg tacccatttt aacaaattga aagatggtat
     6181 gcaatgaaaa ttaaatcata taaaaaggaa aatggtgaaa ctgcttataa atttcttttg
     6241 tatgccggtt atgttaatgg aaaaagaaaa tatattaggc gagaaggttt caaaactaag
     6301 caggctgcaa gggaaacctt aattagttta caagctgaac ttgataaacc taaatcaagt
     6361 atgacatttg gagcattgac agatcaatgg ctaaaggaat atgaaaaaac cgttcagtgc
     6421 agtacctact taaaaacaga aagaaatatt aataaacata ttttgccaaa acttgataaa
     6481 gtgaagattg gagacatcaa cccactactt atccagcggc ttactgaaga atggtgcaac
     6541 gatttaaaat atggaggaaa aattcttggg cttgttagga atatcttaaa tctagctgtt
     6601 agatacggat atatcaataa caatccagct ttgccaatta cacctccaaa aataaaaagg
     6661 agaagaaaaa tgaataataa tttttataca cttgatcaac ttaaacaatt ccttgaacta
     6721 gttgaaaaaa ctgacaacat tgaaaaaata gccttgttta gattattagc atttactgga
     6781 atacgaaaag gggagcttct ggcactaact tgggatgatt tgaatggtaa tactctatca
     6841 attaataaag ctgtcacacg tactcaagtt ggactagaaa tagatgttac gaagacaaaa
     6901 tcgagcgata gattaatcag cttagatgat gaaactttgg aaattttact agaacttcat
     6961 gaaacttttc ctacttctac tcttatgttc caatctgaat caggtggaat tatgacgcca
     7021 agtttaccac gaaaatggct attgcaaatt atcaaaggga cagacttacc acaaatcaca
     7081 attcatggtt tcaggcacac tcatgcaagc ttacttttcg aatcaggtct atccttgaaa
     7141 caggtgcaac atagattagg gcatggagat ttacagacaa ctatgaacgt atatactcac
     7201 atcacgcaat cggcaattga tgacattgga actaaattca atcaatttgt tactaacaag
     7261 caactagatt gacaactaat tctcaacaaa cgttaattta acaacattca agtaactccc
     7321 accagctcca tccatgctta ccgtaagtaa tcataactta ctaaaacctt gttacatcaa
     7381 ggttttttct ttttgtcttg ttcatgagtt tccgttatcc ctatagtcat accatcacac
     7441 ttaccgatac tccatcaata ccactcaaaa ccttgtcata tcagagcttt ttgaccattt
     7501 ttttcatgaa tatctaaaaa aaagaatcaa aatagtacta aattccccat agcgcatgcg
     7561 ttatggggaa tcatagctat atcttgttta taatttatga tataatacag caaaaatagt
     7621 ttaggaaata acgtatgaca aaaaaacact tactaacact tcttctcatc tcttttttta
     7681 ctagcttttt ggtagcttgt tcaacaacga aagataaaga gcctcaaccg tctgattcag
     7741 aaatcattac tccccgacta caccaagccg ctcatcaaga taaacgcgct aactttgaaa
     7801 aaattaaact tgcgactgtt gattcctcat ttacaggagg aacaagcctt gaagaactta
     7861 tttcactctt tggagagcct agccaacatg atccaaaaac agcaggcgaa gtaacaatcg
     7921 acgcttatac ttggcagttt gatcaagtta ctctcactgt taatctttat caaaatagta
     7981 gtattgttaa aaccatctct aattttacct ttgcaagaga gttaggctta tcgcaaaagg
     8041 aataccaaca attacaaaaa ggaatgtctt atgaagacgt taaaaagatc ttaacagaac
     8101 ctgataatta tagccaagcg tcatctagtg atcatcaaac tttgcaagcg atttgggtta
     8161 gtggcttaaa gacagataca agcggagcta atatttctct cgtttttgaa aataatcagt
     8221 taacagaaat gtctcaggta ggacttgaag aataaaaaat gagtaatata ttttgctaa
//
LOCUS       DAUDYX010000067         7224 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.068, whole genome shotgun sequence.
ACCESSION   DAUDYX010000067 DAUDYX010000000
VERSION     DAUDYX010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7224
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.068"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(712..1635)
                     /locus_tag="VHD02_001702"
     CDS             complement(712..1635)
                     /locus_tag="VHD02_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5498292.1"
                     /translation="MSEKLVEVKDLEISFGEGKKKFVAVKNANFFIKKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSSGQILYDGKVINGRKSKSEANELIRKIQMIFQDPAASLNE
                     RATVDYIISEGLYNFNLFKTEEERKEKIKNMMAEVGLLSEHLTRYPHEFSGGQRQRIG
                     IARALVMNPEFVIADEPISALDVSVRAQVLNLLKRMQAEKGLTYLFIAHDLSVVRFIS
                     DRIAVIHKGVIVEVAETEELFNNPIHPYTQSLLSAVPIPDPILERQKELVVYHPDQHD
                     YTLDKPSMVEIKPNHFVWANQAEIEKYQKEL"
     gene            complement(1628..2698)
                     /locus_tag="VHD02_001703"
     CDS             complement(1628..2698)
                     /locus_tag="VHD02_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5498293.1"
                     /translation="MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFV
                     GESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQELTDLKTNKEWAKIRGSKIATIFQ
                     DPMTSLSPIKTIGRQITEVIIKHQKVSNAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
                     SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITH
                     DLGVVASIADKVAVMYAGEIVEFGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAI
                     PGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAKTWLLHPEAPKVQKP
                     EVIQDLHQKILRKMSQQEEGNV"
     gene            complement(2707..3633)
                     /locus_tag="VHD02_001704"
     CDS             complement(2707..3633)
                     /locus_tag="VHD02_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009569326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ5498294.1"
                     /translation="MESIDKSKFRFVERDSEASEVIDTPAYSYWKSVFRQFFSKKSTV
                     FMLVILVTVLMMSFIYPMFANYDFNDVSNINDFSKRYIWPNAEYWFGTDKNGQSLFDG
                     VWYGARNSILISVIATLINITIGVVLGAIWGVSKAFDKVMIEIYNIISNIPSMLIIIV
                     LTYSLGAGFWNLILAFCITGWIGVAYSIRVQILRYRDLEYNLASQTLGTPMYKIAVKN
                     LLPQLVSVIMTMLSQMLPVYVSSEAFLSFFGIGLPTTTPSLGRLIANYSSNLTTNAYL
                     FWIPLVTLILVSLPLYIVGQNLADASDPRSHR"
     gene            complement(3633..5135)
                     /locus_tag="VHD02_001705"
     CDS             complement(3633..5135)
                     /locus_tag="VHD02_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ5498295.1"
                     /translation="MKKYILNRIMRSLVSVVLVTALTYTIVYTLVPTSLIFKQDPNYN
                     KMTTTPDKKVAYENLTFQRMGYVNYFSSKELKDNASKVDSSVTTEATSANKAIYKKYI
                     DSLGNGWQLKRFPTSKQFYAIRNIPIYERVWNFFSNLVVIDHPWKIQDKDNPKLARYI
                     RLEKDKSVGWSLVGSGTKHKYLLYTNGKFPYLHQNFVTLNLGTSYPTYSNIPVLQVIS
                     QGQGRTALQDVTFPSGVTKKSSVDIYSRSYKNPKSLDDITKVNYGKGDSYTKTINNYV
                     DPSMIHNSFVIGFFGVMFSYIVGLPLGLFMARFKNTYFDRFSTATMTFMLALPSIAVI
                     YVVRFLGGMVGLPDSFPMLGASDPKSYILPALILGILNIPTTVIWFRRYLVDLQASDW
                     VRFARSKGLSESEIYRGHLFKNAMVPIVSGVPASIILAIGGATLTETVFAFPGMGKML
                     IDSIKSANNSMIVGLTFIFTVLSIVSLLLGDIVMTLVDPRIKLSTKKGGK"
     gene            complement(5200..7170)
                     /locus_tag="VHD02_001706"
     CDS             complement(5200..7170)
                     /locus_tag="VHD02_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ5498296.1"
                     /translation="MKKSKWLAAVSVAILSVSALAACGNKNASGGSEATKTYKYVFVN
                     DPKSLDYILTNGGGTTDVITQMVDGLLENDEYGNLVPSLAKDWKVSKDGLTYTYTLRD
                     GVSWYTADGEEYAPVTAEDFVTGLKHAVDDKSDALYVVEDSIKNLKAYQNGEVDFKEV
                     GVKALDDKTVQYTLNKPESYWNSKTTYSVLFPVNAKFLKSKGKDFGTTDPSSILVNGA
                     YFLSAFTSKSSMEFHKNENYWDAKNVGIESVKLTYSDGSDPGSFYKNFDKGEFSVARL
                     YPNDPTYKSAKKNYADNITYGMLTGDIRHLTWNLNRTSFKNTKKDPAQQDAGKKALNN
                     KDFRQAIQFAFDRASFQAQTAGQDAKTKALRNMLVPPTFVTIGESDFGSEVEKEMAKL
                     GDEWKDVNLADAQDGFYNPEKAKAEFAKAKEALTAEGVTFPVQLDYPVDQANAATVQE
                     AQSFKQSVEASLGKENVIVNVLETETSTHEAQGFYAETPEQQDYDIISSWWGPDYQDP
                     RTYLDIMSPVGGGSVIQKLGIKAGQNKDVVAAAGLDTYQTLLDEAAAITDDNDARYKA
                     YAKAQAYLTDNAVDIPVVALGGTPRVTKAVPFSGGFSWAGSKGPLAYKGMKLQDKPVT
                     AKQYENAKEKWMKAKAKSNAKYAEKLADHVEK"
BASE COUNT         2244 a         1410 c         1341 g         2229 t
ORIGIN      
        1 actttttttg tactttttta tttgcccgcg ttgtaaaatg aagtgcaaca aaaaagagga
       61 aaagctttga actacacccc aaacgttaga caaaaaaatc taacgttcgg ggtatttttt
      121 atgaaattaa gtgatgaagg tacgaaatag acatcgttaa gaaagaaaaa aatacctact
      181 atccaccaga gataaagcaa gaaataatag ataacgtttt gatagaaggc ccgccacaat
      241 accaagtatc acttgtttat ggcttgttac atgcaggaac tctcccaagt tgaatagcac
      301 aatataagaa aaacagttat actgttgaga aaacaagagc aagaccatca caaacaggac
      361 gtaaaccaaa agaaacctga agaaatgact aaattagagc ggcctcaaga agaaaatgaa
      421 tatctcagaa ctgaggttgc ttacttaaaa aaccgaggga actccgtttg aaagaagcat
      481 ctctgcaaag aagaagacaa aaaaccatta taaagaatgg acaaagacga gctctgatta
      541 gattttcttt taaggacggc taaattgact cgttctacct actattatca tcgattattg
      601 caacaataaa cgtatcaaaa taaaacgaaa aggacttagt cctctacaac acagaactaa
      661 atcctttact tcaaatagag tagccaactt ttggggctca gtgcatctct tttacaattc
      721 tttttgatat ttttcaattt ctgcttggtt tgcccaaaca aagtgatttg gtttgatttc
      781 aaccattgat ggcttatcta atgtataatc atgctggtct ggatgataga caacaagttc
      841 tttttgacgc tctaaaattg gatctgggat aggcacggct gataacaaag attgggtgta
      901 gggatgaatt gggttattaa acagttcttc tgtttctgca acttctacaa taaccccttt
      961 atggataacc gcaatacgat ctgaaataaa gcgaacgact gaaagatcat gggcaatgaa
     1021 gagataagtc aaaccttttt cggcttgcat tcttttaaga agattcaaga cctgtgcgcg
     1081 aacggaaacg tccaaagctg aaatcggttc atcagcaata acgaattcag ggttcattac
     1141 tagggctcta gcgataccga ttcgctgacg ttgacctcct gaaaattcat gagggtagcg
     1201 cgtcaaatgc tctgatagca aaccaacttc ggccatcatg ttcttaattt tttctttacg
     1261 ttcttcctct gttttaaaca gattaaagtt ataaaggcct tctgaaatga tgtagtcaac
     1321 ggttgcccgt tcattcaaac tagcagcggg atcttggaaa atcatttgaa ttttacgaat
     1381 gagctcattg gcttctgatt ttgattttct gccattaatt actttcccat cgtataaaat
     1441 ttgacctgaa ctagtatcgt tcaaaccaat aatagcacga ccaattgttg ttttaccact
     1501 cccagactct ccaactaaag aaaaggtttc tcctttttta ataaagaaat tagcattttt
     1561 aactgcaaca aattttttct ttccttctcc gaaggaaatt tctaggtctt ttacttcgac
     1621 taatttctca gacatttcct tcctcctgtt gtgacatttt ccttaagatt ttttgatgca
     1681 aatcttgaat gacttctggt ttttgaactt ttggagcctc tgggtgtaaa agccatgttt
     1741 tggcccaatg agtctcagat acgttaatcg ccggaggtgc tttttcaaag tctaaaacca
     1801 tagcatattc tgagcgaagt gcaaaggcat ctccgataat tggtgaataa agtgatggag
     1861 gcgttcctgg aatagcgtaa agttcaccag attcatctgc caactgcggt aagctagaca
     1921 gcaaactcca tgtatagggg tgtcttggat catagaaaat ctcttcgact gttccaaatt
     1981 caacaatttc tcctgcatac atgacagcca ctttatctgc aatgcttgcc acaacaccta
     2041 aatcgtgcgt aataaagata atggtgaaat gatattctcg ttgaagcgac ttcaataact
     2101 caacgatttg agcttgaata gtcacatcaa gggctgttgt tggctcatca cagataagaa
     2161 tatctgggcg acaagctaaa gcaatagcga taacaatacg ttgacgcatt cctcctgaat
     2221 actcaaatgg gtaatcttca aagcgttttt tggcatttgg gatacccact ttattcatgt
     2281 aatcaagggc catttcttta gctttggcat tacttacttt ttggtgctta ataatcactt
     2341 ctgtgatttg tctaccgata gttttaatgg gactaagact ggtcattggg tcttggaaaa
     2401 tcgttgcgat ttttgagccg cgaatctttg cccactcttt atttgttttt aaatctgtca
     2461 attcttgccc acgatagaca attgaaccat tagcaatgcg tccattagac tccaacatcc
     2521 ctgtaaaggt ctttgttaaa acagatttac ctgagcctga ttcccctaca aaagcaagga
     2581 cttctccttc aacaagttcc agtgagacgt tacggatagc tgttaaaaca cgatcacgca
     2641 catcaaattc taccaccaca tctttggcag ttaagattac attattttct tttgtcattt
     2701 taactcctat ctatgtgaac gtgggtcact ggcatcagcc aagttttgtc cgacaatgta
     2761 tagtggtaac gatactaaaa tcaatgttac taagggaatc caaaagaggt aggcatttgt
     2821 tgttaagttg cttgaataat tagcaatcaa acgtcctaaa cttggagtgg tggttggtaa
     2881 accaatccca aagaaggata agaaggcctc agaagataca taaactggta gcatttgtga
     2941 caacatagtc atgataactg aaaccaattg aggcaggagg ttcttaacag caatcttgta
     3001 catgggtgtt cccaaagttt gactagcaag gttgtattct aaatcacggt aacgcaagat
     3061 ttgaacacgg atggagtagg cgacaccaat ccatccagtg atacagaaag ctagaatcaa
     3121 attccaaaat cctgcaccta atgaataagt caaaacaatg ataataagca tagaagggat
     3181 atttgagata atgttataaa tttcaatcat aactttatca aatgctttag aaactcccca
     3241 tatggctcct aacactaccc caatggtgat attaattagt gtcgctataa ctgagattaa
     3301 aatagaatta cgtgccccat accaaacacc atcaaacaga gattgcccat ttttgtcggt
     3361 tccaaaccag tactctgcat ttggccaaat ataacgcttt gaaaagtcat tgatattact
     3421 aacgtcatta aagtcgtagt tggcaaacat tggataaata aagctcatca tcaagactgt
     3481 cactaaaatc acgagcataa agactgtaga ttttttagaa aaaaactgac gaaacactga
     3541 tttccagtaa gaataagcag gggtatcaat cacttcggag gcttcactat cgcgctcaac
     3601 aaatcgaaat ttagatttat caatcgattc cattacttac cccctttttt tgttgacaat
     3661 ttaatacgtg gatcgacaag ggtcatgaca atatctccta gtaaaagtga aacaattgac
     3721 aagacagtga agataaaggt taatccaaca atcatagaat tgttagcaga tttaatagaa
     3781 tcaattaaca ttttacccat acccgggaag gcaaagacag tctctgttaa ggttgcacca
     3841 ccaatagcaa ggataatact tgcaggaacc cctgacacaa ttgggaccat ggcatttttg
     3901 aagagatgcc ctctgtaaat ttcagattct gataatcctt ttgaacgcgc aaaacgtacc
     3961 caatcgctgg cctgaaggtc aacaagatag cgacggaacc agataaccgt agttggaata
     4021 tttaaaattc ctaaaataag ggctggtaaa atataagatt taggatcaga tgcccccagc
     4081 attgggaaac tatctggtaa accaaccatg ccacctagga agcgaacaac atagataaca
     4141 gcaatacttg gtaaagcaag catgaaggtc atggtcgctg ttgagaaacg gtcaaaatag
     4201 gtatttttaa accgagccat aaataaacca agaggcaaac caacgatata ggaaaacata
     4261 acaccaaaaa aaccaattac aaaagaattg tgaatcattg atgggtcgac ataattattg
     4321 atggttttag tgtaggaatc tcccttacca tagtttacct tggtgatatc atccaatgat
     4381 tttggatttt tgtagctacg gctatagata tccactgacg atttcttagt gacacctgat
     4441 gggaaggtca cgtcttgaag agctgttcgt ccttgccctt gtgaaataac ctgaagaaca
     4501 ggaatattgc tgtatgttgg ataagatgtt cctaagttta gggtaacaaa gttttggtga
     4561 agataaggaa attttccgtt agtatagagg agatatttat gttttgtccc cgaaccaaca
     4621 agtgaccagc caactgattt atctttttct agacgaatat acctagctaa ttttggatta
     4681 tctttatcct gaatcttcca agggtgatca ataacaacta gatttgagaa aaagttccaa
     4741 acacgttcgt aaataggaat attgcgaatt gcataaaact gtttgctagt ggggaagcgt
     4801 tttagctgcc agccattgcc aagagaatca atatacttct tatagatagc tttgttagca
     4861 ctagttgctt ctgtcgttac actactatct actttagaag cattatcttt taattctttg
     4921 cttgaaaaat aattgacata acccatacgt tgaaaagtta gattttcata cgctactttt
     4981 ttatctggtg ttgttgtcat tttattataa ttagggtctt gtttaaagat aaggctggta
     5041 ggtactagag tatatacgat ggtgtatgtt aaagccgtta ccaaaactac cgacactaac
     5101 gatcgcatga tacggtttaa aatgtatttt ttcatagtta tcctcaatag ttcaaaagaa
     5161 ctccctcaca taaacgagaa agttcttttg aacatcgttt tatttttcaa cgtgatcagc
     5221 taacttctca gcatattttg catttgactt agcctttgct ttcatccatt tttcttttgc
     5281 attttcgtat tgttttgctg tgacaggttt gtcttgaagt ttcattcctt tatatgctag
     5341 aggaccttta gaccctgccc aagagaagcc cccgctaaat ggaacggctt tagtaactcg
     5401 tggagtgcca cccaatgcca caactggaat atctacggca ttatctgtaa ggtaggcttg
     5461 tgcttttgcg taagctttat agcgcgcatc gttgtcgtct gtaattgctg ctgcttcatc
     5521 aagaagagtt tggtaggtat caagacctgc agctgccaca acatccttat tttgacctgc
     5581 tttgattcca agtttttgga taacagatcc accacctact ggactcatga tgtcaaggta
     5641 ggtccgtgga tcttggtagt ctggtcccca ccatgatgaa atgatatcgt agtcttgttg
     5701 ttctggggtc tcagcataga agccttgggc ttcgtgagtt gatgtttctg tttcaagaac
     5761 attgacaatg acattttctt taccaagaga tgcttcaaca gattgtttga aagactgagc
     5821 ttcctgaaca gttgctgcgt ttgcttggtc aacagggtaa tctaattgaa ctgggaaggt
     5881 tacaccttca gctgtcaaag cttctttggc ttttgcaaac tcagcttttg ctttttcagg
     5941 attatagaaa ccatcttgag catcagctaa gttaacgtct ttccattcat caccaagttt
     6001 tgccatttcc ttttcaactt ctgaaccaaa atcactttct ccaatggtca caaatgttgg
     6061 tggcacaagc atgttacgta aggcttttgt tttggcatct tgacctgcag tttgtgcttg
     6121 gaatgacgct cggtcaaaag caaactgaat agcttgacga aaatccttgt tgttaagagc
     6181 tttcttaccg gcatcttgtt gtgcagggtc tttcttagtg tttttgaaag aagtgcggtt
     6241 caaattccat gttaaatgac ggatatctcc agtcaacatt ccgtaagtaa tgttatcagc
     6301 atagtttttc ttagctgatt tgtaggtagg gtcatttggg taaagtcgtg caacgctgaa
     6361 ctcacccttg tcaaagttct tgtagaacga acctgggtct gaaccatctg agtaagtcaa
     6421 tttaacagat tctatcccaa cattcttagc atcccagtag ttttcatttt tatggaattc
     6481 catagatgat tttgaggtga aggcgctcaa gaagtaagca ccattaacaa ggattgatga
     6541 tggatcggtt gtaccaaaat ctttaccttt tgacttcaaa aatttcgcat taactgggaa
     6601 aagcacacta taagttgttt ttgaattcca gtagctttca ggcttgttca aagtatactg
     6661 aacagtttta tcgtcaaggg ctttgacacc aacttcttta aaatctactt caccattttg
     6721 gtaagccttt aagtttttta ttgaatcttc aacaacgtaa agagcatctg atttatcgtc
     6781 aaccgcgtgc ttcaaaccag tcacaaaatc ttctgctgtt actggggcat attcttcacc
     6841 atcagccgta taccaagaga caccatcgcg aagagtataa gtataagtca gaccgtcttt
     6901 tgaaaccttc caatctttag caagtgatgg tactaaatta ccatactcat cgttttccaa
     6961 aagaccatca accatttgtg ttatcacatc agtcgttcca ccgccattag tcaaaatata
     7021 atccaatgat tttggatcgt taacaaaaac gtacttgtag gtttttgtag cttctgagcc
     7081 acctgaagca tttttattac cacaagctgc caaagcggat actgacaaga tcgcaacact
     7141 tacagctgcc aaccatttac ttttcttcat aaaagtcacc cctaaacttt atttttcatt
     7201 ttttggcaat tgctaaatga aatt
//
LOCUS       DAUDYX010000068         7095 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.069, whole genome shotgun sequence.
ACCESSION   DAUDYX010000068 DAUDYX010000000
VERSION     DAUDYX010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7095
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.069"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(176..1435)
                     /locus_tag="VHD02_001707"
     CDS             complement(176..1435)
                     /locus_tag="VHD02_001707"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEQ5498297.1"
                     /translation="MRKIIINGGKALSGEVAVSGAKNSVVALIPAIILADDIVILDGV
                     PAISDVDSLIEIMELMGATVNYHGDTLEIDPRGVQDIPMPYGKINSLRASYYFYGSLL
                     GRFGQAVVGLPGGCDLGPRPIDLHLKAFEAMGVEVSYEGENMNLSTNGQKIHGAHIYM
                     DTVSVGATINTMVAATKAQGKTVIENAAREPEIIDVATLLNNMGAHIRGAGTDIITIQ
                     GVQKLHGTRHQVIPDRIEAGTYIALAAAIGKGVKITNVLYEHLESFIAKLEEMGVRMT
                     VEEDAIFVEKQESLKAITIKTSPYPGFATDLQQPLTPLLLKADGRGTIIDTIYEKRIN
                     HVPELMRMGADISVIGGQIVYQGPSRLTGAQVKATDLRAGAALVTAGLIAEGKTEITN
                     IEFILRGYASIIAKLTALGADIQLIED"
     gene            complement(1609..2805)
                     /locus_tag="VHD02_001708"
     CDS             complement(1609..2805)
                     /locus_tag="VHD02_001708"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEQ5498298.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAEDPEAHVAAE
                     TCVYTGSVHVFGEISTTAYIDINRVVRDTIAEIGYTEAEYGFSAESVGVHPSLVEQSG
                     DIAQGVNEALESREGDTDDLSHIGAGDQGLMFGFAINETPELMPLPISLSHQLVRRLA
                     ELRKSGEISYLRPDAKSQVTVEYDEHDKPVRVDTVVISTQHDPEATNDQIRQDVIEKV
                     IKAVIPADYLDDDTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSG
                     KDATKVDRSASYAARYIAKNLVAAGLATKAEVQLAYAIGVAQPVSVRVDTFGTSTVPE
                     AVLEAAVRQVFDLRPAGIIQMLDLKRPIYKQTAAYGHMGRTDIDLPWERLNKVDALVE
                     AVKTVL"
     gene            complement(3341..5719)
                     /locus_tag="VHD02_001709"
     CDS             complement(3341..5719)
                     /locus_tag="VHD02_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEQ5498299.1"
                     /translation="MKTKKVIILVGLLLSSQLTLISCQSRGNGTYPIKTKQSRKGMTS
                     NKIKPIKKSKKTNKTHKGVAGVDFPTDDGFILTKDSKILSKTDQGIVVDHDGHSHFIF
                     YADLKGSPFEYLIPKEASLAKPAVAQQAASQGTSKVADPHHHYEFNPADIVAEDALGY
                     TVRHDDHFHYILKSSLSGQTQAQAKQVATRLPQTSSPVSTATANGIPGLHFPTSDGFQ
                     FNGQGIVGVTKDSILVDHDGHLHPISFADLRQGGWAYVADQYDPAKKAEKPAETPHTP
                     ELSEREKEYQEKLAYLAEKLGIDPSTIKRVETQDGKLGLEYPHHDHAHVLMLSDIEIG
                     KDIPDPHAIEHARELEKHKVGMDTLRALGFDEEVILDIVRTHDAPTPFPSNEKDPNKM
                     KEWLATVIKLDLGSRKDPLQRKGLSLLPNLETLGIGFTPIKDISPVLQFKKLKRLLMT
                     KTGVTDYRFLDNMPQLEGIDISQNNLKDISFLSKYKNLTLVAAADNGIEDIRPLGQLP
                     NLKFLVLSNNKISDLSPLASLHQLQELHIDNNQITDLSPVSHKESLTVVDLSRNADVD
                     LATLQAPNLETLMVNDTKVSHLDFLKNNPNLSSLSINRAQLQSLEGIEASSVIVRVEA
                     EGNQIKSLVLKDKQGSLTFLDVTGNQLTSLEGVNNFTALDILSVSKNQLTNVNLSKPN
                     KTVTNIDISHNNIPLADLKLNEQHIPEAIAKNFPAVYEGSMVGNGTAEEKAAMATKAK
                     ESAQEASESHDYNHNHAYEDEEGHAHEHRDKDDHDHEHEDENEAKDEQNHAD"
     gene            5923..6864
                     /locus_tag="VHD02_001710"
     CDS             5923..6864
                     /locus_tag="VHD02_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEQ5498300.1"
                     /translation="MKTSEKIYQLLSQTDDFVSGEYLADQLSISRTSVWKSIKSLENQ
                     GIQIDSLKHKGYRMVQGDILLPKTISQGLGMPVTYTPHSQSTQLDAKQGIEAHNSAPR
                     LYLAPSQEAAKGRLDRQFFSASTGGIYMSMYLKPNVPYADMPPYTMMVASSIVKAISR
                     LTGIDTEIKWVNDIYLGNHKVAGILTEAITSVETGLITDVIIGVGLNFFVTDFPEAIA
                     QKAGSLFTEKPTITRNDLIIDIWKLFLSIPVKDHVKVYKEKSLVLNKQVTFIENSQEK
                     RAIAIDLTDQGHLIVQFENGDFQTLRSGEISLSSWEN"
     gene            complement(6839..7042)
                     /locus_tag="VHD02_001711"
     CDS             complement(6839..7042)
                     /locus_tag="VHD02_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEQ5498301.1"
                     /translation="MTIRQFLFMAFVCAFETYFFNDLLLSGNYLFALFWGLLLFRDLR
                     RVHTINQLTKTILKTANSPKKKD"
BASE COUNT         2104 a         1505 c         1265 g         2221 t
ORIGIN      
        1 tcactttact accgtcaact cattatttta gcattaaaat taataatatt agttagtgat
       61 atcattttta tattttttat gacagttaaa aagccataaa atgcaacaaa gtatgcacct
      121 aagtatcatt agcctaagca catgtaatat aaaaaagaga agcgttagct tctctttagt
      181 cttcgattaa ttgaatatct gcccctagtg ctgttaattt tgcaatgata ctagcatagc
      241 cacgtaagat aaattcaata ttagtaattt ctgtcttgcc ttcagctatt agaccagcag
      301 tcactagagc cgctccggca cgtaaatcag tggctttgac ctgagctcct gtcaggcgag
      361 acggcccttg ataaacaatc tgaccaccaa taactgagat atctgctccc atgcgcatca
      421 actctggcac atggttaatg cgcttttcat atatagtgtc aataatcgtt ccacgaccat
      481 ctgcttttaa caacaagggc gttaatggct gttgcaaatc agtggcaaaa ccagggtaag
      541 gagaggtttt aattgtaata gccttgaggg attcttgctt ttcaacaaag atagcatcct
      601 cttctaccgt catacggaca cccatttctt ctaacttagc aatgaaactc tctaaatgct
      661 cataaagaac attagtgatc ttaacacctt ttccaatagc tgccgccaaa gcgatataag
      721 tccctgcttc aatacgatca ggaataactt ggtgtctagt accatgcaat ttctgaacac
      781 cttgtatcgt aataatatct gtacctgctc cacgaatatg cgcacccatg ttattcaaaa
      841 gagtagccac atcaataatc tctggctcac gtgcagcgtt ttcaataacc gttttacctt
      901 gagcttttgt tgccgcaacc atagtattaa ttgtagcacc gacactaacg gtgtccatat
      961 agatatgagc gccatgaatt ttttgaccat ttgtcgataa attcatattt tcaccctcat
     1021 aagacacttc aactcccata gcctcgaagg ccttcaaatg caagtcaatt ggtcttggtc
     1081 ctaaatcgca accaccaggt aagccaacaa ctgcttgtcc aaaacgacct aaaagactac
     1141 cgtagaaata ataggaagct cgaagactat taatctttcc gtaaggcatc gggatgtctt
     1201 gcacccctct agggtcaatt tctaaagtat caccatgata gtttactgtt gctcccataa
     1261 gctccatgat ttcaatcaga ctatctacat cactaatagc aggtacacca tcaagaatca
     1321 caatatcatc ggctaagatg attgctggaa taagcgcaac aacactgttt tttgcccctg
     1381 aaactgccac ttctccagat aaggcttttc cgccattaat aataatttta cgcatacatt
     1441 caaaactttc ttatactaga attcaacctt ttcattttat cataaatcat gaagcctgac
     1501 aattattgac gtcttaattt ttaatcatcc cttttaaagg cttatttaat aacaaaaaag
     1561 cagagtaaga ttttttcact tactctactt ttttaataaa gttgtcgttt ataatactgt
     1621 ttttacagct tctactaaag catcaacctt atttaaacgc tcccatggca aatcaatatc
     1681 tgttctcccc atgtgaccat aggcagctgt ttgtttgtaa atcgggcgtt ttaaatcaag
     1741 catttgaata atgcctgctg gccgtaaatc aaagacttga cgaacagcag cttctaaaac
     1801 agcttcagga accgttgagg ttccaaatgt atccacgcga acagacacag gctgagccac
     1861 cccaatcgca taagctagtt ggacttcagc tttggtcgca aggcctgctg ccacaaggtt
     1921 tttagcaata tagcgagcag cgtaagaagc cgaacggtcc acttttgtcg catcttttcc
     1981 tgaaaaagca ccgccaccat gacgtgaata gcctccatag gtatccacaa taatcttacg
     2041 tccggtcaat ccagagtctc cttgcggacc tccaataaca aaacgtcctg ttggattaat
     2101 gaagaacttt gtgtcatcat ctaaataatc tgctggaatt acagccttga tcactttttc
     2161 aatcacatcc tgacgaattt ggtcattagt agcttctgga tcatgctgag tagaaataac
     2221 gactgtatcc acacgaacag gtttatcgtg ttcatcgtac tcaactgtca cttgagattt
     2281 agcatctgga cgtagataac taatctcacc tgacttacgt aattccgcca agcgacgcac
     2341 taattggtga gataaggaaa ttggtaaagg catcaattct ggtgtttcat tgatggcaaa
     2401 accaaacatg agcccttggt cacccgctcc aatatggcta aggtcatcag tatcaccttc
     2461 tctgctttcg agagcttcat taaccccctg agcgatatct ccagattgtt ctacaagtga
     2521 tggatgtact cccactgatt ctgctgaaaa gccatattct gcttctgtat aaccaatttc
     2581 tgcaatggtg tcgcggacca cacggttaat atcaatgtat gctgttgttg agatttctcc
     2641 aaaaacatga acagaacctg tgtaaacaca agtttctgca gccacatggg cttctggatc
     2701 ttctgctaaa atggcatcta aaatagcatc tgaaatttgg tctgcaatct tatctggatg
     2761 cccttcagat acggactcag acgtgaaaag tttacgttct gacataaaaa tgtcccccct
     2821 aatatgttga ttcttccttt ttcagtcaca ttgaccacaa agcaagaagt tgcctacccc
     2881 tttcttccta gctgattgag caagtcaagt ggccaaaacc aacttagcta atcagttacg
     2941 gttttactag taaggcaact agctagtcca cctagtggct agcgtccatt aaccagacaa
     3001 agaatgtctg acaatagcgt tgagttacaa agaatccggt ctccttgttg agaggatgtc
     3061 accacaaaat aagccttaga cgaagtaatt tagctaaaga gagaacgact gttccccaaa
     3121 tgaagtcaac aacgtctcaa aacatttcgt tcaacagacc gtcttatcgg gactcaataa
     3181 caatctatta taacatgttt tagccattta ggtgccattc ttttcactca ttatcctatt
     3241 tatttcaaaa aaacctcaag gttattcttg aggttttctt gatagtcaac aatggtgtca
     3301 ttaagacgat tcagttggat cgggttattc ttagattagt ttagtcagca tggttttgct
     3361 catctttagc ttcattttca tcctcatgtt catggtcgtg atcatctttg tctctgtgct
     3421 cgtgagcatg accttcttca tcttcatagg catgattatg gttgtagtca tgtgattccg
     3481 atgcttcttg agcactttct ttcgccttag tagccatagc tgctttttct tcagctgttc
     3541 cattacctac catagaacct tcgtaaaccg ctgggaagtt tttcgcaatg gcttctggaa
     3601 tatgttgctc gttcaattta aggtctgcca atgggatatt gttatgacta atatcaatgt
     3661 tagtaactgt cttattgggt ttagataggt tgacatttgt taattggttt ttagacacgc
     3721 ttaaaatgtc aagtgctgta aaattattaa caccttctag agaagtcaac tggttgcctg
     3781 tcacatccaa gaaagtaagt gacccttgct tgtctttaag cacaagcgat ttaatttggt
     3841 taccttctgc ttctactctg acaatgacgc tacttgcttc aataccttca agagattgca
     3901 attgcgcacg gttaatagat aggctagata gattaggatt atttttcaag aaatccaaat
     3961 gagaaacctt ggtatcattg accattaacg tttctaagtt gggtgcttga agtgttgcta
     4021 agtcaacatc agcatttctt gataaatcaa caaccgtcaa tgattcttta tgagaaacag
     4081 ggcttaaatc tgtaatctga ttattatcaa tgtgcaattc ttgcaattga tgtaacgatg
     4141 ccagtgggct taaatcagaa atcttattat tactcaatac gaggaatttg agatttggta
     4201 attgaccaag cggcctaata tcttcaatac cattatcagc agccgctact agagttaagt
     4261 ttttatattt gctcaagaaa ctaatatctt tgagattgtt ttgtgaaata tcaatgcctt
     4321 ctaactgtgg catattatcc aaaaatctat aatcagtcac ccctgttttt gtcattagta
     4381 accgtttcaa ttttttaaat tgcaaaacag gtgagatatc tttgattggt gtaaagccaa
     4441 ttcctaaagt ttctaagttg ggtaacagtg aaagtccttt acgttgcaaa ggatctttac
     4501 ggctgcccaa gtcaagtttg ataaccgttg ctaaccattc tttcatctta ttcggatctt
     4561 tttcatttga tgggaatggg gttggagcat cgtgagtgcg aacgatatcc aaaatcactt
     4621 cttcatcaaa ccctaaggca cgcaaggtat ccattccaac cttatgtttt tccaattcac
     4681 gggcatgctc aatagcatgt ggatctggaa tgtcttttcc gatttcaata tcagataaca
     4741 tcaatacgtg tgcgtggtca tggtgagggt attccaaacc aagtttaccg tcttgtgttt
     4801 ccacacgttt aatagttgat ggatcaatcc ccaatttttc tgccaaataa gctaattttt
     4861 cttggtattc cttttcacgt tcagatagct ctggtgtgtg aggggtttct gctggctttt
     4921 ctgctttttt agcgggatcg tattgatctg ccacatatgc ccagccaccc tgacgaaggt
     4981 ccgcaaaaga aataggatgt aagtgaccat cgtggtccac taaaatactg tcttttgtta
     5041 ccccaacaat accttgaccg ttaaattgaa aaccatctga ggttgggaaa tgcaagcctg
     5101 gaataccatt agctgtagct gttgaaacag ggctactggt ttgtggcaag cgagtagcaa
     5161 cctgtttagc ttgtgcctgt gtctgacctg ataagcttga cttcaaaata taatggaagt
     5221 gatcatcgtg gcgaaccgtg tagcctaaag catcttcagc cacaatatcc gctgggttaa
     5281 attcataatg gtgatgagga tctgctactt tagaagtccc ttgactagct gcttgctgag
     5341 caacagctgg cttagctaaa cttgcttctt ttggaataag gtattcaaat ggacttccct
     5401 ttaaatcggc ataaaaaata aaatgcgaat gaccatcatg gtcaacaacg attccctgat
     5461 ctgtttttga taagattttt gagtctttgg ttaaaataaa cccatcatct gtaggaaaat
     5521 cgacacccgc cacacctttg tgagtcttgt ttgtcttttt gcttttttta atcggtttaa
     5581 ttttgtttga cgtcattccc ttacgtgatt gtttcgtttt aatgggatat gtaccattac
     5641 ctcgtgattg acaagatatc aaagtcaact gagatgataa caatagacca actaaaataa
     5701 taactttttt cgttttcatt caaccctcct ttttatttaa ctggttaagt atatcacatc
     5761 acttgttttt cctcaagctc tttttaggat aactattagt tgttgacgag ttggagctct
     5821 gaagcttcaa caaacaatag tttctgttgt tagtagcatt acacaaaaaa actaactttt
     5881 cttttccttt gaaaataaga tcttttataa tatactatac atatgaaaac atcagaaaaa
     5941 atttatcaat tactgtcaca aaccgatgac ttcgtcagtg gggaatatct agcagatcaa
     6001 ctcagtatct ctcgtacatc cgtttggaaa tccatcaaaa gtctcgaaaa tcaggggatt
     6061 caaattgatt ctttaaaaca caagggctac cgcatggtac aaggtgatat tttattaccc
     6121 aaaaccatta gccaagggct tgggatgcca gtcacctata cccctcacag ccaatctacc
     6181 caattagatg ccaaacaagg aatcgaagct cataacagcg ctccaaggct ttacctagct
     6241 cctagccaag aagctgccaa aggacgcctc gatcggcaat ttttttcagc tagcaccggt
     6301 ggtatttaca tgtccatgta cctaaaaccc aatgtccctt atgctgatat gcctccttat
     6361 acgatgatgg ttgcttccag tattgtcaaa gctatctcgc gattaacagg cattgataca
     6421 gaaatcaaat gggtaaacga catctatcta ggtaaccata aagttgcagg gattctgaca
     6481 gaagccatta cttctgttga aactggttta atcaccgatg tcattattgg ggttggtctc
     6541 aacttttttg tcaccgactt cccagaagcg attgctcaaa aggcaggttc tctctttacc
     6601 gaaaaaccga ctatcacgcg caatgacctt atcattgaca tctggaaatt attcttatct
     6661 attcctgtca aggaccatgt caaagtttac aaagaaaaat cacttgttct taataaacaa
     6721 gtgaccttta tagaaaatag tcaagaaaaa cgtgctattg cgattgatct cacagatcaa
     6781 ggacacctca ttgtacagtt tgaaaatggt gacttccaaa ccttgcgaag cggtgaaatc
     6841 agtctttctt cttgggagaa ttagccgttt ttaaaatggt tttagtcaat tggttaatgg
     6901 tatgcactct tcttaaatcc ctaaagagca acaagcccca aaataaggcg aagagataat
     6961 ttccagagag caacaggtca ttaaaaaaat aggtttcaaa agcacataca aaggccataa
     7021 aaagaaattg tcgaatagtc atagctttat tataacagaa aaaaatgatt ctatgaagaa
     7081 tccacacaga ttgat
//
LOCUS       DAUDYX010000069         6929 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.070, whole genome shotgun sequence.
ACCESSION   DAUDYX010000069 DAUDYX010000000
VERSION     DAUDYX010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.070"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            206..856
                     /locus_tag="VHD02_001712"
     CDS             206..856
                     /locus_tag="VHD02_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEQ5498302.1"
                     /translation="MKERLEHYSTSSIYSGFEVYLPLVLQTITDVIIAENIKSKKETG
                     FKLYEHFTSRIKSEASMIEKCQRKQLPLTSKSALKIIKDSIGIRIICGFIDDIYRMVD
                     LLKSIPGMSVNTEKDYILNAKPNGYRSYHLILELETHFPDILGGKKGCYFIEVQLRTI
                     AQDSWASLEHQMKYKHQVANAEMITRELKRCADELASCDVTMQTIRQLIQETTEEE"
     gene            859..1527
                     /locus_tag="VHD02_001713"
     CDS             859..1527
                     /locus_tag="VHD02_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ5498303.1"
                     /translation="MKILLAEDEWQMSNVLTTAMTHQGYDVDVVFNGQEAIDKAKDNA
                     YDIMILDIMMPIKSGIEALKEIRASGNCSHIIMLTAMAEINDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMERRVESFTPQVLQFAGVTLNINEQELSAGNAIRLASKEGKLMAFL
                     MLNQGKYLDTKTLYQHVWSDQEDYDTSIVWVYISYLRQKLLAIQANVFIAGDKDTSYC
                     LLEK"
     gene            1536..2768
                     /locus_tag="VHD02_001714"
     CDS             1536..2768
                     /locus_tag="VHD02_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEQ5498304.1"
                     /translation="MFNRIRIRFIMIASIAIFIILSSIVGIINTARCYQSQQEINRIL
                     HLISSNKGKLPGTTESSKRLGTKLSEDSLSQFRYYSVIFNANGHLLSSNTANISALDR
                     EEAQYFARLFAKSGEEKGSYRHQDSVYSYLITQLPNEEKLVVILDTTFYFRSVGDLLA
                     VSMMLAFGGFIFFVVLVSLFSGMVIKPFVQNYEKQRRFITNAGHELKTPLAIISANNE
                     LVELMTGESEWTKSTSDQVKRLTGLINQMITLARLEEQPDVVLHMVDFSAIAQDAAED
                     FKSLVLKDGKRFDLTIQPNIMIKAEEKSLFELVTILVDNANKYCDPKGLVKVSLTTIG
                     RRRKRAKLEVSNTYLEGKSIDYSRFFERFYREDESHNSKEKGYGIGLSMAESMVKLFK
                     GTITVNYKNDAIVFTVVI"
     gene            3072..3950
                     /gene="mvk"
                     /locus_tag="VHD02_001715"
     CDS             3072..3950
                     /gene="mvk"
                     /locus_tag="VHD02_001715"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEQ5498305.1"
                     /translation="MNENIGYGKAHSKIILIGEHAVVYGYPAIALPLTDIEVVCHIFP
                     ADKPLVFDFYDTLSTAIYAALDYLQRLQEPIAYEIVSQVPQKRGMGSSAAVSIAAIRA
                     VFSYCQEPLSDDLLEILVNKAEIIAHTNPSGLDAKTCLSDHAIKFIRNIGFETIEIAL
                     NGYLIIADTGIHGHTREAVNKVAQFEETNLPYLAKLGALTQALERAINQKNKVAIGQL
                     MTQAHSALKAIGVSISKADQLVEAALRAGALGAKMTGGGLGGCMIALADTKDMAEKIS
                     HRLKEEGAVNTWIQML"
     gene            3932..4876
                     /gene="mvaD"
                     /locus_tag="VHD02_001716"
     CDS             3932..4876
                     /gene="mvaD"
                     /locus_tag="VHD02_001716"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEQ5498306.1"
                     /translation="MDPNVITVTSYANIAIIKYWGKENQAKMIPSTSSISLTLENMFT
                     TTSISFLPDTATSDQFYINGVLQNDEEHTKISAIIDQFRQPGQAFVKMETQNNMPTAA
                     GLSSSSSGLSALVKACDQLFNTQLDQKALAQKAKFASGSSSRSFFGPVAAWDKDSGAI
                     YKVETDLKMAMIMLVLNAAKKPISSREGMKLCRDTSTTFDEWVEQSAIDYQHMLTYLK
                     TNNFEKVGQLTEANALAMHATTKTANPPFSYLTKESYQAMEAVKELRQEGFACYFTMD
                     AGPNVKVLCLEKDLAQLAERLGKNYRIIVSKTKDLPDV"
     gene            4869..5882
                     /locus_tag="VHD02_001717"
     CDS             4869..5882
                     /locus_tag="VHD02_001717"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEQ5498307.1"
                     /translation="MSNYCVQTGGKLYLTGEYAILTPGQKALIHFIPLMMTAEISPAA
                     HIQLASDMFAHKAGMTPDASYALIQATVKTFADYLGQSIDQLEPFSLIITGKMERDGK
                     KFGIGSSGSVTLLTLKALSAYYQITLTPELLFKLAAYTLLKQGDNGSMGDIACIAYQT
                     LVAYTSFDREQVSNWLQTMPLKKLLVKDWGYHIQVIQPALPCDFLVGWTKIPAISRQM
                     IQQVTASITPAFLRTSYQLTQSAMVALQEGHKEELKKSLAGASHLLKELHPAIYHPKL
                     VTLVAACQKQDAVAKSSGSGGGDCGIALAFNQDARDTLISKWQEADIALLYQERWGEN
                     DQP"
     gene            5869..6858
                     /locus_tag="VHD02_001718"
     CDS             5869..6858
                     /locus_tag="VHD02_001718"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEQ5498308.1"
                     /translation="MTNRKDDHIKYALKYQSLYNAFDDIELIHHSLPSYDLSDIDLST
                     HFAGQDFDFPFYINAMTGGSQKGKAVNEKLAKVAAATGIVMVTGSYSAALKNPNDDSY
                     RLHEVADNLKLATNIGLDKPVALGQQTVQEMQPLFLQVHVNVMQELLMPEGERVFHTW
                     KKHLAEYASQIPVPVILKEVGFGMDVNSIKLAHDLGIQTFDISGRGGTSFAYIENQRG
                     GDRSYLNDWGQTTVQCLLNAQGLMDQVEILASGGVRHPLDMIKCFVLGARAVGLSRTV
                     LELVEKYPTERVIAIVNGWKEELKIIMCALDCKTIKELKEVDYLLYGRLQQVN"
BASE COUNT         2210 a         1238 c         1425 g         2056 t
ORIGIN      
        1 agttaacgta atcgttatag atcaagttta ctatgatttt gtttgtaatt caatggtatt
       61 tttaaaaaat aacgtacaaa aaatatatga aagcgattta aaaacaaacg atacagatca
      121 tttgaagtta tatagttaga aataaagata accgatcatt gcagagattt aagcatttgt
      181 tgagaaaatt tgttataata agcgtatgaa agaaagacta gaacattatt ctaccagctc
      241 tatatatagc ggatttgaag tgtacttgcc gttggtacta cagacaataa cagatgtgat
      301 tattgctgag aatatcaaaa gcaaaaaaga aacggggttt aaactatacg aacactttac
      361 atcccgtatt aaatcggaag cttcgatgat tgaaaaatgt caacgcaaac agcttcctct
      421 aacaagcaaa tctgcgttaa aaatcattaa agatagtatc ggtattcgca ttatttgtgg
      481 ttttattgat gatatttatc ggatggtaga cttgctcaaa agcattcctg gtatgtcagt
      541 taacactgaa aaagattata ttttaaatgc caagcctaat ggctatagat catatcatct
      601 cattttggaa ttagaaacac attttccaga tattttaggg ggaaaaaaag gatgttattt
      661 tatagaagtg cagttgcgaa ccattgctca agactcttgg gctagcttgg aacatcaaat
      721 gaagtacaaa catcaagttg ctaatgcaga aatgattaca agagagttaa agcgttgtgc
      781 agacgagttg gcatcttgtg atgttaccat gcaaaccatt cgtcaattga ttcaagaaac
      841 gacggaggaa gagtaaccat gaaaattttg ttagctgaag acgaatggca gatgtctaat
      901 gttttgacaa ctgccatgac tcatcaaggt tatgacgttg atgtggtttt taacggtcaa
      961 gaggcaattg ataaagctaa agacaatgca tatgatatta tgattttaga tatcatgatg
     1021 ccgattaagt ctggcattga ggctttaaag gagattcgcg cttcaggaaa ttgctcacat
     1081 attattatgc tgacggctat ggcagaaatt aatgaccgtg ttactggact agatgcggga
     1141 gctgatgatt atctgactaa accgttctca ttaaaagaac ttttagctag attaaggtcg
     1201 atggaaagac gagtagaaag ttttactcct caggtattac aatttgctgg tgtcacatta
     1261 aatattaatg agcaagaatt gagtgccggt aatgctatac gattagcttc taaagaaggt
     1321 aaattaatgg cctttttaat gttaaatcaa ggaaagtact tggatactaa aacactttac
     1381 caacatgttt ggtcagacca agaagattat gatacttcta ttgtttgggt ttatatttct
     1441 tatcttcgtc aaaaattatt agctattcaa gcaaatgttt ttattgcagg tgataaagat
     1501 actagctatt gtttattaga aaaataggag gtgacatgtt taatcgtatc cgtattcgtt
     1561 ttatcatgat tgcttctatt gccattttta ttattttaag ttcaattgtt ggtattatta
     1621 acacggcacg ctgttaccaa agtcaacaag aaattaaccg tattttacat ttaatttcct
     1681 caaataaagg taaattacct ggcactacgg aaagctctaa acgacttgga acgaaactgt
     1741 ctgaagatag cctaagccag tttcggtatt atagtgtcat ttttaatgct aatggacatc
     1801 tactttcttc taatactgct aatatttcag ctttagatag agaagaagcc caatattttg
     1861 ctagactttt tgctaagtct ggggaagaaa aaggcagtta ccgtcaccaa gatagtgttt
     1921 attcgtactt gattactcag ctgccaaatg aagaaaaact ggttgttatt ttagatacta
     1981 ccttttattt tcgtagtgta ggagatttat tagccgtttc aatgatgttg gcctttggag
     2041 gatttatctt ttttgttgtt ttagtgagtc ttttttccgg tatggtcatc aaaccttttg
     2101 ttcaaaatta tgagaagcag cgccgcttta ttactaatgc aggccacgag ttaaaaacac
     2161 cactagccat tatctcagcc aataatgaat tggtcgaatt aatgactgga gaatcagaat
     2221 ggaccaagag tacaagtgat caggtgaagc gattgacagg cttgattaat caaatgatta
     2281 ctttggctcg tttggaggaa cagccagatg tggtattaca tatggtagac ttttcagcta
     2341 ttgctcaaga tgcagctgaa gattttaaga gtctggtttt gaaagatggt aaacgttttg
     2401 atttgacgat tcagccgaat attatgatta aagcggagga aaagtcatta tttgaacttg
     2461 tgacaatttt agttgataat gccaataaat attgtgatcc taaaggcttg gtcaaggtat
     2521 ctttaaccac tattggaaga agacgaaaac gagcgaaatt agaagtttct aatacctatt
     2581 tggaaggaaa atccattgat tacagccgct tttttgaacg cttttatcgc gaagacgaat
     2641 cccataatag caaagaaaaa ggttatggaa ttggtttatc tatggcagaa agtatggtta
     2701 agttatttaa aggaacgata actgtaaatt ataaaaacga tgctattgtt tttacagtgg
     2761 tgatttgact taaacatttt tcagtacctt tagctagtaa taatgtctag gtaaaggtac
     2821 taaacgatgc ttaaccagcc catgaggctg gtttttaacg tttaatttag tagaagagtg
     2881 atgaagagtg attaaagaag gtagtttgaa ataagaaaat ttttgttatg atcctaaaaa
     2941 gagtagatag tctttattgt taatttcggt gaagaaatga ttttaaaaag atatatcatc
     3001 gcattttcta atcatttagt gtaaaatagt aaggtaaaag tttagaagaa ttagtaatga
     3061 aaagataaaa aatgaacgaa aacattggat atggtaaggc acacagtaag atcattttga
     3121 taggtgagca tgctgttgtg tatggctacc cagctattgc tttgccttta acagatattg
     3181 aggtggtttg tcatattttt ccagctgata agccattggt ctttgatttt tatgatactc
     3241 tatcaacagc catttatgct gccttggatt atttgcagcg actacaagaa ccaattgctt
     3301 atgagattgt ttcacaagtg ccacaaaagc gtggtatggg gtcttcggca gccgtttcta
     3361 ttgcagctat tagagccgtt ttttcttatt gtcaagaacc cctctcggat gatttgttgg
     3421 aaattttagt caacaaggca gaaattattg cccacaccaa tcctagtggt ttagatgcca
     3481 agacatgcct tagtgaccat gccattaagt ttatccgaaa cattggcttt gaaactatcg
     3541 aaatcgcctt aaatggttat cttatcattg cagatacagg gattcacggt catacacgtg
     3601 aggccgtcaa taaggtagca cagtttgagg aaacgaattt gccctatctg gctaaactag
     3661 gagctttgac acaagccctt gaaagagcga ttaaccaaaa aaataaggtg gctatcggtc
     3721 agctcatgac acaagcgcac tctgctttaa aggccattgg cgttagcatc agtaaagctg
     3781 accaacttgt ggaggctgcc ctaagagcag gtgctttagg cgctaagatg acaggcggtg
     3841 gcttgggtgg ctgtatgatt gccctagcag ataccaaaga catggccgaa aaaatcagtc
     3901 acagattaaa agaagaagga gccgtaaaca cgtggatcca aatgttataa ccgtaacatc
     3961 ctatgctaac attgctatta tcaaatattg gggaaaagag aatcaagcta agatgattcc
     4021 ttcgacctct agcatttctt tgactttgga aaacatgttc accacaacca gcatttcctt
     4081 cttaccagat actgcaacca gtgatcagtt ttacattaac ggtgtcttgc aaaatgacga
     4141 agaacatacc aaaatttctg ctatcattga ccaatttcgc caacctggtc aggcttttgt
     4201 aaagatggaa actcaaaata atatgccaac agctgcaggt ttatcatcaa gttccagtgg
     4261 cttatcagcc ttggttaaag cctgtgacca actatttaac acccagctag atcagaaagc
     4321 tttagctcaa aaggctaagt tcgcctcagg atcatcttct cgttcttttt ttggcccagt
     4381 cgctgcttgg gacaaagata gtggtgctat ttacaaggtt gagactgact tgaaaatggc
     4441 catgattatg ctggtcttaa atgctgcgaa aaagccaatt tctagccgag agggcatgaa
     4501 gttatgccgc gatacctcaa ctacatttga tgagtgggta gaacaatcgg caatcgatta
     4561 ccaacatatg cttacctatc tcaaaactaa taattttgag aaagttggtc agttgacaga
     4621 agctaatgcc ttggccatgc atgccacgac aaagacagcc aatcctcctt tttcttacct
     4681 aacaaaagag tcttaccagg ctatggaggc tgtgaaagaa ctgcgtcaag aaggttttgc
     4741 ttgttatttt accatggacg caggtccaaa tgtgaaggtc ctatgtttag aaaaagactt
     4801 ggctcaactg gcagaacgac ttggcaagaa ctaccgtatt atcgtttcaa aaacaaagga
     4861 tttaccagat gtctaattat tgtgtgcaaa caggtgggaa actatacctc acaggcgaat
     4921 atgctatctt aacaccagga caaaaagcct taattcactt tattccactg atgatgacag
     4981 cagaaattag cccagcagcc catattcaat tagcttcaga tatgtttgcc cataaagcgg
     5041 gcatgacgcc cgatgcctct tatgcactga ttcaagcaac ggttaaaacc tttgctgact
     5101 atctaggaca gtcgattgac caactggagc cattttccct aatcataaca ggaaaaatgg
     5161 agcgcgatgg caaaaaattt ggcattggtt caagtggtag cgtcaccctc ttaaccttaa
     5221 aggccttatc ggcctattat cagatcactt taaccccaga gttactcttt aaactagcgg
     5281 cttatacctt gcttaagcaa ggagataatg ggtctatggg ggatattgcc tgtatcgctt
     5341 accagacttt agtcgcctat acctcctttg accgagaaca ggtcagtaac tggctgcaga
     5401 ccatgccctt aaaaaaactt ctcgtcaaag attggggtta ccatatccaa gtcattcaac
     5461 cagccctgcc ttgtgacttt ttggttggct ggactaaaat acctgctatt tcaaggcaga
     5521 tgattcaaca agtgacagcg agcattaccc cagctttctt aagaacaagt taccagctaa
     5581 cgcaatcagc tatggtagct ttgcaagaag gtcataagga agaactcaag aaaagtttag
     5641 caggagcaag tcatctccta aaagagcttc atccagctat ctaccatcct aagctagtaa
     5701 ccttggtagc tgcttgtcag aagcaagatg ctgttgctaa atcttcaggt tctggtggtg
     5761 gagattgtgg catcgcgctt gcctttaatc aggatgctag agataccctt atttccaaat
     5821 ggcaagaagc tgatatcgca ttactttatc aagaaaggtg gggagagaat gaccaaccgt
     5881 aaagatgatc acatcaaata tgccctcaag taccaatcgc tttataatgc ttttgatgac
     5941 atagaactca tacatcattc cttacctagc tatgatttgt ctgatattga tctcagtact
     6001 cattttgccg ggcaagactt cgactttccc ttttacatca atgccatgac aggaggaagt
     6061 caaaaaggca aagctgtcaa tgaaaaattg gccaaagtag cagcagcaac agggattgtc
     6121 atggtgacag ggtcttatag cgctgcttta aaaaatccta acgacgattc ctatcgttta
     6181 catgaggtgg cagataactt gaaactagcc acgaatattg gtctagataa acctgtggcg
     6241 ctaggacaac aaacggttca agaaatgcag cccctctttt tacaggttca tgtgaatgtg
     6301 atgcaagagt tgttgatgcc agagggtgag cgcgtctttc atacctggaa aaaacacctc
     6361 gctgaatacg ctagtcaaat accagttcct gtcattctca aagaagttgg ttttggcatg
     6421 gatgtcaata gtatcaagct agcacatgac ctaggcattc aaacctttga tatttcagga
     6481 agaggaggga cctcatttgc ctatattgaa aatcaaagag gaggagaccg ctcttactta
     6541 aacgattggg gacaaaccac cgttcagtgc ttgctaaatg cacaaggact gatggaccaa
     6601 gtggaaatct tagcttcggg tggtgtcaga caccccttgg acatgattaa atgtttcgtc
     6661 ctaggagcac gtgcagtggg actctcacgc accgttttag aattggtcga aaaataccca
     6721 accgagcgtg tgattgctat cgttaacggc tggaaagaag aattaaaaat cattatgtgt
     6781 gctcttgact gtaaaactat taaagaatta aaggaagtcg actacttact atatggacgc
     6841 ttgcagcagg tcaattaggc ttcatcacct ctacaatagt gccacctact acaaagatga
     6901 tagagctttt ttagaacata atataaaat
//
LOCUS       DAUDYX010000070         6841 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.071, whole genome shotgun sequence.
ACCESSION   DAUDYX010000070 DAUDYX010000000
VERSION     DAUDYX010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.071"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            34..4140
                     /gene="cas9"
                     /locus_tag="VHD02_001719"
     CDS             34..4140
                     /gene="cas9"
                     /locus_tag="VHD02_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922251.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEQ5498309.1"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGEIAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRLNSEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLAK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMLAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVRKDLIIKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PTAFKYFDTTIDRKRYTSTKEVLDATFIHQSITGLYETRIDLSQLGGD"
     gene            4140..5009
                     /gene="cas1"
                     /locus_tag="VHD02_001720"
     CDS             4140..5009
                     /gene="cas1"
                     /locus_tag="VHD02_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEQ5498310.1"
                     /translation="MAGWRTVVVNTHSKLSYKNNHLIFKDAYKTELIHLSEIDILLLE
                     TTDIVLSTMLVKRLVDENVLVIFCDDKRLPTAMLMPFYGRHDSSLQLGKQMSWSETVK
                     SQVWTTIIAQKILNQSCYLGACSYFEKSQSIMDLYHGLENFDPSNREGHAARIYFNTL
                     FGNDFSRDLEHPINAGLDYGYTLLLSMFAREVVVSGCMTQFGLKHANQFNQFNFASDI
                     MEPFRPLVDKIVYENRNQPFPKIKRELFTLFSDTFSYNGKEMYLTNIISDYTKKVVKA
                     LNNEGKGVPEFRI"
     gene            5006..5347
                     /gene="cas2"
                     /locus_tag="VHD02_001721"
     CDS             5006..5347
                     /gene="cas2"
                     /locus_tag="VHD02_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEQ5498311.1"
                     /translation="MSYRYMRMILMFDMPTDTAEERKAYRKFRKFLLSEGFIMHQFSI
                     YSKLLLNNTANNAMIGRLREHNPNKGNITLLTVTEKQFARMIYLHGERNNCIANSDER
                     LVFLGEAFDES"
     gene            5337..5999
                     /gene="csn2"
                     /locus_tag="VHD02_001722"
     CDS             5337..5999
                     /gene="csn2"
                     /locus_tag="VHD02_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HEQ5498312.1"
                     /translation="MNLNFSLLDEPIPLRGGTILVLEDVCVFSKIVQYCYKYEEDSEL
                     KFFDRKMKTIKESEIMLVTDILGFDVNSSTILKLIHADLESQFNEKPEVKSMIDKLVA
                     TITELIVFECLENELDLEYDEITILELIKSLGVKVETQSDTIFEKCLEILQIFKYLTK
                     KKLLIFVNSGAFLTKDEVASLQEYISLTNLTVLFLEPRELYDFPQYILDEDYFLITKN
                     MV"
     gene            6383..6841
                     /locus_tag="VHD02_001723"
                     /pseudo
     CDS             6383..6841
                     /locus_tag="VHD02_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051153.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
BASE COUNT         2319 a         1017 c         1388 g         2117 t
ORIGIN      
        1 tttaatttaa actttttatt ttaggaggca aaaatggata agaaatactc aataggctta
       61 gatatcggca caaatagcgt cggatgggcg gtgatcactg atgattataa ggttccgtct
      121 aaaaagttca aggttctggg aaatacagac cgccacagta tcaaaaaaaa tcttataggg
      181 gctcttttat ttgacagtgg agagatagcg gaagcgactc gtctcaaacg gacagctcgt
      241 agaaggtata cacgtcggaa gaatcgtatt tgttatctac aggagatttt ttcaaatgag
      301 atggcgaaag tagatgatag tttctttcat cgacttgaag agtctttttt ggtggaagaa
      361 gacaagaagc atgaacgtca tcctattttt ggaaatatag tagatgaagt tgcttatcat
      421 gagaaatatc caactatcta tcatctgcga aaaaaattgg tagattctac tgataaagcg
      481 gatttgcgct taatctattt ggccttagcg catatgatta agtttcgtgg tcattttttg
      541 attgagggag atttaaatcc tgataatagt gatgtggaca aactatttat ccagttggta
      601 caaacctaca atcaattatt tgaagaaaac cctattaacg caagtggagt agatgctaaa
      661 gcgattcttt ctgcacgatt gagtaaatca agacgattag aaaatctcat tgctcagctc
      721 cccggtgaga agaaaaatgg cttatttggg aatctcattg ctttgtcatt gggtttgacc
      781 cctaatttta aatcaaattt tgatttggca gaagatgcta aattacagct ttcaaaagat
      841 acttacgatg atgatttaga taatttattg gcgcaaattg gagatcaata tgctgatttg
      901 tttttggcag ctaagaattt atcagatgct attttacttt cagatatcct aagattaaat
      961 agtgaaataa ctaaggctcc cctatcagct tcaatgatta aacgctacga tgaacatcat
     1021 caagacttga ctcttttaaa agctttagtt cgacaacaac ttccagaaaa gtataaagaa
     1081 atcttttttg atcaatcaaa aaacggatat gcaggttata ttgatggggg agctagccaa
     1141 gaagaatttt ataaatttat caaaccaatt ttagaaaaaa tggatggtac tgaggaatta
     1201 ttggcgaaac taaatcgtga agatttgctg cgcaagcaac ggacctttga caacggctct
     1261 attccccatc aaattcactt gggtgagctg catgctattc tgagaagaca agaagacttt
     1321 tatccatttt taaaagacaa tcgtgagaag attgaaaaaa tcttgacttt tcgaattcct
     1381 tattatgttg gtccattggc gcgtggcaat agtcgttttg catggatgac tcggaagtct
     1441 gaagaaacaa ttaccccatg gaattttgaa gaagttgtcg ataaaggtgc ttcagctcaa
     1501 tcatttattg aacgcatgac aaactttgat aaaaatcttc caaatgaaaa agtactacca
     1561 aaacatagtt tgctttatga gtattttacg gtttataacg aattgacaaa ggtcaaatat
     1621 gttactgagg gaatgcgaaa accagcattt ctttcaggtg aacagaagaa agccattgtt
     1681 gatttactct tcaaaacaaa tcgaaaagta accgttaagc aattaaaaga agattatttc
     1741 aaaaaaatag aatgttttga tagtgttgaa atttcaggag ttgaagatag atttaatgct
     1801 tcattaggta cctaccatga tttgctaaaa attattaaag ataaagattt tttggataat
     1861 gaagaaaatg aagatatctt agaggatatt gttttaacat tgaccttatt tgaagatagg
     1921 gagatgattg aggaaagact taaaacatat gctcacctct ttgatgataa ggtgatgaaa
     1981 cagcttaaac gtcgccgtta tactggttgg ggacgtttgt ctcgaaaatt gattaatggt
     2041 attagggata agcaatctgg caaaacaata ttagattttt tgaaatcaga tggttttgcc
     2101 aatcgcaatt ttatgcagct gatccatgat gatagtttga catttaaaga agacattcaa
     2161 aaagcacaag tgtctggaca aggcgatagt ttacatgaac atattgcaaa tttagctggt
     2221 agccctgcta ttaaaaaagg tattttacag actgtaaaag ttgttgatga attggtcaaa
     2281 gtaatggggc ggcataagcc agaaaatatc gttattgaaa tggcacgtga aaatcagaca
     2341 actcaaaagg gccagaaaaa ttcgcgtgag cgtatgaaac gtattgaaga aggtatcaaa
     2401 gaattaggaa gtcagattct taaagagcat cctgttgaaa atactcaatt gcaaaatgaa
     2461 aagctctatc tctattatct ccaaaatgga agagacatgt atgtggacca agaattagat
     2521 attaatcgtt taagtgatta tgatgtcgat cacattgttc cacaaagttt cattaaagac
     2581 gattcaatag acaataaggt cttaacgcgt tctgataaaa atcgtggtaa atcggataac
     2641 gttccaagtg aagaagtagt caaaaagatg aaaaactatt ggagacaact tctaaacgcc
     2701 aagttaatca ctcaacgtaa gtttgataat ttaacgaaag ctgaacgtgg aggtttgagt
     2761 gaacttgata aagctggttt tatcaaacgc caattggttg aaactcgcca aatcactaag
     2821 catgtggcac aaattttgga tagtcgcatg aatactaaat acgatgaaaa tgataaactt
     2881 attcgagagg ttaaagtgat taccttaaaa tctaaattag tttctgactt ccgaaaagat
     2941 ttccaattct ataaagtacg tgagattaac aattaccatc atgcccatga tgcgtatctt
     3001 aatgccgtcg ttggaactgc tttgattaag aaatatccaa aacttgaatc ggagtttgtc
     3061 tatggtgatt ataaagttta tgatgttcgt aaaatgcttg ctaagtctga gcaggaaata
     3121 ggcaaagcaa ccgcaaaata tttcttttac tctaatatca tgaacttctt caaaacagaa
     3181 attacacttg caaatggaga gattcgcaaa cgccctctaa tcgaaactaa tggggaaact
     3241 ggagaaattg tctgggataa agggcgagat tttgccacag tgcgcaaagt attgtccatg
     3301 ccccaagtca atattgtcaa gaaaacagaa gtacagacag gcggattctc caaggagtca
     3361 attttaccaa aaagaaattc ggacaagctt attgctcgta aaaaagactg ggatccaaaa
     3421 aaatatggtg gttttgatag tccaacggta gcttattcag tcctagtggt tgctaaggtg
     3481 gaaaaaggga aatcgaagaa gttaaaatcc gttaaagagt tactagggat cacaattatg
     3541 gaaagaagtt cctttgaaaa aaatccgatt gactttttag aagctaaagg atataaggaa
     3601 gttagaaaag acttaatcat taaactacct aaatatagtc tttttgagtt agaaaacggt
     3661 cgtaaacgga tgctggctag tgccggagaa ttacaaaaag gaaatgagct ggctctgcca
     3721 agcaaatatg tgaatttttt atatttagct agtcattatg aaaagttgaa gggtagtcca
     3781 gaagataacg aacaaaaaca attgtttgtg gagcagcata agcattattt agatgagatt
     3841 attgagcaaa tcagtgaatt ttctaagcgt gttattttag cagatgccaa tttagataaa
     3901 gttcttagtg catataacaa acatagagac aaaccaatac gtgaacaagc agaaaatatt
     3961 attcatttat ttacgttgac gaatcttgga gctcccactg cttttaaata ttttgataca
     4021 acaattgatc gtaaacgata tacgtctaca aaagaagttt tagatgccac ttttatccat
     4081 caatccatca ctggtcttta tgaaacacgc attgatttga gtcagctagg aggtgactga
     4141 tggctggttg gcgtactgtt gtggtaaata cccactcgaa attatcctat aagaataatc
     4201 atctgatttt taaggatgcc tataaaacgg agctgatcca tttatcagaa attgatattt
     4261 tgttattaga aacgaccgat attgtcttgt ccactatgct ggtaaaacgg ctagtggatg
     4321 agaatgtcct tgtcatattc tgtgatgata aacgattacc aacagctatg ctgatgcctt
     4381 tttatggtcg tcatgattcg agtttacagc ttgggaaaca aatgtcctgg tcagaaacag
     4441 tcaaatcgca ggtttggacg acgattattg ctcaaaagat tttgaatcaa tcttgctatc
     4501 taggagcatg ctcctatttt gaaaaatccc aatctattat ggatttatat catggtttgg
     4561 aaaattttga tccgagtaat cgagaagggc atgcagcgag aatttatttt aatacacttt
     4621 ttgggaacga tttctcaaga gatttggagc atccaatcaa tgcaggtctg gattatggtt
     4681 atactttatt attgagtatg tttgcgcgtg aagtggttgt gtctggatgt atgactcagt
     4741 ttgggcttaa acacgctaat cagtttaatc agttcaattt tgctagcgat attatggaac
     4801 catttaggcc tttggtggat aagattgttt atgaaaatcg aaatcagcct tttcccaaaa
     4861 taaagagaga gttatttact ttgttttcag atacattttc atataatggt aaagagatgt
     4921 atctcacgaa tattattagc gattatacta aaaaagttgt caaagctctg aataatgaag
     4981 ggaaaggagt tcctgaattt aggatatgag ttatagatat atgagaatga tacttatgtt
     5041 tgatatgccg acggacaccg ctgaggaacg aaaagcctat cgaaaatttc ggaaattttt
     5101 acttagtgaa gggtttatca tgcatcaatt ttctatttat agtaagttgc tgttgaataa
     5161 tacagctaac aatgccatga ttggtcggct gagggagcat aatcctaata aaggaaatat
     5221 tacattacta acggtcacag aaaaacagtt tgcacgaatg atttatttac atggtgaaag
     5281 aaataattgt attgcaaact ccgatgaaag acttgtattt cttggggagg cttttgatga
     5341 atcttaattt ttccttacta gatgaaccga ttccattaag aggcggtaca attcttgtgc
     5401 tcgaagatgt ctgtgtattt tcaaaaatag tgcaatattg ttacaaatat gaggaagatt
     5461 ctgaacttaa attttttgat cgcaagatga aaaccatcaa agaatcagaa atcatgcttg
     5521 taacagatat tttaggattt gatgttaact cctcaaccat tttaaaattg attcatgcag
     5581 atttagaatc tcaatttaat gagaaacccg aagtgaaatc gatgattgac aaattggttg
     5641 ctacgattac agaactgatt gtctttgaat gcttagaaaa tgaattagat ttagagtatg
     5701 atgaaatcac aatcctggaa ttgattaagt ccttaggagt aaaagtagaa acgcaaagtg
     5761 atactatttt tgaaaaatgt ctagagatac ttcaaatttt caaatatctc actaagaaaa
     5821 agttgcttat ttttgtcaat agcggagctt ttctaacaaa ggatgaagtg gctagtttac
     5881 aagagtatat atcattgaca aatttaacag ttctcttttt agaaccacgt gaactatatg
     5941 attttccgca gtatatttta gatgaagatt atttcttaat aactaaaaat atggtataat
     6001 actcttaata aatgcagtaa tacaggggct tttcaagact gaagtctagc tgagacaaat
     6061 agtgcgatta cgaaattttt tagacaaaaa tagtctacga ggttttagag ctatgctgtt
     6121 ttgaatggtc ccaaaacgat tgaccacaac atccaacgct taggttatgt tttagagcta
     6181 tgctgttttg aatggtccca aaacaataga gctagacaaa aaattgagtt tgacgtttta
     6241 gagctatgct gttttgaatg gtctccattc aacattgccg atgataactt gagaaagagg
     6301 gttaatacca gcagtcggat acctttctat tctttctgtt aaagcgtttt catgttataa
     6361 taggcaaaag aagagtagtg tgatggaaca aacatttttt atgattaagc cagatggggt
     6421 taagcaaggg gaggtagttg gagaggtttt gcggtggatt gaacgcctaa gatttacgtt
     6481 taagcgattc gagctaagac aagctagttc gaaatacttg gctaagcacg acgaggcctt
     6541 ggtgataaac cttttgatcc taaacttaaa gcttacatga caagtggtcc tgttttaatt
     6601 gagacacttc ttggggacta aggtggtttt gtctttttga accatgatga gagtaaccaa
     6661 tcctacggat gctctttgcg gaaccattcg tgggaatttt gctcaggctc ttagtgatga
     6721 ttgggaaatt tttagtatgg tgcatgggtc tcatttaagc gactctgcta ggcgtgagat
     6781 tgtcctttgg tctcatcaaa gtaatctcgg tagctccgct attctcttgc aagataatcc
     6841 g
//
LOCUS       DAUDYX010000071         6781 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.072, whole genome shotgun sequence.
ACCESSION   DAUDYX010000071 DAUDYX010000000
VERSION     DAUDYX010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6781
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.072"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            89..862
                     /gene="cfa"
                     /locus_tag="VHD02_001724"
     CDS             89..862
                     /gene="cfa"
                     /locus_tag="VHD02_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_149017463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfa"
                     /protein_id="HEQ5498313.1"
                     /translation="MKPKHLLCLSTVVAGLALFSTMTHSVLADDASNPDTILMNNNQA
                     NLQRDALVQKLDEGHQQLEAIKHEAKGTDIEATVNKAIDAVDHMKSSIRFNTETIYDF
                     SSIGARVEALSDAIKAIVFSTTQLTHKVEKAHTDMGFAITKLVIRIIDPFASVDAIKA
                     QVQEIKALEEKVINYPDLQPTDRATIYTKAKLNKAIWNTRLERDKKVLRIKPFDVYNR
                     LNKAITHAVGVQLNPTTTVQQVDDEVIAVQDALETALKS"
     gene            complement(1232..2467)
                     /locus_tag="VHD02_001725"
     CDS             complement(1232..2467)
                     /locus_tag="VHD02_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEQ5498314.1"
                     /translation="MTQDPIANLKLARKGPIVSIIVYLLLSVAKLLAGYLLNASSLIA
                     DGFNNLSDIVGNVALLIGLHLASQPADANHKFGHWKIEDLSSLVTSFIMFLVGFQVLI
                     HTIKSIFSGQQVDIDPLGAIVGIVSAFVMLGVYVFNKRLSKRVKSSALVAASKDNLAD
                     AVTSIGTSIAIIAASLHLPVIDHIAAMIITFFILKTAFDIFMESSFSLSDGFDSRHLK
                     KYEKAILEIPKIVSVKSQRARTYGSNVYLDIVLEMNPDLSVYESHSITEKVEQLLSDQ
                     FSIYDIDIHVEPAMIPEEEIFDNVAKKLYRYEKLILSKVPDYDHYIAKSFQLIDANGQ
                     TVNYEQFLNQEIYYPSNFNHFQIESISQKTMLVTYQLNGNQRTSIWRCHESWSLLFHQ
                     ITPIAKKQLHHTHYRIVKM"
     gene            complement(2587..3909)
                     /locus_tag="VHD02_001726"
     CDS             complement(2587..3909)
                     /locus_tag="VHD02_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEQ5498315.1"
                     /translation="MIALVKLIDNLVWGPPLLILLVGTGIYLTSRLGLIQILKLPRAF
                     KLIFSDDEGHGDISSFAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFLGM
                     ATKYAEGVLAIKYRTKDANGHISGGPMYYIVNGMGTKWKPLAILFAGSGILVALFGIG
                     TFAQVNSITSSLGHSFGLSPQMVSIVLAIFVAAIIFGGIHSISKVAEKVVPFMAIFYI
                     LSSLAVIFSHYQQLLPVIRLVFQSAFTPTAAIGGFAGSLMKDAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTIIICTLTGLSILVTGQWTGQLEGAPLT
                     QSAFATVFGNLGTFGLTFSLVLFAFTTILGWSYYGERCFEFLFGITHLTYFRIVFILM
                     VGLGGFLKLELIWVLADIVNGLMALPNLIALLALSPVVILETKHYFIK"
     regulatory      complement(4037..4123)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            4438..6756
                     /gene="pcrA"
                     /locus_tag="VHD02_001727"
     CDS             4438..6756
                     /gene="pcrA"
                     /locus_tag="VHD02_001727"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEQ5498316.1"
                     /translation="MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKFVNPWNILAITFTNKAAREMKERALALNPATKDTLIATFHSMCVRILRREADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQA
                     ADMYSQIVARCYKAYQEELRRSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLIKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVL
                     LEENYRSTKKILQAANDVINNNRNRRPKKLWTQNADGEQLVYYRANDERDEAVFVAST
                     ISNMSQELGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDLIA
                     YLTIVANPADNISFERIVNEPKRGVGPGTLDKLRQFAYESDQSLLEAASNLLMSPLKG
                     KAAQAIMDLANILGQLRQDLDQMSITDLAEALLEKTGYLDSLRLQNTLESQARIENIE
                     EFLSVTKNFDESSASQEEDETGVDRLGRFLNDLALIADTDDSQAEAAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRASEDPDELEEERRLAYVGITRAEEVLFMTNANTRTLF
                     GKSSYNRPTRFLKEISEELLSYKGLARPAQSSFGVRFSTETHTQFGQGMSLSEALQAR
                     KAQAQVRQSAQPMQAHTIPSASTSSVLPFGSNSSVEEVTWQIGDIAHHKKWGDGTVLE
                     VSGSGKTMELKIKFPEVGLKKLLASVAPIEKK"
BASE COUNT         2267 a         1273 c         1337 g         1904 t
ORIGIN      
        1 agtagtatga taacgttata agcgcttata aattaattta acttcactta actatttaaa
       61 taggtttatt atattcagga ggaataatat gaaacccaaa caccttctct gtctttcaac
      121 agttgttgct ggattagctt tattttcaac tatgacgcac tcagttttag ctgatgatgc
      181 ttcaaatccc gatactattt taatgaataa caaccaagcg aatcttcaaa gagatgctct
      241 agtgcaaaag cttgatgagg gacatcaaca attagaagct attaaacatg aagctaaagg
      301 tactgatatt gaggctactg ttaacaaagc tatcgatgct gttgatcaca tgaagagttc
      361 tatacgcttc aatactgaaa cgatctatga ttttagttca attggggcaa gagtagaagc
      421 attatcagat gctatcaaag caatcgtatt ttccacaacc caattaactc ataaagtaga
      481 aaaagcgcat actgatatgg ggtttgctat tactaaatta gtcattcgta ttatagatcc
      541 atttgcatct gttgacgcta tcaaagctca agtacaagaa attaaagcac ttgaggaaaa
      601 agttatcaat tatcctgatt tacagccaac agaccgtgct actatctata ctaaagctaa
      661 actaaataaa gctatttgga acactcgttt agaaagagat aaaaaggttt tgaggatcaa
      721 accatttgat gtttacaata gacttaacaa agctatcaca catgcggttg gtgtccaact
      781 aaatccgaca accactgttc aacaagttga cgatgaagtt attgctgtgc aagatgcttt
      841 ggaaacagct cttaagtcat agagtgtata agtaatgtgt tgtataggta gatgattata
      901 ttaggatata gaataggtaa agattttaaa tatcttacca ttgtagagtt tgaggtgatg
      961 ctctcataaa caatgagtta cctgtagccc tttttgcctt taacatgatg taccaattta
     1021 aggtattgtt caaccgtcac tattgaatct tgtttaacct cctaaactaa taagattcaa
     1081 tatgaaaacg tgtattctta aaaaaatccc tatgatctgt ttaaaaacaa attgtaggga
     1141 tttttttaac tatttcactt atcattttgg gactaggaat cgctttagga atatcatgaa
     1201 gtatagttat gttataagag aagtaatgtt tttacatttt tacaatacgg tagtgtgtgt
     1261 gatgtaattg tttcttagcg ataggggtaa tttggtggaa gagtaaggac caagattcat
     1321 gacacctcca aatactggta cgttgattgc catttaattg gtaagttacc aacatcgttt
     1381 tttgactaat ggattcaatc tgaaaatggt tgaagttact tggataataa atttcttggt
     1441 tcaaaaattg ttcatagtta actgtttggc cattcgcatc aatcagttgg aaagacttag
     1501 caatgtagtg gtcatagtca ggaaccttac tcaaaattaa tttttcgtag cggtagagct
     1561 ttttggcgac attatcaaaa atctcttctt cgggaatcat ggcaggctca acgtgaatgt
     1621 caatgtcata aatagaaaat tggtcactca ataactgctc cactttctct gtaatagagt
     1681 gactttcata gactgaaaga tcaggattca tttcaagtac aatatcaaga tagacattgc
     1741 taccataggt cctagctcgt tgagacttaa cggagacaat tttagggatt tctaaaatgg
     1801 ctttttcgta ttttttcaaa tgacggctat caaatccatc agataaacta aacgaacttt
     1861 ccataaagat atcaaaagct gttttaagaa taaagaacgt aatgatcata gcagctatat
     1921 gatcgataac tggtaaatgc aaagaagctg ctataatagc aattgatgtt ccgatagaag
     1981 taacagcatc agctagatta tccttagaag cagcgactaa ggcactggat tttacacgtt
     2041 tggaaagacg tttgttaaag acataaaccc ctaacataac aaaagctgaa acgataccga
     2101 caatagcccc aagagggtca atatcaactt gctggccact aaagatactt ttaattgtgt
     2161 gaatcagtac ttggaaacct acaagaaaca taataaaaga agtgacaagg ctggataagt
     2221 cttcaatttt ccaatgacca aatttatgat tggcatcggc tggttggcta gctaagtgaa
     2281 gaccaataag cagggctaca tttcccacaa tatccgataa attgttaaat ccatcagcaa
     2341 taagggaact tgcatttaac aaataaccag ctaataattt ggcaacacta agcaacaagt
     2401 agacaataat actaacgatt gggccctttc tggctagttt taaatttgca atcggatcct
     2461 gtgtcataat aactcctttg atttcaactt tatattatac cataaaaagg ggattaagaa
     2521 aaaagcctag cttcttgaac ttggcagaag aagactaggt ataactgggt agtactaacg
     2581 tcatgtttac ttaataaagt aatgcttggt ttctaaaata acaactggcg agagagctag
     2641 gagggcaatc aggttaggaa gtgccattaa accattgaca atatcagcca aaacccaaat
     2701 cagttctaac ttaaggaagc ctcctaggcc taccattaag ataaagacaa tacggaagta
     2761 ggtgagatgt gtaatgccaa agaggaattc aaagcagcgt tctccataat aactccatcc
     2821 taaaatcgtt gtaaaggcga agagaactag ggaaaaggtt aggccaaaag tccctaaatt
     2881 tccaaacaca gtggcaaagg cagattgggt cagaggagcc ccttcgagct gcccagtcca
     2941 ttggcctgtt actaaaatag ataggccggt tagggtacag ataataatcg tatcaataaa
     3001 agttcctgtc atggagatca gtccctgctc cacgggttca ttggttttag ctgctgctgc
     3061 agcaataggt gcagatccta gtccagactc attggagaag acaccgcgtg caattccttt
     3121 ttgaatggca tccttcatga gacttccagc aaagcctcca atagcagctg tcggagtgaa
     3181 ggctgattga aagactaaac gaataacagg aagtagctgt tggtagtgac taaaaataac
     3241 tgctaaacta gatagaatat aaaaaatagc cataaaagga acgacttttt cagccacttt
     3301 ggaaatggaa tgaatgccac caaaaataat ggcagccaca aagatagcta aaacaatact
     3361 gaccatttgt ggggataagc caaagctatg acctaaagac gaggtgatcg agttcacttg
     3421 agcaaaggtt cctattccaa atagggcaac taggatccct gaacccgcaa aaagaatggc
     3481 taagggtttc cattttgtcc ccatgccatt aacaatatag tacataggac caccagaaat
     3541 atgaccgttg gcatcctttg tacggtattt aatcgctagt accccttcag cgtacttagt
     3601 ggccattccc aaaaaagcgg caacccacat ccaaaagagc gctccaggac caccagactt
     3661 gatagcagtg gcaaccccaa caatgttacc agttccgaca gtagcggcaa gggcagttgc
     3721 aagagcagca aaggatgaaa tatctccatg tccttcgtca tctgaaaaaa tgagtttaaa
     3781 ggctcttggt agttttaaga tttgaattaa tcctaaacga ctggtaaggt aaatccccgt
     3841 cccaaccaat aaaattaaga ggggcggccc ccaaacaagg ttatcaatta atttaacgag
     3901 tgctatcatc tttctctcct aaaagtcatc gaagacgcca aaagaaaaaa gctatctctt
     3961 tctttgagac aacttctgaa gcaatgataa aacagcaaac acaaaaaaga ctaagactgt
     4021 tcttaatggt gtttgtattt taccttctgt ccttttgcct gagagattga gcagtatgcc
     4081 ttgccccttc ggcgcctaat aaggtctctc cagaagtccg tccatcaaac agtcccgttc
     4141 aaaaactgaa cacctgagag tataactcct tcggcgaaaa aaattcttct cctgcatgac
     4201 atcattcggt ttattaaatt aggactatca taccttaaaa aacgaatgaa gtcaaccaaa
     4261 atagcaaaaa aataaggaat gctttaagaa atcgctatta tttttaaata tctgatccta
     4321 aactagaaaa aaggaatctc tttacttttg gggtcttctt tgctattttt cttggaaaca
     4381 tgatataatt ttaaggttga taaaagagat gtcactgaac gagattaagg agaaagcatg
     4441 aatcctttat taaatggaat gaatgatcgg caggcacaag ctgttcaaac aactgaaggc
     4501 cccttattga ttatggcggg ggctggttct ggtaaaacaa gggtactgac ccatcgtatt
     4561 gcctatttaa ttgatgaaaa atttgtcaat ccttggaata ttttagctat tacctttact
     4621 aataaggctg cgcgtgaaat gaaagaaagg gccttggcct taaatccagc tactaaagat
     4681 accttaattg ctacctttca ctccatgtgt gtacggattc tgcgtcgtga ggctgatcac
     4741 attggttaca accgaaactt cacaatcgtg gatccaggtg agcaacgaac gttgatgaag
     4801 cgcattttaa aacaattaaa tatagaccct aaaaagtgga atgaacgttc tattttagga
     4861 actatttcaa acgctaaaaa tgatttgctt gatgaaaagg gatacgaggc acaggctgct
     4921 gatatgtata gtcaaatcgt agcgaggtgt tacaaagctt atcaggaaga attacgacgt
     4981 agcgaagcac ttgactttga tgatttaatc atgatgacgc tacgcctgtt tgacgctaat
     5041 cctgacgtgt tagcttatta ccagcagcgt taccaataca ttcatgtgga tgagtaccaa
     5101 gataccaacc atgctcaata tcagctaatt aaattactag cttctcgctt taaaaatatt
     5161 tgcgttgttg gagacgctga tcagtctatc tatggttggc ggggagctga catgcaaaat
     5221 attcttgatt ttgaaaaaga ttacccagat gctaaggtag tgcttttaga agaaaactat
     5281 cgctctacca agaaaattct gcaagcagct aacgacgtga ttaacaataa ccgtaatcgc
     5341 cgtcctaaga aactttggac acaaaatgcg gatggggaac aactggttta ttaccgggca
     5401 aatgatgagc gagatgaggc agtatttgtg gcctcaacca ttagcaatat gagtcaagag
     5461 cttggaaaaa acttcaaaga tttcgctgtt ttataccgta ccaatgccca atctcggacc
     5521 attgaagaag cactcttaaa atccaatatt ccttacacca tggttggagg caccaagttt
     5581 tacagccgta aagaaattcg ggatttgatt gcctatttaa ccattgttgc taaccctgct
     5641 gataatattt cttttgagcg gattgtgaat gagcctaaac gaggtgttgg tccaggaacg
     5701 cttgataaac tgcgtcagtt tgcctatgag agtgaccagt ctttattaga agcagcttca
     5761 aatctattga tgtccccctt gaaaggtaaa gcagcacaag ctatcatgga cttggccaat
     5821 attctcggtc agctacgtca agacctagat cagatgagta tcaccgactt agcagaagct
     5881 ttgttagaaa aaacaggcta tttagatagc ttacgcctgc aaaatacatt ggaaagccaa
     5941 gcccgtatcg aaaatatcga agaattctta tctgttacca aaaattttga cgagtcaagt
     6001 gcctctcaag aagaagacga aaccggtgtt gatcgcttgg gacgtttttt aaacgattta
     6061 gcactgattg ctgatacaga tgattcccaa gcagaagctg ctgaagtaac cttaatgacc
     6121 ctacatgctg ccaaaggctt ggagttccct gttgttttct taattggtat ggaagaaggt
     6181 gtcttcccct tatcacgtgc ctctgaagat ccagatgaat tggaagaaga aagacgttta
     6241 gcttatgtcg gaatcacaag agctgaagaa gttctcttta tgaccaatgc caacaccaga
     6301 accctctttg gtaaaagtag ttataaccgt ccaacacgct tcttgaaaga aatttcagag
     6361 gaactactct cttataaagg attggcaaga ccagcacaat cttcatttgg tgtgcgtttt
     6421 tctactgaaa cacacacaca gtttggacaa gggatgagcc tatcagaagc cttacaagct
     6481 cgaaaggccc aggctcaagt aagacaatca gcacagccaa tgcaggcaca tacgatacca
     6541 tcagcttcaa ctagctcagt tttaccgttt ggttctaata gctctgtaga agaggtcact
     6601 tggcagattg gcgatatcgc tcaccataaa aaatggggag acgggactgt gcttgaggtt
     6661 tcaggctccg gaaaaacgat ggaattgaaa atcaaatttc cagaagttgg tttaaagaaa
     6721 ttactagcaa gtgttgctcc tattgagaaa aaataagaaa aaaatgagct tcctactata
     6781 t
//
LOCUS       DAUDYX010000072         6593 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.073, whole genome shotgun sequence.
ACCESSION   DAUDYX010000072 DAUDYX010000000
VERSION     DAUDYX010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.073"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..968
                     /locus_tag="VHD02_001728"
     CDS             <1..968
                     /locus_tag="VHD02_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEQ5498317.1"
                     /translation="SHLRSVNTLTISKIVYQKKFFHLAKKSEKVISTYHVTDDLKPFQ
                     VKDLVSGHLERIQEEVEKKYPNAGFNSDKYNGLKESNSLLSDGFEVKSGNLIFDKKLT
                     IPLTTLFDVINPDFLANSDRAAYDNYRTYKEQHPKKLVALTFDDGPDPTTTPQVLDIL
                     AKYQAKGTFFMIGSKVVNNENLTKRVSDAGHEIANHTWDHPNLTNLSVSEIQHQVNMT
                     NQAIEKACGKKPRYLRPPYGATNATVQQSSGLTQMLWTVDTRDWENHSTDGIMTNVKN
                     QLQPGGVVLMHDIHQTTINALPTVMEYLKAEGYECVTVSELYAHQ"
     gene            complement(1079..2164)
                     /locus_tag="VHD02_001729"
     CDS             complement(1079..2164)
                     /locus_tag="VHD02_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ5498318.1"
                     /translation="MITKFPPQWQEKLDQVAFTHLTPIQEQAFQPIVDGKNFLGISPT
                     GTGKTLAYVFPSLLALTPKKSQQLLILAPNTELAGQIFEVTKDWAQPLGLTAQLFISG
                     TSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMSVNTIVLDEYDELLGDSQYDFV
                     QKISHYVPRDHQMVYMSATNKVDQTSLAPNTFCIDLSEQTNDAIQHFYLMVDKRERTD
                     LLRKFANIPHFRALVFFNSLSDLGATEERLQYNGAAAVSLASDINVKFRKTILEKFKS
                     HQLSLLLATDLVARGIDIDNLDYVIHFDVARDKENYTHRAGRTGRMGKSGIVITFVSH
                     PEDLKKLKKFAKVSEISLKNQQLHFIN"
     gene            2262..2888
                     /gene="udk"
                     /locus_tag="VHD02_001730"
     CDS             2262..2888
                     /gene="udk"
                     /locus_tag="VHD02_001730"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEQ5498319.1"
                     /translation="MLKKPIIIGVTGGSGGGKTSVSRAILDSFPNARIAMIQHDSYYK
                     DQSHMSFEERVKTNYDHPLAFDTDFMIQQLKELLAGRPVDIPIYDYKKHTRSNTTFRQ
                     DPQDVIIVEGILVLEDERLRDLMDIKLFVDTDDDIRIIRRIKRDMMERGRSLESIIDQ
                     YTSVVKPMYHQFIEPSKRYADIVIPEGVSNVVAIDVINSKIASILGEV"
     gene            2968..3231
                     /locus_tag="VHD02_001731"
     CDS             2968..3231
                     /locus_tag="VHD02_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEQ5498320.1"
                     /translation="MLLIWLGLLFVWNELVFVIYAFDKRKAIKKKRRISERNLLVITV
                     LFGGFGALLAAKKYHHKTRKWYFVITWYTSILLTLLVTYIFMK"
     gene            complement(3284..4048)
                     /locus_tag="VHD02_001732"
     CDS             complement(3284..4048)
                     /locus_tag="VHD02_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEQ5498321.1"
                     /translation="MALVLNGIELELLGLTANNLSFKEAFALILTISLLGIYLIPFAA
                     AIFYLSKKFHMNLNVIIVSCLSGLYISGFLASCGNHLVGQFWSYIIPSKDALKLWGDA
                     LTAPIVEEPIKASSAILVITLFPRLTLKEKLVVALLSGMGFQLTEDIRYLIQAKSIDS
                     LVPTAIERISTAVTSHWVHTAIFTIGAYLLLKGSNLFSKQQQIFWLLSPLVLHFIWNS
                     PLTSIPGMTVLLGTLILLIFGDLFQKINTLDDDVLF"
     gene            4238..4735
                     /locus_tag="VHD02_001733"
     CDS             4238..4735
                     /locus_tag="VHD02_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEQ5498322.1"
                     /translation="MNKSKKIEQYQLMIEQAKELFANESNALANLSNASALLNMTLPN
                     SVFAGFYLFDGQELILGPFQGRVSCVHIKLGKGVCGESAQSRRTIIINDVKQHANYIS
                     CDAAAMTEIVVPMVKEGHLIGVLDLDSSLVADYDEVDQEYLEAFVDLFLEKTTFTFNM
                     FGVKN"
     gene            4735..6420
                     /gene="dnaX"
                     /locus_tag="VHD02_001734"
     CDS             4735..6420
                     /gene="dnaX"
                     /locus_tag="VHD02_001734"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEQ5498323.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVESGKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVDGEPCNQCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKQKAIREHLAWVLDKEGLAYEVDALNLIARRAEGGMRDALS
                     ILDQALSLSPDNQVTIAIAEEITGSISILALGDYVRYVSQEQATQALAALETIYDSGK
                     SMSRFATDLLTYLRDLLVVKAGGDNQRQSAVFDTNLSLSIDRIFQMITVVTSHLPEIK
                     KGTHPRIYAEMMTIQLAKEEGVALPSGLSSDVTTEVEGLKRELNQLKQALAQLQASPS
                     PSHAPVKPAQAKPKLTTYKIDRVKILKIMEETVQDSQQSRQYLDALKTAWNEILDSIS
                     AQDRALLMGSEPVLANSENAILAFEAAFNAEQAMKRTDLNAIFGNIMSKAAGFSPNIL
                     AVPKADFHHIRSEFALQLKTQKASDPTEETIEETLEIPEGFDFLVDKINAVED"
BASE COUNT         2136 a         1115 c         1338 g         2004 t
ORIGIN      
        1 cttctcacct aagatcagtt aacactttga ctatttctaa aatagtttat cagaaaaaat
       61 tttttcattt ggctaaaaaa tcagaaaaag ttataagtac atatcacgtt acagacgatt
      121 tgaaaccgtt tcaggtaaag gatctagtat caggacattt agaaagaata caagaagaag
      181 ttgaaaaaaa atatccaaat gctggtttta atagcgataa gtacaatggc ttaaaagaat
      241 ctaattcttt attaagcgat ggctttgagg taaaatcggg aaaccttatt tttgataaaa
      301 agctaacgat acctttgacg acattatttg atgttattaa tccagatttt ttagcaaata
      361 gcgatagagc tgcgtatgat aattatagga cctacaaaga acagcatccc aaaaaactag
      421 ttgcattaac gtttgatgat ggtccagatc cgacgacgac tcctcaagtt ttagatattt
      481 tggcaaaata ccaggctaag ggaactttct ttatgatagg ttcaaaagtt gtgaataatg
      541 aaaaccttac taaacgtgtt agcgacgctg gccatgaaat tgctaatcat acttgggatc
      601 atcccaatct gactaatctt tcagtcagcg agattcaaca tcaagttaat atgacgaacc
      661 aggctattga aaaagcttgt ggcaagaaac ctcgctattt acgccctcca tacggagcta
      721 caaatgcaac tgttcagcaa tcctcaggat taacacaaat gctttggacg gttgatacaa
      781 gagattggga aaatcatagc actgatggca ttatgactaa tgtcaaaaac caattgcaac
      841 cgggaggagt tgttttaatg catgatattc atcaaacaac tatcaatgct ttgccgacag
      901 ttatggaata tctaaaagca gaaggatacg aatgtgtgac tgtatcagaa ctctatgcgc
      961 atcagtaagt aatatcgtta tgtttcttta gaattgatat actaaacaat ccaattactt
     1021 caaaagtaat tggattgttg tttttagtag cccttgatta agttttgttt tttaacaact
     1081 agttgatgaa atgaagttgc tgatttttga gggagatttc agagacttta gcaaattttt
     1141 tcaatttttt gaggtcttca gggtggctaa caaaggtaat aacgatacca gattttccca
     1201 tacggccggt tcgtccagct ctgtgagtgt aattttcctt atcacgagca acatcaaaat
     1261 gaatgacata gtcaagatta tcaatatcaa tgccccgagc aacaagatcc gtagctaaca
     1321 ataaggagag ttggtgggat ttgaattttt cgagaatggt tttacggaat ttgacattaa
     1381 tgtcactcgc taaggaaacc gcagcagctc cattatactg gagacgttct tccgtagcac
     1441 ccaaatcaga taaactgttg aaaaagacca aagctctaaa atgaggaata ttagcaaatt
     1501 ttcgtaataa atcggtacgt tcgcgcttgt caaccatgag gtaaaaatgc tgaatagcat
     1561 cgttggtttg ctctgaaaga tcgatgcaga atgtatttgg agctaaggaa gtttgatcca
     1621 ccttattagt agcactcatg tataccattt gatgatctcg aggaacatag tggctaattt
     1681 tttgaacaaa atcatattgg gaatcaccta ataattcgtc atattcatct aaaacaatgg
     1741 tgttgacaga catcatcttg atttttttta atttaatcag ttcaaaaatg cgtccagggg
     1801 ttccaatcag gatttcagga ccttttttga ggcgttcgat ttgacgtttt tgactggttc
     1861 cagaaataaa gagttgtgcc gttaagccta gtggctgtgc ccaatccttt gtgacttcaa
     1921 aaatttggcc agctaattcg gtattagggg ctaaaatgag tagttgttgg gactttttag
     1981 gcgtcagtgc caatagacta ggaaagacat aagctaaggt cttgcccgtc ccagtagggc
     2041 taataccaag gaaattttta ccatcaacaa taggttgaaa ggcctgttct tggattggcg
     2101 ttaaatgggt aaatgcaact tggtcaagtt tttcttgcca ttgaggggga aatttagtga
     2161 tcatgcgtct tccttttact attgtttcta tattcattat agcattatag tctcaatttt
     2221 ggtaaaatag tgagaaagac tttttagatt tggagtttaa aatgcttaaa aaaccgatta
     2281 ttattggtgt aactggtggt tcaggtggcg gtaaaacaag tgtgtcacgc gctattttgg
     2341 acagttttcc aaatgctcgt attgcgatga ttcaacacga ttcttactat aaagatcagt
     2401 ctcacatgag ttttgaagaa agggtcaaaa ccaattatga ccatcctcta gcttttgata
     2461 cagattttat gattcagcaa ctaaaagaat tattagctgg tagacctgta gatattccta
     2521 tctatgatta caaaaagcat acccgcagta atacgacctt tcgtcaggat cctcaggatg
     2581 ttattattgt tgaaggtatt cttgtcctag aagatgagcg tttgcgagat ttgatggata
     2641 tcaagctctt tgtagacaca gatgacgata ttcgtattat tcgtcgtatt aagcgtgaca
     2701 tgatggaacg tggcagaagc ttagaaagta ttattgatca gtacacaagc gtggtaaagc
     2761 ctatgtatca tcagtttatt gagccaagta aacgctatgc tgatattgtt attccagagg
     2821 gagttagtaa tgtcgttgct attgatgtta ttaatagtaa aattgcgagt attcttggag
     2881 aagtatagtg gaagtggctg agtagattat ttaaatctgc tcttttattg ttgtgctctt
     2941 cctagagaaa gggatcataa aatagaaatg ttattaattt ggcttggatt actatttgtt
     3001 tggaatgaac tcgtgtttgt catatatgcc tttgataaaa gaaaagccat aaaaaagaaa
     3061 cggcgtatta gtgaaagaaa cttattagtg ataacagttt tgtttggagg ttttggagca
     3121 ttacttgcag ctaaaaagta ccaccataaa accagaaagt ggtactttgt catcacttgg
     3181 tatactagta ttttgttgac attactagta acttatatct ttatgaagtg agtcacttaa
     3241 aagaccctta atcagtatta tttagtcaag gctcttttat agtttaaaaa agcacatcat
     3301 cgtcaagggt gttgattttt tgaaaaagat caccaaagat taaaaggatt aatgttccta
     3361 gtaaaactgt catgccagga atgcttgtta gaggagagtt ccagatgaaa tgtagaacca
     3421 gaggacttaa taaccagaaa atttgttgct gttttgaaaa aaggttagaa ccctttaata
     3481 ataagtatgc tccaatggta aaaatagctg tatgtaccca atgtgaagtc acagcagtgc
     3541 taatacgttc aatagcagtc ggtacgagac tatctataga ttttgcttga atcaaataac
     3601 ggatatcttc tgttagttga aagcccatgc cagaaagtaa agcaacaact aatttttctt
     3661 taagagtaag tctaggaaat aaagtaatta ctaaaatagc cgaagaagcc ttaatgggct
     3721 cttcgacaat aggggcagtt aaagcatctc cccaaagctt taaagcgtct ttagaaggga
     3781 tgatgtatga ccaaaattgt cctacgagat ggttcccaca agaagctaaa aaacctgaaa
     3841 tataaagtcc gctaagacag ctaacaataa taacgttcag gttcatatga aattttttac
     3901 ttaaataaaa aatagctgct gcaaatggaa ttaagtaaat gcctaataga ctaatggtta
     3961 gtattagagc aaaagcttct ttaaaggaaa ggttgttagc tgtcagtcct aacaattcaa
     4021 gttcaatacc atttaaaact aaagcaataa aaagaccgta tcgtaaccat ttctattgag
     4081 tgatttgttt cacgaaaacc tcctaatatt aaggtgatat taataatatt gaaaattata
     4141 tcataaacaa cctcaaaaac cattttaaaa actgactgaa gcgaaagata gtcgtaaaaa
     4201 atactcgaat agacccagat atgctataat aaaaggtatg aataagtcta aaaaaattga
     4261 gcaataccaa ctaatgatag agcaggcaaa agagctgttt gcgaatgaat caaatgcttt
     4321 ggctaatttg tcaaatgcca gcgccctgtt aaacatgaca ctacccaatt ctgtttttgc
     4381 gggattttac ctttttgatg ggcaggaatt aattcttggc ccttttcaag gtagggtttc
     4441 ttgcgtccat atcaagcttg gtaaaggtgt ttgtggcgaa tcagctcagt ctcgtcgaac
     4501 cattattata aatgatgtga agcagcatgc caactatatt tcctgtgatg cggcagccat
     4561 gactgaaatt gttgtgccta tggtgaaaga aggccatttg attggggtgc tggacttgga
     4621 ttctagcctt gtagctgatt atgatgaggt tgatcaagaa tacttagagg cctttgtgga
     4681 cctatttctt gaaaagacca cgtttacctt taacatgttt ggagttaaaa actgatgtat
     4741 caagctcttt atcggaaata ccggagccaa acgtttgacg aaatggtggg acaatcggtt
     4801 atttccacaa ctttaaagca ggcagttgaa tctggcaaga ttagccatgc ttatttattt
     4861 tcaggtccta gagggactgg gaaaacaagt gcggcaaaga tttttgcaaa ggccatgaat
     4921 tgtcctaacc aagtcgatgg tgaaccctgt aatcaatgcg atatttgccg agatatcacg
     4981 aatggaagct tagaagatgt gattgaaatt gatgctgcct caaataatgg tgttgatgaa
     5041 attcgtgaca ttcgagacaa atcaacctat gcgccaagtc gtgcgactta caaggtttat
     5101 attattgatg aggttcacat gttatcaaca ggggctttta atgcgctttt gaaaactttg
     5161 gaagaaccga cagaaaatgt tgtctttatc ttggcaacaa cggaattgca taaaattcca
     5221 gccactattt tatctcgtgt gcaacgcttt gaattcaaag ctattaagca aaaagctatt
     5281 cgagagcatt tagcctgggt tttggacaaa gaaggtcttg cctatgaggt ggatgcttta
     5341 aatctcattg caaggcgagc agaaggaggc atgcgtgatg ctttatctat tttagatcag
     5401 gctttgagct tgtcaccaga taatcaggtc accattgcaa ttgccgaaga aattacaggt
     5461 tctatttcca tacttgctct gggtgactat gttcgatatg tctcccaaga acaggctacg
     5521 caagctctgg cagccttaga gaccatttat gatagtggga agagcatgag ccgctttgcg
     5581 acagatttat tgacctatct gcgtgattta ttggtggtta aagctggcgg cgacaatcaa
     5641 cgtcagtcag ctgtttttga taccaatttg tctctctcga tagatcgtat attccaaatg
     5701 ataacagttg ttactagtca tctccctgaa atcaaaaagg gaacccatcc tcggatttat
     5761 gccgaaatga tgactattca gctagctaaa gaagaaggag tagcgttacc atcaggtttg
     5821 tcatcagatg tgacgactga agttgaggga cttaaacgtg aacttaatca gcttaagcaa
     5881 gccttggcac aattgcaagc cagtcccagt ccctcacatg ctcctgtaaa accagcacag
     5941 gctaaaccga aactaaccac ttacaagatt gatcgggtga aaatactgaa aatcatggaa
     6001 gaaacggttc aagatagtca gcaatcacgc caatacttgg atgccctaaa aacagcttgg
     6061 aatgaaattt tagacagtat ctcagctcaa gatagagcct tactaatggg atcagaacct
     6121 gttctggcca atagtgagaa tgctatttta gcttttgaag ctgcttttaa tgctgagcaa
     6181 gcaatgaagc gtactgattt gaatgcgata ttcggcaaca ttatgagcaa agcagcaggc
     6241 ttctcaccta atattttggc agtccctaaa gctgattttc atcacattcg ttctgagttt
     6301 gccttacaat tgaaaactca aaaggcttct gaccctacag aagagacgat agaagaaacc
     6361 cttgagattc cagaaggctt tgattttcta gttgataaaa ttaatgccgt tgaggattaa
     6421 acattattag ctgttaatga accagtaacg ctataaaggt aagcctattg ggcttacctt
     6481 tagcgttttt ttatctaata taccaagata gtttttctag ccaatcctct aatagtagaa
     6541 gtgaaaccaa aaaagattga tgctttcttg ggtatccaag tcagcatcaa tct
//
LOCUS       DAUDYX010000073         6238 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.074, whole genome shotgun sequence.
ACCESSION   DAUDYX010000073 DAUDYX010000000
VERSION     DAUDYX010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.074"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(576..1559)
                     /locus_tag="VHD02_001735"
     CDS             complement(576..1559)
                     /locus_tag="VHD02_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEQ5498324.1"
                     /translation="MVIIAVVIFFGVILGILIVEYGQKRSFSSWLVEKMFAYRKYKLS
                     KKVDNYKGVETQRNRNIPYHLDNPEEIVGFSTQEYDVNGMQVFIINDQKQSHQPVIYY
                     LYGSVYLRRPEKLHFKTVKHLIQLTNAKVVFPNFPKVPYATYQDVYPKLVEAYQQFCL
                     SNGPSQVSVIGDSSGGGMALAFAKYIRDTKTAPQPKQLILISPWLDVSFKNQAYKDFE
                     AEEAYLDARRLRDIGELWAGGQGNMQLPYVSPLYGDYEDLPEITTFVGTKEIFYPDIM
                     SLHDQLTRQRIANQLIIAEKMIHAYVIFPIREAKQARKQIAELVLKTVEHT"
     gene            complement(1590..2840)
                     /gene="dltD"
                     /locus_tag="VHD02_001736"
     CDS             complement(1590..2840)
                     /gene="dltD"
                     /locus_tag="VHD02_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEQ5498325.1"
                     /translation="MLKRLWLILGPLLIAFVLVVITIFSFPTQLDHSIAQEKANAVAI
                     TDSSFKNGLIKRQALSDETCRFVPFFGSSEWSRMDSMHPSVLAERYKRSYRPFLIGKR
                     GSASLSHYYGMQQITNEMQKKKAIFVVSPQWFTAQGINPSAVQMYLSNTQVIEFLLKA
                     RTDKESQFAAKRLLELNPGVSKSNLLKKVSKGKSLSRLDRAILKCQHQVALREESLFS
                     FLGKSTNYEKRILPRVKGLPKVFSYKQLNALATKRGQLATTNNRFGIKNTFYRKRIAP
                     KYNLYKNFQVNYSYLASPEYNDFQLLLSEFAKRKTDVLFVITPVNKAWADYTGLNQDK
                     YQAAVRKIKFQLKSQGFHRIADFSKDGGESYFMQDTIHLGWNGWLAFDKKVQPFLETK
                     QPVPNYKMNPYFYSKIWANRKDLQ"
     gene            complement(2833..3072)
                     /gene="dltC"
                     /locus_tag="VHD02_001737"
     CDS             complement(2833..3072)
                     /gene="dltC"
                     /locus_tag="VHD02_001737"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEQ5498326.1"
                     /translation="MSIEETVIELFDRLFMEDVSEMMDEDLFDAGVLDSLGTVELIVE
                     LESTFNIKVPISEFGRDDWNTVTKIVQGVEELQHA"
     gene            complement(3090..4346)
                     /gene="dltB"
                     /locus_tag="VHD02_001738"
     CDS             complement(3090..4346)
                     /gene="dltB"
                     /locus_tag="VHD02_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEQ5498327.1"
                     /translation="MMMFFSHIPYMEPYGNPIYFVYLILAFLPVIIGIFKQKRLSTYE
                     TLVSLVFILFMFGGDHYQQLVAFLFYLLWQIISVFAYQKYREKANSAGVFYLAIAMAL
                     FPLIWVKVAPLTGPSSQTLFSFLGISYLTFKSIGMIIEMRDGTLQEVRLPDFIRFMIF
                     FPTFSSGPIDRFRRFQEDYHKLPERDDYFAMLNKAVMYLMLGFLYKHIISYCLGGILL
                     PLLENKALMVGGYFNKETILVMYVYGLNLFFDFAGYSMFAIGISYLLGIRTPENFNLP
                     FLSASLKDFWNRWHMSLSFWFRDYVFMRLVHLLIKHKTFKNRNVTSGVAYLVNMLVMG
                     FWHGLTWYYIAYGLFHGIGLIINDAWIRKKKEINRHRKKKGLSPLFQSRAFHVLCIVV
                     TFHVVMFSLLLFSGFLNDLWFNRPLH"
     gene            complement(4343..5881)
                     /gene="dltA"
                     /locus_tag="VHD02_001739"
     CDS             complement(4343..5881)
                     /gene="dltA"
                     /locus_tag="VHD02_001739"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEQ5498328.1"
                     /translation="MIKDMIDSIEQFAQTQADFPVYDCLGERRTYGQLKRDSDSIAAF
                     IDSLALLAKSPVLVFGAQTYDMLATFVALTKSGHAYIPVDVHSAPERILAIIEIAKPS
                     LIIAIEEFPLTIEGISLVSLSEIESAKLAEMPYERTHSVKGDDNYYIIFTSGTTGQPK
                     GVQISHDNLLSFTNWMIEDAAFDVPKQPQMLAQPPYSFDLSVMYWAPTLALGGTLFAL
                     PKELVADFKQLFTTIAQLPVGIWTSTPSFADMAMLSDDFCQAKMPALTHFYFDGEELT
                     VSTARKLFERFPSAKIINAYGPTEATVALSAIEITREMVDNYTRLPIGYPKPDSPTYI
                     IDEDGKELSSGEQGEIIVTGPAVSKGYLNNPEKTAEAFFTFKGQPAYHTGDIGSLTED
                     NILLYGGRLDFQIKYAGYRIELEDVSQQLNQSPMVASAVAVPRYNKEHKVQNLLAYIV
                     VKDGVKERFDRELELTKAIKASVKDHMMSYMMPSKFLYRDSLPLTPNGKIDIKTLINE
                     VNNR"
     gene            complement(5893..6036)
                     /locus_tag="VHD02_001740"
     CDS             complement(5893..6036)
                     /locus_tag="VHD02_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEQ5498329.1"
                     /translation="MIKNKSRMRGIAVFIGKTILFYLILMLLVYFFGYLGHGQSNFIY
                     NEF"
BASE COUNT         2102 a         1229 c         1038 g         1869 t
ORIGIN      
        1 ctgaagaact tgacaatgat attactgcca tcaatgagcg cctaagacga cttcttcaat
       61 agcaatcctc aaaattcctg tttgagctat cccgaacagc ttaacctctg gaactctagt
      121 tatctatgaa ctcgttgcac ttgacttgac aacttgcgag agcatatcag taatttttct
      181 gctaattgaa aacagggtat ttactgtcac acaagtctgt cctttctttt tttctacaac
      241 tttatttcgt cagcgtaaat aaaacaaaag ctctataatc tcttgaatgg gtaaatccac
      301 tgtagagatt atagagcttt ttgaatatca aaaaagtccc ataagattta taatgaaaag
      361 caaccaaact ctcattagaa agactcttat ggaacatatc aatcatacct tacaacacat
      421 cggaattaaa gacaaaaata tcactctaaa caatgttttg aaggatgaat ctcctactga
      481 gctcgttact acttatcaga aaggtagata ggaaatcctg atgaagagtg tgtccaaggg
      541 attatcaatt aggtcttaga ttacaaaata gtaattcagg tatgctctac tgttttcaac
      601 actagttctg caatttgttt tcgagcttgc ttagcttctc ttattggaaa aataacatac
      661 gcatgaatca ttttttctgc aataatcagc tgattagcaa ttcgttgtct tgttaattga
      721 tcatgtaaag acataatgtc tgggtaaaaa atctcttttg tcccaacaaa ggtcgttatt
      781 tcaggcaaat cctcataatc accatacaaa ggactgacat aaggaagttg catattacct
      841 tgaccacctg cccatagttc acctatatca cgaagacgac gagcatctag gtaagcttct
      901 tctgcttcaa aatccttgta ggcttgattt ttaaaactaa catctaacca aggagatata
      961 agaattaatt gctttggctg tggtgctgtt ttagtatctc gtatatactt agcaaacgcc
     1021 aaagccatac ccccaccaga agaatcacca atgacactaa cttgactcgg tccattagac
     1081 aagcaaaatt gctggtaagc ttcaactaat ttaggataga catcttgata agtagcatac
     1141 ggtactttag gaaagttagg aaaaacaacc ttggcattag tcagctgtat aagatgctta
     1201 accgtcttaa aatgaagttt ttcgggtcgt cgtaaataga cactgccata aagataataa
     1261 ataacaggct gatgactttg cttttgatca ttaataataa aaacttgcat cccgttcaca
     1321 tcgtattctt gagttgaaaa acctactatc tcttcaggat tatctagatg ataagggata
     1381 ttacgattac gctgcgtctc aaccccctta taattatcaa cttttttaga taacttatac
     1441 tttctataag caaacatttt ctcaaccaac caacttgaaa aggagcgctt ttgaccatat
     1501 tcaactatca aaatacctag tatcactcca aaaaaaatca ccacagcaat aatgaccact
     1561 tattatcctc gttttctaat attattttac tattgcaagt ctttcctatt tgcccaaatt
     1621 ttactataaa aataagggtt cattttatag ttgggtactg gctgcttcgt ttctagaaat
     1681 ggttgcactt tcttatcaaa agctaaccag ccattccaac cgagatggat ggtatcttgc
     1741 ataaagtagg actcaccacc atcttttgag aagtcagcaa tgcgatgaaa tccttgtgac
     1801 tttaactgga attttatttt acgaactgcc gcttgatact tatcttgatt taagccggta
     1861 taatccgccc aagctttatt aacaggagtt ataacaaaga gtacatctgt ttttcgttta
     1921 gcaaattctg ataacaaaag ctgaaaatca ttgtattctg gtgacgccag gtaactataa
     1981 ttaacttgga aattcttata aagattgtat ttaggtgcta ttcgtttacg ataaaatgta
     2041 tttttaatcc caaaacggtt gttggttgtt gctaattggc ctctcttagt tgctaatgca
     2101 ttcaattgtt tatacgaaaa tactttaggt aatcccttaa cgcgaggcaa aattcttttt
     2161 tcatagttag tagatttgcc taaaaaacta aaaagggact cttctctcaa tgctacttga
     2221 tgttgacatt tcaaaatagc tctgtctaac cgactaagag acttaccctt acttactttt
     2281 ttcaataagt ttgatttaga cacaccaggg ttaagctcaa gcaaacgctt tgctgcaaac
     2341 tgtgattctt tatcagttct agcttttagt aaaaattcaa tcacttgagt gttagacaag
     2401 tacatctgaa ccgcactagg attaatccct tgagcagtaa accattgagg agatactaca
     2461 aagatggctt ttttcttttg catttcattg gtaatttgtt gcataccata ataatgcgac
     2521 aaagatgctg atcctctctt accaattaaa aatggtctat agctccgctt gtagcgctct
     2581 gcaagcaccg aagggtgcat actatccatt cgactccatt cgctagaacc aaaaaaaggc
     2641 acaaaacgac aagtctcatc tgataaagct tgtcttttaa tcaaaccatt tttaaaagaa
     2701 ctatctgtga tcgcaacggc atttgctttt tcctgagcta tggaatgatc aagttgtgtg
     2761 ggaaaactaa aaatagtaat cactactaaa acaaaggcaa taagaagagg acctagaatt
     2821 aaccagagtc tcttaagcat gttgcaattc ctctacacct tgaacaatct tagttactgt
     2881 gttccaatca tcacgaccaa attctgaaat aggtacttta atattgaacg ttgactctaa
     2941 ctcaacaatc aattcgactg tcccaagact atccaacaca cctgcatcaa acaagtcttc
     3001 gtccatcatc tctgaaacat cttccataaa caaacggtca aataattcaa ttacagtttc
     3061 ttcaatagac atttttatac ttccttttat taatgtagtg gtctgttaaa ccaaaggtca
     3121 tttaaaaaac cagagaataa cagaagtgaa aacatgacaa cgtggaaagt taccacgata
     3181 cataagacat ggaatgccct gctttgaaag aggggtgata agcctttttt cttacggtga
     3241 cggttgattt cttttttctt acgaatccaa gcatcattaa taataagtcc tataccatgg
     3301 aaaagcccat aagcgatata ataccaggtt aagccatgcc aaaatcccat gactagcata
     3361 ttaaccagat aggcaacacc tgaagtgaca tttcggtttt taaaggtttt atgcttaatc
     3421 aatagatgaa ccaatcgcat aaacacataa tctctaaacc agaaggacaa actcatgtgc
     3481 catctattcc aaaaatcttt caaacttgca gataagaaag gcaggttaaa gttttctggt
     3541 gttcgaatac ccaataggta ggaaatacca atcgcaaaca tcgaataacc agcaaagtca
     3601 aaaaagagat tcaagccata cacgtacata accagaatag tttccttatt aaagtagcca
     3661 ccaaccatca aagccttatt ttcaaggaga ggaagtaaga tgccacccaa acaataggaa
     3721 atgatatgct tgtataggaa acctaacatg agatacatga ctgccttatt gagcattgca
     3781 aaataatcat cacgttcagg aagtttatga taatcttcct gaaaacgtct gaagcgatca
     3841 atggggccac ttgagaaagt ggggaagaaa atcatgaagc gaataaaatc cggtaaacga
     3901 acctcttgga gagtcccgtc acgcatctca ataatcattc caatactttt aaaggtcaaa
     3961 tatgaaattc ctagaaaact aaagagtgtt tgagaagaag gacctgttag gggagcaact
     4021 ttgacccaga taagggggaa taaggccatc gcaatagcta agtaaaacac tcctgcacta
     4081 ttagctttct cacggtattt ctggtaagca aaaacactga tgatttgcca caaaagataa
     4141 aagagaaaag caactaattg ttgataatga tctcctccaa acataaagag aataaatacc
     4201 aaactcacca aggtttcata agtgcttaag cgcttttgtt taaaaatccc aatgatgact
     4261 ggtaagaaag ccaaaataag atagacaaag taaatggggt taccgtaagg ttccatgtag
     4321 ggaatgtggc taaaaaacat catcatcggt tattgacctc attaatcaaa gttttaatat
     4381 ctatcttacc atttggagtt aatggtagac tatctcgata aagaaactta gaaggcatca
     4441 tataactcat catatgatcc ttaacagagg ctttgatagc tttggttaac tccaattccc
     4501 tatcaaaacg ctctttaacc ccatctttga caacgatata ggccaagaga ttttgaacct
     4561 tatgttcttt attgtacctt ggaacagcca cagccgatgc taccattggt gattgattca
     4621 actgttggga aacatcttct agttcaatac gataaccagc atatttaatc tgaaaatcca
     4681 aacgcccacc atacagcaaa atattatctt ctgtcaatga gccaatgtcc cctgtatgat
     4741 aagcaggctg tcctttaaaa gtaaagaaag cttctgcggt tttctcagga ttgttgaggt
     4801 aaccttttga cacagcaggc cctgtaacaa taatttcacc ctgctcccct gaagataact
     4861 ccttaccatc ttcatcaata atataggtcg gagaatctgg tttggggtag ccaatcggca
     4921 agcgggtata attatccacc atttctctag taatttcaat agcggataag gctacagtcg
     4981 cttctgttgg tccataggca ttgataatct tggcactcgg gaaacgttca aagagttttc
     5041 gagccgtaga aacggttaat tcttcgccat caaaataaaa atgcgttaaa gctggcatct
     5101 tagcctggca aaaatcatcg ctaagcatgg ccatatcagc aaatgaagga gtcgatgtcc
     5161 aaatgccaac tggaagttgc gcaatcgttg taaataactg tttaaagtct gccaccaatt
     5221 cttttggaag tgcaaataag gttccaccta aagccaaggt aggtgcccag tacatgacag
     5281 aaaggtcaaa ggaatagggt ggctgcgcca gcatttgtgg ctgtttaggt acatcaaatg
     5341 ctgcatcttc gatcatccag ttggtaaagc tcaaaagatt gtcatgtgaa atttgcaccc
     5401 cttttggttg gcccgttgtt ccagaggtga aaataatgta ataattatca tctcctttaa
     5461 cagaatgtgt tcgctcatag ggcatttctg ctaattttgc tgactcaatc tctgatagtg
     5521 agacaaggga aatcccttca atagtaagag ggaattcctc aatagcaatg attaaactgg
     5581 gctttgcaat ctcaataatc gctaaaatac gttctggtgc tgaatgcaca tctactggga
     5641 tataggcatg accagatttg gttaaagcta caaaagtagc taacatatca taagtttgcg
     5701 ccccaaaaac caaaactgga gattttgcca gtaaagctaa gctatctata aatgcagcaa
     5761 tgctatcaga atctcttttg agttgtccgt aagtgcggcg ttcccctaaa caatcataaa
     5821 ctggaaaatc agcctgtgtc tgagcaaact gctcaatact gtcaatcata tccttaatca
     5881 tcagtcaatc tcctaaaatt cattataaat aaagttactt tgaccatgtc ctagataacc
     5941 aaagaaatag accagcaaca ttaaaatcag ataaaataaa attgtcttgc caataaaaac
     6001 agcaattcct ctcattcttg atttgttttt tatcatactt taccttcttg tattagtgaa
     6061 ataatgtcct aaaaaattct actcgaaaaa acctaacttg acaagctaaa agtcatatta
     6121 ttaaaaagaa aattttttta aagcttaact atttagacag ttaaatttta tgccttttta
     6181 ctaaacttac taagttaatc tttactttaa aaagttaact taaaattgtc ctaataaa
//
LOCUS       DAUDYX010000074         6123 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.075, whole genome shotgun sequence.
ACCESSION   DAUDYX010000074 DAUDYX010000000
VERSION     DAUDYX010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.075"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(114..1445)
                     /locus_tag="VHD02_001741"
     CDS             complement(114..1445)
                     /locus_tag="VHD02_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEQ5498330.1"
                     /translation="MAITKIHPIKSTLNLAIDYITNEEKTDEKILVSTHNCFASTAHT
                     SFFKTREDNKVSGSVLARHLIQSFLPGEATPEMAHQIGLELCKKILKDEYEFVLSTHI
                     DKGHIHNHIIFNNVNMVTGKCYQSNKRSYHQIRYQSDKLCKENNLSVIDGFYESYKRK
                     YKTNGKSWYENEQSKKGTSWKSKLQFDIDRMIKQSKSWEEFLKKMVELGYEIKYGKHI
                     AFKYKDKERFTRAKTIGEDYIEDRLKERIVENATRRIYTVKKRVGNIIDITNNEKAQV
                     SKGYEYWATKHNLKVATDMVVLMRDKGFNSLAQLDNFIKESADKRQELQNKIKVVDGK
                     ISTLSATMEQVHAVTKYRQIYLAYKKDTTDKAFYDEYKSQIILYQNALAELKKSYTKL
                     PNSNDILKELDSLYEKKNTLMQEYSSSKSDMKELYQIRKNYEKYMGKEIER"
     gene            complement(1446..1805)
                     /locus_tag="VHD02_001742"
     CDS             complement(1446..1805)
                     /locus_tag="VHD02_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEQ5498331.1"
                     /translation="MANRIRNVQLKINLTEEEKALFEKKMKMAKCKTMSHFLRKVVSE
                     SDIYVVDLQPFREIQGLLFRYASSVNQIAKRVNSTGVIYSNDIKDMQSQIEHLSKEIW
                     QIHSLLLNKTTNKGDDI"
     gene            2140..2400
                     /locus_tag="VHD02_001743"
     CDS             2140..2400
                     /locus_tag="VHD02_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEQ5498332.1"
                     /translation="MDYYDKFKALSDPIRLDILNELNNGELSAGDIADKFALPNSKIS
                     YHLAILKKVDMVTERKYKNFIFYSLNRNGIQEASTWFDRFQK"
     gene            2694..3107
                     /locus_tag="VHD02_001744"
     CDS             2694..3107
                     /locus_tag="VHD02_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEQ5498333.1"
                     /translation="MNKEYFLFVNGKKIKVSEEIYKVYWQEKNHENYLKQIDRKNHLL
                     LFSSFDHDGHFEDSIVDEGFDVDKIVQTQMMIEAVRDALSKLNDEEREIIDRLYFNDE
                     TIRSVAETKKISHPALIKRRNKILEKLRELLKEFR"
     gene            3225..3605
                     /locus_tag="VHD02_001745"
     CDS             3225..3605
                     /locus_tag="VHD02_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ5498334.1"
                     /translation="MNIQINNSSDDPIYLQIKNQIKAQIISGDLKVGEQLPSIRFLAK
                     ELRVSMLTAKRAFDELELDGFINSVQGKGNFVAAQNKELIREEYLKKIESKLQEVVEL
                     SEIADVSSDELIEMLKSYVEGEYE"
     gene            3598..4446
                     /locus_tag="VHD02_001746"
     CDS             3598..4446
                     /locus_tag="VHD02_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5498335.1"
                     /translation="MSKYAIEIKDLVKTFDSFKLGPVNLTIPKGTIVGYIGQNGAGKS
                     TTIKLLLGLLRKDSGDIRLLDEENPNSVELKDKLGVVFDDLLVPEEMTLIDVEKFCHR
                     VYSKWDKKQFYGLVEKFNLPHKKMIKNYSRGMKMKLSMAVALSHNAELLILDEATSGL
                     DPIVREEILDLLLDFMQDENHTILISSHILSDLEKVADYIAFIHNGKILFMETKDELK
                     ENYGICTLSNEEVNNIDEEAIVGRRIHSFGQELLVKKSLVPDGIKLQKPSIEDIMIYF
                     VKGVRQ"
     gene            4446..5075
                     /locus_tag="VHD02_001747"
     CDS             4446..5075
                     /locus_tag="VHD02_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HEQ5498336.1"
                     /translation="MTALILKDIATLKKTLLLTVTISIALAVYGIYENELFMVPLICV
                     MIPLILTAIAFGYDTKSNFEQFAFSMPIKKSSYVLSKFFFAFAFGLVGSICIFVLLMT
                     KNQMSLDNIVFIALITLAASILMSAIQLPFILKYGAEKGRLIMVITYFLIFALSTLLK
                     EKSNFLAELMEIFSKNSISMICLGILVVSILIIGVAVKLSVMIMEKKEY"
     gene            5122..5487
                     /locus_tag="VHD02_001748"
     CDS             5122..5487
                     /locus_tag="VHD02_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498337.1"
                     /translation="MFYIAAIIFLVLGLADLSKSFMLKSNTDYVKAEVVSIWQPNPEA
                     VKKGNSKWANFMYLVDGKQYISSNRIQVSMNTKVGDLKQIKYDKRNPEKIYGFSVKRA
                     CILFIVAIVLFIIAKFKLF"
     gene            5666..>6123
                     /locus_tag="VHD02_001749"
     CDS             5666..>6123
                     /locus_tag="VHD02_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEQ5498338.1"
                     /translation="MLRNEIQKKTGLTRKAIEYYEEKGLINPQKTENGYRDYSENDLE
                     VLIQISLFRKLGISVTEIEGYLTTGISSLSSVLRRKQHQLDVEEKRKEVLELVVKGEN
                     QELINEKIKLIEAEESIYERLERLFPGYFGQMLFVAYQPFLNESLGKDEE"
BASE COUNT         2110 a          801 c         1107 g         2105 t
ORIGIN      
        1 ataagttctg ttgatgaatt acaaagcaaa atacaggaaa attacgaaaa agttattcct
       61 aaaataagtg ttatatcgag gtttattgtt gaaaaaatga taagatgaga aagctacctc
      121 tctatctcct tacccatata tttctcgtaa ttttttctga tctgatacag ttctttcata
      181 tcagattttg atgaagagta ctcttgcatt agggtattct ttttttcgta cagtgaatca
      241 agctctttta aaatatcatt agaattaggg agctttgtgt aggatttttt gagttctgca
      301 agggcgttct gatacaagat aatctgtgat ttatattcat catagaatgc tttatcagtc
      361 gtatctttct tataagctag ataaatttga cgatacttcg taacagcatg aacctgttcc
      421 atagtggcag atagggtaga aattttccca tcaaccactt tgattttgtt ttgtaattct
      481 tgtcttttat cggcactttc tttaataaaa ttatcaagct gagcaagaga attaaagcct
      541 ttatcacgca ttaaaacaac catatcagtt gcgactttca gattgtgttt cgtagcccag
      601 tattcataac ctttgcttac ctgagccttt tcattattcg taatatcaat gatatttcca
      661 acacgctttt tcactgtata gattcttcga gtagcatttt ctacaatgcg ttcttttagt
      721 ctatcttcta tataatcttc accaatcgtc tttgctcttg taaatctttc tttgtcttta
      781 tacttaaagg cgatgtgttt gccatacttt atttcataac caagttcaac catcttcttt
      841 agaaattctt cccaagactt agactgtttt atcattctat caatgtcaaa ttgtagctta
      901 cttttccaag aagtaccttt cttagattgc tcattttcat accatgattt gccattcgtt
      961 ttgtactttc tcttatagct ttcgtagaac ccatcaatca cagataggtt gttttctttg
     1021 catagcttat cgctttgata cctgatttga tgatagcttc tcttgttgga ctggtagcac
     1081 ttgcctgtaa ccatatttac attattgaat atgatgtgat tgtggatatg ccctttatct
     1141 atgtgagtag atagtacaaa ttcgtattcg tcctttaata tctttttaca cagttcaaga
     1201 ccgatttgat gtgccatttc gggcgttgct tcaccgggta gaaaagattg aataaggtgt
     1261 cttgcaagta cagaaccact cactttgtta tcttctcttg tcttaaaaaa agatgtatga
     1321 gcagtagaag caaagcagtt gtgagtgctt accaaaatct tttcatcggt tttttcttcg
     1381 ttggtaatat aatcaatagc aagattaagt gtagatttta taggatgtat ttttgtgatt
     1441 gccacttata tgtcatctcc tttgttggta gttttattga gcagtaggga atgtatttgc
     1501 catatttctt ttgatagatg ctcaatttgg gattgcatat ctttgatgtc gttgctatag
     1561 ataacaccag tagaattaac tcgtttagca atttgattta cactacttgc atacctaaaa
     1621 agtaatcctt gtatttctct aaatggctgt aaatcgacaa cataaatatc tgattccgac
     1681 actacttttc ttagaaaatg gctcatggtc ttacactttg ccatcttcat tttcttttca
     1741 aaaagtgctt tttcttcttc tgttaagttt attttcaact gaacatttct aattctgttt
     1801 gccatgtttg ttacttcctt tcaaaaaaat tggggtctta gggtactccc taacaagcta
     1861 aaattgataa aaaaagaagc aactccacga ggaggctgct tcatcgattt ttcgtaagtg
     1921 ggtacacact tactgtgctt gctacataag aatgattagt aaaacaagcg ttaagcgaga
     1981 tattagtttg tgtcttaatt ataccaagta tagaagttaa tgtcagcgtg ttcagggaac
     2041 tatttctaaa aaaattgaaa tagttattga gttaagtcct atgatgatgt tataattatt
     2101 ttgaaaaaaa ttgaaataga ttatggaaag gctaaactaa tggattatta tgataagttt
     2161 aaagctctat ccgatccgat aagactggat atattaaatg aattgaataa tggagagtta
     2221 tctgcagggg atattgcaga taaatttgct ctaccaaatt caaaaatttc gtatcattta
     2281 gcgatactaa aaaaagtaga tatggttaca gaaagaaaat ataagaattt tattttttat
     2341 agcctaaatc gaaatggtat tcaggaagct tcaacttggt ttgatagatt tcaaaaataa
     2401 aattcgcata aataagcttt ctaaaattag caattacaag gagatgaaga tttaactgtt
     2461 aattaggagg atgatgtgca tgggatgggt tacgcacgat ttaataaaag ggattcataa
     2521 ttttttgtgc ggaaaatttt ccttattgca tttatcctct acctaatccc aaatttaata
     2581 aattaaatag tagaactgtt tttgttcatt tgagaggact cggtgtgcca tcatgccgta
     2641 gtcctccttt tttgtcttca aacaagattt tgaaacgaaa aggaggacga aaaatgaata
     2701 aagaatattt cttgtttgta aacggaaaga agatcaaagt tagcgaggag atttataaag
     2761 tctattggca agagaaaaat catgagaatt acttgaagca gatagatcga aaaaatcatc
     2821 tgcttttatt ttcttctttt gaccatgatg ggcattttga agatagcatc gttgatgagg
     2881 gatttgatgt agataagatt gttcagacac aaatgatgat agaggcagta agagatgcac
     2941 tatcaaaatt aaatgatgaa gaaagagaaa tcattgatag actttatttc aatgatgaaa
     3001 ccattcgttc agtagctgaa actaaaaaaa tatctcatcc agctctcata aagagaagaa
     3061 ataagatatt ggaaaagcta agagagttgc tgaaagaatt tagatagtaa tgagaagaag
     3121 cgggcaaaga aaattttgtc tgcttctttt tagatattga aatgttttaa ctgttatgct
     3181 ataataataa cagttaaaac acaaataaca gtaggaggtt gaggatgaat atacagataa
     3241 ataattccag tgatgatcca atatatctgc aaataaaaaa tcaaattaaa gcacaaatca
     3301 tttctggaga tttgaaagtg ggagaacaac taccttcgat aagattctta gcgaaagaat
     3361 taagagtaag tatgttaact gcaaaaagag cttttgatga attagagcta gacggattta
     3421 taaattctgt acaaggaaaa ggcaattttg ttgcagcaca aaataaagaa ctaattcgag
     3481 aagaatactt aaagaaaatt gaatcgaagc ttcaagaagt agttgaactt tcagaaattg
     3541 cagatgtatc cagtgatgaa ttgatagaaa tgttaaagag ttatgtggag ggtgaatatg
     3601 agtaaatatg ctattgaaat taaagattta gtaaaaacat ttgacagttt taaactagga
     3661 ccggtaaatc ttactattcc gaaaggaaca atagttggat atatcgggca gaatggagct
     3721 ggtaaaagta caactataaa attgttactt gggcttttaa gaaaagactc tggcgatata
     3781 agacttttag atgaggaaaa tcctaatagt gttgaactga aagataaact tggagtggta
     3841 tttgatgatt tgttagtgcc ggaagaaatg acattaatcg atgttgaaaa gttttgtcat
     3901 agagtttatt caaaatggga taaaaagcag ttttatggac tcgttgaaaa attcaattta
     3961 ccacataaaa agatgattaa aaattattct cgtggtatga aaatgaaact ttcaatggct
     4021 gttgctttat ctcacaatgc tgaacttttg attttagatg aagcaacaag tgggcttgat
     4081 ccaattgtaa gagaggaaat cttggatttg ttattagatt ttatgcagga tgaaaatcat
     4141 acaattctta tttcttccca tattttatct gacttagaaa aagtggcaga ttacattgct
     4201 tttattcaca atggaaaaat cttatttatg gaaacaaaag atgagttaaa agaaaactac
     4261 ggtatatgta ctttatccaa tgaagaagta aataacattg atgaagaagc cattgtagga
     4321 agaagaatac attcttttgg gcaagagtta cttgttaaaa agagtcttgt acctgatggt
     4381 atcaagttac aaaaaccgtc aattgaggat attatgattt actttgtgaa aggagtgaga
     4441 caataatgac agcattaatt ttgaaagata tagctacttt aaaaaagaca ttattattaa
     4501 cggttactat cagtattgca ctggctgtat atggaattta tgaaaatgaa ttgtttatgg
     4561 ttccgttaat atgtgtaatg attccgctca tacttactgc aatcgccttt ggctatgata
     4621 caaaatcaaa tttcgaacag tttgcatttt cgatgccaat aaaaaagagc agttatgtgt
     4681 tgagcaagtt tttctttgca tttgcctttg gtttggttgg ttcaatctgc atttttgtac
     4741 tgttgatgac aaaaaatcaa atgtcattgg ataatatagt gtttattgct ttaattacat
     4801 tagctgctag tatacttatg tctgccattc aattaccatt tattcttaaa tatggtgctg
     4861 aaaagggtag actcattatg gtgatcactt atttcttaat atttgcttta tcaacactgt
     4921 taaaagaaaa gtcaaatttt cttgcagaac taatggagat ttttagtaag aattctatat
     4981 caatgatttg cttaggaatt ttagttgtaa gcatattaat cataggggtt gcagtaaaat
     5041 tgtccgttat gattatggag aaaaaagaat actagttata aattatttaa tttgtagtta
     5101 ggtggtagag atggaaaaac aattttttat atagctgcta ttatattttt agtattaggt
     5161 ttagcggact taagtaaaag ttttatgctt aaaagtaata cagattatgt aaaagcagag
     5221 gttgtatcta tatggcaacc aaatccagag gctgtgaaaa aaggaaattc caaatgggca
     5281 aattttatgt acttagttga tggtaaacaa tatatatcta gtaacagaat acaggtttct
     5341 atgaatacga aagtaggaga cttaaagcaa ataaaatatg ataaaagaaa tcctgaaaaa
     5401 atttatgggt tttcagtaaa aagagcatgt attcttttta tcgttgcaat tgttttattt
     5461 ataattgcca agtttaagtt gttttagtgg tggtgattaa tatgcctaaa attccatcaa
     5521 ttgtagtctt ttgtatgata tgtattccga taataagatt aatttatttg gctataaaaa
     5581 agaaaaaata aaatacgagt aagttttttg cttgacctgt ccattgggga cagtgctatc
     5641 ttttagttaa aggaggtggc aaaccatgtt aagaaatgaa attcaaaaaa aaacaggttt
     5701 aacaagaaaa gcaattgaat attatgagga gaaaggactt ataaatcctc aaaaaactga
     5761 aaacggttat agagattaca gcgagaatga tttagaggtt ttaattcaaa tatctttatt
     5821 tagaaaactt ggaattagtg taactgaaat agaagggtat ctaaccactg gcattagttc
     5881 attatcatct gttttaagaa gaaagcaaca tcaattagat gttgaagaga aaagaaaaga
     5941 agtccttgaa cttgtagtta aaggtgaaaa tcaggaactg ataaatgaaa aaattaaatt
     6001 gattgaagca gaggaatcaa tctacgaaag attagagaga ttatttccgg gatattttgg
     6061 acaaatgtta ttcgtagcat atcaaccatt tttaaatgaa tcattaggaa aagatgagga
     6121 aga
//
LOCUS       DAUDYX010000075         5621 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.076, whole genome shotgun sequence.
ACCESSION   DAUDYX010000075 DAUDYX010000000
VERSION     DAUDYX010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.076"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(<1..132)
                     /locus_tag="VHD02_001750"
     CDS             complement(<1..132)
                     /locus_tag="VHD02_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEQ5498339.1"
                     /translation="MGLLDLIGRKRARDKPQNSYEGQDFFYLFGRTTSGENVDEFKGI
                     "
     gene            complement(237..509)
                     /locus_tag="VHD02_001751"
                     /pseudo
     CDS             complement(237..509)
                     /locus_tag="VHD02_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424370.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(528..833)
                     /locus_tag="VHD02_001752"
     CDS             complement(528..833)
                     /locus_tag="VHD02_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEQ5498340.1"
                     /translation="MKEYQVTMSDDAKADLLSIYHYVRDELCAPQAADNLLERLSQEM
                     LSLSIFPERCAIIEDLIGKGYTFRQLIVKKYRIVYHVLEDEVIIVAVVYGSRHMNNW"
     gene            complement(833..1135)
                     /locus_tag="VHD02_001753"
     CDS             complement(833..1135)
                     /locus_tag="VHD02_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEQ5498341.1"
                     /translation="MAKTGTLNLRVDDSVKSAADDILKRLGIPMSTAIDMFLNQIILT
                     GGIPFDVSLPEAPQRVNVDYMSQEKFYDKLISSFEDAKTGNRKDVREFLSQLKENA"
     gene            complement(1190..1366)
                     /locus_tag="VHD02_001754"
     CDS             complement(1190..1366)
                     /locus_tag="VHD02_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEQ5498342.1"
                     /translation="MGQNVVMRQAPGGNIKPDKEKSVEKIDGIVGLILGLDRCIRRQG
                     DEASVYDERGILSF"
     gene            1382..2275
                     /locus_tag="VHD02_001755"
     CDS             1382..2275
                     /locus_tag="VHD02_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498343.1"
                     /translation="MTTMLDFPFHHEFIEFLGRWTHILITLLKRHHGQADTAIPRYLL
                     VTIREKTGIVKSTTVQNTAFTTQSNQLKKQYGQFLLTESNNCCACPGCDRPLILTSGN
                     LAAENYEVSAIEKDKDAEPLNLIALCPDCFLSYQADSRKKIVTALKNVKKILVSAHNS
                     QQAISDMKLDSGIVSVLTSLNKLKCDEYDISYDSKQLTDKISPENNRTLYQLVKNPVI
                     DNYLTIQKIIVNLDKQGKIDYEEIQYQTRSMYKKLKAAKHNNLAIFNTISEKLHKATL
                     QDIYFCQTIVSYFIQKCEVLE"
     gene            2275..2499
                     /locus_tag="VHD02_001756"
     CDS             2275..2499
                     /locus_tag="VHD02_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498344.1"
                     /translation="MQLPNKLYSYQKSTLAYLPRVLNEIKSGNSNVKDIFHAISEELD
                     DPTDFLSIMDCLYALNAIEMSNEGEVKLCL"
     gene            2490..3662
                     /locus_tag="VHD02_001757"
     CDS             2490..3662
                     /locus_tag="VHD02_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEQ5498345.1"
                     /translation="MLIEMWSPVFKNHGEIRESICFHYGLNVIMGMDLADNSIGKSSS
                     LLAIDFIFGGNSYLKSIAVKKLGDHPIYFCFQFEKKFYFSGDTANPDIILVCDKSYSP
                     TGETMDLGIFLNKLKKRYHLDSPELSFRLAMSGFFRIAGKSNQNTDFPLQVYSSQKSS
                     ESITTLIQLFNLYDNIARYKERLKDKSDQLTTFRNARKYTFISNLVGGKKQFEANVSE
                     IKRLEYDLAHLQDTHQDKIDSDDIEKNQQKLQLRNTKLELESSLRDKQRRLKLLDISI
                     EFGLYPTESDLTELQQYFPDTNLKKLYEVEAYHKKLETILDSEFSTERESLIAEIDEL
                     ESQLTTLNQELQELGNIPNLSSEYLENYSKLTATINALKEQNEAYLKESGLSKEKS"
     gene            3675..4148
                     /locus_tag="VHD02_001758"
     CDS             3675..4148
                     /locus_tag="VHD02_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
                     /protein_id="HEQ5498346.1"
                     /translation="MKRSTEDILLDLEVKINSKMREVNNILYPDIRKAPQINLNAHNS
                     YSFYTPDDDGTGTKFKGMIVFDLVMLYLTNLPALAHDSLLLSNVSYQATEALLKLYDQ
                     SRSLNKQVFLAFDKASSYSPDANQLLSENTVLRLSSNGNELYGISWNKGENSDEI"
     gene            4138..4347
                     /locus_tag="VHD02_001759"
     CDS             4138..4347
                     /locus_tag="VHD02_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ5498347.1"
                     /translation="MRFSYNKLWKLLIDKGWTKTKLRQEAGISSSTIAKLGKGENITT
                     DILLEICIALDCKIEDIVEIVDNNI"
     gene            complement(4450..5589)
                     /locus_tag="VHD02_001760"
     CDS             complement(4450..5589)
                     /locus_tag="VHD02_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ5498348.1"
                     /translation="MFDFANNTFLAGLNPASLSLVAVYQSLESVIGVLFNLFGGVIAD
                     SFKRKKIIITTNILCGTACLVLSFLTKEQWLVYAIVLTNVILAFMSAFSSPSYKAFTK
                     EIVKKDSISQLNSLLETTSTVIKVTVPMVAIFLYKLLGIHGVLLLDGLSFLIAALLIS
                     FILPVNDEVVIKEKVTIREIFNDLKIGFKYVYSHKSIFIITVLSALVNFFLAAYNLLL
                     PYSNQMFGEISTGLYGTFLTAEAIGGFIGAILSGFVNKELSSMRLILFLSLSGLMLML
                     APPFYIMFHNAIILALSPALFSLFLSIFNIQFFSLVQKDVDNDFLGRVFGIIFTITIL
                     FMPIGTGFFSVALNPNNSFNLFIIGSCITILSLVFRILFKKYNIR"
BASE COUNT         1971 a         1094 c          909 g         1647 t
ORIGIN      
        1 aattccttta aactcatcca cattctcgcc actagtcgtg cgaccaaaga ggtagaaaaa
       61 gtcctgacct tcataactgt tttgaggctt atctctagcc ctttttcttc caattaaatc
      121 aagtagtccc ataagccctc cttattttgg ggtacaaaaa aagcacctcc tttgaagtgc
      181 tttcggtata tttctttcaa agtaatcaac agtctttctt acaaaaccta gtattctcat
      241 tctgtcaatt ccttaaatgc gtcaagatgt cgtgataata ctgaagtcgc aatctcatct
      301 aaccttgctt gttcaacaac tgttgtcatc tgctcatcac tgcttaaact ttggtgatcc
      361 gctgatagtt tgctatcaga tggacttaat accaaagttc catcagttga tttataaagg
      421 ttaaactcct aattctcttg aatgccaaat gggtcaggta tcggaagata gatgtcatca
      481 ccaatttgta tcgtctttac gagttccata catcactctc ccttacttta ccaattattc
      541 atatgacgtg atccatagac gaccgcaaca attataactt catcttccag aacatggtat
      601 acaatgcgat attttttgac aatcagttgt ctgaaggtat agccttttcc aatcagatcc
      661 tcaatgatgg cacaacgttc aggaaaaatg gatagtgata acatttcttg ggataacctc
      721 tcaagaagat tatccgctgc ctgtggtgca cagagttcat cacgaacata gtgatagatg
      781 ctcagcaaat ctgctttagc atcatccgac atggtgacct gatattcttt cattaagcat
      841 tttccttcaa ttgagataga aattctctga catccttgcg gttgccagtt ttcgcatctt
      901 caaagctgga gataagctta tcataaaact tctcctgact catgtaatca acattaactc
      961 gttgaggcgc ttcaggtaga gaaacatcaa atgggatacc cccagtcaaa ataatctgat
     1021 tcaaaaacat atcaatcgca gttgacatag gaatacctaa gcgtttcaaa atatcatctg
     1081 ccgcactttt tactgaatca tcaactcgta aattcaaagt tcccgtttta gccatggtta
     1141 tcctcctttt ttgtaacgac attgtatcac gtttttagag ttgactcaac taaaaactca
     1201 atatccctcg ctcatcatag acgctagctt catcaccttg gcggcgaatg caacggtcaa
     1261 gccccaggat aagacctaca ataccgtcaa tcttctcaac agatttttcc ttatctggct
     1321 tgatgttgcc tccaggggct tgtcgcatca ccacgttttg tcccatccat ttaaggactg
     1381 gatgaccacc atgttagatt ttcccttcca tcatgagttt atagagttcc ttggacggtg
     1441 gactcatatc cttataactc tgctcaaaag gcaccatggt caagccgaca ccgcaattcc
     1501 aaggtatctt ctagtaacca taagagagaa gacagggatt gtaaaatcaa ccactgtgca
     1561 aaatactgcg ttcactaccc agtcgaatca gctcaaaaaa caatacgggc agttcttgtt
     1621 aacagaatct aataactgtt gtgcatgccc aggctgtgat agaccactca ttctaaccag
     1681 tggcaactta gcagcagaaa attacgaggt ttcagccatt gaaaaagata aggacgctga
     1741 accactgaat ctgatagccc tctgcccaga ttgtttttta tcctatcaag cagatagtcg
     1801 caaaaaaatt gtaacagctc taaaaaatgt taaaaagatt ctagtctctg cacataacag
     1861 ccaacaagcg atttctgata tgaagttaga cagtggaatt gtttcagttt tgactagtct
     1921 caataaacta aaatgtgatg aatatgatat ctcctatgac tcgaagcaat tgactgataa
     1981 gatatcacct gagaacaatc gtacgcttta tcaattggtt aaaaacccag ttattgataa
     2041 ctacttaacc attcaaaaaa tcatcgtaaa tttggacaaa caaggaaaaa ttgattacga
     2101 ggaaatccaa tatcaaacgc gatcgatgta taaaaagtta aaagcagcca agcataataa
     2161 tctggctatt ttcaatacta tttcggaaaa actccataaa gctacactac aggacatcta
     2221 cttctgtcag acgattgtct cttactttat ccaaaaatgc gaggtacttg aataatgcaa
     2281 ttaccaaata aactttactc ctatcaaaaa agcacccttg cttatttgcc tagagtgcta
     2341 aatgaaatca aaagtggaaa ctcaaacgtc aaagatatct ttcacgctat ctcagaagag
     2401 ttggatgatc ctacagattt cctatctata atggattgct tgtatgcatt gaacgctatt
     2461 gaaatgtcta atgaaggaga agtaaaatta tgcttataga aatgtggtct ccagtattta
     2521 agaatcacgg agaaattcgg gaatcaatct gcttccacta tgggttaaac gtgattatgg
     2581 ggatggattt agctgataac tctatcggca aatcctcctc actattagcc attgatttca
     2641 tctttggagg taatagctat ctaaaatcaa ttgcggttaa aaaattaggt gatcatccta
     2701 tctatttctg ctttcaattt gagaaaaagt tttatttttc aggagatacg gccaacccag
     2761 atatcattct agtgtgtgac aagagctact ctccaaccgg tgaaacaatg gacctaggaa
     2821 tttttcttaa caaacttaag aaacgatatc atttagattc tccagagcta tcattccgat
     2881 tagctatgag tggcttcttt aggattgcag gaaaaagtaa tcaaaatacc gatttccctt
     2941 tacaagttta ttcgagccaa aaatcttctg aatctattac tactctcatc caactgttta
     3001 atttgtatga taacattgca cgatacaaag aacgtctgaa agataagagc gaccagttaa
     3061 caacttttcg taatgctcga aagtatacct tcatctccaa cctggttggc ggtaaaaagc
     3121 aatttgaagc aaatgtctca gaaataaaaa gattggaata tgatttagca catctccaag
     3181 acactcatca agataaaatt gactctgatg atattgagaa aaatcaacaa aaactccagc
     3241 ttagaaatac taagctggag ttagaaagca gtcttcggga caagcaaaga cgtcttaagc
     3301 tattggacat cagtatcgaa tttggcttat atcctactga atctgactta acagagcttc
     3361 aacaatactt cccagatact aatctcaaaa aattatatga agttgaagct taccacaaaa
     3421 aattggaaac tattttagat tccgaattct caactgaacg tgaatcccta atagctgaga
     3481 tcgacgaatt agagagtcaa ttaacaactc taaaccaaga acttcaagaa ctaggaaaca
     3541 tcccaaatct ttcaagtgag tacttagaaa actactctaa actaacagct acaatcaatg
     3601 cactaaaaga acaaaacgag gcctacttaa aggagtctgg tctatccaaa gaaaaatcat
     3661 aagcggacgc tgacttgaaa cgcagtacag aagatatcct tctagatcta gaagtaaaaa
     3721 tcaacagtaa gatgcgtgaa gtcaataaca ttctatatcc agatattcgc aaagctcctc
     3781 aaattaacct aaatgctcac aatagctact ccttctatac accagatgat gatggtacag
     3841 gaactaagtt taaagggatg attgtattcg acttagtaat gctctacctt actaatctgc
     3901 cagcactcgc acacgattca ttactcttga gtaatgttag ctaccaagcg actgaagcac
     3961 ttttgaagct gtatgaccag tcaaggtcat tgaacaaaca agtcttttta gccttcgata
     4021 aagccagctc ttattctcca gatgctaatc aactcttgtc agaaaatacg gtattgagac
     4081 tctctagtaa cggaaatgaa ctctacggta tttcatggaa caaaggagaa aactcagatg
     4141 agatttagtt acaataaatt atggaaactt cttattgata aaggttggac caaaacaaag
     4201 ctcaggcaag aagctgggat tagttcttcc acgatcgcaa aattgggaaa aggggaaaat
     4261 atcactactg atattttatt ggaaatttgt attgcacttg attgtaagat tgaagatata
     4321 gtagaaattg tagataataa tatctagaaa taaaaaaagc actaagttga taagatgtgt
     4381 aagtcagatc aacttagtgc ctttactttg aaaaaaaata attttctcaa taatacttag
     4441 tactaactgt taacgaatat tatacttttt aaataatatt ctaaatacta atgacaatat
     4501 agtaatgcac gaaccaataa taaaaagatt aaagctattg ttaggattta atgccaccga
     4561 gaaaaagcca gtcccaatag gcataaaaag aatagttatt gtaaagataa taccaaacac
     4621 tctccctaaa aaatcgttat caacatcttt ctggacaaga gaaaaaaatt gaatattaaa
     4681 gatggaaaga aataaactga ataaagctgg tgacaaagct aaaataattg cattatgaaa
     4741 cataatataa aatgggggag ctaacattaa catcagtcct gataaagata gaaacaatat
     4801 caatctcata cttgataatt ctttattcac aaaaccactt aatattgccc caataaatcc
     4861 accaattgct tctgctgtta aaaaagtgcc ataaagtcca gttgaaattt ctccaaacat
     4921 ttgattacta tagggcaaca ataaattata agcggctaga aaaaaattaa caagtgcaga
     4981 aagaacggta ataataaaga tggacttatg gctgtaaaca tatttaaatc ctatttttaa
     5041 atcgttaaat atttctctga ttgtcacctt ctcctttatc accacttcgt cattaacagg
     5101 taaaataaag gaaattagta aagcagctat tagaaatgat agtccatcca atagtaatac
     5161 accgtgtata ccaagtagct tatataagaa aattgctacc ataggaactg ttactttgat
     5221 tacagtactt gttgtctcta gcaatgaatt aagttgtgat atactatctt ttttaacaat
     5281 ttcttttgta aatgctttat aggatggact agaaaaagca ctcataaatg ccaagataac
     5341 attagttagt acaatggcat aaaccaacca ttgctctttt gttaggaacg agagtactaa
     5401 gcaagctgtg ccacataaga tatttgtagt aataataatt tttttccgct tgaaactgtc
     5461 tgcaatgact ccaccaaata aattaaatag aacacctatg acactttcta gagactgata
     5521 tactgcaact aatgatagag acgctggatt aagcccagca agaaaagtat tgttagcaaa
     5581 atcaaacata acatcgccaa ttcgatatat aactttacta t
//
LOCUS       DAUDYX010000076         5439 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.077, whole genome shotgun sequence.
ACCESSION   DAUDYX010000076 DAUDYX010000000
VERSION     DAUDYX010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.077"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            86..643
                     /gene="lepB"
                     /locus_tag="VHD02_001761"
     CDS             86..643
                     /gene="lepB"
                     /locus_tag="VHD02_001761"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEQ5498349.1"
                     /translation="MVKRDFIRNILLLLIVIIGAILLRIFVFSTFKVSPETANTYLKS
                     GDLVTIKKNIQPKYKDFVVYRVGKKDYVSRVIAVEGDSVTYMDDIFYLNNMVESQAYL
                     EKMKAHYLNHAPFGTLYTDDFTVATITADKYQKVPKGKYLLLNDNRKNTNDSRRFGLI
                     NASQIKGLVTFRVLPLSDFGFVEVE"
     gene            819..2633
                     /gene="glmS"
                     /locus_tag="VHD02_001762"
     CDS             819..2633
                     /gene="glmS"
                     /locus_tag="VHD02_001762"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEQ5498350.1"
                     /translation="MCGIVGVVGNRNATDILMQGLEKLEYRGYDSAGIFVANAHQTNL
                     IKSVGRIADLRAKIGIDVAGSTGIGHTRWATHGQSTEDNAHPHTSQTGRFVLVHNGVI
                     ENYLHIKTEFLAGHDFKGQTDTEIAVHLIGKFVEEDKLSVLEAFKKALSIIEGSYAFA
                     LMDSQATDTIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETSEFMEIHDKELVILTKD
                     KVIVTDYDGKELIRDSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRQLISTYADETG
                     NVQVDPAIITSIQEADRLYILAAGTSYHAGFATKNMLEQLTDTPVELGVASEWGYHMP
                     LLSKKPMFILLSQSGETADSRQVLVKANAMGIPSLTVTNVPGSTLSREATYTMLIHAG
                     PEIAVASTKAYTAQIAALAFLAKAVGEANGKQEALDFNLVHELSLVAQSIEATLSEKD
                     LVAEKVQALLATTRNAFYIGRGNDYYVAMEAALKLKEISYIQCEGFAAGELKHGTISL
                     IEEDTPVIALISSSQLVSSHTRGNIQEVAARGAHVLTVVEEGLDREGDDIIVNKVHSF
                     LAPIAMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
     gene            2815..3150
                     /locus_tag="VHD02_001763"
     CDS             2815..3150
                     /locus_tag="VHD02_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEQ5498351.1"
                     /translation="MSLPNCLQCQSEYVYEDGLLLICPMCAFEWTPGEEATEEEGLVV
                     LDSNGVRLSDGDTITVVKDLKVKGAPKDLKQGTRVKNIRLVEGDHNIDCKIDGFGAMK
                     LKSEFVKKI"
     gene            3257..3898
                     /locus_tag="VHD02_001764"
     CDS             3257..3898
                     /locus_tag="VHD02_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ5498352.1"
                     /translation="MTYIQQVLPSLLDGALVTLQVFFIVIILSIPLGTILAFLMKIPF
                     KPLQWFLTLYVWMMRGTPLLLQLIFFYYVLPSVGISFDRMPAAILAFTLNYAAYFAEI
                     FRGGIEAIPKGQYEAAKVLKLKPLQTIRYIILPQVFKIVLPSVFNEVINLVKDSSLVY
                     VLGVGDLLLASKTAANRDATLAPMFIAGLIYLLLIGLVTIISKQVEKRFNYYQ"
     gene            3908..4537
                     /locus_tag="VHD02_001765"
     CDS             3908..4537
                     /locus_tag="VHD02_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ5498353.1"
                     /translation="MLELKNISKQFGQKTIFDGFNLTVQDGEVLSLVGPSGGGKTTLL
                     RMLAGLESIDSGQVFYNGEDVGIDHLENRNLLGFVFQDFQLFPHLTVLDNLTLSPTIT
                     MGKQKADAKEKALDLLARLGLKEHAQVYPYSLSGGQKQRVALARAMMIAPQIIGYDEP
                     TSALDPELRQTVEALIVQNREMGITQIVVTHDLVFAEAISDRIIRVNPK"
     gene            4553..5389
                     /locus_tag="VHD02_001766"
     CDS             4553..5389
                     /locus_tag="VHD02_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ5498354.1"
                     /translation="MIIKKRTVAILAIASSFFLVACQATKSLKSGDAWGVYQKQKSIT
                     VGFDNTFVPMGYKDESGKCKGFDIDLAKEVFHQYGLKVNFQAINWDMKEAELNNGKID
                     VIWNGYSITKERQDKVAFTDSYMRNEQIIVVKKRSDIKTISDMKHKVLGAQSASSGYD
                     SLLRTPKLLKDFIKNKDANQYETFTQAFIDLKSDRIDGILIDKVYANYYLAKEGQLEN
                     YRMIPTTFENEAFSVGLRKEDKTLQAKINRAFRVLYQNGKFQAISEKWFGDDVATANI
                     KS"
BASE COUNT         1692 a          874 c         1142 g         1731 t
ORIGIN      
        1 aacgtttatt ggtattgatc aaaaattgtt tacttttttt ctagcttaga acatggtata
       61 ataagacaat agactaggag ttaatatggt aaagcgagat tttataagaa atatattatt
      121 gttattaatt gtgattattg gggctatttt gttgagaatc tttgtttttt caacttttaa
      181 agtttctcca gaaacagcta atacttattt aaagagtggt gatttagtca caatcaaaaa
      241 aaatattcag cccaaatata aagattttgt ggtttataga gttggaaaaa aagattatgt
      301 cagtcgagtc attgctgttg aaggcgatag cgtgacttat atggacgata ttttttacct
      361 caataatatg gtagagtcac aggcttacct tgaaaagatg aaagcacatt acttgaatca
      421 tgcaccgttt ggcacattgt atacagatga ttttacagtt gctaccatca cagctgataa
      481 gtatcaaaaa gttcctaagg ggaagtatct tcttttgaat gataaccgga aaaatacgaa
      541 tgacagtcgt cgatttgggt taataaatgc ctcgcagatt aaaggtttag tgacctttag
      601 agttttgcct ctcagcgatt ttggatttgt agaagtagag tagttgtcag gctactctac
      661 tttttttata attagactat accaatttta tgtttgacaa gacattgtgg atattatata
      721 ctgtaaacat aatgttttta tcggtataat tagactatac caattattga aattatgaga
      781 gaaagtagca agttaacgaa cttgttaagg taattaagat gtgtggaatt gttggagttg
      841 ttggaaatcg caatgcaacg gatattttaa tgcaaggcct tgaaaagctt gaataccggg
      901 gttatgattc agcaggaatt tttgtggcta atgcccatca aacaaacttg attaaatcag
      961 tggggcggat tgctgatttg cgtgccaaga ttggcattga tgttgctggt tcaacaggga
     1021 ttggtcacac ccgttgggca acgcatggcc aatcaacaga ggataatgcc catcctcaca
     1081 cgtcacaaac tggacgtttt gtacttgttc ataatggtgt gattgaaaat taccttcaca
     1141 ttaaaacaga gttcctagct ggacatgatt ttaaggggca gacagatact gagattgcag
     1201 tacacttgat tggaaaattt gtggaagaag acaagttgtc agtactggaa gcttttaaaa
     1261 aagctttaag cattattgaa ggttcctacg cctttgcgtt aatggatagc caagcaactg
     1321 atactattta tgtggctaaa aacaagtctc cattgttgat tggacttggt gaaggttaca
     1381 acatggtttg ttcagatgcc atggccatga ttcgtgaaac cagtgaattt atggaaattc
     1441 atgataagga gctagttatt ttaaccaaag ataaggtaat tgttacagac tacgatggta
     1501 aagagctgat acgagattcc tacactgctg aattagactt atctgatatt ggcaaaggga
     1561 cttatccttt ctatatgctg aaagaaattg atgagcaacc aaccgtaatg cgtcaattaa
     1621 tttcaactta tgcagatgaa actggtaacg tacaggttga tccagctatc attacctcta
     1681 tccaagaggc tgaccgtctt tatattttag cggcagggac ttcctaccat gctggttttg
     1741 caacaaaaaa tatgcttgag caattgacag atacaccagt tgagttgggc gtggcttctg
     1801 agtggggtta ccacatgcct ctgcttagca agaaaccaat gtttattcta ctaagccaat
     1861 caggagaaac cgcagatagt cgtcaagttt tagtaaaggc aaatgctatg ggcattccga
     1921 gtttgacagt aactaacgtt ccaggatcaa ccttatcacg tgaagcaaca tacaccatgt
     1981 tgattcatgc tggacctgaa attgctgttg cgtctacaaa agcttacact gcacaaattg
     2041 ctgcccttgc ctttttggct aaggcagttg gtgaggcaaa tggtaagcaa gaagctcttg
     2101 actttaactt ggtacatgag ttgtcattgg ttgcccaatc tattgaggcg actttgtctg
     2161 aaaaagatct cgtggcagaa aaggttcaag ctttgctagc tactactcgt aatgcttttt
     2221 acatcgggcg tggcaatgat tattacgttg cgatggaagc tgctttgaaa ttaaaagaga
     2281 tttcttatat tcaatgcgaa ggctttgcgg ctggtgaatt gaaacatggc accatttcat
     2341 tgattgagga ggacacgcca gtaatcgctt taatatcgtc tagtcagttg gtttcctctc
     2401 atacgcgtgg taatattcaa gaagttgctg cccgtggggc tcatgtttta acagttgtgg
     2461 aagaagggct tgaccgtgag ggagatgaca ttattgtcaa taaggttcat tctttcctag
     2521 ccccgattgc tatggtcatt ccaactcaac tgattgctta ctacgcttca ttacaacgtg
     2581 gacttgatgt tgataagcca cgtaatttgg ctaaagctgt aacagtagaa taaatgatta
     2641 taatggataa ggcaaccatc tgttaggtgg ttgctttttg tttttaggag aatagcagag
     2701 cttagaaaat ggccttgaga agaatagtca tcttctgatg agaacgtatt catcttgtca
     2761 aatcattgat tctaaagtaa aatggtaaca ttaagaaatg tttaggagtg taagatgtca
     2821 ttaccaaatt gtttgcaatg ccagtctgaa tatgtttacg aagatggtct tttattaatc
     2881 tgtccaatgt gtgcttttga atggacacca ggcgaagaag cgactgaaga agaaggactt
     2941 gttgttttgg atagcaatgg tgtccgactg tctgatggtg ataccattac tgtggttaaa
     3001 gatttgaaag taaaaggcgc tccaaaagac ctgaaacaag gaactcgagt taaaaatatt
     3061 cgcctggtgg aaggtgatca taacattgat tgtaagattg atgggtttgg cgcgatgaaa
     3121 ttgaaatcag aatttgtcaa gaaaatctaa taataatacg caaccagctg atctggttgt
     3181 atttttataa aaaaatctct ccccaaatac gttcaaattt ggtataatag taaaatattt
     3241 agttttagga ggcaacatga cctatattca gcaagtttta cccagcttat tagatggtgc
     3301 cttggtgacc ttacaagtat tctttattgt tatcattctt tctataccct tagggactat
     3361 tttagctttc ttgatgaaga ttccctttaa accgctacag tggtttttga ccctatacgt
     3421 gtggatgatg cgagggacac cattactact tcaattgatt tttttctatt atgttttgcc
     3481 aagtgtgggg attagttttg atcgaatgcc agctgctatt ttggcgttta ctttgaatta
     3541 tgctgcctac tttgctgaaa tttttagagg tgggattgag gctattccaa aaggtcaata
     3601 tgaagcagct aaagtattaa agttaaaacc tcttcaaacc attcgttata ttattttgcc
     3661 tcaagtgttt aaaattgtgt taccaagtgt tttcaatgaa gtcattaatt tggtcaaaga
     3721 ttcttctctt gtctatgtac tcggtgtagg agatctttta ttagcaagta agacggcagc
     3781 caatagggat gcaaccttag cccctatgtt tatcgctggt cttatctatt tgcttttaat
     3841 tggactggtc acgattattt caaaacaagt tgaaaaacgg tttaattatt atcagtaagg
     3901 aggcttcatg ttagaactca aaaatatttc caaacagttt ggtcaaaaaa ccatttttga
     3961 tggctttaat ctaaccgttc aagacggaga agtgctctct ttggtaggcc catcaggtgg
     4021 cggtaagaca acactcttac ggatgttggc tggtcttgaa tctattgatt caggtcaagt
     4081 cttttacaat ggcgaagatg ttggtattga tcatttggaa aatcgtaact tgcttggatt
     4141 tgttttccaa gattttcaat tgtttcctca tcttaccgtt ttagacaacc taacgctgtc
     4201 tccaacaatt acaatgggca agcaaaaggc tgacgctaaa gaaaaggctt tggatttact
     4261 ggcacgttta ggattaaaag aacatgccca agtttatccc tattcgttat ctggaggtca
     4321 aaaacaacgg gttgctttgg caagagccat gatgattgcc cctcaaatta tcggttacga
     4381 tgaaccgacc agtgctctag atcctgaatt acgtcagacg gtagaagcct tgattgttca
     4441 aaaccgtgaa atgggaataa ctcaaattgt agtcactcac gatcttgttt ttgccgaagc
     4501 catctcagat cgtattatta gggttaatcc taaatagaag aggacccaaa agatgattat
     4561 aaaaaaaaga accgtagcaa ttttagccat agctagtagc tttttcttgg tagcttgtca
     4621 agctactaaa agtcttaaat caggagatgc ttggggagtt taccaaaagc aaaaaagtat
     4681 tacagttggt tttgacaata cgtttgttcc tatgggctat aaggatgaaa gcggcaaatg
     4741 caaaggtttt gatattgatt tggctaaaga agtttttcac caatatggac tcaaggttaa
     4801 ctttcaagct attaattggg acatgaaaga agcagaacta aacaatggta aaattgatgt
     4861 aatctggaat ggttattcaa taactaagga gcgtcaggat aaggttgcct ttactgattc
     4921 ttacatgaga aatgaacaaa ttattgttgt caaaaaaaga tctgatatta aaacaatatc
     4981 agatatgaaa cataaagtgt taggagcaca atcagcttca tcaggttatg actccttgtt
     5041 aagaactcct aaactgctga aagattttat taaaaataaa gacgctaatc aatatgaaac
     5101 ctttacacaa gcttttattg atttaaaatc agatcgtatc gatggaatat tgattgacaa
     5161 agtatatgcc aattactatt tagcaaaaga agggcaattg gagaattatc ggatgatccc
     5221 aacgaccttt gaaaatgaag cattttcggt tggacttaga aaagaagaca aaacgttgca
     5281 agcaaaaatt aatcgtgctt tcagggtgct ttatcaaaat ggcaaatttc aagctatttc
     5341 tgagaaatgg tttggagatg atgttgccac tgccaatatt aaatcttaaa aaggacactt
     5401 gtcctttttt tgtttaacag gtgagatcta gcagacaaa
//
LOCUS       DAUDYX010000077         5133 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.078, whole genome shotgun sequence.
ACCESSION   DAUDYX010000077 DAUDYX010000000
VERSION     DAUDYX010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.078"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(102..635)
                     /locus_tag="VHD02_001767"
     CDS             complement(102..635)
                     /locus_tag="VHD02_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ5498355.1"
                     /translation="MVNRKENTKQAILKAMVMLLKTESFDDITTVKLSKRAGISRSSF
                     YTHYKDKYEMIDYYQQTFFHKLEYIFEKKYQNKEQAFLEVFEFLQREQLLSSLLSANG
                     TKEIQAFIINKVRLLITTDLQDKFRTEELSQTEKEYRSIYLAHAFFGVCQSWIAKGKK
                     ESPQEMTQFVLKMLTST"
     gene            770..3043
                     /locus_tag="VHD02_001768"
     CDS             770..3043
                     /locus_tag="VHD02_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEQ5498356.1"
                     /translation="MLEELKTLIKNPKLMITMIGVALVPALYNLSFLGSMWDPYGRVN
                     DLPIAVVNHDKPAKRADKSLTIGNDMVDKMSKSKDLEYHFVSAKQAQEGLKEGDYYMV
                     ITLPEDLSQRAATLLNPEPQKLTIRYQTSKGHGMVAAKMGETAMAKLKESVSQNITKT
                     YTSAVFSSMTDLQSGLKEASAGSQALASGAKTAQAGSQTLSTNLAALTGASQQFQQGT
                     GRLTSGLTTYTDGVNQVKNGLGTLSTDIPNYLNGVSRLSQGASQLNQGLSQLTQATTL
                     SDEKAKGIQSLIVGLPVLNQGIQQLNTELSTLHPPNLNADELGNSLGAIAQAAKQVIA
                     EETAAQNEQLSALQATSVYQSLTAEQQGELAAALSQSDKSQTVSAAQTILSSVQTLST
                     GLQTLSQGNSLAQLDQLKAAVSQIASQSNQALPGASSALSELSMGLSKVNGSLNQQVL
                     PGSNQLTTGLAQLNRYNTVIGSGVIKLSEGANALSSKSGELLDGSHQLSEGATKLADS
                     SSQLSQGGHQLTSGLTELSTGLSTLNGSLAKASQQLSLVSVTDKNAKAVAKPLVLNEK
                     DKDGVKTNGIGMAPYMIAVSLMVVALSTNVIFANSLSGRPVKDKWDWAKQKFVINGFI
                     STMGSIVLYLAIQLLGFEARYGMETLGFIMLSGWTFMALVTALVGWDDRYGSFASLVM
                     LLLQVGSSGGSYPIELSGAFFQKLHPFLPMTYVVSGLRQTISLSGHIGVEVKVLTGFL
                     LAFMVLALLIYRPKKTV"
     gene            complement(3159..4232)
                     /locus_tag="VHD02_001769"
     CDS             complement(3159..4232)
                     /locus_tag="VHD02_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEQ5498357.1"
                     /translation="MKNISRKCFMTSVVCIVLGAILLGAGYATGGLQDIKHQTAPKKV
                     IQTFDHVTALDIDTNFSTITVETGPVKKPTVTYYTHSKFIDPLEIKIENETLHISEKP
                     RDVIITGGIELIGFGLNKSDRNHNYRTTTITLPKGTSLKEFKGSSMPQATISDLTVQD
                     LEFSGGLTLLNTRIKKGKVFGTFDATDSLLANLELKADYSLSSLTNSSLENGTISLGH
                     GQLTTKNTNLKSVTIQSLSPGGVEAEETSLENVTFTVTSEQENEETNDNGAIFTAHAL
                     TLKGTNTISGGNVAVDITLTDPKTTAYKTKSEDGKISLGSQLTPAKIGKESTSDVISY
                     VAENKAATGNLTVNLNKGDITIK"
     gene            complement(4229..4816)
                     /locus_tag="VHD02_001770"
     CDS             complement(4229..4816)
                     /locus_tag="VHD02_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEQ5498358.1"
                     /translation="MTRTEYLAELDKYLRKLPREDYHEAMEYYIEYFDEAGPDKENQV
                     IEDLGSPKEAASEIIATVLGKHMASPEKTPEKRATIVGLAILALFAAPIALPVLLALI
                     LFIIACLMAGITAIVAAYVFGLVGVLVAGITLFESLTLLGSSLPAQAMGIGSALLSFG
                     GGILIWIIATALLRFSGRAFVAFIKWISHKKGAQA"
     gene            complement(4803..5129)
                     /locus_tag="VHD02_001771"
     CDS             complement(4803..5129)
                     /locus_tag="VHD02_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEQ5498359.1"
                     /translation="MYFPVSATLIEFLILAIVEKNDSYGYDISQTIKLVANIKESTLY
                     PILKKLEKAGFLTTYSQEHQGRKRKYYAVTSSGRAQLIFLKKEWQSYKFALDGIIEGS
                     LRHDKD"
BASE COUNT         1528 a          974 c         1002 g         1629 t
ORIGIN      
        1 ataacaaatc tattctgatc ccttcatttt actatggctt ctaaaaaagt tatcactaag
       61 gctgatgact tgtaaaatga tactttcgta agggttacta tttaggtact cgtaagcatt
      121 ttgagaacga attgtgtcat ttcttgaggt gattcttttt tacctttagc aatccaactt
      181 tgacagacac caaaaaaagc atgggctaag tagatacttc ggtattcttt ttccgtttga
      241 gataactctt cggtgcggaa cttgtcctgt aaatcagtag tgatgagtag gcgaacttta
      301 ttgatgataa aggcctgtat ttctttggta ccgttagcag acaaaaggga agagagaagc
      361 tgttctcttt ggagaaattc aaatacttca aggaaggctt gttctttatt ttgatatttt
      421 ttctcaaaaa tatattctaa tttgtgaaaa aaggtttgct gataatagtc aatcatttca
      481 tatttatctt tatagtgtgt gtaaaaacta gagcgactaa ttccagctct tttagataat
      541 ttaactgtgg taatatcgtc aaaactttcg gtttttagta acataaccat tgcttttaaa
      601 attgcttgct tcgtattttc tttacggttg accatctacc atctctcctt ttgaacactt
      661 ttagacataa tgtccaaaag tgaatttttt tctcttgtaa atttaaattt aaattgtata
      721 atatatttta tcataatttt tgaacagtat gtccatttta ggagaaaata tgttagaaga
      781 attgaaaaca cttattaaaa atccaaaatt aatgattaca atgattggtg tggccctagt
      841 gcctgcctta tataatttat cctttctagg ctcaatgtgg gatccttatg gtcgggtcaa
      901 tgaccttccc attgctgttg ttaatcatga taagcctgca aagagagctg ataagtcatt
      961 gacaattggg aatgatatgg tggacaagat gtctaaaagt aaagatttag agtatcattt
     1021 tgtctcagct aagcaagctc aagagggact taaagaaggt gattattata tggttattac
     1081 tttacccgaa gatctttctc aacgggcagc aaccttatta aatcccgaac cccaaaaatt
     1141 aactatccgt taccaaacga gtaaaggaca tggaatggtc gctgctaaga tgggggaaac
     1201 agcgatggct aagctgaaag agtctgtttc gcaaaacatt acgaagactt atacctcagc
     1261 agtttttagc agtatgacag acctccaatc aggattaaaa gaagcctcag ctggaagtca
     1321 agcacttgct tcaggagcga agaccgctca ggcggggagt caaacacttt cgacgaactt
     1381 agcagcctta acgggtgcta gccaacagtt tcaacaaggt actggtcgat tgacatcagg
     1441 tttgactacc tatacagatg gtgtcaacca agtcaagaat gggttaggaa cattatcaac
     1501 ggacatcccc aattatctga acggggtttc taggttatcc cagggagctt ctcagcttaa
     1561 tcagggtctt tcacagttga cacaagcaac aacactttct gacgagaaag ctaagggaat
     1621 tcaatcctta attgtaggac taccagtcct aaatcaaggc attcagcaac taaatacaga
     1681 gctatcaaca ttgcaccccc ctaaccttaa tgctgatgag ttaggtaata gcttaggagc
     1741 tatcgctcaa gctgccaaac aagttattgc tgaagagact gccgctcaga atgaacaact
     1801 atcggctctc caagccacta gtgtttatca atcattaact gctgaacaac aaggagagtt
     1861 agctgcggcc ctcagtcaat ctgataaaag tcaaaccgta tctgcagccc aaactatttt
     1921 aagttctgtt caaacactgt caacaggttt acaaactctg tcccaaggaa atagcttagc
     1981 tcaattagac cagcttaaag cagctgtttc acagattgcg agtcagtcta accaggcttt
     2041 acctggagcg agttctgcat tatctgagtt gtcaatgggg ttatcaaagg taaatggtag
     2101 cttaaatcaa caagttctac caggaagtaa tcaattgaca acaggattag cacaattaaa
     2161 caggtataat actgtcattg gttctggggt aataaaactc tcagaaggtg ccaatgcctt
     2221 gtcatccaag tccggagaat tactagatgg tagccatcaa ttatcagagg gtgctactaa
     2281 actagctgat agtagttctc aattgagtca gggtggtcat caattaacga gcggattgac
     2341 tgaattatca acaggattat caaccttaaa tggttcctta gccaaagcct ctcagcagtt
     2401 atcgcttgtt tctgtgactg ataaaaatgc taaagctgtc gcaaaacctc ttgtgttaaa
     2461 tgagaaagac aaagatggtg ttaagacgaa tgggatcggg atggcacctt atatgattgc
     2521 tgtttctcta atggttgtgg ccctttcaac caacgtcatt tttgctaatt ctttatctgg
     2581 tcgtccggtc aaagataaat gggattgggc taaacaaaaa tttgttatta atggttttat
     2641 ttcgactatg ggatccattg ttctctactt agctattcaa ttattagggt ttgaagcccg
     2701 ttatggtatg gaaaccttag gatttattat gctaagtggt tggacgttta tggctcttgt
     2761 cacagctttg gtcggttggg atgatcgata tggctctttt gcttctttgg tcatgttatt
     2821 acttcaggtt ggctcttcag gtggctctta ccccattgag ttaagtggag cattcttcca
     2881 aaagttacat cctttcttac caatgactta tgtggtatct ggtttacgac aaaccatttc
     2941 attatcaggt catattggag tagaagtgaa agtcttaact ggtttcttac tggcatttat
     3001 ggtattagca ctactcattt atcgtcccaa gaaaacagtc taaataaaat aacctcaaaa
     3061 aaaacagcat ctaaggcagt tacactagat gctgtttttt gtcttatttt gacaataact
     3121 tgacaatttc tgtcaagttt tttattgtct tgttaagatc atttgatagt aatgtctccc
     3181 ttattgagat taaccgttaa atttccagta gctgctttat tctcagccac ataagaaata
     3241 acatctgaag ttgattcctt accaatttta gctggtgtca gctgtgatcc aagtgagatt
     3301 ttaccatctt cgctttttgt cttataggcg gtcgtctttg gatcggtcaa agttatgtca
     3361 acagcaacat taccaccact aatagtatta gtgcctttaa gggtaagagc atgagcagtg
     3421 aatatagcac cattatcatt ggtttcctcg ttttcttgct cactagtaac agtgaaagtc
     3481 acattttcaa ggcttgtttc ttcggcttca accccaccag gagatagaga ttggatggtg
     3541 acagatttaa gattagtatt ttttgtggtt aattgtccat gccctaagct aatcgtccca
     3601 ttttctaaac tagaattcgt aaggcttgac aacgaatagt cggctttcaa ctcaagattg
     3661 gctaacaagc tatcagtagc gtcaaatgtc ccaaaaacct tccctttctt tattctagta
     3721 tttagaagtg tcagaccacc actgaactca aggtcttgaa ccgttaaatc cgatatagtg
     3781 gcttgaggca tgcttgagcc tttgaactct ttaagactgg taccttttgg aagcgttatg
     3841 gtagtggttc ggtagttgtg gttgcggtca cttttattta atccaaaccc gatcagttca
     3901 atacctccgg tgatgatgac atctctgggt ttttcagaaa tatgaagtgt ctcgttctct
     3961 attttaattt ctaaaggatc aataaattta gaatgtgtgt aataggtaac ggttggtttt
     4021 ttgacaggtc ctgtttctac tgtaatagtt gaaaaattgg tatcaatatc aagagctgtt
     4081 acgtgatcaa atgtttggat aaccttttta ggggctgttt ggtgtttaat gtcttgaagc
     4141 ccgccagttg cataaccagc tcctaaaaga atggctccta aaacaataca cacgacactg
     4201 gtcataaaac atttgcgtga gatatttttc atgcttgagc tcctttcttg tgactaatcc
     4261 atttaataaa ggcaacgaag gcacgtcctg aaaaacgcag gagtgcagtg gcaataatcc
     4321 aaatgagaat accaccacca aagctcagca aggcgctacc aattcccata gcctgagcag
     4381 gaagagatga accaagtaaa gtcaaacttt caaagagggt gattcctgca acaagtaccc
     4441 ctacaaggcc aaaaacataa gcagcaacga tagctgttat tccagccatg aggcaagcaa
     4501 taataaagag aattaaagcc aataaaaccg gtaaagcgat aggcgcagca aagagtgcta
     4561 aaatagctag cccgacgata gttgctcgct tttcaggtgt tttttcagga cttgccatgt
     4621 gttttcctag aacagttgcg atgatttcac tagcggcctc tttggggctt cctaaatcct
     4681 cgataacttg attttcttta tcaggaccag cttcatcaaa atattcaata taatattcca
     4741 tcgcttcatg gtagtcttct ctaggtagtt tgcgcaagta tttatctagt tctgctagat
     4801 actcagtcct tgtcatgtct taagctccct tcaataatcc cgtctaaggc aaatttatag
     4861 ctctgccatt ctttttttag aaaaatcagc tgtgctctcc ccgaagaagt gacagcataa
     4921 tatttacgct ttcgtccctg atgttcttga ctataagttg tcaaaaaacc tgccttctcc
     4981 aattttttaa gaataggata gagggtggat tctttgatat tagccactag tttaatggtt
     5041 tgactaatat cgtaaccgta agaatcattt ttttcgacaa tggctaaaat cagaaattca
     5101 atcaaggtag ccgatactgg aaaatacatt tta
//
LOCUS       DAUDYX010000078         5058 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.079, whole genome shotgun sequence.
ACCESSION   DAUDYX010000078 DAUDYX010000000
VERSION     DAUDYX010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.079"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            5..1553
                     /locus_tag="VHD02_001772"
     rRNA            5..1553
                     /locus_tag="VHD02_001772"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1605..1677
                     /locus_tag="VHD02_001773"
     tRNA            1605..1677
                     /locus_tag="VHD02_001773"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Ala,seq:tgc)
     gene            1976..4878
                     /locus_tag="VHD02_001774"
     rRNA            1976..4878
                     /locus_tag="VHD02_001774"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            4964..>5058
                     /gene="rrf"
                     /locus_tag="VHD02_001775"
     rRNA            4964..>5058
                     /gene="rrf"
                     /locus_tag="VHD02_001775"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1422 a         1046 c         1456 g         1134 t
ORIGIN      
        1 gcaaattaaa gagagtttga tcctggctca ggacgaacgc tggcggcgtg cctaatacat
       61 gcaagtagaa cgctgagaac tggtgcttgc accggttcaa ggagttgcga acgggtgagt
      121 aacgcgtagg taacctacct catagcgggg gataactatt ggaaacgata gctaataccg
      181 cataagagag actaacgcat gttagtaatt taaaaggggc aattgctcca ctatgagatg
      241 gacctgcgtt gtattagcta gttggtgagg taaaggctca ccaaggcgac gatacatagc
      301 cgacctgaga gggtgatcgg ccacactggg actgagacac ggcccagact cctacgggag
      361 gcagcagtag ggaatcttcg gcaatggggg caaccctgac cgagcaacgc cgcgtgagtg
      421 aagaaggttt tcggatcgta aagctctgtt gttagagaag aatgatggtg ggagtggaaa
      481 atccaccaag tgacggtaac taaccagaaa gggacggcta actacgtgcc agcagccgcg
      541 gtaatacgta ggtcccgagc gttgtccgga tttattgggc gtaaagcgag cgcaggcggt
      601 tttttaagtc tgaagttaaa ggcattggct caaccaatgt acgctttgga aactggagaa
      661 cttgagtgca gaaggggaga gtggaattcc atgtgtagcg gtgaaatgcg tagatatatg
      721 gaggaacacc ggtggcgaaa gcggctctct ggtctgtaac tgacgctgag gctcgaaagc
      781 gtggggagca aacaggatta gataccctgg tagtccacgc cgtaaacgat gagtgctagg
      841 tgttaggccc tttccggggc ttagtgccgg agctaacgca ttaagcactc cgcctgggga
      901 gtacgaccgc aaggttgaaa ctcaaaggaa ttgacggggg cccgcacaag cggtggagca
      961 tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt cttgacatcc cgatgcccgc
     1021 tctagagata gagttttact tcggtacatc ggtgacaggt ggtgcatggt tgtcgtcagc
     1081 tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aacccctatt gttagttgcc
     1141 atcattaagt tgggcactct agcgagactg ccggtaataa accggaggaa ggtggggatg
     1201 acgtcaaatc atcatgcccc ttatgacctg ggctacacac gtgctacaat ggttggtaca
     1261 acgagtcgca agccggtgac ggcaagctaa tctcttaaag ccaatctcag ttcggattgt
     1321 aggctgcaac tcgcctacat gaagtcggaa tcgctagtaa tcgcggatca gcacgccgcg
     1381 gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccacgagagt ttgtaacacc
     1441 cgaagtcggt gaggtaacct attaggagcc agccgcctaa ggtgggatag atgattgggg
     1501 tgaagtcgta acaaggtagc cgtatcggaa ggtgcggctg gatcacctcc tttctaagga
     1561 aatggaacac gtttatcgtc ttatttagtt ttgagaggtc ttgtggggcc ttagctcagc
     1621 tgggagagcg cctgctttgc acgcaggagg tcagcggttc gatcccgcta ggctccatca
     1681 ggatacaatc ctactaaact taatacaagt gaagttgaac acgcaactca cttcctagga
     1741 aaatagacaa tcttcccttg tgtgcaaggc ccacatggtc agattcctaa ttttctacag
     1801 aagtttcgct aaagcgagcg ttgcttggta tcctatataa tagtccattg aaaattgaat
     1861 atctatatca aattccacga tctagaaata gattgtagaa aagtaacaag aaaataaacc
     1921 gaaaacgctg tgactattta ataagttttc tagtttaaaa aaactaggtt aataaggtta
     1981 agttaataag ggcgcacggt ggatgccttg gcactagaag ccgaagaagg acgtgacaaa
     2041 cgacgaaatg ctttggggag ctgtaagtaa gcgctgatcc agagatgtcc gaatggggga
     2101 acccactaac taatggttag tatccataac tgttaaggtt atgagaagga agacgcagtg
     2161 aactgaaaca tctaagtagc tgcaggaaga gaaagcaaac gcgattgcct tagtagcggc
     2221 gagcgaaacg gcaggagggc aaaccgaagt gtttacactt cggggttgta ggactgcgac
     2281 gtgggacttt aaaaggatag aagaattacc tgggaaggta agccaaagag agtaaaagcc
     2341 tcgtatttaa aattcttttg agccctagca gtatcctgag tacggcgaga cacgtgaaat
     2401 ctcgttggaa tctgggagga ccatctccca accctaaata ctctctagtg accgatagtg
     2461 aaccagtacc gtgagggaaa ggtgaaaagc accccgggag gggagtgaaa tagaacctga
     2521 aaccgtgtgc ctacaacaag ttcgagcccg ttaatgggtg agagcgtgcc ttttgtagaa
     2581 tgaaccggcg agttacgata tgatgcgagg ttaagttgaa gagacggagc cgtagggaaa
     2641 ccgagtctta atagggcgat ttagtatcat gttgtagacc cgaaaccatg tgacctaccc
     2701 atgagcaggt tgaaggtgag gtaaaactca ctggaggacc gaaccagggc acgttgaaaa
     2761 gtgcttggat gacttgtggg tagcggagaa attccaaacg aacttggaga tagctggttc
     2821 tctccgaaat agctttaggg ctagcgtcga tgttaagtct cttggaggta gagcactgtt
     2881 tgggtgaggg gtccatctcg gattaccaat ctcagataaa ctccgaatgc caacgagata
     2941 tcatcggcag tcagactgcg agtgctaaga tccgtagtcg aaagggaaac agcccagacc
     3001 accagctaag gtcccaaaat aactgttaag tggaaaagga tgtggggttg cacagacaac
     3061 taggatgtta gcttagaagc agctattcat tcaaagagtg cgtaatagct cactagtcga
     3121 gtgaccctgc gccgaaaatg taccggggct aaaacagttt accgaagctg tggatgacac
     3181 aaaagtgtca tggtaggaga gcgttctatg tgtgaagaag gtgtaccgtg aggagcgctg
     3241 gaacgcatag aagtgagaat gccggtatga gtagcgaaag acaggtgaga atcctgtcca
     3301 ccgtaagact aaggtttcca ggggaaggct cgtccgccct gggttagtcg ggacctaagg
     3361 agagaccgaa aggtgtatcc gatggccaac aggttgatat tcctgtacta gagtatatag
     3421 tgatggaggg acgcagtagg ctaactaaac cggacgattg gaagagtccg gctaagcagt
     3481 gaggtgtaag atgagtcaaa tgcttatctt tataacattg agctgtgatg gggagcgaat
     3541 tttagtagcg aagttagtga tgtcacactg ccaagaaaag cttctagcgt ttaatgatac
     3601 tctacccgta ccgcaaaccg acacaggtag tcgaggcgag tagcctcagg tgatcgagag
     3661 aactctcgtt aaggaactcg gcaaaatgac cccgtaactt cgggagaagg ggtgctgact
     3721 taggtcagcc gcagtgaata ggcccaagca actgtttatc aaaaacacag ctctctgcta
     3781 aatcgtaaga tgatgtatag ggggtgacgc ctgcccggtg ctggaaggtt aagaggaggg
     3841 tttagcgcaa gcgaagatct gaattgaagc cccagtaaac ggcggccgta actataacgg
     3901 tcctaaggta gcgaaattcc ttgtcgggta agttccgacc cgcacgaaag gcgtaatgat
     3961 ttgggcactg tctcaacgag agactcggtg aaattttagt acctgtgaag atgcaggtta
     4021 cccgcgacag gacggaaaga ccccatggag ctttactgca gtttgatatt gagtatctgt
     4081 accacatgta caggataggt aggagccatt gacttcggga cgccagtttc gaatgaggcg
     4141 ctgttgggat actacccttg tgttatggct actctaaccc agataggtta tccctatcgg
     4201 agacagtgtc tgacgggcag tttgactggg gcggtcgcct cctaaagagt aacggaggcg
     4261 cccaaaggtt ccctcagatt ggttggaaat caatcgcaga gtgtaaaggt ataagggagc
     4321 ttgactgcga gagctacaac tcgagcaggg acgaaagtcg ggcttagtga tccggtggta
     4381 ccgaatggaa gggccatcgc tcaacggata aaagctaccc tggggataac aggcttatct
     4441 cccccaagag ttcacatcga cggggaggtt tggcacctcg atgtcggctc gtcgcatcct
     4501 ggggctgtag tcggtcccaa gggttgggct gttcgcccat taaagcggca cgcgagctgg
     4561 gttcagaacg tcgtgagaca gttcggtccc tatccgtcgc gggcgtagga aatttgagag
     4621 gatctgctcc tagtacgaga ggaccagagt ggacttaccg ctggtgtacc agttgtcttg
     4681 ccaaaggcat cgctgggtag ctatgtaggg aagggataag cgctgaaagc atctaagtgc
     4741 gaagcccccc tcaagatgag atttcccatg attttatatc agtaagagcc ctgagagatg
     4801 atcaggtaga taggttagga gtgtaagtgt agcgatacat gtagcggact aatactaata
     4861 gctcgaggac ttatccaaaa agaaatattg acaacgttac ggattcttgt tagactatag
     4921 atattcaatt ttgagtgggt tatgaaaata gcacatttac aagttaagtg acgatagcct
     4981 aggagataca cctgtaccca tgccgaacac agcagttaag ccctagaacg cctgatgtag
     5041 ttgggggttg ccccctgt
//
LOCUS       DAUDYX010000079         4927 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.080, whole genome shotgun sequence.
ACCESSION   DAUDYX010000079 DAUDYX010000000
VERSION     DAUDYX010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.080"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(83..325)
                     /locus_tag="VHD02_001776"
     CDS             complement(83..325)
                     /locus_tag="VHD02_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017228.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEQ5498360.1"
                     /translation="MTRQEIFERLINLIQKQRSYLSVAITEQTHLKNDLAVDSIELVE
                     FIINVEDEFHLAIPDEDVEDMVFMGDVLDYLVQRLT"
     gene            complement(318..1325)
                     /gene="plsX"
                     /locus_tag="VHD02_001777"
     CDS             complement(318..1325)
                     /gene="plsX"
                     /locus_tag="VHD02_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEQ5498361.1"
                     /translation="MKRIAIDAMGGDNAPKAIVEGVNQAIEAFSDIEIQLYGDQTKIN
                     SYLIQSDRVAIIHTDEKIMSDDEPAKAVRRKKKASMVLAAKAVKEGKADAIISAGNTG
                     ALLAVGLFVVGRIKGVDRPGLLSTIPTVTGLGFDMLDLGANAENTAKHLHQYAILGSF
                     YAKNVRGIANPRVGLLNNGTEETKGDPLRKATYELLTADNTISFVGNVEARELMSGVA
                     DVIVSDGFTGNAVLKSIEGTAISIMGQLKQIINSGGIKTKIGASLLKSSLYEMKKTLD
                     YSSAGGAVLFGLKAPVVKSHGSSDVKAIFSTIKQVRTMLDTNVVGQLVEEFAKETQVN
                     D"
     gene            complement(1428..2183)
                     /gene="recO"
                     /locus_tag="VHD02_001778"
     CDS             complement(1428..2183)
                     /gene="recO"
                     /locus_tag="VHD02_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEQ5498362.1"
                     /translation="MQLTESLGIVLFNRNYREDDKLVKIFTEVAGKQMFFVKHISRSK
                     MSSIIQPLTIADFIFKLNDTGLSYVVDYSNVNTYRYINNDIFRLAYASYVLALADAAI
                     ADNESDSHLFTFLKKTLDLMEEGLDYEILTNIFEIQILDRFGISLNFHECAICHRTDL
                     PLDFSHRFSAVLCSEHYYKDNRRNHLDPNVIYLLSRFQKITFDDLRTISLNKDIKKKL
                     RQFIDELYHDYVGIKLKSKTFIDNLVKWGDIMK"
     gene            complement(2369..3331)
                     /locus_tag="VHD02_001779"
     CDS             complement(2369..3331)
                     /locus_tag="VHD02_001779"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEQ5498363.1"
                     /translation="MSYSDLKLFALSSNKELAEKVASAMGLQLGKSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAEKISVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRH
                     GLVGEDVVVVSPDHGGVTRARKLAQFLQTPIAIIDKRRSVDKMNTSEVMNIIGNVSGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQRSAIEKLIVL
                     DTIYLPKERLIDKIEQISIADLVAEAIIRIHEKRPLSPLFEMGN"
     gene            complement(3584..4780)
                     /locus_tag="VHD02_001780"
     CDS             complement(3584..4780)
                     /locus_tag="VHD02_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ5498364.1"
                     /translation="MKKRILSAVLVSGVTLGAATTVGAEDLSTKIAKQDSIISNLTTE
                     QKAAQNQVSALQAQVSSLQSEQDKLTARNTELEALSKRFEQEIKALTSQIVARNEKLK
                     NQARSAYKNNETSGYINALLNSKSISDVVNRLVAINRAVSANAKLLEQQKADKVSLEE
                     KQAANQTAINTIAANMAMAEENQNTLRTQQANLVAATANLALQLASATEDKANLVAQK
                     EAAEKAAAEALAQEQAAKVKAQEQAAQQAASVEAAKSAITPAPQATPAAQSSNAIEPA
                     ALTAPASPSARPQVSYSSVNNYPVGQCTWGAKSLAPWAGNNWGNGGQWAASAQAAGYS
                     VGSTPMVGAIAVWNDGGYGHVAVVVEVQSSSNIRVMESNYSGKQYIADHRGWFNPTGV
                     TFIYPH"
BASE COUNT         1558 a          998 c          759 g         1612 t
ORIGIN      
        1 atttacgttt ataagttttt ttaaaaaaag gttcgttttt aaagaataat aaaaagagct
       61 tacgctcttt ttattattct aactaagtca aacgctgaac taaataatct aaaacgtctc
      121 ccataaatac catatcctca acatcttcat caggaatagc aagatgaaat tcatcttcaa
      181 cattaataat aaattcaact agttcaatag agtcaaccgc taaatcattt tttaaatgtg
      241 tttgttctgt aatagctaca ctcaaatagc ttctttgctt ctgaattaag ttaatcagcc
      301 tttcaaaaat ctcctgtcta gtcatttact tgagtctcct tagcaaattc ttctactaat
      361 tgtccgacca cgttagtgtc tagcatcgta cgtacttgtt ttatcgttga aaaaatagct
      421 tttacatcac tagaaccatg agactttaca acaggtgcct ttaaaccaaa taaaactgca
      481 cctccagcac tagagtagtc aagagtcttc ttcatctcgt aaagactact tttaagtaga
      541 ctagctccaa ttttagtctt aataccgccg ctattaatta tctgtttcag ttgccccata
      601 atgctaatag ctgttccttc tattgatttc aacactgcgt ttcccgtgaa accatctgat
      661 acaataacat ctgcaacacc tgacataagt tctcgcgcct cgacattacc aacaaaagat
      721 attgtattat ctgctgttaa aagttcataa gtggccttac gaaggggatc tccttttgtt
      781 tcttctgtac cattgtttaa tagaccaaca cgtggattag caatcccacg tacattctta
      841 gcatagaaag agcctaaaat agcatattga tgtaaatgtt ttgccgtatt ttcagcatta
      901 gctccaagat ccaacatatc aaatcctaat ccggttactg tgggaatagt tgataataat
      961 cctggacgat caactccctt gatacgcccc acaacaaaca aacctacagc taaaagagca
     1021 cctgtattac ctgctgaaat aatggcatct gcttttcctt ctttcactgc cttagctgct
     1081 aaaaccattg aagccttctt tttacggcga acagcttttg caggttcatc atctgacata
     1141 attttttcat ctgtatgaat aatagcaaca cgatctgatt gaataagata ggagttaatc
     1201 tttgtctgat caccgtaaag ttgaatttcg atatctgaaa atgcttctat tgcttgatta
     1261 acaccctcaa caatagcttt aggagcgtta tctccaccca tagcatctat tgcaattctc
     1321 ttcatttcca ctcctttaat tatatcttgt gttcttacac aatctattaa tagtattttt
     1381 attatagcat attctgaaac caattcccat aaacgaaaga ggccctatca tttcataata
     1441 tctccccact taactaaatt atcaatgaat gttttacttt ttaatttgat tcctacatag
     1501 tcgtgataca actcatcaat gaactgacga agcttctttt tgatgtcttt attcaatgaa
     1561 atagttctca aatcatcaaa tgtgattttt tgaaatcgac tcaacaagta gataacattt
     1621 ggatctaaat gattacgtcg gttgtctttg taataatgtt cagaacaaag tacagctgaa
     1681 aaacgatggg aaaaatcaag cggtaaatca gtgcgatgac aaatggcaca ctcatgaaag
     1741 tttagactaa taccaaaacg atctaatatc tgaatttcaa aaatatttgt caaaatttca
     1801 taatctaggc cctcttccat caaatcaagt gtttttttca aaaacgtaaa caaatgcgaa
     1861 tctgattcat tatctgcaat cgcagcatca gctaatgcta agacataact agcataggct
     1921 aatcgaaaaa tatcattatt aatataccga taagtgttaa cgttactata gtcaacaaca
     1981 taagacaagc ctgtatcatt taacttgaaa ataaaatcag caatcgttag tggttgaatg
     2041 attgaggaca ttttggaacg actaatatgt ttcacgaaaa acatctgctt acctgctact
     2101 tcagtaaata ttttgactaa tttatcatct tctcgataat tcctattaaa aagaacgatg
     2161 cctagtgatt ctgttagttg catattacct cgtcgtattt ttctcttacc ttttatctta
     2221 gtttacaata aactgttgtt atctttctta ttatacccaa aatcctttag aattgcatag
     2281 ttcatcccac aataattaat accacattac ccttacattt tattcaagtc aaaaagcctg
     2341 agatctatta tctcaggctt cgttaagttt aattgcccat ctcaaataaa ggtgacaacg
     2401 gacgtttttc atgaatgcga ataatcgctt ctgcaaccaa atcagcaatt gaaatttgtt
     2461 caattttatc aataaggcgt tcttttggca gataaatagt atcaagaaca attagttttt
     2521 cgatagccga acgttgaata ttatcaagtg ctggtccaga aagaacggga tgcgtacatg
     2581 acgcatacac agctgttgcc cccgcttcag caagcgcatc tgctgcatgg caaatagtcc
     2641 ctgcggtatc aatcatatcg tcaatcaaga tacatttctt gcccgaaaca tttccgataa
     2701 tattcatcac ttcactggtg ttcatcttat ccacactacg acgcttatcg ataattgcaa
     2761 ttggtgtttg gaggaattgt gctaatttac gcgcacgagt cacaccacca tggtcaggac
     2821 taacaacgac aacatcttca cccactaaac catgacgatc aaaatagtct gcaattaaag
     2881 gtgcacccat caagtgatca actggaatat caaagaaccc ttgaatttga gcagcatgta
     2941 aatcaaccgt taataagcga tctacaccag caacttctaa catattagca actaattttg
     3001 aagtaatagg ttcacgagaa cgcgctttac ggtcttggcg tgcatagccg tagtaaggca
     3061 tcacaactga aattttttca gcactagcac gctttaaagc atcgaccata atcaaaattt
     3121 ccattaaatt gtcattgaca ggtgagctag ttgattgcaa aataaagaca tgatgaccac
     3181 gaattgattc ttcaatattt acttggattt caccatctga aaactggcga accgtagact
     3241 ttccaagttg aagacccatt gcagacgcaa ctttttctgc taattccttg tttgacgata
     3301 aagcaaacaa ttttaaatca gaataagaca ttttttcctc caaagtttta atcctatacc
     3361 attttaacgc ttttcaagtg aattatcaaa tgaaattgaa aaaccgttaa agaatgtatt
     3421 aaatatacct tttatcgact ttatatactt ctcttatatt atacataagc gattggccaa
     3481 acaactctct tataaattaa ccatatcgtc aatactctca gaactattcc acaataggag
     3541 taatcaaaaa ggttagaaca actgttctaa cctaaataat atgttagtgt ggataaataa
     3601 atgtaacacc tgttggatta aaccaaccac ggtggtcagc aatatactgt ttaccactgt
     3661 agtttgattc catcactcga atatttgatg aactttgaac ttcaacaact actgcgacgt
     3721 gaccataacc gccatcattc caaaccgcaa tagctcctac cataggtgta gatcctacag
     3781 agtagcccgc tgcttgagcg cttgcagccc attgtccacc gttaccccaa ttgtttcctg
     3841 cccatggagc taatgatttc gcaccccatg tacattgtcc tactgggtaa ttattaacag
     3901 aactatatga aacttgtggt cttgcagaag gagatgccgg agccgtgagt gcagctggtt
     3961 caatagcatt actactttgc gctgccggag tagcttgtgg tgctggagta atagcagatt
     4021 ttgctgcttc aacagatgct gcttgttgtg cagcctgttc ttgtgcctta actttagcag
     4081 cctgttcttg tgctaaggct tcagcagcag ctttttctgc agcttctttt tgagctacca
     4141 aattagcttt atcttcagta gcagatgcta attggagagc taaatttgca gttgcagcta
     4201 ccaaattagc ttgttgagta cgtaatgtat tttggttttc ttcagccatt gccatattag
     4261 cggcaatggt attaatagct gtttggttag cagcttgctt ttcttcaagg gaaactttat
     4321 cagctttttg ttgttctaac aatttagcgt tagcagagac agctctatta attgctacta
     4381 aacggtttac aacatcagaa attgatttag aattcaaaag tgcattaata taaccagaag
     4441 tttcattgtt tttataagca ctacgagctt gattttttaa tttttcatta cgagcaacaa
     4501 tttgacttgt tagagcctta atttcttgct caaatcgctt tgaaagcgcc tcaagttctg
     4561 tatttcttgc ggtcagttta tcttgttcag attgtagtga acttacttga gcctgtaacg
     4621 ctgaaacttg attctgtgca gctttttgct ctgtagtcag atttgagata atagaatctt
     4681 gcttagcaat cttagtactt aaatcctccg ctcctacagt tgtagctgct ccgagggtaa
     4741 caccacttac aagaactgct gataaaattc tttttttcat aataaaaaaa ctactccttt
     4801 tcgataagac atcttatatt ttaccaaaaa aaaaagtatt taatattacg caattgttac
     4861 agttatatgt aataaacaat aaaaatattt tagggattat cttataaaag taaagcagcc
     4921 aaaaaga
//
LOCUS       DAUDYX010000080         4631 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.081, whole genome shotgun sequence.
ACCESSION   DAUDYX010000080 DAUDYX010000000
VERSION     DAUDYX010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.081"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            65..1387
                     /gene="ska"
                     /locus_tag="VHD02_001781"
     CDS             65..1387
                     /gene="ska"
                     /locus_tag="VHD02_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023611186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptokinase"
                     /protein_id="HEQ5498365.1"
                     /translation="MKNYLSFGMFALLFALTFGTVKPVQAIAGPEWLLDRPSVNNSQL
                     VVSVAGTVEGTNQEISLKFFEIDLTSRPAHGGKTEQGLSPKSKPFATNSSAMPHKLEK
                     ADLLKAIQEQLIANVHSNDGYFEVIDFASDATITDRNGKVYFADRDDSVTLPTQPVQE
                     FLLSGHVRVRPYQPKAVHNSAERVNVNYEVSFVSETGNLDFTPSLKERYHLTTLAVGD
                     SLSSQELAAIAQFILSKEHPDYIITKRDSSIVTHDNDIFRTILPMDQEFTYHIKDREQ
                     AYKANSKTGIVEKTNNTDLISEKYYVLKKGEEPYDPFDRSHLKLFTIKYVDVDTNELL
                     KSEQLLTASERNLDFRDLYDPRDKAKLLYNNLDAFGIMDYTLTGKVEDNHNDTNRIIT
                     VYMGKRPEGENASYHLAYDKDRYTEEEREVYSYLRYTGTPIPDNPKDK"
     gene            complement(1485..1928)
                     /locus_tag="VHD02_001782"
     CDS             complement(1485..1928)
                     /locus_tag="VHD02_001782"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEQ5498366.1"
                     /translation="MKLVLQRVKEASVSIDGKIAGAINQGLLLLVGVGPDDNAEDLAY
                     AVRKIVNMRIFSDADGKMNQSIQDIKGSILSVSQFTLYADTKKGNRPAFTGAAKPDLA
                     SQLYDSFNEQLAEFVKVERGVFGADMQVSLINDGPVTIILDTKCH"
     gene            complement(1943..4162)
                     /locus_tag="VHD02_001783"
     CDS             complement(1943..4162)
                     /locus_tag="VHD02_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEQ5498367.1"
                     /translation="MAKIMNVTGEEVIALAATYMTKADVAFVAKALAYATAAHFYQVR
                     KSGEPYIVHPIQVAGILADLHLDAVTVACGFLHDVVEDTDITLDEIEADFGHDARDIV
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLAFRYLNETEFYKISHMMKEKRREREALV
                     EAIVSKVKTYTTQQGLFGDVYGRPKHIYSIYRKMRDKKKRFDQIFDLIAIRCVMETQS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKDMHQV
                     AEYGVAAHWAYKKGVRGKVNQAEQAVGMNWIKELVELQDASNGDAVDFVDSVKEDIFS
                     ERIYVFTPTGAVQELPKESGPIDFAYAIHTQIGEKATGAKVNGRMVPLTAKLKTGDVV
                     EIITNANSFGPSRDWVKLVKTNKARNKIRQFFKNQDKELSVNKGRDLLVSYFQEQGYV
                     ANKYLDKKRIEAILPKVSVKSEESLYAAVGFGDISPISVFNKLTEKERREEERAKAKA
                     EAEELVKGGEVKHENKDVLKVRSENGVIIQGASGLLMRIAKCCNPVPGDPIDGYITKG
                     RGIAIHRSDCHNIKSQDGYQERLIEVEWDLDNSSKDYQAEIDIYGLNRSGLLNDVLQI
                     LSNSTKSISTVNAQPTKDMKFANIHVSFGIPNLTHLTTVVEKIKAVPDVYSVKRTNG"
     gene            complement(4414..4524)
                     /locus_tag="VHD02_001784"
                     /pseudo
     CDS             complement(4414..4524)
                     /locus_tag="VHD02_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_129544913.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
BASE COUNT         1297 a         1075 c          905 g         1354 t
ORIGIN      
        1 tatctataaa attattatgt tattctaata atggggattg aaacttaact tttaggaggt
       61 ttctatgaaa aattacttat cttttgggat gtttgcactg ctgtttgcat taacatttgg
      121 aacagtcaag cctgtccaag ctattgctgg acctgaatgg ctgctagacc gtccatctgt
      181 caacaacagc caattagttg ttagcgttgc tggtactgtt gaggggacga atcaagaaat
      241 tagccttaaa ttttttgaaa ttgacctaac atcacgacct gctcatggag gaaagacaga
      301 gcaaggctta agtccaaaat caaaaccatt tgctacaaat agtagcgcaa tgccacataa
      361 acttgaaaaa gctgacttat taaaggctat tcaagaacaa ttgatcgcta acgttcacag
      421 taacgacggc tactttgagg tcattgattt tgcaagcgat gcaaccatca ctgatcgaaa
      481 cggcaaggtc tactttgctg atcgagatga ttcggtaacc ttgccgaccc aacctgtcca
      541 agaatttttg ctaagcgggc atgtgcgcgt tagaccgtat caacctaaag ccgttcacaa
      601 ctcagctgaa cgcgttaacg tcaactatga agtgagcttt gtctccgaaa caggaaattt
      661 agactttacg ccatcgttaa aagaacgata ccatttgacc acactggcag ttggtgactc
      721 tctttcatca caagagttag cagcaattgc ccaatttatc ctatcaaaag agcatccaga
      781 ttatatcatt acaaaacgtg actcctcaat cgtcactcat gacaatgaca ttttccgtac
      841 gattttacca atggatcaag agtttactta ccatatcaaa gaccgggaac aagcttataa
      901 ggccaattct aaaacaggta ttgtagaaaa aacgaacaac actgacctta tctctgagaa
      961 atattacgtc cttaaaaaag gggaggagcc gtatgatccc tttgatcgca gtcacttgaa
     1021 actgttcacc atcaaatacg ttgatgtcga taccaacgaa ttgctaaaaa gcgagcagct
     1081 cttaacagct agcgaacgta acttagactt cagagattta tacgatcctc gtgataaggc
     1141 taaactactc tacaacaatc tcgatgcttt tggtattatg gactatacct taactggaaa
     1201 agtagaggat aatcacaatg acaccaaccg tatcataacc gtttatatgg gcaagcgacc
     1261 cgaaggagag aatgctagct atcatttagc ttatgataaa gatcgttata ccgaagaaga
     1321 acgagaagtt tacagctacc tgcgttatac agggacacct atacctgata accctaaaga
     1381 caaataacca cgatcttcta aaacgatgag attaactgac aaaaaaagca agcaacacgc
     1441 tatcaacagt tgcttgcttt tttctaatct cttagttgta gagactagtg acatttcgtg
     1501 tctaaaataa tcgtaactgg tccatcattg atgagactaa cctgcatatc tgccccaaaa
     1561 acgccacgct caactttcac aaactctgcc aattgttcat taaaactatc atacaactga
     1621 ctagccaaat ctggcttagc agcaccagta aaggcaggac ggtttccctt tttggtgtca
     1681 gcataaaggg taaattgcga cacagataag atactgccct tgatgtcttg gatagactga
     1741 ttcatcttgc catcagcatc tgaaaaaatg cgcatgttga ctatttttcg cacagcgtaa
     1801 gccaaatctt ctgcattatc atctggtccc acacctacta acaacaacaa cccttgatta
     1861 atagcgccag caattttccc atcaatagaa acagaggcct ctttgacccg ttgcaagaca
     1921 agtttcatgg ttttatcctt tcttagccat tggtccgctt cacgctataa acatctggaa
     1981 ctgccttgat tttttcgaca acagtggtca gatgcgtcag atttggaatg ccaaagctca
     2041 cgtgaatatt agcaaacttc atgtccttag tcggctgagc attgactgtc gatatgctct
     2101 tggttgagtt tgataaaatt tggagcacat cattaagcag gccactacga ttgagcccat
     2161 agatatcaat ttcagcctga taatctttac tcgaattgtc caagtcccac tcgacctcaa
     2221 tcaagcgttc ttggtagcca tcttgactct taatgttatg acagtccgat ctgtgaatcg
     2281 caatgccacg ccctttggta atgtagccgt caataggatc accaggtacg ggattacaac
     2341 acttggcaat ccgcatcaag aggcctgatg ctccttggat aatgacccca ttttcactgc
     2401 gaaccttgag cacatctttg ttttcgtgtt tgacctcacc gcccttaacc aattcttcag
     2461 cttctgcttt agccttggcc ctttcttctt cacggcgctc tttttcggtt aacttgttaa
     2521 agacactgat aggactaatg tcaccaaacc caacggctgc atagagtgat tcttcgctct
     2581 tcacactgac ttttggaagg atcgcttcaa tgcgtttttt gtcaaggtat ttattggcaa
     2641 cgtagccctg ctcttgaaaa taagacacca acaaatcacg gcctttattc actgacaatt
     2701 ccttgtcttg atttttaaag aactgacgaa ttttgttgcg agccttattg gttttgacca
     2761 gttttaccca gtctcgacta gggccaaagg aattggcatt ggtgatgatt tcaaccacat
     2821 ctcctgtttt taacttggca gtgagaggaa ccatacgccc attaaccttg gcacctgttg
     2881 ctttttcacc gatttgcgta tggatcgcat aagcaaaatc aataggacct gattcttttg
     2941 gcaactcctg aacggcccct gtcggtgtaa agacataaat ccgttcagaa aaaatgtctt
     3001 ctttgaccga atccacaaag tccactgcat caccatttga ggcatcttgc aattctacca
     3061 gctctttgat ccagttcatg ccaacggctt gctcagcttg attgacctta ccacgcacgc
     3121 cttttttata agcccagtga gcagcaaccc cgtactcagc cacttgatgc atgtccttag
     3181 ttctgatttg aatctcaata ggtccttttg gtccatacac agtggtatga atagactggt
     3241 agccattagc tttaggagct gcaatataat ccttgaagcg gcctggcatg ggacgccaaa
     3301 gctcgtgaat atagccaacc atagcataga catcgctttg cgtttccatg acacaacgaa
     3361 tggcaatcag atcaaaaatc tgatcgaaac gtttcttttt gtcccgcatt ttccgataaa
     3421 tggaataaat gtgttttggt cggccataca catctccaaa caacccttgt tgtgtcgtat
     3481 aggttttgac cttactgaca atagcctcta ccaaggcttc acgctcgcga cgtttttctt
     3541 tcatcatatg ggaaattttg taaaattcgg tttcattgag gtaacgaaaa gccaaatctt
     3601 ctagttccca tttgatgcga ctaatcccca aacgatgcgc caagggggca tagatttcca
     3661 tggtttcgcg cgaaatgcgc tcttgtttgt ccttgcgcaa atgtttgagg gtgcgcatat
     3721 tatgcaggcg gtcagccaat ttcaccaaaa tcacgcgaat atctttggac atagccatca
     3781 gcattttgcg atggttttcg gcgagttgct cctcatgaga tttgtactca actttaccta
     3841 acttggtgac accatcaacg atatcacgag catcatggcc aaagtctgct tcgatctcat
     3901 ctaaggtaat gtccgtatct tctacgacat catgtaaaaa gccacaagca actgtcacag
     3961 catccagatg caaatcagcc agaatccccg ccacctgaat cggatggacg atatagggct
     4021 cgcctgactt tctcacttgg tagaaatggg ccgctgttgc atatgctaaa gcctttgcca
     4081 caaaagccac atcagccttg gtcatatagg tggccgctaa ggcaatgact tcttctcctg
     4141 ttacgttcat tatttttgcc atttcgttcc tcatctagca tttcttttcc ctattttaac
     4201 actttacaca caaaagcaaa aggcagaatc tgctaagaaa gcgcttagtg tgaccttttc
     4261 ttagaaaacg gttagtgagc tgcttttgat atcttttggg atctctcagg tgtgagcatg
     4321 ataccaaagg cctgctcccg accaaaaata gagagaacca aggggctctc tctattatgt
     4381 ttgcttatca gtaaggcctc tcaattcaga ggattagttg ttttctttgc gttttgtaac
     4441 ggctaggaca ccagctgttg tcatcactgc tactgctgct gctgtaaaga atgggttggc
     4501 ttgctcacca gttgctggta gacgtctgtg gttgttggct acaggtgtct tagccatatt
     4561 gccattgcgg cgttctggcg tttggttagc ttctttgtca gcaggttttt ctggagctgg
     4621 agttacctct t
//
LOCUS       DAUDYX010000081         4606 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.082, whole genome shotgun sequence.
ACCESSION   DAUDYX010000081 DAUDYX010000000
VERSION     DAUDYX010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.082"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(291..692)
                     /locus_tag="VHD02_001785"
                     /pseudo
     CDS             complement(291..692)
                     /locus_tag="VHD02_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558053.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1115..1648)
                     /locus_tag="VHD02_001786"
     CDS             complement(1115..1648)
                     /locus_tag="VHD02_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEQ5498368.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGVSYYCNLASPYMMDTEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEIHKKEMQYSADMDFILKENVKILVDWINHEKGPFS
                     KAYITIWYKRYLELKNR"
     gene            complement(1728..2504)
                     /gene="recX"
                     /locus_tag="VHD02_001787"
     CDS             complement(1728..2504)
                     /gene="recX"
                     /locus_tag="VHD02_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEQ5498369.1"
                     /translation="MKITKIEKKKRLYLIELDNDESLYVTEDTIVRFMLSKDKVLDND
                     QLEDMKHFAQLSYGKNLALYFLSFQQRSNKQVADYLRKHEIEEHIIPDIITQLQEEQW
                     IDDTKLADTYIRQNQLNGDKGPQVLKQKLLQKGIASHDIDPILSQTDFSQLAQKVSQK
                     LFDKYQEKLPPKALKDKITQALLTKGFSYDLAKHSLNHLNFDQDNQEIENLLDKELDK
                     QYRKLSRKYDGYTLKQKLYQALYRKGYNSDDINCKLRNYL"
     gene            2619..3974
                     /gene="rlmD"
                     /locus_tag="VHD02_001788"
     CDS             2619..3974
                     /gene="rlmD"
                     /locus_tag="VHD02_001788"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEQ5498370.1"
                     /translation="MVVKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIFC
                     QITSVKRNFAEAKLLMVNKASKNRVKPACSVYETCGGCQIMHLAYPKQLDFKDDVIRQ
                     ALKKFKPTGYEQFEIRPTLGMKKPDHYRAKLQFQLRSFGGTVKAGLFSQGSHRLVPID
                     NCLVQDQLTQDIINKITQLVDKYKLPIYNERKIAGIRTIMVRKAQASDQVQIIVVSSK
                     EVRLARFIGELTKAFPQVKTVALNSNRSKSSEIYGDETEILWGQEAIHEEVLDYGFAL
                     SPRAFYQLNPQQTEVLYGEVVKALDVGSKDHIIDAYCGVGSIGFAFAGKVKSVRGMDI
                     IPEAIEDAQKNAKAMGFDNAYYEAGKAEDIIPKWYKQGYRADAIIVDPPRTGLDDKLL
                     KTILHYQPKQMVYVSCNTSTLARDLVQLTKVYDVHYIQSVDMFPHTARTEAVVKLQKR
                     V"
BASE COUNT         1403 a          720 c          957 g         1526 t
ORIGIN      
        1 ctatttagta actttagtta aagtaatcga actaacttag ttgattgtat tttaatgata
       61 aaaagcttaa atttgttgta taacaaattt aagcttttta ttgtttcttt ttaattacag
      121 attgaggttt aaaagacact ttacaatgtt ctcaagtcat gtgttgttgt aggttgtatg
      181 cacatattac aaatcccatt ataaaagaaa ttgtttaatt aatggatagt aggatggtat
      241 ggagatctta atgtggtttt aatggtaatt ttagcagtta tagtaagaca aaaagtgact
      301 tggaaaagct tatacagacg attcttaact cccacaatat cttctgtcag attttattag
      361 acttcttctt ggacatgagt tggtttacgt tttagtacaa attgagattg agctagtgtt
      421 tcagtgtcaa ttttgtatga tttacgaacg tgcaggctat tcaattgctt cttagcttct
      481 aaaggattaa accgcattat ctgcgaaaaa acctgaagac gacgagaata gacattaatc
      541 gctttaagga taatctcagg gttatggacg gttttcaagt ttccaagaag acgattgagg
      601 ccgatggtat cgttttgtct tgaatgattc cttattttag tggtttcatt ttcaatacac
      661 gacgtcaagc gtcctacata ctttgataaa attctaccta aaagaggtgt cttgtcagtt
      721 ttgattacaa catcaagtgc caaaagactt tgcgacttaa caagacactt ctactttaac
      781 gcaaagaaaa agtagtgagt actctcttcc gtcggagatt tcctcgctac taaacttagt
      841 attttttgat actttcctta ggtagtgctt aaggcagcga cttcctttgg aattccatgc
      901 cttatttttg agatcctagg ctcaaaaata ccaagtggcg tcaatgttag tgttgacgcc
      961 acttttacca caacgaaaaa catctgtttt ggctcactcc acacgcagaa ataggaaatt
     1021 ggatgtcaaa tcagcagaga gctgttcatg ctttttgagc ctaaaactga gtttgtctgc
     1081 gttctgagca ctccttggga gtgctggaca actcttaacg attcttcagt tcaagataac
     1141 gtttgtacca aatagtgata taggccttgg aaaatggtcc tttttcatga ttaatccaat
     1201 caaccaatat tttgacattt tcttttagga taaagtccat atcggctgaa tactgcatct
     1261 cttttttatg aatctcgtat tcatcaacat caagtaatct cttttcacca tccgcaaaga
     1321 ctttgacatc taaatcgtaa tcaatgtatt tgagggcctc agtgtccatc atatatggac
     1381 tagctaagtt acagtaataa gagacgccat tatctcgaat catggctata atgttaaacc
     1441 agtatttttt atgaaagtaa acgattgcag gttcgcgggt cacccatcgt ctaccgtcac
     1501 tttctgtgac aagagtgtga tcattaactc caatgagggc attttcagtt gtttttagta
     1561 ccatagtatc gcgccaagta cggtgcaaac taccatcatg cttataactt tgaattgtaa
     1621 taaagtcgcc ttctttaggt aatttcatgc cttaccaact ttctacaatt aaacttatcc
     1681 tctatagtct atcataaatg tgtgaaaaaa tcacttcttt tgtcagtcta taaataattt
     1741 cttaacttgc aattaatatc gtcgctgttg tagccttttc ggtagagagc ctgatagagc
     1801 ttttgcttta aagtataacc atcatatttg cgactgagtt tacgatattg tttgtctaat
     1861 tctttgtcaa gaagattttc tatttcttga ttatcttggt caaaattaag gtgattgagg
     1921 ctatgtttag ctagatcgta tgaaaagcct ttggtcagta atgcttgggt gattttatct
     1981 ttcaaggctt ttggtggcaa tttttcttga tatttgtcaa agagtttttg gcttactttt
     2041 tgagcgagtt ggctaaagtc agtttgagat aagataggat caatgtcatg acttgcaatg
     2101 cctttttgta ataatttttg ttttaatact tggggacctt tatcaccatt taactgattt
     2161 tggcgaatgt aggtatcagc caatttggtg tcgtctatcc attgttcttc ttggagttga
     2221 gtgatgatgt caggaataat gtgttcttca atctcatgct tgcgcaggta atcagcaact
     2281 tgcttgttgc tgcgttgttg aaaggaaaga aaataaaggg ctaaattttt gccgtaggac
     2341 agttgggcaa aatgtttcat gtcttcaagc tgatcattgt caaggacttt atctttactc
     2401 aacataaacc gaacaatagt atcttctgtt acataaaggg attcgtcatt atccaattcg
     2461 ataaggtaga ggcgtttttt cttttcaatt ttagtgattt tcatactttg attatatcaa
     2521 aaatggtaaa atagaagacg aaaatactgc taaaaatgtg ttgcttgtga atgcttttgg
     2581 tggtgagatt aggatattag aggaagtaag gggaagagat ggtcgtaaag gttaaacaga
     2641 aaattccttt aaaaattaag cgaatgggaa ttaacggtga ggggattggt ttttatcaga
     2701 aaactttggt atttgtccct ggtgctttaa agggagaaga tattttttgc caaatcacgt
     2761 cggttaaacg gaattttgca gaggccaaac tgttgatggt aaacaaggct tctaagaatc
     2821 gtgtcaaacc agcttgttct gtttatgaaa cttgtggtgg ttgccaaatc atgcatctag
     2881 cctatcctaa acagctagat ttcaaagatg atgtgattag gcaggccctt aaaaagttca
     2941 aaccaactgg ctatgaacaa tttgagatta gaccgacttt aggaatgaaa aagccagatc
     3001 actatcgagc caaattgcag tttcagctgc ggtcatttgg cgggacggtc aaggctggtt
     3061 tattttccca gggaagtcat cgcttggttc ctattgacaa ttgcttggta caggatcaac
     3121 taacgcaaga tattatcaat aagattacgc agttagtgga caagtacaag ttgcccattt
     3181 acaatgagcg aaagattgct gggattcgga cgataatggt tcgtaaggca caggctagtg
     3241 atcaggtaca gattattgtt gtgagtagca aagaagttag gttagcgagg ttcattggcg
     3301 aattgactaa ggcatttcct caggttaaaa cggttgcctt gaatagtaat cgttctaaat
     3361 cgagtgagat ttatggggac gaaacggaaa ttctatgggg acaagaggcg attcatgaag
     3421 aagttttaga ctatggattt gcgttatcac cgagagcttt ttaccaactt aatccacagc
     3481 agacagaagt tttgtatggt gaggtggtga aggccttgga tgtagggagc aaagatcata
     3541 ttattgatgc ctattgtggg gttggctcga ttggctttgc ttttgcaggg aaggtcaagt
     3601 ctgtgcgggg gatggatatt atcccagaag cgattgagga tgcacaaaaa aatgccaaag
     3661 ccatgggctt tgataacgct tactatgagg cagggaaggc agaggatatt attcctaaat
     3721 ggtataaaca aggttaccgc gcggatgcca tcattgtgga cccaccaaga actggtttag
     3781 atgataaatt attaaaaacg attcttcatt accagcctaa acagatggtt tatgtgtctt
     3841 gtaatacctc cactttggcc cgcgatttgg tgcagctaac aaaggtttat gatgttcact
     3901 atatccaaag tgtagacatg ttcccccata cagctaggac cgaggctgtc gtgaagttac
     3961 aaaaacgtgt atgattgagt ggcaataaca actcaagttt tataagctca ggtaatgggt
     4021 ttgagcaata gcttgacata gtagggatgt caaaaacggt ttactaagca tagtcatatt
     4081 attgataact ctatgtttat tttagaaaca ttcatggtta aaaaagtttg ttagtttgtc
     4141 actaagtagg gtctatttga agagcaagta cacacagaca atattagggg aattgattgt
     4201 taaaaaaagc tgttttttgc tataaattag aaaactgagt ggtcaggagg ttacattacc
     4261 agaagttatt ttagtatgct aatatgaacg atgatataga ggatttatcc tgatttaatc
     4321 ttttaagctg taagaaacct ttgcaaaaac tgtaggctgt gtgagtcttt atgtgttaga
     4381 tattaaagat aaatgcaaca cttttttata atgagatcaa caactgataa atctgagttg
     4441 ataaccagta aaatgatcgg aatgtagaaa agttacttaa tcaagctttt cgtaatttaa
     4501 ctgttagaat gacagaactt atgataaaat aaagaatatg attaaaatga aaaagctcag
     4561 tttatctatt acggagttcg aaatgaaaag aaaatcgaga tgtgat
//
LOCUS       DAUDYX010000082         4465 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.083, whole genome shotgun sequence.
ACCESSION   DAUDYX010000082 DAUDYX010000000
VERSION     DAUDYX010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.083"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(<1..472)
                     /locus_tag="VHD02_001789"
     CDS             complement(<1..472)
                     /locus_tag="VHD02_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronidase"
                     /protein_id="HEQ5498371.1"
                     /translation="MSRDPTLLIDESNLTIGSDGRAYYTFTADGDTKSVKIANNKCIG
                     TTRFNQLMIERGGKPTNYVAPVVVEGTGNPTGLFKDLKEISLELTDTKNSKLWSKIKL
                     NNQGMIEEYYNGTIKSEIIKTAEGTQQRISSETDKKLALINETVSGIRREYQDAD"
     gene            complement(483..812)
                     /locus_tag="VHD02_001790"
     CDS             complement(483..812)
                     /locus_tag="VHD02_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498372.1"
                     /translation="MRGHNLLKDLNNIIINNKTIDRIICNNTIVYQRSWNLLFDGLLT
                     SAGKKLDIYPFYKIVINNAFFVKTPTTKNNTIFLAGHKILALQDSHFCFFRALEKSFF
                     VKIYGKN"
     gene            complement(799..2022)
                     /locus_tag="VHD02_001791"
     CDS             complement(799..2022)
                     /locus_tag="VHD02_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498373.1"
                     /translation="MTLEERIPIKVLFDRKDAAEWQKLNPVVDDGELVVELDTHRLKV
                     GDGKLTYNDLPYYEGPQGESITKVQLSENGDLSVWIGEKETKLGNIKGQKGDKGTSIT
                     DITKDGETLTIKLSDDTQKTFNIPNGQKGDRGKSVESARVDESGHLKLKIEEESELDL
                     GNVKGESGPKGDSITITGQQRVSEGVQLTFSDKTKVVIPKGEKGDTGDVNGINLEDYV
                     KKSELKNVGSADVKAINDFLGLSQKVFTSSYSYTDSLLKSYAKPSYSASWYVNESTVS
                     TKNGDKVLITIHNTTTQADNYLEVVVTYVGANYVTATSTGRLLTTPGEVKVVTKKQAK
                     EDYAAKKHKHEISDIAGLNERLSGYLRQADIQSQLNNIGKLKDTQTGQYLEVKVVDKG
                     QVPSNTSGMIVFERS"
     gene            complement(2019..4166)
                     /locus_tag="VHD02_001792"
     CDS             complement(2019..4166)
                     /locus_tag="VHD02_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ5498374.1"
                     /translation="MIPVLYEAKETKFRTFGLGEIADAYEVKATRERNGNYSLYIKYP
                     LDGVFASVFKEEMKIKSDAGRRTKWQTFEINRVLRNSKDHIEIFARHISMRTQDIALK
                     PFVNGASVGAESALEIWKENLVGDDTFDVKSDILTLGSFDWEVDKIGNARGALGGVAG
                     SILDVYGGEYEFDNRTIILHKQMGRKAPTVLEYGRNIVSVEEERLLDGNYTSIYPYVR
                     YTPQPKPQEETPGKPHVGEHKQPEEQLVTLPEFILDGQYLSLYAQRRIQMVDLSSHFN
                     DDKNKKEPTIEEIRKLAQKYLKDNNVGAPKVSIEVDYIDLSQTLDYQDFRVMEEVELC
                     DIVPLYYPKFGITTESEKVVEIVYDVYTDSNHTIKLGTIGQSISKSLTGGVSERINAL
                     ENNQKVITNNQKQFELNLPKYLNDINGNRVWYEKPDDNIEHKIGDYWFEKNGKYQRTW
                     IWDGNRWVKVLDTEDLNPNQRAFDEAMAEIEKAKKAQEEINQRTDKELEEFRATLKNL
                     ALPEEAIKKITEAIKVDDIPSIKQSFDDLKNKVSETSETSRLNAEILGNNGKTRYNKN
                     LLVGDPNRVKKIDEDYIEVEANDGGFKRGETYTISFSQTCELLKKVAITLTQINNKGV
                     KLVLTPTKAKMEAQTFEVAKDKQSIEVYPLSYTAVLTGDWYKSKQIDLNASEVQELAL
                     DMSYKEIADAKGATIIGKQSDKPKIILDGRRDR"
     gene            complement(4163..>4465)
                     /locus_tag="VHD02_001793"
     CDS             complement(4163..>4465)
                     /locus_tag="VHD02_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ5498375.1"
                     /translation="DNGGTLQNPGNVQAKPILRIKGTGDGVLTINGFETGLENVQREL
                     VIDMERHLVYKDVLSAWDNIVRTERHRMPLFDVGQNKISWTGSFTITAVPNWGVKV"
BASE COUNT         1130 a         1000 c          809 g         1526 t
ORIGIN      
        1 tatctgcatc ttggtactca cgtctaatgc ctgagactgt ctcgttgata agcgcaagtt
       61 ttttatcggt ctcgctacta atacgttgct gcgtgccctc tgcggttttg ataatctcag
      121 attttattgt accgttgtaa tactcttcaa tcatcccttg attattaagc ttgatttttg
      181 accaaagttt ggagttttta gtatctgtta actcgaggct aatctctttg aggtctttaa
      241 atagtccagt cggattacct gtcccctcga caaccacggg cgccacgtag ttagttggtt
      301 taccccctcg ctcaatcatg agctggttaa agcgagttgt accgatacat ttgttattgg
      361 ctattttaac gctttttgtg tcaccatcag ccgtaaatgt ataataagca cgtccatctg
      421 agccgattgt taaatttgac tcgtctatta aaagtgttgg gtctctactc aaatgttacc
      481 tcctaatttt taccgtaaat tttgacaaaa aaactttttt ctaatgctct aaaaaaacaa
      541 aaatgactat cttgtaatgc tagtatttta tgccctgcta aaaaaatcgt attgtttttt
      601 gttgtggggg ttttaacaaa aaatgcatta ttgattacga ttttataaaa aggataaata
      661 tccaattttt tcccagcgct tgtcagcaaa ccgtcaaaaa gcagattcca cgaccgctga
      721 tagacaatcg tattattaca aataatacga tcaatcgttt tgttattaat aatgatatta
      781 tttaaatctt ttaacaaatt atgacctctc aaacacgatc atgccactgg tattgctagg
      841 cacctgtcct ttatcaacca ctttaacctc aagatattgt ccggtttgcg tgtcttttag
      901 cttaccgata ttattaagct gtgactgtat atcagcttgt ctgagatagc cagacaagcg
      961 ctcattaaga ccagctatgt cactaatctc atgcttatgt tttttagcag cataatcttc
     1021 ttttgcttgc ttttttgtca ctactttgac ttcaccagga gtagtcagta agcgacctgt
     1081 ggaagtagca gtcacgtagt tagcaccaac ataagtcacc actacctcca aataattgtc
     1141 tgcttgggtg gtagtgttat gtattgtaat caatactttg tcaccatttt tagtagaaac
     1201 tgtagattcg ttgacatacc aactcgccga atagctgggt tttgcataac tttttagtaa
     1261 gctatctgta taactatagc tgcttgtaaa caccttttga gatagcccta aaaagtcatt
     1321 aatagcttta acatctgcgg aaccgacgtt tttaagttca gattttttga cataatcttc
     1381 cagattgatg ccgttgacat caccagtgtc gcctttttct cctttcggaa taacgacctt
     1441 ggttttatca ctaaaggtca gctgtacgcc ctctgagaca cgttgttgac ctgtgatagt
     1501 gatactatca cctttaggtc ctgactcacc tttaacgttt ccaagatcta actctgattc
     1561 ctcttcaatt tttagtttta aatgaccgga ttcatcaacc cgagcactct caacgctctt
     1621 acctctatcc cctttttggc cattggggat attaaaggtt ttttgagtgt cgtctgatag
     1681 tttgatagtg agcgtctcac catctttggt gatgtcagta atacttgtac ctttgtcacc
     1741 cttttgacct ttgatatttc cgagcttagt ctctttttcc ccaatccata ccgataagtc
     1801 accattttcg gatagctgta ccttagtgat agattctcct tgcggacctt cgtagtaagg
     1861 caggtcattg taagtcaatt tgccatcacc gacttttagc ctgtgagtgt ctagctcaac
     1921 aactagttca ccatcatcaa caacaggatt taatttttgc cactcggcag catccttgcg
     1981 gtcaaataag actttaattg gtattctttc ttctagcgtc atctgtccct ccttccgtct
     2041 aaaataattt ttggtttatc ggactgtttg ccgataatag ttgcaccttt ggcatctgca
     2101 atctctttat agctcatatc cagggccaat tcctgcacct ccgacgcgtt taaatctatc
     2161 tgcttagatt tataccagtc gccagttaaa acagccgtgt agctcaaagg ataaacctct
     2221 atagactgtt tatccttagc gacctcaaac gtctgtgctt ccatcttagc cttggtaggt
     2281 gtcagcacta acttaactcc cttgttgtta atttgcgtca atgtgatagc cacttttttg
     2341 agtagctcac aagtctggct aaagctaatc gtgtaggtct cgccacgctt aaaaccaccg
     2401 tcgttagctt ctacttctat gtaatcctca tcaatttttt taacacgatt aggatcgcca
     2461 accaagaggt ttttgttgta gcgggtctta ccgttattcc ctaaaatttc ggcgtttaga
     2521 cgggatgtct cgctcgtttc actgaccttg tttttgaggt catcaaagct ttgtttaatt
     2581 gatgggatgt catcaacttt gatagcctct gtgatttttt taatcgcttc ctctggtaac
     2641 gctaggtttt tgagggtggc tcggaattcc tctagctctt tgtcagtacg ctggttaatt
     2701 tcttcctgcg cttttttagc tttttcgatt tccgccatcg cctcatcaaa agctcgttga
     2761 ttggggttta aatcctctgt atctagtact ttaacccatc gattgccatc ccaaatccat
     2821 gtgcgctgat acttcccatt tttttcaaac cagtaatcac ctatcttgtg ctcaatattg
     2881 tcatctggtt tttcgtacca gacacggtta ccattgatgt cattgaggta tttaggcaga
     2941 ttgagttcaa actgtttttg gttgttagta ataacctttt gattattttc caacgcattg
     3001 atacgttcgg aaacaccacc agtcaaactt ttagagatgg actgaccaat cgtgccaagt
     3061 tttattgtat ggttgctatc tgtatagacg tcatagacaa tctcaacaac tttttcagac
     3121 tcagttgtga tgccaaactt tggataataa agtggtacaa tgtcgcaaag ctcaacctct
     3181 tccatgactc taaaatcttg atagtcaagc gtttgtgaca agtcaatata atcaacctcg
     3241 atactgactt ttggtgcgcc tacgttatta tccttaaggt atttctgagc cagctttcgg
     3301 atttcttcga tcgttggctc ttttttattt ttgtcatcgt taaaatgact tgataaatca
     3361 accatttgga ttctgcgctg agcatataag ctgagatact gaccatctag gataaattca
     3421 ggcaatgtca ctagctgttc ttcgggttgt ttatgctcgc ctacatgcgg cttaccaggg
     3481 gtttcctctt gcggttttgg ttgtggcgta tatcttacgt aaggatagat agaggtgtaa
     3541 ttgccatcta gcaatcgctc ctcctctacg ctgacgatat tacggccata ctcaagcacc
     3601 gtaggagctt tacgacccat ctgcttatgt aagatgatgg tgcgattgtc aaattcatac
     3661 tcaccaccgt agacatctag gatagagcca gcgacacctc cgagagcgcc acgggcatta
     3721 ccgattttat caacttccca gtcaaaactc ccgagcgtta ggatgtcgct tttaacgtca
     3781 aaagtatcat caccaacaag gttttctttc cagatttcta aagctgattc ggctcctacg
     3841 ctcgcaccgt ttacaaacgg ttttaaagcg atatcctgtg tgcgcataga aatatgacgc
     3901 gcaaaaatct caatgtggtc tttactattt cgtagtaccc gattaatctc aaaagtctgc
     3961 catttggttc tacgaccagc gtcagactta atcttcattt cctctttaaa aactgaggca
     4021 aagacaccat ctagcggata tttgatgtac agtgagtaat taccattgcg ctcacgagtg
     4081 gctttaacct cataagcatc cgcaatctca ccgagaccaa aagtcctaaa cttggtttcc
     4141 ttagcctcat acaaaactgg tatcatactt taacccccca gtttggcact gcggtaattg
     4201 taaagctacc agtccacgag attttatttt gtccaacgtc aaataacggc atgcggtggc
     4261 gttctgttct tacgatatta tcccaagcag ataggacgtc tttatagaca aggtgccttt
     4321 ccatgtctat gacgagttcc ctttgcacat tttcaagtcc tgtctcaaaa ccgttaatgg
     4381 ttaaaacacc atcgcctgtg cctttgattc ttaagatagg tttagcctgt acattgccgg
     4441 gattttgtag agtaccacca ttatc
//
LOCUS       DAUDYX010000083         4333 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.084, whole genome shotgun sequence.
ACCESSION   DAUDYX010000083 DAUDYX010000000
VERSION     DAUDYX010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.084"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..218
                     /locus_tag="VHD02_001794"
     CDS             <1..218
                     /locus_tag="VHD02_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEQ5498376.1"
                     /translation="EMSQKIMAKIQDWNRMPILKLIEWKYFLIKQKDIIKAEQSFMKA
                     CLFAQMTADQYLENKLIQEWEKDVKSY"
     gene            302..397
                     /locus_tag="VHD02_001795"
     CDS             302..397
                     /locus_tag="VHD02_001795"
                     /inference="COORDINATES: protein motif:HMM:NF038244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system DWW-type pheromone"
                     /protein_id="HEQ5498377.1"
                     /translation="MLKKYKYYFIFAALLSFKVVQELSAVDWWRL"
     gene            444..1442
                     /gene="ruvB"
                     /locus_tag="VHD02_001796"
     CDS             444..1442
                     /gene="ruvB"
                     /locus_tag="VHD02_001796"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023079556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEQ5498378.1"
                     /translation="MARILDNDVMGNEEFSDRTLRPQYLREYIGQDKVKEQFAIFIEA
                     AKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAVEKAGDLVAILNELE
                     PGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPR
                     IANRLLKRVRDYAQIIGDGIITAQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGG
                     PVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQKAYRHLGYPYQNT"
     gene            1580..2017
                     /locus_tag="VHD02_001797"
     CDS             1580..2017
                     /locus_tag="VHD02_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEQ5498379.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMKSIVSSDVMMIESRATSDWEHG
                     KPIHSGTQSILKTYQINYDITKCSKQITITDFNTFDYIIGMDSDNVKNLKEMSQHQWD
                     SKIYLFREGGVPDPWYTNDFEETYQLVRKGCQDWLSRLMNKEY"
     gene            2041..2442
                     /locus_tag="VHD02_001798"
     CDS             2041..2442
                     /locus_tag="VHD02_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498380.1"
                     /translation="MINMQDIVKKWSITRAKLEIVSVIVILVCAISVFSVRISNKTSL
                     TYDKGRMHYTGYVINHKMNGEGKLVYPNGDIYEGTFKNGLFEGKGTFTAKTGWLYNGE
                     FHKGQANGKGVLKAKNNKVYKGIFKQGIFQK"
     gene            2439..4214
                     /locus_tag="VHD02_001799"
     CDS             2439..4214
                     /locus_tag="VHD02_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEQ5498381.1"
                     /translation="MRIKWFSFVRVTGLLLVLLYHFFKNVFPGGFIGVDIFFTFSGYL
                     ITALLIDEYTKKESIDIIGFLKRRFYRIVPPLVLMILLTIPFTFLIKKDFIANIGSQI
                     TAVLGFTTNIYEILTGSSYESQFIPHLFVHTWSLAIEVHFYLFWGVFVWLLARRKETQ
                     KQLRGLLFLISLGIFAISFLSMFIRSFMTSNFSLIYFSSLSHSFPFFLGAMFATITGI
                     NETTVRFQKNVRLWPRQYVLAAMVGAFTLLLVLTVTLDFNHITTYLFGFALASLFASI
                     MIYAARVLHEQTPDVQEPKAITYIADISYGIYLFHWPFYIIFSQLMSHILAVILTVFF
                     SILFATVSYYIVEPLVQGRKPNLLGLEIDCSPYYKWIVGGTLALALLTLGTCMIAPKV
                     GKFEKQLLVSSLQQAQSNMERTHTLAAGDANALSDVGIIGDSVALRSSAAFSKIMPQA
                     QLDAAVSRNFKEAFDLFNNQIKSKSLSKTVVLAVGVNSLDNYSQAVQSFIEALPNGHR
                     LVLVSPYNAKNASQVAEARDYGLKLSKKYKYVTIADWYKVAVEHPDIWYGSDGVHYSE
                     DSQGAELYVSTIQTAVEKSAKKPAK"
BASE COUNT         1368 a          656 c          875 g         1434 t
ORIGIN      
        1 ttgaaatgag tcaaaaaatt atggcgaaaa ttcaagattg gaatagaatg cctattttaa
       61 aactaataga atggaaatat tttttaatca aacaaaaaga tataataaaa gcagaacaat
      121 catttatgaa ggcatgtttg ttcgcccaaa tgacagctga tcagtatttg gaaaacaagt
      181 taattcaaga gtgggaaaaa gatgttaaaa gttattaaca ggacaaaaat gtcattttta
      241 aatatttaat agtaagatat aattcctaat ataaaaaata attattaagg agtttttatc
      301 catgttaaaa aagtataagt actattttat attcgcagct ctactatctt ttaaagtagt
      361 tcaagagctt agtgctgttg actggtggcg attataataa aagtaggcca ctgctctctt
      421 ttactttatg ttataatagt cctatggcta gaattttaga caatgatgta atgggaaatg
      481 aggagttttc ggatcggact cttcgccctc aatatctacg cgaatatatt ggacaagata
      541 aagttaagga acaattcgct atttttatag aagcagctaa aagacgtgac gaatctttag
      601 accatgtttt attatttgga cctccaggac tgggaaaaac aacaatggca tttgtgattg
      661 ccaatgaatt gggagttaat ttaaagcaaa cttcaggtcc tgccgttgag aaagctggag
      721 atttggtagc tattttaaat gaattagaac caggggatat tttgtttatt gatgaaattc
      781 accgcatgcc aatgtctgtt gaggaagtac tatatagtgc tatggaagac ttctatattg
      841 atattatgat tggcgctgga gatactagtc gtagtattca tttggatctg cctccattta
      901 cattgattgg tgctactaca cgtgcaggca tgttatctaa tcccctcaga gctcgttttg
      961 gtatcactgg tcacatggaa tactatcaag aaaaggattt gacagagatt gtggagcgta
     1021 cagctactat ttttgaaata aaaatagacc atgaagctgc acggaaatta gcttgtcgca
     1081 gtagaggaac gccccgcatt gctaatcgat tactaaaacg agtgcgtgac tatgctcaaa
     1141 ttattggtga tggcatcata acggctcaaa taactgatag agctttgact atgttagatg
     1201 ttgaccgcga aggtctcgat tacattgatc agaaaatttt acgaacgatg attgagatgt
     1261 atcagggagg accagttggt ttggggacat tatctgtgaa tattgccgag gagcgtaata
     1321 cagttgaaga gatgtatgaa ccctacttaa ttcaaaaagg ctttctcatg cgaacacgaa
     1381 caggtcgtgt agcgactcaa aaggcttatc gtcatcttgg ctatccctat caaaacactt
     1441 aatgattttt caaatctaag aaaattaatc tgaacctgct ttttgtgttc ggattttttg
     1501 ataagttgac agaaaattat tagattgatt tctccgatat cagttagaaa tttgatataa
     1561 taattcaaag gagtatttaa tgaaaaaagt atgttttgtc tgtctgggta atatttgtcg
     1621 aagccccatg gctgagtttg taatgaagtc aatagtatca agtgatgtta tgatgataga
     1681 aagtcgtgct acttctgatt gggagcatgg aaaacctatt cactcaggta cacagtccat
     1741 attaaaaact taccaaatta attatgatat aactaaatgc tccaagcaga taactataac
     1801 tgattttaac acctttgact atattattgg tatggatagc gataatgtga aaaatctcaa
     1861 agagatgtcg caacatcaat gggactctaa aatctatctg tttagagaag ggggtgttcc
     1921 tgatccttgg tacactaacg attttgaaga aacctaccaa ttagttagaa aaggatgcca
     1981 ggattggctt tctcgtttaa tgaataaaga gtattgataa ttagaagttg agtaaaagaa
     2041 atgataaata tgcaagatat agtaaaaaaa tggagtatta cacgggcgaa gttggaaatt
     2101 gtctcggtga ttgtcatatt ggtttgtgca atttctgttt ttagcgtaag aatttcgaat
     2161 aagacaagtt tgacctatga taaagggcgt atgcactata ctggttacgt gattaaccat
     2221 aaaatgaatg gagaagggaa gcttgtctac cccaacggtg atatctatga gggtactttt
     2281 aagaatggcc tatttgaagg aaaaggaact tttactgcta agacgggctg gttatataac
     2341 ggggaatttc ataaaggaca agcaaatggc aaaggtgtgt taaaagcaaa gaataataaa
     2401 gtctataaag ggatttttaa acaggggata tttcaaaaat gagaataaaa tggttttcgt
     2461 ttgttagggt aacaggactt ttactggttt tactctacca cttttttaaa aatgtatttc
     2521 caggaggttt tattggtgtt gacattttct tcaccttttc aggatatcta atcacagccc
     2581 tcttgattga tgaatacaca aaaaaagagt ctatcgatat tatcggtttt ttaaaaagac
     2641 gcttttatcg aattgttcct cctttggtgt taatgatttt attgaccatt ccttttacgt
     2701 ttttaattaa gaaggatttc attgctaata taggaagtca gattacagct gttttaggat
     2761 ttaccactaa tatttatgaa atattgacgg gaagtagtta tgaaagccaa tttattccac
     2821 atctctttgt tcatacgtgg agcttagcta ttgaagttca tttttacttg ttttggggtg
     2881 tatttgtttg gttattagct agacgcaagg agactcagaa acaactgaga ggactactgt
     2941 ttttgatttc tttgggaata tttgctatta gtttcttgag catgtttata agatctttta
     3001 tgacctcaaa tttttccctg atttattttt cgagtctttc tcacagtttt cccttctttt
     3061 taggggcgat gtttgccacg ataacaggta ttaacgagac tactgttcga tttcaaaaaa
     3121 atgtacgttt atggccgcgc cagtatgtcc ttgctgctat ggttggagca tttacacttt
     3181 tgcttgtgct aacggtcaca ttggatttta accatatcac gacctatcta tttggctttg
     3241 ctttggctag tttattcgca tcaattatga tttatgcagc gcgtgttctt catgagcaaa
     3301 caccagatgt acaagagcct aaagctatca cttatatagc tgatattagc tatgggattt
     3361 acctcttcca ttggccattt tacattattt ttagccaatt aatgtctcat attttggcag
     3421 ttattctaac agttttcttt tcaatcttgt ttgccactgt gtcttactat attgtagaac
     3481 ctttggtcca agggagaaaa ccaaacctgc ttggtttaga aattgactgt tctccttact
     3541 ataagtggat tgtaggaggg actctcgcct tagcattatt aactttagga acttgtatga
     3601 tagcgcctaa agttggtaag tttgagaaac aattgttggt tagttcccta caacaggctc
     3661 aaagtaatat ggagcggaca catacactag ctgcaggaga cgctaatgct ttgagtgacg
     3721 taggtattat tggtgattct gtagcattac gctctagtgc agcattctca aaaattatgc
     3781 cacaagcgca actggatgca gcggtcagtc ggaattttaa agaagctttc gatttattta
     3841 ataaccaaat taaatctaag agtctatcta agactgttgt cttagcagta ggtgttaact
     3901 cgcttgacaa ctattctcaa gcagttcagt cttttattga ggcattgcca aatggtcatc
     3961 gtttagtttt ggtttctcca tataatgcca aaaatgccag tcaggttgca gaagctagag
     4021 actatggtct taaattgtcc aaaaaataca agtatgtcac tattgcagac tggtataaag
     4081 ttgctgttga acatccagat atttggtatg gcagtgacgg tgttcattat agtgaagata
     4141 gtcaaggagc agaactatat gtctccacaa tccagactgc tgttgagaaa tcagctaaaa
     4201 aaccagctaa ataaagttaa gaaatgtcta agagataagt cagatccatt gtgctctggc
     4261 tttttcttgt gaacaaatga tgattgtaaa atagtagtac tttgaggttt tttaaaagaa
     4321 agcggtttca gaa
//
LOCUS       DAUDYX010000084         4081 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.085, whole genome shotgun sequence.
ACCESSION   DAUDYX010000084 DAUDYX010000000
VERSION     DAUDYX010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.085"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            191..397
                     /locus_tag="VHD02_001800"
     CDS             191..397
                     /locus_tag="VHD02_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498382.1"
                     /translation="MEIKDMKVSVDVRIKLSNSEAFREMVREFNTKAHELSLIAHELE
                     MFVFEGEIENRVLSDDCNEQSDLA"
     gene            416..757
                     /locus_tag="VHD02_001801"
     CDS             416..757
                     /locus_tag="VHD02_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498383.1"
                     /translation="MRPKRYPYQKNKLIIQSKNAKTKIELDSGKITINAEEIKVGDLA
                     NEISKVQLANKEIAETIGQDKKSTETVDSRISQLEKEVEGLKGVVGTAYRLATETYHR
                     ISKQSSTSVTE"
     gene            complement(715..936)
                     /locus_tag="VHD02_001802"
     CDS             complement(715..936)
                     /locus_tag="VHD02_001802"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498384.1"
                     /translation="MSKLTPKNEIQKVYKDWDTIDTVLKYVFEDPIRTDYVAYIYPKE
                     FKIPKDEIIQELQNNGYSVTEVEDCLEIR"
     gene            1080..1265
                     /locus_tag="VHD02_001803"
     CDS             1080..1265
                     /locus_tag="VHD02_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ5498385.1"
                     /translation="MWEKIEQLMIERNLNMNKLAKLTGIHKSHFSDLKSGRIKHLSWP
                     NMVKLSTGLKVSLDEFK"
     gene            1343..1600
                     /locus_tag="VHD02_001804"
     CDS             1343..1600
                     /locus_tag="VHD02_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498386.1"
                     /translation="MQSQLTYDLLKKQIAEELFDEFKELIQEKDLANRWVNQTALVNE
                     YGYSWQTIKRMESYGLKSFKNGKDKMYCLADVNEIKHLMKQ"
     gene            1630..1770
                     /locus_tag="VHD02_001805"
     CDS             1630..1770
                     /locus_tag="VHD02_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498387.1"
                     /translation="MKLLDFIFAKPKKQEEKPKWTIENNGWEANARRYNQKHGLPAKQ
                     IW"
     gene            1898..2077
                     /locus_tag="VHD02_001806"
     CDS             1898..2077
                     /locus_tag="VHD02_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498388.1"
                     /translation="MTTTKKLGRPTSNPKSHSKRLRMTDEEVQKLEYCTKQTGKTQTD
                     VLMLGLDKVYQELKK"
     gene            2250..2522
                     /locus_tag="VHD02_001807"
     CDS             2250..2522
                     /locus_tag="VHD02_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498389.1"
                     /translation="MDNSKLENHQKVTVVCTDFNVYLNGIRLTGARPETAKLAERCGE
                     KLITLSLVVTDFDDHRTPKMADMAKEAEMANKTLDWEKKHFYKSNK"
     gene            complement(2512..2892)
                     /locus_tag="VHD02_001808"
     CDS             complement(2512..2892)
                     /locus_tag="VHD02_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498390.1"
                     /translation="MIDYYSLYKNSLEAIGYHKLDTGVDLLQYLIATEEGKRIYSQYP
                     YREVEEIIFYTLDCLIQQDLVTATEMPRLDRRIYTIDGLTPKGMYFLGYIGQVETEVI
                     EWLNEFGVSQNPESIYNALRYIIY"
     gene            2948..3391
                     /locus_tag="VHD02_001809"
     CDS             2948..3391
                     /locus_tag="VHD02_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498391.1"
                     /translation="MAKIYTLTEEELNELVAERMKQAKEKRTPQGLFKDVSFDDELIP
                     INEKYPKVLKKLNRERAYKPEKHVFNQTPKVFGVDNDISYSKITTHDVHNHIRLLVLN
                     VFGKSQNKEVLPEEYDQAIELYNQLKEWFVSSYDKRLEGLVLEDD"
     gene            3507..3929
                     /locus_tag="VHD02_001810"
                     /pseudo
     CDS             3507..3929
                     /locus_tag="VHD02_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
BASE COUNT         1570 a          644 c          745 g         1122 t
ORIGIN      
        1 tgatattatt tttttaccaa caaaacacgg tttaaccgtg taaaatgata gacaactcac
       61 cactagaaag gagaaaagaa aaaatgctac taacggcagc acttattttg attttactaa
      121 cgaatatcgc tatcttaact attattgtca aaatggggaa agaataactg atttatagaa
      181 aggacacaca atggaaatta aagatatgaa agtaagtgtc gatgtaagga ttaaactaag
      241 taattccgaa gcattcagag agatggtacg tgaattcaac acaaaggctc acgaactaag
      301 tttaatagcc catgaacttg agatgtttgt gtttgaaggt gaaatcgaaa atcgagtttt
      361 atcagatgat tgcaatgaac aatcagatct tgcctgaaat atagaaaggg accacatgag
      421 accaaagcga tatccgtatc aaaaaaataa attaattatc cagtcgaaaa acgcaaagac
      481 aaaaattgaa ttagatagcg gaaaaataac tatcaatgct gaagaaatca aggttggtga
      541 tttagcaaac gaaatttcaa aagtgcaact agccaataaa gaaatagctg aaactatcgg
      601 tcaagataaa aagtctactg aaacagtaga ctctaggatt tcacaacttg aaaaagaggt
      661 cgaaggattg aaaggagttg ttggaacagc atatcgactt gctacagaga cttatcatcg
      721 aatttccaaa caatcttcta cctcagtaac ggagtagccg ttgttctgaa gttcctggat
      781 tatttcgtct ttaggaattt taaactcctt aggatatata taagcaacat agtctgttct
      841 gattggatct tcaaatacat attttaaaac agtatcgata gtatcccaat ctttgtaaac
      901 tttttgaatt tcattctttg gagttaattt actcataacc ttccctcctt tccataattt
      961 ttgaatataa cggtgagagg tcatattcaa ataaattata tcagaaagga acgaaagaca
     1021 caacatatag ttatgaatac aaaaacaaaa cacaatatgt tgatttttca atgtagatta
     1081 tgtgggaaaa aattgaacaa ttgatgattg agagaaatct caatatgaac aaattagcaa
     1141 aattgacagg gattcacaaa agccacttta gcgatttgaa aagtggaaga attaaacatc
     1201 tgtcatggcc gaacatggtc aagctatcta caggattaaa agtcagcttg gacgaattta
     1261 aataacaaaa aagtccgacg ggaatcggac tctaaacaaa ctttaattta cttaattata
     1321 ccacgaaaga ggtgataaat ccatgcaatc gcaacttact tacgatctac tcaaaaagca
     1381 gatagcagaa gaactttttg atgagtttaa ggagctcata caagaaaaag atttagcgaa
     1441 tcggtgggtc aaccaaacag cactcgtcaa tgaatacggc tactcatggc aaactataaa
     1501 acgcatggag agctatggcc ttaaatcttt taaaaacggt aaagacaaaa tgtactgtct
     1561 tgctgatgtt aacgaaatta aacacttaat gaaacaataa gcgctgggga gtgctagagg
     1621 agaaacaaca tgaaactatt agattttatt ttcgcaaaac caaaaaaaca ggaagaaaaa
     1681 ccaaaatgga caattgaaaa caacggttgg gaagctaatg cacgtagata taaccaaaag
     1741 cacggtttac ctgctaaaca aatttggtag gaaacacata gtcctttgac aactgaatat
     1801 gggtgcgttg agaaatattt aaaaaagttt gatttagggg ttgacttatg tatccacata
     1861 atgatattat atatttgtgg atacgaaagg aggaaatatg accactacca aaaaacttgg
     1921 tcgtccaact tctaatccta agtctcatag caaaagactg agaatgacag acgaagaagt
     1981 acaaaaatta gaatattgta ctaaacaaac tggaaaaact cagacagatg ttttgatgct
     2041 aggcttagat aaggtctatc aagagctaaa aaaatagcgt ataatccgcc atcgccaaac
     2101 tttggattac acgctatcga ccgacctgaa aacaggtaca caaatatatt atcatgtgta
     2161 cccttttcag tcaacctgac aagggtattt tttgcgccct tgttgtttag ataagggttt
     2221 ttattgtacc caaaaatagg aggaacacaa tggataattc aaaactagaa aatcatcaaa
     2281 aagtaacggt agtctgtaca gattttaatg tctatttgaa tggaatacga cttactggag
     2341 ctagaccaga aaccgctaaa ttagctgaga gatgcggaga gaaactcatt acactaagtc
     2401 tagtagttac agattttgat gaccatagaa cacctaagat ggcggatatg gcaaaggagg
     2461 ctgagatggc taataaaaca ttggactggg agaaaaagca tttctataaa tctaataaat
     2521 aatgtatctc agagcgttat agattgattc tggattttgc gaaactccaa attcattaag
     2581 ccattcgata acctcggttt ctacctgccc gatatatcca agaaaataca tgccttttgg
     2641 tgtcagaccg tcaattgtat atattcgtct atctaatctc ggcatttctg tggcagtaac
     2701 taaatcttgt tggatcaaac aatctagggt gtaaaaaata atttcttcaa cttctcgata
     2761 agggtattga gaatatatcc tttttccttc ttctgttgca ataaggtatt gaagtaaatc
     2821 tacacccgta tccaacttat gatatccgat ggcttcaagt gaatttttgt ataaagaata
     2881 gtaatcaatc atacacttct ccaagtattt tattttaaat ataacaaaat agtaagagga
     2941 aaacaatatg gctaaaattt acacactaac tgaagaagaa ttgaatgaat tagtagctga
     3001 acgcatgaaa caagcgaaag aaaaacgcac accgcaaggg ctattcaaag atgtcagctt
     3061 tgatgatgaa ttgattccaa tcaatgaaaa atatccaaaa gtactcaaga aattaaatcg
     3121 tgaacgtgct tataaaccag aaaaacacgt cttcaatcag acaccaaaag tttttggtgt
     3181 ggacaacgat attagttata gcaaaattac aacacatgac gtacacaacc atattcgttt
     3241 gcttgtccta aatgtctttg gtaaaagtca aaataaggaa gtattgcccg aggaatacga
     3301 ccaagcaata gaactttaca atcaattaaa agagtggttt gtgtctagct atgataagcg
     3361 attagaggga ttggtgctag aagatgatta aaaaattctg cgttaactac ttactaaaac
     3421 agattgacaa aagcaaacta gaaacaagag ataaagcgaa gttgaaccac tttatcacac
     3481 tagtggacta caagttggga ggataaatgg cacagaaatc aaaaacaaaa atctattatt
     3541 ggctgaaatt tgataagcat ttttttgaca atttatttat taaaaggctt ttaagaaatt
     3601 tccctggcgg ttcggaaatg attgttattt acatcaggct catgttagaa gctattgaga
     3661 atgattgcat cattgattac gaaggaactt ttgataatta cgcagaagaa ctagctcttc
     3721 gtcttgaaac ttcagaagaa cagataaata tggctttagc atattttgtg aaatgtggag
     3781 tcattcaggt tgatgatggt ggtaacaccc attatccgca agcaaaggcc ttgttggggc
     3841 aggaaacaaa ctggaataga tacaagaaaa aacaagcaga attggaaaaa ttccaactac
     3901 tttccaacca agttccaaca gagatagaga aagataaaaa gatagatata aatatagata
     3961 taaagtcaga agtagaagaa gagataaaag attcatcttc tgctgctaat gtaaataact
     4021 taaaaattat atctgattat tttcaacaag aaattggcat cttatctccg aatcagtttg
     4081 a
//
LOCUS       DAUDYX010000085         3774 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.086, whole genome shotgun sequence.
ACCESSION   DAUDYX010000085 DAUDYX010000000
VERSION     DAUDYX010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.086"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..1970
                     /gene="ftsH"
                     /locus_tag="VHD02_001811"
     CDS             <1..1970
                     /gene="ftsH"
                     /locus_tag="VHD02_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEQ5498392.1"
                     /translation="KNNGFVKNSFIYILMIIVVITGFQFYLKGTSTQSQQISYSKLIK
                     HLKAGDIKSLSYQPSGSIIEVKGKYEKPKKVTVNSGLSFLGGRASTQVTEFSSLVLPS
                     DTILKEMTAAADKNGTELTVKQESSSGTWITFLMSFLPIVIFAAFMMMMMNQGGGGAR
                     GAMSFGKNKAKSQSKGDVKVRFTDVAGAEEEKQELVEVVDFLKNPKKYKSLGARIPAG
                     VLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAKKAER
                     AIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNENIIVIAATNRSDVL
                     DPALLRPGRFDRKVLVGRPDVKGREAILRVHAKNKPLANDVNLKVVAQQTPGFVGADL
                     ENVLNEAALVAARRNKIKIDASDIDEAEDRVIAGPSKKDRTISQKEREMVAYHEAGHT
                     IVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDLKEQLAGLMGGRVAEE
                     IVFNAQTSGASNDFEQATQIARAMVTEYGMSEKLGPVQYEGNHAMMPGQISPEKAYSA
                     QTAQMIDDEVRELLNQARNQAADIINENRDTHKLIAEALLKYETLDAAQIKSIYETGK
                     MPVDLETDDNEAHALSYDEIKNKMIESESAE"
     gene            2297..3688
                     /locus_tag="VHD02_001812"
     CDS             2297..3688
                     /locus_tag="VHD02_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEQ5498393.1"
                     /translation="MTIFRKKKKYSNKTEMQRHFKVIDLVFLGLGSMVGTGIFTVTGI
                     GAAKYAGPALTISIIISAIAIGILALFYAEFASRMPSNGGAYSYVYATLGEFPAWLVG
                     WYIIMEFLTAISSVAVGWGSYLKGLLANYNIVLPNALNGTFNLKNGTYIDILPVLVMF
                     FVTGIVLMNSKLALRFNSFLVILKFSALALFIFVGIFFIDHNNWSHFAPYGVGQITGG
                     KTGIFAGASVMFFAFLGFESISMAVDEVKEPQKTIPKGIILSLIIVTALYIVVTTILT
                     GIVHYTKLNVPDAVAFALRNIRLYWAADYVSIVAILTLITVCISMTYALARTIYSISR
                     DGLLPKSLYTLTKKNKVPQNATLVTGLLAMICAGIFPLSSLAEFVNICTLAYLIILSG
                     AIIKLRRIEGEPKANEFKTPLVPFLPMLAIIICLSFMSQYKAFTWIAFAIATIIGTLI
                     YLAYGYTHSIENK"
BASE COUNT         1215 a          557 c          746 g         1256 t
ORIGIN      
        1 ataaaaataa tggttttgtt aaaaattctt ttatatatat attgatgatt attgttgtca
       61 tcacaggatt tcaattttat cttaagggaa ccagcacaca aagccaacag attagttatt
      121 ccaaattaat taaacatctt aaggctggag atataaaatc tttaagttat cagcctagcg
      181 gtagtattat tgaagttaaa gggaaatatg agaaacctaa aaaagtgaca gttaattcag
      241 gactatcatt tttaggtgga agagcttcaa cacaggtaac agagttctca tctttagtat
      301 tacctagtga taccatcttg aaagaaatga cagcagcagc tgataaaaat ggaacagagc
      361 ttacagttaa gcaggaaagt tcaagtggta cttggattac cttcttaatg agctttttac
      421 ctattgttat ttttgctgct ttcatgatga tgatgatgaa tcaaggtggt ggaggagctc
      481 gtggtgctat gagtttcggc aaaaataagg caaaatctca atcaaaaggt gatgttaaag
      541 tgagatttac agacgttgct ggtgccgaag aagaaaaaca agaattagta gaagttgttg
      601 attttttgaa aaatcctaaa aaatataaat cactaggagc acggattcca gcgggagttc
      661 ttctagaggg gcctccaggt actggtaaaa cattactagc taaagctgta gcaggtgaag
      721 ctggagtgcc atttttcagt atttcaggtt cagattttgt cgaaatgttt gtcggtgtag
      781 gtgctagtcg tgttcgttct ttatttgagg atgctaaaaa agctgagcgt gcgatcatct
      841 ttatcgatga aattgacgct gtaggtcgtc gtcgtggtgc tggcatggga ggcggtaacg
      901 atgaacgaga acagaccctt aaccaattac tcattgaaat ggatggtttt gagggtaatg
      961 agaacattat cgttattgct gcaactaacc gtagtgatgt tttagatcca gctttactaa
     1021 gaccaggtcg ttttgatcgt aaggttttag ttggtcgccc tgatgttaaa ggacgtgaag
     1081 ccattttacg tgttcatgct aaaaataaac cacttgcaaa tgacgttaat ttgaaagtag
     1141 tagcccaaca gactccaggt tttgtaggtg ctgatcttga aaatgtttta aatgaagctg
     1201 cgttggttgc tgctcgcaga aataaaatta aaattgatgc tagtgatatt gatgaagctg
     1261 aagatcgtgt tattgctgga ccatcaaaga aagatcgcac tatttcgcaa aaagagcgag
     1321 aaatggttgc ttatcacgaa gctggtcata ctattgttgg tttagtttta tctaatgcgc
     1381 gtgtagttca caaggtaact attgtacctc gtggacgagc aggtggttat atgatcgctc
     1441 ttccaaaaga agatcaaatg cttctttcta aagaagatct gaaagagcaa ttagcaggat
     1501 tgatgggtgg tcgtgttgct gaagaaattg tttttaatgc tcaaacatca ggtgcgtcaa
     1561 atgactttga gcaagctact cagatagcaa gggccatggt tacggaatat ggtatgagtg
     1621 aaaagcttgg accagttcaa tatgaaggta accatgcgat gatgccagga cagatctctc
     1681 ctgaaaaagc ttactctgcc caaacagctc aaatgattga tgatgaggtt cgtgaactct
     1741 taaatcaagc tcgtaatcaa gcagctgata tcatcaatga gaatcgagat actcataaat
     1801 tgattgctga agctcttctc aaatatgaaa ctctagacgc tgcacaaatt aaatcaattt
     1861 atgaaactgg taaaatgcca gttgatttag aaactgatga taatgaagct catgcgttat
     1921 cttatgacga aattaaaaat aaaatgatag aatctgaatc tgcagaatga tataaaaaag
     1981 ctgaacttat agattaagtt cagctttttt atcaacacaa tatccttgta aacgttatga
     2041 attgtaatag gtgcctaatt gctagaaatg agaagatgat atactttttt tctttattac
     2101 ttaaagtgag aaagactttg gtatttaaaa ctagaaaatt gttaaagcca tttgaaaaaa
     2161 tctattagtg ttttcttata ataaatagaa gaacagaaga tagcaaaagt ttcagtcaac
     2221 caataagatg aaatttacaa tcattactaa ctatgataca cttagtagaa aatcatttac
     2281 tatagaaggt gataacatga ctatttttcg taaaaaaaag aaatattcca ataaaacaga
     2341 aatgcaaagg cacttcaaag ttattgattt agttttttta ggcctaggtt caatggttgg
     2401 aacaggtatc tttacagtta ctggcatagg cgctgctaag tatgcaggtc ctgctttaac
     2461 aatatcgatt atcatttccg cgattgctat tggaatttta gctttatttt atgcagaatt
     2521 tgcgtctcgt atgccttcta acggtggtgc ttatagttat gtttatgcaa ccttgggaga
     2581 gtttccagca tggcttgttg gctggtatat tattatggaa tttttaacag ctatatctag
     2641 tgttgctgtt ggctggggaa gttatttaaa aggattactt gccaattata atatagtatt
     2701 accaaacgct ttaaatggca cgttcaatct taagaatgga acttacatag atattcttcc
     2761 tgttttagtg atgtttttcg tgacagggat tgtcctaatg aattcgaaat tagccctgag
     2821 attcaatagt tttttagtta ttttaaaatt ttcagcattg gctttattta tctttgtagg
     2881 catttttttt attgatcaca ataattggtc tcattttgct ccatatggtg taggacaaat
     2941 tactggaggt aaaacaggta tctttgctgg tgcttctgtt atgttctttg catttttagg
     3001 atttgaatct atttcaatgg cagttgatga agtgaaagaa ccacaaaaaa caattcctaa
     3061 aggtatcatt ttatctttga ttattgtaac agcactctat atagttgtca ccactatttt
     3121 aacgggaatt gtacactaca ctaaattaaa tgttccagac gcggtggctt ttgctttaag
     3181 aaatattaga ctttattggg cagctgacta tgtgtcgata gttgctattt tgactcttat
     3241 tacggtatgt atttctatga cctatgcatt agctagaaca atttacagta ttagtagaga
     3301 tggtttatta cctaaatcac tttatactct cactaaaaaa aataaagtgc ctcaaaatgc
     3361 tactcttgtc actggactac ttgctatgat ttgtgcggga atatttcctt tatcgagtct
     3421 agcagagttt gttaatattt gtactttagc atatttaatt attctgtcgg gtgcaattat
     3481 taaactcaga agaatagaag gagaaccaaa agcaaatgaa tttaaaactc ctctagtacc
     3541 attcctacca atgttggcaa ttatcatttg tctatcattt atgagccaat ataaagcttt
     3601 tacttggatt gcttttgcta ttgccacaat tattgggaca ttaatctact tagcttatgg
     3661 ttatactcat tcaatagaaa ataaataatg ttttgataat aagatttgac attacattat
     3721 agactataat gtttagaagt aaatgagtag taaaatcaat acctacttac ttat
//
LOCUS       DAUDYX010000086         3768 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.087, whole genome shotgun sequence.
ACCESSION   DAUDYX010000086 DAUDYX010000000
VERSION     DAUDYX010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.087"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            16..816
                     /locus_tag="VHD02_001813"
     CDS             16..816
                     /locus_tag="VHD02_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEQ5498394.1"
                     /translation="MGLTIFKKLVSLIILLLFFAILKRVTNYLFEKTINKSFAYSRQS
                     EARKKTLSKLTHNILNYLLYFLLIYWILSLFGIPVSSLLAGAGIAGVAIGLGAQGFLS
                     DVVNGFFILFENQFEVGDNVTISDIEGSVFGVGIRTTQIRGFDGTLHFIPNRSITVVS
                     NKSRGNMRALIEIPLYSTVNLSQVTRIIDEVNQKELPNHPQIVGKPNILGPQNNSNGQ
                     FTFRIAIFTENGEQFKIYHTFYRLYQEALLKEGIQLPTAISYSITSKS"
     gene            complement(881..1441)
                     /locus_tag="VHD02_001814"
     CDS             complement(881..1441)
                     /locus_tag="VHD02_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEQ5498395.1"
                     /translation="MLNNLEKQTREIVIDVVERSAIQPGNLFVLGLSSSEILGSRIGK
                     QSSLEVGQIVVEVVLDELNKRGVHLAVQGCEHVNRALVVERHVAESKQLEIVNVVPNL
                     HAGGSAQMAAFQFMSDPVEVEEVIAHAGLDIGDTAIGMHIKRVQIPLIPCQRELGGAH
                     VTALASRPKLIGGARADYNMDIIRKS"
     gene            complement(1455..1925)
                     /locus_tag="VHD02_001815"
     CDS             complement(1455..1925)
                     /locus_tag="VHD02_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ5498396.1"
                     /translation="MQNSKIRQMSLTGILTALVVVLGRFVMLPTPTGFLTLLDAGIYA
                     VSFSFGSAQGAIVGGLSGFLIDLVAGYPQWMFHSLIAHSVQGYFAGWRGRKRWLGVVI
                     GSFIMIFWYFLGSLMLGYGLSGSLAGIWGNVMQNTLGLFVGFIIFKAILRQKKR"
     gene            complement(1915..2679)
                     /locus_tag="VHD02_001816"
     CDS             complement(1915..2679)
                     /locus_tag="VHD02_001816"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEQ5498397.1"
                     /translation="MKTDYIVTISGNDILSGGGLYADLATYIRYDLQAFVAVTCLTTR
                     SEEGFSLFPVAKEIFRDQLNSFTNAPISAIKIGLLPNAEMCEIVLDFIKGHLGIPVVL
                     DPVLACKEIDDVKIVPLRQEILQLLPYVTVVTPNLVEAQLLSQKEIVSLKDMQEAAKY
                     FYQLGAKQVVIKGGNRFSQKKAIDLFYDGKEIVTLECPVLEKNNIGAGCTFASSIASQ
                     LVKKKTPLEAVKNSKELVYQAILQSDRYGVKQSYAK"
     gene            complement(2669..3418)
                     /gene="truA"
                     /locus_tag="VHD02_001817"
     CDS             complement(2669..3418)
                     /gene="truA"
                     /locus_tag="VHD02_001817"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEQ5498398.1"
                     /translation="MVRYKATISYDGTLFSGFQRQRHLRTVQEEIEKTLYKLNNGTKI
                     IIHGAGRTDAGVHAYGQVIHFDLPQEQEVEKLRFALDTQTPEDIDVVNIEKVADDFHC
                     RYQKHLKTYEFLVDNGRPKNPMMRHYTTHYPYTLNIKLMQEAINGLVGTHDFTGFTAA
                     GTSVQNKVRTITKATVSRDEKTDFLVFTFSGNGFLYKQVRNMVGTLLKIGNGQMPVEQ
                     VKVILSSKNRQLAGPTISGNGLYLKEICYEN"
BASE COUNT         1207 a          826 c          554 g         1181 t
ORIGIN      
        1 tctcatatcg aaaatattgg cttaacgatt tttaaaaagc tagtgtctct cattattcta
       61 ttactctttt ttgctatttt aaaacgtgtt actaactatc tttttgaaaa aaccattaac
      121 aaatcgtttg cttattcaag acaaagtgaa gctcgcaaaa aaacactctc taagctcact
      181 cacaatattt taaattactt attgtatttt cttctcattt attggatact tagcttattt
      241 ggcatacctg tctctagtct tctagcagga gctggaattg ctggagtagc aatcgggtta
      301 ggtgcacagg gatttttatc tgacgttgtc aatggctttt ttatcttatt tgagaatcaa
      361 tttgaagtag gagataacgt tactatctct gacatagaag gcagtgtctt tggtgttgga
      421 attagaacta ctcaaattcg tggattcgat ggaacactac atttcatccc aaaccgtagc
      481 atcaccgttg tcagcaacaa atctcgtggt aatatgcgtg ccttaattga aattcctctt
      541 tattccactg ttaatttgag ccaagtcact cgaatcattg atgaagtgaa tcaaaaggaa
      601 ttgccaaacc atcctcaaat cgttggcaaa ccaaatattt tagggcctca aaataactct
      661 aatggacagt tcacttttag aattgctatt ttcacagaaa atggagaaca atttaagatt
      721 taccatactt tttatagact ttatcaagaa gctttactca aagaaggcat tcaattaccg
      781 actgcaatca gctattctat cacctcaaaa tcttaatata atcttaaaaa atcctttttt
      841 ctagaaaaaa caccaattgt tttattccag aaaaaagggt ttaggattta cggataatat
      901 ccatattgta atcagcccta gctccaccaa ttaattttgg tcgactagcc aaagctgtca
      961 catgagcacc tcctagctct cgttgacaag gaatcagagg gatctgaaca cgcttaatat
     1021 gcattcctat agcagtatca cctatatcaa gtccagcgtg tgcaataact tcttctactt
     1081 cgacagggtc tgacataaat tgaaaagcag ccatctgagc actgccacca gcatgaagat
     1141 tcggaaccac attgacaatc tctaactgct tagattctgc cacatgcctc tctactacca
     1201 aggcacgatt gacgtgttca caaccttgta cagccaaatg aacaccacgc ttatttaatt
     1261 catctaacac aacttcaaca actatttgac ctacttctag actagattgt tttccaattc
     1321 tactacccaa aatttcgcta gatgaaagac ctagaacaaa aagattacct ggttgaatag
     1381 cagagcgttc tacaacatca atgacgattt cccttgtttg tttctctaaa ttattcaaca
     1441 acttctcact ctccttatct ctttttttgc cttaatatgg ccttaaagat aataaaacca
     1501 acaaaaagcc ctaatgtatt ttgcatgacg tttccccaaa taccggctaa agatcccgaa
     1561 aggccataac caagcataag agagcctaaa aagtaccaaa aaatcatgat aaaagaacct
     1621 atcacaacac caagccaacg cttgcgccct ctccatcccg caaaataccc ttggacactg
     1681 tgagcaatca aactatgaaa catccactga gggtatcctg ccactaaatc gattaaaaaa
     1741 cctgataacc ctcccacaat agctccttga gctgatccaa aggaaaagct gacagcataa
     1801 attcctgcat ccaacagcgt taaaaaacct gttggagtag gtagcatgac aaatcttcct
     1861 aaaacaacaa ccaatgctgt caatatccct gttaaactca tttgtcgtat cttactattt
     1921 tgcataactt tgttttactc cgtatctatc agattgtaaa atagcttggt agactaactc
     1981 ttttgagttt ttcacagctt ctagtggcgt ttttttcttt actaactgac tagcgatact
     2041 agatgcaaaa gtacagcctg cgccaatatt atttttctca agtacaggac attccaaggt
     2101 aacaatctct ttaccgtcat aaaaaagatc aatagccttt ttctgactaa atcgattacc
     2161 acccttgata accacctgtt ttgccccaag ttgataaaaa tatttagcag cttcttgcat
     2221 atcttttaaa gaaacaatct ctttttgaga taatagctga gcctctacca aatttggagt
     2281 aacaacagtt acataaggca ataattgtaa aatctcctgt cttagaggaa ctattttaac
     2341 atcatctatt tctttacatg ctagaacagg atctaaaacc actgggattc ccagatgccc
     2401 ctttataaaa tccaaaacta tttcacacat ttccgcatta ggtaacaaac caattttaat
     2461 agcagaaatt ggagcgttcg taaagctatt caactgatca cggaaaattt ctttagctac
     2521 cggaaataga ctaaagccct cttcagatct agtagttaaa caagttaccg ctacaaaggc
     2581 ctgtaggtca taccttatgt aagtcgctaa atcagcatag agaccacctc cacttaaaat
     2641 atcgttccca gatatagtca cgatataatc agttttcata acaaatctcc ttcaagtaaa
     2701 gcccatttcc agatatcgtt ggtccagcta gctgacgatt tttggagctc aaaatcactt
     2761 taacttgttc aactggcatt tgaccattac caattttcag caaagttccc accatattcc
     2821 tcacctgctt atagagaaaa ccatttcctg aaaaagtaaa caccaaaaaa tcagtttttt
     2881 catcccgaga taccgttgct ttggtaatcg ttcgcacctt attttgtacc gaagttcctg
     2941 ctgctgtaaa accagtaaaa tcgtgtgtac ctaccagacc gttaatagct tcttgcataa
     3001 gtttaatatt aagagtataa gggtaatgag tggtatagtg tcgcatcatc ggatttttag
     3061 gtcgaccatt atcaactaaa aattcataag tttttaaatg cttctgataa cgacaatgaa
     3121 aatcatcagc cactttttct atattaacaa catcaatatc ttcaggagtc tgtgtatcaa
     3181 gagcaaatcg taatttttca acttcttgtt cttgaggcaa atcaaaatgg atcacctgtc
     3241 cataagcatg tactcctgca tccgtacgtc ctgcaccatg aattataatt tttgtaccgt
     3301 tatttaattt atagagtgtt ttttcaatct cttcttgaac cgtacgtaaa tgtctttgtc
     3361 tttgaaaacc cgaaaaaaga gtcccatcat aggaaatcgt tgctttgtat cttaccatgt
     3421 tactatttta acatttctta aacgtaagag aaactatttt taatcaaact gtacccaaac
     3481 aaatgaaaga cgtattgaag cttactctaa gccactagcg atttaaaagc tataagaaag
     3541 ctatttcctc caaactctct ccttttttca tagatttacc ccccaattcc ttataggtct
     3601 tgcttacaac tgctgtgcaa attccttaaa atagggactg atcttcctaa gtagagcacg
     3661 tcttcccttg aaacgttcgc ccctgataag tgcttgaaac tgcatacgct cttgcgaatt
     3721 taattggttc tctaagctag ctattacctc ttgataagcc acatagtc
//
LOCUS       DAUDYX010000087         3764 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.088, whole genome shotgun sequence.
ACCESSION   DAUDYX010000087 DAUDYX010000000
VERSION     DAUDYX010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.088"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..594
                     /locus_tag="VHD02_001818"
     CDS             <1..594
                     /locus_tag="VHD02_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498399.1"
                     /translation="YPQWDGTGAVVDTQVIITDDRGAVISEKVKKDLRTANDAEIIEA
                     ALEEFKKTAYVEIAMGEAVQKVDDLEKISQETAKTAKTAQTAAGLAKVSAERTQKMIN
                     LQTIHVLTSGGKIDPDIYKGMLELIEPAKKGEYKEHDIFTVVDADKDTDGVAGEGNLV
                     FVYVNDKFTYDAQTLKDLEELEDKVTVIKYADLVKQD"
     gene            605..904
                     /locus_tag="VHD02_001819"
     CDS             605..904
                     /locus_tag="VHD02_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498400.1"
                     /translation="MHFDLLQAGAAAAALMSVFGLWKAVVTPFKKAMEANEFAMAQLK
                     DSLKELAYELKNLDRDREITKKIIDRHEERLGRVEDEVIINKERIITLFKKGEEK"
     gene            901..1086
                     /locus_tag="VHD02_001820"
     CDS             901..1086
                     /locus_tag="VHD02_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498401.1"
                     /translation="MSKWFKKVGIKTIKTMAQTAVGLIGSSVLITDINWPTMLSAVLL
                     SGLTCVLMNVSQIKDEE"
     gene            1197..2393
                     /locus_tag="VHD02_001821"
     CDS             1197..2393
                     /locus_tag="VHD02_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEQ5498402.1"
                     /translation="MTFLDNIKQGCLDGWTKYKILPSLTAAQAILESGWGKHAPHNAL
                     FGIKADASWTGKSFNTKTQEEYQPGIVTDIVDRFRAYDSWTDSIIDHGKFLNDNPRYQ
                     SVVGETDYKKACHAIKDAGYATASGYAELLIQLIEENDLQKWDKEILTNQKEVTMTTA
                     NEIVKYCVDLANSGMGVDKDGAYGTQCCDLPCFIVKNWFGIDLWGNAIDLLNSASAQG
                     LEVIYNAPGVNPKASDLFVMEVAGSPYGHTGAVIEDSDGYTIKTVEQNIDGNWDSLQV
                     GGPARFNTRDFTGVVGWIRLPVDHAHQTVDTAPQNSDTIVETPKSGTFTLDVVEINIR
                     RWPSLASEVVGSYKQGDTVSFDSEGYANGYYWISYLGGSGMRNYMAIGITDKDGNIIS
                     LWGKLN"
     gene            complement(2509..3681)
                     /gene="sda1"
                     /locus_tag="VHD02_001822"
     CDS             complement(2509..3681)
                     /gene="sda1"
                     /locus_tag="VHD02_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptodornase Sda1"
                     /protein_id="HEQ5498403.1"
                     /translation="MSKHWRHLIIHSALTILATLFLGILPITENTSSTIYAESGTISN
                     NWSIEQHPNYYHVEGKAQLDIKNFPELYRTTERVYKKSGQSTKPVTVSNIHYSVLDGY
                     GRSGEAYGIITKDMIDMSAGYREKWESKPEPSGWYSYFFKNTNQRATESDYKHSPKNV
                     SKISNNIKASILLSNGNVRNGYLFDRSHLIADSLGGRPFRNNLITGTRTQNVGNNDRK
                     GGMQYIENKVLDHIKRNPKVHVYYKATPVYQGSELLPRAVLVSALSSDGFIDETVRVF
                     NNVAGFNIDYQNGGLLSSTADVDINNVEENEIETTDDEIEEGIENEPDTDALKKDNKD
                     TSLQDTVYVASNGQSDVYWYNKDSMPKTVNLEKVVEMSEQVALTRGKHHSTQENIE"
BASE COUNT         1152 a          691 c          810 g         1111 t
ORIGIN      
        1 tacccacagt gggacggtac aggagcagtc gttgatacac aggtaattat tacagatgac
       61 agaggtgcag tcatctcaga gaaggtcaaa aaagacttga gaacagctaa tgatgcagaa
      121 attatcgagg ctgctttaga ggagtttaaa aagactgctt acgtcgaaat tgcaatgggc
      181 gaagccgtgc aaaaagtaga cgaccttgaa aaaatctcac aagaaaccgc taagactgcc
      241 aagactgctc aaacagccgc tggactagct aaagtgtccg ctgaacgcac gcaaaagatg
      301 attaacttgc aaaccatcca cgttttgaca agcggtggca agattgatcc tgacatttat
      361 aaaggcatgt tagagctcat cgaacctgct aaaaaaggtg agtacaaaga gcatgatatt
      421 tttacagttg tagacgctga taaagatacg gacggtgtag ccggtgaggg gaacttagtc
      481 tttgtttatg tcaatgataa gtttacttat gatgcacaaa cactcaaaga tttagaggaa
      541 ttagaggata aagtcacagt tatcaagtac gctgatttgg ttaagcagga ttagaggtgg
      601 ttagatgcac tttgatttac tacaggcagg agctgcggca gcggctctca tgtctgtttt
      661 tggtctttgg aaggcagttg taacgccttt taaaaaagcc atggaagcta atgagtttgc
      721 catggctcaa ctcaaggact ctctcaaaga gttagcttat gagcttaaaa atcttgatcg
      781 cgatcgtgaa attaccaaaa aaattatcga tcgtcacgaa gagcgtttag gtcgcgtcga
      841 agacgaagtt attatcaaca aagagcgtat tatcacgcta tttaaaaaag gggaagaaaa
      901 atgagcaaat ggtttaaaaa agtaggaatt aaaacaatca aaacaatggc acaaactgcg
      961 gttggtctta tcgggtcaag cgtgttgatt acggatatta actggccaac aatgttgtca
     1021 gcggtactgc tatcaggact aacatgtgtc ctgatgaacg tgtcacaaat taaagacgag
     1081 gaataagcca tgcgagcaat cacacgatta gcgttaatac tagcaatcgc aatactgtat
     1141 gtgccattat ctgtggttgc tcttatcttt tacccgtttg tagataagga ggacagatga
     1201 cctttttaga taacattaag caaggctgct tagatggctg gactaagtac aaaatcttgc
     1261 catccttgac cgcagcacaa gcaatcttag agagcgggtg gggtaaacat gccccacata
     1321 acgctctgtt tgggattaaa gctgatgcaa gctggacagg taagtctttt aacactaaga
     1381 cgcaggagga gtaccagcct ggtatcgtca cggatattgt ggaccgattt agggcctatg
     1441 atagctggac tgatagcatt atcgaccatg gcaagttttt aaacgataat ccacgctatc
     1501 agtctgttgt tggtgagact gactataaaa aagcctgtca cgctatcaag gacgcaggtt
     1561 atgccacggc aagtggatat gcggagctgc ttatccaact aatcgaggag aatgacctac
     1621 aaaaatggga taaggaaatc ttaacaaatc aaaaggaggt aacgatgaca accgcaaatg
     1681 agattgtaaa atactgtgtc gaccttgcca attcaggtat gggagttgac aaagacggtg
     1741 cttatgggac gcaatgctgt gacttaccat gttttatcgt caaaaactgg ttcggcattg
     1801 atttatgggg taatgccata gacctgttaa atagcgcatc tgcgcaaggg ctagaggtca
     1861 tatataatgc ccctggagtc aatcccaaag ctagtgacct ttttgtcatg gaggtagctg
     1921 gtagtcccta cggacataca ggagctgtca tcgaggatag tgatggctat actattaaaa
     1981 ccgttgagca aaatattgac ggtaattggg atagcttaca agtaggtgga ccagcccgtt
     2041 ttaatactcg tgattttact ggcgttgtcg gctggattag gttacctgtt gaccatgctc
     2101 atcagacggt agatacagca ccacaaaact ctgacacaat cgtagagaca ccaaaatctg
     2161 gtacctttac gcttgatgtt gtggagatta atattagacg ttggccaagc ctagccagtg
     2221 aagtagtagg tagctacaaa caaggagaca ctgtcagctt tgacagcgag ggttatgcca
     2281 atggctacta ctggattagt tatcttggcg gctcaggtat gcgaaattac atggctattg
     2341 ggataactga caaagatggt aacattatca gcctttgggg taaattaaat tagataagac
     2401 aaaaccgctc tcttgattga gggcggtttt ttgtgtgttt cgtatgctta tgcataacta
     2461 ataagaatat aaaaactatt tcaagttcta gttggtctgt tgaatttgtt attctatatt
     2521 ttcttgagtt gaatgatgtt tacctctagt caaagctact tgttcactca tttctacaac
     2581 tttctctaag tttacagttt taggcatact gtctttgttg taccagtata catcagattg
     2641 cccattactt gccacatata cagtgtcttg taaagaagta tctttgttat ctttttttag
     2701 tgcatccgtg tcaggctcgt tttcgattcc ctcttcaatc tcgtcatcag tagtttcgat
     2761 ttcattttct tcaacgttat taatatctac gtcagcagta gaagataaga gtccaccgtt
     2821 ttggtaatca atattaaaac ctgctacatt attaaacaca cgaactgtct cgtcaataaa
     2881 tccatcagat gataaagcag acactaaaac tgctctaggt agcaattcgg atccttgata
     2941 tacaggagtt gctttatagt aaacatggac tttaggattt cttttaatgt gatctaaaac
     3001 tttattttca atatactgca tcccaccttt acgatcatta ttacctacgt tttgggtgcg
     3061 ggtacccgta atcaaattat ttctaaaagg tcttcctcct aagctatcgg ctattaaatg
     3121 acttctatca aacaggtagc cgttacgaac atttccgtta cttaagagaa tactagcttt
     3181 gatattgttc gaaatcttac tcacattttt ggggctatgc ttgtagtcgg attcagtggc
     3241 tctctggtta gtatttttga agaaataaga ataccaccca cttggctctg gtttgctttc
     3301 ccatttttca cgatagccag cagacatgtc aatcatatct tttgtgataa taccataagc
     3361 ttctccagaa cggccgtagc catcaagtac agagtaatgg atattggaaa ctgtaacagg
     3421 tttagtactt tgcccacttt tcttatagac cctttcggtt gtacgataaa gttcgggaaa
     3481 atttttaata tccagttgcg ctttaccttc aacatggtaa taattggggt gttgctcgat
     3541 actccaatta tttgaaatag tgcccgattc agcgtaaatg gtactactag tattttcagt
     3601 aattggtagt atacctaaaa ataacgtagc tagaatggtc agagcagagt gaataattag
     3661 atgtctccaa tgtttagaca tatttatgtc ctccttttgt tatttacaga ttaagtatac
     3721 cattttaaat taaaaaaata tgaaattaat aaaattaatt tata
//
LOCUS       DAUDYX010000088         3684 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.089, whole genome shotgun sequence.
ACCESSION   DAUDYX010000088 DAUDYX010000000
VERSION     DAUDYX010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.089"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            66..665
                     /locus_tag="VHD02_001823"
                     /pseudo
     CDS             66..665
                     /locus_tag="VHD02_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein"
     gene            840..1165
                     /locus_tag="VHD02_001824"
                     /pseudo
     CDS             840..1165
                     /locus_tag="VHD02_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670032.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            1156..1430
                     /locus_tag="VHD02_001825"
                     /pseudo
     CDS             1156..1430
                     /locus_tag="VHD02_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883480.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1476..1688
                     /locus_tag="VHD02_001826"
     CDS             1476..1688
                     /locus_tag="VHD02_001826"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498404.1"
                     /translation="MMTDRVMKKPIRGRQLSKRDLYDEEFFRTHFAKIEIESRLEFLL
                     NRANSLEELLLKAKEVNLTIDLKQKM"
     gene            1953..2650
                     /locus_tag="VHD02_001827"
                     /pseudo
     CDS             1953..2650
                     /locus_tag="VHD02_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic (srt) production protein"
     gene            2801..3487
                     /locus_tag="VHD02_001828"
     CDS             2801..3487
                     /locus_tag="VHD02_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ5498405.1"
                     /translation="MYKILAIDDDKEILKLMKTSLEIENYHVITCQEIELPIVFDDFK
                     GYDLILLDIMMPNISGTEFCYKIREEVHSPIIFVSALDGDNEIVQALNIGGDDFIVKP
                     FSLKQFVAKVNSHLKREERAKIKNEAEERVKRSFPPIEIYLEERMLYIDKQPLFLTYR
                     EYEILELLSRHPYKVFTKEEIYEQVYSDEASALFHSISEYIYQIRMKFSSFGINPIKT
                     IRGIGYKWDV"
BASE COUNT         1300 a          519 c          760 g         1105 t
ORIGIN      
        1 atttttttat tttcaggagg aaaatcatgc aaatgaatga acagtcatca cgacaaatgg
       61 tgattgccat ctatctcatt tattccaact caaaatgata gtactccttg gctgttccaa
      121 gtcgaaattg ttagtgatta agaaaaccta cgagactatg gtttgatcga tgaaggccag
      181 cagtctccca gtgaaaaagg gcgtttggct aataagattt atttaggaaa gttggaacat
      241 gatttcaccc cagtcttaag ttcagacggg gccagtctca aaaacagacc agccccgtgt
      301 taaattcagc ccctagtgag actgaaaaga gtgaaactga aggtagtgaa actaaagaga
      361 gtaatttagt cattgaggac gaggaggaaa gaaaggagtg cactagtgtg aaaaagactg
      421 agggtcactt tactcgtcaa gttgatcatg tcaccaaata tgacaaagat tatatttgga
      481 gtttggttca ttcccaatta cgtgaaggtg gtttatcaca agctgctagt gacttagtca
      541 tgagctattt tgaagaacgc tatgcttatg cgctggaaca catacggttc gcaagaacag
      601 ctgaagctat tgcggaatat gtctttaatg gtgtcttatc cgaatggacc aagcaactaa
      661 ggtgacaaga agcgaagtga gtaagatgtt ttggtggcta ttattaggtg tttggggact
      721 aaggacaatt tggttgatta ttgaagttat cactgcacca gaaatggaag attatagttg
      781 agtaaggacg ttacataagc agtaaggtcc ttttattttg tcagaaagga atgagagata
      841 tgcagttttt agatttgttt gcaggaattg gaggctttcg attgggttta attaatcagt
      901 gccatgagtg tattggcttt tgtgagattg ataagtttgc gaggcaatct tacaaagcca
      961 tctatgagac cgaaggagaa atagaatttc atgacattag acaagtcaca gaccaagact
     1021 ttagacaact tagagggcaa gtggatatca tctgcggtgg tttttccctt gtcaagcatt
     1081 ttcactcgca ggcagacgac tgggatttga gggcactagg agaactctct tctttgagat
     1141 tactcgagcg accaaacaga taaaaatcat acccataatc acatccaatc aacgccattg
     1201 accgtaattc agataaaaaa ttgatatgga attatgcctt ggaacgaaac ctccgtatga
     1261 tttcagaccg tatttcaaaa atgactggag caaaaattat cgagaagcgt tattcctacc
     1321 gtgactatca aaaatatagg cagtctagtc ataaatttga attgaaacaa cgtctttatt
     1381 ttttgatgca gcagtcaaag ttctttgatg attttttaga aaaagcagag taattacatg
     1441 ttcatattga ttttagtcag aagcatagca gattcatgat gacggatcga gtaatgaaaa
     1501 aaccaattcg aggccgtcaa ctcagtaaac gagatttata tgatgaagaa ttttttcgta
     1561 cacattttgc caagatagaa attgaaagtc gattagaatt tttgttgaac cgtgccaatt
     1621 cattagagga gctactatta aaagcaaaag aagtgaatct aaccattgac ttaaaacaaa
     1681 aaatgtaact tttatcctag aagaagatac tcaaaagata agtttaggtc ataaaaaaat
     1741 aagcgataag aaattatatg atgtcaattt tttcaagatt actttaaaaa taaggaagtc
     1801 ggtgtttcag atcgattaga aagtctacag aaacagtaca tgcttttcaa gaagaacgag
     1861 ataaagagga ggtatccact gaagagagta aggaagtctt caagaaatac aaggggcaac
     1921 gagatgccat ccatgagttt gaagttgaac ttgtggaaca tcaaattgag aggttaattg
     1981 ctgaggacat ttatattaag gtgtcttttg gtgtaaagca gagtggtctt attttcattc
     2041 ctaactatca attggatatt aggaaagaag aaaatcataa aaaatataaa gtctatattc
     2101 gtgagaccgc acaattcttc atttacaaca aggaggcgtc taaacttaat cgctatatgc
     2161 gagggcgtga gctcatttac cagttaacca atgatagcaa gagtattcca aaaagaagac
     2221 gaccaaaacc attgatacac ttaagaaaaa gattgaagaa ataagtctcc tcattgagtt
     2281 agatacggag aataagcctt atcaagatat taaagatgac attgttaaag atatggctca
     2341 attagatttg accattacag agttgcagga ccatattgct catctcaata aggttgcgga
     2401 ggtcttgctt aatatgaaca acaacgatat agagaaccgc cgtttggcca gatatgacta
     2461 tgccaagatg aacttgactg cagctataaa aatagaagaa gttgagaaag agattgaaac
     2521 ttctcaaaat gaacttaata tatccataga tgagtatgaa tatctagtaa gaaggttgga
     2581 aaagtttgga gagatcttga gttatagcaa aattatcgat acttctcgaa atgaaataca
     2641 atgggagtaa ttatgatata atttgggtgg tagcaatttt tatatcagat attaaaatct
     2701 agttgatgtt tcgtaaataa ctatattaaa gaaatgattc ctgccgctta cattgagaat
     2761 agtttatttg taagtatttc attatttaaa aggggtaata atgtataaga ttttagccat
     2821 agatgatgat aaagaaattc tgaagcttat gaagacttct ttggagattg aaaattacca
     2881 tgttataacc tgtcaagaga tagaattacc tatagttttt gatgatttta aaggatatga
     2941 tttgatctta ttggatatca tgatgcctaa tataagtgga actgagtttt gttataaaat
     3001 tcgggaagaa gttcattctc caattatttt tgttagcgct ttagatggcg ataatgaaat
     3061 tgtccaagct ttaaatatag ggggagacga ttttattgtg aaaccattta gcttaaaaca
     3121 attcgtagct aaagttaact ctcatttgaa gagagaagag agagcaaaga taaagaatga
     3181 ggctgaggag agagtgaagc gcagttttcc acctatagaa atctatctag aggaacgtat
     3241 gttatatatt gataaacagc cgttattcct gacttataga gagtacgaaa ttttagaatt
     3301 actgtcacgt catccgtata aagttttcac aaaagaagag atatatgaac aagtatatag
     3361 cgatgaagct tcagcattgt ttcattctat ttcagaatat atctatcaga ttagaatgaa
     3421 attttcaagt tttggaatta atccaataaa aactattcgc gggattggtt ataaatggga
     3481 tgtttaaaaa atattcaata aaaaatcgtg tttggcgcgc cgtcgttgaa attgtctttg
     3541 gagtttgtat cactgtttta atgattgcga taatatcgct ttctttttca aagttaaata
     3601 tagtgacatc tcataatgtg ggagaagaat tttatattaa agataaacag tcaatcaaac
     3661 agttgaacaa ttatatgaag acat
//
LOCUS       DAUDYX010000089         3488 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.090, whole genome shotgun sequence.
ACCESSION   DAUDYX010000089 DAUDYX010000000
VERSION     DAUDYX010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.090"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(72..611)
                     /locus_tag="VHD02_001829"
     CDS             complement(72..611)
                     /locus_tag="VHD02_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEQ5498406.1"
                     /translation="MFTTKELVKVAMMTTLIIILGFIPAIPLGFIPVPIVLQNLGVML
                     AGLMLGGKKGTLSVFLFLVIGLFLPVFSGSRTTIPVLMGPSAGYVIAYLLVPIVFSLL
                     YRNWFSKSTPLAFLALLISGVVLVDVLGAIWLAAYTGMSLVTSLLSNLVFIPGDTIKA
                     IIATIIAVKYKDSFLNTKQ"
     gene            complement(615..926)
                     /locus_tag="VHD02_001830"
     CDS             complement(615..926)
                     /locus_tag="VHD02_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498407.1"
                     /translation="MTDCFGIDLDQEYRCLHYHTPLDIVGLKCAFCQTYYACYHCHDQ
                     LTDHAFVPTGHQETSPVICGHCRKLLSLAEYGCGCCPYCQSPFNPACHRHKDIYFLKE
                     S"
     gene            complement(1144..2286)
                     /gene="tgt"
                     /locus_tag="VHD02_001831"
     CDS             complement(1144..2286)
                     /gene="tgt"
                     /locus_tag="VHD02_001831"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEQ5498408.1"
                     /translation="MTDYPIKYRLIKTEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKAIGSGIILSNTYHLWLRPGDELIARSGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAADLVAMDFPGYSI
                     GGLAVGESHEEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSEGRLVIKNAKFAEDFTPLDHDCDCYTCQNYSRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKKVRQAIMDDNLLEFRQDFLERYGYNKSNRNF"
     gene            2505..3368
                     /locus_tag="VHD02_001832"
     CDS             2505..3368
                     /locus_tag="VHD02_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEQ5498409.1"
                     /translation="MSIKAIKGQARDTLKNLSGKYLLFLIPTLLFMFHFGIEIHQGYV
                     LSSGIEVSLAASYFPLLLGLILSLFILSASFTMIDVVRHFRQKVSFAESTTAFSKEFF
                     GNLLVLAITKWLFFLIWSLIWFFGLFIFLSGLSAFLVNAKSGGSTVISLIFLLFGAVL
                     SLIGFGIYINRYYAYSLSEYLLYDKVKEGTYLGAIAVIETSVAMMKGYKWKLFFLQLS
                     FTGWFLLNIVTFGLLNIYLLPYFTTANVIFYDQLKKRFKDKDDPIEGEHLSLHQLNKK
                     PTPMMYDDQKD"
BASE COUNT         1091 a          704 c          658 g         1035 t
ORIGIN      
        1 accaaaaaca ggtcctagca gataaatctg ccaagacctg ttcaacacaa cttaactatt
       61 tgatgattag attactgttt tgtattcaaa aaactatctt tgtatttaac agcgataatg
      121 gtcgcaatga ttgctttgat ggtatctcct ggaataaaga ccaagtttga caaaagcgat
      181 gtcacaagag acatgccagt gtaggctgcc aaccagatag cacccagaac atcaaccagt
      241 acaactcctg agattaaaag agctaggaat gccaatggcg tgctttttga gaaccagttg
      301 cggtaaagta atgaaaagac aataggcaca agaaggtagg caataacata gccagcagat
      361 ggccccatca atactggaat ggttgttcgt gagcctgaga aaacaggtaa gaaaaggcca
      421 atcactaaaa ataagaaaac agataaggtt ccttttttac cacccagcat caatccagcc
      481 aacatgacac ctaagttttg taaaacaata gggactggga taaagcctag cgggatagca
      541 ggaataaagc ctaaaataat aatcaaagtg gtcatcatag ccaccttaac aagttctttg
      601 gttgtaaaca tctgttacga ctcctttaaa aaatagatat ccttgtgcct atgacaggct
      661 ggattaaagg gactttgaca ataaggacag catccacaac catactcagc taggctcaag
      721 agctttcgac aatgcccaca aataacagga gatgtttctt gatgtcctgt tggcacaaag
      781 gcatgatctg ttaactggtc atggcaatgg taacaagcat aataagtctg acaaaaagca
      841 catttgagcc caacaatatc aagcggggta tgataatgta ggcaccgata ctcttggtca
      901 aggtcaattc caaaacagtc cgtcatatct catcctcttg taacagttta atcaaaagct
      961 tatgatttgt caactatatt tttacaaaag ggtaactgtt ttctgaaaaa gaacacgtaa
     1021 aacaagaact tccctttaaa gagtgaccta tcagttgaaa gcaataggac atccgtctac
     1081 acaaaaaaac ctgaaaccaa agacgataga tcgccctcag ttccaggatc atagcatttt
     1141 taattagaaa ttacggtttg atttattata accataccgt tcaagaaaat cttggcggaa
     1201 ttcaagtaag ttatcgtcca taatagcctg acgtactttt ttcatcaagt taaccaagaa
     1261 ataaaggttg tggtagcttg tcaaacgaat accaaaagtt tcgtccgctt ttagcaagtg
     1321 acgaatgtag gcacggctat agttttgaca agtgtagcag tcacagtcat gatcaagtgg
     1381 ggtaaaatct tcagcgaatt tggcattttt aatcacaaga cgcccctcac tagtcatgca
     1441 agttccgtta cgggcgatac gcgttggcaa gacacagtca aacatatcta caccacgaat
     1501 cacaccatca atcaagctat ctggtgctcc cactcccatc aggtaacgag gtttattttc
     1561 tggtaagagg ggagtcgtaa agtcaaggac ggcattcatt tcttcatggc tctccccaac
     1621 agctaaacca ccaatagaat aacctgggaa atccatagcc accaaatctg ccgcagactg
     1681 acgacgaagg tcctcaaatc cagctccttg tacaattcca aataatcctt ggtcatgagg
     1741 acgacgatgg gcttttaagc cacgttctgc ccaacggcta gtacgttcaa tggatttttt
     1801 cacgtaatca taaggttggt aaaattgcgg gcactcgtca aaactcatca tgatgtctga
     1861 gccaaggtta ttctgaatgg aaatggcttt ttcgggtgat agaaacattt ttgagccatt
     1921 caagtgattt ttgaaagtca ctccttcttc agtgatgttt cgtgaatcgg ctaaagagta
     1981 aacttgaaaa ccacccgaat cggttaagat tggctgatcc cagttcataa acttgtgcag
     2041 accaccagag cgagcaatca actcatctcc tggacgcaac cagaggtgat aagtgtttga
     2101 taaaataatc cctgatccaa ttgcttttaa ctcttctgga gactgtgttt taacagtagc
     2161 ctgagttccc actggcataa acattggtgt cggaaaggtc ccgtgagggg tgataatttc
     2221 gcccaagcgt gcaccggtgt gcttttctgt tttgataagg cgatacttaa tcggataatc
     2281 tgtcatgtgc tttcttcctt gctatcaaga taaacagtct tgagcttttt tgatctgcct
     2341 gagtttccat cataatgaag ggcttcaaga tgacatgttg ttctagcgcc tatcatttta
     2401 tcaaaaaatc cataatttgt catgctttcc caaaaaagta tagtcaaaat aatggttagc
     2461 tgtctcattt tgttttatgg tataatgaaa taaaggagtc tgttatgtca attaaagcaa
     2521 tcaaaggcca ggctagagac acgttaaaaa atctctctgg caagtattta ctatttttaa
     2581 ttccaaccct tctctttatg tttcatttcg gtattgagat tcatcaaggt tacgtcctat
     2641 ctagtggtat tgaagtctcg ctagcggcta gttatttccc attactacta ggacttatcc
     2701 ttagcctttt cattttatca gcatccttta cgatgattga tgttgttagg cattttcgtc
     2761 agaaagtttc ttttgccgaa tcaacaacag ccttctcaaa agaattcttt gggaacctgc
     2821 tggttttagc gatcactaag tggctattct ttctcatctg gtcactaatt tggttttttg
     2881 gcctctttat tttcctttcc ggactttcag cgtttttggt caatgctaaa tcaggtggct
     2941 caacagtcat ttcactgatc tttttactat ttggagcagt tttgtcctta attggttttg
     3001 gtatttatat caaccgttat tacgcttata gtctatcaga ataccttctc tacgataagg
     3061 ttaaagaggg gacttatcta ggagctatag ccgtcattga aacaagtgta gctatgatga
     3121 aaggttataa atggaagctt tttttcttac aacttagctt caccggttgg tttctactga
     3181 atattgttac ttttgggtta cttaatatct atcttttacc atattttaca acagccaatg
     3241 tcatttttta tgatcaactc aagaaacgtt tcaaagacaa agatgaccca attgaaggag
     3301 aacatttaag ccttcatcag cttaataaaa agcctactcc tatgatgtat gacgatcaaa
     3361 aagactaaat aaatcctcca tccataaaaa tagcccttga actgcacccc aaaagttaga
     3421 cacaaaatct aacgattggg gtgctatttt tttatgaaat ttaatcaaga aacgaaagtt
     3481 aagattta
//
LOCUS       DAUDYX010000090         3246 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.091, whole genome shotgun sequence.
ACCESSION   DAUDYX010000090 DAUDYX010000000
VERSION     DAUDYX010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.091"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..1617
                     /locus_tag="VHD02_001833"
     CDS             <1..1617
                     /locus_tag="VHD02_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ5498410.1"
                     /translation="FHTLMIAFISYIALTLLTIIIGEVDSYIDTKLQILLHYKMNHLV
                     MQKTVKLTLAEFETPEIYDDITRIQNQISYKPFQIYKSIISVLSSLVSLISSFVILLN
                     WKISIFPLLLILPIVSIYIYLKIGKNEFEILYKRSSDERANWYISHLLTHDFAVKELR
                     LGTLESYFLKKYDKNNQLFINQENSINRSKMKYGIVLGVLEIAIQSIIMFLAVREAFL
                     GILLVGNVTTFIRSLSTMQTSTQNIVNNIYSFYNGSLYMELLYDFTHDELVHNPIIES
                     MVDTIQSVEFERVSFSYNGKDNVINDISFSIKAGERLAIVGENGSGKSTIFKLACGLY
                     DNYEGNIYINGINLRLIQKKSYYKRISALFQDFLKYELTLRENVGLGELSKLYSDEDL
                     ISALKYTGVDSIFYFNQEKLNQIDLEQQLGNWFEDGRQLSGGQWQKIALSRVYLKEAD
                     CYLLDEPSSALDPESETKIFKTFFELSQNKIGIFITHKPSITQSVNKILYLDNGCIAE
                     TGSFADLNIKGMKFKNLLDKEIGATYDTSN"
     gene            1601..2845
                     /locus_tag="VHD02_001834"
     CDS             1601..2845
                     /locus_tag="VHD02_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HEQ5498411.1"
                     /translation="MTPQIKQIVDYIADSFINYEQIVAESENKVLINIESKYRNTFFD
                     KSLSHGVPSLILMYSSLYKVTKDERYLTFSNIYVEKLVDIISRDGIESPSLYAGTAGI
                     ALAIKEASISGKYYPKLLSSLNCLLKEQIKDKLVVSFSNIEKGIIEPYDYDMVNGFSG
                     ITNYLLLEQEFFSVELNQIGNYLLKYIETILNKVTNCSEDREAEFDLGIAHGITGPML
                     ILAKLKSEKILSVEVGDILNKAINLMFLFRRDDKLWPGKIYTSNILDLDLNYLPTRMA
                     WCYGTPGTAVALFKMCQLIDLNFIDDINESLIAQMRSSDKNVYSLTMCHGLAGVTFIY
                     DLIGKNYSSERLTRFAEELRQQIITSFSTTKVFGYTDREKFGEIEVELESIGILQGVS
                     GIVLFLLNPYSDEELLWQRIFI"
     gene            2864..>3246
                     /locus_tag="VHD02_001835"
     CDS             2864..>3246
                     /locus_tag="VHD02_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEQ5498412.1"
                     /translation="MYKVFPTGMIRIPLQKQEFYNKDYEDLINDKVFMEQLLVASPSL
                     YDSVIDFDKCNEKKKQNVKVSLNSYSKRAAFRTTPFGIFTAINTVDLTKGTTSNVQKV
                     SFIKKAVPDYSWIYSLVKSYEISNL"
BASE COUNT         1188 a          381 c          567 g         1110 t
ORIGIN      
        1 tttcacacat tgatgattgc ttttatctca tatattgctt taacattatt gacaattata
       61 ataggagaag ttgatagtta tattgatacc aaattacaga tacttcttca ctataagatg
      121 aaccatttag ttatgcagaa aactgtaaag ttaacattgg ctgaatttga gacaccagaa
      181 atctatgatg atatcactcg catacaaaat caaatatctt ataaaccttt tcaaatatat
      241 aagtcaatta tttctgtatt atcttcgtta gtatcattaa tttcctcatt tgttatttta
      301 ttaaattgga agatatcaat ttttccactt ttacttatcc ttcctatcgt ttctatatat
      361 atctacttga aaattggtaa aaatgaattt gagatactat ataaaaggag tagcgatgag
      421 agagcaaatt ggtatattag tcatctttta acacatgatt ttgctgtcaa ggagttgagg
      481 ttaggtacgt tagaaagtta ttttctaaaa aaatatgata aaaataatca attatttatt
      541 aatcaggaaa atagtataaa tagatctaaa atgaaatacg gcatagtatt aggtgtattg
      601 gagattgcta tacaatctat tattatgttc cttgcagtta gagaagcatt tctaggaata
      661 ttattagttg gaaatgtaac tacttttata aggtctttat ctacaatgca aacaagtaca
      721 caaaatatag taaataatat ctattctttc tataatggtt cattgtacat ggagttattg
      781 tatgatttta cgcatgatga gttagtacat aaccctataa tcgaaagtat ggttgataca
      841 attcagtcag ttgaatttga aagagtttct ttttcatata atggtaagga taatgtaata
      901 aacgatataa gtttttcaat taaagcagga gagaggttag caattgttgg tgaaaatggt
      961 tctggaaaaa gtacgatttt caaattagcc tgcggtcttt acgataatta tgaaggcaat
     1021 atatatataa atggtattaa tcttcgttta atacagaaaa agtcatacta caagagaata
     1081 tccgcattgt tccaagattt tttaaagtat gaattgacac tgcgtgaaaa tgttggattg
     1141 ggtgaattat caaagttata tagtgatgag gatttaataa gtgcactgaa gtatacagga
     1201 gttgattcga tattttactt taatcaggaa aaattaaatc agatagatct ggaacagcaa
     1261 ttagggaatt ggtttgagga tgggagacag ttatctggtg gacagtggca aaaaattgca
     1321 ttatcaagag tatatctcaa agaagcggat tgctatttgc tagatgagcc tagttctgcg
     1381 ttagatcctg agtctgaaac aaaaattttt aaaacatttt ttgaattatc gcaaaataaa
     1441 attggtattt ttattacgca taagccaagc attactcaat ctgtgaataa aattttgtat
     1501 ctagataatg gatgtatagc tgaaacagga agtttcgctg atttaaatat taaaggaatg
     1561 aagtttaaaa acttattaga taaagaaatt ggagccacat atgacacctc aaattaaaca
     1621 gatagtagat tacattgcag atagttttat aaattatgaa cagatagtag ctgaaagtga
     1681 gaataaagta ctaattaata ttgaatctaa atatagaaat accttttttg ataaatctct
     1741 gagtcatggt gtgccatcat taatcttaat gtatagttca ctatataaag tgacaaaaga
     1801 tgagagatat ctaacattct ccaacatata cgttgagaaa ttagtagaca taatctctag
     1861 agatggtata gaatctccgt ctctctatgc tggcactgca ggtattgcat tagctattaa
     1921 agaagcgagt atatctggaa agtattatcc gaaattatta tcatccttaa attgtcttct
     1981 aaaagaacaa attaaagaca aattggtagt aagtttttct aatattgaga aaggaattat
     2041 tgagccatat gattatgata tggttaacgg atttagtgga ataactaatt acttactcct
     2101 tgaacaagaa tttttttctg tagagttaaa tcaaattgga aattatcttt tgaaatatat
     2161 tgagacaatt ttaaataagg taactaattg ttctgaagat agagaagctg aatttgatct
     2221 aggaattgca catggaatta ctggtcctat gctaatccta gcaaaattaa aatcagaaaa
     2281 aattttgagt gtagaagtag gagatatact gaataaagct ataaatttaa tgtttttatt
     2341 tagaagggat gacaaactat ggcctggaaa aatttatact agtaatattt tggatcttga
     2401 tttaaattat cttcctacta gaatggcatg gtgttacggt acgcctggaa ctgcagttgc
     2461 cctttttaaa atgtgtcaat tgatagattt aaattttatt gacgatatta atgaaagttt
     2521 aattgctcag atgagaagtt cagacaaaaa tgtctattct cttacaatgt gccatggttt
     2581 ggcaggagta acttttatat atgacttgat tggtaaaaat tattcaagtg agaggctaac
     2641 tcgctttgca gaagagcttc gccaacaaat aattacatcg ttttcaacta caaaagtatt
     2701 tgggtatact gacagagaaa aatttggaga aatagaagtt gaactggagt caatagggat
     2761 attgcaaggg gtatcaggga tagtgttatt cttattaaat ccttacagtg acgaggagct
     2821 attgtggcaa agaatattta tttaatttga aaaggagaac aaaatgtata aggtatttcc
     2881 aactggtatg attcgtattc cattgcaaaa acaagaattt tataataaag actatgaaga
     2941 tttaataaat gataaagttt ttatggaaca acttttagtt gctagtccca gtctttatga
     3001 tagtgtaata gattttgata aatgtaatga aaagaaaaag caaaatgtaa aagtctcatt
     3061 aaatagttat tctaaaagag cagcgtttag aactactcca tttggtatct ttactgctat
     3121 taatacagtt gatttgacaa agggaaccac ttctaatgta caaaaggtta gttttattaa
     3181 aaaagcagtc ccagattatt catggatata ttctctagta aaatcgtatg aaataagtaa
     3241 ccttga
//
LOCUS       DAUDYX010000091         2950 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.092, whole genome shotgun sequence.
ACCESSION   DAUDYX010000091 DAUDYX010000000
VERSION     DAUDYX010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.092"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..688
                     /locus_tag="VHD02_001836"
     CDS             <1..688
                     /locus_tag="VHD02_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEQ5498413.1"
                     /translation="NLKERLQKYNITPISIASPLFKWNIEENHLESNLDLYGISANVT
                     LNEQWLYVRKIIFIAKFLNIKYVRIFGGIGCSVDDFLNNELFLNLLKETEIIFLIENE
                     LGTSVSTADDLLKIGHFIDEKSIKNLKIWFDIANYYIVEPQVDKVIPKLKKYIYYIHC
                     KNYVNNDLGIHYVELGKGIIDYKTIIHEIHEHFIDLIFSLEVHEKEIDKKAEVVRKSY
                     LTLNEYINGG"
     gene            690..1424
                     /locus_tag="VHD02_001837"
     CDS             690..1424
                     /locus_tag="VHD02_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEQ5498414.1"
                     /translation="MNLSIFGLKNIPYLKEGDSIEKLIEESIKTSGFFIEDNDVLCIA
                     SKVVSVAEGQVMSLNEIQVSDVAKEIHRNIPRKDPRIIEIMLNLVNRDLSRLDIKKNY
                     IGCRLENGLKLTSGGIDRKSVDEVFLLPNNPDASAKRISEYLKKSLGKNVAVVITDSD
                     GREDKRGATQVAIGIYGIHPLRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGT
                     GIPAVLIRGLDYKWSENTSIKEALNE"
     gene            1417..2403
                     /locus_tag="VHD02_001838"
     CDS             1417..2403
                     /locus_tag="VHD02_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEQ5498415.1"
                     /translation="MSRNQDCKNILIIGFGKIGKIKAYKWLSRGYEVYVKDLKFSSMK
                     YLNTNSDVLDDLSRQYFTIEICTPTNHHLTLLKTVITRYVYSYISIEKPLCNRLEDLE
                     EMKSLVESHPHLAKKMFASEQYFFSKILEKLLQVDCFSLDKIKEITVNFSKDRIEDNE
                     NGRFLDKEILGYGIEIPHIIAVISYLGISLEEFKKGIFVNAIYIKDLEKHDYSIEILS
                     CINQTVIKIISNLGSFAIREGKIEGGDAKTVRRVTIDDKTILFDPHPKLERYKSELVT
                     GTNTFQIHDDMMDTYISLLEGNAIPEDCYINNAIEITKLLINLYSECEKIYL"
     gene            2413..>2950
                     /locus_tag="VHD02_001839"
     CDS             2413..>2950
                     /locus_tag="VHD02_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase"
                     /protein_id="HEQ5498416.1"
                     /translation="MDLKITNGFYDPSHLSYEVVERKGLGHPDTLADGIAEQIEIDYS
                     LYCLDKFGVIPHHNFDKIIIRGGHSVQDFGGSDFIEPIKIIFLGRASKKCFNSSIPLF
                     KIQKKAATKYLNRILPNLDVENYVEFETLTSDFTTKTNWFSPEAIEDLPEYLDVPKAN
                     DTATMISYWPLTISEELAL"
BASE COUNT         1109 a          350 c          558 g          933 t
ORIGIN      
        1 taatttaaaa gaacggttac aaaagtataa tatcactcca atttcaattg caagcccact
       61 ttttaaatgg aatatagaag aaaatcattt agagagtaat cttgatttat acggtatttc
      121 agctaatgtt acgttaaatg aacaatggct ttatgtaagg aaaattattt ttattgcaaa
      181 atttttaaat atcaaatatg taagaatatt tggtgggatt gggtgctcag tagatgactt
      241 cttgaataat gaactatttt tgaatttgtt aaaagagact gaaattatct ttttaattga
      301 gaacgagtta ggaacttcag tgagcactgc agacgatttg ttaaaaatag gacattttat
      361 agatgaaaaa tctataaaaa atctaaaaat ctggtttgat attgcaaatt attacatagt
      421 tgaacctcag gtagacaagg ttattccaaa attgaaaaaa tatatttact atattcactg
      481 taagaactat gttaacaatg atttaggaat acattatgta gaactaggta aaggaattat
      541 cgattataaa acaatcattc atgaaatcca tgaacatttt atagacttaa tttttagttt
      601 agaagtacat gaaaaagaaa tagacaaaaa ggcagaagta gtgaggaagt cttatctgac
      661 actaaatgag tatattaatg gaggctaaaa tgaatttgag tatatttggt ttaaagaata
      721 ttccttattt gaaagaaggg gatagtatag aaaagcttat tgaagaaagc attaaaactt
      781 caggattttt tatcgaagac aatgatgtgc tttgcatagc ttctaaagtt gtatctgtcg
      841 cagaaggcca ggtgatgtct ttaaacgaga tacaagtgag tgatgtagca aaagaaatcc
      901 acaggaatat tcctcgtaaa gatcctagaa taattgaaat tatgctaaat ttagtcaata
      961 gagatctgtc acgtttagat atcaaaaaaa attacattgg ttgtcgcttg gaaaatggtt
     1021 taaagttgac aagtggtgga atcgatagaa aatcagtaga tgaagtattt cttttaccta
     1081 ataatccgga tgcttctgca aaaagaattt cggaatatct taaaaaatca cttggaaaaa
     1141 atgttgcagt tgttataaca gatagtgatg gtagagagga taaaagaggt gctactcaag
     1201 tagcaattgg tatttatggt attcatcctt tgagaaaaac agaagtaata gattcacaag
     1261 gagagacaat aaaattccaa gaagagactt tgtgtgatat gattgcagct tgtgctggct
     1321 tagtaatggg gcaacgtgga actggaattc cagctgtcct cattaggggc ttggattata
     1381 aatggtctga aaatactagt attaaggaag cgttaaatga gtagaaatca ggactgtaag
     1441 aatattctaa tcattggttt tggaaaaatt gggaaaatta aagcgtacaa gtggctatca
     1501 cgcggatatg aggtatatgt caaagatttg aaattctcca gcatgaaata ccttaataca
     1561 aatagtgatg tgttggatga tttatcacga caatatttta ctattgagat ttgtactccg
     1621 acaaatcacc accttacgct attgaaaacc gttataacaa ggtatgtcta ttcttatatt
     1681 agcattgaaa aaccactgtg taacaggtta gaagatttag aagaaatgaa gtcgctagta
     1741 gaaagtcacc cacatctagc aaaaaaaatg tttgccagtg aacaatactt cttttcgaaa
     1801 attttggaaa aactgttaca agtagattgt ttctctctag ataaaatcaa ggaaataaca
     1861 gttaattttt ctaaagatag gattgaggat aacgagaatg ggagattttt agataaagag
     1921 atattgggct atggaataga gattcctcat atcattgctg tcatttctta tttaggaata
     1981 tcattagaag aattcaaaaa aggaatcttt gtgaatgcta tttatataaa agacttagag
     2041 aaacacgatt attcaataga aatattatct tgtattaatc aaacagtgat taaaattatt
     2101 agtaatttgg gaagttttgc aataagagag ggcaagattg aaggaggtga tgctaaaaca
     2161 gtaagaagag ttaccattga tgacaaaaca atactatttg atccacatcc taaactcgag
     2221 aggtataaga gtgaattggt gactgggaca aatacgtttc aaatacacga tgatatgatg
     2281 gacacgtata tcagtttgtt agaaggaaat gctattcctg aagattgtta tatcaataat
     2341 gcaattgaga taacaaaatt attaattaac ttatattcag aatgtgaaaa aatttattta
     2401 tgaggtgtaa ctatggattt aaaaattaca aacggctttt atgacccaag tcatttatca
     2461 tacgaagtag tagaacgtaa aggattaggg catcctgata ccttagcaga tggaattgct
     2521 gagcaaattg aaattgatta ctcactttat tgtttggaca agtttggtgt tattccacat
     2581 cataattttg ataagattat tatccgaggg ggacattcag ttcaggattt tggtggaagt
     2641 gactttattg aaccaattaa aattattttt ctaggtaggg cgagcaaaaa atgttttaat
     2701 agttcaattc cactttttaa aattcagaaa aaggctgcaa ctaaatattt aaatagaatt
     2761 ttacctaatt tagatgttga aaattatgta gaatttgaga cattgacctc agattttact
     2821 acaaaaacta attggttctc gcctgaagca atagaagatt taccggagta tctagatgta
     2881 ccaaaggcaa atgatacagc tacaatgata agttattggc cattaacaat ttctgaagaa
     2941 ttagcattaa
//
LOCUS       DAUDYX010000092         2906 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.093, whole genome shotgun sequence.
ACCESSION   DAUDYX010000092 DAUDYX010000000
VERSION     DAUDYX010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.093"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(144..1454)
                     /locus_tag="VHD02_001840"
     CDS             complement(144..1454)
                     /locus_tag="VHD02_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEQ5498417.1"
                     /translation="MIHLIIISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KALLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPDALYMILYLIAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHSLIGAQAVNWGASGGANLITSGSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYIRYSKGRTNSSLTIDQPPILS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRQTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWAIIIMLCGMSLLMSLAVKSGLVTLIGHLITTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPTLFPIIATISAQSPHIDIRLLTTATIIGALSTNISPFSSAGSL
                     IQLSLPHIEERSLAFKKQILLGVPISLSLALLTIWILMLLASLS"
     gene            1678..2550
                     /locus_tag="VHD02_001841"
     CDS             1678..2550
                     /locus_tag="VHD02_001841"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEQ5498418.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDRIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGV
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYISSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          883 a          509 c          675 g          839 t
ORIGIN      
        1 ctcttcctca aggatgagtt ttacagcaga gagtttgaag gctttatcaa aggtttttct
       61 tggcataatg acttttcctc gtttccactt aattttgtgt ctactttatt ataccaagtc
      121 caaccttaag ttagacaata aatctaactt aaggaagcta gcagcatgag aatccagatg
      181 gtcaagagtg ccaaacttaa gctgatgggc acacccagaa gaatttgttt tttgaaagca
      241 agactgcgtt cctctatatg gggaagggag agttgaatga gggaaccagc tgatgaaaaa
      301 ggagagatat tagtagatag agcgccaata atagtggctg ttgtgagtaa gcgaatatca
      361 atgtgaggac tttgagcact gatggtagca atgatgggaa agagagttgg ggctacaacg
      421 gatagggtgg aactaaagag tgacatcact ccggctatca cacaaaagaa cagaggtaac
      481 cagaaatgag gaatggttgt tgttatgagg tgccctatca gtgtaactaa acctgacttg
      541 accgctagag acattagtaa gctcatgccg cagagcatga taattatagc ccagggaacc
      601 ttagccaaaa tggcttcttg cttccctaat ttgagcctta aggcgaggca gaccatgagt
      661 attgagacaa agccaatatc aaatgtttgt cgataagtag ctatccatgc gatgtttggg
      721 aaaatgagat gcaacaaggg aaaaagccaa accaaaacca tgctgctgat catgagcaag
      781 gtggtttgtc tttggacctt gctgaggatt ggtggttggt caatagtcaa ggatgagttt
      841 gttcttccct tactgtagcg gatgtaacaa gataataaaa gcaagacgat gagtgggtag
      901 ataatgctga cgataaagat atgattgcca agtgaaaaag cttgctcttc ccatcccatt
      961 tgcttaaaca ggccttgaaa gacaatgcct gagccactgg ttatcaaatt agcccctcct
     1021 gaagctcccc aattgacggc ttgagctcca atcaaagagt gtttgtccgc tttttgacag
     1081 agggtaatcg ctagaggaca gcaaacggcc atagtagtga aaaatccagc acctaaagca
     1141 gacaaaaggg ttgctatcag gtataaaatc atgtagaggg cgtcagggtg ggtgcgtgtg
     1201 cggtagagaa tgtgttgagc caaaacatca agagtgccgt tagttgttgc aacgttataa
     1261 aagagagaga cgctaaaaat ggtaaaaaag agtgaggttg gccaaaaatg aagaagtgct
     1321 ttggggctta atcccatgag agtggttgcg atgaggtaag aaaaagcaat agccagcagg
     1381 ccaatattga ttttggtgcg gtaaccaatt ccaatggcta gagcaatggc gctaattatt
     1441 attaaatgaa tcattgaatt gtcctttagt tagaatatga aaataggttg gcaattttgg
     1501 cgctcaaaag accacctgtt tcctattggt acttgactat tatacacaaa aaaattacaa
     1561 ttgtcttgac acttgttggg atttaaaatg aggtattcag gtttgtgatt tcctacggaa
     1621 gttaatagca attgcctcta gtgcttacaa gtgatattaa aaatagagga cttagtgatg
     1681 tcaatcattt caactgattt aacccctttt caaatagatg atacattgaa agcagccttg
     1741 cgagaagatg ttcattccga agattacagt accaatgcca tttttgatca tcatggccaa
     1801 gccaaggtgt cgctttttgc caaggaagct ggtgttttag cggggctaac cgtttttcaa
     1861 agggttttta ccctatttga tgccgaggtg accttccaga atcctcatca atttaaggat
     1921 ggggatcgtt tgactagtgg cgatttggtt ttagaaatca taggctcggt gagaagcctc
     1981 ttaacatgtg aacgcgttgc cttgaatttt ttacaacatt tatcagggat tgcttcgatg
     2041 acagctgctt atgtagaagc cttaggcgat gatcgcatta aggtatttga tactcgaaaa
     2101 accactccta atttacgtct ttttgagaaa tatgccgtga gagttggcgg tggctataat
     2161 catcgcttta atttatcaga tgctatcatg ctaaaagaca atcacattgc ggcagtaggt
     2221 agtgttcaaa aggcgattgc tcaagcgcgt gcctatgccc cttttgtgaa aatggtcgag
     2281 gtggaagtgg aaagccttgc tgctgccgaa gaagctgcgg cggcgggtgt tgatattatc
     2341 atgttggata atatgtcatt ggaacagatt gaacaggcca ttactctaat tgcaggacgt
     2401 tctcggattg aatgttctgg aaatattgat atgaccacta ttagccgttt tcgtggttta
     2461 gcgattgatt acatctccag tggtagttta acccatagtg ctaagagtct cgatttttcc
     2521 atgaagggtt taacctacct tgatgtctaa gttgcaaaat aaactaactt tttaaagaat
     2581 gcctttcctc tagaacgagt tttatgtcag atagtttaaa cgcctcttca aatactttcc
     2641 ctgttttcac ctgattcgtg tccgcttatt ataccaattc caagcattca cctgaaaaaa
     2701 cgatagggag aggttagtgt ttttattgta aaagttccac gccaaatcca tgaagaaaag
     2761 tttgttttca gaaatcacga ttcatcataa taaaatcaat caaagtcgtt aactaggagt
     2821 gtactgaccc caaaaagttg gacaaataat tattgaaagg atttagttct gtattggaca
     2881 ggactaaatc cttttagttt tgcttt
//
LOCUS       DAUDYX010000093         2650 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.094, whole genome shotgun sequence.
ACCESSION   DAUDYX010000093 DAUDYX010000000
VERSION     DAUDYX010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.094"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(17..627)
                     /locus_tag="VHD02_001842"
                     /pseudo
     CDS             complement(17..627)
                     /locus_tag="VHD02_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            946..1173
                     /locus_tag="VHD02_001843"
     CDS             946..1173
                     /locus_tag="VHD02_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEQ5498419.1"
                     /translation="MEVKKLETFHQMTIEKLAKVEGGKNNWQANVSGVIAAGSAGAAI
                     GFPVCGVACGYIGAKTAITLWAGVTGATGGF"
     gene            1186..1386
                     /locus_tag="VHD02_001844"
     CDS             1186..1386
                     /locus_tag="VHD02_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="HEQ5498420.1"
                     /translation="MTTMKELTINDMASISGGNAPGDAVIGGLGGLASGLKFCKLLHP
                     VLAGGCVVGFTVGGAYLGYTAN"
     gene            1680..1874
                     /locus_tag="VHD02_001845"
     CDS             1680..1874
                     /locus_tag="VHD02_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEQ5498421.1"
                     /translation="MEVLKNGLAIINGGGKDGANIFLTGMAGAAQGVTVCAQTGVFIP
                     WQGYILCGAAGAATNIIWPH"
     gene            2399..>2650
                     /locus_tag="VHD02_001846"
     CDS             2399..>2650
                     /locus_tag="VHD02_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498422.1"
                     /translation="MRNILRFLGIVIISAVILFSIGSFYDLPLMKNIQLICWSFILDL
                     FVFVYKQRQTTEVLTWYQVVKQFWLFIKCTILIPTLIAFI"
BASE COUNT          841 a          374 c          530 g          905 t
ORIGIN      
        1 atgtcttgta agtattttag tcttcgaaaa tacttacaag acatcgttct aaggcttctt
       61 catttgggaa aaccactctc ttcttgtttt gtcgcttaat ttctttattg agtgattcaa
      121 tcaagtttgt agagtaaatg ctagaccaaa tgtgatgtgg gaattgatag aaagtaagga
      181 gattttccgt cgtttcaagt gatgccatga cctttttata acgaggcttc cactcagaaa
      241 caagtggtga ccatacatgc gctcaataat gtcactgatt tcgcgtgtcg taacgccagt
      301 ctgatagagt ttgatcacca tctcttccaa gtggtcatct cgtctagcat aagatggtaa
      361 taaaactggt gaaaactcgc cgtttcgatc tcttgggatg attaaattga ctaagccata
      421 ctttgtttca aactgacgag aataagtccc attacggcta ttacctgtgt tgtaaccttc
      481 tttttcataa ggttcataac caagaaaagc tgatagttca acttgcaaga ggtcattcat
      541 agctatttct aaagaggagc gaaagaattc atcaatatct tgtttttgtg ctaggaagtt
      601 aagtaattct gtggtaaact gagtcatggg aatgaatctc tttctagtga agtgtcgtaa
      661 ctctactata caagatttat tcctttttgt gtgtttacac aagatatttt acactaccat
      721 caaaaatctt aaaaggtatt tataaaagca tgaatggtat tatttaaact tcttaattaa
      781 ttaactattg tcacagcgta agtgtagaga ggataaaaag acatttcatg atgaaaaaca
      841 gccttttaag attttgaaac catgatactg ttctttatga tattctgtac tagctcgagg
      901 ttgcaacgag acaaatttaa gaattatgaa aagaagttat tcgatatgga ggtaaagaaa
      961 ctggaaacat ttcaccaaat gactatagaa aagcttgcca aggttgaggg tggtaagaat
     1021 aattggcagg caaatgtcag tggggttatc gctgcaggta gtgctggagc tgctataggt
     1081 tttccagttt gcggtgtagc ctgtggttat attggagcta aaacagctat tactctttgg
     1141 gctggtgtaa caggagctac aggcggattc tagaggaaag aaaggatgac aacaatgaag
     1201 gaattaacaa ttaatgacat ggctagtata agtggaggaa acgctccagg agatgctgta
     1261 attggtggtc ttggaggctt agcttctgga ttgaaatttt gtaaactgct acatccagta
     1321 ttggcaggtg gatgtgttgt aggatttaca gttggtggag catatttggg atatacagca
     1381 aattaaatga tctatacgat ttttattatt atgaatgcgt ctataatgtt tttaatcatt
     1441 gttgatgcca ttcataaaaa aatgttgata aatctatacc atatttagtg tttttaattt
     1501 tcaatcaatt atccctttca gaaaaatcgg taaacatgtt atctatcgta gctttggtag
     1561 cgtcattatt tgtttttatc gataaaagaa aatagtgatt atgccaatga atttagatat
     1621 cactcttagc tttttaaaat acaaaagagt gaaattaaaa tgaaaaagac ttcaaaaata
     1681 tggaagtgct aaaaaatggt ctggctatca taaatggagg cggaaaagac ggagcaaata
     1741 tctttttgac agggatggca ggtgctgcgc agggagtgac agtttgtgct cagacaggcg
     1801 tatttatccc ttggcaaggt tatattttgt gtggagcagc tggtgctgct acaaatatta
     1861 tctggcctca ctaaatatat aaatgactat ctatcactga tgtataagta gtagaggagt
     1921 ggtatgaaaa ctttagatat tatttttaat ctatctgata gaaagatgat atttctagaa
     1981 aatgttgaaa agctgggagg ttacattgtc ccttttaccc ttatcatctt gagtgtagcc
     2041 tacaacttta ggcaccctta tcctatttgg gctgctgtac taggaggtat atcccttatt
     2101 gtgatcttta tttattttat acttattgtt tatctatatt tgcgttatag atacttggtg
     2161 tggaaaaaaa taccagaaat taagattaat tctactgagc tatcatgcaa tcacatttaa
     2221 ggggaaaatg gatgacttat acaatatcag tacttatgat atttaacttt agagactaaa
     2281 gagttaaaag caaaatgcgt attgaaaatt ggttaaaaaa gttataactg tctctgataa
     2341 caggaataat aatttgttcg gttatatttg ctaccgatag agtagaggag gtgaagatat
     2401 gcgtaatata ttaagatttt taggtattgt tattatttct gcagtgattc tatttagtat
     2461 aggtagtttc tacgatttac cattaatgaa aaatatacaa cttatctgtt ggagttttat
     2521 ccttgattta ttcgtttttg tctacaaaca aagacaaacg acagaagtgc tcacatggta
     2581 tcaagttgtt aagcaatttt ggttatttat taagtgtaca attcttattc ctaccttaat
     2641 tgcttttatt
//
LOCUS       DAUDYX010000094         2586 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.095, whole genome shotgun sequence.
ACCESSION   DAUDYX010000094 DAUDYX010000000
VERSION     DAUDYX010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.095"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(<1..431)
                     /locus_tag="VHD02_001847"
     CDS             complement(<1..431)
                     /locus_tag="VHD02_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEQ5498423.1"
                     /translation="MLTTNQIHKLLGVKEVYKAPDTLIKIILDKEKREDLFRQFLKYE
                     TDVSYDWFMQYFEEEQADRKNKKQDFTPKSVSTLLSKIISGNQYYEVAVGTGGILIQA
                     WQEQRLNDSPFTYRPSKYWYHVEELSDKAVPFLLFNMSIRG"
     gene            complement(431..832)
                     /locus_tag="VHD02_001848"
     CDS             complement(431..832)
                     /locus_tag="VHD02_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498424.1"
                     /translation="MKNENFLGYDLAELLEEKLVGKRLKSIDDEKIVLADGTVIKIEL
                     NEGCGGCGNGWSELNITTENPNLESAVMGVEYTEKYSEWDDEFKIFVYMTDNSVIEIY
                     GYDGVGNGYYGYGFWVTVKNPGDLIESELED"
     gene            complement(1023..1358)
                     /locus_tag="VHD02_001849"
     CDS             complement(1023..1358)
                     /locus_tag="VHD02_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498425.1"
                     /translation="MDYLMIGIIVLSVVIFIMGFFILYYQAIIVNSMKNDFRRMRKEL
                     REELGFDSYNWSDSFRNMRRDIKSNKELISEINSLQIIKKAKEAKRLEELEQTKANVE
                     KEINKLNGG"
     gene            complement(1361..1633)
                     /locus_tag="VHD02_001850"
     CDS             complement(1361..1633)
                     /locus_tag="VHD02_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498426.1"
                     /translation="MTEEQELIKPLLEEIERKDDRLYMQSNVIAVLMTAVLMLLMISI
                     ALQNHYEPQITGLRTQLSRTQKQLKRASEDRTRQTKRIAELTGNGG"
     gene            complement(1636..1857)
                     /locus_tag="VHD02_001851"
     CDS             complement(1636..1857)
                     /locus_tag="VHD02_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498427.1"
                     /translation="MKIDYINFFEQVVPDWMRESNVKMQEVGFNTEAYWQWANQSIVA
                     ICEKYGNDSLINGQFHLIWEWLEDKANGG"
     gene            complement(1854..2249)
                     /locus_tag="VHD02_001852"
     CDS             complement(1854..2249)
                     /locus_tag="VHD02_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEQ5498428.1"
                     /translation="MIEFFLPMDKIPTTTHQQKKVTVINGKPHFYEPESLKNARDKFT
                     SLLAQHVPPSKLDGPIRLTVKWLFPKIKGSTNGQYKTTKPDTDNLQKLLKDCMTELGF
                     WNDDAQVASEIIEKFWADTVGIYVKVEEL"
     gene            complement(2242..2460)
                     /locus_tag="VHD02_001853"
     CDS             complement(2242..2460)
                     /locus_tag="VHD02_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498429.1"
                     /translation="MEIYKQRMIEEYKQLKKRAEKLSIVLNRYYLDELDFELSCPIEL
                     LQTQWHIMGAYLKILEQRFLVEGIYFND"
BASE COUNT          708 a          568 c          371 g          939 t
ORIGIN      
        1 atccctctta tagacatatt aaagagtaga aacggtactg ctttgtccga taactcttct
       61 acgtgatacc agtatttact cggacgataa gtaaatggac tgtcatttaa tcgttgttct
      121 tgccatgctt ggataagtat cccacccgtc ccgactgcta cctcgtagta ttgattacca
      181 cttattattt tagataatag tgtactaacc gatttaggcg taaagtcttg ctttttattt
      241 ttgcggtcag cttgttcttc ctcaaaatat tgcataaacc agtcgtaaga tacatctgtt
      301 tcatatttca aaaactgtct gaataagtcc tcgcgttttt ccttgtctaa aattattttt
      361 atcaacgtgt caggcgcttt gtagacttct tttactccca gcagtttgtg tatttgattt
      421 gtagttagca ttagtcctcc aattcagatt ctattagatc accaggattc ttcacagtaa
      481 cccagaaacc atatccataa tatccattac caacgccgtc atatccgtaa atctcaataa
      541 cagaattgtc tgtcatgtag acaaaaattt tgaattcatc atcccattca ctatattttt
      601 ctgtgtattc aacacccatt acagcagatt ccaagtttgg gttttctgtc gtgatgttta
      661 attctgacca cccatttcca caacctccac aaccctcgtt taattcaatt ttaattactg
      721 tcccatcggc aagtacaatc ttttcgtcgt ctattgattt aagtcgtttc cctactaatt
      781 tttcttctaa tagttcagct agatcataac ctaaaaaatt ttcgtttttc attttgtacc
      841 tacctcactt attttttgtt ttatctatcg tcttgctaaa agtaaacgaa acgatcccgg
      901 cggctatata atacagtatg caagatatta caattagaat agtcgcaaaa acacttaata
      961 taacaacagc aactaaaatt ttagctaaac agcttattaa agctaaacat ccagatacca
     1021 cttcatcccc cattcaactt attgatttct ttttcgacgt ttgcttttgt ctgctcgagt
     1081 tcttctagtc gtttagcttc tttggctttt tttatgattt gcaaagagtt tatttcggat
     1141 attaactctt tatttgattt aatatctcta cgcatatttc tgaaactgtc agaccagtta
     1201 tagctatcaa aaccgagttc ttctctcaat tctttacgca ttctacgaaa atcatttttc
     1261 atcgaattaa caattattgc ttgatagtaa agtatgaaaa atcccattat aaaaattact
     1321 actgataata ctattattcc aatcattaaa taatccatcc ttatccccca tttcctgtca
     1381 attccgcaat ccgcttagtc tgtctggtcc tatcttcgct agcacgttta agctgctttt
     1441 gtgtcctact tagctgtgta cgtagtcctg tgatttgcgg ctcgtagtgg ttttgtaagg
     1501 ctatacttat catcagtaac ataagaactg ctgtcattaa gacggcaatt acattagact
     1561 gcatatacaa tctatcatct ttccgctcta tctcttctag cagcggtttt attagttctt
     1621 gctcttcagt catcgttaac ctccgtttgc tttatcctcc aaccattccc aaatcagatg
     1681 aaactgccca ttgatcagac tgtcatttcc gtacttttcg cagattgcaa caatagattg
     1741 atttgcccat tgccaatacg cttcagtatt aaatccaact tcttgcattt tgacattact
     1801 ctctctcatc cagtcaggaa ccacttgttc aaaaaaatta atataatcaa ttttcataac
     1861 tcttccacct ttacgtatat tcctactgta tcagcccaga acttctcaat aatttccgaa
     1921 gcaacctgcg catcatcatt ccaaaatccc aattcggtca tgcagtcttt aagcagcttc
     1981 tgcaggttgt cggtatctgg ttttgtcgtt ttgtactgtc cgtttgtgct gcccttgatt
     2041 ttgggaaata gccacttgac tgtcagacgg ataggaccat ctaacttact gggtggtaca
     2101 tgctgagcca aaagacttgt aaacttatcc ctagcatttt tcaaactctc aggctcgtaa
     2161 aagtgaggct tgccatttat gacagtcact ttcttttgct gatgagttgt cgtcgggatt
     2221 ttatccatag gcaaaaaaaa ttcaatcatt aaaataaatc ccttcaacca aaaatctttg
     2281 ttctagaatt ttcaaatacg ctcccataat atgccattgc gtttgtaaaa gttcaattgg
     2341 gcaagataat tcaaaatcta actcatcaag gtagtatctg tttagcacaa tagatagttt
     2401 ttctgcgcgt ttttttaact gtttatattc ttcaatcatt ctttgtttgt aaatttccat
     2461 ttttacctca tctccaattt gtgtttttta tatgcgcctt tgtcaaatat gggacaaagg
     2521 acagaggggg cgtagctcag tcgcccctct tccttttccc tttgactttt gacgaaggaa
     2581 aaacat
//
LOCUS       DAUDYX010000095         2278 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.096, whole genome shotgun sequence.
ACCESSION   DAUDYX010000095 DAUDYX010000000
VERSION     DAUDYX010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.096"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..606
                     /locus_tag="VHD02_001854"
     CDS             <1..606
                     /locus_tag="VHD02_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498430.1"
                     /translation="EELYLSIGTGIYHFQAEAIDIYSQFEESLSDYTFRYFCEEFSDN
                     FWKFIDSMDSEVNKENLTATSLEILKKNSAILVNAEFNESRFLSDNSLFFKSVLDKIK
                     HLYYYTMHHTGQRHHNTNTFDYSKFNSYIPYINYIFYRISSNPGLKEDEKVIDFVKRC
                     NNLISSDYSAESWYSERQMQDVIKKINEKYTILEDSGRESD"
     gene            975..2267
                     /gene="purB"
                     /locus_tag="VHD02_001855"
     CDS             975..2267
                     /gene="purB"
                     /locus_tag="VHD02_001855"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEQ5498431.1"
                     /translation="MIERYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VAKIREKADFDIDRILEIEQDTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAREHKMTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEQYVCYKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDIKVTSCLTQEEIDELFNPIYYTKRVDDIFKRLGI"
BASE COUNT          723 a          388 c          493 g          674 t
ORIGIN      
        1 gaggaattgt atctgtctat tggaacgggt atttatcatt tccaagctga agctattgat
       61 atttattctc aatttgaaga gtcgctgagt gattatacat ttagatattt ttgtgaagaa
      121 ttttcggata atttttggaa gtttattgat tctatggatt ccgaagttaa taaagagaac
      181 ttgacagcta ctagtttaga aattttgaag aaaaattctg ctattttagt aaatgcagaa
      241 tttaatgaga gccgattttt atctgataat agtctttttt tcaaatctgt gttagataaa
      301 attaagcatt tatattacta tactatgcat catacgggtc aacgtcatca taatacaaat
      361 acttttgact attcaaaatt taattcatat attccttata ttaactatat attttatagg
      421 atatcatcaa atcctggatt aaaagaagat gaaaaagtaa tagattttgt taagaggtgt
      481 aataatttaa tttcaagtga ctatagtgca gaatcttggt atagtgagcg tcaaatgcag
      541 gatgtaatta aaaaaattaa tgaaaaatac actatattgg aagatagtgg aagggagtcg
      601 gattaatcaa attaaatttg gtagagagtt taggaaatct aacttactca ttctaaatgc
      661 taaggaaatt aagttaaaaa atagaaagaa acaaaggtag tcgtattcgc taaactgaat
      721 acaggctacg gacttcctta actcacagaa ttttaagaaa tacaagaaag gagtgagggt
      781 gtttccgagt atttgaactc gggctaactt atctcctcaa tacaaatcaa ataatagaaa
      841 ggaaatgggc actagtttca ctaaactgaa cccgagcgga aagctcggaa tttagataaa
      901 ccgcctagga cgcaagcgtc cattgttggt ttcctaaaat tcagtcgctt tctggtcgcc
      961 cttggtatct taaaatgatc gaacgttatt cacgccctga gatggcggca atttggacag
     1021 aggaaaataa ataccgtgct tggttggagg ttgagatttt ggctgacgag gcatgggctg
     1081 agttgggtga gattcctaag gaggatgtgg ctaagattcg tgagaaggcg gattttgaca
     1141 ttgaccgcat tcttgagatt gagcaggaca cacgtcacga tgtggtggct ttcactcgtg
     1201 cggtttctga aacgcttggt gaggagcgca agtgggtgca ctacggtttg acgtcaactg
     1261 acgtggtgga cactgcctac ggttacctct acaagcaagc taacgacatt atccgtcgcg
     1321 atcttgagaa tttcaccaat atcgtggcag acaaggcgcg tgagcacaaa atgaccatca
     1381 tgatgggtcg cacccacggt gttcacgccg agccaacgac tttcggtctt aagttggcga
     1441 cttggtacag cgagatgaaa cgtaatattg agcgttttga acatgctgcc gcaggtgtgg
     1501 aagctggtaa gatttcaggt gccgttggta actttgctaa tatccctcca tttgtggaac
     1561 aatatgtctg ttacaaatta ggcattcgtc cacaagagat ttcaacacaa gttcttccac
     1621 gtgacctcca cgcagaatat tttgcagtgc ttgcaagtat tgcaacttct atcgaacgta
     1681 tggcgacaga gattcgaggt ctgcaaaagt cagaacaacg tgaagttgaa gaattctttg
     1741 ccaaaggtca gaaaggtagc tctgctatgc ctcacaaacg caacccaatc ggttcagaga
     1801 acatgacagg gctagcgcgc gtgattcgtg gtcacatggt gacagcttat gagaacgtgt
     1861 cactttggca tgagcgtgat atttcacact catcagctga gcgtatcatc acacctgaca
     1921 caactatctt gattgactac atgctcaacc gctttggcaa tatcgttaag aacttgactg
     1981 tcttcccgga aaatatgatg cgcaatatgg aatcaacttt tggtttgatt tatagtcaac
     2041 gtgttatgct caaattgatt gaaaaaggaa tgacacgaga agaagcttat gacttagttc
     2101 aacctaagac agcttattcc tgggacaatc aagtggattt caaaccactt ttagaagaag
     2161 acatcaaagt tacctcttgt cttacacaag aagaaattga tgaactattt aatccgattt
     2221 attacacaaa acgtgttgat gatattttta agcgtttagg gatttaatat aaaaaaaa
//
LOCUS       DAUDYX010000096         2274 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.097, whole genome shotgun sequence.
ACCESSION   DAUDYX010000096 DAUDYX010000000
VERSION     DAUDYX010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.097"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..1123
                     /gene="hasA"
                     /locus_tag="VHD02_001856"
     CDS             <1..1123
                     /gene="hasA"
                     /locus_tag="VHD02_001856"
                     /EC_number="2.4.1.212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hyaluronan synthase HasA"
                     /protein_id="HEQ5498432.1"
                     /translation="KLGLSFLYEPFKGNPHDYKVAAVIPSYNEDAESLLETLKSVLAQ
                     TYPLSEIYIVDDGSSNTDAIQLIEEYVNREVDICRNVIVHRSLVNKGKRHAQAWAFER
                     SDADVFLTVDSDTYIYPNALEELLKSFNDKTVYAATGHLNARNRQTNLLTRLTDIRYD
                     NAFGVERAAQSLTGNILVCSGPLSIYRREVIIPNLERYKNQTFLGLPVSIGDDRCLTN
                     YAIDLGRTVYQSTARCDTDVPFQLKSYLKQQNRWNKSFFRESIISVKKILSNPIVALW
                     TIFEVVMFMMLIVAIGNLLFNQAIQLDLIKLFAFLSIIFIVALCRNVHYMVKHPASFL
                     LSPLYGILHLFVLQPLKLYSLCTIKNTEWGTRKKVTIFK"
     gene            1159..>2274
                     /gene="hasB"
                     /locus_tag="VHD02_001857"
     CDS             1159..>2274
                     /gene="hasB"
                     /locus_tag="VHD02_001857"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase HasB"
                     /protein_id="HEQ5498433.1"
                     /translation="MKIAVAGSGYVGLSLGVLLSLQNEVTIVDILPSKVDKINNGLSP
                     IQDEYIEYYLKSKQLSIKATLDSKAAYKEAELVIIATPTNYNSRINYFDTQHVETVIK
                     EVLSVNSHATLIIKSTIPIGFITEMRQKFQTDRIIFSPEFLRESKALYDNLYPSRIIV
                     SCEENDSPKVKADAEKFALLLKSAAKKNNVPVLIMGASEAEAVKLFANTYLALRVAYF
                     NELDTYAESRKLNSHMIIQGISHDDRIGMHYNNPSFGYGGYCLPKDTKQLLANYNNIP
                     QTLIEAIVSSNNVRKSYIAKQIINVLEERESPVKVVGVYRLIMKSNSDNFRESAIKDV
                     IDILKSKDIKIIIYEPMLNKLESEDQSVLVNDLENFKK"
BASE COUNT          781 a          363 c          383 g          747 t
ORIGIN      
        1 taaacttgga ttatctttcc tttatgagcc atttaaagga aatccacatg actataaagt
       61 tgctgccgta attccttctt ataatgaaga tgccgagtca ttattagaaa ctcttaaaag
      121 tgtgttagca cagacctatc cgttatcaga aatttatatt gttgatgatg ggagttcaaa
      181 cacagatgca atacaattaa ttgaagagta tgtaaataga gaagtggata tttgtcgaaa
      241 cgttatcgtt caccgttccc ttgtcaataa aggaaaacgc catgctcaag cgtgggcatt
      301 tgaaagatct gacgctgacg tttttttaac cgtagattca gatacttata tctatccaaa
      361 tgccttagaa gaactcctaa aaagcttcaa tgataagaca gtttatgctg caacaggaca
      421 tttgaatgct agaaacagac aaactaatct attaacgcga cttacagata tccgttacga
      481 taatgccttt ggggtggagc gtgctgctca atcattaaca ggtaatattt tagtttgctc
      541 aggaccattg agtatttatc gacgtgaagt gattattcct aacttagagc gctataaaaa
      601 tcaaacattc ctaggtttac ctgttagcat tggggatgat cgatgtttaa caaattatgc
      661 tattgattta ggacgcactg tctaccaatc aacagctaga tgtgatactg atgtaccttt
      721 ccaattaaaa agttatttaa agcaacaaaa tcgatggaat aaatcttttt ttagagaatc
      781 tattatttct gttaaaaaaa ttctttctaa tcccatcgtt gccttatgga ctattttcga
      841 agtcgttatg tttatgatgt tgattgtcgc aattgggaat cttttgttta atcaagctat
      901 tcaattagac cttattaaac tttttgcctt tttatccatc atctttatcg ttgctttatg
      961 tcgtaatgtt cattatatgg tcaaacatcc tgctagtttt ttgttatctc ctctgtatgg
     1021 aatattacac ttgtttgtct tacagcccct aaaactttat tctttatgca ccattaaaaa
     1081 tacggaatgg ggaacacgta aaaaggtcac tatttttaaa taatatatgc atcgagtagt
     1141 tagagaagga gtaattttat gaaaatagca gttgctggat caggatatgt tggattatca
     1201 ctaggagttc ttttatcact tcaaaacgaa gtcactattg ttgatattct tccctctaaa
     1261 gttgataaga ttaataatgg cttatcacca attcaagatg aatatattga atattactta
     1321 aaaagtaagc aattatctat taaagcaact ttagatagca aagcagctta taaagaagcg
     1381 gaactggtca ttattgccac acctacaaat tacaacagta gaattaatta ttttgataca
     1441 cagcatgttg aaacagttat caaagaggta ctaagcgtta atagccatgc aactcttatc
     1501 atcaaatcaa caattccaat aggtttcatt actgaaatga gacagaaatt ccaaactgat
     1561 cgtattatct tcagccctga atttttaaga gagtctaaag ctttatatga caacttatat
     1621 ccaagccgaa ttattgtttc ttgtgaagaa aacgattctc caaaagtaaa ggcagacgca
     1681 gaaaaatttg cacttttatt aaagtctgca gctaaaaaaa ataatgtacc agtacttatt
     1741 atgggagctt cagaagctga agcagtaaaa ctatttgcca atacttattt agcgttaagg
     1801 gtagcttatt ttaatgagtt agacacttac gcagaatcga gaaaattaaa tagtcacatg
     1861 attattcaag gaatttctca tgatgatcga ataggaatgc attataataa cccatcattt
     1921 ggttatggag gttattgtct acctaaagat acgaagcaat tattggcaaa ttacaataat
     1981 attccccaaa cgctaattga agctatcgtt tcatcaaata atgtgcgcaa gtcctatatt
     2041 gctaagcaaa ttatcaacgt cttagaagag cgggagtccc cagtaaaagt agttggggtt
     2101 taccgtttaa ttatgaaaag taactcagat aattttagag aaagtgctat caaagatgtt
     2161 attgacattc ttaaaagtaa agacattaag ataattattt atgagccaat gttaaacaaa
     2221 cttgaatctg aagatcaatc tgtacttgta aatgatttag agaatttcaa gaaa
//
LOCUS       DAUDYX010000097         2272 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.098, whole genome shotgun sequence.
ACCESSION   DAUDYX010000097 DAUDYX010000000
VERSION     DAUDYX010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.098"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..307
                     /locus_tag="VHD02_001858"
     CDS             <1..307
                     /locus_tag="VHD02_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEQ5498434.1"
                     /translation="FEEEESQKRNRFMGWILVSMMFLFILPTYNLVKSYVDFEKQNQQ
                     VVKLKKEYNELSKRTKKEKQLAERLKDDNFVKKYARAKYYLSREGEMIYPIPGLLPK"
     gene            442..1728
                     /locus_tag="VHD02_001859"
     CDS             442..1728
                     /locus_tag="VHD02_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEQ5498435.1"
                     /translation="MRKLLAAMLMTFFLTPLPVISTEKKLIFSKNAVYQLKQDVVQST
                     QFYNQLPSNPNLYQETCAYKDSDLTLPAGRLGVNQPLLIKSLVLNKESLPVFELADGT
                     YVEANRQLIYDDIVLNQVDIDSYFWTQKKLRLYSAPYVLGTQTIPSSFSFAQKVHATQ
                     MAQTNHGTYYLIDDKGWASQEDLVQFDNRMLKVQEMLLQKYNNPNYSIFVKQLNTQTS
                     AGINADKKMYAASISKLAPLYIVQKQLQKKKLAENKTLTYTKDVNHFYGDYDPLGSGK
                     ISKIADNKDYRVEDLLKAVAQQSDNVATNILGYYLCHQYDKAFRSEIKALSGIDWDME
                     QRLLTSRSAANMMEAIYHQKGQIISYLSNTEFDQQRITKNITVPVAHKIGDAYDYKHD
                     VAIVYGNTPFILSIFTNKSTYEDITAIADDVYGILK"
     gene            1725..>2272
                     /gene="tilS"
                     /locus_tag="VHD02_001860"
     CDS             1725..>2272
                     /gene="tilS"
                     /locus_tag="VHD02_001860"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEQ5498436.1"
                     /translation="MMTYQEIFNEIKNKAYFKNHRHVLIAVSGGVDSMNLLHFLYLFQ
                     DKLKIRIGIAHVNHKQRSESDSEEAYLKCWAKKHDIPIYVSNFEGIFSEKAARDWRYA
                     FFKSIMLKNNYSALVTAHHSDDQAETILMRLIRGSRLRHLSGIKSVQPFANGQLIRPF
                     LTFSKKDLPEIFHFEDSSNRELS"
BASE COUNT          849 a          333 c          368 g          722 t
ORIGIN      
        1 atttgaagaa gaagaatctc aaaaaagaaa tcgttttatg ggatggatcc ttgtaagtat
       61 gatgttttta tttattttgc caacttataa tcttgtcaaa agttatgttg attttgaaaa
      121 gcaaaatcaa caggtggtta aattaaaaaa agagtataat gaattgtcaa agaggacaaa
      181 aaaagaaaaa caattagcag aacgactaaa agatgataat tttgtcaaaa aatatgctag
      241 ggcaaaatac tatttatcgc gtgaaggaga aatgatttat cctattccag gactattacc
      301 aaaatgatga tggacaatat tgtaaaaaaa atagaagcat ttcttgcttt ttctgataaa
      361 aaattagcag agctgcaaca ggaaaatcaa aaagttaaag aagaaagtca gtatattcaa
      421 aaaaactaga aaggagtcaa tatgagaaaa ttattagcgg ctatgttaat gacttttttt
      481 ctgactcctt taccagtgat tagtacagaa aaaaaactta tattttcaaa aaatgctgtt
      541 tatcaattga aacaagatgt cgttcaatca acacaattct ataatcagct accctctaat
      601 ccaaatcttt atcaagaaac gtgtgcctat aaagacagtg atttaactct accagcagga
      661 agattaggtg taaatcaacc attacttatt aaatcgcttg ttcttaacaa agaatcttta
      721 ccggtttttg agttagctga tggtacctat gttgaggcta atcgacaatt gatttatgac
      781 gatattgtac ttaatcaagt agatatagat agctattttt ggacacaaaa gaaacttagg
      841 ctttattcag ccccttatgt tttaggtacg caaacaattc cttcttcttt ttcatttgct
      901 caaaaagttc atgccactca aatggcacaa acaaaccatg gaacttatta tcttattgat
      961 gataagggct gggcatcaca agaagatcta gttcaatttg ataaccgcat gttaaaagtc
     1021 caggaaatgc tcttacaaaa atataataac ccaaattatt caatttttgt aaagcaactc
     1081 aacacacaaa caagtgctgg tattaatgct gataaaaaaa tgtatgctgc aagtatctcg
     1141 aagttagcac cactttatat tgttcaaaaa caattacaaa aaaagaaatt agcagagaat
     1201 aaaactttga cttatactaa agatgttaat catttttatg gagactatga tccattggga
     1261 agtggtaaaa ttagtaaaat agctgataat aaagattatc gtgttgaaga cctactgaaa
     1321 gctgtagcac aacaatcgga taatgtagca actaatattt taggttatta tctatgtcat
     1381 cagtatgata aagctttccg ctcagagata aaagctttat caggtatcga ttgggatatg
     1441 gagcagcgct tattaacttc tcgttcagct gcaaatatga tggaagctat ttatcatcaa
     1501 aaaggccaaa ttatttctta cctttcaaat accgaatttg atcaacaacg tatcacaaaa
     1561 aatattactg ttccagttgc acataaaatt ggtgatgctt atgattataa acatgacgtt
     1621 gctattgttt acggtaatac tccatttatt ttgtctattt ttacaaataa atcaacctat
     1681 gaagatatta cggctattgc agatgacgtt tatggtattt taaaatgatg acctatcaag
     1741 aaatttttaa tgaaataaaa aataaagcct actttaaaaa tcatagacat gtgttaatag
     1801 ccgtatctgg tggagtagat tcaatgaatt tattgcactt tttatacctg tttcaagaca
     1861 aattgaaaat tcgtattggt attgctcatg ttaatcacaa acaaagatca gaatcagatt
     1921 cagaagaagc ttatttaaag tgttgggcta aaaaacatga catacctatt tatgtttcta
     1981 attttgaagg aatattttca gaaaaagcag cacgtgattg gcgctatgct ttttttaaaa
     2041 gtattatgtt aaaaaataac tattcagctt tagtaacagc tcaccattca gatgatcaag
     2101 cagaaactat tttgatgagg ctaattagag gaagccgatt gaggcacttg tcgggtataa
     2161 aaagcgtgca accatttgcc aatggtcaac ttataagacc ttttttaact ttctcaaaaa
     2221 aggacttgcc agaaattttt cattttgaag attcttcaaa tagggaatta tc
//
LOCUS       DAUDYX010000098         1839 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.099, whole genome shotgun sequence.
ACCESSION   DAUDYX010000098 DAUDYX010000000
VERSION     DAUDYX010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.099"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..>1839
                     /locus_tag="VHD02_001861"
     CDS             <1..>1839
                     /locus_tag="VHD02_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEQ5498437.1"
                     /translation="TVNESEEDRNISFSKPIKLLVEKCKTRYIKYEELIDVFKNNYPD
                     IVSDMLESYIHDLVTNDFLISDLRPPICNINSLDYLLSKLEEGTLCTDLTTLKKMIED
                     YNDLNIGEGRDLLLQIFDKMESIYKYDNGGNYLKVDSLAEYEGGFNILSETARESIER
                     LAEMFVKLNTNVKRRGGRKNSLEEYQLKFIGKYGENCSIPFVKVINKDAGIGFPEYYK
                     GGEGEAIELSDPIMQMFEKKYEEALLNGGHIEFYSKDLDDFQTDQSNSLDSFELNFNI
                     KIINNDVKLYLGANIGSGQAGRSFGRFYGLSETVRETIKNLNHQNNTNVELSFVPKQI
                     RLANVIQNYSDESYNTSFFTTSWDSENELRLEDIYIRYSDGKFHFTTIDGKNELKFTM
                     NNMLNSDSQSRVLRLLVDLSEFEYGLSHWSLFPWDILAQERVYIPEILFEDITITTAQ
                     WNLSAIREEIIHQKIFTGKKDIFNKFRKTYKIPKDIILKYADNELRLDLSIDSDLEIL
                     FKNINKYRFVSLRGIEQGESIFKDNLGNYNTEIVVPILKESNGINNTNDNVQFKNEVK
                     KIYLPFCDWLFFKFYGPKERQEELLNNWKKFWLNLPSELFSQSNMF"
BASE COUNT          716 a          194 c          342 g          587 t
ORIGIN      
        1 tacagtgaat gaatcagagg aagatagaaa cataagtttt tcaaaaccaa ttaagttact
       61 tgtagaaaaa tgtaaaactc gatatataaa atatgaagag ttaatagatg tatttaaaaa
      121 taattatcca gatatagtgt cagacatgct agagtcatat atacacgact tagttacaaa
      181 tgatttttta atttcagatt taagaccacc aatttgtaat atcaattcct tagattatct
      241 actatctaaa ttagaagaag gaacactatg tactgactta actactttaa agaagatgat
      301 tgaagattac aacgatttaa atattgggga aggtagagac ttacttttgc agatttttga
      361 taaaatggaa agtatttata aatatgataa cggtggaaat tacttaaaag ttgattcatt
      421 agcagagtat gaaggcggtt ttaatattct atctgaaacg gctcgggaaa gtatagaacg
      481 actcgcagag atgtttgtaa aattaaatac aaatgtaaag agaagagggg ggaggaaaaa
      541 ttcacttgaa gagtatcagt tgaaatttat tggcaaatat ggagaaaact gctcaattcc
      601 ttttgttaaa gtaataaata aagatgcagg cataggtttt cctgaatatt acaaaggagg
      661 agaaggtgaa gcaatagagt taagcgatcc aataatgcaa atgtttgaaa agaaatatga
      721 ggaagctcta ttaaatggag ggcatatcga attttattca aaagatttag atgattttca
      781 aacagatcaa agcaatagtt tagattcgtt tgaattaaat tttaatataa aaataataaa
      841 taatgatgtg aagttatatt taggagctaa tatcggttct ggacaagcag gtagaagctt
      901 tggaagattc tatggattaa gcgaaacagt aagagaaaca attaagaatt taaaccatca
      961 aaataatacg aatgtagagt tgtcatttgt tcctaaacag ataagacttg cgaatgtaat
     1021 tcaaaattat agtgatgagt cttataatac ttcatttttt accacatcat gggattcaga
     1081 gaacgaactg cgattggagg atatttatat aagatatagt gatggtaagt ttcattttac
     1141 aactatcgat gggaaaaatg agttgaaatt taccatgaat aatatgctaa attccgatag
     1201 ccaaagcagg gttttgagat tgttggttga tttatctgag tttgagtacg gtttatcgca
     1261 ttggtcatta tttccttggg atattttggc tcaggaaaga gtatatattc ctgaaatttt
     1321 atttgaagac attacgatta ccactgctca gtggaattta tcagcaatta gagaagagat
     1381 tatacatcaa aaaatattta ctggtaaaaa agatatattc aataagttta gaaagactta
     1441 taagatacct aaagatatta ttttgaaata tgcagataat gaattgaggt tagatttatc
     1501 tatagatagt gatttagaga ttttatttaa aaacattaat aaatataggt ttgtttcttt
     1561 gagaggaatt gagcagggag agtctatttt taaggataat ttagggaact acaatacaga
     1621 gatagtagtt ccaattctta aggaatctaa tggaataaac aatacaaacg ataatgttca
     1681 atttaaaaat gaagttaaga aaatatattt accattttgt gactggctat ttttcaaatt
     1741 ttatggtcca aaagagcgac aagaagaatt actaaataac tggaaaaaat tttggctaaa
     1801 cctgccatca gaactgttta gccaaagtaa catgtttta
//
LOCUS       DAUDYX010000099         1809 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.100, whole genome shotgun sequence.
ACCESSION   DAUDYX010000099 DAUDYX010000000
VERSION     DAUDYX010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.100"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..1093
                     /locus_tag="VHD02_001862"
     CDS             <1..1093
                     /locus_tag="VHD02_001862"
                     /inference="COORDINATES: protein motif:HMM:NF019348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ5498438.1"
                     /translation="KFLNWRNKPHQRIFFGAPGTGKSYELNKEAKEYFGNNYERVTFH
                     PNYMYGNFVGAFKPFPKVLKNSDGTIKKDEEGNIQESITYEYIPGVLMRQLVRALKCP
                     TQDYLLLIEEINRANVAAVFGDVFQLLDRDGSGESEYFIATSKELQEFLKKEFDDCEL
                     GKNIKEKLGEDFGRLYLPNNLYIWATMNSADQGVMPMDTAFRRRWEFRYLGIDDAAES
                     NKEDFERYKFKINSSEVVNWDLFRRELNKKLSSLNIPEDKLIGPYFVSKAILEDSDLD
                     RLTETIKNKVLMYLYEDAAKAHRPSLFAEGKYATYSSVCKHFDDNALNLFKGNIEIET
                     QSIETNQVHDYSKTDEVSIATEDEVEYES"
     gene            1110..>1809
                     /locus_tag="VHD02_001863"
     CDS             1110..>1809
                     /locus_tag="VHD02_001863"
                     /inference="COORDINATES: protein motif:HMM:NF021104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LlaJI family restriction endonuclease"
                     /protein_id="HEQ5498439.1"
                     /translation="MYIATMQELKPYSFFDLQNEFHMSEEELKNILKSLSLMNIVKKL
                     SKDVSKVELEELLETDSLEKLNTELEGNMYVFKYVGMLVIGNVCLLIYPKYAVSYLND
                     SINNFQKLKQLISVIRKYQSKEQKIGFSSQVDVDNFNLLSIALELIYSYYDHGLYSND
                     KQIIEQNGCGEILWEKTINESTAYFSNGLPIYLDTFTVNQENNEQDYFRRLHAFILSE
                     VCKKLKDILRILDID"
BASE COUNT          689 a          205 c          349 g          566 t
ORIGIN      
        1 gaaatttcta aattggagaa ataaaccaca tcaaaggata ttctttggtg ctcctggtac
       61 tggaaagagt tatgaattaa acaaagaggc aaaagaatac tttggaaata attatgaacg
      121 tgtaactttc cacccaaatt atatgtatgg aaattttgtc ggtgccttta aaccatttcc
      181 taaggtttta aaaaatagtg atggaactat caagaaagat gaagagggaa atattcaaga
      241 atcaataacg tatgagtata ttcctggtgt acttatgaga cagctagtta gagctttaaa
      301 atgtccaaca caagattatt tgcttttaat tgaagaaata aatagagcga atgttgcagc
      361 tgtatttggt gatgtttttc aattattaga tagagatggc tctggggaaa gtgagtattt
      421 tattgcaaca tcaaaggaac tacaagaatt tctaaaaaaa gaatttgatg attgtgaatt
      481 aggaaaaaac atcaaagaaa aattaggcga agattttgga agattgtatt taccgaataa
      541 cctatacatt tgggcaacaa tgaatagtgc tgatcaaggg gttatgccta tggatacagc
      601 atttagaaga agatgggaat ttagatattt aggaattgat gatgcagctg agtcaaataa
      661 agaagatttt gaaagataca aattcaaaat aaattcttct gaagtggtta attgggattt
      721 atttagaaga gagttaaata agaagttatc ttcgcttaac attcctgagg ataaattgat
      781 tggtccgtat tttgtgtcca aagcgatttt agaagattct gatctagata ggcttacaga
      841 aactattaag aataaagttt tgatgtatct atatgaagat gcagctaaag cgcatagacc
      901 atcgttgttt gcagaaggta aatacgctac ttactcaagt gtttgcaaac attttgatga
      961 taatgccttg aacttattta agggaaatat tgaaatagaa acccaatcga ttgagacgaa
     1021 ccaagtacac gattacagta aaacagatga agtatctatt gctacagaag atgaggtaga
     1081 gtacgaatcc tagtgtaggg ggggaattta tgtatatagc aacaatgcaa gaattaaagc
     1141 catattcatt ttttgaccta caaaatgaat ttcacatgag tgaagaagaa ctaaagaata
     1201 tattaaagtc cctttctttg atgaatattg taaaaaagtt atcaaaagat gtatcgaaag
     1261 tagaattaga ggaattactg gaaacagata gtttagaaaa acttaatact gaactagaag
     1321 gaaatatgta tgtgtttaag tatgttggaa tgcttgttat aggaaatgtg tgtttactta
     1381 tttaccctaa atatgcagtt tcttatttga atgattcaat aaataatttt caaaaactta
     1441 agcaattaat atcagtaatt agaaagtatc aatcaaaaga gcaaaaaata ggttttagta
     1501 gtcaagtaga tgtcgataat tttaatttgt tatcaattgc attagaactg atttacagct
     1561 attacgatca tggactttat agtaatgata aacaaattat tgaacaaaat ggatgcgggg
     1621 agattttgtg ggagaaaact attaatgaaa gtacagctta tttttcgaat ggattaccga
     1681 tatatctaga tacatttaca gttaatcaag aaaataatga gcaggattat ttccgaagat
     1741 tacatgcatt tattttgtct gaagtatgca aaaagctcaa agacattttg cgcatactcg
     1801 atatagata
//
LOCUS       DAUDYX010000100         1722 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.101, whole genome shotgun sequence.
ACCESSION   DAUDYX010000100 DAUDYX010000000
VERSION     DAUDYX010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.101"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            144..1064
                     /locus_tag="VHD02_001864"
     CDS             144..1064
                     /locus_tag="VHD02_001864"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEQ5498440.1"
                     /translation="MSNEFINFEKISRESWKTLHQKAKALLTQEELKSITSLNDNISI
                     NDVIDIYLPLINLIQVYKIAQENLSFSKSLFLKKDIQLRPFIIGISGSVAVGKSTTSR
                     LLQLLLSRTHPNSQVELVTTDGFLYPNQFLIEQGLLNRKGFPESYNMELLLDFLDSIK
                     NGQTAFAPVYSHDIYDIIPNQKQSFNNPDFLIVEGINVFQNQQNNRLYMSDYFDFSIY
                     IDADSSHIETWYIERFLSILKLAKCDPHNYYAQYAQLPRSEAIAFARNVWKTVNLENL
                     EKFIEPTRNRAELILHKSADHKIDEIYLKK"
     gene            1118..1366
                     /locus_tag="VHD02_001865"
     CDS             1118..1366
                     /locus_tag="VHD02_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEQ5498441.1"
                     /translation="MEVKTLANIKSAIKRAELNVKANEKNSAQKSAMRTAIKAFEANP
                     SEELFRAASSSIDKAESKGLIHKNKASRDKARLAAKLG"
     gene            complement(1491..>1722)
                     /locus_tag="VHD02_001866"
     CDS             complement(1491..>1722)
                     /locus_tag="VHD02_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ5498442.1"
                     /translation="TDKNLLISVIDNGPGITDEEKKKIFDRFYRVDKARTRQTGGFGL
                     GLALAQQIVMSLKGNITVKDNDPKGSIFEVKL"
BASE COUNT          604 a          309 c          249 g          560 t
ORIGIN      
        1 gaatatagca ggataattgt actaggaatg tgtggctttt ttgagttaat tgtattaatt
       61 ttcgtgtaaa tactaacgaa aaattattta ttttttaact aaaatggtat aatgtaagca
      121 ctgtcaaatg attaggagct accatgtcaa atgaatttat caactttgaa aaaatttcta
      181 gagagtcttg gaaaacatta catcaaaaag caaaagctct tctcacacaa gaggaactta
      241 aaagtattac tagcttaaat gataacatca gtattaacga tgtcattgat atctaccttc
      301 cattaattaa tctgatccag gtttacaaaa ttgcgcaaga aaatctttct ttttctaaaa
      361 gcctttttct aaaaaaagac attcaattaa ggccttttat tattggaatt tctggttcag
      421 tagctgttgg taaatcaaca actagtcgtc ttttacaact tttattatca cgaacgcacc
      481 ccaatagtca agtcgaactg gtaacgactg atggtttttt atatcctaat cagtttctta
      541 ttgaacaagg actgttaaat cgtaaaggat ttccagaatc ttataacatg gaacttctct
      601 tagattttct agattccatc aaaaatggtc aaactgcttt tgcacccgtc tattctcatg
      661 atatttacga tattatccca aatcaaaaac aatcatttaa taaccctgac ttccttatcg
      721 tcgaaggtat taatgttttt caaaatcaac aaaacaatcg tttatatatg agtgattact
      781 ttgatttttc tatttatatt gatgcagaca gcagtcacat cgaaacctgg tacattgaac
      841 ggtttctaag cattttaaaa ttagcaaaat gtgaccctca taattattat gcccaatatg
      901 cacaattacc tcgatctgag gctatagcat ttgcgcgcaa cgtttggaaa accgttaatt
      961 tagaaaatct tgaaaaattc attgaaccta cacgtaaccg tgctgaatta attttacata
     1021 aaagtgctga tcataaaatt gatgaaattt acctcaaaaa gtgataaagg gttgccaaac
     1081 gcatatattt tctatataat aatgtagtta gattaatttg gaggtgaaaa cattggcaaa
     1141 tattaaatca gctattaaac gcgctgaact taacgttaaa gcaaacgaaa aaaactcagc
     1201 acaaaaatca gctatgcgta ctgctatcaa agcatttgaa gctaacccat ctgaagagct
     1261 tttccgcgct gcttcttcaa gcatcgataa agctgaatca aaaggtttga ttcacaaaaa
     1321 caaagcaagc cgcgataaag cacgccttgc tgcaaaactt ggctaataaa tatccaatga
     1381 aaactccagc tggagttttt ttatacggta aaaactcaaa aaatacaata aagcgttgac
     1441 agttaaaaat aactttaaaa gggctgacct tattggtcgc cctttttagc ttataatttg
     1501 acttcaaaaa tactaccttt aggatcatta tcctttactg taatatttcc ttttaaagac
     1561 atcacgattt gctgagctaa agccaacccc aagccaaatc cacctgtttg ccgtgttcta
     1621 gctttgtcaa ctcgataaaa acgatcaaaa atctttttct tttcttcatc tgttatccct
     1681 ggaccattat caataacaga aattaataaa tttttgtccg tt
//
LOCUS       DAUDYX010000101         1717 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.102, whole genome shotgun sequence.
ACCESSION   DAUDYX010000101 DAUDYX010000000
VERSION     DAUDYX010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.102"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..120
                     /locus_tag="VHD02_001867"
     CDS             <1..120
                     /locus_tag="VHD02_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEQ5498443.1"
                     /translation="YDSISKSEVVWTDDNIIVEHTTRKLYSPNPRIEVKINEL"
     gene            117..473
                     /locus_tag="VHD02_001868"
     CDS             117..473
                     /locus_tag="VHD02_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498444.1"
                     /translation="MTLVDDFYKQMEPSIKAFLDDNITIADKEEADRVYRSVKCYKKL
                     NRLPPPDVLEWFQRIYTTEEMIMLIKQSYRLKQKKTDEDDKIYEKWMFKNYGDVKLVK
                     KIKRINALAKAREMGL"
     gene            715..999
                     /locus_tag="VHD02_001869"
     CDS             715..999
                     /locus_tag="VHD02_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEQ5498445.1"
                     /translation="MLTEDTFKKIEELEAACQDTTDNIKKPSHYQGRHGMEAIDVVKN
                     FSACPEHEEGFYWGNAVKYLLRYHAKNGVEDLKKARQNLDWLIEKLEEVE"
     gene            996..1265
                     /locus_tag="VHD02_001870"
     CDS             996..1265
                     /locus_tag="VHD02_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498446.1"
                     /translation="MKKPNRYPYSKSKFNGCIYQLHSASFKDEQYVEDLKSCGIHYQI
                     TKIGYFPDIFIKIDNLEQLQILIDKTGHDLILSKDQIWIYDDYME"
     gene            1275..1679
                     /locus_tag="VHD02_001871"
     CDS             1275..1679
                     /locus_tag="VHD02_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498447.1"
                     /translation="MIPKFRAFNKKTKKMYSIDGFKSSERKIYRCSLADDEFRSGRLE
                     TFHFVEDNLDDYVLMQSTGLFDKNGVEIFEGDVVEYDDGEYLFAGKVVKTVFGTYVKS
                     YSFFSFEDFSDENTMTADVEIIGNIYEESVEE"
BASE COUNT          634 a          257 c          365 g          461 t
ORIGIN      
        1 tatgacagca tatcaaaatc agaggttgtg tggacagatg acaacatcat tgtcgagcac
       61 actacacgta agctgtatag cccaaatccc agaatcgagg taaaaataaa cgaattatga
      121 cactagtaga tgatttttac aaacaaatgg agccgtcaat taaagcgttt ttagacgata
      181 acattaccat cgcagataaa gaagaagctg acagagtcta tagatctgtc aaatgctata
      241 aaaaactaaa cagattgccg ccgcctgatg tattggagtg gttccaacga atctacacga
      301 cagaggaaat gataatgtta atcaagcagt cttaccgcct taaacaaaaa aagacagatg
      361 aggatgacaa gatttacgaa aagtggatgt ttaaaaacta cggtgacgtt aagctcgtta
      421 aaaaaatcaa acgcataaac gcattagcta aggctcggga gatgggtcta tgaaaagaca
      481 cagacagtgg cataacgata ttaaatatac acctagatct tacgataatc tgttgcctta
      541 cgatatatca gagctgttaa tagctcacgg atgcaaaata aagatgtctg atgaggtttt
      601 agcagacaag ataggtattt atacttggca attaaaagcg ctcttagaac gcagaatatt
      661 gccaaatgag agcgtgtgta aattgattat aaattttttg agagaggtgg agagatgctg
      721 acggaagata cgtttaaaaa aattgaggag cttgaagctg cttgtcagga tacgacagat
      781 aacattaaaa aaccatcaca ctatcaaggc aggcatggca tggaagcaat cgatgtggtt
      841 aaaaactttt cagcttgtcc agagcacgag gaaggttttt actggggcaa tgctgttaag
      901 taccttttgc ggtatcacgc taaaaatggt gttgaagacc ttaaaaaagc acggcagaac
      961 cttgattggt taatcgaaaa gttggaggaa gtggaatgaa gaaaccaaat cgctatccgt
     1021 acagtaaatc aaaatttaat ggttgtattt accagttgca ttcagccagc tttaaagatg
     1081 aacaatatgt tgaagattta aaatcatgcg gcatacatta tcaaattaca aaaattggtt
     1141 attttcctga tatttttata aaaattgata atctcgaaca attacaaata ttaatagata
     1201 aaacaggaca cgatttaata cttagtaaag accaaatttg gatttatgat gactatatgg
     1261 aatgaggagt aataatgata ccaaaattta gggcatttaa taaaaagacc aaaaaaatgt
     1321 atagcattga tggctttaaa tcaagtgaac gcaaaatata cagatgcagc ttagctgatg
     1381 acgagtttcg ctctggtcgc ttagagacgt ttcattttgt cgaggataac cttgacgatt
     1441 atgtcctcat gcagtccaca gggctatttg ataaaaacgg tgttgagatt tttgagggag
     1501 atgtggtcga atatgacgac ggagaatatt tgttcgctgg aaaagtagtt aaaacagtat
     1561 ttggaacata tgtaaaatct tacagttttt tctcgtttga agatttttcg gacgaaaata
     1621 caatgaccgc agacgttgaa atcatcggca acatttacga agaaagcgtg gaagaatgag
     1681 aaaatatatt gaatttaagg acgaatggaa aagtgca
//
LOCUS       DAUDYX010000102         1667 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.103, whole genome shotgun sequence.
ACCESSION   DAUDYX010000102 DAUDYX010000000
VERSION     DAUDYX010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.103"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            102..581
                     /gene="rlmH"
                     /locus_tag="VHD02_001872"
     CDS             102..581
                     /gene="rlmH"
                     /locus_tag="VHD02_001872"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEQ5498448.1"
                     /translation="MKVKLICVGKLKERYLKDGISEYQKRLSRFCQFEMIELTDERTP
                     DKASFADNQLIMSKEAQRIHKKIEERDFVIALAIEGKQFPSETFSELISGVTVKGYST
                     ITFIIGGSLGLDSIIKKRANMLMSFGLLTLPHQLMRLVLTEQIYRAFMITQGSPYHK"
     gene            complement(739..812)
                     /locus_tag="VHD02_001873"
     tRNA            complement(739..812)
                     /locus_tag="VHD02_001873"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(776..778),aa:Arg,seq:tct)
     gene            977..1667
                     /locus_tag="VHD02_001874"
                     /pseudo
     CDS             977..1667
                     /locus_tag="VHD02_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871253.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          585 a          279 c          312 g          491 t
ORIGIN      
        1 ctaataaaga cctttgtaca aaggttttta ttagtagttg aagaaaaata acttgtggat
       61 aaattgtgta aaaagtaatg ttcttgtgat acaattaagc tatgaaagtg aaactaattt
      121 gtgttggaaa actaaaagaa agatacctca aagatggtat ttcagaatat caaaaaagac
      181 tttcccgttt ttgtcaattc gaaatgattg agttaactga tgaaagaaca ccagataaag
      241 caagttttgc tgataatcaa ttgattatga gtaaagaagc tcagcgaatc cataaaaaaa
      301 ttgaggaacg tgattttgta attgcattag ctattgaagg aaaacagttt ccttcagaga
      361 catttagtga attaatatca ggggttactg ttaagggata ttcaactatc acgtttatta
      421 ttggtgggag tcttggatta gattcaatta ttaaaaaaag agctaatatg ctaatgagtt
      481 ttggattgtt aaccttacct catcagttaa tgcgtctagt ccttactgag caaatttatc
      541 gtgcttttat gattacacaa ggcagtcctt atcataagta agagagtgtg aactggaata
      601 actaactctt accactttat tagataatag ttcggtataa ttaaaagcaa atattaaaaa
      661 aagacgtact aaatcctatc ttttcaatac actaaggtaa atacaaaaaa aagcactaaa
      721 acagtgcttt tttatctttg atcccagcag gattcgaacc tgcgaccgtt cgcttagaag
      781 gcgaatgctc tatccagctg agctatgaga ccttaaccac atcattttaa caaaaattat
      841 ctaaaactgt caatatttat tttgaaaaaa ctagaaaata acattatatc ccgcgttgta
      901 aaatgaagtg caacaaaaaa gacatgttca tctgatatac tagaattccc caattcagta
      961 tggaaagcag atgaacatgt ctaatattaa ttctaccaga aagtcatcct attctcatct
     1021 ttcagccaca gaacggggtg aaattgctgc ttatcttaaa atgggaaaga aacccgttga
     1081 gattgctcga cttctggggc gtcactgttc tacaatctgc cgagaaataa aacgtggttc
     1141 agtagatcag gtaaaggata agaatggaaa acaaaccttc ttcaacgcct atttcgctga
     1201 tagtgggcaa cgtgtctatt aaaccaatcg atttgcctaa ggtggttagt atcagaaaac
     1261 gttctaagaa agtcatgaag acgaataaga agactttagg taaatctatc gaagaacgtc
     1321 cagaatatat taatgatcgt tctgaatttg ggcattggga gattgatttg gttctcggca
     1381 agaaaaccaa aagtgaagct gttatgttga ctttggtaga gcgccagaca cgctatgcac
     1441 taggagttaa gctagaagac aagcagtccc aaaccattaa ccgtgctgtc acgcatctca
     1501 tcagtcagta tcctattgca tccatcacag cggataatgg ttctgagttt agtttactct
     1561 cagacttaaa agctgttgac gtttactttg cacatcccta ttcttcacat gagagaggaa
     1621 caagcgagaa cttcaatggc ctcctcagag aatatgtccc taaagga
//
LOCUS       DAUDYX010000103         1556 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.104, whole genome shotgun sequence.
ACCESSION   DAUDYX010000103 DAUDYX010000000
VERSION     DAUDYX010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.104"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..600
                     /locus_tag="VHD02_001875"
     CDS             <1..600
                     /locus_tag="VHD02_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEQ5498449.1"
                     /translation="TGKYPQFDSTGAVASTHIIITAEDGSVISQLVKQDLISTNDTEI
                     IKATLEEFKKSEYVEIAMGEAVQKVDDLEKISQETAKTAKTAQTAAGLAKVSAERTQR
                     MINLQTIHMLTSGGKIDSDIYKGMLELIEPAKKGEYQAYDVFTVVDSTKEEDGEAGEG
                     NLVFVHVNEPFTYEAQTLEELESEAKVTVIKYADLVKQD"
     gene            610..882
                     /locus_tag="VHD02_001876"
     CDS             610..882
                     /locus_tag="VHD02_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498450.1"
                     /translation="MATELIFGVGGFILAIVTTYNIFNAKSIKHATDITLLQSEVEHL
                     KIVTRQNARRLEEHDEQNKTLITMTEQIKNLNREVRELKDIMKGEA"
     gene            879..1106
                     /locus_tag="VHD02_001877"
     CDS             879..1106
                     /locus_tag="VHD02_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEQ5498451.1"
                     /translation="MINLKLRLQNKVTLMAILGAIFLLAQQLGIKLPSNIADIANTAV
                     TLLVLLGVVTDPTTKGLSDSEQALTYHEPKK"
     gene            1222..>1556
                     /locus_tag="VHD02_001878"
     CDS             1222..>1556
                     /locus_tag="VHD02_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEQ5498452.1"
                     /translation="MTFLDKIKQGCLDGWTKYKILPSLTAAQAILESGWGKHAPHNAL
                     FGIKADASWTGKSFDTKTQEEYQPGIVTDIVDRFRAYDSWTDSIIDHGKFLNDNPRYK
                     AVIGETDYK"
BASE COUNT          514 a          292 c          344 g          406 t
ORIGIN      
        1 acaggaaaat acccacaatt tgacagcaca ggagcagtcg caagcacaca tattattatc
       61 actgctgagg atggctcagt catctctcaa cttgttaagc aagacttaat ctcgactaat
      121 gacacagaga ttatcaaagc tactttggaa gaatttaaaa aatctgaata cgttgaaatt
      181 gcaatgggcg aagccgtgca aaaggtagac gaccttgaaa aaatctcaca ggaaactgct
      241 aagactgcca agactgctca aacagccgca ggattagcta aggtgtccgc agagcgtaca
      301 cagcgaatga ttaacttgca aaccatccac atgttaacga gcggcggcaa gattgattct
      361 gacatttata aaggcatgct tgagctaatc gagccagcca aaaaaggtga gtatcaagcc
      421 tatgatgtct ttacggtggt cgacagtact aaagaggaag acggtgaagc aggcgaaggc
      481 aatcttgtct ttgtacatgt caacgagccg tttacttatg aggcacagac cttagaggag
      541 ctagagtcag aagccaaagt aacagttatc aagtacgctg atttggttaa acaagattag
      601 aggtgtttta tggcaacaga gttgatattt ggcgtcggtg gctttatttt agctatcgtc
      661 acgacttaca atatttttaa tgcaaaatct atcaagcatg cgacagatat tacattgttg
      721 cagtctgagg ttgagcattt aaaaattgtc actcgtcaaa atgctaggcg tcttgaggag
      781 catgatgagc aaaacaaaac gctcatcaca atgacagagc aaattaaaaa cctcaatcgt
      841 gaggtaagag aacttaaaga tattatgaaa ggcgaagcat gatcaattta aaattacgac
      901 tacaaaacaa agtaaccttg atggctattt taggagctat ctttttactg gcacagcaat
      961 taggtattaa actaccatca aacatcgcgg atattgcaaa cacagctgta acgcttttgg
     1021 tattgctcgg tgttgtcaca gatccaacca cgaaaggcct gtcagatagt gagcaagcat
     1081 tgacttacca tgagcccaaa aaataggagg agccatgcga gcaatcacac gattagcgtt
     1141 aatactagca atcgcaatac tgtatgtgcc attatctgtg gttgctttga ttttttatcc
     1201 atttttagat aaggaggaca gatgaccttt ttagataaaa ttaaacaagg ctgtttagat
     1261 ggctggacta agtataaaat cttgccatcc ttgacagctg ctcaggctat tttagagagc
     1321 ggttggggca aacatgcacc acataacgct ttatttggta ttaaggctga tgcgagctgg
     1381 acaggtaagt cttttgacac taaaactcag gaggagtacc agcctggtat cgtcacggat
     1441 attgtggacc gatttagggc ctatgatagt tggactgaca gtattattga tcatggcaaa
     1501 tttttaaacg ataatccacg ctacaaagca gttatcggtg agactgacta taaaaa
//
LOCUS       DAUDYX010000104         1510 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.105, whole genome shotgun sequence.
ACCESSION   DAUDYX010000104 DAUDYX010000000
VERSION     DAUDYX010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.105"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..120
                     /locus_tag="VHD02_001879"
     CDS             <1..120
                     /locus_tag="VHD02_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEQ5498453.1"
                     /translation="YDSISKSEVVWTDDNIIVEHTTRKLYSPNPRIEVKINEL"
     gene            117..473
                     /locus_tag="VHD02_001880"
     CDS             117..473
                     /locus_tag="VHD02_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498454.1"
                     /translation="MTLVDDFYKQMEPSIKAFLDDNITIADKEEADRVYRSVKYYKKL
                     NRLPPPDVLEWFQRIYTTKEMIMLIKQSYRLKQKKTDEDDKIYEKWMFKNYGDVKLVK
                     KIKRTDALERARRMGL"
     gene            715..951
                     /locus_tag="VHD02_001881"
     CDS             715..951
                     /locus_tag="VHD02_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEQ5498455.1"
                     /translation="MIEELVHKPKHYNHGEIDLIESWYKTYPWDQFVAIMESHIDKYI
                     KRHRYKGNATQDLEKALEYTKRLIEYWLIESVEE"
     gene            948..1118
                     /locus_tag="VHD02_001882"
     CDS             948..1118
                     /locus_tag="VHD02_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498456.1"
                     /translation="MKRYIEVKDEWKSATDHLNDFIDKNKYAKVTVVGYQVVQLSPYG
                     RDLTYILAEVEE"
     gene            1115..1399
                     /locus_tag="VHD02_001883"
     CDS             1115..1399
                     /locus_tag="VHD02_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498457.1"
                     /translation="MTIDEALQNLRDNFNKIMNVLKNDWKALLFLAIAIFGMMVTVSY
                     FSYRDARQYYESQITGLRTQLSRTQKQLKRASEDRARQTKRIAELTHNGG"
BASE COUNT          574 a          250 c          321 g          365 t
ORIGIN      
        1 tatgacagca tatcaaaatc agaggttgtg tggacagatg acaacatcat tgtcgagcac
       61 actacacgta agctgtatag cccaaatccc agaatcgagg taaaaataaa cgaattatga
      121 cactagtaga tgatttttac aaacaaatgg agccgtcaat taaagcgttt ttagacgata
      181 acattaccat cgcagacaaa gaagaagctg acagagtcta tagatctgtc aaatactata
      241 aaaaactaaa cagattaccg ccacctgatg tattggagtg gttccaacga atttacacga
      301 caaaggaaat gataatgtta atcaagcagt cttaccgcct taaacaaaaa aagacagatg
      361 aggatgacaa gatttacgaa aagtggatgt ttaaaaacta cggtgacgtt aagctcgtta
      421 aaaaaatcaa acgcacggac gcactagaaa gagctcggag aatgggtcta tgaaaagaca
      481 cagacagtgg cataacgata ttaaatatac acctagatct tacgataatc tgttgcctta
      541 cgatatatca gagctgttaa tagctcacag atgcaaaata aagatgtctg atgacgtttt
      601 agcagataag ataggcattt atacttggca attaaaagcg ctcttagaac gcagaatatt
      661 gccaaatgag agcgtgtgta aaacgatagt aaattatttg agagaggtgg agtgatgata
      721 gaagaattag tacataaacc aaagcattat aatcacggag aaattgattt gattgagtcg
      781 tggtataaga cgtacccatg ggaccagttt gtcgcaatca tggaaagtca cattgataaa
      841 tacattaaac gacatagata caaaggcaat gcgactcagg atttagaaaa agcactcgaa
      901 tatacaaagc gtctcattga atattggtta atcgaaagcg tggaagaatg aagagatata
      961 ttgaagttaa ggacgaatgg aaaagtgcaa cagaccacct gaacgatttt atcgacaaaa
     1021 acaagtacgc aaaagtgaca gttgttggtt atcaagtcgt acaactttct ccttacggaa
     1081 gagatttgac ttatattttg gcagaggtgg aagaatgacg atagatgaag cgttgcaaaa
     1141 tttacgtgat aactttaata aaataatgaa tgtcctaaaa aacgattgga aagcactatt
     1201 gtttcttgca atcgcaatat ttgggatgat ggtaaccgtg tcgtatttta gctatcgcga
     1261 cgcacgacaa tattacgagt cgcaaatcac aggactacgt acacagctaa gcaggacaca
     1321 aaagcagctt aaacgtgcta gcgaagatag agctagacag acaaagcgaa ttgcggaact
     1381 tacgcacaac ggagggtaac atgattaaga tcgatgagat acatcgcata ctaggcatcg
     1441 acgaagttta taaagcaccc aaacgactta cggacatact ctttgataaa gatagtcgtg
     1501 aggatatatt
//
LOCUS       DAUDYX010000105         1488 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.106, whole genome shotgun sequence.
ACCESSION   DAUDYX010000105 DAUDYX010000000
VERSION     DAUDYX010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.106"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            1..456
                     /locus_tag="VHD02_001884"
     CDS             1..456
                     /locus_tag="VHD02_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEQ5498458.1"
                     /translation="MEEAKELVDNSKFYGKTSSVIKAEVRDIIDQLNQPKPEVPQCVA
                     DWIEECKEEDLTLKGLFSNSDMPAKIFDWIFGSDENCRLMAEAWINGYTVEKEKLYTV
                     DLPNGQPLVRGINTLYFSQNLATENVKLTESEIRKDFEWAWQFAEEVTE"
     gene            906..1346
                     /locus_tag="VHD02_001885"
     CDS             906..1346
                     /locus_tag="VHD02_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEQ5498459.1"
                     /translation="MTFFPEIDIQKTKSNAKRKLREYPRWRRIANDVDTQKVTATYSF
                     EPRQPHGTPSKPVERLALNRVSAEQELDTIERAVNGIFDPEYRLILIDKYLLTYPKTD
                     CDIYTKLGYEKSQYYNMLDNALLSFSELYKEGMLLVEKLEKSWN"
BASE COUNT          526 a          261 c          308 g          393 t
ORIGIN      
        1 atagaagaag cgaaagaatt agtagataat tcaaaatttt atggaaagac tagcagtgtt
       61 ataaaagccg aggttcgcga cattatagac cagttaaacc aaccaaaacc agaagtaccg
      121 cagtgtgtgg cggattggat agaagagtgc aaagaagaag atttaacttt aaaaggcttg
      181 ttttctaaca gcgatatgcc tgcaaaaatt tttgattgga tttttggtag tgacgaaaat
      241 tgtaggttaa tggctgaagc atggattaac ggctacacag tagaaaaaga aaagctctac
      301 actgtcgact taccaaacgg tcaaccttta gtgcgcggca taaacacttt gtattttagt
      361 caaaatctag caaccgaaaa tgtaaaactc accgaatccg aaatccgcaa agattttgaa
      421 tgggcgtggc agtttgctga agaggtgact gaatgactga agagttagga gtgttatata
      481 gcgaaaaatg gcataagtat tatttatata aaacttgcag gtatatatct tatgttgata
      541 atccacatca ggctactaaa tgcacccgca aacaagtaga acagtttcca cagtttaaat
      601 gggtatcgct gacaaaatta taaccccacg caagcgctca agagcctgcg atggctctgt
      661 gggggtggac cgaaattaaa aaatagaaac gagaacctcc ttacaccaaa acaaatctaa
      721 agcgggttat cggtcatccg tgattatcca aggcgtcgct aatgctttaa cacgacatcg
      781 tgcgcctgtg tcaaaaaaca aaggaaagag aggacttttc tccacaaaac aaaaagacgt
      841 ccatacggaa cgcccccttg gttaaattta agcttaaata aattatacca cattgggggg
      901 ctttcatgac gttttttcct gagattgata tccaaaaaac aaaatccaac gccaagcgta
      961 aattgagaga gtatccacgc tggcggagga tagctaatga tgtagatact caaaaagtta
     1021 cagctactta ctcctttgag ccaagacaac cgcatggaac gccaagcaag cctgttgaga
     1081 gactagcgct caaccgtgtg tcggcagaac aggagctgga tacgattgag agagcagtca
     1141 acgggatatt tgatccagag tatagattga tactgattga caagtatttg ctcacatatc
     1201 caaagactga ttgtgacatt tatacaaaac ttggttatga aaaaagccag tattacaaca
     1261 tgctagataa tgctttgttg tcgttctcag agctatataa agagggaatg ttgcttgtcg
     1321 aaaaattgga aaaaagctgg aattaatatg gaataattat gtactttata tataattatt
     1381 catgttatta tagtactatc aaaataacaa gaagagataa ccttttaacc aattggctat
     1441 ttatttagtc gtcaacttta actacaatca aacttgttat tttgtagc
//
LOCUS       DAUDYX010000106         1426 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.108, whole genome shotgun sequence.
ACCESSION   DAUDYX010000106 DAUDYX010000000
VERSION     DAUDYX010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.108"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            157..930
                     /locus_tag="VHD02_001886"
     CDS             157..930
                     /locus_tag="VHD02_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004270277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ5498460.1"
                     /translation="MKKKERHEKILDILKVDGFIKVKDIIDEMNISDMTARRDLDTLA
                     DKGLLIRTHGGAQYLDYSSAKDEGHEKTHTEKKVLQTTEKKQIAQRAKNIIKDGETVF
                     IGPGTTLEHLAVELKNRNIRVITNSLPVFLILHPSTTIDLILIGGECREITGAFVGAI
                     AINNLETLTFSKAFVSANAVYNNSIATYSDLEGEVQRVALNNAVEKILLVDSTKFESY
                     DFYNFYQLDQVDLIVTDKNVTTDIIEKYKKFSKIIIADK"
BASE COUNT          477 a          214 c          247 g          488 t
ORIGIN      
        1 atctctattt attgtaaacg ttttatttaa aacgttttct attttgttaa tttttgtttt
       61 ttccaatatt tatgtttgtt ttgtttgatt tttttattga tattatgtta tgataccttt
      121 aatagttata ccctaagaca ctgataggag ataagaatga aaaagaaaga gcgtcatgaa
      181 aaaattctcg atatcttaaa agttgacggt tttataaaag taaaagatat tatcgatgaa
      241 atgaacatct cagatatgac cgctagacga gatctcgata cattggcaga taaaggatta
      301 cttatcagga cccatggtgg tgctcaatat ttggattatt cttcagcaaa ggacgaaggt
      361 cacgagaaaa cacacactga aaagaaggtc ttacagacta ccgaaaaaaa acaaattgca
      421 caaagagcta aaaacattat taaagatggc gaaactgtct ttataggtcc agggacaaca
      481 ttagagcatc tagctgtcga attaaaaaat agaaatatca gggtcattac aaatagcctt
      541 cctgtttttt taattttgca cccgagcact actattgatt tgattttaat tggtggtgaa
      601 tgtcgtgaaa ttactggagc ttttgttggt gctattgcca ttaataacct agaaacattg
      661 acattttcaa aagcttttgt cagcgcaaat gcagtttaca ataattctat tgcgacgtac
      721 agtgatctcg aaggagaagt tcaaagggtt gcattgaata atgctgttga gaaaatcctt
      781 ctagttgata gtaccaagtt tgaaagttat gacttctata atttctacca actagatcaa
      841 gtggacttaa ttgtgactga taaaaatgtg acaactgata tcatagaaaa gtacaagaaa
      901 ttctcaaaaa tcataatcgc tgacaagtaa aagacctact catattaaaa agcacttcag
      961 ggtttagata tatcccctgt caagtaaaca gggagcatat ctgaaagtag tgtttgtgtt
     1021 ataaagaagt ttcttgttaa cttattttct ggtttttttc agacttatca gtcttgtact
     1081 gttccttttt ctgatgaata acgtggaaat aggcagacag ctatatattt cacctctaga
     1141 atggttgcca ttactgtcat caagacatgc ttgttcttta tcgtatggag tgaagctttc
     1201 ttttagacat caaaatacct cttaagtaga ttttggttcg ttaccatgtc tacttaagag
     1261 gtgtcttcaa agcgctttaa aacgttgata taacagtata ttaacgtttt gagaattgtg
     1321 aagctttacg agctttttta agacctggtt tttggttcac tccattattt aaagtcaaat
     1381 ccctttaaaa tcaatgtttc taaaatgaaa aatcactaaa aatctt
//
LOCUS       DAUDYX010000107         1399 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.109, whole genome shotgun sequence.
ACCESSION   DAUDYX010000107 DAUDYX010000000
VERSION     DAUDYX010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.109"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..313
                     /locus_tag="VHD02_001887"
     CDS             <1..313
                     /locus_tag="VHD02_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEQ5498461.1"
                     /translation="SKIYYIKTDKKEKQIYHSIWNDSLASLYLNELLGRHISGQVACF
                     QSDNGKYFSTMPLINYEKNNRVREQIVKGIFAKQKLFKITAATPAKIAEAIIENVKKN
                     S"
     gene            368..1357
                     /locus_tag="VHD02_001888"
     CDS             368..1357
                     /locus_tag="VHD02_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEQ5498462.1"
                     /translation="MFVIGIPTLNEADNISRLVKQIDEYAVNLGKEIIIINSDSKSTD
                     GTPQIFLETKTYNTKVSIVSEAKGKGYNVRNIFEYAINHVPNFSGLILIDGDVVSMKK
                     MWLEKMFIAIESGNDLIIPNYARKSFEGNATNHFIYPMLVKFFKRDMPYQCISGDFGF
                     SRGLIKDLTLKCNWHKYTLGYGIDIFLTLTAILKSYKIKEIDLQSKIHKKSFEKIEKI
                     FLEVSQSFFETINDNSLNQDKLRLNINFESHSRQFIKSSDILSSNDIENLKLRALFLL
                     QEEKQYPHGLSEVEWDGILSNTINNIYRYSSEEHSLYLLPLYLLRVYNYLKYN"
BASE COUNT          536 a          153 c          248 g          462 t
ORIGIN      
        1 ctcaaaaatt tattatataa agacagacaa gaaagaaaag caaatttatc attcaatttg
       61 gaatgatagt ttagcaagtt tatatctaaa tgagctattg ggaaggcata tttctggaca
      121 ggttgcatgt tttcaatcag ataatggaaa atatttttct acaatgcctt tgataaatta
      181 tgaaaaaaat aatcgggtac gagaacagat agtgaagggt attttcgcta aacaaaaatt
      241 atttaaaatt acagcagcta cgccagctaa gattgcagaa gcgattatag aaaatgttaa
      301 gaaaaactca tagtgtgtgt gaatttgggg attaatatga agtattgaaa aaggagtaag
      361 agtaattatg tttgtgatag gcattccaac tttaaatgag gcagataata tcagtagatt
      421 agttaaacaa attgatgagt atgctgtaaa tttagggaaa gaaattatta ttattaattc
      481 ggatagtaaa agtacagatg gaactccgca gattttttta gaaaccaaaa cttataacac
      541 caaagtttcg atagtatcag aagctaaagg aaaaggttat aatgtgagaa atattttcga
      601 atatgcaata aatcatgtgc cgaatttctc cggtttaatt ttgattgatg gagacgttgt
      661 ctcaatgaaa aaaatgtggt tagaaaagat gtttatagca attgaatctg gaaatgatct
      721 tataatccca aattatgcta gaaaatcttt tgaaggaaat gcgacaaacc attttattta
      781 tcccatgtta gtgaaatttt tcaaacgtga tatgccttat caatgcatct ctggtgattt
      841 tggattttca agaggattga taaaagattt aactttgaag tgtaattggc acaaatatac
      901 attaggttac gggatagata tttttttaac cttaacagct atattaaagt catacaaaat
      961 taaggaaatc gatttacaat caaagattca taaaaaaagc tttgagaaaa ttgaaaaaat
     1021 ttttttagaa gtttcacaaa gtttcttcga aactattaat gataattctt tgaatcaaga
     1081 taagttacgt ttaaatataa attttgaaag tcattcaaga cagtttatta agtccagtga
     1141 tatcctatcc agcaatgata tcgagaattt aaagttaaga gcgttgtttt tattacagga
     1201 agaaaaacag tatccacatg ggctgtcaga ggtagaatgg gacggaattt tgtctaatac
     1261 aattaacaat atttatagat atagtagtga ggaacattcg ttatatttat taccacttta
     1321 tttattgaga gtatacaatt atctaaaata caactaagtt ttcaattttt attaatcttg
     1381 agaagaagtg tgattggtg
//
LOCUS       DAUDYX010000108         1391 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.110, whole genome shotgun sequence.
ACCESSION   DAUDYX010000108 DAUDYX010000000
VERSION     DAUDYX010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.110"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            232..465
                     /locus_tag="VHD02_001889"
                     /pseudo
     CDS             232..465
                     /locus_tag="VHD02_001889"
                     /inference="COORDINATES: protein motif:HMM:NF012711.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            586..>1391
                     /locus_tag="VHD02_001890"
     CDS             586..>1391
                     /locus_tag="VHD02_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEQ5498463.1"
                     /translation="MKITVVGIGYVGLSIGLLLAKEHDVTFFDIDNKKIDLINKRQSS
                     LKEAAINKLLCKAKNINATSSEELAYKDATFIILSLPTNLKVNKLDTSIIEISVSNIL
                     KINKKATIVIKSTVPIGFTEYLRNRFHYNDIIFSPEFLREGSTIHDQLYPSRTIVGNE
                     SRNSQLFLDILTDISVEKDSPSLLVGSSEAEAIKLFSNAYLAQKIAFFNELDTFAEMQ
                     NLDSKKIIEAMGYDQRIGNSHNNPSFGFGGYCLPKDIKQLEYHFKEIPAP"
BASE COUNT          503 a          186 c          235 g          467 t
ORIGIN      
        1 agtatgtaag taaatttatt tataatattt ttgatacttt gagtagtaac gataatattt
       61 aattttttga tggattattt taattgactt tagtgggtta gatgccagtt tggatttttg
      121 atatttgtgt tgagtttaaa agataaaaac acttaatgaa aatctgtttt ttcttagaaa
      181 ggggaccagt atgattgaga cgctaatgag tacgttggaa atggttagaa aaaaaatcct
      241 agtaggactg caatcaaaga tatgacaaaa gggctctcct atcgtgaagt ggatatagta
      301 actaatagaa tggctaatca aatcatgcaa aagataggag taaaccgacg tgttgttgtt
      361 atcttaccac acgggattaa tcaaatatta gccctcattg caatattaaa atcgaataat
      421 tgttatgttc caaaaggggg aatatctagg ttctttttca gataaatctg aattcttaag
      481 tagacatcca aaaatttata tcatctcatt taggtataaa aatcaatttt atattctttc
      541 taacaaaaaa attgatttat aaaaagtttt agaatagagg ttaacatgaa aattacagtt
      601 gtaggcattg gatacgttgg attatcgata ggactcctac ttgcaaagga acacgacgtt
      661 accttttttg atattgataa taaaaaaatt gatttaataa ataaaaggca atcctctctt
      721 aaagaagcag ctataaacaa acttttatgt aaggcaaaaa atattaatgc aacttcttct
      781 gaagaattag catataagga tgcgactttc ataatcttgt ctttgccaac caacctaaaa
      841 gttaataagc ttgatacttc cattatcgaa atttctgtaa gtaatatttt aaagataaac
      901 aaaaaggcta caattgtaat aaagtcgaca gttccaattg gttttacaga atatttaagg
      961 aatcgatttc actacaacga tatcattttt tcacctgagt tccttaggga aggatcaact
     1021 attcatgatc aattgtatcc ttcgagaact atagttggaa atgaatctag aaattctcaa
     1081 ttattcttag acatactaac agatatatcg gttgaaaaag actcgccatc tttattagtt
     1141 ggctcttctg aagcagaagc gataaagtta ttttcgaatg catacttggc acaaaaaatt
     1201 gcttttttta atgagttgga tacgtttgct gaaatgcaaa atttggactc aaaaaaaatt
     1261 attgaggcta tgggatatga ccagagaata ggaaattcgc acaataatcc ttctttcggt
     1321 tttggtgggt actgtcttcc taaggatatt aagcaattag agtatcattt taaagaaatt
     1381 ccagcaccaa t
//
LOCUS       DAUDYX010000109         1369 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.111, whole genome shotgun sequence.
ACCESSION   DAUDYX010000109 DAUDYX010000000
VERSION     DAUDYX010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.111"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(86..1207)
                     /gene="mnmA"
                     /locus_tag="VHD02_001891"
     CDS             complement(86..1207)
                     /gene="mnmA"
                     /locus_tag="VHD02_001891"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEQ5498464.1"
                     /translation="MTDNSKIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVKRDENGTVHMLRGADNGKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKSEVREIAERAGLATAKKKDSTGICFIGEKNFKQFLSQYLPAQ
                     KGRMMTIDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNQPWFVVGKDLSQNILYVGQ
                     GFYHEALMSNSLDASVIHFTREMPEEFTFECTAKFRYRQPDSHVTVHVRGDKAEVVFA
                     EPQRAITPGQAVVFYDGKECLGGGMIDMAYKNGQPCQYI"
BASE COUNT          416 a          301 c          233 g          419 t
ORIGIN      
        1 gcatgattgg tttttataaa taatatcata tgatgtcaaa ggaattgttg aatgtccctt
       61 tctttttatc ttaataaaag gttcattaaa tgtactgaca aggctgccca tttttataag
      121 ccatgtcaat cataccacca ccaaggcatt cttttccgtc atagaaaaca acagcttgac
      181 caggagtaat cgcgcgctgt ggctctgcaa aaacaacttc tgccttatct cctcgaacat
      241 gcaccgtcac gtgtgaatcg ggttgacgat agcgaaattt agcagtacat tcaaatgtga
      301 actcttctgg catctcacgt gtaaaatgga tgactgaggc gtcaagacta tttgacatta
      361 gggcttcatg gtagaaacct tgaccaacgt ataaaatatt ttgcgacaaa tctttaccga
      421 caacaaacca aggttgatta tccccaccat gctgaccacc aatgcccaga ccaccacgct
      481 gaccaatagt ataatacata aggccggcat gttctcccat atcgcgacca tcaatagtca
      541 tcatgcgacc tttttgtgcc ggtaagtact ggctcaaaaa ctgtttgaaa ttcttttcac
      601 caataaaaca aatacctgtt gaatctttct ttttagccgt tgcaagccct gctcgctcag
      661 cgatttctct aacttctgac ttttgcaaat gaccaagggg gaataaagtc ttttgtagtt
      721 gttcctgtga tagttgactt aaaaagtagg tttggtcttt gccattatca gctccacgta
      781 acatgtgaac cgtaccattt tcatcacgct tgacttgagc atagtgtccc gttgctacat
      841 aatctgcacc taaagtcatg gcatagtcaa ggaaagcttt aaattttatt tccttattac
      901 acatgacatc gggatttgga gtacgacctg ctcggtattc ggccaagaaa tattcaaata
      961 ctctgtccca atattctttt tcaaaattga ctgaatagta aggaataccg atttgatcag
     1021 caacggctgc tacatctttg taatcttcag tagctgtgca aacaccaaat tcatctgtat
     1081 cgtcccagtt tttcatgaag acaccaataa catcataacc ttgctccttt aaaagtagag
     1141 ctgttacaga tgaatcaacg ccaccactca taccaacgac aacacgtatc tttgagttat
     1201 ctgtcataaa aaattctccc atcaataata agatagaaaa agataatcat catattatga
     1261 atattatgat tctttttctt atcagttaac gtacgattga aggtcgtcgt tttcaaaagt
     1321 cgatttgaag gtcgattttg aagcgccatc agcgcaaacc ttattataa
//
LOCUS       DAUDYX010000110         1326 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.112, whole genome shotgun sequence.
ACCESSION   DAUDYX010000110 DAUDYX010000000
VERSION     DAUDYX010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.112"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            389..505
                     /locus_tag="VHD02_001892"
                     /pseudo
     CDS             389..505
                     /locus_tag="VHD02_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611996.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(691..>1326)
                     /locus_tag="VHD02_001893"
     CDS             complement(691..>1326)
                     /locus_tag="VHD02_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEQ5498465.1"
                     /translation="KEAAKANLEKVAKETKALISGDRYLSETEKAAQKQAVEQALAKA
                     LGQVEAAKTVEAVKLAENLGTVAIRSAYVAGLAKDTDQATAALNEAKQAAIEALKQAA
                     AETLAKITTDAKLTEAQKAEQSENVSLALKTAIATVRSAQSIASVKEAKDKGITAIRA
                     AYVPNKAVAKSSSANHLPKSGDANSIVLVGLGVMSLLLGMVLYSKKKESKD"
BASE COUNT          417 a          258 c          216 g          435 t
ORIGIN      
        1 gtaaaccctt tctttttaat attatcaaaa atgacaaaaa aaataataat tgattaatat
       61 agaaacaatt atgatctaat atactgaatt ttataataga ttctatcaat aactatgtgt
      121 aaacaatcaa gtagaaatgt ctttattaag cgaatcaatc aaggttgtag agtaaatgct
      181 ggactagatt tcatgtagaa attagtaaaa gttaggagat tttttggtcg tttcaaacga
      241 taacatcatc ttcttataac aagaacttca ctcagagata aactattctc atactttagt
      301 agcctctttt gagcttgtag cacgaaaatt cgctcgaact gatttaaaat aggcatttta
      361 tctgctcgct tgatccctac tgcgcaacca agaaaccttt tattcactag aacaattaac
      421 ccttaaaacc aaggaggaag tccactggtg gaactaccat caccttcaca gtaatcttaa
      481 ttatcaaaca cctataacta aatgagtcat cgtttgaaca aaaaacgcct tataaattct
      541 gtacagaaaa ctgttgcctt ttcaattgaa ctttgaaatg ttttcaatac actccgttct
      601 tgagccctta cgatccaaaa aaccaaggtg atttcaaaat caccttggtt tgaaaatata
      661 gtagaaagca atttggataa agccaggttc ttagtcttta ctttcttttt tcttgctata
      721 aagcaccata cctaaaagaa gagacataac tcctaagcca acaagaacaa ttgagtttgc
      781 atcacctgat tttggaagat ggttcgctga cgatgatttt gcgactgcct tattaggcac
      841 ataggctgca cggatagcag taataccttt atcttttgct tctttcacag acgcaataga
      901 ttgtgctgaa cgaacagtcg caatagccgt cttaagcgct aatgatacat tttctgattg
      961 ttcagctttt tgagcttcag tcaatttagc atcagttgta atcttagcaa gtgtttctgc
     1021 cgcagcttgt ttaagagctt caatagcagc ttgtttcgct tcgttaagag cagctgttgc
     1081 ttgatcagta tctttagcta aaccagcaac atatgctgaa cggatagcta cagtaccaag
     1141 gttttctgcc aacttaacag cttcaactgt cttagcagcc tcaacttgac caagtgcttt
     1201 cgcaagagct tgctcaacag cttgtttttg ggctgctttt tcagtttcgc tcaagtaacg
     1261 gtctcctgaa ataagagctt tagtttcttt agctactttt tcaagattag ctttagcagc
     1321 ttcttt
//
LOCUS       DAUDYX010000111         1299 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.113, whole genome shotgun sequence.
ACCESSION   DAUDYX010000111 DAUDYX010000000
VERSION     DAUDYX010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.113"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..539
                     /locus_tag="VHD02_001894"
     CDS             <1..539
                     /locus_tag="VHD02_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEQ5498466.1"
                     /translation="TVKDAISDLPKLFPLEHDINFNGRRTSYTLPEPFIQNHVARWHS
                     ERDKKVFKLLTTDIESGRNEYVSTEKLKELYMQITGKNSNVHKYYVLRWDKPSNLIPA
                     HLYKDGLRHIHPDSSQLRTLTVREAARLQTFPDDYVFYGSNTETYKMIGNAVPPLFSK
                     KLALALKEFLDKYDQGGK"
     gene            536..>1299
                     /locus_tag="VHD02_001895"
     CDS             536..>1299
                     /locus_tag="VHD02_001895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498467.1"
                     /translation="MMAYTAKDAVQTFLNYINLKKENIEFIPETSNRGLILDEDDEKV
                     VIFVYPISHKADDSKNFFDTRDSGARERGIAWEYALAKDLKYFCVAVHDEVDRYKDYI
                     FSLESNESIIATISGTLGGKRNGPGTQVVIPNDFAPKKNIERIKTKNNFYITAVHKEA
                     FFNYLKVFDNRPYTHEYDIETLESFSQIESHYEEFDLSEEFARYLRTVDKIEESTAIS
                     YVGSLRGKLFNEIKEDVGDIDIYSISSINKFSEVIK"
BASE COUNT          489 a          159 c          252 g          399 t
ORIGIN      
        1 caacagtaaa agatgcaata agtgatttac ctaagctttt tccattggaa catgacataa
       61 attttaatgg gaggagaaca tcatatactt tgccagaacc ttttatacaa aatcatgttg
      121 ctaggtggca tagtgaaaga gataagaagg tttttaagct tttaacaaca gatatagaga
      181 gtggtagaaa tgaatatgtt tctaccgaaa aactcaaaga attatatatg caaataacag
      241 gtaaaaattc aaatgtacat aagtattatg tgctgcgttg ggataagcca agtaacttaa
      301 taccggctca tttatataaa gatggattaa gacatattca tccagattct agccaattaa
      361 gaacgttgac tgttcgtgaa gctgcaaggc tacaaacatt tcctgatgat tatgtttttt
      421 atggtagtaa tacggaaaca tacaaaatga tagggaatgc tgttccaccg ttgttttcaa
      481 aaaaattagc actagcattg aaagagtttt tagataaata tgatcaggga ggtaaatgat
      541 ggcatacaca gctaaagatg cggttcaaac atttttgaac tatataaact tgaaaaaaga
      601 aaatattgag tttattcctg aaactagtaa tagaggtttg attttagatg aggatgatga
      661 aaaagtagtc atatttgttt atccaattag ccataaagca gatgattcaa aaaatttttt
      721 tgatacaaga gatagtgggg caagagaacg tggaattgcc tgggaatatg cacttgcaaa
      781 agatttgaaa tatttttgtg tggccgttca tgacgaagta gacagatata aagattatat
      841 tttcagcttg gagtcaaatg agtcaattat agcaaccatt tctggaactc ttgggggaaa
      901 aagaaatggt ccaggaactc aagttgttat tccgaatgat tttgctccta agaaaaacat
      961 tgagagaatt aagactaaaa ataatttcta tataacggct gttcataaag aggcattctt
     1021 taactattta aaggtgtttg acaatagacc gtatactcat gagtatgata tagaaacatt
     1081 agaaagtttc tcgcaaattg aatcgcatta tgaagaattt gatttatcag aagagttcgc
     1141 tagatacttg agaacagttg ataaaattga agaatctaca gcaataagtt atgtaggctc
     1201 tcttagagga aagcttttta atgaaattaa agaagatgtt ggagatattg atatttatag
     1261 catctcaagt ataaataaat tttctgaggt tataaaaaa
//
LOCUS       DAUDYX010000112         1280 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.114, whole genome shotgun sequence.
ACCESSION   DAUDYX010000112 DAUDYX010000000
VERSION     DAUDYX010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.114"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..642
                     /locus_tag="VHD02_001896"
     CDS             <1..642
                     /locus_tag="VHD02_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HEQ5498468.1"
                     /translation="INCDSIENNFSLYLSVVQDLLPQLIESCIISDIEVSSYKPEVNR
                     YGGPHLISYAEEIFCKESILFLNNTIEFSDDERLVCATYLVLYYLNHFFKDKETRRSF
                     LFENYTGKYKKDFKNLPIDLPIEYMKILNGVTSALDRYDFFQEMDRYLMSYMKEYNRF
                     GGTNDIYNTKFNLVGSFLHLSMNRLNGIDREFEEKVYCFAYYTLNAQQYIEWE"
     gene            648..>1280
                     /locus_tag="VHD02_001897"
     CDS             648..>1280
                     /locus_tag="VHD02_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HEQ5498469.1"
                     /translation="MLKIQNLKKSYGKRTILNNVNMNIPKGKVYALIGPNGAGKSTIM
                     KILTGLVSKTSGSIIFEGREWSRRDLRKIGSIIEEPPLYKNLSAYDNMKVVTTMLGVS
                     ESAILPLLNKVGLGNIDKRPVKQFSLGMKQRLGIAISLINSPKLLILDEPTNGLDPIG
                     IQELREIIESFKSEGMTIMISSHILSEVEHLADFIGFIYEGKIVLEKEYDG"
BASE COUNT          465 a          156 c          234 g          425 t
ORIGIN      
        1 ataaattgtg attcgattga aaataacttt tctttgtatt tgtcagtagt tcaagatcta
       61 ttacctcaac tgattgagag ttgtattatc tctgacatag aagtgagtag ctataaacca
      121 gaagtaaata gatatggagg acctcattta ataagttatg cagaggaaat tttttgtaaa
      181 gaatctatct tatttcttaa taatacaata gaattttcag atgatgaaag attagtttgt
      241 gctacttact tggttctata ttatctaaat cactttttca aagataaaga aacaaggcgc
      301 tctttcttat ttgagaatta taccggaaag tataagaaag atttcaaaaa tttgccaatt
      361 gatttaccta ttgagtatat gaagattttg aatggagtca cctcagctct tgatagatat
      421 gattttttcc aagaaatgga tagatatctc atgtcttata tgaaagagta taatcgattt
      481 ggtggtacaa atgatatcta taatactaaa ttcaatttag taggaagttt tttacatcta
      541 tcaatgaatc gattaaacgg gattgataga gagtttgaag agaaagtgta ttgttttgct
      601 tactatacac taaacgctca gcaatatata gaatgggagt aactaatatg ttgaaaatac
      661 aaaatctaaa aaaatcttat ggtaagagaa ccatattgaa taatgtcaat atgaatattc
      721 ctaagggaaa agtgtatgct ttaatcggtc caaatggtgc tggaaaatca actattatga
      781 aaattttgac aggcttagtt agtaagacaa gtggttctat tatttttgaa ggtagagaat
      841 ggtcacgtcg ggatctgcga aaaatcggga gtattattga agaaccacca ctttataaaa
      901 atttgagtgc ttacgataat atgaaggtag ttacaacaat gcttggtgtt tcagaaagcg
      961 ctatacttcc attattaaat aaagttggtc taggaaatat tgacaagaga ccagtaaaac
     1021 aattttctct tggaatgaag caacggttag gtatagctat ctctttaata aattcaccta
     1081 aattacttat attagacgaa cctactaatg gcttggaccc aattggaatt caagaattaa
     1141 gggaaattat agagtcattt aaatcagaag gaatgacaat tatgatttca agccatatac
     1201 tgtcagaagt tgaacatcta gctgatttta ttggatttat ctatgaagga aagattgttc
     1261 tggaaaaaga atatgacggc
//
LOCUS       DAUDYX010000113         1268 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.115, whole genome shotgun sequence.
ACCESSION   DAUDYX010000113 DAUDYX010000000
VERSION     DAUDYX010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.115"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..>1268
                     /locus_tag="VHD02_001898"
     CDS             <1..>1268
                     /locus_tag="VHD02_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronidase"
                     /protein_id="HEQ5498470.1"
                     /translation="EGLKATMANDKIGLQAEIQTTAQGLYQRYDNEIRKLSAKITTTS
                     SGTTEAYESKLDGLRAEFTRSNQGTRTELESQISGLRAVQQTTASQISQEIRDRTGAV
                     SRVQQDLESYQRRLQDAEDNYSSLTHTVRGLQSDVGSPTGKIQSRFTQLQYQIDQRVT
                     RDGVMSIINQSGDSIKLAIQKAGGINAKMSGSEIISAINLNSYGVQIAGKQIALDGNT
                     VVNGTFTTKIAEAIKIRADQIIAGTLDASKARIINLNASSIVGLDANFIKAKIGYAIV
                     DMLEGKVIKARNSAMLIDLSSAKMDFNSNATINFNSRDNALVRKDGTHTAFVHFSNAT
                     PKGYTGSALYASIGITSSGDGVNSASSGRFAGLRSFRYATGYNHTAAVDQTELYGDNV
                     LIADDFSINRGFKFRPDKMEKVLDMNDLYAA"
BASE COUNT          400 a          269 c          294 g          305 t
ORIGIN      
        1 gaggggctaa aagccacaat ggccaatgat aaaatcggtt tacaagctga gatacaaaca
       61 accgctcaag gcttgtatca aaggtatgat aacgagatac gcaagctgtc cgctaaaata
      121 accactacct cgtcaggcac tacagaggcc tatgaaagca agctcgatgg cttacgagct
      181 gagtttactc gctcaaatca aggcacgagg acagagctcg agtcacaaat tagcggacta
      241 agagcggtac aacagacaac cgctagccaa atctcacaag agattagaga caggacagga
      301 gcagtcagtc gtgtgcagca agacctagag agttatcagc gtcgtttgca ggatgcggaa
      361 gataattaca gtagcttaac ccatacagtt agaggtttgc agagtgatgt gggatccccg
      421 actggtaaaa tccaatcacg ctttacgcag ctgcaatacc aaatagacca gcgagtgact
      481 cgtgacggag ttatgtcaat cattaaccag tctggagaca gcattaaatt agctatccaa
      541 aaggctggcg gcattaatgc caagatgtct ggcagcgaaa tcatctcagc gattaactta
      601 aactcctacg gtgtgcaaat cgctggtaag cagatagctt tagatggtaa cacggtagtt
      661 aacggcacct ttaccacaaa aatagccgag gctatcaaaa tcagagctga ccaaatcata
      721 gcaggcactt tagatgcctc taaagctcgt attattaatc taaacgccag cagtatcgtt
      781 ggtttagacg ctaactttat caaagctaaa attggctatg cgattgttga catgcttgag
      841 ggtaaagtga ttaaggcacg caatagcgct atgcttattg atcttagctc ggctaagatg
      901 gattttaata gtaatgcgac catcaacttt aacagccgag ataacgcttt agtgcgtaaa
      961 gacggtaccc acactgcctt tgtacacttt agtaatgcca caccaaaagg ttatacaggt
     1021 tcggcattat atgcctctat tggtatcacc tcatctggtg atggggtcaa cagtgcgtca
     1081 tctggacgtt ttgcagggct aaggtcattt aggtatgcta cgggatataa tcatactgct
     1141 gcagtcgacc aaaccgagct atacggtgat aatgtcttga ttgcagatga ctttagcatc
     1201 aatcgaggat ttaaatttag accagacaaa atggaaaaag tgctcgacat gaacgacttg
     1261 tatgcggc
//
LOCUS       DAUDYX010000114         1265 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.116, whole genome shotgun sequence.
ACCESSION   DAUDYX010000114 DAUDYX010000000
VERSION     DAUDYX010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.116"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(<1..>1265)
                     /locus_tag="VHD02_001899"
     CDS             complement(<1..>1265)
                     /locus_tag="VHD02_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronidase"
                     /protein_id="HEQ5498471.1"
                     /translation="EGLKATMANDKIGLQAEIKASAQGLSQKYDDELRKLSAKITTTS
                     SGTTEAYESKLAGLRAEFTRSNQGTRTELESQISGLRAVQQTTASQISQEIRNREGAV
                     SRVQQGLDSYQRRLQSAEGNYNSLRETVAGYERRISNQDNTISSNFTQLKTLIDQSVT
                     LEKVQSLLRQSGDSIMLAIKDKLPKSKMSGNEIISAINLNSYGVTIAGKHIALDGNTT
                     VNGTFTTKIAEAIKIRADQIIAGTIDAARIRVINLNASSIVGLDANFIKAKIGYAITD
                     LLEGKVIKARNGAMLIDLSSAKMDFNSNATINFNSRDNALVRRDDTHTAFVHFSNATP
                     KGYTGSALYAAIGITSSGDGVNSASSGRFAGMRCFRHATGYNHTAAVDQTEIYGDSVL
                     IADDFSINRGFKFRPDKMTKVLDMNDLYAA"
BASE COUNT          307 a          300 c          275 g          383 t
ORIGIN      
        1 gccgcataca agtcattcat atcgagtact ttagtcattt tgtccggcct aaatttaaag
       61 cctctattga tgctaaagtc atctgcaata agtacactat caccataaat ttctgtttgg
      121 tcgactgccg cagtgtggtt gtagcctgta gcgtgcctaa agcaacgcat acccgcaaaa
      181 cgtccagatg acgcactgtt gaccccatca ccagatgagg tgatacctat ggctgcatag
      241 agtgccgaac ctgtataacc ttttggtgtg gcattactaa aatgtacaaa agcagtgtgt
      301 gtgtcgtccc tgcggaccag agcgttatca cgactgttaa agttgatggt cgcattacta
      361 ttaaaatcca tcttagccga gctaagatca ataagcatag cgccattacg agctttgatg
      421 actttgccct cgagcaaatc agtgatagca tagccaattt tagctttgat aaagttagcg
      481 tctaaaccaa cgatactact tgcgttaagg ttaattactc taatcctagc agcgtcaatc
      541 gtgcctgcaa taatctgatc agccctaatc ttgatagcct ctgctatctt tgtggtaaag
      601 gtgccgttga cagtcgtatt gccatcgaga gcgatgtgtt tacctgcgat tgttactccg
      661 taggagttga ggttaattgc tgagataatc tcattaccag acatcttgct cttaggcaac
      721 ttgtccttaa tcgcgagcat gatactatca ccagattgcc gcaagagcga ctggaccttc
      781 tccaaggtca cagactgatc tatcaaagtc tttagctgag taaagttaga ggagatagtg
      841 ttatcctgat tggatattct gcgctcataa cccgctacag tctctctcaa actgttgtaa
      901 ttaccctctg cggactgtaa tcgtcgttgg taactgtcta ggccctgttg tacacgactg
      961 acagcacctt cacggttacg tatctcttgt gagatttggc tagcggttgt ctgttgtacc
     1021 gctcttagtc cgctaatttg tgactcgagc tctgtcctcg tgccttgatt tgagcgagta
     1081 aactcagcac gtaagcccgc aagcttactc tcgtaggcct cggtcgtgcc gcttgaggtt
     1141 gttgtgatct tagccgatag cttgcgcaac tcatcatcat acttttgcga tagcccttgt
     1201 gctgaggctt taatctcagc ttgtaaaccg attttatcat tggccattgt ggcttttagc
     1261 ccctc
//
LOCUS       DAUDYX010000115         1207 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.117, whole genome shotgun sequence.
ACCESSION   DAUDYX010000115 DAUDYX010000000
VERSION     DAUDYX010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.117"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(<1..1153)
                     /locus_tag="VHD02_001900"
     CDS             complement(join(<1..823,823..1153))
                     /locus_tag="VHD02_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEQ5498472.1"
                     /translation="MIRLIDRYGVTIVQKCKNHYYSPELKQKIINKVLIDGQSQKQTS
                     LDYALPTSSMLSRWIAQYKKNGYTILEKPRGRPSKMGRKRKKNLEEMTEVERLQKELE
                     YLRAENAVPKKAERIPLEGRSKAQRATEIIQALRNPFPLEMLLEILDLSRSTYYYQVK
                     RLAQGDKDIELKHVIREIYDEHKGNYGYRRIHMELRNRGFVVNHKKVQRLMKVMGLAA
                     RIRRKRKYSSYKGEVGKKADNLIKRHFEGSKPYEKCYTDVTELALPEGKLYLLPVLDG
                     YNSEIIDFTLSRSPNLKQVQTMLEKTFPADSYSGTILHSDQGWQYQHQSYHDFLESKG
                     ILPSTSRKGNSPDNGMMESFFGILKSEMFYGLETTYQSLDKLEEAITDYI"
BASE COUNT          326 a          253 c          224 g          404 t
ORIGIN      
        1 aaaatgtaat ctgtaatagc ttcttcaagc ttgtcaagtg attgataagt tgtctcgagt
       61 ccataaaaca tttcagattt gagaatgcca aagaaggatt ccatcatgcc gttatccggg
      121 ctattccctt tgcgggacgt ggatggcaaa atacctttag actctaaaaa atcatggtag
      181 gactggtgtt ggtattgcca cccctggtca ctatgaagaa tagttccgct gtatgaatcc
      241 gctggaaaag tcttctcaag catggtttga acctgtttca agttaggaga ccgagacaga
      301 gtgaaatcaa taatctcact gttatagccg tcaagaacag gtaataagta gagttttcct
      361 tcaggtaagg ccaactccgt cacatcagtg taacattttt cgtaaggttt agagccttcg
      421 aagtgacgtt taatcagatt atcagccttc ttaccaacct ctcctttgta agaagaatac
      481 ttgcgcttcc gacgaatgcg ggctgctagg cccatgactt tcatgagacg ttgaactttc
      541 ttatgattga ccacaaatcc tcggttacgc aactccatat gaatacgacg ataaccgtaa
      601 ttacctttat gctcatcata aatctctcga atcacatgct ttagttctat atccttatct
      661 ccttgagcta gtcgcttcac ttgatagtaa taggtggagc gtgataaatc aaggatttca
      721 agtagcatct ctaagggaaa tggattcctt aatgcttgaa tgatttctgt tgctctttga
      781 gctttacttc gtccctcaag cggtattctc tcagcttttt tagcacagca ttctccgctc
      841 taaggtattc taattccttc tgaagacgtt caacctctgt catctcttcc aaatttttct
      901 tacgtttacg ccccatctta ctcggtctcc ctcttggttt ttcaagaata gtataaccgt
      961 ttttcttgta ttgcgctatc caccttgaga gcatactaga agttggtaaa gcataatcta
     1021 aggatgtttg tttctgagat tggccgtcaa tcaggacttt atttattatc ttttgcttca
     1081 gttctggaga ataataatga ttcttacatt tttgaacaat ggttacccca tacctatcaa
     1141 tcaagcgaat catatatttg agatcggatt ctgccatatc aaactttttt gatatggatt
     1201 taatgga
//
LOCUS       DAUDYX010000116         1193 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.118, whole genome shotgun sequence.
ACCESSION   DAUDYX010000116 DAUDYX010000000
VERSION     DAUDYX010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.118"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            113..>1193
                     /locus_tag="VHD02_001901"
     CDS             113..>1193
                     /locus_tag="VHD02_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEQ5498473.1"
                     /translation="MQIVQGVQLHLIKTKQFKTNHITFRFSGDLNQKTVAKRVLVAQM
                     LATANECYPTVRQFREKLARLYGASLSTNVLTKGLVHIVDIDITFIQDRYACNGEKIL
                     DEMIQFLKDILFSPLLSIAQYQPKVFETEKNNLINYIESDREDSFYYSSLKVKELFYC
                     NKNLQMSEYGSPELIAKETAYTSYQEFHKMLNEDQIDIFILGDFDDYRVVQLIHQFPL
                     DNRNKYLNFFHLQNSVNIIKESIEKRAVHQSILQLAYHFPSVFGQRDYYALVLLNGLL
                     GSFAHSRLFIKIREEEGLAYSIGCRFDSYTGLFEIYTGIDSQHRTKTLQLIIQELNAI
                     KMGRFSEQLIKKTRSMLLNNALLSED"
BASE COUNT          424 a          188 c          190 g          391 t
ORIGIN      
        1 tattttacta acactagaat cccgacaatt aagtcaaaaa aagttatcac tcacctaacc
       61 tgaataaaaa tggtataatc gtaaggatca cactatttat taaggaaaca ttatgcaaat
      121 tgttcaggga gtacaacttc atctgattaa gaccaaacag tttaaaacca atcatattac
      181 ctttcgtttt tcaggtgatt tgaaccaaaa aacagtagca aaaagagtct tagtggcaca
      241 gatgttagca acagctaatg agtgttatcc aactgttagg cagtttagag aaaaattagc
      301 acgactttat ggagctagct tgtcaactaa cgtgttaaca aaagggttag ttcatattgt
      361 ggatatcgat attactttta ttcaagatag atatgcttgc aatggggaaa aaatactaga
      421 tgaaatgatt cagtttttaa aagatatctt gttctcaccc ctattatcca tcgcccagta
      481 tcagcctaaa gtttttgaga ctgaaaaaaa taatttgatt aactacatcg agtcagatag
      541 agaggattct ttttactaca gctctcttaa ggtaaaagaa ttgttttatt gtaataagaa
      601 tctacaaatg tcagaatatg gtagcccaga actaattgca aaagagactg cctatactag
      661 ctatcaagaa tttcataaaa tgctcaatga agatcagatt gatattttca ttttaggaga
      721 ttttgatgat tatcgcgttg ttcagctaat tcatcaattt ccacttgata accgaaataa
      781 atacctaaat ttctttcatt tacaaaattc tgtcaatatt attaaagaat ctattgaaaa
      841 aagagcagtt catcaatcta ttttacaatt agcttatcac ttcccttcag tttttggaca
      901 acgtgactat tatgctttag ttcttttaaa tgggttgtta ggctcttttg cacattcccg
      961 tctctttata aaaattagag aagaagaagg acttgcttat agtatagggt gtcgttttga
     1021 tagttataca ggtttatttg aaatttatac tggaattgac agtcaacatc gcactaaaac
     1081 gttacaacta atcatccaag aattaaatgc cattaaaatg ggacgattct cagaacaatt
     1141 gattaaaaag accagatcga tgttgttgaa taacgcctta ttatcagaag att
//
LOCUS       DAUDYX010000117         1159 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.119, whole genome shotgun sequence.
ACCESSION   DAUDYX010000117 DAUDYX010000000
VERSION     DAUDYX010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.119"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            79..837
                     /gene="mf2"
                     /locus_tag="VHD02_001902"
     CDS             79..837
                     /gene="mf2"
                     /locus_tag="VHD02_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNase Mf2"
                     /protein_id="HEQ5498474.1"
                     /translation="MKLSKQKASLLTAVLLLLSLSITTITVDAARVRTYPNVSHANTH
                     YKNTVSSKLLPFTANYQLQLGELDNLNRATFSHIQLQDRHETKDVRTKINYDPVGWHN
                     YQFPYGDGSKSSWVMNRGHLVGYQFCGLNDEPRNLVAMTAWLNTGAYSGANDSNPEGM
                     LYYENRLDSWLALHPDFWLDYKVTPIYSGNEVVPRQIELQYVGIDSSGELLTIRLNSN
                     KESIDENGVTTVILENSAPNINLDYLNGTATPKN"
     gene            948..>1159
                     /gene="speC"
                     /locus_tag="VHD02_001903"
     CDS             948..>1159
                     /gene="speC"
                     /locus_tag="VHD02_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021733756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeC"
                     /protein_id="HEQ5498475.1"
                     /translation="MKKINIIKIVFIITVILISTISPIIKSDSKKDISNVKSDLLYAY
                     TITPYDYKNCRVNFSTTHTLNIDTQK"
BASE COUNT          420 a          199 c          181 g          359 t
ORIGIN      
        1 aaagtgatat aattaactag aaaagtgcaa tatttgatcc actctaaaag ctaaaaatgg
       61 aaaggtttag gtgttatcat gaaattatct aaacaaaagg caagtttgct taccgctgtt
      121 ttactgttac tttctctttc tataacgaca ataactgttg acgcagctag ggtacgaact
      181 tatccgaatg tatcccatgc aaacacacat tataaaaata ctgtttctag taagctctta
      241 ccttttactg ctaattatca gttacaactt ggtgagttgg ataaccttaa ccgcgctacc
      301 ttttcacata tccaactgca agatagacac gaaactaaag atgtacgcac taaaataaat
      361 tatgaccctg tgggatggca caactatcaa tttccatacg gagatggctc aaaatcttca
      421 tgggtaatga atcgtgggca tctagtcgga tatcaatttt gtggattaaa cgatgaacca
      481 agaaatttag tggcaatgac agcctggcta aacacaggtg cttattcagg agcaaatgac
      541 agcaatcctg aagggatgtt atactatgaa aaccgtttag attcttggct tgcacttcac
      601 cctgactttt ggctagatta taaagtcact cctatatata gtggcaatga agtagtgcct
      661 cgacaaattg aattacagta tgttggaatt gattcatctg gtgagttgct tactataagg
      721 ctaaatagta ataaagaaag tattgatgaa aatggtgtta ctacagtaat cttagaaaac
      781 tctgctccaa atatcaacct tgactattta aatggaactg ccactcctaa aaactaaaat
      841 ataaatacat ttataaaatt tctaaataaa cagaaatctg atttttaact acttactgct
      901 atttcatgta ttctcgtacg agtaatacat ttaattaagg agaaaaaatg aaaaagatta
      961 acatcatcaa aatagttttc ataattacag tcatactgat ttctactatt tcacctatca
     1021 tcaaaagtga ctctaagaaa gacatttcga atgttaaaag tgatttactt tatgcataca
     1081 ctataactcc ttatgattat aaaaattgca gggtaaattt ttcaacgaca cacacattaa
     1141 acattgatac tcaaaaata
//
LOCUS       DAUDYX010000118         1055 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.120, whole genome shotgun sequence.
ACCESSION   DAUDYX010000118 DAUDYX010000000
VERSION     DAUDYX010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.120"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..715
                     /locus_tag="VHD02_001904"
     CDS             <1..715
                     /locus_tag="VHD02_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEQ5498476.1"
                     /translation="GDNLKSLIEREILEKQDLSFQIAALSHDIKTPLTVLKGNLELLE
                     LTTLNKNQEGYIVSMNNSISVFEGYFNSLISYTRMLSEDRSVKLILVEKLLSELHFEV
                     DDLLNINNIEFSICNRLIITSFYGDEENLIRALSNLLVNAIRFMPVLDKKIEVILSES
                     GEQIHFEIWNNGERFSDSTLKKGDKLFYTEDYSRGNKHYGIGLAFVKGVAIKHGGNLQ
                     LNNPARGGASAIISIKKKI"
     gene            872..1012
                     /locus_tag="VHD02_001905"
     CDS             872..1012
                     /locus_tag="VHD02_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic streptin"
                     /protein_id="HEQ5498477.1"
                     /translation="MNNTIKDFDLDLKTNKKDTATPYVGSRYLCTPGSCWKLVCFTTT
                     VK"
BASE COUNT          381 a          132 c          189 g          353 t
ORIGIN      
        1 aggtgataat ttgaaaagtt tgattgagcg agaaatattg gaaaagcaag atctatcctt
       61 tcaaattgca gcactttcgc atgatataaa aacaccttta acggttttga aaggaaatct
      121 tgagttatta gaattgacta ctttaaataa aaatcaggaa ggttatattg tatctatgaa
      181 caatagtatt tctgtttttg aaggctattt caattcttta atatcttata cacgcatgct
      241 ttcagaggat agatcagtga aactaatttt agttgagaag ctattaagtg agttacattt
      301 tgaagtcgat gacttattga atatcaataa tattgaattt tctatttgta atagacttat
      361 tatcacttct ttttatggag atgaggaaaa tctgataaga gctctttcta accttttagt
      421 taatgcaata agatttatgc ccgtattaga taaaaaaatc gaagttatac tgtctgagtc
      481 aggagagcaa attcattttg aaatatggaa taatggtgag cgtttcagtg atagtacact
      541 gaaaaaggga gataaattat tttacacgga agattatagc agaggtaaca aacattatgg
      601 aatcggtttg gcatttgtta aaggcgttgc tatcaaacac ggtgggaatc ttcaattgaa
      661 taatccagct agaggaggtg ctagcgccat aatctcgata aaaaagaaaa tttaatagat
      721 ttgattgaaa ctggagtatt tcaggcaaaa aatcgtaaat ttttgtgatt tttggctaaa
      781 atactttttt tctgaacaaa ttctgaactt ggggtgtcat aataatcatg taaatatatc
      841 ggatgcaaaa acgtaagaga ggaactagaa aatgaataac acaattaaag actttgatct
      901 cgatttgaaa acaaataaaa aagacactgc tacaccttat gttggtagcc gttacctatg
      961 tacccctggt tcttgttgga aattagtttg ctttacaaca actgttaaat aataaagtta
     1021 tcattatcat caacattata atttcaaata atttg
//
LOCUS       DAUDYX010000119         1030 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.121, whole genome shotgun sequence.
ACCESSION   DAUDYX010000119 DAUDYX010000000
VERSION     DAUDYX010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.121"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            178..459
                     /locus_tag="VHD02_001906"
     CDS             178..459
                     /locus_tag="VHD02_001906"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ5498478.1"
                     /translation="MTVLNYVNAGDAKSIHNHLFHYTRQVRRHVKVITIGILSAHYYE
                     ITMLFLQAKIILDCFHIPQRLRQAITRFRIRIINTSAIKPIAYRTLKLY"
     gene            complement(936..1023)
                     /locus_tag="VHD02_001907"
     tRNA            complement(936..1023)
                     /locus_tag="VHD02_001907"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(987..989),aa:Ser,seq:gct)
BASE COUNT          354 a          217 c          119 g          340 t
ORIGIN      
        1 gctataagta atacaaaatt gtttatcttc actcagacac cttatccttc ttagaatgaa
       61 tttttagtta cttatccaca ggttaataac tttttttcgc ctaaaatatt atccacaata
      121 gtgacatttt ctattatcag ttatagtata atttcattag gaatctaaaa ccttagcatc
      181 acggttttaa attatgtaaa tgctggggac gcaaaaagca ttcataatca tctctttcat
      241 tataccagac aggtgagaag gcatgtcaaa gttattacca taggcatact cagtgcccac
      301 tactatgaga ttactatgct ttttctacag gctaaaatta tcctcgattg ttttcatatc
      361 ccccaacgtc ttagacaagc tataactcgc tttcgtatcc gaatcataaa tacttctgct
      421 ataaaaccta tcgcgtatag aactctcaaa ctttactgaa aactcataca gcatgattca
      481 cggaagctca tcaataatag ctttattgcc caatttttcg tacgcccata actaacatct
      541 agaaaagaaa agctcttact cacaagatct ctgaatttca ataattattc ctaactctat
      601 caactcttgc tcttcccatt tttcagacaa tttttaaaat ttttctaaaa gaaaagaaaa
      661 caagattagc aatatactta aactcaacta ctctaaggcc aaattagaaa cagtcaacaa
      721 ccttatcaaa gccatcaagt taaacgtttt actcctctac agtagtcaaa gagcctatca
      781 ttttagttat cgaaataatg aacgatttaa atattctatt tagttggttt tgaagattac
      841 aaaaaaaaga cgataaccgt cttctttccc ccttatttat aattcatcct ttaatatgtt
      901 tcatttaaca tattaaccaa aatatcttta caaaacggag aataagggat tcgaaccctt
      961 gcgccagtta cccgacctaa cgatttagca aaccgtcctc ttcagcctct tgagtaattc
     1021 tccaaatata
//
LOCUS       DAUDYX010000120          993 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.122, whole genome shotgun sequence.
ACCESSION   DAUDYX010000120 DAUDYX010000000
VERSION     DAUDYX010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.122"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..444
                     /gene="tilS"
                     /locus_tag="VHD02_001908"
     CDS             <1..444
                     /gene="tilS"
                     /locus_tag="VHD02_001908"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEQ5498479.1"
                     /translation="YLKKDYYIFIDKFSFKITKIVPKTELVKEEKMLEYDSNLCYRDY
                     CFSFMPKSNGDQGQVSIPLFSLSSIKLRSRQSGDYISFGHFSKKIRRLFIDEKFTIAE
                     RQNAIIGEQDEQIIFVLIGNKTYLRKACKHDIMLAKLYIDKLEKG"
     gene            449..991
                     /gene="hpt"
                     /locus_tag="VHD02_001909"
     CDS             449..991
                     /gene="hpt"
                     /locus_tag="VHD02_001909"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEQ5498480.1"
                     /translation="MLEQDIQKILYSENDIIRKTKKLGEQLTKDYQEKNPLMIGVLKG
                     SVPFMAELMKHIDTHVEIDFMVVSSYHGGTSSSGEVKILKDVDTNIEGRDIIIVEDII
                     DTGRTLKYLRDMFKYRKANTIKIATLFDKPEGRVVKIEADYVCYNIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEVYSK"
BASE COUNT          384 a          118 c          169 g          322 t
ORIGIN      
        1 tatctcaaaa aagactatta tatattcatt gataaattct cttttaaaat tacaaaaatc
       61 gttcctaaga ctgagttagt taaagaagaa aaaatgttag aatacgatag taacttatgc
      121 tacagagatt attgtttttc atttatgcca aaaagtaacg gagatcaagg acaggtaagt
      181 atacctttat ttagtttatc gtctattaaa ttaagaagtc gccaatctgg agattatatt
      241 tcttttggtc acttttcaaa aaaaataaga cgtttattta tagatgaaaa attcacaata
      301 gctgaacgtc aaaatgctat tattggagag caagatgagc agattatatt tgtattgata
      361 ggtaataaaa cttatttgag aaaagcttgt aaacatgata taatgttggc taaactgtat
      421 attgataaat tagaaaaagg gtgacagcat gctcgagcaa gatattcaaa aaattcttta
      481 ctctgaaaat gatattattc ggaaaactaa aaaattagga gaacaattaa caaaagatta
      541 tcaagaaaaa aatccgctta tgattggtgt attaaaagga tcagttcctt ttatggcaga
      601 actaatgaaa cacatcgata ctcatgttga aattgatttt atggtagtat caagttatca
      661 tggcggtaca tctagcagtg gtgaagttaa aattttaaaa gatgtagata ccaatattga
      721 aggaagagat atcataattg ttgaagatat tattgacacc ggtcgcacct taaaatattt
      781 acgagatatg tttaagtacc gtaaagctaa tactatcaaa attgcaacac tgtttgataa
      841 acctgaaggt cgtgttgtta aaattgaagc agattatgtt tgttacaata ttccaaatga
      901 atttatcgta ggctttggtt tagattacgc agaaaattat cgcaatcttc cttacgtagg
      961 tgtattaaaa gaagaagtct attcaaaata gaa
//
LOCUS       DAUDYX010000121          967 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.123, whole genome shotgun sequence.
ACCESSION   DAUDYX010000121 DAUDYX010000000
VERSION     DAUDYX010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.123"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(<1..955)
                     /locus_tag="VHD02_001910"
     CDS             complement(<1..955)
                     /locus_tag="VHD02_001910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498481.1"
                     /translation="MEKSRKYLLLAPFLKEVRNIDKIENKPLFYSSNFSPVVNKVIER
                     RIADENDRYLETVKVVNELENTKRLVYFPTVYSLNKFLSGPFEFNIKLGKSIILDEFI
                     EWASNEIHEEWSVLKALNKGILVHHGQLPLGIRMLQLELFDDIDSGYNIMLCTSTLLE
                     GVNTACENIIITKPARNKTEFDAFDFFNLVGRTGRLCQYYLGTAYYIRGTNDREYCKE
                     EALKSIEFELTDNSIDMDINSDRYNEHPEFLSLLQEMGISYEDYIKHFATQFRFSTIN
                     AMYSRFKQNKNLLYKVINDMLVSDKPTKLNVVRELYKIIEND"
BASE COUNT          308 a          174 c          115 g          370 t
ORIGIN      
        1 aatcattttc aatgatttta tatagctccc tcacaacatt tagttttgtt ggtttatcac
       61 ttacgagcat atcattaatt accttgtata ataagttttt attctgtttg aacctactat
      121 acatggcatt tattgttgaa aatctaaatt gtgttgcaaa atgctttata taatcctcat
      181 acgatattcc catctcttgt aacaggctca agaactcagg atgttcatta tatctatcgg
      241 aattaatatc catgtcaata ctattgtcgg ttaattcaaa ttcaatagac tttaaggctt
      301 cttccttaca atactctctg tcgttagtac cgcgtatata atatgcagta cctaaataat
      361 attgacaaag tcttcctgtt ctccctacaa gattaaaaaa atcaaatgca tcaaattcag
      421 ttttatttct tgcaggcttt gttataataa tattttcaca cgcagtatta acaccttcca
      481 ataacgtgga tgtgcatagc attatattat atccactgtc tatatcatca aatagttcta
      541 attgtagcat ccttattcct aaaggtaact gcccgtgatg tactaaaata cctttattta
      601 gtgcctttaa gaccgaccat tcctcgtgta tttcatttga agcccactct ataaattcat
      661 ctaaaattat acttttacca agtttaatat taaattcaaa tggtccagat aaaaatttat
      721 tgagcgaata tactgtgggg aaataaacca gtcgcttggt attctccagt tcatttacaa
      781 cttttacagt ttctaaatat ctatcatttt catcagcaat tcttcgctct ataaccttat
      841 ttacaacagg tgaaaagtta cttgaataaa ataatggttt attttctatt ttatcaatat
      901 ttctgacttc ttttaagaaa ggagctagta acaaatactt tcttgatttt tccattagaa
      961 atttata
//
LOCUS       DAUDYX010000122          913 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.124, whole genome shotgun sequence.
ACCESSION   DAUDYX010000122 DAUDYX010000000
VERSION     DAUDYX010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.124"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(250..>913)
                     /locus_tag="VHD02_001911"
     CDS             complement(250..>913)
                     /locus_tag="VHD02_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HEQ5498482.1"
                     /translation="MLSLLFSALGGESILRLAAETTVNQWGVIWINVIIAVNSGLLNK
                     LELDSNKYSVFLSRDVDLKKVEYARVLLIALINLIISMILSLMLIVISFVLPTPSLIS
                     IGRILLTILLIWLTTLWQIPFILWLSRKINVYFAMIINIISPLIIGTSFSLLNKWYLF
                     PYDWSLKLLEPMTRMRINSIPFGAEFVPDYSQIFISLFLGIAFFILLTNLFAISFKKQ
                     VK"
BASE COUNT          369 a          145 c          121 g          278 t
ORIGIN      
        1 aatatttata ttttccagat gattcttcca aattttttag ttgaatgcac acaatacttg
       61 caacaaaagg aagaagtaca cctactattt gaaagtagga aaataataca gtcctgcttt
      121 gtaaacctcc acgttgactc caatagaaaa atagaagtaa gggaaataat agtgcagcta
      181 aaatatgtat tagtaaatat gaagtatttt tgatttttaa aaaattagaa cataaatatg
      241 atttcatgac tatttcacct gttttttgaa agagatagca aatagattgg tcagtaagat
      301 gaaaaaagca attcctagga acaatgatat aaaaatctgt gagtagtctg gaacaaactc
      361 cgctccaaaa ggtatactat ttattctcat tcttgtcatt ggctcaagca acttcaacga
      421 ccaatcataa gggaacaaat accatttatt taagagagaa aaacttgtac caataattag
      481 tggagatata atattaataa tcatagcaaa atacacatta atttttcttg atagccataa
      541 aataaacggg atttgccata gtgtagtcaa ccaaattaac aagatagtta atagtatcct
      601 acctatacta ataagacttg gagtaggtag gacaaaacta atcacaataa gcattaagct
      661 taaaatcata ctaattatta gatttataag agctatcaat agaactctgg catattctac
      721 cttttttaaa tcaacatccc ttgataaaaa aacagaatac ttattagaat ctaattctaa
      781 tttatttaac aaaccagaat ttacagcaat aataacatta atccaaataa ctccccactg
      841 atttactgtt gtttccgcag ctaaacgtag gatactttca ccacctaatg ctgaaaacaa
      901 taaagataac atc
//
LOCUS       DAUDYX010000123          766 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.125, whole genome shotgun sequence.
ACCESSION   DAUDYX010000123 DAUDYX010000000
VERSION     DAUDYX010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.125"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(150..>766)
                     /locus_tag="VHD02_001912"
     CDS             complement(150..>766)
                     /locus_tag="VHD02_001912"
                     /inference="COORDINATES: protein motif:HMM:NF021104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LlaJI family restriction endonuclease"
                     /protein_id="HEQ5498483.1"
                     /translation="QNILNLLKKYIEEDSGKNSSTNISFVGTNSFNLVWEDVCSEVMD
                     DCINKSIKELGLSYSKNPKQSALISDVIAKPKWKHNISGNVHVANKTLIPDIVTINGD
                     KLSIYDAKYYKIKLDDKGVDKQPGVGDITKQYLYELAYKDFAKENNLVIDSNAFLMPT
                     DGKEEKKVGTASMDIFYGLGDIRLHDIDVILKPCEEMYEIYLEK"
BASE COUNT          245 a          150 c           77 g          294 t
ORIGIN      
        1 tctcatcaaa aatatacgaa actaatcctg tcaagaattt attttcatca tcaaaatccg
       61 catataaaaa actatatttg ttctcctcat tgtaaatctt aaattcttca aaatcgatca
      121 cttccaatat tgctcctcct tcctacaatt tatttttcta aataaatttc atacatttct
      181 tcacatggtt tcaaaataac atctatatca tgaagtcgaa tatcccctaa accataaaat
      241 atgtccatgc ttgccgttcc tactttcttt tcttcttttc cgtcggtcgg cataagaaaa
      301 gcattggaat caattaccaa attgttttcc ttggcaaaat ctttatacgc tagttcgtat
      361 aaatattgct tcgttatatc tcctactccc ggttgtttat caacaccttt atcatctagt
      421 ttgatcttgt aatatttagc atcatatata gataatttat caccatttat tgtgacaata
      481 tcaggaatca atgttttatt tgctacatga acatttccag aaatattatg cttccacttc
      541 ggtttagcaa ttacatctga tattaatgca gattgttttg gattcttaga ataacttaaa
      601 ccaagttctt taatagattt atttatgcaa tcatccatga cttcagagca tacatcttcc
      661 cacactaaat taaagctgtt tgttccaaca aaagaaatat ttgtagatga atttttgcca
      721 gaatcctctt caatatactt tttcaacaaa ttcaaaatat tttgtt
//
LOCUS       DAUDYX010000124          752 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.126, whole genome shotgun sequence.
ACCESSION   DAUDYX010000124 DAUDYX010000000
VERSION     DAUDYX010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.126"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..245
                     /locus_tag="VHD02_001913"
     CDS             <1..245
                     /locus_tag="VHD02_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEQ5498484.1"
                     /translation="ANWAKKGALIGVTGRIQTRNYENQQEQRVYVTEVVAESFQMLES
                     RNSQQQSGQDNSSQNDNSQPFGNSNPMDISDDDLPF"
     gene            251..454
                     /locus_tag="VHD02_001914"
     CDS             251..454
                     /locus_tag="VHD02_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498485.1"
                     /translation="MANRYRQRIYAVYDGDSFVDVGTKHELAERLGVTADTIVFIASP
                     AHKKRRPDGRHAEFIGYEDELEE"
     gene            454..>752
                     /locus_tag="VHD02_001915"
     CDS             454..>752
                     /locus_tag="VHD02_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEQ5498486.1"
                     /translation="MVKFIIPIEPKPQKRPRFSRWSGAYEDGDMMAWRKQVTDYVKNN
                     YEGPYFDDGLKVDVTFYLKAPELVSKKPSERAKDKTKQKYQDYINELLYVPKKPD"
BASE COUNT          258 a          132 c          180 g          182 t
ORIGIN      
        1 tagccaactg ggctaaaaaa ggtgctttga tcggagttac gggtcgtatt cagacacgta
       61 actacgaaaa ccaacaagaa caacgtgtct atgtgacgga agttgttgca gagagtttcc
      121 aaatgttgga aagtcgaaat agccagcaac aatctggtca agataactct tcgcaaaacg
      181 ataacagtca accgtttggc aattcaaacc caatggatat ttcagacgat gatctgccgt
      241 tttaaatgat atggctaaca gatatagaca aaggatatat gcggtctacg acggagactc
      301 ttttgtagat gttggcacaa agcatgagct agcagaaaga cttggtgtta ccgctgacac
      361 aatagttttt atagcgtcgc cagctcataa aaagaggaga ccagatggca gacacgcaga
      421 gtttatagga tacgaagacg aattggagga gtagtggtta agtttataat accgattgag
      481 ccaaaaccgc aaaaacgtcc acgctttagc agatggagtg gagcttacga ggatggagat
      541 atgatggcat ggcggaagca ggtcacagac tatgttaaaa acaattacga agggccttat
      601 tttgacgacg gtttaaaagt tgatgttact ttttatctaa aagcaccaga attggtgtct
      661 aaaaagccgt cagagcgtgc caaagataag actaaacaaa agtatcagga ttatataaat
      721 gagctcttat atgtgccaaa gaagcccgat tt
//
LOCUS       DAUDYX010000125          719 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.127, whole genome shotgun sequence.
ACCESSION   DAUDYX010000125 DAUDYX010000000
VERSION     DAUDYX010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.127"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..202
                     /gene="speC"
                     /locus_tag="VHD02_001916"
     CDS             <1..202
                     /gene="speC"
                     /locus_tag="VHD02_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021733756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeC"
                     /protein_id="HEQ5498487.1"
                     /translation="NYKIYDATSPYVSGRIEIGTKDGKHEQIDLFDSPNEGTRSDIFA
                     KYKDNRIINMKNFSHFDIYLEK"
     gene            complement(270..>719)
                     /locus_tag="VHD02_001917"
     CDS             complement(270..>719)
                     /locus_tag="VHD02_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEQ5498488.1"
                     /translation="IEDSDGYTMRTVEQNIDGNPDALYVGAPARFNTRDFTGVIGWFY
                     PPYQGDTVTQPVSTEPQTSDTIVETAKTGTFTLDVAEINIRRWPSLASEVVGIYKQGD
                     TVSFDSEGYANGYYWISYVGGSGMRNYLGIGQTDKDGNRISLWGKLN"
BASE COUNT          217 a          158 c          138 g          206 t
ORIGIN      
        1 taattataaa atttatgacg ctacttctcc ttatgtaagc ggcagaatcg aaattggcac
       61 aaaagatggg aaacatgagc aaatagactt atttgactca ccaaatgaag ggactagatc
      121 agatattttt gcaaaatata aagataatag aattatcaat atgaagaact ttagtcattt
      181 cgatatttat cttgaaaaat aattcatcat acacaaaaaa ccgcccagaa taatctgagc
      241 ggttttgtct tatctcggag ctttacctcc taatttaatt taccccaaag gctgatgcga
      301 ttcccatctt tatcagtctg tccaatacct aggtagttac gcatacctga gcctccaaca
      361 tagctaatcc aataatagcc attagcgtag ccctcgctat caaagctgac agtatcacct
      421 tgcttgtaga tacctacaac ctcgctggct agacttggcc agcgtctgat attgatctct
      481 gcaacatcaa gggtaaaggt gcctgttttt gctgtctcta cgatagtgtc agaagtttgc
      541 ggctcggtgc tgactggttg cgtgactgta tccccttgat atggtgggta aaaccaacct
      601 atcacgccag taaagtcacg agtgttaaaa cgagctggtg caccgacata caaagcatca
      661 ggattgccat caatgttttg ctcgacagtg cgcatggtgt aaccgtcact atcctcgat
//
LOCUS       DAUDYX010000126          668 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.128, whole genome shotgun sequence.
ACCESSION   DAUDYX010000126 DAUDYX010000000
VERSION     DAUDYX010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.128"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            36..221
                     /locus_tag="VHD02_001918"
     CDS             36..221
                     /locus_tag="VHD02_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498489.1"
                     /translation="MNKWFKKVAIKTIKTMAQTAVGLIGSSVLITDINWPTMLSAVLL
                     SGLTCVLMNVSQIKEEE"
     gene            334..>668
                     /locus_tag="VHD02_001919"
     CDS             334..>668
                     /locus_tag="VHD02_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEQ5498490.1"
                     /translation="MAFLDKIKSAVIAEWHTHKILPSLTAAQAILESGWGKHAPHNAL
                     FGIKADSSWTGKSFDTKTQEEYQAGVVTDIVDRFRAYDSWDESIADHGQFLVDNPRYE
                     AVIGETDYK"
BASE COUNT          219 a          115 c          154 g          180 t
ORIGIN      
        1 cgtattatta cgctatttaa aaaaggagaa gaaaaatgaa taaatggttt aaaaaagtag
       61 caatcaaaac aatcaaaaca atggcacaaa cagctgttgg tcttatcggg tcaagcgtgt
      121 tgattacgga tattaactgg ccaacgatgt tgtcagcggt attactatca ggactaactt
      181 gtgtcctgat gaatgtgtca caaattaaag aagaggagta accatgagaa aagcaatcac
      241 acaactagcc gtcatcatag ctatcatagt gctatatttt ccactggccg tgattgcttt
      301 gattttggct ccatttatag gagaggatga tagatggcat ttttagacaa aataaaaagc
      361 gcagttatcg cagagtggca cactcataag attttgccat ctctgacagc tgctcaagcc
      421 attttagaga gcgggtgggg caaacatgcc ccacacaacg ctctgtttgg tattaaggca
      481 gatagctctt ggactggtaa atcatttgat accaaaaccc aagaggaata tcaagcaggt
      541 gttgtcacgg atattgtgga ccgatttagg gcgtatgata gttgggatga gtcgatagct
      601 gatcacggac aatttttagt tgataatcca cgctatgagg cagttattgg ggagactgac
      661 tataaaaa
//
LOCUS       DAUDYX010000127          664 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.129, whole genome shotgun sequence.
ACCESSION   DAUDYX010000127 DAUDYX010000000
VERSION     DAUDYX010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.129"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..>664
                     /locus_tag="VHD02_001920"
     CDS             <1..>664
                     /locus_tag="VHD02_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498491.1"
                     /translation="QKVTSEEELNGTNLFYWLVTISRVDTESPIILANLEKNIDVRKR
                     EIEHGDNEGLVVDTRLLFDPFRQILVVYNQRGTINNYDLRRFFCQIIGVRGLKFDIIL
                     NSDAFKRVGKLDVVNSISYTVASPTNFKEFRDDTQSENADLKFANSMLGESMQVVIKS
                     NHLSKKNIFDKFSDMLVNDSVNVKNAKVEGFTDGHPELIDLIKNKLEYKGTIFYENTL
                     DDE"
BASE COUNT          248 a           70 c          130 g          216 t
ORIGIN      
        1 gcagaaggta acaagtgaag aagaattaaa tggaacgaat ctattttatt ggttagtcac
       61 catttcacgg gtggacacag aatctccaat tattcttgcg aatcttgaaa aaaatattga
      121 tgtaagaaaa agggaaattg aacatgggga taatgagggc cttgtggttg atacaagatt
      181 attatttgat ccatttagac aaattttagt tgtttataat caaagaggta caattaataa
      241 ttacgactta agaaggtttt tttgtcaaat aattggagtt agaggattga agtttgatat
      301 tatattaaac agtgatgctt tcaaaagggt tggtaagctt gacgttgtaa attcaattag
      361 ttatactgtt gcaagcccaa caaactttaa ggagtttcga gatgatactc aaagtgaaaa
      421 tgccgattta aagtttgcaa attctatgct aggagagtca atgcaggttg ttataaagtc
      481 aaaccatctt tctaaaaaaa atatatttga taaattttca gatatgttag ttaatgactc
      541 agttaatgta aaaaatgcta aagttgaagg ttttaccgat ggtcatccgg aattaataga
      601 cttaataaaa aataaattgg aatataaggg aactattttt tatgaaaata ctttagacga
      661 tgag
//
LOCUS       DAUDYX010000128          628 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.130, whole genome shotgun sequence.
ACCESSION   DAUDYX010000128 DAUDYX010000000
VERSION     DAUDYX010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.130"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(<1..>628)
                     /locus_tag="VHD02_001921"
     CDS             complement(<1..>628)
                     /locus_tag="VHD02_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEQ5498492.1"
                     /translation="QKLMFSELLSNISFQNSLKLLKESFPLFLNGFLIIYIYTQPKYA
                     IELMTTLGEVALGSQTIFNILFMPAFVMNLLILFFRPYITQMAIALIRGQIKEFNKIQ
                     VQLFAYLGVFSLIALVGSGLFGIPFLSMLYGTNLTDYWVDFMLIMLGGSIGSFATVID
                     NILTAMRKQQLLLIPYTGGFLISLLITNLFVMKYHILGAALSFLITMLV"
BASE COUNT          259 a          104 c           81 g          184 t
ORIGIN      
        1 accaacattg ttattaaaaa gctcaaagca gctcctaaaa tgtgatattt cataacaaaa
       61 agattggtaa ttaataacga aatcaaaaat cctcctgtat atggaataag aagaagttgc
      121 tgttttctca tagcggttaa gatattatcg ataactgtcg caaaactacc aatcgagcct
      181 cctagcataa tcaacataaa gtctacccaa taatccgtaa ggttagtacc atatagcatt
      241 gataaaaagg gaataccaaa caaaccactt ccaactaacg ctatcagtga aaaaactcct
      301 aaataagcaa acaattgaac ttgtatctta ttaaactctt ttatttgacc tctgattaaa
      361 gcaatagcca tctgtgtaat ataaggtcta aaaaataaaa ttagcaaatt cataacaaag
      421 gctggcataa ataaaatatt aaaaattgtt tgagagccca aagcaacttc acctaaagtc
      481 gtcattagtt caatagcgta ttttggttga gtataaatat agataatcaa aaaaccgttt
      541 agaaaaagtg gaaaactttc ttttaataac tttaaactgt tttgaaatga aatattactt
      601 aataattcac taaacatcaa cttttgaa
//
LOCUS       DAUDYX010000129          623 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.131, whole genome shotgun sequence.
ACCESSION   DAUDYX010000129 DAUDYX010000000
VERSION     DAUDYX010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.131"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(<1..574)
                     /gene="rgg4"
                     /locus_tag="VHD02_001922"
     CDS             complement(<1..574)
                     /gene="rgg4"
                     /locus_tag="VHD02_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Rgg4/ComR"
                     /protein_id="HEQ5498493.1"
                     /translation="MLEHFGGKVKVLRLEKRISREDLCGDESELSVRQLARIELGQSI
                     PSLSKVIFIAKALNVSVGYLTDGADLELPKRYKELKYLILRTPTYMDDGKLQVREEQF
                     DEIFEDYYDKLPEEEKIIIDCLQATLDTLLSENTNFGIDLLQEYFNQIKTKVRFRQND
                     LILLELYLAYLDIEGMDGQYSDKIFYDSLLD"
BASE COUNT          202 a          127 c           72 g          222 t
ORIGIN      
        1 tatctaataa agaatcataa aaaatcttat ctgaatattg tccatccatt ccctcaatat
       61 caagataagc taaatataat tctagaagta ttaaatcatt ttgcctaaag cgtaccttag
      121 tctttatttg attaaaatat tcttgaagta agtcaatgcc aaagttagta ttctcactca
      181 ataaagtatc taaagttgcc tgtaaacaat caatgattat tttctcttcc tctggtaatt
      241 tatcataata atcctcaaaa atttcatcaa actgctcttc tcgtacttgt aattttccat
      301 catccatgta agttggtgtc cttaagataa ggtattttaa ttctttgtaa cgcttaggta
      361 gttctaaatc agcaccatca gttaagtaac cgacactaac gtttaaggct tttgcaataa
      421 aaataacctt acttaaactt ggtatggatt gacctagttc tatccgtgct aattgacgaa
      481 cagaaagttc agactcatcc ccacacaagt cctcgcgact aatcctcttt tcaagtctta
      541 acacttttac ttttccacca aaatgttcta acatggtttc tctccaacaa ctgttttctt
      601 tattatatca cagcgtctaa tca
//
LOCUS       DAUDYX010000130          537 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.132, whole genome shotgun sequence.
ACCESSION   DAUDYX010000130 DAUDYX010000000
VERSION     DAUDYX010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.132"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            108..>537
                     /locus_tag="VHD02_001923"
     CDS             108..>537
                     /locus_tag="VHD02_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HEQ5498494.1"
                     /translation="MDLTLKNKELNTLYRVLDKIKITNMRANRGRAKLLAKVVDKINE
                     YAKDETDLIDMYAAKDKDDKFVIDEHKNIKLADPAKLDELNDLLNELADEEIVIKGGE
                     YSKRFIDFLNFLEECEDEFTSSEIILIDNILEQFEESKKGE"
BASE COUNT          208 a           89 c          102 g          138 t
ORIGIN      
        1 tggaagcact tagctaacgt cggctggaat accgttcatg gcaattttac aaatgcggtc
       61 actaacgagt taaataatca tatcaacaaa atttaatagg agaaacaatg gatttaacat
      121 taaaaaataa agaactcaat acactatatc gtgtactaga caaaatcaaa atcactaata
      181 tgcgtgctaa tcgtggccgt gctaagctgc ttgccaaagt agtcgataaa atcaatgagt
      241 atgcaaaaga tgagaccgac ttaattgata tgtatgcagc taaggataaa gatgacaaat
      301 ttgtcattga tgagcacaaa aacatcaagc tagcagaccc cgctaaactc gacgagctca
      361 acgacctact caacgagcta gcagatgaag aaattgtgat taaagggggt gagtactcca
      421 agcgatttat tgacttttta aactttttag aagagtgtga agatgaattt acatcatctg
      481 aaatcattct tatcgacaac attttggaac aatttgaaga aagtaaaaaa ggagaat
//
LOCUS       DAUDYX010000131          526 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.133, whole genome shotgun sequence.
ACCESSION   DAUDYX010000131 DAUDYX010000000
VERSION     DAUDYX010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.133"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(3..76)
                     /locus_tag="VHD02_001924"
     tRNA            complement(3..76)
                     /locus_tag="VHD02_001924"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40..42),aa:Arg,seq:acg)
     gene            complement(93..177)
                     /locus_tag="VHD02_001925"
     tRNA            complement(93..177)
                     /locus_tag="VHD02_001925"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(141..143),aa:Leu,seq:taa)
     gene            complement(186..257)
                     /locus_tag="VHD02_001926"
     tRNA            complement(186..257)
                     /locus_tag="VHD02_001926"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(223..225),aa:Gly,seq:gcc)
     gene            complement(270..342)
                     /locus_tag="VHD02_001927"
     tRNA            complement(270..342)
                     /locus_tag="VHD02_001927"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(307..309),aa:Thr,seq:tgt)
     gene            complement(353..434)
                     /locus_tag="VHD02_001928"
     tRNA            complement(353..434)
                     /locus_tag="VHD02_001928"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(398..400),aa:Leu,seq:tag)
     gene            complement(440..512)
                     /locus_tag="VHD02_001929"
     tRNA            complement(440..512)
                     /locus_tag="VHD02_001929"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(477..479),aa:Lys,seq:ttt)
BASE COUNT          140 a          153 c          111 g          122 t
ORIGIN      
        1 gatgcaccct agaggagtcg aacctctaac cgcctgattc gtagtcaggt actctatcca
       61 gttgagctaa gggtgctatt tcaaaaaatt aatgccgagg accggaatcg aaccggtacg
      121 atgtaaacca tcgcaggatt ttaagtcctg tgcgtctgcc agttccgcca ccccggctcc
      181 tcaaaagcga acgacgggat tcgaacccgc gacccccacc ttggcaaggt gatgttctac
      241 cactgaacta cgttcgcaac taatctcaat gccggctaca tgacttgaac acgcgaccct
      301 ctgattacaa atcagatgct ctaccaactg agctaagccg gctaatcttt tatgcgggtt
      361 aagggacttg aacccccacg ccgttaagcg ccagatccta aatctggtgc gtctgccaat
      421 tccgccaaac ccgcttttat gacccgtact gggctcgaac cagtgaccca ttgattaaaa
      481 gtcaattgct ctaccaactg agctaacgag tctttccttc aaaaaa
//
LOCUS       DAUDYX010000132          525 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.134, whole genome shotgun sequence.
ACCESSION   DAUDYX010000132 DAUDYX010000000
VERSION     DAUDYX010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.134"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..449
                     /locus_tag="VHD02_001930"
     CDS             <1..449
                     /locus_tag="VHD02_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEQ5498495.1"
                     /translation="ANDWFSGGKGNIIMTGKAGRGKSHLAYSIIRGLSDKTKKLGLLV
                     NVTDLLSEIKRDFSKEAFWMDKLKDVDYLVLDDLGAEKVSDWSTSIIYSLLNKRTNTI
                     ITTNLTPAEIRKIYGERIASRIRKGCDKSHIMEFDGMEDERMKLWN"
BASE COUNT          183 a           88 c          126 g          128 t
ORIGIN      
        1 tggctaacga ttggtttagt ggtggtaagg gaaacatcat catgactggc aaagcaggac
       61 gaggcaaaag tcatttagct tatagcatca ttaggggatt gtctgataaa acgaaaaaat
      121 taggcttgct tgtaaatgtc actgacttac tgtcagagat aaagcgagac tttagcaagg
      181 aagcattttg gatggataag ctaaaagatg tcgattatct ggtgctagat gatctaggtg
      241 ccgaaaaagt tagtgactgg tccacaagca ttatttacag cttgcttaac aagcgcacaa
      301 acacaatcat tactaccaat ctgacacctg ccgaaattag aaaaatttat ggagagagaa
      361 ttgcatcccg tatcagaaaa ggctgcgaca aaagtcatat catggaattt gatggcatgg
      421 aggacgaaag gatgaagcta tggaattgac actaacaaca tttttcgggc tatccgagga
      481 acatgcggca aaaatcatgg cgctagatga agatgtccga acaaa
//
LOCUS       DAUDYX010000133          477 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.136, whole genome shotgun sequence.
ACCESSION   DAUDYX010000133 DAUDYX010000000
VERSION     DAUDYX010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.136"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..459
                     /locus_tag="VHD02_001931"
     CDS             <1..459
                     /locus_tag="VHD02_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase"
                     /protein_id="HEQ5498496.1"
                     /translation="KYIEQKYKNISFSIDYHYYLTTTGSCIDFGEEGAVGRGNKTHGI
                     ISSFRPNTMEAPAGKNCTYFVGKVWGFLSDTIAKEIYEAFNTPCQIIMQLNIGSKLYR
                     PTHLFIQTEESVDQERVLEIVNRHLNNGKQNTNLILSTQHFIPKTNVYDG"
BASE COUNT          177 a           61 c           77 g          162 t
ORIGIN      
        1 aaatatattg aacaaaagta taaaaacata agcttttcta ttgattacca ttattattta
       61 actactacag gttcttgtat tgatttcgga gaagaaggtg cagttggtcg aggtaataaa
      121 actcatggta tcatatcaag ttttaggcca aatactatgg aagcaccagc tggaaaaaat
      181 tgtacttatt ttgtaggaaa ggtttgggga tttttgtctg atactattgc caaagaaatt
      241 tatgaagcat tcaatactcc ctgtcaaatc attatgcaat taaatattgg atcaaaattg
      301 tatagaccaa cccatttatt tattcagaca gaagaaagtg ttgatcaaga aagagtatta
      361 gaaattgtta acaggcattt aaataatggt aaacaaaata ctaatttaat cttgtcaact
      421 caacatttta ttcctaaaac caatgtttat gatggataaa actgttttat attttgg
//
LOCUS       DAUDYX010000134          471 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.137, whole genome shotgun sequence.
ACCESSION   DAUDYX010000134 DAUDYX010000000
VERSION     DAUDYX010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.137"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..>471
                     /locus_tag="VHD02_001932"
     CDS             <1..>471
                     /locus_tag="VHD02_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HEQ5498497.1"
                     /translation="LKDKDNPFFKSKYVPLENVTEAITKAFANNGISFSQDPTTNTEN
                     GYIDVATLVMHTSGEWVEYGPLSVKPTKNDVQGAGSAITYAKRYALSAIFGITSDQDD
                     DGNEASKPNKTNQSQKPTNKTSKGASFQTPKISNIQVETYKSDLSDIAKATNQNV"
BASE COUNT          188 a          100 c           87 g           96 t
ORIGIN      
        1 ctaaaagata aagacaaccc ctttttcaaa agcaaatacg tgcctttaga aaacgtgacg
       61 gaagcgatta caaaagcctt tgctaataac gggatatctt tttcgcagga tccaacaaca
      121 aacacagaaa acggttatat cgatgtcgca acgctagtca tgcacacgag tggcgaatgg
      181 gtggaatacg gacctttaag tgttaaacca acaaaaaatg atgtacaagg agctggttcg
      241 gctatcactt acgcaaaacg ctacgcacta tcagcaattt ttgggataac aagcgatcaa
      301 gatgatgacg gcaatgaagc tagcaaaccc aacaaaacaa atcaatcgca aaaaccaaca
      361 aataaaacgt caaaaggggc aagtttccaa accccgaaaa tcagcaatat ccaagtagag
      421 acttataaat ctgatttaag cgatattgca aaagccacaa atcaaaatgt c
//
LOCUS       DAUDYX010000135          449 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.138, whole genome shotgun sequence.
ACCESSION   DAUDYX010000135 DAUDYX010000000
VERSION     DAUDYX010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.138"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            complement(<1..>449)
                     /locus_tag="VHD02_001933"
     CDS             complement(<1..>449)
                     /locus_tag="VHD02_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEQ5498498.1"
                     /translation="QQLIEYDGNGNILVYGRLKYFKEGISLYYIGEYLAECLLIEHSD
                     TLLIHAAAVYNPLSGHSILLLGDKGAGKTTVAIRLCIEHGYHLIGNDQVIFGSNSGIL
                     LTYAGTSFFKIRRTAVLSDNLLFKLFSKFFNRSLQFNKASWDDKISI"
BASE COUNT          155 a           82 c           60 g          152 t
ORIGIN      
        1 aattgaaatt ttatcatccc aacttgcctt attaaattgt aatgaacggt taaaaaattt
       61 gctaaataat ttaaacagta aattgtccga caaaacagct gtccttctta ttttaaagaa
      121 agatgtccct gcataagtta gtagtattcc agagttcgaa ccaaatatta cttggtcatt
      181 accaataagg tgatacccat gttctataca cagccttatt gcaaccgtag tttttccagc
      241 acctttatct cctaatagta aaattgaatg gccagataaa ggattataaa ctgctgctgc
      301 gtgaattaat aaggtgtcac tatgttcaat caagagacat tcagctaagt actctccaat
      361 gtagtataat gaaattcctt ctttaaaata ctttaatctt ccataaacta aaatatttcc
      421 attgccatca tattctataa gttgctgat
//
LOCUS       DAUDYX010000136          414 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.139, whole genome shotgun sequence.
ACCESSION   DAUDYX010000136 DAUDYX010000000
VERSION     DAUDYX010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.139"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            103..>414
                     /locus_tag="VHD02_001934"
     CDS             103..>414
                     /locus_tag="VHD02_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HEQ5498499.1"
                     /translation="MILGDEDALTKIAISYQQNTKKQDAVCEKHGCRYITVLKTGLTV
                     CPDCHREELDNQNTLHVQKQYERELENKRLYYLKKLSIMDDELENASFDNFRADTAKH
                     KE"
BASE COUNT          167 a           70 c           83 g           94 t
ORIGIN      
        1 tgcgaaattg gagacaaaac ggaatcctta cgatggttaa agttgaagaa gaacaaaggc
       61 aattccaata tagaaaatct aaacaagaag aggtatcgga atatgatact tggtgacgaa
      121 gacgcactta ctaaaatcgc tatatcttat caacaaaaca ccaagaaaca agatgctgtc
      181 tgtgaaaaac acggttgtag gtacatcaca gtccttaaga ctggtttgac agtatgtcca
      241 gattgtcaca gagaagaatt agacaatcaa aacactttgc acgtccaaaa gcaatatgag
      301 agagaacttg aaaacaagcg actgtactat ctcaaaaaat tatcaattat ggacgacgag
      361 ttggaaaatg catcatttga caattttagg gctgatacgg ctaaacacaa agag
//
LOCUS       DAUDYX010000137          393 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.140, whole genome shotgun sequence.
ACCESSION   DAUDYX010000137 DAUDYX010000000
VERSION     DAUDYX010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.140"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..343
                     /locus_tag="VHD02_001935"
     CDS             <1..343
                     /locus_tag="VHD02_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HEQ5498500.1"
                     /translation="LKNAQADVEMYKAEKEAFYKKQKQAEAKVEKYKETIRRAMELSQ
                     KKKVDAGMFKVSLRKSKKVEILDETKIPLDYMQEKIEYKPMKSEISKALKSGIDISGV
                     ELIETESLQVK"
BASE COUNT          174 a           51 c           82 g           86 t
ORIGIN      
        1 gttaaaaaat gctcaagctg acgtcgaaat gtataaagcc gaaaaagaag ctttttacaa
       61 aaagcaaaag caagcagaag caaaagtgga aaaatacaaa gagacaatca ggcgtgcaat
      121 ggaattgagc caaaagaaaa aagttgacgc tggaatgttt aaggtgtctt tgcgaaaaag
      181 caaaaaagta gagattttgg acgaaacaaa aattcctctt gattacatgc aagaaaaaat
      241 tgaatacaaa ccaatgaaat ctgagatctc gaaagctttg aaatctggaa ttgatatatc
      301 tggagttgaa ctaatcgaaa cagaaagttt gcaggtaaag taaatgagga aatcagaaag
      361 tataacagaa tatgctaaag ccttttgtaa agc
//
LOCUS       DAUDYX010000138          374 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.141, whole genome shotgun sequence.
ACCESSION   DAUDYX010000138 DAUDYX010000000
VERSION     DAUDYX010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.141"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..>374
                     /locus_tag="VHD02_001936"
     CDS             <1..>374
                     /locus_tag="VHD02_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysin"
                     /protein_id="HEQ5498501.1"
                     /translation="AGYATASSYVELLIQLIEENDLQSWDREALKNNKEETMTTANEI
                     VQYCVNLANSGMGVDKDGAHGTQCCDLPCFVAKNWFGVDLWGNAIDLLDSASAQGWEV
                     HRMPTEANPKAGATFVQSVPYH"
BASE COUNT          114 a           72 c           94 g           94 t
ORIGIN      
        1 gctggatacg ctacggcaag tagctatgtc gaacttttaa tccaactgat tgaggaaaac
       61 gacttacaaa gttgggatag agaagctctt aaaaataata aggaggaaac gatgacaacc
      121 gcaaacgaaa ttgtacaata ctgtgttaac cttgctaatt caggcatggg tgttgacaaa
      181 gacggtgctc acgggacgca atgctgtgac ttgccttgtt ttgtcgctaa aaattggttt
      241 ggtgttgatc tttggggcaa tgcgattgat ttattagaca gcgcaagtgc gcaaggctgg
      301 gaagtccatc gtatgccaac agaggcaaac ccaaaagcag gcgctacatt tgtccaatca
      361 gtgccgtatc atca
//
LOCUS       DAUDYX010000139          357 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.143, whole genome shotgun sequence.
ACCESSION   DAUDYX010000139 DAUDYX010000000
VERSION     DAUDYX010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.143"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..>357
                     /locus_tag="VHD02_001937"
     CDS             <1..>357
                     /locus_tag="VHD02_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEQ5498502.1"
                     /translation="SDIKWLCQNHPKWHKLRGIGMTRNTIDKDGQLSQENRYFIFSFK
                     PDVLTFANCVRGHWQIESMHWLLDVVYHEDHHQTLDKRAAFNLNLIRKMCLYFLKVMV
                     FPKKDLSYRRKQRYISV"
BASE COUNT          108 a           62 c           74 g          113 t
ORIGIN      
        1 ttccgatatc aaatggttgt gtcaaaacca tcccaaatgg cataagttac gtggtattgg
       61 gatgactcgt aacacgattg ataaggatgg tcagctgagt caagagaatc gttattttat
      121 ctttagcttt aagccggatg tcctcacatt tgccaattgt gtacgaggtc attggcagat
      181 agagagtatg cactggttat tggacgttgt ttatcatgaa gatcatcatc agacattgga
      241 taaaagagcc gcatttaacc taaatcttat ccgaaaaatg tgcttatatt ttctcaaagt
      301 gatggtattt cctaaaaaag acctcagtta tcgtcgcaaa caacggtata tttctgt
//
LOCUS       DAUDYX010000140          337 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.144, whole genome shotgun sequence.
ACCESSION   DAUDYX010000140 DAUDYX010000000
VERSION     DAUDYX010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.144"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            61..150
                     /locus_tag="VHD02_001938"
     tRNA            61..150
                     /locus_tag="VHD02_001938"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:97..99,aa:Ser,seq:tga)
     gene            160..233
                     /locus_tag="VHD02_001939"
     tRNA            160..233
                     /locus_tag="VHD02_001939"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:194..196,aa:Met,seq:cat)
     gene            254..326
                     /locus_tag="VHD02_001940"
     tRNA            254..326
                     /locus_tag="VHD02_001940"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:287..289,aa:Phe,seq:gaa)
BASE COUNT           76 a           80 c           92 g           89 t
ORIGIN      
        1 gacaccgcct tttcacggcg gtaacacggg ttcgaatccc gtacggacta tattatattt
       61 ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa aaccgtcagg cgtgtaaaag
      121 cgtgcgtggg ttcgaatccc acatcctcct tttaactatc gcgggatgga gcagttaggt
      181 agctcgtcgg gctcataacc cgaaggtcgt aggttcaaat cctgctcccg caatacataa
      241 gtatgtagtt gttggctcgg tagctcagtt ggtagagcaa tggattgaag ctccatgtgt
      301 cggcggttcg attccgtctc gcgccatgtt atcatat
//
LOCUS       DAUDYX010000141          332 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.denovo.145, whole genome shotgun sequence.
ACCESSION   DAUDYX010000141 DAUDYX010000000
VERSION     DAUDYX010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.denovo.145"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..270
                     /locus_tag="VHD02_001941"
     CDS             <1..270
                     /locus_tag="VHD02_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498503.1"
                     /translation="ICRTFDIKDPKTLRDIIHGMVMKGYLIGSSRRKGGGYYLIDNDD
                     ERAEAISSLESQVRKEQKRINILKNGNLNEFVEIANEVRNGWNQN"
BASE COUNT          118 a           54 c           77 g           83 t
ORIGIN      
        1 atttgtcgaa catttgacat caaagacccg aaaaccttga gagacattat ccacggaatg
       61 gtaatgaaag gctatttgat aggttctagc cgtcgaaaag gcggagggta ctatctaatt
      121 gacaacgatg atgagcgtgc agaagctatc agctcacttg aaagtcaagt tagaaaagaa
      181 caaaagcgga taaacatttt aaaaaacgga aacttgaacg aattcgtaga aatagctaac
      241 gaggtgcgaa atggttggaa tcagaattaa cggtgagctc gtaacctttg atagcaattt
      301 tagagatgcg cttatcttta cgattgactg tc
//
LOCUS       DAUDYX010000142        15293 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.guided.048, whole genome shotgun sequence.
ACCESSION   DAUDYX010000142 DAUDYX010000000
VERSION     DAUDYX010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15293
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.guided.048"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            <1..282
                     /locus_tag="VHD02_001942"
     CDS             <1..282
                     /locus_tag="VHD02_001942"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498504.1"
                     /translation="KYKDTIEVCRTFEDFIKESNISSVIIPIFIAHGDGNKEKIEKFL
                     DRFNSVNSNKMLGLETKYLLISLPVIDKEKFVDFAIRMAVNKAREYECK"
     gene            263..742
                     /locus_tag="VHD02_001943"
     CDS             263..742
                     /locus_tag="VHD02_001943"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEQ5498505.1"
                     /translation="MNTNVNNAQVYRENYILALNNVDNKTEYEKFHADSLFNLGLTMY
                     SREYSFKSAIYNGFACSHIDDNLSLHPEQMRVLNLIKDNNGLIFSAPTSFGKTFVVFE
                     YIAREQPKNVVLIVPTLALVDEYKQKILKKYRDSFLEYKIYTSIDIEKEYDFNLKIA"
     gene            1066..1380
                     /locus_tag="VHD02_001944"
     CDS             1066..1380
                     /locus_tag="VHD02_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEQ5498506.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            1396..1782
                     /locus_tag="VHD02_001945"
     CDS             1396..1782
                     /locus_tag="VHD02_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEQ5498507.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1811..3196
                     /locus_tag="VHD02_001946"
     CDS             1811..3196
                     /locus_tag="VHD02_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEQ5498508.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            3199..3351
                     /locus_tag="VHD02_001947"
     CDS             3199..3351
                     /locus_tag="VHD02_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEQ5498509.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            3374..4579
                     /locus_tag="VHD02_001948"
     CDS             3374..4579
                     /locus_tag="VHD02_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEQ5498510.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPDHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            4622..4843
                     /locus_tag="VHD02_001949"
     CDS             4622..4843
                     /locus_tag="VHD02_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498511.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            4960..5457
                     /locus_tag="VHD02_001950"
     CDS             4960..5457
                     /locus_tag="VHD02_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEQ5498512.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            5432..5938
                     /locus_tag="VHD02_001951"
     CDS             5432..5938
                     /locus_tag="VHD02_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEQ5498513.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            5922..8369
                     /locus_tag="VHD02_001952"
     CDS             5922..8369
                     /locus_tag="VHD02_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEQ5498514.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            8372..10549
                     /locus_tag="VHD02_001953"
     CDS             8372..10549
                     /locus_tag="VHD02_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEQ5498515.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            10546..11547
                     /locus_tag="VHD02_001954"
     CDS             10546..11547
                     /locus_tag="VHD02_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEQ5498516.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            11544..12476
                     /locus_tag="VHD02_001955"
     CDS             11544..12476
                     /locus_tag="VHD02_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEQ5498517.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            12721..12837
                     /locus_tag="VHD02_001956"
     CDS             12721..12837
                     /locus_tag="VHD02_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEQ5498518.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            12853..14772
                     /gene="tet(M)"
                     /locus_tag="VHD02_001957"
     CDS             12853..14772
                     /gene="tet(M)"
                     /locus_tag="VHD02_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEQ5498519.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            14891..15058
                     /locus_tag="VHD02_001958"
     CDS             14891..15058
                     /locus_tag="VHD02_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEQ5498520.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(15118..>15293)
                     /locus_tag="VHD02_001959"
     CDS             complement(15118..>15293)
                     /locus_tag="VHD02_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ5498521.1"
                     /translation="LSIQIPRRRSGPLPANNLVKSTRRLQIEKYMDSFTDKELSLMES
                     LASGINEARNIED"
BASE COUNT         4972 a         2719 c         3195 g         4407 t
ORIGIN      
        1 aagtataaag atacaattga agtctgtaga accttcgaag attttattaa ggaatcaaat
       61 ataagtagcg ttataatacc tatttttatt gctcacggtg atggtaataa agaaaaaata
      121 gaaaagtttt tagatagatt taatagcgtt aattctaata aaatgttagg attagagaca
      181 aaatatctgt taatttcttt accagtaatt gataaggaaa aatttgttga ttttgcaatc
      241 agaatggcgg tgaataaagc tcgtgaatac gaatgtaaat aatgcacagg tgtataggga
      301 aaattacata cttgcgttaa ataatgtaga taataaaacg gaatatgaaa aatttcatgc
      361 agattcttta tttaatttgg gattaacaat gtattcaaga gaatattcat ttaagtcagc
      421 tatttataat gggtttgctt gtagtcatat tgatgacaat ttatcattac atccagagca
      481 aatgcgagtt ttaaatctaa ttaaagataa taatggtctc atctttagtg cgccaacaag
      541 ttttggaaag acgtttgttg tatttgaata tatagcaaga gagcagccta aaaatgttgt
      601 tttgattgta cctactttag cactagttga tgagtacaaa caaaaaatac tcaaaaaata
      661 tagagactcg tttttggagt ataagattta tacgagcatt gatattgaaa aagaatatga
      721 ttttaattta aaaatagcat aaaaatctag ttatccgcat aaaaactgga cttatcacac
      781 tttatcaagg tcaaaaccac tcaatttact actaatttac tacttatgaa tgagctttga
      841 tacgacgatt tatccttgaa aagtgaagat ataaagatac ttccaataaa atttgaatat
      901 ttaataggta gacacttcaa aaaatgaggt gtctattttt ttacccgatt ttgaaaggaa
      961 gtgaacttat gaaaacaaaa aatcaagaat caaaaggtcg ttccccactc tttaagacca
     1021 tcaaacattc attcagccaa taaaaaagaa aggataggta aaaatatgga acttaaattt
     1081 gtgattccca acatggaaaa aacattcggc aatttagaat ttgctggcga ggataaagtc
     1141 gttcagcgaa gaatcaacgg acggctaact gtcttatcaa gaagctataa tctctattct
     1201 gatgttcaaa gagcagatga tattgtggtg gtgcttcctg ctgaagctgg cgaaaaacat
     1261 ttcggctttg aggaacgtgt gaagttagtc aatccacgta ttaccgcaga gggctacaaa
     1321 atcggcactc gtggttttac aaattacctt ttacatgctg acgacatgat aaaagaataa
     1381 agaaagagag gaaaaatgat gagattagca aatggcattg tattagataa agacacgact
     1441 tttggagaat tgaaattctc tgctctacgt cgtgaagtga gaatccaaaa tgaagacggg
     1501 tcggtttcag atgaaatcaa ggaacgtacc tatgacttaa aatccaaagg acaaggacgc
     1561 atgattcaag taagtattcc tgccagcgtg cctttgaaag agtttgatta taacgcacgg
     1621 gtggaactta tcaatcccat tgcggacacc gttgctactg ccacctatca aggagcagat
     1681 gttgactggt atatcaaggc agacgatatt gtgctgacaa aggattctag ttcattcaaa
     1741 gctcaaccac aagcaaagaa agaaccgaca caagacaaat agtcgctagg tagaaaggag
     1801 actttttcgc atgaaacagc gtggtaaaag gattcgccca tctggtaaag atttagtctt
     1861 tcattttacg atagcgtcac tcctgcctgt tttcctgctg gttgtcggac tgtttcatgt
     1921 gaagacaatc cagcagatca actggcagga ttttaaccta tcacaagcag ataagattga
     1981 cattccctat ttaattatca gtttcagtgt cgcaattctt atctgcttgc tggtagcgtt
     2041 tgtattcaaa cgggttcgct atgatacggt taaacaactt taccaccgtc aaaaactggc
     2101 aaagatgata cttgaaaaca agtggtatga atctgaacag gtcaaaacag agggtttctt
     2161 taaagatagt gctggtcgta caaaggaaaa gataacctac ttccctaaaa tgtattatcg
     2221 acttaaaaat ggcttgatac agatacgggt ggaaatcacg ctgggaaaat atcaagacca
     2281 actcttacac ttggaaaaga aattagagag tggcttgtac tgtgagctga cggataaaga
     2341 gttaaaggat tcctatgtgg aatatacttt gctctatgac accatagcca gtcgtatttc
     2401 tattgatgaa gtagaagcta aagatggtaa acttcgctta atgaaaaacg tatggtggga
     2461 atatgataag ctccctcata tgttgattgc tggtggtaca ggtggcggta aaacttactt
     2521 tatactgaca ctgattgaag ccttgcttca tacagattca aaactgtata ttcttgaccc
     2581 gaaaaatgct gaccttgcgg acttaggttc tgtgatggca aatgtctact atagaaaaga
     2641 agacttgctt tcttgcattg aaacattcta tgaagaaatg atgaaacgta gtgaggaaat
     2701 gaagcagatg aagaactata agactggcaa aaattatgct tacttaggtc tcccggcaca
     2761 cttcttaatc tttgatgaat acgtcgcttt catggaaatg ctgggaacaa aagaaaacac
     2821 cgcagttatg aataagctga aacagattgt catgttaggt cgtcaagctg gcttctttct
     2881 aatactggct tgtcaacgtc cagacgcaaa atatttaggc gacggaatcc gtgatcagtt
     2941 taatttcaga gtggctttag gtcgtatgtc tgaaatgggc tatggcatga tgtttggcag
     3001 tgacgtacaa aaggatttct tcttaaagcg aatcaaaggt cgtggctatg ttgatgtagg
     3061 aacaagtgtc atatcagagt tttatactcc ccttgtacca aaaggatatg atttcttgga
     3121 ggaaattaaa aagttatcca acagcagaca gtccacgcag gcgacgtgcg aagcggaagt
     3181 cgcaggtgtg gactgatctt gctggctggt gtggcaatag ccacgccagc acttaacccc
     3241 ccgtatctaa caggggggta caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc
     3301 tttggttaca agggatttac aaaatttcag cgtatgtcaa atgggcttta aaagttgaca
     3361 tacgcctttt tgattggagg gatttttact gaatgaacaa acttggttac agcatttaaa
     3421 agaaaaacgc ttggcttatg gactatctca aaaccgttta gctgttgcga ctggtattac
     3481 aaggcagtat ctaagcgata ttgaaacagg aaaagtcaag ccatcagagg atttacagca
     3541 gtccctttgg gaagctctgg aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga
     3601 ttatgtaagg attcgctttc cgacaacaga cgtacagcag gtggtcgaaa acatcttaca
     3661 actgaaactg tcctattttc ttcatgagga ctatggtttc tattcttatt cagagcatta
     3721 tgctttaggc gacatattcg tcctttgctc ccatgaactg gacaaaggag ttctggtgga
     3781 attgaaaggt cgtgggtgca gacaatttga aagctatctt ctggcacaac aaagaagctg
     3841 gtatgagttc tttatggacg ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc
     3901 cattaacgat aagacaggga ttttgaatat ccctgtactc actgaaaagt gccaacagga
     3961 agaatgtatc tccgtcttcc gcagttttaa aagctatcgc agtggcgaac tggtacgcaa
     4021 agaggaaaag gaatgtatgg gaaacaccct ctatatcggt tcattacaaa gtgaagttta
     4081 tttctgtatc tatgaaaagg actacgagca gtacaagaaa aatgatattc ccattgaaga
     4141 cgcagaagta aaaaaccgtt ttgagattcg attgaaaaat gagcgtgcct attatgcagt
     4201 ccgtgattta ctcgtctatg acaatccaga tcataccgcc tttaaaatta tcaatcggta
     4261 tatccgtttt gtagataaag acgattccaa acctcgttct gattggaaac tgaatgaaga
     4321 atgggcttgg tttattggga acaatcgtga acgattaaaa ctaaccacaa aaccagagcc
     4381 ttactccttc caaaggacgc tgaactggct atctcatcaa gttgccccga ccttaaaggt
     4441 tgcgattaaa cttgatgaaa tcaaccagac gcaggttgta aaagacattc tcgaccatgc
     4501 gaaactgaca gaccgacaca agcagatttt gaagcaacag tcagtaaaag aacaggacgt
     4561 gataacaaca aaaaaataac tcaaatacaa attcattgaa tatagagagg agaacatttt
     4621 tatgaatttt ggacaaaacc tttataactg gtttctatca aacgctcaat cactggtgct
     4681 tttagcaatc gttgtgattg gcttgtatct tggcttcaag cgtgagttta gcaaactgat
     4741 tggcttttta attattgcga ttattgcggt tggcttagtc ttcaacgctg ctggagtaaa
     4801 agacatttta ctagagctat tcaatcgcat tattggtgct taaataaaac cgttcttttg
     4861 tggaatataa gtggttttct tatgttccgc aaaggaatgg tacaccaaac gaagtgcggt
     4921 agggattttt gaatctctac aaagaaagga cgtgaatata tggacgatat gcaagtctat
     4981 attgcgaatt taggcaaata caatgagggc gaattggtcg gtgcgtggtt tacctttccc
     5041 attgactttg aggaagtcaa agagaaaatc ggcttgaatg atgaatatga ggaatacgcc
     5101 attcatgact acgagttacc ctttacggtt gacgaataca cttccattgg cgaactcaat
     5161 cgactatggg aaatggtatc ggaattaccc gaagaattac aatcggagct atctgctctg
     5221 ctcactcatt tttcaagcat tgaagaacta agcgaacatc aagaggatat tatcattcat
     5281 tccgattgtg atgatatgta tgacgtggca cgctactaca ttgaagaaac gggtgcttta
     5341 ggcgaagtac cagctagtct tcaaaactat attgattatc aagcctatgg tcgggattta
     5401 gacctttcag gaacgtttat ctcaaccaat catgggattt ttgaaatcgt ctattaaatc
     5461 tgtcggtaca ttactactgg cagattttct attttacggg gtggctcaat cagctacccc
     5521 tattttttat gaaaggattg attacatgaa gaaaatacga agctatacca gtatctggtc
     5581 tgtggaaaag gtactgtatt ctatcaatga ttttagactt ccgtttccca taacctttac
     5641 gcaaatgaca tggtttgtcg tgtcactctt tgcagtgatg atacttggca acttgccccc
     5701 tctttccatg atagagggag catttctcaa atactttggg attcctgtgg ctttcacatg
     5761 gtttatgtct acaaaaactt ttgatggtaa aaagccttat ggatttttga agtctgtcat
     5821 tgcttatgca ctgcgaccaa agctgaccta tgcaggaaaa aaagtaacgc ttggcagaaa
     5881 ccagccacaa gaagccatta cagcagttag gagtgaattt tatggcatat ccaattaaat
     5941 acattgaaaa caatctcgtc tggaataaag acggggaatg ttatgcttac tatgagcttg
     6001 ttccttacaa ttactcattt ctaagtccag aacagaaaat acaagtgcat gattctttca
     6061 gacagcttat cgcacaaaat cgtgatggca aaattcatgc tttacaaatc agtacagaat
     6121 ccagcatacg ttctgcacaa gagcgttcca aaaatgaagt cactggcaag ctcaaagcgg
     6181 ttgcctatga caaaatcgac caacagacag acgctttaat atccatgatt ggcgaaaatc
     6241 aagtgaacta ccgtttcttt atcggcttta agttgcttct caacgatcag gagttttcta
     6301 tgaaaagtct taccgttgaa gcaaaaaatg ctttgtctga ttttgtctat gatgtgaacc
     6361 ataagctgat gggcgatttt gttagtatga gtaatgatga aatcctgcgt tttcagaaga
     6421 tggaaaagct cttagaaaat aaaatctctc gtcgtttcaa aatccgcagg ttagataagg
     6481 acgacttcgg ctatctgatt gaacaccttt acggacagac aggcactgcc tatgaagagt
     6541 atgagtacca tctatcaaag aaaaagctgg ataatgaaac gctgattaaa tactatgact
     6601 tgattaagcc tactcgctgt ttggtggaag aaaaacagcg atatttgaaa atccagcagg
     6661 aagatgaaac cgtctatgta gcttacttta ccattaacag cattgtcgga gaactggact
     6721 tcccgtcctc tgaaatcttc tactaccagc aacagcaatt tacattcccg attgatacgt
     6781 caatgaatgt ggaaattgta gcgaatcgta aagccctatc tactgtccgc aataaaaaga
     6841 aagaactgaa agacttggat aaccacgctt ggcaaagtga taatgaaacc agctccaatg
     6901 tggcggaagc tctggaaagt gtgaatgagc tggaaaccaa tttagaccaa agcaaggaat
     6961 ctatgtacaa gctgtcttat gtggtaaggg tatcagcaaa tgatcttgac gaactcaaac
     7021 gtcgttgtaa tgaagtgaaa gatttttatg acgatttaag cgtaaaactg gtacgaccat
     7081 ttggggatat gctcggctta catgaagaat ttttacctgc cagcaagcgt tatatgaatg
     7141 attatattca atacgtgacc tctgatttcc tcgctggttt aggttttggt gctactcaaa
     7201 tgctggggga aaatgagggg atttatgttg gctacagctt agatactgga cgcaatgtct
     7261 atctgaaacc tgctcttgcc agtcaagggg ttaagggttc agtaaccaat gcgttagcgt
     7321 cggcttttgt tggttcgctg ggtggtggta aatcctttgc gaataacctt atcgtctatt
     7381 atgcggtgct ttatggggca caagcagtga ttgtagaccc aaaagcagaa cgtggcagat
     7441 ggaaagaaac cttgccagag atttcccatg aaatcaatat cgtcactctg acttctgatg
     7501 agaaaaacaa aggcttactt gacccttatg tgattatgaa aaatcccaaa gattctgaat
     7561 cactggctat tgatattctg acattcctta cggggatttc ctctcgtgat ggggaacgct
     7621 tcccaatcct tagaaaagcc attcgtgcag taaccaatag tgaagtacga gggttgatga
     7681 aagtgattga ggaattacgg gttgagaata cgccactaag taccagtata gccgaccata
     7741 tcgaaagttt tacagactat gactttgcac atttattatt cagtaatggt tatgtggagc
     7801 agtctatcag cttagaaaaa caactgaaca ttatacaggt tgcggacttg gtacttcccg
     7861 acaaggaaac ttcctttgag gaatatacca ctatggagct tttatccgtt gctatgctga
     7921 ttgtcattag tacctttgct ttagacttta tccatacaga ccgaagcatt ttcaagattg
     7981 tagatttaga cgaagcatgg agctttttac aggtagcaca aggaaaaaca ctatctatga
     8041 agctggttcg ggctggtcgt gctatgaacg ctggggtata tttcgtgacc caaaatacag
     8101 acgacctctt agatgaaaaa ctgaaaaata acctcggctt aaaatttgca tttcgttcca
     8161 ctgaccttaa cgagattaaa aagaccttag ccttttttgg tgtagaccca gaggacgaaa
     8221 acaatcagaa gcgattgcgt gatttggaaa acgggcaatg ccttatcagt gatttatatg
     8281 gtcgtgtcgg tgtgatacag ttccaccctg tatttgaaga actgctccat gcctttgata
     8341 ccagaccacc tgtgcgaaaa gaggtgtaaa tgtgaaacca tcaatagtaa acagaataaa
     8401 atcaaactgg acgctgaaac gtctaggtaa agtggcaatg acagtggctt tcacacttgt
     8461 gattgccatt tttcttttag ccatgctggg aacggtggtt caagctgcgg gcttggtaga
     8521 tgatacggtc aatgtggcaa atgaatacag ccgataccca cttgaaaact atcaactgga
     8581 tttttatgtg gataatagct ggggctggct tccgtggaac tggtcggacg ggattggaaa
     8641 acaggtcatg tatggactat atgccattac caattttatt tggacaatca gtttgtatgt
     8701 ttccaatgcg acaggttact tagtacagga agcctattcc ttagacttca tttccgctac
     8761 agcagattcc attggtaaga atatgcagac cttagctggt gtgagtgcaa acggattttc
     8821 aacagagggt ttctatgttg gattcctctt actcttgatt ttggttcttg gggtttatgt
     8881 tgcctatacg ggactgataa agagagaaac cacaaaggca attcatgcca ttatgaattt
     8941 tgtgctggtg tttatcctat cggcttcctt tattgcctac gctcccgact acattaaaaa
     9001 aatcaatgac ttttcatcag acatcagtaa tgccagttta tcacttggca cgaagattgt
     9061 catgccccat tccgatagtc aaggcaagga cagcgtggac ttaatcagag atagcctgtt
     9121 ttccatacag gttcagcaac cgtggctact gcttcaatac aacagttcag acattgaaag
     9181 tatcggtatt gaccgtgtgg aaagcctgct ctccaccagc ccagattcca acaatggcga
     9241 agacagagaa aaaattgttg cggaagaaat tgaagacaga agcaatacca atctaaccat
     9301 tacaaagacc attaaccgtt taggtacagt cttcttccta tttgtcttca atattgggat
     9361 ttccatattt gtattcctat taacaggaat catgattttc tcgcaggtac tttttatcat
     9421 ctatgctatg tttctgcctg tgagctttat tttaagcatg attccatcat ttgatggtat
     9481 gtcaaaacga gccataacaa agctctttaa taccattttg acacgagctg gaatcacatt
     9541 gattattacg acagcattta gtatttcaac catgctctat accttatcgg ctggttatcc
     9601 gttctttttg attgcttttc tacagattgt gacctttgca ggaatctact tcaagctggg
     9661 cgatttaatg agtatgtttt ctctacagag taacgattct caaagtgtgg gaagtcgtgt
     9721 gatgagaaaa cctcgtatgc ttatgcacgc tcacatgcac cgtctacagc ggaaacttgg
     9781 acgttccatg actactctag gggctgggtc tgccattgtt acaggtaaaa aaggacagtc
     9841 gggttcgggg agttctgcaa ggacacaagc agatcactcc cgaccagacg gaaaggaaaa
     9901 atcaacactt ggaaaacgta tcggtcaaac catcggtaca gtagctgata ccaaagacag
     9961 aatggtagac actgctagtg gtttgaaaga acaggttaaa gatttgccga ccaatgcaag
    10021 atatgcagta tatcaaggaa aatccaaagt aaaagagaat gtccgtgatt taaccagtag
    10081 tatttctcaa accaaagcgg acagagccag tggacgcaag gaacagcagg aacaaaggcg
    10141 aaaaaccatt gcgaagcgtc gctctgaaat ggaacaggtc aaacagaaaa aacagcctgc
    10201 ttcttctgtt catgaaagac cgactacaag acaagaacaa tatcatgatg aacagacctc
    10261 aaaacagtct aatattcaga cttcatataa ggaatctcaa caagccaaac aagagcgtcc
    10321 agcagttaag tccgattttt caagtccaaa agtggaacgc caaggcaata ccgttcaaga
    10381 aaaaaccgtt caaaagccag caacttcaac cactacagca gatagaactt cacaacgtcc
    10441 aatcacaaaa gaacgtccgt ctactgttca aagagtacca ctacaaaata caagaagtag
    10501 accaccaatc aaaaccgcca ccattaagaa agtcggtaag aaaccatgaa gttgaaaact
    10561 ttagtgattg gtggttctgg attattcttg atggtcttct cactgcttct gtttgttgcc
    10621 attttatttt cagatgaaca ggacagcgga atttccaata ttcattatgg aggtgtgaat
    10681 gtttccgcag aagtgctggc tcataagcct atggtagaaa aatatgccaa agaatatggc
    10741 gttgaagaat atgtcaacat acttcttgcg attatacagg tggaatcggg cggtactgcg
    10801 gaagatgtta tgcagtcctc ggaatccctc ggtcttccac ctaattcatt gagtacagaa
    10861 gaatccatta agcaaggtgt gaagtatttc agtgaattat tagccagtag cgaaaggctc
    10921 agtgtagatt tagaatcggt tatccagtcc tacaattatg gtggtggttt cttagggtat
    10981 gtggctaatc gtggaaataa atataccttt gaactggctc aaagtttctc aaaagagtat
    11041 tcaggtggcg aaaaagtgtc ttaccccaat cccatagcca tacctatcaa tgggggctgg
    11101 cgatacaact atggcaatat gttttatgtg caactggtaa cgcagtatct tgtcacaaca
    11161 gagtttgatg atgatacggt acaagccatc atggacgaag cactgaaata tgagggctgg
    11221 cgatacgttt acggtggagc ttccccgact acttcttttg attgtagcgg actgacacaa
    11281 tggacgtatg gaaaagctgg aattaactta ccacgaaccg cacaacagca atatgatgtg
    11341 acccagcata tcccactatc ggaagcacaa gctggcgatt tggttttctt tcattctacc
    11401 tataacgctg gctcttatat tactcatgtt gggatatacc ttggcaataa ccgtatgttt
    11461 catgcaggcg acccaatcgg ttatgccgac ttaacaagcc cctactggca acagcattta
    11521 gtgggagcag gacgaatcaa acaatgagaa aggaagattt aatgatgaaa tttagaaaaa
    11581 atcagaataa agaaaaacag ataccaaagg aaaagaaacc tcgtgtctat aaggtcaatc
    11641 ctcataaaaa ggttgtgatt gccttgtggg tacttttagg gcttagtttc agctttgcga
    11701 tattcaagca ctttacagct atagatactc atactattca cgaaacaact atcatagaaa
    11761 aggaatacgt tgatactcat catgtagaaa attttgtaga gaactttgcg aaagtctact
    11821 attcatggga gcaatccgat aagtccattg ataatcgaat ggaaagtcta aaaggctatc
    11881 tgacagatga acttcaagct ctcaatgttg atacagtacg caaagatatt cctgtatcgt
    11941 cttctgtaag aggatttcag atatggacgg tagagccaac tggcgacaat gagtttaatg
    12001 taacctacag tgtagaccag ctcattacag agggagaaaa tacaaagacc gtccactctg
    12061 cttatatagt gagtgtctat gtagatggtt ctggaaatat ggtactggtt aagaatccga
    12121 ccattaccaa catacctaag aaatcaagtt ataaaccaaa agccattgaa agtgagggga
    12181 cggttgattc cattacaacc aatgaaatca atgagttttt aacgacgttc ttcaagctct
    12241 atcctacagc gacagccagt gaactttcct actatgtgaa tgacgggata ttaaaaccaa
    12301 tcggaaaaga gtacatcttt caagaactgg taaatcctat tcacaatcgt aaggataatc
    12361 aagtcacggt atcgctgaca gtggagtata tcgaccagca gaccaaagca acgcaggtat
    12421 ctcaatttga tttggtactt gaaaagaacg ggagtaattg gaagattata gaataacaaa
    12481 tattggtaca ttattacagc tattttgtaa tcacgtactc tctttgataa aaaattggag
    12541 attcctttac aaatatgctc ttacgtgcta ttatttaagt atctatttaa aaggagttaa
    12601 taaatatgcg gcaaggtatt cttaaataaa ctgtcaattt gatagtggga acaaataatt
    12661 ggatgtcctt ttttaggagg gcttagtttt ttgtacccag tttaagaata cctttatcat
    12721 gtgattctaa agtatccaga gaatatctgt atgctttgta tacctatggt tatgcataaa
    12781 aatcccagtg ataaaagtat ttatcactgg gatttttatg cccttttggg tttttgaatg
    12841 gaggaaaatc acatgaaaat tattaatatt ggagttttag ctcatgttga tgcaggaaaa
    12901 actaccttaa cagaaagctt attatataac agtggagcga ttacagaatt aggaagcgtg
    12961 gacaaaggta caacgaggac ggataatacg cttttagaac gtcagagagg aattacaatt
    13021 cagacaggaa taacctcttt tcagtgggaa aatacgaagg tgaacatcat agacacgcca
    13081 ggacatatgg atttcttagc agaagtatat cgttcattat cagttttaga tggggcaatt
    13141 ctactgattt ctgcaaaaga tggcgtacaa gcacaaactc gtatattatt tcatgcactt
    13201 aggaaaatgg ggattcccac aatctttttt atcaataaga ttgaccaaaa tggaattgat
    13261 ttatcaacgg tttatcagga tattaaagag aaactttctg ccgaaattgt aatcaaacag
    13321 aaggtagaac tgtatcctaa tatgtgtgtg acgaacttta ccgaatctga acaatgggat
    13381 acggtaatag agggaaacga tgacctttta gagaaatata tgtccggtaa atcattagaa
    13441 gcattggaac tcgaacaaga ggaaagcata agatttcaga attgttctct gttccctctt
    13501 tatcatggaa gtgcaaaaag taatataggg attgataacc ttatagaagt tattactaat
    13561 aaattttatt catcaacaca tcgaggtccg tctgaacttt gcggaaatgt tttcaaaatt
    13621 gaatatacaa aaaaaagaca acgtcttgca tatatacgcc tttatagtgg agtactacat
    13681 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
    13741 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
    13801 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
    13861 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
    13921 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
    13981 tattacgtgg attctacgac acatgaaatt atactttctt tcttagggaa agtacaaatg
    14041 gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg agatagaact aaaagagcct
    14101 acagtcattt atatggagag accgttaaaa aatgcagaat ataccattca catcgaagtg
    14161 ccgccaaatc ctttctgggc ttccattggt ttatctgtat caccgcttcc gttgggaagt
    14221 ggaatgcagt atgagagctc ggtttctctt ggatacttaa atcaatcatt tcaaaatgca
    14281 gttatggaag ggatacgcta tggttgcgaa caaggattat atggttggaa tgtgacggat
    14341 tgtaaaatct gttttaagta cggtttatac tatagccctg ttagtactcc agcagatttt
    14401 cggatgcttg ctcctattgt attggaacaa gtcttaaaaa aagctggaac agaattgtta
    14461 gagccatatc ttagttttaa aatttatgcg ccacaggaat atctttcacg agcatacaac
    14521 gatgctccta aatattgtgc gaacatcgta gacactcaat tgaaaaataa tgaggtcatt
    14581 cttagtggag aaatccctgc tcggtgtatt caagaatatc gtagtgattt aactttcttt
    14641 acaaatggac gtagtgtttg tttaacagag ttaaaagggt accatgttac taccggtgaa
    14701 cctgtttgcc agccccgtcg tccaaatagt cggatagata aagtacgata tatgttcaat
    14761 aaaataactt agtgtatttt atgttgttat ataaatatgg tttcttgtta aataagatga
    14821 aatatttttt aataaagatt tgaattaaag tgtaaaggag gagatagtta ttataaacta
    14881 caagtggata ttgtgtcctg tatgtggaaa taaaacacga ttaaagataa gggaagatac
    14941 tgaattaaaa aaattccccc tctattgtcc gaaatgcaga caagaaaatt taattgaaat
    15001 aaagcagttc aaagtaactg tgattacaga gccagacgca aagacgcaga gccgataaaa
    15061 tgagattaat acaatctcat tttatcggct ctttccgtta tgtatggatt cttttaatta
    15121 gtcttcgatg tttcttgctt cgttgatacc gctggctaaa gattccatta aggatagttc
    15181 tttgtctgta aagctatcca tgtatttctc tatctgtaat cgtcgggtgc tttttaccaa
    15241 gttattagca ggtaggggtc ccgagcgcct acgaggaatt tgtatcgata aga
//
LOCUS       DAUDYX010000143         1467 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31808
            SAMN14440716-rid18345613.guided.107, whole genome shotgun sequence.
ACCESSION   DAUDYX010000143 DAUDYX010000000
VERSION     DAUDYX010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440716
            Sequence Read Archive: SRR11403624
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731790.2
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/09/2023 22:04:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 26 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 10 of 49
            Pseudo Genes (multiple problems)  :: 11 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440716-rid18345613.guided.107"
                     /strain="MGAS31808"
                     /serotype="emm82"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2016-04"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm82"
     gene            252..335
                     /locus_tag="VHD02_001960"
                     /pseudo
     CDS             252..335
                     /locus_tag="VHD02_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            384..467
                     /locus_tag="VHD02_001961"
     CDS             384..467
                     /locus_tag="VHD02_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEQ5498522.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            592..1329
                     /gene="erm(B)"
                     /locus_tag="VHD02_001962"
     CDS             592..1329
                     /gene="erm(B)"
                     /locus_tag="VHD02_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEQ5498523.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          529 a          221 c          251 g          466 t
ORIGIN      
        1 ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga cggtgacatc
       61 tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa aaaagtggtg
      121 catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt atctatgggt
      181 ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg gtcttgcgta
      241 tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt gtgttgattg
      301 tgcattatct taaaattttg tataatagga attgaagtta aattagatgc taaaaatttg
      361 taattaagaa ggagggattc gtcatgttgg tattccaaat gcgtaatgta gataaaacat
      421 ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata cgttagatta
      481 attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt acaaacaaat
      541 cgtttaactt ctgtatttat ttatagatgt aatcacttca ggagtgatta catgaacaaa
      601 aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca aataataaaa
      661 caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa agggcattta
      721 acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga cagtcatcta
      781 ttcaacttat cgtcagaaaa attaaaactg aacattcgtg tcactttaat tcaccaagat
      841 attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa tattccttac
      901 catttaagca cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc tgacatctat
      961 ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac actagggttg
     1021 ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga atgctttcat
     1081 cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac agatgttcca
     1141 gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg agaatatcgt
     1201 caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt aaacaattta
     1261 agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt atttaacggg
     1321 aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta ctaaagggaa
     1381 tgtagataaa ttattaggta tactactgac agcttccaag gagctaaagg ggtcccgagc
     1441 gcctacgagg aatttgtatc gataaga
//
